LOCUS       DATIPF010000001         8875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1061134, whole genome shotgun sequence.
ACCESSION   DATIPF010000001 DATIPF010000000
VERSION     DATIPF010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8875
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061134"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1177)
                     /locus_tag="VLL06_00005"
     CDS             complement(26..1177)
                     /locus_tag="VLL06_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HSS29377.1"
                     /translation="MSLTTDPPHASVQTEHTNRATDSGTPPALGRAQIAAGSVLTARR
                     WSAVMALLVAISVALFLICLQFGTQWIGLGQILDIVGQVLHDWTIDSDRIGTIGVILM
                     QVRLPRVLLGFLVGACLASVGVVLQALLRNPLADPYVLGVSSGAALGVTLAILLGVGA
                     TVVTFSVLPLCGFVGGLLSLVVVYRMALSYGRLPIHALLLAGVILNAIFSALIMFVTS
                     IMEPNRSVGTMTWLMGTLTAPSYPTLAILACYLLGGLFLLLKQARRLNVLTLGEESAR
                     SLGVDTEHVKRVIYVLSALITGAVVSVSGMIGFIGMVIPHAVRFILGADHRLLLPASA
                     CVGGMFLVIADTLARTLLTPSEIPVGIVTALAGGPFFIYLLAWRKDRFA"
     gene            complement(1183..2073)
                     /locus_tag="VLL06_00010"
     CDS             complement(1183..2073)
                     /locus_tag="VLL06_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HSS29378.1"
                     /translation="MARRQQGILTGMPFMANVTPRTFVDDLGRKIFLAKAPTRVVSLA
                     PSITEILYAIGLTDEIVGVSEFCDYPAAAKQKPKVGYAHPNIEVIISLQPDLVLAPRA
                     FLRAELLAKLEQLKIPTFVVDPESFEEIPSRIQTVGRILNRSASADGVAMTMRQRIAA
                     IRKKLDMVAQVRVLYVLNSQPLITVGPGSYIHQVISLAGGSNIASQATVPYPHLNMEA
                     VLKEDPEIIIFPIGDAEGIPPAEQQEWLRWTSLSAVKQGRLHQISADVLNRPGPRIVE
                     GLEALARVIHPEAFTASIAP"
     regulatory      complement(2267..2518)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2748..4175
                     /locus_tag="VLL06_00015"
     CDS             2748..4175
                     /locus_tag="VLL06_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29379.1"
                     /translation="MKHLIIGIVIVWAFFQLSSLIWSSKGQPPSPTFTRLLEPPQHHS
                     VGPDKNGYFYLLGFTADASLDPGKVGHEIWLETNATPSVSEFNYDKPGRSDLQIQIPI
                     DQVLPAWDSENPLTEFRNMHARIQALSGRDHILSTRYERWLTMPFEDMGFGHRGTPRF
                     AEMLVAHRLYIADGFSRQTALGMERLKKDLYTWRSVLRDATTTATKVMAEIVIADDLH
                     LLSTLLSQPVVNKTVLAMTPNIAPLLTTAESSLRWPLQHQFTLGVHADRVGTPITERQ
                     TDSLNPQEEWLISTARLPKQAFRHIAHPRGPSFLGISLQTRETWEMYATYYDAMIRAT
                     ETGQSILPKIHELAGTGHQGIMESIVSPGPFEPDWDPFHHQLRELDARLRLASLQVAL
                     RRPSAHTTVPNRLAQVGSSYYDPFSGLPMLWSPTQKKIYSVGKDRFDDGGDTSFDISV
                     PAIVSPMPAPRESRSSAPPRQSPHH"
     gene            complement(4413..4670)
                     /locus_tag="VLL06_00020"
     CDS             complement(4413..4670)
                     /locus_tag="VLL06_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HSS29380.1"
                     /translation="MTGIDLLGFAAGTFTTCAFWPQLQKTWVTKSAGDVSLGMLAIFS
                     TGVCLWFLYGLVLHAWPIILTNLVTLLLVGAILVLKMRYRS"
     gene            4819..5010
                     /gene="rpmB"
                     /locus_tag="VLL06_00025"
     CDS             4819..5010
                     /gene="rpmB"
                     /locus_tag="VLL06_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HSS29381.1"
                     /translation="MAFTCDLCSKRRQSGHNVSHANNKTKRVFKPNLQPVRALIDGQP
                     KRIRVCTRCLRSGKVLKAV"
     gene            complement(5072..5386)
                     /locus_tag="VLL06_00030"
     CDS             complement(5072..5386)
                     /locus_tag="VLL06_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29382.1"
                     /translation="MSRRHFWFLVVVTFVGGITGGAFSGWWFAPSPVKAQKANGMNAE
                     EFLLLDMNGKARAGLGLDKNGEVGLVMMSRDGNRKLALSPDDQFAVKLSDQSGRVLWS
                     AP"
     gene            complement(6084..6353)
                     /locus_tag="VLL06_00035"
     CDS             complement(6084..6353)
                     /locus_tag="VLL06_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29383.1"
                     /translation="MCPRVASLLPDVGDALLSEEEVRGLDAARGATFQDVDANCEELE
                     GQHTTVEWKRILGHESLRQSIEAGTYHPYFASEALNIGPTFPKYY"
     gene            complement(6916..7515)
                     /locus_tag="VLL06_00040"
     CDS             complement(6916..7515)
                     /locus_tag="VLL06_00040"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS29384.1"
                     /translation="MKSDQRISPRFSIALVLSMVCLTNSACSNSKPVVDTSQPDRALA
                     QAKELRLSAEAGDAAAQYRLGLLYDEGNGLPQNSLQAKEWFEKAATQGHAGAQVDLGT
                     MYLQGNGAPQSAQMALVWFSRAAEQEEGLAFAKLGRMYVQGRGVLQDFIQAHKWYNLG
                     AAHGDLKAAEARDALAPQMSAAQIAEAQRLAREWKPKAK"
     gene            complement(7639..7989)
                     /locus_tag="VLL06_00045"
     CDS             complement(7639..7989)
                     /locus_tag="VLL06_00045"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HSS29385.1"
                     /translation="MALSSGPAYAEWVFVSGDDEAGMTVYVDPDTIRRQGDFVKMWHL
                     SNRKTTEGYGSIKTQREYDCAEARHRLLAASIFSGHMGQGKLLEDNVKEGLWMPVAPG
                     SSGHALWKVACGKK"
     gene            complement(8087..8782)
                     /locus_tag="VLL06_00050"
     CDS             complement(8087..8782)
                     /locus_tag="VLL06_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSS29386.1"
                     /translation="MRQLLCALGLLLIGVPSAFATPLTWQFDGLLPLGGTVNGSFQYD
                     TDSSPVGFNVRNLANNQTYALTGFSFTITPSVELLPFIPAPFTFSPTTSTSEFCLNHC
                     IFASGNVESFLFNNGQYSMRLAFDTSAIGPLTAPPSRLDQWGPFMANGSELRPVTSGG
                     LIFIPSGQITGPTSVNVPEPATWLLLSLALIGLVGWQTRRRRTAESSHPFSTPPISSV
                     LRGQPLLLPPSSS"
BASE COUNT         2109 a         2648 c         2276 g         1842 t
ORIGIN      
        1 gaatgatcat gctctggaga cagtttcacg cgaaccgatc cttccgccac gccaagagat
       61 agatgaagaa cggtccacca gccagtgctg taacgatccc cacgggaatt tctgacgggg
      121 taagaagcgt ccgtgccaag gtatcggcga taaccaagaa catgcctccg acgcatgccg
      181 atgccggcaa gagcagccga tgatccgccc caaggataaa ccggacggca tgcgggatga
      241 ccatcccaat gaatccaatc atgccactca ccgacaccac ggcgcctgtg atcagcgccg
      301 agagcacata gatcacgcgc ttgacgtgct ctgtgtcaac tcccagtgag cgagcagatt
      361 cctcgcccaa ggtcaacaca ttcaatcgcc gcgcctgctt gagcaagagg aatagaccgc
      421 ccagcagata gcacgccaat atggccaacg tgggatacga cggcgcagtc aatgttccca
      481 tcagccaggt catcgtcccg acggaacgat tcggttccat gatggaggtc acaaacatga
      541 tcagcgctga aaagatcgca ttgagaatca cacctgccaa caagagtgcg tgaatgggta
      601 atcgtccata cgacagcgcc atgcgataca ccaccacgag cgacaacaac cctccaacaa
      661 atccacagag cggtaacaca gagaaggtca cgaccgtagc acctaccccc agtagaatcg
      721 ccagggtgac gcccaatgct gcaccgctcg acacccccag cacataggga tcggctaagg
      781 gattccgtaa caacgcctgc aacacgacac cgacggatgc caagcaggcg cccacgagaa
      841 accccagcaa cacccgagga agtctcacct gcatgagaat cactccgatt gttccgatgc
      901 gatcactgtc aatcgtccag tcatggagaa cctgccccac gatgtcgagg atctgaccga
      961 gcccgatcca ttgcgttccg aactgcaaac aaatcaagaa caatgcgacg gagatcgcca
     1021 caagcaacgc catcacagcg ctccatcgcc tggcggtaag cacagaccca gcagcaatct
     1081 gggccctgcc tagagcagga ggtgtgccgg aatctgtcgc cctattcgtg tgttcggttt
     1141 ggacggatgc atgcggcgga tcggtggtga gactcatacg gctcacggcg cgatactcgc
     1201 agtgaacgct tcagggtgaa tgacccgcgc caacgcctct aatccctcga cgatccgagg
     1261 ccctggacga ttcagcacat cggcagagat ttggtgcagc cgcccctgct ttaccgccga
     1321 caacgaggtc catcgtagcc attcctgttg ttcagctggt ggaatccctt cggcatctcc
     1381 gatggggaag ataatgatct cggggtcttc cttgaggacg gcttccatgt tgaggtgcgg
     1441 gtagggcacg gtcgcctgcg aggcgatgtt ggaaccgccg gctaaactga tcacttgatg
     1501 aatatagcta ccaggcccga cggtaatcag cggctggcta ttcaggacgt agaggacacg
     1561 aacctgcgcc accatgtcca acttcttccg gatcgccgca atgcgttgcc gcatcgtcat
     1621 agccacacca tcggcggagg cggatcgatt gaggatacgg ccgacggttt ggatgcgact
     1681 gggaatttcc tcgaaggact ccgggtcgac gacgaaggta gggattttca attgctccag
     1741 cttggccaag agttcagctc ggaggaatgc acgcggggcc agtaccaaat ctggctgcaa
     1801 gctgatgatg acttcaatat tcggatgggc atatccaact ttgggcttct gtttggccgc
     1861 tgccggataa tcgcagaact cgctgacgcc gacaatctcg tcagttaacc caatggcata
     1921 cagtatttcg gtaatgctag gcgcgagaga gaccactcgt gtgggtgcct tagccaaaaa
     1981 gatcttgcga ccaagatcgt cgacgaaggt acgcggagtc acattcgcca tgaagggcat
     2041 accggtgaga atgccctgct gtcgacgtgc catctccccc tcatcggctt gtgcaaaaga
     2101 accggcacac cacaccccgg aagcaacggc cacaaagagc gtagcagcca cgatccaacg
     2161 gatagggtga cgacttcgcg tactcaaaaa aaacccccaa ggcctcggca gaccctgagg
     2221 attgctcccg caactcatcc tcgccaattc cccagcccac gagggaacgt cggtcaattc
     2281 tcctggcagg tattctggct catggttcgt cctactcccc acgccttccc atccgaagcg
     2341 tcggtcatga cacgtgatac gtaaaaggat cgacgacgtt ccttacgtgt tacgtttcac
     2401 cgttcacgct tcaagacagt ggcactcgca ggtttcgtcc ccatttacag cggcgggacc
     2461 gcgagggatt tgcaccctct tcccataccc aggaggcaca atgtggggag cactctagga
     2521 gaccgtggag aaggttgtca agttcattaa tgtccattgg gccttgccct gctcaagttc
     2581 tgatcgatac ctgtccagac ctatcggcaa acagagcgaa aggccgagac ttttcgctcc
     2641 ccctcccggc ataggctttg ctcacctcta tgatggatga ctcgcgcgaa caggatacat
     2701 gaccacctga acggccaacc cagaatagaa catctacgta cgtatctatg aagcacctga
     2761 tcattggtat tgtcattgtc tgggccttct tccagctctc gagcttgatc tggtcctcca
     2821 aggggcaacc tccgagcccc accttcaccc gactcctgga gccaccgcag caccattccg
     2881 ttggtcccga taagaatggc tacttctatc tccttggctt tactgctgat gcttcactcg
     2941 acccgggcaa ggtcggtcat gagatctggc tggaaaccaa cgccacgcca agcgtgagcg
     3001 aattcaatta tgacaaaccg ggtcgctcgg acctccagat tcagatccca atcgaccagg
     3061 tccttcctgc ctgggactcc gaaaatccat tgactgaatt ccgcaatatg cacgcacgta
     3121 ttcaggcgtt gtccggccgg gatcatatcc tgtccacgcg gtacgaacgc tggctcacca
     3181 tgccgtttga agacatgggg ttcgggcacc gaggcacacc ccgttttgca gagatgctcg
     3241 tggctcaccg cctgtatatc gctgacggat tctctcggca gaccgcactg ggcatggagc
     3301 gactgaaaaa ggatctgtac acatggcgaa gcgtactccg agatgccacg accaccgcca
     3361 caaaagtcat ggcggaaatc gtcatcgccg atgatctcca cctgctttcg actctgttgt
     3421 cgcagcccgt cgtcaacaaa acggtgttgg ccatgacacc caatatcgcc cctctactta
     3481 cgacggcaga atcatcgctc cgctggccgc ttcaacatca attcaccctg ggtgtccatg
     3541 ctgatcgagt aggcacaccg atcacggaga ggcagactga ttcccttaat ccacaggaag
     3601 agtggttgat cagcacagca cgccttccca aacaggcctt tcgccatatt gcacaccctc
     3661 gcggaccctc gttcctagga atctccctcc agacgcgcga aacctgggag atgtatgcca
     3721 cctactatga tgcgatgatc cgggcgacag aaacaggaca gagcattttg ccgaagattc
     3781 atgagcttgc aggtaccgga caccaaggta ttatggaaag tatcgtgagt ccaggcccat
     3841 ttgaaccaga ttgggatccc tttcaccatc agctgaggga gcttgacgcc agactgcgac
     3901 tcgcctcgct acaggttgcc ttacggcgcc cgagcgctca caccaccgtg ccgaatcgac
     3961 tcgctcaagt cggctcgtcc tactacgatc cctttagcgg attaccgatg ttgtggagtc
     4021 cgacgcaaaa gaaaatctac agtgtgggaa aagaccggtt cgacgatgga ggcgacacga
     4081 gttttgatat cagcgtgcct gcaatcgtct cccccatgcc tgcgcctaga gaatcgcggt
     4141 catctgctcc accccgccag agcccacatc attaatttat acgctggcag gatacgactt
     4201 cgcaggatgc tcaaaaagac cgtccagcaa ggccgcagcg agtgaaggac cgaggcgtaa
     4261 ctttctcacc cgcccgcccc gaactgctaa cgcagttctc tcccgatggg tacgttgagg
     4321 gtctgaacga tgcgagaacg ccgcttggga aaaggcgcgc ctcagcgcgc cggggttggg
     4381 cgggtgaaaa gaacggtttt ttcagcatcc tgctaacttc gatagcgcat cttgagcact
     4441 aggatcgccc ctaccagaag caacgtgact aaattagtca gaataatcgg ccaggcatgg
     4501 aggaccaacc catacagaaa ccaaagacaa acacccgttg agaatatggc tagcataccc
     4561 agcgagacat cacccgcaga tttcgtgacc catgtcttct gtagttgagg ccagaaagca
     4621 caggtcgtga acgtccctgc cgcaaacccg aggagatcga tccccgtcat tgtcattcac
     4681 cctttcccag gccccacgtt ctcattcgac gcgggcctac cccgtcaaag tggcttgtca
     4741 agtcgaatag ccctgtggta cgttcttgga ttcgcaatca tatcttgatt cgaatagctt
     4801 agatgaagga gtgtacccgt ggcatttaca tgtgatctct gtagcaaacg tcgacagtcc
     4861 ggccataacg tcagtcacgc caataacaag accaagcggg tcttcaaacc caatcttcaa
     4921 ccggtccgcg cactgatcga cggtcagccg aaacgcattc gggtctgcac gcgctgcttg
     4981 cgtagcggca aggtcctaaa agccgtctag ttgacaggac agccgagact cctggccccg
     5041 cttagcacct cgtgaacact cgccgactcc tctatggagc cgaccagagg acacggccgc
     5101 tctgatcgga cagttttaca gcaaattgat catcgggaga gagggctagt ttcctgttcc
     5161 catcgcggct catcatcacg agacccactt cgccgttctt gtccagtccc aatccagccc
     5221 gtgcctttcc attcatatcc aggagaagaa actcctcagc attcatccca ttggcctttt
     5281 gcgccttgac aggtgaagga gcaaaccacc acccactgaa tgctccacct gtgatccctc
     5341 cgacgaacgt caccacgact aaaaaccaaa agtgccgccg gctcacagcg tctcctttct
     5401 ataacatgta agatctatcg cccgcgctgc tatcctattt cgacttcatc tcatcaaccc
     5461 accatcagga cagacaaaaa aggacttgga agctcatgcc tctcaagtcc tccgtctctc
     5521 acatgagacg cttcacgcct cacgtttcat atgttgcttt ggagcgggcg aggggatttg
     5581 aacccctgac aacttgcttg gcaaacacat taggtaataa caccatgaga catgtcagga
     5641 cacagtatgc aacaggaagc caacaggatc aagtgattcc agtcacggca cgatacaggc
     5701 cggaacagca agacacagga gcaactgtga atccaactgg aaagttcagg tagaaccgtg
     5761 acagaaaatg acactccttt ttcttgcaag cgtgaagacc ctggcatcaa gcggcagctg
     5821 gagtgcggcc ttctgagcct cgacctctag ccatacacac acgcctctct ctcacctggg
     5881 aaaacagggg gacccttctc gagttctact ccttcgacaa aagaaaaccg gctagtagat
     5941 gggacccacg acatagagca aataggcccc agcctttcgt cacgtgctgc tgttctcagt
     6001 ccggatcctc ggtatgtggt ggggccccga cgcgaatacc atgctaggtg aaaccggcac
     6061 acggagagac aagagtgggg ccttcaataa tatttcggaa atgtagggcc tatgttgagg
     6121 gcttcggatg cgaagtacgg atggtacgtg ccggcttcaa tcgattgtct caacgattca
     6181 tgacccagga ttcgcttcca ttcaaccgtg gtgtgttggc cttccagttc ctcgcaattc
     6241 gcatcaacgt cttgaaacgt cgcacccctc gcagcatcga gcccacgcac ctcctcttca
     6301 ctcagaagcg catcacccac gtcaggaagc aaacttgcga ctcgcggaca catactgatt
     6361 tcagcacgcc gccaaaactc gcgccctggg aatccaggaa gcgaacacgt ggctcgcgga
     6421 agaatcttct actgtgccag ttcatcctta ttgcgtcttt gcccgtcccc atagcggctc
     6481 aacagcgtcc aaataggctc gggaggtcat tgggggacaa gccaagactg cctcacactg
     6541 gacagacata caggaatccg tcacttggaa tgaacaggtt ggtcttgccc cacttgcgtt
     6601 cgccaagaca gagatcgcat tcatgaaatc ccaagaagag aacaggctgc caggcatctc
     6661 tgtgtagttc ggttaacttg gtcaacactt gaggatcaac tgtcccgcga ggataaggtt
     6721 gtccgcgttc cagccatcca acagaccgaa gcattgaagg gtgccacgcc ctgaagcagt
     6781 gacagtctgt aaggtctgcg taccagacca tttcacctcc tctcggcctc cttccacatt
     6841 ttcacaatcg tggcaatctc ccacatgttg gagaccgtcg cttcttcaag cgacatgctc
     6901 tcgcgtttga gtcgctcatt tggccttcgg cttccactcc cgtgcgagcc gctgcgcttc
     6961 ggcgatctgg gctgcgctca tctgtggggc gagggcatca cgagcctcgg ctgctttcag
     7021 gtctccatgt gcggctccga ggttgtacca cttgtgcgcc tggataaaat cctggagcac
     7081 gcctcgcccc tgtacataca ttctcccgag tttggcaaag gccagtccct cttcttgctc
     7141 tgccgcccgg ctaaaccaga ccaacgccat ctgggcgctt tgcggagccc cgtttccctg
     7201 gaggtacatt gtgccgaggt ctacttgggc tcctgcgtgc ccctgcgtcg cagccttttc
     7261 aaaccactcc ttcgcttgaa gagagttctg gggtaaaccg ttgccttcgt catacagcag
     7321 accaagtctg tattgagcag cggcgtctcc cgcttcggcg gataggcgga gttcttttgc
     7381 ctgggctagt gcacgatcag gctgagatgt gtccacgaca ggcttactgt tgctgcacgc
     7441 cgaattggtg aggcacacca tcgagaggac tagggcaata ctgaatcgag gcgaaatgcg
     7501 ctgatcgctc ttcatgaatc aatcccctgg ttcctgcgcc agtcgtttgt cgtattaacg
     7561 gctctctcgc aagactctca accccaaacg tctggccagc gagcagagtg tttcacgtcg
     7621 taagtattca ctcatccctc atttcttgcc gcaggcaact ttccacaggg catggccact
     7681 actacctggt gcgactggca tccacaggcc ttcttttacg ttgtcctcaa gcagcttacc
     7741 ttgtcccata tgccccgaga aaattgatgc cgcaagcagc cgatggcgtg cttctgcaca
     7801 gtcgtattcc cgttgagtct tgatagaccc atacccctcc gtggtcttgc ggttgctcaa
     7861 atgccacatc ttcacgaaat ctccttggcg ccgtatggtg tctggatcga cgtacacggt
     7921 cattcccgct tcgtcgtcgc cactcacgaa tacccactcc gcatatgctg gtccactact
     7981 cagtgccatg agcagtatca gtgaccagaa accagagagg taagccagcg gcaagcaata
     8041 atgaggacaa attgagaaag gcattcggag cctccagggt ggcgtatcag gaggaggacg
     8101 ggggaaggag caagggctgg cctctaagga ctgaagaaat gggaggtgtc gagaaaggat
     8161 gcgatgattc ggcggtgcgc cgtctcctcg tctgccagcc gaccagtccg atcagagcca
     8221 gactcaacag tagccatgtt gccggttccg gcacattgac ggacgtcgga ccagtgatct
     8281 gtccgctcgg gatgaatatg agcccaccgg atgtaacggg tcttagttct gagccgttcg
     8341 ccatgaacgg tccccactga tccaacctgg acggcggtgc agtgagcgga cctatcgccg
     8401 acgtatcaaa cgcgagccgc atcgaatact ggccattgtt gaacaggaaa ctctccacat
     8461 tcccactcgc aaagatacag tgattgaggc agaactcaga ggtactcgtc gtaggcgaaa
     8521 aggtgaacgg cgcaggaata aagggcagca gctccacgct tggcgtgatg gtaaacgaaa
     8581 aaccagtcag cgcataggtc tggttgttcg ccaaattccg cacattgaac cccaccgggc
     8641 tgctgtcggt atcgtattgg aatgagccat tgacggtgcc acctaatggc aataggccgt
     8701 cgaactgcca cgtcaggggt gtggcgaagg cggaagggac accgatcagc agcaggccga
     8761 gcgcacacag tagttgtctc atgattggtc gctccttccc tcgcaatggt ggaggatttc
     8821 gtattcagct tgccgcgatc ttcgctgtta atctatcccc ccgaaagagg tagag
//
LOCUS       DATIPF010000002         3948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_88672, whole genome shotgun sequence.
ACCESSION   DATIPF010000002 DATIPF010000000
VERSION     DATIPF010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88672"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="VLL06_00055"
     CDS             <1..1012
                     /locus_tag="VLL06_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29387.1"
                     /translation="TIMYCSPSNAKRREQSGIGYAFTLSAFFLFWLVGSTAFGETPAP
                     SVHWGSLAFPDQYSTLTTGLTLNRFTPIDGTGGTYDSTVSNTLGFNLITFSWTQHSHD
                     RFEGWSFNLTGGIGPTSNEPSEFFQNKLIHQLRHLPPVGSVQPRQDTDAMIDGSITRW
                     FFPNNTGFIGGGFAVGTIYQEQFLRAGIRRLQITPDLYCGEWGDVSLRASGLGRISVQ
                     EDGAVLHQTKQTSRILQPALAIGQYATNARGEQYPTWEIELSATWDSGIFVNGLGQSR
                     KQFFWSAAFTAGPVRFETWNDSLGNVSKTDFGPTYGATLTLDMFRVLAMVHSCSFVYP
                     CS"
     gene            complement(1236..1991)
                     /locus_tag="VLL06_00060"
     CDS             complement(1236..1991)
                     /locus_tag="VLL06_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29388.1"
                     /translation="MSKFDRYAFSASPLFDEAKTREVFSPAVSLKTLVIYCYDPRAAE
                     IPQVVARLFGDEVFPGQVMLDDSGNRAASSTTMFPVIVAGGRAIDALRSITVAQHLFG
                     IQNIVVVHHSYCGATSFTADGIIDAYKLEHNADISELYERGSICIEDYEASLKRDTAL
                     IRSHAGTPKHVNVFGCFYNIDTGLLTEVVKDKAETILIPAASHRGDAINNGTSAPSQD
                     VLPGRTQPPTWNKPCWSMSEKKGKAQSWAIPFL"
     gene            complement(2090..2398)
                     /locus_tag="VLL06_00065"
     CDS             complement(2090..2398)
                     /locus_tag="VLL06_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HSS29389.1"
                     /translation="MYDMKNLSKMKNLETNAPEAMKAFVAFDRAALAEGAIPRKYKEL
                     MALAVAFTTQCPYCIELHANKAREMGASDPEIAEAVLVAAALRAGGAITHGTHAMKGS
                     "
     gene            complement(2463..3284)
                     /locus_tag="VLL06_00070"
     CDS             complement(2463..3284)
                     /locus_tag="VLL06_00070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSS29390.1"
                     /translation="MIASAMSPEVENMKSRLKHIWMAGDYDRFSRYMESGARESYERL
                     RVSPGCQLLDVGCGSGQLALMAAKDGMEVTGVDIAGNSVERARERAQAEGVRARFEEA
                     DAEALPFEDASFDMVASLIGAMFAPRPDLVAKELLRVCKQGGTIALANWTPQGFIGQM
                     FKTVSTFIAPSGMPPPVLWGDEATVRERLGKGLSKLNLVRRQYTLSYPFPPSEVVEFF
                     RLYYGPTNRAFASLDAGGGKHLRQELEALWSSHNRAGMECTTVLAEYLEVIGIRA"
     gene            3491..>3948
                     /locus_tag="VLL06_00075"
     CDS             3491..>3948
                     /locus_tag="VLL06_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSS29391.1"
                     /translation="MDVLSEVLKAVKLDGALFYNAEFSAPWCFRQPASHIMAPYLSSD
                     SKHVIIFHLLTEGRGYTQFDGEGRSTPLNAGDIVIFPHGDPHIMGNGLPVKPVDSAQA
                     LERVLSGGLPVSRHGGGGEITKLICGYMACEPQLSRVFLGGLPPILKINIR"
BASE COUNT          858 a         1160 c         1010 g          920 t
ORIGIN      
        1 caccattatg tattgctcgc cctcaaatgc gaaaagacga gagcaatctg gaataggata
       61 tgcgttcacc ctcagcgcct tcttcctgtt ctggcttgtc ggatcaacgg cgtttggcga
      121 aacacccgct ccctccgtac actgggggag cctcgcgttt ccagatcaat actccacact
      181 caccacggga ttgacgctta accgatttac accgatagac ggaaccggtg gcacatatga
      241 ttccacggtg tccaatacac tgggttttaa tctcatcacg ttcagttgga cgcaacacag
      301 ccatgaccgt tttgaaggat ggagtttcaa tctcacggga ggcattggtc ccacatccaa
      361 tgagccgagc gagttctttc aaaacaagct gattcaccaa ctgcgccact tgccaccggt
      421 tggaagcgtc cagccgcgac aggataccga tgccatgatt gatggatcga tcaccagatg
      481 gtttttcccc aacaatactg ggtttatcgg cggcgggttc gccgtcggca ccatctatca
      541 agaacagttt ctcagggctg gtatccgcag gctccagatc acacctgatt tatactgtgg
      601 agagtggggt gacgtgagcc tacgtgcatc cgggctggga cgcatcagtg tgcaggagga
      661 tggcgcggta ttgcatcaga caaaacagac ttcacgaata ctccagcctg ccctcgcgat
      721 cggacaatac gccacgaatg cacgaggtga acaatacccg acatgggaga tagaactgtc
      781 cgccacatgg gattccggga tattcgtcaa tgggttgggc caaagccgaa aacaattctt
      841 ttggtcagcg gcgttcaccg ccggcccggt gcgttttgaa acctggaatg atagtctggg
      901 gaatgtgagc aagacagact ttggcccgac ctacggcgca accctcaccc ttgatatgtt
      961 tcgtgtattg gcgatggtac acagctgcag cttcgtttac ccgtgctctt agaacaaatc
     1021 gccatgactt tcattcagaa gcaacacagc taagagcatt ttgaaaactg gcgatcaatc
     1081 tacatctctc catatgagtg tctaagggca catttcagac aaccgactgt gctcgccatg
     1141 atctctgcca tatcctttgc gacgtgggtc cacgttactc atacccaaca tgatcagcaa
     1201 caccatcccc acagtaactc acgaagagaa gactgctata aaaatggtat ggcccatgac
     1261 tgtgcctttc ctttcttttc gctcatactc cagcaaggct tgttccatgt agggggttga
     1321 gtcctgcctg gcagaacgtc ctgagacgga gccgaggtgc cattgttgat tgcgtcgccc
     1381 cgatgtgacg ccgcggggat aagaattgtt tcggctttgt ccttcacaac ctcagtcagc
     1441 aagccggtgt cgatgttgta gaaacagccg aagacgttca catgtttcgg cgtccctgca
     1501 tgggaccgaa tcagcgcagt atcccgcttc agcgaagcct cataatcctc gatacagatg
     1561 ctgcctcgct cgtagagttc ggagatgtcg gcattgtgtt cgagtttgta tgcgtcgatg
     1621 atgccgtcgg ccgtgaaact cgtcgcaccg caatatgagt ggtgaacaac aacaatgttc
     1681 tgaattccga aaaggtgttg agccacggtg atcgagcgca gcgcatcgat ggcacgccct
     1741 ccggccacga tgacggggaa catggtggtg gacgatgccg cgcgattgcc cgagtcatcg
     1801 agcatgactt ggcctgggaa tacttcgtcc ccaaatagcc ttgccacaac ctgtgggatt
     1861 tcagccgccc tcggatcata gcaatagatc accagtgtct tgagagaaac ggccggggag
     1921 aagacctctc gtgtttttgc ttcatcaaac aatgggctcg cactgaacgc atatcgatca
     1981 aatttgctca tgtgttgcgc tccttgtgtt gtgacttcag ttcatgatcg ggtatcggaa
     2041 atccatcatc gcggcaatcc gggctttccg ctgcacgtga gcactccggt cagcttccct
     2101 tcatggcgtg tgtaccgtgg gtgatcgctc cccccgcgcg caacgccgca gcgacgagaa
     2161 cagcttcggc gatctccggg tcagaagcgc ccatctctct cgccttgttc gcatggagtt
     2221 cgatgcaata gggacactgt gtcgtgaagg ccacggcgag cgccatcagt tccttatact
     2281 ttctcggaat cgctccctca gctaaggcag ccctgtcgaa ggccacaaac gccttcatcg
     2341 cctcaggcgc attcgtctcg agattcttca tcttgctcag attcttcatg tcatacataa
     2401 ccacctcctg atgacgatcg gttgctgaat gtgatgcgcc ttgccaatcc tcctatgaat
     2461 gttcacgctc gtatgccaat cacttccaga tattcggcga gtaccgtcgt gcactccatc
     2521 cccgctcgat tgtgagaaga ccatagcgct tcgagttcct ggcgaagatg tttaccgccg
     2581 ccggcgtcta gtgaggcaaa cgcacgattg gttggtccgt agtagagacg gaagaactcc
     2641 accacctccg agggaggaaa gggataactc aacgtatact gtcgtctgac cagattgagt
     2701 ttcgacagtc ctttgccgag tctttcacgg actgtggctt cgtcgcccca cagaaccggc
     2761 ggaggcatcc ccgacggcgc gataaatgtc gatacggtct tgaacatctg cccgatgaaa
     2821 ccctgcggag tccaattggc cagggcaatg gttcccccct gcttgcacac ccgcagcaat
     2881 tccttcgcta ccagatccgg ccgtggagca aacatcgccc cgattaagct cgccaccatg
     2941 tcgaaacttg cgtcctcaaa aggtagcgcc tctgcatcgg cttcctcgaa gcgggctcgc
     3001 acaccttccg cttgtgcccg ctcccgtgcc cgctccaccg aattgcccgc aatgtccaca
     3061 ccggtcacct ccatcccatc cttcgcggcc atcaacgcga gctggccgga cccacagccc
     3121 acgtccagca gttggcatcc aggagagact ctgaggcgct cgtaagactc tcgcgcgccg
     3181 ctttccatat aacgcgagaa tcgatcgtag tcaccggcca tccagatatg cttcagacgg
     3241 ctcttcatat tctccacttc tggtgacatc gcactggcaa tcatgttggt cctcctttat
     3301 atgtgtgtgt tcggctcgag gaacgattcg acaaaacccc tgcaaccatg tgtccactat
     3361 aaaggcggta cctggctatt ttcttatccg ggagtctggt tctcttgaca gtggcgtgct
     3421 aggctgcaca tgacttgctt tggcgtccgg tatcatttgc cgacagtctg tcactgcatc
     3481 gtagggcccc atggatgtct tgtccgaagt cctcaaggcc gtcaaactcg acggagccct
     3541 gttttacaat gctgagtttt cggcgccctg gtgcttccgg cagcctgcct cgcacatcat
     3601 ggctccgtat ctttcgtctg attccaagca cgtcatcatc tttcacctgc tgaccgaggg
     3661 ccgcggctac acacagttcg acggagaagg ccggtcgacc cccctcaacg caggagacat
     3721 cgtgatcttc ccgcacggcg atccccacat catgggaaac gggttgcccg tcaagccggt
     3781 ggacagcgcg caagcgttgg agcgggtgtt atctggaggc ctccccgtct cccgccacgg
     3841 cgggggcggg gaaatcacga aactcatctg cggttatatg gcctgtgaac cgcaacttag
     3901 ccgggtcttc cttggcgggc tgccacccat tctgaaaatc aatatccg
//
LOCUS       DATIPF010000003         7957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_442485, whole genome shotgun sequence.
ACCESSION   DATIPF010000003 DATIPF010000000
VERSION     DATIPF010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7957
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442485"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..2557
                     /gene="lon"
                     /locus_tag="VLL06_00080"
     CDS             164..2557
                     /gene="lon"
                     /locus_tag="VLL06_00080"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HSS29392.1"
                     /translation="MNNETLAVKLPVLPIKRTVLFPGIMMPLTVGRERSIAAVEAAMK
                     TEDKTILVVAQRDPLKDAPGLEDLCTIGTKAIVKQIGQSDEGAIHALVQGLERVALLR
                     AEQSEPFLLVQARRLERPADHGTEVQALHRAIQELVTDLPRLVQAPGIQEATVAFSNE
                     DDPIALAYRIASLLNLTVQDEQKLLESSSTVELLRSLYGALSKEIQILQLREKITSDA
                     QTNIGKSQREYILREQLKAIHQELGEGEGEENEITELKKKIQDTDLPDHVRKEVEREL
                     ARLAKVPPSSPDHQVLRTYLELVLELPWKKASEDRLELSTVRQVLEEDHYGIKEVKER
                     IVEHLAVLKLNPTAKAPILCLVGPPGVGKTSLGQSIARAMGRNFERFSLGGMHDEAEL
                     RGHRRTYVGALPGRIIQSMRRAGVNNPVLMLDEVDKMGRDFRGDPAAALLEVLDPAQN
                     HTFRDHYLDLPFDLSKVFFITTANTLDTISQPLLDRMEIIRLQGYSEREKAEIARRYL
                     WPRRLKEAGLEASQALLSDEVLNLVISRYTREAGVRQLEQMLGHLTRKVALTFADLPV
                     DQERQPVTIHADLVGEWLGSERFMQEEARKVLPAGVATGLAWTPSGGDVLYIETTLLP
                     GSHDLTITGQLGDVMQESARAARSYLWSHAESMGLDISRFKRNGVHIHVPSGAIPKDG
                     PSAGITMATALASSYIGEPVRSDTAMTGEISLSGLVLPVGGIKDKVLAAHRAGIKRVI
                     LPKSNEKDLKEVPQEVRDNLTFILVERIEEVLPAAFNHETATHGSFRFGSGIAPASA"
     gene            2714..3133
                     /gene="msrB"
                     /locus_tag="VLL06_00085"
     CDS             2714..3133
                     /gene="msrB"
                     /locus_tag="VLL06_00085"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HSS29393.1"
                     /translation="MPPKIEIGTIVKVVKTDEEWKKILSPAAYRVLRQEDTERPFTDN
                     MHDNKKAGVYSCAGCDLPAYSSEHKFDSGTGWPSFWQPIDPKVVESTTDYKLIFPRTE
                     VHCARCEGHQGHIFKDGPRPTGLRYCINGVALKFTPA"
     gene            3345..4472
                     /locus_tag="VLL06_00090"
     CDS             3345..4472
                     /locus_tag="VLL06_00090"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HSS29394.1"
                     /translation="MKRPRPNTDLAPSIQFQQPAFGDSLQLGKRVLGCLAVCAIAMLS
                     SWIAAPAADAVVSNEYKFKLPLGLEESAMVIPSDNPVTTQKVELGRILFFDKRLSNDN
                     TIACASCHLMQHGFTDGQPVSTGIRGQKGGRSAPASFNRVFSHAQFWDGRAPELEDQS
                     IGPFTNPIEHGFVNYDVMVTKMKKIKGYNTLFLQAFGEDISIGLVGKAIASFQRTVLS
                     GNSPMDRFDQGGEANAISTEAQRGLLLFRDKARCTKCHSGFNLTDEKFHNLGIGWDDN
                     KVDLGRYMVTKNAEELGAFKTPTLREIARTAPYMHDGRFKTLEDVVNFYNQGGVKNPH
                     QDPLILPLELTKEEKHDLVQFLRTLNGEGYKHATAPTSFPQ"
     gene            complement(4772..5152)
                     /locus_tag="VLL06_00095"
     CDS             complement(4772..5152)
                     /locus_tag="VLL06_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29395.1"
                     /translation="MKVRNIFLVAIVLMIPGTVWAYGESGGGSGLIACTAKLSKFSPA
                     NNSEVAPKSEFSFFASHSTNPTSIRVTIKDHLVPVTVTEKHDGFQVTGKLPDAVKGTF
                     AKINVAAKGPNQCDASGGWLVKVK"
     gene            5527..6714
                     /locus_tag="VLL06_00100"
     CDS             5527..6714
                     /locus_tag="VLL06_00100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HSS29396.1"
                     /translation="MLKTLEQLFDKLSDHSPDVCFEVRFWDGKKKSYGTGERQFLLTF
                     ATKRAAEHVIESGSLGFGEEYMSGHIQVEGDFRQLLRFGTDAAFLNLPLGLKTKLAFQ
                     HLRQTSLNTLSRAPKHIAHHYDLGNDFYKLWLDDSMAYSCAYFRHEHDTLEEAQQQKY
                     EHICRKLQLKTGETLVDIGCGWGGMLRYAAKHYGVRGVGCSLSKQQVEYATDLVTREG
                     LSDKVSVVFEDYRKLEGQFDKFVSIGMFEHVGKQFIPTFMEKARSLLKRDGIGLLHTI
                     GEERSMPGDPWTLKYIFPGGYIPSLDEIVRTMGETGLVPTDIENLRLHYAKTVEEWCT
                     RFEAQTKKIETMFDASFVRMWRMFLNGCVVNFRYGNPRLYQVLFTNGLNNTLPLTRDY
                     MYR"
     gene            6823..7563
                     /locus_tag="VLL06_00105"
     CDS             6823..7563
                     /locus_tag="VLL06_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HSS29397.1"
                     /translation="MTTTIASRLQAVHARIAKVAQSIDRKPGEITLLAASKTNPAENL
                     REAWVAGQRIFGENYLQEALAKMQHLTDLPIEWHFIGPIQSNKTRRIAENFSWVHSVD
                     RTKIADRLSKDRSESQPPLQICLQVNVSGEASKSGVAPEELASLAAHVVRLPRLKLRG
                     LMAVPELTTATALQRSQFHMLWELFDRLKKDGYELDTLSMGMSEDLDNAIAEGATMVR
                     VGTAIFGPRRYLIPEEIAALAAKKAQGL"
     gene            complement(7669..>7957)
                     /locus_tag="VLL06_00110"
     CDS             complement(7669..>7957)
                     /locus_tag="VLL06_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29398.1"
                     /translation="GHFGEGLYRTVVALASPAQDVRVGPVAAGHSKGLGFWLALFTSI
                     VALSFFWWITRPDLRHPYKRIQKGEYWGTGQGGFGGNWGGFGGGTGGEGFR"
BASE COUNT         1946 a         2227 c         2136 g         1648 t
ORIGIN      
        1 gctgtaaccc tgacgcatcc cgactggcgt atttctgtct taacatatcc gatatcgcga
       61 ttccttcccc ccctcccttg acttgcgctc gacggaagct atctcctcat taactagaca
      121 tacttccgcg tcttttcacc ggccgagcga agggatgctc gacatgaaca acgagacact
      181 tgccgtcaag ctaccggttc tcccgatcaa acgcacggta ctgttccctg gaatcatgat
      241 gcccctcacg gtcgggcggg aacggtcgat cgctgccgta gaggcggcga tgaaaacgga
      301 ggataagacg attctcgtgg tcgcgcagcg cgatcccctg aaggacgcac cagggctcga
      361 agacctctgc acgatcggca ccaaggccat tgtgaaacag atcggtcaat cagacgaagg
      421 ggcgatccat gcgctcgtgc aggggctcga acgcgtcgcg ttgctcagag ccgagcagtc
      481 ggaaccgttt ctgcttgtcc aagccaggcg actcgagcgt ccggcggacc atggcacgga
      541 agttcaggcc ctccaccgcg ctatccagga attggtcaca gatcttccac gattggtaca
      601 ggctcccggc attcaggaag ccacagtggc cttcagtaac gaggacgatc ccatcgcgtt
      661 ggcctatcgc atcgcatcgc tcctcaatct gaccgtgcaa gacgagcaaa aactactcga
      721 aagttcgtcc acagttgagc tgctgcgatc gctgtacgga gcgctctcga aagaaattca
      781 aatcctacaa ttgagagaga agatcactag cgatgcgcaa accaacatcg ggaaatctca
      841 acgggaatat attctgcgtg aacaattgaa agcgattcat caggaactcg gcgagggcga
      901 aggagaggag aacgagatca ccgagctcaa aaagaagatc caagataccg atcttcctga
      961 ccatgtgcga aaggaggtgg agcgggagct cgctcgattg gcaaaggtgc caccctcttc
     1021 gcccgaccac caggtcctca ggacctatct tgagctggtg cttgagcttc catggaagaa
     1081 ggcttcggaa gaccggttgg agttgagcac cgtgcgtcag gttcttgaag aagaccacta
     1141 tggcattaag gaagtaaagg agcggatcgt cgagcatttg gctgtattga aattgaatcc
     1201 cacggccaag gcccccatcc tctgcctagt cggaccgccg ggcgtaggga agaccagtct
     1261 gggccagtcg attgccaggg cgatgggacg gaacttcgaa cggtttagcc tgggcggcat
     1321 gcacgacgaa gcggaattac gcggccatcg tcggacctac gtcggggctc tgcctggacg
     1381 gatcattcaa tccatgcgcc gagccggcgt caacaatcct gtcctgatgt tggacgaagt
     1441 cgacaagatg ggccgagatt tccggggcga tccggctgcg gctctcttgg aagtgttgga
     1501 tccggcacag aatcacacgt tccgggacca ctatctggat ctgccattcg atctctcgaa
     1561 ggtgttcttc attacgacag cgaacacact ggatacgatc agccagccgt tgctcgaccg
     1621 gatggaaatc atccggcttc agggttacag cgaacgggaa aaggcggaga tcgcccgtcg
     1681 ctacctctgg ccgcgacgac tcaaggaagc aggtctggag gccagtcaag cgctgctctc
     1741 agatgaagtc ttgaaccttg tcattagtcg ctataccagg gaagcaggtg ttcgtcagct
     1801 ggagcagatg ctcggccacc tcacccgtaa ggtggcgtta accttcgccg atcttccggt
     1861 tgaccaggaa cgacagcccg tcaccattca tgccgatctc gtcggagaat ggctcggctc
     1921 cgaacgcttc atgcaggagg aggctcgcaa agtcttgcct gccggcgtgg ccaccgggct
     1981 cgcctggact ccgagcggag gggatgtgct ctatattgaa acgactctgc tccccggcag
     2041 ccacgacctg acgatcaccg gtcagttggg agacgtgatg caagagtctg ccagagctgc
     2101 gcgcagctat ctctggtcgc acgcggaaag catgggactc gatatttccc gtttcaaacg
     2161 aaatggggta catattcacg tgccttccgg agcgattccc aaagacggcc cttcggccgg
     2221 aatcacgatg gccacggctc ttgcgtcctc gtatattgga gaaccggttc gtagcgacac
     2281 ggcgatgacc ggcgagatca gtctgagcgg attggtcctt ccggtcggcg ggatcaagga
     2341 caaagtgctg gccgctcatc gtgccggcat caaacgggtg attctgccca agtcgaacga
     2401 gaaggattta aaagaggtgc cgcaagaagt gcgcgacaat ttgaccttca ttctggtgga
     2461 acgcatcgaa gaggtcctcc ccgctgcctt caaccatgag acagcaactc acggaagctt
     2521 ccgcttcgga tcaggtattg ccccagcttc agcgtaaacg aagggattcc ggaacaacat
     2581 tccggaatcc ctcgtttctt ttcaatcatt ccagtgactt gcgtccagac ttctccatat
     2641 gtaaggatcg tttctgatct tgactcagct ggtagaggac agccgaataa gccaagaaga
     2701 aaggagcata accatgcccc caaaaattga gatcggcacg attgtcaaag tggtgaagac
     2761 cgacgaggaa tggaagaaaa tcttatcacc ggccgcctat cgtgtgttac ggcaggagga
     2821 taccgagcgg ccctttaccg acaacatgca cgataacaaa aaggccgggg tctattcctg
     2881 cgccggctgt gaccttccgg cctattcatc tgagcacaaa ttcgacagcg gcaccggctg
     2941 gcccagcttt tggcaaccga tcgatcccaa agtggtcgaa tccaccaccg actataagct
     3001 gatttttcca cggaccgaag tccactgcgc ccgctgtgag gggcatcaag gacatatctt
     3061 taaggatggc ccgagaccga cgggattacg ctactgcatc aatggcgtcg cgctcaagtt
     3121 tacccctgca tagcgacacc aaagcaggtg ccagccaccg cagaaactga aaaaaggact
     3181 tgcaaacaaa gaacatgatg ggtaccatcc gcgccgtggg taacgccaaa cggcgtgggc
     3241 tttcaaatgc ttcaacaccg ctcagattac ggctagaaag aattgcacac cacctcaatg
     3301 accaaggacc gcacctcatc aaaatacaac cgggaggagt cgcaatgaaa cgaccgagac
     3361 ccaataccga tctggcgcca tcaatccaat tccaacaacc agcttttgga gattcgcttc
     3421 aactgggcaa gcgcgtgctc ggatgcctgg ccgtctgcgc gatagcgatg ctgtcgtcct
     3481 ggatcgcggc accggccgcg gatgccgtgg tatcgaatga gtacaagttc aagcttcccc
     3541 ttggcctgga agagagtgcc atggtcattc catccgataa tcctgtgacg actcagaaag
     3601 tcgagttagg ccggatccta ttcttcgaca agcggttgtc gaatgacaac acgatcgcct
     3661 gtgcgagttg ccacctgatg caacacgggt ttacggacgg ccagccggtc tccacaggca
     3721 tccgaggcca gaagggcggc cgcagcgcgc cggcatcctt caaccgggtg ttcagccacg
     3781 ctcaattttg ggacggccgg gctcccgaat tggaagatca atcgatcggt ccctttacga
     3841 atccgatcga gcacggcttt gtcaattacg acgtcatggt tacgaagatg aagaagatca
     3901 aaggctacaa tacactcttc ttgcaagcct tcggcgaaga catctcgatt ggtctcgtgg
     3961 gcaaggccat cgccagcttc caacgcaccg tcctttccgg caacagtccg atggaccgat
     4021 ttgatcaggg cggagaagcc aatgccattt caacagaggc ccaacgcggc ctcctcctgt
     4081 tccgagacaa agcccgctgc acgaagtgcc attcggggtt caacctcacc gacgaaaagt
     4141 ttcacaacct cggcatcggc tgggacgaca acaaagtgga tctcggtcgg tatatggtca
     4201 ccaagaacgc ggaagaactc ggcgcattta agactccgac tttgcgggag atcgcccgta
     4261 ccgcccccta tatgcatgac ggccgcttca agaccctcga ggatgtcgtg aacttttaca
     4321 accaaggcgg agtgaagaat ccacaccagg acccgttgat ccttccactc gaattgacga
     4381 aggaagagaa gcatgatctt gtgcagttcc ttcgcacgct gaatggagag gggtacaagc
     4441 acgccacagc gccgaccagc tttcctcagt aggaaaagga acgcagcgtc actcgctttg
     4501 aaataggacg agaaaatgcc aaggcggcgg ttccgactat aacaatcggg ccgccgcttt
     4561 ggtgcttttg gttatattga tcgagggaac gtggaccgga tggtcagggt agctttcggc
     4621 tatgcggcag gacgatgacg ccgacaacct cgttcagaac ctgctcccgg atgaatttca
     4681 gtacatgaac agggagcagg tagatggctc ctcaccacat gatttttgcc gtgtcgaacc
     4741 ttcatgtgaa ccggttaacg ggattcttca atcacttcac tttcaccaac catccaccgc
     4801 tggcatcgca ttggttgggg cctttcgccg caacgtttat ttttgcgaag gttcctttta
     4861 cagcgtcagg gagtttgccg gttacttgaa acccatcatg tttttcggtc actgtaaccg
     4921 gcaccaaatg gtcttttatc gtcactctaa tgctggtcgg gttggtcgaa tgggatgcaa
     4981 aaaatgaaaa ttcagatttt ggcgccacct ctgaattatt tgccggcgaa aatttcgaca
     5041 acttggccgt gcacgcgata agcccactgc caccaccact ctcaccgtag gcccaaactg
     5101 tgcctgggat catcaagacg atcgctacca agaaaatgtt ccgcactttc atagtgatgc
     5161 ctctcctgtt attagtgagt cggttggctg gttgagtacg actggcccat gaacggaatc
     5221 cattgtcagt tactttttta caaaaaacaa cccccgcgcg attgagctaa ttcatacgat
     5281 tcttcgctcg tcggacggca gccgcatggc tatcacattc tcataaccgc gatgagatcc
     5341 gcccctccct gttgaaaacc atgcgccagc atgacagccg ggcctctttg ttggttttcc
     5401 cgcccattgc atcttgacaa gaaccaggga aagagctaat cacattgacc cgttggggat
     5461 tctcaagggt gcttgagcct cccccacttc aaaagcaacg tcctaattac ccggaagagg
     5521 atggtcatgc taaaaactct cgaacaactt ttcgacaaat tgagcgatca ttcgccggat
     5581 gtgtgttttg aggtgcggtt ctgggacggg aagaaaaagt cctacggtac gggtgagagg
     5641 cagttccttc tcacctttgc gacaaagcga gcagcagagc atgtgattga aagcggatcg
     5701 ttaggattcg gcgaagagta tatgtcggga catattcaag tagaggggga tttcagacaa
     5761 cttcttcgct tcgggacaga cgctgcgttc ctcaatctac ctctcgggtt gaaaacgaag
     5821 ctcgcatttc agcacctgcg tcagacctca ctcaacacgc tctcgcgtgc cccaaaacac
     5881 atcgcgcacc attacgatct cgggaatgat ttctacaagc tgtggctcga cgacagcatg
     5941 gcctactcct gtgcctactt ccgtcatgaa cacgacacgc tcgaggaagc acagcagcaa
     6001 aagtacgaac acatttgccg gaaacttcag ttgaagaccg gagaaacgct ggtggacatc
     6061 ggctgcggct ggggagggat gctgagatac gcagccaagc actatggcgt gcgcggagtc
     6121 ggttgctcac tgtcgaagca gcaggttgag tacgcgaccg atcttgtgac acgcgagggg
     6181 ctgtcagata aggtttcggt cgtgttcgaa gattaccgca agcttgaggg ccagttcgac
     6241 aaattcgtgt ccatcggcat gtttgaacat gtggggaagc aattcattcc gacgtttatg
     6301 gaaaaggcca ggtcgctctt gaagcgcgac ggcatcgggc tgctccacac catcggagaa
     6361 gagcgatcga tgccaggcga tccctggacg ttgaaatata tcttcccagg tggatacatt
     6421 ccctcgctcg atgaaatcgt tcgtaccatg ggagaaaccg gactggttcc gacggacatc
     6481 gaaaacctcc gcctgcacta tgccaaaacg gttgaggaat ggtgcacgcg attcgaggca
     6541 caaaccaaaa agattgagac gatgttcgat gcgtcgttcg ttcgcatgtg gcgaatgttt
     6601 ctcaatggat gtgttgtcaa ttttcgctat ggaaacccgc gactctatca ggttcttttc
     6661 accaacggat tgaacaacac ccttccactt acccgtgact atatgtatcg ataaaccgct
     6721 tctggaacca cttcagtaca acattgaaat atacatgaag acgtatgctc tccatgttgc
     6781 tttcagagat gagtcaattc aggacaagga acagccctac acatgacgac aaccatcgcc
     6841 tctcgcttgc aggccgtgca tgcccgaatc gccaaagtgg cgcagtctat cgaccgaaaa
     6901 cctggcgaga tcactctgct ggcagcgagt aagaccaacc cggccgagaa cttgagggaa
     6961 gcctgggtcg ccggacaaag aatcttcggt gagaattatc tgcaggaggc attggcaaaa
     7021 atgcagcatt tgaccgattt gccgatcgaa tggcatttca tcggtccgat acaaagcaat
     7081 aagaccaggc gcattgcaga gaatttctcc tgggttcaca gtgtggacag aacaaagatc
     7141 gccgatcgcc tgtcgaaaga ccggtccgaa tcacagccac cgctgcaaat ctgtttacaa
     7201 gtcaacgtga gcggagaagc gagtaagagc ggtgtggcac ctgaagagct ggccagtctg
     7261 gccgcgcacg tcgttcgcct gccccgccta aaattgcgcg gattgatggc cgtccctgag
     7321 ctcacgaccg ccacggcctt gcaacgcagc cagttccata tgctgtggga actgttcgat
     7381 cggctcaaaa aagatggata cgaactcgac acactctcca tggggatgtc agaagatctg
     7441 gataatgcca tcgccgaagg cgcaacaatg gtgcgcgtag gtacggcaat tttcggacca
     7501 cggcgctatc tgatccccga ggaaatcgcc gccctcgcag ccaaaaaagc acaaggactc
     7561 tgattggctg agggtccgcc acccattgcc aggccgagga gcaccattcc ccaggcgaag
     7621 accttccctc agcgcgcgtc ccgcgagcag gaggatgacc aggccatact accgaaatcc
     7681 ttcgccaccc gtcccccctc cgaacccacc ccagttcccg ccgaacccgc cttggccagt
     7741 tccccagtac tctccctttt gaattcgttt gtagggatgc cgaaggtcgg ggcgagtgat
     7801 ccaccagaag aacgagagtg ccacgatgct ggtgaagagt gcgagccaga aacccaggcc
     7861 tttggaatgt cccgctgcga cggggccgac ccgcacgtcc tgcgcgggag aggccagcgc
     7921 caccacagtc cgatagagac cttccccgaa atgtccc
//
LOCUS       DATIPF010000004         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1150210, whole genome shotgun sequence.
ACCESSION   DATIPF010000004 DATIPF010000000
VERSION     DATIPF010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150210"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VLL06_00115"
     CDS             complement(<1..406)
                     /locus_tag="VLL06_00115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSS29399.1"
                     /translation="MSGTIQWSIVLPAYNEAARILPYLRSITSFMQDRGQPYEVLVVD
                     DGSTDSTAAIVESLASSTPEIQLLRAPVRRGKGAAVRRGMHAAVGRLQLFADADGATP
                     IQELARLEQAVANGADMAIGSRALASQLPDYAV"
     gene            887..2404
                     /locus_tag="VLL06_00120"
     CDS             887..2404
                     /locus_tag="VLL06_00120"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HSS29400.1"
                     /translation="MVIRSKEGNIRGLVAGIGLVMGTFACTTLPNEPSAIAAGVPAGL
                     AQGFSEIVKQVTPAVVNIAVTGGGKSGGRGRRPPLPPGPFEGPPGEEAPGGELPTPPP
                     MPPGGGHGRPDQSAGSGVIVDASGYIVTNNHVVEGATQITVTLSDRREFSAKVVGVDP
                     KTDLAVVKIEAKNLPALKWAEYEKLQVGDVVLAVGSPFGLSSTVTLGIISALGRGNVG
                     IADYEDFIQTDAAINPGNSGGALVNLNGELIGINTAIFSRTGGSEGIGFAIPSSIALD
                     IVESLQKTGKVVRGWMGVAIQEITPALAKSFKLPEQRKGVLISDVNENGPSHAAGIKR
                     GDVVIAFNGKEVLNVSQLRNLVARTMVGKDAQVKVLRDGKEQTIAVKVAERPTDEMLA
                     KKEPSPPKDPVETVKLPDNVLASLRVQALDAATMSQLNIPAKMTGVVVTSVENGGPAE
                     AAGLQRGDVIQEVNHEVVKTLEDYQKASSVLKKDELAVLLLSRQGNSLFVAVNPQ"
     gene            2475..>2878
                     /locus_tag="VLL06_00125"
     CDS             2475..>2878
                     /locus_tag="VLL06_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HSS29401.1"
                     /translation="MSYGLNSINQWLQDTIFKPLEDKKMPVMEHLVELQVRLTRVAII
                     TAVVFVGTFFYADQLVKWLRIPLQNMFMPGSLSWAPTDLPTVPFVFLAPAEALWQNVK
                     VAGLFALGAATPYILWEVWHFVVPGLHAQERR"
BASE COUNT          623 a          714 c          929 g          612 t
ORIGIN      
        1 ggacggcata gtctggcagc tgtgaggcga gtgcgcgcga tccgatagcc atgtcggctc
       61 cattcgccac ggcctgttcg agccgggcga gttcctgaat cggagtcgca ccgtccgcat
      121 cggcaaagag ttgtagccgt ccgacagcgg cgtgcatccc tcggcgtacg gccgcgcctt
      181 tccctcggcg cactggtgca cgaagcagct gaatctccgg ggttgatgaa gcgagcgact
      241 caacaatggc ggccgtggaa tcggtgctgc catcgtcaac cacgagcact tcgtaaggct
      301 gtcctcgatc ttgcataaat gaggtgatgc ttcgaaggta gggaagaata cgagcagcct
      361 cgttataggc aggaagaacg atggaccatt ggatagtgcc tgacatattt tgacaggata
      421 ggggaaccaa tcatgtactg gtgatgggcg atagtgcccg gctcagagct ttattcgagc
      481 atggtagcga gcctcaagat tgattgcaag ggtgggggat gctactggac ttgacagacc
      541 agccactggg gagcgaacgt gggagtgtga agcagcggat gacttagtcc catgttcagc
      601 gcaggtattc cagcgacccc cgcaggctgg tgtcagatat ggacgagcga gggatgctga
      661 acgagcgaga cgggcacacg tgataggagc ctaatctgtc cccatcgcac cctttttgcc
      721 tcgcgcgcgc aacatgatgg tcatgtgtat tggcttccct tcaggctcat ctgaataaag
      781 gctgggcggg ggactattag tctttgaagc cctcggaact gatagtgtac aatgttgcgt
      841 tcagcttatg ctgtcgtatt gattaaccct gtggaggaac gctgttatgg tcatccggag
      901 taaggagggg aatatccgag gtcttgtcgc aggtatcggt ttggtcatgg gtacctttgc
      961 atgtactacc ctgccgaatg aaccctcagc catagccgcc ggggttccag ccgggctagc
     1021 acaaggattt tcagaaatcg tcaagcaggt tacgccggcg gtggtcaata tcgcggtaac
     1081 cggaggtggg aaaagcggag ggcgaggtcg gagaccgccg ttaccgccag gtccttttga
     1141 gggacctccg ggtgaagagg cgccgggggg tgaactcccg actcctccgc cgatgcctcc
     1201 gggtggaggg catggacgcc ctgatcaaag cgccggatcc ggcgtcattg tggatgcgag
     1261 tggatatatc gtaacgaaca atcacgtggt agaaggcgcc acgcagatca ccgtcacgct
     1321 gagcgatcga cgtgaattca gcgcaaaagt ggtgggagtt gatccgaaga cggacttggc
     1381 tgtggtcaag atcgaggcca agaaccttcc ggccctaaag tgggccgagt atgagaaatt
     1441 gcaggtcggc gatgtggtat tggcagtcgg tagtccgttc ggactgagtt cgaccgtgac
     1501 cctgggcatc atcagtgcgc taggacgcgg caatgtcggc atagccgact acgaagattt
     1561 cattcagacc gatgccgcta taaatccggg taattccggc ggggcgctgg tcaatttgaa
     1621 tggcgaactc attgggatca atacggcaat tttttcaagg accggcgggt cggagggaat
     1681 tggatttgcc attccgagca gtatcgcgtt ggacatcgtc gagagcctgc agaaaaccgg
     1741 taaggtggtg cgcggttgga tgggggtggc gattcaagag atcacgccgg ccttggccaa
     1801 atccttcaag ctcccggaac agcgcaaagg cgtactcatc agcgatgtga atgagaacgg
     1861 cccttcccat gcggcaggta tcaagcgcgg cgatgtggtg attgcgttca acgggaagga
     1921 agtcctcaat gtgagccagt tgcgaaacct tgttgcacgt acgatggttg gcaaggacgc
     1981 gcaggtgaag gtgttgcgag acggtaagga gcagaccatc gccgtgaagg tggcggagcg
     2041 gccgacggat gagatgttgg caaaaaagga gccgtcacct ccgaaggacc cggtcgagac
     2101 cgtgaagctg ccggataatg tcttggcgtc tcttcgcgtg caggcgttgg atgcggccac
     2161 gatgagtcaa ttgaacatcc cggcgaagat gacgggtgtg gtcgtgacgt cggtcgaaaa
     2221 cggtggtcct gcggaagcag cgggtctgca gcgcggtgac gtgattcagg aagtgaatca
     2281 cgaagtcgtg aagacgctcg aggattatca gaaagcttcg agtgtcctga aaaaagatga
     2341 gttggctgtt ctgctcctga gtcggcaggg gaacagcctg tttgttgcgg tcaatcccca
     2401 gtaatacgca tgacgctggc cgcggccgct cggcatccga gcagccgcgg ccaaccaccg
     2461 gatgaatggt tcgaatgtcc tacggcctga attccatcaa ccagtggtta caagacacga
     2521 tcttcaaacc gctggaagat aaaaaaatgc cggtgatgga gcacctcgtg gagcttcagg
     2581 tccggttgac acgcgtggcg atcatcacgg ccgtggtgtt tgtcggcaca tttttctatg
     2641 ccgaccagtt ggtcaagtgg cttcgcattc ctctccagaa catgtttatg ccaggctccc
     2701 tgtcgtgggc gccgaccgat ctgccaacgg ttccattcgt ctttctcgcg ccggctgagg
     2761 cgttatggca aaacgtcaag gtggctgggc tgtttgccct tggtgctgcg acgccgtaca
     2821 ttctctggga agtctggcat ttcgttgtgc cgggtctcca tgcccaggaa cggcgttt
//
LOCUS       DATIPF010000005         4684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_708153, whole genome shotgun sequence.
ACCESSION   DATIPF010000005 DATIPF010000000
VERSION     DATIPF010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708153"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..2085)
                     /locus_tag="VLL06_00130"
     CDS             complement(139..2085)
                     /locus_tag="VLL06_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HSS29402.1"
                     /translation="MLTSKPRPFLTRLWNIFLLLLVLFLAPVLPVDASEPSENVYLAE
                     LINRGLQAKLASEREWHLLLHYRKDLFGGDTSEQDDPGFFMSPNGKTDPQAELDATLK
                     QFFSNELVGRSKQPAQCAFIARYEWLRNQLHFDATRLPPMACERFDRWFADFEAQSIT
                     LVFPSAFLNNPASMFGHTLLRVDQKGQTEQTRILAYTINYAADVPPDAGMAYPIRGIF
                     GGYRGYFSTIPYYLKVQEYRDIENRDIWEYRLNFTDDQVRRLLMHAWELGNASFDYFF
                     FKENCSYHLLALLDYADPTLHLTDEFLLWTVPADTVRLVASKPGLVSGIAYRPSRSNV
                     IRRKRESLPPDERAIAQRLTQDVGELRSAALNRLEPMRQTFLLDLASDYLRYRIDTTD
                     EPPPILKERNRAILAARSAIRLPSEEFRVEPFAKQPELGHNTSRISVGAGWRNNSAFE
                     EQTIRAGYHDLLDPEIGYTPNAQIEIASVTVRTYNQHNQTRLERATLANVVSLSPMDS
                     VFHAPSWKINVGMNTIKHGGCQLCSNGVMNGGIGAAVESRWLGHQVFFAFAEAEANYS
                     RAYDERHRIGGGGSVGMLADLTERWKLLASGTYLRYPLGDKSDDFRWTVGQRLTLLQN
                     LTLRLDYNHRDHDNDVMFSMQAFF"
     gene            complement(2105..2614)
                     /locus_tag="VLL06_00135"
     CDS             complement(2105..2614)
                     /locus_tag="VLL06_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HSS29403.1"
                     /translation="MSKKVIMLSVAVLFGMQAGLAMAANPDTGPGCGLGKLAWSDYSH
                     QKNIGPQVLMATTNGTFGSQTFGISTGTSGCTNDGKVMAEQKTTMFAQLNFENLSQEM
                     AQGQGEHLASLATLMGVPVEHQAAFFAMTQERYTSLVKTGESSPVAMVKAINDAIATH
                     PVLAQVSAR"
     gene            complement(2753..3268)
                     /locus_tag="VLL06_00140"
     CDS             complement(2753..3268)
                     /locus_tag="VLL06_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HSS29404.1"
                     /translation="MKVWKIVTVIALASISQMGIAMAYGNPDTGPGCGLGKLLWSDFK
                     RQKDIAPQVLMATTNGTFGSQTFGLSTGTSGCTNDGKVWAAQKTEFFVAATFENLAGD
                     MARGQGEHLTALATLLGVPTDQQEMFATLVQERYRELISRGESSPSALLKALDEAMAG
                     YPVVAKVEAGH"
     gene            complement(3378..3890)
                     /locus_tag="VLL06_00145"
     CDS             complement(3378..3890)
                     /locus_tag="VLL06_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HSS29405.1"
                     /translation="MNQWNTIRIGVIADTHGLFDPVVRQDFTGVDHIVHAGDIGDRSV
                     IEQLELLAPVTAVSGNVDGYEQSGFPSEVVIEVAGRRIAIRHILYEGGKMTKEGREFL
                     EREHPDICIFGHTHQSRAEWFGKTLLFNPGSAGPKRFTLPRGIGLLRLRDAEVEPHLI
                     SLPDKPHRSS"
     gene            complement(3887..>4684)
                     /locus_tag="VLL06_00150"
     CDS             complement(3887..>4684)
                     /locus_tag="VLL06_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HSS29406.1"
                     /translation="RLVWGVGGGVILSTVLGLWFGADVLVEIAVSRIPVEWEQKLGES
                     AYRDFLTHQEVMKDGPAVAALGEITQRLTEQISNNPYKFDVTLVKSDVVNAFALPGGY
                     VVVFTGLMKKAESGEEVAGVMSHELNHVLQRHGLERIVKNLGLMTVVAIVFGNQQGLV
                     GMMKQFGVELLTLKFGREQETEADLTGLQLLQRAKIDPSGMIRFFERLSEKDEGRMEW
                     LSTHPMSTARAERLKTELAALPKKLPEPFTFNWKQVQASLGSQSGAP"
BASE COUNT         1043 a         1336 c         1250 g         1055 t
ORIGIN      
        1 aaagccgtga tcgaagctca agaattacaa gataaattca ttgagcagaa acaaaaatct
       61 acatccgcac ctacagagcg agtgaactcg cctcttgctc agtaaaggga agctgcctgg
      121 ttgctgctag gattctagct agaaaaatgc ttgcatcgag aacatgacgt cgttgtcgtg
      181 atcgcgatgg ttgtagtcga ggcggagtgt cagattctgg agcaatgtga gccgctggcc
      241 gacggtccaa cggaagtcat cactcttgtc ccccaaggga tagcggagat aggtccccga
      301 cgcgagcagt ttccagcgct ccgtgagatc cgcaagcatg ccgaccgacc cgcccccgcc
      361 gattcgatgg cgctcgtcat aggcgcggct atagttcgcc tcggcctcgg cgaaggcaaa
      421 aaagacttgg tgtccaagcc agcgactttc cactgccgct ccgatcccgc cgttcatcac
      481 tccattgctg cataactgac atcctccatg cttgatcgtg ttcatcccga cattgatctt
      541 ccacgacggg gcgtgaaaca ccgaatccat cggagagagc gagacgacat tcgccagcgt
      601 ggcgcgctcc aaccttgttt gattgtgctg gttataggtc cgcaccgtca ccgaagcgat
      661 ctcgatctgg gcattcggcg tgtacccgat ctcgggatcg agcaggtcat gatatcccgc
      721 gcgtatcgtc tgctcttcaa acgcactgtt gtttcgccag cctgcaccga cactgattcg
      781 tgaggtgttg tgacccagtt ccggctgttt cgcgaacggt tcaacccgaa actcttccga
      841 gggcaatcga atggcgcttc gggcagcaag gatggcgcgg ttccgctctt tcagtatggg
      901 cggtggttcg tcggtggtat cgattcgata ccggaggtag tcggaggcga ggtcgagaag
      961 aaaggtttgt cgcatcggtt ccaatcgatt caatgccgcg gagcgcagct caccgacatc
     1021 ctgtgtaagc cgctgggcga ttgctcgttc atccggaggc agggattcgc gcttgcgccg
     1081 gatcacattg ctgcgggaag gccgataggc aataccagag accagacccg gtttcgatgc
     1141 gaccagtcgg actgtgtcgg caggaacggt ccacagaaga aactcgtcgg tgagatgcag
     1201 cgtgggatcg gcataatcga gaagtgccag gagatgatag gagcagttct ctttgaagaa
     1261 gaaatagtca aaggaggcat tcccaagttc ccacgcgtgc atcagcaacc gcctgacctg
     1321 atcgtcggtg aaattgaggc ggtattccca aatatcacga ttttcgatgt cgcggtattc
     1381 ctgcaccttc aagtaatagg gaatcgtgga aaagtatccg cgataccccc cgaaaatgcc
     1441 gcggatcgga taggccatac cggcgtccgg aggcacgtcc gccgcataat tgatcgtgta
     1501 cgccagaatt ctggtctgtt cggtctggcc cttctgatcc actcgaagca acgtatgccc
     1561 gaacatcgac gcggggttgt tcaggaacgc ggagggaaac acgagcgtaa tcgattgggc
     1621 ttcaaaatcg gcgaaccagc ggtcgaaccg ttcgcaggcc atcggaggga ggcgagtcgc
     1681 gtcgaagtgc aattgattcc gtaaccattc ataccgcgca atgaacgcac attgggccgg
     1741 ttgcttcgag cgcccaacaa gttcattgga aaagaactgc ttcagcgtcg catcgagttc
     1801 tgcctgtgga tcggtttttc cattgggcga catgaagaat ccgggatcgt cctgctcgct
     1861 ggtgtcgcca ccaaacagat cttttcggta gtggagcaga agatgccact ctcgctcgct
     1921 ggcgagtttg gcctgcagac ccctgttgat cagttcagca agatagacat tttcggaagg
     1981 ctcactggca tcaaccggca agactggagc gagaaatagc acgagaagaa ggagaaagat
     2041 attccagagg cgggtgagaa agggacgggg cttcgatgtc aacatcgaag ccccgtgctg
     2101 gtgattaacg agccgaaact tgggcaagga ctggatgcgt ggcaattgcg tcgttgatcg
     2161 ctttgaccat ggcgacgggt gaggattcac ctgtcttgac caaggaggtg tagcgctcct
     2221 gagtcatggc gaaaaacgcc gcttggtgct cgaccggcac acccatcaag gtggcgagcg
     2281 atgcgagatg ctcgccctgg ccctgtgcca tttcctgaga caaattctca aagttgagct
     2341 gcgcaaacat ggtggtcttc tgctcggcca tgaccttccc atcgttcgtg cagcctgagg
     2401 tccccgtgct gattccgaat gtctggctgc cgaacgtgcc gttcgtggtc gccatcaaca
     2461 cctggggtcc gatgttcttc tggtgcgaat aatcgctcca ggcgagcttg cccagcccgc
     2521 agccagggcc tgtgtctggg ttagcggcca ttgcgaggcc tgcctgcatg ccaaagagca
     2581 ccgcaaccga tagcatgatc acctttttcg acataagtcc tcctccttta gatagagttg
     2641 gttagaagcc actgcaacta tacgaaacac cacgtcgcag tatagcccaa ctcagcacaa
     2701 ttgtttacaa aaaaatactt tgcctctcga ggatcctacg gacttgtgcg tattaatgcc
     2761 ctgcttcaac tttggccaca accggatatc ctgccatcgc ctcatcaagt gccttgagga
     2821 gtgcactcgg tgaggactca ccacggctaa tgagctcgcg ataacgttct tgcaccagcg
     2881 tagcaaacat ctcctgctgg tctgtcggca cgccgagtaa cgtcgccaac gccgtcagat
     2941 gttcgccttg gccgcgcgcc atatccccag ccagattctc aaacgtcgca gccacgaaga
     3001 actctgtttt ctgtgctgcc cagactttcc catcatttgt gcagccggat gttcctgtgc
     3061 tgagtccgaa ggtctgactt ccaaaggttc cattcgtcgt cgccatgagg acctggggag
     3121 cgatgtcttt ttgccgttta aaatctgacc agagaagttt gcccaatcca cagccggggc
     3181 ctgtgtcggg atttccatag gccatcgcaa tccccatttg gctgatactc gcaagagcta
     3241 tcaccgtcac gatcttccat accttcatca ccgatacctc cttgtgcttc ttcgcgcatg
     3301 catgtttcag aatgctgctt acctatacat agcggaactg atgatccctg ccaagacaat
     3361 tatccccgct gtttctatta cgaactacga tgcggtttat caggcaaaga aatgagatgc
     3421 ggttcgactt cggcgtcacg aagtcgcaga agaccgatgc cccgaggaag cgtgaatcgt
     3481 ttcggtcccg cggatcctgg attgaatagc agtgtcttac cgaaccattc tgctcgtgac
     3541 tgatgcgtgt gaccgaagat acagatatcg ggatgttcac gttcaagaaa ttcgcggcct
     3601 tctttcgtca tcttcccccc ttcatacaga atatgacgaa tggcgatgcg gcggcctgcc
     3661 acttcgatca cgacttcgga cggaaatcca ctctgctcgt agccatccac gttgccggag
     3721 acggcggtga ccggagcgag cagctccagc tgttcgatca cagaccgatc gccaatgtct
     3781 ccggcgtgga caatgtgatc gacgcctgtg aaatcttgtc gcaccaccgg atcgaagagg
     3841 ccatgggtgt cggcaatcac tccgatccga atggtgttcc actggttcat ggggcaccag
     3901 actgggatcc gagtgaggcc tgcacctgtt tccagttgaa tgtaaatggc tccggcaatt
     3961 ttttcggcaa ggcggcaagc tccgtcttca atcgttcggc tctggcggtg ctcatcggat
     4021 gcgtcgaaag ccattccatc cgaccctcgt ctttctccga gagccgttcg aaaaatcgga
     4081 tcataccgga aggatcgatc ttcgcccgct gaagcaattg cagtcccgtc agatcggctt
     4141 cggtttcctg ctcccggccg aacttaagag tcaggagttc gacaccgaac tgcttcatca
     4201 ttccaacgag cccctgttga ttaccgaata caatggccac caccgtcatc agcccaaggt
     4261 ttttcacgat acgttcaagc ccatgccgct gcagtacgtg attcagctca tggctcatca
     4321 cgcccgccac ttcttcgcca ctctctgcct ttttcatgag tccggtgaat accaccacat
     4381 acccgccggg gagcgcgaaa gcattgacga cgtcactctt caccagcgtc acgtcaaact
     4441 tgtagggatt attcgaaatc tgttcggtca gacgttgcgt gatttctccc agcgctgcga
     4501 ccgctggacc atccttcatc acctcttgat gggtgagaaa gtcccgatag gccgattccc
     4561 ccaatttctg ttcccactcc accggaatgc ggctcacggc aatctcgacc aagacatcgg
     4621 ctccgaacca aaggcccagt accgtagaca gaatcacccc gccgcccacg ccccagacga
     4681 gccg
//
LOCUS       DATIPF010000006         7907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1327594, whole genome shotgun sequence.
ACCESSION   DATIPF010000006 DATIPF010000000
VERSION     DATIPF010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7907
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327594"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VLL06_00155"
     CDS             complement(<1..636)
                     /locus_tag="VLL06_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29407.1"
                     /translation="MALIVNNNPASIAAQRNLTVNTNSLQRSVERLSSGLRITRAADD
                     AAGLGVSETLRAHIRSINQATRNANDGISLTQVADGAAATIGNLLSRLRELSSQSASG
                     TVGVTERSYLDQEFVSLRSEIDRIATVTEFNGQALLSGASNSFEVFIGFKSGTGNSLS
                     ADLNDLDITSIGLVPADGLDPAFAAVLPDAGLVPFHSISVAADVLRPGSTAV"
     gene            complement(862..1392)
                     /locus_tag="VLL06_00160"
     CDS             complement(862..1392)
                     /locus_tag="VLL06_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29408.1"
                     /translation="MRITLDHDQWDVSDNQPVSEVLTQVSDRAYARHRLVTSLKFGGR
                     PITDRDLQPLLLNKRLDEVGPIEACSQNIPEIMTNAAPAITRFASTLRADAQGFLIPL
                     RTSGVIPSALDRWFGQLADYVELLQAAGQGSLSGGNLRALSSWIQELLDARSVHDSVR
                     MADILEFEVLPYLASI"
     gene            complement(1649..3316)
                     /locus_tag="VLL06_00165"
     CDS             complement(1649..3316)
                     /locus_tag="VLL06_00165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HSS29409.1"
                     /translation="MKKQVLIINVTRMGDLIQTGPLLSRLRQEWPDVAIDLVVDRSFA
                     PTAALLTGLRQVISCDFTRLLDDCRTQSKSLVTLMQEMTTGAAPLRDARYNRIVNLTF
                     NRQTGLLASYIGAPDLRGITAGPDGNPIVHNPWLSYFTDLHRHRRFNRFNLVDLYAMG
                     GSGPGTFSPLSVTVPADGRDWADEFLCSQTTTVRQWIAVQVGASDVMKAWRPEYFGRT
                     LAVLSRHPGLGFVLIGTAAESESIEQAKATYRAGGGTAPMLDASGRTTLHQLVGLLSR
                     CRLLLTNDTGPMHIAVGVGTPVIDLSVGHVDHHETGPYGPGHWVIQPDLACSPCGFDQ
                     ICAHHACKDRLIPEEVAKLCLHTLGLGPFPSWSTGLRVYQSGADADGLGSFVMRAGHV
                     DSDTAWYAAYWRRFWYQDFTGKPSQLPPNLEAIPNQASALAWMKEAVHPLRRLEQRAM
                     HIVGLTKRHPLPVATLQREQALLRQEREQLLPRMMAYSATGPATVAMIRDIHNDDAVD
                     LTMLADRHVQAYRRWQQRIAQTRTLLSAGPVQTEARRCVLPMMPSAV"
     gene            complement(3422..5236)
                     /locus_tag="VLL06_00170"
     CDS             complement(3422..5236)
                     /locus_tag="VLL06_00170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSS29410.1"
                     /translation="MNSFEANVSRLAQRDSALAAALGESGGGTLEIMPARTGVPSARA
                     HGRWIHSAYDPIKEAQVWAESQSYQDGETIVVVGVGLLYHIEALCARLPRTVPVVLVV
                     PDLSVLADAAAARTWGNWMDRVSWAWGTTGVISEQISRAGHPLRLVTYTPAASLHADC
                     HRAIETALQRQVAAQAGGQLRIAVVGPIYGGSLPTTGFVVRALESLGHHVRWLDHSIH
                     ATSYHQLELLKNSQHRLALQSKYAEMLSRITMAQLAEDPPDLVLSLAQAPLILPMLEH
                     LRRKRFLTAMWFVENYRHLTYWQQLAAGYDFWFVIQQEPCIAALKQAGAKDVRYLPMA
                     ADPSVHRPLELTQAEQEEYGSDVSFVGAGYANRRAIFPRLLKREWRFKLWGNEWDGAT
                     DLAPVLQRHGTRIDTDTCQKVFNASAVNLNLHSWAGAGLDPDGDFVNPRTFELASCGA
                     FQLTDRRSLMPDLFTSSEIATVSSPDELPGEISRWLHEPEQRLAMAGQARQRVLTEHT
                     YAHRMRDLLSQIGVASPDRIGSILQGGRQAAALASTAADSFPSLAATLRTFPAGQRVE
                     LKQLAAQIRSRGPMGTLSREDLMVLMLDEYRSETKDLV"
     gene            complement(5236..6423)
                     /locus_tag="VLL06_00175"
     CDS             complement(5236..6423)
                     /locus_tag="VLL06_00175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HSS29411.1"
                     /translation="MTPHALMIQLARLGDLVQSLPAITSLSARYPDRSLDMLCPAPLA
                     DLARLFPDVGQVLEWNGAQWHAWAESFIGEFQPSWLRDVERHLAGLTTEPYAMAYVLN
                     HHPRAILAGTLLATEVQGPHLRGPLDEELSPWATYLRHVAQARGSNRIHLSDTFCGLC
                     GVTPPRVPPVIQLPSIDLPGDLAEIGILDGQWIAVVVGAGDADRSIPITVWIEWISTV
                     LASESPCAVVLVGAARDQAAAMAIQDGLSPMIAGRVWDATGRTTLWQLATLLKRCHWA
                     VGADTGPLHLATAVGTQALGFYFSRARVHETGPYGQGHWAWQADRAMPKTWPIRSSVA
                     LLREPASTWQQEPVAGWTLWQSHHDEWGAIFRPVGEEYAQDDQRAAVWHQLWEQHRLL
                     QDV"
     gene            complement(6430..>7907)
                     /locus_tag="VLL06_00180"
     CDS             complement(6430..>7907)
                     /locus_tag="VLL06_00180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSS29412.1"
                     /translation="FLAQLSGDIQIIRNSENLGFARACNQGARAARGRFLIFLNNDTI
                     PLSDWLTALVAEVTSHPEVGIVGSKLLYEDDTIQHAGVVFSREALMPYHLYRRVHRDH
                     PAVNQRRTFQSVTAACMLIRREIFEAEGGFDEQFQNGFEDIDLCLKVGDKGWQIVYQP
                     LSVLYHLESQTPGRKAHDHDNALRFLSRWGQRWWLADEDLYYYEDGYLAVHTKDQDKE
                     QLELRPLRGDQEKASWKLVADTERAAFRQDITQVESLLSRWDEWPSVADVLGWGAWLC
                     TKTSHASLSRQFYQKLVQHHDDPTARRVLARMALEEGNLSEADSHLDVLLKQFPQDGE
                     GWVLRGILDMQQAHYAEAEQAFLTALTHQGDRRKSMMGLGMARVGQGEIDRAWNDFST
                     VLNEYPDDAEAIHWLLRAGSARGLWTELSERLQGFVLRNPADLSVRYALAGVLIRSGQ
                     TEAAQQEYQTLCTLAPSYGGLEELARALSPHDTLIEFVPQS"
BASE COUNT         1715 a         2345 c         2247 g         1600 t
ORIGIN      
        1 cacggccgtc gacccgggcc tgagcacgtc cgcggccacg ctgatcgagt ggaatggaac
       61 cagtccggcg tctggcagca ccgcggcgaa cgcggggtcc aggccgtcag cgggaaccaa
      121 gccgatgctt gtgatatcca aatcgttcag atcggctgaa agtgaattgc ctgtgccgct
      181 cttgaagccg atgaagactt cgaatgaatt gctggctccg ctcaacagcg cctggccgtt
      241 gaactcggtc acggtcgcga tccggtcgat ttcagaccga agcgacacaa actcttgatc
      301 caagtacgac cgttccgtga caccgactgt accgctggca gactgcgatg acagctcacg
      361 aagacgggag agcaagttcc cgatcgtcgc agcagctccg tcggcaacct gcgttaagct
      421 gataccgtca ttcgcattac gcgtcgcttg attgatactg cgaatgtgtg cgcgcaacgt
      481 ttcagacacg cccaatccgg cggcatcgtc agccgcacgg gtaatacgca ggcctgaaga
      541 caaccgttcc accgaccgtt gcaacgagtt cgtattgacg gtcaaattgc gctgagccgc
      601 aatcgaagcc gggttgttat taacaattag agccatggac ctattcctcc ttgaagaccg
      661 atcacatcaa ccacttgcat cccccggcat ccgtgccagg attgttttga ccgaagaccc
      721 cgccaatagc atgcataccg gtgaggttct ctgatcgtgt ctctcttatc ggtcccagcc
      781 cgcccacact tgagcggtgt tgggaggcag gatggagaga ggtcccagtg gccgacaacc
      841 gacagtattc aacaagaaga ttcatataga cgccaaatag ggaaggacct cgaattcaag
      901 gatatccgcc attcgaaccg aatcgtgaac ggaccgagcg tccagaagct cttgaatcca
      961 ggaagataaa gcccgtaaat ttccgccgga tagactgccc tgcccagcgg cctgaagaag
     1021 ttccacataa tcggccaatt gtccgaacca tcgatccaat gccgagggaa tgacgccgct
     1081 cgtacgcaac ggtatgagaa atccctgagc gtcggctctc aatgtcgagg caaaccgggt
     1141 gattgccggt gcagcattcg tcataatctc gggaatgttc tgcgagcagg cttcgatcgg
     1201 cccgacttcg tcgagtcgtt tattcaataa caggggttgg agatcgcgat ccgtgatcgg
     1261 gcgcccccca aacttcaacg aagtgaccag gcgatgccgc gcataggcgc gatcgctgac
     1321 ctgtgtcagg acctcgctga ctggttgatt atcactgacg tcccactgat cgtgatcgag
     1381 cgtaatacgc atcggacgac tcctttctac aaacaccagt aaataaatat ctcttcagga
     1441 tgctcaaaaa gaccgctggt ctcacccgcc caaccccggc gcgcctagac gcgccgttcc
     1501 gcgagcaagg ccgcaggcta atcaccgctt tatacggggt ggctgggatg atcccaactg
     1561 cgcgcgtcca acgagggctt tcttaagccg cgggttgcgc gagcactggg atcgtcccag
     1621 gtaccccgcc tcatttttta gcatcctgct agacggcact cggcatcatc ggcaagacgc
     1681 accgtcgtgc ctcggtctgc acaggtcccg ctgacaagag cgttcgggtt tgcgcgatac
     1741 gctgctgcca gcgtcggtag gcctgtacgt gtcgatcggc caacatcgtc agatccacag
     1801 catcatcgtt atggatgtct ctgatcatgg ccacagtggc cgggccggtg gccgaatagg
     1861 ccatcattcg aggcaggagc tgttctcgct cttggcgaag cagcgcctgt tcccgttgta
     1921 gcgttgcgac cggcagcgga tgccgtttcg tcagaccgac gatatgcatg gcacgttgtt
     1981 ccaaccgccg caaagggtga accgcttctt tcatccaggc caacgcagag gcttggttcg
     2041 ggatggcttc gaggttcgga ggtaactgac tcggcttacc cgtgaaatct tggtaccaga
     2101 atctcctcca ataggcggca taccatgccg tatcggaatc gacgtgtcct gctcgcatga
     2161 caaaggagcc aagtccatcg gcatcggcgc cggactgata gacacgcaga cccgtgctcc
     2221 aggagggaaa cggtccgagc cccagtgtgt ggaggcagag cttggcgact tcttcaggaa
     2281 tcaatcgatc tttgcacgca tggtgggcgc agatctggtc aaacccacag ggcgaacagg
     2341 cgagatccgg ttgaatcacc caatggcctg gcccgtaggg tcccgtttca tggtgatcga
     2401 catggccgac tgacaaatca atgaccgggg ttccgacgcc gacggcgata tgcatcggtc
     2461 cagtgtcgtt ggtgaggagc aagcgacagc gcgagagcaa accgacaagt tgatgtagtg
     2521 tcgtccggcc actggcatcg agcatcggtg cagttcctcc gccggctcga taggtcgctt
     2581 tggcctgctc gatggattcc gattcagcgg ccgtgccgat taggacaaat cctaggccgg
     2641 ggtgacggct caacactgcg agcgtccggc caaaatactc cggtcgccag gctttcatga
     2701 catcgctggc gccaacctgc acggcgatcc actgccggac agtggtggtc tgagaacata
     2761 gaaattcgtc ggcccagtct ctcccatcgg ctgggacggt aaccgacagc ggcgagaaag
     2821 tgccgggacc gctcccgccc atggcataga ggtccaccag attgaaccgg ttgaatcgtc
     2881 gatgccggtg cagatccgtg aagtaagaca gccagggatt gtgtacgatg ggattgccgt
     2941 caggccctgc ggtaatccct cgtaggtccg gtgcgccgat gtaggatgcc aataaaccgg
     3001 tctggcgatt gaaagtgaga ttgacgatgc gattgtagcg cgcatcccgt agcggtgcag
     3061 cccccgtggt catctcctgc atcagcgtga caaggctctt ggattgcgtg cggcagtcat
     3121 caagtaatcg agtaaaatcg caactgatga cctgccggag tccggtaagg agagctgccg
     3181 tcggcgcgaa actgcggtcg acgaccaggt caatcgcgac atcgggccat tcctggcgca
     3241 accgggacaa caacggacca gtctgaatca gatctcccat acgagtcacg ttgatgatca
     3301 gtacttgctt cttcatatta agtcgaaatc tttcaggggt tcaggctgaa ggctgaaggt
     3361 tgtaaattat ctcgtttcaa acccttcagt ctaaaaacct tcggcctatt tacttgcgca
     3421 gctacaccaa atctttcgtc tcggaccgat actcgtccaa catgagcacc atcagatctt
     3481 ctctggatag ggtgcccatg ggcccgcgtg aacgaatctg ggccgcaagt tgcttcagct
     3541 ccacccgttg gccggcggga aatgttcgca gggttgcagc taacgacgga aatgagtctg
     3601 cagcggttga cgcaagtgct gctgcttgac ggcctccttg tagaattgaa ccgatgcgat
     3661 ccgggctcgc gacaccgatc tgggacagga ggtcgcgcat ccggtgagcg taggtatgtt
     3721 cggtgagtac gcgctgacgg gcttgccccg ccatggcgag gcgctgttcg ggctcgtgga
     3781 gccaacggga aatttcacca gggagttcgt cgggagacga gaccgtggca atctcacttg
     3841 aagtgaaaag gtccggcatc agcgatcgac ggtcggtaag ctgaaacgct ccacaggaag
     3901 ccagttcgaa cgtgcgcgga ttcacaaaat ccccgtcggg atcgagtccg gccccagccc
     3961 aagaatgcaa gttgagattc accgcactcg cattgaagac tttttggcag gtgtcggtat
     4021 cgatccgtgt tccgtgacgt tgaagaacgg gggccagatc ggtcgcgcca tcccactcat
     4081 tgccccagag cttaaatctc cattcgcgct tgaggagtcg gggaaagatc gcgcggcggt
     4141 tcgcatagcc tgcgccgaca aatgagacat cggacccgta ttcctcctgt tcagcctggg
     4201 tcaattccaa ggggcggtgt acggaaggat cggccgccat gggaaggtag cgcacatcct
     4261 tcgcacctgc ctgcttgagc gcggcaatgc agggctcctg ctggatcaca aaccaaaagt
     4321 catagccggc cgccagctgt tgccaatagg tcaaatgccg gtaattctcg acgaaccaca
     4381 tcgccgtcag aaaccgcttg cgtctgagat gctccaacat cgggagaatg agtggcgcct
     4441 gtgcaagcga caacacaaga tctggtggat cttctgccaa ctgagccatc gtgatccgac
     4501 tgagcatctc cgcgtactta ctttgcaagg ccaaccgatg ttgggagttt ttgagcaact
     4561 ccagctggtg gtagctcgtg gcatgaatgc tatgatcgag ccatcggacg tgatgtccca
     4621 gggattcgag cgcacgcacg acgaaccctg tagtagggag ggagccgcca tagatcggcc
     4681 cgacaaccgc aatacgcagt tgcccgccgg cctgcgccgc gacctgccgc tgcaaagccg
     4741 tttcaatcgc gcgatggcag tccgcatgta acgacgcagc cggcgtatag gtaacgagcc
     4801 gcagaggatg gccggcacgg gagatctgtt cactgatcac ccctgttgtg ccccaggccc
     4861 aggatacccg gtccatccag ttaccccagg tgcgcgcggc ggccgcatcg gccaagacgc
     4921 tcagatcggg aacgaccaga acgaccggta cagtccgagg caaacgggca cacaaggctt
     4981 cgatgtgata gaggagtccg acgccgacca cgacaatggt ttctccatcc tgatagctct
     5041 ggctttcggc ccagacttgc gcctctttga tgggatcgta tgcactgtga atccatcgcc
     5101 catgcgcacg cgccgacggc acacctgtgc gtgccggcat aatttccagc gtgcctcctc
     5161 cgctctcgcc gagtgccgct gccagagcag aatcacgctg cgccagtcgg ctgacattgg
     5221 cctcaaagga attcattaca catcctgcag caaccggtgc tgttcccata attgatgcca
     5281 gactgcggcc cgctgatcgt cttgtgcgta ttcttcgccc actggccgaa agatggctcc
     5341 ccattcgtca tggtgagatt gccataaagt ccacccggca acgggctctt gttgccatgt
     5401 cgaggcgggc tcacgaagca atgccacgct ggatcgaatc ggccaggtct tcggcatggc
     5461 gcgatcggct tgccacgccc aatggccctg cccatagggt ccggtttcat gcacgcgcgc
     5521 gcgtgaaaag tagaacccca gcgcctgcgt gccgacagcg gtcgccagat gcagtggccc
     5581 cgtatcggca ccgacggccc agtgacatcg cttcaggagc gtggcgagtt gccacaaggt
     5641 cgtgcgcccg gtggcatccc acacacgtcc tgcgatcatt ggagagagcc catcttgaat
     5701 ggccatggcc gccgcctgat cgcgtgcggc cccgaccagg acgacggcgc aaggtgattc
     5761 gctggcgagc actgttgaaa tccattctat ccagaccgtt atagggattg atcgatccgc
     5821 gtcgccagct cccaccacca cggcaatcca ttgcccgtcc aggataccga tctccgccaa
     5881 gtcaccagga agatcgatcg agggaagttg tatgacaggc ggaaccctcg gaggagtgac
     5941 tccgcagagt ccacagaacg tatcggagag atgaatgcga ttggagcccc gtgcctgtgc
     6001 gacatgacgc agatacgtcg cccagggtga caactcttca tccaatggcc cgcgcaggtg
     6061 ggggccctgt acctctgtcg ccaacagggt gccggccagg atagcccgtg ggtgatgatt
     6121 cagcacgtag gccatcgcat agggctcggt cgtcaaccct gcaaggtgcc gctcaacgtc
     6181 gcgcaaccat gagggctgaa actctccgat gaaagactca gcccacgcgt gccattgcgc
     6241 cccgttccac tccagaacct gcccgacatc cggaaagagg cgagcgagat ccgccagcgg
     6301 cgccggacac agcatgtcga gcgaccggtc cggataccga gccgagagtg acgtgatggc
     6361 cggcaagctc tggaccagat cgcccaatcg ggcgagttgg atcatgaggg catgtggggt
     6421 catggatcgt caagactgcg gaacaaattc aatcaatgta tcgtgtggcg acaacgcccg
     6481 tgccagctcc tcaagccccc cataggaagg cgccagggtg cagagtgtct ggtattcctg
     6541 ttgagcagcc tccgtctgtc cgcttcggat gagcacccct gccagtgcat atcgaacgga
     6601 caaatcggcc gggtttctca atacaaaacc ttggagtcgt tcgctcagtt ccgtccacaa
     6661 gccgcgcgcg ctgccagctc gtagaagcca atggatcgcc tcggcatcgt ccggatattc
     6721 gttcagcacg gtactgaaat cgttccatgc ccggtcgatt tcgccttggc ccactcgcgc
     6781 catccccaac cccatcatgg atttccgcct gtctccttga tgagtcaagg cagtcaggaa
     6841 cgcctgttcc gcttcggcgt agtgcgcctg ctgcatgtcg aggatgccgc gtaaaaccca
     6901 accctcacca tcctgcggaa actgcttcag cagtacgtcc agatgagagt ctgcctctga
     6961 caaattccct tcttctaacg ccattcgtgc cagaaccctt ctggcggtcg ggtcatcatg
     7021 atgctgcacc agtttctgat aaaattgtcg gctcaatgac gcatggcttg tcttggtgca
     7081 caaccaggcg ccccaaccga gaacatctgc gacggagggc cattcatccc accgggacaa
     7141 aagagactcc acctgagtga tgtcttgacg aaaggctgca cgttctgtgt cggccacgag
     7201 cttccatgaa gccttctcct gatcgccacg aagagggcga agttccagct gctctttgtc
     7261 ctggtccttc gtatggacag ccaggtaacc atcttcgtaa tagtagaggt cttcgtcagc
     7321 caaccaccat cgctgtcccc atcgactgag gaaccggagc gcattgtcat gatcatgagc
     7381 tttccgtcct ggcgtttgac tttcgaggtg atagaggaca cttaacggtt gatagacaat
     7441 ctgccaaccc ttgtcgccaa ccttcaagca cagatcgatg tcttcaaacc cgttttgaaa
     7501 ttgttcgtcg aaaccgccct ctgcctcgaa gatctctcgc ctgattaaca tgcaggccgc
     7561 cgtaacggat tggaatgttc gacgctggtt gaccgccgga tggtcccgat ggacccgtcg
     7621 atagagatgg tagggcatga gcgcttccct cgagaagacg actcctgcat gttgaatcgt
     7681 atcgtcttcg tagagcagtt tgcttcctac aattccgact tccggatggg acgtgacctc
     7741 agcaaccaac gcggtcagcc aatcactcag cggaatcgta tcgttgttca agaagatgag
     7801 gaagcgtccc cgcgccgccc gagcgccctg gttacaggcc ctggcgaaac cgagattttc
     7861 gctgtttcgg atgatctgaa tatcgccgct cagctgtgca aggaact
//
LOCUS       DATIPF010000007         3467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1340, whole genome shotgun sequence.
ACCESSION   DATIPF010000007 DATIPF010000000
VERSION     DATIPF010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VLL06_00185"
     CDS             complement(<1..970)
                     /locus_tag="VLL06_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HSS29413.1"
                     /translation="MHIIVAVAVLGNMAAIDHVVSAQEPEQPAVSPREEQLREQLKGI
                     LHELDELQKERERALPSGERPQAVIEKVSPQVSEAMPEYELSDLSIVSKRVYKRPEGV
                     SLSATIPAETDAQPTRTMKESMESLPGIVLRQANGPRDFSIMIRGQGAKTAFAVRDIK
                     IYEDGIQQTQSDGLSRLDMQDPWFMRSVEVTRGASSSLYDNYALGGMVQFRTRRGSDI
                     NGVETFLSGGSYGYQKYGLAVGQETERLDIAMFGSEVKEDGFIQNSNYNTQTLNLNFR
                     FKVDDKQNFYFKAISNWLNTKVPTRLTQSQFIANERQAGGAQTTCTP"
     gene            complement(1423..1977)
                     /locus_tag="VLL06_00190"
     CDS             complement(1423..1977)
                     /locus_tag="VLL06_00190"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSS29414.1"
                     /translation="MHRLLIRIGIAVLLSLMSIISAGPRFALASDPFVSLKMSQLPAG
                     SMAAPFALTTLDGKVVKSSELAGKVVLMNFWATWCGPCKEEMPSLARLQKQLDPAHFV
                     LMTVTTDVQPQGITHFLSQLGVSVPVLFDEDQEVSRSFMVRGLPTTVIIGRDGTLAGR
                     AVGPRAWDSPEAVAVMRQVMERGK"
     gene            complement(1979..3265)
                     /locus_tag="VLL06_00195"
     CDS             complement(1979..3265)
                     /locus_tag="VLL06_00195"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HSS29415.1"
                     /translation="MLERTFRIKRLGWVVGMVMSTLAFISTAMATPDSPVVFGPKLTT
                     DHPVRVVVGPSIRIDDEGRISLAWVEEDKDIRSVLYAGTEQAGGRIGTPVKVNQSAES
                     PYMRQESPALAVVGDNVFLTWALTHPKITQDKPFSNELRLSRSADGGKTFLPSILVND
                     DEQVIGHSFDSIHVAPDGVVHVAWIDGREGKKDPGTFVTRSTDHGRTVAKNFKVDEST
                     CVCCRTSLTTGPDGTLYVAWRKILPGDLRETVVARSTDGGQTFSSPVIVGHDKWVFPG
                     CPHRPASIGTDRFGRLYVVWYTEGVDDTPAVFLAYSDNRGDSFSPKQKLNVSKGTFPD
                     HPQMAVDPEGRLVIVWEEQSPVRREIVSSVSLDRGQSFSTPQKLNEKKAQTPSLSMNS
                     KGLAALAWMEHAMPVHRMVVQTLQLPPAKVVARQEP"
BASE COUNT          726 a          998 c          981 g          762 t
ORIGIN      
        1 cgggcgtaca agtcgtttgt gcgccacctg cctgccgctc gttggcgata aactgcgatt
       61 gggtcagtct ggtcggcact ttcgtattca gccagttgga aatcgccttg aagtaaaaat
      121 tctgtttgtc atctaccttg aaacggaaat tgaggttgag cgtctgcgtg ttgtaattac
      181 tgttctgaat aaaaccgtct tccttgacct cgctcccaaa catggcaata tcgagtcttt
      241 ccgtttcttg cccgacggca aggccgtact tttggtaccc gtaggacccc cccgacaaga
      301 aggtttccac gccgttgatg tcgctccccc gtctggtcct gaactgcacc attccaccga
      361 gcgcgtagtt gtcgtacaag gaagacgacg cgccacgagt cacttcgacg ctccgcataa
      421 accagggatc ttgcatgtcg aggcgtgaga gcccgtcgga ctgtgtttgt tggatgccgt
      481 cttcgtagat cttaatgtct cgaaccgcaa aggccgtctt agccccttgg ccacgaatca
      541 tgatgctgaa gtcgcggggc ccgttggcct gacggaggac gatgcctggc agggattcca
      601 tcgactcctt catcgttcta gtcggctgag catccgtttc ggctggaatg gtggcggaca
      661 acgacacccc ctccggccgc ttatagacgc gcttgctgac gatgctgaga tcggacaact
      721 catattccgg catggcctct gagacttgcg gtgacacctt ttctatgacc gcctggggcc
      781 gttcgccaga aggcaatgct ctctctctct ctttctggag ttcgtccagc tcgtggagaa
      841 tccccttaag ctgttcgcgc aattgttctt cgcgcggcga cactgccggc tgctccggct
      901 cttgcgcgga aacgacgtga tcgattgcgg ccatgtttcc gagcactgcg accgccacaa
      961 tgatgtgcat aactctcttg gatgacacca tggcccatac tcctccttgt gagaatgacg
     1021 ctgacacacc gctctccaag ccgggtttca ggccgcacag ttcatggcct gcccgcgcag
     1081 ggaaatgacg aatgaatggt gagtgggtgc gacgttatga acgaggggga gcacgggact
     1141 gtgccgactg ctgggagagc acggacgata gggaaacggc gaccagcagc accatcgtca
     1201 caaggtgaag cggctgtgtt tgtgacgtgc ctgcgggaag gtcaacggtc gggttggcgt
     1261 ggcaggccca ggcgcataga gatgaatgcg tgacgccgct tgtattatga tgatggactg
     1321 agcgaggctg gctctcgtgc gcaaaaagac aggccatggc agtggttgag agcgtcaggt
     1381 acagcactgc aacggccaga gctagcggga cgaagccttt cctcatttcc cacgctccat
     1441 gacctggcgc atcaccgcca cggcttcggg actatcccag gcacgaggtc cgaccgctcg
     1501 cccggcgagg gtgccatccc ttccgatgat gaccgtggtc ggaaggccgc gcaccataaa
     1561 ggaccgtgat acttcctgat cttcatcgaa gaggaccggc acgctgactc ccaactgaga
     1621 gagaaaatga gtaatgccct ggggctgcac gtccgttgtg acagtcataa ggacaaagtg
     1681 tgcaggatcc aattgtttct gcaatcgggc cagtgagggc atttcttctt tgcagggtcc
     1741 gcaccaggtc gcccaaaaat tcatcagcac gactttcccg gccagctcgc tcgacttcac
     1801 gaccttgccg tccaatgtcg tgagtgcaaa gggtgctgcc atgcttccgg caggcaactg
     1861 gctcatcttg agcgagacaa agggatcgct ggcaagcgca aaccttggac cagcagagat
     1921 gatgctcatg agagagagca atacagcgat cccaattcgg atcagcaaac gatgcatact
     1981 agggctcctg cctcgcgacg actttggcag gcgggagctg aagggtttgt acgaccatgc
     2041 gatgaacggg catcgcgtgc tccatccaag ccagagctgc gagccctttc gagttcatcg
     2101 agagggatgg tgtttgggct ttcttttcat tcagtttctg cggtgtactg aacgactgcc
     2161 cacgatcgag cgaaacactg gagacgattt cacgccgaac aggcgattgt tcctcccaga
     2221 cgatgacgag acgtccttca ggatcgaccg ccatttgcgg atgatcaggg aaggtcccct
     2281 tggagacgtt cagtttctgt ttcggtgaga agctgtctcc acgattgtcc gagtaggcta
     2341 ggaaaacggc cggagtatca tcgacaccct ccgtatacca gacaacatag agccggccga
     2401 accgatccgt cccgatcgag gcgggccgat gtgggcagcc ggggaacacc cacttgtcat
     2461 gtccgacgat cactggactg ctgaaggtct gcccgccgtc ggttgaacgc gcgacgacgg
     2521 tctcgcggag gtcacccggc agaatctttc gccaggccac atagagtgtt ccgtccggtc
     2581 cggtcgtcag agatgttcgg caacagacac aggtgctctc atccactttg aagtttttcg
     2641 cgactgtgcg gccatggtcg gttgagcgtg tcacgaaggt gccgggatcc ttcttgccct
     2701 cccgtccatc gatccaggca acatgcacga ccccgtcagg ggcgacatgg atcgagtcga
     2761 agctgtggcc gatgacctgc tcatcgtcat tcaccagaat ggacggcaga aatgttttgc
     2821 ccccatcagc ggaccggctc aggcgcagtt cgttcgagaa cggtttgtct tgcgttatct
     2881 tcggatgggt caaggcccat gtcaggaaca cgttatctcc aaccacagcc agcgccggag
     2941 attcctgccg catgtagggt gactcggccg attggttcac cttgaccgga gtcccgatcc
     3001 gtcctcccgc ctgctcagtt ccagcataga gaaccgaccg tatgtccttg tcttcctcca
     3061 cccaagcaag ggagatacgg ccttcatcgt caatccggat cgaaggaccg accaccaccc
     3121 ttaccggatg atcggttgtc agtttcggac cgaacacgac cggcgaatct ggcgttgcca
     3181 tagctgtgga tatgaaggcc agggtactca taaccattcc gaccacccat cccagtctct
     3241 tgattctgaa tgttcgttcg agcatggggc acctcatttt gatcgacgaa tggataaggg
     3301 aacggcacga ttacttgaac cctggtgcac actcactccc ggcatcgacc caagcgattc
     3361 cttctgcatt ccgatatcgg gggctagcgc atcgcctcca atcgcctaac tcgcaaggct
     3421 atagacaatt ggcacataga tggccagttc ggatgcgctg atcggct
//
LOCUS       DATIPF010000008         3030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1327800, whole genome shotgun sequence.
ACCESSION   DATIPF010000008 DATIPF010000000
VERSION     DATIPF010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327800"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1358
                     /locus_tag="VLL06_00200"
     CDS             162..1358
                     /locus_tag="VLL06_00200"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HSS29416.1"
                     /translation="MPTSFEWILIVFSSLIVLSILTIKLSNRFGIPSLVLFVAIGMLA
                     GSDGPGGMYFDDPSLVKSMGILALIMILFSGGLDTEWTGVKPILWKGLSLSTIGVLIT
                     ALLVGVFVAWVQGFSFLEGLLLGAIVSSTDAAAIFMVLKSRSSKLPKRLAQLLELESG
                     SNDPMAVVLTIALIRLLTEPSTSFGELVAFFVMEMAIGTVMGIAMGEVMRRMFNALDL
                     GLEGIYLVLSLALVLLTYGVTVSLHGSGFLAVYLAGLIMQRKPFVHQENVLRFHDGLA
                     WLMQVTMFLMLGLQVFPARLVPVAWVGLLVSLFLIIIARPAAIHTALAFSPMPYREQI
                     LVAWAGLRGAVPIILATFPLLAGVKQADTIFHLVFFITLTSVVMQGTTISLATRMLKI
                     DDPPSK"
     gene            complement(1454..2398)
                     /locus_tag="VLL06_00205"
     CDS             complement(1454..2398)
                     /locus_tag="VLL06_00205"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HSS29417.1"
                     /translation="MAIKAQFPDEQSTEGAFTRQPDAFTDWVTANGSSGYPAASGRYH
                     LYVSWACPWAHRTIIVRKLKKLEDVIGMTVVDPIRDERGWAFREGLSHSLDPVNGFQF
                     LSQAYRATDPNYRGRFTVPVLWDRVTKRIVTNSDDDLMRMFNGEFNRFTESPIDLYQD
                     GIRKEIDDLNTFIYENVNDGVYRAGFATSQQAHERAVWRLFEALDQLEARLARQRYLF
                     GSQFVETDWRLFVTLVRFDAVYHGHFKCNLRRISDYPNLFGYLKDLYQTDGIADTVNF
                     DHIKRHYYMTHDDINPTRIVPLGPDQDLTAPHHRAHLP"
     gene            2748..>3030
                     /locus_tag="VLL06_00210"
     CDS             2748..>3030
                     /locus_tag="VLL06_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29418.1"
                     /translation="MHRVGRIGFMGLGIALAGCLTGAPVFAGDKPAKAPIEDGSKVMI
                     TSPKDGDKVSDSFELKYELTKGSQATHVHAYVDEQYQKGFAGEIKGLSKG"
BASE COUNT          585 a          766 c          928 g          751 t
ORIGIN      
        1 ggccttctcc gccccgttcg aggatcaatc caagaccagt gtcagacatt gtttgccaga
       61 gatgagagcg cgttggctca tattgtggct agcacggatt ggtcgtctgt gctatagatc
      121 gcatcggttc gcgtccgaca aactcgaaac acactacatt aatgccgacg tcctttgagt
      181 ggatcctcat cgttttctcc agccttatcg ttctgagcat tctgacgatc aaactctcca
      241 atcggtttgg catcccctcc ctggtcttgt tcgtcgccat cggcatgttg gcgggatccg
      301 acggtcccgg cggcatgtat ttcgacgatc cgtctctggt gaagtcgatg ggaatcttgg
      361 cgctcatcat gattctgttt tcgggcggat tggacacgga gtggacgggg gtaaaaccca
      421 tcttatggaa ggggctgtct ctctcgacga tcggggtgct catcacggca ttgctggtcg
      481 gagtgtttgt cgcttgggtt caggggttct cattcctgga gggcctctta cttggcgcga
      541 ttgtgtcgtc caccgatgct gcggctattt tcatggtgct caaatcgagg agttccaagc
      601 tcccgaagcg cttggcccaa ttgctcgaat tggaatccgg cagcaacgat ccgatggcgg
      661 tcgtgctgac gatcgcgctg attcggctgt tgacggaacc ctcgacctcc tttggagaac
      721 tggtcgcatt cttcgtgatg gaaatggcca tcgggacggt gatgggcatt gcgatgggcg
      781 aggtcatgag acgaatgttc aacgcgctcg atttggggtt ggaaggcatt tatctggtgt
      841 tgtcgttggc gctggtgctc ctgacctacg gtgtgaccgt gtccttgcac ggaagcggct
      901 ttcttgccgt gtatctggcg gggctcatca tgcagcggaa gccctttgtc catcaagaga
      961 acgtcttgcg ttttcacgat gggctggcct ggctgatgca ggtcaccatg tttctgatgc
     1021 tggggctgca agtattcccg gcaaggctcg tgccggtcgc gtgggtgggc ctgctagttt
     1081 cccttttcct gattatcatt gcacgccctg cggcgatcca cacggcgctg gcgttttcgc
     1141 cgatgccgta tcgagaacag atccttgtgg cctgggccgg gctccgtggg gccgtgccga
     1201 ttatcctggc cacctttccc ctattggccg gagtcaagca ggccgacaca atcttccacc
     1261 tggtgttttt tattacgctc acgtccgtcg tcatgcaggg cacgaccatt tcattggcga
     1321 cacgaatgtt gaagattgac gatccaccat cgaagtaagc ctcctcctgc caacgcctct
     1381 tcgtgtccgg gctctcctgc tcacttgatc tagccggttc gtctcacaca gaccagagca
     1441 accggtctgc ccgctatggc aagtgtgcgc ggtggtgagg agcggtgaga tcttgatcgg
     1501 gaccaagggg gacgatacgg gtcggattga tgtcgtcgtg cgtcatatag taatgccgct
     1561 tgatgtggtc gaagttcacg gtgtcggcga tgccgtcggt ctgatagagg tctttcaggt
     1621 agccgaagag gtttgggtaa tcgctgatgc gcctgaggtt gcacttgaaa tggccatggt
     1681 agacggcgtc gaagcgcacg agggtgacga acaagcgcca atcggtttcg acgaattgtg
     1741 aaccgaagag atagcgttgg cgcgccagcc gggcctccag ctgatcgagg gcctcgaaga
     1801 ggcgccacac ggctcgttcg tgggcttgct gcgatgtggc gaatcctgcg cgatagaccc
     1861 cgtcgttgac gttctcgtag atgaacgtat tgaggtcgtc gatttccttc ctgattccgt
     1921 cttgatagag gtcgatgggg ctctcagtga aacggttgaa ctcgccgttg aacatgcgca
     1981 tgaggtcgtc gtcggaattg gtgacgatgc gttttgtcac tcggtcccac aacacaggga
     2041 cggtgaaccg tccccgatag ttcggatcgg tggccctgta ggcctgactg aggaactgga
     2101 atccgttaac gggatcgagc gaatggctga gtccttcgcg gaacgcccag ccccgctcgt
     2161 cgcggatcgg gtccacgacc gtcatgccga tgacgtcttc gagtttcttg agttttcgta
     2221 cgatgattgt ccgatgtgcc caggggcagg cccaggagac atagagatgg tagcggccgc
     2281 ttgccgccgg gtagccggaa ctcccattcg cggttaccca gtccgtaaag gcatccggct
     2341 gcctcgtgaa ggcgccttcg gtgctctgct catctggaaa ttgtgctttg atggccatgg
     2401 tccggctctc actttcttct accgtaccac atttaattgc atgggggcat ggaatggcca
     2461 ggtgtctttt tcactcacag atctctcccg atcgaatggt ggttccgcga ttcgcttagt
     2521 aaagactaga ctgagccatt tctctcaaag aaggtacaga agtagatgcg cttcgtctta
     2581 tgacggtccg gcgatctgcg aatgtgcgga ttgactgaga taatcgtgaa aagtaagata
     2641 tgccggtgtt cttaaccgaa gattggttga gaacgggacg agaggaaatc ccgaaaggga
     2701 tggtacctgg cacggttcat tcagtcgttt caaacaagga ggtcgtgatg catagggtcg
     2761 gacgtatcgg attcatggga ttagggattg cgttagcggg ctgcctcacg ggagcaccgg
     2821 ttttcgcagg ggataaaccg gcgaaagcac caatagagga tgggtcgaag gtgatgatta
     2881 cctctcccaa ggacggggac aaggtcagcg acagttttga gctgaagtac gagttgacca
     2941 agggatctca agcgacacat gtccatgcct atgtggacga gcagtatcag aagggcttcg
     3001 ccggggagat taagggtttg agcaagggaa
//
LOCUS       DATIPF010000009         6644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1504727, whole genome shotgun sequence.
ACCESSION   DATIPF010000009 DATIPF010000000
VERSION     DATIPF010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504727"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /gene="hemW"
                     /locus_tag="VLL06_00215"
     CDS             <1..1125
                     /gene="hemW"
                     /locus_tag="VLL06_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HSS29419.1"
                     /translation="PLGLYLHIPFCRQRCDFCAFYLEIHREDPAETFVRSLIHEIQLS
                     THQHVAAGRPIQSVYLGGGTPTVLAATQLLEILSEIRAALTLASDCEITVEAHPSTIS
                     EQDLAQLHQAGTTRISFGAESMDDGDLTRIGRPGAVCETVTAVTHARAAGFTNINLDV
                     MYGLPGQSLGSWQRTLIHCLALEPTHLSCYALTVEEGTRLASNIQRGRSPALDEDLQI
                     EMDQVAQQILSNAGYERYEVSNYAKPGYACRHNLLYWTNGEYLGFGPSAQSYLAGTRF
                     GNVADVAVYNTSLAENRLPIEDRSKLSEKEQLRDAVIFGLRLIRGVPTFHLHQHAENY
                     GHTAVTAQLFAEQLIEEDGERSRLSARGRLQADTIAGQLY"
     gene            1305..1427
                     /locus_tag="VLL06_00220"
     CDS             1305..1427
                     /locus_tag="VLL06_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29420.1"
                     /translation="MPTQATQFGEETEEDRVKALADAEQKNLWEATEVIDMDKF"
     gene            complement(1726..2799)
                     /gene="cax"
                     /locus_tag="VLL06_00225"
     CDS             complement(1726..2799)
                     /gene="cax"
                     /locus_tag="VLL06_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HSS29421.1"
                     /translation="MRYLFTSWLDFLLIFVPATLVLELLRADPLLVFVSAGLAIIPLA
                     GLLGRATEHLTTHVGAGIGALLNASLGNAAELIIALVALREGLHDVVKASLTGSIVGN
                     ILLVLGVSMFAGGIKYERQKFNQTAAGMGASLLLLAAVSLIIPALFHFTAADRGIVIE
                     QRLSFIISCVLFMTYIASLLFTLKTHRHLFAGDAHNASDLGEQPWSKRACIIVLAVVT
                     CLVALMSEMLVGALEPASQQLGLTQVFVGVILVALVGNAAEHSTAVMVALKNKMDLAY
                     GIAVGSSLQIALLVAPLLVFASYLFGAPLDLVFTPFEVAAVTISVLIVGFVAIDGESN
                     WMEGVMLVGVYVILAIAFFFLPA"
     gene            2834..3514
                     /locus_tag="VLL06_00230"
     CDS             2834..3514
                     /locus_tag="VLL06_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29422.1"
                     /translation="MNQPRAPHALFYPFHLCHPDTMVRLLTRFATVHFRDFMALQLTP
                     MSGMTAFQDRMGMSFPDLIDSGRLVQGYDVSGPLPLTVAATIDRDVHDPVWRARFHTA
                     LCRDRRFQRGLFEPSHSLRIGNSLVPGPAALLHLMNDSFRRRVYDLGLVRGLSKSSLT
                     LEEGYQFEYGLALVKTSASHVYTQTLALAHQLQPVTDSLSHFALYAQSCIREHWPRSN
                     HLLIRTGY"
     gene            3548..3844
                     /locus_tag="VLL06_00235"
     CDS             3548..3844
                     /locus_tag="VLL06_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HSS29423.1"
                     /translation="MAGINTPLAPPAPTETTSLDTSADLNARVIVFNCECHTYEQVIA
                     LFCQYIPGMNSTRAFELAWKIDHDGEAIVFAGGLEQAEEIAAKLAGGGLRVAVQ"
     gene            complement(3856..4248)
                     /locus_tag="VLL06_00240"
     CDS             complement(3856..4248)
                     /locus_tag="VLL06_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29424.1"
                     /translation="MERRAASHTEEEEMNHRRRLLNAAKKGDQKAIGKLLELYQVRIY
                     SGDMVTKMNKTSATHKLQAQLAEGKPVKGGSGSHGKKTASTKPVPEKRAVAKVVGRIA
                     ISKKQTKPKAKPKPKARPTKAAKKKHKK"
     gene            4572..5252
                     /locus_tag="VLL06_00245"
     CDS             4572..5252
                     /locus_tag="VLL06_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3386 family protein"
                     /protein_id="HSS29425.1"
                     /translation="MEQHQRKQEEHTVADDPKAREMLRQAFEKTARWQKDFKGFTADL
                     TVNVNGKETGGPVMVKGPREVSVQLGDGEVQKWAQEQLGMIAVHRGARTFEDSDGKYS
                     LTMEEDGHPFGTKLIIHGSHSFYRLKDNRITQINRTMAHPGMTPLAFTINVEESATTQ
                     DQKNLTTKYSVFYSSPSDGKLTNVECFTDTHVRIGSSDLPATRRIISYEQGEVIVKNL
                     TFTNHKLI"
     gene            5323..5754
                     /locus_tag="VLL06_00250"
     CDS             5323..5754
                     /locus_tag="VLL06_00250"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HSS29426.1"
                     /translation="MDLRTSFPHSMRYQMNGYVHLARMIDKCRAVLAGTEGEYIYPCP
                     MDDRLMEFAGITADQFTASVKASPSDEAVADWFTRTAQAHAPAELDAWNRMMLTRGPS
                     SPKKRAYFNKLRNQIDPSRTDLTAWADLQDLEEGRTVPRRS"
     gene            5751..6551
                     /locus_tag="VLL06_00255"
     CDS             5751..6551
                     /locus_tag="VLL06_00255"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HSS29427.1"
                     /translation="MTYAKALRVFRRAVTTALLVMLVTVQFVLPPSGWAVERAEAEET
                     ARLLAKLLKAGRAVIEQNQQLIDDPQKGDKGLTPEVFEQQLIQEFRQQTGVDLSKLHS
                     TPASFSMPPQAKEMLPAFVLASKEVIREAQAVINQRGIGYKNFIPATYGSRAAALFSK
                     QSHVRLKQTTLHPRNPKNEPDDYEASVLKWLAGRPRAESYVSELADEGRTLRVMMPLY
                     YQKACLACHGEPKGDLDISGYPKEGYREGDLAGAITVVTPLAGGGVNR"
BASE COUNT         1597 a         1892 c         1790 g         1365 t
ORIGIN      
        1 ccccttggtc tgtatctcca catccccttc tgtcgccagc gatgcgactt ctgcgccttc
       61 tatcttgaaa ttcatcgcga ggaccctgca gagacttttg tccgatcact tattcatgag
      121 atccagctgt cgactcacca acacgtggcc gcaggccgtc ccatccagtc cgtctatctt
      181 ggaggcggca ccccgacggt actcgccgcg actcaacttc tcgagatcct gtcagaaatc
      241 cgcgcagctc tcacccttgc ttcagactgt gaaatcaccg ttgaggcgca cccctccacg
      301 atctcagagc aggaccttgc gcaactgcat caggccggca caacccggat aagcttcggc
      361 gcagaatcga tggatgatgg cgacctgact cgaataggac ggcccggtgc cgtgtgcgag
      421 acagtcacgg cggtgacgca tgccagagcc gccggattca ccaacatcaa tctcgatgtg
      481 atgtacggac tccctgggca aagcctgggt agttggcaac ggaccctcat ccattgtctt
      541 gcattggaac cgacacatct gtcttgctat gccctcacgg tcgaagaggg tacgaggctt
      601 gcgtccaaca tccagcgcgg acgaagtccg gctttggacg aagatcttca gatagaaatg
      661 gaccaggttg cacaacagat actcagcaac gccggctatg agcggtatga agtgtcgaac
      721 tatgccaaac cgggatatgc ctgccgacac aatctgctct actggaccaa cggcgaatac
      781 cttggattcg gcccgagcgc acagtcctat cttgccggaa cgagattcgg caatgtcgcc
      841 gacgttgcgg tctacaacac atcccttgcg gaaaaccgct tgcccattga agaccgcagt
      901 aaactgtcgg aaaaggagca actccgcgat gccgtgatat tcggcttgcg acttattcga
      961 ggagttccca catttcatct tcatcagcat gccgaaaact atgggcatac ggcagtcacc
     1021 gcacagttat tcgctgaaca gttgatcgag gaagacggtg aacgcagcag actttcggca
     1081 cggggtcgcc tgcaggcgga cacgatcgcc gggcagttat actagtaggc tgttgacaac
     1141 gttcacgcca ccgcgcgtat tgactttgtc tttgggtggg atagaaactc aacggaccac
     1201 acagtggagt tgcaatgccc gttaatatgg atcctgccaa gaagtcgaaa agacagccgt
     1261 cgccactaga ggcagattcc tctccttcca agaaggaatc gacagtgccg acacaagcga
     1321 ctcagttcgg tgaagagacg gaagaggatc gggtgaaggc actggcggat gcagagcaga
     1381 agaatctgtg ggaggccacg gaagtcatcg acatggataa attctgacgc gaatgccccg
     1441 cgagactgac gggaaaagcc ggatactcgg aatcaagttt tgtgcgccgc acccgccatc
     1501 tttctactag gctacggaaa actttgtgcg tacgcaggat gctcaaaaag cccgttcagc
     1561 aaggccgcag gggagtcgaa accggaggcg taccctctga ggcgcacggt gcgacgaata
     1621 aggagcacca cgtctgtgcg cgccgccgag tggtgaggcg gccgggtccc cgttgaggat
     1681 ttcgatgacc cgagaacgaa gctggcggac tctttcagca tcctgctagg cgggcaggaa
     1741 gaaaaaggcg atggccaata tcacgtagac cccgactaac atcactcctt ccatccagtt
     1801 cgattctccg tctatcgcca cgaatcccac gatcagaacc gagatggtca ccgcagcgac
     1861 ctcaaacggt gtgaagacca gatccaacgg ggccccgaag agataactgg caaagaccag
     1921 caatggtgca acaagcaacg caatctgcaa actcgacccg acggcgatac cataggcgag
     1981 gtccatcttg tttttcagtg ccaccatcac cgccgtagaa tgttcagccg cattgccgac
     2041 aagggctacg agaatgactc cgacgaagac ttgtgtgagc ccaagttgct gcgacgcagg
     2101 ctcaagcgca ccgactaaca tctcgctcat tagggccacg agacaggtca ccacggcgag
     2161 cactataatg caggcacgtt tgctccaagg ttgttctcct agatccgagg cattatgagc
     2221 atctccggca aacaggtggc gatgagtctt caaggtaaac aataagcttg caatgtaagt
     2281 catgaatagt acacatgata taatgaagct taatctttgc tcgatcacga tgccacggtc
     2341 tgcagcggtg aaatggaaga gggcggggat gatgagcgac actgcggcca gaagcaagag
     2401 actggcgccc attccagcag cggtctggtt aaacttctgc cgctcgtatt ttattccgcc
     2461 tgcaaacatg gagacgccaa gtacaagtaa gatgttgccc acgatcgaac cggtgagtga
     2521 agccttgacg acatcgtgca gaccttctcg cagagccacc agcgcaataa tcaattcagc
     2581 agcattgcca agcgaggcat tgagcaacgc accgattccg gcaccgacat gggtcgtcaa
     2641 atgttcggtg gctcgtccca acaggcccgc cagtggaata atggcaagtc cagctgagac
     2701 aaagaccagc agtggatcgg ctcgaaggag ttcgagcacg agagttgccg ggacaaagat
     2761 gagtaggaaa tcaagccacg acgtgaagag atatcgcacg cgcagaccct agcatgacga
     2821 tgaagctttg tcgatgaatc aaccccgagc accccatgcg ctgttctacc cctttcatct
     2881 ctgccaccca gatactatgg tacggctcct gactcgtttt gccacagtcc attttcgcga
     2941 cttcatggca ctgcagttga cccccatgtc cgggatgacg gcgttccaag accgtatggg
     3001 catgagcttc ccagacctca tcgattccgg acgcctcgtg caaggctacg acgtgagtgg
     3061 accgcttcca ctgaccgtgg cagcgaccat cgatcgggac gttcacgatc ctgtttggcg
     3121 cgcgcgtttc cataccgcgc tctgccgaga ccgtcggttc caacgtgggc tattcgagcc
     3181 gtcccacagc cttcgcatcg ggaacagtct cgtgccaggc cctgccgcac ttctgcacct
     3241 catgaacgac tcgttcagac ggcgtgtgta tgatctcggt ctggtgagag ggctatcgaa
     3301 aagtagcctg accctagaag agggatacca gtttgaatat ggactcgccc ttgtgaaaac
     3361 atctgcctca cacgtctaca cccagacgct ggcgctggca caccaactcc aaccggtgac
     3421 agactctctc tcgcattttg cactctatgc acaatcctgt atccgagagc attggccgag
     3481 atccaaccac ttgctcatca ggacaggcta ttaaaagttg gaaccaagtc tgctagaatt
     3541 tgttcccatg gccggcataa acaccccctt ggctccgccc gccccgacag agaccaccag
     3601 cctcgacact agtgctgatc tcaatgcgcg tgtgatcgtg ttcaattgcg agtgtcatac
     3661 gtatgagcaa gtgattgcgc tcttttgcca atacattccg gggatgaact ccacgagagc
     3721 ctttgagctg gcatggaaga tcgaccatga cggggaagcc atcgtgttcg cggggggtct
     3781 agaacaagcg gaagagatcg cggcaaaact ggcgggagga ggtctacgag tcgccgtgca
     3841 atagtatagc ccgggctact tcttgtgttt cttcttggcg gctttggtgg gtcgagcttt
     3901 cggtttcggt tttgcctttg gcttcgtctg tttcttcgag atggcgatac gtcccacaac
     3961 cttcgcaacc gcgcgtttct ctggaacggg cttcgtggat gcagtcttct tgccgtggct
     4021 ccccgaccca cccttcacag gcttcccttc ggcgagctga gcctgaagtt tgtgcgtcgc
     4081 ggaggtttta ttcatttttg tcaccatatc gccgctataa atccgcactt gatagagctc
     4141 taaaagcttg ccgatcgcct tctgatcgcc tttctttgcg gcattcagca accggcggcg
     4201 atgattcatc tcttcttctt cggtatggga tgcagcccgt cgttccatgt ccatcctttc
     4261 attctacgcg ggcataaaac ccgcgcggcg cagtatactc gcaatgttca atattttcta
     4321 cagcctatat gagccaccag agcagccgct cgagacaggt gtgagggcac gacagccgaa
     4381 aattcaaatc tgctggaatt tggaacctca cttttatatt tcagctcgtg catgatgtga
     4441 tggtctcgcc catctcgggc gtagcacgct ttctccatgg ggcctgtcca gggcgcctga
     4501 gagcgcttgt ggcatatttc ggcgcaagcg tataatgctt ttttcatttg attcacgaag
     4561 gagatgacct catggaacaa catcaacgga aacaggaaga acacacggtc gccgacgatc
     4621 cgaaagcccg agaaatgctt cggcaagcct ttgagaagac cgcacgctgg cagaaagatt
     4681 ttaaggggtt caccgccgat ctgaccgtga acgtgaacgg aaaggaaacc ggcggacccg
     4741 tcatggtgaa aggaccccgt gaagtgtcgg tccagctggg cgatggcgag gtacagaagt
     4801 gggcgcaaga acagctcggt atgattgccg tgcaccgtgg cgcgagaacc ttcgaggact
     4861 cggacggcaa gtattcactg acgatggaag aagatggaca cccgttcggc acgaaactga
     4921 tcatccacgg atcacactcg ttttaccgcc tcaaagacaa tcgcattacc cagattaatc
     4981 gcactatggc gcaccccggc atgaccccct tggcctttac gattaacgtg gaagagagcg
     5041 cgacgactca agaccagaag aatctgacga cgaaatacag tgtgttttac tcctcaccca
     5101 gcgacggaaa gctgaccaac gtggaatgct tcacagacac ccatgtgcgc atcggatcat
     5161 cggatctgcc ggctacccgc cgcatcatct catacgaaca gggcgaagtg atcgtgaaga
     5221 acctgacctt cacgaatcac aagctgatct agcaatttct actgctgtcg cctctccctc
     5281 aaagagggag aggcgacggg aaacccgcat gcacggagct cgatggacct tcggacatca
     5341 ttcccccata gcatgagata ccagatgaac ggttacgtcc atctggcgcg gatgatcgat
     5401 aaatgccgcg ccgtgctggc aggcaccgag ggcgagtaca tctatccctg ccccatggat
     5461 gatcgactca tggagttcgc aggaatcacc gccgatcagt tcaccgcatc cgtgaaggcg
     5521 agtccatcag acgaagccgt ggctgactgg ttcacacgga cggcacaagc ccatgctcca
     5581 gccgagttgg acgcatggaa ccgcatgatg ctgactcgcg gaccgagcag tcccaagaaa
     5641 cgagcgtact tcaacaaact ccggaatcag attgatccct cacggaccga cctcacggcc
     5701 tgggccgacc tgcaagacct ggaggaaggc cggacagtac cgagacgctc gtgacctacg
     5761 ctaaagcact ccgagtcttt cgcagggcag tgacgaccgc cctgctcgtc atgctcgtca
     5821 ctgttcagtt cgtcctaccg ccatccggtt gggctgtcga gcgcgcagag gcggaagaga
     5881 ccgctcgact gttggctaag ttgctcaagg ctggccgggc ggtcattgag cagaatcaac
     5941 agttgattga cgatccgcag aagggggaca aaggattgac gcccgaggtg tttgaacagc
     6001 aactcattca ggaatttcgt caacagaccg gcgttgatct ctcgaagctc cacagtaccc
     6061 ctgcgtcttt ctctatgcca ccccaggcca aggaaatgtt gcctgccttc gtgctcgcca
     6121 gcaaagaagt gatccgtgag gcccaagcgg tgatcaatca acgggggatc gggtataaga
     6181 actttatccc ggccacctat ggaagccggg ctgccgcact tttttccaaa caatcgcatg
     6241 tcagattgaa gcaaaccacc ctgcacccgc gcaatcctaa gaatgaaccg gacgactatg
     6301 aagcctcagt gctgaagtgg ctagcaggcc gcccgcgagc cgaatcctat gtgagtgagt
     6361 tggccgatga aggacgtacc ctgcgggtga tgatgccgct ttactatcag aaggcctgtc
     6421 tggcctgcca cggcgaaccc aaaggcgatc tggatatctc cggctatccc aaagaaggat
     6481 atcgcgaagg agatctcgcc ggagccatca ctgtggtgac accgctggcc ggtggcggcg
     6541 tgaaccgata aggatcaatc gtgaatcgtc ctcgaatgat acaaagcggc aatgaaacag
     6601 ggaacaattt gaggtgtatg gcgtccctct cacaccggat tcgg
//
LOCUS       DATIPF010000010         2959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_797469, whole genome shotgun sequence.
ACCESSION   DATIPF010000010 DATIPF010000000
VERSION     DATIPF010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797469"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VLL06_00260"
     CDS             complement(<1..1175)
                     /locus_tag="VLL06_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSS29428.1"
                     /translation="MNDLRTRLYWIMGLRVALVTLMLGLSLAFQVVKGERVETFYTLI
                     IVTYILTIPSALLLRVLSASKTLTIFFWTQVAIDFLLETVLVSRTGGIESPFAVLYVI
                     TVAVASLVPRRRVGLLTACFCIIVFGLLTNIQLYGLIEGWGWLPPSRLTVPETFQTFG
                     VYALALLVVGILGGTLAEQLQQADHSLREKEQGLTRLQVFHENIVHSISSGVFTADAM
                     GCITSFNPAAQEATGYTITQVAGRPWREVFNWHPSQQSDEQLNGVVSTTTRFEVECKR
                     ADGNRLILGMTLSPLHEQGRQTGLVGVFKDLTQIRDLEEEMRRKDWLASLGEMSAGMA
                     HEIRNPLGALAGAMQMLRKDAIEDETNRRLMDIAIREATRLDNIITEFLQYARPPAL"
     gene            complement(1380..2597)
                     /locus_tag="VLL06_00265"
     CDS             complement(1380..2597)
                     /locus_tag="VLL06_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSS29429.1"
                     /translation="MATFAYVGRTRSGAVKKGELSAKTRDEAVDQLRKQSVVVTSLEE
                     KKGGGGGFKLSFGSGLTDKDLVVFTRQFGTMINAGLPLVQCLEILSTQSENKVLRETI
                     GEVKIQVEAGSTFSDALRRFPKIFDDLYVNLVHAGEVGGLLDTILTRLAQYIEKAMKL
                     KGQIKAAMVYPATIMGVAVAVITILMLFVIPIFAKMFLELSGGRVALPGPTQLVIDIS
                     NFFIGYWYVILGAIVGIIVGIKKYYGTVRGRLVIDKLLLRIPVIGDLIRKASVAKFTR
                     TLGTLLSSGVPLLDGMTICAKTSGNKVVEGTLLDARLSISGGKTIADPLAASGVFPKM
                     VTHMIAVGESTGALDAMLGKIADFYEDEVDQAVASLTALLEPAMMVFLGIVIGFIVIA
                     MYLPIFKMASAIG"
     gene            complement(2756..>2959)
                     /locus_tag="VLL06_00270"
     CDS             complement(2756..>2959)
                     /locus_tag="VLL06_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29430.1"
                     /translation="IDLTPTFLGKGTTFLQLSYVVMVIFLSSRQIDLTLVLPLLFGMV
                     AFTLLSGLHYLYRGIRHTSTIGG"
BASE COUNT          746 a          837 c          742 g          634 t
ORIGIN      
        1 ttgagcgccg gcggacgcgc atattggaga aattcggtga tgatgttgtc gagcctggtg
       61 gcctcccgaa tcgcaatgtc catcaaccgg cgattggtct cgtcttctat ggcatctttc
      121 cggagcattt gcatcgcacc agccaatgcg ccaagcggat tccgaatttc atgagccatc
      181 ccggcagaca tttcacccaa gcttgccagc caatccttgc gacgcatctc ctcctcaaga
      241 tcccgaatct gtgtcagatc tttgaataca ccgaccaatc cggtctgcct cccctgttcg
      301 tgtaatgggg aaagcgtcat accgaggatc aggcggttac catcagcacg tttacattct
      361 acttcgaacc gcgtcgtcgt cgacacgacg ccattgagtt gttcgtccga ctgttggctc
      421 ggatgccagt taaacacctc gcgccaggga cgcccggcca cctgagtgat cgtatacccg
      481 gtcgcttcct gtgccgccgg attgaacgac gtgatgcacc ccatggcatc ggccgtaaat
      541 accccgctgc tgatgctgtg gacaatattc tcatggaaca cttggaggcg ggtcagtccc
      601 tgttccttct cgcgcaacga atgatctgcc tgctgaagtt gttctgctaa ggttccacca
      661 agaatgccga cgacaagtag ggccagggca tagacgccga atgtttgaaa tgtttccggc
      721 acagtcagcc ggctcggtgg caaccatccc catccctcaa tcagcccata cagttgaata
      781 ttcgtcaata acccaaacac aataatacag aaacaggcgg taagaagccc cactcgccga
      841 cggggaacga gactggcaac cgctactgtg atgacgtaca gcacagcgaa ggggctctcg
      901 atcccccctg tcctgctcac caataccgtt tcaaggagga agtcgatcgc gacctgggtc
      961 caaaaaaata tggtgagcgt tttcgatgcg ctaagcacgc ggagcaaaag tgcagaaggg
     1021 atcgtcaaga tgtatgtgac aataatcagg gtgtagaatg tctcgacccg ttcgcccttc
     1081 acgacttgga aagcgagaga caggccaagc ataagggtca cgagggccac ccgaagcccc
     1141 atgatccaat acagtcttgt acgcaaatcg ttcatgagca gaataccact gggcgagata
     1201 aggaagcggc gggacaggca gagcgatctg cctcgttcat cctaccagga ttagcgggca
     1261 ggacctgtgc catcaatgca cttgtgatgc ggcgccgggc ggtttgggca gaaagactta
     1321 gcgccttcat gctgggaact agatccaact ccgtgagagg aatggacgaa acactagctc
     1381 tatccgattg cggacgccat tttgaagatc gggagataca tcgcaatcac aataaaccca
     1441 atcacaatcc ccaagaagac catcatcgct ggttcaagca gtgcggtcaa cgatgcgact
     1501 gcctgatcaa cttcgtcttc gtaaaagtct gcaattttcc ccaacatggc atccagcgcg
     1561 ccggttgatt cgccgacggc gatcatatgt gtcaccatct ttggaaacac cccgctcgcc
     1621 gccaagggat cggcgatggt ctttccacca ctgatactga ggcgagcgtc gagcaaggtt
     1681 ccttcaacca ctttattgcc tgacgtcttg gcacagatgg tcatgccatc gagtaagggg
     1741 actccgctag agagcaatgt cccgagcgta cgggtaaact tggcaaccga ggcttttctg
     1801 atcaagtctc caatcactgg tatcctaaga agcaacttat cgatgaccag ccgccctcga
     1861 acggtcccat agtacttttt aatcccaacg atgatcccca caatagcccc aagaatgaca
     1921 taccagtacc cgataaaaaa gttactgatg tcgatgacca gttgcgtagg gccgggcaac
     1981 gcgactcttc cacctgatag ttcaaggaac atcttggcaa agattggaat gacaaacagc
     2041 atgagaatcg tgatcacagc aacggcaact cccatgatcg tagcgggata gaccatggca
     2101 gccttgatct gccctttgag cttcatcgct ttctcgatgt attgtgcgag acgcgtcaag
     2161 atcgtatcga gcaggccgcc cacctcgccc gcatgcacca agttgacgta cagatcgtca
     2221 aagatttttg gaaaacgccg taaggcatcc gagaatgttg aacctgcctc aacctgaatc
     2281 ttcacttctc cgatcgtctc tcgcagcact ttattctcgg actgagtcga gaggatttcg
     2341 agacactgta ccaagggcag acctgcattg atcatcgtgc caaactgtcg cgtaaataca
     2401 accaggtctt tatcggtgag accagaccca aaactcagct tgaagccacc cccgccaccc
     2461 ttcttttctt caaggctggt gacgacgacg ctttgcttgc ggagttgatc gaccgcctca
     2521 tctcgggttt ttgcactcag ctcgcccttc ttgacggcac ctgatctggt tcgtccgaca
     2581 tacgcaaagg tagccatgcg cgacactcac ccttcgatgt ggccatcacc ttggccggtt
     2641 gtagactgag aggaatacct accaggcgag tctactggcc accccaaaac ctgtcaaaca
     2701 aatgcacaat ctctgcgcag gcgatccggc acacaggccc aagtaataca gcgccttagc
     2761 cgccgatggt actcgtgtgc cgaatgcctc ggtagagata gtgcaagccg gacaatagcg
     2821 tgaaggcaac catgccgaac aagagaggta ggacgagggt gagatcgatc tgacgggaac
     2881 tcaagaatat gaccatgaca acatacgaca gttggagaaa ggtagtgccc ttgcccaaga
     2941 atgtcggggt aagatctat
//
LOCUS       DATIPF010000011         4464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1505071, whole genome shotgun sequence.
ACCESSION   DATIPF010000011 DATIPF010000000
VERSION     DATIPF010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505071"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VLL06_00275"
     CDS             complement(<1..423)
                     /locus_tag="VLL06_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29431.1"
                     /translation="MDISSLIDSLHPLEVKVLSTFGANPSAVLQTEQLAQSSGLEPSQ
                     LSMAVEWLLAKSLLTVDSETVTPNVSLTKIGELYFEKYSPIERVLSAARDAAKTGKRL
                     TIQDIQSREGLEPTEVSGGIGRLKKEGALLIIQGGCIES"
     gene            complement(433..1125)
                     /gene="rpe"
                     /locus_tag="VLL06_00280"
     CDS             complement(433..1125)
                     /gene="rpe"
                     /locus_tag="VLL06_00280"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HSS29432.1"
                     /translation="MARIAPSILAADFARLAEEVAAVERAGADFLHIDVMDGHFVPNL
                     TIGPPIVAAIRKVTKLPLDVHLMITNTDTFIAEFAEAGADYLTVHVEACPHLHRTVQS
                     IKERGVKAGVTLNPATSLSTIEEILPDVDVVLIMSVNPGFGGQTFIPSCLRKISSARA
                     MIDRAGSHALLEVDGGVKIDNAAQVLAAGADVLVAGSAIFSSRDYAATIAALRAAGQP
                     SSRTPKRVSAHR"
     gene            complement(1125..2564)
                     /gene="rsmB"
                     /locus_tag="VLL06_00285"
     CDS             complement(1125..2564)
                     /gene="rsmB"
                     /locus_tag="VLL06_00285"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HSS29433.1"
                     /translation="MASGSRSLLTAKTATSARSIALLLLVESVKCEEGVDVLLDRALT
                     RCSFDSRERALTVELTYGVLRRLATIDWRLEPVLDKPLLRLPVAVQMVLRLGAYQLLF
                     LDRIPESAAVNESVNLSKACTPTLGRDWSGFVNAVLRALLRHPVQAWPSMDHDAAKAL
                     AVRYSVPDWLSRRWVEHLGIASAEAACEGASIAPPLTLRVNRLITTREALLETFAQAG
                     LAAKPTSVSPCGILLDEGGLVPSLPGFHEGAFYVEDEAAQLIPPLLDPQPDDTVLDAC
                     AAPGGKSTHLADLMHNKGTIYAIDRKGARLDLLRSNCHRLGIRIVVPMVSDIRQPREW
                     ISNIKTTRSSSVKKARAGESVDRILVDAPCSGLGVLRRHPEAKWRKGEQALPRHHTIQ
                     CQILEAVAPCLRPGGVLVYSTCSTEPEENEDVIKEFCYAHTEFQRESVLPWLPPTAQG
                     LVTEQGALSTVGNQCSMDGFYAVRLRKVL"
     gene            complement(2607..3563)
                     /gene="fmt"
                     /locus_tag="VLL06_00290"
     CDS             complement(2607..3563)
                     /gene="fmt"
                     /locus_tag="VLL06_00290"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HSS29434.1"
                     /translation="MRLVFMGTPDFAVASLEALLKSDDSVVGVVTQPDRPKGRGQILT
                     PSPVKLLAKRQQIPLLQPVKMKDPEFLQALAGWKPDLIAVAAFGRILTPTILSLPPRG
                     CINVHGSLLPKYRGAGPIQWAIINGETQTGITTMLMDEGMDTGAMLLQEAISITPDDT
                     ADTLSPRMAALGGRLLVETIARLKAGTLAPQLQDASQVTFAPLLKKEDGVIDWALPAT
                     ALANRIRGLSPWPGAYTTVSGGDRWTIWRARALPGPATKPPGVIVAVTHEAIHVATGE
                     GVLAVMEVQPMNSRRMAVSQYLTGHPIAPGLQFGRTVSFLAP"
     gene            3648..3724
                     /locus_tag="VLL06_00295"
     tRNA            3648..3724
                     /locus_tag="VLL06_00295"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3682..3684,aa:Met,seq:cat)
     gene            3816..>4464
                     /locus_tag="VLL06_00300"
     CDS             3816..>4464
                     /locus_tag="VLL06_00300"
                     /inference="COORDINATES: protein motif:HMM:NF019313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HSS29435.1"
                     /translation="MKRKQRKPKSRIEYLPDSPLTDPARDELSRLPFAVQISNTIASR
                     TDASPLIVGLYGEWGAGKTTLLNFIRGELDKRRNIICFKYNPWRFPDETILIKSFFGI
                     LAAELNRGLGGGKELLGKFVKGLGKLLPSISGSIYGYGVEVDAEKTFTNLGEMLSETN
                     LEQLKTRLEELLQQEQKRVVILMDDIDRMDNSEIQALFRLIKLTADFSYVTYVLAF"
BASE COUNT         1053 a         1217 c         1237 g          957 t
ORIGIN      
        1 cgattcgata cagccgcctt gaatgatgag gagcgcgccc tcttttttga gccgcccgat
       61 tccaccgctg acctcagtcg gttcaagccc ttcccgagat tgaatgtcct gaatggtcag
      121 acgcttgccg gttttggccg catcgcgagc agcggacagc acccgttcga ttggcgagta
      181 cttttcaaaa tagagttcgc cgatttttgt gagcgaaaca tttggggtca cggtctcgga
      241 atcgaccgtg agtagcgact tggcgagcaa ccattcgact gccatactca actgtgaggg
      301 ctctaacccg gacgattgcg ccaactgctc agtctggagt actgcggaag gatttgcccc
      361 aaaggtcgag aggactttga cttcgagggg atgaagacta tcgatgaggg aagaaatgtc
      421 cacccgcgcg gcctatcggt gagcggagac gcgctttggc gtcctggaag agggttgacc
      481 ggctgcgcgt agcgccgcga tcgtcgcggc ataatcccga ctggaaaaaa tggccgatcc
      541 ggcgaccaag acatcggctc ccgcagccag cacttgagct gcattgtcga tcttcacgcc
      601 gccatccact tcgagcaatg cgtgacttcc tgcccgatcg atcatggcac gagccgatga
      661 aatcttccga agacaggacg ggataaatgt ttgaccgccg aagcctggat tgaccgacat
      721 gatcagtacc acatcgacat ccgggagaat ttcctcgatc gtgctgagcg aggtggccgg
      781 attcagagtc actccggcct tgacgcctcg ttccttgatc gattgcaccg tacgatggag
      841 atgcgggcag gcttctacgt ggaccgtaag atagtccgct ccagcttcgg caaactctgc
      901 gataaacgtg tcggtatttg tgatcataag atggacgtcg agcggcaact tggtgacttt
      961 tctgatggcc gccacgatcg gcgggccgat agtcaaattc ggcacaaaat gcccgtccat
     1021 gacatcgata tggaggaagt cggctcctgc gcgttcaacg gctgccactt cctcggccag
     1081 tctggcaaag tcagcagcta agatcgaggg ggcaatacgt gccattacag gaccttcctc
     1141 agtcgtacgg cgtaaaatcc gtccatcgaa cactgattac ccactgtcga gagggcaccc
     1201 tgttcagtga cgagaccctg tgccgtaggc gggagccagg ggaggacgga ctcacgctgg
     1261 aactccgtat gggcataaca gaattctttg atgacgtctt cgttttcttc cggttccgtc
     1321 gagcaagtac tatagaccag caccccgccg ggccgcaagc atggggcaac cgcttcaagg
     1381 atttgacatt ggatcgtgtg atgtcgaggg agtgcctgct cgccttttcg ccacttcgct
     1441 tcaggatgtc gccgtaacac gccgagcccg ctgcagggcg catcaaccaa aatccgatcg
     1501 accgattcgc ccgctctggc tttcttcaca gaggaagatc tagtcgtttt tatgttggaa
     1561 atccattcgc gtggctgacg tatatcgctg accattggca cgacaatcct gataccgagt
     1621 cgatggcaat tgcttcgcaa gagatctagt cgggccccct tccgatcgat agcatagatg
     1681 gtccccttgt tgtgcatgag atcggctaaa tgagtcgatt ttcctcccgg cgccgcacag
     1741 gcatcgagta ccgtatcatc aggctgagga tcgaggagag gaggaattaa ttgcgcggct
     1801 tcatcttcga catagaaggc accctcatga aacccaggga gcgaaggcac gaggcctcct
     1861 tcatcaagca gtatgccgca tggactcaca ctggtcggct tggctgcaag acctgcctgt
     1921 gcgaatgttt cgagcagtgc ttccctcgtc gtgatcagcc ggttcactcg aagtgtcaga
     1981 ggcggcgcga tgctggcccc ctcgcaggcc gcttctgctg acgcgatgcc cagatgttct
     2041 acccatcgac gactgagcca gtctggaacc gaataccgca ccgcaagcgc tttcgccgca
     2101 tcatgatcca tgcttggcca ggcctgaacc ggatggcgca agagcgcacg caagaccgca
     2161 ttgacgaacc cactccagtc cctgccgagt gtgggggtac aggctttgga taggttgacg
     2221 gattcgttga cggcggcaga ctccggaata cgatcgagaa agaggagctg gtaggcgcca
     2281 agccggagca ccatttgcac ggctacggga aggcgaagaa gcggcttgtc cagcaccggc
     2341 tcaagccgcc aatcgatggt cgcaagccga cgcaagacac cataggtgag ctccaccgtg
     2401 agggcccgtt ccctgctgtc gaacgagcat cgcgtgagcg cgcgatccag caacacatcg
     2461 accccctcct cacacttgac cgactccacc aacaacaaga gggcaatcga acgagctgac
     2521 gtcgcggtct tggcagtgag taacgatcga gaaccagagg ccatagggga ctcaggacag
     2581 agaaattccc gttgcgtgat gaacgatcag ggcgctagga aagagacggt cctcccaaac
     2641 tgcaacccag gggcgatcgg atggcctgtc aagtattgag atacggccat gcgtcggcta
     2701 ttcattggct gcacctccat cacggcaagg actccctccc cggtcgcgac gtgaatcgct
     2761 tcgtgtgtga cggcgacgat taccccggga ggctttgtcg caggccctgg aagtgcgcgc
     2821 gcacgccaga tcgtccaacg atcacctccc gatacggttg tgtaggcccc gggccaggga
     2881 gagagtccgc gaatccgatt ggccagagcc gtggcgggca acgcccaatc aatcacacca
     2941 tcttcctttt tcagtaacgg cgcaaaggtc acttgtgaag catcttgcag ttgcggtgcg
     3001 agggtgccag ccttaagcct ggcaatcgtc tcgaccagca gtctgcctcc gagcgcagcc
     3061 atacgtggcg agagggtgtc ggcggtatcg tccggcgtaa tagagatagc ctcttggagc
     3121 aacattgcac cggtatccat cccctcatcc atgagcatgg tcgtaatccc tgtttgcgtt
     3181 tcgccattga taatagccca ctggatcgga ccagcgccgc gatatttggg aagaagggag
     3241 ccgtggacat tgatacagcc gcgtggggga agtgagagga tcgttggtgt caggatgcgc
     3301 ccgaacgccg cgactgcaat caggtccggt ttccacccgg ccagagcctg gagaaattca
     3361 ggatccttca tcttcaccgg ttgcagcaga ggaatctgtt gtcgtttggc cagaagtttc
     3421 accggagacg gcgtgaggat ttgtcctcgc ccttttggtc gatccggttg cgtgaccacg
     3481 ccgacaacgg aatcgtccga cttcaacaag gcttcaagag aggccaccgc aaaatctggc
     3541 gtgcccatga atacaagacg catatgatcg ctttagcatt gcaggtgagc gaaagcaact
     3601 cagagcgttg gcttgacttg ccgttcagcg ctttgttagc atccgcccgc ggggtggagc
     3661 agcccggtag ctcgttgggc tcataaccca aaggtcgaag gttcaaatcc ttcccccgca
     3721 accaagaata ttaaggggtt agctgatagc ggctaacccc ttcttctttt catctaaccg
     3781 ctacctaacg attgttctta aaaagtgaac agtacatgaa aaggaagcaa aggaaaccca
     3841 aatcaagaat cgaatatctt cctgatagcc ctctgacaga tccagcccgc gatgagttaa
     3901 gtcgtctgcc tttcgcagtt cagatttcca atacgattgc ttccagaaca gatgccagcc
     3961 ctctcatagt gggcctttac ggagaatggg gggcaggaaa aactaccctc ttaaatttta
     4021 tcagaggcga actggataag agaagaaaca taatatgttt caaatataac ccatggcggt
     4081 ttccggacga gaccatccta attaaaagct tctttggaat tctcgcagca gaacttaatc
     4141 ggggattagg gggaggcaaa gaacttttag gcaaattcgt gaaaggactt ggaaagctat
     4201 taccttccat atctggatcc atatatggat atggagtgga agtcgatgcg gaaaagactt
     4261 tcacgaacct cggggagatg ctttcggaga caaatcttga gcaattaaag acccgtcttg
     4321 aagaacttct tcaacaagaa caaaaacgtg tcgtcatttt aatggatgac atagacagga
     4381 tggacaattc tgaaatccag gcactattta gactgataaa acttactgcg gacttttcct
     4441 acgtaaccta tgtcttggcg ttcg
//
LOCUS       DATIPF010000012         3871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_708907, whole genome shotgun sequence.
ACCESSION   DATIPF010000012 DATIPF010000000
VERSION     DATIPF010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708907"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..622)
                     /locus_tag="VLL06_00305"
     CDS             complement(95..622)
                     /locus_tag="VLL06_00305"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HSS29436.1"
                     /translation="MWKDSLRVEAYGTLDEVNAVVGVVRVTNAEMVGHHAQAEQLEED
                     LRWIQNKLFDIGGILATAPGQTFKNMPQVSEKDVTKLEKIIDRCQKDLAPLKEFILPG
                     GGKVSGFLHQARTVCRRAERVCVHLSREETVDAVHIKFVNRLSDALFVLARWAAKTQG
                     EPEFLWERDVKKTAG"
     gene            complement(597..1397)
                     /gene="bluB"
                     /locus_tag="VLL06_00310"
     CDS             complement(597..1397)
                     /gene="bluB"
                     /locus_tag="VLL06_00310"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HSS29437.1"
                     /translation="MSPPSDRLSSGNGQFSDHERAAVYRVIFERRDVRRNFVRTPIAD
                     DVLARILTAAHHAGSVGFMQPWDFVIIRQRATKRAVKQLFKKTNAEATGRYEGARATL
                     YRSLKLEGIEEAPVNICVTCSRQRGGPHVLGRSTVRETDLYSTCCAIQNLWLAARAEG
                     VGVGWVSILDHRALKQVLGIPRQVKVLAYLCLGYVSEFAAQPDLETAGWRSRIPVEQL
                     VHYESWGNMIRSDRRRGDAHHESLHENRGRGKNAAGRRAAGVEGQPPS"
     gene            complement(1446..1667)
                     /locus_tag="VLL06_00315"
     CDS             complement(1446..1667)
                     /locus_tag="VLL06_00315"
                     /inference="COORDINATES: protein motif:HMM:NF025733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HSS29438.1"
                     /translation="MRGPVIKTCEHCKQSFECGQYGCWCGRVGITEEQMDWIAARFQD
                     CLCPACLKMVSAGALDVFSVKQTSPTGAE"
     gene            complement(1671..2270)
                     /gene="cobO"
                     /locus_tag="VLL06_00320"
     CDS             complement(1671..2270)
                     /gene="cobO"
                     /locus_tag="VLL06_00320"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HSS29439.1"
                     /translation="MTEQDEHKARMERLKASVDRRIEAAQEEKGLLIVYTGAGKGKTT
                     AALGMALRCIGHGMKVAVVQFIKGAIDTAEERALKSFGGQVIFLRMGEGYTWETQNRE
                     RDTKFARDAWTAACGFLRDPSYAMVILDEFNIALQHDYVRLEDVLPILRGRPPMQHAV
                     ITGRGAKDELIEEADLVTEMKQSKHPFRKGIKAQPGVEY"
     gene            complement(2267..3667)
                     /locus_tag="VLL06_00325"
     CDS             complement(2267..3667)
                     /locus_tag="VLL06_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HSS29440.1"
                     /translation="MNIRRLVIAGTHSGVGKTTVTLAILAALQQRGRQVQAFKAGPDF
                     IDPGHHRMVTGRPSHNLDGWMLGEAVNLDIFTRAAAGADLSIVEGMMGLFDGSSPLNE
                     IGSTAELAKQLDAPVLLVIDGSAMARSAAAMVSGYAKFDPAVRVAGVLFNRVSSEGHY
                     RLLKEAVEQETDVVTVGYLRQDPAMTIAERHLGLSTAMEQRTGELYSCIAKAAAETIN
                     LDQIEALAQMCKELPVKASQPVIRNQGRTVRIGVAQDQAFCFYYHDNLELIEAEGAEL
                     VRFSPMSDRVLPDVDMLYLGGGYPELHGKALADNVSLRTAIRQFAERGGTIYAECGGM
                     MYLTQAIRDFAGTSHEMVGLFPAEAVMQKSSMTLGYRTMSCSRHCILGEAGMTARGHE
                     FHYSTLVPRGPLDYACALRDVRGDSKGSDGLVMGKTLALYTHIHFASQPQLAGALVAS
                     ARGTRMEPSLSSRAKR"
     gene            complement(3677..>3871)
                     /locus_tag="VLL06_00330"
     CDS             complement(3677..>3871)
                     /locus_tag="VLL06_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29441.1"
                     /translation="DVNNLQSMRAFDVWNVFASYDVTNRVQTYIRVDNIFDEKYEEVL
                     LFGTPARSIFAGLKVNYDVK"
BASE COUNT          844 a         1249 c          906 g          872 t
ORIGIN      
        1 agccaaatcg agtgccacat ggctcttacc ggacgctgcg ccgccgagta cgagaatcaa
       61 ctttccacgc cccttacgaa ccttcctcat acgatcagcc tgccgtcttc ttcacgtcgc
      121 gttcccagag aaactctggc tctccttgtg tcttggctgc ccagcgtgcc aggacaaata
      181 aggcatcact caaacgattc acgaatttga tatgcaccgc atcgaccgtc tcttcccgtg
      241 aaagatggac gcagacccgt tctgccctgc ggcataccgt ccttgcctga tgtaaaaatc
      301 ctgacacctt ccccccgcca ggcaaaatga attctttgag cggagccaaa tctttttgac
      361 aacgatcgat gatcttctcg agtttcgtca catctttctc cgaaacctgc ggcatattct
      421 tgaacgtttg gccaggggct gtcgcaagga tccccccaat atcgaagagt ttattctgta
      481 tccatcggag atcttcttcg agctgttcgg cctgcgcatg atgcccgacc atctcggcat
      541 tcgtcacccg caccactcca accaccgcat tcacctcatc gagcgtaccg taggcctcaa
      601 ctcggaggct gtccttccac acctgctgcc ctccggccag ccgcgttttt cccgcgtccc
      661 cggttctcgt gtagactttc gtgatgcgca tcgcctctcc tcctgtcgct ccgtatcata
      721 ttgccccacg attcataatg aacaagttgt tcaacgggaa tcctgctccg ccatcctgcc
      781 gtttccagat cgggctgggc ggcaaactcg gagacatagc cgagacagag ataggccagg
      841 actttgactt gcctggggat gccgaggacc tgtttcaatg ctctgtggtc cagaatactg
      901 acccatccga ccccgacacc ttccgctcgt gctgccaacc agagattctg aatcgcacaa
      961 caggtgctgt acaaatccgt ctcgcgcaca gtggagcgcc ccagtacatg cgggccgccg
     1021 cgctggcggc tgcaggtcac acaaatattg accggcgctt cctcgatgcc ttcgagcttc
     1081 aaactccggt agagggttgc gcgagccccc tcataacgcc cagtcgcctc tgcattcgtc
     1141 tttttgaaga gctgtttcac ggcgcgcttc gtcgcgcgtt gacggatgat cacaaagtcc
     1201 cacggttgca taaacccaac agacccggcg tgatgcgctg cggtcaagat tctcgcaagc
     1261 acatcatccg cgatcggcgt ccgcacaaaa ttgcgtcgga cgtcccgccg ctcgaaaatc
     1321 acgcggtaga cggctgcccg ctcatggtcg gagaactgtc cattaccgga actcagcctg
     1381 tcggacggcg gactcatacc acgctccccg catataggtt catgaagatg atcatgggat
     1441 ggccttcact cggcgcctgt cggactcgtc tgcttgacgg aaaacacatc gagtgcacca
     1501 gcactgacca tcttcaaaca ggcaggacag agacaatctt ggaaacgagc ggcaatccag
     1561 tccatctgct cttcggtaat ccccactcgc ccgcaccagc aaccgtattg gccacattca
     1621 aaggactgct tgcaatgctc gcacgttttg atgaccggcc ctctcatggc ttagtactcg
     1681 actcccggtt gggccttaat gccctttcga aacggatgtt tagattgctt catctccgtg
     1741 acgagatcgg cttcctcgat caattcgtct ttggctccgc gaccggtaat caccgcatgc
     1801 tgcataggag gcctgccacg gagaataggc agcacgtctt caagccgtac atagtcatgc
     1861 tgcagcgcga tattgaactc gtcgagaatg accatcgcat acgagggatc gcgtagaaac
     1921 ccacaggctg ccgtccaagc atcccgtgca aacttggtat cccgctcacg attttgtgtt
     1981 tcccaggtat agccctctcc catccggaga aagatcacct gaccgccgaa cgacttcaac
     2041 gcccgttctt ctgctgtgtc gatggctccc ttgatgaact gcaccactgc caccttcatc
     2101 ccatgaccga tgcaacgcaa cgccatcccc agcgcagcgg tggtctttcc cttgccggca
     2161 ccggtataga cgatcaacaa ccctttctct tcttgcgccg cctcaatgcg cctgtccacc
     2221 gacgccttca accgttccat cctggctttg tgttcgtcct gctccgtcat ctcttcgctc
     2281 tggaactcaa agatggctcc atacgagtcc cgcgcgccga agcgaccagt gccccggcca
     2341 attgtggctg actggcgaaa tgaatgtgcg tatagagtgc cagcgtcttg cccatcacga
     2401 gcccatccga gcccttcgaa tcccctcgca catcacggag tgcacaagca tagtcgagcg
     2461 gtcctctcgg caccagcgtc gaataatgaa actcatgccc cctagcagtc atgccggctt
     2521 caccgaggat acagtgccga gaacagctca tggtccgata ccccaaggtc atcgatgatt
     2581 tctgcatgac ggcttcggca ggaaatagcc cgaccatctc atgcgatgta ccagcgaagt
     2641 cgcggatagc ttgcgtgaga tacatcatcc cgccgcattc tgcgtagatc gtcccacccc
     2701 gctcagcgaa ctgccggatt gccgttcgca ggctgacatt atcagccagc gccttgccgt
     2761 gcagttccgg atagcccccg ccgaggtaca acatatccac atctggcaat acccgatcgc
     2821 tcatgggcga aaacctcacc aattcggccc cctctgcttc aatcaactca agattgtcat
     2881 gatagtaaaa acaaaaggcc tgatcctgcg ccacaccgat tcgtactgtc cgaccttgat
     2941 tccttatcac gggctgagag gccttcactg gaagctcttt acacatctga gccaacgctt
     3001 caatttgatc cagattgatc gtctcagccg ctgccttggc tatacagcta tagagttcgc
     3061 ctgtcctttg ttccattgca gttgagagcc cgagatggcg ttcagcaatc gtcatcgccg
     3121 gatcctggcg caaatacccc accgtcacca cgtcggtctc ttgctccact gcttccttga
     3181 gaagcctgta atgcccctcg ctactgaccc gattgaagag tacccctgcc acacgaactg
     3241 ctggatcgaa cttcgcatac cccgacacca tggctgcagc ggagcgagcc atggcacttc
     3301 catcgatgac gagcaaaacc ggcgcatcca attgcttcgc caactcggcc gtactgccaa
     3361 tctcgttgag cggcgaactg ccgtcgaaca ggcccatcat gccttcgaca atagagagat
     3421 ccgcaccggc agcggctcgc gtaaagatgt cgagattgac cgcctcgccc aacatccagc
     3481 cgtcaaggtt atgtgaaggc cgtccggtca ccatgcggtg gtgaccggga tcgataaagt
     3541 caggtcctgc tttgaacgcc tgcacctgac ggcctcgttg ttgcagcgcc gccagaatcg
     3601 ccagggtcac cgtcgtctta ccgacaccgc tatgggtgcc ggctatgacc aatcgtcgaa
     3661 tgttcatgta attctattac tttacgtcgt aattcacttt caacccagca aagatggacc
     3721 gtgcaggggt accaaaaagc agaacctctt catacttttc atcaaagata ttgtccactc
     3781 gaatataagt ctgcacgcga ttcgtcacat cataactggc gaatacattc catacatcga
     3841 atgcacgcat tgactgcaga ttattgacgt c
//
LOCUS       DATIPF010000013         5773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_443974, whole genome shotgun sequence.
ACCESSION   DATIPF010000013 DATIPF010000000
VERSION     DATIPF010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443974"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VLL06_00335"
     CDS             <1..217
                     /locus_tag="VLL06_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29442.1"
                     /translation="CFRLDTSYDQLVVAPIMTNLDAPGGGTDYAKTPKGNLDISGKYF
                     IWTSNLGGNRLDAFLVKVPSQLLVTGE"
     gene            complement(414..788)
                     /locus_tag="VLL06_00340"
     CDS             complement(414..788)
                     /locus_tag="VLL06_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS29443.1"
                     /translation="MAKVLVIDDEHNFRTILEMVLRSHGYEVLLADCGTTGLSIYQRE
                     HPAVVVLDLKMPGMDGIAVFQEIRHENPKQPVIVLSGDSSPETEREVRALGVSEFIVK
                     GLSMHILTDTLKRLLASLSHAA"
     gene            complement(1098..1310)
                     /locus_tag="VLL06_00345"
     CDS             complement(1098..1310)
                     /locus_tag="VLL06_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29444.1"
                     /translation="METPVIREEVQAYLQACLAFAEFSRYNDLTTGEREAVGNLSQAL
                     GLNLHRTLSPDDPPLAATLSNLPPID"
     gene            2416..2796
                     /locus_tag="VLL06_00350"
     CDS             2416..2796
                     /locus_tag="VLL06_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29445.1"
                     /translation="MEEMVIVEEEGASSALQITLGDFLIFIKWMNQTEKSLDRGEALP
                     TMPDLDSDKVKGWIQLVQRLEERYRWSEGKKAALEANVIAHEAQMGQLVSHLHANIAA
                     LYDNLGQSQKAQEVRRRAEALHIG"
     gene            complement(2895..3578)
                     /locus_tag="VLL06_00355"
     CDS             complement(2895..3578)
                     /locus_tag="VLL06_00355"
                     /inference="COORDINATES: protein motif:HMM:NF016206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HSS29446.1"
                     /translation="MLIALLLIIAGIVIFGPQLWTRRVFAQYRVPRPDYPGTGGELAR
                     HLLDRLDMKHIKVETTEKGDHYDPETKTVRLTPDNFSGKSLTAVTVAAHEVGHAIQDH
                     MGYQPLTERTRLVRFAQGAEKLGAVVMIGIPIATALTRTPAAGVLVLVAGMATMGIST
                     LVHLITLPVEWDASFRRALPVLQQGNYLSPDDMQGARRILIAAALTYVAASLASLLNL
                     WRWIAFLRR"
     gene            complement(3855..5075)
                     /locus_tag="VLL06_00360"
     CDS             complement(3855..5075)
                     /locus_tag="VLL06_00360"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HSS29447.1"
                     /translation="MNWIHSWSGLLFGWLLFAVFLTGTLTVFDNEITYWMQPELLEVS
                     SDARDGDETWPPVLQFAAQANHWEAVVHTDRPPLLMVKLQKNRTFSGQATDPATGKIV
                     TFRDTEGGDFFYHFHHGLLLGVPGAWIVGTAGMVMLVTLVSGLGIHRWNIKDMFVVRP
                     WSLPQRAWFDAHNLTGILALPFHLMITFTGLMIFWSIYMPKEVQFLYGGDFSLSLLSD
                     LHFAQFGGTALRWFYFLMGLAATAMIATGLVLWTNKRRKYHAERSGVTRFRFVEALNV
                     ATVAGLLVAIAAFFWANRVLPLALIDRSQWEMRCFFVIWCCCFAHSLLRGGSIFAWKD
                     QLYAAAFLLGLLPLLNGLMTNSHLLMTLPKGQWALAGFDLTALAAGLLLGWTARRVGK
                     AAPDISKLSRTEKP"
     gene            5397..>5773
                     /locus_tag="VLL06_00365"
     CDS             5397..>5773
                     /locus_tag="VLL06_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29448.1"
                     /translation="MGWIRWYSIVLVMSYGLGCLGFFGPAAEAADTVSWKSYTNSRYG
                     FSAQYPSDWRPGNPMPDGAGITLSLPIEGGQVTLSGFMNVIGGSSQDGRQTLDEFVMA
                     HRRIITGLYEKKHIKVLWEKDHPT"
BASE COUNT         1379 a         1537 c         1642 g         1215 t
ORIGIN      
        1 ctgctttcga ctcgacacga gctatgacca actggtcgtg gccccgatca tgacgaacct
       61 ggatgcgccc ggcgggggaa ccgattatgc caagactccg aaggggaacc tggatatcag
      121 cggcaagtat tttatttgga ctagcaacct cggcgggaat cggctcgatg ccttcttggt
      181 caaggtgccc tcccaattac ttgtgacggg agagtgacgg ctcccatagt gggggtggag
      241 aatgcggact tgccgcagga cgctgggcac cttcatgtcg gaattctcag atccagcacg
      301 acggcatcag ggcatgcgga gcgaaagagt tcgagtcctt ttcggcgact ctcagcgaac
      361 atcacatggt agcagccatg ctgactgagt cagagaggtt atgggattag ccgttatgct
      421 gcatgactga gggatgcgag caagcgcttg agcgtgtctg tcagtatatg catcgagagg
      481 cccttgacga taaactcgct gaccccgaga gcacggacct cccgctccgt ctcgggggaa
      541 ctgtctccac tcaacacgat caccggctgt ttaggattct catggcgtat ctcttggaag
      601 accgcgattc catccatccc cggcattttc agatcaagca ccacgacagc ggggtgttct
      661 cgttgataaa tggataggcc cgtcgtgcca cagtcagcaa gtagcacctc atatccatgt
      721 gaacgaagta ccatttcaag aatcgttcgg aagttgtgct catcgtcaat caccaggacc
      781 ttcgccattg ccctctcctg atgtcgttcc gaaacgcacc cattttatcc tactaataca
      841 atgagagaat gcgcctagga aaagccctaa tgtcgccccg cagcggcgtg tcagtcgatc
      901 agggacagga atggtgaggg gctcggcctg gcaggcggcg atagctttcg gcgggaaaga
      961 cggggattgt cataggcgtc cacgaattcg gcgaacgccg acacggggaa aaggaacagt
     1021 gcgaactgga tgaacggacg catgcagcgg ctcagggggg gcaaatctgc tgagctagca
     1081 gagagcagga tgaggcatca gtctatcggt ggaaggttgg aaagcgtcgc cgccaagggt
     1141 ggatcatctg gagacagcgt gcgatggaga ttcaatccta gcgcttgaga aaggtttcct
     1201 acggcctcgc gctcaccggt cgtcaagtcg ttatatcgag aaaattccgc aaaggccagg
     1261 caggcctgca gatacgcttg aacttcttct cgtatgacgg gtgtttccat gactcaggct
     1321 ctcacacagt ccaaatatac gggactagga aggtacccag ttgggtcatt taggtcgctt
     1381 cgtggagtgg gagttcgtcc tcccccgatg aatgggggaa aaaggactgg ggtttttcct
     1441 cgtggggatc gtttctgagt tgacgtatta tgaagttgcg tcagaaactc gtgatggaaa
     1501 aactgcatac gcgcgatgga aacattgcaa acgagcagtg gaagcatcac gaagtctgag
     1561 gcagaaggct cgaatccgaa aggaatcggg cctttgtttt gataacgaaa ctatcctcgg
     1621 tgccatcggt tggtaagcgt aaggagagga ggtcctcagc acggcgggcg agcgcacgac
     1681 cacctcgcgc agcccgttgt ggtcgtcccc atgccccctc cgggtttctc gctgaacact
     1741 cagcactcca aagcagcacc ctccttagcc agtcgtacaa tccatgacaa tgctggcggt
     1801 atcaattcga caagagaatc ctagttggat cgatagggct tcggccgcaa ctcgactata
     1861 accccccata gacgagcaac gaacacatct cacagtgacg atgcactctc cttaaaaact
     1921 tatcgagacg acagggcggg ggaatcgcca gtctccatcc caccaaggag gtggatgcga
     1981 tggcacagac atgtatcccc agcattgact ccgtcaattg ccacgaagtg accagtctgg
     2041 tgtcatgcac cctcgatggt ttgcttcaga ggttgccggc ctattcgtgg gcacaagtgt
     2101 tcgcggcggt cgatcggctc agccggaaag gcacgctgat gctcagccgc acgagtcggt
     2161 tcggctacgt gctgctatca gtccgatcct tccgtcccca gtgttcgaaa cacatcagga
     2221 gctggcgttg tgtaggcggg acggccgggt caacccttgt tgttcgggcc gttcctccgt
     2281 cggtcgcttg cgttgatctt aatatgttgt tcctgtttca aaggcggata agacaatctc
     2341 agtggcaatt tggtagacgt atttcgttcg tctgccacac actctcttca acaatttgcg
     2401 tcacaaggag ttgttatgga agaaatggtt atcgtagaag aagaaggtgc ctcatcagca
     2461 cttcagatta cacttggtga ttttctcatc ttcatcaagt ggatgaatca aactgaaaaa
     2521 tctctcgatc gcggtgaagc gttgccgacc atgccggatt tggattccga taaggtcaaa
     2581 ggctggatcc agctcgtaca gcggcttgaa gaacgatacc gttggagtga aggaaagaaa
     2641 gcggcgctcg aagcgaacgt gatcgcgcac gaagcacaga tgggacaact cgtgtcgcat
     2701 ttacatgcga atatcgccgc gctctacgac aacctcggac aatcgcaaaa ggcccaagag
     2761 gtgcgtcgcc gagctgaagc actccatata ggttgacgcc aggcgccttt agtccagatc
     2821 gtcggcggat cgtgcaactc ggagtcttca ttcgccacat tcgtgtacgc gacgggtctg
     2881 tgatgtggcg gtctctatcg gcgaaggaag gcgatccacc gccagaggtt gagcagactc
     2941 gcgagagagg cagccacgta tgtcagcgcg gcggcgatga ggatgcggcg ggctccctgc
     3001 atgtcgtcag gcgacaggta attgccttgc tggagcaccg gaagcgcacg cctgaagctg
     3061 gcgtcccact cgactggaag tgtgatgagg tgcacaaggg tggagatccc catcgtcgcc
     3121 atgccggcaa ccaggacgag tacgcctgcc gcaggggttc gagtcaacgc tgttgcgatc
     3181 ggaatgccta tcatgaccac cgccccgagc ttttccgctc cctgcgcgaa ccgcacgagc
     3241 cttgtgcgct ccgtgagcgg ctggtagccc atatggtctt gaatcgcatg cccaacttcg
     3301 tgggcggcga cagtgacggc tgtgagcgat ttcccactga agttgtcagg ggtcaggcgg
     3361 acagttttgg tctctggatc ataatggtcg cccttctcgg tcgtctccac cttgatgtgt
     3421 ttcatgtcga gccgatcgag taggtgtcga gccagctccc ccccagttcc tggatagtca
     3481 ggacgaggca cacgatattg ggcgaacacc cgcctggtcc agagttgtgg accgaaaatg
     3541 acaataccgg caatgatcaa cagtagggct atgagcatcc gattccctta gcctctccct
     3601 tgtcggacga ggccctcggc cgaggcgaca cacagaatgt tgagatccat cccttcgtct
     3661 ttaacgctaa cacaagggcg catgaaacag tggaataaag gggctgctca acagtattcg
     3721 tgacgtaagg actgtgcgtt gagaacggtg ccgacatatt cccaaggatc catgaatgcg
     3781 ggcaggtcat gagatttcga gaattgaaag gattgagttg agctgatcag taaatcacgg
     3841 gagtgtccgg gcgctcatgg cttttcagtg cggctaagtt tggatatgtc tggcgcggct
     3901 tttccgacgc gtcgggccgt ccagcccagc agcaaccctg cagccaatgc tgtgagatcg
     3961 aaccccgcca gcgcccactg gcctttcggc agggtcatca acagatgact gttggtcatc
     4021 agcccattca gcaagggcag caatcccaat aagaaggcgg ctgcgtacag ctgatccttc
     4081 caggcgaaga tggatccccc tcgcagaagg ctatgggcaa aacagcagca ccagatgaca
     4141 aaaaaacatc gcatctccca ttgcgaccga tcaatgaggg ccaacggcaa cacacgattg
     4201 gcccaaaaga atgcagcaat ggccaccagc aatccagcca ccgtggccac attgagggct
     4261 tctacaaaac gaaagcgtgt cacacctgaa cgttcagcat gatatttcct ccgcttgttg
     4321 gtccacaaca ctagacctgt ggcaatcatg gcagtggcgg ccaaccccat gaggaaatag
     4381 aaccagcgca gagccgtgcc tccgaattgg gcgaagtgca gatcggatag cagcgagagg
     4441 ctgaaatctc cgccatacag aaattggacc tccttcggca tataaatcga ccaaaaaatc
     4501 atcaggccgg taaaggtgat catcaaatga aacgggagcg cgaggatacc ggtgaggttg
     4561 tgagcatcga accaggcgcg ttgagggagg gaccagggtc ggaccacaaa catatcctta
     4621 atattccagc gatgaatacc gaggccgctg accaacgtga cgagcatgac cattccagcg
     4681 gtgcccacga tccatgctcc tgggacacct aagagcaatc catggtgaaa gtgataaaag
     4741 aagtcgccgc cctcagtatc tctgaatgtg acaatcttac cggtagctgg gtcggtcgct
     4801 tggcccgaga aggtccgatt cttttgcaat ttgaccatca gaagtggtgg gcggtcggta
     4861 tgaacaactg cttcccagtg attagcctgg gcggcgaact gcaagacagg cggccaggtt
     4921 tcgtcaccat cacgcgcatc tgatgaaacc tcgagtaact ccggttgcat ccaataggtg
     4981 atttcattgt caaagacggt cagcgtcccg gtaagaaaga cggcgaataa gagccagccg
     5041 aagaggagac cgctccacga atgtatccaa ttcatcaatt gtgtgagttg tccttcatga
     5101 acggcacgtg ccgattaaga acaccgtcct gccgatctcc ggccctcata gacgggcagc
     5161 accgcatcac gtcttggagt gagtcaaaga ccgatccatc gagcggattg cgaatgtcca
     5221 gcatgcgctc ggtcgaacgc aactcaccgg gcggtagctg ttcatgaaag gtagtccatt
     5281 ctttcaaaat caacaaggcc ccccggattc ttttcacccc aggcctatat tccaatcagc
     5341 cgagccgtcc agtatagttc catctgtacc tacgccggaa tcaggaaagg atggagatgg
     5401 ggtggattcg ttggtattca atcgtcttgg tgatgagcta tgggctggga tgtctggggt
     5461 tcttcgggcc tgctgcagag gcagccgata cagtcagctg gaagagctat acgaatagcc
     5521 ggtatggttt ctcggcgcag tatccctcgg actggcgtcc cggcaaccct atgccggatg
     5581 gggctgggat tacgctctct ctccccatag agggtggtca agtgaccctt tccggtttca
     5641 tgaacgtcat cggaggaagc agccaggatg gccgccaaac cctcgatgaa ttcgtcatgg
     5701 cccaccggcg catcatcact ggactgtacg agaagaagca catcaaagta ttgtgggaga
     5761 aggatcaccc cac
//
LOCUS       DATIPF010000014         4890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1239926, whole genome shotgun sequence.
ACCESSION   DATIPF010000014 DATIPF010000000
VERSION     DATIPF010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239926"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..692)
                     /locus_tag="VLL06_00370"
     CDS             complement(318..692)
                     /locus_tag="VLL06_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS29449.1"
                     /translation="MAKILVIDDEAGIRDLLDTLLRRKGYDVVLAESGQKGLDLFRRE
                     RPDVIVLDLKMPGMDGLSVLQQVRSLDPKKPVIILTGAGTAETEQKVRALGVTEYVEK
                     EFSLHLLGDSLKRLLNNPIPST"
     gene            1160..2539
                     /locus_tag="VLL06_00375"
     CDS             1160..2539
                     /locus_tag="VLL06_00375"
                     /inference="COORDINATES: protein motif:HMM:NF023330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 family protein"
                     /protein_id="HSS29450.1"
                     /translation="MAEAEIAKPDTGDLMRKIHERPQMPLAEWLRAPSHVHYKAFRMS
                     DPPVQRPASREEFRSLLEAFKVPSEAMAIRDDFGYGVKVDATGDRMIMIWEAHTEYYS
                     HQTWHIPSGHAEKITFGQLTFPQSRPPISLVGVEVCRLDILLMTEPLPASDVIRPQLP
                     GPVMYGSRILDEETALVTSFTPDEHGRERYWVSVGSGRSSRSHLKDIVDAVVRIETYY
                     HLLLMQKPLFMAAVDQAYKFEQVHLKQREIITGHIGHADSLTLQRWLNGLTQDLLKTT
                     RLAGGLHFELSSSVPYDKIVHRTLASLDERALPPYHPVSDYVLSGITGVAEGYQQLLK
                     RIDTLRSGFEGVISIIRARVDLMLQSQNLTLLASVDKTTKSQAILQYTVEGLSVIVIA
                     YYLSGLMGYIFNGLQEIGWLRSADMASALFVPVSLGIALGITILGRTLLRKKFSGESM
                     PAASEKPQP"
     gene            2606..3625
                     /gene="galE"
                     /locus_tag="VLL06_00380"
     CDS             2606..3625
                     /gene="galE"
                     /locus_tag="VLL06_00380"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HSS29451.1"
                     /translation="MIFVTGGAGYIGSHTCVELLDAGCDVTVFDNFSNSHPEALARVD
                     RITGKKLHLVRGDCRDRAALVAALRQSGASAVMHFAGLKAVGESVAQPLAYYDNNVAG
                     TLRLLEAMGECGVRILVFSSSATVYGDPIRLPITEDHPLFPTNPYGRTKLIVEEILRD
                     LHRSDASWRIGLLRYFNPVGAHVSGLIGEDPQGIPNNLLPFVAQVAVGRRPFLNVWGH
                     DYPTPDGTGVRDYIHVVDLALGHVKALKALEQPQERAECLTVNLGTGTGYSVLDIVRA
                     FEQASGRSVRYKIAPRRPGDIASCYADPQRAIELLGWRAERGLSTMCSDAWRWQHTNP
                     DGYTG"
     gene            complement(3613..3945)
                     /locus_tag="VLL06_00385"
     CDS             complement(3613..3945)
                     /locus_tag="VLL06_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29452.1"
                     /translation="MDEQEPAQVVELRISYRYATAHPWVVQAIGGFLSAYFMEHPGFR
                     VQRHIEELESGTHLWVCEVPSTMKFPRLLKRLKEDIPPCLAKEIVIDLPARPRYLIDS
                     PSDESQPV"
     gene            4100..>4890
                     /locus_tag="VLL06_00390"
     CDS             4100..>4890
                     /locus_tag="VLL06_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HSS29453.1"
                     /translation="MNIAIIGGGPAGLMAAESARAAGAQVDLYDAMASVGRKFLLAGK
                     GGLNLTHSEPSLKFLSRYGERRAQIALLLSSFGPDALRAWTSGLGIGTFVGSSGRVFP
                     TDLKAAPLLRAWLRRLRQAGVRFHMRHRWSGWDEQGALYFSTPEGARSAQADAVVLAL
                     GGGSWPKLGSDASWVSVLAKRGLHIAPLLPSNCGFDVGWSEHFRAKFAGQPVKSVAVV
                     IRNAAGAESWHPGEFVITETGVEGGVIYTVSAFLRNEILAKGMAT"
BASE COUNT         1026 a         1271 c         1425 g         1168 t
ORIGIN      
        1 tcaaaaatgg ccattctttt cgcgtctctg attgcaggaa ccattggatt tctctggctc
       61 agatgttggg ggaggccgga aagacggctc acacgatgat tctgatgcgc tcgtgggatg
      121 tctggttttg gaacgtagga agaccgggga aggtagcgaa gccgttttgg gaacatgccg
      181 cgcgctacgg tttcaatgag caggatattg atacactcga aaggatgaag gggagacgtg
      241 aagcggctaa tccgagagga agtggagcgc agaccgatgc cgtgacgtcg ccacttggcc
      301 ggcgacagaa gcatccatta tgttgaagga atgggattgt tgagaaggcg cttcagagag
      361 tcccccagga gatgcagcga aaattctttt tcgacgtatt cggtcactcc aagtgctcga
      421 accttctgct ccgtctctgc ggttcctgcc ccagtcaaaa tgatgaccgg ttttttagga
      481 tcgagactac gcacctgttg caagaccgag agtccatcca tcccgggcat tttcagatca
      541 agcacaatga catctggacg ttctcgtcga aaaagatcca gacccttctg accactctca
      601 gcgagtacca cgtcatatcc ctttcgacga agtagtgtgt cgaggagatc acggataccc
      661 gcctcatcat caatcaccag aatcttggcc atggtaccac ctcccagaat aagaacaaga
      721 tggcaactcc aagcatatca taataggctg ttctgggaag atctgctaaa atggccacct
      781 cagaactacg taggagtctt ggtttggata gggccagttg gatctgttag gcctacctct
      841 ggtatgcgcc tgggaacgta aaccagataa ggcccatctc ttctggtact ggtagtggat
      901 ggaacccaaa caaaggcgcc ggaaggtttc gttttacggc tttcagatcc ctaggtaggt
      961 gttggacgac ttgcaatctg ataggtggtt cgaggggacg cgattcccgc ctgataccac
     1021 cagcaacgct gactatccgg gtatcatggt acacaatgtc cctcattgca tattcgctgg
     1081 cgtttgattg acagcgttat tggtaagaca gtaatttgga acggtttccc attccgtatt
     1141 caccccttag gattttcgta tggccgaggc agagatagct aagccggata ctggcgactt
     1201 gatgcggaag attcatgagc ggccacagat gcctttggca gagtggttgc gcgctccttc
     1261 gcacgtgcac tataaggcgt ttcgcatgag cgacccgccg gtgcagcgcc cggcgagtcg
     1321 tgaagagttc agatctctcc tggaggcctt caaggttcct tcggaagcga tggctattcg
     1381 tgacgacttt gggtatggcg tgaaagtgga cgcgactggt gatcggatga ttatgatctg
     1441 ggaagctcac acagagtact acagccatca aacctggcat attccatctg gccacgctga
     1501 gaagattact tttggtcagc tgactttccc gcagtctcgg cctccgatca gcctggttgg
     1561 cgtggaagtg tgccgtctgg atatcctgct catgactgag ccgttgccgg ctagtgacgt
     1621 gatacggccg cagcttcctg gtccggtgat gtatggcagc cgaattctcg atgaagagac
     1681 ggcgctggtc accagcttta cacctgatga acatgggcgc gagcgctatt gggtcagtgt
     1741 cgggtccggc agatccagca ggtcgcacct gaaagacatc gtcgatgcgg tggtcaggat
     1801 tgaaacctac taccacttac tgctcatgca gaaaccgtta tttatggcgg cagtcgatca
     1861 ggcctacaag tttgagcagg tacacttaaa gcagcgtgaa atcatcaccg gacatattgg
     1921 tcacgccgat tccttgacct tacagcgttg gctcaatggg ctcacacaag acttgttgaa
     1981 aaccactcga ttggctggcg gattgcattt cgagctgtcc tcctctgttc cgtacgacaa
     2041 gatcgtgcac cgaacactgg cgtcactcga cgaacgcgca ctccccccgt accatccggt
     2101 gtcggactac gtcctgagcg gaattaccgg agtggcggaa ggctatcaac agcttctgaa
     2161 gcgaatcgac actctccgca gcggttttga aggagttatc tccatcattc gcgcccgcgt
     2221 cgatctcatg ctccaatctc agaatctgac gctcttggcc agcgttgaca agacgacgaa
     2281 aagtcaggcc attctccagt acacagtcga aggcctctcg gttattgtga tcgcctacta
     2341 tctcagcggg ctcatgggat atatcttcaa tgggctccaa gaaatcgggt ggctccgcag
     2401 tgcggatatg gcttccgctt tgttcgttcc ggtttcgctc ggtatcgctt tagggattac
     2461 catccttgga cgaacacttc tccgcaagaa attctctggc gaatccatgc ctgccgcatc
     2521 ggagaagcct cagccctgac agaatgctga acaaacacgt gggcgcaaaa agtgttttgt
     2581 ttcgtttgtc ttgagatcgg tgtccatgat ttttgtcaca ggcggtgcag gctacatcgg
     2641 ttcacatacc tgcgtggaac tgctcgacgc aggctgtgac gtgacggtgt ttgacaattt
     2701 ctccaacagc catcctgaag cactggcacg ggtcgatcga atcacgggga aaaagttgca
     2761 tctggtgcga ggtgactgca gagatcgtgc agcgttggtt gcagccctgc gccagagtgg
     2821 agccagtgcg gtcatgcact tcgccggtct caaggcggtg ggggagtcgg tggcacagcc
     2881 gttggcctac tatgacaaca acgtggccgg cactctgcgc ctgctggagg cgatgggaga
     2941 atgtggagtg aggatcttgg tgttcagctc ttctgccacg gtctatggcg acccgattcg
     3001 attgccgatt acggaggatc atccgctctt tcccaccaat ccctatggca ggacgaagct
     3061 aatcgttgag gagatcttgc gtgatctgca ccgcagtgat gcctcctggc gtatcggcct
     3121 tctgcgttat ttcaatccgg tgggagccca cgtgagcggc ttaatcgggg aagatcccca
     3181 gggcattccc aataacttac taccttttgt cgcgcaggtg gcagtggggc gacgaccatt
     3241 tctcaacgtg tggggtcatg attatcccac tccagatggg actggcgtac gggattatat
     3301 ccacgtggtg gatttggctc tgggtcacgt gaaagcactg aaagctctgg agcaaccaca
     3361 ggaaagggcc gagtgcctga ccgtaaactt gggcacgggt acaggttaca gtgtgctgga
     3421 tatcgtgcgg gcatttgagc aggcaagtgg gcggtcagtg cgttacaaga tcgcgcctcg
     3481 ccgccccggc gacattgcct catgctatgc ggacccgcaa cgggcgattg agttgctggg
     3541 ctggagggca gagcgcgggc tcagtacaat gtgctccgac gcctggcgct ggcagcacac
     3601 gaatcccgac ggctatacgg gctgactttc atcactgggg gaatcgatga ggtaccgagg
     3661 tcgcgcgggc agatcgatga cgatctcttt ggcgagacag gggggaatat cttccttcag
     3721 ccgcttcaag agcctgggaa atttcatggt ggaaggcacc tcacataccc agaggtgtgt
     3781 gccggattcc agttcttcga tgtgtcgctg gacgcgaaac cccggatgct ccatgaaata
     3841 cgcggataag aatcctccga tggcctggac cacccacggg tgtgcggtgg cataacggta
     3901 gctgattcga agctcaacga cttgtgctgg ttcttgctca tccatcgttc gttggaagta
     3961 tagcctgtct ggatagagaa tccctcagat tttcttgtgt ctgccttgcc cgtgcttgac
     4021 cagctccata tctgtgtgta gggtcctgcg aaattcggtt ttctcactcg tttcaccagg
     4081 cacaattaaa aggctacaga tgaacattgc cattatcggt ggggggccgg ctggactcat
     4141 ggctgcggag tcggcacgcg ccgcaggtgc tcaggtcgat ctttatgatg ccatggcatc
     4201 ggtcggtcgc aagtttctcc tggctggaaa aggtggcttg aacctcacac actcggaacc
     4261 ctctctgaag tttctctcgc gttacggaga acgacgagcg cagatcgcgc tcttgctctc
     4321 gtcgttcggg cctgatgctc ttcgagcctg gaccagcggt ctcggtatcg gaacatttgt
     4381 gggctcttcc gggcgcgtgt ttcccactga tctcaaggct gcgccgttgc tgcgcgcatg
     4441 gctccgccgg ttgcgccagg ccggtgtcag attccacatg cgtcatcggt ggtcgggctg
     4501 ggacgagcag ggagctctct atttttccac accggaggga gcccgctctg cccaggccga
     4561 tgcggtggtc cttgcccttg gtggaggtag ctggccgaaa ctcggttctg atgcttcatg
     4621 ggtgtccgta ttagctaaac gcggccttca cattgccccg ttgttgccgt cgaactgtgg
     4681 attcgacgtg ggttggagcg agcattttcg agccaagttc gccgggcagc cggttaagtc
     4741 ggtggcggtc gtaatacgga atgctgccgg tgctgaatcc tggcatccgg gggaattcgt
     4801 gatcacggag accggcgtgg aaggtggcgt gatctatacg gtatccgcct ttcttcgcaa
     4861 cgaaattctg gcgaagggca tggctacctt
//
LOCUS       DATIPF010000015         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_444035, whole genome shotgun sequence.
ACCESSION   DATIPF010000015 DATIPF010000000
VERSION     DATIPF010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444035"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VLL06_00395"
     CDS             complement(<1..954)
                     /locus_tag="VLL06_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HSS29454.1"
                     /translation="MIQQPQRSSFDLLRRLWTLITPFRLQLACLGAVVLLSIPLSLLA
                     PVPLTLAVDSIIGSRPLPALLQAWVPLSIQQSTTALFLLMFLTFIGIALCTHLQGMVL
                     LLLSSHTGERMILAFRSRLFEHLQHISSSYHDTHGTADSVYRLQHDAASIKQIPIDVL
                     IPFLRAVCLLIGLSTLLIIINWRFALVAMGMLPLLFWLTRRCGRQLRSTWYALRSTES
                     ATIACAQEVLSASRVVKAFGREDHEHRRFADHAMDWVKKHNSLASVGSGFDFMFGMAV
                     ATGTATALVVGLHQVKSGWLTIGDFLLLMAYMSQLAGPLDTA"
     gene            complement(1676..1975)
                     /locus_tag="VLL06_00400"
     CDS             complement(1676..1975)
                     /locus_tag="VLL06_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29455.1"
                     /translation="MLAGVIVDDLATKAADGKSPASDIGNQLTLLVKREVTDANLTFD
                     SNSNLGLRLLRAFFLVSSPSIRRWVQNTRRSILQSLSSSRTTPFVPDLNPARSSF"
     gene            complement(2039..>2243)
                     /locus_tag="VLL06_00405"
     CDS             complement(2039..>2243)
                     /locus_tag="VLL06_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29456.1"
                     /translation="CFRLDTSYDQLVVAPIMTNLDAPGGGTDYAKTPKGNLDISGKYF
                     IWTSNLGGNRLDAFLVKVPSQLL"
BASE COUNT          577 a          550 c          659 g          457 t
ORIGIN      
        1 ggccgtatcc aatggcccgg ccagttgact catgtaggcc atgagtagga gaaagtctcc
       61 gatcgtgagc catccggact tgacctggtg gaggcccacg accaaggccg tagccgtgcc
      121 tgtggcgacc gccataccaa acataaaatc gaagccgctt cccactgaag cgagagagtt
      181 gtgcttcttg acccagtcca tggcgtgatc ggcgaagcgt cgatgttcat ggtcttcgcg
      241 tccgaaagcc ttaaccactc gagacgcgct gagaacttct tgggcgcagg ctatggtagc
      301 gctctccgtc gatcggaggg cataccaggt gcttctcagc tgcctgccgc accgacgggt
      361 cagccaaaac aggaggggca acatacccat cgccacaagt gcgaatcgcc aattgatgat
      421 gatcagtaac gtggagagtc cgatcaacaa gcatactgca cgaaggaacg gaatcagcac
      481 atcgatgggg atctgcttga tggaggcggc gtcatgttgg aggcgataga cggaatctgc
      541 cgttccatgc gtatcatgat aagacgaaga gatatgttgc agatgctcga acagtcggct
      601 gcgaaaggcg agaatcatcc gctcacccgt gtgggaagac aacaacaaca ggaccatccc
      661 ctgaagatgg gtgcacagcg caataccgat aaaggtgagg aacatgagta ggaaaagtgc
      721 ggtcgtcgat tgttgtattg agaggggcac ccacgcctga agtaaggcag ggagaggccg
      781 ggaaccgatg atactatcca cggcgagtgt gaggggcact ggtgctaaca aactgagagg
      841 aatggaaagg agcacgaccg cgcccagaca agccagctgt agacggaagg gagtgatcaa
      901 cgtccagagc cgacggagta gatcaaatga cgagcgttgg ggttgctgta tcacgtataa
      961 cacctccacg atctttcagc tctgtgactg accgatccgt cgataagaat cccgcgattt
     1021 ttttgagtaa gagatggggt gggacactgg atgacccact aactcatcat tctcttcggt
     1081 cattgtggta actacgcaag gtgtgtgccg ttgcagggac agagaggata tgaataaaaa
     1141 ccggaacgta aggtatggga cggggggaac atttatgcga gcgaggtgtc gaaaggctgt
     1201 aggagattgg atagattcag agccgaacgt cctatttctc agaagatagg atgtaggctc
     1261 cggcctacgc tgggtacagt caagtcgagg ttaacaaatg gtaaaaagag tacggttatc
     1321 aacaatcaga cgtggcggag gggatcggat cgcataccga cttgggctct cacggtaacg
     1381 gttgactagc tcataggctt gccggctctc gccaacggcc tgtatacgtt tagtctctca
     1441 ctagccgatg gaaagaagag gaccagggaa atgcctcgca gtgatctaaa atgagctagc
     1501 ctggacgagg ggcttcttcg tagtagacga atccaagcga tgacagatgg gagagggttg
     1561 aacacgtcac ctagatgcgt ggcatagtcc catggtccct tcatgaagtg atacacggcc
     1621 agaccagact tcccgcaata gaagcagcgt cctcttcatg agcatctcat gcattttaga
     1681 atgacgaccg tgccgggttt aaatctggaa cgaacggcgt cgttcgtgac gacgacaggg
     1741 actggaggat agaccggcgt gtattttgta cccaccttcg tatagaggga ctggacacaa
     1801 ggaaaaacgc gcgcaacaac cgcagtccaa gattggagtt gctgtcaaat gtcaggttcg
     1861 catccgtgac ttctcgcttg accagcagcg tgagctggtt gccgatgtca gaagccggac
     1921 tcttcccatc tgctgccttt gtcgccaggt catcaacgat gaccccagcg agcaccagcc
     1981 aatctctcgc gaaagcactt tcagagtgag tgacactatg agagctgtga ctctgttgtc
     2041 aaagtaattg ggagggcacc ttgaccaaga aggcatcgag ccgattcccg ccgaggttgc
     2101 tggtccaaat aaaatacttg ccgctgatat ccaggttccc cttcggagtc ttggcataat
     2161 cggttccccc gccgggcgca tccaggttcg tcatgatcgg ggccacgacc agttggtcat
     2221 agctcgtgtc gagtcgaaag cag
//
LOCUS       DATIPF010000016         3366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1239971, whole genome shotgun sequence.
ACCESSION   DATIPF010000016 DATIPF010000000
VERSION     DATIPF010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239971"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VLL06_00410"
     CDS             complement(<1..388)
                     /locus_tag="VLL06_00410"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSS29457.1"
                     /translation="MRTRGSTQSGACLFALCLFVTACGDRSTDSTSSPAKTNPQSSAP
                     RISTIQAPPSVRDRVRIDTVAMHVVPEVVTAPGEVALDLKQVAQITSRIEGQVEKIHV
                     QLGDRVKPGQPLAAIVSLQFDQLIEEY"
     gene            complement(544..1032)
                     /locus_tag="VLL06_00415"
     CDS             complement(544..1032)
                     /locus_tag="VLL06_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29458.1"
                     /translation="MARGRLLTVFWLTTWMVVIPFFHIHPEADHHHGDASHTHGGTIH
                     TVFSPDLECEFTESVHDSTCPEAAHQHPQTSAHFGHAVNHVELDFSLLTVPVDRPQPK
                     PGIIIAEFPSDGSTTTDRAIVVAALQPDTPPNIVFLSTALPLRAPPVSSLSSIFRVLI
                     IT"
     gene            complement(1222..1869)
                     /locus_tag="VLL06_00420"
     CDS             complement(1222..1869)
                     /locus_tag="VLL06_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29459.1"
                     /translation="MTRLLLLLLVCLWGPAYVDASTGEDITWNYDEDRSGQLPPKFMI
                     GTFSDGRPAGDWKVITMPKALSPPNVFAQSRAKGAGYNVVLIDNAQAADLDLSVSFLP
                     ISGKTDMGGGLIWRATDDRNYYLTRANPLEQNIRVYHVVKGIRKMIGDYNEIIDIKKW
                     HRLHVVNQGCHIQIFYDEKPIFDLCDETFKEGRIGLWTKSDAVTYFDDLRLQFLP"
     gene            complement(2065..2988)
                     /locus_tag="VLL06_00425"
     CDS             complement(2065..2988)
                     /locus_tag="VLL06_00425"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HSS29460.1"
                     /translation="MNKDHIAGSVLAFILVLSSPVQAHEAHAESSPTLDIKAIEQAIG
                     KPGEMKDDVYKISLPRKDLSVSVKGVQLKPGFALGSWIAFRQAGKKAIVDGDLVLTEE
                     EVGPVFGKLRKEGIEVSALHNHLIGETPRVMFLHIEGQGDAREVAMHLKEALNVTATP
                     IGPAPKVVGTAMTNDSEEADFDAELIQKELGHKGNIKNGVLQISVPRNESIKMGGVPL
                     PPSMGMATSFNFQSSGEHKVAATGDFVMVRDDVDRVTKALTEHGILITALHNHLVHGS
                     PDLYFMHFWAADSPEKVSKGLRAGLDVMKRG"
BASE COUNT          791 a          921 c          869 g          785 t
ORIGIN      
        1 ggtactcttc aatcaactga tcgaactgca aactcacgat ggccgcgagc ggctgaccgg
       61 gcttgacccg atcaccgagc tgcacgtgga tcttctcaac ttgtccttca atccgggacg
      121 taatttgggc gacctgcttt aaatcgaggg cgacttcgcc tggggccgtc accacttctg
      181 gaacgacgtg catagcgacc gtgtcgatcc gtactcgatc ccgcaccgac ggcggggcct
      241 gaatggtgct gatgcgagga gcgcttgact gtggattggt tttcgccgga gatgatgtcg
      301 agtcggtgct gcgatctccg caggccgtga cgaacaggca cagggcgaac aggcacgcgc
      361 cgctctgagt gctccctctg gttctcatta atcaggctcc tatctggatc tcttcgcaga
      421 cgatccggtc aaaccaccca tggtaagccg gacggctcaa ggtggacgcc atcgcgatct
      481 ccggaggtca gaaaatggga acggcagcgc cgctcaccca atatctgaca gcgagaggct
      541 gcgttacgtg atgatcaaga cacgaaaaat cgatgagagg gatgaaaccg gaggagcacg
      601 cagagggagg gcagtggaaa gaaacacaat gttgggcgga gtgtcgggtt gcaacgcagc
      661 cacgacgata gctcggtcag tcgtggtact tccgtcggat gggaattcgg caatgataat
      721 ccctggcttc ggctgtggac gatcgaccgg cacagtcagg agtgaaaaat cgagttcaac
      781 atgattgacc gcatgaccga aatgcgcact ggtttgggga tgctgatgcg cggcttcagg
      841 acaggtcgaa tcgtggacac tttctgtgaa ctcgcactca aggtctggcg agaacaccgt
      901 atggattgtt ccaccatgag tatggctcgc atccccatga tgatgatctg cttcgggatg
      961 gatatgaaaa aacgggatca cgaccatcca cgtcgtgagc caaaacactg tcagtaaacg
     1021 gcctcgcgcc acgcgtaatt ccttcccgca cccaggcatg gtaaccttcc gccaatttca
     1081 ggctacacca cttcgtatgc gggagcaagt agcaggacat ttcttgttgt cgattcagac
     1141 aatccagaga gagaacgagt gctcgactgc catatgtcga gacaagaaaa agaaagtccc
     1201 atacctccta cgggacaaat ctcagggaag aaactgaagc cgtagatcat cgaaataggt
     1261 gaccgcatcg gacttcgtcc acagcccaat gcggccctcc ttgaaagttt catcacagag
     1321 atcaaagatc ggcttctcat catagaagat ctggatgtgg cagccttggt tgaccacatg
     1381 cagccgatgc cattttttga tgtcgatgat ttcgttataa tcccctatca tctttcggat
     1441 gcccttgacg acatggtaga cgcggatgtt ctgctccagc ggattggcgc gggtgagata
     1501 gtagttccga tcatcggtgg cccgccatat gagcccgccc cccatgtcgg ttttgcccga
     1561 gatggggaga aaggacactg atagatcaag gtcggccgcc tgagcattgt caatcagcac
     1621 aacattgtag ccagcacctt tcgcccgaga ctgcgcgaat acgttcgggg ggctcaaggc
     1681 cttcggcatg gtaatgacct tccagtcgcc ggccggccga ccatcagaga aggtgccaat
     1741 cataaacttc ggcggtagct gtccagaacg atcttcatcg taattccacg tgatgtcttc
     1801 tcccgttgaa gcgtcaacgt atgcgggccc ccagaggcag acaagcagga gtagcaatag
     1861 acgcgtcacg aaggctcctt ctcccctttc tttttcattg cctccaagcc tgcgcgaaga
     1921 cctttagaca cccctctgca ctatcattcc cccagaaatg catgaagaag agggtgggcg
     1981 agctgtgata attgggaggg accaaatcac tgactccctc gactcataga gtcctcgtat
     2041 tgagatcgtc tccttcagat gcatttatcc ccgcttcatg acatcgaggc cagcgcgcag
     2101 tcctttcgag actttctcag ggctatcagc ggcccaaaaa tgcatgaaat agaggtccgg
     2161 ggacccgtgg acaaggtggt tatgtaatgc ggtaatcaag atgccgtgtt cggtcaatgc
     2221 cttggtaacg cgatcgacat catctcgcac catcacaaaa tctccggtag cggcaacctt
     2281 gtgttctcca ctggactgga aattgaacga cgtcgccatg cccatgctag gcggtaacgg
     2341 gactccaccc attttaatag attcattcct gggcactgag atttgcaaca cgccattttt
     2401 gatgttcccc ttatggccaa gttccttttg aatgagttcc gcatcaaaat cggcttcttc
     2461 actgtcattg gtcatcgcag ttccaaccac cttgggtgcg ggtcctatcg gtgtggccgt
     2521 aacgttcaag gcctctttga gatgcatcgc cacttcgcgt gcatcgcctt ggccctcgat
     2581 gtggagaaac atcacacggg gtgtttcgcc gatcaaatga ttgtgcaggg cgctgacttc
     2641 aatgccttct tttcgcagtt tgccaaatac cggcccgacc tcttcctctg ttaataccaa
     2701 atctccatct actatcgctt tctttccagc ttgcctaaag gcgatccaac ttcccagcgc
     2761 aaaacctggt ttcagctgga ctcccttcac ggagaccgac aagtctttcc gtggcagcga
     2821 tatcttatag acatcgtcct tcatctctcc gggttttcca atcgcttgtt caatggcttt
     2881 gatgtcgagg gtgggcgatg actcggcgtg tgcttcgtgt gcttgaacag gcgaactcaa
     2941 aaccagtatg aacgccaaaa cacttccagc aatgtggtcc ttattcatgg cggaccctcc
     3001 ttccagctct ctaggtgaaa gcaaatgcta tcgccattat agggtgtggt aactgatgat
     3061 gcccctagtt tgagttccga cttccttagc ccaattgaca ccaggcgacg aaggccttga
     3121 aatcacggta ggcattcctt gtgataattt gtgacagaag gtcaaattca cagacgagtc
     3181 cgcagagtcc gcgcaaccga ctgatttgac gaaagaacgg agaagccctt gacggcaaac
     3241 ccttctaaaa taatggccct taaaggcttc tgtctaagac actgaaagtg ttcgcgcctc
     3301 cctcaggcat tcacctaatc ataggttctc ctgatcctgt tcgtgcctac cgccggcacc
     3361 tcgaat
//
LOCUS       DATIPF010000017         4754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_532640, whole genome shotgun sequence.
ACCESSION   DATIPF010000017 DATIPF010000000
VERSION     DATIPF010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532640"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VLL06_00430"
     CDS             <1..1004
                     /locus_tag="VLL06_00430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS29461.1"
                     /translation="GRALLDLLHQFPSIEIVGIADRDPAAPGLQRARELRIPVTTNVP
                     DLISNHSASLIMDVTGDVEMKAYLHAHRPATADILSGTSSRLLWELVQHESKLQAQLL
                     QSEKLAGVGSFAAGIAHDINNPLQLILGLAENLTEERDLIAVHEQARDIIEAVKRTSA
                     ICRDLTRYSRRASPHEDMLVSVNGKLDEALKIARYASSFHDIEMVKHYQPGAAVNGNP
                     DELLHVLVNLITNAVHAMEQAGGTLTLETSLLDGEVGVRISDTGCGIAPDKIPEIFEP
                     FFTTKAPGKGTGLGLYNVKTIVNKMHGVIAVDSQVEKGTTFTLTFPNSGLSGEGDAS"
     gene            1001..3517
                     /locus_tag="VLL06_00435"
     CDS             1001..3517
                     /locus_tag="VLL06_00435"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS29462.1"
                     /translation="MNPAPPPSRSDTRWGLQLKLILSMLLVGVVPLIVGLGLAFWQGS
                     REIQVVSGESFKALAEETARKVDLLMSDEVGRSSRIAADPSIILELEHRRDALLDVTD
                     NERRAMETLKKGWEAKDPASVQTITGSKMAGLIQEYLSGAKSEADQLIPQVVQSATKM
                     LYVTDVEGNLVAASTTLAPFANKDTAWWKGASHRGVGQLFIEDVHFDERAQTYTISIS
                     LPIMDRIRYGVVGILHRVIDAKELISPSISPIRFGKTGHVMLIDHRGIVVSCPILPTG
                     VQLSDAQLIPLVTPLQPGWVSAPSDGHGGQSTSIIGFAPMPETSRATNSDQGKGSWHT
                     FVWQASDELFAPIRHFLTWMAIFSLIAVGLMTTLGYLAANRIVKPARRLQAAAQSIGR
                     GELREPIDIQTGDEIQDLAEEFNRMNRQLEAAFAGLTDQVAIKTQQVESLIHSTDEIL
                     DAVPTPIIMVNQDEQVQYINRVGRDSFQPQSDSTHSIPLFDILPADAALREHLRKEFR
                     RTRGGDNLATASEVEDVAAVNRARDPLAPMKGTGEVGNRQEIQIGPRRYHYQWFHMAG
                     RHQGEDRVGLVFRDTTDDSRLQDQLIQAEKSVSLGTLTAGIGHELNNPLFGILGLGEA
                     IGEETNPELVKSYARDIVRHGRRMAAIIRDFTGVTARETSDEWQPVHLERELDQAVAT
                     LTDSMNASGLAIQRTYAGDTCVLALPDQLRQAFTNLLMNAVQAIKGRGILRLSTSLTE
                     TTVVTTIADSGPGIPKEYLSKIFDPFFTTKGQGEGSGLGLTVARRIIRKFGGDIRIES
                     QEASGTSCIVTLPIVPPLPPPQEASCTESTSRSTPQPSPS"
     gene            3469..4062
                     /locus_tag="VLL06_00440"
     CDS             3469..4062
                     /locus_tag="VLL06_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HSS29463.1"
                     /translation="MHRVHVSLYAAALTILMSGLWWGLDTVAAKERQGSPGIPPEKVA
                     AYIYDVIKADRTLYTTEIVNRLQAKGVTTASEHWEQENALMLPAQFLQHAGKLAAENG
                     SGIRYRLIGLWPIYRRNAPSSDLERNALESLRKNPDVPVTGIVTSGRKQYFQAIYPDL
                     AVSPACIDCHNGHLLSPKRDFKLNDVMGGIAITIPLE"
     gene            complement(4324..>4754)
                     /locus_tag="VLL06_00445"
     CDS             complement(4324..>4754)
                     /locus_tag="VLL06_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSS29464.1"
                     /translation="VQGLVAGLAYGLLGVPFPVVLTAVTVLLAPLPFGGTALVWGPVV
                     FYFFLVGPFWKALVMLGWGIGVVTMIDQFLRPWLIGQGAQIPVFFLVFGVLGGLALYG
                     LIGLFVGPVLVSLLMTIIQIYREEYHNNETGTPTSPPTPS"
BASE COUNT         1125 a         1427 c         1333 g          869 t
ORIGIN      
        1 gcggccgtgc gctgttggat ctgctgcacc aattcccgtc gattgagatc gtcggcattg
       61 cagaccgtga ccctgccgcc ccaggattgc aacgcgcacg ggaacttcgg attcccgtca
      121 ccacgaatgt tccggacctg atcagtaatc atagtgcgag tttgatcatg gatgtcacgg
      181 gcgacgtgga aatgaaggcg tatctgcatg cccacagacc cgcgactgcc gacatcctca
      241 gtggaacatc atcccggctg ctgtgggagc tggtccagca tgaatccaag ctccaagccc
      301 aactcctcca gtccgaaaag cttgcgggag ttggctcatt tgccgcggga attgcgcatg
      361 atatcaacaa ccccctgcaa cttattttag gcctggcgga aaacctcacg gaggaacgcg
      421 atctgatcgc cgtccacgag caagcccggg acattatcga agccgtcaaa cggacaagcg
      481 ccatctgccg ggacctcacg cgctactcgc ggcgcgcatc ccctcacgag gatatgctgg
      541 tgtcggtcaa cggaaagctg gacgaggcgc tgaaaatcgc acgctacgcg tcgagttttc
      601 acgatattga aatggtcaaa cattaccagc ccggcgccgc ggtgaatggt aatccggatg
      661 agctattgca tgtcctcgtc aatctcatta ccaacgcggt ccatgccatg gaacaggccg
      721 gcggcacatt gacactcgaa accagcctcc ttgatggaga ggtcggcgtc cgaatctccg
      781 atacgggatg cggcatcgca ccggacaaga tcccagaaat cttcgagcca tttttcacga
      841 ccaaggctcc cggcaaaggc acggggctgg ggctctataa tgtcaaaacg atcgtcaaca
      901 aaatgcacgg cgtcattgcc gtcgacagtc aagtcgagaa gggaacgacc tttaccttga
      961 ccttccccaa cagcgggctg tccggagaag gagatgcgtc atgaatcccg cacctccccc
     1021 gtcccgctcc gacacaaggt ggggcctcca actcaaactg attctgtcca tgttgctcgt
     1081 gggagtggtt ccgctcatcg tggggctggg tctggccttt tggcaaggct ctcgcgaaat
     1141 ccaggtcgtc agcggcgaaa gcttcaaggc cttggccgag gagacagcac gaaaagttga
     1201 tctcttgatg tcggacgaag tcggccggtc atcacggatt gccgcagacc cttcgatcat
     1261 cctcgaactt gagcaccggc gcgatgccct gctggacgtg acggacaatg agcgacgggc
     1321 gatggagacg ctcaagaaag gctgggaggc caaagacccc gcttccgtcc agaccatcac
     1381 aggaagtaaa atggccggcc tcattcagga atatctctcc ggggccaaga gcgaagccga
     1441 tcaactgatc ccgcaagtgg tccagtctgc caccaagatg ctgtacgtca ccgacgttga
     1501 gggaaatttg gttgccgcat ccacgacgct ggcgccgttt gctaataaag acacagcctg
     1561 gtggaaaggt gcttcccatc gcggagtcgg ccagctcttc attgaagatg tgcacttcga
     1621 cgaacgtgcg caaacctata ccatcagtat ttcgcttccc attatggatc gcatccgata
     1681 tggcgtcgtc ggcatactcc atcgggtgat tgacgccaaa gagttgatct cgccttcgat
     1741 ctcgccgatt cgcttcggca agaccggaca tgtgatgctg attgaccatc gagggatcgt
     1801 cgtcagctgc cccatcctgc cgaccggcgt ccaactctcc gatgcacagc tcattcccct
     1861 cgtcacaccg cttcagccgg gctgggtcag tgcgccgagc gatggccacg gaggacaatc
     1921 gacctcgatt atcggattcg ccccgatgcc ggagaccagc cgagccacca atagtgacca
     1981 gggaaagggc tcctggcaca ccttcgtctg gcaagcgtcc gacgagctct ttgctccgat
     2041 tcgtcacttc ctcacctgga tggccatatt cagtctcatt gccgtcggat tgatgacgac
     2101 gctcggctac cttgcggcca atcgcatcgt gaagccggcg cgccgattac aggccgcggc
     2161 acaatcgatc ggacgcggcg agcttcgaga gcccatcgac attcagacag gcgatgaaat
     2221 ccaggatctg gcggaagagt tcaatcgaat gaatcgccaa ttggaagcgg cctttgccgg
     2281 cctgaccgat caagtggcca tcaaaacgca acaagtggaa tccttgattc actcgacgga
     2341 cgagatcctg gatgcggtcc cgaccccgat catcatggtc aatcaagacg aacaggtgca
     2401 atacatcaac cgggtcgggc gcgactcctt tcaacctcaa tctgattcga cccactcaat
     2461 cccattgttc gacatactcc ccgccgatgc cgcgctacga gagcacctgc gaaaagaatt
     2521 ccgacgtaca aggggcggag acaatctcgc aacagcatcg gaagtagagg atgtggcggc
     2581 cgtcaaccgc gcccgggacc cattagcccc catgaaggga acgggggaag tcggcaaccg
     2641 gcaggagatt cagattggtc cccggcggta tcactatcag tggttccata tggcggggag
     2701 acatcagggg gaggatcggg tcggattggt cttcagggac acgacagacg acagccgact
     2761 ccaagaccag ctcatacagg cagagaaatc agtcagcctt gggaccctca cggccgggat
     2821 tggacatgag ttaaacaatc cactcttcgg aattctcgga ttgggagaag ccatcggaga
     2881 agagaccaac cccgaactcg tcaaatccta tgcccgcgac atcgtccggc atggccgcag
     2941 aatggccgcg attattcgag acttcactgg tgtcacggcc cgcgaaacgt cggatgaatg
     3001 gcaaccggtt catcttgagc gagaactcga tcaggccgtc gcgacactca cagacagcat
     3061 gaatgcctcg gggcttgcaa tccagagaac gtacgcggga gacacctgtg tactggccct
     3121 gcctgaccaa ctcagacagg cattcacaaa cctcttgatg aatgccgttc aggcgatcaa
     3181 gggccggggc atcctgcgtc tctccacgtc cctgaccgag acaacagtcg tgactacgat
     3241 tgcggattca gggcccggta ttccaaaaga gtatctgtcg aaaatctttg atccattctt
     3301 taccaccaag ggacagggcg aaggatccgg actcggcctg accgtcgcgc gccgcatcat
     3361 cagaaaattc ggaggagaca tcagaatcga gagccaggag gcgagcggca cctcgtgtat
     3421 cgtcaccttg cccatcgtcc ctcctctacc gcccccacag gaggcttcat gcaccgagtc
     3481 cacgtctcgc tctacgccgc agccctcacc atcctaatgt ccggactgtg gtgggggctc
     3541 gataccgtag ccgcaaagga gcgccaggga tcgcccggca ttccgccgga gaaagtggca
     3601 gcttatatct atgatgtcat caaggccgat cgcacactgt atacaacaga aatcgtcaac
     3661 cgacttcagg caaagggcgt cacgacagcg tccgaacatt gggagcagga gaacgccctc
     3721 atgttgccgg cacagttcct ccagcatgcg ggcaaactgg cagccgagaa cggaagcggg
     3781 atccggtatc ggctaatcgg tctctggccc atctaccggc gcaacgctcc gtcaagcgat
     3841 ctggagcgca acgcgctgga gtccctccgg aagaatcccg acgtgccggt caccggaatc
     3901 gtgaccagtg ggcgcaaaca atactttcag gcgatctatc cagatctcgc cgtctcaccg
     3961 gcctgtatcg actgccacaa cggtcatctg ctcagcccga aacgggactt caaactcaac
     4021 gatgtaatgg gcggaatcgc catcaccatt ccattggaat agaaacgtcc aaatacattc
     4081 tcgcaggatg ctcaaaaagg ctgtcagcaa ggccgcaggc aaacaagaac cggaggcgta
     4141 ccctctggag cgcacggtgc gacgaataag gagcaccacg tttgtgcgcg ccgccgagtg
     4201 gtgaggcggc tgggtccccg ttgaggattc ttgtgagctg agaacgaagc ccggggagag
     4261 gcgcgtctcg gcgcgccggg gttgggcggg tgagaacagc gactttttca gcatcctgcg
     4321 gggctaggag ggagtgggag gactcgtggg cgtacctgtt tcattgttgt gatattcctc
     4381 ccggtagatc tggatgatgg tcatcaacag actcaccaga accggaccga cgaagagccc
     4441 gatgagaccg tagagtgcca gtccgcccag gacaccgaac acgagaaaga atacggggat
     4501 ctgggccccc tgtccaatca accagggccg aagaaactga tcgatcatcg tcaccacgcc
     4561 gatgccccag cccaacatga ccagcgcttt ccagaatgga ccgaccaaga agaaatagaa
     4621 cacgaccgga ccccacacca aggcagtccc gccgaaggga agcggcgcaa ggagtaccgt
     4681 cactgccgtc aggacgaccg ggaaaggcac tccgaggagt ccataggcca gacctgccac
     4741 cagaccttgc acga
//
LOCUS       DATIPF010000018         8570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_178862, whole genome shotgun sequence.
ACCESSION   DATIPF010000018 DATIPF010000000
VERSION     DATIPF010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8570
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178862"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VLL06_00450"
     CDS             complement(<1..1029)
                     /locus_tag="VLL06_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HSS29465.1"
                     /translation="MKRLPTIGSAVIDRLHQLGVRHIFGIPGDYVLSLYQLIEASPIQ
                     HVGTTREDSAGFAADAYARINGIGAACVTYCVGGLNTVNAIACAYAERSPVVLLTGSP
                     GLSERIRTPYMHHMVRDFSTQREVFEKITVAAISLDDPSTAEREMDRAFAALLRYRRP
                     IYIEIPRDLVHTPLASPLRPLCLDDQPSDIAALDEAIAEVHAMLASAKRPAILAGAEI
                     GRFGLQDDLTRLVEQLNVPIASTLLGKSIIREDHPLYVGVYGGLIARDEVQQFINESD
                     CLLILGSILSDVEDLDAHSPLLSEGRTIHATADRVAIKHHRYDAIRFQDFVKALVTNP
                     LPTFPTRPL"
     gene            1171..2364
                     /locus_tag="VLL06_00455"
     CDS             1171..2364
                     /locus_tag="VLL06_00455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HSS29466.1"
                     /translation="MALHDDSPRGRIRLLHTRAVEAVGHLWVYAGYVEEASSELVSGD
                     VVDVMAPSGRFYARGLYNPESKIRVRILTFADEPITEGFWRERLAQAVRLRQRIVTGT
                     NAYRLIYGEADRLPGLIVDRYDDILVMQTLSSGMDRRKYVLADLLCQESGAARVYLRN
                     DAKSRMLEGLPVHKGFLRGGGATMVDIYEGSAQFLVDIERGQKTGWFCDQRENRIATA
                     GLAAGGEVLEVFAHTGAFGIHAALAGAKSVEGLDVSEEAVALARNHAVLNKVDSCCVY
                     RSAEAFEEMRKLERAGRKYDLVLLDPPAFARSKQAVPRALAGYKDVNLLGMRLTKPEG
                     FLVTSSCSHHVTEQELWTGIRLAARDAKRQVRLLEQRGQASDHPILATMPETRYLKCF
                     ILQVL"
     gene            complement(2637..3098)
                     /locus_tag="VLL06_00460"
     CDS             complement(2637..3098)
                     /locus_tag="VLL06_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSS29467.1"
                     /translation="MALGQQIQAWRISRGHSIDDLATESHLLPRVLEDIEAEIIDPSA
                     SLLEVLAAAMKIPPAWLFSHPTSFRTLFDDPEEDTSSPPTGPDPVTERILSGSRMDRS
                     LYVLLTAIIQSGEPKLLRAAEVNLRSLVKQAKQATVPWQNRPSGHFEPPSD"
     gene            3272..3613
                     /locus_tag="VLL06_00465"
     CDS             3272..3613
                     /locus_tag="VLL06_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29468.1"
                     /translation="MLGTDIRGIIAEEEEVQRRKDALKSLLTMRSKQLRESLEQRIKR
                     ARICGDWIQLSQEECAMLHKQEKLHLKSQFDKLQHEQDRTRGKLTALKRAKARAQRIR
                     AAEAASGRKRR"
     gene            3737..4450
                     /locus_tag="VLL06_00470"
     CDS             3737..4450
                     /locus_tag="VLL06_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HSS29469.1"
                     /translation="MVEGAHAVRDLLTRYPEHILTIVTAHGYLPKEGHEDRLVRRSAS
                     LMRQYTCSDLQFSKLSDLEAPQGILAVVRQPTWDEGEVLRQSTVLGIFGEQLQDPANV
                     GTIIRTAAALNASALWLTPESADVYQPKVVRATSGMLLALPIFIAGDVSGLIRQGCQI
                     FAAEVGGLGTVPIDEIHQVPPKLILAVGNESRGLSAHLRKQATARFTIPLSRDVESLN
                     VAAVVAIATFYLSRLPREK"
     gene            complement(4590..5600)
                     /locus_tag="VLL06_00475"
     CDS             complement(4590..5600)
                     /locus_tag="VLL06_00475"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF007989.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HSS29470.1"
                     /translation="MNASRYHGVDRHVGNTPLIRLRRLSELTGSEILGKAEFMNPGGS
                     VKDRAALGIIEDAEARGLLRPGDTIVEGTAGNTGIGLTVIGHARGYHSVIIIPENQSQ
                     EKIDLLIMLGAEVVTVPEKPYSNPGNYNHVARRMAEEKGWFWANQFDNTANRSAHYRT
                     TGPEIWEQTAGEVTGFVSAVGTGGTLAGTTLYLKERNPRTTVGCADPYGAAMWSWFTN
                     GNTDTKDGDSFAEGIGQSRVTKNLEGLAVDCAWRIPDQDALTILYQLLREEGLFLGLS
                     SGINIAGAVRLALERGPGQTIVTILCDSGAKYQSRLFNPEWLAANGLRTDLSVDTLLG
                     KP"
     gene            6663..7229
                     /locus_tag="VLL06_00480"
     CDS             6663..7229
                     /locus_tag="VLL06_00480"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HSS29471.1"
                     /translation="MRSLRALGALAILALLFLAEPIAAIADDSFDDAKQPRLKAWSNI
                     IPTAKRFVVLADFNNEAVLDRETGLVWEQSPSSLRDDIKSWKDARLTCANKAVGRRQG
                     WRLPSIIEFASLIDPSIPSPGLSLPAGHPFNAQTSVRYWSATSSAADEVPSNAWVMYV
                     SGGIVQVEPKTNFRNIWCVRGPMNTDAY"
     gene            complement(7733..7808)
                     /locus_tag="VLL06_00485"
     tRNA            complement(7733..7808)
                     /locus_tag="VLL06_00485"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7773..7775),aa:Phe,seq:gaa)
BASE COUNT         1913 a         2207 c         2496 g         1954 t
ORIGIN      
        1 cagcgggcgg gttggaaatg tagggagggg gttggtcacc agtgctttga caaagtcttg
       61 aaaccggatc gcatcgtagc gatggtgttt aatggctacc cgatcggccg tcgcgtgaat
      121 cgtccgaccc tctgataaca agggcgaatg cgcatcgaga tcttcgacat cggataagat
      181 ggaccccagg atcagcaggc agtcagattc attgatgaac tgctggacct cgtcacgtgc
      241 gatgaggccg ccgtacactc cgacatagag tgggtggtct tcacggatga tggatttgcc
      301 tagtaaggtc gaggcgatgg gcacgttgag ctgttcgacg aggcgggtca gatcgtcttg
      361 caggccgaat cgtccgatct ccgcaccggc taaaatagcc ggccgtttgg ccgaagctaa
      421 catggcatgg acttcggcaa tggcttcgtc cagggcggcg atatcgctag gttgatcgtc
      481 gagacataag ggtcgtaacg ggcttgccag cggcgtatgc acgagatcac gggggatttc
      541 gatgtaaatc gggcggcgat agcgtaataa cgccgcaaag gcccgatcca tttcacgttc
      601 agcggttgaa ggatcgtcca gcgagatggc ggcgactgta atcttctcga agacctctcg
      661 ttgtgtggaa aaatcacgca ccatgtggtg catgtagggc gtgcgaatac gctccgagag
      721 tcccggcgat ccggtgagca gtaccaccgg agatcgttcg gcataggcac aagcgattgc
      781 attgacggta ttgagcccgc cgacgcagta ggttacacag gccgcgccga tgccattgat
      841 acgggcatag gcatctgccg caaatcccgc actatcctcg cgcgtagtcc cgacatgttg
      901 aatcggtgag gcctcaatta attggtagag gctgaggaca tagtcgcctg gaattccaaa
      961 gatatgccgg actcccaact gatggagccg atcgatcacg gcggaaccga tggtcggtag
     1021 tcgtttcatc gttgggcttc ctggtttata aaacggaaag catgatgtgc gttcgcatag
     1081 tcgcagcaaa ccatagcgcg ggtcaggcgt caagcaatca gatgtgtccg ggggcttgac
     1141 gttcggttcg cgtcattgga taagtacgga atggctcttc atgatgacag tccgcgcggt
     1201 cgcatccgcc ttttgcacac acgtgcagtt gaggctgtgg ggcacctatg ggtctacgcc
     1261 ggttatgtcg aagaggccag cagtgagctg gtctcaggcg atgtggtcga tgtcatggcc
     1321 ccgagtggac gattctacgc cagaggtctt tataatcccg agtccaagat ccgcgtacgg
     1381 atcctgactt tcgccgatga acccattaca gaggggtttt ggcgggaacg actcgcgcag
     1441 gccgtcagac tgcgtcagcg gatcgtcacc ggtaccaatg cctatcgcct catctatgga
     1501 gaagcagatc ggttgccggg tttaatcgtg gatcgttatg acgacatatt ggtcatgcag
     1561 acgctgtcct ccggtatgga tcgccggaaa tatgtattag ccgatctcct gtgtcaggaa
     1621 tccggagcgg cgcgagtgta tttgcggaac gatgccaaga gtcgcatgtt ggaaggcttg
     1681 ccggtgcaca aaggatttct tcgaggaggc ggcgcgacga tggtcgatat ctacgaaggg
     1741 tccgcacaat ttctcgtcga tatcgaacgc gggcagaaaa ctgggtggtt ttgtgatcag
     1801 cgagagaatc gaatcgcgac ggcagggctc gcggcaggag gggaagttct cgaggtattt
     1861 gcgcataccg gagcgtttgg tattcatgcc gcgcttgcag gagccaagtc ggttgaagga
     1921 ttggatgtga gtgaggaggc cgtggcgctc gcgcgcaacc atgccgtgct taacaaggtt
     1981 gatagttgtt gtgtctatcg atccgcggag gcattcgaag agatgagaaa gttagaacga
     2041 gctgggcgca aatacgacct ggtgttgctc gacccccctg catttgctcg aagtaaacag
     2101 gccgtgccgc gagctctggc tggatacaaa gatgtcaatc tgcttgggat gcggctcacc
     2161 aagccggagg gattcctggt cacgagttcc tgctcccacc atgtgactga acaggaacta
     2221 tggacgggca ttcgccttgc tgcacgagat gcgaagcggc aggttcgcct gctcgaacag
     2281 cgcggacagg ccagcgacca tcccattctc gccaccatgc cggaaacccg ctacctaaaa
     2341 tgtttcatcc ttcaagtatt gtgagcatga agtcggtagg atgaaatcgt cgctcggatg
     2401 caaggcgctc aaaacggctg tccagccaag gccgcagcga gtgaaggccc gaggcgtacc
     2461 ctctggggcg cacggtgcga cgaataagga gcaccacgtc tgtgcgcgcc gccgagtggt
     2521 gaggcggccg ggtccccgtt gagggtctga acgatgcgag aacgctgcat gggactaggc
     2581 acgtctcggc gcgccagggt tgggcgggtc agacgagagg attattcagc atcctctcaa
     2641 tcgcttggtg gttcgaaatg gccagacggg cgattctgcc aggggaccgt cgcctgtttg
     2701 gcttgtttga cgagactacg caaattcact tcggcggcac ggagtaactt tggttctccg
     2761 ctttggataa tcgcggtcag taggacatag agcgatcgat ccatccgtga tccggacaga
     2821 atgcgttctg tgacaggatc ggggcctgtg ggaggggagc tcgtatcttc ctctggatcg
     2881 tcgaacaagg ttcgaaacga cgtcggatga ctaaagagcc aggcaggagg gatcttcatg
     2941 gcggcagcga gcacttcgag gagcgaggca gagggatcaa tgatttccgc ttcaatgtcc
     3001 tctaacacgc gaggcagaag atgcgattcg gttgccaagt catcgatcga atgaccacgc
     3061 gagattctcc aggcttgaat ctgttgtccg agcgccatac cgccatggta ctgaaattga
     3121 ggccttcgcc gcaacctttc tctgtcggat gatggatgat tcttctttga acacaatagg
     3181 ttatgcgtaa tttctcccag tggaagatga gcaatgtaat caggtataat gccaagtgtc
     3241 attcattctc gatccagtgt ggaggcatac catgttagga acagatattc gtggaattat
     3301 tgcagaagaa gaagaagtac agcggcgcaa ggacgccttg aaatcgctcc tgaccatgcg
     3361 ttccaagcag ttgcgtgaat cgttggaaca gcgtatcaaa cgtgctcgga tttgtggaga
     3421 ttggatccag ctttcccaag aagaatgtgc catgttgcac aagcaggaaa agctccatct
     3481 caaatcccag ttcgacaagc ttcagcacga acaggatcga acccgaggga agttgactgc
     3541 actcaaacga gcgaaggcgc gagcccaaag gattcgagct gccgaagctg cgtccgggcg
     3601 gaaacgccgc tgaataagtc cttttcactg taacattagc gtgactgcat cacttcccct
     3661 cctcacgcgc tcgcaggggg cgcacatacg tgaactcctc cttgatagac acgtacggac
     3721 acaggaaggc gccttcgtgg tcgaaggcgc ccacgcagtt cgagatctcc tgactcggta
     3781 tcccgagcac attctcacca ttgtcacagc ccatggctat ctgccaaaag aagggcatga
     3841 agatcgactg gttcgtcgat cagcctcctt gatgcggcag tacacctgtt ccgatctgca
     3901 gttctcgaaa ttgtctgatc tcgaggcacc gcaaggaatt ctcgctgtgg tgcgacaacc
     3961 gacatgggat gaaggagaag tcctgaggca gtcgacagtc ttggggattt ttggagaaca
     4021 gttacaagat cccgcaaatg tcgggaccat tattcgaacg gccgcagctc tcaatgcgag
     4081 cgctctgtgg ctcacgccgg agtcagccga tgtctaccaa ccgaaggtgg ttcgtgcaac
     4141 gagcgggatg ttgcttgccc tgccgatctt tatcgccgga gatgtgtccg gattaatccg
     4201 gcagggctgt cagatctttg cagcagaagt gggagggctc gggaccgttc cgatcgacga
     4261 gattcaccag gtgccaccga agcttattct tgctgtcggt aatgaaagtc gtggtttgtc
     4321 ggctcatctt cggaagcagg cgaccgctcg attcaccatc cccctcagcc gtgacgttga
     4381 gtccctcaat gttgctgctg ttgtggctat tgccacgttc tacctctcgc gacttcccag
     4441 agagaaatga cctagagctc attcgtacgt gcgaatggac cgggcttcct cagcgccagc
     4501 atagaatcgg ccgcgctgca agatccctgt tttttctgtc gagagcggat tgccgtttgc
     4561 ggcacacatc aggtctatta gccacgggat cagggcttgc cgagaagggt atcgacggac
     4621 aaatccgtcc tcagtccgtt ggccgcaagc cattccggat tgaagagcct cgattgatac
     4681 ttcgcaccgg agtcgcaaag gatcgtcacg atggtctggc ccggtccgcg ttcgagggcc
     4741 agccgcaccg cgccggcgat gttgatgccg gatgacaggc cgagaaaaag cccctcctcg
     4801 cgcaggagct ggtaaaggat ggtgagtgcg tcctggtcgg gaatgcgcca cgcgcagtcc
     4861 acggcgaggc cctcaaggtt tttggtcacg cgggactggc cgatgccttc tgcaaaggaa
     4921 tcgccgtcct tcgtgtccgt gttgccgttg gtgaaccacg accacatggc cgcaccatac
     4981 gggtcggcgc agccgacggt agttcgagga ttgcgctctt tgagatacag cgtggttccg
     5041 gctagcgtgc cgccggtgcc gacggcggaa acaaagccgg tcacctcccc agcggtctgc
     5101 tcccagattt ccgggccggt ggtgcggtag tgcgcggagc ggttggcggt gttgtcgaat
     5161 tgattggccc agaaccagcc cttttcctcc gccatccgcc gtgcgacatg gttgtagttt
     5221 cctggattgg aatacggctt ctccggcacg gtgacgacct ccgcgcccag catgataagg
     5281 agatcgattt tttcctgcga ctggttttca ggaatgatga tgacggaatg gtagccccta
     5341 gcatggccga tgaccgtgag gccgatgccg gtgttcccag ccgtgccctc gacgatggtg
     5401 tcgccgggcc gcaggagccc tctggcctcg gcgtcctcga tgatgccaag cgcagcgcgg
     5461 tccttcaccg atccgccggg attcatgaat tcagccttgc cgaggatttc gctgccggtt
     5521 agctccgaaa ggcggcgtag gcggatgagc ggggtgttgc cgacatggcg atccacccca
     5581 tgatagcgtg aagcgttcat ctagtaggtt tgcaggtcca cccctgcaat ccgggaaaag
     5641 ggctaagcgt tgttgccccg gccagccgga tggcatagac cgtggtggcg atccggatta
     5701 cattgtcagg gagcgtcagg atttcgacag agatggtttt cgaaagcatg catggatttt
     5761 acacagaaaa tggcttggaa aatcaaagga aatcttcgcc cctcgcttca cactggtggc
     5821 tgcttcgctg cggaatgtgg taacccaggg tggatctgaa acaacggtca caggtaaacc
     5881 tgcgattgga tttcccatct gtggagtaat gacatcacaa attaagggat tgggggtgga
     5941 aacaaggatt ggctttttat tgtgaagagc catcggactt tccgagggaa aggcaagcac
     6001 ggagacaaga tccccaccct atggaggagt ttgggttagc caaggcataa ccttcttcga
     6061 tcagttaacc ctgtgtttca aattcagtaa atatttcccg gtctatgtat gtgtgcgggg
     6121 tcagggctat atggttcact tctgcgaatt ttttggcacc ccaaaatgtc caaaatctgg
     6181 ccgctagtga tctattggtg gttgtggatc cgtcgctggc aatgtagcgg ataccagcgt
     6241 cctcttgctg tttcgatccg gcaaagatca caaacagcgt ttgcttcttt ttccgtggca
     6301 gggcaccacc atcattgctt aatggcacta cggcaacagt cccttcttat ctacgaagct
     6361 gctgaactgg tcgggctgtc ttcacagggg aatcctaggt ccgcatgtat cacatattat
     6421 tccgctgttc tccacacatc tttagtaggt gtacgagagc tgcatggccc tattacgcat
     6481 aagtctaaaa acgtgctagg tgtctcccta gtaggcatgg cagactgcct cgatcatgat
     6541 taatgagaac tggaaaacat gagtgactaa aaatgttccg gtccacagaa gctttgagtt
     6601 gtgggacagt ctcgcccccg gcgttgctag aagcatcccc accgctacga ggccatgaaa
     6661 aaatgagaag cctccgtgcc cttggcgctc ttgcaatctt ggctctcttg tttcttgctg
     6721 aacccatagc cgcgatcgcc gacgactcct ttgacgacgc gaaacagccc cgcctgaaag
     6781 cctggagcaa catcatcccc actgctaagc gattcgtcgt attagccgat ttcaacaatg
     6841 aggctgtttt ggaccgcgaa acggggctag tctgggaaca atcgccatcc tcattgcggg
     6901 atgacatcaa aagttggaag gatgctcgcc tcacctgcgc caataaagcc gtcgggaggc
     6961 gccaaggctg gcggctgccg tccatcatag aatttgccag cttgatcgat ccctcaatac
     7021 cctctccggg tctgtcccta ccggcgggcc accccttcaa tgcccagact tccgtccgct
     7081 attggtcggc gacatcaagt gcggcggatg aagttccgtc caatgcgtgg gtcatgtatg
     7141 tcagcggtgg cattgtgcaa gtcgaaccta agaccaactt ccgcaacatc tggtgtgtgc
     7201 gtggtcccat gaatacggac gcctattgag gttggagaag tgagtggtag gaggtgaggg
     7261 tgacgacagc gcattggata cgctctggtt gggattttgc gtcagcacca actcctcaaa
     7321 tagcttggcg tccaggaagc acaccatagc ggacgtgctt cgcatagatc tgaatcgtga
     7381 ttttctccca caccttgaaa cacatctgaa agtcttcagg attagagtca ggtagtgctg
     7441 gagttggttc gcggtgggag gcttcagagt cacctggttt atggattagc tgatgggcag
     7501 atatcttgag gggccagtgg cagtggggtc agtcctctca tgttcttccc attcaaattc
     7561 aatcggatta aggctcgatt gctgaacagg atcaacattt tgttcttctg ggttcttagg
     7621 aggcgatcta acattggcca gtagaatttt cagcgctctg accgaaagag ggcctggcct
     7681 aattttcgat tttgttgaaa cttctaaacc actgtatggg tgcggcgttt ggtggtgccg
     7741 agggacagaa ttgaactgtc gacaccagcc ttttcagggc tgtgctctac caactgagct
     7801 acctcggcac ggtcatggta agacgagcga tgcactgtta caggatcatc agaaaaaatc
     7861 aaggtgtctg atcatctctt ctcagaggga gttctcaaac caggctgtgg cggcgaggga
     7921 ggaagtaaac cctgcttaac cgatgccctc cgctttgaat tacacactgt ggcgttggtg
     7981 gcctagtgtg gcgatcattt ttctttcgcg gcaatataca gctatcgtta accagcgtgc
     8041 aagagtttag aaacaggggc atcgcactgg tggcgtaagg gccaattcat cgcgcgtgga
     8101 aaaactaaat acaggtctgg tcactgccgc gccaaaataa aaaggccagt ccccttgtcg
     8161 aggactggcc gtgctcgcta tgcgctttgt gaactgaagt agacctaagt ggacttcctt
     8221 ctccaagccc agatgccaag tccagctaat ccagcaccaa ggagcagtag ggatgacgat
     8281 tcgggaacag atccgcactt ttttggctgg ctcccactcg gtccacaacc attggtatca
     8341 ctagcgccag gaatattgcc attgtccggc tgagccagtg cgagattacc agaaagcccc
     8401 atagtaacca ccgcggcccc taatgccaga gaaccgatta gtccaagccg tttcttcatg
     8461 actggtccct ccttttgagt gccagaatta aaaaactcgt cacacacgat agcaatatgc
     8521 atcattagtg tatataaatg ctaccccctc tttagggtta tagaggggct
//
LOCUS       DATIPF010000019         3279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_886413, whole genome shotgun sequence.
ACCESSION   DATIPF010000019 DATIPF010000000
VERSION     DATIPF010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886413"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1508)
                     /locus_tag="VLL06_00490"
     CDS             complement(15..1508)
                     /locus_tag="VLL06_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HSS29472.1"
                     /translation="MKDPSLRQLQEISALLNSSLDHATIRKRAIEAATLLMNAEAGSL
                     LLLDEATGDLYFDVAHGEKGEAVRQARLRQGQGIAGHVARTGEPIIVNDVQHDTRFFR
                     HVDQTSGFVTRNMVCVPVTAHGHLLGVLQAINHKHGEQFGDDDLQNFVALGHQVGIAI
                     ENANLYEEIHLLFEGFISASVQAIESRDPSTSGHSQRVATLTCRLADAVTQTDSGLYA
                     GIRFNAEQLKELRYAAVLHDFGKVGVREHVLLKARKLYQLQQELVKSRFDFIKRTMEV
                     DTLRRKLEVYEAKKSTGKHALLVQLDRQLAQRMDEVDKMFSFILKCNQPSSQSPAQIA
                     KLKDIARLEYHSYDGPRPFLTEEEVRALSVLHGSLTQSERKEIERHVTHTFEFLSKIP
                     WSRSLKNVPSIAWTHHEKLDGSGYPRGLPSNQIPLQARMMTICDIYDALTASDRPYKA
                     AISTDTALGYLESQSKEGKLDAELVEVFTREKVYQVTQRHSTIRKIA"
     gene            complement(1951..2316)
                     /locus_tag="VLL06_00495"
     CDS             complement(1951..2316)
                     /locus_tag="VLL06_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29473.1"
                     /translation="MSVMGRIGIMGLGIVLAGYLSGAPVFAEDKPTKPPIEDGSKVII
                     TSPKDGDEVDDTFELKYELTKGTQATHVHVYVDGEYQKGFSGTLKGLSKGDRKITVAG
                     ATKDHDLVVATHTIIIEVE"
     gene            complement(2403..2543)
                     /locus_tag="VLL06_00500"
     CDS             complement(2403..2543)
                     /locus_tag="VLL06_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29474.1"
                     /translation="MAVSRRVPVDLALTMLDAGCAHDVAHDMYQSATHTEQAIYPRRF
                     VT"
     gene            complement(2646..3011)
                     /locus_tag="VLL06_00505"
     CDS             complement(2646..3011)
                     /locus_tag="VLL06_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29475.1"
                     /translation="MHKVGRIGTIGLGIALACYLSGAPVFAGEQPPRPAAVDGSKITI
                     TSPKDGDKVNDSFELKYELIKGSQVTHVHVYVDDEYQRGFSGVLKGLSKGTHEITVKA
                     AMKNHSLAPPMDTITVEVE"
BASE COUNT          691 a          924 c          882 g          782 t
ORIGIN      
        1 agacgagggt gcagtcaggc gatcttgcgg atcgtcgaat ggcgctgagt gacctggtag
       61 actttctccc tcgtgaacac ctccaccaac tcagcgtcca gtttcccctc cttggactga
      121 ctctcgagat agccgagtgc cgtgtcagta gagatcgccg ctttgtatgg ccgatcagac
      181 gccgtgaggg cgtcgtagat atcgcaaatc gtcatcatgc gagcctgcag cggaatctga
      241 ttgcttggca gcccacgggg atatccgctg ccatccaact tttcatggtg agtccacgca
      301 atcgacggca cgtttttgag cgacctcgac caagggatct tcgacaagaa ttcaaacgta
      361 tgcgtgacgt gccgctcgat ctcttttcgt tcggactggg tcagactgcc gtggagaacc
      421 gataacgcac ggacttcttc ctcggtcagg aacggacggg gcccgtcata tgaatggtat
      481 tccagacgtg cgatatcctt cagtttcgcg atctgggcag gggattggga actcggctga
      541 ttgcatttca ggataaacga gaacatcttg tctacctcat ccatccgctg tgccagttgc
      601 cggtccagtt gtaccaagag tgcgtgcttt cccgtgcttt tctttgcttc atacacctcc
      661 agctttcgac gtaacgtgtc gacctccatc gtcctcttaa taaaatcgaa ccgcgatttg
      721 acgagttcct gttggagttg atacagcttc cgtgctttca gtagtacgtg ttcacgaacg
      781 ccgaccttgc cgaagtcatg cagcacggcg gcatagcgaa gctctttcag ctgttccgca
      841 ttgaatcgaa tgcccgcata gaggccggaa tcggtttggg tcaccgcgtc cgccagccga
      901 caggtgaggg tggccacacg ttggctgtgc ccgctggtgg agggatcccg gctttcaatg
      961 gcctgcacac tggcggagat aaacccttcg aacaggagat gaatctcctc atagagattg
     1021 gcattttcga tggcaatgcc gacctgatga ccaagcgcga cgaagttctg aagatcgtca
     1081 tccccgaact gttcgccatg tttatgatta atggcttgga gcacacccag aagatggcca
     1141 tgagccgtga caggcacaca gaccatattg cgcgtgacaa atccgctggt ctgatccaca
     1201 tggcggaaga aacgagtatc gtgttggaca tcgttgacga tgatgggctc tccggttcga
     1261 gcgacatgtc ccgctatccc ttgcccttgc cggagacggg cctggcgaac ggcctctccc
     1321 ttctcgccat gggccacatc gaaatacaaa tcaccggtgg cttcatccaa caagagaagg
     1381 gagccggctt cggcattcat caagagcgtg gcggcttcga tggcgcgctt gcggatggtc
     1441 gcgtgatcga gactcgaatt cagcagagcg ctgatttcct gaagctgtcg cagtgagggg
     1501 tctttcatca gataaaatcc agcatgtgtt gctgcacaga atgcctccgt gaaactgtag
     1561 cacgacagat tcgttttgca cggaaaagcg ctgtttagag aggggcaaaa aagcccagga
     1621 tggctaccgc gcaccgcgag acagctggct aaagcatgtc tgctctgggg aaaattggat
     1681 tgcaggacct gaccatcaga ggtccatgcc aatgtagcgg agagttacga acacgacggc
     1741 ccgctgccag ggaaaaggac agcgggtcgt cgacgatgga tacacgttcg agagaaagaa
     1801 ttcagcgctg gcgaccgtta tgcttgtcgc ggaagatgtg cttgctctgc atattctccc
     1861 gatgctggat gcgggcctgt tctcgtgcat tcacatggcc gtctgttccc gcccgcgctt
     1921 ctgtccgatt cacacgggcc tggccctgtc ctattccact tcgatgataa tggtatgggt
     1981 ggccacgacg agatcgtggt ctttcgtggc accagccacg gtgatcttgc gatccccctt
     2041 gctcaggccc ttcagcgtcc cggaaaagcc tttctgatat tcaccatcca cgtacacatg
     2101 gacgtgcgtc gcttgtgtcc ccttggtcag ctcatatttc agctcaaagg tgtcgtcgac
     2161 ttcgtccccg tccttgggag atgtaatgat caccttcgac ccgtcctcga tcggtggttt
     2221 cgtcggttta tcttctgcga agaccggtgc cccgctgaga tagcccgcta gcacgatccc
     2281 taatcccatg atcccgatac gccccatcac agacatcgcg acctccttgt ttgaagcagc
     2341 tgaatcgatg ctgcagctta ccatcttctg cgggatattc tctccccaga ttaccgccgt
     2401 cctcaggtga caaatcgtcg ggggtagatc gcttgctcgg tatgggttgc actctggtac
     2461 atgtcatggg cgacgtcgtg tgcgcagcct gcatcgagca tggtgagcgc aagatcgaca
     2521 ggcacgcgac gtgatacggc catgccatat gggacgcagc ctgattgggt tccctgcccg
     2581 ccgcgacgca ccaaggcctc tttcacatag agccctggtg acgttgaaga tggctgatcg
     2641 caaggctatt ccacttcgac ggtgatggta tccatgggcg gagcaaggct atggttcttc
     2701 atggcagctt tcacggtaat ctcgtgcgtt cccttgctca ggcctttcag caccccagaa
     2761 aatcctctct gatattcatc gtccacatat acatggacat gggtcacttg agatcccttg
     2821 atcaactcgt acttcagctc gaaactgtcg ttcaccttat ccccgtcctt gggagacgta
     2881 atcgtgattt tcgatccatc gactgccgcc ggtctcggtg gttgttcccc tgcgaaaacc
     2941 ggcgccccgc taagatagca cgctaacgcg attcccaatc ctatcgtccc aatacgtccg
     3001 accttatgca tcacgacctc cttgttcaaa accgattcag tggacacaat cacaatttct
     3061 catctgataa cgagccgttt tccggtggag catcttgggg cattctacgt ttcaacgtgg
     3121 tctcaatcaa tccgcacgac acccatcgga tgaccgcagg gtacttgcgc aggctgcgag
     3181 caagaaggat gattggaggt ggcttgagaa acccggcgca ccagggtctc ttacaacaga
     3241 gaccctggtg gcgttgatga tggatgcagc tctattgta
//
LOCUS       DATIPF010000020         9360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_179203, whole genome shotgun sequence.
ACCESSION   DATIPF010000020 DATIPF010000000
VERSION     DATIPF010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9360
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179203"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VLL06_00510"
     CDS             <1..604
                     /locus_tag="VLL06_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HSS29476.1"
                     /translation="GGCSWVPKGASQLDVGIEDRGVASWYGKSFHGKQTANGELFDME
                     ALTAAHRTLPLGTVVRVVNLANGKHLHVRITDRGPYVNSRILDLSRGAAARLGMMDGG
                     LSVVRIQVVGEHRPAALLSSEAMETTSAALTLGLAKAPAGVTESLPSAWGNSDPLLDY
                     SPRLSPGDIWIRQRARRLTGMQAADHTDRTEIATVVSTWT"
     gene            complement(764..1231)
                     /locus_tag="VLL06_00515"
     CDS             complement(764..1231)
                     /locus_tag="VLL06_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29477.1"
                     /translation="MAQKFGNGRWVQEGFLDNRVDGTIVGRIVFAVIGPVDVYLRGNF
                     KADIVGQVIQFRNRRFEDEDLAGQVIGDMENPQLGDVNLISFDPHPNLAPHPYIEWFS
                     TRKNHYRIVLDSEDAWIIPASEMGEIDRVSRTIREALAGRVTEQPTREPTDWM"
     gene            complement(1246..2115)
                     /locus_tag="VLL06_00520"
     CDS             complement(1246..2115)
                     /locus_tag="VLL06_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSS29478.1"
                     /translation="MPRFALLLTTFIWGATFPATKAVLEQLPPLSFLFLRFLLGALLV
                     GGGFLLWRLRLRREPAVLRAGAIATGWLFLGYVLQTVGLHYTTASNSAFITALYVVIV
                     PLILRRFDRRVWVATGIALAGLWLLVKPSASVNVGDLLTLFCAIAFAAHIACLERFTR
                     EVDAPSLFAWQMMAMVVLLLPTLLVEQAPAQAFAPTMVLLVGLGVTGGLATGAFAVQM
                     WVQRIVPAQQVALIFASEPVYAAGLSWYFLGETLDLQGWVGSGLILLSVLVGAFGGGP
                     QRSTPTVARVQPA"
     gene            complement(2213..2422)
                     /locus_tag="VLL06_00525"
     CDS             complement(2213..2422)
                     /locus_tag="VLL06_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29479.1"
                     /translation="MTYWVHIARSIDRVTLHKDRCSEVPSTSIGSSTDFWEGGWFDYP
                     NKERALAAMEQAGTNEQRRCPLCKA"
     gene            complement(2562..2738)
                     /locus_tag="VLL06_00530"
     CDS             complement(2562..2738)
                     /locus_tag="VLL06_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29480.1"
                     /translation="MSLLIRKYKSVSGMMQEERIDDPDRIDRYMRLFEKDDLKKLETG
                     VKVFIEKDEWQLLP"
     gene            2860..3168
                     /locus_tag="VLL06_00535"
     CDS             2860..3168
                     /locus_tag="VLL06_00535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HSS29481.1"
                     /translation="MPIYEYRCGTCEKQFDATQSVHARVEDTVCPHCQAQNATRLLSA
                     STAQIKGTRKPGFTEIKAYNMLDERMEKFAKLPPLMGKRVAPDAAPSSDPSSGGTTEN
                     "
     gene            3240..4061
                     /locus_tag="VLL06_00540"
     CDS             3240..4061
                     /locus_tag="VLL06_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSS29482.1"
                     /translation="MTARLVFSLALSLLLSWGTALVTLAEVSGSLTIAGNGPELTTIE
                     PLARAFEKANPRAYLDILWDGNSKPVEMVKSGQAHIAVTGTEEPGLSATSIAWDGIGV
                     LVHLSNFTKDVTKQQVADIFSGKVTMWSDLGGPETKILVIDRPRNQNIRDAFESQLDM
                     TGKITGAAKVIGSDDQVVKTVVGTLPPLSAVTYLSLSQGLSVVSGGVAVRLLPIDKVE
                     PEVPTVKDGRYPLRRPILLLTKKVPNPLAEAFTQFALSPAGQQLIAETYIPIKEK"
     gene            4093..4551
                     /locus_tag="VLL06_00545"
     CDS             4093..4551
                     /locus_tag="VLL06_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HSS29483.1"
                     /translation="MQNGRSWIFIVLGLFGFLASSITVSYAEESGAIVDGAGFTLYDT
                     ESIKGFDAAKVERDPICDRNKRPRITKVEPDEAKPGDKVVITGENFGTKECFHTVTFS
                     AASNTPVLFKFMSETAIEATVPDAKAGMSFVIIVAGGGSAQSKPVLIKSK"
     gene            4825..6243
                     /gene="accC"
                     /locus_tag="VLL06_00550"
     CDS             4825..6243
                     /gene="accC"
                     /locus_tag="VLL06_00550"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HSS29484.1"
                     /translation="MFKKILVANRGEIAMRIIRACRELNIATAAIYSEADSTGIYVKK
                     ADEAYMVGPGPVKGFLDSHQIVNLALRIGADAIHPGYGFLSENSQFAQLCHTSGITFI
                     GPSPHAIDLMGSKIKARELAKRVGVPIVPGTDGGVTHIKDALLFAKDTGYPVIIKASA
                     GGGGRGLRVVRSDTELRENMAVASREAQAAFGDGIVFLEKYIERPHHIEFQILADRHG
                     NIIHLGERDCSIQRRHQKLIEIAPSLILTPSLRAEMGEAAIAIAKAVDYDNAGTVEFL
                     LDQDSHYYFMEMNPRLQVEHTVTEQITAIDIVRNQIAIAAGLPLEITQQEVTLQGHAI
                     QCRINAEDPKNNFRPSTGTVTAYLSPGGIGVRIDGAVYKDYAVPPYYDALLAKLTVRG
                     RTWEETVSRMRRSLEEYVLRGVKTTIPFMMEIMQDQDFMAGHFDTSYLETHPELFNYH
                     DFEQPEDLVLALSAAIAAYEGL"
     gene            6305..8257
                     /gene="oadA"
                     /locus_tag="VLL06_00555"
     CDS             6305..8257
                     /gene="oadA"
                     /locus_tag="VLL06_00555"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HSS29485.1"
                     /translation="MATRRPSRIQGSKKRTAGPAVRTSKTRARSGQELTIQPATGKPV
                     LITDVALRDGHQSLLATRMRTEDMLPIAQKLDAVGYWSLEVWGGATFDTCLRFLKEDP
                     RERLRALRAAMPNTKLQMLLRGQNLVGYRHYADDVLERFIERSAANGIDVFRIFDALN
                     DIRNVERAMREVKACGKHVEAAISYTVSPVHSVDRFVDMAKTLEDLGTDTLCIKDMAG
                     LLAPLDAYHLVRRIKAAVKVPIHLHSHYTSGMASMTSLMAILGGLDMLDTSISPLAGG
                     TSHPSTETLVASLRNTPYDTGLDLGSFLPITEYFRNVRRKYRQFESEFTGVDAEILTS
                     QIPGGMLSNLAAQLTEQNALDRMKEVLDEVPRVRKELGYPPLVTPTSQIVGTQATLNV
                     LTGERYKVITTETKNYFLGLYGRAPGPLDRDIMARAIGDDQPIKTRPADRLEPELEAI
                     RNDLPAAATTVEDQLSFTLFPAIARDFFEAREKGDLTLEPLEGFSSSGSAAATELHLA
                     PAEFNVTVHGETYHIEVSGSGRKVDGRKPYYIRVNDKLEEVSLEPIQEILAGVPEAPD
                     ASTGAKPKRPRPSKPGDVAPPMPGRVVKVLVKINAVVKAGAPLLIIEAMKMESQVPSP
                     IDGRVTAILVAEGDNVKTDETVIQLE"
     gene            8232..9296
                     /locus_tag="VLL06_00560"
     CDS             8232..9296
                     /locus_tag="VLL06_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSS29486.1"
                     /translation="MKRSSSWSNQTAPHTSGRACGHRYLTLCFVSVLCHIMTACSSPS
                     SIPAQFDAIERMPIHTVLVHDQRIAYLDVGTGPPVILIHGFGGSMWQWEYQQHALAQH
                     FRVLTLDLPGAGLSDKPEIDYRPDQMLDYFVGFMDAVKVPQATLVGNSMGAGLAIGMA
                     LTHPTRVAKLVLIDGLPQHVMEKLTSPSVRRALETSAPSWLVSFGSMLFGGLMTESIL
                     QEIVHDPALLTPAVIERSNRNRQRPGLIKPILAVRENLPLWESEFATRLGEITHPTLV
                     VWGQEDRVFPIAVGEELHRTIKGSRFISIAAAGHIPQWERPDVVTKARSVPALRVKCH
                     SAPSRHAAWPRFPSCVFPSV"
BASE COUNT         2161 a         2874 c         2451 g         1874 t
ORIGIN      
        1 gggtggctgt tcgtgggttc ctaagggggc gtctcaattg gacgttggaa ttgaggatcg
       61 aggtgtggcc tcctggtatg gcaagtcatt tcatgggaag caaacggcca acggcgaact
      121 gttcgacatg gaagcattga ctgcagccca tcgcaccctc ccactcggca ccgtggtccg
      181 ggttgtgaat ttggcgaacg ggaagcatct ccatgtacgg atcaccgacc ggggtcccta
      241 tgtcaacagt cggatcctcg atctttcccg tggcgcagcg gctcgcttgg gaatgatgga
      301 cggtggccta tccgttgtac gtattcaggt ggtcggggaa caccggcctg cggcgcttct
      361 ctcatctgaa gccatggaaa ccacgtccgc cgccctcact ctcggtctcg caaaagcccc
      421 cgcgggggtc accgaatctc tcccttcagc ttggggaaat tcggatccgc tactggacta
      481 ttccccccgg ctgtctcctg gcgatatctg gatccggcaa cgggcccgcc gtttgacggg
      541 gatgcaggcg gcagatcata ccgaccgtac tgaaattgcg accgtcgttt ctacctggac
      601 gtagtgcata tggccggtcg ccggatgtac atgtttcgaa caattgtatg gattgcacga
      661 ctggattgcc ggagcaaaga gtagtgcttg atcgtcattc ttctctcgcg taagggctga
      721 agcctcattc gattcaaaat aggcttcaca gtgtttgagc ccactacatc caatcagtag
      781 gttcccgagt cggttgttca gtgacccgcc cagccagcgc ctcacgaatc gttcgactca
      841 cccgatcgat ctctcccatc tccgatgcgg gaatgatcca ggcatcttca gagtccagca
      901 cgatgcgata atgattcttc ctcgttgaga accattcgat atacggatga ggagcaagat
      961 tcggatgagg gtcgaatgaa attaagttca catccccgag ctgtgggttt tccatgtcgc
     1021 caatcacctg cccggcgaga tcctcgtcct caaaccgacg attccgaaac tgaatgacct
     1081 gccccacgat atcggccttg aagtttcccc gtaaatacac atcaaccgga ccgatcactg
     1141 cgaacacaat ccggcccacg atcgtgccgt caacgcgatt atccagaaac ccttcctgca
     1201 cccagcgtcc atttccaaat ttttgcgcca tcctgacccc ctaatttaag ccggctgaac
     1261 cctggcgacc gtcggcgttg accgctgtgg gccgcccccg aacgcaccca ccaacaccga
     1321 caacaaaatg agtccgcttc ccacccatcc ctgaagatcc aatgtttccc cgagaaagta
     1381 ccaggagagc ccggcggcgt aaaccggttc tgatgcaaaa atcaacgcca cctgttgtgc
     1441 aggtacaatg cgttgcaccc acatttgaac ggcaaaagca ccggtcgcaa gccctccggt
     1501 cacgcccagc ccaacaagca acaccatcgt cggcgcgaat gcctgagccg gtgcctgttc
     1561 caccaaaagc gtgggaagca acaggacaac catcgccatc atttgccagg caaacagaga
     1621 cggcgcatcg acctcgcggg tgaaccgctc gagacaggca atatgtgcag caaacgcaat
     1681 cgcacagaaa agtgtcaaga gatcgccaac gttcacagat gcactgggct tcaccagcag
     1741 ccaaagccct gccagggcaa tcccagtggc cacccacacg cgtcggtcga atcggcgcag
     1801 aatcaatggc acgatgacga catacagtgc tgtaatgaat gctgagttgg acgctgtcgt
     1861 atagtggaga ccgaccgtct ggaggacata gccaagaaac agccagccag tggcgatcgc
     1921 accggcccgt aaaaccgccg gttcgcgccg caagcgcaat ctccacagga gaaacccgcc
     1981 cccgaccaac agtgccccca ggagaaatcg gaggaacaga aacgacagtg gtggaagttg
     2041 ctccaacacc gctttggtgg cagggaacgt cgcgccccaa ataaatgttg tcagcaggag
     2101 tgcgaatcgt ggcatggagt cacgtgataa acgcggagcg ccaagagatg tgtcaccagg
     2161 attcggtcat cgcaccaatc catgatcgcc gagtcttcgt cgctcgtcac tgctaggctt
     2221 tgcacaacgg acaacggcgc tgctcattcg tcccggcctg ctccatcgcc gctaacgccc
     2281 gctccttgtt cggataatca aaccacccgc cttcccaaaa atcggttgaa gaaccgatcg
     2341 acgtagacgg tacctcagaa caacgatcct tgtggagcgt gacgcggtcg atcgaccgtg
     2401 cgatgtgaac ccaataggtc atagaaacag ctcctcggag gcagaccacc acacagccct
     2461 tcgctcgtga ctccaggccc gaacacactc atgaccagca atcggattct gattcgatct
     2521 acggtggagt cttgtctacg aacgggatgt gcaacacaac gtcagggcag gagttgccac
     2581 tcatccttct ctatgaagac cttgacgccc gtctcaagct tcttaagatc gtccttttcg
     2641 aagagtctca tataccggtc gatccgatcc ggatcgtcga tccgctcttc ctgcatcatt
     2701 ccactcacac tcttatactt ccgaatcaac agggacatcg aaatcctcct ctttgccact
     2761 agaagtcatc tcagctcagt gtgtagaata aggcaaagct taacgaccgg tcaagagcgc
     2821 gtgcttggtt tcccgtcatc ccgcttgagg atacgcaata tgccgatcta cgaatatcgt
     2881 tgtggtacgt gcgagaagca gtttgacgcc acccaatccg tccatgccag agtggaagac
     2941 accgtgtgcc ctcactgtca ggcccaaaac gccacgcggc tcctgtccgc cagcacggca
     3001 cagatcaaag ggacccgcaa acctggcttc accgaaatca aggcttacaa catgttagac
     3061 gagcggatgg aaaaatttgc caaacttcca ccgctgatgg gcaaacgcgt cgcccctgac
     3121 gcagccccat cttccgatcc ctcttccgga ggcacaacgg agaactgacc aaaccgtagc
     3181 ctataggtga acacacaccg ccctcttctt aacatccacc cctacccctt ggagactgca
     3241 tgactgcgag actcgtgttc tccctggctc tcagccttct cctcagctgg ggaacggccc
     3301 ttgtcaccct tgcggaggtt tcaggaagtc tcaccattgc cggcaacgga ccggaactga
     3361 cgacgatcga gcctctcgcc cgcgcatttg aaaaagccaa cccgagagcc tatcttgata
     3421 tcctctggga tggcaattca aaacccgttg agatggtgaa atccggccaa gcccacatcg
     3481 cggtgaccgg aaccgaagag ccaggcctct cggcgacgtc catcgcgtgg gatggaatcg
     3541 gcgtcctcgt acacctctcc aatttcacga aagacgtcac aaagcaacaa gtggccgaca
     3601 ttttctccgg aaaagtcacg atgtggtcag acctcggcgg tcctgagacg aaaatattgg
     3661 tcatcgaccg gcctcgaaac caaaacatcc gcgatgcatt tgaatcacaa ctcgacatga
     3721 ccggtaagat tacaggtgcc gccaaggtga ttggctcaga tgaccaggtc gtcaagaccg
     3781 ttgtgggcac actgccccct ctgtctgcag tcacctacct gtccctgagc caggggttgt
     3841 ccgtcgtctc aggcggcgtt gccgtaagac ttttgcccat cgacaaggtt gaaccggagg
     3901 tccccaccgt caaagacggc cgctatccgc tccggcgccc tattctgttg ttgaccaaaa
     3961 aggtgccgaa ccccctggct gaggcgttca ctcagttcgc cctctctccg gccggtcaac
     4021 agctcattgc cgaaacatat attccgatca aagagaaata atcatgtctc attgttcttg
     4081 aaggaggggc caatgcagaa tgggcgatcg tggattttca tagtattggg actcttcggg
     4141 ttcttggctt catccatcac tgtatcctat gcggaagagt caggcgcgat cgttgacggc
     4201 gcaggattca ccctctacga cactgagtcg attaagggat tcgatgccgc aaaggtcgaa
     4261 cgggatccca tctgcgatcg gaacaagcgc ccacggatta ccaaagtcga gccggacgaa
     4321 gccaaaccag gcgataaagt cgtcatcacc ggcgagaatt ttggcacgaa agaatgtttt
     4381 catacggtga ccttcagcgc ggcttcaaat acgcccgttc tgttcaaatt catgagcgag
     4441 acggcgattg aagccaccgt accggatgcc aaagcgggga tgtcgtttgt tatcatcgtt
     4501 gccggaggcg gaagcgccca gtcaaaacct gtcttgatta agtccaagta ggcgtgctcc
     4561 attgagaacg cgagccggcg cggttctgtt ggcaatcttg gtatctcgcc catcccacct
     4621 ttcccgcgct tgccatacgt cgctcggacg tctcgcgtag cacagggcag tgccagcgga
     4681 aaaccgcatc aacattccct aagatccaat ctgttcgact ccacctccct cgacaggact
     4741 cctgacccta tgctagctta cgcgctcatt ttcgcctgat tactctcgca gaatcgcgaa
     4801 cgatctgagg agatacggac cggtatgttc aagaaaattc tggtcgccaa tcgcggcgaa
     4861 atcgccatgc gaattatccg cgcctgccgt gaattgaaca tcgccaccgc agccatctat
     4921 tccgaagccg attcgaccgg gatctacgtc aaaaaggccg acgaagccta catggtggga
     4981 ccgggaccgg tgaaaggctt cctggatagt caccaaatcg tcaacctggc actacggatc
     5041 ggcgctgatg ccattcatcc aggctatgga tttctctcgg aaaactcgca atttgcgcag
     5101 ctgtgccaca cctccggtat taccttcatc ggcccgtcac cacatgccat cgacttgatg
     5161 ggaagcaaga tcaaagcccg cgaattggcc aagcgagtcg gggtaccaat cgtccccggt
     5221 accgacggcg gcgtcaccca catcaaggac gccctgttat ttgcaaaaga taccggctat
     5281 cccgtcatca ttaaagccag cgcaggcgga ggaggacgcg gccttcgggt ggtgcggtcg
     5341 gataccgaac ttcgcgagaa catggccgtc gcatctcggg aagcccaggc tgccttcggt
     5401 gacggaatcg tcttccttga aaagtatatc gagcgaccgc atcacatcga attccagatc
     5461 ctggccgatc ggcacggcaa catcatccac ctgggcgagc gcgattgttc gattcagcgc
     5521 cggcatcaaa agctcatcga gatcgcgcca tccttgattc tcacgccaag tcttcgtgct
     5581 gaaatgggtg aggcagccat cgccatcgcc aaggcggtcg attatgacaa tgccggcacc
     5641 gtcgagttcc ttctcgatca ggacagccac tattacttca tggagatgaa cccgcgactc
     5701 caagtcgaac atactgtgac tgaacagatc acggcgatcg acatcgtgcg caatcagatc
     5761 gcgattgcag ccggcctccc acttgagatc acacagcagg aggtcaccct ccaaggccat
     5821 gccattcaat gccgcatcaa tgcggaagac ccgaagaaca acttccggcc ttccaccgga
     5881 accgtcacag cctatctctc gccgggcgga atcggggtgc gcatcgacgg agccgtctat
     5941 aaagactatg cggtgccgcc ctactacgac gcgctcttgg ccaagctcac ggtccgcggc
     6001 cgtacgtggg aagaaacggt cagccgcatg agacggtcgc tcgaagagta cgtcttacgc
     6061 ggggtgaaga cgacgatccc ctttatgatg gagatcatgc aggaccagga cttcatggca
     6121 ggacatttcg acacgtccta tctagagacg catcctgagc tattcaacta ccacgacttt
     6181 gagcagccgg aggatctggt gcttgcactc tctgccgcta tcgccgccta tgaaggacta
     6241 tagccgaatc actgcctatc aacccagtga tccgatgatg agataaggca tagatcagga
     6301 cattatggca acacgacgac cctcacgaat acagggctcc aagaagcgga cagccgggcc
     6361 agccgtccgg acgtcgaaga cacgtgcccg cagcggccaa gaactgacga ttcaaccggc
     6421 gacgggcaag cccgtcctga tcaccgatgt cgccttgcgc gatggccatc agtcgctctt
     6481 ggccacgcgc atgcgaacgg aggacatgct gcccatcgcc caaaagctcg acgccgtcgg
     6541 ctattggtcg ctcgaggtct ggggtggcgc caccttcgac acgtgcctcc gttttctcaa
     6601 agaagatccc cgggagcggc ttcgggcgct acgtgctgcc atgcccaata ccaagctcca
     6661 aatgttgctc cgcgggcaga acttggtcgg atatcgacac tatgccgacg atgtgctgga
     6721 acggttcatt gaacggtccg ccgccaatgg cattgacgtc tttcgcatct tcgacgccct
     6781 gaacgatatc cggaacgtcg aacgggccat gcgggaagtg aaagcctgcg ggaaacacgt
     6841 cgaagccgcc atcagctata ccgtcagtcc cgtccacagc gtcgaccgtt ttgtcgacat
     6901 ggccaaaacg ctggaggatt tgggaacaga cacactctgc atcaaagaca tggccgggct
     6961 cttggctccg ctcgacgcct atcacctcgt acgccggatc aaagcggcag tcaaggtccc
     7021 catccatttg cattcacact acacctcggg aatggcctcg atgacctctc tcatggcgat
     7081 cctcggggga ctggacatgt tggatacgtc catctcgccg ttggccggcg gcacttcgca
     7141 tccgtcgacg gagacgctgg tggcctcgct gcgcaacacc ccatacgaca cggggctgga
     7201 ccttggatcg ttcctcccga tcaccgaata cttccgcaac gtacgacgaa agtatcggca
     7261 gttcgaaagc gagttcaccg gggtcgatgc cgagatcctc acttcccaga ttcccggtgg
     7321 catgctctcg aatcttgccg ctcaattgac tgaacagaat gcgctcgatc ggatgaaaga
     7381 agttctggat gaagtgccgc gtgtccgaaa ggaactgggc tatcccccgc tggtcacccc
     7441 aaccagtcag atcgtaggca cccaagccac gctcaatgtc ctgacaggcg aacggtataa
     7501 agtcattacc acggaaacca agaactactt tctcggactc tacggacggg ccccagggcc
     7561 tctcgatcgc gacatcatgg cccgcgcaat cggggacgac cagccgatca agacgcggcc
     7621 cgccgatcgc ttggagcctg aactcgaggc catcagaaac gatctgcccg ccgcggccac
     7681 cacggtggaa gatcagctgt cgttcaccct gttccctgca atcgcgcgag acttctttga
     7741 ggcgcgcgag aaaggagact tgacactgga gccccttgaa ggcttctcgt cgagtggatc
     7801 cgccgcggcc accgaactcc acttggcccc ggcagaattc aacgtcacgg ttcacggtga
     7861 aacctaccat atagaggtat ccggttccgg acggaaggtc gacggccgta aaccctatta
     7921 catccgagtc aacgacaaac tcgaagaagt ttctttggag ccgatccagg agattctggc
     7981 cggagtgccg gaagctcccg acgccagtac tggcgccaaa cccaaacggc ccaggccatc
     8041 aaaaccaggg gatgtagccc ccccaatgcc tggtcgcgtc gtcaaagtgc tcgtcaagat
     8101 caatgccgtg gtcaaggccg gtgcccccct gctgatcatc gaagccatga agatggaaag
     8161 tcaggtcccg tcgccgatcg acggaagagt caccgccatc ctggtcgccg agggcgacaa
     8221 cgtgaagacg gatgaaacgg tcatccagtt ggagtaatca gacggccccg catacctccg
     8281 gtcgtgcatg cgggcatcga tatttgacgc tgtgttttgt gagtgtgttg tgtcacatca
     8341 tgaccgcctg ctcctctcca tcgtcgattc cagcgcagtt tgacgctatt gagcgcatgc
     8401 ccattcacac cgtcctggtc cacgaccagc ggatcgcgta tctggatgtc ggcacaggcc
     8461 cgccggtcat cctgatccac ggtttcgggg gatcgatgtg gcaatgggaa tatcagcagc
     8521 atgcgctggc gcagcatttc cgcgtcctca cgctcgattt gcccggcgca ggattgtccg
     8581 ataaacctga gatcgactac cggccggatc agatgttgga ctactttgtc ggattcatgg
     8641 acgccgtgaa ggttccgcaa gccacgttag tcgggaattc catgggcgcc gggttagcca
     8701 tcggcatggc tctgacacat cccacacggg tcgccaagct cgtcctgatc gacggcttac
     8761 cgcaacacgt catggagaaa ctcaccagtc cgtcggtcag gcgggcctta gaaaccagtg
     8821 cgccgtcttg gctcgtctca ttcggtagca tgctgttcgg cggactgatg accgagtcca
     8881 tcttacaaga aatcgtccat gacccggcac tgctcacacc agccgtcatc gaacgatcca
     8941 atcgaaaccg tcaacgcccc ggactcatca aacccatcct cgccgtcaga gaaaaccttc
     9001 cgttatggga atccgaattt gcgacacgcc tcggtgaaat tacccaccca acgctggtag
     9061 tctgggggca agaggatcgg gtgtttccaa ttgccgtagg cgaagaactc caccggacca
     9121 tcaaggggtc acgattcatc agcatagctg ctgcaggaca catcccgcag tgggaacggc
     9181 cggatgtagt cacgaaagca cgatctgttc ctgcgcttcg cgtgaaatgc cattccgctc
     9241 catcccggca cgcagcttgg cctcgatttc cttcatgcgt ttttccgagc gtttgaatcc
     9301 catggcgcgg cgcaaatctt ctgcctcacc gccactgaag ttggcgacca ccatggccat
//
LOCUS       DATIPF010000021         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1063657, whole genome shotgun sequence.
ACCESSION   DATIPF010000021 DATIPF010000000
VERSION     DATIPF010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063657"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VLL06_00565"
     CDS             <1..330
                     /locus_tag="VLL06_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS29487.1"
                     /translation="LIIRTRAVSSTELPGTVIVSEGALVNGTSVRLIIADTGIGIPPE
                     HLTRIFEPFFTTRHDQGGTGLGLAICHRVVTDSGSRLAVKSEVGHGTEFTVDLPIWNE
                     GEIRRHQ"
     gene            336..1736
                     /locus_tag="VLL06_00570"
     CDS             336..1736
                     /locus_tag="VLL06_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSS29488.1"
                     /translation="MPSILVVDDDKPNREMLAEALSQAGFAVDMACDGIEALSKANAN
                     AGTYDTVLSDIRMIPVSGLDLLDSFRKTMPEMPIVLLTAFGSVDTAIQAMKQGAFDYI
                     AKPVNLEELVLTMKRAVEYRRLIKNNHTPKRAFSERSRAASLIGQSKQMVEVFKLIGK
                     VSPSRTAVLIQGESGTGKELIARAIHDNSPRATSPFVPVNCSAIPDTLLESELFGHTK
                     GSFTGAHTMRRGLLEDASGGTFFLDEVGDLSPAGQAKLLRVLQEGEIRRIGSNEAVRV
                     DLRVIAASRRNLAQLVAIGRFREDLLYRLNTVTILLPPLRERHEDVPLLAEFFLARYG
                     DKKEVPVTSFSSATMRALTEYSWPGNVRELEHVVERAVALASQAVLSLDDLPPEVIQK
                     EGEASDQSDILPGTLKALQREQVLKMLETTHGNKERTARLLGISRRTLYRLLEQYGLA
                     KPKTSTAADTSDPVGS"
     gene            1843..2436
                     /locus_tag="VLL06_00575"
     CDS             1843..2436
                     /locus_tag="VLL06_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HSS29489.1"
                     /translation="MMKGLWLGLVVGITFTCLLGQSGFSAASKETDSPKGISPEIVAD
                     YVHSVVQADRTFYQNEIVGRMREKGMVSASEHWRADGGLPLPVQFLQESGRLMAKQGK
                     MIRFRLISDWAINKESGPTTEFERTGLMKVLENPDRPYTAMTTEGKARRFQALYADKA
                     VSQFCADCHNVHPKSPKRDFKAGDVMGGMLITIELPQ"
BASE COUNT          617 a          668 c          708 g          551 t
ORIGIN      
        1 ttaattatcc ggacccgtgc tgtctccagt acagagcttc ctggaacagt catagtaagt
       61 gaaggtgcgc tagtcaatgg cacttcggtc cgcttaatca tcgccgacac gggcattggg
      121 attcctccag aacacttaac ccgaatattc gagccgtttt tcacaacccg acacgatcaa
      181 ggaggaacgg gattggggtt ggcgatctgt caccgtgtag tgacagacag cggcagcagg
      241 ctcgccgtga agagtgaagt gggacacgga acggagttca ccgtggatct gcccatctgg
      301 aatgaggggg aaatccggag acatcaatga ggggtttgcc atcaattctc gtcgtggatg
      361 acgacaagcc gaaccgggag atgttggccg aagccttgag ccaagccggc tttgcggtcg
      421 acatggcctg tgatggcata gaagccctga gcaaggccaa tgccaatgcg ggaacgtacg
      481 atactgtatt gagcgatatc cgcatgatcc cggtttctgg tttggacctt ttagattcgt
      541 ttcgcaagac catgccggag atgcccatcg tgctcttaac cgcgttcggc tcggtcgaca
      601 ctgccatcca agccatgaag caaggagcat tcgattacat cgccaagcct gtgaatcttg
      661 aggaactcgt cctcaccatg aaaagagccg tcgaataccg ccgtctgata aagaacaatc
      721 atacgcctaa gcgcgccttt agtgaacggt cgcgggccgc ctctttgatc ggacaaagca
      781 agcaaatggt cgaggtcttc aaacttattg gaaaggtttc tcctagccga acagccgtat
      841 tgatccaggg tgagagcggc accggtaaag aattaatcgc ccgcgccatt cacgataaca
      901 gcccgcgtgc gacctccccg ttcgtccccg tcaattgtag tgcgattccg gataccttgc
      961 tcgaaagcga actcttcggt cacaccaagg gttcgttcac cggtgctcac accatgcggc
     1021 gtggattgct tgaggacgcg agcggtggca cgttcttttt ggacgaagtc ggcgatctct
     1081 ctccagccgg acaggcaaaa cttctccgcg tcctccaaga aggtgaaatc cgaaggattg
     1141 gaagcaacga ggcggtacgg gtagatttgc gggttattgc agcctcgcgc cgaaatctcg
     1201 cgcaactggt agcgatcggc cggtttcgtg aagatctgct ctaccggttg aacacggtca
     1261 ccattctcct gccaccgctt cgggagcggc atgaagatgt tcccttgctg gcagaattct
     1321 ttctcgcacg gtacggcgac aagaaggagg ttcccgtcac gtccttttca tccgccacga
     1381 tgagggcgct gactgaatat tcctggccgg ggaacgtgcg tgagttagaa cacgtcgttg
     1441 aacgagcagt ggcgctggcc tcgcaggctg ttctctcact cgacgatctt ccacctgaag
     1501 tcattcagaa agagggagaa gcttcggacc agagtgacat cctccccggc acgctcaaag
     1561 cgctgcagcg ggaacaagta ctcaaaatgc tagaaacaac ccatggcaac aaagagcgca
     1621 cggctcgttt actcgggatc agcagaagaa cgctgtaccg gctcctcgaa caatacgggc
     1681 tcgccaaacc taaaacttcg accgcagctg acacttcgga tcctgtcggt tcctgatcct
     1741 cgtcgcccat catttcgacg gagtttctat cattttagtg ggtagcagag cctaagagcg
     1801 aatcaagtca caagtagaac aagaaatgga ggacgcatcg tgatgatgaa gggtctttgg
     1861 ttggggctcg ttgtgggaat caccttcacg tgcttactcg gtcaatcggg gttctctgcc
     1921 gcgagtaagg agaccgattc acccaagggc atatcccccg aaattgtcgc cgattatgtg
     1981 catagtgtgg tccaggcgga tcgaacgttt taccagaacg agattgtagg tcggatgagg
     2041 gagaaaggta tggtttctgc ctcggaacat tggagagcgg acggagggct ccctttacct
     2101 gtccaatttt tacaggaatc ggggcggctc atggctaaac agggaaaaat gattcgattc
     2161 cgcctgatca gcgattgggc gatcaataaa gaaagtggcc ctacgacaga gttcgagcgg
     2221 accgggctta tgaaggtcct cgagaatccc gatcgtcctt ataccgccat gacaaccgag
     2281 ggcaaggctc gccgcttcca agcactctat gccgataaag ccgtgtctca attctgcgcc
     2341 gattgccaca atgtgcaccc caaaagccca aagagggatt tcaaggcagg cgacgtgatg
     2401 ggtgggatgc tgatcaccat tgagctcccc caataggaaa ggccaaccga agataagcgg
     2461 ctttcatgga cgctgcatgt gggggccagc aagagagaac cgttcccata gatttcgttg
     2521 atcgtttgat tagtcaaaat cact
//
LOCUS       DATIPF010000022         6638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1506194, whole genome shotgun sequence.
ACCESSION   DATIPF010000022 DATIPF010000000
VERSION     DATIPF010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6638
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506194"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="VLL06_00580"
     CDS             complement(<1..1313)
                     /locus_tag="VLL06_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HSS29490.1"
                     /translation="MITADVKPHSQPTSPALTMLLQLEGMVRAVHTQQELQFFFVNET
                     RRLVPYQQAILLSPPTPSTQRYQVHAASSVPVVDRTVPLMQWTERLIQELRQTSTGPG
                     IRHVTEAACPADLRGDWKEFTPGYGLWCPLTHPRGDILGGLWLTRDQPWADYEETVLQ
                     RLSEAYAYAWRAVGPSNHRRWRWELSRTVTWSLAAVTLGALAIPVPMSTLAPAKIVAK
                     DPMIVSAPIDGVITDILVLPNTVVSEGQVLFKYEDTTFRSEYDAAERNLDVAMAQYRR
                     AAQGSFVEAAQQADVPLLKAEVELRETERNYAHERLSKVEVKAEQAGLLLYTDKSDWI
                     GKPVVIGQRIMELADPHQLEVRIDLPVEDAIVLREGATVTLFLHANPFSSVPATVSHA
                     SYHAEVLPNHTLAYRVTAQLEQGSSEFRIGWQGSAKIYGERVTLFT"
     gene            complement(1316..2104)
                     /locus_tag="VLL06_00585"
     CDS             complement(1316..2104)
                     /locus_tag="VLL06_00585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSS29491.1"
                     /translation="MTRICWLLVAGALASGGMLVPLWLATAGPLSEPKPASSAAELST
                     IRGVVKATAQATLASQVQGRINQLPVKEGQRFKKGALLVALDCAKYEAELASAQAEHR
                     GKQKTYENNLRLSQHASVGQLELDLSQAEAEKALAAVKGAHVNVNGCTVRAPFPGRVV
                     KMIVNEHENVFPNDQLLCLLDDSLLEIALILPSKSLAWLKVGAPFEYVVDETGLRYPA
                     AIQDIGASVDPASQTVKVKGLFRTQPDNVLAGMSGTAVFPELPR"
     gene            complement(2101..3636)
                     /locus_tag="VLL06_00590"
     CDS             complement(2101..3636)
                     /locus_tag="VLL06_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSS29492.1"
                     /translation="MNSPIASSQRARLPPRPCTTGLLLVTMMGLTSCAITPIPLTTNE
                     VQSRAKADRAVLTQEQEPVSGPIDVYEAMARALKYNLDARVELMHKMLAQTQLDLSHY
                     AMLPRLAANAGFDGRSNFSGGQARSLITGQQQIQPFTSSEKNVFSADLSLSWNVLDFG
                     LSYIRAKQAADDVLIAEEERRRVANRVMQDVRSAYWRAVSAERILPSLKMLDEWVKNA
                     LDKAQTIQDEKLSTPLVPLQYRLDLLNTQRYIQQLYRELLTAKLQLAALINLPPGQEH
                     AMKLIVPEREASTLNLPLDMTGLEDRALEARPELRMIDYRRRINARETKAAILEMLPS
                     LNLQAGGNYNSNSFLFNQNWTAYAARTSWNLLSLFRYPARAKTIEAQDTVLHTQNLAL
                     TMAIMSQVHVSVAQVAQAKKETSTAKLYHETQSQISEQTRLGWRTGRQSEQAVLRERV
                     NQVSAQLRLDVMESEVQVSWAMLLAAVGEDVLPNDLTREQSVSDLALELRTRWAKSKE
                     LLQ"
     gene            complement(4071..4745)
                     /locus_tag="VLL06_00595"
     CDS             complement(4071..4745)
                     /locus_tag="VLL06_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HSS29493.1"
                     /translation="MGTSRNPALFTPTKTKRERIVVGASTGGVEALQRFFAALPATFP
                     AVVGAVLHRGSSPTSRLASVLARGAALPVREPHDGETLQRGTIYLAPADHHMVFHDGY
                     VSVLHDPKESYARPSIDRLFRSAAIAYGERVVGVLLSGAGTDGVRGMRAIIAAGGLCL
                     VQDPHEAAMPSLLLNVLRNAHVDGIFLIDDLASMLEVLVRGKQTVRARKPMIDGHMPA
                     NERGIE"
     gene            4823..5263
                     /locus_tag="VLL06_00600"
     CDS             4823..5263
                     /locus_tag="VLL06_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS29494.1"
                     /translation="MPHGPDIPPTTSVLLIDEREADRTHWAVQLKQCSPDYEIVEATD
                     GQSGLDVCKSRRIDCVVLEIELPDISGFEVLTTLVPRANKPNMAVIVLTQLMHRGVRE
                     LARTNGAYVCFVKRHTTGEDLDRAIQRAVALVGQMPKEDRYRNI"
     gene            complement(5295..5588)
                     /locus_tag="VLL06_00605"
     CDS             complement(5295..5588)
                     /locus_tag="VLL06_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29495.1"
                     /translation="MSTENNAHIEANLEVIRLYLIGQFKGFELTDTPNHPLSHTFTAM
                     KSADERYQVKVSWAQLSDISNTPERTKKRLVTDDVAGRMKGKSQGEYFSWGKY"
     gene            complement(5599..5862)
                     /locus_tag="VLL06_00610"
     CDS             complement(5599..5862)
                     /locus_tag="VLL06_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29496.1"
                     /translation="MAHFGPKQENEYQRKRSLEREVLEHLKLYSPKKWDTLYIHFAID
                     RQTNIQPVLHALEKAGHIEVGNGKDQMVAITGSGLKQLEKPDF"
     gene            complement(6375..6530)
                     /locus_tag="VLL06_00615"
     CDS             complement(6375..6530)
                     /locus_tag="VLL06_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29497.1"
                     /translation="MTKVQATYLQATWNEQGFPLCEHLKIDLERSEDGFMTGNYRCLA
                     CGDAVAR"
BASE COUNT         1306 a         1940 c         1907 g         1485 t
ORIGIN      
        1 gtgaagagtg tgacccgctc tccgtatatt ttggcactgc cttgccagcc gatgcggaat
       61 tccgacgacc cctgctcgag ctgtgccgtc acgcggtagg ccagcgtatg gttgggcaag
      121 acctcggcat ggtaactcgc atgactgact gtagccggca cactggaaaa gggattggcg
      181 tggagaaaca aggtcaccgt tgcgccttcg cgcaacacaa tcgcatcctc caccggcaga
      241 tcgatccgca cctccagctg atgcgggtcc gctagttcca tgatacgttg gccgatcacg
      301 accggcttcc cgatccaatc ggatttgtcg gtatagagca acagcccggc ttgctccgcc
      361 ttcacctcga ccttcgacaa gcgctcatgg gcatagttcc gctcagtttc gcgtaactcg
      421 acctccgctt tcagcagcgg cacatccgct tgttgcgcgg cttccacaaa ggagccttgc
      481 gccgcacgcc gatactgcgc catcgcgacg tcgaggttcc gctcggcggc gtcatactcg
      541 cttctgaatg tggtgtcttc atacttgaac aatacttggc cttcggacac gacggtattc
      601 gggaggacga gaatgtcggt aatgactccg tcgatcggcg cgctgacgat catcggatcc
      661 ttggcgacaa tcttcgccgg cgccagggtc gacatgggaa caggaatggc cagggccccc
      721 agcgttacgg ccgccaggct ccacgtcacg gtccgactca gttcccatcg ccagcgacga
      781 tggttcgagg gaccgacagc acgccaggcg taggcgtagg cctcgctgag acgttggagg
      841 accgtctcct cataatccgc ccatggctgg tcccgtgtca gccacagacc tccgagaatg
      901 tctcccctgg ggtgcgtgag cgggcaccac aatccatagc ctggagtaaa ttccttccag
      961 tcccctctga ggtcagcagg acacgccgct tccgtgacgt gacgaatgcc cggacccgtc
     1021 gaggtctggc gcaactcctg gatcaatcgc tcggtccatt gcatgagggg cacggtgcga
     1081 tccacgaccg ggacgctcga cgcggcgtgc acctgatacc gctgcgtcga tggggtcggc
     1141 gggctgagca gaatggcttg ttgatagggc accaatcgac gcgtttcatt gacgaaaaaa
     1201 aattgcagtt cctgctgggt gtggacggcg cgaaccatcc cttccagctg aagcagcatg
     1261 gtcagggctg ggctcgtcgg ctgactgtgt ggtttgacgt ctgcggtgat catcttcagc
     1321 gtggaagttc ggggaacacc gcggtcccgc tcatacccgc caggacattg tcgggctggg
     1381 tgcgaaacag gcctttcacc ttcaccgtct ggctcgccgg atcgacactc gcaccgatgt
     1441 cctgaatcgc ggcgggatat cgaagtcctg tttcatccac aacgtactca aagggtgccc
     1501 cgaccttcag ccacgcgagg gacttcgacg gcagaatgag cgcgatctcc agcaggctat
     1561 catccaacag acataggagt tggtcgttgg gaaacacatt ctcatgttcg ttcacgatca
     1621 ttttgacaac ccgtccgggg aacggggcgc ggacggtgca gccgttcacg ttaacgtgcg
     1681 cacccttcac ggcggcgagc gctttctccg cctcggcctg cgagaggtcc agctcgagct
     1741 ggcccaccga cgcgtgttgc gacagacgga ggttgttctc ataggttttc tgcttgccac
     1801 ggtgttctgc ctgggcgctt gccagttccg cctcatactt tgcacagtcg agcgcgacta
     1861 ggagcgcgcc ttttttaaac cgctgtccct ccttgacggg cagctggttg atccggccct
     1921 gcacctggct ggccaaagtg gcttgtgccg tcgccttcac cacgccacgg atcgtgctga
     1981 gttctgccgc gctgctggcg ggcttgggct ctgacagagg gccggccgtc gcgagccaca
     2041 gcggcaccaa catcccccca ctcgcgagcg cgcccgcgac gagtaaccag cagatccggg
     2101 tcactggagc aactccttgc tcttcgccca tcgcgtccgc agttcgagag ccaggtcgga
     2161 gacgctctgt tcccgcgtca gatcgttagg cagcacatct tcgccgacgg cagcgaggag
     2221 catggcccag gacacttgca cttctgattc catgacatcc aggcgcagtt gcgctgatac
     2281 ctggttcact cgctcgcgca ggaccgcctg ttcgctctgc cgtccggttc tccatcccag
     2341 gcgcgtctgc tcggagattt gggactgcgt ctcgtgatac agcttcgccg tcgaggtttc
     2401 ctttttcgct tgtgccacct gcgccacgct gacatgcact tgcgacataa tggccatagt
     2461 cagcgcgagg ttctgcgtgt gcagcaccgt atcttgcgcc tcgatggtct tcgcccgagc
     2521 cgggtaccga aacaagctca gcaaattcca actggtccgg gcggcatagg ctgtccaatt
     2581 ctgattgaag agaaatgagt tgctgttgta gttcccaccg gcttggagat tcaggctggg
     2641 taacatttcg aggatcgcgg ctttggtctc gcgcgcgttg atgcgacgac ggtaatcaat
     2701 catccttaac tccgggcgtg cttccagtgc ccggtcctcg agccccgtca tatcgagggg
     2761 caggttcagc gtgctcgcct ctcgctccgg cacgatgagc ttcatggcgt gctcctggcc
     2821 ggggggcaga ttgatgagcg cggccaattg aagcttcgcc gtcaggagtt cccgatacag
     2881 ctgttggata tatcgctgcg tattgaggag atccaaccgg tactgcaagg gaaccagtgg
     2941 cgtgctgagc ttctcgtcct gaatggtctg ggctttgtcc agggcgttct tgacccattc
     3001 atcgagcatc ttgagggagg gaagaatgcg ttcggcgctg accgcgcgcc aataggcgct
     3061 tctcacatct tgcatgacac gattggcgac acgccgacgc tcttcttcgg caatcaacac
     3121 gtcgtcggct gcctgcttgg cgcggatgta ggacaatccg aaatccaaca cattccagct
     3181 gagtgacaga tctgccgaga acacgttctt ttcggaagag gtaaagggtt ggatttgctg
     3241 ctgccccgtg atcaatgatc gcgcttgacc cccgctaaaa ttactgcggc cgtcgaatcc
     3301 tgcgttcgcc gcgagacgag gcaacatggc atagtgcgac aggtcgagtt gggtctgggc
     3361 cagcatcttg tgcatcagct cgacgcgtgc gtcgaggtta tacttcagcg cccgcgccat
     3421 tgcttcgtaa acatcgatcg ggcccgagac tggctcctgc tcctgggtca gcaccgcccg
     3481 gtctgctttc gcgcgtgatt gcacctcgtt cgtcgtcaac ggaatcggtg taatcgcaca
     3541 actggtgagc cccatcatcg tcacgagtag cagccccgtc gtacacggac ggggagggag
     3601 gcgtgctcgt tgagacgaag cgatcggact gttcacgacg taattccctg ctcaggtaga
     3661 gtcgttgccc ccgatcggtg cgcgcacacg ggcagaccgc ggagcgcagc tgcccgatca
     3721 ccgatcggct tcatgcctac gtaatggttc tagagtactc gcctgctgcg gcttgtcacg
     3781 atgtttctgg cttttgcaca gcacgagccg tgctcattct gatcgccaag atccactcgc
     3841 acgacgggtc atcggcacca cacgatccag ttcctccacc ttcacagtca tccttgcgct
     3901 cgctgggcca tccatgtggg tgcttgcgtg gccgctctgt gcgcgggtgt gtttgtagga
     3961 gggatgcgct agacgatcag gcgagattcg cgggaaaatg gttagattta gcggggcaat
     4021 gacttcccct cacgatgtct cttctcactg tcttccgtag tccgctttcg tcactcgatg
     4081 cccctctcat tggcaggcat atgcccatcg atcatcggct ttcttgctct cacggtctgt
     4141 ttgccgcgca ccagaacctc caacatcgat gccaagtcgt ctataagaaa tatcccgtct
     4201 acgtgagcat tgcggaggac attgagcaac agagaaggca tggcagcctc gtgaggatct
     4261 tggaccaggc acaggccacc agctgctatg attgcccgca tcccgcgcac tccgtctgtt
     4321 ccagcccctg agagtaacac gccaacgaca cgctccccat acgcgattgc ggcagaacga
     4381 aacaatctgt cgatcgatgg gcgcgcatag ctctcttttg ggtcgtgcag gaccgacacg
     4441 tatccgtcgt gaaacaccat gtggtggtcc gctggggcca ggtaaatggt cccgcgttgc
     4501 aacgtctcac catcatgagg ttcgcgcact ggtagtgctg caccacgcgc caacacagac
     4561 gccagccgac tcgtcggcga ggatccgcga tggagtaccg caccgacgac cgccgggaat
     4621 gtggcgggga gtgcggcgaa gaacctctgc aaggcctcga cgcctccagt ggaagcgcca
     4681 acaacaatcc tctcgcgctt ggtcttggtg ggagtgaaga gagcggggtt gcgactcgtg
     4741 cccatggcaa acctatagtg gaccctatga caccctctta acccggttga tctaatggtc
     4801 ccttacaact ggagaggacg gaatgccgca cggtccagac attccgccca ccacctcggt
     4861 tctgctcatc gacgagcgtg aggccgaccg cacacactgg gcagtccaac tcaagcagtg
     4921 ttcccccgac tacgagatcg ttgaagccac agatgggcaa tcaggacttg atgtctgcaa
     4981 gtctcggcgc attgattgcg tcgtgttaga aatcgagtta cctgacatat cagggttcga
     5041 agtcttgacg actctagtcc cacgtgcgaa caagccgaac atggctgtga ttgtcctcac
     5101 tcagctgatg catcgagggg tacgggaatt ggccaggaca aatggggcat atgtttgttt
     5161 tgtcaagcgg cacacgacag gcgaggattt ggatagagcg attcaacggg ctgtggcgct
     5221 ggtcggacag atgccgaagg aagatcggta tcggaacatc taatcttcgg tctgacggct
     5281 gcccccccga ctgtctagta tttcccccac gagaaatact ccccctgaga cttccctttc
     5341 atcctccccg cgacatcgtc agtgacaaga cgtttcttgg ttctttccgg ggtgttgctg
     5401 atatcggaaa gttgcgccca tgacaccttg acttggtatc gctcgtctgc tgatttcatc
     5461 gcggtgaacg tgtgcgagag cggatgattg ggcgtgtcgg tcagttcaaa ccctttgaat
     5521 tggccgatga gatagagtcg aatgacttcc aggttcgctt cgatgtgggc gttgttttcg
     5581 gtgctcatgc ggttcctctc agaaatccgg cttttcaagt tgtttgaggc ctgaaccggt
     5641 aatggcaacc atctggtcct tgccgttacc cacttcgata tgccccgcct tttccaaggc
     5701 atgcaagaca ggctgtatgt tggtctgtcg gtctatggcg aagtgaatat aaagcgtgtc
     5761 ccacttcttt gggctgtaca gcttgagatg ttccaagacc tcgcgctcaa ggcttcgctt
     5821 cctttgatat tcattttctt gctttggccc aaagtgtgcc atgacaaata ttatgcaggc
     5881 ggggccagac attcaccaca gaaaatgaag atacagtcat ttggtggtgt gctcgaccac
     5941 aaatatgagc ggaaggcaac gcgccgctga ctgtggaagg attggtgggg atgttgcgag
     6001 gctaggccgg gggattccga agcggcgctt ggatgttttc atatccctgg agccacccat
     6061 gcaccacaca ccatttgtca gcgttggatt cgaagacaag caagtccggt ctatgctttg
     6121 gcaaccacga aaaatgaaat ttcatcatat cggtgatttt atacgagtcg cattcttgcg
     6181 accaaatccg ccattgggcg atgattaaga gttccgctct cttgctggtc agtaccatga
     6241 taaaaatacc tcacgggtgg aaggagaatt ccttctcaac agtagcgaca ttcatgcggg
     6301 acaggatact cccggcaccg cgagaggcag ctaagaaggg ctggtcgagg ctcgctgata
     6361 taggtgacaa accgttagcg agcaaccgca tcaccgcagg caaggcagcg atagttgcca
     6421 gtcataaagc cgtcctcgct acgctccaaa tcgatcttga gatgttcgca gagtgggaaa
     6481 ccttgctcgt tccacgtcgc ttggaggtac gtcgcctgca ctttcgtcat atctctcccc
     6541 catggccttg ttggattgtg agggctaggc gagccagatg tatttgcact cgtgattcag
     6601 acactgccat atgagcgtga agggtcggtg agggcgtg
//
LOCUS       DATIPF010000023         3500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1683198, whole genome shotgun sequence.
ACCESSION   DATIPF010000023 DATIPF010000000
VERSION     DATIPF010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683198"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VLL06_00620"
     CDS             complement(<1..721)
                     /locus_tag="VLL06_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS29498.1"
                     /translation="MASSIFVVDSSPAVRRLVEQISKPEGCDVVGFQDGPAALEAARR
                     LSPNLIIADYHLENMTFSGFCKEVHKLDNLAETYIVSLVSPADHVDENHLRSLGVKAF
                     LNKPFQSENLIDVIKDLEAQQATKANGIKKKSRSWPPVSSSTDSDDDAINDDSLTEED
                     EEQAIAPNPQLKDSPVTTTAAKSSTAEPEEAMKGLFGQLLHSMSERTEKKIVELLPRM
                     VGKDLAALVAKSVETEVRTQLG"
     gene            915..1577
                     /locus_tag="VLL06_00625"
     CDS             915..1577
                     /locus_tag="VLL06_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HSS29499.1"
                     /translation="MNGSVADIYLKHAVQHGFRQTPDSQALIAQAFQRAFRDASPPVF
                     AATDPIKLKQSERLWWFDIVHNVFYRVGMFERFDDFFEQVFQVFEDPRSWALFPETHA
                     VLTKLREEGFELGVVSNFDSRLFPVMRGLGIERLFDTVTISSLAQAAKPASKIFEIAL
                     EKHSMDPGEAMHVGDSVRDDMEGATKAGLTGVLLDRTGRVRVSGGPVIRTLEELLPLV
                     GC"
     gene            complement(1701..2429)
                     /locus_tag="VLL06_00630"
     CDS             complement(1701..2429)
                     /locus_tag="VLL06_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HSS29500.1"
                     /translation="MATDNQILLARTHLASARNVTILTGAGISADSGVPTFRGVDGLW
                     RTFRAEDLATPEAFERDPRLVWEWYNWRRELIATKSPNPAHNTLVKLEQRCTDRTWLI
                     TQNVDGLHRAAGSQRLSEIHGNIWKVRCTGCGVISDNLEIPIPILPTCRLCHALLRPH
                     IVWFGESLREEDLRRCDEALRACDLLLVIGTSGIVYPAAGFASVAKHVGALVIEINVE
                     STPQSDLVDLSLQGRAKDLVPLLL"
     gene            2515..>3500
                     /locus_tag="VLL06_00635"
     CDS             2515..>3500
                     /locus_tag="VLL06_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HSS29501.1"
                     /translation="MKQILMVGAGSVGGFFGAQLAKANLPVSFLLRPKTLAAVKHNGL
                     TIRSAQGSFTVHPPAAADPRELPKPDLIILSVKAYDVDVVLTQLEPVMTDRTIILTLQ
                     NGVDTEDRIIARFHRDCVVGGIAYIYSKIAEPGVIDHYKRGTVAIGELMGYQSARLLA
                     IADLFKQAGIPCQLTEDVRRSKWEKMCWNCVFNPLTVLIDDKVAKALDHPEMLRVIHQ
                     IVGEVVAVAATVKVPLAADMADKVVRWTQEIRDIHTSMYDDWKAGRPTEIDFLNGYVA
                     KIGRQFGIPTPLNDMLTAVIKAVTERDRTGPGVLRIEGGVVQPVSLDRAAIAS"
BASE COUNT          724 a          889 c         1056 g          831 t
ORIGIN      
        1 tccctaactg tgtgcgcact tctgtttcta cggatttggc tacgagagct gccaggtctt
       61 ttcccaccat ccgggggagc agctcaacga tctttttttc cgtccgctcc gacatggagt
      121 ggaggagctg tccgaagagg cctttcatcg cctcctcggg ttcggcagtc gatgatttgg
      181 cagctgtggt ggtgacaggg gagtctttca gttgggggtt gggggcgatc gcctgttctt
      241 catcttcctc ggtcaggctg tcatcgttaa tggcatcatc atcggagtct gtcgagcttg
      301 aaaccggagg ccaggatcga gatttcttct tgattccatt tgccttggtt gcctgctgtg
      361 cttccaagtc cttaatcaca tcaatgaggt tttcggactg aaagggtttg ttgagaaagg
      421 ctttcactcc cagcgaacgt aagtggtttt catccacatg gtccgcaggg ctgactaggg
      481 agacaatgta ggtttctgcc agattatcca gcttgtggac ttctttgcag aaccccgaga
      541 aggtcatatt ctccaaatga tagtctgcga tgatgagatt cgggctcaat cggcgtgcgg
      601 cttccaaggc tgcagggcca tcctggaatc ccacgacgtc acacccctcc ggtttcgaga
      661 tctgctcgac cagccggcgg acggcggggc tactatcgac gacaaaaatg ctcgaagcca
      721 cctaaccctc caatgttatt gaactatgtc gaagctagca gtgggggtat tctttgtcaa
      781 gaaaccgcac gatcggtgca cgagttgact cgggtttttg aactgatgac aattccaatg
      841 cagcaggatt tgtagaacct atgaagaccc ctattcacgt catctttttt gatgcagccg
      901 agacgtagtt tcatattaat ggctccgtgg cggacatcta cctgaagcat gcagttcagc
      961 acgggtttcg gcagactccc gactcgcagg cattgatcgc acaagccttt caacgtgcgt
     1021 ttcgcgatgc gtcgcccccg gtttttgccg caacggatcc gatcaagttg aagcaaagcg
     1081 agagactctg gtggttcgac attgtgcaca acgtgtttta tcgcgtcggg atgttcgagc
     1141 gcttcgatga ctttttcgag caggtctttc aggtgtttga agatcctcgc tcctgggcgt
     1201 tgtttcctga aacgcatgct gtgctgacca agctgcggga agaggggttc gagcttgggg
     1261 tcgtgtccaa ctttgactca cgtctctttc ctgtgatgcg ggggctcggg atcgagagac
     1321 tcttcgatac ggtgacgatt tctagtctgg cacaggcggc taagccggcg tcaaagattt
     1381 tcgagattgc ccttgagaaa cattcgatgg atccgggcga agcgatgcat gtgggtgaca
     1441 gtgtccgcga cgatatggag ggtgcgacga aggccggcct gaccggcgtg ttgctggatc
     1501 gaacagggcg cgttcgggtg tcgggtgggc ctgtcatccg gacgttggag gaactgctac
     1561 cgctcgtagg atgctaagag accgttattt ggtttgtctg gtttgtttag tttatctggt
     1621 gttttggttg aacgaaagaa accaaacgag cgaaattaac cagatagacc agacaaacca
     1681 gatgaaccag atagaccggg ttataggagc agcggcacca ggtctttcgc acgaccttgc
     1741 agcgagaggt caaccaggtc tgactgcggg gtactctcga cattgatctc aatgaccagg
     1801 gcgccgacgt gcttcgcgac cgacgcaaat cccgcagcgg gatagacgat tcccgatgtc
     1861 ccgatgacaa ggaggaggtc gcaggcccgg agggcctcgt cacagcggcg gaggtcttcc
     1921 tcccggagcg attccccaaa ccagacgata tgtggccgga gcagtgcatg acacaaccgg
     1981 catgtcggca ggatgggaat ggggatctcc agattgtcag agatgacgcc gcagcccgta
     2041 cagcgaacct tccagatatt gccgtgaatc tcagagagcc gttgtgatcc ggcggcacgg
     2101 tggagtccgt caacattttg cgtgatgagc cacgtgcgat cggtgcatcg ctgttccagc
     2161 ttcacaagcg tgttgtgggc gggattcggc gatttcgtgg caatcaattc acgccgccag
     2221 ttgtaccatt cccagaccag ccgtgggtcc cgttcaaagg cctcaggagt tgcgagatcc
     2281 tccgcgcgaa aggtgcgcca caagccgtct actccgcgaa aggtcggcac gccgctgtcg
     2341 gcagaaatgc cggctcccgt gaggatcgtc acgttccgag cggaggcgag atgggtgcga
     2401 gcgaggagaa tctgattgtc agtggccatg atcgaatgtg cggattgtac cgcatttcga
     2461 ccccatgaat ctgcgtggta tagtaccgcc tcctctattt tcatgaggtg aaaaatgaaa
     2521 cagattctta tggtgggggc tggctcggtc ggtgggttct tcggtgcaca gttggcgaaa
     2581 gccaatctcc ccgtgtcttt cctgctgcgg cccaagacgc tcgcagccgt gaagcacaat
     2641 ggcctcacga tccgcagtgc ccagggctcc ttcaccgtgc atccaccagc tgccgccgat
     2701 ccgcgagagc ttccgaagcc ggacctgatt attctctccg tcaaagccta cgatgtggat
     2761 gtggtgctga ctcaactcga gccggtcatg accgatagga ccatcatctt gaccctgcag
     2821 aacggggtcg ataccgaaga ccgtattatc gcgcgctttc atcgggattg tgtcgtgggt
     2881 ggtattgcat atatttactc caagatcgct gaaccaggtg tcatcgatca ctataaaaga
     2941 ggcactgtgg cgatcgggga gctaatgggg tatcagagcg ctcgtctgtt ggccatcgcg
     3001 gatctgttca agcaggccgg gattccttgt cagctgaccg aggatgtccg gcgcagcaag
     3061 tgggagaaga tgtgttggaa ttgcgtgttc aatcccttaa ccgtcttgat cgacgacaaa
     3121 gtggccaagg cgctggacca tcctgagatg ttgcgtgtca ttcatcagat cgtgggtgaa
     3181 gtcgtggcgg tggcggctac ggtgaaggtg ccgttagccg ctgatatggc ggacaaggtg
     3241 gtgcgttgga ctcaagagat tcgggatatc catacctcga tgtatgacga ttggaaggcg
     3301 ggccggccga ccgagatcga ctttttgaat ggatatgtcg caaagatcgg gcggcaattc
     3361 ggcattccaa cgccactgaa cgacatgctg acggcggtga tcaaggccgt cacggagcgg
     3421 gatcgaaccg gtccaggggt tttgcggatc gagggggggg tggttcagcc tgtctcgctt
     3481 gatcgtgccg cgatcgcatc
//
LOCUS       DATIPF010000024         3988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1595072, whole genome shotgun sequence.
ACCESSION   DATIPF010000024 DATIPF010000000
VERSION     DATIPF010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595072"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..330)
                     /locus_tag="VLL06_00640"
     CDS             complement(103..330)
                     /locus_tag="VLL06_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29502.1"
                     /translation="MSLSFHGAVQCVTVSRHMMIETPVFRLLLDCGLFQGPRREEALH
                     RNCELGFDQKLIGTLLLSHAHIPHVGSSYDV"
     gene            complement(330..1193)
                     /locus_tag="VLL06_00645"
     CDS             complement(330..1193)
                     /locus_tag="VLL06_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HSS29503.1"
                     /translation="MRAMEPESSDSASHHLQPDVTSPSYIPAFLDTEFLQREELRALR
                     IGLELLKPELIQREEGVRSTIVVFGSARLLESVAAAHALHIAEEEATRAPNDPMHRQR
                     VAIAQRQLALSKYYEVAREFSRLVSSTCQIDGHCDYVIVTGGGPGIMEAANRGAADVG
                     AKSIGLNITLPHEQYPNPYITPRLSFQFRYFAIRKMHFLIRAKALVAFPGGFGTLDEL
                     FETLTLLQTGKTENVMVVLVGRDFWDRLINWQWLVENGFIAQQDLRLFHYAETAQEAW
                     DLIARHNGVSS"
     gene            complement(1393..1830)
                     /locus_tag="VLL06_00650"
     CDS             complement(1393..1830)
                     /locus_tag="VLL06_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29504.1"
                     /translation="MSDRRRGLMILVCLLAVLLEGCPLAWRRVTVNEIIRQDDVSFIV
                     PGATTLHEIVKNLGAPGSIRRVDSGTVVRYQFLDIKHFTVNITRPLAFFFPFLEVVPS
                     DLYQLTYGGGGLGTEELQVEFDKNWVVVYYAFAHHQEASRYTP"
     gene            complement(1837..2211)
                     /gene="bamE"
                     /locus_tag="VLL06_00655"
     CDS             complement(1837..2211)
                     /gene="bamE"
                     /locus_tag="VLL06_00655"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HSS29505.1"
                     /translation="MLVVRSLLVVVAAATLVQGCAFFRGNYDDDFKQEDVESITIGAS
                     TRQDVANILGAPERVIEVHDKEIFHYYNYTVKSGTVLFFSRTNVVGNDLYIFFSPDGI
                     VQQVVMGRQKPPPKLQFWPFGD"
     gene            complement(2237..2656)
                     /locus_tag="VLL06_00660"
     CDS             complement(2237..2656)
                     /locus_tag="VLL06_00660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSS29506.1"
                     /translation="MAQETEGRCLDLDRLLLGTIALIETPARGFFMVAELEQADDRPL
                     LGQLMVTYEWSDWRNGAFWWIQSVYVDPAWRRKGIYRRLHDTVVEMGKADNEMCGIRL
                     YVARDNRAAQTVYRRVGLTPSMYDVYENDFVLKRHGS"
     gene            complement(2740..3354)
                     /locus_tag="VLL06_00665"
     CDS             complement(2740..3354)
                     /locus_tag="VLL06_00665"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HSS29507.1"
                     /translation="MSKTLWKYSLAGLLVLVPAWATFLILSTLFSALNELIHDLPWNI
                     GAGQRPGLSLVLLLVLVVAIGAIATHVIGQRVITRTERWIEHVPLVRSVYGTLKGMAD
                     LLNFRSRFGRSTVVAFPFPRDGLWAIGFVMGTAPSALQVAPTPLLMVFVPTAIHPFTG
                     FLALVPQPQLRPINLPPEDAIKMECTAGLYKPETGWLQTPKTTV"
     gene            3508..3798
                     /locus_tag="VLL06_00670"
     CDS             3508..3798
                     /locus_tag="VLL06_00670"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HSS29508.1"
                     /translation="MADRTESRRPVNINKDLLAILCCPDTKQDVSLADEALIAKLNEA
                     VSRGKLKNKANKPITEPLDGGLIRGDRKMLYPIREDIPVMLIEEGIPLEGMI"
BASE COUNT          965 a         1053 c         1079 g          891 t
ORIGIN      
        1 aatcgggaat cttccgactc actggcttgg gcggacaggc acgaggttgt gcagatacca
       61 acggccagga tgagcagcgt aattgtcggc cggacagtgc cgttagacat cataactcga
      121 tccgacatga ggaatatggg catgggatag caacagggtt ccgatcagtt tttgatcgaa
      181 gcccaattcg caattccgat gaagtgcctc ttctcgacgt ggtccttgaa agagcccaca
      241 atccagcagc agtcgaaata ctggtgtttc aatcatcata tggcgactta cggtgacgca
      301 ctgtaccgca ccgtggaagg agaggctcat tatgaagaca ctccattatg gcgtgcgatc
      361 aaatcccagg cttcttgagc cgtttcggcg tagtgaaaca ggcggaggtc ctgctgagca
      421 atgaacccat tctcgacgag ccattgccag ttgatcaggc ggtcccaaaa gtcccgtccg
      481 accagcacga ccatcacatt ttctgtctta cctgtttgga gaagcgtcaa cgtctcgaat
      541 agctcatcga gagtaccgaa tcctccagga aaggcgacca gggccttggc acggattaag
      601 aagtgcattt tcctgatggc gaaatagcga aactgaaagc tgagtctcgg cgtgatgtag
      661 gggttcggat actgttcgtg cggtaaggta atattcagtc cgatcgactt tgctccaacg
      721 tctgctgctc cacggttcgc cgcttccata atgccgggcc ctccgcccgt gacaatcacg
      781 taatcgcaat gaccgtcgat ctgacaggtc gaagaaacca atcggctgaa ctcacgcgcc
      841 acctcatagt atttcgagag cgcgagttgt cgttgcgcga tcgccaccct ttgccgatgc
      901 atgggatcgt ttggggctcg agtcgcctct tcttcagcta tgtggagcgc atgcgccgca
      961 gcgaccgatt ctagtagcct ggcactgccg aatacgacga tggtcgaccg cactccctcc
     1021 tcacgctgga tcaactcggg cttcagaagt tcgaggccga tgcggagggc gcggagttct
     1081 tcgcgctgga gaaattctgt gtcgagaaag gcagggatgt aggatgggga cgtgacgtcg
     1141 ggctgaaggt ggtgggatgc actatcggat gattccggct ccatggcccg cattatagcg
     1201 acctgcacct actgcggcaa ttgcaggaca ttcccgcgag agtggcacag gcggcctgat
     1261 ggaaacctgt caatgtccga ttaacgccat ggcaatgtcc gcgatttacg aacatgcgat
     1321 gtggaactat tccgaagttt gcacagttgc tgtgacaaga tcatcgaagg agaatcgaaa
     1381 tgtcagttga gcctacggcg tataacggct cgcttcttga tgatgggcaa aggcgtaata
     1441 caccacgacc cagttcttat cgaattccac ctgcagttcc tcggtgccta atcccccacc
     1501 gccataggtg agttgataaa ggtcgctggg gacaacttcc agaaatggaa aaaagaaagc
     1561 cagcggacgt gtgatgttca ccgtaaagtg tttgatatca agaaactgat accgtacgac
     1621 tgtgccgctg tccacccgcc ggatactccc tggggctccc aggttcttga cgatttcgtg
     1681 gagtgtcgtt gccccaggca cgatgaaaga cacgtcgtcc tgacgaataa tttcattgac
     1741 tgtgacgcga cgccaagcca gagggcatcc ttctaaaagg acggccagaa ggcacacaag
     1801 aatcatcagt ccccgtcgcc gatccgacat cgtcgattag tccccaaatg gccagaattg
     1861 tagtttggga ggcggtttct ggcggcccat cacgacttgc tgtacgatgc cgtcgggaga
     1921 gaaaaatatg tacaggtcgt ttccgacgac attggttcga gagaaaaaca ggacggtgcc
     1981 ggatttgacg gtgtaattgt agtagtggaa tatctcctta tcatgaactt caatgacacg
     2041 ctccggtgca ccaagtatat tcgcgacgtc ttgtctggta gaagctccga tcgtaatcga
     2101 ctctacatcc tcctgtttga aatcatcatc ataattgccc cggaaaaacg cgcatccctg
     2161 tacgagggtg gcagcagcca caacgaccaa caacgaccga accacaagca tggagcctcc
     2221 tttccttctg cccgtcttac gagccatgac gcttcagaac gaaatcgttt tcgtagacgt
     2281 catacattga tggtgtcaga ccaactcgcc ggtacacggt ttgggccgct cgattgtctc
     2341 gcgccacgta cagtctaata ccgcacatct cgttatcggc tttgcccatc tcaacgacgg
     2401 tatcatgcaa ccgccgatag attcctttcc gtctccaggc gggatccaca tacacactct
     2461 gaatccacca aaaggcaccg ttacgccaat cgctccattc ataggtgacc atcagctgtc
     2521 cgagcagtgg gcgatcatca gcctgttcca gttcggcaac catgaaaaat ccccgcgcgg
     2581 gagtttcgat cagggcgatg gttccgagaa ggaggcggtc gagatcgagg cagcggcctt
     2641 ccgtttcttg agccattgct gcactgaacg acgcgatcgt ctctacatct tccggcctgg
     2701 cacgtctaat attcaggtga tcaaggcctg acatgtgtct tagaccgttg tcttcggggt
     2761 ttgtaaccat ccggtctccg gcttgtacaa gccggcggta cactccatct ttatggcgtc
     2821 ttccggtggc agattgattg ggcgtagctg gggctgcgga accaacgcga ggaagccggt
     2881 gaagggatgg atggccgtgg gcacaaagac catgagcaat ggtgttggtg cgacctgcaa
     2941 cgcagacggt gcggttccca tgacaaagcc gatcgcccag agtccgtctc gaggaaacgg
     3001 aaaagccacc acagtgctcc gcccgaatcg ggaccggaaa ttaagaagat cggccatccc
     3061 tttgagcgtg ccgtagacac tgcggacgag tggaacgtgc tcgatccacc tttcggttct
     3121 ggtgatcaca cgctgtccta tgacatgggt ggcaatggca ccgatggcca caaccaggac
     3181 caataacaag accagactga gtccaggcct ttgtccggcc ccgatattcc acgggagatc
     3241 atgaatgagt tcattgagtg ctgagaatag ggtggaaagg atcaagaatg tggcccaggc
     3301 cggcacgagg accagcaatc cggccaaaga gtacttccat agtgtctttg acatcgcggc
     3361 ttgtttcctt ctctacgatc aggcgaagcc ggaactctag cagaaacggc cacccaaaat
     3421 ggagtctgta cgctgggctt gatctcttcg agttcggtga tctattgtac aaataaggga
     3481 ccgtttgatc cacacaaggg ggatattatg gcagatcgta cggaatccag gcgtccagtc
     3541 aatatcaata aagatcttct cgccattctc tgttgtcccg acacaaagca ggacgtcagt
     3601 ttggctgatg aggcactgat tgccaaattg aatgaggccg tgagccgtgg aaaattgaaa
     3661 aataaagcga ataagccgat cactgaaccc cttgacggag ggttgatccg gggtgatcga
     3721 aagatgctgt atccgatccg cgaagacatt cctgtgatgc ttatcgaaga aggcatccct
     3781 cttgaaggga tgatctagca agtcttactc accaaccgat gtcttctgca agcaggccac
     3841 actatctctt catcccgaaa attctgttcc ctgcgcaatg agagaagact cgaataggcg
     3901 cgatcgttgt tgaggcgcgg cagccggcga gttacctatt gattgtcggt cttagacccg
     3961 gtggacgttc cacagtgccg acaaagat
//
LOCUS       DATIPF010000025         3342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_799555, whole genome shotgun sequence.
ACCESSION   DATIPF010000025 DATIPF010000000
VERSION     DATIPF010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799555"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VLL06_00675"
     CDS             <1..665
                     /locus_tag="VLL06_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSS29509.1"
                     /translation="VATDLEAAFETLQLPPGFSIRLAGQIQQQRETFEGLLFATVLAL
                     ILVYMVMAAQFKSLIDPFVIMFSVPMGFPGVILILFLTDTTLSTTSMMGIIMMLGIVV
                     SNGVLLVDYTNVLRRRGRELHDAAVSAARTRLRPILMTSLATVLGLLPMAIGWGTGGE
                     TNAPLARAVVGGLSVSTILTLFLVPTVYVILEERFPRDAGRTAEEADWIPAEPGQAPL
                     PR"
     gene            complement(725..1057)
                     /locus_tag="VLL06_00680"
     CDS             complement(725..1057)
                     /locus_tag="VLL06_00680"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HSS29510.1"
                     /translation="MDPTTVKEFFQHLPGLLDSGAAEDLDAVYQFDLSGAQGGQYILT
                     IRDGACQVAEGMHADPHVVLSMAGEDCIKILKGQLSGKAVAMTGRLKVSGDLGLAMQL
                     KALFPTVR"
     gene            complement(1129..1569)
                     /locus_tag="VLL06_00685"
     CDS             complement(1129..1569)
                     /locus_tag="VLL06_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HSS29511.1"
                     /translation="MSSSTDSESAIFPTESPVAASFASQEMVGEGKAWGLCTAVDLQD
                     CHPDLIRNADHIRRYVVELCELIDMKRFGECQVVDFGSGRVAGYSMVQLISTSLISGH
                     FANDTNNAYLDIFSCKGYDPAIVEAFSKDFFGARRSTATVTLRY"
     gene            complement(2254..2577)
                     /locus_tag="VLL06_00690"
     CDS             complement(2254..2577)
                     /locus_tag="VLL06_00690"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS29512.1"
                     /translation="MGFKRKGSRIEVSRAGRLQRGSLSAPCRILDVSETGVRIESRLF
                     VKSGDALQLVIELEKGRMLTCGLQVIHVRSPKFGTKITSISPEDRERLSHILDDHVQT
                     SFSRR"
     gene            complement(3000..>3342)
                     /locus_tag="VLL06_00695"
     CDS             complement(3000..>3342)
                     /locus_tag="VLL06_00695"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSS29513.1"
                     /translation="AVRWPSEISQQGTPLGLTPLNDVQAQRLPKVMVRVEAGITFVDH
                     TIVLGRRQAIAGVEHALMGMKAGGYRKVRMSPHLAYRDAGVPELIPSNAVLVVEIWLR
                     AIDQVRTVPAS"
BASE COUNT          717 a         1003 c          943 g          679 t
ORIGIN      
        1 ccgtcgccac cgacctggag gcagcctttg agacgctcca actcccccct ggcttcagta
       61 tccggttagc cggccagatt caacaacagc gagaaacgtt cgagggcctg ctgtttgcga
      121 cggtcctggc gctgattctc gtgtacatgg tgatggccgc ccagttcaaa tcgctgatcg
      181 atcccttcgt catcatgttt tcggtcccga tgggcttccc tggcgtgatc ctgatcctct
      241 tcctgaccga caccaccctt tccaccacgt cgatgatggg tatcatcatg atgctgggca
      301 tcgtggtctc aaacggcgtc ctcttggtcg actatacgaa tgtgctacga cggcgaggac
      361 gggagttaca cgacgcggca gtgagcgcgg cgcggacacg gctccggccc attctcatga
      421 cctcgctcgc gaccgtcctg gggctactcc ccatggccat cggttggggc acaggcggtg
      481 aaaccaacgc cccgttggcg cgcgcggtcg tcggaggact cagcgtctcg acgatcctca
      541 ccctctttct cgtccccacg gtctacgtga tcttggagga acggttcccc cgcgatgcag
      601 gcagaaccgc agaggaggcc gactggatac cggcagagcc tggacaggca ccgcttcctc
      661 gatagcagga agctgaaaac atccctcttc cgtgtttctg cagtttgagg aaagagctgg
      721 tgggttacct gacggttgga aagagcgcct tcagctgcat cgccaagcca aggtctccgc
      781 tgaccttgag ccggcccgtc atggcaacgg cttttccgct cagctgaccc ttgagaatct
      841 tgatgcaatc ctctcccgcc atcgagagca cgacgtgcgg atccgcatgc atgccctctg
      901 cgacctgaca ggccccgtcc cggatcgtga gaatatactg tccgccctgc gcaccgctca
      961 gatcgaattg gtagacggca tcaagatctt cggcggcccc tgaatcaagc agaccaggaa
     1021 gatgctgaaa gaactctttg acagtggtgg gatccatggt tcgtcggaag ggaagtgcct
     1081 cttggaccga taccgggagc cgcgctgccg cagcccccgg tcctcagcct agtaacggag
     1141 tgtgaccgtc gcggtactgc gacgcgcgcc gaaaaagtct ttggagaatg cctcgacgat
     1201 ggcagggtca tagcccttac agctaaaaat gtcgaggtag gcattgttcg tatcatttgc
     1261 aaaatggccg ctgatcaacg aggtcgagat gagttggacc atggagtagc cggcgacgcg
     1321 gcctgagccg aaatcgacaa cctggcactc gccaaaacgc ttcatgtcga tcagctcaca
     1381 cagctcgacg acatagcgac ggatatggtc ggcattgcgg atgaggtcgg gatgacaatc
     1441 ttggaggtcg acggcggtac agagtcccca cgccttccct tcacccacca tctcctggga
     1501 ggcaaaggaa gcagctactg gggactcggt tggaaagatt gcggactcgg aatcagtcga
     1561 gctactcata aagattgtac acctttctgt taagagggtg aaaaaactgt gacgatgaac
     1621 tgttcagtcg gactatacca cgaaactttt cacatcaggg gggatggaaa aacttttttt
     1681 cacaccgaga ggtttgagga ccactcgagc gattgacaag gttctcagcg gtcggagaga
     1741 atgccgccca tgaccaatgt cgccccgtct gaaccgaacc cgtctccatc ctccgccgac
     1801 ctccccgatc gtctctgcac ctggtgtcgg ctgccgatga agaagcgcct cgtcggcggc
     1861 aagcagttca tccactatac ttgcccaaag tgcgtcttcc agcacacaac caggctcggc
     1921 ccaaaacctc ccagcaccgc ccactgacag gctgggaaaa atcttttgtg gcagactaac
     1981 gcctcgacgg tccgtacgtg tgcttgcgac gagctcagtc gagccgcatc acaggaaaac
     2041 attcccgcag gatgctcaaa aaggccgtcc tgcgaggccg caggcgagtc gaaaccggag
     2101 acgtaccctc tggggcgcac ggtgcgacga ataaggagca ccacgtccgt gcgcgccgcc
     2161 gagtcggtga ggtggccggg tcccccttga ggatttcgat gagccgagaa cgaagctggc
     2221 gggctttttc agcatcctgc tagcgcggag cggctaccgc cgtgaaaaac tggtctgcac
     2281 gtgatcgtca aggatatggc tcaatcgctc tctgtcctcg ggactgattg aggtgatctt
     2341 cgtgccaaac ttcggggaac gcacgtggat gacttgcaag ccacatgtca gcattctccc
     2401 cttctctagc tcgatgacaa gttggagtgc atcgccgctc ttcacgaaca agcggctctc
     2461 aatccggaca cctgtctcgc tgacatccag aatcctgcat ggcgctgaga gcgacccccg
     2521 ctgcagacgg ccggcccggc tgacttcaat gcgtgatccc ttccgcttaa atcccatgcg
     2581 ctcctcctat tcgggggatc ataccaattg accgaccctc tcaaaagaaa aatgctgaaa
     2641 gaagctacaa ctgttctccg acaaagctgc gcacctgctc cattcgacta caattgacgc
     2701 ccagatcgcc gtagccaggg cgggaaacgg aacggaagtg gattgtcttg gattcgtcgt
     2761 cgaagacaca ctcgacgtca taaccgaagc atgaggctgg tgaactcata atgcagatag
     2821 gactcgtctt cctcaacgcg cttcgtgcga ggcagcgagc gtacaatctc ttttaatgcc
     2881 tcctttgcct cggctcgtga tttgcgataa cgtataggcc cgatcgcatg cccttcgtcc
     2941 tgagcttggg tcgagacaca gttggggctg gaggggtacg gggcgagtgg tgcggatgct
     3001 catgatgcag gaactgtacg cacttgatcg atggctcgca accagatctc aacgaccaac
     3061 accgcattgg agggaatcaa ttcgggaaca ccagcgtcgc gataggctag atgcgggctc
     3121 atgcggactt tgcgatagcc gccggccttc atgcccatca aggcatgctc caccccggca
     3181 atggcctggc gtcgcccaag cacgatggtg tgatcgacga atgtgattcc tgcctcgact
     3241 cgcaccatga ctttgggaag ccgctgtgct tggacatcat taagcggcgt gagcccgagc
     3301 ggcgtgccct gctgcgatat ctccgacggc caccgcacgg ca
//
LOCUS       DATIPF010000026         3752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_357402, whole genome shotgun sequence.
ACCESSION   DATIPF010000026 DATIPF010000000
VERSION     DATIPF010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357402"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VLL06_00700"
     CDS             <1..922
                     /locus_tag="VLL06_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS29514.1"
                     /translation="FHFLDPFASHVDFDKIVVAVQQPEACALPDMAMTASDIGGRILV
                     AEDNPVNHEVALAMLESLGCRVDGVCTGQEAVEAASRVPYDLILMDCQMPGMDGYAAT
                     RSIRRNERQGARRIPIIALTAHASKGDRAMCLAAGMDDYLSKPYTHQKLQAIVQRWLS
                     MSLSTAPSLSEGLRQGTSAPSVPPYSGGPVILDSRAVDAIRALQRNGRPDFLARLIEK
                     YVTASTEHMVSIRRAVASGDAMALWQAAHALKSSSGMLGASIFAELCHELEMLGRAET
                     LERVPEVLRKLEVSYPSVCAALEGEAGKSQ"
     gene            924..3065
                     /locus_tag="VLL06_00705"
     CDS             924..3065
                     /locus_tag="VLL06_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSS29515.1"
                     /translation="MESQVERTASRVLVVDDDAVVRMLVSEALRQSGLEVCEAESGAQ
                     ALVLFAASRPDLVVLDVMMPGLDGFAICTKLRGCIGGSRVPILIMTGLDDPESIARAY
                     EQGATDFITKPINPMILSNRVRYMLRGSHTLDALLRSEARLGFAQRIAKIGNWEWRPD
                     TNQFTASSELCRLVGVRPEDFGGTFDAFLHLVHPEDRDCVEEALKGILTRRVTCNIDH
                     RLVLPNGMDVTVNLQAEAVLDDQMRSLSILGTAQDITDRKQSEQEIHRLAYFDSLTGL
                     PNRILFKDRLTQALVHAHRYRTTLATLFLDLDRFKVINDTLGHNIGDLVLKSVAERLA
                     ESVRHSDSVCRSAGELENHSLARLGGDEFTVLLTNLREVQDAGKVARRIVESLAQSFS
                     IEGREIFVTVSVGIAIFPDDGDSVDALLKNADSAMYHAKEHGRNNAQFYSNTFNAAAH
                     ERLNLEGELRHALVREEFVVYYQPQIDLQTNKVVGAEALVRWQHPQRGLLPPAVFLPA
                     AIDTGLIRAIDEWVLRTACRHNQAWQLRGRMPVHISVNLSHSLFHSKTLLSVVEEALS
                     QTGLIPACLKLELTESIAMRNVDASIVMLTTLKAMGVQLSIDDFGTGYSSLSYLQRLP
                     INMVKIDQSFIQEILTQASPSPIVRAIIAMAHSLNLLVLAEGVEQEAQRMLLLEEGCD
                     QAQGYLFGRPMPEEAFATLLPLFHLHIQKAG"
     gene            complement(3341..>3752)
                     /locus_tag="VLL06_00710"
     CDS             complement(3341..>3752)
                     /locus_tag="VLL06_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29516.1"
                     /translation="QVYETGSITFDGVGQAISTHKGLSIASVAPAFNTPPYAVTYDQI
                     CRFTYVMKNLSSFTLHNGSCSGTNTTGPTAFGVPGSTAKLTGLTYEGQIGALGAVLTY
                     GATMPSQSSLEVSNGFNATQLCGTVVTAVRTIHG"
BASE COUNT          784 a          981 c         1134 g          853 t
ORIGIN      
        1 ctttcatttt ctcgatccat ttgcgagtca cgtcgacttt gacaagattg tcgttgcagt
       61 acaacaacca gaagcctgtg ctcttccaga catggcgatg actgcgtcgg atattggtgg
      121 tcggatcttg gtggccgaag acaatccagt caaccacgag gttgcgctcg ccatgctgga
      181 gtcgttgggg tgtcgagtgg atggtgtgtg cactgggcag gaggccgttg aggccgcttc
      241 gcgagtcccc tatgacctga ttctcatgga ttgccaaatg cccgggatgg atggctatgc
      301 ggcgacccgc tcgatccgcc gcaacgagcg acagggtgcg agacgcattc cgattattgc
      361 ccttacggct catgcctcga agggagatcg ggcgatgtgt cttgcggctg ggatggacga
      421 ctacctcagt aaaccttata cgcaccagaa gcttcaagcc atcgtacagc gatggctgtc
      481 gatgtcgctc tccacggctc cttccttgtc cgaagggctt cgacaaggaa catctgcccc
      541 gtccgttccc ccgtattccg gagggcctgt aatcctggat tctcgcgcag tcgacgccat
      601 tcgggcgctc cagcgcaatg gccggccgga cttccttgcc cgattgattg agaagtatgt
      661 cacagcgtca acggagcaca tggtgtcgat ccgacgtgca gtagcatccg gagacgccat
      721 ggcgctctgg caagcggccc atgcattgaa gtcaagcagt ggcatgttgg gggcgtctat
      781 cttcgcggag ttgtgtcatg aactggagat gttaggacgg gctgagaccc tcgagcgggt
      841 ccctgaagtg ctgcgcaagc tggaagtatc ttatccgagc gtatgcgcgg ctctggaagg
      901 agaagcgggc aaatcgcagt gatatggaat ctcaagtaga acgaacggca tcgcgggtgc
      961 ttgtcgtcga tgacgacgca gttgtcagaa tgcttgtgag cgaggcactc aggcagtctg
     1021 ggcttgaggt ctgcgaggcg gagagcgggg cacaggcgtt ggtgctattt gcggcctcaa
     1081 gaccggatct cgtggtccta gatgtcatga tgccagggct cgatgggttt gccatctgta
     1141 cgaagttgcg tgggtgcatc ggaggcagcc gcgtgcccat tctgatcatg acgggactcg
     1201 acgatccaga gtcgatcgcc cgtgcctatg aacagggcgc aacggatttt atcacgaagc
     1261 cgattaatcc gatgatcttg agtaatcgtg tgcgatatat gctgcgcggg agccacacct
     1321 tggatgcgtt gctcagaagc gaggcgaggc taggctttgc gcaacgaatc gccaaaattg
     1381 gcaattggga atggcgtccg gacacgaatc agtttacggc gtcatctgag ctatgtcgcc
     1441 tggtgggtgt tcgaccggag gattttggtg gaacatttga tgcgtttctc catctcgtgc
     1501 atccggaaga tcgggactgt gtggaggagg cactgaaggg tattttgacc aggcgagtca
     1561 cgtgcaatat cgaccatcga cttgttctgc cgaacggcat ggatgtgacg gtcaatctcc
     1621 aggcggaggc cgtgttagat gaccaaatga gatcgctgtc catcctcggg acggctcaag
     1681 acatcaccga tcggaaacag tctgagcaag agattcatcg tctggcgtat ttcgacagcc
     1741 tgacgggact gccgaaccgg atattgttca aagatcggct gacgcaggcc ctcgtgcatg
     1801 cccaccgcta ccgcaccacg ctggccaccc tttttctgga cttggatcgc tttaaggtta
     1861 ttaacgatac cttgggccac aatatcggcg acttggtgct gaagagtgtg gccgagcgtc
     1921 tagctgaatc cgtgcggcac agtgattccg tctgccggtc tgcgggagag ctggagaatc
     1981 attcactggc acgattgggc ggcgatgagt ttacggtact gctcacgaat ttgcgtgagg
     2041 tgcaggatgc cgggaaagtg gcgcgccgaa ttgtggagtc gctcgcgcaa tccttctcga
     2101 tcgaagggcg agaaatcttc gtcacggtca gtgtggggat cgcgatcttt ccggatgatg
     2161 gagactcggt cgatgccttg ctgaagaatg cggacagcgc catgtatcac gccaaggaac
     2221 atggccggaa taatgctcag ttctattcca acacctttaa tgccgccgcg catgagcggc
     2281 tgaacttgga aggagaactc cgccatgccc tcgtacgaga agagttcgtg gtgtattacc
     2341 agccacaaat cgatctgcag acaaacaagg ttgtcggtgc ggaggccctg gtgcggtggc
     2401 agcatcccca gcggggactc ttgcctccag cggtgtttct gcccgccgcg atcgatacag
     2461 gtctgattag agcgatcgat gagtgggtgc tgcgaacagc gtgtcgacac aatcaggcat
     2521 ggcagctgcg cggcaggatg ccggtgcata tttctgtcaa tctctctcac tcgttattcc
     2581 acagcaagac attgctgtct gtcgtggaag aagccctcag tcagacgggg ttgattccgg
     2641 cttgcctgaa gttagaactg acagaatcga ttgccatgcg caatgttgac gcgtcaatcg
     2701 tcatgttgac cacgttaaag gcaatggggg tgcagctctc tatcgacgat tttggcaccg
     2761 gctattcctc gctcagctat ctgcagcgtt tgcctattaa catggtgaag atcgatcaat
     2821 cctttatcca ggaaatcctg acgcaggcca gtccctcccc gattgtgcgg gcgattattg
     2881 ccatggctca cagcctcaat ctcctggtgc tcgccgaagg ggtcgagcag gaggcccaac
     2941 ggatgctgtt actcgaagag ggatgtgatc aggctcaagg ctatctcttc ggtcggccga
     3001 tgccggagga ggcctttgcc acgttgcttc ccctgtttca tctacatata caaaaagccg
     3061 gttgatttca ggatgaacct cggggtccta aaatgatcca ggttcaccta cggtctcgaa
     3121 gacgctgtgc gttcaccaac agtccaattt gtcgttgatg cccaacgcga tggataactg
     3181 gaaacaaaga attgactcgc tggacaacag ccggaaacgc ttgtgggctg atccacagct
     3241 cgtgcggccg tgggtctgcc tgcaaggcgg agcggatgcg gcgctcgccc tccagagcga
     3301 gcaccgggtt cggcaagtcg agggccactg accggctcgc ttatccgtga attgtgcgca
     3361 ccgctgtgac cacggtaccg catagttgcg tggcattaaa tccattgctc acttccagtg
     3421 atgattgcga cggcatggtc gcgccatagg tcaggaccgc acctaaggca ccgatctggc
     3481 cctcatacgt gaggccggtt agtttcgccg tgctacccgg aactccgaac gcggtcgggc
     3541 cggtcgtgtt cgtgcccgag cagcttccat tgtgtagggt gaagctcgag aggttcttca
     3601 tcacataggt aaacctacaa atctgatcgt atgtcacagc ataaggtggt gtattgaacg
     3661 cgggtgcgac ggacgcgatg ctcagcccct tatgcgttga tatggcctga cccacgccat
     3721 cgaaggtaat ggagccagtc tcgtaaactt gg
//
LOCUS       DATIPF010000027         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_888093, whole genome shotgun sequence.
ACCESSION   DATIPF010000027 DATIPF010000000
VERSION     DATIPF010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888093"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..591
                     /locus_tag="VLL06_00715"
     CDS             127..591
                     /locus_tag="VLL06_00715"
                     /inference="COORDINATES: protein motif:HMM:NF025808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiubiquitin domain-containing protein"
                     /protein_id="HSS29517.1"
                     /translation="MEIQADRTGHQVRVHIDQHSYESATPTTGEALYQLAHIRPELLL
                     YKEVGGDREDQLVSRDSQEVHLHEDEHFHSAPARHDVTIFVNTEPFPWDRPKITYEDV
                     VKLAFPNGPFGGDVRYSVMWTKPVGEEGALRIGQSVKVVNKMAFDVANPDKS"
     gene            566..1777
                     /locus_tag="VLL06_00720"
     CDS             566..1777
                     /locus_tag="VLL06_00720"
                     /inference="COORDINATES: protein motif:HMM:NF004804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HSS29518.1"
                     /translation="MSQTLISRNPDLAKLEDAGLRLWIKTGSAMHLIVEGIPAVSSKR
                     EVMTGSLYSRLELDQNERTAKPLGSHQCWWIGDEMPCDPLGVAMREFLSNPNPEDQGD
                     GIKTLVGYSMKFKEKGAFVSYPDYYQKIRAYVAYFWAPAREIDDNCTPYSDRPVPALV
                     DMQKRVFAYPDAATTRAGIGAATAKLMTERVAIIGLGGTGSYILDLLAKVPIGEVHLF
                     DPDTFQLHNAYRAPGAPTAEDLDKFHKVDWFKSIYSRMHLGIVPHPYALTEQNLGEIE
                     GFDFVFVAVDKPTVRKIILPGLMARKVPFIDVGMGLNLDMNNSVSGICRYTVGLPDFN
                     AHVEQVVSFDDGPQENLYRNIQVADMNMFNAALAVMKWKKLRGFYADSKREHHGLYNI
                     ATGSLVKEDCR"
BASE COUNT          512 a          587 c          630 g          470 t
ORIGIN      
        1 agaatgagca tcgttacatt aatgaaacag tcatcgtaca tagagattgt ttcacaaaag
       61 aggcactgtt gcaagtagta actcattgat ttttaagctc ggcagaggcc gaacggagtt
      121 ttagagatgg agattcaagc cgatcggact ggtcatcagg tccgcgttca catcgaccag
      181 cattcctatg agtcggcgac gccgacgacc ggtgaggcgc tctaccagct ggcgcacatc
      241 cgtcccgaat tgctgctgta taaggaggtc ggcggcgatc gcgaagatca attggtgtct
      301 cgagatagcc aggaggtgca ccttcacgag gatgagcact ttcacagcgc tcccgccagg
      361 catgacgtga cgatcttcgt caatacggag cccttcccgt gggaccgccc caaaattacg
      421 tacgaggacg ttgtgaagct ggcattcccc aatgggccat ttggaggtga cgtccggtac
      481 agcgtcatgt ggacaaagcc ggtcggcgag gagggcgccc tgcggatcgg gcagagcgtc
      541 aaggtcgtga ataagatggc cttcgatgtc gcaaaccctg ataagtcgta atccggacct
      601 cgcgaaactc gaggacgcgg ggcttcgcct gtggatcaaa acgggctccg ccatgcatct
      661 catcgttgag ggcattccgg cggtcagctc caagcgggag gtcatgacgg gctcgctcta
      721 ctcgcgcctg gaactcgatc agaacgagag gaccgccaaa cccttgggaa gccatcagtg
      781 ctggtggata ggtgacgaga tgccatgcga ccctttgggg gtcgcaatgc gggaatttct
      841 gtccaatccg aaccctgagg accagggcga tggcatcaag acgctcgtcg gatattcgat
      901 gaagttcaag gagaagggcg cgttcgtcag ctatcccgac tactaccaga aaattcgagc
      961 ctatgtagct tacttctggg ccccggcccg cgagattgac gacaattgca caccttattc
     1021 cgataggcca gtgccggcat tggtggatat gcaaaagcgc gtgtttgctt accccgacgc
     1081 tgcgactaca agggcgggta tcggggccgc gacagcgaaa ctcatgacag agcgagtggc
     1141 gatcatcggc ttgggtggca cgggttctta catcctcgac ttacttgcca aagttccgat
     1201 tggagaggtc cacctctttg acccggacac tttccagttg cataacgcct atcgggctcc
     1261 gggagccccc acagccgaag atttggacaa gttccacaaa gtcgattggt tcaaatccat
     1321 ctatagccgt atgcacttgg ggatcgtccc ccatccatat gccctcacgg agcaaaactt
     1381 gggggagatc gagggcttcg acttcgtatt tgttgccgtc gataaaccca ccgtccggaa
     1441 aatcatcctc ccgggcctca tggcaagaaa ggttccattc attgacgtcg gtatgggcct
     1501 gaacctcgat atgaataact ctgtgtcggg aatctgccgg tacacggtcg gcttgcccga
     1561 cttcaacgcg catgtcgaac aggtcgtttc ctttgacgat ggacctcaag agaatttata
     1621 tcgcaacatc caggtcgcag atatgaacat gttcaacgcc gcacttgccg taatgaagtg
     1681 gaagaaactc cgaggcttct acgctgacag caaacgtgag catcacggcc tatacaacat
     1741 cgctacggga agtctcgtga aggaagactg ccggtgaagg tctattcgat ccggccccag
     1801 tatgtggagt cgttccccaa actcctcgag gagggagtta tttacatctc ccaacgattc
     1861 agcaccgcgt gccacaactg ctgttgtggc tgtggcacga aaatcgtgac cccaattcgg
     1921 ccgaccgagt attcgctttc agaaaaaggc ggcctcatta cccttcaccc ttctgtcgga
     1981 aactggaact atccatgtca gtcgcactat ctgatcaggg acaatcagat cctctgggcg
     2041 ggaggcatga gtcgtgcgga gatcagtagg ggccgtgccc gagatgatgc cgagaaggtt
     2101 cggtacctcg gccaggcacg gggaggctgg tggtggaagc tctgggcttg gatcaagagc
     2161 ttgtttcgat aaccaacgca ggtccaggtg ggactgtgg
//
LOCUS       DATIPF010000028         2961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_888160, whole genome shotgun sequence.
ACCESSION   DATIPF010000028 DATIPF010000000
VERSION     DATIPF010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888160"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /gene="nagZ"
                     /locus_tag="VLL06_00725"
     CDS             <1..945
                     /gene="nagZ"
                     /locus_tag="VLL06_00725"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HSS29519.1"
                     /translation="NKLQACSPHSPLLISIDQEGGRVSRLPKGFTIFPSCSLLGRSNS
                     TELAYAAGATIAKELRAVGVNMNMAPVLDVNSNPDNPVIGDRAFGTTPDVVSELGIAT
                     AAGLQDNGVVACGKHFPGHGDTNTDSHKELPVVEATRERLEAIEFPPFRRAVQRDVAS
                     LMTAHVLYRALDRELPATLSPAIINDFLRQELRYDGVVLTDDLEMHAIVDHFGVEDAA
                     VRAVQAGCDMVLICKDRDREVTAFEAVEQAVASGTISTERLDLSAARIVRLKNRFVTP
                     YKPVTISDAKLIAGCRTHQALLNSIEQARARVSYSQSP"
     gene            1073..1612
                     /locus_tag="VLL06_00730"
     CDS             1073..1612
                     /locus_tag="VLL06_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HSS29520.1"
                     /translation="MIRTFQGIKPTIPTSCFIEETGVVIGDVVLGEHCSVWFHAVIRG
                     DVHYIRIGERTNMQDLCILHVTHDTHPLVIGNEVTIGHAAILHGCTIKDRVLIGMGAI
                     VMDGAVIGEDSIVGAGALVVEDMIVPPKSVILGSPGRVRRTVSETELAWIKESAENYV
                     KYSSQYLDTSSRTKPGFRI"
     gene            complement(1779..2498)
                     /locus_tag="VLL06_00735"
     CDS             complement(1779..2498)
                     /locus_tag="VLL06_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29521.1"
                     /translation="MKTSRTRLAPATRRLVTEMYGCLDYEALGPVYCYEGGDEFWRAK
                     RVPCGRLGSRVAAELRRKLNRDGRSLYVGAGVAELPALIMETVELGRTVEPYNLRRAE
                     VAALNHVCDMIPLTFHVADAASAKGRFDHLWMVSVLNDPERFPHLSPFSYGRGNPLTL
                     DPLKFAKERRIVRALVARCMGKLAKPGLVTTTTEEVLWIAEWCHRYRVPYRVGKRYYP
                     TALVGDPICFIRIGKGTKEAG"
     gene            complement(2495..>2961)
                     /locus_tag="VLL06_00740"
     CDS             complement(2495..>2961)
                     /locus_tag="VLL06_00740"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HSS29522.1"
                     /translation="FASRLKAEGRELAGAIILECVGFARSEAGTQQIPPGVPIAVPTQ
                     GDFLAIVGNEASRALVTQLEQEARRHASRLKPLSLVVPGRGEALPDTRRSDHASFWDA
                     GYPAVVLTDTANFRNPHYHRATDTVDTLNLEFLSKIIATVTATAIRIAGVRP"
BASE COUNT          626 a          883 c          832 g          620 t
ORIGIN      
        1 aacaagttac aggcctgtag cccccattcg ccgctcttga tttcgatcga tcaagagggc
       61 ggccgggtct cccgtctgcc gaagggcttt acgattttcc catcctgcag cctgttgggc
      121 cgttccaatt caaccgaatt ggcctacgcg gctggggcca ccattgccaa agaactgaga
      181 gcggtgggtg tgaatatgaa tatggcgccg gtccttgatg tgaacagcaa tccggacaac
      241 ccggtcattg gcgaccgcgc gttcggcacg acgccggacg tcgtctctga actggggatc
      301 gcgacggcgg cggggctcca agacaacgga gtcgtggcct gcggaaaaca ctttccaggt
      361 catggcgata cgaataccga ttctcacaaa gaacttccgg tcgtcgaggc aacacgggag
      421 cgattggagg cgatagagtt tccccccttc cgccgtgctg tccagcggga tgtcgcatca
      481 ttgatgaccg cgcacgtgct gtaccgggcc ctcgatcgag aattgccggc cacgctctcg
      541 ccggccatca tcaatgattt cttgcgccag gagctacgat acgacggagt cgtgttgaca
      601 gatgacctcg aaatgcacgc gatcgtcgat cattttggcg tggaggatgc tgcggtccgt
      661 gcggtacagg cgggttgcga catggtattg atttgtaaag atcgggaccg ggaagtcacg
      721 gcattcgaag ccgtggaaca ggcagtcgcg tcgggcacga tttccaccga gcggttagac
      781 ctctcggcag ctcgaattgt ccggctgaaa aaccggtttg tgactcccta taaaccggta
      841 acgatctccg atgcaaaact cattgcggga tgccgaaccc atcaggcatt gttgaattcg
      901 atcgaacagg cccgggcgcg ggtatcttac tcacaatccc catgagggct tctgctgcaa
      961 tcgacgatcc tttgcgacga cacgccttcg atcagcggat ctgcaccctt ccctcatcct
     1021 cctccatgac cccccttgca ggtcgcgggc cggttcccct acagtgaacc gcatgatccg
     1081 cacatttcag ggaatcaaac ccaccattcc cacgtcctgt tttatcgaag agaccggcgt
     1141 ggtcatcggc gatgtcgtgc tgggcgagca ctgcagtgtc tggttccatg ccgtcattcg
     1201 cggggatgtg cactatattc ggattggtga gcggacgaac atgcaggacc tctgtatact
     1261 ccatgtcacc cacgataccc atccgctggt catcggcaac gaggtgacca tcggacatgc
     1321 ggcgatactc catggttgca cgataaagga tcgtgtgctg atcggcatgg gagccattgt
     1381 gatggatgga gccgtgattg gagaggattc gattgtgggg gccggtgcgc tggtggtgga
     1441 agacatgatt gtgccaccga agagtgtgat cctcggatcg ccgggccgtg ttcggcgcac
     1501 cgtatcagaa accgaactcg cgtggattaa ggagtcggcg gagaactacg tgaagtacag
     1561 cagccagtac ctagacacct cgtcaagaac caagcccggc ttccgaattt agcaggatgc
     1621 tgaaaaatcc gccagcttcc ttcttccatg gttcagaccc tcaacgggac ccggccgcct
     1681 caccactcgg cggcgcgcac agacgtggtg ctccttattc gtcgcaccgt gcaccccaga
     1741 ggattgtttg cccgcaagct gctggatttt cggacgcctt acccagcttc tttagttccc
     1801 ttgccgattc gaataaaaca tatcggatcg ccgaccaatg ccgtcgggta gtaccgcttc
     1861 ccaacccgat agggcactcg atacctgtgg caccactcgg caatccacaa gacttcttcc
     1921 gtcgtagtcg tgacgaggcc aggcttcgcg agttttccca tacaacgtgc caccaatgcg
     1981 cgcacgatac gacgctcttt ggcaaacttt agcgggtcga gagtgagagg attgcctcgt
     2041 ccataagaaa atggcgacag atgtgggaac cgctcagggt cattcaacac gctgaccatc
     2101 cagaggtgat cgaatcggcc cttcgcgctg gctgcatcag ccacatggaa cgtgagagga
     2161 atcatgtcac agacatggtt gagagctgcc acttcggctc ttcgaagatt atagggttcc
     2221 actgtccggc caagttctac cgtttccata atcaatgccg gaagctccgc cactccggca
     2281 ccgacataga gactgcgccc gtcccggttc agtttcctcc tcaactccgc ggcgactcga
     2341 ctcccgagac ggccacaagg cactcgtttc gctcgccaaa actcatcgcc gccttcataa
     2401 caatagaccg gtcccagggc ctcatagtcg aggcaaccat acatttctgt cacgagacgt
     2461 cgagttgcgg gggccagcct tgtacgactc gtcttcacgg tcgtacccca gcgatacgaa
     2521 tcgcggttgc ggtcaccgtc gcgatgatct tggagagaaa ttcaagattc aatgtgtcca
     2581 cagtatcagt cgcacgatgg taatgggggt ttcggaaatt cgccgtatcg gtcaacacca
     2641 ccgctggata gcctgcatcc cagaacgagg catggtcgct acgacgggtg tcgggcaggg
     2701 cctcccctcg gcctggcacc accaatgaca ggggtttgag ccggctcgca tggcgccggg
     2761 cctcctgttc aagctgagtg accaacgccc gggatgcttc gttgcccaca atggcaagaa
     2821 agtctccctg cgtcggcacc gcgatcggca caccgggtgg gatctgctgc gtaccggcct
     2881 cgcttctggc gaacccgaca cattccagaa tgatcgcacc ggccaactca cggccttcgg
     2941 ctttcaaacg ggacgcgaac g
//
LOCUS       DATIPF010000029         3774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_976743, whole genome shotgun sequence.
ACCESSION   DATIPF010000029 DATIPF010000000
VERSION     DATIPF010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976743"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VLL06_00745"
     CDS             complement(<1..205)
                     /locus_tag="VLL06_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29523.1"
                     /translation="MINTWWSPQLNALLGSLVVAAGAWLAWDSFSLWEVSLVAAGVAG
                     FLVWQGRTIGRVWAWATLLLGLES"
     gene            complement(565..1293)
                     /locus_tag="VLL06_00750"
     CDS             complement(565..1293)
                     /locus_tag="VLL06_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HSS29524.1"
                     /translation="MTDTLRINKFFTHHGICSRREADRLIESSRVTINQRVACLGDQV
                     SPDDVIARDGQVIPWGTVSLYIKYYKPVGVTTTSESHVPRNIIAEIGHPERIFPIGRL
                     DKNSSGLILLTNDGDIVNEILRTEHGHEREYEVSVDHPFDQTFIDRMAQGVVILERPT
                     NPCRIERLGPIRFRIILTEGRNRQIRRMCQVLGYRVLTLHRVRIMQVTLDGLVPGAWK
                     PLTREERARLFQAVGRGSNQGDTP"
     gene            complement(1311..2012)
                     /locus_tag="VLL06_00755"
     CDS             complement(1311..2012)
                     /locus_tag="VLL06_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HSS29525.1"
                     /translation="MATTRSLNLDGKVDQQAVDAIEAFVAPSVGTGRFVVTLTGEHTG
                     ALGAIRAHARRYPDLCVLQIDAHGDLRKAYQGNPYSHASVMARVVDDGLPLVQVGIRS
                     ISPEEIDRIQSTDRIATFFAAEILDPSGPYEGKASRWIPDVVKACRGPVYLTFDCDGL
                     DASLVPALGTPEPGGLGWYDTVNLVTALANGPGILGMDISEIAPIEGFVAPQFCIARL
                     IYRMLGRIKAGRRVH"
     gene            complement(2022..2243)
                     /locus_tag="VLL06_00760"
     CDS             complement(2022..2243)
                     /locus_tag="VLL06_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HSS29526.1"
                     /translation="MTLPAGWEGIDQNFLGLEEPWCHPDRAGVYVLPAPYEHTSSYVQ
                     GSDRGPSAIIEASQQVELYDETLRCEPYR"
     gene            complement(2267..2833)
                     /locus_tag="VLL06_00765"
     CDS             complement(2267..2833)
                     /locus_tag="VLL06_00765"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HSS29527.1"
                     /translation="MVPTQMFLTRGVGVHKEKLASFEQALRSAGVAYCNLVTVSSILP
                     PNCKIIPRARGEKLLNPGEITFCVMARSETNERNRLTSASIGLAVPTDRRTYGYLSEH
                     HAHGETDEETGEYTEDLAAQMLATTLGVEFDPNIAWKEREQVFKMAGKIVRTLNITQS
                     AIGKPNRWTTVIALAVFIPEENIPKRRR"
     gene            complement(3256..>3774)
                     /locus_tag="VLL06_00770"
     CDS             complement(3256..>3774)
                     /locus_tag="VLL06_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSS29528.1"
                     /translation="RLKAGDPSDDSTVVGPLIDPAAAHRVETWIGEAVSQGARVLLGG
                     KRMGSVMEATVLSHVTPTMKVSCQEVFGPVVTVTPYRHFGEAITALNQSDYGLQAGVF
                     TQDVNKIFYAFRHIEVGAVLANEIPTFRADHMPYGGVKDSGLGREGVQASIEDMTEPR
                     MLVLNLKPPAGA"
BASE COUNT          845 a         1115 c         1017 g          797 t
ORIGIN      
        1 gactctccag accaagtaac agggtggccc aagcccagac ccttccaatc gtgcgtccct
       61 gccagacgag aaatcctgcc acgcctgctg cgacgagaga aacctcccaa agtgagaatg
      121 agtcccaggc caaccaggca ccagctgcca cgacgaggct accaagcagg gcattgagtt
      181 gggggctcca ccacgtgttg atcatccggg tattgtgctc cgtgaacgat gcgcggacta
      241 gtctgagttc tggctgcgcg gttgtcaatg gaggtgcccg cgagcgagag agggacagga
      301 caaggccaac aagatgctga acacgtgcat cagcgtcatt cttgcgtcac tcagaagctc
      361 aacgttgaat gtgatggggg accatggcca acccacgcac aagtctcctg ctcgtcggtg
      421 taagtagcac tctgagagct tctttcagtt gatcacccct agagcaaacc tgagcctact
      481 catctcccaa gcccatcctg cttccgctga ctgatggagt tcctacgcga tacttcgtcc
      541 ggagcagttc ggtcccagat catcctatgg cgtatcaccc tgattgctgc ctcgcccgac
      601 agcctgaaag agccgtgcgc gttcctctct ggtgagcggt ttccacgctc caggcaccaa
      661 cccgtcaagt gtcacctgca tgatccgaac acgatgcagc gtgagtaccc gataccctaa
      721 cacttggcac atgcgccgaa tctgtcgatt gcgcccttcg gtaagaatga tacggaatct
      781 tatgggaccg agtcgttcga tacggcaggg gttggtcgga cgctcaagaa tgacaacccc
      841 ttgtgccatc cggtcgataa acgtctgatc gaatggatga tcgactgaca cctcgtattc
      901 ccgttcatgt ccgtgctcag ttcggagaat ctcgtttaca atgtcaccgt cgttggtgag
      961 aagaataagg ccggaggagt tcttgtcgag tcgaccgatt ggaaaaatcc gctccggatg
     1021 gccgatctcc gcgatgatgt tacggggcac atgcgactca ctggtcgtcg tcacacccac
     1081 cggcttgtaa tacttgatat agagcgagac cgtcccccag ggaattacct gcccatctcg
     1141 tgcaatgaca tcgtcaggac tcacctgatc ccccaagcag gcgacccgtt gattgatggt
     1201 tacgcgacta gactcgatca gccgatcggc ctctcgtcga gaacagatgc cgtggtgggt
     1261 aaaaaattta ttgatgcgaa gcgtgtcggt cacagcgtcg gtatgccgtg ttagtggacg
     1321 cgacggccgg ctttgatccg tcccagcatg cggtagatca atcgggcgat acaaaattga
     1381 ggcgcgacaa acccctcgat cggggcaatc tcgctgatat ccatcccgag aatgcctggg
     1441 ccattggcca aggctgtcac gaggttcacg gtgtcatacc accctaaccc accgggctcg
     1501 ggagtgccga gcgcaggaac caacgaagca tcaagtccgt cacaatcgaa ggtcaaatag
     1561 accggcccac gacaggcctt caccacatcg ggaatccagc gggaggcctt gccttcgtag
     1621 gggccggacg ggtcaagaat ctcggcggcg aagaacgtgg caattcgatc ggtgctctga
     1681 atgcgatcga tctcctcggg agagatggaa cggataccga cttggaccaa cggtaatccg
     1741 tcgtcgacca cgcgggccat cacgctggcg tggctatacg gatttccttg ataggccttc
     1801 cgcaagtcgc catgcgcatc gatttgcaat acacaaagat ccggatagcg tcgggcatgg
     1861 gcccggatag ccccaagcgc gccggtatgt tccccggtca acgtgacaac aaaccgcccg
     1921 gtaccgacag agggagcgac gaacgcctca atggcatcca ccgcctgctg atcgactttg
     1981 ccgtcaagat tgagcgagcg agtggtggcc accccacccc actatcgata cggttcacac
     2041 cgaagtgttt catcgtataa ctcaacttgt tgagacgctt cgatgatagc tgaggggcct
     2101 cgatcggacc cctgcacata gctggaggta tgttcgtagg gtgcgggcag gacatagacg
     2161 cccgcccgat caggatggca ccaaggctcc tcgagcccga ggaaattctg gtcgatgcct
     2221 tcccatccgg cgggaagtgt catgggcgta tactcacgca gctggtctat cgacgccgct
     2281 ttggaatatt ttcttcagga ataaacacag ccaatgcaat caccgtcgtc cagcgattgg
     2341 gttttccaat tgcggactgg gtgatgttga gggtccgaac gatcttcccg gccattttga
     2401 agacttgttc ccgttctttc cacgcgatgt tcggatcgaa ctcgacaccg agcgtagtgg
     2461 ccaacatctg tgccgccaag tcttccgtat actcacccgt ctcttcatcg gtttcgccat
     2521 gcgcatggtg ttcggacaaa tagccgtatg ttcgacgatc cgtcgggacc gccaacccga
     2581 tcgacgcgga ggtcaggcga ttacgttcgt tcgtttccga ccgtgccatc acacagaatg
     2641 tgatttcacc gggattcaat aacttctcac cgcgcgcacg ggggataatc ttgcaattcg
     2701 gtggaagaat cgacgacacc gtgacgagat tacaataggc gacacctgca ctacgcaagg
     2761 cttgttcgaa ggaggccagt ttttctttgt ggactccgac gcctcgcgtc agaaacatct
     2821 gcgtaggtac catcgcgtct tctccttact tcgtccgacc aaccgggtga cggacacctt
     2881 gttaactgaa ttgcgcgccc acagcaaact tgatatcggc acagggtcgc tgttgtacca
     2941 agattggcaa cgcttcgcaa tatcctgcgg caatttttct gacaggatgc tgaaaaagtc
     3001 tctcaactca cccgcccgac cccggcgcgc caagacgcgc cttttcctat gcatcattct
     3061 cgcctcgaag gcatcctcaa cgtagccctc gcggaagaca agctgtcttg gcagcttggg
     3121 gtgggtgggg gagaagaggg acgcctccgg tgattccatt agttgcggtc ttgctgaacg
     3181 gccattgtga gcatcctgca gaagtatgtt ccgcatgtgc tacacgtgca caccctgaag
     3241 aaaatccgca tcccgttagg cgccggcagg cggcttgaga ttcaacacca gcatgcgggg
     3301 ctccgtcatg tcctcgatcg aagcctgaac cccttcacgc ccgagcccgg agtccttcac
     3361 gccgccatac ggcatgtggt cggcgcgaaa cgtcggaatc tcatttgcca gcacagcccc
     3421 aacttcaata tgacgaaagg cataaaaaat tttattcaca tcctgagtga acaccccagc
     3481 ttgaagccca taatcagact ggttgagtgc cgtgatcgcc tcgccgaagt gacggtaggg
     3541 agtcacagtc accaccggcc caaacacttc ctggcaagag actttcatgg tcggtgtgac
     3601 gtgcgagagc acagtcgcct ccatgaccga acccattcgt ttgccgccca aaagcacacg
     3661 tgctccttgg gacacagcct ccccgatcca tgtttccaca cgatgggctg ctgctggatc
     3721 gatgagcggt ccaactaccg tcgaatcgtc gcttggatcg ccggctttca aacg
//
LOCUS       DATIPF010000030         1508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_4005, whole genome shotgun sequence.
ACCESSION   DATIPF010000030 DATIPF010000000
VERSION     DATIPF010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4005"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..373)
                     /locus_tag="VLL06_00775"
     CDS             complement(125..373)
                     /locus_tag="VLL06_00775"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HSS29529.1"
                     /translation="MLRIPSRVVFPFGYQISIRQLSDTDMDRRDPNADGIWDDATKTI
                     YLRKRLPVTRRRYILAHELGHAWLDWQHRHLDNGKAKT"
     gene            719..>1508
                     /locus_tag="VLL06_00780"
     CDS             719..>1508
                     /locus_tag="VLL06_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HSS29530.1"
                     /translation="MMYRVLVLGAGKIGSLVACLLSESGDYEVSLGDISLDASKRFVE
                     DLGLSRVTPLLLDARHPAAISAHLKADRFDAVLSGLPYFCNPVVAELARAHRLHYFDL
                     TEDVEVTNQVKALSTDSSQAFVPQCGLAPGFISIAAHDLITHFDMVDTVKMRVGALPV
                     HPSNALKYSLTWSTDGLINEYGNICYGIEAGEKVSLLPLEGYETIEVDGLLYEAFNTS
                     GGLGTLADTYAGKVRTMNYKTLRYPGHCDKISLLMKDLKLNEDRE"
BASE COUNT          308 a          383 c          454 g          363 t
ORIGIN      
        1 gcgcacggtg cgacgaataa ggagcaccac gtctgtgcgc gccgccgagt ggtgaggcgg
       61 ccgggtctcc attgaggatt tcgatgagcc gagaacgaag ctggcggact ttttcagcag
      121 cctgctatgt tttggctttg ccgttgtcga gatgccgatg ctgccagtcg agccaggcat
      181 ggccaagttc gtgggcgaga atgtagcgcc gtcttgtcac aggcaagcgc ttgcgaaggt
      241 aaatggtttt tgtggcgtcg tcccagatgc cgtcggcatt cgggtcacga cgatccatgt
      301 cggtatcgga cagttggcgt atggagatct gatagccgaa cggaaacacg actcgcgaag
      361 ggatgcgcaa catgactgct gctcctgtgt gtgtgtgtta gcgtagcata aagtcctttc
      421 ccgcttcacg ggggatgtgt taagtaagat caataggtta taagtatttt ccactcaaca
      481 atgtagcgcc ttcattgggc tgcccttgtc aaaatgtcca gactcttgca ggcggggctg
      541 ggtcttgggc gctttcgtcg aggtgggggg ttttcatcga atcttcgccg ggcgatcttg
      601 cggggttgtc gcctaattat gccacaggtg tgagccatcg aaactctcgc ctgccgcaat
      661 agctttttcg catcatgcga gagcaggagt atacttatat taacgagcag ggaggccgat
      721 gatgtatcgt gtgctagttc tgggtgctgg aaagatcgga tccctggttg cctgcctctt
      781 atcggaaagc ggagactatg aggtcagtct tggtgacatt agcctcgatg cttcgaagcg
      841 gtttgttgag gacctcggtc tctctcgcgt gactcccttg cttctcgacg cccggcatcc
      901 cgccgccatc agtgcccatc tgaaggcgga tcgatttgat gcagtcctgt cgggccttcc
      961 atatttctgc aatcccgttg tggccgaatt ggctcgggcg caccggcttc actattttga
     1021 cctgacggag gacgtcgagg tcacgaatca ggtcaaggcc ctgagcaccg actcatcgca
     1081 agcctttgtg ccccagtgcg gcctggcccc ggggttcatc agcatcgcgg cgcacgacct
     1141 gatcacccat ttcgatatgg tggatacggt caagatgcgg gtcggagcct tgccggttca
     1201 tcccagtaac gccctcaagt attcgctgac ctggtccacc gatggattga tcaacgagta
     1261 cggcaatatc tgttacggaa ttgaagcggg agagaaggtg tcgctcctgc ctctcgaagg
     1321 gtatgagacg atcgaggtcg acggactcct gtacgaagca ttcaacacgt caggtggatt
     1381 gggaacgttg gcggatacct acgcagggaa agtccggacc atgaactaca agacgctgcg
     1441 gtaccctggc cattgcgaca agatttcgct tttgatgaaa gatctcaaac tgaacgaaga
     1501 tcgtgaga
//
LOCUS       DATIPF010000031         3502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_622860, whole genome shotgun sequence.
ACCESSION   DATIPF010000031 DATIPF010000000
VERSION     DATIPF010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622860"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VLL06_00785"
     CDS             <1..536
                     /locus_tag="VLL06_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSS29531.1"
                     /translation="STILDLSKMDAGMMEYRIVATDLKRVTDMSVNKVQLLADAKHVQ
                     LLLEAPAQRVWVKADALRIEQVLDNLLSNALKFSSEGGIVKVLMKPDSKAGVLEVSVS
                     DGGPGIQAEDLPFIFERFYQGRTNAKHATPGSGLGLALAKKVVEAHGGRIWIESESKK
                     GTTVRFILRLTKPGEAA"
     gene            533..1315
                     /locus_tag="VLL06_00790"
     CDS             533..1315
                     /locus_tag="VLL06_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29532.1"
                     /translation="MTALYRGVASWVFPCLFLAGCAAWSPAPQFSQPYFVVDAGEAKA
                     LHALAKKQELLATKCAEHNSCDHVYFTRALLGLYESRDNASKYFEKVIAVAPRSQVAS
                     FSKLWLQLIEYHAAPVEHSWFQSVIAAPAISESQAILAQASDRLVRDLLDRELTIQQL
                     RAMKDADAQSLENLQRDLAERERKIDSVNGKKDTARGSAETGIVLSLQKQLTDRDKKI
                     EELTSQLEALKRIDQEMRDKVRPIRPPSTVVPPPTAPEPATP"
     gene            1327..2745
                     /locus_tag="VLL06_00795"
     CDS             1327..2745
                     /locus_tag="VLL06_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSS29533.1"
                     /translation="MEKEHILVVDDEEGLLHLVKMRLTAMGFAVTGCTTGREALTAAK
                     KDRFDLAITDLRLGSEDGLDVTEELLRIHPGLPVIILTAHGSIPNAVEAMQRGAFGYL
                     TKPFDDKELKTKIEEGLSPQRMRGEIQRLKSLVNELYGMDNIVARSAAMQRILQQVVQ
                     VADSDATILLFGETGTGKEVLARVIHANSRRSKGPFVALNCAAIPETLFESELFGHVR
                     GAFTSAHGAKRGLFQSANGGTLFLDEIGEIPLSMQVKLLRAVQEREVREVGSEISTKI
                     DVRIIAATNKDLGEAVKNGSFRNDLYYRISVVPLFIPPLRDRRDDIPLLAQHFLTTSA
                     KRANKILRGFTPAALNRLVTHPWPGNVRELENVVEKAAVMTRQDMITPDLLPSMGTSP
                     DAPLKPLTEAKEEFEKTYLKNVLQLTGGNISRAAQFAGRYRADFYKMLKKYGLHPSTL
                     KAKAEADLEELEEDGDLTEAER"
     gene            complement(2850..3197)
                     /locus_tag="VLL06_00800"
     CDS             complement(2850..3197)
                     /locus_tag="VLL06_00800"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS29534.1"
                     /translation="MTGFVMRKNHRLKKLVPVRYLGDGIAGEGIIKDLSLSGSYITGN
                     VLVSVGVELALQIFVPGDPVPLLIDRAIVKWVKGSEFGVDFDTPPPEIAERITQVIST
                     LIKTGYGSSRNKA"
BASE COUNT          848 a          934 c          958 g          762 t
ORIGIN      
        1 tctccaccat tttggacctc tccaagatgg acgccgggat gatggagtat cgcattgtcg
       61 cgacggatct caagcgtgtc accgatatgt cggtcaacaa agtccagctc ctcgccgatg
      121 ccaagcatgt gcagttgtta ctggaagcgc ctgcacagcg tgtgtgggtc aaggcggatg
      181 cgcttcgaat tgaacaagtg ctcgacaatt tgctctcaaa tgccttgaaa ttcagctcgg
      241 aagggggcat tgtaaaggta ctcatgaagc cggattcgaa agcgggagtc ctcgaagtga
      301 gcgtgagcga cggaggaccc ggtattcaag ccgaagacct gccctttatt ttcgaacgct
      361 tctatcaggg gcgcacgaat gccaaacatg cgacaccagg cagtggcctc gggttggcac
      421 tggcaaaaaa agtggtcgaa gcacatggag gtcggatttg gatcgaaagc gaatccaaga
      481 agggaacgac tgtacgattt atcctacgcc ttacgaaacc aggagaagcg gcatgacggc
      541 cctctaccga ggcgttgcct cctgggtttt tccctgtcta ttccttgctg gatgcgcggc
      601 ctggtcgccg gctccccaat tttcacagcc ctactttgtg gtagatgcgg gagaggcaaa
      661 ggctctccat gcgttggcga aaaaacagga gcttcttgcc acgaaatgtg ccgagcacaa
      721 ttcctgcgac catgtctatt ttacgcgcgc cctgctgggg ctctatgaaa gtcgagataa
      781 cgcctcaaag tacttcgaga aagtcatcgc cgttgcacca agaagccagg tggcctcctt
      841 cagcaagctc tggttacagc tgatcgagta tcacgccgct cctgttgagc attcctggtt
      901 ccaatcggtt attgcggccc ccgctatttc ggagagtcag gcgatcttgg ctcaagcatc
      961 cgatcggctg gtgcgggact tgcttgatag agagttgacc attcagcagt tgagggccat
     1021 gaaggacgcc gacgcgcaat ccctggaaaa cctccagcgg gatttagccg agcgtgaacg
     1081 gaagatagac tcggtcaacg gcaagaaaga cactgcaaga ggttcggccg agaccggcat
     1141 cgtgctgtcc ttgcagaaac aattgacgga tcgcgataag aaaatagagg aattgacgag
     1201 ccaattggag gcattaaagc gtatcgacca ggaaatgcgg gataaggttc gtcctatccg
     1261 tcccccatcg acggttgtac cgcctccgac tgcgccggaa ccggcgaccc cgtagcaagg
     1321 agtttcatgg aaaaagaaca tattctcgta gtcgatgatg aagagggatt gttacacctg
     1381 gtgaagatgc ggttgacggc catgggcttt gccgtcacag gctgcaccac cgggcgtgag
     1441 gccctcaccg cagccaagaa ggaccggttc gacctcgcca ttaccgatct ccggctgggt
     1501 agcgaagacg gattggatgt gacggaagaa ctactgcgga ttcatccagg ccttccggtg
     1561 atcatcctga cggcccacgg cagcatcccc aatgccgtag aagctatgca gcgaggggcc
     1621 tttggatatc tcaccaaacc gttcgacgac aaagaactca agaccaagat cgaagaagga
     1681 ctctcgccgc aacgaatgag aggggaaatt cagcgactca aatctcttgt caacgaattg
     1741 tatggcatgg ataacatcgt cgcgcgaagt gcggcgatgc agcgtatcct ccaacaggtg
     1801 gtgcaggtgg cagattcgga cgccaccatt ctcctcttcg gagaaactgg gaccggcaaa
     1861 gaagtgctgg cacgtgtcat ccatgcgaac agtcgacgga gcaaaggacc gtttgtcgcc
     1921 ctcaattgcg cggccattcc tgaaaccctt tttgagtctg aactctttgg acacgtgcgc
     1981 ggcgcgttta ccagtgccca tggcgccaag cgaggcttgt ttcagagcgc caatggcggc
     2041 acgctctttc tcgatgagat cggtgaaata ccgctctcga tgcaggtcaa actgttgcgg
     2101 gccgtgcagg aacgggaagt ccgcgaggtg ggttctgaaa tctcaacaaa gattgatgtg
     2161 cggattatcg ccgcaacgaa taaagacctc ggtgaagcag tcaagaacgg atcctttcgg
     2221 aacgatctat attatcgtat ctcggtcgtc ccgttgttta tcccgcccct tcgggaccga
     2281 cgcgacgata ttcccttgtt ggcgcaacac tttctgacca cgagtgcaaa gcgcgcgaac
     2341 aagattttgc gtggttttac accggccgcc ctcaatcgat tggtcacaca tccttggccc
     2401 gggaatgtcc gagaattgga gaatgtcgta gaaaaggccg ccgtcatgac gcgacaagac
     2461 atgattacgc cggatctctt gccctcaatg ggaacatcgc cggatgcgcc cctcaagccg
     2521 ctgactgaag ccaaggaaga gtttgagaaa acctacctca agaacgtgct gcaactcacc
     2581 ggcggcaaca tctcgcgtgc tgcccaattt gccggacgct atcgggcaga tttctacaag
     2641 atgctcaaga aatatggcct acacccctct accctgaagg ccaaagcgga agccgatctt
     2701 gaagagctgg aagaggatgg cgacctcaca gaggccgaac gctagagatc cgtgcgtttc
     2761 tttgcaaaga gcgaagacaa gagcgggtaa tgaggagccg aaggaggtct ttcaaagttt
     2821 gcgtgaggcc gtgcacctgc cagtttcact tatgctttgt tgcgagatga gccatagcct
     2881 gtcttgatca gtgtggagat gacctgtgtg attcgctctg caatttcagg cggcggagta
     2941 tcaaagtcca ctccgaactc agatcctttg acccacttca cgatggcgcg atcgattaac
     3001 aagggcaccg gatctcctgg cacaaaaatt tgcagggcaa gttccactcc gactgagacc
     3061 agtacattcc cggtgatata gctcccgctg agtgaaagat ctttgatgat tccttcaccg
     3121 gcaatcccgt ctccaagata acgcaccggc actaattttt tgagccggtg attcttccgc
     3181 ataacaaacc cagtcattct gtctcctttc tcgatcgggc attcttgttt cttgactgct
     3241 gctattgtac tactcaactg aaaagttgga cagaaaatta cccagtgagc cgtaacacct
     3301 tctccgagac ggtgaggatc gtttccgacg tgactcccaa tttatatctc cgatcggttc
     3361 aacttagtct ctgaggcgtc tcgaggacta accgatggat ggatcttcag caccaccaag
     3421 acttttcgag cataggggcg gcgcgtgatc aacaactttg agtaggcgta ggcatcgcca
     3481 ctgccggtgc ccgccggagc ac
//
LOCUS       DATIPF010000032         4807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_269297, whole genome shotgun sequence.
ACCESSION   DATIPF010000032 DATIPF010000000
VERSION     DATIPF010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269297"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1793
                     /locus_tag="VLL06_00805"
     CDS             <1..1793
                     /locus_tag="VLL06_00805"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HSS29535.1"
                     /translation="TRAPKTTANHVTAWWDASQLYGYDERSRQRVKRDPNDQAKFLLA
                     LPSKGASVGEKLGYLPVFEAEDPINPEWSGQEATAFPDNWSIGTSFYHNVFAREHNAF
                     VREFRKQTSLTPNADSGLRNRAILDPVIRYKDVTPDELFEVARLVVAAEIAKIHTIEW
                     TTQLLYNEPLNRGMNANWSGVFANQKVVAEALKEVVRRLDDTHDAKKTNSLYAALAGG
                     PGIFGLGNRVYDGVPLVGLIDPDKEDRWDFKNDDHVNGGVNHFGSPFNFPEEFITVYR
                     LHPMLPDLIEFREWNKEPNVIRHKVPVIETFRGKATEAMQQKGLANWALSMGRQRLGE
                     LTLQNHPQFLQNLKMPRLQSSTQQIDVAALDLIRDRERGVPRYNEFRRQYGLKQLTRF
                     DDFVDTRVPKGSSARIKQEQLVKMLREVYGQHHCNESKVITKAQLNDDKSPINDCLGH
                     PNGSLIDNIEDVDTVVGWLAEFRRPHGFAISETQFVVFILNASRRLFSDRFFTSSFRP
                     EFYANLGVEWVMHNGPGPEMMEQGMPNGHEQPVSPLKRVLLRTMPELDGELQGVVNVF
                     DPWARDRGEYYSLQWKARPGAEEDEAFAKK"
     gene            complement(2317..2508)
                     /locus_tag="VLL06_00810"
     CDS             complement(2317..2508)
                     /locus_tag="VLL06_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29536.1"
                     /translation="MWLLVIVLLNIVPGLNKVTVLNTFATAEECQIERNRIGFEMAAA
                     YPNERDFVVACQLNPKHSS"
     gene            2848..3384
                     /locus_tag="VLL06_00815"
     CDS             2848..3384
                     /locus_tag="VLL06_00815"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HSS29537.1"
                     /translation="MKHCTLKENCRTLSPPYVLGFWSLILLLVLNSGSVYAEWMAVEK
                     DYLSPGLQTVYIDPDSIRREGNLVMLWQLIDFKWMQGSARGPARFMSTKTHKQFDCAG
                     KRIRLLAFTEFSHSMGTGIPVGGHVDSGHWMPVEPDSVNHALWEVACDNRGNPVGSLF
                     DRAPLGLALSESRSGLVH"
     gene            3671..3991
                     /locus_tag="VLL06_00820"
     CDS             3671..3991
                     /locus_tag="VLL06_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29538.1"
                     /translation="MKHPPVLTLFLGFVLVSGCSSADVLDLVSRDPGQDHGAIASYYT
                     REAALSRQQIEELTDRAVLYEGLFGRESDWVTGTKFLVKFYQEMALEQDRLADLHLKL
                     SRKK"
     gene            4066..>4807
                     /locus_tag="VLL06_00825"
     CDS             4066..>4807
                     /locus_tag="VLL06_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HSS29539.1"
                     /translation="MTRFTLTTLALAVLLLTAACGTTIQPGQRGLFWHPFTEGLSTQP
                     LKDGFYWQAPWNDVYRYDIRWQSHTEDIDALSADDLQVSIKAAIILRPIPEELYFLQQ
                     EVGPDYYPRVVKPQFMAAVRSVVSGYNMVTVPEMSTEIASKVRAVVVENLKGRHLEIQ
                     NIALSDVDFPQIVLRAIEQKQAKEQEKEQKEFELTIASKDAEIARTRAKGEGDAIRIR
                     AEGEAEGLRIRAIGQSQAQETITKTLTPS"
BASE COUNT         1127 a         1253 c         1376 g         1051 t
ORIGIN      
        1 tcacgagggc accgaagacc accgcgaatc atgtcaccgc ctggtgggac gcttcccaac
       61 tctatggata tgacgagcgg tccaggcaac gagtgaagcg tgaccccaat gaccaggcaa
      121 aattcctgct ggctctcccg agcaaagggg catcagtggg cgagaagctg ggatatctgc
      181 cggtcttcga agcagaggat ccgatcaatc ctgaatggtc cggtcaggag gctacggcct
      241 ttccggacaa ctggagcatc gggaccagct tctaccacaa tgtctttgcg cgagagcaca
      301 acgcttttgt gagagaattc cgcaaacaga ccagtctcac ccccaatgcc gacagcggct
      361 taagaaatcg cgccatcctc gatcctgtca tccggtacaa ggacgtcacg ccggacgaat
      421 tgttcgaagt tgcccggctg gtcgtcgcgg ccgagattgc caagatccac accatcgaat
      481 ggaccacgca actgctctac aacgaaccgt tgaaccgtgg catgaacgcc aactggagcg
      541 gggtctttgc aaatcagaaa gtggtggcgg aggcgctgaa ggaagtcgta cgccgactcg
      601 acgatacgca tgatgcgaag aagacgaaca gtctctatgc agcgctggcc ggcggacccg
      661 gcatcttcgg attgggtaac cgggtttatg atggcgtgcc actcgtgggc ctgatcgatc
      721 ccgacaaaga agaccgttgg gacttcaaaa atgacgatca tgtcaatggg ggtgtgaacc
      781 atttcggctc ccccttcaat ttccccgaag agttcatcac ggtgtaccga ttacacccca
      841 tgttgccaga cctgatcgaa tttcgtgaat ggaacaaaga gccgaacgtc atccggcaca
      901 aggtgcctgt gatcgaaacc tttcggggca aggcgactga ggctatgcag cagaaaggtt
      961 tggctaattg ggcgctcagc atgggccggc agcggctggg tgagctgaca ctgcaaaacc
     1021 atccgcagtt tttgcaaaat ttgaaaatgc cccgcctgca atcctccacg caacagatcg
     1081 acgtggccgc gctcgatctc atccgagacc gggagcgggg agttccgcgc tacaacgaat
     1141 tccgtcggca gtatgggttg aagcagctga ccagattcga cgacttcgtc gatacgcgtg
     1201 tgcctaaggg ttcttcagcc aggatcaagc aggaacaact cgtaaaaatg ttacgtgaag
     1261 tatatgggca acaccattgc aatgagtcaa aggtcatcac caaggcgcag ctgaacgacg
     1321 acaagtcgcc aatcaacgac tgtctggggc atccgaacgg gagcctcatc gacaacatcg
     1381 aggacgtcga tactgtagtc ggatggctag cggaattcag acggccgcat ggctttgcca
     1441 tttctgaaac gcagttcgtc gtgttcattc tcaatgcctc gcgccgcctc tttagtgacc
     1501 ggttcttcac ctccagtttt cgcccggagt tctacgccaa cctcggcgtc gagtgggtga
     1561 tgcacaatgg accgggtcca gagatgatgg agcaaggaat gcccaatgga cacgagcaac
     1621 cggtctctcc tctgaaacgc gtgttgctgc gaacgatgcc ggaactggac ggtgagctcc
     1681 agggggtggt gaatgtcttc gatccctggg cgcgtgatcg cggggagtat tacagcctcc
     1741 aatggaaagc caggcctggg gcagaggaag atgaggcgtt cgccaagaag taagagatga
     1801 gggcagcctg ttgatctcct gtactcgcgc aacgcgcggt cgcagactcc cctcgttgga
     1861 cgcgcgcagt gggagatcaa acaggccgcc ctcatgtgaa aagtaggcga actagaaagg
     1921 agatagaaga gggggggcgg tcaggtgccc tccttgctcg cagagccccc acgataaaac
     1981 tgtggtcgct cgatgcgcgc agtggagggc aacctcgacc gctcccttta gtagaggttt
     2041 ggagcagagg aggggatgag gctgatgatc ttctgtgctt gcggaacgcg cacgatgaga
     2101 aggtgtttgc tcgacaggcg tagtgcaacc aacccgggtg ttattccgga agagaaaagc
     2161 aagcgagttt ggaaggattc atgagatgga tatgcggtgg cctttgccat tgagcaatta
     2221 gggtgcagct tgctcggttt catgaaggag caaataggtg ggcgctcatt ttctgtttcg
     2281 acgacacgga ctcttccgta ttgcttccag ccgttcttat gagctgtgct ttggattcaa
     2341 ttgacaggca accacaaagt cacgttcatt tggatacgcg gctgccatct cgaagccaat
     2401 gcgatttcgt tcaatctggc attcctcagc cgtggcaaag gtattcagta ccgtgacttt
     2461 attgagcccg ggcacgatat tgagtagaac gatgacgagt aaccacatag agctcctttc
     2521 agccgctcca gcggcggtgg gcccgccttc gggcaaatcc tccacccctc ccgtcgttct
     2581 ccgtgcagtg cttcgacacg taccatactg agccattttt caatgtgctg ccggcctgca
     2641 ctcagggttt cttgttactg attgctgata acacttctgg ggctgaagtc aatacttgtg
     2701 ttgaaaaccc gatgaggagc gagaaccatt tccgcctcgc ccacgcgcgc ggcattgcgt
     2761 ggttgcctcc gcctcgttag aattatcatg tgagtgttct atccgagatg ggttgatcca
     2821 atgtggcact tcgggcccgc atcagatatg aaacattgca cactcaagga gaactgccgt
     2881 acgttatcgc caccctatgt cttgggtttc tggtcactga tcctactgct ggtgttgaat
     2941 agtggctccg tatatgcgga atggatggca gtggagaaag actatctctc accaggacta
     3001 cagactgtgt acatcgatcc agatagcatt cgcagggaag gaaatcttgt gatgctgtgg
     3061 cagttaatcg actttaagtg gatgcaaggg agtgcgagag gacctgccag attcatgtcg
     3121 acgaagaccc ataagcagtt cgattgcgcg ggaaagcgca ttcgcttgct tgcattcacg
     3181 gagttctcac actcaatggg aaccggcatt ccagttggtg gacacgtaga cagtggccac
     3241 tggatgcccg ttgagccgga cagtgtgaat cacgctctgt gggaagtggc ttgcgacaat
     3301 aggggaaatc cagttgggtc gttgtttgat cgtgcccctc tcggtttggc gctatccgaa
     3361 agtcgttccg gcctcgtcca ctaacacagc ttgatctcct cctcaaaatc ggttgatcca
     3421 acgtgggact tcgcctacgc tgtactgatg aatttgtact gatgaattat tgtacatccc
     3481 agctgggata ctgctatagg gcgcagattc tgaagagcga ggttcactgc gagtcgagag
     3541 ctgatattgc gttggaagca agcgagtgcg ataataaaaa tgcattaccc atctttaccg
     3601 gggccagggc tactggtttc acgcatgggc cctccgtgcg tgtgtccctg gtggagtaag
     3661 gagaactgtc ttgaagcacc caccagttct cacccttttc cttggtttcg tgctagtgtc
     3721 tggctgttcc agcgcggacg tgctggatct cgtctcgcga gatcctggcc aagaccacgg
     3781 agccattgca tcctattaca cccgcgaggc ggcattgtca cgccaacaaa tcgaggagtt
     3841 gaccgatcgg gccgtgctct atgaagggct cttcggacga gagtctgact gggtgacggg
     3901 cacaaagttc ttagtgaagt tctaccaaga aatggcactc gagcaagatc ggctcgccga
     3961 tcttcacctg aaactgagca gaaagaaata ggtctctcaa tcagcccgca cgagaacgaa
     4021 gccacaaatc tgccacgcat ggcgtggccg tactgggagg aagctatgac acgattcact
     4081 ctgacaactc ttgcgctcgc cgtactgctg ctgaccgcag cctgtgggac caccatccaa
     4141 ccaggccagc gggggttgtt ctggcatccg ttcacagaag ggctttctac ccaaccgctc
     4201 aaggacggat tttattggca ggcaccttgg aatgacgtat atcgctatga tattcgatgg
     4261 cagagccaca ccgaagacat tgatgctctg agtgcagatg atcttcaggt gtccatcaag
     4321 gcggccatca tcctccggcc cattccggaa gagctgtatt ttttgcaaca agaagtgggc
     4381 ccggactatt accctcgggt cgtcaaaccc cagtttatgg cggcagtgcg cagtgtggtc
     4441 tccggctata acatggtgac ggtgccggag atgagcaccg agattgcgag caaagtcagg
     4501 gcggtcgtgg ttgagaatct caaaggtcgt cacttggaga ttcaaaatat tgccttgtct
     4561 gatgtcgatt ttcctcagat tgtcttgcgt gcgattgagc agaaacaggc gaaggaacag
     4621 gaaaaagaac agaaagaatt cgaactgacc atcgcctcca aagatgccga gattgcccgc
     4681 acgcgggcga aaggcgaggg ggatgcgatc cggattcgtg ccgagggtga ggcagagggg
     4741 ctccgcatcc gggccattgg acagtctcag gcgcaggaga ccattacaaa aaccctcaca
     4801 ccgtcct
//
LOCUS       DATIPF010000033         1511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1595813, whole genome shotgun sequence.
ACCESSION   DATIPF010000033 DATIPF010000000
VERSION     DATIPF010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595813"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VLL06_00830"
     CDS             <1..631
                     /locus_tag="VLL06_00830"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29540.1"
                     /translation="QFMETAAIAGVPQAQYFMGNAYRNGQGVEKNLALAIRWWTHAVE
                     FGQQRAAESLSQLRRQALSPNQSDRRRTDAIEAFRQYRDELWADYPNVVRNHSRESLG
                     IVLLKGSQVSHAVTMLLAEAFALSETAHDELARLYETGLDTSLAQFDTRILRCFAITA
                     ADGFIPSKKALARIYGKGLGVTQDVQKAKAMLKGLSKQEIQAVMDDIAE"
     gene            641..>1511
                     /locus_tag="VLL06_00835"
     CDS             641..>1511
                     /locus_tag="VLL06_00835"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HSS29541.1"
                     /translation="MLDASRLWRQFLSSSWLQAGAMGFIATVLAFSLSTAAAPTFTAL
                     DWALYDFWMADRSAIPVSPSLLIVTRDPASENEFGTGPWDRAIFARFLMSAHESGALA
                     IGLDHRLDHASPAQRGGAASDALLLESMSTAGPIVVVQSPDATLTSDTTIIGHLLVTT
                     HSDHIARMVPVAEIGDRESVPAFGIALYEAFQRQRSPTEPSRLERGTASILVNYVGNG
                     TLNAFPTVPMSAVWDAIEHHESDRLTEWFNGKVVVFLPSPMAGGGSLLPTGQAVTSSV
                     VHLHVLNMLLTDNR"
BASE COUNT          316 a          468 c          431 g          296 t
ORIGIN      
        1 acagtttatg gagaccgcgg cgatcgccgg agtcccgcag gcgcaatact ttatggggaa
       61 tgcctatcgc aacggacagg gtgtcgaaaa gaatctggca ctggccatcc gctggtggac
      121 ccacgccgtc gaattcggac agcagcgcgc agcagaatcc ctttcacagc ttcgccgcca
      181 agcgctatcc ccgaaccagt cagaccgccg tcgaacagac gcgatcgaag cgttcagaca
      241 gtatcgcgat gaactttggg ctgactaccc gaatgtcgtc aggaaccatt cgagggaatc
      301 actgggtatc gtcctactca aaggcagtca agtttctcac gcagtcacca tgttgttggc
      361 cgaagccttc gcgctgagcg aaacggcgca cgacgagctc gcgcgcctgt atgaaaccgg
      421 cctcgatacc agcctcgcgc agttcgacac acgcattctg agatgctttg ccatcactgc
      481 tgccgatggc ttcatcccgt caaagaaggc gctggcacgc atctatggta aaggtctcgg
      541 tgtcacgcag gatgtgcaga aagccaaagc gatgctgaag gggctatcca agcaagagat
      601 acaagccgtg atggatgaca ttgcggaata ggaaatagtc atgctcgatg cctcacgcct
      661 gtggcgacaa tttctcagct cctcctggct ccaagccggg gccatggggt tcattgcgac
      721 ggtcctggcc ttcagtctat cgactgcagc cgcacccacc ttcacagctc tggactgggc
      781 cctctacgat ttctggatgg ccgaccgttc agctatcccg gtcagcccat ccctgctcat
      841 tgtcacacgg gacccggcca gtgaaaacga gttcggcacc gggccatggg atagggccat
      901 ctttgcacgc ttcctgatga gcgcgcatga atccggagcc ctcgcgatcg gcctcgatca
      961 tcgactcgac catgccagcc cggcgcagcg cgggggagcg gcgagcgatg ccctgttgct
     1021 ggaatccatg agcaccgccg gccctatcgt cgtggtgcag agcccggacg cgacgctgac
     1081 ctcggacacc acaatcatcg gccatctgct ggtcaccacc cattccgatc acatcgcacg
     1141 aatggtgcct gtggcggaaa tcggtgaccg ggaatccgtc ccggcctttg gaattgcact
     1201 gtacgaggca tttcaacggc agcgctcacc gacggagccg tcacgtttgg aacgaggcac
     1261 ggcttcgatc ttagtcaact atgtcggcaa tggaaccctc aatgccttcc caacggttcc
     1321 gatgtctgct gtgtgggacg ccattgagca tcacgagagc gatcggctca ccgagtggtt
     1381 caacggcaag gtcgtcgtgt ttcttccaag cccgatggcg gggggaggct cgctcttgcc
     1441 cacgggacag gcggtcacca gctcggtggt ccatctccac gtcttgaaca tgctgctcac
     1501 cgataatcgg c
//
LOCUS       DATIPF010000034         3800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_888481, whole genome shotgun sequence.
ACCESSION   DATIPF010000034 DATIPF010000000
VERSION     DATIPF010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888481"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VLL06_00840"
     CDS             complement(<1..725)
                     /locus_tag="VLL06_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29542.1"
                     /translation="MSSPAQVLADFRSQVTQLLQERDKEWEASRKLVETKQLTTTLKR
                     LIEEAHHVDLPLIIRDAITLALGNSEAVRIQDLPGPRLKELTGLPPTKAVRALCVWFG
                     VIEGPKFQWPVTSLRSEEIEAFTHSHSNPFDLLQSADVASLLDLGSGDLSFATELVEQ
                     YVASLQQRQRELILHSLDRLQPGSKLGGPLHPEWERLNGLRSRTGLSFQFFGNRDMFD
                     LGGLDQTGKLAPRYTIATCWAPAT"
     gene            967..1884
                     /locus_tag="VLL06_00845"
     CDS             967..1884
                     /locus_tag="VLL06_00845"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HSS29543.1"
                     /translation="MGTKSSLVSQDGNHVPFTEWQVIDTATEQPVPLDQWISLLLQQD
                     IIYLGEEHHNRFHIDAALTVLRRLKAEGRIPALGMEMFGWDGQAALDQYIFGSDMNRQ
                     DFLQAVKWQQNWGGSYEDYEPLVRLAKEQHWTVDAMNPPKPLVRIVAKNGLAQAESDP
                     IMAQWGMKDETLVDDPIYRTRILQQLQACHGGGADSLYQTMFEASMVRDEGMAKTLVH
                     RLNQIRSGSDMMAGPVVSYTGAGHIQYNLPVPKRVARRLTGQIRQVSVYMTSFDVRRI
                     EELQDMIREKIADYLWLTPVSEKGLPRRC"
     gene            2216..3052
                     /locus_tag="VLL06_00850"
     CDS             2216..3052
                     /locus_tag="VLL06_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29544.1"
                     /translation="MVDRNAFHIGNQYLAENRVRIVEADDAQISSAVIGNSGLYEQTI
                     RLKEGHLISKCSCALPEEPMCRHCIAVLLEYHRWAQPRNGQQKKVKPSTAPQPAQPTP
                     SSAGASASSSTSTTDLKLGEVMAFVEWLHPAIKALERGQTLPDSPKPSGDLAPWIQAI
                     RNLEERRRESEEARMNLEADMRDREAYVGRLTQQVQTSMDEVKVSQANAQQLQQELTL
                     CKDMLAKVSEIASEVWNYDSQLKSLAGEVMSKGSQLDKLAHSFKEVSTALKALGKTST
                     TP"
     gene            complement(3534..>3800)
                     /locus_tag="VLL06_00855"
     CDS             complement(3534..>3800)
                     /locus_tag="VLL06_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29545.1"
                     /translation="LRDAVYWSPSLTLAWLIAQSLRTSLTEMESFRQCPFPKRTKALR
                     AGRPRLLGQTMNVSSRVRLTGNGTARQTEGSNQGTLVPQTSIEG"
BASE COUNT          917 a          963 c         1085 g          835 t
ORIGIN      
        1 gtcgcgggag cccagcaggt ggcgatcgta tagcgaggag cgagtttccc agtctggtcg
       61 agacctccca aatcaaacat gtcccggttt ccaaagaatt gaaacgaaag gccggtccga
      121 gatcgaaggc cgttgagtcg ctcccattcc ggatgaagtg ggccacccag ttttgagcct
      181 ggctgaagcc gatcgagact atgaagaatc aattcgcgct gtcgctgttg aaggctggct
      241 acgtattgct ctacaagttc tgtggcgaac gagaggtcgc cagaccccag atcgagcagg
      301 gaggccacat ctgcactctg cagcaggtcg aagggattgg aatgggagtg ggtgaacgcc
      361 tcgatctctt cgctccgaag ggaagtgact ggccactgga acttcgggcc ctcaatgacg
      421 ccaaaccaca cacacagagc ccgaacggct tttgtcgggg ggagccctgt gagctctttc
      481 agccgggggc cggggagatc ctgaattcgt acggcttctg aatttcccaa cgccagggtg
      541 atcgcatctc ggatgatgag gggaagatcg acgtgatggg cttcttcgat caaccgtttt
      601 aacgtcgtcg tgagttgttt tgtttcaaca agtttacgtg aggcctccca ctccttgtcg
      661 cgttcctgca agagctgggt tacctgtgaa cgaaagtctg caagaacttg tgccggagaa
      721 ctcataagtc ggctagcgcg ggacgcgtat agttgatcag tcgatgtcag ttggtgaatg
      781 tgtagcagca taacgaccta gtgcatgagc gtcaaggttg gcgtcttgcc gatctcgaga
      841 cacctgtgtt aggtagaggt catgcatggg acaccggtga tcatcgctag acgagcaatt
      901 cttgtcatac agagagtgtt gttggtcatt ggagcactgt gcttcaccat agcctgcacg
      961 agtggagtgg gcaccaaatc ttctctggtc tcacaggatg ggaatcatgt gccgttcacg
     1021 gaatggcagg tcattgatac cgcaacggaa cagccggtcc ctcttgacca atggatatca
     1081 ctgttgctcc agcaagacat catttatcta ggcgaggaac accacaatcg gtttcatatc
     1141 gatgcagcac tgacagtatt gcggagactg aaggcagaag ggcgtatacc ggctttgggt
     1201 atggaaatgt tcggctggga cggccaagcg gcactggatc agtatatttt cggttcagat
     1261 atgaaccgcc aggatttcct tcaggccgtc aaatggcaac aaaattgggg tgggtcgtac
     1321 gaagactatg aaccgctagt tcgattggca aaagaacagc attggactgt ggatgccatg
     1381 aatcctccaa aaccgttggt gaggatcgtc gccaagaatg gcttggctca ggcggagtct
     1441 gacccgataa tggcccaatg gggtatgaag gacgaaaccc ttgtcgatga tccgatctat
     1501 cggacgcgta ttcttcaaca actccaggct tgccatggtg ggggagctga cagcctctat
     1561 caaacgatgt tcgaagcctc gatggttcga gatgaaggga tggccaagac attggtccat
     1621 cgccttaacc agattcgctc ggggagcgat atgatggctg ggccggtggt gagctatacg
     1681 ggggctggac acattcagta caaccttccg gttccaaagc gagtggcccg acgcttgacc
     1741 ggtcagattc gtcaggtcag tgtctatatg acgtcgttcg atgtgagacg cattgaggaa
     1801 ttacaagaca tgattcgcga gaagattgcg gactacctat ggctaacacc ggtgagtgaa
     1861 aaaggactgc ctcgccgatg ttgagaaagc cctgccaacg gaggaattct ttcaagagaa
     1921 acgacgagta ccactctgcg ggatgctatg gctgtcggca gacaaaaaat ctcccatctc
     1981 ggttgagctc cactctagaa cctgatcagg tggcgctggc tgcgaatccg cacagtaagg
     2041 gtgatgcaac cgtccgttga aaaccgatga gcccttgaca gattcccatg tctgtttcag
     2101 actatggagg aagaccagtt ggagttgtgt gcgattggga acacagcgca tgcgaaataa
     2161 gatcccttgc tccgccgagc ggttaagcct tttggatcct ccaatgatcc aagcggtggt
     2221 tgatcgcaat gcctttcaca ttggtaatca gtacctcgct gagaatcgcg ttcggattgt
     2281 cgaagctgat gacgcccaga tttcgtccgc cgtcatcggg aactccggcc tctacgaaca
     2341 gacgatccgt ctcaaggaag ggcacctgat ctcgaagtgc agttgtgcac tgccggaaga
     2401 gccgatgtgt cgtcactgta tcgcggttct actggaatac caccgctggg cacagccaag
     2461 aaatggccaa cagaaaaagg taaagccatc cacagcccca cagccggcac agcccactcc
     2521 gagtagcgcg ggcgcatctg cgtcaagctc cacttcgacg actgacctga agctgggtga
     2581 ggtgatggcc tttgtcgaat ggctgcaccc tgcgatcaag gcgttggagc gtgggcaaac
     2641 gttgcctgat tcgccaaagc ccagcggcga tctcgctcct tggattcagg ccattcgcaa
     2701 tctggaagag cgacgccgtg agagcgaaga agcacggatg aatctggaag ccgacatgcg
     2761 ggaccgagaa gcctatgttg gccgactgac gcaacaggtg caaacctcca tggatgaggt
     2821 caaggtttcc caagccaatg ctcagcaact gcagcaggaa ctgactctgt gcaaagacat
     2881 gctcgcgaag gtctccgaga ttgcctcaga agtctggaac tatgacagtc aactcaagtc
     2941 tcttgcaggt gaggtgatgt caaagggatc acaactcgac aaactcgcgc attcttttaa
     3001 agaagtctcg acagcattga aagcgctggg caagacctct accacccctt agtctctccc
     3061 gattagtttt acctcgcaga ctctatccct atttcagaag ctcgtgtgtc ctatcaattg
     3121 gcttccatgt acgccactct cgtgtggtcc aattgattcg agggaagatt gagtcataac
     3181 atggagtgag gcttcgactg cccagattgt tccatgcacg gcggctggcc cctcatccat
     3241 caggaggtcc gatatcgtag gaaacgagga agagcccgac tagtcttcac ctacgacggc
     3301 acctcacgga acggcgagta tccggtcacg caaaggccac tatgcccaac aagatagcgc
     3361 taggctatta agatggttgg cgtcgttatc gaccatttac atcggtcaga ggagcagttt
     3421 gatgattcat caatgcctat tcagaagaga agtaggcacg gcggctcgga accgcaaatc
     3481 caagaaacag tgcggatctg ctctggttaa tgagagaact acctgctcgt gtgtcatccc
     3541 tcgatggacg tttgtggaac aagggtgccc tgattgctcc cctcagtttg acgggctgtt
     3601 ccattgccgg tgagcctgac acgagaggac acgttcatgg tctgaccgag caatcgaggc
     3661 cgtcctgcgc ggagcgcttt cgtccgtttt gggaaaggac actgccggaa ggactccatc
     3721 tcggtcaagg aagtccgtag ggactgagca atcagccatg cgagcgtcaa actagggctc
     3781 cagtatacgg catcgcggag
//
LOCUS       DATIPF010000035         5928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_977124, whole genome shotgun sequence.
ACCESSION   DATIPF010000035 DATIPF010000000
VERSION     DATIPF010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977124"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VLL06_00860"
     CDS             complement(<1..291)
                     /locus_tag="VLL06_00860"
                     /inference="COORDINATES: protein motif:HMM:NF015980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29546.1"
                     /translation="MKAPQFGSWRTIQAQALVAGQVTAQPDGRMRVDFRLWDVFGESQ
                     MIGQQYFATPDNWRRIAHIISDAIYERITGEKGYFDTRIVFVAESGPAAKRVK"
     gene            complement(440..1036)
                     /gene="yihA"
                     /locus_tag="VLL06_00865"
     CDS             complement(440..1036)
                     /gene="yihA"
                     /locus_tag="VLL06_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HSS29547.1"
                     /translation="MRILTAEFIRSCAEPEQFLKGELPEVAFIGRSNVGKSSLINSLL
                     QRKGLAKVSRTPGKTRLVNLFRITSDDPGLSRFVVVDLPGYGYAKVSKALRAQWAPLI
                     EQYLDGSEQLRAIVVLVESRVLSEQDSQTITWLSSVGRPPVVVATKVDKLKMSERVGA
                     LRRLQEGLGLVEEVIPYSSVTGEGRDRLWMVLKERIRT"
     gene            1111..1857
                     /gene="mtgA"
                     /locus_tag="VLL06_00870"
     CDS             1111..1857
                     /gene="mtgA"
                     /locus_tag="VLL06_00870"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HSS29548.1"
                     /translation="MRKSTARSRLKVYLIIGLCLPVGAVLFFWLLAMPNISALRTTNP
                     PVTALMEGRQALAKAQGHTVERHWTWVPLSRISPYLRQAVVAAEDASFFTHEGFDWEG
                     IKDAALYNLEAGELKRGGSTITQQLAKNLYLSSERSLLRKARETLITRSLEQQLTKER
                     ILELYLNIAEWGKGVYGAEAAARHHFKKSARTLTSDEAAWLAAILPSPRRYDPLRKTT
                     ALARRHERILSRIDREPTPPEPSDPEPSEQ"
     gene            complement(1872..2321)
                     /locus_tag="VLL06_00875"
     CDS             complement(1872..2321)
                     /locus_tag="VLL06_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSS29549.1"
                     /translation="MKDVLFHLAFPTHDVEAAKRFYVDGLGCTLGRESSHAVTFGLAG
                     HHLVAHLTTELPKRQQGIYPHHFGLVFLSKDSWEQFLAQAQANNLSFYQQPRVRFPGT
                     RIEHHTFFLEDPSGNLLELKHYTHESAILGERDVHAVGDSSESPEEP"
     gene            2700..2939
                     /locus_tag="VLL06_00880"
     CDS             2700..2939
                     /locus_tag="VLL06_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29550.1"
                     /translation="MPNVLHPFRHKNPRARIPETPFPEPYIFNDTENIFVDDILALFN
                     KNGLMLHQSIDLLERSGRSERIDEMEQRLAKRTTD"
     gene            3102..3749
                     /locus_tag="VLL06_00885"
     CDS             3102..3749
                     /locus_tag="VLL06_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29551.1"
                     /translation="MGSPTIAYLVTARHVLVNDTKALVKPFALVRGNASNPNEDRGYV
                     LELDLDVLQRSGNWKEDLEHDVLVVRFMWDSKLMEGVQVKYTPPSGPAMVPLPSVLRL
                     HDITLQKAVYLAGYRVSLIPRGAETSELNIPKIREGKALLKDVQKGQIVSDYIAVGGD
                     SGAPVFIFDGDASGGAPKLIGIHIGRQDCVTPEGPFKCAIATPMDFVFALISTFK"
     gene            complement(3891..4457)
                     /locus_tag="VLL06_00890"
     CDS             complement(3891..4457)
                     /locus_tag="VLL06_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29552.1"
                     /translation="MGTQVPPRHPLRQLFGALTEKSFIEHLGWPDPNVTGYLSNLLVE
                     FTHTDQLYKIRNQQDQPIDAVVDLLFESEALLEAQSMERERDVHQHIGDFTLFMAGLF
                     PEYLRRLKTAGLIYHKDFLVDYMKTGKRSYGIVARMADGPSQEEPPLFRKLSDNFELC
                     VTGLGFVRSDLDRMKDPTYRQARNLLLN"
     gene            complement(4755..5204)
                     /gene="dtd"
                     /locus_tag="VLL06_00895"
     CDS             complement(4755..5204)
                     /gene="dtd"
                     /locus_tag="VLL06_00895"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HSS29553.1"
                     /translation="MKAVIQRVTKASVHVEGKTVGQIGSGLLVLLGVAKDDGETDSRY
                     LVEKIRTLRIFSDDQGKMNRSLVDVEGSVLLVSQFTLLGRIAHGRRPSFDEAASPDEA
                     KRLYEQVVTGLQHQGTHVETGVFAADMQVELLNDGPVTFVLDSHGGS"
     gene            complement(5208..5732)
                     /locus_tag="VLL06_00900"
     CDS             complement(5208..5732)
                     /locus_tag="VLL06_00900"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HSS29554.1"
                     /translation="MRQAIIFKSRWGWVGISETTKGIDAVVLPKGSRQAVLTELRLSD
                     AELLEGRMSSRLRKAQTQLTDYLIGVRKSFDLPLDLSRGTSFQRKVWRTLRRVSYGRL
                     RSYRWLAVRVGGRQYARAVGNAVGANPMPIVIPCHRIVAHDASLGGFSGGLPTKRKLL
                     TLEGTLAELRPGRS"
     gene            complement(5741..>5928)
                     /locus_tag="VLL06_00905"
     CDS             complement(5741..>5928)
                     /locus_tag="VLL06_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29555.1"
                     /translation="WVAAAQVWTHPGEWIEKSLMLAMGIGLSVGGYLGVHALLRSEEL
                     DLVWGMVRKKLGRVMGR"
BASE COUNT         1321 a         1786 c         1524 g         1297 t
ORIGIN      
        1 cttcacgcgc ttggcagccg ggcccgactc cgcaacgaag acgatgcggg tgtcgaagta
       61 gcccttctcg ccggtgatgc gctcatagat ggcatccgag atgatgtggg cgatgcggcg
      121 ccaattgtcg ggcgtggcga agtactgctg gccgatcatc tggctctcgc cgaacacatc
      181 ccacaggcgg aagtcgacgc gcatgcggcc gtcgggctgc gccgtgacct ggccggcgac
      241 gagggcctgc gcctggatgg tgcgccagct gccgaattgc ggcgctttca catcatgcga
      301 ctggaggaca agaaacccaa acagttccgc ccgtatgagg aagtccgcac agaaatccag
      361 ggactcgtct ttcagcagaa aaacgaggat gtcttccagt catggctggc ggagctcaaa
      421 aacaaggcct acatcgagat caagttcgaa tccgctcttt caggaccatc cacaaccggt
      481 cccgtccctc gcccgtcacc gacgagtaag gaataacctc ctcgaccaat cccagccctt
      541 cctgtagacg acgaagcgca ccgacgcgct cgctcatttt taatttatcc acttttgttg
      601 ccaccacgac ggggggacgt cctacggacg agagccaggt gatcgtctga ctatcttgtt
      661 cactcagtac ccgactttcg accaagacca ctatggcgcg caactgctca ctcccatcga
      721 gatactgctc gatcaatggc gcccactgag cccgcagcgc tttcgacacc ttcgcgtatc
      781 cataccccgg caaatccacc accacgaatc gcgatagccc gggatcgtca ctggtgatcc
      841 ggaagaggtt gaccaagcgg gtctttcccg gcgtacggct caccttggcc agtcctttgc
      901 gttgcaagag ggaattgatg agagaggact tgccgacgtt ggaccgcccg atgaaggcga
      961 cttccggaag ttcgcctttc agaaactgtt ctggctccgc acaacttctg ataaactctg
     1021 cggtaaggat tctcatgcaa cacccatcca ccagcccggc accatgatat tgacgcaaga
     1081 acctatcgcc tctgtgatac gcagcctctg atgcggaagt caaccgcacg atcccgcctg
     1141 aaagtctacc tgatcatcgg cctttgcctt ccggttggag ccgtcctctt cttctggttg
     1201 ctcgcgatgc ccaacatctc ggccttacgc acgacgaatc cacccgtgac agccctgatg
     1261 gaaggccgac aggctctggc taaggcacaa ggccacaccg tcgaacgcca ctggacatgg
     1321 gtgccgctct cacgcatctc cccctacctc cgccaggcgg ttgtggcagc ggaagatgcg
     1381 tcgttcttta cccacgaagg attcgactgg gaagggatta aggatgcggc tctgtataac
     1441 ctcgaagcgg gcgaactcaa gcgtggggga agtaccatta cacagcagct ggccaaaaac
     1501 ctctacctgt catctgaacg ttccctgctt cgaaaggcgc gggaaactct cattacgcgc
     1561 tccctcgaac agcagctcac gaaggaacgt attctcgagt tgtacctcaa catcgcagag
     1621 tggggaaagg gtgtctatgg agctgaggcc gcagcccgcc atcacttcaa gaagtccgcg
     1681 cgtaccctga cgtccgacga agccgcatgg ctcgcggcca tcttgccgtc gccgcgccga
     1741 tacgatccgc taagaaaaac gaccgccttg gctcgccgtc acgaacgtat cctgagccgg
     1801 attgatcggg aacccacccc tccggaaccc tcagatccag agccctcaga acagtaattg
     1861 agtctggcag attatggttc ctctggactt tcagaggagt cgccgaccgc gtggacatcc
     1921 cgctcgccca aaatcgccga ctcatgggta tagtgtttga gttcgaggag atttcctgag
     1981 ggatcttcga ggaaaaacgt gtgatgctcg atgcgcgttc caggaaaccg cacgcggggc
     2041 tgttgataga aggacagatt gttcgcctga gcctgcgcca agaactgttc ccacgagtct
     2101 ttcgacaaga agacaaggcc aaaatggtgg ggataaatgc cttgttgccg tttcgggagt
     2161 tccgtcgtca gatgcgccac aagatgatgc cctgcaagtc cgaatgtgac ggcgtggctc
     2221 gattcccgac ccaacgtaca accaagtcca tcgacataga agcgtttcgc cgcctccaca
     2281 tcatgcgtgg gaaaagccag atgaaagagc acgtccttca tggaaactgt cctcgatcag
     2341 tcggctggcc ggtccccttc cctcagccgg cagtcatttg tggtgcgtca cgactaacgg
     2401 tcactccctc agcttagtct tcttgagggg cgcccttcaa gtcggtctcc catgtggaat
     2461 ggctcgacgg catgttgggt agccattcag gcagacctca ccttctcgcg tgaaggtgag
     2521 gtgatctcga aattacggac gcctggatga gaactcattg atcagctgct cgaactcctt
     2581 ggaccgagta gccggcaggg atacgacgtg aagcttcgat cgcatcattt cccctgtcag
     2641 cccttgcgcc ttccttcata accctcttag aatgcacctc cttccttgcg agcctcccta
     2701 tgcctaatgt cctgcatccc ttccgccaca aaaaccctcg cgcgcgtatc cctgagaccc
     2761 ccttccctga gccctacatt ttcaacgaca cggagaacat attcgtcgat gatatcttgg
     2821 cgttgttcaa caagaacggg ctgatgttgc accagtccat cgacttgctc gaaaggtcag
     2881 gaaggtctga gcggattgat gagatggaac agcggctggc gaagaggacg acggactgaa
     2941 tgaattattt tctacctgtt cacgccgctc tcctctttat tctcctacag ataggctttc
     3001 agccgctcct tcacgcctcc tccaatacgg atactcttct tcactaccca gtcattgttg
     3061 cacctggtgc aatttttggg cgaacagcgt caggagtatt tttgggctca cccaccatcg
     3121 cgtatttggt cacagcccga catgttctag tgaacgacac aaaagcgctg gtcaagcctt
     3181 tcgcgctagt tcgaggaaat gcgtccaacc caaacgagga tcgcggctat gtgctggaac
     3241 tggatcttga tgtacttcaa cggtccggta actggaaaga ggaccttgag cacgatgtcc
     3301 tcgtcgttcg attcatgtgg gactcgaaac tcatggaagg ggtgcaggtg aagtatacac
     3361 caccgtcggg accagccatg gtgcccctcc cctctgtact gcgcctgcat gacatcacct
     3421 tgcagaaggc cgtgtaccta gctggatatc gggtttcact catcccgaga ggggcagaga
     3481 cgtccgaact gaatattcca aagatcagag agggaaaggc gcttctcaaa gatgtgcaga
     3541 aaggccagat tgtgtctgac tacattgctg ttggcggcga cagtggcgcg ccagtcttca
     3601 tattcgatgg tgacgcgagt ggaggggccc ccaagttaat cggtatacat atcggccgac
     3661 aggattgcgt aacccctgaa ggtccattta aatgtgcgat tgcgactccg atggattttg
     3721 tctttgcact catctcgaca ttcaagtagc accatcacca cgctccacgg ttgtagagtt
     3781 gtcgatacgg tgtcgagtcg taactggtcg atacctgaat gaagcacgaa cgccattggt
     3841 cccgcaatcc gacatttcgc ggcgcgtcca gagaagcggg agttggtgag tcagttgagg
     3901 agaagatttc gagcctgccg gtacgtcgga tctttcatgc ggtcaagatc ggaccgcacg
     3961 aaccctagcc cagtgacaca gagctcgaag ttgtcggaaa gctttctgaa cagaggcggt
     4021 tcctcctgtg acggaccatc agccattcgc gcgacaatcc cgtacgagcg ctttcccgtc
     4081 ttcatatagt ccactaggaa atctttgtgg tagatcaatc ccgccgtctt gaggcgtcga
     4141 agatattccg gaaacaatcc tgccatgaag agcgtgaagt cgccgatgtg ctgatgcacg
     4201 tctcgttcac gctccatcga ctgggcttcg agtaatgctt ctgattcgaa caagagatcc
     4261 accactgcat cgatcggttg gtcttgctga tttctgattt tatacagttg gtcggtgtgg
     4321 gtgaattcga cgagtaggtt tgacagatag ccggtgacgt tggggtctgg ccacccaaga
     4381 tgttcgataa agctcttctc ggtcagagcc ccgaacagct gacggagcgg atgtcgtggc
     4441 ggaacttgcg ttcccatatc cacctctcac acattccgaa aacctatgaa ttcagtatac
     4501 cagaacaatg gtcgacatgc cgatgtcggc cctctgatcc ttctatcggt cttcacctac
     4561 gagacttgag catgggcgga actgcgaagc caacaagctg atcaaaaagc tggcctcttt
     4621 ggatctcgtc cgttttgact atccagttcc cattgtgaat ccggttcgtg ttgttcgacg
     4681 atgatagacg ggacagacca atacattttg gcctacccca cagaatgttt gcctatcttg
     4741 cttgtcactg tctactagct cccaccatgg ctatcgagta caaaggtcac cggtccgtcg
     4801 ttcagcaact cgacttgcat gtctgcggca aagacccctg tctccacgtg agtcccttgg
     4861 tgttgtaacc ctgtcacaac ctgttcgtat aatcgcttgg cttcatccgg cgacgccgcc
     4921 tcatcaaagc tcggacgacg gccatgtgca atcctaccca gtaacgtaaa ctgggaaaca
     4981 agcaagactg atccctctac gtccaccaac gagcggttca tcttcccctg gtcatcggaa
     5041 aagatgcgaa gcgtgcgaat tttctcgacc agataccggc tatccgtctc tccatcgtcc
     5101 ttcgctacac ccaacagcac gaggagacct gatccaatct gccccacggt cttgccttcg
     5161 acgtggacag acgctttcgt cactcgctga atcacggctt tcatgagtca cgatcgtccc
     5221 ggtcgcagct cagccaaagt tccttcgagg gtgagcaact ttcgtttcgt gggcaagccg
     5281 cctgagaacc cgccgaggga tgcatcgtgt gccacaattc gatggcacgg aatcacgatc
     5341 ggcatcggat tcgctcccac ggcattgccg accgcacggg catactgtct acccccgacc
     5401 cgaacagcta gccatcgata ggagcgaaga cggccgtagg aaacacgtcg aagagtccgc
     5461 cagaccttcc gctgaaaact cgtcccccgt gagaggtcca acggcagatc gaaggacttc
     5521 cgcaccccaa tcagatagtc agtcaattgg gtctgtgcct ttcgcagtcg cgaagacatc
     5581 cggccctcca gcagctcggc gtcggacaat cgcaactctg tcagcacagc ctgacgcgac
     5641 cccttcggca acaccaccgc atcgatccct ttcgtcgttt cggaaatacc cacccagccc
     5701 catcgcgatt tgaaaatgat cgcttgtcgc attcgtgacc tcagcggccc atgactcggc
     5761 ccaatttctt ccgcaccatt ccccacacca agtcgagctc ttctgaacgc agcagggcgt
     5821 ggacgcccag atacccgccc acgctcaatc caatccccat agccagcatg agcgacttct
     5881 cgatccattc accgggatgt gtccacacct gcgccgccgc cacccagg
//
LOCUS       DATIPF010000036         4944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1065383, whole genome shotgun sequence.
ACCESSION   DATIPF010000036 DATIPF010000000
VERSION     DATIPF010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065383"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="VLL06_00910"
     CDS             complement(<1..1315)
                     /locus_tag="VLL06_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSS29556.1"
                     /translation="MLVKRWLVGDPLKSAQAAHQRLSKTIALAIFSSNAISSVAYATE
                     EILLVLVLAGTAAIAWSIPISLSILLLIVILTISYRQIIHEYPEGGGAYVVARANLGE
                     QPALIGAAALMIDYVLTVAVSVAAGIAALTSAVPGFFAYREALGLAATVFIIFMNLRG
                     VQESGKFFAFPTYFGIGILGMMVGIGSIQAFFGEGASPSADHQTAVEDLTLFLVLRAI
                     AAGCTAVTGMEVISNGVKTFRQPESQNAATTMLYMSLILGSLFIGISVLASHFGIQPK
                     LDETVVSQLARLTFGTGALYYALQLGTMLLLVLAANSAFAAFPQLASILARDRYMPRQ
                     MATFGDRLVFSNGIIILGIIACLLLIIFHGDPHALIPLYAVGVFISFTLSQAGMVRRW
                     LTKKGPHWQMKLMVNGLGSVSTGIAIIIIATTKFTQGAWIVFLLIA"
     gene            1963..2757
                     /locus_tag="VLL06_00915"
     CDS             1963..2757
                     /locus_tag="VLL06_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29557.1"
                     /translation="MKSALSIVFGVAAVAFVAAPLTSFAGGTISGKVTYAGKAEQKEF
                     LFSKFPNPKFCPQNPHKDLMDGDKRFLKTIEVGKDGGLKHAVVAVIDVEDKAFMDGYQ
                     GTEVVAEFCEFLPFAGIVVNNKSFKVENHDADPDDPKSVQGVLHNPHSFVVKGTSSAT
                     GFNIGLAKKGDKLDKPVVFRGGAEKDGFYRLQCDQHEFMQGFFLPVSSPHFAVVKDDG
                     SFEIKDVPAGKHKVVAWHPFAAKGKKVEFEVDVADGGTANLKAEIK"
     gene            3050..4039
                     /locus_tag="VLL06_00920"
     CDS             3050..4039
                     /locus_tag="VLL06_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HSS29558.1"
                     /translation="MSRELSLAEIFQLGYYWETKILLTAVRLDVFSALDGKQKTLYEV
                     ADRLGVHRETLGLLLNALVAMQLLRKDGDSYGNSTVSATYLVRNSAQYVGHLLLLHDA
                     EWDNWGKLEQTIRTGQRLVDRHVFETDPDLGSNVLAVLHRIGQQSGPDLARRLQLSGP
                     VRFLDLGGGAGTNAIAFCQAYPELTATVFDLPATLQLTERMVKEAGLESRITLRPGDF
                     NKDGLGGPYDAVLMSDILHYQDLSTNAVLVKRVWDHLAPGGRLVIKDRFLNETGTGPA
                     WTTAFAVHILVNTERGGCYKTADAVQWMGEAGFQTVTELEPSAVVQGIRAREV"
     gene            4097..4813
                     /locus_tag="VLL06_00925"
     CDS             4097..4813
                     /locus_tag="VLL06_00925"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HSS29559.1"
                     /translation="MFEWLREPGFFGTHATVGADISQLMATLFTGLFVIGWFQARRHR
                     ADVHHWMMFGGMIAMLAFFISYYLFRQLGVLAFEGKEGFGGSEALYHNVFIPILIVHI
                     VLVVVGLVMAVYMIVLGFRAQLIEQGRRILSDRVLQTTWGKIGMIFGIVCLPVALYLL
                     YLMAVDRLRMGRLVVWAGFLAIIAVVFAVEMTIQRLWPDGGHRHRALGRFTMAIYCIL
                     FVTGTTTYTMLYLLYPGKIG"
BASE COUNT         1136 a         1175 c         1516 g         1117 t
ORIGIN      
        1 gggcaatgag gagaaagacg atccacgcgc cctgtgtaaa cttggtcgtg gcaatgatga
       61 tgatggcaat gccagtcgag acagagccta ggccgttcac catcagtttc atttgccagt
      121 gaggcccctt cttggtcagc catcggcgca ccatgccggc ttgagataac gtgaaggaga
      181 tgaacacgcc gactgcatag agcgggatca gggcatgtgg gtcgccgtga aagataatga
      241 ggagcagaca tgcgatgatc ccgagaataa tgatcccgtt cgagaacacc aagcggtcgc
      301 cgaacgttgc catctggcga ggcatatagc ggtcgcgtgc aaggatcgac gcgagttggg
      361 ggaaggcggc aaaggcgctg ttggctgcaa ggactaacag gagcatcgtc ccaagctgga
      421 gggcatagta gagagcccca gttccgaagg tgagacgcgc caattgagac acgaccgtct
      481 catccagctt gggctggatc ccaaaatgag aggccaggac actgatgccg ataaataacg
      541 atcccagaat cagtgacatg taaagcatag tggtcgcggc attctgggac tccggctgcc
      601 gaaaagtttt tacgccgttc gaaatcactt ccattcctgt cacagcagta cagccggcgg
      661 caattgctcg aaggaccaag aagagagtca agtcttcgac cgcagtttgg tggtctgcag
      721 atggcgaagc accttctccg aagaaagcct gaatgcttcc gatccccacc atcatgccga
      781 ggatgccgat gccgaagtag gtagggaacg cgaaaaattt ccccgattcc tgcacgccgc
      841 gaagattcat aaagataatg aacacagtcg cagcgagccc cagggcctcg cgatacgcga
      901 agaaacccgg tacggcggag gttagtgccg cgatgcctgc cgccacgctg actgctaccg
      961 tgagaacgta gtcgatcatc aatgccgccg cgccgattaa cgccggctgt tcccccaagt
     1021 tggcacgggc gacaacatat gccccgcccc cctccgggta ttcgtggatg atttggcgat
     1081 aggaaatggt caggatgacg atgagaagca ggatcgaaag gctgatggga atggaccagg
     1141 caatggcggc agttccagcg agcaccagaa ccagcaagat ctcttccgtg gcataggcta
     1201 cggaagaaat cgcgttcgaa gaaaagatcg cgagagcgat ggttttcgac aaccgttggt
     1261 gtgctgcctg ggcgcttttg agtgggtcgc caaccaacca acgcttcaca agcataacga
     1321 tcattatcgc atagaagtgc agacgtgcac atcgaagaag cagttgtgag gtgtcagagg
     1381 ctagacgcct cacccgtctg tgcggtaagg gaatccgcgt gagaggaaac gcgagtggtg
     1441 tcagtggtca aacaggatca tggaaagatt gccagccggt gtgaagacca atggctgtgg
     1501 tgaagatgtt gaagaagcac gtcccatctc ttcatgttgt gggatgtggg tgtgagcgtg
     1561 aatcgttgtt cgctctggaa gtttcggaag aagtatcttg gatattagat ggatagcgtc
     1621 caaggtcaat gtgccgggag cgatttctct gctcgcttat gaaggaattc ctttcacaat
     1681 acggatgttg ccgtgtgggc tagatccagg gggccgcttc atcttgttct gttcaagaag
     1741 taggcgttga atgggctttt gcaccctctt gtttcagatt gacaggatac ggtaggattt
     1801 tatatacata ctcggtccaa gaccagcctc gccagcaagt tgtcgatcga gcacaccaga
     1861 gaggaagccg ttcccatatt tctttcatcg ggcaggtgga tgaaggaagg gaatggggag
     1921 ctcagaatta gggcctatcg ttaatcacaa ggaggtagtc acatgaagag tgccttatca
     1981 atcgtatttg gcgtggcagc cgtcgcgttc gtcgcggcgc ctctcacctc gtttgccggc
     2041 ggaacgattt ccggcaaggt gacctatgcg ggaaaggcag agcaaaagga atttttgttc
     2101 tcgaagttcc ccaatcccaa gttttgcccc cagaatccac acaaagattt gatggatggg
     2161 gataagcggt ttttgaagac catcgaagtc ggcaaggatg gcggtctaaa gcatgcggtg
     2221 gtggccgtca ttgatgtcga agacaaggcc ttcatggatg ggtatcaggg cactgaggtg
     2281 gtggcggaat tctgcgagtt tctgccgttt gccggtattg tggtgaataa caagtccttc
     2341 aaggtggaga accatgacgc cgatccggac gatcccaagt cggtccaagg tgtgctccac
     2401 aatccgcaca gctttgtggt gaagggcacc agctcggcca ccggtttcaa tatcgggttg
     2461 gccaagaagg gtgacaagct cgacaagcca gtggtgttcc gtggcggagc ggagaaggat
     2521 ggcttctatc gtttgcagtg cgaccagcat gagttcatgc agggtttctt tctcccggtc
     2581 tcaagcccac actttgcggt tgtgaaggac gatggttcct tcgagatcaa ggacgtgccg
     2641 gccggcaagc acaaggtggt ggcctggcat ccgttcgccg ccaagggaaa gaaagtggag
     2701 tttgaagttg atgtggccga tggcgggact gccaacctca aggccgaaat caagtaagct
     2761 gacgtttcag attggcgggg tcacccgtac agaaagggca gcggagcaat ccgctgccct
     2821 ttctgtttga cgtgcctgca tgggcactca ctcgccagca tcactcgtca caaaatcgcc
     2881 gagacggcaa gcgagacgga tatgcgaagc tgtcatagat ggcagtagag tagcggatag
     2941 taccctcttt tcgattgcgg tgtgtcacat cgaatcagct gccttgcctt tcagtttccg
     3001 atctaggtaa gatgccgagt ttcaaagcta tttgcttgaa gggagtggtg tgtcacgaga
     3061 actctcgctc gcagaaatct tccagcttgg gtattactgg gagaccaaaa ttctgctgac
     3121 ggccgtcagg ctggatgtgt tttctgcctt ggatgggaag caaaaaaccc tctacgaagt
     3181 ggccgatcgt ctcggcgtcc atagggagac gctgggattg ctgttgaatg cccttgttgc
     3241 catgcaacta ttgaggaaag acggggattc ctatgggaat tcgacggtct cggccaccta
     3301 tctggtccgc aattccgctc aatatgtcgg gcatctcctg ctgcttcatg atgcggaatg
     3361 ggacaactgg ggcaaattgg agcagacgat tcgtacagga caacggttgg ttgaccggca
     3421 tgtctttgaa accgaccctg acctaggtag caacgtgctg gcagtgctcc atcggattgg
     3481 acaacagagc gggccggatc ttgccagacg gctgcagctg agtgggccgg ttcgattcct
     3541 ggatttggga ggcggagccg ggaccaatgc cattgcgttc tgccaggcct atccagagct
     3601 cactgcgacg gtgttcgatt tacctgcgac gctccagctg acggaacgaa tggtgaaaga
     3661 ggctggcttg gaatcgagga ttacattgcg ccccggcgat tttaacaagg atggactagg
     3721 cggaccctac gacgctgttc tgatgtccga cattctgcac tatcaggacc tttcgaccaa
     3781 cgcggtattg gtgaagaggg tctgggatca tttggcgccc ggcggccgat tggtcatcaa
     3841 ggatcgattt ttaaatgaga ctggaaccgg tccggcctgg accacggcgt ttgcggtcca
     3901 tattctcgta aacactgaac gaggtggctg ttacaagacg gcagatgcgg ttcagtggat
     3961 gggggaagcc ggctttcaaa ccgtgacaga gttggaacca tcagcggtcg tgcagggcat
     4021 aagggctcgt gaagtgtgat gcgtctctta tgaagcgcat tgtgagagca acgagagacg
     4081 aacgacgagg agatccatgt ttgaatggct gagagagccg ggatttttcg gaactcacgc
     4141 cacagttggg gcggatatca gccagctcat ggcgacgctc ttcacggggc tcttcgtcat
     4201 cggctggttt caggcacgga gacatcgggc agatgtccat cattggatga tgtttggtgg
     4261 gatgatcgcc atgttggcgt tcttcatcag ctactatctc tttcgccaat taggcgtgtt
     4321 ggcctttgaa gggaaagagg ggttcggggg atctgaagcg ctctaccata atgtgttcat
     4381 cccgatcctc attgttcata tcgtgcttgt ggtcgttggg ttagttatgg cggtctatat
     4441 gattgtcctg gggttccgag cacagttgat cgagcaaggg cgacggatct tgagcgatcg
     4501 cgttctgcaa acgacgtggg gaaagatcgg gatgattttc ggtatcgtct gtctgccggt
     4561 ggcgctgtat ttactgtatc tgatggctgt cgatcgtctt cggatgggac ggctcgtggt
     4621 ctgggccgga ttcctcgcca ttatcgcggt tgtgttcgcc gtagaaatga cgattcagcg
     4681 tctgtggccc gacggcgggc accgccaccg agcgcttgga cgcttcacga tggccatcta
     4741 ctgtattttg tttgtgaccg gaaccaccac atacacgatg ctgtatcttt tgtatccagg
     4801 aaagatcgga tgagcgacag agtcgatatc caattgcgga ggggtggaag cgaggaatgc
     4861 ccatgctgac ctgtggctgc ggtcgttgga tgcataccga ggggatcgaa gaacgtgtgt
     4921 atgacgatgg gacacctcag tggc
//
LOCUS       DATIPF010000037         4069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1242377, whole genome shotgun sequence.
ACCESSION   DATIPF010000037 DATIPF010000000
VERSION     DATIPF010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242377"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /gene="infC"
                     /locus_tag="VLL06_00930"
     CDS             <1..421
                     /gene="infC"
                     /locus_tag="VLL06_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HSS29560.1"
                     /translation="LTADAFKQAQENGYDLVEVAPTSVPPVCRIMDYGKYKFELSKKD
                     HQNRRHQKSTQVKEIKLRPRTDKHDLEIKIRQIKGFLADGNKTKVLLTYRGREMANQE
                     MGRTLMNTVIAELGEIGTIEYAPRMEGRSLIMIVAPK"
     gene            464..667
                     /gene="rpmI"
                     /locus_tag="VLL06_00935"
     CDS             464..667
                     /gene="rpmI"
                     /locus_tag="VLL06_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003851299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HSS29561.1"
                     /translation="MKKRKMKTHSGAKKRFSRTGSGKLVRRKAGRRHILTSKTQDRKR
                     RLSGTVLVDKTKTQALDRLLPYS"
     gene            711..1079
                     /gene="rplT"
                     /locus_tag="VLL06_00940"
     CDS             711..1079
                     /gene="rplT"
                     /locus_tag="VLL06_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HSS29562.1"
                     /translation="MPRAKGGPKTRHRRKKRLKLAKGQYGGKSRLFRTATESVDKGQA
                     YAYTGRKNRKRDFRVLWIARINAAVRAHGLTYGRFINALKKANILLDRKVLSDMAIKD
                     ATGFEQLVGLAKQQLAPAGA"
     gene            complement(1166..2884)
                     /gene="pheT"
                     /locus_tag="VLL06_00945"
     CDS             complement(1166..2884)
                     /gene="pheT"
                     /locus_tag="VLL06_00945"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HSS29563.1"
                     /translation="MPTITIFKRDLEGLLAEPGEVHQPITMEQLEESLMLVKGELKGQ
                     NPDTGELRIELQDSNRPDLWCCEGIARQIRIKRQGSLAKYDFLTGKSQSPKQLNVAPG
                     LEQIRPYVAACAATGYRVTQEGLAQLIQTQEKLAEMFGRKRRTVSIGLYRLAAIEFPV
                     TYDVVRPDEVTFTPLGMDTVMTLGEMLLVHPKGLEFGGILAGHDRLPYLRDATRQALS
                     FPPIINSREVGEVRPGDDALFVEVTGTDLSMVILTLNIFAANLADRGAVIEPIEVRYP
                     YKTALGKAVMTPHDLGKSRKLSVSTIEQALGQALGTHEVAKALTAYGYQVTTKGKEVR
                     VTLPLYRQDLMHAMDVVEDVAISRGYSDFLPVMPSQFTVGGLSRIEEISDHIRALMVG
                     MGFQEIISNILASPRDLRDAMRLEGTEWGKLVEVDNVMSQSFSALRQWMLPSLLRVEA
                     ASNRAFYPHRMFEAGEVARHDLSQAEGSRTVMVLGGMIAHADAHFSEAHSCLDTLFYY
                     VNQAYSLEPIQHPSFLAGRVGSIVSDGKHIGVIGELHPEVLERWQIGIPAVAFEIDLT
                     ELTERP"
     gene            complement(2892..>4069)
                     /locus_tag="VLL06_00950"
     CDS             complement(2892..>4069)
                     /locus_tag="VLL06_00950"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF003210.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HSS29564.1"
                     /translation="ALLIIQGGCIESTGRPSQTAEAMRGLLQQLHGAPRELRAFPEAQ
                     RQVIQDHAVKRGNAREPFRVDDRVARALKLSISGIEAAQQLSRQGTAEEVSQLTPELL
                     KDGSWRMKRFRKYTISLRAPRIAPGKRHPYREFLDTVKTKLVSMGFQEMRGSIVETEF
                     WNMDALFMPQFHPARDIHDVYFVKEPTHASSIPEPFLSQVAQAHQNGGETGSTGWNYP
                     FDAERARRLVLRSQGTAVSAHTLAAQPVVPGKYFSIARCFRYDQVDATHATDFFQVEG
                     IVLGEDINFRTLLGLLNLFAREVAQAKEVKFLPAYFPFTEPSVELHVRHPKLGWMELG
                     GAGLFRPEVTLPLGVTVPVIAWGLGLDRMAMIALGIHDIRELFTDNLDLIRSTRGAP"
BASE COUNT          970 a         1118 c         1143 g          838 t
ORIGIN      
        1 cctcacggcg gatgcattca aacaggcaca agaaaatggc tacgaccttg tcgaggtcgc
       61 accgacatca gtgccacccg tgtgccgtat tatggactac gggaaataca agttcgagct
      121 gagcaaaaag gaccaccaga accggcgcca tcaaaagtcg acgcaggtca aagaaattaa
      181 gttgcgtccg cggacagata agcatgactt agagatcaaa attcggcaga tcaaagggtt
      241 tctggccgat gggaacaaga ccaaagtcct cctgacgtac cgcggccgtg aaatggcgaa
      301 tcaggagatg ggccggactc tcatgaatac ggtcatcgca gagctgggag agataggcac
      361 gattgagtac gctccacgca tggaaggtcg aagtttgatc atgatcgtgg ctccgaagta
      421 agaccgtcgg tccgaatgct attaaccaaa ggaatcaccg atcatgaaaa agaggaaaat
      481 gaaaacgcat agcggtgcca aaaagcggtt ttcccgcacc ggctctggaa aattggtcag
      541 gcgaaaggcg ggaagacgcc atatcctcac cagcaagaca caggaccgga aacgtcgatt
      601 aagtggcacg gttctcgtgg ataagaccaa gactcaggca ctggatcgct tattgcccta
      661 tagctaaatc ggacaataca catcgcttca accaaaagga gtactgattc atgcctcgcg
      721 ctaaaggtgg ccccaaaaca agacatcggc ggaagaagcg gctgaaactg gccaaaggac
      781 agtatggagg gaagagccgg ctgttccgca ctgcaacaga atcagtcgat aagggtcagg
      841 catacgccta taccggacgg aaaaaccgga aacgggattt ccgcgtgtta tggattgcgc
      901 gtatcaacgc ggcagttcgg gcgcatggac tcacctatgg ccgatttatc aatgctctga
      961 agaaggccaa tatcctcttg gatcggaagg tcttatccga tatggcgatt aaggatgcca
     1021 cgggatttga gcagctggtc ggtcttgcca agcagcaact cgcccctgcc ggggcgtaag
     1081 cacagtcgcc taccaaaggt gcccttcgac atattggttt ggtgaggatg tcacgtcttg
     1141 aggagtgcgc cgacgggcgt agtcctcaag gccgttcggt aagttccgtc agatcgattt
     1201 cgaacgcgac agccgggata ccgatctgcc atcgttcgag aacttctgga tgcagttccc
     1261 caatcacacc gatgtgtttc ccgtcagaca cgatacttcc tactcgccca gcaagaaatg
     1321 aaggatgctg gatcggttcc aggctgtacg cttggttcac atagtaaaac aacgtgtcca
     1381 ggcaggagtg ggcctcggag aaatgtgcat cggcgtgggc gatcatcccg ccgagcacca
     1441 tcacagtgcg cgatccttca gcttgcgaga gatcgtgacg cgccacttca cccgcttcga
     1501 acattcgatg gggataaaat gcccggttcg atgcggcttc cacgcgaagc aacgacggaa
     1561 gcatccactg tcgaagagca gaaaagctct gggacatcac attgtctact tcgaccaatt
     1621 tcccccactc ggtcccttcc agccgcatcg cgtcgcgaag atctctgggc gaagcgagaa
     1681 tatttgaaat aatttcctgg aaacccatac cgaccatgag ggccctgata tggtcggaga
     1741 tctcctcaat gcgcgacaat ccaccgaccg tgaactgcga gggcatgaca ggtaaaaaat
     1801 cactgtaccc gcggctgatc gcgacatctt caacgacgtc catcgcgtgc attagatcct
     1861 gccgatagag cggaagcgtg acccgtactt ctttaccttt ggttgtcacc tgatagccgt
     1921 aggccgtgag agccttcgcg acttcgtggg tgccgagcgc ctgcccaagg gcctgttcga
     1981 tcgtactgac ggaaagcttc cgtgactttc cgagatcgtg tggagtcatg acggctttgc
     2041 cgagtgcggt cttataggga tagcgaactt caatcggctc aatcacagca ccacgatccg
     2101 caagattggc tgcaaaaatg ttgagggtca ggatgaccat cgagagatcg gtgccagtca
     2161 cttcgacgaa gagggcatcg tctcccgggc gtacttcccc gacttcgcga ctattgatga
     2221 tcggcggaaa ggagagggcc tgacgtgttg catcgcgcag gtatggcaga cgatcatgtc
     2281 ccgcgagaat cccgccgaac tccagaccct tgggatgaac gagcaacatc tcccctaggg
     2341 tcatgaccgt atccatgccc aatggcgtga atgtgacttc gtccggcctc accacatcgt
     2401 aggtgacagg aaactcaatg gctgcaagcc gatagagtcc gatcgatacg gtacggcgtt
     2461 tacggccaaa catctcggcg agcttttctt gtgtctgaat cagctgggcg aggccttcct
     2521 gggtcacgcg gtagcctgtc gcagcacagg ctgcgacata gggtctgatt tgctccagac
     2581 cgggggccac gttcaattgc ttgggcgatt gtgatttgcc cgtcagaaaa tcgtacttcg
     2641 cgagggagcc ctgccgcttg atccgaatct gtcgcgcgat cccttcgcag caccagagat
     2701 cgggacgatt actgtcttgc aattcaattc gcagctcacc ggtgtcgggg ttctgaccct
     2761 tcaactcgcc tttcacgagc atcaacgact cttcgagctg ctccatggtg atcggttggt
     2821 gcacctcgcc aggctccgcg agcaacccct cgagatctcg cttaaaaata gtaatcgtag
     2881 gcatgtgaaa cctagggagc ccctcgagtg gaacgaatca gatcaagatt gtcggtgaac
     2941 aactcacgaa tatcgtgtat gccaagggcg atcatcgcca tgcgatcgag tcccaaaccc
     3001 cacgcaatca ctggaacggt cacgcccagc ggaagggtga cctcaggtcg gaacaacccg
     3061 gctcctccga gctccatcca tccgagcttc ggatggcgca cgtggagctc gaccgacggt
     3121 tcggtgaacg gaaaataggc ggggagaaat ttgacttcct tggcttgtgc cacttcacgg
     3181 gcaaagagat tcagcaggcc gagcaacgtg cgaaagttaa tgtcttcgcc aagcacgatc
     3241 ccttcgactt ggaagaaatc ggtcgcgtgc gtcgcatcga cttgatcgta gcgaaagcaa
     3301 cgggcgatag aaaaatactt tcccggcaca actggttgtg cggccaatgt atgggctgag
     3361 acagccgtcc cctggctccg caagaccaac cggcgcgcgc gctccgcatc gaaaggatag
     3421 ttccaaccgg ttgaaccggt ttcaccgcca ttttggtgcg cttgcgcgac ttgggacaag
     3481 aacggttctg gaatcgacga ggcatgagtc ggttccttga caaagtagac gtcatggatg
     3541 tcgcgtgccg gatggaactg cggcatgaag agggcatcca tgttccagaa ttcggtctcc
     3601 acgatggagc cccgcatttc ctggaatccc atactcacga gcttggtctt caccgtgtcg
     3661 agaaactcgc ggtagggatg ccgtttgccc ggcgcaatcc gtggcgcgcg aagactgatg
     3721 gtgtacttac ggaagcgttt catcctccag cttccgtctt tcaatagttc gggtgtcagc
     3781 tgagagacct cctctgcggt cccctgccga gagagctgct gagcagcctc gataccgctg
     3841 atcgaaagct tgagcgctct ggctacccga tcatcaacgc ggaagggttc ccgtgcattc
     3901 ccgcgtttga ccgcgtggtc ttgtatgacc tgccgttgtg cttcgggaaa tgcccgcagt
     3961 tcacgaggcg ccccgtggag ctgttgcagc aatccccgca tcgcttccgc ggtctggctc
     4021 ggtcgaccgg tcgattcgat acagccgcct tgaatgatga ggagcgcgc
//
LOCUS       DATIPF010000038        13221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1154219, whole genome shotgun sequence.
ACCESSION   DATIPF010000038 DATIPF010000000
VERSION     DATIPF010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 13221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13221
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154219"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /locus_tag="VLL06_00955"
     CDS             complement(<1..1524)
                     /locus_tag="VLL06_00955"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HSS29565.1"
                     /translation="MSPLATALAGEGGRPCLTGLHGSSAGLALTTLLHPQVGTVGNRS
                     WLIVTGSDEAAERLFDDLHFFHGLIGLQTTPLALFPRWETLPYEGSAPHVGLIARRMN
                     TLHHIRTAPQTCLVTSIAALMQRLLPVMAFTNTILHFKRGGTIERETLIGGLFRLGYR
                     RVSVVEIPGEFSVRGGIVDIFSTAYADPLRVEFLGETVDSLRLFDPATQKSTAKVDRA
                     VVLPAREYLRAPTLPDALAPIPVDAEWRAPDLYTDMDTLFDYFDERPLLVLDQPAALE
                     RACADLWSKIDDGYLRHADRETVTPYPSPERLFLSWPELMARTNGWSTLALEPLTARD
                     ESWSPVQAFPAQTPASAGLGARGMPFSQTLAIMDRLREACRVVLVARSRGQVDRLLAL
                     LREHDVPAMEWTTGAWTASSKTKAPFFALHGDLSAGFLAPDLRLAVLTEEELFAKGAR
                     HKPQAKSKAATFLSSLEDLNVGDYVVHVQHGIAKYQGLKRLSVQDFDSDYLILEFAGS
                     "
     gene            complement(1567..2049)
                     /gene="rfaE2"
                     /locus_tag="VLL06_00960"
     CDS             complement(1567..2049)
                     /gene="rfaE2"
                     /locus_tag="VLL06_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HSS29566.1"
                     /translation="MSVKVMTRDQLAPLLHTAQTQGKRVVFTNGCFDLMHIGHTRYLQ
                     AAKDLGDLLVVGVNSDASVRSLNKAPDRPIVSDTQRAEVIAALGCVDYVILFNEPDPR
                     GLIAALQPDVLVKGGDWPVERIVGREIVEARGGLVKTIPLVPDISTTSLIQRIRAQSI
                     "
     gene            complement(2096..3250)
                     /locus_tag="VLL06_00965"
     CDS             complement(2096..3250)
                     /locus_tag="VLL06_00965"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HSS29567.1"
                     /translation="MRWPRQILFGDVDAMFASAAVLADPTLADQPVAVGGPAPRGIIA
                     AASYAVRSFGVHSAMPTAEALRLCPHLILVPPDRPLYRRLHEQMQAVTDRLFPLTEWS
                     SIDEFYADTTELQTRHPDPRPLGQLVKDTLFDATGLRCTVALATGKTVAKIAADSHKP
                     DGLVTVPPGTEAEFLLQRPLRAMPGIGPKTYARLEPLGLKTIGELLDPRWDHSLRHIF
                     GTRLHWLRELARGIDHEPVVADRESKSLSHETTFDHDTQDRPFLEHTLRGFLRDLASD
                     LRQDGLAAGSCTVKLKNTRFVITTKQHRFPHPLNYDPDMWPTIQRTLQELVRPDTEYR
                     LAGLALSSLTPAPPGLFDQRRTKAIETMDALIAKHGSSVIGLGGVSGDDQ"
     gene            complement(3274..3927)
                     /locus_tag="VLL06_00970"
     CDS             complement(3274..3927)
                     /locus_tag="VLL06_00970"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HSS29568.1"
                     /translation="MSPRQSPPVTSQHLTQIWSELNGRYFAGLLPPIDLVWSRRLTSS
                     VGMFVSHRGPRPRLDQNGLRPQTKREIRLSLPLLKQAAQRSEYGEQEIVSTLAHEMIH
                     QWQYDILKRRPNHGADFLRKMTEMNRDGALAITVYHSLQKEVLALTRFAWRCRQCGRV
                     YRRQRRSIQPRRHHCGICRGSLQELGSVSRIHHEPAATPSPPLLPFVQLSFTFLAGC"
     gene            complement(3938..4930)
                     /locus_tag="VLL06_00975"
     CDS             complement(3938..4930)
                     /locus_tag="VLL06_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29569.1"
                     /translation="MAKPPDRQRPPVTTPSLVPPINVTDLTTYPLKKRYSKVRVSDFA
                     KPWGLGGSFNQFYRSLPDILGVKTLRAVAKAVAKAHRKGRPVIVGIGAHVVKVGLSPI
                     LVDLMRQGIVSAVAMNGAGIIHDFELALMGHTSEEVDAEIDEGRFGMAEETGRMLNEA
                     IVRGAHDREGLGESVGHYINRRQGQFPHRETSILAIGARLGIPVTVHVAIGTDIIHMH
                     PAADGAAIGATSLLDFRRLAAVVSGMEGGVYLNLGSAVVLPEVFLKTVSLARNLGHDL
                     TNFTTVDMDFLAHYRPLTNVVRRPTQKGGKGYSLIGHHEIMVPLLAAAVYEELG"
     gene            complement(4914..5492)
                     /locus_tag="VLL06_00980"
     CDS             complement(4914..5492)
                     /locus_tag="VLL06_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HSS29570.1"
                     /translation="MKEHVLSTFAESATVKQQFAREHADRIVQVAKLIARAFRDGHKV
                     LLFGNGGSATDAAHLAAEFVGRYKRERAPLPAIALATDIAAITSIANDYGFDELFARQ
                     IRAHGQKGDIAIAISTSGNSPNVLKGVDAAQACGLTTIGWTGGTGGKLAGLVEYPFVV
                     PSTVTARVQESHITLGHVLCELIEEQLLGKAS"
     gene            complement(5547..6485)
                     /locus_tag="VLL06_00985"
     CDS             complement(5547..6485)
                     /locus_tag="VLL06_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HSS29571.1"
                     /translation="MSGGLFVVLLLAVLVLVVIAKTAVVVPQQSAYVVERLGKYATTL
                     DAGFHILLPFIDRIRYKHSLKETAIDIPEQVCITRDNVQVSVDGILYLKVLNPQRASY
                     GISDYNFALIQLAQTTLRSEIGKIELDRTFEERTNINIQVVNELDKASDPWGVKVLRY
                     EIKNITPPKGVLDAMEKQMRAEREKRAVILTSEGERDAAINQAEGEKQQVIKASEAKK
                     QQQINEAEGAAAAILAIATATAEGLRKVAETIRVPGGQEAVQLRVAEQYITKFGELAK
                     TTNTLILPASVSDVGSMIAVAMNAMRQTSITPLQKS"
     gene            complement(6529..6963)
                     /locus_tag="VLL06_00990"
     CDS             complement(6529..6963)
                     /locus_tag="VLL06_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HSS29572.1"
                     /translation="MIWWYWMLLGIVLLGIEMVTPGGFYILFFGLAALILGGLTSLGF
                     AQAEWLQWLLFSGLSILSLLVFRGPLLAWIKTKGKDMPTVDSMVGESAILLEDLAPGG
                     TGKAELHGTTWTVHNAGQAPVKKNQRCKVERVEGLALWIAAE"
     gene            7251..8507
                     /locus_tag="VLL06_00995"
     CDS             7251..8507
                     /locus_tag="VLL06_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02710 family CRISPR-associated CARF protein"
                     /protein_id="HSS29573.1"
                     /translation="MAQDQPVKALVLALTDHAAAAVYSINRLQPDFLCFVLPEGAKSL
                     VETDVQPKIEHMPRRWDWVVMAETCEFVACYQTLARTLPDMLQTWDVQPGELVVDVTG
                     ATPAMAGALMLATRSLSSRIVSLVPAREGQEGDRIDIAGRPFVWTQGDPWDEAASVSR
                     REGCELFNRRLFGAAAKLFREVEARVSGGQKPLYRAFADLADGYHLWERFQYRQAWEK
                     LKTAVKAFEMASLWGGPPGLTSLLPAIKANAGFLEKLVLDPAPVKEMQALDLLAHAGR
                     RLHGTQDPETAMISLVRGLEAFAQRQLFKLYNIKTWDIQPEQLPQALRETCRSCWLED
                     LDGKYKLPLQAQFRALAGLGDQMGQAFLREWPTMKPLLDAANHAVLGHGFEPVKVERV
                     QQLYDVVVKLSGASETSVPKFPTLNL"
     gene            8593..8994
                     /locus_tag="VLL06_01000"
     CDS             8593..8994
                     /locus_tag="VLL06_01000"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HSS29574.1"
                     /translation="MDIQIYYEDTDCGGMVYYANYLKYFERARTHYLEERGLSVAGLL
                     KEGTVFVVVHAEVDYRSPARYGDRLIIDTVVSDMTAASFTFTHVIRERETQRVIVEGS
                     ARLAATNGSGKVKRLDKATVSVLQFSPTRSH"
     gene            complement(9456..9899)
                     /locus_tag="VLL06_01005"
     CDS             complement(9456..9899)
                     /locus_tag="VLL06_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29575.1"
                     /translation="MPDLPNELIRVNKILHDYTAIHDAIFKFSWRKALPIPGLFKPTD
                     FGAHVKDLSRLASKLTTLSSALKADSEALEGSHQYAAALLEAVQALREICGRFYEKSQ
                     GNPGKYPMAEYNADLKAYEGLVNMCQELGVALNQRIRNDHVQPED"
     gene            complement(9963..10463)
                     /locus_tag="VLL06_01010"
     CDS             complement(9963..10463)
                     /locus_tag="VLL06_01010"
                     /inference="COORDINATES: protein motif:HMM:NF025675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HSS29576.1"
                     /translation="MSHEATLIYSESLLREAVFGFWRRSIGMGFILMLVALTVVLGVL
                     VALGAPVWIIVTLAVLLVLAAAVAVALYIGYYRNSLRKFRTMSTPRATFRADESSFTM
                     TSDAGTTTLQWSAVKELWQFPSVWLLLYSKTQFSTLPLACLPPDIQAFVQERVREAGG
                     KVAANP"
     gene            complement(10567..10773)
                     /locus_tag="VLL06_01015"
     CDS             complement(10567..10773)
                     /locus_tag="VLL06_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29577.1"
                     /translation="MTSQANTPLKPAGKIPWYVYFVGAIIYTILASWLFKESDSPVLA
                     WLCLISSPVGFFYAWRAWKQDQHK"
     gene            complement(11479..11847)
                     /locus_tag="VLL06_01020"
     CDS             complement(11479..11847)
                     /locus_tag="VLL06_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSS29578.1"
                     /translation="MTPIRSNAVFHVSDIDRSIDFYANCLGFKIDFRLGDPVSYAGLS
                     LGNVRLHISSSYPYKNNAGHGNLYITADEVDSLYHELVEAGVEFYCSIGNREYGLRDF
                     AVKDPDENQIAIGTVLHESA"
     gene            complement(11984..>13221)
                     /locus_tag="VLL06_01025"
     CDS             complement(11984..>13221)
                     /locus_tag="VLL06_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29579.1"
                     /translation="ANFKNATPGKRLVSFIAKADGFESRRSIQQIFVSQTRFDSSTNT
                     YTCAVEEGTLTVSNISAIVQRPEWGKDREGKCECPPVSGPWVPTGMTLAWAPTPAFAG
                     VHGDLPFSDPWWKILAIIVAILAAIVAIVAAALGAGTASFGASGTFEETDPSVTCCTP
                     DVGAGPKKEFTVAGVASAIATVAAAVALSDAADPFWRGQEKTPPTAGELTIGEKVLAK
                     WSLPDPPNAGKPYTTDVKWTYTRFTTGKTYEYSVSETQTNIHVSDAIEVETPATVHPF
                     KPLWVRSKFHRTGANLFKGSELYAFALFRSPSGLYFVVPMTDDGLGFDPGANDGTYAG
                     SLDLEHAYRLLLKYYQDVYGIWRVYVFAQDVNQTMPGTPPEIAAQHIGGFFVASAISI
                     AFDPSLPCPLNAQGTITVV"
BASE COUNT         2802 a         3671 c         3924 g         2824 t
ORIGIN      
        1 gctgccggcg aattccagga tcaaatagtc gctgtcgaag tcctgcaccg agaggcgctt
       61 gagtccttga tatttggcga tgccatgttg gacatggacg acatagtcac ccacgttgag
      121 atcctccaac gaggagagga aggtcgcggc tttactcttc gcctgcggtt tgtggcgtgc
      181 gcctttggca aacagttctt cttccgtcag aaccgccagt cgcaagtccg gcgcgagaaa
      241 gcctgcagac aaatcgccgt gcaaggcgaa aaacggcgct ttggttttgc tggacgcggt
      301 ccaggcccct gtcgtccatt ccatcgccgg cacatcatgt tcacgtaaga gcgccaacaa
      361 gcggtccact tggccgcgac tccgcgcgac gagcacgacg cggcatgcct cccgcaagcg
      421 atccatgatg gccagcgtct ggctgaaggg cattccacga gctccgaggc cggcactggc
      481 cggggtctga gcgggaaagg cctggaccgg actccaggat tcatcgcgcg cggtcagcgg
      541 ctcgagtgcg agagtggacc agccgttcgt gcgagccatc agttccggcc aggagagaaa
      601 caaccgctcc ggcgacgggt agggcgtgac cgtctcccga tcggcgtggc ggagatagcc
      661 gtcgtcgatc ttgctccaga ggtccgcgca ggctctttcc aacgcagccg gctgatccag
      721 gaccaagaga ggccgttcgt cgaagtagtc gaagagggtg tccatgtcag tgtacagatc
      781 cggcgcgcgc cactcggcat ccaccggaat aggagccagc gcatcaggca aagtcggtgc
      841 acggagatat tcacgcgcgg gaaggacgac cgcgcgatcc acctttgcag tggatttctg
      901 cgtggcagga tcgaacaggc gtagcgaatc gaccgtttca ccaagaaatt cgacacgaag
      961 cgggtcggca taggccgtcg agaatatatc cacaatgccg cctcgaacac tgaattcgcc
     1021 cggtatctcc acgaccgaga cgcggcggta cccgagacgg aagagtccgc caatgagggt
     1081 ctctcgctcg atcgtgccgc cccgcttgaa atgcaagatc gtgttcgtga aagccatgac
     1141 gggcaacagg cgttgcatca gggccgcgat ggaggtgacc agacaggtct gtggtgcggt
     1201 tcggatgtga tgcagggtat tcatccgacg tgcgatcaga ccgacatggg gcgcgctgcc
     1261 ttcgtatggc aaggtttccc atctgggaaa cagggcgagg ggagtagtct gaagaccgat
     1321 gaggccatgg aaaaaatgga ggtcatcgaa gaggcgttca gccgcctcgt cggacccggt
     1381 gacgatcagc cacgaacgat ttcccacggt gcctacctga ggatgcagga gtgtggtgag
     1441 ggcaagcccg gcggaagagc cgtgtagacc ggtgagacaa gggcgacccc cctcaccggc
     1501 gagtgccgtg gcgagtggtg acaaccactg agtcgttgat agattggagg tgatgttcga
     1561 cacggtctag atggattgcg cgcggatccg ttggattagg gaggtcgtcg atatgtccgg
     1621 caccagtgga atggtcttca ccagtccacc gcgtgcttcg acaatctcgc ggccgacgat
     1681 ccgttccacc ggccaatctc ctcctttgac aaggacatcc ggttgcaatg cggcaattag
     1741 gccacgaggg tccggttcgt tgaacagaat cacatagtcg acacagccaa gcgcagcgat
     1801 cacttctgcc cgctgagtgt ccgagacaat cggccgatcc ggagctttgt tcaaacttct
     1861 aacagatgcg tcgctattga caccgacgac cagcaggtca ccgaggtctt tggctgcctg
     1921 tagataacgg gtgtgtccaa tgtgcatgag gtcaaaacag ccgttcgtga acacgacacg
     1981 tttcccttgg gtctgtgctg tgtgtagaag tggagcgagc tggtctctgg tcatgacttt
     2041 cacagacata tctgcatcat accgactgag ccctcggatg gaaagagaca gcatactact
     2101 ggtcgtcgcc gctcacgccg cccaacccaa tcaccgacga cccgtgcttg gcgatcagcg
     2161 cgtccatcgt ctcgatggct ttcgtccgcc gttggtcgaa cagcccgggc ggcgcgggtg
     2221 ttaaggagga gagtgcgagc ccggccaaac ggtactcggt gtcgggtcga acgagttcct
     2281 gtagcgtgcg ttgaatagtc ggccacatat cgggatcgta gttcaatgga tgcggaaagc
     2341 gatgctgttt ggtggtgatc acgaaacggg tgttcttcaa cttcaccgtg caggatccgg
     2401 cagccaatcc atcctgccgg agatcacttg ccaggtcgcg gaggaatccg cgaagggtgt
     2461 gctcaagaaa tggacgatcc tgcgtatcgt ggtcgaacgt cgtctcgtgg ctgaggcttt
     2521 tagactcccg atcagccacc actggttcgt gatcgatccc ccgcgccagc tctcgaagcc
     2581 agtgcagtcg cgtgccgaag atatgacgca gagagtgatc ccagcgtggg tccaacagct
     2641 caccgatggt cttgagtccg agcggttcca ggcgcgcgta ggttttggga ccgatccccg
     2701 gcatggcgcg cagaggccgc tgcaataaga actctgcttc cgtaccaggc gggactgtca
     2761 ccagtccatc cggcttatga ctatccgctg cgattttggc gacggtcttg ccggtggcga
     2821 gcgcgactgt gcaacggagt ccggtggcat cgaagagcgt atccttcacg agttgcccaa
     2881 gtgggcgagg atcgggatgg cgggtctgga gctccgtcgt gtcggcataa aactcgtcga
     2941 tgctgctcca ttcggtcaag ggaaacagcc gatcggtcac ggcctgcatc tgttcatgca
     3001 gccgacgata gagtggtctg tctggaggaa cgagaatgag atgggggcac aagcgtagag
     3061 cttcggcggt cggcatggca gagtgaacgc cgaatgaacg aacggcgtaa ctcgcggctg
     3121 cgataattcc tcgcggggcc ggtccaccga cggccaccgg ttggtctgca agggtcgggt
     3181 ctgccaggac ggctgctgag gcgaacatgg catccacatc cccgaacaga atctggcgag
     3241 gccagcgcat ggatcatccc ccaagacagg aagtcagcat cctgctaaga aggtaaagga
     3301 caattggacg aacggcaaga ggggaggaga gggtgttgca gcaggctcgt ggtgtatccg
     3361 actcaccgag cccaattctt gtaacgatcc ccgacagatg ccgcaatggt gccgccgggg
     3421 ctgaatcgac cgccgctggc gtcggtacac tcggccgcat tgccgacaac gccaggcgaa
     3481 tcgcgtgagg gcgaggactt ccttctgtaa ggaatggtag acggtgatcg cgagggcacc
     3541 gtcccgattc atctcggtca tcttgcgaag aaaatccgcg ccgtggttcg gtcgccgttt
     3601 caagatgtcg tactgccatt gatgaatcat ttcgtgtgcc agcgtgctca cgatctcctg
     3661 ctcgccgtac tcgctccgtt gggcggcctg cttgagcaga ggaagggaga ggcgaatctc
     3721 tcgcttggtc tgtgggcgaa ggccattctg atccagtcgg ggccgcggcc ctcgatgact
     3781 gacgaacatg ccgaccgacg aggtcaatcg gcggctccag acgaggtcga tcgggggcag
     3841 cagacctgcg aaatagcggc cattcagctc gctccatatc tgggtcagat gctgtgaggt
     3901 cactggaggg gattggcgcg gagacatgtg gaggggatca ccccaattct tcgtagaccg
     3961 ctgcggcaag caacggcacc atgatttcgt ggtggccgat cagagagtaa ccttttccgc
     4021 ctttctgtgt cggtcgacgt accacgttgg tgagcggacg gtagtgagcc aagaagtcca
     4081 tgtccacggt ggtaaagttt gtgagatcat gaccaagatt gcgtgccagt gacaccgttt
     4141 tgaggaagac ttccggcaac acgacggcgg agccgagatt gagatagacg cctccttcca
     4201 tccctgacac gacagctgca agccgtcgaa agtccagcag cgaggtcgct ccaatcgcgg
     4261 ctccgtctgc ggcaggatgc atatggatga tgtcggtccc gatggcgacg tggacagtca
     4321 ccggaatacc gagccgtgct ccgatcgcca gaatgctggt ctcgcgatgg ggaaactgtc
     4381 cctggcgccg gttgatgtag tgaccgacgg attcgccgag cccctcacgg tcgtgggccc
     4441 cacggacgat cgcctcgttc agcatgcggc cggtttcctc cgccatcccg aagcgtccct
     4501 cgtcaatctc cgcatcgact tcctcagacg tgtgtcccat gagcgccaat tcaaaatcgt
     4561 gaatgatccc ggcgccgttc atggccacgg ccgacacgat tccttgccgc atgaggtcca
     4621 cgagaatggg actcaatccg actttgacga cgtgtgcgcc gattcccaca atgaccggtc
     4681 ggcctttacg atgggccttc gcgaccgctt tggcgactgc gcgcaatgtc ttcacgccaa
     4741 ggatatccgg cagcgagcga tagaattggt tgaagctgcc tccgagcccc cagggcttcg
     4801 caaagtcgga gacgcgaacc ttgctgtacc gtttcttgag cggatacgtc gtgagatccg
     4861 tcacgttgat tggcgggacc aggctcggag tcgtgacagg aggccgctgc cggtcaggag
     4921 gctttgccaa ggagctgttc ctcgatgagt tcacacaaga cgtggcctag cgtgatatgg
     4981 ctttcttgaa cgcgggccgt gactgttgag ggcacgacga acggatactc caccaggccg
     5041 gccagttttc ctccggtccc tccggtccag ccaatggtcg tcagaccaca ggcttgcgcg
     5101 gcgtctacgc cttttaagac gttgggtgaa ttgccgctcg tgctgatggc aatggcaata
     5161 tcaccttttt gtccatgggc gcgaatctgg cgggcaaaga gttcgtcgaa accatagtcg
     5221 ttggcgatag acgtgatggc ggcaatgtct gtggccaggg ctatggccgg aagcggggct
     5281 cgctcgcgtt tataccgccc gacgaactcg gcggcgaggt gggccgcatc cgtggcactg
     5341 ccgccattgc cgaagagcag cactttatgt ccgtctcgaa aggccctggc gatcagtttt
     5401 gcgacctgta cgatgcgatc ggcatgctct cgcgcaaact gttgcttgac agtcgcgctt
     5461 tcggcaaacg tactgagcac atgttctttc atggaaagcg attctaggca acctgctgtc
     5521 acctgtcaaa cgtgattcgc agactcctag gacttttgca gaggagtgat cgaagtctga
     5581 cgcatggcgt tcatcgcgac ggcgatcatg gagccgacat cggagaccga tgccggcagg
     5641 atgagagtat tggtcgtttt ggccaactcc ccaaacttcg tgatgtattg ctccgccacg
     5701 cgcagttgaa cggcttcctg acctcccggc actcgaatgg tctccgcgac cttgcgcagc
     5761 ccctctgcgg tggccgtggc aatagccagg attgctgcgg ctgctccctc cgcttcgttg
     5821 atctgttgct gcttctttgc ctcagaggct ttgatgactt gctgtttctc accctcagcc
     5881 tggttgattg ctgcgtcgcg ctcgccctcg gacgtgagaa tcaccgcgcg cttttcccgt
     5941 tccgcacgca tctgcttttc catcgcgtcc aggacaccct tcggcggggt gatgttcttg
     6001 atctcatagc gtaagacctt gaccccccaa ggatccgagg ccttgtcgag ttcgttcacc
     6061 acctggatat tgatattggt ccgttcctcg aacgtccggt ccagttcgat cttgccgatc
     6121 tcgctccgca gtgtggtctg cgcaagctgg atcagagcaa aattatagtc gctgatacca
     6181 tatgaggcac gctgagggtt caagaccttg agatacaaga tgccgtccac cgatacctgc
     6241 acgttatcgc gcgtgataca gacctgctcg ggaatatcga tggccgtttc tttgagcgaa
     6301 tgtttgtagc gaatgcggtc gatgaagggg aggagaatat gaaaccctgc atccaacgtg
     6361 gtcgcatatt tgccaagccg ctcgaccacg tacgcgcttt gctgtggcac gaccacggcg
     6421 gtcttggcaa tcaccaccag caccagcacc gccagtaaca gcaccacaaa caatccgccg
     6481 ctcatcgtta cctcctccat cgtcgttcgc cctccgggcg ctcctcactc attcggctgc
     6541 aatccataag gcgagcccct caacccgttc gaccttgcac cgttgattct ttttcacggg
     6601 ggcctgcccg gcgttatgaa cggtccatgt ggtgccatgc agttccgcct tgccggtacc
     6661 gcctggcgcc aggtcttcaa gcaggatcgc gctttcgccc accatcgagt ccacagtcgg
     6721 catgtccttg cccttggttt tgatccaggc caacaatggc ccgcgaaaga ccaggagtga
     6781 gaggatcgag agccctgaaa acaaaagcca ctggagccat tccgcttggg cgaaccctag
     6841 gctggtcaga cccccgagga tcagagccgc cagtccgaag aagagaatat agaatcctcc
     6901 cggcgtcacc atttcgatgc cgagcagcac aatgccgagg agcatccaat accaccaaat
     6961 catcgggggg cctcattcag cattcgaacg aattgcgagg gaattgggga tcgtagtaaa
     7021 agtctaagcg cctttactga gaccgtcaag cagaacgcac gttactcaat tgtgaattga
     7081 tcgcatgcga ggccgatgac tcatcacggc aaggccgccg cgcgcgaaag cccggtgagg
     7141 tacacacgcc actttgcgtg ggttgtgtgc ttctcgaatg gaccttgatc agggaaggga
     7201 gaccagagcc ggaaggcttt tacgccgtcc tatgctatag tccgcgcatc atggcgcagg
     7261 accaaccagt caaagcctta gtgcttgcgc tcactgacca tgccgccgca gcagtctatt
     7321 cgattaatcg tctgcaaccg gatttcctct gcttcgtgtt gccggaaggg gcgaaatcgc
     7381 ttgtagaaac ggatgtccaa ccgaagatcg aacatatgcc aagacggtgg gattgggtgg
     7441 tgatggcaga gacctgcgaa tttgtcgcct gttaccagac gcttgcccga acactcccgg
     7501 acatgttgca gacatgggat gtacagccag gtgagcttgt cgtcgatgtc acgggagcca
     7561 cgccggccat ggcgggggcg ctcatgctgg ccacacgatc tctcagttca cggatcgtgt
     7621 cacttgtccc ggcgcgagag ggacaggagg gggacaggat tgatattgcc ggacggccgt
     7681 tcgtctggac gcagggggat ccgtgggatg aagcggcatc ggtctctcgt cgcgagggct
     7741 gtgagctctt taaccggaga ttgtttggcg cagcggcaaa gctgttccgc gaggtcgagg
     7801 cacgggtgag cggcgggcag aaacccctgt accgtgcctt tgcggatctg gccgatggct
     7861 accatctgtg ggaacgattt cagtatcgtc aggcctggga gaagctgaag actgccgtga
     7921 aggccttcga gatggcatcc ttatggggcg gaccaccagg tctcacatcg ctgttaccag
     7981 ccattaaagc gaatgcggga ttcttggaaa agctcgtcct cgatcctgct cccgtgaaag
     8041 aaatgcaggc actcgatctg ctggctcatg ccggccggcg attgcatggt acgcaggatc
     8101 cggaaactgc gatgatctcg ctcgttcgtg ggctcgaagc ctttgcccag cgacaattgt
     8161 ttaagctgta caatatcaaa acatgggaca tccagccgga gcaattaccg caggcactac
     8221 gggaaacctg ccgatcctgt tggttagaag atctcgatgg caagtacaag ctgcccctgc
     8281 aggcgcagtt tcgggcactg gccggactgg gcgaccagat gggccaagcc tttttgcgcg
     8341 aatggccaac gatgaaaccg ttgctcgatg ccgccaacca tgctgtcctt ggtcacgggt
     8401 tcgagccggt caaagtcgag cgggtacaac agctctatga tgtggtcgtc aagttatcgg
     8461 gcgccagtga gacatcggtg ccgaagtttc cgaccctgaa cctctaatgc gacaagggag
     8521 attcgcatgc aaggagaaac aggcaatggg tgaaggatcg tgtctgtcat gcgtgtttcg
     8581 cgagtagagt agatggacat tcaaatttat tacgaagaca ctgactgcgg cgggatggtg
     8641 tattacgcga actatctcaa atatttcgag cgggcgcgga cacactatct tgaagagcga
     8701 gggttatcgg tggccgggct cttgaaagaa ggcacggtct tcgtcgtggt gcatgcagaa
     8761 gtggactacc gatctccagc ccgttatggc gaccggctaa tcatcgacac ggtcgtctcc
     8821 gacatgacgg cagcctcatt cacatttacg cacgtgatcc gggaacggga gacccagcgt
     8881 gtgatcgtcg aaggatcggc aaggttggcg gcaacaaacg gcagcggcaa ggtgaagcgc
     8941 ctcgacaagg ccacggtgtc cgtgctacag ttttctccta caaggagtca ctaaatgtcg
     9001 aagtgggctg ggccagagtt cggattgatg cagataggga gctggctcat tgtcatcggc
     9061 gtttgggcca ctatcatctt ctttgcctgg aaattcttca tggaaaaccc gatggcaaaa
     9121 actgggaagg acaggaggaa gctagaatca gaaaaccggt cgtctctccc acctgactct
     9181 caccagcact agcaggatac aaaacacccg ccagtaccag atgaccgtta tggggtctgc
     9241 tggcgctctg gtttctctcg tctattcgct tagtctggtt taaccacaca aggtcaacct
     9301 gcctctccca tctccctcga ctatcctggt agaaggtata gtctctatag cagcgatcag
     9361 aaagatcggt ctacctccgt gtcagtggta gctgtcattt catatggcgg aattcctcgc
     9421 ctgtcagagc agtagggtct caacggcatt gtcgctcagt cctccggttg gacatggtca
     9481 ttgcgtatgc gctggttgag tgcaacgcca agctcttggc acatattcac gaggccttca
     9541 tacgccttga ggtcggcgtt gtactcggcc atcggatact taccaggatt gccttggctc
     9601 ttttcataaa accgcccgca gatttcccgg agtgcttgga cggcttcgag caatgctgcc
     9661 gcgtactggt gggaaccctc cagggcctct gaatctgcct tcaatgcaga tgaaagagta
     9721 gtcaattttg acgccaaccg actgagatcc ttcacgtgag ctccgaagtc ggtgggtttg
     9781 aacaggcctg ggatcggaag tgccttgcgc cacgaaaatt taaagatcgc atcgtgaatg
     9841 gcggtgtagt cgtgaaggat tttattgacc cggatcaatt catttggaag atccggcatg
     9901 tgtcatcctc gagaccgtgc cccacgtacc aattgagccg atggcgaagc tcatcgtatg
     9961 aatcatgggt tagccgcaac cttgcctccg gcttcccgga cacgctcctg cacgaaggcc
    10021 tgtatgtccg gtggcaagca tgcaagcggc aacgtactga actgtgtttt ggaatatagc
    10081 agaagccaca cgctagggaa ctgccacagc tccttgacgg cggaccactg aagcgtcgtc
    10141 gtcccggcgt ccgaagtcat ggtgaacgaa gactcgtctg cacggaacgt cgctcgaggc
    10201 gtgctcatcg tgcggaactt gcgcaacgag ttgcgataat acccgatgta gagcgccaca
    10261 gcgaccgctg cggcgaggac gagaagcacc gccagggtca caatgatcca aacgggcgca
    10321 ccgagcgcga cgagtacacc gaggaccact gtgagagcca cgagcatcag gatgaagccc
    10381 atgcctattg agcggcgcca aaacccgaac accgcctcac gcagcagcga ctcgctataa
    10441 atgagtgtgg cctcgtgcga catggcggtt aacgactcag gctcgagagc tcagcccacg
    10501 aggaatgtcg gttgtaacca tggtgggacg gcctggttct ctgcggcaca gcgtgaggca
    10561 tcgtttctat ttgtgttgat cttgtttcca cgctctccag gcatagaaaa accccacggg
    10621 tgaactgatg aggcaaagcc atgccagcac aggggaatcc gactccttga ataaccaact
    10681 ggcgagaatg gtatagatga tcgctccgac aaaatagacg taccagggaa tcttgccggc
    10741 tggtttcaac ggtgtattgg cttgcgatgt catccgaggt ctcgatgggt ccggtgaagt
    10801 gtaactatgt atgatctgaa tccggataac aagccactcg ccttgggctg ctggacaaga
    10861 gtttttcact atcctgccag gatcggcgat ctaaatccaa gtgatatctt ccaggttttc
    10921 tcgcatcata tctatccagg gcgtgatgcg attccatagg agctacactc ctttagctag
    10981 attccacagg acaaccatgg gccccagacg ttgaaggtcc gagggcaagc cagctaggag
    11041 aaggttcgtc tagattatgg gctgagccaa ggacgatgct tcacagccat cttcgtacgc
    11101 ctttcttgta tgtgtcgtat tcattgccga atttggcaga caacacacgc tcctcaggcg
    11161 agatttgaaa gcggttcatg tacgcgacaa aggccggcaa aagcagggcg ggaatggtta
    11221 tggatgggct agatatgttg ccaaaccggt gtgggcgatc aggatgccaa cgtacatggg
    11281 gttctggctt atacgatcga cacctgatgt gacgacagat gatcaggtag tggttacttc
    11341 aaggtatctg agctgaatag aaagtctctg ttattctttc ctctcgatgt ctcacgttgg
    11401 aagtaaccgg cctccggagt gcgccgtagg gaggggatcc cacagcgcag ctcctcggca
    11461 gtccggttgg cggaagtgtt aagcgctttc atgaagcacg gtacctatgg cgatttggtt
    11521 ttcgtcagga tctttgacag cgaaatctcg cagcccgtat tcgcgatttc ctatggaaca
    11581 gtagaattcc actcctgcct ccaccaactc gtgatagagg ctgtcgactt catcggctgt
    11641 gatatataaa ttgccatggc cggcattatt tttgtaggga taggatgagc tgatatgcag
    11701 acgtacgttc cctaggctca gaccggcata ggaaacagga tcgcccaatc tgaaatcaat
    11761 tttaaacccc aggcagttgg cgtaaaaatc aatagatcta tctatatcac tcacatgaaa
    11821 cactgcattg ctacgaattg gtgtcatatt gttcttcctc tgtaccgtac gtcgggcatc
    11881 agccgactcc gtaggcggtc ggctgcctgc ccttcgttac gcgcgttctt ggaatttctg
    11941 catccttgac cattttcctt actgcatcga ccagaccttt ccttcagacc actgttattg
    12001 ttccctgagc attcagcgga caaggcagcg acgggtcgaa ggcgatacta atcgcgctgg
    12061 caacaaagaa ccctccgatg tgttgggcgg cgatctccgg aggtgtgccc ggcatggttt
    12121 ggttcacgtc ctgggcgaag acgtatacac gccaaatccc atatacatcc tggtagtatt
    12181 tgagcagcag gcggtaggca tgttccaggt cgagcgagcc ggcgtacgtt ccgtcgttcg
    12241 cacccgggtc gaacccgagt ccgtcatcag tcatcgggac cacgaagtac aggccactcg
    12301 gcgagcggaa gagagcaaac gcatagagtt cagatccctt gaacaggttg gcaccggttc
    12361 tatggaattt cgagcgtacc caaagcggct tgaacgggtg gactgtcgcg ggcgtctcca
    12421 cttctatggc atccgagacg tggatgttgg tctgggtctc gctcacagaa tattcgtagg
    12481 tcttgcccgt tgtgaatctc gtgtaggtcc acttcacatc ggtggtgtag ggcttgcccg
    12541 cgtttggagg gtcgggcagc gaccacttgg ccagcacctt ctcgccgatg gtcaattcgc
    12601 ccgcagtcgg cggcgtcttt tcctggccac gccagaaggg atcggctgcg tccgatagag
    12661 ccaccgccgc agcgacggtg gcgatcgccg aagcgactcc ggcaacggtg aactcctttt
    12721 ttggtccagc gccgacatcg ggtgtgcagc acgtgaccga cggatcggtc tcctcgaacg
    12781 taccgctcgc accgaacgaa gcggttcccg ctcccaacgc tgccgccaca attgccacga
    12841 tggccgccag aattgcgacg atgatcgcga ggattttcca ccagggatcg gaaaacggga
    12901 ggtctccgtg cacgcctgca aatgccggag tgggtgccca ggcgagcgtc atcccggtcg
    12961 gcacccacgg cccgctcacc ggtggacatt cgcactttcc ttcgcggtct ttcccccatt
    13021 ccggcctctg gacgatggcg ctgatgttcg agacggtcag tgttccttct tcgacagcac
    13081 aggtatacgt atttgtgctg ctgtcgaaac gagtctggga gacaaagatc tgctggatgg
    13141 agcggcgcga ctcgaaaccg tcggccttgg cgatgaagct gacgagtcgt ttgcctgggg
    13201 ttgcgttctt gaaattggca t
//
LOCUS       DATIPF010000039         9903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_624166, whole genome shotgun sequence.
ACCESSION   DATIPF010000039 DATIPF010000000
VERSION     DATIPF010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 9903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9903
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624166"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VLL06_01030"
     CDS             complement(<1..410)
                     /locus_tag="VLL06_01030"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HSS29580.1"
                     /translation="MELIQPTRHSHPVGQAPMVKRSLSNRLLLVDPYPRNNPYRLTAS
                     ERRAVWFPKLSLPVIAAYTPEHWEVNLVDEAVQDIDFDFPCDMVGLSIMTCYAPRAYE
                     IATEFRKRGKVVVLGGVHPTYCPDDALRYCDAIV"
     gene            complement(720..986)
                     /locus_tag="VLL06_01035"
     CDS             complement(720..986)
                     /locus_tag="VLL06_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29581.1"
                     /translation="MNMWMLNVTPIVAGMTMGVLMGCGSVGAPIPPENVGVNLTIERQ
                     KKLELLEEKQREAQAEAEATEPQPDSMLQGQDVNLPPLQPVGTR"
     gene            complement(983..2395)
                     /gene="argH"
                     /locus_tag="VLL06_01040"
     CDS             complement(983..2395)
                     /gene="argH"
                     /locus_tag="VLL06_01040"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HSS29582.1"
                     /translation="MGRQSGRPPRRSSSEKAWSGRFRRKTNRLVEAFTVSVAVDRRLY
                     AHDIQGSVAHCKTLAKARVLTASETRTIVQGLESVKMELDRGRFRFTQEDEDIHMAIE
                     RRLTELIGPLGGKLHTGRSRNDQVALDVRLYVRDQLSQLLAHLEQFQRVLVAKGKANR
                     TVAMPGYTHLQRAQPVLLAHHLLAYVEMIERDKGRLRDASVRVNVMPLGSGALAGTNY
                     PVDRRFTAGLLGFPAVTQNSLDAVSDRDFMIEVASALSITMMHLSRLSEELILWSSQE
                     FQFVELPDAFCTGSSMMPQKKNPDVPELVRGKTGRVYGHLVNLLTTLKALPLSYNRDL
                     QEDKPALFDALDTVVASVEVLTELMRRLTVNRDVLKRAVQGGAMLATEVADYLVTRGV
                     PFREAHAITGRLVRAALDQSRELTDFSLEDLRAFSDRIEKGLFLRLTVTAAIDRKSQI
                     GGTARARVERRIQELERMLS"
     gene            complement(2395..3606)
                     /locus_tag="VLL06_01045"
     CDS             complement(2395..3606)
                     /locus_tag="VLL06_01045"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HSS29583.1"
                     /translation="MNCVIKKVVLAYSGGLDTSVILKWLQETYRCEVIAFCADLGQGE
                     DLKAVKLKAQRLGVKRVYVEDLREVFVKDYVFPMLRANAVYEGSYLLGTSIARPLIAR
                     RQIEIAKKEGAEAVSHGATGKGNDQVRFELTYMALEPKIKIIAPWRDWAFRSRRELIE
                     YAEQHDIPVTATKAKPYSMDLNLFHVSYEGGILEDPWNAPPDGIFQMTVSPEQAPDKP
                     QEIEIEYAGGDPVMVNGKKMSPATLLARLNMLGGAHGIGRVDLVENRYVGMKSRGVYE
                     TPGGTILHAAHRGLESLTMDREVLHLRDSLIPRYAELVYYGYWFSPERAMLQTTMDEA
                     QKDVSGTVRVKLYKGNCTVVGRKSKNSLYRLDIATFEEDEVYRQKDAEGFIRLNALRL
                     AIRAQRRKASS"
     gene            complement(3603..4574)
                     /gene="argF"
                     /locus_tag="VLL06_01050"
     CDS             complement(3603..4574)
                     /gene="argF"
                     /locus_tag="VLL06_01050"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HSS29584.1"
                     /translation="MTTRTTGIRAGKRDLLDVAAIGKAEVERLLTSAALLKDKQQRGV
                     PHPLLPGKTLGLLFQKPSTRTRVSFEAAMNQLGGHALVLPMADIQLSRGESVADTARV
                     LSRYLDAIVVRTYDHAIMEEWAREATMPVINGLTDLSHPCQALSDLLTIRERKGRLKG
                     VKIAYIGDGNNVANSLIEAAAKMGMIITLGCPVGYQPEQHVVDRARLEAAKTGAVIEI
                     GHDPNIAAKEADVVYTDVWISMGREREHARRLKVLAPYQVNGRLMHRAKPDAIVMHCL
                     PAHRGEEISAEVLDGPQSVIIDQAENRLHMQKAILAALLGKRSRTRP"
     gene            complement(4606..5802)
                     /locus_tag="VLL06_01055"
     CDS             complement(4606..5802)
                     /locus_tag="VLL06_01055"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HSS29585.1"
                     /translation="MATEELKQDAARYLMQTYTRQPLSIVRGRGMKVYDLAGREYLDF
                     VGGIAVNILGHGHPDLVHAIQHQAAQLIHTSNLYYTEPQVKLGQMLVDHSFADKVFFC
                     NGGAEANEAAIKLARRYSHDKYGAGRFEIITMKNSFHGRTMATLTATGQDKVQKGYEP
                     LLPGFSYVAFNNVEELEQAVTDKTAAIMLEPIQGEGGVFAADRRYLKQVRDLCTQKDV
                     LLIFDEVQTGMGRTGTLFAYEQLGVHPDIMTLAKALGGGVPIGACLATDAVAKVFSPG
                     SHASTFGGNPLACAAGLAVCRALLEGHVLDHARRMGEYFSKGLSDCQSRHRVVREVRG
                     IGLLQGLEVDMDAKAVVSECLARGILINATGEHVLRFVPPLIITELEIDRLLDTLAQI
                     FSAQAS"
     gene            5945..6586
                     /locus_tag="VLL06_01060"
     CDS             5945..6586
                     /locus_tag="VLL06_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29586.1"
                     /translation="MKSCAQCQQQNQEDAKFCYQCGNTLTVEPEAPIAPSVIPVIHAD
                     EHLWRQFIGPHADRYMQHFNKFGLGEQPKFALTWNWPAFLYISFLWFLYRKMYVYALV
                     YALGPMISTYLTGDITVGLIWSIMAGATANYIYYWHCREHIGQIKNGSWTEPAKQDEV
                     LKEAGGVQPYIIYVGIALYVLFIFTTIKMVQDGPPDGDPSPEKPAKPVATSSV"
     gene            6961..7566
                     /locus_tag="VLL06_01065"
     CDS             6961..7566
                     /locus_tag="VLL06_01065"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HSS29587.1"
                     /translation="MARLVLLRHGESQWNLENRFTGWVDVPLSPRGIQEAANAGETLR
                     GLTFDRAFTSVLVRANDTLRLVLDAIGQSNIPIEKDKALNERMYGELQGLNKDETAKK
                     YGEAQVKIWRRSYDVRPPGGESLKDTAERVLPYYEQTIKPALLKGETILVAAHGNSLR
                     ALIMELEQLSREQVLELNIPTGAPLLYELDQTGKVVDHRYL"
     gene            complement(7589..8083)
                     /locus_tag="VLL06_01070"
     CDS             complement(7589..8083)
                     /locus_tag="VLL06_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29588.1"
                     /translation="MSSNSMTPKQAAAALIAAMPVGVSVEQLEEYGIDATEEQARAIT
                     QEVLSLNLFWILAAIEAHIPQKYQSALSELILEILEAGWGTTTPVGSSAWTDYLNAWQ
                     ARRRRYSRLVEDGMSPLAVSAEAAALMEENKLVTEAERRNLLTLLIDFVPVDTYGRLL
                     EDVG"
     gene            8185..8754
                     /locus_tag="VLL06_01075"
     CDS             8185..8754
                     /locus_tag="VLL06_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29589.1"
                     /translation="MFRQIATTCSRWICTAVLLAILVVGAVVFAPRAQADENRWGFGS
                     GLGFTSGTVNGTVLNLAFSGDYFLDRNFSVGPMMQISPMGDLFQVSFAGVGRYYFRLD
                     NGINVVPLMGFGLIHADLDRGVGPGRVDRNDTSWYIPLGASLEYQAAHNLALTSTLIF
                     NLHNIDLSPSLPEKDRTSVALLFGFRFGP"
     gene            complement(8918..>9903)
                     /locus_tag="VLL06_01080"
     CDS             complement(8918..>9903)
                     /locus_tag="VLL06_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HSS29590.1"
                     /translation="LVPPDPLNGQDVTGAEWKTEYDVVTTLRKLGHDVKPLGVKSDLG
                     VLRSAIEDWKPHIAFNLLEEFDGVAVYDQNVVSYLELLHVPYTGCNPRGLMLARDKVL
                     SKKLFSFHRIPFPDFMVVPQGRAVKRPKWLSFPLIVKSVTEEASLGISQASIVQDDDK
                     LKERVEFIHTNIGTGALIERYIEGRELYVGVMGNGHVRVLPVWELIMDKLPDDARRIA
                     TERVKWSRAYQDKYGIRSGEARNLPEGKAQEIQHLAKRVYRALGLSGYARIDVRMDPE
                     GHVYVLEANPNPQIAHDEDFSDSAEKAGYTYKDLLQELLNIGLRWRPAKVA"
BASE COUNT         2121 a         3024 c         2545 g         2213 t
ORIGIN      
        1 cagacgattg cgtcgcaata ccgaagcgca tcgtccggac agtatgtagg atgaacgccc
       61 cccaagacga cgaccttgcc tcgcttgcga aattctgttg cgatctcgta ggcacgaggg
      121 gcatagcagg tcatgatgga caggccaacc atgtcacagg gaaagtcaaa atcgatatcc
      181 tgaaccgctt catcgaccag attgacttcc caatgctccg gcgtataggc cgcaatcacc
      241 ggcaagctca gcttgggaaa ccataccgcg cgccgttccg acgcggtcaa ccgatagggg
      301 ttattgcgag gatacggatc gaccagcaac agccggttgc tcagcgaacg cttgaccatt
      361 ggtgcttgcc caacggggtg gctatgccgc gtaggttgaa taagctccat gaatcgatcc
      421 tccttccctc tggacggcta ttcggctgag gtgctatgcg tcattgaaat gttctgagcg
      481 gctccctctg ggtgatttac actgccttct tcgtcatatc ggaatgcggc tccgtgtgct
      541 cgacctcgaa gcgctcaatc cgacgaggcg tgtttgtata gccttgtatc tgttctcatc
      601 gccaggttct ttcgtatctc tatggtgaag agtctacgga aggagacagg cctgaatatt
      661 caaatggcgc agtatagcgt aatccgtccg cacaggtcca accactgtcc gcctgaacat
      721 caccgcgtac ccaccggttg taacggaggg agattgacat cctgtccctg gagcattgag
      781 tctggttgtg gctcggtcgc ttcagcttcc gcctgggcct cgcgctgttt ttcctcgagc
      841 aactccaact tcttttgccg ttcaatggtc aaattcacac ccacattttc aggaggaatt
      901 ggcgccccca cagatccgca acccatcagg actcccatcg tcatacccgc cacaatgggt
      961 gtcacattca gcatccacat gttcatgaga gcatccgctc gagttcttga atacgccgtt
     1021 cgactcgcgc cctggccgta ccgcctatct gagacttcct gtcgatcgcg gcggtgaccg
     1081 tcaatcgtaa gaacagaccc ttttcaatcc gatcggaaaa tgcccgcaga tcctcgagcg
     1141 aaaagtccgt gagttcacgg ctttgatcca atgcagcacg caccagccgg cccgtgatcg
     1201 catgtgcttc gcgaaacggt acgcctcggg tgacaagata gtccgcaacc tccgtggcca
     1261 gcatcgcacc gccttgaacc gctcgcttga gcacatcccg gttcacagtg agtcggcgca
     1321 tcagttccgt caagacctct accgatgcga ccacggtatc gagggcgtcg aagagcgccg
     1381 gcttatcctc ctgcagatct cggttataac tcaagggcag ggccttgagc gtggtcaaca
     1441 gattgactaa atggccgtag acacgtcccg tcttgccccg caccaattcc ggcacatcag
     1501 gattcttctt ttgcggcatc atactgctac cggtacagaa cgcatcggga agctcgacga
     1561 attggaactc ctgggacgac cagaggatca attcttcact tagccgggac aggtgcatca
     1621 tggtgatcga cagcgcggag gccacctcga tcatgaaatc tcggtcggac acggcatcga
     1681 gactgttttg cgtcacggcg gggaacccca acagcccagc ggtgaaccgg cgatcgactg
     1741 ggtagttggt tcccgcgagc gcaccagatc ctaaaggcat gacattcaca cgcaccgatg
     1801 cgtcgcgcaa tcgtcccttg tcccgctcga tcatctccac ataggccagg agatggtgcg
     1861 ccaacaatac cggctgggca cgctggagat gcgtgtaacc cggcatggca accgttcgat
     1921 tcgccttccc cttcgcgacc aagacccgct ggaactgttc cagatgcgcc aggagttgac
     1981 tcagctgatc ccgcacatag agtcggacat ccagcgcaac ctgatcgttg cggcttcgtc
     2041 ccgtgtgcaa cttgccgcca agcggaccaa tcaattctgt caggcggcgc tcaatcgcca
     2101 tatggatgtc ttcatcctcc tgggtgaaac gaaagcggcc ccgatccagc tccatcttca
     2161 ccgactccaa accctgcacg atcgtcctcg tttcggaggc agtcaacacc cgtgctttcg
     2221 ccaatgtctt gcaatgggcg acactaccct gaatatcatg ggcgtacaac cggcgatcaa
     2281 ctgccaccga cacggtgaac gcttcgacga gccgattcgt cttacgccga aaccgtccgc
     2341 tccatgcctt ctcgcttgat gaacgcctcg gcggacgtcc gctttgtcgc cccattacga
     2401 agaagccttt cgccgttggg ctctgatcgc cagacggagt gcattcaacc ggataaagcc
     2461 ttcggcatct ttttgccgat agacctcgtc ttcttcaaac gtcgcaatgt cgagacggta
     2521 cagtgaattc tttgacttgc gtccgaccac cgtacagttg cccttgtaca acttcacccg
     2581 cacagtcccc gacacatcct tctgcgcctc atccatcgtc gtctggagca tcgcccgttc
     2641 cgggctaaac caatacccgt aatagaccaa ctccgcgtag cgtggaatca aactgtcccg
     2701 aagatgcagc acctcgcgat ccatcgtcag cgactcgagc ccacggtggg cggcatgcag
     2761 gattgtcccg cccggtgttt cgtagacccc gcgcgacttc attcccacgt atcgattctc
     2821 gaccagatcg acgcggccga tcccatgtgc gccacccagc atgttgaggc gcgccagcag
     2881 cgtggccgga ctcattttct tcccattgac catcacagga tcgccgccgg catactcgat
     2941 ttcaatctcc tgcggcttgt cgggcgcctg ttccggcgac accgtcattt ggaaaattcc
     3001 atccggcggg gcattccagg gatcttcgag aatgccgccc tcgtaactca catgaaagag
     3061 attcaaatcc atgctgtagg gcttcgcctt cgtcgccgtc acgggaatat catgttgctc
     3121 ggcatattcg atcaactcac gacgcgagcg aaaggcccaa tcacgccagg gggcgatgat
     3181 cttgatcttg ggctccagcg ccatgtaggt caactcgaag cgaacctggt cgttgccctt
     3241 tcccgtcgct ccatgcgata cggcctcggc accttctttc ttggcaatct caatctgcct
     3301 gcgagcgatc aacggacggg cgatggacgt tccgagtagg tacgagccct cgtacaccgc
     3361 attggcgcgc agcatcggaa acacataatc tttgacaaac acttcgcgca ggtcttcaac
     3421 gtagacacgt ttcacaccga gtctctgcgc cttgagcttg acggccttga ggtcttcccc
     3481 ctgtcccaag tcggcacaaa acgcaatgac ctcgcaccga taggtctctt gcagccactt
     3541 caagatcacg gacgtatcga gcccgcctga ataggccagc accactttct taatgacaca
     3601 attcatggtc tcgttcgact cctcttccct aatagggccg ctaaaatggc cttctgcata
     3661 tgcaaccgat tctcagcctg gtcgatgatc accgactgcg gaccatccag cacctcggcg
     3721 ctgatttctt caccccggtg ggctggcaaa cagtgcatga caatcgcatc gggcttggca
     3781 cgatgcatca ggcggccgtt gacttgatat ggcgccaaga ctttgagtct tcgcgcatgc
     3841 tcccgctcac gccccatact gatccagacg tcggtataga cgacatccgc ttctttcgcc
     3901 gcaatgtttg gatcatgtcc tatctcgatc acggctccgg tttttgccgc ctcgagcctc
     3961 gccctatcga cgacatgctg ctccggctga taccccaccg gacaaccgag tgtgatgatc
     4021 attcccatct tcgccgccgc ctcgatcagg gaatttgcca cattgttgcc gtcaccgata
     4081 taggcgatct tcaccccctt caaccggccc ttcctctccc gaatggtcag gagatctgag
     4141 agggcctgac aaggatggct caggtcggtc aacccattga tcaccggcat cgtcgcttcc
     4201 ctcgcccatt cctccatgat ggcgtggtca taggtgcgga cgacgatcgc gtcgagataa
     4261 cgtgacaaca cgcgcgccgt atcggcaaca ctttctccac gtgagagctg gatatcggcc
     4321 atgggcagaa ccagagcgtg gccgcccaac tgattcatgg ccgcctcgaa cgacaccctc
     4381 gtcctcgtcg atggtttctg gaacagcagc cccaacgtct tcccgggaag caggggatga
     4441 ggaacccccc gctgctgctt gtctttcagc agagccgccg acgtcagcag acgctccacc
     4501 tccgccttgc cgatggccgc aacatccagt aagtctcttt tgccggcccg gattcctgtt
     4561 gtgcgcgtgg tcattcctgt tgtatccatt cggtatgggt acgctttacg acgcctgcgc
     4621 actaaaaatc tgtgcaagcg tatcgagcaa tcgatcgatt tccagctcgg tgatgatgag
     4681 aggcggcacg aatcgcagca catgctcgcc tgtcgcattg atcagaatcc ctcgggccaa
     4741 acactcgctc acgacggctt tcgcatccat atcgacttca agcccctgca agagcccgat
     4801 gccgcgcacc tctcgcacca cccggtgtcg agattgacag tcggataacc ccttagaaaa
     4861 atactctccc atccggcgcg catgatcgag tacgtggcct tccagcaggg cacgacagac
     4921 ggccaagccc gccgcacagg ccaatggatt gccgccgaac gtcgacgcat gactgccggg
     4981 cgagaagacc ttcgcaacag cgtccgttgc aaggcatgct ccaatgggca ccccgccccc
     5041 caacgctttc gccaaggtca tgatgtccgg atgcacccct aactgttcat aggcaaagag
     5101 cgtcccggtc cgtcccattc cagtctgcac ctcatcaaaa atcaagagca cgtctttttg
     5161 agtgcagaga tccctcacct gcttcaaata ccgacggtcc gctgcgaaga cgcccccctc
     5221 accctgaata ggctcgagca taatcgccgc ggtcttgtcg gtcacagcct gttccaattc
     5281 ctccacatta ttgaaggcca cgtagctgaa gcccggcagt aacggctcat agcccttctg
     5341 aaccttgtcc tggccggtcg ccgtcaacgt cgccatcgtc cggccatgga aggagttctt
     5401 catcgtgatg atctcgaacc gcccggctcc atatttgtca tgagagtagc ggcgtgcaag
     5461 cttgatcgcc gcttcattcg cctctgctcc accgttgcaa aaaaacactt tgtcggcaaa
     5521 ggaatggtcc accagcatct gccccagctt cacttgtggc tcagtgtaat agaggttcga
     5581 ggtatggatc agctgcgcgg cttggtgctg gattgcatga accagatcag ggtggccgtg
     5641 tccgaggatg ttcacggcaa ttccgccgac gaaatcgagg tactctcgcc ccgcaaggtc
     5701 gtacactttc atcccacgcc cgcgcacgat cgaaagtggt tgacgcgtat acgtctgcat
     5761 cagataacgt gcggcgtcct gcttcagctc ttccgtcgcc ataatagacg tatccctttg
     5821 taaagtaagg gaaggtagca taggcctgtc gatggaagca acttgaaatg agggcagagg
     5881 gtgagaggcg gagtcgccgt gcttctgtac cagggggtgt ctttattgtg ctattctttg
     5941 gtggatgaaa tcctgcgcgc agtgccaaca gcagaatcag gaagacgcaa aattctgcta
     6001 tcaatgcggc aacacgctga ccgtcgagcc tgaagcacca atcgccccct ctgtgatccc
     6061 cgtgatccac gcagatgaac atctctggcg ccaattcatc ggcccccacg ccgatcgcta
     6121 tatgcagcat tttaataagt tcggattagg ggaacagccg aagttcgcgt tgacgtggaa
     6181 ttggccggcc tttctctata tttcctttct ctggtttctc tatcgaaaaa tgtacgtcta
     6241 tgcgttggtc tatgcactcg gtccgatgat ttcaacctat ttgaccggcg acatcaccgt
     6301 aggtctgatc tggagtatca tggcgggagc caccgccaac tatatttact actggcactg
     6361 ccgtgaacac atcgggcaga tcaagaacgg gtcgtggacc gagccagcga aacaagacga
     6421 agtgctgaag gaagctggag gcgtccagcc ctatatcatc tacgtcggca tcgccctcta
     6481 cgtgctgttc atttttacca cgatcaaaat ggttcaggac ggaccaccag acggagatcc
     6541 ttcaccagaa aaaccggcga agcccgttgc gacaagcagt gtgtgacaag cggcaacgac
     6601 agcagtccat gctatcggaa cgcacggtcc gtagagcaat catgcacacc tgacatgatg
     6661 atgccgttcc agctgattcc cttgtcgaaa aactccttcg acggtcagtg cctgtccatc
     6721 gcagacacgc gaacgagaca cggcgcgaca ttcggacgac tcttctgtat tgcctgtcat
     6781 caacttgtta cgcagggctg tttcggtcaa ccgattcgcg gaggcatatc cgatgctgac
     6841 tgcgtgatgt ggcatcatgc gattccgtga atcacttccc tgctctttca cttcgtatga
     6901 tgatcgttta tcctgtgacg ggttgctcga ccttgttagg tgatcacgga ggcgttaggt
     6961 atggctcgat tagtcctgct ccgtcatggt gaatcgcaat ggaacttgga aaatcgtttc
     7021 accggctggg tcgatgtgcc cctgtcaccg cgaggcatac aggaggccgc gaacgcggga
     7081 gagacactcc ggggcttgac gttcgatcgt gcgtttacct ctgtgctcgt gcgcgcaaac
     7141 gacacgctcc ggctggtact cgacgcgatc ggtcagtcga acattcccat cgagaaggat
     7201 aaggccctaa atgaacggat gtacggcgag ttacagggat tgaacaagga cgagaccgcg
     7261 aaaaaatacg gcgaggcaca ggtgaagatc tggcggcgga gttatgacgt gcgtccgccg
     7321 gggggggaaa gcctgaaaga cacggcggaa cgtgtgctgc cctactatga acagacgatc
     7381 aagcccgcac tcttgaaagg ggaaacgatc ctcgttgcag cgcatggaaa cagcttacgg
     7441 gctttgatca tggagctgga gcagctttcc cgagaacaag tgctggaatt gaatattcca
     7501 acaggtgcgc cgctgctgta tgaactggat caaactggga aggtcgtcga tcaccgctac
     7561 ctctgacaaa tcgcggccgg tcgtgagact atccgacgtc ttccagtaat ctcccatagg
     7621 tgtcgaccgg tacaaaatcg atcagtaggg tcaagagatt gcgccgctcc gcttctgtga
     7681 ccaatttgtt ctcttccatc aatgcggcag cctccgcact taccgcaagg gggctcatcc
     7741 cgtcctcaac caaacgacta taccgacgac gacgtgcctg ccatgcgttg aggtagtcgg
     7801 tccaggcaga tgaaccgacg ggagtggtcg ttccccatcc ggcctccagt atctccaaaa
     7861 taagctctga cagcgcggac tgatatttct gaggaatatg tgcctcgatg gctgccaaga
     7921 tccaaaacag attcagcgag agcacttctt gcgtgatcgc ccgagcctgc tcctccgtcg
     7981 cgtcaattcc atattcttcc agctgctcaa cggacacgcc gaccggcatc gctgcaatca
     8041 gcgcagccgc tgcttgcttt ggggtcatcg agttgcttga catacctcac gctctccctt
     8101 gtcgcagtgg cgagagtacc ctactgctgc tagtcagctc aagcaacttg tcccggcctg
     8161 cctcctgtgc tacagtccgc cggcatgttt cgacaaattg ccaccacatg ttcacgatgg
     8221 atctgtaccg ccgttctgtt ggctatttta gtcgttggcg ccgttgtctt cgcacctcgg
     8281 gctcaagccg acgaaaaccg ctggggattc ggcagtggcc ttggattcac atccggaacg
     8341 gtcaatggca cggtcctgaa cctggccttc agtggtgact actttctcga tcgaaacttc
     8401 tctgtcggac cgatgatgca gatcagtccg atgggcgatc tcttccaagt ctccttcgcc
     8461 ggagtcggtc gctattattt tcgcctcgat aacggcatca acgtggtgcc cttaatgggt
     8521 ttcggattaa tccacgcaga cctagaccgc ggagtcggcc ccgggagagt cgaccgcaac
     8581 gatacgagct ggtacatccc tcttggcgcc tcactggagt accaggccgc ccacaatctc
     8641 gccttgactt cgacattgat cttcaacctc cataatatcg atctctctcc atcacttcca
     8701 gaaaaggacc gcacgagtgt cgccctgctg ttcggtttcc gttttggtcc ttagtcggac
     8761 gagaggaaaa ccgctcgcat cgttcgtgac cctgtctcct catcaacgtt ttcccaacac
     8821 ccgcagccac tacccaggca cggtacccga gccctgtgag gatgctgcac gctgataggc
     8881 tgcagaagtc attgaggatg tttggagagg gtggcagcta cgccactttg gccggccgcc
     8941 agcgaagccc gatgttcagc agttcttgta gcagatcttt atatgtatag ccggctttct
     9001 cggccgaatc agaaaaatct tcgtcgtggg caatttgcgg attgggatta gcttccagca
     9061 catatacgtg cccttccgga tccattctga cgtcgatgcg tgcatagcca ctcagaccaa
     9121 gtgcacgata gacacgcttt gccagatgct gaatctcctg ggctttccct tcgggaagat
     9181 tcctggcttc gcctgaccga atcccatatt tatcttgata cgcgcgactc cacttgactc
     9241 gttctgtcgc aatccgtcgg gcgtcatccg gaagcttgtc catgatcaac tcccacactg
     9301 gcaagacacg cacatgccca ttgcccatca ccccgacgta caattcacgc ccttcgatgt
     9361 accgctcaat gagggcaccg gtcccgatgt tcgtatggat gaattcaact cgctccttga
     9421 gtttgtcatc gtcctggaca atagaggcct gagagattcc gagcgacgcc tcttcggtca
     9481 ccgatttgac aatcaagggg aatgacagcc atttgggccg tttgaccgct cgcccctgcg
     9541 gcaccaccat aaaatccggg aacgggattc ggtggaacga gaacagtttt ttggagagga
     9601 ccttgtcgcg cgcaagcatc aacccacgcg ggttacagcc ggtatagggc acgtgaagca
     9661 attcaagata ggacacgaca ttctggtcgt acaccgccac cccgtcgaac tcttcgagca
     9721 gattgaaggc gatgtgtggc ttccagtctt ctatcgccga tcgaagcacc ccgagatcac
     9781 tcttgacccc cagcggcttg acgtcatgcc cgagcttgcg gagcgtcgtg accacatcat
     9841 attccgtctt ccactcagca ccggtcacgt cttgcccatt gaggggatct ggcggcacca
     9901 gat
//
LOCUS       DATIPF010000040         3644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1685382, whole genome shotgun sequence.
ACCESSION   DATIPF010000040 DATIPF010000000
VERSION     DATIPF010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685382"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="VLL06_01085"
     CDS             complement(<1..1204)
                     /locus_tag="VLL06_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HSS29591.1"
                     /translation="MQPLRALEELSFDNSYARLPEVCYAKVNPTPFAVPPYLVSFNPA
                     AAELIDLDPAQARRPEFAGVFGGGLLVPGMEPLAMLYSGHQFGVYVPQLGDGRAILLG
                     EVRNERGEKWDLHLKGAGLTPFSRDGDGRAVLRSTIREYLCSEAMHGLGIPTTRALCL
                     VGSDHKVYREQVETGAMLVRMAPSHVRFGTFEVFYYRKQHEQLKILADYVIANHFSHL
                     VDGQDKYTRFFSEVVERTAKLIAQWQAVGWAHGVMNTDNMSIHGITLDYGPFGFIDDY
                     DPGFICNHSDHNGRYAFNQQPYIGLWNLSCLAQALLPLASKENLKAVLDRYTPLCERR
                     YMELMQAKFGLSGEQEDDAGLIQDLLSLMQQSHVDYTNCFRTLGSFSSENGSHNEPLR
                     DFFLDREAF"
     gene            1909..>3644
                     /locus_tag="VLL06_01090"
     CDS             1909..>3644
                     /locus_tag="VLL06_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HSS29592.1"
                     /translation="MDASLEKMEKPENGVKGLKHWKYDLMAGLQVAMMGIPLSLGIAI
                     ASGAPPVCGLISAIIAGFIFPFLGGAYITISGPAAGLAPALLAGMILLGDGNLEVGYP
                     LLLVAICLVGVVQVILSIFDAGRFALYFPISVVEGMLMAIGMLIIVKQVPAFLGVIIP
                     PIKSIPKAIISVPEQIMVLNPLICAIGAATLILLFVITSILSRIKAAWAKFIPVPLVV
                     IAMAGITAWFIGVEEKYLIHVPSDIFEHGIVFPKFTEAFTRTELYWPLLLTIITLTLI
                     DGTESLATIQAIDKIDPFKRKSNPNVTLRAMGVSNTASSLLGGLTIIPGGMKSTTNML
                     VGGRTLWANFYYAVFQLIFLLFATALINQIPKSALAGLLMWVGWKLCSHKVFQRIFAV
                     GKEQMFIAMVNVVVTLWTSDLLEGMIVGTLTKIVLLVKDVVKASPGGGIGAGLKELFG
                     NPIIRIGDGAGSRDVMTVSANVVLRGTMGEMKNPYKIYLSSITCMNLVKLDAALKKIV
                     VPEGAKANYMLILAGKIIDHTTMEYLHNFQDRTIEAGHTCVLVGTDRFRGLSDHALAY
                     RVKAQESAIAFA"
BASE COUNT          823 a          987 c          997 g          837 t
ORIGIN      
        1 taaatgcctc acgatcgaga aagaagtcac ggagcggttc gttgtgcgat ccattctcgg
       61 atgaaaagga tcccagggtc cggaaacagt ttgtgtaatc gacatggctc tgctgcatga
      121 gtgacaagag atcttggatc aacccagcat catcctcctg ttccccgctc aaaccaaatt
      181 tggcttgcat cagttccatg tagcgacgct cgcagagggg ggtgtagcga tccagtacgg
      241 ccttgaggtt ctctttcgat gcgagcggta acagcgcctg cgccaggcag ctgagattcc
      301 agagaccgat atagggctgt tgattgaatg cataccggcc gttgtggtcg gagtggttac
      361 agatgaaacc cggatcgtag tcgtcgatga acccgaacgg accgtagtcg agggtgatgc
      421 catggatcga catattgtcg gtgttcataa ccccatgggc ccagccgacg gcctgccatt
      481 gtgcaatcag ctttgcggta cgctcgacga cctcggaaaa gaatcgtgtg tacttatcct
      541 ggccatccac tagatgggaa aagtggttcg caatgacata gtcggcaagg atcttgagct
      601 gctcgtgctg tttacggtaa tagaagacct cgaaggtgcc gaatcgcaca tgagatggtg
      661 ccatgcgcac aagcatcgca ccggtttcga cctgctcgcg atagacctta tggtcgctcc
      721 cgacgagaca gagcgcccga gtcgtcggaa tccccaggcc atgcatcgct tcactgcaga
      781 gatactcacg gatagtcgaa cgcagaacgg cacggccatc gccatcacga gaaaacggtg
      841 tcaacccggc acctttcaaa tgcagatccc acttttcgcc ccgttcgttc cgtacttctc
      901 cgaggaggat cgcccgcccg tcccccaact gggggacata gaccccaaat tgatggccgg
      961 agtagagcat ggccaacggt tccatccccg ggactagaag acccccacca aacaccccgg
     1021 caaactccgg tcgcctcgct tgcgccggat caaggtcgat cagttcggct gctgcgggat
     1081 taaaactgac gagatacgga ggcacggcaa acggcgtggg gttcaccttg gcatagcata
     1141 cctccggcag gcgtgcgtag ctgttgtcga atgacaattc ttccagtgcg cgcaagggtt
     1201 gcatcccttc atctgtagca cggcacggcc aggaatcgct aggcggtacg tgatcaacgg
     1261 gggactccgt aagagaccag cgtcggttgt ggaaaataga tcgacggtca catccaaaca
     1321 tttgcatcta atgcggcgca aaggacctgt acgggaacaa cagtgccctg tggtggattg
     1381 gaaagatgat gggccggtcc aacctatgcg gatctggtcc agcgagattc tataaacaca
     1441 actatgctga actatactga tggggccaat cggttagtat gagcgattgt gcactgtgcg
     1501 cacagtgcga gttcgctcat atataaatcg atcactttcg cacaccccta ttgcctatga
     1561 tccatatttt catttaagat gccgtattta caggcattaa tgtggcattg ccaagcttct
     1621 gtatgtgttt ggtgcgaggc ttctctgatg gcatcggttg tgcgtagacc ctcagcccac
     1681 tataacggga gattgttacg gctctgggcc tacccgagtc atcacggcgt gattgatcag
     1741 gatcagatcg gatgtgactg catgatgaga gatctcaacg ccagcgggac aatgaatcaa
     1801 cgtctctgtc tgagaaagca caacacatgt atcgtttcaa cgcatcacta gggaaacgat
     1861 aaattcagga ggaacagcat gtcgtcgcat ggatcacaaa cgctagcaat ggatgcttca
     1921 ctcgagaaaa tggagaaacc cgagaacggg gtgaaggggc tgaagcattg gaagtacgac
     1981 ttgatggcgg gcttgcaagt cgccatgatg gggatcccgc tctcgctcgg gattgcgatc
     2041 gcctccgggg ccccgccggt ctgcggcctg atctcggcga tcatcgccgg atttatcttc
     2101 cccttcctcg gtggcgccta tatcacgatc agcggtccgg ccgccggcct ggcaccggca
     2161 ctgttagccg gcatgatctt gctgggcgat ggaaatttgg aagtgggcta cccgctcttg
     2221 ctggtggcta tttgtctcgt cggggtcgtc caggtgatct taagtatctt cgacgccggg
     2281 agattcgcac tatactttcc catctcggtg gtcgaaggca tgttgatggc gatcggcatg
     2341 ctgatcatcg tcaagcaagt cccagccttt ctcggggtga tcatcccgcc catcaagtcc
     2401 attcccaaag ccatcatttc tgttcccgaa cagatcatgg tgttaaatcc gttgatctgc
     2461 gccattggag ccgcgacatt aatcctcctc tttgttataa cgagcatcct gtcccgtatc
     2521 aaagcagcct gggcgaagtt cattccggtt cccctcgtcg tgatcgccat ggctggcata
     2581 accgcgtggt tcatcggggt cgaggagaaa tacctcattc acgtgcccag cgatattttt
     2641 gaacatggga ttgtcttccc aaaatttact gaagccttta ccagaacaga gttatactgg
     2701 ccgctcttac tcaccattat cacgctgacc ctcatcgacg gcaccgagtc gctcgcgacg
     2761 attcaagcca tcgataaaat cgatcccttt aaacgcaagt cgaaccccaa tgtcaccttg
     2821 cgggccatgg gcgtttccaa caccgcttcc agtctgttgg gtggcttgac gatcatcccc
     2881 ggggggatga agagcacgac caacatgctc gtcggcggcc gcaccctctg ggccaacttc
     2941 tattatgcgg tctttcagtt gatcttcctg ttgttcgcca ccgccctcat caatcaaatc
     3001 cccaaatcgg ccctagccgg gttgctcatg tgggtcggtt ggaaactctg ctcgcacaaa
     3061 gtcttccagc ggatcttcgc ggtgggtaag gaacagatgt ttatcgccat ggtcaacgtc
     3121 gtggtgacgt tgtggacctc ggacctgttg gaaggcatga tcgtcggcac gctcacgaag
     3181 atcgtcttgc tcgtgaagga tgtcgtcaag gcgtccccgg gtggtgggat aggcgccgga
     3241 ttgaaagaat tgttcggcaa tcccattatc cgaatcggag acggcgctgg gtctcgtgat
     3301 gtgatgaccg tgtcggcgaa cgtggtgtta cggggcacca tgggcgagat gaagaatccc
     3361 tacaagatct acctctcgtc aatcacctgc atgaatctgg tgaaacttga tgcggcgctc
     3421 aagaaaatcg tggtgcctga aggggccaaa gccaactata tgcttatttt ggcgggaaag
     3481 attatcgacc acaccacgat ggaatatttg cacaatttcc aggatcgcac catcgaagcc
     3541 gggcatacct gtgtcctggt cgggacagat cgtttccgtg gcctctcgga tcacgcactg
     3601 gcctatcgcg tcaaagccca ggaaagcgcc atcgcgttcg catg
//
LOCUS       DATIPF010000041         4311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1597196, whole genome shotgun sequence.
ACCESSION   DATIPF010000041 DATIPF010000000
VERSION     DATIPF010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597196"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1159)
                     /locus_tag="VLL06_01095"
     CDS             complement(65..1159)
                     /locus_tag="VLL06_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HSS29593.1"
                     /translation="MTIWVRRALVLGGLWAVMTIPIAIGAQPQSEHSETKESMPRFAA
                     NGESERDLILLDPLENPNESEGRLVILPEIKREGERYFLSSFKLPDKLTFAGQPVPLD
                     NWQVRERIEYEFYQFLEDQGESIILAKRTGRCFPPAERQLAEAGLPDDLKYMLLVESK
                     CVAAAYSKAKASGPWQFIPSTGRRYRLKSDAVRDERRNLEMSTEAAVKYLKYLKDFRE
                     NDWFMAMASYNAGEERVRKLLKEQKITDYWKMHGPRETMRYVPRIIAAKEIYSQPEKY
                     LGLSKKDLYMPMETETVTVNVKEPQRALTSIAEEFGTYFLELKMLNPEFKKDVLPRGS
                     YQMRVPRQTCPSRCFTQDKTPLQDAEKARQ"
     gene            1275..1982
                     /locus_tag="VLL06_01100"
     CDS             1275..1982
                     /locus_tag="VLL06_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HSS29594.1"
                     /translation="MRALTLLNDRITHCTACPRLVTYRETIAAERRKQFESWTYWGRP
                     VPGFGDFHARLYVLGLAPAAHGGNRTGRVFTGDRSGDWLYDALHRFGFANQPNSHHRD
                     DGLKLSDCYIGATVRCAPPDNKPAPEEFTACRPYLREEIRLLRNVRVVVALGKIAFDH
                     YLKACRDEGLQLPSPIPKFSHGIVTVLPWNTTLIGSYHPSQQNTFTGKLTRPMFHHIF
                     ATARQRLAETNHPPADR"
     gene            complement(1985..2632)
                     /gene="fsa"
                     /locus_tag="VLL06_01105"
     CDS             complement(1985..2632)
                     /gene="fsa"
                     /locus_tag="VLL06_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HSS29595.1"
                     /translation="MKFFLDTANVKEIQEAASLGLLDGVTTNPSLVAKEGRIFKEVLI
                     EICNIVDGPISAEVVSLEADAMVKEGKVLAKIHKNIVVKVPLIAEGLKATKRLAAEGI
                     KVNVTLCFSPTQALLAAKAGAWCVSPFIGRLDDISSNGMELIRQILTIYRNYDYKTQV
                     LVASVRHPQHVVEAALAGGHICTMPFAIFQQMVKHPLTDTGLKKFLADWDAQSKK"
     gene            2822..3133
                     /locus_tag="VLL06_01110"
     CDS             2822..3133
                     /locus_tag="VLL06_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29596.1"
                     /translation="MMQRSAWIFLMCLALIGCGGNPYLEQSLKPAELQGKDKAWFEKN
                     WGAPSGKASRFFGGETWTYYRIAGGKSGLPFFNFSPNQCQITLKFDKEEKLSDSSYSG
                     C"
     gene            complement(3130..3381)
                     /locus_tag="VLL06_01115"
     CDS             complement(3130..3381)
                     /locus_tag="VLL06_01115"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HSS29597.1"
                     /translation="MYRTILLVGLLVVLYFLLRQAFRGLKGRSEPDLLAPGKDHMVQD
                     PVCKVFVPRGTAITEDIGGQTYYFCSQSCAHKFQEQLAG"
     gene            complement(4032..>4311)
                     /locus_tag="VLL06_01120"
     CDS             complement(4032..>4311)
                     /locus_tag="VLL06_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HSS29598.1"
                     /translation="NAVNRDLDQVGVYTRKGLIGERKKGEIGIQTIRAGDIVGDHTIL
                     FGGMGERIEVTHRASSRDTFARGALRAARWVVRQPPGLYDMMDVLGLR"
BASE COUNT          966 a         1230 c         1085 g         1030 t
ORIGIN      
        1 agtgaaatac tactgttgtg ctcgagaact gtgatggtac gcggcagtcc atcacaggag
       61 aacgttactg gcgggctttt tcagcatctt gcaaaggagt cttgtcttgc gtgaagcaac
      121 ggctcggaca ggtctgtcgc gggactctca tctgatagct tccgcgtggg agcacgtcct
      181 tcttgaattc aggattcagc atcttgagtt ccaagaagta ggttccaaat tcttcggcaa
      241 tcgaggtcag ggctcgctgg ggctccttca cattgacggt gaccgtttca gtttccatcg
      301 gcatatacag gtctttctta ctcagtccca agtacttctc cggctgtgaa tagatttcct
      361 tggcggcaat gatcctcggc acatagcgca tggtttcccg tggcccatgc atcttccaat
      421 agtcggtgat cttttgttct ttgaggagct tgcgtacccg ctcttcacca gcattgtagg
      481 aggccatggc cataaaccag tcgttctctc tgaagtcctt gagatacttg aggtacttaa
      541 ctgccgcctc ggtggacatc tccaagttcc gtcgttcgtc ccgtaccgca tcgctcttga
      601 gacgatagcg tcgcccggta gaaggaatga attgccatgg ccccgacgcc ttggccttcg
      661 aataggcggc agcgacacac ttgctctcca ccagcaacat atacttgagg tcgtccggta
      721 acccggcttc tgccagctgc ctttcggcgg gagggaaaca gcgaccggtg cgtttggcaa
      781 gaataatact ctcgccctgg tcctctaaga attgatagaa ctcatactca attcgttcac
      841 gtacctgcca gttatcgagc ggaaccggtt ggccggcaaa cgtcagcttg tcaggtagct
      901 tgaacgagct gaggaagtag cgctctccct cccgtttgat ttcaggaagt atgacgagac
      961 ggccctccga ttcgttggga ttctccaggg gatcaagcag gatcagatca cgctccgact
     1021 ctccattggc tgcaaaccga ggcatactct ccttggtctc agaatgctcc gactgcggct
     1081 gtgctcctat cgctatcgga atcgtcatga ccgcccacaa cccaccgagt accagtgctc
     1141 gtcgaaccca tatcgtcatg ctgatcctgc ctcctccatg agtgaacgat ccatgtcgaa
     1201 tacgcatgct aacagccaag caagggagcg gcaagtatga tcgactttcg catccatgtt
     1261 agactgcgcc agctatgcgt gcgctgaccc tcctcaacga tcgcatcacc cactgcaccg
     1321 cctgtccacg gctcgtcacc tatcgggaga cgatcgccgc agagaggcgg aagcagtttg
     1381 agagctggac ctactggggc cgtccggttc ctggattcgg agacttccat gcgcgactct
     1441 atgtgcttgg cctggcaccc gctgcccatg gcggcaatcg aaccggacgg gtatttactg
     1501 gcgatcggag cggagactgg ctgtatgacg cgttacatcg attcggcttc gccaaccaac
     1561 ccaactctca ccaccgggat gatggactga aattgtcgga ttgttacatt ggcgcgaccg
     1621 tccgttgtgc gccgccagac aataagccgg cccctgaaga atttacagcc tgccgcccct
     1681 atctgcgtga agagatccgg ttgttgcgga atgtccgagt ggttgtggca ttgggtaaga
     1741 ttgcgttcga tcactacttg aaagcttgcc gtgatgaggg actgcaactc ccctcaccaa
     1801 tcccgaaatt ttcccatgga atcgtcacgg tccttccgtg gaacacgaca ctgatcggtt
     1861 cataccatcc cagccagcaa aataccttta ccgggaaact caccagaccg atgtttcacc
     1921 acatctttgc gacggctcgg caacgacttg ccgagacaaa tcacccacca gccgaccgct
     1981 agccttactt cttggattga gcatcccaat ccgccaggaa cttcttgagt ccggtatccg
     2041 tcagcggatg tttcaccatc tgctggaaaa tcgcgaaggg catcgtacag atatgtccac
     2101 ccgctagcgc agcttcgacg acatgctgag gatggcgcac gctggccacc agcacctggg
     2161 ttttatagtc gtaattgcgg tagatggtca gaatctggcg gatcaactcc atcccgtttg
     2221 aactgatatc gtcaagacga ccgatgaatg gtgagacgca ccaggcacca gcttttgctg
     2281 caaggagcgc ctgggtcgga gaaaagcaca acgtgacatt caccttgatt ccctcggcgg
     2341 ccagtcgttt ggtagccttc aaaccctcgg caatcaatgg taccttcacg acgatgttct
     2401 tatggatctt tgcaagcacc ttcccttcct tcaccatggc atcggcttcg agactcacga
     2461 cttctgcgct gatcggccca tccacgatgt tgcaaatctc gatgagcact tccttaaaga
     2521 tgcgtccttc cttggcaaca agcgacggat tcgtggtcac tccatcaagt agtccgaggc
     2581 tggctgcctc ctggatttcc ttcacattgg ctgtatcaag aaagaatttc atgggcttct
     2641 cctttcacgt gggaatgcag aatccttgtg atccattcat tatcggttat tctatgaaga
     2701 actcaacctc ggtgcaatct gccgtcgatc tgtgtcagtt tgacaggtcc cagactgccg
     2761 gatagaattc gaatattcaa gactcatctc tggctgtatc gataggatcc gaggagactg
     2821 catgatgcaa cgatcggctt ggattttcct catgtgtctg gccctgatcg gctgcggcgg
     2881 taatccctat cttgaacaat cgctcaaacc ggcagaactc caaggcaagg acaaggcttg
     2941 gtttgagaag aactggggag ccccgagcgg caaggcctcg agatttttcg gcggtgaaac
     3001 ctggacctac tatcgcatcg ccggtggcaa atcaggtctt cccttcttta acttctcccc
     3061 taatcagtgc cagattacgc tcaaattcga caaggaagag aaactctccg actccagtta
     3121 ctcaggctgt taaccggcaa gttgttcctg aaacttgtgt gcgcaggatt ggctgcaaaa
     3181 gtaataggtc tgaccgccaa tatcttctgt aatcgccgtt ccccttggca caaatacctt
     3241 gcagaccgga tcctgcacca tgtgatcttt cccaggagca aggaggtccg gctcgctccg
     3301 acctttcagc cctcgaaaag cctgccggag gagaaagtac agcaccacaa ggagcccgac
     3361 aaggaggata gttctataca tggtgggggc ctcttcctga taccacaatc ctatgggacg
     3421 atacctctac tgtcaaggac ctcataatct gttttggttg gatcaacgaa cacatcctca
     3481 ttctcccccc gccctctaac ggcctagctg cctatgagcg gaagggccta cttcccaaga
     3541 agaaccagta cttcaggtgg atatgcttct attctttcgc atggtaggtt gtcgtcatga
     3601 cgatttgtga taaatgccgc ggagcgatgt tgccggaacg ggcagtggac ctgaacgcag
     3661 ggcttgcgat cacagtattt gcttgtttga attgtggtcg caggaaatcg gcggaccagg
     3721 aacctcggcc tattaccgca agacattaac cgtcgcgacg atagaaccga atccgccttc
     3781 caaagatttc agcttagtac tgattggatg gagtccctcc caagaaatcc tgcctgtccc
     3841 tctgcgtcaa tgactacccg aagcaccatg gatttcgacc accaagcagg aagaatcttc
     3901 tagtcccctc cgtatctctc caagtgcatg gctgatatga gcctgctcac ggtttagcag
     3961 aggacctgcc accacagcag atacgtgcgg ggcagattca tgaaatgggg aatcggagca
     4021 gggaagagga actatcgtaa acccagcacg tccatcatgt cgtacaggcc cggcggctgc
     4081 cgaaccaccc atcgagccgc acgcaaggcg cctcgtgcaa aagtatctcg gctgcttgct
     4141 cgatgggtca cttcgatgcg ttcacccatc ccgccaaaga gaatcgtgtg gtcgccgaca
     4201 atatcgccgg cacggatggt ttgaattccg atctcgccct tcttccgttc cccgatgaga
     4261 ccctttctcg tatagacacc gacctgatcg agatcgcggt tcaccgcgtt t
//
LOCUS       DATIPF010000042         4055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_711925, whole genome shotgun sequence.
ACCESSION   DATIPF010000042 DATIPF010000000
VERSION     DATIPF010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711925"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="VLL06_01125"
     CDS             complement(<1..143)
                     /locus_tag="VLL06_01125"
                     /inference="COORDINATES: protein motif:HMM:NF045310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HSS29599.1"
                     /translation="MAVDQQGLLTRLVRELVRLPGIGQKTAQRLAFHILKAERDDALR
                     LAEA"
     gene            complement(157..480)
                     /locus_tag="VLL06_01130"
     CDS             complement(157..480)
                     /locus_tag="VLL06_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HSS29600.1"
                     /translation="MKNPLGNMGNILKQAQAMQAKMAKVQEEASTKTVTGTAGGGSVA
                     VTANGAMQLVGIVIDPEVVKSGDVEMVQDLVLAASNDALKKAREMMAEEMKSVTGGMN
                     VPGMF"
     gene            complement(477..2315)
                     /gene="dnaX"
                     /locus_tag="VLL06_01135"
     CDS             complement(477..2315)
                     /gene="dnaX"
                     /locus_tag="VLL06_01135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HSS29601.1"
                     /translation="MDYQVSARKYRPGTFDDVIGQPHVVQTLVNSIATKRIAQAYLFS
                     GTRGVGKTTVARILAKALNCEQGPTGTPCGTCSNCLEIAQGTSVDVMEIDGASNTSVD
                     DVREIRENVKFAAFRGKYRVYIIDEVHMLSNSAFNALLKTLEEPPPHVVFIFATTEIH
                     KIPATILSRCQHYNFRRIARTEIVERLRHVVDQDKIVIEERSLMALARASEGSMRDGL
                     SLLDQAVAFGGKTIVHADLEALLGAVPQELVRAMIQAITAQESAAALRVLAQLLDQGH
                     DLRAYCAEVVEYLRNMLVVSVVTVPKEREGLIEVSTEDLAQMATDARSFSPELLQELL
                     SIFTQAEDSLRFSAHPRFVLETAAVRATRLLRRSDEKPPQAVQPAQTTRPPQSAPTHT
                     GQPVSPVRSANPMLSSPPRPLQSTAPALTAPMPSKSSVVGSTEASRGLPTNSPIVRSP
                     GTAGEVKASIPVASSLTDDRPVAVTLNWEQVQEEVANASSRIAAFLERGRCVGVEGHV
                     VTVGFAKQEMAARGMIENPENMAVITSICERLSGKTVRLRIVELTEADPPGPTMAQLR
                     AAKGKEQRLVLFEQARAHPVVRQTLEVFGVELAEVRPLSSQKEVAE"
     gene            complement(2425..2524)
                     /gene="ffs"
                     /locus_tag="VLL06_01140"
     ncRNA           complement(2425..2524)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VLL06_01140"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(2809..2898)
                     /locus_tag="VLL06_01145"
     tRNA            complement(2809..2898)
                     /locus_tag="VLL06_01145"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2862..2864),aa:Ser,seq:gga)
     gene            3047..>4055
                     /locus_tag="VLL06_01150"
     CDS             3047..>4055
                     /locus_tag="VLL06_01150"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HSS29602.1"
                     /translation="MKIVIGILLVVVAVVGVILALPFLIDLNKYQDQYKPLIEDALNR
                     KIQLQDIRLTMWPRIGARVAGFAVLDDPAFGSSPFTSLRSVDVGVKLMPLLSGKIEVE
                     DITLRDPEIAIIKNKNGILNVSTVGRTGIALPTTPSRAPIPSTEGPLKILALLAVDRV
                     SIVGGTLSYRDLSSPKPAEYMFQNFDLLLQSVRLGQTPSLHISTLVQPFNLPVTLDGT
                     FGPLKETTDIDAIHVQLTVEKTVFTITGSTVGHDAKVNVTSPAINTANFPMALPLQKP
                     VELKNVEIAAAVKGQEVNLSQLSFRLFDGEVTAQGSLTSGSESPPFTGKALVCNCGGG
                     FG"
BASE COUNT          870 a         1233 c         1048 g          904 t
ORIGIN      
        1 gcctcggcca ggcgaagcgc gtcatcgcgt tcggccttca agatatggaa ggccaagcgc
       61 tgcgccgttt tttggccgat gcccggtaga cggactaact cgcgcaccag ccttgtgagt
      121 aatccttgtt gatctacagc catgacgtga tgcctgctag aacattccag gaacgttcat
      181 cccaccggtg accgatttca tctcttccgc catcatctca cgagccttct tgagggcatc
      241 gttcgatgca gccaagacca aatcttgtac catttccacg tcgccacttt tcacaacttc
      301 gggatcaatt acgatgccga caagctgcat agctccgttg gccgtgaccg ccacactgcc
      361 gccgccggcc gtcccggtta ccgtcttggt cgaggcctct tcttgcacct ttgccatttt
      421 cgcttgcatg gcctgcgctt gcttcagaat attccccata ttgcctaacg ggttcttcat
      481 tccgcgacct ccttctggct cgacagcggt ctcacctctg caagctcaac accgaacacc
      541 tccaacgtct gcctgacgac cggatgtgcc cgcgcttgct cgaagaggac cagccgctgt
      601 tcttttcctt tcgccgccct gagctgtgcc atcgttggtc ctgggggatc cgcctccgtc
      661 aattcgacaa tccgcagccg aacggtcttc ccgctcagtc gttcacaaat cgatgtgatc
      721 actgccatat tttcaggatt ctcgatcatc cctcgggctg ccatctcctg cttggcaaaa
      781 ccaactgtca caacatggcc ttccacaccg acacaccgcc ctcgttccaa gaaggcagca
      841 atccgagaag acgcattggc cacttcctct tggacctgtt cccaattaag tgtcaccgca
      901 acaggtctat catccgtcaa agacgatgcc acaggtatcg atgcctttac ctccccggca
      961 gtgccaggag atcgaacaat cggagaattc gtcggaagac ctcgtgaagc ctcggtactc
     1021 ccgacaacag aggacttcga cggcatcgga gcagtcagtg ctggagccgt tgactgaaga
     1081 ggccgcggtg gcgaggaaag catcggattt gcagaacgaa ccggcgagac cggctggcca
     1141 gtatgcgttg gagcagactg aggcgggcgt gtcgtctggg ctggttgtac cgcttgaggt
     1201 ggcttctcat ccgatcgacg cagcaaccga gttgcgcgca cggctgccgt ttcgagcacg
     1261 aaccgaggat gggcactgaa cctgagcgag tcctccgctt gcgtgaagat cgacaacaat
     1321 tcctgaagta gctcgggcga gaacgagcgt gcatcggttg ccatctgtgc gagatcttcc
     1381 gtggagacct cgatcaaccc ctcacgctct ttgggtacag tcaccaccga taccaccagc
     1441 atgttgcgca gatactcgac aacttccgca caataggccc gcagatcatg cccttggtcc
     1501 aacaattgtg ccaacacgcg aagcgccgca gcgctctcct gagccgtgat cgcttgaatc
     1561 atggcacgaa caagttcttg cgggaccgct cccagcaagg cttccaaatc cgcatgcacg
     1621 atcgtcttgc caccgaacgc caccgcctgg tcgagcaggc tcaatccatc gcgcatgctg
     1681 ccttcgctgg ccctggcaag cgccatcagg cttcgttcct cgatcacgat cttgtcctga
     1741 tcgaccacat ggcgtagccg ttcaacgatc tcggttcgcg caatccggcg aaagttgtaa
     1801 tgctgacagc gggacagaat cgtcgccgga atcttgtgaa tctcggtggt ggcgaagata
     1861 aacaccacat gcgggggcgg ctcctccagc gttttcaata gcgcgttgaa agccgagttc
     1921 gacaacatgt ggacttcgtc gataatgtag actcggtact ttccacgaaa agcagcgaac
     1981 ttgacgttct cgcggatctc ccgtacatcg tcgacgctcg tattggacgc cccatcgatc
     2041 tccatgacat cgacggaggt cccctgcgca atttcgaggc agttggaaca ggtcccgcag
     2101 ggtgtaccgg tcggtccttg ctcgcaattc agtgccttcg caagaattct tgcgactgtg
     2161 gttttcccca ccccgcgggt tccggagaag agataggctt gggcaattcg tttcgtagcg
     2221 atggaattga ccagcgtctg aacaacatgt ggctgcccga tcacgtcgtc aaacgtgccc
     2281 ggccggtact ttctggcaga tacttggtaa tccataaata acgttaggcg taaggggtaa
     2341 ggcgtgaagg cgtgagatga atgactccgg aaatccgtat caccttacgt tgcatgtttt
     2401 tatccctcac ggataaagat taagcggggc caggtgatcc tggggcacac agaactgacc
     2461 gcttaccgtt gcttccttcc ggacctggcg gggttcacag ggttctgtcg cacagggccc
     2521 agccccttat ttcaacgtga aaagtaaaaa gggaaaagta gggagtgcat atgaccgaaa
     2581 cttttgcctt ttaacttctt acttttacct cgccttggtg tggcggagag ggtgggcatt
     2641 cgaagtcgcg cgcgctgaaa cagcgcctgc tcctaacaga gggggccagc cccctcgtat
     2701 gctccccacg cgggggatac atcccccacg atccccctgt tcgaatccca ccctctccat
     2761 ttttcatctc tcccgagctt ccccgactca tccctcaatg cctttttgtg gcggagaggg
     2821 tgggattcga acccacggtc ccattgctgg gacgcatgct ttccaagcat gtcgattcgt
     2881 ccactctcgc acctctccgc accgattttc gcgaggcggc atcttaacac gcgatctgat
     2941 gaccggcaag gtgaagaaac agcatccaca cagggaagcg ctgactcaca ttgaccaagg
     3001 tcgagcccct tcctatactg aatcgagttc atagaaaggt acgctgatga aaatcgtgat
     3061 cggtatactt cttgtcgtag tcgcggtggt cggagtcatc ctggcacttc ccttcctgat
     3121 tgacctcaac aagtatcaag accaatacaa gccactcatc gaagatgccc tcaatcgcaa
     3181 gattcagctg caagacatcc gactgacgat gtggccgcgg attggtgccc gagtagccgg
     3241 gtttgctgtg ctggatgacc cggcattcgg ctcaagcccc tttacctctc tgagatcggt
     3301 ggacgtgggg gtcaaactca tgccgttact cagtggcaag atagaggtag aagacatcac
     3361 gctccgtgac cccgagatcg cgatcatcaa gaacaaaaat ggcatcctca acgtctcgac
     3421 cgtcggccgc acaggtattg cgctgccgac cactccttcg cgtgcaccga ttccgtcgac
     3481 cgaaggtccg ctcaagatcc tggcgctgct ggccgtggat cgagtctcta ttgtcggagg
     3541 aacactgagc taccgtgatc tgtcatcccc taaaccggct gagtatatgt tccagaactt
     3601 cgatttgctg ctccaatccg ttcgtctcgg ccaaactccc agcctgcata tcagtaccct
     3661 tgttcaacca ttcaatctgc ctgtgacgct agacggcaca ttcggccccc tcaaagagac
     3721 gacggatatt gacgccatac atgtgcagct gacagtggag aagaccgtct tcaccatcac
     3781 cggcagcacc gtcggccacg atgcgaaggt caacgtcacc tcgccggcca tcaacaccgc
     3841 gaattttccg atggccctgc cgctgcaaaa accggttgaa ttgaagaacg tcgaaattgc
     3901 agcagcggtc aagggccaag aggtgaatct gagccagctg tcttttcgac tcttcgatgg
     3961 agaggtgaca gcacagggaa gcctcacctc cggatccgag agtcctccct tcaccggcaa
     4021 ggccctggta tgtaattgcg gtggtggctt tggcc
//
LOCUS       DATIPF010000043         2577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1155591, whole genome shotgun sequence.
ACCESSION   DATIPF010000043 DATIPF010000000
VERSION     DATIPF010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155591"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1121
                     /gene="ruvB"
                     /locus_tag="VLL06_01155"
     CDS             114..1121
                     /gene="ruvB"
                     /locus_tag="VLL06_01155"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HSS29603.1"
                     /translation="MVERVVSVQITDEERSIETVLRPQTLEEYVGQERMKESLRICIE
                     AAKQRNEALDHTIFYGPPGLGKTTIAHIIAREMGASLRSTSGLVLAHAGDLAAILTNL
                     QEHDVLFIDEIHRLPASVEEALYPAMEDYQLDLVIGQGPAARTVKLDLPRFTLVGATT
                     RAGALTSPLRDRFGLVHRLEFYSPAELETIVTRSARVLGISITSEGAREIAGRARGTP
                     RIVNRLIKRVRDYAQVKASGQITQTVAEDALAWLGVDTAGFDEMDRQILLTIIDKFAG
                     GPVGVESLAAAVHEDKGTLEDVHEPYLIQAGFLERTGRGRQATKLAFDHFKKPISPLF
                     S"
     gene            1313..2389
                     /gene="pheA"
                     /locus_tag="VLL06_01160"
     CDS             1313..2389
                     /gene="pheA"
                     /locus_tag="VLL06_01160"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HSS29604.1"
                     /translation="MSGDFSEYRKEIDRIDDEILRLLNERSKSVVEIGKIKKQRDADA
                     NLHTPAREAAIIERLIRQNSGPFPSEAIRPVYREIMSASLSLEGPQKVAYLGPRATFT
                     HMACMQKFGSSAQYFPVNSIKEVFSEVERGRANFGVVPIENTTEGVVTHTLDMFIDSD
                     LLIYGEILQEVSHHLMSKSGVMEEVKKVHSHPHAIAQCRNWLEANLPNVPLSEVASTA
                     RAAELCVENAFVAAIASELAAQLYGLKVIKARIEDNMNNMTRFLILSQKPPERTGKDK
                     TSLMLSVKDKVGALYDLLRPFASLGLSLTKIESRPSRRKAWEYIFFVDIEGHIQEERV
                     KKAVEEVTGRCLFMKVLGSYPAHS"
     gene            2394..>2577
                     /locus_tag="VLL06_01165"
     CDS             2394..>2577
                     /locus_tag="VLL06_01165"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247023.1"
                     /note="catalyzes the formation of L-histidinol phosphate
                     from imidazole-acetol phosphate and glutamate in histidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HSS29605.1"
                     /translation="MPLKIHPDIASLNPYVPGKPIEELQRELGLARVIKLASNENPLG
                     SSPKALAALTEVIATLH"
BASE COUNT          589 a          705 c          723 g          560 t
ORIGIN      
        1 cgacggattg tggtagtacg gcaacgccag ggcaaggtgc cataccgcct ccacccaagt
       61 atctcccaac gccggagaag aaataagacc agaacgaaag gggctcccac acgatggttg
      121 agcgagttgt cagcgtgcag attacggacg aagagcgaag tatcgaaacg gttctgcgtc
      181 cgcagacctt ggaggagtat gttggccagg aacggatgaa agaatccctg cggatctgca
      241 tcgaggcggc gaagcagcgg aacgaagcac tcgatcacac gatcttctat ggtcctcctg
      301 gattggggaa gaccaccatt gcccacatca ttgctcgtga gatgggggct tcgctccgtt
      361 cgacctccgg tctcgtcctg gcccatgcag gcgatttagc cgccatcctg acgaatctcc
      421 aagaacacga tgtcctcttc atcgacgaga ttcatcgact tcctgcttct gtcgaggagg
      481 cgctctatcc agcgatggag gactatcagc tcgatctcgt gatcggccag gggccagcgg
      541 ccagaacggt gaaattggat cttccccgat ttacgctggt tggagcgacg accagggctg
      601 gggccctgac ttccccactc cgggaccgat ttggcctcgt ccaccgactg gagttctatt
      661 ctccagcgga gttggagacc atcgtgacac gctctgcgcg ggtactcggg atttcgatca
      721 catccgaagg cgcacgagaa attgctggcc gtgcgcgagg aaccccacgc atcgtgaatc
      781 ggctgatcaa gcgggtgcgc gactatgctc aggtcaaggc gtcgggacag attacccaga
      841 ctgtcgccga ggacgcactt gcctggctgg gtgtcgatac agcggggttt gatgaaatgg
      901 accgtcagat acttttgact atcatcgaca agtttgctgg aggaccggtc ggggtggaat
      961 ctctggcggc tgcggttcat gaagataagg gcaccctcga agatgtccat gagccctacc
     1021 tgattcaggc cggatttctt gaacgaaccg gccgtggccg ccaagccaca aagctggcct
     1081 tcgatcattt caaaaagccg atctctcccc tcttttcgtg atgaatccga actccgttca
     1141 gacgtctgat cgcaggtgtg tactgacggg gatggtcacg caagctcgtg aaacgtcaag
     1201 ggtctccttg cattggtagg aatggttcct gtatcatttc ccacttggcc tgtgccactt
     1261 cagccgatgg agaggtcacg aattctatcc gctggaaaga gggggaggag ctgtgtccgg
     1321 agacttttcc gagtaccgga aagaaatcga tcgcatcgat gacgaaattt tgcgcttact
     1381 gaacgagcga tccaaaagcg tcgtcgagat cggcaagatc aagaaacagc gggatgcgga
     1441 tgccaatctc cataccccag cgcgggaggc ggcgatcatt gagcgtctta tccgacagaa
     1501 ctccgggccg tttccctcag aggcgattcg gccggtgtat cgagaaatca tgtctgcgtc
     1561 tctctctctc gaaggtcctc agaaggtggc gtatcttggc cccagagcga cgtttacgca
     1621 tatggcctgc atgcagaaat tcgggtcgtc cgcccagtat tttccggtca atagcatcaa
     1681 ggaagtattc agtgaagttg agcgggggcg agccaatttt ggagtagtcc caatcgagaa
     1741 taccaccgaa ggcgtggtga cccacacgct cgatatgttc attgattccg atctcctgat
     1801 ctatggcgaa attctccagg aggtgtctca tcatttgatg tcgaaatccg gcgtgatgga
     1861 agaggtcaaa aaagtccatt ctcatcccca tgccattgcc caatgccgaa attggttgga
     1921 agcgaacttg cccaatgtgc cgttgtcgga ggtagcgagc accgcacgtg ccgcggaact
     1981 gtgcgtggaa aatgcctttg ttgccgcgat tgcatcggag ttggcggctc agctgtacgg
     2041 cctgaaggtc atcaaagccc gcatcgaaga taatatgaac aacatgaccc gttttctcat
     2101 cctgtcgcaa aagccgcccg agcgtacagg aaaagataag acttccttga tgctctcggt
     2161 gaaagacaaa gttggtgccc tgtacgatct cctccgacct tttgcctcgc tcggcctcag
     2221 cctgaccaag atcgaatcgc ggccctccag aagaaaagcc tgggagtaca ttttcttcgt
     2281 cgacatcgaa gggcatattc aggaagagcg ggtcaagaaa gccgtcgaag aagtcactgg
     2341 ccgatgtctg ttcatgaaag tcctcgggtc ctacccggcc cacagttgat gccatgccac
     2401 tgaagatcca tccagatatt gcctcactga atccctatgt tcccgggaaa cccattgagg
     2461 aacttcaacg ggaactgggt cttgcccgcg tcattaagct ggcgtccaac gagaatccgt
     2521 tgggctcctc tccgaaggcg cttgccgcgt tgaccgaggt aatcgccacc ctccacc
//
LOCUS       DATIPF010000044         8028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_183270, whole genome shotgun sequence.
ACCESSION   DATIPF010000044 DATIPF010000000
VERSION     DATIPF010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8028
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183270"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1578
                     /locus_tag="VLL06_01170"
     CDS             <1..1578
                     /locus_tag="VLL06_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HSS29606.1"
                     /translation="CPYIEYSDRTLVDIELLKNLNVDYLKKNHWMPLKRDRTAIEILT
                     DDPSDLDRVADIKRTFPGLNIRFAISLRRDIAQFLSSATGQGQGDTGGTRKLDENVSD
                     ILGELVSEAQEAAAEEAGGGLDENDNAIVRLANQIIADAYRQGASDIHVEPYGEKRET
                     LVRFRVDGDCFEYMKIPQSYRRAIVSRLKIMASLDIAERRKPQDGKIKFKLSESKEIE
                     LRVATIPTAGYNEDVVMRLLAASEPLPLDKMGFSDRNLEAIKVIAAKPYGIILCVGPT
                     GSGKTTTLHSVLGFINTPDIKIWTAEDPVEITQYGLRQVQVLPKINFTFAAAMRAFLR
                     ADPDVIMVGEMRDKETAEIGIEASLTGHLVMSTLHTNSAVETVTRLLDMGCDSFSFAD
                     AMLGVLAQRLTRRICKDCKEQYVGTPVEYEEIRQGYGPEQWDKLAIPQDNTFRLVRGK
                     GCETCNRTGFKGRVALHELLLGSDRIKRMIQTKARTEEMLNAAIEDGMTTLMQDGVQK
                     VLLGQTTFKEVKAVAIK"
     gene            complement(2228..2578)
                     /locus_tag="VLL06_01175"
     CDS             complement(2228..2578)
                     /locus_tag="VLL06_01175"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS29607.1"
                     /translation="MNRFRQHPRVRISAPFVCALSHRQSRRWLRRPGIDLGVVYDLSI
                     GGARVSTEAEIRLGDEVMLSLRLPRQITRADIAVATVQWTKDQFFGLAFTELSPAAQN
                     RLKKYVTNASTTAA"
     gene            3405..3620
                     /locus_tag="VLL06_01180"
     CDS             3405..3620
                     /locus_tag="VLL06_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29608.1"
                     /translation="MSRHAKEGIIGRAGKTTIAKRDREKAKQIQRREKDARREQRKTE
                     KPARPSNTEGEDPDLAGLQWGPQEPLY"
     gene            complement(3762..5012)
                     /locus_tag="VLL06_01185"
     CDS             complement(3762..5012)
                     /locus_tag="VLL06_01185"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSS29609.1"
                     /translation="MHTSVHTSFHTLGLSEPLLRDLTAAGLLVPTPIQAQAIPLALEG
                     RDVIGCAQTGTGKTAAFVVPMIERLAVLPKGQPQALILAPTRELALQIFDSIEKLGRS
                     HRISATVIVGGSDMQAQIRGLRQRPDILVATPGRLLDHMWNGTVNLATMKILVLDEAD
                     RMLDMGFAPQINQILDALPVERQTLLFSATLPTDVTRLVQASLNSAVHVMATPSSTTA
                     DGVTQALHHTTSHEKTNLLVALLGSERGTVLVFARTKSRVDRLGQTLEQAGHRVAVIH
                     GDRSLPQRLRALDGFKRGQVRILVATDIAARGIDVANIGHVVNYDLPSCPEDYIHRIG
                     RTARMKMTGRATSFVTSEERDQLRAIETLLGHPVPLAAGSPRPSDRPQSRVSDRSRDD
                     RQRWGRPGEDRRQHQPSLVVPEVG"
     gene            5647..6552
                     /locus_tag="VLL06_01190"
     CDS             5647..6552
                     /locus_tag="VLL06_01190"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HSS29610.1"
                     /translation="MVSQAPMVNVTPIDVPNQIGRRRHTTWTVILFCAITMSAVIGVP
                     LYGYFYHYTWLDWSLFGLLYVVSGLGITVGYHRLSSHRSFDCPNWVKGTLLIAGAWAL
                     QNSAIKWASDHLRHHAHCDDEKDPYNAQRGFWHSHCGWLFRSDQYVDQKYATRVANDP
                     VAIWQHKYYPLLVLTGLASTFLIGFLHNGIMGGVGCFLLAGVGRTFAVLNSTFCINSI
                     CHLWGRQPHGTSDSSRDSWLVSLVTFGEGYHNYHHMYPTDFRNGPCWYHFDPSKWLIL
                     GLYSLGLARSLRTGSFVEESHARTN"
     gene            6574..6837
                     /locus_tag="VLL06_01195"
     CDS             6574..6837
                     /locus_tag="VLL06_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSS29611.1"
                     /translation="MGKRVLYVGGLPDKVSDCELRGIFTSYGLVARAYVVRFKHTGKS
                     AGYGFVEMGSGEQALNAVVALEGTEWEGHLLRLYVTPYANQTV"
     gene            6868..7170
                     /locus_tag="VLL06_01200"
     CDS             6868..7170
                     /locus_tag="VLL06_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29612.1"
                     /translation="MGRTNLDPIMTFPDGSHLLISTACSKEGNFSCALYMATIAADDQ
                     GAFRIVSNHLAAATCLVAQEDAYSYAQRLYPRSAETMKKPPYLIWPGPGPTGNADV"
     gene            7497..7832
                     /locus_tag="VLL06_01205"
     CDS             7497..7832
                     /locus_tag="VLL06_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSS29613.1"
                     /translation="MGSKLYVGGLPYAATESQLTTLFAAHGTVESARVITDKFTGQSR
                     GFGFVEMATPEEAKAAITALNGSQMDGRPLTVNEAKPQEPRTGGGGGGGGRFGGGGGG
                     GDRGGRGRF"
BASE COUNT         1963 a         2325 c         2068 g         1672 t
ORIGIN      
        1 tgcccctaca tcgagtacag cgatcgaaca cttgtcgata tcgaacttct taaaaacctg
       61 aatgtcgact acctcaagaa aaatcactgg atgcccctca agcgggaccg aaccgccatc
      121 gaaattttga cggacgaccc gagcgatctc gatcgagtag cggatattaa acggaccttc
      181 cccggcctga acattcgatt tgcgatcagt ctccgtcgcg atatcgccca gttcctcagc
      241 tccgccacag ggcaaggaca gggcgatacc ggcggcacac gaaagttaga tgaaaatgtc
      301 tccgacattc tcggcgaact tgtcagcgaa gcccaagagg cggcggcgga ggaggccggt
      361 ggggggctcg acgaaaacga caatgccatc gtacggctgg ccaatcagat catcgccgat
      421 gcctaccgac aaggcgcgtc cgatatccac gtcgaaccct atggcgaaaa gcgtgaaacg
      481 ctcgtccgct tccgggtcga cggcgactgt ttcgaataca tgaagattcc gcaaagctat
      541 cggcgcgcca ttgtgtcgcg tctgaaaatc atggccagcc tcgacatcgc agaacggcgc
      601 aaacctcagg acggcaagat caaatttaaa ctgtcggaga gcaaggaaat cgaattgcgc
      661 gtcgcgacaa ttccgaccgc cggatataac gaagacgtgg tgatgcgcct acttgcggcc
      721 agcgagccgt tgcccctcga caagatggga ttctcggacc ggaatctcga agccattaag
      781 gtgatcgccg caaaacccta cggcatcatt ctctgtgtcg gaccaaccgg atcgggaaaa
      841 accacgacct tgcactccgt cctcgggttt atcaacacgc cagacattaa gatctggaca
      901 gctgaagacc ccgttgaaat cacgcagtat gggctcaggc aagttcaggt tctgccaaag
      961 atcaacttca catttgccgc tgccatgcgc gcgtttctcc gcgcagaccc tgacgtgatt
     1021 atggtcgggg aaatgcgaga taaagaaacg gctgaaatcg gcattgaagc ttcattgacc
     1081 ggacacctcg tcatgagcac actacacaca aacagcgcag tagaaaccgt cactcggctt
     1141 ctcgatatgg gatgcgattc gtttagtttt gccgatgcga tgttgggagt cctcgcccag
     1201 cgcctcaccc gcaggatttg caaggactgt aaagagcagt acgtcgggac accggtagaa
     1261 tacgaggaaa tacgccaggg ctatggacct gaacaatggg acaaactcgc cattccacaa
     1321 gacaatacgt tccgcctggt ccgcggaaaa ggatgtgaga cctgcaatcg caccggattt
     1381 aaaggacgcg tcgccctgca tgagttgctt ctgggatcgg atagaattaa acgcatgatt
     1441 cagacaaagg cgaggactga agagatgctc aatgctgcca ttgaagatgg tatgaccact
     1501 ctcatgcaag atggggttca gaaggtcttg ctaggccaga cgaccttcaa agaagtgaag
     1561 gctgtggcca tcaagtaacc tgtggcaaaa gcacagctcg cgccagaaga cttcaacctc
     1621 acgacgtcct ctcccctctt cggctatctc ccctgcaatc gtgcaagcct cagcctgtcc
     1681 atacaattag atgtgtctca ccctccccca cttgatcgag agaaaaagac atatcggtcc
     1741 tgccaggcac tttggcacat gaaaggccca ccattttctc tcatgtatga cgcgaactct
     1801 tccaacaggc ttcatgaaag aagggcctct gcttgtggcg atggcacgag agaaagacgc
     1861 gtccgcatat gggtttcagc cggttcagag tatgtgacgg tcgcaaaatc gcgccgacat
     1921 gcattagcac aatctgatct cgaaaccaca aacggatcct cagagttcat ggaggcctga
     1981 ctcatacttg tacgaagggt gccagaacac caacctgcag gtgcctcctc aggtcgatcg
     2041 tcatcctggg cctctcttcg ggcatccttc attcggtttt agccccggct aaacgaatac
     2101 cccacacgta actgccgcat caataatcct tccccaagct gatatttaga atctaccagc
     2161 ggcagcgcag caccttcttt caagtcaatc acgcaatggt ggtacgacac agggatgagc
     2221 gaggatgcta tgcggcggtc gttgaagcgt tcgtcacgta ctttttgagg cgattctgtg
     2281 cagcaggaga taattcggtg aacgccaggc cgaagaactg atccttcgtc cactgcacgg
     2341 tggcgacggc aatatcggca cgcgtgattt gtctaggaag gcgtaggctc aacatgactt
     2401 catcacctag cctgatctct gcctcggtac tgactcgggc gccgcctatc gaaagatcgt
     2461 agaccacgcc caaatcgata cccggcctcc tcaaccaacg acgagactgg cgatgagaga
     2521 gggcgcagac aaacggagcc gagatgcgga cccgtggatg ctggcgaaac ctattcaaga
     2581 cagaagatcg atgcgaggtc tgttccattg tgaaccccaa tcatgtttct accgtacaac
     2641 aggacgctcc attcacaaat acaaatcctc aaacgcacca atcgaattgg ttcatgcagt
     2701 aaacattcat ccgcagatcg atacaccaca cacgagcaat cgttcgagga cagttgcgtc
     2761 aacgcagatc gacaggcatg tcttgcattc aaatttatgc aattagctaa ataccctatt
     2821 tgactcttac catggtccat ggtaatttgt gcagtcgtat gagttaacat tgtccctcgc
     2881 gcccatctag acgcacgcga tcatctcagc gcgatcgaac ttgcagcaga tgttgctcaa
     2941 acgttccatt tatttcttgc actcgaacat ctggcaggaa cgtgggtgga atgatttggg
     3001 cctttcattc cgcccccccc tctccatcga gcatactctc atgttggaga tgccaggatt
     3061 tataaaggag cacgtatgaa gctatcccct gggtggcgcg ccgacaagac aagaaaaaac
     3121 cgagcacggg caacacggaa agcgaaagtc cgatgggagg tgctcggcct tcccggtcct
     3181 atagacacca gtctgacaac ctcaatggag gacattcgtc agcaggtgtc gaacatggcc
     3241 agctgtgggt tcggccaagg cactcgttcc cgtacggccg aacgagaagc aaagacccct
     3301 aaccgtgctc ccgactcaag tggaaagaga catcgtgggg cgacggagcc gccgcaaacc
     3361 aagagaaaat aggaccataa acggtaggcg attggcgacc caatatgagt cgccacgcaa
     3421 aggagggcat catcggacga gcaggaaaaa cgaccatcgc aaagcgagac cgcgagaaag
     3481 cgaagcaaat tcagcggcgc gagaaagacg cccgccgaga gcagcgcaag accgagaagc
     3541 ccgctcgtcc ctcaaataca gagggggaag atcctgatct ggcagggctt cagtggggtc
     3601 cacaggagcc gttgtattag ggtttcacgt gagtgcgtcc tcacgcacca cactaaatcc
     3661 ccttttacgg tctccgacac ctttacacac cagctcagcg gccgacatca cctacagttc
     3721 tgggtaaccg tactgcgata ctcgcccttc tgttcagacc cctaacccac ctccggcaca
     3781 acaagactcg gctggtgctg acgtcgatcc tctcctggac gcccccaccg ctgacggtca
     3841 tctctggaac gatcagatac ccttgattgc gggcgatcac tgggacgggg gcttcctgct
     3901 gccaagggca cgggatgccc cagcaatgtc tcgatcgcgc gcagctgatc acgttcctcg
     3961 gatgtcacaa aactggtggc tcgcccggtc atcttcatcc gggctgtccg tccaatacga
     4021 tggatatagt cctccggaca actcggcaaa tcgtaattca ccacgtggcc gatgttcgcc
     4081 acatcaattc ctcgagcagc aatatccgtc gcaacgagaa ttcggacttg ccctcgttta
     4141 aatccgtcaa gagcacggag acgctgaggt agacttcggt ctccatggat gacggcgaca
     4201 cgatgcccgg cctgttcaag cgtctgaccg agtcgatcga cccgactttt tgtccgagca
     4261 aatacgagga cagtccctcg ttccgaccca agcaatgcca ctagcaaatt cgtcttttcg
     4321 tgggaggttg tatgatgcaa cgcctgggtc acaccgtcgg ccgtcgtcga agaaggtgtc
     4381 gccattacat gaacagcgct gttcagactc gcttggacca accgggtaac atccgtcgga
     4441 agagtagcgg aaaagagtaa cgtctgtcgt tccaccggca acgcgtcaag aatctgatta
     4501 atctgcggag caaatcccat atcgagcata cggtctgctt catcaagcac gaggatcttc
     4561 atggtggcca aattgaccgt cccgttccac atgtgatcca gcaaacgccc cggtgtcgca
     4621 accaggatat ccggccgctg gcgcaagcct ctgatctgcg cctgcatgtc gctccctcca
     4681 acaatgacag tggccgagat gcggtggctt cgaccgagct tctcaatcga gtcaaaaatc
     4741 tgcagcgcca actcccgcgt tggagcgaga atcaatgcct gaggctgacc tttcggcagg
     4801 accgccagcc gttcgatcat gggaacaaca aacgcggccg tcttccccgt cccagtttgc
     4861 gcgcaaccga tgacgtctcg gccttcaagg gcgagaggaa tggcctgagc ttgaataggg
     4921 gtgggaacta gtaagcccgc tgccgtcaaa tcccgcaaaa gcggttctga cagaccaagg
     4981 gtatgaaaac ttgtgtgaac agaggtatgc aatgcactat cctttcagtg tgatcgcatg
     5041 aagacgggat cagagaaccg aggggagaga aaccaggaag gcatcaactc cgacaggacc
     5101 tggtcgcgat gcgatcgcga agtccgttat ggtgtataca tccgtgccgt cggactactg
     5161 cgacaacgat gtagtaagta tcgtacagca agcccactct gacgtcaact ctcttgaaaa
     5221 agacagttat catagtgcca tagtgccgag gagagaaaca gagattgttc cttttcaagg
     5281 cattggggga tagtcatgcc ctaccaaacc ggtgcgttcg ctatcctatt gagactgtgc
     5341 tatgctcacc agagatgacg tccggtcact actctatccc tcgcgccttc actctctccc
     5401 tctagattct gcgcgatcga tgctctagtt tccgcctagc tccgcacgca cgtctctata
     5461 gcgagacaac agagtcgcac cagtccgtcc cgccctcggc cctgccacac tcacccgatc
     5521 ggcggacaac aaatgacggt acaggcctga acgaatgaac atgcagcgta tacaaggcgg
     5581 cgcgttgagg ccaaaacacc cataccctgg acaagaggat caatgtccca aactgcacaa
     5641 caacaaatgg tttcacaagc accgatggtc aacgtcactc ccatagatgt ccccaaccag
     5701 attggtcggc gacgccacac gacctggacc gtcatacttt tttgcgcaat taccatgagt
     5761 gcggtgatcg gggtccctct ttatggatat ttttaccact acacctggct ggattggtcg
     5821 ctgtttggac tactctatgt cgttagtggg ctgggcatca cggtcggcta ccaccgattg
     5881 tcgtcccatc ggagctttga ttgtccgaac tgggtcaaag gcacactctt aatcgcaggc
     5941 gcctgggcgc ttcagaattc agccatcaag tgggcgtccg atcatctgcg tcatcacgca
     6001 cactgtgacg atgaaaaaga tccatacaat gcgcaacggg gattctggca tagccactgc
     6061 ggatggctct ttcgctcaga tcagtacgtg gaccaaaaat atgctacgcg cgtcgcgaac
     6121 gaccccgtcg cgatctggca acacaagtac taccccctcc tggtcctcac cggcctggcc
     6181 agtacgttcc tcatcggctt tctccacaat ggcatcatgg gtggagtggg ttgctttctt
     6241 ctcgccggtg tggggcgcac attcgccgtg ctcaattcga cgttctgcat caactccatc
     6301 tgccatctat ggggacgaca gccacacggc acgtcggact ccagcaggga tagctggctt
     6361 gtgtctctcg tgacatttgg cgagggatac cataactacc atcacatgta tccgaccgac
     6421 ttccgaaacg gtccttgctg gtaccacttt gacccctcga aatggctcat tctcgggctc
     6481 tactcactgg ggcttgcacg atcattgcgg acaggctcgt ttgtcgaaga gtcgcacgct
     6541 cggaccaact aatacgtcac gaaggggacc tcgatgggaa aacgggttct gtatgtcgga
     6601 ggactcccgg acaaggtctc ggactgcgag ttacgtggca tcttcacctc ctacggtctg
     6661 gtcgcccgag cctacgtggt ccgattcaaa catacgggaa aatcagccgg atatggattc
     6721 gtcgaaatgg ggtcaggcga acaagcctta aatgccgtag tcgcccttga aggaaccgaa
     6781 tgggagggac acctcctgag gttgtatgtg accccctacg cgaaccaaac cgtctgagat
     6841 catgccagtc acgaaaaagg agtcaccatg ggccgtacca acttggaccc gattatgacc
     6901 tttccagacg gatcacatct cttgatcagc accgcctgtt ccaaggaagg aaacttttcc
     6961 tgtgccctct atatggcgac catcgcagcg gatgatcaag gggctttccg cattgtgtcc
     7021 aaccacctcg ccgcagccac gtgcttggtg gcgcaggaag atgcctacag ttatgcccaa
     7081 cgtctctacc cgcgttcagc tgagacgatg aaaaagccac cctatttgat ctggcccgga
     7141 ccagggccca caggcaatgc ggatgtctag gacggtgact gaccatctcg caccaggcat
     7201 caaccacaat tgtcgcccca cccatatcat tctccctcat tgtgcaggca ccacaaatta
     7261 gacaacaggg actatagctt ttcgcatgcg cctctgttac gatgctcatg tttcacgcgg
     7321 cggacctcgc atgaaacggc aaataaaacg gtccatcacc gatattgttg gtcccgagaa
     7381 tctgatcgga agctcgaccc aagccgctcc attgttcgcg tcttcggcct gttccttccc
     7441 ctcgcgtgtt cgttctcgag tctcatgcaa tatcaatttc agaaggagga acccccatgg
     7501 gttcaaaact ttatgtcggc gggttgccct atgcggcaac tgaatcacag ctcaccaccc
     7561 tcttcgccgc gcacggcacc gtcgagtctg cgcgtgtgat cacggacaag ttcaccgggc
     7621 aatcacgagg cttcggtttc gtcgagatgg ccacaccgga agaagccaag gccgcgatta
     7681 ccgcgttgaa cggctcacag atggacggac gtcctctgac ggtcaatgag gccaaaccac
     7741 aggagccccg caccggcggt ggtggcggtg gtggtggtcg ctttggtggt ggcggtggcg
     7801 gcggagatcg cggcggtcgc ggtcgcttct aagttactgc gcagaatcga cagatacgaa
     7861 aggggcgctt caattgaagc gccccttcac gtcataccac atccctcata cctcaggttc
     7921 aattcgccgt agcattagcc tccggcttcc ataactctac agatcgagac ctcaaaccga
     7981 tgacacccat gtacacatgg gcttcggatg ttgtttcacc tgtgctgt
//
LOCUS       DATIPF010000045         3332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1598333, whole genome shotgun sequence.
ACCESSION   DATIPF010000045 DATIPF010000000
VERSION     DATIPF010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598333"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1603
                     /locus_tag="VLL06_01210"
     CDS             <1..1603
                     /locus_tag="VLL06_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HSS29614.1"
                     /translation="ATAEILNFRRPGAEQAAPYFVEYIRQLLVAKYGEAMVYKGGLKV
                     VTTINMEMQKVAEAAFAVGLRELDKRQGWRGPLRTVDLTAPTPPIVAAVVDQVLKVGD
                     YREGFVTKVGKDQYLVQIGTMQAQLAFDDMAWAKRRLTGPDTAKDVVVNPNLKQVLKP
                     GDVIEVMVKQLDRGLVHVQLEQTPLVEGGLIALEPGRGAIRAMVGGYDFSRSEYNRAV
                     QAHRQPGSAFKPIIYATAVSQGMSPASVILDAPVVYEQEQDEKTWKPENYGRKIHGMV
                     SLRDALAHSHNLATVRLLDKVGVKNVIDFAKTVGITSPLAADLSLGLGSSSLGLMELT
                     SAYSVLLNQGSRAEPYAILFVKDNAGTVLEQAEPQALQVISKETAYLVTNMMEDVIQK
                     GTGQAAKMIGRPIAGKTGTTDEFINAWFIGGAPNLVTGIYVGFDDRRSLGETESGAHA
                     ALPIWINFMQKALKPLPVIAFTIPEGITFVKVDPATGLLEDGQEDQPSTVELFTKGSE
                     PTQTTQRRLDPIDFYKLDQIPDGAL"
     gene            complement(2009..3178)
                     /locus_tag="VLL06_01215"
     CDS             complement(2009..3178)
                     /locus_tag="VLL06_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSS29615.1"
                     /translation="MGAIGRRDFLVRSGLAIGAALLAAEAPVSTANSASPKFDTWQAV
                     REQFPLSRDYIHLAGFFLASHPTSVRAAIERHRRGLDADPIGYWFEQEEKQETAVLQA
                     AAAYLAVDPTEIALTDSTTMGLGLLYGGLTLREGQEILTTLHDHYATETSLRLRAERT
                     GATIRQIPLYHSLKNVSRQELLDNLIKEIRPNTRIVAVTWVHSSTGLKLPIQEMAQAI
                     HKLNASRAEQDRVIFCVDGVHALGVEDFRLSDVGCDFLVAGTHKWMFGPRGTGLVWGQ
                     KRAWPVTNPIIPTFNTQAYDIWMKVSPQKDLPRSVYMTPGGFHSFEHRWALDEAFLFH
                     QAIGKARVTQRIYELNQQLKQGLARMPHITLHTPMSQELSAGIVCFEVAGMQPTK"
BASE COUNT          745 a          869 c          982 g          736 t
ORIGIN      
        1 ggctacagcc gagatactca actttcgtcg tcctggcgcc gaacaggctg cgccctactt
       61 tgtggagtat atccggcagt tgctagttgc aaaatacggc gaagcgatgg tgtacaaggg
      121 cggacttaag gtcgtgacga caataaatat ggagatgcag aaggtcgccg aagcagcctt
      181 tgcggttggc ttgcgcgaat tggataagcg ccagggttgg cgcggtccgc tccgaacggt
      241 cgatctgact gctccgaccc cacccattgt tgctgcggtg gttgatcagg tactaaaagt
      301 aggagactat cgggaagggt tcgtcaccaa agtggggaag gaccagtatc tggtgcagat
      361 tggcacgatg caggcccaac tcgcctttga cgatatggca tgggcgaagc gccgtctcac
      421 cgggccggat acggcgaaag atgtcgtggt caatccgaac ctcaaacaag tgctgaaacc
      481 tggtgatgtg atcgaggtga tggtgaagca gctggatcgt ggcctcgttc atgtgcaact
      541 cgaacagact cccctcgtcg agggagggct gatcgcgctg gagccaggca ggggagccat
      601 tcgggccatg gtcggaggct atgatttcag tcgtagcgag tacaatcggg ctgtgcaagc
      661 ccaccgccag cctggatccg cttttaagcc gattatctat gcgacggctg tgagtcaagg
      721 catgagcccc gcgtcggtga tcctcgatgc gccggttgtc tatgaacagg aacaggatga
      781 gaaaacatgg aagccggaga actatggaag gaaaatccat ggcatggtga gtttgcgtga
      841 tgcgttggcc cactcgcaca atctggcgac ggtccggctg ctggacaagg ttggcgtcaa
      901 gaacgtcatt gattttgcga aaacagtagg gatcaccagt cctttggcgg cagacctgtc
      961 gctaggactg ggctcatcct ccctcgggtt gatggaattg acctctgcct atagcgtgtt
     1021 actcaaccag gggagccgcg cagagccgta tgcgattctc tttgtgaagg ataatgcggg
     1081 aactgtgctg gaacaggctg aaccgcaggc gctacaggtg atttccaaag aaacagccta
     1141 cttagtgacg aatatgatgg aagacgtgat ccaaaagggg accggtcagg cggctaaaat
     1201 gatcgggcga ccgatagcgg ggaaaactgg cacgacggac gagtttatca atgcgtggtt
     1261 tattggcggt gcaccaaact tggttacggg catttatgtc gggtttgatg atcgacgttc
     1321 actcggcgaa acggaatctg gggcgcatgc cgcgctgccc atatggatta actttatgca
     1381 gaaagcgctg aagccattgc cggtcatcgc ctttaccatt ccggaaggca tcacgttcgt
     1441 caaggtcgat ccggcgacgg ggctgcttga ggatggccag gaagaccagc ctagtaccgt
     1501 ggaactcttt accaaaggca gcgaaccgac tcaaacgact cagcgccggc tggacccgat
     1561 tgatttctat aagctcgatc agattcccga tggggcactc tagcaggatg ctgaaaaagt
     1621 ccgccagctt ccttctcgca tggttcagac cctcaacggg acccggccgc ctcaccactc
     1681 ggcggcgcgc acagacgtgg tgctccttat tcgtcgcacc gtgcgcccca gagggtacgc
     1741 ctccggttct tgctcctgcg gccttgctgg acggtctttt tgagcatcct gcgtaatatt
     1801 tctgttgtcc gtattctgcg acgtgcttgc taacgagtag agggcaagat gtcgcatttc
     1861 aattcaattc agacccgcag cgtcagaatc tcctttaatg tctgttcaat atcctctgat
     1921 gagttgagga gacttggcgc caagcgcgca tactgcgtcg cgtagggcga ggtactcccg
     1981 acaatcttcc tgtgccggag ccgttcgatc acttggttgg ttgcatccca gctacttcga
     2041 agcagacgat gccggccgac agctcttgcg acatcggtgt atgcaacgtg atgtgcggca
     2101 ttctcgccaa tccctgcttc aactgctgat tcaattcgta gatgcgttgg gtgacacgcg
     2161 ccttaccgat ggcctgatgg aataggaagg cttcatcaag agcccaccga tgctcgaagg
     2221 aatgaaatcc acccggtgtc atgtagacgg atctcggtag atctttctgc ggactcacct
     2281 tcatccagat atcgtaggct tgagtattga aagtcggaat gatggggttg gtcacaggcc
     2341 aggcccgttt ctgcccccac acaagaccag taccccttgg accaaacatc catttatgtg
     2401 tccctgcaac caggaaatcg cacccgacgt cggagagacg gaaatcttcg acgcccaacg
     2461 catggacccc atctacgcag aagatcactc gatcctgctc agcacgggat gcattcagtt
     2521 tgtgaatcgc ctgggccatc tcctggatcg ggagcttcaa cccggtgctc gaatggaccc
     2581 acgttactgc aacgatacgg gtgttgggtc ggatctcctt gatgagattg tcgagtaatt
     2641 cttggcgcga gacattcttg agggaatgat agagcgggat ctgtcgtatc gtggccccgg
     2701 tgcgttctgc acgaagccgc aaggacgttt cagttgcata atgatcgtgt agggtcgtga
     2761 ggatttcctg accctcacgc agggtgaggc cgccgtataa gagtcccagc cccattgttg
     2821 tgctatcagt gagagcgatc tcggtcggat cgactgcaag ataggctgcc gcggcctgca
     2881 agacggccgt ctcctgcttt tcttcctgtt cgaaccaata gccgatagga tcggcgtcca
     2941 atccgcgccg gtggcgttcg atcgctgctc gaaccgaagt aggatgtgat gcgagaaaga
     3001 atcccgccaa atgaatatag tcacgggaaa gtggaaattg ctcacgtacg gcttgccagg
     3061 tgtcaaactt cggtgacgct gagttggcag tggaaacagg agcctcggca gccagcagtg
     3121 ccgcaccaat ggccaggccg ctccgaacaa gaaaatccct ccggccaatc gcacccatgg
     3181 aaacctcctt gcggaagatg aatattcggg gactccttac tctactgcga tgaccaatgg
     3241 tgaacaagtt ggtcgtgttg gcggggatgt cttcttctca gggctgctca aaaaggctct
     3301 ctgacgaggt cgcagggagc gggacgactg ag
//
LOCUS       DATIPF010000046         3614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_712643, whole genome shotgun sequence.
ACCESSION   DATIPF010000046 DATIPF010000000
VERSION     DATIPF010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712643"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..768
                     /gene="glyS"
                     /locus_tag="VLL06_01220"
                     /pseudo
     CDS             7..768
                     /gene="glyS"
                     /locus_tag="VLL06_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014183.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
     gene            829..1224
                     /locus_tag="VLL06_01225"
     CDS             829..1224
                     /locus_tag="VLL06_01225"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HSS29616.1"
                     /translation="MKALESVTTKPEFDPLIVGFKRAHRLVEKEQWDRKPIDQARFEH
                     PTELALYQAVADEGKRLKLLDALNSGEYGQTLDALVGLKPAIDAFFTAVMVNADDPTV
                     RSNRLSLLKQVDDLFMSFADFSQIVVQGG"
     gene            1309..1800
                     /locus_tag="VLL06_01230"
     CDS             1309..1800
                     /locus_tag="VLL06_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29617.1"
                     /translation="MDHSLSDDQRRAKKIRRFTIAVSLALIVLCNTIMLVGLWASGIN
                     LDELATPPDVFNSKQDICLRLGWQSVVGSAEPVQLCSEWINLSDKSGKTHQLQQGVKL
                     RHGPDGQYYVDRGTQADYRLLILGLIVVAVIALGLAAKWYLVCRYRLHLESTGGHGAS
                     AVH"
     gene            1835..>3614
                     /locus_tag="VLL06_01235"
     CDS             1835..>3614
                     /locus_tag="VLL06_01235"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HSS29618.1"
                     /translation="MAKKYVYYFGDGAAEGTSNMKELLGGKGAGLAEMTNLGISVPPG
                     FTISTEACVEYYKNGKQYPPGMWDAALKSLKRVERSMDMGFGNPERPLLVSVRSGARA
                     SMPGMMDTVLNVGLTTKTVEGLAAKTRNDRFAQDSYRRFVAMYGSIVMGVPREHFEAI
                     LKQKKAEVGVPHETHLDARHLRELVASFKTLINEETKKEFPDEPLEQLRLAVNAVFSS
                     WFGARAVTYRRLYGIPDSWGTAINVVAMVFGNMGETSGTGVAFTRDPASGDKNFFGEC
                     LMNAQGEDVVAGIRTPLPVNALAKNVPAAYKELEHTYKRLEKHYRDMLDLEFTIQEGK
                     LYMLQTRVGKRTGIAAVKIAVDMVREGLISKKEAVQRIGPEQLAQYLYPIFDSNEEAK
                     SNPLGKGLPAGPGAAAGKIALTPDRAVTMKEAGTRVVLVRQETSPDDIHGMNAAEGFL
                     TARGGMTSHAAVVARQMGKVCVAGCEAVEVLDSQSVRIGAKVFREGDYLSINGSTGNV
                     YEGDLPVVESEIIQVIQGKLDAKKSDKYQRFALILSWADKVRKLRVRANADVPDQANI
                     ARGFGAEGIGLCRTEHMFFAEDRIPIM"
BASE COUNT          821 a          873 c         1154 g          766 t
ORIGIN      
        1 attgcggtgg caaacaatcg agccaaagac atgggattga tccgtgaagg aaatgagcga
       61 gtcttagcgg ctcggctggc agatgcgaag ttctttttcg acgaagaccg aaaggtcaag
      121 ctcgaggaac gtataacgaa acttgccggt gtcacattcc atcagaagct tgggaccatg
      181 cagaaaaagc agcaacgggt caggaagctg gcctccttca tcgcatcgag cgtgggacct
      241 gatgacggtg aattaaaaca ggcgtgcgag agggcggcgg atttgtctaa ggctgacctt
      301 ctcactggcg tggtcggtga gttcccggaa ctgcagggca tcatgggggg ggagtacgcg
      361 aaacacgatg gagaatctaa ggcagtcagt caggccatca gggagcagta tctgcctcga
      421 tcgatcgaag gtgacttgcc acaaaccata ggcggtcaag tgctgtcgtt ggccgaccgg
      481 ctggattcgt aggcggcgtt cttccatgtg ggtctcgtac cgaccgggtc agaagatcct
      541 tttgcgttgc gcaggaacgc cacggccatc gtcaggatta ttcttgaacg aaacctacgc
      601 atgaacttgg ggctctacat cgacaaagcg agaaacttgg tcatcgagga tggcttcaag
      661 ggggtcccag actctgagca ggagggaagg cgccggatca cggaattcat ttttgagcga
      721 gttcgccatt atagcagggt cgtccatgcg ttgcgagatg atgtcatcga agcggtgctc
      781 aaacaggctc atgacaaggc gttggacctc atggatcttg ttctgagaat gaaggcgcta
      841 gaatctgtca cgacaaagcc ggaattcgat ccgttgatcg tcggcttcaa acgcgcacac
      901 cggcttgttg aaaaagagca gtgggaccgt aagccgatcg accaggcgcg gttcgagcat
      961 cccaccgagt tggcgctcta ccaggccgtg gcggatgaag gaaagaggct gaaattgttg
     1021 gatgcactga actccgggga gtatggccag accctggacg cgctcgtggg cttgaagccc
     1081 gccatcgatg catttttcac agcagtcatg gtcaatgcag atgatccgac cgtccgaagt
     1141 aaccgattgt cgttgctcaa acaagtggat gatttgttta tgtcattcgc ggacttctcg
     1201 cagattgtgg tacaaggagg gtaaggtata ggtggtctgg atgagtcccc cgtgcgcgcg
     1261 gaatagggga cccaccgatc aagtgagaca gaacgatcag gtgtggggat ggatcactca
     1321 ttgtcagacg atcagcgtcg ggcgaagaaa atccgtcggt tcaccatcgc tgtgtctctt
     1381 gctctgatcg tgctctgcaa cacgatcatg ttggttggat tatgggccag tgggatcaat
     1441 ctcgatgagc tggcaacgcc ccctgatgta ttcaactcca aacaggatat ttgtctccga
     1501 ctcggctggc agtccgttgt gggatcggcg gaaccggttc agctgtgttc ggagtggatc
     1561 aatctttccg acaagtccgg taaaactcac cagctacaac agggtgtcaa gcttcggcat
     1621 ggaccggacg gacagtatta tgtggatcgg ggaacccagg ccgactatcg gttgctcata
     1681 ttaggactga ttgttgtggc agtcatagcg ttagggttgg cggcgaagtg gtatctggtc
     1741 tgccggtatc ggctgcatct cgaatccact ggcggccacg gagcatcggc cgtccattga
     1801 attgaactga cgtcaacgcg aaggagaggg cagcgtggca aagaaatacg tgtactactt
     1861 tggtgacggt gcggccgaag ggacctcgaa catgaaggag cttctcggcg ggaagggcgc
     1921 cggccttgcc gaaatgacga atcttggcat ctccgttccg cccggcttta ccatttcgac
     1981 cgaggcttgc gtcgaatatt acaagaatgg gaagcagtat cctcccggta tgtgggacgc
     2041 cgcgttgaaa tcgctcaagc gggttgagcg gtcgatggat atgggattcg gtaatcccga
     2101 gcggccgctg ctggtctcgg tgcggtccgg cgcgagggct tcaatgcctg gcatgatgga
     2161 cacggtgctg aacgtcggtt tgacgaccaa gacggtcgaa gggctggcgg caaaaacacg
     2221 caacgaccgc tttgcacagg acagctatcg gcgctttgtg gccatgtatg gcagcatcgt
     2281 tatgggtgtg ccacgggaac atttcgaagc gatcctcaag cagaagaagg ccgaggtagg
     2341 cgtgccgcac gagacccatc tcgatgcgcg gcatcttcgg gaattggtcg cgagctttaa
     2401 gacgctgatc aatgaagaga ccaagaaaga gttccctgat gagccgctcg agcagcttcg
     2461 cctggctgtc aacgccgtct tttcctcctg gttcggggcg cgggccgtca cctaccggcg
     2521 tctctatggg attcccgact cgtggggcac ggcgatcaat gtagtggcga tggtgtttgg
     2581 gaacatgggg gagacgagtg gaactggtgt ggcgttcacg cgcgatccgg cttccggcga
     2641 taagaacttt ttcggtgagt gcctgatgaa cgcccaaggc gaagatgtcg tcgccggcat
     2701 caggactccg cttccggtga atgcgctcgc gaagaatgtc ccagccgcct ataaggaatt
     2761 ggagcatacc tacaagaggc tcgaaaaaca ttatcgggat atgctcgatt tggaatttac
     2821 gatccaagag ggcaagctct atatgctgca gacccgggtc gggaagcgga ccggcatcgc
     2881 tgcagtgaag attgcggtcg acatggtcag agaaggtctg atctccaaga aggaagcggt
     2941 gcaacggatc gggccagaac aattggcaca atatctctac ccgattttcg acagcaacga
     3001 agaggcgaaa tccaatcctc tgggaaaagg gcttcccgct ggtcccggtg ccgcagcagg
     3061 gaagatcgcc ctcactccgg atcgggctgt gacgatgaaa gaggctggta ctcgtgtggt
     3121 gctggtcaga caggaaacga gcccggacga tattcatggg atgaatgccg cggaaggatt
     3181 tctgacggcg cggggtggga tgacgtccca tgcggcagtc gtcgcccggc aaatgggcaa
     3241 ggtctgcgtg gcgggttgcg aggcggtgga ggtgctggat agccagtccg tcaggatcgg
     3301 agccaaagtc tttcgcgagg gggattatct gtcgatcaac ggctcgaccg gcaatgtata
     3361 tgagggggat cttcccgtcg tcgaatctga aatcattcaa gtgatccagg gtaagttgga
     3421 tgcgaagaaa tcggacaagt accagcgctt cgcattgatt ctgtcatggg ccgacaaagt
     3481 gcggaagctc cgtgtccgtg ccaatgcgga tgtgccggat caggcgaata tcgcgcgtgg
     3541 cttcggggcg gaagggattg gcctctgtcg gacggaacat atgttctttg ccgaggatcg
     3601 tattccgatc atgc
//
LOCUS       DATIPF010000047         3459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_625522, whole genome shotgun sequence.
ACCESSION   DATIPF010000047 DATIPF010000000
VERSION     DATIPF010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625522"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(504..683)
                     /locus_tag="VLL06_01240"
     CDS             complement(504..683)
                     /locus_tag="VLL06_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29619.1"
                     /translation="MLGEHRAITITMRYAHLAPSHLRAGIQVLEQRNTRQTGGPELST
                     ELRVTPVSQDIAGIA"
     gene            1104..2477
                     /locus_tag="VLL06_01245"
     CDS             1104..2477
                     /locus_tag="VLL06_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HSS29620.1"
                     /translation="METALLYDRLQFTITVTFHYLFPQLTMGLALLLLYFRTRALMTG
                     DIHYHEVAKFWTKIFALNFAFGVVTGIPLEFQFGTNWAKFSTFAGGVIGQTLAMEGIF
                     AFFLESSFLGILLFGDKRFTVLVQRMATLMLFLGAWLSGYFIIATNAWMQHPVAYSLA
                     QDGHVFVNSLSGLLTNPWIFWQYAHTMTAAVVTSSCVMAAVGALYMLMGLHVTHAKTF
                     MQTGVMAGAIACALMIFPTGDGNAKQVFTHQPLKGAAFEGLFRTERGAGLILVGQPNM
                     ETLTIDNPLVIPRMLGFLIYEELQAELKGLDAFPKEQWPDNIPLLYYAYHIMAGLGTI
                     LASVMSVSLLMLWRGWLFRSKWLLWLLLLSAPFPYIATTAGWMTTELGRQPWLVYGLL
                     RTAEGASPLVHSGNVLFTLLGFLGLYCLLGLVFLFFMGQTIREGPVPLVLKTDRLAVD
                     GKDGHGY"
     gene            2480..>3459
                     /gene="cydB"
                     /locus_tag="VLL06_01250"
     CDS             2480..>3459
                     /gene="cydB"
                     /locus_tag="VLL06_01250"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HSS29621.1"
                     /translation="METFWYTAVTLMLGLYVVLDGFDFGVGILYPFLARSEADREAMR
                     DAIGPVWNGNEVWLIGAAGLLFFAFPKAYASGFSGFYLALILVLWLLMFRGLAFELRS
                     HVEHPLWRQLCDFIFSAASLSLAFVFGAALGNLIRGVPLNREGYFFVALWTDFFTGPE
                     PGILDWFTVLMGLASVAILALHGANYLAMKTEGDLYARAVATANLMGRVALGSAVLVG
                     LGITIVQPNLLVNYTAHPVGNIFPIGGAIAIVAMLVLRRRHYDVAAFMTSCLLILALL
                     GSVAWGCYPNILIATTDQANSLTIFNATTGSYGLQAGFVWFVIGFVLIIV"
BASE COUNT          654 a          986 c          976 g          843 t
ORIGIN      
        1 tttgctggac attgagcata ttgcaaaccg ttaccggtca cgtctcaccg cttcacatga
       61 gccaaatcct cgcgcccgca gtcctccacc aacagatatc gaccaatgag atccggcaga
      121 ctcgtgcacg acttgaggcg atcgtgtccg gatacagggc cgcatgggag caaactttgc
      181 gtctgcaagg agatcaccaa ccttctcaag cggcgcgccg tactggaggg ccagactcat
      241 caggcgggca atcacgtcgt acaaaccgat taattctgac gagcagtcgg accctttgag
      301 gcgaagaaag atttctgggg ggtgttcgtc ctcatgaacg ctgatgtaga gcgtccgttt
      361 gccggcgact ttgactttct gggcgatgtg attgcggcgg gaggggagag caaggcgggt
      421 accaccatgc cttggaaggg gatttcccag gaaggaggcg cggcggtgca aattccctca
      481 aatcccgttg aggacaccag cacttatgcg attcccgcaa tatcttgtga caccggcgtg
      541 acacggagtt ctgtggataa ctcgggtccc cctgtctgac gcgtattccg ctgctccaac
      601 acctgaatcc ccgcccggag atggctgggc gccaggtgcg catagcgcat cgttatcgtg
      661 atcgcgcgat gctccccgag catgctgtcc cataacattc ggtttctagt ttagctctcc
      721 tctgatccag tgagtccgac gactcgccga ccgctgatga cccaatgggc taaaccacga
      781 cggggacaaa ccagccctcc cgttcacctc ctgtccagaa tcctagtcga aatccctcca
      841 gtggacatct cgtcttacca aatccttcgg cgtatgattt cctgtggacc aaaacaaatc
      901 aagttgatgc cgcaccatgc gatcggtcga aatttgagga ggccacgtct gagcccatta
      961 cgtgttcatc cggcgctgaa gatacaggat cagatccgag accgctgttc atgcctctca
     1021 cgcggcacac tgcagaatcc gaagcgccct ggcgtgcttg aatcactgcc gtagcaaaga
     1081 gggatcgcta cagaaaggct gcgatggaaa ccgccctgct ctacgaccgc ctccaattta
     1141 ccattactgt cacgttccac tacctctttc ctcagctgac catggggctg gccttgctgc
     1201 tcctctattt ccggacacgc gcgctcatga cgggcgatat ccattatcac gaggtggcga
     1261 agttctggac caagattttt gctctcaatt tcgcgttcgg cgtcgtcacc ggcatcccgt
     1321 tggaattcca gttcggcacg aattgggcga agttttctac ctttgcgggc ggtgtgatcg
     1381 gccaaacttt ggcgatggag ggcatctttg ccttcttcct cgaatcgtcg ttcctgggta
     1441 tcttgctctt cggcgacaag cgtttcaccg tattggttca aaggatggcg acattgatgc
     1501 tctttctcgg cgcatggctg tccggctatt tcatcatcgc gacgaatgcc tggatgcaac
     1561 atcctgtcgc ctattcactg gctcaagacg ggcacgtatt cgtgaacagc ttgagcggcc
     1621 ttctcacgaa tccgtggatc ttctggcaat acgcgcacac catgacggcc gccgtcgtaa
     1681 cgtcgtcttg cgtcatggcg gccgtgggcg ccctgtatat gctcatgggt ctccacgtga
     1741 cccatgccaa aacattcatg cagacaggcg tcatggccgg cgccatagcc tgtgcgctca
     1801 tgatctttcc gacaggggac ggcaacgcca aacaagtctt cacgcatcaa ccgcttaagg
     1861 gcgcggcctt tgaaggctta ttccggaccg agcgcggcgc cggattgatc ctcgtcgggc
     1921 aaccgaacat ggagacctta accatcgata atccgttggt gattccaaga atgttaggtt
     1981 ttctgatcta cgaggaactg caggctgaat tgaaagggct cgatgcattt ccgaaggaac
     2041 aatggccgga caatatcccg ctgctgtatt acgcgtatca catcatggcg ggtcttggga
     2101 cgatcctggc gtcggtgatg agtgtttcgc tcttgatgct gtggcgtggc tggttgttcc
     2161 ggtcaaaatg gctattgtgg ctgctcctcc tctctgcccc cttcccctat atcgccacga
     2221 cggccgggtg gatgacgaca gagctgggcc gccaaccgtg gctggtatat ggtctgctgc
     2281 gtaccgctga gggcgcatca ccactcgtgc actcgggcaa tgtcttgttc acattactgg
     2341 gattcctggg cctgtactgc ttgttgggac tggtgtttct tttcttcatg ggtcaaacca
     2401 tccgtgaggg gcccgtgcca ctggttctga agacagacag gttggctgtt gatggaaagg
     2461 acggccacgg atactgatca tggagacctt ttggtacacc gcagtgacgc tgatgctggg
     2521 cctctatgtg gtgctcgacg gctttgattt cggtgtagga atcctctatc cgttccttgc
     2581 gcgaagtgag gcggatcgtg aggcgatgcg cgacgcgatc ggcccggtgt ggaacgggaa
     2641 cgaagtctgg ctaatcggag cagctgggct actgttcttc gcgtttccca aggcctacgc
     2701 gtccgggttc agcggtttct acctcgcgct gatccttgtt ctatggctgc tcatgttccg
     2761 tggactagcg tttgaattgc ggtcgcatgt ggagcatccg ctgtggcggc agttgtgcga
     2821 cttcatcttc tcggccgcga gtctctcgct ggcctttgtc ttcggcgccg cgctaggcaa
     2881 cttgattcgc ggtgtaccgc tgaatcgcga gggctatttc ttcgtcgcgc tgtggacaga
     2941 tttcttcacg ggtccagagc cagggattct ggattggttc acggtactta tgggacttgc
     3001 cagtgtcgcg atcttggcct tgcatggcgc caactacctc gcgatgaaaa ccgagggcga
     3061 cctctatgcg cgggctgtgg ccacggcaaa tctgatgggc cgggtggcgc ttgggagtgc
     3121 cgtcttggtg gggctgggga ttacgatcgt acagccgaat cttctggtta actataccgc
     3181 ccatccggta ggcaatatct ttcccatcgg cggagcgatt gccattgtcg ccatgctcgt
     3241 gctccgacga cgacattatg acgtggccgc cttcatgacc tcgtgcctgc tcatccttgc
     3301 cctgctcgga agtgtcgcct gggggtgcta tcccaacatc ctgatcgcga ccacggacca
     3361 ggccaacagt ctcaccatct tcaatgccac taccggttcc tacggcctgc aagccgggtt
     3421 cgtgtggttt gtcatcggat ttgtgctgat catcgtcta
//
LOCUS       DATIPF010000048         3988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1333638, whole genome shotgun sequence.
ACCESSION   DATIPF010000048 DATIPF010000000
VERSION     DATIPF010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333638"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="VLL06_01255"
     CDS             complement(<1..1019)
                     /locus_tag="VLL06_01255"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HSS29622.1"
                     /translation="MQTNNHVAGFWVKGWWTIRKVGGYRQLRAWNLVVAAISCFLLTA
                     SGCTKQDPYSPPDLFYYFASYKVGKNPTTVTPTDVNQDGFTDLVTTNMGSNTLSILLG
                     NGDGTFHDQVELNVCKEPRSLALGMFNRDPYPDVVLACSGADEVAILFGRADGKFQEG
                     TRYPVHRTPIAIAIDDLNGDQALDLVVALRNDKIKVFLGTGTGEFTHGAQYEYGDTPT
                     SVALADLDGDGKIDLAVTNGGPMLNAVSIWMGNGDGTFRPPTDYRTGKRPLGVSFADF
                     NHDRIRDLLVINGVQDSFTTFLGNGNGTFQPGRDSGADAGPNFGLARDFNGDHRTDVA
                     IVNLQS"
     gene            1218..1538
                     /locus_tag="VLL06_01260"
     CDS             1218..1538
                     /locus_tag="VLL06_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29623.1"
                     /translation="MSKILKIELSMYGVAEVLHWCHDRNKGRVPGVDTAGFEKMKVLL
                     TEKPQSGDYFILDQFWKKRVTLELTEEEVATIDRCLYDIPNFDNEPLPQIRHKFWPQE
                     AAAH"
     gene            complement(1824..2825)
                     /locus_tag="VLL06_01265"
     CDS             complement(1824..2825)
                     /locus_tag="VLL06_01265"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HSS29624.1"
                     /translation="MNAPDSHDAVGIDVRWTEEASTRLERAPLFLRGMVRRLAEKKAR
                     ELGYAEITEEILDQFKHQMMGRMGGESSMADAAAQMEQGHLPWTAAAKDRLNTVPEFM
                     RHMIRQIAEEIASERGHLEVNVELFEKVEALGDLRESSVPPLEWSEGALAQLQEKLKQ
                     SPQIAVEFVTDMLKRDTEDLAREQGLTRIDESVLLQLWDAPKGRVGWTDEAWKRLQTA
                     PDFVRSGIRKAAERRARKLGLKEIDSEHLTTFRNQAMMKAVKRIRSFGYNELTFEAFD
                     TALEKTKRLQGNEQAEKRLQEIRGHFADPDVKKPEGGTLGADLMGRFRSYLKGEGTL"
     gene            complement(2839..3120)
                     /locus_tag="VLL06_01270"
     CDS             complement(2839..3120)
                     /locus_tag="VLL06_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HSS29625.1"
                     /translation="MTKAQIIERVSEQVTTLTKRQAEVVVNTIFDCVRDSLKNGDKTE
                     IRGFGSFRLRARRMKEGRNPKTGATVAVPAKKVPFFKAGKELKELLNQS"
     gene            complement(3174..>3988)
                     /gene="sppA"
                     /locus_tag="VLL06_01275"
     CDS             complement(3174..>3988)
                     /gene="sppA"
                     /locus_tag="VLL06_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HSS29626.1"
                     /translation="LFLVNLFFPDLDISMEDRVGLIRIEGVILDSQSTIGELKRFGEN
                     PAIKAIVLRIDSPGGGVVPSQEIHDAVQRVRNKNNKAVIASMGSVAASGGYYIAVATD
                     RIMANSGTLTGSIGVIMEMANVEGLLKKIGVEGVVIKSGRFKDVGSPLRKMSDEEHEL
                     LQSVMDDVHKQFIEAVAAGRGLDVTVVQALADGRIFTGRQAKDSKLVDELGDLEAAIQ
                     LAADVAGIEGVPKVIEPRRRFSFRELIDSRLSTLFPKLDLYSGVSLKYLMAF"
BASE COUNT          892 a         1177 c          991 g          928 t
ORIGIN      
        1 gattgaagat tgacaatcgc cacatcagtg cgatggtcac cgttgaagtc gcgagcgagt
       61 ccaaaattcg gtcctgcatc ggcaccggaa tcacgtcctg gctggaacgt cccattgccg
      121 ttaccgagaa aggttgtgaa actatcctgc acaccattga tcacaaggag atcacggatc
      181 cggtcatgat tgaaatcggc gaaactcacc ccgagcggtc gctttccagt ccgataatcg
      241 gttggagggc gaaacgtgcc gtcgccattc cccatccaga tggagactgc gttcaacatc
      301 ggccccccgt tggttacagc caaatcgatc ttcccatcgc catccaaatc ggctaatgcc
      361 accgatgtgg gcgtatcgcc gtactcatac tgcgcgccat gggtgaattc gccggtgccg
      421 gttcccagaa aaacttttat tttgtcgttg cgcaacgcga ccacgagatc gagcgcctga
      481 tcaccgttca gatcgtcgat ggcgatcgca atgggagttc gatgcacggg atagcgcgtc
      541 ccttcctgaa attttccgtc ggctcgaccg aagagtatgg ccacttcatc ggccccggaa
      601 caggccaaga ccacatcggg atacggatca cggttgaaca tcccgagagc gagcgagcga
      661 ggctctttac agacattcag ttcgacttga tcgtgaaagg tgccgtcccc gttgcctaag
      721 agaatcgaaa gggtattgct gcccatgtta gtggtcacca aatcggtaaa accgtcctga
      781 ttgacgtcag taggggtcac ggttgtcggg ttcttaccaa ctttgtagct agcaaagtaa
      841 tagaagagat ctggtgggga gtaagggtct tgcttggtac agccgcttgc cgtaagcaga
      901 aaacaactta tcgccgcaac cacaagattc cacgctctaa gttgcctata gccgcctacc
      961 tttctgatcg tccaccaccc tttgacccag aaaccagcaa cgtgattatt cgtctgcatg
     1021 aacacgtgcc tcttaaccag gccttcccac ctaaacgagt gtgccagtat acagaccagc
     1081 gctttctcct ggcaatcagg ccaaccgcgc aacgatcctc gttaacacgc ggtttcctca
     1141 atcagggtct ccacaacgag cacttttaaa cgggttcgtg tttggggtac aatgccagcc
     1201 ttggtaagga ggaatccatg agcaagatac tgaagattga gttgtccatg tacggagtcg
     1261 ccgaggtgct gcattggtgt cacgaccgca acaagggccg cgtccccgga gtggacacag
     1321 caggcttcga aaagatgaag gtgctcttga ctgagaagcc tcagtcaggc gattatttca
     1381 tccttgatca gttttggaag aagagagtaa ccttggagtt gacagaagaa gaggtggcga
     1441 cgatcgatcg ctgtctctat gacatcccaa atttcgataa cgagcctctt ccacagatcc
     1501 gccataaatt ttggccgcag gaagctgctg cccactaagc agcaggggcc gtcctcaaac
     1561 ggctcctgcc tctacctcct agcaattcgc ccagcctact agtccaagac ggggccgaga
     1621 gctgcgcgga gaacctgtag cgttttgatt cgagcttgcg ggaagaacca cctggctgga
     1681 aaaagaaact gcccttgcgg gatgctcaaa aagtctgtcc agcaaggccg cagtgagtga
     1741 aagcccgagg cgtacccttt gggtacgttg agggcttgaa cgaagtgaga acgaagctgg
     1801 cggccttttt cagcatcctg ccgctagagc gtcccttctc ccttaagata gctgcgaaag
     1861 cggcccatta gatcggcacc aagtgtccca ccttccggtt tcttcacatc cggatcggca
     1921 aaatgcccgc gaatttcctg aaggcgtttt tccgcctgct cattcccctg caggcgcttc
     1981 gtcttctcta gcgctgtatc gaatgcctca aacgtcagct cgttgtaccc gaaagagcga
     2041 atccttttga cagctttcat cattgcctga ttccggaaag tcgtgagatg ttctgaatca
     2101 atctctttga gcccaagttt ccgcgccctc cgttcggcgg ctttccggat tccactacgc
     2161 acaaaatcgg gagcagtctg caatcgcttc catgcctcat cggtccatcc gactcggccc
     2221 tttggtgcgt cccacagctg aagcaacacc gattcgtcga tacgtgtcag cccttgctct
     2281 ctcgccaagt cttcggtgtc acgcttcaac atgtcggtca cgaattcaac ggcgatctgt
     2341 ggggactgtt tcaacttctc ttgaagctga gccaacgccc cctcgctcca ctccaacgga
     2401 gggaccgagg actcccggag atcccccagc gcttcaactt tttcgaagag ttcgacattc
     2461 acctcaagat ggccgcgctc actggcgatc tcttcggcaa tttgcctgat catatgacgc
     2521 ataaattctg gaacggtatt cagccggtcc ttggcagcgg cagtccatgg gagatggccc
     2581 tgctccatct gtgccgcagc gtccgccatg ctggattcgc cgcccatacg gcccatcatt
     2641 tggtgcttga actgatcgag aatctcctct gtaatctccg cgtaaccaag ttctcgagcc
     2701 tttttttcgg ccagacgacg gaccattcca cgcaagaata gcggtgcccg ctctagacgc
     2761 gtggacgcct cttccgtcca acggacatcg atgccaactg catcgtggga gtctggtgcg
     2821 ttcatgatga gcatcctgtt atgactggtt caggagttcc ttcaattcct tgccggcttt
     2881 gaaaaatggg accttcttgg cagggaccgc cacggtggcg ccggtctttg gattccgtcc
     2941 ttctttcatc cgacgagccc gcagccgaaa gctgccaaat cctcgaatct ctgttttgtc
     3001 gccgttcttc aacgaatcac gcacgcaatc gaaaatcgta ttgacgacga cttccgcctg
     3061 ccgcttcgtc aaggttgtga cctgctctga tacgcgttcg atgatttggg ccttcgtcat
     3121 gttggactcc cctttcgtgg tgatgaatcg acctgcccgt cgatccgacc gcgctagaac
     3181 gccatgagat acttgagact gactccggaa tacaagtcca attttggaaa gagcgtcgac
     3241 aatcgcgaat cgatcaactc tctgaacgaa aatcgtcggc gcggctcaat gactttcggc
     3301 acgccctcga tgccggccac atccgcggca agctggattg ccgcctcgag atcaccgagt
     3361 tcatccacaa gttttgaatc tttggcctga cggcccgtga agattcgacc gtcagccaag
     3421 gcctgcacaa ccgtcacgtc gagtccccgt cctgccgcca ccgcctctat gaactgctta
     3481 tggacatcat ccatgacgga ttggagcaac tcgtgttctt catcactcat cttccggagc
     3541 ggcgatccta catccttgaa gcgaccgctt ttaatcacga caccttcgac gccgatcttc
     3601 ttgagcaatc cttcgacatt ggccatttcc atgatgaccc caatgctccc cgtcaatgtt
     3661 ccagaattgg ccatgatccg atcggtcgcg accgcaatat agtacccccc tgacgccgct
     3721 actgaaccca ttgaggcaat caccgcctta ttgttcttgt tacgaacccg ctggaccgca
     3781 tcatggattt cttgcgatgg cacaaccccg ccgccggggc tatcgattcg caagacaatc
     3841 gctttaatgg cggggttctc gccaaaccgc ttcaactcgc cgatcgtgga ttgcgaatcc
     3901 agaatgacac cttcgatgcg aatcagtcca acccggtctt ccatcgagat gtcgaggtca
     3961 gggaaaaaca ggttcaccag aaagagca
//
LOCUS       DATIPF010000049         3013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_272699, whole genome shotgun sequence.
ACCESSION   DATIPF010000049 DATIPF010000000
VERSION     DATIPF010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272699"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1016)
                     /locus_tag="VLL06_01280"
     CDS             complement(186..1016)
                     /locus_tag="VLL06_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HSS29627.1"
                     /translation="MLYHADCADGFGAAWAIWNQFPAARFIPVKHGNPPPIGLQDQRV
                     VIVDFSYARETLEPMAAQTQALLVLDHHITAEKALAGLPYAYFDMKKSGAVLAWEWAH
                     DQPIPWLLNYIQDKDLWAWALPSSREINAAVASYPFDYHLWSQFKQRELEQEGRAILR
                     YENELVNKLAAEAISVDFHGAVVPSVHSAVLTSQIGERLSAEHPFCVMWHDRDGRRYY
                     SMRSREDGTDVGAIAASFGGGGHTHAAGFSVSLGPDGSLPDNPALPRSVIDRRRLPSE
                     "
     gene            complement(1122..2165)
                     /locus_tag="VLL06_01285"
     CDS             complement(1122..2165)
                     /locus_tag="VLL06_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HSS29628.1"
                     /translation="MKTSQTRARPLPSNEELLNQIRILLDSPEDDLQAAIMKELLAGT
                     LRLHDSHLDILDLKIVNRAVKELRHAFRVFHDYRDRRKISIFGSARTASDDPNYELAF
                     QFSRKIVEEGFMVITGGADGIMRACQEGAGRENSFGVNIMLPFEQGPNATIADDPKLV
                     TFKYFFTRKLIFQKEANAIALFPGGFGTHDEGFEILTLAQTGKSDPQPIVCLQAPGCD
                     YWDEWYAFITHQLLARHLINPEDLSLFKITSSIDDAIDEIRNFYRRFHSIRYVGRLLV
                     MRLKTSITLEQVAGLHQAFGDLLSEGTFELRGALPEELDEPDLRDLPRLAFISNRQSA
                     SRLRQLIDHLNRF"
     gene            complement(2254..>3013)
                     /locus_tag="VLL06_01290"
     CDS             complement(2254..>3013)
                     /locus_tag="VLL06_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSS29629.1"
                     /translation="TPFKTTHLTRAMVAHMMTPGIVQIPGDVSVSEAALLLEREQAPC
                     LLIKDTDTRFGLMTPTDIVKKVVAQGLEPHDIEVRTIMTRPVQFIEYDEALADASTLM
                     VATGASLLIVTKQNQPVGVLSAQDLMLTPSRRDTKIPVVVSVLGGASAQGAHYNAMII
                     QLNHVGALVESATKLAPKSNLVLAFSISGQNTPLTIRGRVLASKIPEATPHEPTSETS
                     TWYVDIQFTDLSSADLSRIRAWALQTMPSSSTRF"
BASE COUNT          681 a          764 c          912 g          656 t
ORIGIN      
        1 ggaggccagc ctgtccgaac acgagggccg ggattgcgcc atattgaaag acgaatctct
       61 cgcctgagtt ggcgggaaga ctggcctcgt aaaaaaagac aaggacacag gtggcaataa
      121 aaccgatcgt gaccaccggc gtcaatttgg tcgggttatc gtcgtgcaga gggatcatgg
      181 ggccctcact ctgacgggag acggcgacgg tcgatgactg atcgagggag ggccgggtta
      241 tccggcaatg agccatcagg tccgagagag actgaaaacc ctgcggcgtg tgtatggcct
      301 ccaccgccga aggatgcagc aatggccccc acgtcagtcc catcctctcg cgagcgcata
      361 ctgtaatagc ggcgtccatc gcgatcgtgc cacatgacgc aaaacggatg ctctgcagag
      421 aggcgttcac cgatctgact tgtcaagacc gcactgtgga ccgagggaac gacggctccg
      481 tgaaagtcga cggagattgc ttccgccgca agtttgttca cgagctcgtt ttcataacgc
      541 aggatggctc ggccttcctg ctccaactcc ctctgtttaa actgactcca gaggtgataa
      601 tcgaatgggt aggaggcaac ggcggcattg atttcccgac tggaagggag cgcccaagcc
      661 cagaggtctt tgtcttgaat atagttgaga agccatggta tcggctgatc gtgtgcccat
      721 tcccaggcca ggaccgcacc agacttcttc atgtcaaaat acgcataggg aaggccggca
      781 agggctttct ccgcggtgat gtgatgatcg agcacaagga gcgcctgcgt ctgagcagcc
      841 atgggttcca gcgtctctcg ggcgtagctg aagtccacaa tgacgactcg ttggtcctgt
      901 agccctatgg gaggtggatt gccgtgtttg acgggaatga accgagccgc tgggaattga
      961 ttccagatcg cccaggcggc gccgaatcca tcagcgcaat cggcgtgata tagcacgatg
     1021 gtgggaggat cagggaacgg gtgctgtgtg cggaagccag ccattgtggc agagcatacc
     1081 atgatgcccc gccgagacta aagcgtcttt gtgaggagag gtcagaatct attgagatgg
     1141 tcgatcagct gacgcagtcg acttgcgctc tggcggttgg agataaaggc gaggcgaggc
     1201 aagtccctca gatccggctc gtcgagttct tcaggtaggg caccgcgaag ttcaaaagta
     1261 ccctcggata acaggtcccc aaatgcctga tgaaggccag ctacctgctc gagcgtgatg
     1321 ctcgttttga gcctcatcac caagagccgt cccacatatc gaatggagtg gaatcggcgg
     1381 tagaaattcc tgatctcatc gatcgcatca tctatagacg acgtgatctt gaataggctg
     1441 agatcttcgg ggttgatcag atgacgggcc aaaagttggt gtgtgatgaa ggcgtaccac
     1501 tcgtcccaat agtcgcaacc gggagcctgg agacagacga tcggctgggg atcgctcttg
     1561 cctgtttgag cgagggttaa aatttcgaaa ccttcatcat gtgtcccgaa tccgccaggg
     1621 aacaatgcaa tggcgtttgc ctctttctga aagatgagtt tgcgggtaaa gaaatatttg
     1681 aacgtgacga gcttcgggtc atcggcaata gtggcattcg ggccttgttc gaaaggcagc
     1741 ataatgttga ccccgaagct gttttctcgt ccggcgcctt cctggcacgc acgcatgatt
     1801 ccatcggccc cccccgttat aaccatgaat ccttcctcga cgatcttacg ggaaaactgg
     1861 aaagccagct cgtagttcgg atcatccgag gcggtgcggg ccgaaccgaa tatgctgatc
     1921 ttgcgccgat cgcgatagtc atgaaagacg cggaaggcgt gacgtagttc cttgacggcg
     1981 cggttgacga tcttgaggtc cagtatgtcc aaatgagaat cgtggagccg gagcgtacca
     2041 gccaggagtt ccttcatgat ggccgcttgc aggtcgtctt ccggactatc cagcaggata
     2101 cgaatctggt tgagcagttc ttcgttcgaa gggaggggac gggcacgggt ctgcgaggtc
     2161 ttcatcgtat tatccatgtg cacggtccga ggaacacaaa aattctatca gctgattacc
     2221 tattggtcaa aagaacgaag catatgtccg tgattagaac cgagtggatg aagaaggcat
     2281 ggtttgaagt gcccaggccc tgatacgcga gagatcggca gacgaaaggt cagtgaactg
     2341 aatatcgaca taccaggtac tggtctcgct tgtcggctca tggggcgttg cttcgggaat
     2401 cttgcttgcc agaacccttc cacgaatggt caggggagtg ttttgaccag aaatggaaaa
     2461 cgccagtacg aggttcgatt ttggcgcgag cttcgtcgcc gattcgacca acgcaccgac
     2521 gtggttgagc tggatgatca tggcattata atgagcaccc tgtgccgagg ctcctccgag
     2581 cacactcacg acgacgggga tcttcgtatc acgtcgggag ggcgtgagca tcaggtcctg
     2641 tgcactgagt accccaaccg gctggttttg cttggtgaca atcaagagag aggcacctgt
     2701 ggccaccatc agtgtcgagg catcagccaa ggcctcatcg tactcgatga actgtacagg
     2761 gcgtgtcatg atcgtccgga cttcgatgtc gtgaggttcg agcccctgag cgacgacttt
     2821 cttgacgatg tcggtcggcg tcatgaggcc gaatcgggtg tcggtatcct tgatgagcag
     2881 gcagggcgcc tgttctcgtt cgagcagcag cgctgcctcg ctgaccgaga cgtctcccgg
     2941 aatttgcacg atccctggtg tcatcatatg ggcaaccata gcgcgtgtga gatgtgttgt
     3001 cttgaagggc gtg
//
LOCUS       DATIPF010000050        12428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_361158, whole genome shotgun sequence.
ACCESSION   DATIPF010000050 DATIPF010000000
VERSION     DATIPF010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 12428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12428
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361158"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /locus_tag="VLL06_01295"
     CDS             complement(<1..1402)
                     /locus_tag="VLL06_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HSS29630.1"
                     /translation="MKSQGHVSTGNTHDVIVIGGGSAGYAAARTARDAGADVAIVDHG
                     PLGGLCILRGCMPSKAILRSAEVAALLRRAKEFGLSPVSTRADLSAIVDRKDRLVREF
                     ADYRIQQLRDPSLTLYQSQAVFRSPHEIVVGDTILSSGSFILATGSRPDEVPIPGLAQ
                     AGYFTSDTILDLRTLPDSLIVLGGGSVALELGQFFARLGTRVTILQRGRTLLSEMDED
                     VGRALEAALIDEGIEVVTGVQLLRVICDERGKTVWAMKDGRERSYAGEEILQALGRRP
                     NIEGLRLDLAEVIVDSGRIVVDGAMRTSQPHIYAVGDVNDVTPIVHLAIQQGEIAAYN
                     VTHPDRPAKELDHRLDAEVVFTDPQVAVVGLNDAACHAGKIPHLTASYPFADHGKALC
                     LGASHGFVKLLCEPTTGELLGAQIVGPEAGELIHELIAVMYYHGTASDLLRMPHYHPT
                     LAEIVTYPAESIVEQLG"
     gene            1448..1660
                     /locus_tag="VLL06_01300"
     CDS             1448..1660
                     /locus_tag="VLL06_01300"
                     /inference="COORDINATES: protein motif:HMM:NF019481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HSS29631.1"
                     /translation="MVQPGRYRHYKGHEYEVLGVARHSETEEEYVVYRALYGDGGLWV
                     RPTDMFLETVLIDGRPCPRFRFLTPP"
     gene            1793..2416
                     /locus_tag="VLL06_01305"
     CDS             1793..2416
                     /locus_tag="VLL06_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSS29632.1"
                     /translation="MPIIRAVNGIIETYPSDPVSPRPPATRVPSAGREDHSKSQERQT
                     ADHAARLAQRAYQQQAHQATEPKPALLAQDLMTSPVTWLPSSSTLLEAWTVMTRKGIH
                     HLPVTSIHGTLVGMISNLELLPYARELESVDSPGPSAEHKLARVMNSRVLSATPTTEI
                     REIAHIMLDEHVNAIPILDSSRHPVGILTTSDILRAIVHRSPLELWT"
     gene            complement(2484..2954)
                     /locus_tag="VLL06_01310"
     CDS             complement(2484..2954)
                     /locus_tag="VLL06_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HSS29633.1"
                     /translation="MVPQVWTDPDVIEWSQLLLNSFRHWTGRELLERVGDPAYQAHAL
                     FLSPFVVVSHGMEENPLINYGNQMALELWEMTWEDLTRTPSRLTAEPTNRAEREWMLE
                     QAKTRGYLDTYQGVRVTSTGRRFLVENALIWNVLDAERQRIGQAATFMHWTWLP"
     gene            3140..4543
                     /gene="leuC"
                     /locus_tag="VLL06_01315"
     CDS             3140..4543
                     /gene="leuC"
                     /locus_tag="VLL06_01315"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HSS29634.1"
                     /translation="MAAQTLFDKIWESHVVRAEPDGTTLLYIDRQLVHEVTSPQAFEG
                     LKLSGRRPRRPGATLAVPDHNVPTTDRTVGITDPLSAKQIQTLEENCHDFSIALFGMN
                     DIRQGVVHVIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLV
                     QKRPKTMEVRVEGMLSSLCSAKDIILAIIGKIGTAGGTGYVIEYTGSAIRALSMEGRM
                     TLCNMSIEGGARAGMVAPDEHTIAYIKGRPLAPKGELFEQAVHAWRQLKTDANATYDR
                     TVTMQAEDMAPQVSWGTSPGMVTGINQRVPDPRTITDENQRQATMRALEYMGLIPNMP
                     ITDIKIDTVFIGSCTNSRIEDLRLAANLTKGKHVARGVRAMVVPGSGLVKQQAEQEGL
                     DRLFKEAGFEWREAGCSMCLAMNADVLQPGERCASTSNRNFEGRQGAGGRTHLVSPAM
                     AVAAAVEGHFVDIRSWK"
     gene            4551..5174
                     /gene="leuD"
                     /locus_tag="VLL06_01320"
     CDS             4551..5174
                     /gene="leuD"
                     /locus_tag="VLL06_01320"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HSS29635.1"
                     /translation="MQPFTTMTGLVAPLDRVNVDTDQIIPKQFLKTIKRTGLREGLFY
                     DWRKQKDGSQDPTFFLNQPRFQGATILITRDNFGCGSSREHAPWALLDQGFRCIIASS
                     FADIFYNNCFQNGMLPVVLKADEVLAMMDDVLATPAYQLTIDLGNQTVTTPDNTHYRF
                     EIDPFRKDCLYRGLDAIGLTLQYEAAIAAYETRRKAEAPWLFTDLRL"
     gene            5190..5540
                     /locus_tag="VLL06_01325"
     CDS             5190..5540
                     /locus_tag="VLL06_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29636.1"
                     /translation="MKKLFLVLVVVCFIGASYLLIAFNYSYSDGSRAGYIQKFSHKGW
                     ICKTNEGELAMTTVPGVAPVLWAFSIWDNKVASQLSQAMGKRLVLHYKEYRYLPTTCF
                     GETAYFVDRVEITE"
     gene            complement(5669..6232)
                     /locus_tag="VLL06_01330"
     CDS             complement(5669..6232)
                     /locus_tag="VLL06_01330"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HSS29637.1"
                     /translation="MTRRRFLTVTIGALAFGGLFGAADAPAAPTDPSGITQNSGKVLP
                     AAQRFVILPAFNSEAVLDKETGLVWEKSPQTTMARWSAARRICTEKNVGGQQGWRLPS
                     LPELRSLVDSSVAPPGPTLPPGHPFLGVQSAVYWSETRVGDNPSGAWAVHFGLGGGSV
                     FINWAHGVQVWCVHGGVNADQHEGLGH"
     gene            complement(6399..7454)
                     /gene="corA"
                     /locus_tag="VLL06_01335"
     CDS             complement(6399..7454)
                     /gene="corA"
                     /locus_tag="VLL06_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HSS29638.1"
                     /translation="MKLVQKRSRKTGLPPGTLVHIGEKKTDKVTITAFNYAGTRCDER
                     RDLSLAELAPPTDGSVAWVDVGGIHNMDILESFGKQFQLHPLLLEDIANTDQRPKLDD
                     YDTYLFLVMKMLSVTDRQDIVVEQVSLVLGRNFVLSFQENGADVFTPVRDRLRGGKGR
                     LRQSGADYLLYALIDAIVDQYFTVVEVLGEKIEALQDLVVSDPKPETLHQIHALKRQL
                     LFLRRAVWPLREATNSLSRSECPLLQESTKVFFRDVYDHVVQIVDTIETLREMVSASL
                     DIYLSSISYRLNAVMKVLTIITTIFMPLTFIVGIYGMNFEHIPELKWEWGYPLVLGMM
                     AAIAIAMLGFFIRKKWI"
     gene            7607..8410
                     /locus_tag="VLL06_01340"
     CDS             7607..8410
                     /locus_tag="VLL06_01340"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HSS29639.1"
                     /translation="MDRELSTLVEAIRKAAVKVGEMVRDGFEIQTKPDHSPVTTVDLE
                     VNRILHEMQGREFPRDGWLSEESPDDPARLTHARVWIVDPIDGTKALVNGVPEFCISA
                     ALVERGAPVIAAILNPSTDELFTAVRGGGLFLNGTPLTHSLPHDFNPVIMVNAWELRI
                     GRWSTLAETSRCRPMYSIANALALVAAGRVQAALTIEPENEWDLAAGVLLIEEGGGTI
                     SDAAGKPFVFNQPTPMFHGVIAVAATADKGLRAKLQAHAEHALSHPPRT"
     gene            8419..9282
                     /locus_tag="VLL06_01345"
     CDS             8419..9282
                     /locus_tag="VLL06_01345"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSS29640.1"
                     /translation="MTIALLGTGLLGRAIAERLQSVGHTVTAYNRTTSKALPLQACGI
                     TVVTRPEEAIAQADCVVLMLADAAAIQAVLLTPASLVVLRGKTVIQMGTIAQDESLAL
                     QHEIEQVGGSYCEAPVLGSLAEAQAGTLLMMVGGTEGQFVQWGPLFRALCREPRLIGP
                     VGKAASLKLALNQLIVAEISAFALSLGLVQRAGVPVDTFMAILRESALFAPAFEKKLP
                     RLLTRDYQHPNFSTRHLLKDAELFLREASGYALTTSGLEGIRPLLERTIEQGLGDSDY
                     SAIFEIINPDQ"
     gene            9443..11215
                     /locus_tag="VLL06_01350"
     CDS             9443..11215
                     /locus_tag="VLL06_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HSS29641.1"
                     /translation="MTYAIGTMQKSAQKTIEPRAYDPLIHEGFIRQADLVKAVQESLQ
                     GVTDLEGLLIEKYRIPKAALGKTLSAFFDCPYIPYDKRTLADPQLLNNLSHDYLRRHH
                     WLPLKRQGDILDVLIDNPHDLDRGHDIQRAFPGLTIRYALGLRCDIERFLSATIGETD
                     SGSIADTLGELVNDAHHEQALDNDSDGIDENDSAIVRLANQIIVEAYRQEASDVHIEP
                     YSDRKETAIRFRVDGTCSTYMRIPAIYRRALVSRIKIMSSLDISERRKPQDGKIRFKL
                     NQDREIELRVATLPTAGNNEDIVMRLLTAKEPLPLDALEFAPATLTTLLEITEKPHGI
                     ILCVGPTGSGKTTTLHALLHHLNTDERKIWTAEDPIEITQEGLRQVQVHPRIGFTFAS
                     AMRSFLRADPDVIMIGEMRDKETADVAIEASLTGHLVLSTLHTNGAVETVIRLLDLGC
                     DSFNFADAMQGVLAQRLCKRICVNCKEPYHPTAQEFDKLVHGYGKHAWNVLGVGYRED
                     FQLYRGRGCDTCKHTGLKGRIALHELLRGSDELRNLIQTRAKTMEMQSLAVKEGMVTL
                     VQDGILKVLAGLTTYDQVRAVAMK"
     gene            complement(11246..11560)
                     /locus_tag="VLL06_01355"
     CDS             complement(11246..11560)
                     /locus_tag="VLL06_01355"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS29642.1"
                     /translation="MEGRKHTRFAVQLPASFGEHQPSHGGTILNVSAEGCAITSEAVV
                     DVAIYLQLTMQLREREEPVQIDLAAVRWASGTRFGVEFIKIRPEEEDRLKKFVAVLDS
                     TH"
     gene            complement(11602..12090)
                     /locus_tag="VLL06_01360"
     CDS             complement(11602..12090)
                     /locus_tag="VLL06_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29643.1"
                     /translation="MLLRHVGAPLLLACAMLAVACVKPLAPQTACMPSPPPPSSMSPQ
                     PGDRHVLVGEWEYIDGAVDGAVGRLTLDQQGNGHYNWKDGRFETHTLIGHTWRGIWFQ
                     KGNDRDGGFTVEFSPDFSKGEGCWWYSRIGEDHAPMQKGGTFRLSKKTSGISDRNTPP
                     AP"
     gene            complement(12199..>12428)
                     /locus_tag="VLL06_01365"
     CDS             complement(12199..>12428)
                     /locus_tag="VLL06_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HSS29644.1"
                     /translation="PSVPQGAYYVLADTSRLPGTTGKERAMFLLETIGLAGVPGEAFF
                     SGSDGRQFIRFSYAKIDADIDDACRRLARLG"
BASE COUNT         2748 a         3692 c         3409 g         2579 t
ORIGIN      
        1 tacccaactg ttcgacaatg gattccgccg gataggtcac gatctccgca agcgtaggat
       61 gataatgcgg catgcgcaag agatcggacg cagtcccatg ataatacatc acggcgatca
      121 attcgtggat cagttctccc gcctcagggc cgacaatctg tgcgccaagg agttcgcctg
      181 tcgtcggttc acagaggagt ttcacaaatc cgtgcgatgc gccaagacag agcgccttcc
      241 catgatcggc aaagggatat gaggcggtca aatggggaat ctttcctgcg tggcaggcgg
      301 cgtcgttgag gccgacaacg gcgacttggg gatctgtaaa gaccacctca gcatcgagac
      361 gatgatcaag ctcttttgcc ggcctgtcgg gatgtgtcac gttataggcc gcaatctctc
      421 cctgttggat agccagatgc acgataggcg tgacatcgtt cacgtcacct acggcataaa
      481 tatgtggttg cgacgtgcgc atggcgccat cgaccacaat acgcccgctg tcgacgatca
      541 cctccgccag atcaaggcga agcccttcga tgttgggccg tcggcctaag gcctgaagaa
      601 tctcctcgcc ggcgtaggac cgttcacgtc cgtccttcat cgcccagacc gtcttccctc
      661 tctcgtcgca aatcacacgg agcaattgca cgcccgtgac gacttcgatg ccttcgtcga
      721 tcaatgcggc ttccagtgcg cgcccgacat cctcgtccat ctcagaaagg agcgtccgtc
      781 cccgttgaag aattgtgacc ctcgtcccaa gtcgagcaaa aaactgaccg agctcgagag
      841 caacagaccc accacccagc acaatcaacg agtccggtag ggtgcggaga tccagaatcg
      901 tgtcgctcgt gaaatatccg gcttgggcca agccaggaat cggaacctca tccggcctcg
      961 acccggtggc caggatgaat gagccggacg acaagatggt atctccgacg acgatctcat
     1021 gaggcgaccg aaagacagcc tgcgattgat agagtgtaag gctcggatcg cggagctgct
     1081 gaatacgata gtcggcaaac tcgcggacca gccgatcctt tcgatccacg atcgcagaca
     1141 gatcggccct ggttgaaacg ggggagagcc cgaactcctt cgcccgcctc agaagggccg
     1201 caacctcggc agaacgcagg atggcctttg acggcatgca gccacgcaaa atgcagagcc
     1261 ccccaagcgg gccgtgatcc acaatcgcca cgtcggcgcc ggcatcacgc gcggtgcgtg
     1321 ctgcagcata gccagctgac ccgccgccga tgacgatgac atcgtgagtg ttgcctgtcg
     1381 aaacatgacc ctgtgatttc atcgtgtgtt ccctttatag tatggctcaa ggatggcagg
     1441 atacacgatg gtgcaacctg gtcgctatcg gcactataag ggccacgaat atgaggtgct
     1501 gggggtggct cggcactcag aaacggaaga ggaatatgta gtctatcgcg cgctctacgg
     1561 cgacggtgga ctatgggtca gaccgacgga tatgttcctg gagacggtgc tcattgatgg
     1621 tcgtccttgt ccgcggtttc gatttcttac tcctccctag gaagctctcc gagtcattta
     1681 tacccttccc ttgaaactga tgcattgtta tcctccggcc atggctggac agacgacttc
     1741 ctgccttaag tatttccagc agggatccga tacagacctc gtgaggtctg ccatgccgat
     1801 tatacgtgcc gtcaatggca tcatcgaaac ctatccttct gatccggtgt cccctcgccc
     1861 accagcgact cgggttccct cggctggtcg agaggaccat tcaaagagtc aggagcgcca
     1921 gactgctgat catgcagcac gtcttgccca acgggcctac cagcaacagg cacaccaagc
     1981 cactgaaccg aaaccggccc tcctcgcaca ggacttaatg acctcgcccg tcacgtggct
     2041 gccgtctagt agtacgctat tggaggcatg gactgtcatg acacggaagg ggattcacca
     2101 tctccctgtg acgtcgatcc atggcacgtt ggtcggaatg atatcgaatc tcgaactgct
     2161 gccctatgcg cgagagttgg aatctgtcga ttcgccggga ccgtctgccg aacacaaact
     2221 ggcccgtgtg atgaacagcc gagtcttgtc tgcgaccccg acgacagaga tccgcgagat
     2281 cgcccatatc atgctcgatg aacatgtcaa cgcgattccg atcctcgaca gctcccgtca
     2341 cccggtcggc attctgacga ccagcgatat tctccgggcc atcgtacacc gaagccccct
     2401 cgaactctgg acataggcaa ccgccttgaa tctccggggc aaccacagga ttaccgcccc
     2461 atggacagat ccgcagcatc ctgttagggc aaccatgtcc agtgcatgaa ggttgcggcc
     2521 tgcccgattc tctgtctctc tgcgtccagg acattccaga tgagggcatt ttcgacgaga
     2581 aaacggcgcc ctgttgacgt cactctcaca ccttgatagg tatcgagata gccgcgggtt
     2641 ttggcttgct ccaacatcca ttcgcgttca gcccgattgg tgggctccgc tgtcagccgt
     2701 gacggtgtcc tcgtcagatc ctcccaggtc atctcccaca gctccaatgc catctgattg
     2761 ccgtaattga tgagagggtt ttcttccatc ccgtgggaga caacgacaaa gggagagaga
     2821 aagagggcat gggcttgata ggcaggatcg cccactcgtt cgagcagttc acgcccggtc
     2881 caatgacgaa agctgttgag gagcagttgc gaccactcga tgacgtcagg atcggtccat
     2941 acctgaggaa ccatggcaga atcctccggt gcatcgtgca ggaggggcct ccaccctgcc
     3001 gaccatgcgg tggtaccacg ttcatacgtg caacgcaata atcgggtgcc actcttgtac
     3061 agaccatagg aggactctgt ataatatgga tcctctcgga agtacctctc cgagagagac
     3121 ccatcattga ggagcaccta tggcagcgca aaccctcttc gacaaaatct gggagtctca
     3181 cgtggtccgt gcagagcctg acggaaccac cctgctgtat atcgatcgtc agcttgtcca
     3241 cgaagtgaca tcgccacagg catttgaagg gctcaagctc tctggtcgca ggcctcgacg
     3301 tcctggtgcg acattggcgg tgccggatca caacgtcccg actaccgacc gaaccgtggg
     3361 tatcactgat cctctcagtg cgaaacagat ccagacgctg gaggagaact gccatgactt
     3421 cagcatcgcg ctcttcggca tgaacgatat tcgccaaggt gtggtgcacg tgatcggacc
     3481 cgaacagggc tttacccttc ctgggacgac catcgtatgc ggcgattcgc acacctcgac
     3541 gcacggagcc ttcggggcct tggccttcgg catcgggacc agcgaagtgg aacatgtgct
     3601 ggccacccaa tgcttggtgc agaaacgacc caagacgatg gaggttcgcg tcgaagggat
     3661 gttgtcgagt ctctgctctg caaaggacat cattctcgca atcatcggca agatcggcac
     3721 agccggagga accggctatg tcatcgagta cacgggctcg gcgattcgcg cgctcagcat
     3781 ggaagggcgc atgacgctct gcaacatgtc aatcgagggc ggcgcgcgcg ccgggatggt
     3841 cgcaccggac gaacatacta tcgcctatat caaaggccgt cctctggctc cgaagggaga
     3901 gctgttcgag caggccgtac acgcatggcg gcagctcaag accgatgcga acgcgacata
     3961 cgataggacc gtcacgatgc aggccgagga catggccccg caagtcagct ggggtacgag
     4021 tcccggcatg gtgactggaa tcaatcaacg agtgccggac ccgcgtacga tcaccgacga
     4081 gaaccagcgc caggcgacga tgcgggcatt ggaatacatg ggtcttatcc cgaacatgcc
     4141 cataaccgat atcaagatcg atacggtttt tatcgggtct tgcaccaatt cacgcatcga
     4201 agaccttcgc ctcgcggcca acttgaccaa gggtaagcac gtcgccaggg gagtgcgggc
     4261 aatggtggtg ccgggctcgg gactggtgaa acaacaggcg gaacaggaag ggctggaccg
     4321 cctgttcaaa gaggcgggat tcgagtggcg cgaagccggg tgcagtatgt gcctggcaat
     4381 gaatgccgac gtacttcagc cgggagagcg ctgcgcctcg accagcaatc gtaatttcga
     4441 aggccgccaa ggggccggag gtcgcacaca tctcgtctct ccggctatgg ccgtcgcggc
     4501 cgccgtcgag ggacatttcg tcgacatccg aagctggaaa tagaggacct atgcaaccct
     4561 ttaccaccat gacaggtctg gtcgcaccgt tggaccgcgt caatgtcgat accgatcaga
     4621 tcatcccgaa gcaatttctc aagacgatca agcggaccgg gctccgcgaa ggtttgttct
     4681 acgattggcg caagcaaaag gacggttcac aggatccgac gttctttttg aatcaaccgc
     4741 gcttccaggg cgcgactatt cttattacac gggataactt tggatgcgga tcgtctcgtg
     4801 aacatgctcc ctgggcgctc ctcgaccaag gattccgctg tatcattgcg tcgagctttg
     4861 ccgacatctt ctacaacaac tgcttccaga acggcatgtt gcctgttgtg ttgaaggccg
     4921 acgaggtcct ggcaatgatg gacgacgtcc ttgcgacgcc cgcgtatcaa ctgactatag
     4981 acctcgggaa ccagacggtg accaccccgg acaatactca ttatcgcttc gagatcgacc
     5041 ccttcagaaa agactgtttg tatcgaggtc tcgacgccat cggattgacg ttgcaatacg
     5101 aagctgccat cgccgcgtac gagactcgac ggaaagccga ggctccctgg ttattcacag
     5161 atcttcgcct ataacccggg gagcgcgtga tgaaaaagct gtttctggtg ctagtggtcg
     5221 tgtgcttcat tggcgctagc tatttgctca ttgccttcaa ctacagctat tccgacggca
     5281 gtcgagccgg ctacattcag aaattttccc acaagggctg gatctgcaag acgaacgaag
     5341 gggagctggc tatgaccacg gtgcccggcg tcgctccggt attgtgggcc ttttcaatct
     5401 gggataacaa agtggcgtca caactctcgc aggccatggg aaagcgattg gtgctgcact
     5461 acaaagagta tcgctacctc cccacgacct gcttcggtga gacggcctac ttcgtcgatc
     5521 gcgtcgagat cactgaataa actcaccttc ctcgcctcca acccaagccc ggattattca
     5581 tgaatgcggt gacgagtcgt tcttcccctc cgtcgcggaa accgactgac gtggtgcggg
     5641 ccgaacggcc ccacatcatc agatgatttc aatgaccaag gccttcatgc tgatcggcat
     5701 tcacgccacc atggacacac cacacttgga cgccatgggc ccaattgatg aagaccgagc
     5761 caccgcctaa gccgaaatgt acggcccacg cgcccgaggg gttatcacca accctcgtct
     5821 ccgaccaata cacggcagac tggaccccca gaaacggatg gcccggcgga agagtcggac
     5881 cgggaggagc gacggaagaa tccaccagac tgcgtaactc cggcaaagac ggcagacgcc
     5941 agccttgctg acctccgaca tttttctcgg tgcagatacg acgggccgcg ctccacctgg
     6001 ccatcgtcgt ctggggggac ttttcccaga ccaggccggt ttctttatcc agcaccgcct
     6061 cgctgttgaa cgcaggtaga atgacgaacc gctgtgccgc cggcaggact ttgccggagt
     6121 tctgggtgat gcccgacgga tcggtcggtg ccgccggcgc gtctgctgcg ccgaacaacc
     6181 caccaaacgc cagcgcacct atcgtgacgg tgaggaaccg tcgtctcgtc atcacgtcgt
     6241 ccatcccttg gtctgttgat gtagcataaa tcgcctgatt cttgatctgt gacaccaacc
     6301 ttcagccacg tggcgagggg catcatcacg tctgattgtt gcctgttcgg gtttagagga
     6361 ggtgaacaag aagcatggca ccctatttca ccaagctatt aaatccactt cttccgtatg
     6421 aaaaatccga gcatggcgat cgcgattgcc gccatcatgc cgagtaccag cggatagccc
     6481 cactcccact tcagttccgg tatgtgctcg aaattcatcc catagatacc gacaataaag
     6541 gtcagcggca tgaaaatcgt cgtaatgatt gtcaggactt tcatcacggc gttcaggcga
     6601 tagctgatgc tcgacaaata gatatcgaga ctggctgaga ccatttcgcg cagcgtctcg
     6661 atcgtgtcca caatctgaac cacatgatcg tacacatcgc ggaagaacac cttcgtcgat
     6721 tcctgtaaga gggggcattc tgatcgggag agactgttcg tcgcttcacg gagtggccac
     6781 accgcccgcc gcagaaagag caattgccgc ttgagtgcgt gaatctggtg tagcgtctcc
     6841 ggcttcggat cgctgaccac cagatcttgc agtgcttcaa tcttctcgcc taacacctcc
     6901 accactgtga agtactgatc gacgatcgcg tcgatcagcg catacagcag gtaatcagcc
     6961 ccggattgcc gcaggcgtcc tttccctcca cggagacgat cccgcaccgg ggtgaagaca
     7021 tcggccccgt tttcctgaaa ggacaacaca aagttccgtc caaggacgag actcacctgt
     7081 tcgacgacga tatcctgtcg atcggtaact gagagcatct tcatgaccag gaacaagtag
     7141 gtgtcatagt cgtcgagttt cggccgctga tcggtgttgg caatgtcttc aaggagaagg
     7201 gggtggagct ggaactgttt tccgaatgat tccagaatgt ccatattgtg aatgcccccg
     7261 acatccaccc acgccacgga cccatcggtc gggggcgcga gttcagcgag cgacaggtcc
     7321 cggcgctcat cgcagcgggt accggcgtag ttgaatgccg taatcgtcac cttgtccgtt
     7381 tttttctcgc cgatatggac aagggtcccg gggggaagtc ccgtctttct ggatcgtttc
     7441 tgtaccagct tcatcggttg tgacctcgca ggaccccctg cttgtacgca tgttctcacc
     7501 gagggtcaag aggaccgtcg gcgccagtcc acagcggctg tctttccgct ttgccgatcc
     7561 ggtaagacct gctactcttc ttcttccact ccgcgaggca tgcacgatgg accgggagct
     7621 cagtacgttg gtcgaagcga tccggaaggc cgccgtgaag gtcggtgaaa tggtcaggga
     7681 tggtttcgaa atccagacca agccagacca ttcgccggtc accaccgtag acctcgaggt
     7741 caaccgaatc ctgcatgaga tgcagggccg agagtttcca agggatggat ggctctcgga
     7801 agaatccccc gacgatccag cccgactcac ccatgcgcgc gtgtggattg tcgaccccat
     7861 tgacggcaca aaggccctgg tgaatggggt gccggagttc tgcatttctg ccgccttggt
     7921 cgaacgaggc gcaccggtca tcgccgcaat cctcaatccc tcaaccgatg aacttttcac
     7981 tgcagtgcgc ggcggcggac tgttcttgaa cggcacacct ctgacccact cgctccctca
     8041 tgacttcaat cctgtcatca tggtcaatgc gtgggaactc cggatcggtc gatggtcgac
     8101 gctcgccgag acctctcggt gccgccctat gtactccatc gccaatgcac tggccctggt
     8161 cgcagccggt cgtgtccagg ctgccctcac catcgagccg gaaaacgaat gggatctcgc
     8221 ggccggagtc ctgctcatcg aagaaggcgg aggcacgatc agcgatgcag ctgggaagcc
     8281 ttttgttttc aatcaaccga caccgatgtt tcatggtgtc atcgctgtcg ccgccacggc
     8341 cgacaaaggt ctgcgtgcca aactccaggc ccatgcagaa catgccctct cgcatccacc
     8401 gcgaacgtga gttgatcctt gactatcgca ctcttgggaa ccggtctgtt gggtcgagcc
     8461 attgccgaac ggcttcagtc ggtcggccat actgtcacgg cctataaccg aaccacgtca
     8521 aaggcgcttc ccctccaggc ctgcggcatc accgtcgtca cccgaccgga ggaggcgatc
     8581 gctcaagccg actgtgtggt cctcatgctg gccgatgcag cggcaatcca ggccgtcctg
     8641 ctgacaccgg cctcgttggt ggtccttcgc ggtaaaactg tgatccaaat gggcaccatc
     8701 gctcaggacg agagcctggc cctgcagcat gagatcgaac aagtcggcgg atcgtattgc
     8761 gaggccccgg tcctgggaag ccttgcggaa gcacaagccg ggacactttt gatgatggtc
     8821 ggtgggacgg agggacagtt tgtccagtgg ggtccactgt ttcgtgcgct gtgtcgggag
     8881 ccgcgtctaa tcggtcctgt cggaaaggcc gcgtccctga agctcgcgct caatcagttg
     8941 attgttgcgg aaatatcggc gttcgcctta agccttggat tggtccagcg agcgggtgtt
     9001 cccgtagaca cttttatggc catcttgaga gaaagcgcct tgtttgcccc ggcgttcgag
     9061 aagaaactgc cgcgactcct gacccgggac tatcagcatc caaacttttc cacacgtcat
     9121 cttctgaagg acgcggagct cttcctcagg gaagcctccg gttacgcatt gacgacgagt
     9181 ggtctggaag gaatcaggcc attactggaa cgaaccatcg aacaggggct tggtgattcc
     9241 gattattcag ccatcttcga gatcatcaac cccgatcaat agtcttacca tcccctggtt
     9301 tattgccacc ttcccacatg agttagcccc gagaaggttt gtaccccttt ttgatattca
     9361 ttgtgaacgg taaaaatact tctatactta ctattgtcgc ggccggccaa caatacactc
     9421 aaacggacat cgtgttcccc ccatgaccta cgcgatagga acaatgcaga agtcggccca
     9481 gaaaaccatc gaaccccgtg cctatgatcc tttgatccac gagggcttca tccgccaggc
     9541 ggatctcgtg aaagcggttc aggaatccct gcagggggtc accgacctgg aaggcctcct
     9601 gatcgagaag taccgcatcc cgaaagcggc actcggcaag acattgagcg cgttcttcga
     9661 ctgcccctat attccgtacg acaaacggac cctggctgat cctcaactcc tcaataatct
     9721 cagtcacgat taccttcgta ggcaccactg gctcccgctg aagcgacagg gtgacatcct
     9781 ggatgtcctg atcgacaacc cccatgatct tgacagaggt catgacatcc agcgcgcctt
     9841 ccccggcctg acgattcggt atgccttggg ccttcggtgt gatattgagc ggttcctcag
     9901 cgccacgatc ggagaaaccg acagtgggtc cattgcggac acgttgggtg agctggtcaa
     9961 cgatgctcac cacgagcaag ctctcgacaa cgactctgac ggcattgacg aaaacgattc
    10021 cgcgatcgta cggcttgcga atcagattat tgtcgaagcc taccgacaag aggcgtctga
    10081 cgtccatatc gagccctact cggatcgcaa ggaaacggcc atccgttttc gcgtggatgg
    10141 tacctgttcg acctatatgc ggattcccgc gatctatcgc cgagcgttgg tgtcccgtat
    10201 caagatcatg tccagtttgg atatttccga acgccggaaa ccgcaggacg ggaagatccg
    10261 tttcaagctg aaccaggatc gagaaatcga gctgcgcgtg gcgacattgc cgactgccgg
    10321 caacaacgaa gacattgtca tgcggttact cacggcgaag gaacccctcc cactcgatgc
    10381 cttggagttt gctccggcca cgctcaccac gctcctggaa atcacggaga aaccacacgg
    10441 cattatctta tgcgtaggcc ccaccggatc cggaaaaacg acgaccctgc atgccctgct
    10501 gcaccatctc aacacggatg aacggaaaat ctggacggcg gaagacccca ttgagatcac
    10561 ccaggaaggc ctccggcagg tgcaggtcca tcccagaatc ggctttacct ttgcctcggc
    10621 catgcggtca ttcttacggg ccgacccgga tgtcatcatg atcggtgaga tgcgagataa
    10681 agaaacagcc gatgtcgcca tcgaggcctc gttgaccggc cacctggtct tgagcacgtt
    10741 gcacacaaat ggagccgtcg agactgtgat ccgcctcctc gacctggggt gcgactcatt
    10801 caactttgcc gatgctatgc aaggggtgct cgcgcaacgc ctctgcaagc ggatctgcgt
    10861 caattgcaag gagccttacc atcccacggc gcaagagttc gataaactcg tgcatgggta
    10921 cggtaaacac gcctggaatg tgcttggagt ggggtatcgc gaggactttc agctgtatcg
    10981 agggcgtggg tgtgacacct gtaagcacac aggcctcaaa ggccggatcg ccttgcacga
    11041 actcctccgc ggttcggatg aactgcggaa cctgatccag acccgcgcaa aaacgatgga
    11101 aatgcagagc cttgctgtaa aagaaggaat ggtgacactc gtgcaggatg gaattctgaa
    11161 agtgctcgcc ggcctgacca cctatgatca agtccgggcc gtcgccatga aatagcggct
    11221 cgaatctcgc tgcagtctcc tgcactcagt gtgttgagtc tagtactgcg acgaatttct
    11281 tcagtcgatc ctcctcttca gggcgaatct taataaactc cacaccgaac ctcgtcccgg
    11341 aagcccatcg aactgcggcc agatcaatct gaaccggctc ctctcgctcc cggagttgca
    11401 tcgtcagctg taaatatatt gctacgtcga cgacagcctc cgacgtgatg gcgcacccct
    11461 ccgcagacac gttcaagatc gtgcctccat gggaaggctg gtgttccccg aacgacgcag
    11521 gaagttgcac ggcaaaacgg gtatgcttac gtccttccat gggactcctt actgatcatc
    11581 ggctctggta tgagaagctc ctcacggtgc cggtggagta ttgcggtcgc ttataccaga
    11641 agtctttttg ctgagacgaa acgttccgcc tttttgcatg ggtgcatggt cctcgccgat
    11701 acggctgtac caccagcatc cttccccttt tgaaaaatct ggcgagaact cgaccgtaaa
    11761 tccaccatct cgatcattcc ctttttgaaa ccatataccg cgccaggtat gaccaatgag
    11821 cgtgtgtgtc tcgaatcgtc cgtctttcca attgtagtga ccgttgccct gctgatcgag
    11881 ggtcagccga ccgacggcgc catctaccgc gccatctatg tattcccatt cacccactag
    11941 gacatggcgg tcaccaggtt gaggcgacat cgatgaagga ggaggaggag acggcatgca
    12001 cgcagtctga ggggccagcg gcttgacgca cgccactgcg agcattgcgc aggcaagaag
    12061 gagcggggca ccgacgtgtc gtaggagcat gttggtcgta tcactcttat cgcttcttct
    12121 tacttaccat ttggtgaacg gctagacaag cagtcacgcg aagaatcgga cgcggcgcag
    12181 gaggcctgag gccaactcct aaccaagccg cgcaagccgc cggcacgcgt cgtcgatatc
    12241 cgcatcgatt tttgcataac tgaatcgaat gaactgcctc ccgtcagacc cggaaaagaa
    12301 ggcctcaccc ggcaccccgg ccaggccgat cgtctcaaga agaaacatcg cccgctcttt
    12361 tcccgttgtg ccaggcaggc gcgacgtatc ggccaataca tagtaggcgc cctgcggaac
    12421 cgacggcg
//
LOCUS       DATIPF010000051         1950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_184540, whole genome shotgun sequence.
ACCESSION   DATIPF010000051 DATIPF010000000
VERSION     DATIPF010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184540"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(623..>1950)
                     /locus_tag="VLL06_01370"
     CDS             complement(623..>1950)
                     /locus_tag="VLL06_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HSS29645.1"
                     /translation="ETPDGPYPYAGVPWFSTPFGRDGLITAFEFLWVNPNIARGVLAY
                     LAANQAKEMVAEQDAEIGKILHETRRGEMAALNEIPYGRYYGSIDATPLFIMLAGAYY
                     ERTGDRAFISQIWPNIELALQWIDRAGDADGDGFVEYRRRSKGGLIHQGWKDSEDAVF
                     HADGSSAEGPIALCEVQGYVFAAKRRAARLALALGHPEQARRLLKEADTLRTQFERRF
                     WCNDLSTYALALDGFKRPCRVRTSNAGHCLWTGIAEYKHGVKLAKLLVGDEFFNGWGV
                     RTVAATETRFNPMAYHNGSVWPHDNALIAAGMASYGFKEGALKILCGLFDASLFLELH
                     RLPELLCGFSRRPGEGPTLYPVACSPQTWSSVALFLLLQACLGLRIEAPRARLSFLQP
                     VLPPFLDHIEIQHLRVGDAVVDLSLERHATDVGINILRREGRVEVVVTK"
BASE COUNT          463 a          570 c          517 g          400 t
ORIGIN      
        1 ccatggtcgt tcacatcagg catggcatcc tcgttcgatt tcatctgccg ctaactccag
       61 agggttgcgg caatagctgc agcgaccccc aagtatcccg ttccggccgt cgcggctcac
      121 gcgaaagaca agaacgtacc ggtgactgcc gcaatgagca cagagagaac cttttaggtc
      181 gcgcaggact ggcaatcctc cgaccatgag gtccctctgc gcctcggaac atgtcattga
      241 atcggtgtga tgtctttcct ccattcgtga ccctctcttt aatatcccaa tatcgaaatg
      301 gctcctcagc agtacgtcat atgacacagc cgatgccgtg atggtgagca agactcagac
      361 cgcccaatct gattggaacg tcgcatcgga taaccatcaa tttttacaat gagtagagat
      421 ctctggctgc ttcgccgtgg ggcatataac cggcgctgaa tatcgacttg caggcggact
      481 agttgcatcc gacagaacgc taggttcaac acaagtatcg attacatata gaaattgtgg
      541 gagaaagaga catcttgtcc ggcaaggaca agatgtctcg atgatcacaa cgtctgaata
      601 atacaggagg gcttgcgttg gattacttcg tgaccacgac ctcgacgcgg ccttcgcgcc
      661 tcaggatgtt gattccaaca tccgtcgcat gacgttcaag cgacaggtca acgacagcat
      721 ctccaacccg gagatgctga atctctatat ggtccaggaa gggcggcaag accggttgca
      781 aaaaagacag gcgggcacgc ggagcctcaa ttcgcaaacc gagacaggct tggagaagca
      841 ggaacagggc gaccgaggac caggtctgag gactgcaggc gactgggtac aacgtggggc
      901 cttcaccggg acggcgcgag aacccgcata acaattcagg caaccgatgc aactccaaaa
      961 ataggctggc atcgaagaga ccgcagagaa tcttcaatgc gccttctttg aatccgtagg
     1021 aagccatgcc ggctgcgatg agcgcattat cgtgcggcca gaccgatcca ttatggtatg
     1081 ccattggatt gaagcgggtc tcggtggcag cgacggtgcg gacaccccat ccattgaaaa
     1141 attcatcgcc gactaggagt ttggccaatt tcacaccgtg cttgtattcg gcaataccgg
     1201 tccagaggca gtgcccggca ttcgacgtcc tgacacggca gggccgcttg aacccgtcca
     1261 aggctaatgc ataggtcgag agatcattgc accagaatcg tcgttcaaac tgcgtgcgta
     1321 gtgtgtccgc ttccttgagg agacgcctgg cctgctccgg atggcccagc gccagggcta
     1381 gtcgagctgc acgcctcttg gcggcaaaga catagccctg cacctcgcag agcgcgatcg
     1441 gcccttcggc cgagctgcca tcggcatgaa acacggcatc ctccgaatct ttccaccctt
     1501 gatggatcag cccgcctttc gacctgcgtc tgtactccac gaacccatca ccatcggcgt
     1561 cccccgcacg atcaatccac tgtaatgcca gctcgatatt cggccagatc tgtgagatga
     1621 aggcccgatc gcccgtccgc tcgtaatacg ctccagccaa catgataaac aacggcgtcg
     1681 catcaatgct gccgtagtag cggccgtatg gaatttcatt gagtgctgcc atctctcccc
     1741 ttcgcgtttc atgtaaaatt ttcccaatct ccgcatcctg ttccgcgacc atctccttcg
     1801 cctgattcgc agccagatag gccaacacac cccgggcgat attggggttg acccacagaa
     1861 attcaaacgc ggtgatcagg ccgtcacgtc cgaacggggt gctgaaccag gggacaccgg
     1921 catagggata gggaccatcg ggagtctccg
//
LOCUS       DATIPF010000052         4928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_713501, whole genome shotgun sequence.
ACCESSION   DATIPF010000052 DATIPF010000000
VERSION     DATIPF010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713501"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(326..1108)
                     /locus_tag="VLL06_01375"
     CDS             complement(326..1108)
                     /locus_tag="VLL06_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HSS29646.1"
                     /translation="MYEWSLYLLVGILGALIGSFLNVCIFRLPRGESIAWPGSHCPSC
                     AHPIAFYDNIPLLSYLWLGGRCRACRAPISIRYPIVEATNALGYLTILWSFGPSWTAA
                     LYALLFSALVVVTGTDLTHKMIPNVITLPGAVVGLLGAVTVLPVGVINSVLGLTVGGG
                     LLWLLAWLSPYLFGKEGMGGGDIKLLAMIGAFLGWKPALLTIMIGSLTGSIIGVSLIA
                     LRIIKRDDYIPFGPFLVLGALLSMFFAQPLLDWYQGLLGPAD"
     gene            1329..1604
                     /locus_tag="VLL06_01380"
     CDS             1329..1604
                     /locus_tag="VLL06_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HSS29647.1"
                     /translation="MTRTAKTKTKPSLANLIPPPRKKRPESLTSREQEILELIWAGFK
                     NKEIGQRLKISVKTVEAHRANMMKKMRVSNTAQLLKTAIQDGTIKIR"
     gene            complement(1591..2382)
                     /gene="rlmB"
                     /locus_tag="VLL06_01385"
     CDS             complement(1591..2382)
                     /gene="rlmB"
                     /locus_tag="VLL06_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HSS29648.1"
                     /translation="MAPAGGSDSQPELIYGLHAVREALRAGTRPLQRLLVLRTDRQFG
                     DLVQSAKAKRIPVHIEPPPAFDRLVPTGNHQGVIALIAAKPYDTTEQILDQAKTRGEK
                     PFLVLLDGVEDPHNLGAVLRTAEAAGVHGVFIPERRAVGLTSVVAKVSAGALDHISVG
                     RVGNLIRLIQDLKEAGLWVYGVDPEAEKLHTDIDMTGPIALVLGGEGEGIRPGVLGEC
                     DDRIRIPMQGRVQSLNVSAAAAVMLFEVVRQRRKGVVPPAKATES"
     gene            complement(2357..3790)
                     /gene="cysS"
                     /locus_tag="VLL06_01390"
     CDS             complement(2357..3790)
                     /gene="cysS"
                     /locus_tag="VLL06_01390"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HSS29649.1"
                     /translation="MLSIYNTLTGKKEPFHPLQPKIVRMYVCGVTVYDYSHIGHARSA
                     LVFDVIRRYLESTGYQVEFVKNFTDVDDKIIKRANEQGVSCETLTAKYIQAYYEDMER
                     LGIRQASKEPKATEHMTDIVRLTETLVKKGLAYQVDGDVYFEVAKYSGYGHLSKRKLE
                     DLQAGARVEVDERKRSPMDFALWKRSKPGEPSWESPWGPGRPGWHIECSAMSIKYLGE
                     TFDIHGGGMDLIFPHHENEIAQSSGATGKEFARYWIHNGFVQTNQEKMSKSLGNFFTI
                     REIFEKSKWSQDVTGEVLRYFLLSTHYHGPLDFSGQALEEAKQALEGFYGLFNRLCES
                     ESSTVGDKDLPSSVDRTRESFRKAMENDFNTPVAIAELQRFRSDVNKLLDIGLSTQGR
                     QQARQAFRMLGGVLGLFQLDLWEYGMNLGSGQYQISGEEIDLKLVERNEARKQKNFKK
                     ADEIRQFLASRGIVIEDKPDGTSRWKR"
     gene            complement(3798..4439)
                     /locus_tag="VLL06_01395"
     CDS             complement(3798..4439)
                     /locus_tag="VLL06_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HSS29650.1"
                     /translation="MVGSLIEAIITELSRFVVSMISTFGYTGIFITMAIESACIPLPS
                     EIIMPFSGYLVTTGQFTMLGVTLAGAIGNVAGSLVAYYAGVWGGRPFVERYGPYVLVS
                     RKDIEMADRWFAKYGDAAVFFSRMLPVIRTFISLPAGIARVNIYRFILYTFLGSLPWC
                     YLLAYIGVKMGEQWEHLRDYFHQFDIVIGLCLALAVGYFLWSHWPRRRITPEL"
     gene            complement(4452..>4928)
                     /locus_tag="VLL06_01400"
     CDS             complement(4452..>4928)
                     /locus_tag="VLL06_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HSS29651.1"
                     /translation="SAAMEGTILGVPSLAVSQQGQEHFYFDAGARYAVRIARLILKEG
                     LPDETLVNLNIPNRPYGSITGVRVTCLSRRRFDNPITEKVDPHGRTYYWIAGTRVSWS
                     RSKDADHEALEQGAVSLTPIHLDTTNHGVLDRFRKWETVRRPHAGRVAPSSKSKGK"
BASE COUNT         1217 a         1413 c         1261 g         1037 t
ORIGIN      
        1 caatacgatg agaaactcgg tcagagtcca accctgttcc tgtatctgtc gctttcttgg
       61 catgcccact cccatagtag aattccactt acgaggatga gcgcagtctc caagacccgt
      121 gcccaagttg aggcacgccc agatgactga atgttttcgt gaggagccgg aacggaatac
      181 ttcgcaggtc cgcgcgtttt cgcgtgatca cgagaaaacc tggaagcaga acaggcttgt
      241 gcactggtca agaaggaaga agaaaaccag agaggtcgct cactataaga tacagggatt
      301 tgcccgaaga gatacagaga gattgtcagt cggcggggcc aagaagacct tgataccaat
      361 cgaggagcgg ctgggcaaaa aacatggaga ggagggcccc caacacaagg aaggggccaa
      421 agggaatgta gtcgtcacgc ttgatgatgc gcagagcgat taagctgacg ccgatgattg
      481 aaccagtgag cgagccgatc ataatggtga ggagtgcagg cttccagcca agaaacgcac
      541 cgatcatcgc cagcaatttg atgtcaccac ctcccattcc ctctttgccg aaaagatagg
      601 ggctcaacca agccaggagc caaaggaggc cacctcccac ggtgagaccg agtaccgaat
      661 tgatcacacc caccggcagg acggtgactg cgccaaggag tccgaccacc gctccgggca
      721 gggtaatgac attggggatc atcttatggg taagatccgt tcccgtcacc acgaccaacg
      781 ctgaaaataa cagggcatag agggcggccg tccagcttgg accgaaggac cataagatgg
      841 tgagatagcc aagggcattg gtggcttcaa cgatggggta gcgtatcgaa attggcgcgc
      901 ggcaagcccg gcaacgtcca ccaagccaca ggtagctcag cagaggaata ttgtcgtaga
      961 acgcgatagg gtgggcgcac gaggggcaat gggaaccggg ccaggcgatc gattcgccgc
     1021 ggggcagcct gaagatgcac acgttgagaa aactaccgat caacgcgccg aggatcccga
     1081 ccagaagata gagagaccat tcatacataa tcgactcagg aacagactcc ggctgggaac
     1141 acccgagtcg tttcacatgc caattgaaat aaattaggta acttcactct ggccgatatc
     1201 ctctagttta cttatggcga gagagccctt ataatttgtg gcttgcgggg ctagggttta
     1261 tccttgggcg cttctccgtc acagtgaatt ataccgtact ctgttattcg ttaaaggaga
     1321 acaagataat gacacggact gcaaagacga aaaccaaacc ttctctggcc aatctgatcc
     1381 cgccgccccg caagaagcgg cctgaatcgc tcaccagccg ggagcaggaa attcttgaac
     1441 tgatttgggc tgggttcaag aacaaggaaa tcggtcaacg gctcaagatc agcgttaaga
     1501 cggtcgaggc gcatcgggcc aatatgatga agaagatgcg tgtgtcgaac accgctcagc
     1561 tgctcaaaac cgcgatccaa gacgggacga tcaagattcg gtagccttcg ccggcggcac
     1621 gacgcctttg cgacgctgtc gaaccacttc gaacagcatg acggccgcgg cagccgaaac
     1681 attcagcgac tgcacgcggc cttgcatcgg aatcctgatg cgatcgtcgc attcccccag
     1741 cacacctgga cgaatgcctt ctccttctcc cccaagtacc aacgcgatcg gtccggtcat
     1801 gtcgatgtcg gtatggagtt tttccgcctc tggatcaaca ccgtacaccc acagacctgc
     1861 ctctttgagg tcttgaatca aacgaatcag attgcccacg cgcccgacgg agatgtgatc
     1921 gagcgcccca gccgacactt tcgccaccac cgatgtcagg ccaactgcgc ggcgttctgg
     1981 gataaacacc ccatggaccc ccgccgcttc agctgtccgc aacaccgctc ccaaattgtg
     2041 tggatcttcg actccgtcaa gcagcacgag aaatggcttc tcgcctcgtg tcttcgcttg
     2101 atcgagaatc tgctccgtcg tgtcgtaggg tttcgcggcg atgagcgcga tcacaccctg
     2161 gtggtttcca gttggcacca aacgatcgaa cgcgggcggc ggttcaatat ggacagggat
     2221 tcgtttcgct ttagccgact ggacaagatc gccgaactgt cgatcagtcc tcagaaccag
     2281 taggcgctgg agaggccgtg tgccggccct caaggcctcg cgcacagcat gcagcccgta
     2341 aatcaattcc ggctgactat cgcttccacc ggctggtgcc atcgggcttg tcctcgatga
     2401 caatgccgcg ggacgcgagg aactgacgaa tctcgtcggc cttcttgaaa tttttctgtt
     2461 ttctggcttc attgcgttca acaagcttca agtcgatttc ttcacccgag atctggtact
     2521 gtcctgatcc aaggttcatc ccatattccc acaaatctag ctggaacaat cccaacacgc
     2581 cgcctagcat acgaaacgcc tggcgcgcct gctgcctccc ttgcgttgaa agtccaatat
     2641 caagcaactt gtttacgtca ctacgaaacc tctgaagttc ggcaatcgcc actggcgtat
     2701 taaaatcatt ctccatcgct ttccggaacg actctcgagt tcgatcaaca gacgacggga
     2761 gatccttgtc gcccactgtg ctgctttctg attcacagag tcgattaaac aatccataga
     2821 acccctccaa tgcctgcttc gcttcctcca aagcctggcc ggaaaaatcc agtgggccat
     2881 ggtaatgagt cgaaaggagg aagtatctca ggacttcacc cgtgacatcc tgcgaccatt
     2941 ttgacttctc aaaaatctcc cgaatcgtaa agaagttccc gagcgactta gacatcttct
     3001 cctgattggt ctgcacaaat ccgttatgga tccaatagcg agcaaattct tttcccgtgg
     3061 caccggatga ctgcgcgatt tcattttcat ggtgcggaaa aatgagatcc atccccccgc
     3121 cgtgaatatc gaatgtctca cccagatatt tgatcgacat ggccgaacac tcgatgtgcc
     3181 agcctggacg ccctggcccc catgggctct cccatgacgg ctcgccgggc ttgctccttt
     3241 tccaaagagc aaaatccatc ggagatcgct tgcgctcatc gacctccact cgagccccag
     3301 cttggagatc ctcaagcttg cgctttgaga gatgtccata tccagaatat ttggccactt
     3361 caaaatacac gtctccatcg acttgatagg ccagtccctt cttcaccaag gtctcggtca
     3421 aacgaacaat atcggtcatg tgttctgtcg ccttgggctc tttggaggct tggcgaatgc
     3481 ccagtctctc catgtcttcg tagtaggcct gaatatattt cgcggtgagc gtctcgcagc
     3541 tgacaccttg ctcgttcgca cgcttgataa tcttgtcatc cacgtctgtg aagttcttga
     3601 cgaattcaac ctggtagccg gtggactcaa gataccgtct gatcacatcg aacacgagcg
     3661 cactgcgcgc atggccgata tgactatagt cgtagaccgt cacaccacag acgtacatcc
     3721 ggacgatctt cggctggagt gggtgaaacg gttctttctt ccccgtgagc gtgttgtaaa
     3781 tcgacagcat ggacaggtta caactccgga gtgatgcggc gcctcggcca atgggaccag
     3841 agaaaatagc ccacggcaag cgccaggcag aggccgatca cgatgtcgaa ctggtgaaaa
     3901 taatcccgca gatgctccca ctgctctccc attttcacac cgatataggc cagaagataa
     3961 caccagggca atgaaccgag aaatgtatag agaatgaagc ggtaaatatt cactctggcg
     4021 atgccagccg gcaaggagat gaaggtccgg ataaccggaa gcatccggct gaaaaagacg
     4081 gctgcatctc catatttcgc gaaccatcga tcagccatct cgatatcttt tctcgacacc
     4141 agcacatacg gtccgtatcg ctcgacgaaa ggccggcctc cccatacccc ggcatagtag
     4201 gcgacaagag accctgccac atttccaatg gcgccggcca acgtcacccc aagcatcgtg
     4261 aactgtcccg tcgtcacgag atatccagaa aaaggcatga tgatttcgct gggcaacgga
     4321 atgcaggcgc tctcgatggc catggtaatg aagatgccgg tgtagccgaa tgtcgagatc
     4381 atcgacacga caaaccggct gagctctgta atgatggctt cgatcagact ccccaccaca
     4441 ctatcactcc tctatttgcc cttcgattta gacgatggag ccactcgacc tgcatgtggc
     4501 cggcgcaccg tttcccactt ccggaatcga tccaacactc catgattcgt cgtatcgaga
     4561 tgaatgggag tgagcgacac cgccccttgt tcaagcgcct cgtggtcggc atccttgctt
     4621 cggctccacg acacacgggt cccggcaatc caatagtagg tccgaccatg cggatcgacc
     4681 ttctcagtga tcggattgtc gaagcgcctg cggctgagac aggtgactcg gactccggtg
     4741 atcgacccat acggccgatt cggaatattg agattcacga gcgtttcatc cggcaaacct
     4801 tctttcagaa tcaaacgagc gatacgaacg gcgtatcgag ctccggcgtc aaaataaaaa
     4861 tgttcctgcc cctgctgtga cacagccaga gatgggacac cgaggattgt cccttccatc
     4921 gccgccga
//
LOCUS       DATIPF010000053         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_803851, whole genome shotgun sequence.
ACCESSION   DATIPF010000053 DATIPF010000000
VERSION     DATIPF010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803851"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="VLL06_01405"
     CDS             complement(<1..1173)
                     /locus_tag="VLL06_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HSS29652.1"
                     /translation="MKPINEDRLLTPAYPAEGPIAPTALPANRFLSLRTKFVLYFTLI
                     LIIACSAMSWFFIENRRTAMASRLQQLGTFLLTSVVSNEHFQYAGLVAEDRTTLQQFI
                     EGFVAIEDVVYVVITGSDGTVLAQHTKGARQSSSSLVRSEDYPLYPDSQTAKQLFQSP
                     TTTPIITLLSTSNTLSTGFALEEIVYDFAMPVLRMRKGNTSLPPFSLEMEEGNTSSSP
                     AQPALVSGVIQIGLTDARLKLELATVIRNILLLTTLIIIAGALGAHLLTLRITKPLRS
                     LGVMARQVAEGHSPPPLTPATRDEVGQLTSVFNLMTHSLQERSLAITANLATIKNHVG
                     QLTTLHQVSAAIVGTLDRGQLLSTVLQLLIENLGFTRMFLMLRHRDRETAYVAQVAG"
     gene            complement(1160..2128)
                     /locus_tag="VLL06_01410"
     CDS             complement(1160..2128)
                     /locus_tag="VLL06_01410"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSS29653.1"
                     /translation="MNQTNQIHRLFRAGTSLGVRVAVGLALLLSLCTLPVSESAAIDI
                     AILQSSDIAAYREAIAGLKATGPIGAIYTEYDVQGDLELGKKLARKVRASNASLVVAV
                     GLKAALAAKVEIVDVPIVYMMVLDPLKHQLTTDNMTGTLLEVPLDRQLKIMRTFLPNL
                     RQLGTLYDPAKTSSRVKDAERQAANSDFQLKGVPVESEKDVPQQLRALLADVEVLWLM
                     PDSTVLTNESIRFILESALARQIPVIGFSPEFTRLGSLLSMSVNYGDVGRETGLLAKR
                     VLNGDGQLPLAPVPIERLKITVNLKTAKFLGITFPKELTSLIDETY"
BASE COUNT          499 a          611 c          696 g          539 t
ORIGIN      
        1 cccggcgact tgggccacat acgccgtttc tcgatcgcga tgccgcaaca tcaagaacat
       61 ccgtgtaaac ccaagattct cgatcagaag ctgcaggaca gtgctgagga gctggcctcg
      121 gtccaatgtc ccgacgatgg ctgcgctgac ttgatgcaac gtcgtcagtt gcccgacgtg
      181 atttttgatg gtggccaggt ttgcggtgat ggcgaggctt ctctcctgta acgaatgggt
      241 catcaggtta aagacgctgg tcagttggcc gacttcgtcg cgcgtggcgg gtgtgagcgg
      301 cggaggcgag tggccctcag ccacttgtct cgccataacg ccaaggcttc tcagtggttt
      361 cgtgatgcgg agggtgagca agtgggcgcc gagggcgccc gctatgatga tgagggtggt
      421 gagaaggaga atatttctga tgaccgtcgc gagttcgagt tttaagcgtg catcagtcaa
      481 tccaatttgg atgacgccgg acacaagtgc cggctgtgcc ggagaggagc tggtgttgcc
      541 ttcctccatc tcaagagaga atggagggag cgaggtattt ccttttctca ttcggagcac
      601 cggcatggca aagtcgtaca cgatttcctc caacgcaaaa ccagtgctga gtgtgtttga
      661 ggtggagaga agtgtgatga tcggagtggt ggttggcgat tggaagagct gcttcgcagt
      721 ctgactgtcc gggtagagag gataatcctc ggaacgcaca aggctggagg aagattggcg
      781 ggctcccttc gtgtgctggg ccagcacagt gccgtccgag ccggtaatca ccacatacac
      841 cacatcctcg atggccacga agccttcaat gaattgttgg agcgtcgtgc gatcttctgc
      901 cacgagacct gcatattgga aatgttcgtt gctcaccacg ctcgtgagga gaaacgttcc
      961 tagctgctgg agtctgctgg ccatcgcagt tcgccgattt tcgatgaaaa accagctcat
     1021 agccgaacaa gcgatgatga gaatgagggt gaaatataat acgaatttcg tgcgcagact
     1081 gaggaaccgg ttcgcaggca atgccgtggg agcgatgggt ccctctgccg ggtacgcagg
     1141 agttaaaagc ctgtcctcat taataggttt catcgatgag actcgtcagc tccttcggaa
     1201 acgtaatgcc caggaatttc gcggtcttga gattgacggt gatcttgagt cgctcgatcg
     1261 gcacgggggc gagcggcagc tgtccgtccc cgttgagaac gcgcttggcg agcagtccgg
     1321 tttcccggcc gacatcgcca tagttgacgg acatgctgag cagcgatccc aggcgtgtga
     1381 attccggtga gaacccgatc acgggtatct gacgtgcgag ggctgattca aggatgaatc
     1441 gtatggactc attcgtcaac accgtcgaat ccggcatgag ccagaggacc tcaacgtccg
     1501 cgagcaatgc ccgcaattgt tggggcacgt ccttttcact ttcgacggga acccccttca
     1561 attgaaagtc ggagttggcg gcctgcctct ccgcatcctt cacccgggag gaggtcttgg
     1621 cgggatcgta gagcgttccg agctgtcgga ggttcggtag gaacgtccgc atgatcttca
     1681 gctgtcgatc taacggaacc tccaggagcg tgccggtcat gttgtcggtg gtgagctggt
     1741 gtttcaatgg gtccagcacc atcatataga cgatcgggac atccacaatt tctacttttg
     1801 ctgccaaggc ggctttgagc cctacggcga cgaccagtga cgcattcgat gcccgcacct
     1861 tgcgcgcaag ctttttcccc agttccagat cgccttgcac atcgtattcg gtatagatcg
     1921 ctccgatcgg acccgtggcc tttaatccgg caatggcctc gcgataggcc gcgatgtcgg
     1981 aagattgcag gatcgcaatg tctatcgccg cggattcgga gacaggcagg gtacagaggc
     2041 tgagcaacag ggcgagtccc accgctacac ggactccgag agaagtccct gcgcggaaaa
     2101 gcctgtgtat ctggttcgtt tggttcatct ggtttgtctg gtctatctgg ttgatctaac
     2161 ttgtttcgtg tatctggttc atctggttcg ttctgttcaa tcaactaaac cagacaaacc
     2221 aaacaaacga gacaaaccag atcaacaagt ctgttctcgt gcttcacgcg ccggaatctg
     2281 tggcgcggac cgttcccaaa atatgcaagg cccttccgtc ccgatcacga gcaatctcac
     2341 ctatc
//
LOCUS       DATIPF010000054         8923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_980372, whole genome shotgun sequence.
ACCESSION   DATIPF010000054 DATIPF010000000
VERSION     DATIPF010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8923
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980372"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VLL06_01415"
     CDS             <1..985
                     /locus_tag="VLL06_01415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSS29654.1"
                     /translation="LGELAVAAWGWLGVHSYAELLLVAIFLGIGGASFAVTLPLAGRT
                     YPAAHQGLVLGIVASGNVGTVLVLFFAPRIAATGGWHDVFGWMMLPIVLAFTVFVLLV
                     RDDRQSRRAERDAQWWHSVAVMIRHPKAYWLCGLYAVTFGGFVGFCSILPLFFHEQYQ
                     VNGIVAGSIVALCGLVGSLIRPFGGYAADRIGGLRTLILSLPLIVGIIAVLGDVTGVT
                     AAVVLMVVAVGTMGVGNGVILQLASEWFPKQIGLASGIVGAAGGVGGFLLPLWLGTLK
                     EVTGSYRTGLWMFAVATGVAWGTVLVAERRARRLIKPMAVAGRVSIDRDGR"
     gene            1070..2278
                     /locus_tag="VLL06_01420"
     CDS             1070..2278
                     /locus_tag="VLL06_01420"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HSS29655.1"
                     /translation="MSTPFHSIEEAIKDIKKGKFIILVDDEDRENEGDLVMAAQHVTP
                     QAINFMAHYARGLICLALTPQRVEELHLPQQAVENTATFGTAFTVSIDARRDITTGIS
                     AADRATTIHVAIDPKSKPSDLARPGHIFPLKAQQGGVLKRAGQTEGSVDLARLAGLIP
                     AGVICEIMNQDGTMARVPELTKVAKARKIKMVTIKALIEYRMQRETFVRRMASARLPT
                     MFGDFEAVAFENEIDGITHIALVKGCVEGGDPTLVRVHSGCLTADALGSLRCDCRDQL
                     HKAMEVIQKEGRGVLLYLNQEGRGIGLLNKIRAYGLQDQGSDTVEANLQLGFKADLRD
                     YGVGAQILVNLGLHQIKLITNNPRKIVGIEGYGLQVVERVPIEIQPRAANMKYLRTKK
                     KKMGHILKKV"
     gene            2417..2962
                     /gene="ribH"
                     /locus_tag="VLL06_01425"
     CDS             2417..2962
                     /gene="ribH"
                     /locus_tag="VLL06_01425"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HSS29656.1"
                     /translation="MKLRKGSHDATGLRFGIVVAKFNKFVTSKLLSSCVEGLTKHGVK
                     ADDIEVVRVPGAFELPLVARTMARSKQFNAVICLGAVIRGDTPHFDYISAETSRGIGQ
                     AALDADVPIIFGVLTTHTVAQAIERAESAKFNRGGDAAKSAIEMATVMRLLREGEETP
                     SNVVIPRVRSAKGRAPRKRKK"
     gene            2964..3422
                     /gene="nusB"
                     /locus_tag="VLL06_01430"
     CDS             2964..3422
                     /gene="nusB"
                     /locus_tag="VLL06_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HSS29657.1"
                     /translation="MGSRHLARERALQILFQYDIHGKPGLWLDVFWKENEAPDEVKAF
                     AERLVAGVLENKKELNALIEKYATNWKISRMPIVDRNILRAGTYELLWMDDVPAKVTV
                     NEAIELAKSFGDDDASKFVNGILDKVLATEPRLAGKRKDALGVRDKESGV"
     gene            3427..4755
                     /gene="purB"
                     /locus_tag="VLL06_01435"
     CDS             3427..4755
                     /gene="purB"
                     /locus_tag="VLL06_01435"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HSS29658.1"
                     /translation="MIERYTRPQMKAIWELKRKYEIWLAVELAACAAFERAGQVPRGT
                     AARIGKKATIDVKRIARIEKVTKHDVIAFLESLVESVGPEHRFLHMGLTSSDIVDTSL
                     AVQMTEALDLILDDLDELIAVLKRQALKYKNQVMVGRSHGIHGEPISFGLKLAIWYEE
                     ACRHRERLMHVRREIAVGKLSGAMGTFAHQGPDVEAYVCAKLGLTPDPVSNQIVQRDR
                     HAAYASALALLAASIEKFATEIRHLQRTEVLEAEEYFSEGQKGSSAMPHKRNPIASEN
                     LCGLARVVRGNSLAAMENVALWHERDISHSSVERIIMPDSTILIDYMLARMTDVIKHL
                     VVYPANMKRNLNLTGGLVYSQRLLLRLIEKGAQRKESYEAVQRNAMASWQGAGDLQEL
                     VRRDPFVTKHLTAAEIRSCFDPKYYLRHLDKIFRRVFGPGGHKSGKGRKR"
     gene            4752..5549
                     /locus_tag="VLL06_01440"
     CDS             4752..5549
                     /locus_tag="VLL06_01440"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HSS29659.1"
                     /translation="MTFSISVLMRLIVLIIVGLATVPAVEAADRDKLLSEPGVYGTFA
                     AFSLDADWAKQDQSMRIAQLATLKGVVEQHREKLAIDTYLLRGLSDRADVLFRIHATE
                     PRETQQFLLDLQGSPFGKHLNTAGMMHGLTKKANYVPGMPDNLKAELKTPSEPGLKPY
                     VIVIPIRKDAEWWSLSQEQRAAMMQEHTEASLPYTKTVKRKLYHSTGLDDLDFITYFE
                     TSRLEDFQSLILALEKVKEFRHNRRFGHPTLLGTVRSLEEIIEVLAQ"
     gene            5575..6279
                     /gene="purC"
                     /locus_tag="VLL06_01445"
     CDS             5575..6279
                     /gene="purC"
                     /locus_tag="VLL06_01445"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HSS29660.1"
                     /translation="MTAGTLLYEGKAKKVFATDKPDQVVQYFKDDATAFNAQKRGTIV
                     EKGVVNNKVSERLFRLLDQAGVPTHFVERLSDRELLTKKVTIVPVEVVVRNIVAGSLA
                     KRLGLKEGDPIEPAIVEWYYKNDGLGDPLMADEHIRLHKLATPEQLGEMKRLALQVNR
                     VLRPFFHERQMILVDFKLEFGVYNGTLILADEISPDTCRFWDQTTKESMDKDRFRKDL
                     GKIEEAYREVLKRVCG"
     gene            6449..6673
                     /locus_tag="VLL06_01450"
     CDS             6449..6673
                     /locus_tag="VLL06_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29661.1"
                     /translation="MKGFRRLFMMYGLVASILAVMSVVGLLTYRMGESPWRWPLLAVI
                     VAGFGTIAGIFWIRRYVDRQTKASEQGSKQ"
     gene            6670..6909
                     /gene="purS"
                     /locus_tag="VLL06_01455"
     CDS             6670..6909
                     /gene="purS"
                     /locus_tag="VLL06_01455"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HSS29662.1"
                     /translation="MKAKIHVTLKQGILDPQGKAIEHALDSLGFKNAANVRVGKYMEV
                     DVNETDKAKAEAQVKQMCEKLLANTIVEEYRYELQ"
     gene            7093..7815
                     /gene="purQ"
                     /locus_tag="VLL06_01460"
     CDS             7093..7815
                     /gene="purQ"
                     /locus_tag="VLL06_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HSS29663.1"
                     /translation="MKIGVVVFPGSNCDHDCQHVFKDVLGQYVEMIWHKETLLAGLDA
                     IVLPGGFSYGDYLRTGAIARFSPVMGAVKEFAKKGGMVLGICNGFQILLEAGLLPGAM
                     LRNRSLHFICKDVHVKVENAATAFTGSCESGQVLKIPIAHADGNYYTDPVTLAAMQAN
                     AQIVLRYCTPEGKEIPEANPNGSLDNIAGIINAEGNVLGMMPHPERCTEDVLGNEDGR
                     LIFLSMLAGLKHATVKSNLVKV"
     gene            7880..8209
                     /locus_tag="VLL06_01465"
     CDS             7880..8209
                     /locus_tag="VLL06_01465"
                     /inference="COORDINATES: protein motif:HMM:NF015004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HSS29664.1"
                     /translation="MRIWDISPKKLCRNHLLGEHRELHAIWSVIVNGKKGYARHPETL
                     RWRGKLKALYGRHEALVSEMVDRGYQHRSPLAKREATGSGRQTTYVDRPRKQIVMLQN
                     KHCQCDV"
     gene            8222..>8923
                     /locus_tag="VLL06_01470"
     CDS             8222..>8923
                     /locus_tag="VLL06_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HSS29665.1"
                     /translation="MIVPAPVITKDLIAQHNLTDDEYKKIVEILGREPNITELGMFSV
                     MWSEHCSYKSSRVHLKKLPTTGPRVVQGPGENAGAVDIGDGLCAVFKMESHNHPSFIE
                     PYQGAATGVGGILRDIFTMGARPIALLNALRFGGLETAKNRHLMKGVVAGIAGYGNCM
                     GVPTVGGEIGFNDIYSQNPLVNVFCLGIAHKDKIFLGTAAGVGNPVIYFGSKTGRDGI
                     HGATMASDSFDDQSEQ"
BASE COUNT         1973 a         2136 c         2915 g         1899 t
ORIGIN      
        1 gctgggtgag ttggcggttg ccgcgtgggg atggttgggt gtccatagtt atgccgagtt
       61 gctgcttgtc gccatcttcc tcggtatcgg aggggcgagc ttcgcggtga ccttgccgct
      121 ggcgggccgc acctatccag cggcgcatca gggattggta ctgggaatcg ttgcgtcggg
      181 caatgtcggc acggtgttgg ttctcttttt tgcaccacgc attgcggcga ccggcggatg
      241 gcacgatgtg tttggctgga tgatgctgcc gattgtcctt gcgtttacgg tctttgtact
      301 cctggtgcgt gacgatcggc agagccggcg agctgaacgt gacgcacagt ggtggcacag
      361 tgtggcggtg atgatccgtc atccgaaagc ctattggctc tgcggactct atgctgtgac
      421 gttcggcgga tttgtcggat tctgcagtat tcttcccctg ttctttcacg agcagtacca
      481 ggtgaacggg atcgtcgcgg gctcgatcgt cgccttgtgt ggattggtgg gcagtcttat
      541 acgtccgttt gggggttacg cggcggaccg gatcggcggt ctgcgcacgc tcatactgtc
      601 attgcctctc atcgtaggga tcatcgcggt gctcggcgat gtcaccggcg tcaccgccgc
      661 agtcgtattg atggtggtgg cggtggggac tatgggggtc ggcaacgggg tcattttaca
      721 attggcctcg gagtggttcc caaaacaaat cggcctcgcc tcgggtattg tgggagcagc
      781 cggtggggtc ggcggttttc tcttgcccct ctggctgggg acgctgaaag aggtgaccgg
      841 ttcctatcga accggattat ggatgttcgc ggtcgcgaca ggcgttgcct ggggaacggt
      901 gctggttgca gaacgacgag cacgccgact catcaagccc atggccgtgg caggtcgtgt
      961 ttccattgac cgtgacggga ggtaaaaaac aatgttacat cttcgaatgt gaagtgataa
     1021 attgatcgtc ccttgaacct tcacaattta aacgttcaac gttggaatta tgtccactcc
     1081 ttttcatagc atcgaagaag ccatcaagga catcaagaag gggaaattca ttatcctcgt
     1141 cgatgacgaa gatcgcgaga atgaaggcga cctcgtcatg gcggctcaac acgtgacgcc
     1201 ccaggccatc aattttatgg cccactatgc ccgtgggttg atttgtctgg cgttgacgcc
     1261 ccagcgagtg gaagagctcc accttccgca gcaagcggtg gagaataccg ccacgttcgg
     1321 tacggcgttc actgtatcga tcgatgcgcg gcgtgacatt acgacgggta tttctgcggc
     1381 ggatcgggcg acgacgattc atgtggcgat cgatccgaaa tccaagccga gcgacctggc
     1441 gcggcccggc catattttcc ccctgaaggc gcagcagggc ggtgtgttga aaagggctgg
     1501 gcaaacggaa gggtctgtcg atctggcacg gctggccggg ctcattcccg ccggcgtgat
     1561 ctgcgaaatc atgaaccagg acggcactat ggcgcgcgtg ccggagctga cgaaggttgc
     1621 caaagcgcgc aagatcaaga tggtcacgat caaggcgctc atcgaatacc gtatgcagcg
     1681 cgagactttt gtgcggcgga tggccagcgc gcggctgccg accatgttcg gtgacttcga
     1741 ggcggtggcg ttcgaaaacg aaatcgacgg catcacgcac atcgctctgg tgaaagggtg
     1801 cgtggaggga ggcgatccca ccttggtgcg ggtgcactcg ggctgtttga ccgccgatgc
     1861 cctaggatca ttgcggtgcg actgccgcga ccaactccat aaggcgatgg aggtgattca
     1921 gaaggaaggt cgtggggtcc tgctctatct taatcaagaa gggcgcggaa tcggcttgct
     1981 caacaagatt cgggcctatg ggttgcaaga tcaggggagc gatactgttg aggcgaattt
     2041 gcagttggga tttaaggccg acctgcgcga ttacggcgtg ggggcgcaga ttctcgtcaa
     2101 tctcgggttg caccagatca aactcatcac caacaatccg cgcaagatcg tcggtatcga
     2161 aggctatggg ttgcaagtgg tcgaacgtgt gccgatcgag attcagccgc gtgcggcgaa
     2221 tatgaagtat ctgcgaacga aaaagaagaa gatggggcat atcctaaaaa aggtgtgaga
     2281 agggaatggc cagcccgccc ttcttgctcg cagaacgcgc acgataagaa tgtgctcgtt
     2341 cgatgcgcgc agttgaaggg caagctggcc gttcccttgt tcgtggcgaa cgtgctggag
     2401 gaaatgaaac aacaagatga aactgcggaa gggttctcat gatgcgacgg gattgcggtt
     2461 tgggattgtt gttgcgaagt tcaataagtt cgtcacgagt aagctgctga gttcctgcgt
     2521 tgaaggactc accaaacacg gggtcaaggc cgacgacatt gaggtggtgc gggtgccggg
     2581 ggcgtttgag cttcctctgg tcgctcggac gatggcacgg tccaagcagt tcaatgcggt
     2641 gatttgtctc ggcgcggtga ttcgcggtga cacgccgcat ttcgattata tcagcgccga
     2701 gaccagtcgt gggattgggc aggcggcgct ggatgccgat gtgccgatca tcttcggcgt
     2761 cctgacgacg cacacggttg cgcaggcgat tgagcgggct gaatcagcca aattcaatcg
     2821 tggcggggat gcagccaagt cagcgattga aatggcgacg gtgatgcgtc tgttacgaga
     2881 gggtgaggaa actccatcga acgttgtaat cccgcgagta cggagcgcga aagggcgggc
     2941 gccgcggaag cgcaagaagt agcatgggat cacgccatct agctcgggaa cgcgcgctcc
     3001 agattctgtt tcagtacgat atccacggca agccgggctt gtggttggat gtgttttgga
     3061 aggagaacga ggccccggat gaggtgaagg cctttgccga acgacttgtg gccggagtgt
     3121 tggaaaacaa aaaagagctg aatgccctca ttgagaagta cgccaccaac tggaaaatta
     3181 gtcgtatgcc gattgtggac cgcaacatcc tgagagccgg cacctatgaa ctgctgtgga
     3241 tggatgacgt gccggccaag gtgacggtga atgaagcgat agaattggcg aagagtttcg
     3301 gggatgacga cgcctcgaaa ttcgtgaatg gcattttgga taaggtgctg gcgactgagc
     3361 cgcggctggc aggaaagcgg aaagacgcgt taggagtgag ggataaggag tcaggggtgt
     3421 gacgagatga tcgagcgcta tacccgtccc cagatgaagg ccatctggga attgaaacgt
     3481 aagtacgaga tctggcttgc ggtcgaactg gccgcctgcg cggcattcga gcgagccgga
     3541 caggttccac gcggcactgc ggcccgaatc gggaagaagg cgacgatcga tgtgaaacgg
     3601 atcgctagga tcgaaaaggt gaccaagcac gatgtgatcg cctttctcga atcgctcgtg
     3661 gagtcggtcg gacccgagca tcggtttctc catatgggac tgacctcctc ggatattgtg
     3721 gacacgtcat tggccgtgca gatgaccgag gcgcttgacc tcattctcga cgatcttgac
     3781 gaactgatcg ctgtcttgaa gcggcaggca ctgaaataca agaatcaggt catggttggc
     3841 cgctcgcatg gcattcacgg cgaaccgatc tcgttcggtt tgaagctggc catttggtat
     3901 gaagaagcgt gccggcaccg ggagcggctg atgcacgtgc ggcgggagat cgcggtcggc
     3961 aagctttccg gtgcaatggg cacgttcgcg catcaagggc ctgatgtgga agcgtatgtc
     4021 tgtgccaagc tggggctcac accggaccct gtctccaatc aaatcgtgca gcgcgatcgt
     4081 catgcagcct atgcctcggc attggcgcta ttggctgcca gtattgaaaa gtttgccacc
     4141 gagattcgcc atctacaacg gaccgaggtg cttgaagcgg aagaatattt ctccgaagga
     4201 cagaaaggat cctcggcgat gccgcacaag cggaacccga tcgcctcgga gaacctttgc
     4261 ggactggcac gcgtggtgcg gggcaatagc ctcgcagcaa tggagaatgt cgcgctgtgg
     4321 catgagcgcg atatcagcca ttcctccgtc gagcgcataa tcatgccgga cagcaccatc
     4381 ctgatcgatt atatgctggc gcggatgacc gacgtcatca agcatctggt ggtctacccg
     4441 gccaatatga aacgcaacct caatctcacc ggggggctgg tctactcgca acggttgctg
     4501 ctgcggctca tcgagaaggg cgcgcagcgc aaggaatcgt atgaagccgt ccagcgcaat
     4561 gccatggcct cctggcaagg ggcaggggac ctacaagaat tagtccgcag ggaccccttc
     4621 gtgacgaaac atctcacggc agcggagatt cgatcatgtt tcgatccgaa gtattatctg
     4681 cgccatctcg acaagatttt ccgacgggtg ttcggaccgg gagggcataa atcagggaag
     4741 ggcaggaaac gatgacattc tccatctctg tgctcatgcg tcttattgtg ctgatcattg
     4801 tcggcctggc gacggtgccg gccgtcgagg cagccgatcg cgacaagctg ctcagtgaac
     4861 cgggcgtcta tggcaccttt gccgccttca gtctcgatgc agactgggcg aagcaggacc
     4921 agtcgatgcg catcgcgcag ttagccacgc tgaagggggt ggttgagcag catcgagaga
     4981 agttggcgat cgatacctat ctccttcgcg gactctccga tcgcgctgat gtgctgtttc
     5041 gcattcatgc gaccgagcca cgcgaaaccc aacagtttct ccttgatctt cagggtagtc
     5101 cctttgggaa gcatctcaat actgccggca tgatgcatgg cctgacgaag aaggcgaact
     5161 atgtgccagg catgccggac aatctcaagg cagagctgaa gactcctagc gaaccaggcc
     5221 tcaagcccta tgtgatcgtg atcccgatcc ggaaagatgc agagtggtgg tccctttcac
     5281 aagagcagcg cgcagcgatg atgcaggaac atacggaagc gtcgttgccc tacaccaaga
     5341 cagtcaaacg gaagctgtac cattcgaccg gtcttgacga tctggatttc attacctatt
     5401 ttgaaacgtc acggctggaa gactttcaga gtttgattct ggcattggag aaagtgaaag
     5461 agttccggca taaccggcgg ttcggccatc cgaccttgct aggaacggtc agatcactag
     5521 aagaaatcat cgaagtgttg gcgcagtgaa cgtgaaccca gttgaggcag agctatgacg
     5581 gcgggcacgc tcctctacga agggaaagcc aagaaagtct ttgcgaccga caagccggat
     5641 caggtcgtcc agtacttcaa agacgatgcg acggcgttca acgcgcaaaa acgtgggacc
     5701 atcgtcgaga aaggggtcgt caacaataaa gtgtccgaac ggctctttcg attgttggat
     5761 caggccggtg tgccgacgca ttttgtcgaa cggctgagcg accgggaatt gctcacgaag
     5821 aaggtcacga tcgtaccggt cgaagtcgtg gtccgcaata ttgttgcagg cagtttggcg
     5881 aagcggctgg gattgaaaga gggtgatcct atcgagccgg ccatcgtgga gtggtactac
     5941 aagaacgatg ggttgggtga tccgctcatg gccgatgagc acattcggtt gcacaagctg
     6001 gcgacgccgg aacaactggg ggagatgaag cggctggcgc tacaggtgaa tagggtcctc
     6061 cgtccgttct ttcacgaacg tcagatgatc ctcgtggatt tcaaattgga gtttggagtc
     6121 tacaacggca cgctgatcct agccgacgag atttcgccgg acacctgccg cttttgggac
     6181 cagacgacga aggagtcgat ggataaggat cggtttcgga aggatttggg gaagattgag
     6241 gaggcgtatc gggaggtgtt gaagagggtg tgcggatgag gggacgaagg ggatgctggg
     6301 gacggtgggg ccgtcctcct tgctcgcgca acgcgcggtc gaagacgacc ctcgttggac
     6361 gcgcgcagtt gaggacaacc ccaccatccc cttctcagat cactccaaat cctttggtga
     6421 tgggagagtt aggagaggga gagggaaagt gaaggggttt cggcggttgt tcatgatgta
     6481 tgggttggtg gcctccattt tggcggtcat gtcggtagtt ggactattga cctatcggat
     6541 gggggagtca ccttggcgct ggccgctctt ggcggtgatc gtggctgggt tcgggacgat
     6601 tgcggggatt ttctggatca ggagatatgt ggacagacag accaaagcat ccgagcaggg
     6661 gagtaaacag tgaaggctaa aatccatgtc acgttgaaac agggcatatt ggatcctcag
     6721 gggaaagcga ttgagcatgc actcgattcg ctggggttca agaatgcggc caatgtgcgg
     6781 gttgggaagt acatggaagt cgatgtgaat gaaacggaca aggcgaaggc tgaggctcag
     6841 gtgaagcaga tgtgcgaaaa gctattggcc aacacaattg tcgaagagta tcggtatgag
     6901 ttgcagtgag atggcaagaa tagggaagcg gccggcgatc gttcttgttc gcagaacgcg
     6961 cacgatgaag ggtgctcgct tgggtcaagg gcgcgtcttt gcgcgcccgg ggtgggtggg
     7021 tgagaatatt gcgcgcagtg aacgacggcc tagccacttc cctgatgagt tgagaaacgc
     7081 aaggaagagt atatgaagat cggtgttgtt gtttttccag gcagtaattg tgatcacgac
     7141 tgccagcatg tcttcaagga tgtgctaggg cagtatgtgg aaatgatctg gcacaaggag
     7201 accttgctgg caggtttgga tgcgattgtc ctgccgggtg ggttctccta tggcgactac
     7261 ttgcggaccg gcgccatcgc gcggttctcg ccggtcatgg gggcggtgaa ggagtttgcg
     7321 aagaagggtg ggatggtgct cggcatctgc aatggatttc agattctcct ggaagcggga
     7381 ctgttgcccg gtgcgatgtt gcgcaaccgc tccttgcatt tcatctgcaa ggatgtgcac
     7441 gtgaaggttg agaatgcggc gacagcgttc acaggaagct gtgagtcggg gcaagtattg
     7501 aagattccga tcgcccatgc agacgggaac tactacaccg atccggtgac gcttgcggcg
     7561 atgcaggcga atgcgcaaat tgtgctgcgc tattgcactc cggagggcaa ggagataccg
     7621 gaggcgaacc cgaacggctc gctggacaac attgcgggaa ttatcaacgc agaaggcaat
     7681 gtgctcggca tgatgccgca tccggaacgc tgcacggaag atgtgctagg caatgaggat
     7741 ggccgattga tttttctgtc gatgttggca gggctcaaac atgccacagt gaaatcgaat
     7801 ttggtcaagg tgtgaagcaa atctcgcctg ctcttaacga ggttctcacc gcgcagcatg
     7861 atctgaccga cgaggagtaa tgaggatttg ggatatttcg ccgaagaaac tctgtcggaa
     7921 tcatttgttg ggagagcatc gggagttgca cgcgatatgg tcggtgatcg tgaatggcaa
     7981 gaagggctat gccaggcatc cggagacgtt gcgctggagg gggaaactca aagcgcttta
     8041 tggcaggcat gaggcgctgg tgagtgagat ggttgaccga ggataccagc atcgcagccc
     8101 gctggcgaag agagaggcga cgggcagcgg aaggcagacg acctacgtgg atagaccgag
     8161 aaagcagatt gtgatgttgc agaacaaaca ttgtcagtgt gatgtgtaac gagaggctgt
     8221 ggtgatcgtg ccagcaccgg tgataacgaa agatctcatc gcgcagcata atctgacgga
     8281 cgatgagtac aagaagatcg tcgagatact cgggcgggaa ccgaatatca ctgagttggg
     8341 catgttttcg gtgatgtggt ccgagcattg ttcctacaag agttcgcgcg tgcacctcaa
     8401 aaaattgccg acgacggggc cgcgcgtggt ccaggggccg ggggaaaatg ccggtgcggt
     8461 ggatatcggc gacggactct gcgcggtctt caagatggag tctcacaacc atccgtcgtt
     8521 tatcgagccc tatcaaggtg cggcgaccgg agtgggcggg attctgcgcg acatctttac
     8581 gatgggtgcg cggccgattg ccttgctcaa tgcgctgcgg ttcggtgggc tggagacggc
     8641 aaagaatcgc cacctcatga agggcgtcgt ggccggcatc gcgggctacg gcaactgcat
     8701 gggagtgccg acggtcggag gcgaaatcgg ctttaacgat atttattcac agaatccgct
     8761 cgtgaacgtc ttttgtctcg gcatcgcgca caaggacaag attttccttg gaacagcggc
     8821 aggggtcggc aatccggtga tctatttcgg ttcgaaaacc ggacgcgacg ggattcatgg
     8881 cgcgacgatg gcctccgatt ctttcgacga ccagtcggaa cag
//
LOCUS       DATIPF010000055         8007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_980523, whole genome shotgun sequence.
ACCESSION   DATIPF010000055 DATIPF010000000
VERSION     DATIPF010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8007
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980523"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VLL06_01475"
     CDS             <1..928
                     /locus_tag="VLL06_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HSS29666.1"
                     /translation="QIVGGVAQAYFDLRQFDMQLEIAKRTLLAWEESVRIGQARLRQG
                     VINRLDVDQFEAERENAAARIAELKRQMIQKENELSVLLGRNPSQIARGRSLTEQMMP
                     PMVPSGLPSELLQRRPDVVQAEQQLVAVTARIGVAKAERFPKLSLTGLLGVASPHLTD
                     FVSPSTSFGVLAPVLTGPLLNAQTLGFQQRAVEAQTKQAVAQYEQTILVAFREVEDAL
                     VGVSTAREQAAAQERQVNALRSALHLANLRYKGGLANYLDVLIAQRNLFEAELSLAAT
                     RRLHLVSVVQLYKALGGGWSPEKDRLKDGSKG"
     gene            1119..1718
                     /locus_tag="VLL06_01480"
     CDS             1119..1718
                     /locus_tag="VLL06_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HSS29667.1"
                     /translation="MEKPADTQYQIHDLLRRRWSPRAFDDRSIEPEKLRSLFEAARWA
                     PSSNNEQPWRFLVATKENTTEYDRLFGCLVEGNQKWAHRAPALLLSVAKLQFEDDSPN
                     RHALHDTGMAVENLVLQATALGLVVHQMAGFRIDQARTDCKIPADYEPVAIIAIGYPG
                     DPAQLSDRLRAREAQPRVRKPLTEFVFSATWSQPSPLFR"
     gene            1737..2468
                     /locus_tag="VLL06_01485"
     CDS             1737..2468
                     /locus_tag="VLL06_01485"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HSS29668.1"
                     /translation="MASLSGKVAIVTGSSRGIGRAIAERLAEDGAIVVVNFRTSVQQA
                     RQVVTGIQAKGGKAVAVQSDMSQVAEARRLVIETVKQFGRLDILVNNAGRFMPQPLME
                     TTEADFDDIVALNAKGPYFAMQEAARVLKDGGRIVNISTDGTHLNFPGATAYLGSKGA
                     LEQYTKGLAQELAPHGITVNTVSPGFTETGMMTDQFRQIGIQMSPLKRLGAPKDIADV
                     VAFVVSPEARWLTGQNIHAGGGIVM"
     gene            2479..2874
                     /locus_tag="VLL06_01490"
     CDS             2479..2874
                     /locus_tag="VLL06_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSS29669.1"
                     /translation="MGRQNISTGGPWESKLGYSRAVRVGTSIQVSGSTAMTPSGLVGK
                     GDPYAQTVQTLKTIEVALQQAGASLADVVRTRMYLVNIDQWQDVGRAHGEVFGMIRPA
                     TTMVEVKRLIDPDMLVEIEADAILTHEKS"
     gene            2898..3551
                     /locus_tag="VLL06_01495"
     CDS             2898..3551
                     /locus_tag="VLL06_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HSS29670.1"
                     /translation="MDRYTEETRHLSKTDPVMRRLIREVGPYTLIPRSKRSPFESLAR
                     AIAYQQLHEKAAECILRRFVALCPARRFPQPDDLLALNEQVIRSAGFSRAKVAALRDL
                     ATKTLEGTVPTGTIIRALDDEAIIERLIAVRGVGRWTVEMLLIFQLGRPDVLPVDDFG
                     VRNGFRIAYGRGSMPLPKDVRQYGERWKPYRTAAAWYLWRAADRERESKKVSGVNAS"
     gene            3593..4444
                     /locus_tag="VLL06_01500"
     CDS             3593..4444
                     /locus_tag="VLL06_01500"
                     /inference="COORDINATES: protein motif:HMM:NF025948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSS29671.1"
                     /translation="MSQVTFLSMRLALSVIPAGSWPPASFEEVGSFSAVTQQPTDHGS
                     IQQAYQALLACPVGAIGTEHNDKIHMRDAMATFPLHLEGGVYYCGFNSDKSFGANSFF
                     VEHPDGNWLVDSPRYLKHLVEAFEHKGGIAYIFLTHKDDVADAEKYAAHFGAKRLIHR
                     ADLEAAPDSEWIIEGTDSKQVMPQFLVIPVPGHTAGSMALLYKNRFLFTGDHLWWDSA
                     QKRLGAPTRLVWRKHVLVDSIKKLLDHPFEWVLAGHGDRVRLPAEDMRAQLQALVGRR
                     QPGHVMS"
     gene            4447..6081
                     /locus_tag="VLL06_01505"
     CDS             4447..6081
                     /locus_tag="VLL06_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29672.1"
                     /translation="MFTRLAQWIDVNILSLGREMRLSYLPPLMVYMAYGISGLTGIVG
                     TFFVKDYLGLSASFLAALGFWAGIPWALKMPIGHLVDLLWRWKGWLVGLGAGLLAASL
                     SIMAALIGDREVMTSILPAEVWYVISTLLGPIGYVVQDAVADAMTVEAVPRVDGHGQL
                     FDPPTLKLMHTTMQTLGRVAIIGGGILVAIINVYVFTGVEGLPQIQIIGLYQQVYLMA
                     LIIPFVSVLGVGMAWMLRQRHKRRLLQEGFSLEQAGQMVNARDDVQEPTKPNWWILAG
                     SLAFVLFTLTVGFGDFSYSEEIVFAGSMTIVVFLMARLVRELEPDKRFTLVGTAVVVF
                     IFRAIPGTGPGTTWWMIDQLKFDQHFFSILSLIGSTLTLAGMFVFRRFMAERSIAYVV
                     GFLTVIGTILTLPIVSMFYGLHEWTARITGGIVDARFIALIDTALESPLGQISMIPML
                     AWIANTAPANLKATFFAVMASFTNLALSLSQLGTKYLNDLYVVTREVHDQVSGAIQTP
                     ADYSHLGSLLIAQLVLGLALPFAAIVFAKVTRFKSA"
     gene            6347..7291
                     /locus_tag="VLL06_01510"
     CDS             6347..7291
                     /locus_tag="VLL06_01510"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSS29673.1"
                     /translation="MVWLCQIMTVVLISMAWLCSVDHKAAFATEAAPTKPESVQVPPA
                     SASTPTRPKPPAKSQSAANGSGASKPGGLPQPPAQVMGKDGAPMVLIPAGEFTMGSDK
                     GDDDEEPIHRLFLDSFYMDKFEVTNGRFAKFVEAIQSEPPWGFADQETPVVHADQPVR
                     WVNWMDAWGYCLWAGKRLPTEAEWEKAARGTDGRLYPWGNEPPTPLHAVFGLKEGAET
                     VSPIGNRTQGRSPYGAHDLAGNLYEWVADWYDDAFYTTNPTVNPRGPLQGTTKVQRGG
                     SYINTPYRLRSSFRTKADPTEHEPNVGFRCAQDAPSLP"
     gene            complement(7389..>8007)
                     /locus_tag="VLL06_01515"
     CDS             complement(7389..>8007)
                     /locus_tag="VLL06_01515"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSS29674.1"
                     /translation="FFRPNYLGNLVTNWIPSLDGVEAKLKKGAKVADVGCGFGASTII
                     MAKAYPKSTFIGFDYHKPSILTARKRAKDVGLKNVTFTTAKSTDYPGKGYDFIAHFDC
                     LHDMGDPVGAAKHVKKTIAPDGTWMIVEPFAHDRVEDNLNPVGRVFYAASTMICVPAS
                     LASNGPALGAQAGEARLRDVVLKGGLRRFRRATHTPFNLVLEARP"
BASE COUNT         1652 a         2205 c         2426 g         1724 t
ORIGIN      
        1 gcaaatcgtg ggcggagtgg cgcaggccta tttcgatctt cggcaattcg acatgcagtt
       61 ggagatcgcg aaacgcacct tgctggcctg ggaggaatcg gtgagaatcg ggcaagcacg
      121 gctccggcag ggcgtgatca acaggctgga tgtcgaccag ttcgaagcgg agcgggaaaa
      181 tgctgccgct cgcattgcgg agttgaagcg tcagatgatc caaaaagaaa acgagctcag
      241 cgtgctgctg gggaggaatc ccagtcaaat cgccaggggg cgatcactga cagaacaaat
      301 gatgccgcct atggtaccct ccggtcttcc ctcggaactg ctccaacgcc gtccggatgt
      361 tgtgcaggcg gagcaacaat tggtggcagt cactgccagg atcggggtgg cgaaggccga
      421 acgatttccc aaactgtcgc tcacgggact tctcggtgtg gcgagtccgc acttgacgga
      481 ttttgtgtcg ccgtcaacca gtttcggcgt gctcgctccg gtactgaccg gccccttgtt
      541 gaatgcccaa acgttagggt ttcaacagag ggccgtcgaa gcccaaacca aacaggctgt
      601 ggcgcagtac gaacaaacga ttctcgtcgc gttcagggag gttgaagacg cgctggtggg
      661 ggtcagcacg gcccgtgagc aggcggcagc ccaggaaagg caggtcaatg ccctgcggtc
      721 ggccttgcat ctggccaatc tgagatacaa aggcggtctg gcgaactacc tagatgtcct
      781 cattgcgcag cggaaccttt ttgaggcgga attgtcgctt gccgccaccc gtcgtctcca
      841 tctggtctcg gtggtccaac tctataaggc ccttggtgga ggctggtctc ccgagaagga
      901 ccgactcaag gacggcagca agggatagca tgcttcggaa acattgtggg catgttggat
      961 gccttaagaa gagcacatca cgaaggatgc tcaaaatgac cgtccagcaa ggccgcagcg
     1021 agtgaagacc cgaggcgtac cctctggggt acgttgaggg tctgaacgaa acgagaacgc
     1081 cgctggcggg cttgtttagc atcctgcgag agacgcacat ggaaaaacca gctgacacgc
     1141 agtaccagat ccatgacttg ttgcgccggc gttggagtcc acgcgccttt gacgatcggt
     1201 ctatcgagcc ggagaagttg cggagtcttt tcgaagcagc acggtgggcg ccttcttcga
     1261 ataacgaaca gccttggcgg ttcttggtcg ccacgaaaga gaatacgacg gagtatgatc
     1321 ggctgttcgg ctgcctggtc gaaggcaatc agaaatgggc gcatcgcgcg ccggcgctgc
     1381 tgctgtccgt cgcaaaactg caattcgaag atgactcgcc gaatcgtcac gcgctccatg
     1441 acacgggcat ggcggtggag aatctcgtcc tccaggccac agcgctcggg cttgttgtcc
     1501 atcagatggc aggctttcga atcgatcagg cgcgaacgga ctgtaagatc ccagcggact
     1561 atgagccggt ggccatcatt gcgatcggtt atccaggaga tcccgcccag ctgtcggacc
     1621 gcctgcgagc acgggaagct caaccaaggg tccgcaagcc tctgactgaa tttgtattct
     1681 cagctacgtg gtcgcaaccg tcccctcttt tccgatagga agggcagaga agacacatgg
     1741 cgtcactcag tggaaaagtt gcaatcgtca cggggtcatc cagaggaatc ggacgggcga
     1801 tcgcggagcg cttggccgaa gacggggcga tcgtggtggt gaatttcaga acaagtgtgc
     1861 agcaggcccg gcaagttgta acgggcattc aggccaaagg agggaaggcc gtcgctgtcc
     1921 aatccgacat gagccaggtc gccgaggcgc gccgtctcgt gatcgagacg gtgaagcaat
     1981 tcggccgact cgacatcctc gtcaacaatg ccggacggtt tatgccgcag ccgctcatgg
     2041 agaccactga ggcggacttc gatgacatcg tcgccttgaa cgccaagggt ccctacttcg
     2101 cgatgcagga agcggccagg gtactcaaag acgggggccg catcgtgaat atttccaccg
     2161 atggaaccca tttgaatttt cccggtgcga cggcttatct tggcagcaaa ggtgcgctgg
     2221 aacagtacac gaaaggattg gcccaggaac tcgctccaca tggcattacc gttaatacgg
     2281 tctcgccggg ttttaccgag acaggtatga tgacagatca gttccgccag atcggaatac
     2341 agatgtcgcc gttgaagcgg cttggtgcgc cgaaagacat cgccgatgtg gtcgcgttcg
     2401 tcgtcagtcc ggaggcccgg tggttgactg gccagaatat tcatgctggc ggtggcattg
     2461 tgatgtagga gggactagat gggacggcag aatatttcca ccggtggtcc ttgggagagc
     2521 aaactggggt actcgcgagc ggtacgagtc gggacctcaa tacaggtctc cggatcgacg
     2581 gccatgacgc cctctggtct ggtcggcaag ggggatccct atgcccagac agttcagacg
     2641 ctgaagacga ttgaagtggc gctccagcag gccggagcct cgcttgccga tgtggtccgg
     2701 acgcgtatgt atctggtgaa catcgatcaa tggcaggacg tcggacgcgc ccatggtgaa
     2761 gtattcggga tgatcaggcc tgcgacgacg atggtggagg tcaagcggct gatcgatcct
     2821 gacatgttgg tcgagatcga agccgatgcg atcctcaccc atgaaaaatc ttagcaagac
     2881 ggcgcgcgga ctctcatatg gatcgataca ccgaagaaac caggcatctg tccaagacgg
     2941 acccggtcat gcgccggttg atccgtgagg tgggtcccta taccctgatt cctcgatcga
     3001 agcggtcgcc gtttgaatcg ttagcgcgag cgattgccta tcaacaactg cacgagaaag
     3061 cggcggagtg tattttgcgc cggtttgtgg ccttgtgtcc ggcaagacgg ttccctcaac
     3121 cggacgatct gctcgcgttg aacgagcagg tgattcggag cgccggcttt tcacgagcca
     3181 aggttgcagc gctgcgcgat ttggcgacca agacactcga aggtactgtg ccgacaggaa
     3241 ccatcattcg agcactcgac gacgaggcga ttattgagcg gctcattgcg gtccgtgggg
     3301 ttggccggtg gacggttgaa atgctattaa tctttcaact ggggcgaccg gatgtcctgc
     3361 cggtcgacga cttcggtgtg cgcaacgggt tccgcatcgc ctatggccgt ggctcgatgc
     3421 cgctgccgaa agatgtgcga caatatggag aacggtggaa gccctatcgc accgctgcag
     3481 cctggtatct ctggcgtgca gccgaccgtg agagggaatc caagaaggtt tcaggcgtca
     3541 acgcatcata gaggtcgtgc tatggcggat gagaaaaaac gactcgactc caatgtcaca
     3601 ggtaactttt ttgtcgatgc gacttgcatt atctgtgata cctgccggca gctggccccc
     3661 ggcaagcttt gaagaggtcg gtagtttttc cgcggtcacc caacagccga ccgaccatgg
     3721 gtccattcaa caggcctatc aagcactcct cgcttgcccg gttggcgcga tcggcacgga
     3781 gcacaatgac aagatccaca tgcgggacgc catggcgacc ttcccgctcc atctggaagg
     3841 cggcgtctac tactgtggct tcaactcgga taaatcattt ggtgcgaata gcttcttcgt
     3901 cgaacatccg gacggcaact ggcttgtcga ttcaccgcgc tatctcaaac atctcgtcga
     3961 agctttcgaa cataagggcg gcatcgcgta catctttctc acgcataaag acgatgtggc
     4021 tgatgcagag aagtatgctg cgcacttcgg cgcgaaacgg ctgattcatc gggcggacct
     4081 cgaagcggct ccggattcag aatggatcat tgagggaact gacagtaagc aggtcatgcc
     4141 gcagtttttg gtcattcctg tgccgggcca tacggcaggc agcatggctc ttctctacaa
     4201 gaacagattc ctcttcacgg gagatcatct ctggtgggac tcggcacaga agaggctcgg
     4261 agctccgacc cgactggtgt ggaggaagca tgtactagtg gactccatca agaagctcct
     4321 cgaccacccc ttcgaatggg tgctggcagg gcatggggat cgggttcggc tcccggctga
     4381 ggacatgcgc gcgcagctgc aggcgttggt cggacgtcgc cagcccggtc atgttatgtc
     4441 atagcgatgt tcacgcgcct cgctcagtgg atcgacgtca acattctttc ccttggccgt
     4501 gagatgcggc tctcctatct gccgcccctc atggtctaca tggcctacgg catctccggc
     4561 ctcaccggca ttgtggggac cttcttcgtc aaagactact tggggctctc cgcgtcattt
     4621 ctcgcggcgc tgggattttg ggccggtatt ccttgggcac tcaagatgcc catcggacat
     4681 cttgtcgatc tcttgtggcg ctggaaaggc tggctggttg gccttggagc tgggctgctg
     4741 gccgccagcc tcagtatcat ggcggcgctg atcggggacc gtgaagtgat gacctcgatc
     4801 cttcctgcgg aagtctggta cgtgatcagc accttactgg ggccgatcgg gtacgtggtg
     4861 caagacgcgg tagcggatgc gatgacagtt gaggcagtgc ctcgcgtcga cgggcatggg
     4921 cagttgttcg acccaccaac cctcaagctc atgcacacca ctatgcaaac tctcggtcgt
     4981 gtggcgatca ttggtggcgg cattctggtc gcgataatca acgtgtatgt cttcaccggg
     5041 gtcgaaggac ttccgcagat acagatcatc gggctctatc aacaggtcta tctcatggcg
     5101 ttgatcatcc catttgtctc agtgcttggt gtggggatgg cctggatgct gcgtcagagg
     5161 cacaagaggc gattgctcca agaaggattt tcccttgagc aagcggggca gatggtgaat
     5221 gcacgtgatg acgtacagga gccgacgaag cccaattggt ggattctagc cgggagcctt
     5281 gcctttgtcc tgttcacgct gacggtcgga ttcggcgact tctcgtatag cgaagagatt
     5341 gtgtttgccg gatcgatgac catcgttgtg ttcttgatgg cccggctggt tcgagagtta
     5401 gaacccgaca aacgattcac tctggttggg acagcggtcg tggtatttat cttccgagca
     5461 atcccaggga ctgggccagg aacaacgtgg tggatgattg accagctgaa gttcgatcaa
     5521 cacttttttt ccatcctctc gctgatcggc agcacgctca ccttggcggg catgttcgtc
     5581 tttcgccgct tcatggccga gcggtcgatt gcatatgtcg tggggtttct cacggtgatc
     5641 gggaccattt tgaccctccc gatcgtgagt atgttctatg gcttgcacga gtggacggcg
     5701 agaattacgg gagggattgt cgatgcacga ttcattgcgc tgatcgatac ggcgttagag
     5761 tcgccgctcg gtcagatctc gatgattccg atgctggcct ggatcgcgaa cacagcacca
     5821 gcgaacttga aagccacctt ctttgccgtc atggcctcat ttacgaatct cgccttgtca
     5881 ttgagtcagc tcggcaccaa atacttaaac gatctctatg tggtcacgcg agaggtgcac
     5941 gaccaggttt ccggtgccat ccaaacgccg gcggactata gtcaccttgg ctcgctcctg
     6001 attgcgcagt tagtcctggg cttggccctc cccttcgcag cgattgtgtt tgccaaagtc
     6061 actcgcttca aaagtgcgta gcaggctgtt gaacagttcc gcttgcctcg tgatcatggt
     6121 cagtggataa aagtccttcc caccttcttg ttctgtgtac tttcggtaag actacggtct
     6181 atgtctctct cggctgtatg ccttcgccta cgcatacctc ggttcggatg atccgacgag
     6241 agtccaaatc agtttttcac gctagatttg ttgtcgcctt tttcggtacc ctagacaggc
     6301 gaagatgtga ctttgatctt ctcctaatct tggaatgtgc gttgccatgg tgtggctctg
     6361 tcagatcatg acggttgtgc tgatcagcat ggcgtggctc tgctctgtcg accacaaggc
     6421 ggcctttgcg acggaagctg ctcccacgaa acccgaatct gtccaggtgc cgcctgcgtc
     6481 tgcgtcgaca ccgacgagac caaaacctcc cgcaaaatct cagtctgcag ccaatgggtc
     6541 tggtgcctcc aaaccaggtg gtctgccaca acctcctgcc caggtgatgg gaaaagatgg
     6601 cgcgcccatg gtcttgatcc cggccggcga gttcacgatg ggcagcgaca agggcgatga
     6661 cgacgaggag ccgattcacc gcctgtttct cgacagtttc tatatggata aattcgaggt
     6721 gaccaacggc cggtttgcca agttcgtcga ggccattcag agcgaaccgc cctgggggtt
     6781 cgcggaccaa gagacgcccg tcgtccatgc ggatcagccg gtgcgctggg tcaactggat
     6841 ggacgcgtgg ggctattgtc tctgggctgg gaagcgcttg ccgaccgaag cggaatggga
     6901 gaaagccgcg cggggcaccg atggccggct ctatccctgg gggaacgagc cgccgactcc
     6961 gctgcatgcg gtcttcgggc tgaaggaggg ggccgagacg gtctcgccga tcggcaatcg
     7021 cacccagggg cgcagcccct atggggccca cgatctggcg ggcaatctat acgaatgggt
     7081 ggcggactgg tacgacgacg cgttctacac gacgaatccc accgtcaatc cgcggggccc
     7141 gctccaggga acgacgaagg tgcagcgggg cggctcctac atcaacacgc cctatcggtt
     7201 gcggtcgtcc ttccgcacca aggccgatcc gaccgaacac gaacccaacg tcgggtttcg
     7261 ctgtgcccaa gacgcgccat ccctgccata gccgggctag cctccgctag catttcaggt
     7321 tacccgcgat gaaggataga ctactggaaa gaccaggtac atccacagtc gagaactctc
     7381 gtatcaagtc acggcctggc ttccaatacc aaattgaatg gcgtgtgcgt cgcgcggcgg
     7441 aatcgtctga gcccaccttt caggaccacg tctctcagcc gggcctctcc tgcctgagcg
     7501 ccgagggcag ggccattgga agccagtgaa gctggtacgc agatcatcgt cgacgcagcg
     7561 tagaagactc taccgaccgg attcagatta tcttccacgc ggtcatgtgc gaacggctcc
     7621 acgatcatcc aggtcccgtc tggtgcgatt gtcttcttca cgtgtttcgc agcgccgaca
     7681 ggatccccca tgtcatgcag acagtcgaag tgggcgatga agtcatatcc cttgcccgga
     7741 taatctgtgg acttggctgt tgtgaaggtc acgttcttca agcctacgtc cttggcccgt
     7801 ttgcgcgccg tcaggatcga aggcttatgg tagtcgaagc cgatgaaggt ggatttggga
     7861 taggctttcg ccataatgat ggtggaggcc ccgaacccgc agccgacatc cgcgactttc
     7921 gcccctttct tgagctttgc ctccacgccg tcgagagacg ggatccaatt cgtcacgagg
     7981 ttgcccaggt agttcggacg aaagaac
//
LOCUS       DATIPF010000056         4580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1158002, whole genome shotgun sequence.
ACCESSION   DATIPF010000056 DATIPF010000000
VERSION     DATIPF010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158002"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /locus_tag="VLL06_01520"
     CDS             complement(<1..1464)
                     /locus_tag="VLL06_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HSS29675.1"
                     /translation="MASQRQTASASQPPILAEQQRLADDAQRQRHWKRWGPYLSERAW
                     GTVREDYSPYGTAWESFPHDQARSRAYRWNEDGLGGICDRHQLICFAVALWNGRDPIL
                     KERLFGLTGNEGNHGEDVKECYFYLDSTPTHSYMKYLYKYPQAAFPYTQLVEENRHRG
                     RQNPEFELIDTGVFDGNRYFDVFVEYAKATPEDLCIRIQVVNRGPEQAELTLLPTLWY
                     RNTWSWGSDIRRPRFKQGESKEQMSVIETQHEYYGLRRLLCKGEPTLLFTENETNTRR
                     LYGDQDGARYVKDSFHDYVVQGEKGAVNPGHLGTKAAAQYVLTLAPGATKTICLRFTN
                     DVRTPAFTKEAFDTVFTERLQEANEFYDRLAPGTLSEDARRVQRQAFAGLLWSKQFYH
                     YEINRWLKGDPIMPDPPRERLEGRNADWTHLYNADVISMPDKWEYPWYAAWDLAFHVL
                     ALSQVDADFAKHQLDLMLREDYMNPNGQIPAYEWNFGD"
     gene            complement(2247..2549)
                     /locus_tag="VLL06_01525"
     CDS             complement(2247..2549)
                     /locus_tag="VLL06_01525"
                     /inference="COORDINATES: protein motif:HMM:NF042904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6799 domain-containing protein"
                     /protein_id="HSS29676.1"
                     /translation="MVNYRNVTIAVVGLFLSVSFLASTVVAAEKDGIMMKNGRIQKLQ
                     EGKDIGPMDRMTTMANGTKVMMNGKIMTKDGQQSQLEEGQVMMLDGKLIDSKGSSK"
     gene            3692..3874
                     /locus_tag="VLL06_01530"
     CDS             3692..3874
                     /locus_tag="VLL06_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29677.1"
                     /translation="MSIQHLLAKTDMLKFWEYMFNACGEDFNCVLGWLLAVLLGFLAL
                     VMGFGYLISRRNRREQ"
BASE COUNT         1045 a         1267 c         1229 g         1039 t
ORIGIN      
        1 gtcgccgaaa ttccattcgt acgccgggat ctggccgttc gggttcatat agtcttcgcg
       61 cagcatcaag tcgagttgat gcttggcgaa atccgcatcg acctgcgaca gcgccaacac
      121 gtggaaggcg agatcccacg ccgcgtacca ggggtactcc cacttgtccg gcatggaaat
      181 cacatcggcg ttgtagagat gcgtccagtc agcattgcgg ccttcgaggc gttcgcgcgg
      241 aggatccggc ataatcgggt cgcccttgag ccagcggttg atctcgtaat gatagaattg
      301 cttactccag agcagaccgg caaacgcctg gcgttggacg cggcgcgcgt cttccgaaag
      361 agtacccggc gcgagtctgt cgtagaactc attcgcctcc tggaggcgct cggtaaagac
      421 tgtatcgaag gcctccttgg tgaacgccgg agtccgcacg tcattcgtaa agcgcaagca
      481 gatcgtcttg gtcgccccag gcgcgagcgt gagcacatat tgggccgcgg cttttgtgcc
      541 aaggtgaccc ggattgaccg ctcccttttc gccctgcacg acatagtcgt gaaagctatc
      601 cttcacatac cgggcaccgt cttgatcgcc atagagacgg cgggtgttgg tttcgttttc
      661 tgtgaagagc agggtgggtt cgcccttgca gagtaaccgg cgcaggccgt agtactcgtg
      721 ttgcgtttca atgacgctca tctgttcttt cgattcccct tgcttgaaac gggggcgtcg
      781 gatgtccgat ccccaggacc aggtgttgcg ataccacaga gttggaagca gcgtgagctc
      841 cgcctgctcg gggccacgat tgacgacctg gatacggatg cagaggtctt ctggtgttgc
      901 cttggcatac tccacgaaca catcaaaata tcggttccca tcgaagacgc cggtgtcgat
      961 caattcaaac tccggatttt gtcgccctcg atgccgattc tcctcgacca gctgggtata
     1021 ggggaaggcc gcctgtggat acttgtagag atacttcatg taggaatgtg tcggcgtcga
     1081 atcgagatag aagtagcatt ccttcacgtc ctcgccgtga ttgccttcgt tacctgtgag
     1141 accgaacaga cgttccttta aaatgggatc acgtccattc cataaggcca cggcaaagca
     1201 gatcagttga tggcgatcgc agatgccgcc gaggccatct tcattccagc gataggcacg
     1261 ggatcgtgcc tggtcgtgcg ggaacgattc ccaggctgta ccgtatggac tatagtcctc
     1321 gcgcacggtt ccccaggccc gttcgcttaa atagggcccc cagcgtttcc aatggcgctg
     1381 ccgctgcgcg tcatctgcaa gacgctgctg ctctgccagg attggtggtt gagaagcgga
     1441 cgcggtctga cgctgagagg ccatacaact ccttcggtag aaagggagca aatcaagagt
     1501 cggccgacgg cgaaacgttt tacagcaggc tagtaccggg ctggcgtgta gtcaagcgaa
     1561 tgcgatagaa cgatggccac ttcgtgatca gcggagtctg gtcatcctct ccttacatga
     1621 ccttcgaggt gaggtcccca gtttcgatcg ccgctgcgag cgtgccctct ggcgtcatga
     1681 tactcacttc gacaggtcct gcgccaaaaa catagccctg gcagtcaacc gtgaatccat
     1741 ctggctcgaa aaacggatct ctcctcccgc gcgtcgcgta ggagtagaca cggtcgttcg
     1801 tgacaggggt cgcgtacctc gaccacgtct taccgtggga aagcacagca cgtgaacgga
     1861 gcgcgaaagc ggcaggagca atgtcttgca atactcttct aattcgcttg aggagagggg
     1921 aaacgggaga gggtcctggg actggtagcc ggcgtggact cgagccttcg tctcctcgtc
     1981 atctggtgat cgctggctta ccgaagcggg caatatgggt aattcgcgac ggcattagaa
     2041 acccgccggc aggcagagtg ctctggataa acggttgaag caattccggt gagcggttgg
     2101 atggatgatt tcgcaatcat ccatccactc gctcggggat tcagggaaaa accaatgacg
     2161 cctggcaaac tgactgagca gaaccacgcc ctgctctgct cgttacgcgg acgtccggag
     2221 tcgtcggatc cgacgatgct gagatgctac ttgctcgagc ccttgctatc gatcagcttc
     2281 ccgtccagca tcatcacttg accttcttcc agctgggatt gctgcccgtc cttcgtcatg
     2341 atcttgccgt tcatcatgac cttggtgccg ttcgccatgg tcgtcatgcg atccatcggg
     2401 ccaatatcct tcccctcctg caacttctgg atcctgccat tcttcatcat gatgccatcc
     2461 ttctctgcgg ccactacggt ggacgcaagg aaagacacgg acaggaagag cccaacaact
     2521 gcgattgtaa cgtttctgta gttcaccatg gcttaactaa tcctccatta ataattagtg
     2581 agtggtgtga atgaactgcc ctgactacgt tgaacctgga acgggaaggc gggcctcggc
     2641 ccgcttgagc tgatgtgagg ccgacatcgt taccccgtcg ccgacgttcg atcgaatcga
     2701 cgtcccgcct ttttcgtcgt ggttgcacag gtacgggatt tcgtggttgg tcccgggatg
     2761 tgtgcaccaa caaagcagac agaggcagca actgcagcag tggacgcaag ctcaccggca
     2821 caactcgacg cacacgactg cgcattgcag atctctccat cagcttgtcg aatggctcgt
     2881 cgaatttctc gatgcggtca tcaggcgatc gacgaccttt ctacactgtg aatgcccact
     2941 gttccgcaac aaccatccgc cttatcgctc aaggcacatg ctcgttgcgc gatgctcgtg
     3001 gatgctccag cgcgcatctt caaccgtttc gctcgcccca caacaggcag acaacggtct
     3061 ggatgattgt ttcgacgcga actttcacac gcgccggtca tcagccgcca cgccgcaaca
     3121 cacgtcgtgc tcggtgacga cgctgaccag catgaggggg taagggcata tattgttcaa
     3181 tagtgtctta gggcttgtca agtgccccat tattattctc cgtggcagtc tgatattgac
     3241 agaaatattg ctggcatatc acttcgccgg agccagccat gtcgacacag tagccacaat
     3301 ccaatactac tatgtagacg aatatgtaga cgaagctgcg gtcggattta acagactcaa
     3361 ccacaggctc ttgtatacaa atcgcgggaa tagacgatca caaacccgct aaaaaagtgt
     3421 taggatgaca tcgaacgggg gcctgacccg tcatgaggcc ggacaagatc aggagcaagg
     3481 acgacaccgc caagagaatg tctatgtccg ccgaacgccc ccattacacc aagaacccac
     3541 aacgcagagc cagtccgtac cgaaatcacc ggaaactttt cggacttgtc tgcagaaatc
     3601 atgaactctg ccagctccct tccctctgcc tgtcaagccc agtacggaga tgctatacta
     3661 gcgcccaaga aaagggccaa ggagcagcac gatgtctata caacacctac tagccaagac
     3721 cgatatgctc aagttttggg agtacatgtt taatgcctgc ggagaggatt ttaactgtgt
     3781 gctcggatgg ttactggcag tactcttggg gttcttggca ctcgtaatgg gttttggcta
     3841 tttgatctcg cggcgtaata gaagagaaca gtaggccacg cgcgcgcggt accacgggta
     3901 tcgcgtggaa gccagacatt cggcacaacg gaattctccc acccgcttca accagcacac
     3961 atacatagag cgatagtgac cgcgcaaaga gatgcccaga atgaccactc cacctacaca
     4021 aacggctacg gaaccttgcc gcaaatgcag caaacctctc gaatgtgcac agtacttttc
     4081 cagtgacagg cgttcatcta gtaccatgtt cgaaggaaaa gagggacttc gatctaccac
     4141 aagtgttagt catcgcccct gtccacattg tcgggatccc aagcctcttt ttgcactaga
     4201 actcaaaacc tataagagtg cgatcctgcg gttactgatt gtggctgcgc tatgcgggct
     4261 ctactttgtt gcggatccac aaatctttga tggggagaat aaaaatctca attcttttct
     4321 tacgtggacg agtatataca caggaggtct attcgtcttc tttgccattc ttaatggagt
     4381 gtcgtatttc atgaaaaaga aggagggata atacagcaag gcgttgccag cctgatcagg
     4441 catgtcatct caattctgcc gcacagacag ggaaatcttg ataggtcgta tcgataccat
     4501 aaaaaggcgg cagatcgtaa atgcgcagtg ttgcgtgttg cccatcgttt aaatatggac
     4561 ataatccctt tgccccccgt
//
LOCUS       DATIPF010000057         3111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_185663, whole genome shotgun sequence.
ACCESSION   DATIPF010000057 DATIPF010000000
VERSION     DATIPF010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185663"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1241)
                     /locus_tag="VLL06_01535"
     CDS             complement(114..1241)
                     /locus_tag="VLL06_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSS29678.1"
                     /translation="MTTSRKTKAPLTHIRYAVVGLGHIAQVAVLPALAHASSNSQLTA
                     LVSGDPKKLKDLSRRYHVPYTYSYEQYDKCLREGHIDAVYIALPNNMHCDYATRAAKA
                     GIHVLCEKPMAVNEQECRQMIRAAEQAGTRLMIAYRLHLEETNVNAVEIARSGTLGKL
                     RIFTSLFTLQVRDGDIRIRRKMGGGSLYDIGVYCINAARCLFQDNPIEVSAFSVKGTD
                     RRFNEVDEMSGALLRFPGERLASFICSFGAADRSSYELIGSKGRLQVDPAFDYVGPHT
                     YHLTLNGKTRTRRFSPGDQFAAELIYFSDCILHKKNPEPSGLEGLIDVQVVRALYRSA
                     ALRRPVKLAPEAKVRWPTKEQVIRRPPVRKPMLVHTQSSSL"
     gene            complement(1277..1582)
                     /locus_tag="VLL06_01540"
     CDS             complement(1277..1582)
                     /locus_tag="VLL06_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29679.1"
                     /translation="MAVEVQSAPTLPEMAEAKDQHEMDSLKEFGKCAVGLRNVLYQDR
                     ALSEGEFHFMDNHFQALEMAYLRWKRKHGTAQGIRSDAVLPMTPMPLSGQTSRTNPS"
     gene            1850..>3111
                     /locus_tag="VLL06_01545"
     CDS             1850..>3111
                     /locus_tag="VLL06_01545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSS29680.1"
                     /translation="MKDHAGRSSSVWMTTAMLPRFAKLGENEKAEVMIVGAGIAGLTT
                     AYLLGQAGKSVIVLDDGPIVSGETERTTAHLVTALDDRYFDLERLHGEKGAQLAAESH
                     SAAIDHIERIVQHEHINCDFERLNGYLFVPPGEPTDILDRELAAAQRAGLRELHMVGR
                     APLTSFDTGRALCFPRQAQFHPLKYLAALSQAIIRDGGRIYTETHVAKMEGGKSARIE
                     TEDGRTVTAGAVVVATNTPVNDVLAVHTKQAAYRSFVIGAAVPVGSITKGLYWDTSVP
                     YHYVRLSHSFSTGRDILIVGGEDHKTGQADDAEFRYQRLEKWARARFPMMTAIEFRWS
                     GQVIETVDGLAFIGRNPLDEPNVYIATGDSGNGMTHGTIAGILLTDMIQGRDNAWSTL
                     YEPARKTLGALKTFAKESLNMAAQYADWV"
BASE COUNT          696 a          828 c          866 g          721 t
ORIGIN      
        1 gaccctccga gttgagggtg agtgtttagc catcactgta cggcccgcga gtaatcgacg
       61 gacctaggaa ggcgcctagc ttttggcgag cccgaccagc caatggtctg gagttataag
      121 gacgatgact gcgtatgtac gagcataggc ttacgaacag gaggacgccg gatcacctgc
      181 tctttcgtcg gccaccgaac tttggcttca ggcgccagct tgaccggcct tcggagcgcc
      241 gctgagcgat ataatgcccg aacgacctgg acgtcgatca aaccttccag cccagagggt
      301 tcaggatttt tcttgtgaag gatacaatcg gaaaaataga tgagttctgc cgcaaactga
      361 tcgcctggtg aaaaccgcct agtccgggtt ttaccattga gcgtcaggtg atatgtgtga
      421 gggccgacat agtcgaacgc gggatcgact tgcaggcggc ccttgctccc aatgagttca
      481 taggaggagc ggtctgccgc gccgaaactg cagataaaac tggcaagtct ctcaccggga
      541 aaacgaagga gcgcaccaga catttcatcg acttcgttga acctccggtc cgtgcctttc
      601 actgagaaag cggatacttc tatcggatta tcctgaaaca gacagcgggc cgcattaata
      661 caatagacgc cgatgtcata gagcgaaccg cctcccatct tccgtcgaat cctgatgtcc
      721 ccgtcgcgca cctgcaacgt aaagagagag gtaaagatgc gcagcttgcc aagcgtgccg
      781 gagcgggcga tctccacagc gttcacattg gtttcttcca aatggagccg ataggcaatc
      841 atcaatcttg ttccggcctg ctcggcagcg cgaatcatct gtcgacactc ttgctcgttc
      901 acggccatag gtttctcgca gagcacgtgg atcccggctt tggcggcgcg cgttgcatag
      961 tcgcagtgca tattatttgg cagggcgatg tagaccgcat cgatatgccc ttcacgcaaa
     1021 catttgtcat attgctcata ggagtacgtg tagggtacgt gatagcgcct gctgaggtcc
     1081 ttcaactttt ttggatcacc ggaaaccagg gctgtaagct gggagtttga ggaggcatgg
     1141 gcaagtgccg gcagcaccgc cacctgggcg atgtgcccca acccgaccac ggcgtatcgg
     1201 atatgcgtca ggggtgcctt ggttttcctg ctcgtggtca tctcggtctc tattttgtta
     1261 agcgatagct gactacttat gaaggattcg ttctgctggt ttggccggaa agcggcatcg
     1321 gggtcattgg gaggactgca tccgatctaa tgccttgcgc cgttccgtgt ttccgtttcc
     1381 atcggaggta ggccatctct aaggcttgga aatgattgtc catgaaatgg aattctccct
     1441 cgctgagtgc tctgtcctga tacaggacat tgcgcaaccc caccgcgcat ttcccaaatt
     1501 ccttgagact gtccatttcg tgttggtcct tcgcttccgc catctcagga agtgtaggag
     1561 ccgattgaac ttcaacagcc atgagtcacc tccttcatca ctcacactca gcgactctcg
     1621 caatatttgt ccccttgtga tgagtcctac catagtcccg cgcggtcgcc cctgccacta
     1681 ggcgatcccc cagtgctact cgttccatgg gcctacgcag tgagactcgg cctattccta
     1741 gtcctcagta ttccgggagc tcagtacagt ggtattgatt cggacatacg cgatgcaaca
     1801 gggtcgggcc tctgattccc cagatggtgc actgattcgg agaccgtaca tgaaagacca
     1861 tgcgggacgg agcagttcgg tctggatgac cacggccatg ttgccgaggt ttgccaagtt
     1921 gggtgagaat gaaaaagctg aggtcatgat tgtaggcgcc gggattgccg gcttaaccac
     1981 cgcatatttg ttgggacagg ctggaaaatc cgtcattgtg ttggacgatg gcccgattgt
     2041 gtcaggtgag accgaacgga caaccgccca tctcgtgacg gcattggacg atcgttattt
     2101 cgatctggag cggttacacg gagaaaaagg ggcgcagctc gcagccgaaa gtcattctgc
     2161 agccatagac cacattgaac gcatcgttca acacgaacac attaattgtg actttgagcg
     2221 tctgaacgga tatctctttg tgccaccggg agaacccacc gacatcctcg atagagaatt
     2281 agccgcagca cagcgtgccg gcttgcgtga actccatatg gttgggcgtg cgcccctgac
     2341 ttcattcgac accggccggg cactgtgctt tccacgccaa gcacaatttc accccctcaa
     2401 atatttggcg gcactgtccc aggcgatcat cagggatgga ggacgcatct atacagaaac
     2461 gcacgtcgca aaaatggaag gcggaaaatc agcccggatc gaaacggagg atggtcggac
     2521 tgtgactgca ggggcggtgg tggtggcgac caacacccct gtcaacgatg tcttagccgt
     2581 tcacaccaaa caggctgcgt atcgctcatt cgtgattgga gcggctgttc cggtgggctc
     2641 catcacgaaa ggattatatt gggacacctc agtcccatac cactatgtac ggcttagtca
     2701 ttcgttctca actgggagag atatcttgat cgtcggtgga gaggatcata agacggggca
     2761 ggctgatgat gccgagttcc gttatcagag gttggagaaa tgggctcgcg cccgttttcc
     2821 catgatgacg gcgattgaat tccggtggtc gggtcaagtc attgaaactg tcgatgggct
     2881 ggcatttatc ggacggaatc ctctggacga accgaacgta tatattgcga ctggtgattc
     2941 tggcaacggt atgacgcacg ggaccattgc cggcatacta ctcaccgaca tgattcaagg
     3001 tcgcgacaat gcctggtcga ccctgtatga gcctgctcgc aagacgctcg gtgcattgaa
     3061 gacatttgcc aaagaatctc tcaacatggc cgcccagtat gctgattggg t
//
LOCUS       DATIPF010000058         2926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_981230, whole genome shotgun sequence.
ACCESSION   DATIPF010000058 DATIPF010000000
VERSION     DATIPF010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981230"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VLL06_01550"
     CDS             <1..322
                     /locus_tag="VLL06_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29681.1"
                     /translation="RNGMNTFTRILLTIAALGFGSGNVGAADPMADQEARPTFAERLT
                     KDTVKGTLMRRDGEFYWIKDHDNKEIKLHVDTSTKIDKLLAGDQVKAYVTESGHTTTL
                     QRDE"
     gene            709..2841
                     /locus_tag="VLL06_01555"
     CDS             709..2841
                     /locus_tag="VLL06_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HSS29682.1"
                     /translation="MVIQDQVPTHHFQTGRNCWRIEESHRVSFLIDGEAYFRAFRAAA
                     TRARRSIMILGWDFDSRTRIVIDQDPDGLPDHIGKFCQELLRRQHQLQIYVLTWNHHL
                     IYSIEREWTSYASWLIPRRLHTMKDGAHPVGASHHQKVVVIDDALAFVGGLDFAPCRW
                     DTPAHQINHPQRRLLRGKPCRPFHDVQIMVDGEAARALGELARARWEGATGEQLFPSV
                     LESVGNHWPRSVTPDLHHVPVAIARTQPNYENQKEIREVEALFTESIKLAHRFIYIET
                     QYLTSHVIASALADRLRDPAGPEIVILLHPNCDGWLEHHTMDVLRGRVLKTLRADDRF
                     HRLALYFPHLPGLDNECMTLHAKVCVIDDDFVRVGSANCSNRSMGFDTECDLAIESSG
                     NAEIKRSIAGFRNRLLGEHLDVTSEQVADAFAREGSLIASIETLRGKPRTLRVFDEQI
                     PADVDDWIPAAEYIDPIRPYESALVLHASRRSVRRQLIIGASVLAGLLAVAAGWHWTP
                     LAQLLNIPMLVGYAEQFKQSAVAPFLALAAFVLGALMVVPVTAMIAVTVLAFGPVLGF
                     SYALIGMTVSALLTFWIGKLLGRHSIQRLAGSRLHQLSRRLAQKGVVAVIMIRLVPVA
                     PFTIVNLIAGASHIRFRDFALGTVLGELPGLLAISIFVNQISDTIRYPTVTSISMLTV
                     IALLLFLGSIGLWRWLNKRTPSTHPLTR"
BASE COUNT          662 a          821 c          789 g          654 t
ORIGIN      
        1 gaggaatgga atgaacacct ttacacggat actattgacg atagcagcgc tgggattcgg
       61 atccgggaat gttggagccg ccgatcctat ggcggatcaa gaagcgagac cgactttcgc
      121 ggaacgcctg accaaggaca cggtcaaagg cacgctcatg agaagagatg gggagtttta
      181 ctggatcaag gatcatgaca ataaagaaat caagcttcat gtcgatacga gtaccaagat
      241 cgataaactt ctggcgggcg atcaggtcaa agcctatgtc actgaatctg gacataccac
      301 gacgcttcag cgcgatgagt gatcgatcac gcaatcggga aatgctgaaa cacttaatgt
      361 gggcattatc ccgacaggct ctcaattcgg tacgagccct accatccact tgttaatcgc
      421 tgccccgtca ctcattacac ttcggcggcc agggctgaga gccaagaagc acctgcttca
      481 gactgggcat tttcctagta cgcaccggcg catggataca atatagtaaa aatgcttcgg
      541 gaacacgcaa tgcacacact gtgaatgcac gacggccacg tcgtgaacgc acagaatacg
      601 cagcgcggga cccgacgcaa aaggctcggt tccgaaaggg gtcgggcctt tgtattgtcc
      661 agggtatgcg ttgaaggctt gtgaattatc tcaacctcga tatccatcat ggtcattcag
      721 gatcaggtac ccacacatca tttccagact ggccgaaact gttggcgcat cgaagagtct
      781 catcgtgtct ccttcctcat cgacggtgag gcatacttcc gtgccttccg tgcagcagcg
      841 acccgcgctc ggcggtcgat catgattcta ggatgggatt tcgacagccg gacccgaata
      901 gtcatcgatc aagacccgga tggactcccc gatcatatcg gaaagttttg ccaagaactt
      961 ttgcgccggc agcaccaact gcagatctac gtgctcacat ggaatcatca tctgatttat
     1021 agcattgaac gtgaatggac ctcctatgca agctggctca tccctcggcg gttgcatacc
     1081 atgaaggacg gcgcgcatcc cgtcggcgcg tcccaccatc agaaggtcgt cgtcatcgat
     1141 gatgcgctgg cattcgttgg ggggttggat tttgcgccct gccggtggga tacccccgca
     1201 catcagatca atcacccaca gcgtcggctc ctgcgtggga aaccctgccg accatttcat
     1261 gatgtccaaa taatggtgga cggcgaggcc gctcgcgccc tcggagaatt ggcgcgggcg
     1321 cgatgggaag gcgcgaccgg tgaacagttg tttccgtccg ttctcgaatc ggtcggaaac
     1381 cattggccgc gttcagttac acctgatttg caccatgtcc ctgtcgccat cgcccgtaca
     1441 caaccgaact atgagaatca aaaggagatt cgcgaagtcg aagcgctgtt tacagagtcg
     1501 atcaaactgg cgcatcgatt catctatatc gagactcaat acctcacctc gcatgtcatc
     1561 gcgtcggcgc ttgcggatcg cttgcgagat cccgcgggac cggaaatcgt catcctgctg
     1621 cacccaaact gcgacggttg gctggagcac catacgatgg atgtattgcg cggcagggtg
     1681 ctgaaaacac tgagagcgga tgaccgcttc caccggttgg cgctctactt tccacacctt
     1741 cccggattgg acaatgaatg tatgaccttg catgcgaagg tctgtgtgat cgatgacgat
     1801 tttgttcggg tcggatccgc caattgcagc aaccgctcga tgggattcga taccgaatgt
     1861 gatctagcga ttgaatcttc cgggaatgcg gagatcaagc gaagcatcgc cgggtttcgg
     1921 aatcggctcc tcggtgaaca cctcgatgtg acatccgagc aggttgccga cgccttcgcc
     1981 cgcgaaggat cgttgatcgc atcaatcgag acattacggg gaaagcctcg cacgttgcga
     2041 gtttttgacg aacagattcc tgccgacgta gacgattgga ttccggctgc cgagtacata
     2101 gaccccatca ggccctacga gtcggcactt gtgctgcatg cctcccggcg gtcggtgcgc
     2161 cggcaattga tcataggtgc ctccgtgctg gcgggattgc tggccgtggc cgctgggtgg
     2221 cattggacac ccctcgcaca gttgctgaac attccaatgc tagtgggcta tgccgaacaa
     2281 ttcaaacaga gtgccgtcgc tccgtttctg gcattggccg catttgtcct cggagcattg
     2341 atggtcgttc cggtcacggc gatgatcgcc gtgaccgtcc tcgcattcgg gccggttcta
     2401 ggattttcct acgcactcat aggcatgacg gtaagcgccc tcctcacctt ttggatcggc
     2461 aagctcctcg gaagacactc gatccaacgg cttgccggat cccgactaca tcagctcagc
     2521 cgccggctgg cacaaaaagg agtggtggcg gtgatcatga tccgcctcgt gccggtcgcg
     2581 ccttttacga tcgtcaacct catcgcggga gcctcccata ttcggttccg tgattttgcc
     2641 cttggcactg tactgggaga attgccaggg ctattagcca tttcgatctt cgtcaatcaa
     2701 atcagcgata ccatcagata tccaactgtc acgagcatca gtatgctcac cgtgattgcc
     2761 ttactcctct ttcttggaag catcggccta tggaggtggt tgaataaacg taccccttcg
     2821 acacatcccc tcacgaggtg agcaagcgag ttatgcgcct ggatctctcc acatacaaca
     2881 tccactcctg tatcggcgga gacaggcgct atgacccgga aagaat
//
LOCUS       DATIPF010000059         4086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_362614, whole genome shotgun sequence.
ACCESSION   DATIPF010000059 DATIPF010000000
VERSION     DATIPF010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362614"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VLL06_01560"
     CDS             complement(<1..295)
                     /locus_tag="VLL06_01560"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HSS29683.1"
                     /translation="MFTRFEEFAATQMLGQPDSPPRSEGKLFFSEDWQRTVFGMALAL
                     SKEGHFEWEDFRQHLIQKIAAWEQDACNGEARWDYYEHYTLALIEVLEQYGVLK"
     gene            complement(304..927)
                     /gene="nthA"
                     /locus_tag="VLL06_01565"
     CDS             complement(304..927)
                     /gene="nthA"
                     /locus_tag="VLL06_01565"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:TIGR01323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HSS29684.1"
                     /translation="MSGLFEYDKDREAASAAKVRALEALLIQKGVLGSDSVDTVLRHF
                     ETVAGPFNGAHIVARAWLDPAYKRRLLEDTPKAIAELNFPGMTGAEGEHMKAVANSES
                     VHNLIICTLCSCYPWPVLGLPPYWYKDPVFRSRGVREPRAVLREFGVPIPPSKEVKVW
                     DSSAQIRWFVIPERPAHSEELNEEQLAALVTPEAMMGVALVPPPQAA"
     gene            complement(924..1694)
                     /gene="nthB"
                     /locus_tag="VLL06_01570"
     CDS             complement(924..1694)
                     /gene="nthB"
                     /locus_tag="VLL06_01570"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:TIGR03888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HSS29685.1"
                     /translation="MRLQHYLGGLENLGPVNFFDKKVFMMEWEKRIFGIHTVMMAESG
                     HLEHALQKYPIKDLPTTFKDTWTWASLRTGAENMQPFEYFKYRYYEKWLMGISQFFVD
                     RGYIAADELAALTEHYHMNPDAPLPARPNPALKEQVVKYLHHGDSGLRMPIAPARFSV
                     GDIVYVADPKAADHTRLPGFLRNKHGTVSEVYPGAFEYFVSTGPDGLEGPQHVYCVSF
                     DATHIWGTDKSEPHTVIYADLFDAYLLSAHEFHSKDPS"
     gene            complement(1697..2707)
                     /locus_tag="VLL06_01575"
     CDS             complement(1697..2707)
                     /locus_tag="VLL06_01575"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSS29686.1"
                     /translation="MLSKKIPVTVITGFLGAGKTTLVNHLLTQHHGANIGVIVNEFGE
                     VGVDGELIVADEEALIEIRNGCICCTVRTDLVAGVKALLERNDITLDRLIIETSGLAD
                     PAPVLQTFLADTDLLTRVELESVVALVDAAHVQRQIGNDIAREQIAFADLIMLNKVDL
                     LEAEGVAPLERHLRAINPTAAIIRTTNAEVAAGTLLGVSRFSLPNLLAIEPGILRDEH
                     DHQHDNSITSCAVETPDSLDPDRFHRWVNRLVQQQGQHIMRMKGVLNFHDEARQFYFH
                     SVHMLLEAKPGKRWQSAEARQSRFVFIGRHLDSTGLPRGFLDCVHDSARNPSPSLSMN
                     RI"
     gene            3230..>4086
                     /locus_tag="VLL06_01580"
     CDS             3230..>4086
                     /locus_tag="VLL06_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HSS29687.1"
                     /translation="MLTTLRAVLDNAEHRRVAVGHFNISDLVAMKAIGETARELHLPV
                     LVGLSEGERDFMGVRQAVALVHSLRDHDNFPMFLNADHTHSLAKAIDAAKAGFDAISF
                     DGSQLPFDENVTQTKQAVEAIKTINPSIIVEGEIGDIGSGSEIHETIPEISKILTTPE
                     QAKQFVDATGIDVLAPAVGNMHGLLPGMIRGQMKKRLHVQRVRDIKEATGIFMTLHGG
                     SGTDDEDFKSAVKAGITIVHVSTEMRLAWRQGLEAALAKAPDELVPYKILREALGAVK
                     KVVHARLLL"
BASE COUNT          900 a         1057 c         1190 g          939 t
ORIGIN      
        1 gcttgagtac tccgtattgt tcgagcactt caatcagtgc cagggtatag tgttcataat
       61 agtcccacct ggcctcgcca ttgcaggcat cttgttccca cgcagcaatc ttctgaatga
      121 gatgttgcct gaaatcttcc cactcgaaat ggccttcttt ggacagggcc aacgccatac
      181 cgaacaccgt gcgctgccag tcttcgctaa aaaacagctt tccctcactg cgtggtggcg
      241 agtccggttg cccaagcatc tgcgtcgcgg cgaattcttc gaatcgagta aacatgtggt
      301 gtgttatgcg gcttgcggtg gaggaaccag agcgaccccc atcatggctt ccggtgtgac
      361 cagggccgcg agttgttctt cgttcagttc ttcggagtga gccggtcgtt ccggaatgac
      421 gaaccagcgg atctgcgcgc tgctatccca gactttgact tccttgcttg gcgggattgg
      481 tacaccaaac tcgcgcaaca cggcgcgagg ttcgcgcacg cctcgcgagc ggaataccgg
      541 atctttatac cagtagggcg gcaggcctag caccggccat ggatagcagg agcagagggt
      601 acagatgatc agattgtgca cggactcgct gttggccacg gctttcatat gctcgccttc
      661 cgcgccggtc ataccaggga agttgagttc ggcgatcgcc ttgggggtat cttccagcaa
      721 acgacgtttg taggctgggt cgagccacgc ccgggccacg atatgagcgc cgttaaacgg
      781 gccagccacg gtttcgaaat gacggagtac agtatcgacg gaatcgcttc ccagtacccc
      841 cttttgtatg agcagcgctt ccagcgcgcg gactttcgcc gcactggcgg cttcgcggtc
      901 cttgtcgtac tcaaagagtc cactcatgac ggatccttcg agtgaaattc gtgagcagac
      961 agcaggtagg catcaaacag gtcggcataa atgacggtgt ggggttcgct tttgtcggtg
     1021 ccccagatat gcgttgcgtc aaacgacacg caatagacat gctgcggccc ttccaggccg
     1081 tccggcccgg ttgagacaaa atattcgaac gcgccaggat agacttcgct gaccgtgccg
     1141 tgcttgttgc gcaagaatcc aggcagtctg gtgtgatcgg cggctttggg gtcagcgaca
     1201 tacacgatgt ctccgacgga aaagcgggcc ggtgcaatag gcatacgcaa gccggagtcg
     1261 ccgtgatgga gatacttgac cacctgttcc ttgagtgccg ggttgggccg tgctggaagc
     1321 ggtgcatcgg gattcatatg gtagtgctcg gtcaatgcgg ccaattcatc ggcagcgatg
     1381 tagccgcgat cgacgaagaa ttggctgata cccatcagcc atttttcata ataccgatat
     1441 ttgaagtatt cgaacggttg catgttctcg gcaccggtac gcaatgaggc ccaggtccag
     1501 gtgtccttga acgtggtggg tagatccttg atcggatatt tctgcagcgc atgctccaaa
     1561 tggccgcttt cggccatcat cacggtgtga atgccaaaga tgcgcttctc ccactccatc
     1621 atgaatactt ttttgtcgaa aaaattgacc ggtccgagat tttccaggcc gccgagatag
     1681 tgttggagtc tcatgatcaa atccgattca tggacaggct aggcgaaggg ttacgtgcgc
     1741 tgtcgtgtac gcaatcgagg aacccgcgcg gcagccccgt gctgtcgaga tgacggccga
     1801 taaacacgaa tcggctctgg cgcgcctcag cagactgcca gcgctttccg ggcttggctt
     1861 ccagtagcat gtgcacgctg tggaaataga actgccgcgc ctcgtcgtgg aaattcagca
     1921 cgcctttcat gcgcatgatg tgttggccct gttgctgaac caatcgattg acccagcgat
     1981 gaaagcggtc cgggtcaagc gagtcggggg tttcaactgc acaggacgtg attgagttat
     2041 cgtgttgatg gtcgtgttcg tctcgcagga tgccgggctc tatcgccagc aagttgggta
     2101 gtgaaaatcg ggacacgccg agcaatgtac cggctgccac ttcggcgttc gtggttcgaa
     2161 tgatggcagc ggttggattg atcgcgcgca gatggcgttc cagcggcgcg acgccttcag
     2221 cctccagcaa atcgacttta ttcagcatga tcagatcggc gaaggcaatc tgttcgcgcg
     2281 cgatgtcgtt cccgatctgg cgttggacat gggccgcgtc taccagggcc acaacggatt
     2341 ccaactctac gcgagtcaag agatctgtat cggccagaaa cgtctgcaac accggcgccg
     2401 ggtcggccag ccctgaggtt tctatgatca ggcgatccag tgtgatgtca ttgcgctcca
     2461 gcagggcttt gacgccggcc accaggtcgg tgcgcacagt acagcagatg cacccgttgc
     2521 ggatctctat tagggcttct tcatcggcga caatgagttc gccgtcgact ccaacctctc
     2581 caaactcatt gacgatcacc ccgatgttgg cgccgtggtg ctgtgtgagc agatgattga
     2641 caagagtcgt cttgccggca ccgagaaatc cggtgatgac cgtgacaggt atttttttcg
     2701 acagcatagt atctgaacca aggatcacac ggttagtttg acaggcctcg ggggacacac
     2761 atcgattcgt taaaataatt ggggcgtgag aactcggcgt ctacatgtgt gtttgtgaca
     2821 ttgacttggc gtaagtggag tgccatcacc atacacccaa cctttgatta gccagacgca
     2881 cgtagacggt aagaacggca tacggaacgc gacaagatta tcttgtgcca ggctcaccgc
     2941 tattgtcaac gggttgtagc ggtaccaggg caattgggtc tcttgagtgg gctcagtcgc
     3001 gtggggggtc gaatgattgt catatcgcga atgccctgtc cattttcatc gttggccgct
     3061 gtcatacgtg gaagacttac gatgaactgc ccattcaccc catagaacaa gagtcgatcg
     3121 tatggcacac tatgcagcac cgaaaaagtg ttctcaaggt aggcaggcgt ctcaactacg
     3181 agccagttga ccaacataag cgggatggga tctgagggag ggagaagaca tgctaactac
     3241 gttgcgcgcg gtgcttgata atgcggagca tcgacgggtg gccgtgggtc attttaacat
     3301 ctcggatctt gtcgcaatga aggccatagg cgaaaccgca cgggaactac atctgccagt
     3361 tttggttgga ctctctgaag gcgagcgtga ctttatgggt gtacgtcagg ccgttgcact
     3421 tgttcacagc cttcgtgatc atgataactt tccgatgttc ctgaatgccg atcatacaca
     3481 ctccttggcc aaggcgatcg atgccgcgaa agcgggattt gatgccatta gttttgacgg
     3541 gtcgcaactg ccctttgacg agaacgtcac gcaaacaaag caggctgtcg aagccatcaa
     3601 aacgatcaat ccgtccatta ttgtggaagg ggaaatcggg gacattggga gcggctccga
     3661 gattcacgag acgattcctg aaatctcaaa gatcctcaca actcccgagc aggcgaaaca
     3721 gtttgtcgat gcgacaggca tcgatgtctt agcacctgct gtagggaata tgcacggact
     3781 tcttcccggg atgattcgcg gacagatgaa gaaacgtctt catgtccaga gggttcgtga
     3841 catcaaagag gccacaggga tttttatgac cttgcatggg ggatcgggaa cggacgatga
     3901 agatttcaag agcgcggtca aggcaggcat caccattgtg cacgtcagta cagagatgcg
     3961 gctggcttgg cgtcaaggac ttgaggctgc gcttgccaaa gcgccggatg agcttgttcc
     4021 gtataaaatt ttgcgggaag cgctcggggc cgtgaagaaa gtggttcatg cgcgactgct
     4081 attgtt
//
LOCUS       DATIPF010000060         5401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_627845, whole genome shotgun sequence.
ACCESSION   DATIPF010000060 DATIPF010000000
VERSION     DATIPF010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627845"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VLL06_01585"
     CDS             complement(<1..414)
                     /locus_tag="VLL06_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSS29688.1"
                     /translation="MESIGEFFRQVRETKGLTIDEVASKTRIRTDFVKALEDGNFAKL
                     PDQVFARGFVRSYARSLGLDEDDAIHRFDQSAGAYYDKHVERERLRVRQAEEERKRKA
                     NRKAVAIAIGIAIITLIFLLSREQSSLLVRRSSSDV"
     gene            complement(425..1114)
                     /locus_tag="VLL06_01590"
     CDS             complement(425..1114)
                     /locus_tag="VLL06_01590"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS29689.1"
                     /translation="MTQIHCAGSWIRPFVGLVLVLALLIGWLPGCATSEGSAQKSKGY
                     YQEGVASLEQDQQKAFVAFQKAVTLDPDNKEARYGLGHILALQGKLPQAEQEFRAATA
                     LDDSYSEAYTYLGQVLEKQGRWKEAIAAFRRALTNPLYVTPDLARFHLGRVLAHENDY
                     QAAMEAFEDALVVNPPNVPPAQLHLELGRVYYKLGFERRSREELTKVTTLDKGGEYAA
                     AAKELLTRIKP"
     gene            complement(1111..2025)
                     /locus_tag="VLL06_01595"
     CDS             complement(1111..2025)
                     /locus_tag="VLL06_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSS29690.1"
                     /translation="MGKLLVVGSVALDTVKTPFGEVSEVLGGSATFFSTAASYFTSVD
                     LIAVVGEDFPPQHLAFLKSRGIDVTGLERRPGATFRWKGEYSHQLNEARTLDTKLNVF
                     ETFRPKIPEAYRSPDLLFLGNIDPELQLDVLQKLPRPPVVACDTMNFWINGKRDALWR
                     VLEQVDILIINDGEARALGEDTNLVKVAQKVLARGPKHLIIKRGEYGVLMFNEKQVFG
                     APAFPLEDVRDPTGAGDTFAGGFLGYLTATGNRSVEAFKQAIIFGSVMASFTVEAFSL
                     DRLRILDYKEVEERFRAFKQLTHFEDIS"
     gene            complement(2029..2928)
                     /gene="mtnP"
                     /locus_tag="VLL06_01600"
     CDS             complement(2029..2928)
                     /gene="mtnP"
                     /locus_tag="VLL06_01600"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HSS29691.1"
                     /translation="MTRQRSVSRADIGIIGGSGLYEIEGFRKVKELAVKTPFGPPSDK
                     VLVGELNGVRIAFLSRHGRGHWINPSEINYRANIYALKWLGVRQVISVSAVGSMKESI
                     QPGDVIVPDQFIDLTKRRVSTFFEGGIVAHVAFGDPVCASLGEVLLAAARTVGATVHQ
                     GGVYLCIEGPQFSTKGESRLYRQWGASVIGMTNLPEAKLAREAELCYSTVALATDYDC
                     WHETEEAVTVEAILTTLRQNVTLAKRLLRAALPGVADVKACSCQHALQHAIITAPDRM
                     PASLRRKLALLMDRVVPAQKGIH"
     gene            complement(2925..3926)
                     /gene="miaA"
                     /locus_tag="VLL06_01605"
     CDS             complement(2925..3926)
                     /gene="miaA"
                     /locus_tag="VLL06_01605"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HSS29692.1"
                     /translation="MAIPARAMCRLSETQVHCKPVIVLIGPTAVGKSRVAVEVAKAFE
                     TEVLTAYSRQVYRGMDVGTDKPTPEERQGVPHRLIDLVNPDESFNTGLYRRQALDDIE
                     RLYRGCRLPLVVGGTGLYVRTLLRGLCDAPPTDPIVRGALRQDVRDHGHDRLYARLVA
                     VDPVVAARLHPRDESKVIRALEVHQVSGRRMSEYQQEHGFAERPFSALIIGLNRDRDA
                     LYRRIEERIDWQLAHGLIEETKQLLARGYRRDSAAMKGLGYRQVAEHLAGEYDATEMV
                     RRFKRDTRHYSKRQMTWFRKEPGIQWLTIEESESVQHTAALVTAQVDRFLATLGDPG"
     gene            complement(3926..>5401)
                     /gene="mutL"
                     /locus_tag="VLL06_01610"
     CDS             complement(3926..>5401)
                     /gene="mutL"
                     /locus_tag="VLL06_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HSS29693.1"
                     /translation="ATRQDTVGSQLTLVAGAGRAIVDAPAVPGTRIDVSNLFFNQPAR
                     KKFLKSATTEFSHISHVVQLAGLAWPSVHFRLTHNGQEILNYPAVTADRDRILQVYRQ
                     AFLDRTIEVRGRIAGCSIRGVMVDPLHARASRTPQELFVNRRPIRNATVFHAVMDGYG
                     SFLPKGHHPTYVLFLDLEPDRLDVNVHPTKKEVRFAETEALHQLVRQSVRHALGGPER
                     KVVLGLTQAGLSQTIPEFAPTTGRQTSSSGYFHPAPLTATLRQDARNKVSDTVPLSGT
                     VEGNQLAFAHEATAAYQQAERPGIVPLGQILRTYLVAQVGDELQVVDQHTAHERVLFQ
                     RLWREWQSRDIPSQPLLIPEPIELSAAQNTLLLKRRDDLEKLGLLIEPFGTTAVAIRG
                     VPVGLGKVDAAVLVQDLLDDLIEWDTASSLELRVQPVLASLACHGAVRAGRTLALPEI
                     QQLIHDWVQEGLIMTCPHGRRTAFRLSTDELAKLFGRVGWS"
BASE COUNT         1187 a         1680 c         1392 g         1142 t
ORIGIN      
        1 aacatctgaa ctcgaacgac ggaccaagag ggacgactgt tcacggctta agaggaagat
       61 gagcgtgata atcgcgatcc cgatcgcgat cgcgacggcc tttcggttgg ctttacgctt
      121 ccgctcttct tccgcctgcc ggactctcaa ccgttcccgc tcaacatgct tgtcgtaata
      181 ggcacctgcc gattgatcga atcggtggat cgcatcgtct tcatcaagcc ccagggatcg
      241 ggcataggat cggacaaacc cgcgtgcaaa tacttgatcg ggcaacttgg cgaaattccc
      301 atcttccaac gctttcacaa aatcagttcg aatgcgcgtc ttcgatgcga cttcgtcgat
      361 cgtgagccct ttggtctctc gcacttgcct gaaaaattcg ccgatcgatt ccatcggtgt
      421 ctctctatgg cttaatccgt gtcaggagtt ccttcgcagc tgcggcatac tcaccgccct
      481 tgtcgagcgt cgtcaccttc gtcaactctt ctcttgagcg tcgttcaaac cccagcttgt
      541 aatatacgcg tcccaactcc aagtgcaact gcgccggtgg aacgttggga ggattgacca
      601 cgagggcatc ctcaaatgcc tccatcgcag cctgataatc attttcgtga gccagcaccc
      661 ggccaagatg gaatcgggcc aggtccggcg tgacatacaa gggattggtc agtgctcgac
      721 gaaatgccgc gatcgcttcc ttccaccgcc cctgtttctc caagacttgg cccagatagg
      781 tgtacgcttc cgagtaacta tcatctagtg ccgtcgcagc ccgaaattct tgctcggctt
      841 ggggcaattt accctgcagc gccagaatat ggcctagccc gtatcgtgct tctttgttgt
      901 caggatcgag cgtcacggcc ttctggaatg ccacaaacgc cttctgctga tcctgctcca
      961 ggctcgcgac gccttcctgg taatatcctt ttgacttctg tgccgaacct tctgatgtgg
     1021 cacaaccagg cagccatccg atcaacaggg ccaataccag caccagaccc acgaatggtc
     1081 gaatccacga accagcacag tgaatctgcg tcacgaaatg tcctcgaaat gagtcaactg
     1141 cttgaaggct cgaaaccgtt cttcaacttc tttgtagtcc aggattcgca atcggtcaag
     1201 actaaaggct tctacggtaa atgacgccat cacgctcccg aaaataatcg cttgcttgaa
     1261 ggcctcgacc gaacgattgc cggtcgccgt caggtagccc aggaagccgc cggcgaaggt
     1321 atcccccgca ccggttggat cgcgcacgtc ttcaagcgga aaggccggag ctccgaacac
     1381 ctgtttctcg ttgaacatca ggaccccata ctctccacgc ttgatgatga ggtgtttggg
     1441 tccacgcgcg agtacttttt gagcgacctt caccagattc gtatcttcgc caagggcccg
     1501 ggcctctcca tcgttaataa tcaaaatatc gacctgctcc aaaacccgcc ataacgcatc
     1561 tcgcttgccg ttgatccaaa agttcatcgt atcgcatgcg acaacaggcg gacgtggaag
     1621 cttctgcaac acatccaact gaagctccgg atcgatattg cccaaaaaca agagatccgg
     1681 cgatcgatag gcctcgggaa tctttggtcg aaaggtttcg aatacattga gcttcgtatc
     1741 gagcgtgcgc gcttcgttca actgatggct gtactccccc ttccatcgaa acgtcgcccc
     1801 aggcctacgc tccaatcccg tcacatcgat gccacggctc ttgaggaacg ctaggtgctg
     1861 cggagggaaa tcttccccca ctactgcaat gagatcgacg ctggtaaagt agctggctgc
     1921 agtcgaaaag aaagtggccg atcccccaag gacctcgctg acctccccga agggtgtttt
     1981 taccgtatcc aacgcgaccg acccgacaac gagcaacttt cccatcgctt agtgaatccc
     2041 cttctgtgcc ggaacaaccc gatccatcaa caaagccagt ttccgtcgga gggaagcagg
     2101 catccgatcc ggtgccgtga taatggcatg ttgcaatgcg tgctgacagc tgcaagcctt
     2161 gacatcggcc accccaggca gcgccgctcg caatagtcgc ttggccagcg tgacattctg
     2221 acggagcgtg gtcaagatcg cctcaaccgt gacggcctct tcagtctcat gccaacaatc
     2281 atagtctgtc gccaaggcca ccgtggagta acagagctct gcctcacgtg caagcttggc
     2341 ctcaggcaga ttcgtcatcc cgatcacact cgctccccac tggcgataca gccgagactc
     2401 ccctttggtc gaaaactgcg gcccttcgat gcagagatag acgccgccct gatgcacggt
     2461 cgcaccgact gtacgggccg cagccaacaa tacctcaccg agcgaggcgc agaccggatc
     2521 gccaaatgcc acatgtgcga caatccctcc ctcaaagaat gtggacaccc gccgtttggt
     2581 gaggtcgata aactgatcag gcactatcac gtccccgggt tggatcgact ccttcatact
     2641 cccgactgca ctcacagaga tcacctgtcg aaccccgagc cacttgagtg cataaatatt
     2701 tgcgcgatag ttgatttctg agggattgat ccaatggcct cgtccatgtc gggagagaaa
     2761 cgcgatgcga actccgttca actcgccgac cagtacctta tcggagggag gcccgaaggg
     2821 agtcttgacc gccagttcct tgaccttccg aaatccctcg atctcgtaga gcccgctgcc
     2881 gccgatgatc ccgatatctg cgcgcgatac gctcctctgc cgtgtcatcc tggatcacca
     2941 agtgtggcga gaaagcgatc gacctgtgcg gtcaccagcg ctgccgtgtg ctgaaccgac
     3001 tccgactcct cgatcgtcaa ccactgaatc cccggttcct ttcgaaacca cgtcatttgt
     3061 cgtttggaat aatgtctcgt gtcccgtttg aatcgccgaa ccatttcagt ggcgtcatac
     3121 tcgccggcca aatgctcagc cacctggcga taccccaggc ccttcatggc tgcgctgtcg
     3181 cgtcgatacc cccgggccag gagctgcttt gtctcttcta tcaaaccgtg ggccaattgc
     3241 caatcaatcc gttcctcgat acgccgatac agggcatcgc ggtcgcggtt caagccgatg
     3301 atcaaggctg aaaacggtcg ctcggcaaat ccatgctcct gctgatactc cgacatccga
     3361 cgaccggata cctgatgcac ttccagcgcg cggatcactt tcgattcgtc gcgggggtgc
     3421 aatcgggccg caacgacagg atcgacagcc acgagccgcg catagaggcg atcatgcccg
     3481 tggtccctga cgtcttgtcg cagagcccct cgcacgattg gatcggttgg tggtgcgtcg
     3541 cacaagcccc tgagcagagt cctcacatac agccctgtgc caccaacaac caatggaagg
     3601 cggcagcctc gatagaggcg ctcgatgtca tcgagcgcct gacgtcgata caaccctgta
     3661 ttaaatgatt cgtcaggatt gacgagatcg attagccgat gaggaacacc ctggcgctcc
     3721 tccggagtcg gcttatccgt cccgacgtcc atcccacgat agacttgccg ggagtatgcg
     3781 gtgagtacct cggtttcgaa cgcctttgcc acttcgaccg cgacgcggct cttccccaca
     3841 gcagtcgggc ctatcagcac gatgacaggc ttgcagtgga cctgcgtctc ggatagacga
     3901 cacatcgctc tcgctggaat ggccatcagg accatccaac ccgcccgaac agcttggcca
     3961 gctcatcggt cgaaagacga aacgccgtgc ggcgtccatg cgggcaggtc ataatcaacc
     4021 cttcctgcac ccaatcgtga atcaactgtt gaatctctgg aagggccaag gtacgtcccg
     4081 cacgcaccgc tccgtggcag gccaacgacg ccaataccgg ttgaacccgt agttccagag
     4141 acgatgcggt gtcccactcg atcaaatcgt cgagcagatc ctgcaccagg acagccgcat
     4201 caaccttgcc caacccgacc ggcaccccgc gaatggccac agccgtcgta ccaaacggtt
     4261 caatcaggag accgagtttc tccaaatcat cacggcgctt gagcaacagc gtgttctgcg
     4321 ccgccgacaa ctcgatcggc tccggaatta acagcggctg cgaagggata tcccgactct
     4381 gccactcccg ccacagccgt tggaaaagga cgcgctcgtg cgccgtatgc tgatcgacca
     4441 cttgcaattc gtcgcccacc tgcgccacga gataggtacg gaggatttgt ccaagcggca
     4501 cgatgcccgg cctctctgcc tgctgatagg ctgccgttgc ctcatgggcg aaggccaact
     4561 gatttccctc gacggtacca gacaggggca ccgtgtcgct caccttgttt ctcgcgtcct
     4621 gtcttagagt tgcggtcaaa ggggccgggt gaaaatatcc ggacgatgag gtctgccgtc
     4681 cagtagtggg agcgaactcc gggattgtct gtgacagccc cgcctgcgtg agacccagca
     4741 cgaccttccg ctcgggccca ccgagtgcgt gacggacaga ctgacgcact aactggtgaa
     4801 gtgcttctgt ttctgcaaaa cgaacctctt tcttcgtggg atgcacgttg acatcgagtc
     4861 ggtccggttc gagatccaga aagagcacat atgtcggatg atgccctttg ggcaaaaatg
     4921 atccataccc atccatgacc gcatgaaaca ccgtcgcatt gcggatgggg cgccgattca
     4981 caaacaattc ctgtggcgtt ctcgaggccc gcgcatgaag aggatcgacc atcacgccgc
     5041 gaatcgagca tcccgctatc cggcctcgca cctcaattgt ccgatcgaga aatgcctggc
     5101 gatagacttg aagtattcga tcacgatcag ctgtcacggc gggatagttg agaatctcct
     5161 ggccgttatg ggtcagacgg aaatgcaccg atggccaggc gagacctgcg agttgcacga
     5221 catgactgat atgggaaaac tcggtcgtgg ccgatttcag aaacttcttt ctggccggct
     5281 gattgaaaaa tagattggac acgtcgatcc gagtgccagg cacggcgggg gcatccacga
     5341 tagcgcgacc tgctccggcc accagtgtga gctgtgaacc cactgtatct tgtctcgttg
     5401 c
//
LOCUS       DATIPF010000061         3739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_627860, whole genome shotgun sequence.
ACCESSION   DATIPF010000061 DATIPF010000000
VERSION     DATIPF010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627860"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VLL06_01615"
     CDS             complement(<1..366)
                     /locus_tag="VLL06_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSS29694.1"
                     /translation="MKHNPGFLKLVDAARTRVKECTVAQAKARLDRGEVVHFLDVRED
                     HEFAKDHAKGARHLGKGIIERDAETCIPNKDEAVLLYCGGGYRSVLAADALQQMGYTN
                     VVSMDGGIKAWRDAGYPMEH"
     gene            complement(391..1404)
                     /locus_tag="VLL06_01620"
     CDS             complement(391..1404)
                     /locus_tag="VLL06_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HSS29695.1"
                     /translation="MRTVMNPPNPFDSRHRDLLEPAPHVQLQMFDDATRDILSRNESP
                     DLPFRWSLNPYRGCFHACAYCYARPTHEYWGFGAGTDFESKIIVKREAAALLRRTFEK
                     PSWNGELILFSGNTDCYQPLEASLELTRACLEVCAEYRNPVGIITKGVLVLRDLDVLC
                     RLNQEATVHVYLSIPFSDDEVARKVEPHAPSSRKRFEAMAILANAGIPTGISLSPIIP
                     GLNDEDMPDLLARAKQAGAVEAMATLLRLSGPVESVFMERMTAAFPDRITKMTNRIRD
                     VRGGVISEGAFFERHRGAGPYWAMIEQMFEISRRKAGLSAFRGEMIPTTFRRPGVQQT
                     ALF"
     gene            1541..2827
                     /gene="serS"
                     /locus_tag="VLL06_01625"
     CDS             1541..2827
                     /gene="serS"
                     /locus_tag="VLL06_01625"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HSS29696.1"
                     /translation="MYDLRTLRDNFDAIREQLGPRGTDVPWDRIRTLIEQRRTSSSQS
                     EQLRHELKKGSEDVAKLKREKQPADAVMAAMKTIGERIKTIEDELRVVEDTLTDLNLR
                     IPNVPHASVPAGKDANSNMETRRWGSPPGLTTPAKSHWDLGETLGILDFDRAAKITGA
                     RFAVLTGAGAQLERALINYMLDLHTTQHGYREVLPPFLVNRPSMTATGQLPKFEEDLF
                     RLREEDYFLIPTAEVPVTNLHRDETLTESILPIRYTAYTPCFRREAGSYGKDTRGLIR
                     LHQFNKVELVTFAKPDQSYEELERLTGHAEAIMQGLGLHYRVVTLCTGDMGFSAAKTY
                     DIEVWLPSQNHYREISSCSNFEGFQARRANIRYKGTAVKKDAKAEFVHTLNGSGLAVG
                     RTLVAILEHYQQPDGSVMIPEVLRPYMGGTGRIPKA"
BASE COUNT          875 a         1092 c          948 g          824 t
ORIGIN      
        1 gtgctccatc ggatagcctg catcccgcca ggctttaatt cccccgtcca tcgacaccac
       61 attcgtatag cccatctgct gaagcgcatc cgcggctaag accgaccgat agccacctcc
      121 gcaatacaac aaaacggcct cgtctttatt gggaatacag gtttccgcat cacgttcaat
      181 aattcctttt cccagatgac gggcaccttt cgcatgatct ttcgcaaact cgtggtcctc
      241 tctcacatcg agaaagtgta cgacctcacc ccgatcgagc ctggcctttg cctgcgccac
      301 cgtacattct ttgacccgcg ttcgggccgc atccactaac ttcagaaaac ccggattatg
      361 tttcatcccc cctcctttcc ctgtcctgtc tcaaaataac gcagtttgtt ggacccccgg
      421 cctgcgaaag gtggtgggaa tcatctcacc acggaacgca gacaatcctg ccttgcgtct
      481 ggaaatttca aacatttgct cgatcatggc ccaataggga cctgcgccgc gatggcgttc
      541 aaagaaggcc ccctcgctga tcactccacc acgcacgtcc cgtatccgat tcgtcatctt
      601 ggtaattcga tctggaaatg ctgccgtcat tcgctccatg aacaccgatt ccacagggcc
      661 agacagacgc agcagcgtcg ccatagcctc caccgccccg gcctgtttcg cccgagccaa
      721 cagatcaggc atatcctcat cgttgagacc gggaatgatc ggagacagtg agatgccggt
      781 aggaatcccg gcattcgcca agatcgccat ggcctcgaag cgtttgcggc tggaaggcgc
      841 atggggttcg accttgcgtg ccacctcatc gtctgaaaac ggtatgctga gatacacgtg
      901 aaccgttgct tcctgattca accgacacag cacgtccaga tcgcgcagca ctaatacccc
      961 tttcgtgatg atcccgaccg gattccggta ctcggcacag acttccaggc aggccctcgt
     1021 caactccagc gacgcttcca gcggctgata acaatcggta tttcctgaaa acagaatcaa
     1081 ttcgccattc catgaaggtt tctcgaacgt ccggcgaagc agagccgcgg cctcccgctt
     1141 cacaatgatc ttggattcga agtcggtccc ggcaccaaat ccccaatact catgcgtcgg
     1201 cctggcatag caataggcac acgcgtggaa gcagccgcga taggggttga ggctccatcg
     1261 aaacggcaaa tcggggctct catttcgact gagaatgtcc ctggtcgcat cgtcaaacat
     1321 ctgcagctgc acatggggag ctggttccaa gagatctcgg tgccgtgagt caaacggatt
     1381 cggtggattc atcaccgtgc gcatcggaat atggtcctcg atctgtccgc tcctgtcaat
     1441 gggacaaccc acgcctgcca cgcgggatat cacgacccaa ttgattgact aacccctacc
     1501 tcgatgttag attccagccg ccttctgaac gcacacgact atgtatgatc tccgcacgct
     1561 ccgcgacaac tttgatgcca tcagggaaca gcttggcccc cgtggcaccg atgtcccttg
     1621 ggatagaata cgcaccctga tcgaacaacg tcggacctcg agtagccagt ctgagcaact
     1681 tcgccacgaa ctgaaaaaag gatcggagga cgttgccaag ctgaaacgag agaagcagcc
     1741 cgcggatgcc gtcatggccg ccatgaagac cataggcgaa cggatcaaga cgattgaaga
     1801 cgaattacgg gtcgtcgaag ataccttaac tgatctgaat ctccgtatcc cgaatgttcc
     1861 acatgcctcg gtgccagcag gaaaagacgc gaacagcaat atggaaaccc ggcggtgggg
     1921 atctccaccc ggcctgacca caccagccaa gagtcattgg gatctcggcg aaacactcgg
     1981 cattctggat tttgaccggg cggcgaaaat caccggtgca cgattcgccg ttctgactgg
     2041 tgccggtgca caacttgaac gggccctaat caactatatg ctcgaccttc acacgactca
     2101 acatggctac cgggaggtct tacctccatt cctcgtcaat cggccaagca tgaccgcgac
     2161 aggccagtta cccaagtttg aagaggatct ctttcggctg cgtgaggaag attatttcct
     2221 gatccccacg gctgaagttc cggtgaccaa tctccaccgt gatgagacac tcaccgaaag
     2281 tatcctaccg atcaggtata cggcctatac cccctgtttt cgccgcgagg caggatccta
     2341 tggaaaagac acgagagggc tcattcggct ccaccaattc aacaaagtag agttggtgac
     2401 atttgcaaag cctgatcagt cgtatgagga actggagcgt ttgaccggtc atgccgaagc
     2461 gatcatgcaa ggattaggac ttcattaccg cgtggtcacc ctctgcaccg gcgatatggg
     2521 attctctgcg gcgaagacct acgatatcga agtctggctt ccatctcaga accactatcg
     2581 ggagatctcc tcctgcagca attttgaagg atttcaggcc aggcgggcca atattcggta
     2641 taaaggcaca gccgtgaaga aggatgcaaa ggcagagttt gtccataccc tcaatgggtc
     2701 tgggcttgcc gtaggccgca cactcgtggc catcctcgaa cactaccagc aaccggatgg
     2761 gagcgtcatg ataccggaag tgttacgtcc ctatatgggg gggactggac ggatccccaa
     2821 agcttagagc caatggccgt tggtcacctg cacttgctga gggattttga ccgaagactc
     2881 ttgaccatct gactcagtta acccgcatca acattgggga tgggctcgca atgcacctcc
     2941 ctcatgttcc tcgattttcc tatagtttcc tcgactattt cccatcacta ttcacaacgc
     3001 cattaggcgg gtgtatggat tcgtgagttt ccaatgacag gcacctctca aactaagaag
     3061 cgtcttcact aaatcactga gtcgacggtc tctgctcgag gagggagacg taaacttgtg
     3121 gcaggcctag gcaacctctt gcatgagact actcatgtct cgcggccact cgtgccgaca
     3181 gttctgattc acacaggccc aaagatccac ccgcgggatg gtcattttct ggccggagag
     3241 cgtccagttc gcggttggtt ccgcatggct cagacatttt gggcaggtca tcgtcccctc
     3301 cttctcaaag aaatgtggcg tagggtacca ctggatgaaa aggatgggac tagcaagatc
     3361 cctagttttg aaagaggcgt tgtattcgat cggcgtctca tgggcaaatt tcagcttaac
     3421 acagcccaat tgactcgcta tgccattcag catgagtccg acataaccat gcccattcct
     3481 gcgcagccgg aacctcgtcc gctgattgcg caggatgcca tatgtgcatg gtccacctgt
     3541 cacatcggag cctggagcgc gaggagtggc cggggtaagg gaatctggaa aggttgctga
     3601 atcaggctga tgcgtctctc ggccatttgt gcagacatag cggattccga caggcccaca
     3661 ggggcatggg aattccttgt ggtttgaggt gagtactggg gttgctccat acaggtttga
     3721 caccgtcgga atgacattt
//
LOCUS       DATIPF010000062         4140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1424130, whole genome shotgun sequence.
ACCESSION   DATIPF010000062 DATIPF010000000
VERSION     DATIPF010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424130"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..432
                     /locus_tag="VLL06_01630"
     CDS             64..432
                     /locus_tag="VLL06_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29697.1"
                     /translation="MAGLGRYVWSVDRAGNNVEIIDTVSNLSVGSVELVTDANKDPAP
                     DLMDNAPDGQYVFVGLRGPNPLTGNDKSVNNAVGTIPGVGVIHVDGGGKVGHYKGQAA
                     ITNMKDGKETADPHGVAVRK"
     gene            576..1415
                     /locus_tag="VLL06_01635"
     CDS             576..1415
                     /locus_tag="VLL06_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSS29698.1"
                     /translation="MTLTAYNHVSVPSFMYGTAWKKEATTQLVQSAVAAGFTAIDTAN
                     QLIHYQEDLVGEALLILAQQGIARDRLFLQTKFTPRNGQDHRTPYDASASLTTQVTQS
                     FDSSLAHLHTDYLDSYILHGPYQRQGLGNADWEVWAAIEGLYQSGKSKMIGISNVTAE
                     QLTQLCARASVKPMMVQNRCYAALGWDKTVREICRAHGIIYQGFSLLTANREIFAEPS
                     VRAIAQRLGTGLAQVVFRFAMQVGMLPLTGTTNPQHMKDDLLAEQLTLTAADMQVIET
                     IGM"
     gene            complement(1570..3108)
                     /locus_tag="VLL06_01640"
     CDS             complement(1570..3108)
                     /locus_tag="VLL06_01640"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HSS29699.1"
                     /translation="MAQATTPVVTAEINCEICALVPPPLRPLFDPQAHALPALQATCQ
                     KILNMSGDTSSPDALAQVISRDPGLTCKVLQIANGIAYSPQQMITSVTHAVTWLGLDT
                     VRTLVATAQLMEHLKQEPDRRPVLGRLIAKALFASTHAAELGAAMNYPHPGQLFSAAL
                     LYSFADLVIAYQTPDLYELLSEARTPADEAAILGISKARFATALARTWVLPTGLVDLI
                     GTPTPAVETRWQTNQQIFVGLVAGANNLVSALADSSKPNVADRIRRTLQQGTALPERN
                     LQEALTRAFDKGRQLSRSVGIMETVLAQASASVPTLAPAISPPKIQVQPPRDQPASRP
                     TVPPIQAHPFETLERFQTSLLAAKDFNSLLSILVNALHDDGGFTRVALALLNPGDTDQ
                     LLGRVVVGVGADTPTQYLSSFMGSLSADHPLFLQILKRHDSLLVSNVSAENPQSLHST
                     FVSTWHTSSAVLAPIRIGNRPIGLLYCDRGPHATQVSPHDLQSFQLFFGQAILSLNRM
                     AGVL"
     gene            complement(3283..>4140)
                     /locus_tag="VLL06_01645"
     CDS             complement(3283..>4140)
                     /locus_tag="VLL06_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC subfamily B transporter ATP-binding protein"
                     /protein_id="HSS29700.1"
                     /translation="AARVFAQIREAQGATQRVFEILDTHSEVSDSPTAMSLSTVSGHI
                     RIDHVSFAYDPRQPVLTDVSFEVKPGELVAIVGPTGAGKTTVMNLLHRFYDPTEGHIT
                     IDGQDLRQVTMDSWYRQIALVPQETILFGGTILDNIRYGDREATEETVVAASRAAHAH
                     DFIMSFPDRYQTIVGEKGINVSGGQRQRIAIARAIVKNPRILLLDEATSALDSESERL
                     VQEALEQLMKGRTTFVVAHRLTTIQRADRILVLNKGLLVETGTHAELMDRKGLYQYLY
                     TLRLIELPS"
BASE COUNT          849 a         1102 c         1304 g          885 t
ORIGIN      
        1 ccgaaatcgg tctcggccaa gctggtgact taacgggatg ataaattcgc cgattcccat
       61 gggatggccg ggctcggacg ctatgtctgg agtgtggatc gggccgggaa caacgtcgaa
      121 atcatcgaca cggtgtcgaa tctgagcgtg ggctcggtcg agttggtgac ggatgcaaat
      181 aaagatccgg ctcccgatct catggacaac gcgccggacg gtcagtacgt cttcgtcggg
      241 ctgcgcggcc ccaatccgct caccgggaat gacaagtcgg taaataatgc cgttgggacg
      301 atccccggcg tcggtgtcat tcacgtggat ggcggaggga aggtcggtca ctataagggg
      361 caggcggcca tcacgaatat gaaagatgga aaagaaacgg cggatcctca tggggtcgcc
      421 gtccggaagt agcaccgctc ttgggcaata tcgggggccg cggcctgctg ccgcggtcca
      481 cgaggtgtgg atgagagatt gtggttgcct cttcgtgatt ggtcgctgcg tcctcccgaa
      541 cgcgtcagcc gtccgttcat cttggagaat agcggatgac cttgaccgcc tataaccatg
      601 tgtcggttcc ttcgttcatg tatggtacgg cttggaaaaa ggaggccacg acacaactgg
      661 tgcagtcagc cgtggcagcg ggcttcaccg ccatcgacac ggccaaccaa ctgatccatt
      721 accaggaaga tttggtaggg gaagctctgt tgatccttgc gcagcaaggg atcgcgcggg
      781 atcgcctctt tcttcaaacc aagtttacgc cgaggaatgg ccaggaccat cgtaccccct
      841 atgatgcctc ggcatctctc acgacccaag tgactcagtc cttcgacagt tcattggccc
      901 atctgcacac cgactatctc gactcctaca tcttgcacgg gccgtatcag cggcaggggt
      961 tgggaaatgc ggactgggag gtgtgggctg ccatcgaagg gctgtatcag tcggggaaat
     1021 cgaaaatgat cggcatcagc aatgtgacgg ctgagcaact cactcagctc tgtgcccggg
     1081 cgtcggtgaa acccatgatg gtacaaaatc gatgttatgc cgcactggga tgggacaaaa
     1141 cggtgcggga gatctgccgg gcccacggca tcatctacca aggcttttcg ctgttgactg
     1201 ccaatcggga aatctttgcc gagccaagcg ttcgggccat tgcccaacgg ttagggaccg
     1261 gcctcgcaca agttgtgttt cggtttgcga tgcaagtcgg gatgctcccg ttgaccggca
     1321 cgacgaatcc gcaacatatg aaagacgatc tcctggcgga acagctcacc ctcactgctg
     1381 cagatatgca ggtcatcgaa acgatcggga tgtaaacgcg acacctcggt gctcgtgacg
     1441 cgtagttcgt cgtgcaacat ccagtgtatt gcttcctgtg tgcctcacat tatacggatg
     1501 aggcgtcact caccttctta ttccccactc tgttgaatca gcttctccac gaccgtgccc
     1561 ggcgcatcct cagaggactc cggccatccg attgaggctc agaattgctt ggccaaaaaa
     1621 cagctgaaac gactgtaggt cgtggggtga tacctgggtg gcatgcgggc cacggtcgca
     1681 gtagaggagt ccgatcggtc ggttgccgat gcgaatgggg gccaggacgg cagacgacgt
     1741 gtgccaggtc gagacgaacg tcgagtgaag ggattgagga ttctcggctg agacattcga
     1801 gaccaatagt gaatcatgcc gtttgagtat ctgtaagaat agcgggtggt ccgcgctgag
     1861 cgatcccatg aagctggata ggtactgagt tggcgtgtct gcaccgacac cgacgacgac
     1921 tcggccgagc aattggtcgg tatcgccggg attcaagagc gccagtgcca cccgtgtgaa
     1981 tccgccatca tcatgcagtg cgttgaccag gatcgacagc agactattga agtcttttgc
     2041 cgcgagcaac gaagtctgaa acctttccag agtttcgaac ggatgtgctt ggatcggcgg
     2101 aactgtcgga cgactggctg gctgatcacg aggtggctga acctggatct ttggtggcga
     2161 gatggcggga gccagtgtgg gaaccgaggc tgacgcctgg gcgaggacgg tctccataat
     2221 cccgacggaa cgggagagtt gacggccctt gtcgaaggcg cgggttaagg cctcctgcaa
     2281 gttgcgctca ggcagagcgg tgccttgctg gagggtgcga cgaatccggt cggccacgtt
     2341 gggcttcgat gaatcagcca aagcagaaac gagattgttc gcccccgcga ccagtcccac
     2401 aaatatctgc tgattggtct gccaccgggt ctccacggcg ggcgttggcg taccgatgag
     2461 gtccaccaag ccggtcggca gaacccatgt ccgcgccaag gccgtcgcaa agcgggcttt
     2521 cgaaataccg agtatggcgg cttcgtcggc cggtgtccgc gcttcggaga gtaattcata
     2581 gagatccggc gtttggtagg cgataaccag gtcggcgaac gagtacaaca gcgcggcgct
     2641 gaacagctgt cccgggtgag gataattcat cgcggcgccg agttcggccg cgtgtgtcga
     2701 tgcgaagagc gcctttgcga tcagtcggcc caggaccggg cggcgatccg gttcttgctt
     2761 caagtgctcc atcaattggg ctgtggcgac gagcgtgcgg acggtatcca gcccgagcca
     2821 ggtgaccgca tgagtcacgg aggtgatcat ctgctgtggg ctgtaggcaa ttccattggc
     2881 gatctggagc actttgcagg tcaacccggg atcgcgactg atgacttgcg cgagcgcgtc
     2941 ggggctcgat gtgtcgccgc tcatgttaag aattttctga caggtggcct gcaaggccgg
     3001 gagcgcatgg gcctgggggt caaagagcgg gcgtagcggc ggaggcacca gtgcgcaaat
     3061 ctcgcaattg atctcagccg tcacgaccgg ggtagtggct tgcgccattc tgcctcctga
     3121 atagtgtgtg gacaggccgc tgaaacagac ggtcggtttt caaggtttag agcgctagta
     3181 gtcggaaaat ctccgttgtc tctctatcgg tcgattgaag gttgaaactt agccgcttcg
     3241 cgagggttcc cggcgttcga cggctggtgt ggatgtcaag acttacgagg ggagttcgat
     3301 gagccgcaga gtatagaggt actggtacag ccctttacgg tccatcagtt cggcatgggt
     3361 accggtttcc acgaggagcc ctttgttcag gacgaggatg cgatctgctc gttggatggt
     3421 cgtcaagcgg tgggcgacca caaacgtcgt ccgccctttc atgagttgtt ccagcgcttc
     3481 ttgaacgagt cgttccgact cactgtcgag ggcggaagtg gcttcatcca acaataagat
     3541 tcgtggattt ttcacaatag ccctggcgat ggcgatgcgc tgacgctgtc cgcccgagac
     3601 attgatccct ttttctccca caatcgtctg ataccggtcc gggaagctca tgatgaagtc
     3661 gtgggcatgg gccgcacggc tcgccgcgac caccgtttcc tcagtggcct ctctatctcc
     3721 atagcgaata ttgtcaagaa tcgttccgcc gaataggatc gtctcttgcg ggaccagcgc
     3781 gatctgtcga taccaactgt ccatggtgac ctggcggaga tcttgcccgt cgatggtgat
     3841 gtgcccttca gtgggatcgt agaagcgatg aagcaggttc atcacggtgg tctttccagc
     3901 gccggtgggc ccgacaatgg cgacgagttc gccgggtttg acttcgaacg atacatccgt
     3961 caagaccggt tggcgtggat cataggcaaa gctgacatga tctatccgga tgtgtcccga
     4021 gacggtggac agcgacatgg ccgtggggga gtcgctcact tctgagtgtg tgtcaagaat
     4081 ttcaaatacc cgttgggtgg caccctgcgc ctccctgatc tgtgcgaaca cgcgggcagc
//
LOCUS       DATIPF010000063         2004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_628496, whole genome shotgun sequence.
ACCESSION   DATIPF010000063 DATIPF010000000
VERSION     DATIPF010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628496"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..173
                     /locus_tag="VLL06_01650"
     CDS             12..173
                     /locus_tag="VLL06_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29701.1"
                     /translation="MAGGLTPDNVGKAIHAVQPYGVDVSSGVERAPGKKDHEKVRAFI
                     RAVRVVSLT"
     gene            238..1431
                     /gene="trpB"
                     /locus_tag="VLL06_01655"
     CDS             238..1431
                     /gene="trpB"
                     /locus_tag="VLL06_01655"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HSS29702.1"
                     /translation="MSSVPNKQGRFGAYGGRYVPETLMPALLELEQEYARVKKDRSFQ
                     SELAQYLKHYVGRPTSLYFAQRLTKRLGGAKIYLKREDLCHTGAHKINNAIGQGLLAK
                     RMKKPRIIAETGAGQHGVATATVAALFGLQCEIYMGTEDMERQALNVFRMRLLGSTVT
                     GVDAGSRTLKDAISEAMRDWTTNIRTTHYILGSVLGAHPYPMMIRDFQSIIGRETRRQ
                     ILAEEGRLPNCLIACVGGGSNAIGLFHAFIQDKKVKMIGVEAGGLGVASGKHAARFAG
                     GTPGVLQGTMTYLLQDADGQINLTHSVSAGLDYAAVGPEHSYLRDQGRAEYTSVTDDE
                     AMAAFDLLTNEEGIMPALESAHAIAHTMKVAPTMKKSQILVVNLSGRGDKDVHIVAKM
                     RGITL"
     gene            1428..>2004
                     /gene="trpA"
                     /locus_tag="VLL06_01660"
     CDS             1428..>2004
                     /gene="trpA"
                     /locus_tag="VLL06_01660"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HSS29703.1"
                     /translation="MTSRLDRTFARLRQDGEQAIIAYVMAGDPSLQETEHLVVELERA
                     GADIIELGVPFSDPIADGPVIQQAAARALRSGTSVRTILPMVARVRTMTQIPLVLMIY
                     YNNIHAFGPERFCHEAAQAGVDGLIVPDMPPDEAGPLRRPAAAAGLQLIFLLAPTSTA
                     ERRTFVARQSEGFVYCVSLTGITGAKLHNLAD"
BASE COUNT          448 a          531 c          599 g          426 t
ORIGIN      
        1 cgagcattct tctggccggg gggcttaccc cggacaatgt tgggaaggcg atccatgcag
       61 tacagccgta cggcgtggat gtgagcagcg gcgtcgagcg ggcaccgggc aagaaggatc
      121 acgagaaagt gcgtgcattc attcgagccg tcagagttgt ctctctgaca tgaaccccct
      181 tatactgcat ccggcgacgg atgttctgca gcctgttaca cgaatgaggc ggtggcaatg
      241 agttcggttc ccaacaagca aggtcgattc ggcgcgtatg gcggacgcta tgtccctgaa
      301 acactcatgc ccgcattact tgaattggag caggaatatg cccgtgtgaa aaaagaccga
      361 agtttccagt ccgaacttgc ccagtatcta aaacactacg tcggcagacc cacctcgctc
      421 tactttgcac aacgactcac gaagcgtttg ggtggcgcga agatttattt gaagcgcgag
      481 gacctctgtc ataccggagc tcataaaatc aataatgcga tcgggcaagg gcttctggcc
      541 aaacgaatga agaagccgcg catcatcgcg gaaactggag ccggacaaca tggagtggca
      601 acggcaaccg tcgcggcctt gtttggcctt caatgtgaaa tttatatggg gactgaagac
      661 atggagcgcc aggcgctgaa cgtcttccgg atgagattgc tcggctcgac cgttacgggt
      721 gtcgatgccg gaagtcggac actgaaggat gccatcagcg aagccatgcg ggattggacc
      781 acgaatatcc gtacgaccca ctacattctc ggttcagttc tgggggccca tccctatccg
      841 atgatgatcc gtgattttca atcgatcatt ggacgtgaaa ctcggcgaca gatcctggcg
      901 gaagaaggcc ggctgccgaa ttgcctcatt gcctgcgtcg gtggcgggag caatgccatc
      961 ggactcttcc atgcgttcat ccaggacaag aaggtgaaga tgatcggtgt tgaagcgggc
     1021 ggtctgggtg tcgcgagtgg aaaacatgcg gcgcgatttg ccggaggaac gccgggtgtg
     1081 ttgcaaggga ccatgaccta tctccttcag gatgccgatg ggcaaattaa tctgacgcat
     1141 tcggtttcag ctgggttgga ttacgccgcg gtcggtcccg agcatagtta tttacgggat
     1201 caaggccgag ctgagtacac ctcggtgacc gatgacgaag ccatggccgc attcgatctg
     1261 ctgaccaacg aagaggggat catgccggct ctcgaaagcg cccatgccat tgcccacacc
     1321 atgaaggtgg cgcccacgat gaaaaaaagc cagattctgg tcgtgaatct atccggtcgt
     1381 ggggataaag acgttcacat cgtggcgaag atgcggggga tcacactgtg acatcgaggc
     1441 tcgaccgcac ctttgcccgg cttcggcagg acggtgagca ggccattatc gcctatgtga
     1501 tggcagggga tccctcactg caagagactg agcaccttgt cgtcgagttg gaacgggccg
     1561 gagccgatat cattgagctg ggtgtcccgt tttccgatcc gattgccgat ggccccgtga
     1621 tccaacaggc ggcagcacga gccttgcgca gcggcacgtc ggttcgaacg atccttccca
     1681 tggtcgcacg cgtaaggacc atgactcaga ttcccctcgt cttaatgatt tactataata
     1741 atattcacgc gtttggtcct gaacgatttt gtcatgaggc ggctcaggcc ggtgttgatg
     1801 gcctgattgt gcccgacatg ccgccggatg aagcaggccc tctcagaagg ccggccgctg
     1861 cagccgggct acaactgatt tttcttctcg cccctacgag cacggcggaa cgacggacgt
     1921 ttgtggcacg tcaatcggag ggcttcgtct attgtgtgtc actgaccggg atcaccggcg
     1981 cgaaactaca caatctggct gatg
//
LOCUS       DATIPF010000064         3374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1336312, whole genome shotgun sequence.
ACCESSION   DATIPF010000064 DATIPF010000000
VERSION     DATIPF010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336312"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            353..655
                     /gene="ureA"
                     /locus_tag="VLL06_01665"
     CDS             353..655
                     /gene="ureA"
                     /locus_tag="VLL06_01665"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HSS29704.1"
                     /translation="MHLTPREQEKLLIYVAANLAKERKARGLKLNHPEAVAFVTAAIL
                     EGIRDGRSVSNLMSYGATLLKKKDVMPGVAEMIPELQVEGTFPDGTKLVTVHEPIR"
     gene            664..1128
                     /locus_tag="VLL06_01670"
     CDS             664..1128
                     /locus_tag="VLL06_01670"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF009682.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HSS29705.1"
                     /translation="MKKKTKAKQKVTTRKGVSRNAKKLSTQPATIIPGEIILGEGDIV
                     AFKDRQTVEVLVANTGDRPIQVGSHCHFFEANRALRFDREKAYGFRLQVPAGTAVRFE
                     PGEDKRVILVSISGNRVAYGINGLTHGRLDDPHVKAKALALARQQGFISKNS"
     gene            1288..3015
                     /gene="ureC"
                     /locus_tag="VLL06_01675"
     CDS             1288..3015
                     /gene="ureC"
                     /locus_tag="VLL06_01675"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF009686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HSS29706.1"
                     /translation="MKIPRRQYADLYGPTAGHRIRLAYTDLLIEITRDLTVSGEEAKF
                     GGGKVIRDGMGQSPAATRAKGALDLVITNAIIIDWWGIVKADIGIRDGRIVGIGKAGN
                     PDLMEGVTKWMEIGACTEVLSAEGKIVTAGGIDTHIHFICPQIINEALANGLTTLIGG
                     GTGPATGTNATTCTPGAWNIHRMLEAAEGFPINLGFLGKGNSSLPDGLREQVEAGAIG
                     LKLHEDWGTTPAAISTCLDVAEEYDVQVAIHTDTLNEAGFVEDTIKAFKGRTIHSFHT
                     EGAGGGHAPDIIKVCGEANVLPSSTNPTMPFTVNTMDEHLDMLMVCHHLNPRVPEDIA
                     FAESRIRRETIAAEDILHDMGAISIMSSDSQAMGRVGEVIIRTWQTAHKMKVQRGHLS
                     QTAADKLAGSNDNFRVRRYVSKYTINPAIAHGIGHEVGSVEVGKLADLVLWKPAFFGV
                     KPEIVLKGGFPMSAAMGDPNASIPTPQPVFTRPMFGSFGRALFSTSLTFLSKSAIDKE
                     LHRSLGLQKRVAAVKKCRGIGKRDMKLNDALPRIEVDPETYVVKADGQILTCDPMAVL
                     PMAQRYFLF"
     gene            3161..>3374
                     /locus_tag="VLL06_01680"
     CDS             3161..>3374
                     /locus_tag="VLL06_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29707.1"
                     /translation="MNTLALLKGFRFVDSFFPSGGYAFSSGLEAAVQGGVVRNAEELS
                     RFVVESLTTRMGEREAVVVGLAHDSFV"
BASE COUNT          748 a          860 c         1052 g          714 t
ORIGIN      
        1 ggtggcggcg ggcaagagtg atgagttgag tgcagatatg gtaaagcagc atttgacggt
       61 gtgatgtgag gtcagggggg cgggagtgct ctcctgctcg cgcaacgcgc ggtcgcggac
      121 tcccctcgtt gaacgcgcgc aatcgaaagc actcccgccc cctgaccctt cgtatcttgc
      181 ccaccgtctg ggggaacaaa gggaggagag ggggtggtcg ggatgacttc tatgctcgca
      241 gagcgcgcaa ttgaagtcat cccaaccacc cgttactatt ctcgccgctg cccagatagg
      301 agagcgaggg ttttcttatc gattgacgaa tgaccattga cggggttaca cgatgcattt
      361 gacaccgaga gaacaagaga agctgctgat ctatgttgcg gcgaatttgg ccaaggaacg
      421 caaagcgcgc ggactgaagc tgaatcatcc tgaagccgtc gcatttgtga ctgccgcgat
      481 tttggaaggc attcgtgacg ggcgttcggt gtcgaacctc atgagctacg gcgcgacact
      541 gctcaagaag aaagatgtga tgcccggcgt ggcggagatg attccggagt tacaggtcga
      601 gggcacattt ccggatggga caaagctggt gacggttcat gaaccgattc gataggtgaa
      661 tcgatgaaaa agaaaaccaa agccaagcag aaagtgacga cgcgaaaggg agtgagtcgt
      721 aatgccaaga aactttcgac tcagcctgcg acaataattc ccggtgaaat catcctcggt
      781 gaaggcgaca ttgtggcgtt caaagaccgt cagacagttg aggttctcgt ggccaatacc
      841 ggtgatcggc cgattcaggt cgggtctcat tgtcatttct tcgaggcaaa tcgcgcgctg
      901 cggtttgatc gtgaaaaggc ctacgggttt cgcttgcagg ttccggctgg aaccgcagtg
      961 cggtttgagc cgggcgaaga caagcgggtg atactggtct cgatcagcgg caaccgcgtg
     1021 gcctatggga ttaatgggct cacacatggg cggctcgacg atcctcacgt caaagccaag
     1081 gcgctcgcac ttgcgcgcca gcaggggttc atatcgaaga atagttaagt tgattaggat
     1141 gtgaaacgaa aggagagagg cagctcaaaa cggtttccag caaggccgca ggaatagtca
     1201 aagccggaag cgtaccctct ggggtacgtt gaggactttg acgagccgag aacgaagctg
     1261 gagaccgttt tcagctgccg atgagccatg aagattcctc gacgccaata tgccgacctg
     1321 tacggcccga cggcgggtca tcgtattcgc ttggcctata cggatttgtt gatcgagatc
     1381 acgcgtgacc tgacggtatc tggtgaagag gcgaagttcg gcggcggcaa ggtcatccgc
     1441 gacggcatgg ggcagtcgcc ggccgcgaca cgcgcgaagg gcgcgctcga tctcgtgatc
     1501 acgaacgcga tcattatcga ttggtggggc atcgtgaagg ccgacatcgg catccgggat
     1561 ggccgcattg tcggcatcgg caaggccggc aatcctgatc tgatggaagg tgtcacaaaa
     1621 tggatggaga tcggcgcctg taccgaagtg ctctcggcgg aaggcaagat cgtcacggct
     1681 gggggcatcg atacgcacat tcatttcatc tgcccgcaga tcatcaatga ggccctggcg
     1741 aatgggctca cgacgttgat cggcggtggg accggtccgg caactggaac caacgccacg
     1801 acctgcaccc caggtgcatg gaacatccat cgcatgctgg aagccgcgga gggatttccg
     1861 attaatctcg gttttcttgg gaagggtaac tcgtcgctgc cggatggact aagggaacag
     1921 gtcgaggccg gcgcgatcgg gctcaagctg cacgaagact ggggcacgac accggcggcg
     1981 atttcgacgt gtctcgatgt tgcagaagaa tatgatgtgc aggtcgccat tcataccgac
     2041 acgttgaatg aagccgggtt tgtggaagat accatcaagg cctttaaggg tcgaacgatt
     2101 cattcgtttc acacggaagg cgccggcggc gggcatgctc ccgatatcat caaggtctgc
     2161 ggtgaggcca atgtgttgcc ctcttccacg aatcccacga tgcccttcac ggtcaatacg
     2221 atggatgagc acttggatat gcttatggtc tgtcaccatt taaaccctcg tgtgccggag
     2281 gacatcgcct ttgccgaatc gcgtattcgt cgtgagacca ttgcggcgga ggacattttg
     2341 catgacatgg gggcgatcag catcatgtcg tccgactcgc aagcgatggg gcgggtcggt
     2401 gaggtgatca ttcgcacgtg gcagacggcg cataagatga aagtgcaacg cggacatctc
     2461 tcccaaactg cggccgacaa gctggctggt tccaacgata actttcgtgt gcggcgctat
     2521 gtctcaaaat acacgattaa cccggcaatc gcgcatggca tcgggcacga agtgggctca
     2581 gtcgaagtcg gaaagctggc ggatctcgtg ctgtggaaac cggctttctt tggtgtgaag
     2641 ccggagatcg tcctaaaggg tggcttcccc atgtccgctg ccatgggaga tcccaacgca
     2701 tcgattccca caccgcagcc ggtcttcacc agacccatgt tcggaagctt tggccgagcc
     2761 ctattttcca ccagcctgac gtttctctcc aagtccgcca tagacaaaga gttgcacaga
     2821 tcactcggcc tgcaaaaacg cgtcgcggcg gtgaagaagt gccgcgggat tggcaagcgc
     2881 gatatgaagt tgaacgatgc gttaccgcgc attgaggtgg atccggaaac ctatgtggtg
     2941 aaggccgacg ggcaaatctt aacctgtgat ccgatggcgg tgctgcccat ggcccaacgg
     3001 tatttccttt tctagcaggc tgatcaaaat gccctccagc ggcgttctcc gtcgttcgaa
     3061 cccctcaacg taccgcttcg ggtacgcctc gggattcgaa ctccgtgcgg ccttgctgga
     3121 ggccattttg atcagcctgt cggtaaggcg cgaataaact atgaataccc tagctctctt
     3181 gaagggtttt cgattcgtcg atagcttctt tccttcggga gggtatgcct tctcgtcggg
     3241 acttgaggcg gcggtgcagg ggggtgtggt tcggaatgcg gaggaactgt cgcgtttcgt
     3301 ggttgagtcg ctcacaacca gaatggggga acgggaagct gtcgtggttg gactggcaca
     3361 tgattctttc gtct
//
LOCUS       DATIPF010000065         2775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1160359, whole genome shotgun sequence.
ACCESSION   DATIPF010000065 DATIPF010000000
VERSION     DATIPF010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160359"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VLL06_01685"
     CDS             complement(<1..654)
                     /locus_tag="VLL06_01685"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSS29708.1"
                     /translation="MKELVSSATTRDTAGVERLTRSGDQVGLRDWELAEIRRSAAEAQ
                     QADRSSLRANEDNLLKYVNPPPDTLFHLEYAFHLLGNVRNQEVLEYGCGWGENTILLS
                     KRGAHVHALDISPELIAINQQRMDINGVTNGVRLSVASAYEVPCPDESVDVVFGMMIL
                     HHLDLAQAAREVYRVLRVGGRAIFSEPVRSSPTLWALRKLLPFPLPPNLSPYERPLTL
                     "
     gene            complement(675..1454)
                     /locus_tag="VLL06_01690"
     CDS             complement(675..1454)
                     /locus_tag="VLL06_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29709.1"
                     /translation="MKNARRVFVDGNLFEYNWQHPLPGYGYAITFTVRNENGGSPWAV
                     IEDVAFTNNIVRHSPNGINILGFDDIHPPSGQAKRILIQNNLFDDINGPRWGGKGRLF
                     QLLRGTKDIVIAHNTAFHTGHILMADGDPHEGFVYRSNITPHNEYGIEGAGTGVGIGT
                     LERHFPGAVVIQNVIIGGDPQRYPNGNFFPQSVQTVPFVNWSNGDYKLIPSGPASQEI
                     PGVDVKTLCAAIGIAARHENICVWPPLQESQDKRQITGMHH"
     gene            complement(1469..2260)
                     /locus_tag="VLL06_01695"
     CDS             complement(1469..2260)
                     /locus_tag="VLL06_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29710.1"
                     /translation="MDTLPKLPKEFVDVTLVHSTGRTWSIPSGGDFQDALDQAQLGDE
                     IVLEAGAVYKGPFTLPKKKQGSGWITVRSSAVGHDFPSPGSRVNPSHAAKMPKLVSSR
                     YGVIIAHKGAHHFRFIGIEVRPAENTFLRDLVMLGDNTDQSIEELPHHIILDRCFVHG
                     DPLKGSRRGLAMNGRSLAVIDSYFSDFKEVGQDSQAVAGWAGAGRSKWSITSWREPGK
                     TFSLVGQIPRFAISYHPTSKFEGITLANRFPGNQASRSMTAATGR"
     gene            complement(2353..>2775)
                     /locus_tag="VLL06_01700"
     CDS             complement(2353..>2775)
                     /locus_tag="VLL06_01700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSS29711.1"
                     /translation="FALVGKAQTDLSMLAGLSNVHLLGQQPYSSLPAYCRGFDVGVIP
                     FRMNELTLRVNPLKLREYLAAGLPVVSTPLPEVVRYNGLVHIAVNKSGFLEAIDLALK
                     ERSPENDRRRVEAMRSENWESRVSEMCDVIQSRLAEVP"
BASE COUNT          625 a          795 c          722 g          633 t
ORIGIN      
        1 aagggttaac ggccgttcgt acggcgacaa attcggggga agtgggaatg gaaggagttt
       61 ccgcaaggcc cataaggttg gtgagctacg cacgggctcg ctaaagatcg cccttcctcc
      121 aacgcgaagt actcgataca cttcccgggc cgcctgggcc aagtcgagat ggtgcagaat
      181 catcatgccg aacaccacat ctacggattc atcgggacag ggcacttcat aggcagaggc
      241 cacactgagg cgtacgccgt tcgttacccc gttgatgtcc atccgctgtt ggttgatggc
      301 aataagctcg ggtgagatat caagggcatg gacatgcgcg cctcgtttgc tgagcaggat
      361 cgtgttttcg ccccaaccac atccatactc cagcacttct tgatttctga cattgcctag
      421 cagatgaaac gcatactcca ggtggaacag tgtatccggt ggcggattta catatttcaa
      481 caggttgtcc tcgttcgcac gcaaagacga tcgatcggct tgttgggctt ctgcggcgct
      541 acgccgtatc tccgccaatt cccaatcgcg taacccgacc tgatctccag accgagtgag
      601 acgctcaacc cccgcagtgt ctcgggtggt cgcggatgag acaagttcct tcatacgtcg
      661 tctcctgttt tctttcagtg atgcatgccg gttatttgcc gcttgtcctg tgattcctgt
      721 aggggcggcc agacgcagat attctcatgg cgagctgcga tcccgatcgc cgcacagaga
      781 gtcttgacat caacaccagg gatttcttga gaagccgggc cagatgggat caatttataa
      841 tcgccattcg accagttcac gaatgggact gtctgcaccg attgggggaa gaaattgccg
      901 ttcggatagc gctgtggatc cccaccgatt atgacattct gtatcaccac ggcacccggg
      961 aaatgccgct ccaacgttcc gattccaaca ccggtgccgg ctccttctat gccgtattcg
     1021 ttgtgcgggg taatgttgga ccgatagacg aaaccttcat gcgggtctcc atctgccatc
     1081 agaatatgcc cggtatggaa ggcggtgttg tgcgcgatca caatatcctt cgtgccccgc
     1141 aacagctgaa acaaccgccc tttccctccc caccgaggtc cattgatatc gtcgaacaga
     1201 ttgttctgaa tcagtatccg tttggcttgc ccgctgggcg gatgaatgtc gtcgaatccc
     1261 aagatgttaa tcccatttgg actgtgacga acgatgttat tcgtgaacgc gacatcttcg
     1321 atcacggccc atggcgatcc tccgttctcg ttgcgcacgg taaacgtgat ggcgtatcca
     1381 tatccgggaa gcggatgctg ccagttatat tcgaacaggt ttccatccac gaatacccgg
     1441 cgcgcattct tcaactcgaa taaaatcttt acctgccagt tgctgccgtc atgctccggc
     1501 tcgcctggtt tccaggaaag cggtttgcta aggtgattcc ctcgaatttc gatgtcggat
     1561 ggtacgagat cgcgaatctt gggatctgcc ccaccaaaga gaaggttttc cccggctccc
     1621 tccaagaagt tattgaccac tttgaacggc ccgctcccgc ccatccggct accgcctgtg
     1681 aatcctggcc cacttcttta aaatctgaaa aatatgaatc aatgacggcg agagaccgac
     1741 cattcatggc gagtccacgc cgcgagcctt tcagcggatc cccatgcacg aaacagcggt
     1801 cgagaatgat atggtgcggc aactcttcaa tggattgatc cgtattgtcg ccaagcataa
     1861 cgaggtcccg aagaaatgta ttctctgctg gtctcacttc aatcccgata aagcggaagt
     1921 gatgcgcacc cttatgcgca ataatgacac cgtatcgact ggacacgagc ttgggcattt
     1981 tggcggcatg tgatggattc acgcgtgatc ccggcgaggg aaagtcatgg cctaccgcgc
     2041 tggatcggac ggtaatccat cctgatccct gtttcttctt ggggagagtg aacggacctt
     2101 tgtatacggc gccagcctcc aacacaatct catcgccaag ttgtgcctga tcgagcgcat
     2161 cctgaaaatc gccaccggat gggatagacc atgtgcgacc cgtcgaatgg acgagggtga
     2221 cgtccacgaa ctccttgggc aatttcggga gggtatccat aaccggcctc tcgtcctgca
     2281 ttgccgccaa aacccacgat gaaccgacgg gtagccatag caagacccac atcacaatga
     2341 tcgaaggcag tctcatggga cctccgcaag ccgcgattga atgacgtcgc acatctccga
     2401 tacccgagat tcccaatttt cgcttcgcat cgcttccacg cgcctgcgat cgttctcagg
     2461 cgatcgttcc ttcagcgcca agtcaatggc ctctaaaaat cctgacttat tgaccgcaat
     2521 gtgcacgaga ccgttatatc gtacgacctc cggtaacggc gtcgacacca ctggcaggcc
     2581 agctgcgaga tactcgcgca acttaagcgg attgactcgc agggtgagtt cgttcatgcg
     2641 aaacgggatc acgccgacat cgaatcctcg gcaataggca ggaagcgacg agtacggttg
     2701 ctgccccaag agatgcacgt tcgagaggcc cgccagcatg gacagatccg tctgcgcttt
     2761 ccccaccagg gcgaa
//
LOCUS       DATIPF010000066         3281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_364303, whole genome shotgun sequence.
ACCESSION   DATIPF010000066 DATIPF010000000
VERSION     DATIPF010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364303"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VLL06_01705"
     CDS             complement(<1..377)
                     /locus_tag="VLL06_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HSS29712.1"
                     /translation="MFLSRRQFLKVTAGTVAAVAVADRVLALTALQPVIEVGNPLGDY
                     PDRSWERVYQDQYRYDSSFTFVCSPNDTHACRVRGFVRNGVVMRVEQNYDHQTYEDLY
                     GNRGTFAHNPRMCLKGFTFHRRV"
     gene            complement(897..2369)
                     /locus_tag="VLL06_01710"
     CDS             complement(897..2369)
                     /locus_tag="VLL06_01710"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSS29713.1"
                     /translation="MITHGGVTDMRSTRTKSRAPFTILIVEDNATDVELMLHALEAAD
                     LKPLGGDFEMEVRASAEGALQLLGERSVDLVLTDMVLPGMDGLDLVSRIQGIDSNLPV
                     LVYTWTNAVPLAVDAMRRGAFDYVLKPVNAEDLGMRLHRAIRISEILRRHAVYEHRDR
                     QEFEANSFVGSGISFQHIMRSIQEAAQVRSTVLITGETGTGKGMIARAIHQQSPERDR
                     PFQVIDCTTVPEGMMESELFGHVRGAFTGAIADKPGLIELANGGSVFFDEIGELPLPL
                     QAKLLRVLEESEVRPVGGTRVRRVDMRFIAATNRNLEARVREGTFRKDLYYRLAVISI
                     PVPPLRERREDIPVIARHLLSRLFQSMGKAQCHFDEEAVRALVSYSWPGNGRELRNVV
                     ERMVMLATGDIIRGRDVRGMLPKNEAESLASVDPTLTFLPYMEAKERALEEFTKSYLR
                     VKLAQHGGVITKAAEESGIPRQHFSLLMKRFLGRDGVENQ"
     gene            complement(2380..>3281)
                     /locus_tag="VLL06_01715"
     CDS             complement(2380..>3281)
                     /locus_tag="VLL06_01715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS29714.1"
                     /translation="SLLAERPRGAQEWNLRLAEGLAQEAALAISNARLYEAAAQRQRG
                     LAARLQQLEHLAETLAHDLKGPGARMGELARLLVQENGRQFDARTNRWLSLIEENGRD
                     LVQRVEGILTVARVGADQGAITAVDPTAIIDQVLKAKAGEIERLRAVVHIEPGLPLVA
                     CHGAYLRQIFDNLVSNALKFTRAGESPVVRISGRVEGPLVVFSVEDRGIGIPLTQRNR
                     VFQPFVRLLMSEAAGSGIGLTIIQRIVELYGGKVWIDGAEGPGCTVLFTVPSFEEHEG
                     TSNVRMQESRLPGVVDVVPRGVL"
BASE COUNT          790 a          983 c          811 g          697 t
ORIGIN      
        1 tagacgcggc gatgaaaggt aaagcccttc aagcacatcc gcggattgtg cgcaaaggtc
       61 ccgcggttgc cgtagaggtc ttcataagtc tggtgatcgt agttctgttc gacccgcatc
      121 accacaccat tacggacaaa gccgcggacc cggcaggcgt gcgtgtcgtt cggagaacag
      181 acaaatgtaa aagacgaatc gtagcgatac tggtcctgat agacccgctc ccaggaccgg
      241 tctggatagt cgcccaacgg attgccgact tcgatgaccg gctgcaacgc cgtcaacgcc
      301 aagacccggt ctgccaccgc gaccgcagcg accgtcccgg ccgtgacttt taaaaactgc
      361 ctccgtgaca aaaacatggg tcccacctcc tcacagttga gggctttacg caagcccacg
      421 cgacgtctct ccccctaaca tcaaaaacat tgcccccgct tacttcttga gagtcctgcc
      481 gtaatttcat gagtatgcag accatcaccc catttttacg cactggtcat agcaagagag
      541 ttgccaaatt gagaggccgg tgacatcaac agggtcatat tcgttaacac attgaaataa
      601 aaagtaaact actagaattt ttaaaggatg ttgaggaagg aaaaacagaa gttgcttcaa
      661 gaatcgaata tgatgatatg cgtcatatga caaatatgat atttaatcaa atatattaat
      721 ctgttacatc agcatatctt ggtattcgtc aatgtgaata tgacaaatga tcttgcagaa
      781 taaactcaga accgctttat aggatcgtta cgctacgaaa acgacacgtg attgtgggag
      841 tgaacagtga atgaagtcag ggaaggatag gggctgttac gaatgggaga cagaagctac
      901 tgattctcga caccgtcgcg tcccagaaat cgtttcatga gcagcgagaa atgttgccgt
      961 ggaattccgc tctcttcagc agccttggtg atgacaccac cgtgttgcgc gagtttaaca
     1021 cgcagatagg acttcgtaaa ttcttcaagt gctcgctctt tcgcttccat ataggggagg
     1081 aaggtcagag tagggtcgac cgaggcgaga ctctcggcct cattctttgg gagcatgcct
     1141 cggacatcgc gcccccggat gatgtcgcca gtcgcgagca tgaccatccg ttccacgaca
     1201 tttcggagtt cccggccatt tcccggccaa gagtaagaca caagggctct caccgcctct
     1261 tcatcaaagt ggcactgcgc cttgcccatg gactgaaaca gccggctgag caaatgacgc
     1321 gcgataacgg gaatatcctc gcgacgctca cggaggggtg ggaccggaat cgagatgact
     1381 gcgagtcgat agtacaaatc cttccggaag gtcccctcac gcactcgtgc ttcgagattc
     1441 cggttggtcg cagcgataaa gcgcatatcc acccggcgaa ctctggttcc acctacggga
     1501 cgcacctcgc tttcctccag tacgcgcagg agtttcgctt ggagaggaag gggaagctcc
     1561 cctatttcat caaagaacac gctgccgccg tttgctaact ctatgagccc tggcttatcg
     1621 gcaatggcac cggtaaacgc gccccgtaca tgcccgaaca gctcggactc catcatcccc
     1681 tcgggaacgg tcgtacaatc gatcacttga aacggccgat cgcgctctgg actctgctga
     1741 tgaatcgccc gtgcaatcat tccctttccc gttccggttt caccggtaat gaggaccgtc
     1801 gaacggactt gagccgcctc ttgaatactc cgcatgatat gctggaagga gatgccactc
     1861 cccacaaaac tattggcttc gaattcctgc cggtcacgat gctcatagac cgcatgccgg
     1921 cgcaggattt cagatatgcg aatcgcccga tggagtcgca ttcccaaatc ctctgcattg
     1981 acgggtttca gcacatagtc aaaggcgcct cgccgcatgg catccaccgc cagagggacc
     2041 gcattcgtcc atgtgtacac cagcactggc aaatttgaat cgatcccttg aatgcggctg
     2101 acgagatcca acccatccat cccaggcaac accatatcag tcagcacaag atcgacggac
     2161 cgttccccga gtaattgcaa ggccccttcc gccgaggcac gaacctccat ctcgaaatct
     2221 cccccaagcg gtttcaaatc cgccgcttca agagcgtgga gcatcaactc aacatccgtt
     2281 gcgttgtcct cgacgatcag aatcgtaaac ggtgctctag atttggttct ggtcgatcgc
     2341 atgtcggtca ctccgccatg tgttatcatc tccccatcct cacagaaccc ctcgcgggac
     2401 gacatcaacc acgccgggaa gcctcgactc ctgcatccgg acattcgagg tcccctcatg
     2461 ctcctcaaaa ctcgggaccg tgaatagtac cgtgcagccc ggcccctcgg ctccgtcgat
     2521 ccacacctta ccgccataca gttccacgat tcgctggatg atagtcaggc cgatcccgct
     2581 gccggctgct tcagacatca acaagcggac aaacggctga aacactcgat tgcgttgggt
     2641 caaagggatc ccgatccccc tatcctccac agaaaatacc accagagggc cctctacacg
     2701 cccgctgatc ctcaccacgg gtgactctcc agcccttgtg aacttcagtg cgttcgagac
     2761 gaggttgtcg aagatttgtc ggagataggc accatgacag gcgacgagcg gcaaccccgg
     2821 ttcaatgtgc accacagcgc ggagccgttc gatctcaccg gctttggctt tcaatacctg
     2881 atcaataatc gcagtgggat cgacagccgt gatggcaccc tgatcagccc cgacgcgagc
     2941 caccgtcaag atcccttcga ctcgttgaac aagatccctg ccattctctt caataagaga
     3001 gagccaccgg ttcgtccggg catcaaattg acgaccgttc tcctggacaa gtaaccgtgc
     3061 gagttctccc atgcgagccc ccggcccctt gagatcgtga gctaacgttt ccgcaagatg
     3121 ctcgagctgt tggaggcgtg cagctaaccc ccgttgccgc tgcgctgcgg cttcatacaa
     3181 acgcgcattt gagatagcca gggcagcctc ctgggccaat ccttcggcca gacgcagatt
     3241 ccactcctgt gctccccggg gtcgttcggc cagaagcgac a
//
LOCUS       DATIPF010000067         4498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1602898, whole genome shotgun sequence.
ACCESSION   DATIPF010000067 DATIPF010000000
VERSION     DATIPF010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602898"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VLL06_01720"
     CDS             <1..709
                     /locus_tag="VLL06_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29715.1"
                     /translation="LVIGKIGRFLDPIITVRAPKGDQVEKYWTPNGNHRLSAMKTLGA
                     KTIVAIVMPETAAAYQILAMNTEKAHNLREKSLEVIRMYKELARLDDTMEEAYSLEFE
                     EPAFITLGLCYETRPRFSGGAYHPLLKRVDDFLKKPMRTAMELRQERAKTVLALDDQI
                     VQQVEALKAKGLTSPYLKSFVVARVNPIRFRPKDASPLSFDEALARMASATAKFNPGK
                     IKMEDVARSGGAVDGAE"
     gene            822..1172
                     /locus_tag="VLL06_01725"
     CDS             822..1172
                     /locus_tag="VLL06_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HSS29716.1"
                     /translation="MSLADNKCIPCRGGVPPVPSDRAQALLKELGRGWSLNQVGHLER
                     LYTFKDFALALAYVNKVGAVAEAEGHHPDFYLAWGKCKIEIWTHKINGLTESDFYMAA
                     KADREFEPFRAVAG"
     gene            1215..3074
                     /locus_tag="VLL06_01730"
     CDS             1215..3074
                     /locus_tag="VLL06_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HSS29717.1"
                     /translation="MSDKAKVALVNMPFSYSKFPSIQLGTLSALLKSKGTPVDCHHLN
                     VRFASKIGVPLYEIICEKRALFGEWIFSYLLFRDNPKRTEYPRLFKPVFEQITKESGH
                     PVSFFEDMATRVAPQFLTWALTAIDWGQYRIVGFTSTFDQNVASLTMAKLIKDLYPDV
                     TIVFGGANYDGEMGLEYFRAFPFIDHVVIGEGEETFPALVRTILDGKGERVPNGVTYR
                     QGDQIKFVPNDTLFSDFTNTGPPDYDDYYHLLAELGETARGLDRILLYEGSRGCWWGE
                     KHHCTFCGLNAQNMKFRAKSSEQVAREMAFLSSRYDTTRFRLVDNIIDMHYIEALFGR
                     FAADHCDLDVFIETKSNLQKSQIRTLAAGGVKCMQPGLESLSINQLRAMDKGVTPIQN
                     IISLKWSYYYHVAVSWNILLGFPGETDEDYRRQIDLLPSLFHLQPPESTGKFWLQRFS
                     PYYSRPHEYGVRITGPGTAYEFIYDPQQVDLKKIAYDFEYELENWNVDPHVYQELIDL
                     VGEWQRLVGSSDRPFLYYSKAMDYVTVYDGRNPKTPIRRRFDWPAAGIIEVCNEAAKS
                     ADQIRTLLASRLDKKGNSDVEIDHALDALTAARILYEERGKYFTLAVPENSYL"
     gene            complement(3522..3806)
                     /locus_tag="VLL06_01735"
     CDS             complement(3522..3806)
                     /locus_tag="VLL06_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29718.1"
                     /translation="MARAMSPSGDSNGNPPQAAGAELHTKDVELLRVLVSRKGTHVDA
                     QWAIHPQLKQDLLPQEWQEVTDLMAKVTGIVGTRFSQVLTQADPEQPSNA"
     gene            complement(3912..>4498)
                     /locus_tag="VLL06_01740"
     CDS             complement(3912..>4498)
                     /locus_tag="VLL06_01740"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HSS29719.1"
                     /translation="LTEQPPSRYKWVIDPLDGTTNFAHGFPAYCVSIGVECDGRGIIG
                     VVYDPSRDELFTAKIGQGAYLNGVPISVSTTDHLDRALLVTGFAYNIRETPNNNLNHF
                     VRFALKVQGLRRTGTAALDLCYVAAGRFDGFWEVTLNPWDMAAGVVILREAGGTVTDF
                     NGAPHSIYGQELVASNGPIHQAMLDLLQDDVTRS"
BASE COUNT         1039 a         1199 c         1246 g         1014 t
ORIGIN      
        1 actcgtgatc ggaaagatcg ggcggtttct agacccaatc attacggtcc gagcgccaaa
       61 gggtgaccag gtcgagaagt actggactcc caatggcaac catcggctct cggcgatgaa
      121 gacgttgggg gcgaagacca tcgtcgcgat tgtgatgccg gaaactgctg cggcctatca
      181 gattctcgca atgaataccg agaaagcaca taatctgcgc gaaaaatcgc tcgaagtcat
      241 tcgcatgtac aaggaattgg ctcggctcga tgatacgatg gaagaggcct attctttgga
      301 atttgaggag ccagcattta tcactctcgg cctgtgctat gaaacacgac cgagattcag
      361 cggcggggcc taccatccgc tgttgaagcg ggtggacgac tttctgaaga aaccaatgcg
      421 cacagcaatg gaactgcggc aggaacgggc aaagactgtg ctggcgctgg acgatcagat
      481 cgtccagcaa gttgaggcac tgaaagccaa aggcctgacg agtccatatc tcaagagctt
      541 tgtcgttgct cgcgtcaacc cgattcggtt ccggcctaag gatgcctctc ctctctcatt
      601 tgatgaagcg ctcgcccgga tggcgagtgc aacagccaaa ttcaacccgg gaaagattaa
      661 gatggaagac gtggcccgct cgggcggcgc agttgatggg gctgagtaag cactcattat
      721 ctaattcccc accttgccct tcttcgcgta gcccctttag aatacttgat ctagatagag
      781 tcgacacaca ggctgtgatg ttcataccag gaggtggtgc catgagcctt gctgataaca
      841 aatgtatccc ttgccgcggc ggcgtacctc cagtcccgtc ggatcgtgcg caggctctcc
      901 tgaaagagct ggggcgcggg tggtcactga accaggtcgg ccatcttgag cgtttgtata
      961 cgttcaagga tttcgccttg gccttggcct acgtgaacaa ggtgggagcc gtggcagagg
     1021 cagaaggcca ccatcccgat ttctatctcg cctggggcaa gtgcaagatc gagatttgga
     1081 cgcataagat caatggcctg acggagagtg atttttacat ggcagccaaa gccgatcgcg
     1141 agttcgaacc gtttcgcgcg gttgccggct agccgcgagt cggacggttg cgtcatgaat
     1201 ccacgaggaa acagatgagt gacaaggcca aggtggcgct cgtcaatatg ccgttcagct
     1261 actcgaagtt tccctcgatc cagctgggca cactctccgc gctgctcaag tcgaagggga
     1321 cgccggtcga ctgccatcat ctgaacgtac gatttgcttc caagatcggc gtacccctct
     1381 atgaaataat ttgcgagaaa cgggcgttgt tcggcgaatg gattttttca tacctcttgt
     1441 tccgcgacaa tccgaagcgt actgaatatc cacgtctgtt taagccggtg ttcgagcaaa
     1501 tcaccaagga gagcggccat ccagtctcgt tcttcgaaga catggcgaca agggtggcgc
     1561 cacaattctt gacctgggct ctcaccgcaa tcgactgggg gcagtacagg attgtcggct
     1621 tcacgtccac gttcgatcaa aacgtcgcga gcctgacgat ggcaaagctc atcaaggatc
     1681 tctatcccga cgtaacgatc gtcttcggtg gagcaaatta cgacggtgag atggggttgg
     1741 aatatttccg agcatttccc ttcatcgatc acgtcgtcat cggtgaggga gaagagactt
     1801 tcccggcatt agtccgcaca atcctagacg ggaagggtga acgagttcct aatggagtca
     1861 cgtaccggca aggtgaccag attaagttcg taccgaacga cactctcttt tcggatttca
     1921 cgaacacagg tccgccggat tatgacgact actatcacct gcttgctgaa cttggcgaaa
     1981 cagcccgtgg gctcgatcgt atcctgctat acgaggggtc acgcggctgc tggtggggtg
     2041 agaagcatca ctgcacgttc tgcgggttga acgcgcagaa catgaaattt cgcgccaaat
     2101 cgtcggagca ggtggcacgc gagatggcct tcctgtcgag ccgatacgac accacccgat
     2161 tccgactggt ggacaacatc atcgacatgc actacatcga agccttgttt ggaaggtttg
     2221 cagccgatca ttgcgatctc gacgtgttca tcgaaaccaa aagcaaccta cagaagagcc
     2281 agattcggac acttgctgcc gggggcgtga aatgcatgca gccggggctg gagagtctga
     2341 gcatcaatca gctgcgcgcg atggataagg gtgtcacgcc gatacagaat attatctccc
     2401 tcaagtggag ctactactat catgtcgcgg tatcctggaa tatcttgctc gggtttcctg
     2461 gagaaacgga tgaggactac cggcgccaga tcgacttact tccgtccttg tttcatctgc
     2521 agccacctga gtccaccggg aaattctggc tgcagcgctt cagtccctac tacagcaggc
     2581 cgcatgagta tggtgtccgt atcaccggcc cggggacagc ctatgagttc atctacgatc
     2641 cgcagcaggt ggacttgaag aagatcgcgt acgatttcga gtatgagctg gaaaattgga
     2701 acgtcgaccc acacgtctac caagaattga tcgacctggt gggagaatgg caacgattgg
     2761 tcggttcgag cgatcggccg tttctctact actcgaaagc gatggactat gtcaccgtgt
     2821 acgacggtcg caacccaaag acaccgatac ggcggcgctt cgattggccc gcggcaggga
     2881 tcattgaggt ctgtaacgaa gcggccaaga gcgctgacca aatccgcacc ctgctggcaa
     2941 gccgactcga caagaaagga aacagcgacg tagagattga tcacgcactc gatgcattga
     3001 cggctgcacg aattctctac gaagagcgcg gcaagtactt cacccttgcc gttcccgaaa
     3061 actcctacct ctaactgaac cacataccgg tctcgttaca tcgacagttt gtcggcgaca
     3121 ttggggatct gaacgccgtg cgcgagcgag gcaccgcctc ggatggcgca aacaacatgg
     3181 acagcttccg tcatttcatc gatgtttcga ccctatttca agtgaagcct gcgtgtacgc
     3241 atcaatgcag tacgggcatt ggactgcgtg agcaacggca agggcaatga gggccttctc
     3301 gcgttcggtg agccccttca gcaaagcgct gtaggtaggc catgaatttc ttccggagat
     3361 ccttgttgcc tttgcccatc tcggcgaact tgcccgaatc atgggggtga taataggtct
     3421 ccatcggtca ctccaaattg cgaatgttac agcgtgaacg gtgaatgaag gagggcatgc
     3481 tcattcacca ttcacaccca tcattcaaca ttattgtgtg atcatgcgtt gctgggctgt
     3541 tcgggatcag cttgcgtgag cacctgcgaa aaccgggtgc caacgatgcc ggttacttta
     3601 gccatgaggt cggtcacctc ttgccattct tgtggcagta gatcttgttt cagttgagga
     3661 tgaatcgccc attgggcatc gacgtgtgtt ccttttcggc tcacaagcac ccgaagcagc
     3721 tccacatctt tagtgtgtaa ttcggcccct gcagcttggg gtggatttcc atttgaatcg
     3781 cccgatgggg acattgcacg tgccatacag tcaatctcct tctctctggt cgaaggaatc
     3841 ataccgcatc gtcattctgt ctgtatatct gacgggtggc ctctgtggcc gagggcagcg
     3901 ggactgatac cttacgaacg agtaacgtcg tcttggagca ggtctagcat ggcctgatgg
     3961 ataggtccat tacttgccac caattcttgt ccataaattg agtgaggagc accgttgaag
     4021 tccgtcactg tgccaccagc ttctcgtagg atgaccactc cagccgccat atcccacggg
     4081 ttgagtgtca cttcccaaaa tccatcgaaa cggcctgctg cgacgtagca gagatccagg
     4141 gctgccgttc cggttcggcg caatccctgc accttcaatg cgaatcgtac gaaatggttc
     4201 aaattattat tcggtgtctc gcggatgtta tacgcaaatc cggtcacgag gagcgcacga
     4261 tcaagatggt ccgttgtgga cacggaaatg ggaactccat tgaggtaagc cccctggcca
     4321 atcttcgcgg tgaacagttc gtctcgactc gggtcgtaga cgaccccgat aatcccacgt
     4381 ccgtcgcact ccaccccgat ggatacacag taggcgggga acccgtgggc aaagttggtc
     4441 gtgccatcca gtggatcgat gacccatttg tagcgggatg gaggctgttc ggttagcc
//
LOCUS       DATIPF010000068         5784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1690485, whole genome shotgun sequence.
ACCESSION   DATIPF010000068 DATIPF010000000
VERSION     DATIPF010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690485"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VLL06_01745"
     CDS             complement(<1..217)
                     /locus_tag="VLL06_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29720.1"
                     /translation="MMCCRNDGTSRIIPLTTMSESTPPTIDHTLALREEFRHHLETFY
                     AQLKLAPPYESVEKAIRSLTTSIHALPP"
     gene            complement(295..1335)
                     /locus_tag="VLL06_01750"
     CDS             complement(295..1335)
                     /locus_tag="VLL06_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29721.1"
                     /translation="MQIRTASRLAQFASLMVVLFCAGCQNESEAIVSIALHPTNANIL
                     YVATNDAVYKSRDEGRLWERFPSFSARRVTTLAIDPQFPATIYAGTMADAVYKSPDGG
                     QHWMPHNVGLKEHVSFINQFVFDPTDHEQIYAATTVGAFYTKDGGREWEERMAGMKEV
                     HIVVSIAIHPRDARVLYAGTTGGVYRSDSAAMGWKKINNGLIPEQELMGAMALGVNVI
                     VFDVVNPDIVYAGTTKGLFRTATRGEQWERIGQSLPDLFVSSILLDPTQPKTLYIGGP
                     GGVWKSLDSGQSWTATNNGLASLNIRTLAMSPRDSHILYAGTNGSGLYRSTDAGATWT
                     PLPLKAAPTPAQ"
     gene            complement(1638..2765)
                     /locus_tag="VLL06_01755"
     CDS             complement(1638..2765)
                     /locus_tag="VLL06_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HSS29722.1"
                     /translation="MYDLWRQLRHIVLLSSSLLGVVPGLAADTPEEFRLATEGYRYAF
                     PRDHGAHEEYRTEWWYYTGQLTAKNGRPFGYELTFFRRGMPREETKTLPSQWAVTHLY
                     LAHFAVSDLRQGRFHYAEKMSRAGLGKAGAASDRLNVWIDRWSAESPLSAPGTQILHA
                     ADGDLAIQLTVSPEKPLVIHGTGGISRKGSVAASHYYSFTRLATTGTLSVGGERFDVT
                     GNSWMDHEFGSAELGKDLVGWDWFCLQLDDHRELMLYHLRRTDGSADQASSGTLVDRD
                     GRGHHLTVSEFTLEPISYWTSPTSHARYPQRWRLTIPSQQLSFELVPLMAEQELSTTR
                     STQVTYWEGAIEATGSIQGRAARAQGYMELTGYAERIPNKL"
     gene            2886..3623
                     /locus_tag="VLL06_01760"
     CDS             2886..3623
                     /locus_tag="VLL06_01760"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HSS29723.1"
                     /translation="MVRAVDNFVGHQLSWAMQTDHGREPHGRDQRDTVTPFPIPARIP
                     VFPLPNVVLFPKTYLPLHIFEPRYRTMVSDAAMNGQCIGMALLKDGWEADYYGHPPVF
                     PLGCVGRLASVQPLADGRSNILLQGLERFEIEHEWYDKAYREATISVAVRGTEASLDP
                     MVRRRLFGFLESYLQSRADVPTWQELFREEVSDEIVVNTLSTYLECTPLEKQFLLEAE
                     SLHQQARRLSDLIEFMMHDQSGAKGWG"
     gene            3623..4597
                     /locus_tag="VLL06_01765"
     CDS             3623..4597
                     /locus_tag="VLL06_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSS29724.1"
                     /translation="MDRAVAIQVSHLTKSYDGHTAVSDLSFQVYAGEIFGLLGPNGAG
                     KSTTLRTLITLLTPTSGTASVLGHDTVREADAVRKLIGYVPQERAIDRFLTGREHLEL
                     LGALYHLPKAEATKRIAELLKLVELEEPADRAAKTYSGGMKRKLDIACGLLPDPKILF
                     LDEPTLGLDVQSRLRIWDYVRMLKARGMTIVMTTNYLDEADQLCDRLAIIDGGKIKTL
                     GSPTELKVGLGGDIVSLTLKDQGKIATLVSALTRQPAMRAVKATPTGLDIRVESPEKA
                     LPAILEAANRLDCQLEFIEYHRPRLDDVFIAHTGRSIKDDVPVEKEGT"
     gene            4688..5467
                     /locus_tag="VLL06_01770"
     CDS             4688..5467
                     /locus_tag="VLL06_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSS29725.1"
                     /translation="MTEYWQEISALTMRWVRRLSREKFSMLFTLVQPMLFWLIFFGNL
                     FQRAADMQVTQAPDYISFLTAGVVVMTVLNNGLAGGVDLLFDKENGFLERLMTTPIHR
                     SSVILSRFIFVMTITSLQVLVIIGVAFLFGVQPATGLLGIAAILMIGMLFGVGLTAIS
                     MAMAFSVKSHGDFFSVLGFLSLPMIFLSSALVPLAGMPAWMGFLARFNPMTWAIDAVR
                     PLILSGWTEALPHVGMVVVVMVIFDALCLYGSAKVFRRAMG"
     gene            5494..>5784
                     /locus_tag="VLL06_01775"
     CDS             5494..>5784
                     /locus_tag="VLL06_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29726.1"
                     /translation="MLINESQIRALIRLLADEDEKIVRTISGKLIDIGPTAVPLLQEA
                     EIEQPEMAHRLVSVLEEIRGGDLEDELMQRTALPDGSMDLEHGAFLIARYAYP"
BASE COUNT         1169 a         1565 c         1760 g         1290 t
ORIGIN      
        1 acggcggcag agcgtgaatc gaggtcgtga gggaacgaat ggctttctca acgctctcat
       61 agggtggcgc cagcttgagc tgggcataga acgtttcgag gtgatggcga aattcctctc
      121 gaagcgccag ggtatgatcg attgtaggag gagtcgattc agacatggtg gtgagcggta
      181 tgatacggga agtcccgtcg ttgcgacaac acatcatcca tgtggatgtc caagcaggct
      241 cttcaatcac cggatacaga tagccgcgaa aattcttgca gcgcgaggga ccggttactg
      301 agccggtgtt ggtgcggcct tcagtggcaa gggcgtccag gttgcaccgg cgtcggtcga
      361 gcggtagagg ccgctcccgt tcgtgccggc gtagaggatg tgcgaatcac gcgggctcat
      421 ggcgagggtt cgaatgttca gcgaggccaa tccgttgttg gttgcggtcc aggattgtcc
      481 gctgtcgaga cttttccaga cccctcctgg tcctccgata tagagagtct tcggctgggt
      541 cggatcgagg aggatactgc tgacgaacag atcggggagc gattgcccga ttcgctccca
      601 ttgttctcca cgtgtcgcag tgcggaagag acccttcgtg gttccggcat agacgatgtc
      661 cgggttgacg acatcgaaga caatgacatt gactcccaag gccatcgctc ccatcagttc
      721 ctgttcggga atgagcccgt tgttgatttt cttccacccc atcgcggcgc tgtcggaccg
      781 atagacccct cctgtcgttc ccgcatagag aaccctggca tcgcgaggat gaatggcgat
      841 ggagacgacg atgtggactt ctttcatccc ggccatgcgt tcttcccatt cacgtccacc
      901 gtctttggta taaaaggccc cgacggttgt ggcggcgtag atctgctcat ggtctgttgg
      961 gtcaaagaca aattgattga taaaggatac gtgttctttc agtccgacgt tgtggggcat
     1021 ccaatgttgc ccgccatccg gactcttgta gacggcgtcg gccatcgtac cggcatagat
     1081 cgtcgcgggg aactgagggt caatggcgag agtcgtcacg cgtctggcgc tgaaactcgg
     1141 aaatcgctcc cacagccggc cctcgtcgcg tgacttatag acggcatcgt tcgtggcgac
     1201 atagagaatg ttcgcgtttg tcggatgcag ggcgatagag acgatcgcct cgctctcatt
     1261 ttggcaaccg gcacagaaca gcaccaccat cagagaggcg aactgagcga gccgagaagc
     1321 ggttcgaatc tgcatgggtg tggtggctgg aggcctaaca gctcatggac aacagatgaa
     1381 tatcatacag gatgctcaaa aataccgccc agcaaggcca cagcgagcga agaggcgagg
     1441 aggtacgtac cgcacatcgt gtgcggccgt tcgcccttac atataggtct tggccaacgg
     1501 aaaaacccct ctcgtgtttc ccacctccga gatactcaat gttgagcctc tgagcgatgc
     1561 gagaacgccg ctggcggaca ttttcagcat cctgctaatc ataggattca gcgatgagtc
     1621 aagatgccca gattccgtta caacttattg ggaatacgtt cggcgtagcc ggtcagttcc
     1681 atgtagccct gtgcacgggc ggcccgccct tgaatggagc cagtcgcctc gatcgcgcct
     1741 tcccaatagg tgacttgcgt actgcgcgtg gtggagagtt cttgctcggc catgagcgga
     1801 acaagttcga acgagagttg ttgggaggga atggtcagac gccaacgctg aggataccgt
     1861 gcgtggctgg tcgggctggt ccaataactg attggctcca gggtgaactc actgaccgta
     1921 agatggtgcc cacgtccgtc tcggtcaacg agggtgccgc ttgaggcctg atcggccgat
     1981 ccatcagtcc ggcggaggtg atagagcatc agttccctgt ggtcgtcgag ttgcaggcag
     2041 aaccagtccc atccgaccag gtccttgccc agttcggcag acccaaactc atggtccatc
     2101 caactgttcc cggtgacatc gaatcgctca ccgccgactg agagtgtccc ggttgtggcc
     2161 agtctggtga aagaatagta atgggaggct gcgaccgagc ctttgcggct gataccgcct
     2221 gtgccatgta tgacgagtgg tttctccggc gagaccgtga gttgaatggc caggtcaccg
     2281 tccgctgcat ggagaatctg cgtgccggga gcggacaatg gcgattctgc actccagcga
     2341 tcgatccaga cattgagccg atcactcgcc gcaccggctt tgcccaaccc ggcccggctc
     2401 attttctccg catagtgaaa acgaccctga cgaagatcgc tcacggcgaa atgggccagg
     2461 tacaggtgag tgacggccca ctgtgacggc agggttttcg tctcctcgcg cggcatgcca
     2521 cggcggaaga aggtcagctc atagccgaag ggtcggccgt ttttcgccgt aagctggccg
     2581 gtgtagtacc accactctgt gcgatactcc tcatgcgctc cgtgatcgcg cgggaacgca
     2641 tatcgatagc cttctgtcgc gagtcggaat tcctctggcg tatcggctgc gagtcctggt
     2701 acgacgccca gcagactgct cgacaacaag acaatatgtc gaagctgacg ccacaaatca
     2761 tacatgtcag atgggcgctc cggacgggat agaaatcgtc gcgtgcgctt ataccatgct
     2821 tcgggataca gcacaaatcc cctgtgttga tgcgggtttt attgaattct gtcggtgaaa
     2881 gatgcgtggt tcgcgccgtt gacaactttg tcggccatca gctatcgtgg gccatgcaga
     2941 ccgatcacgg gcgagaacct cacggacgcg accagcgcga cacggtgacg ccgtttccta
     3001 ttcccgcgcg gattccggta tttccgcttc caaacgttgt gctgttccct aagacctatc
     3061 tcccgctcca tatctttgag ccgcggtacc gcaccatggt ctcggatgcg gccatgaacg
     3121 ggcagtgtat cggtatggcc ctgctgaagg atgggtggga agcggactat tatggccatc
     3181 cgcccgtgtt ccccctgggc tgtgtcggtc gattggccag cgtgcagccg ctcgccgatg
     3241 gtcgatctaa cattctgctg caagggcttg aacggtttga aattgaacac gaatggtacg
     3301 acaaggccta tcgtgaagcc acgatttccg tcgcggttcg aggcacagag gcgtcgctgg
     3361 atcccatggt tcgccggcgc ctgttcggct tcctggagtc gtatctccag tcccgcgctg
     3421 acgtgcccac atggcaagag ctctttcggg aagaagtgag tgatgagatc gtcgtcaata
     3481 ccctgtccac gtacttggag tgtacgccgt tggaaaagca gtttctgtta gaggccgaga
     3541 gtctccatca acaagctcgc cgtctcagcg atctcatcga attcatgatg cacgatcaat
     3601 ctggtgcgaa gggctggggt tgatggatcg cgccgtcgcc atccaggtct ctcacctgac
     3661 gaaatcctat gatggccata ccgccgtttc cgatctgtcc tttcaggtct atgcaggtga
     3721 gatcttcggt ctgctgggac cgaacggggc agggaaaagc accacactcc ggacgctcat
     3781 cacgttgctg acaccgacgt cgggcaccgc gagcgtctta ggccacgata cggtgcgcga
     3841 ggcggatgcg gttcgcaaac tcatcggtta cgtccctcaa gaacgggcca ttgatcgctt
     3901 tctcactggc cgcgaacacc ttgaactcct cggcgccctg taccatttgc ccaaagccga
     3961 agccacgaaa cggatcgccg aattgctgaa gctcgtggaa ctggaagagc ctgccgatcg
     4021 cgcggcaaaa acatactcag ggggaatgaa gcgaaagctc gacattgcat gcggactgtt
     4081 gccagacccc aagatcttgt tcctcgacga gccgacgttg ggcttggatg tgcagagccg
     4141 gctccggatc tgggactatg tgcggatgtt gaaagcgcgg ggcatgacga tcgtgatgac
     4201 gacgaattat ttggatgagg cggaccagct ctgcgatcgg ttggccatca tcgacggggg
     4261 aaagatcaaa acgctcggat cgcctactga gttgaaggtt ggactggggg gcgacattgt
     4321 gtctttgaca ttgaaggatc agggtaagat cgccacgttg gtgtcagcgt tgacgagaca
     4381 accagcgatg cgagcggtca aggccacgcc gaccggcctg gatatccgag tcgaatcgcc
     4441 cgagaaggcc ctccctgcga tcctggaggc cgccaatcga ttggattgcc aattggaatt
     4501 tatcgaatat caccggccga gactggatga cgtgttcatt gcccatacgg gccgatcgat
     4561 caaagacgat gttcctgtag agaaggaggg tacgtgatac ggtatacgtg atgcgagaag
     4621 tcgaggcggc gggatcctcg cagcttcgat taggacgctt cacgtgtgac gtgtcaggct
     4681 tcaagtcatg acagaatact ggcaagagat cagtgcctta acgatgcggt gggtccggcg
     4741 gctgagccga gagaaattca gtatgctgtt cacgctggta cagccgatgc tgttctggct
     4801 gatcttcttc ggcaacctgt tccagcgggc ggcggatatg caagtgacgc aggccccgga
     4861 ctacatcagc ttcctgaccg ccggtgtggt ggtgatgacg gtgctcaaca atggacttgc
     4921 cgggggcgtc gatctgctat ttgacaagga aaatgggttt ctggagcggt tgatgaccac
     4981 gccgatccat cggagttccg tgatcctgag ccgctttatc ttcgtcatga cgattacctc
     5041 gctccaagtg ctggtgatca tcggagttgc ctttctgttc ggtgtccagc cggcgaccgg
     5101 acttcttggt attgccgcta tcttgatgat cgggatgctg tttggcgtcg gcctgaccgc
     5161 gatctctatg gcgatggcct tttcggtgaa gagccacggt gatttctttt cggtcctggg
     5221 attcctttcg ctcccgatga tattcctgag ttcggccttg gtgcccttag cggggatgcc
     5281 tgcgtggatg gggttcctgg cgcgcttcaa tccgatgacc tgggcgatcg acgcggtgcg
     5341 gccgttgatc ctttccggct ggactgaagc actgccccat gtcgggatgg tggtcgttgt
     5401 catggtaatt tttgacgcgc tctgtctcta cgggagcgcc aaagtattcc gccgcgcgat
     5461 gggctgatga gcgggcgttt cgggagtccc cccatgctga tcaatgagag tcaaattcgt
     5521 gccctgattc gattgctggc cgatgaggac gaaaagatcg tccgcaccat cagcggtaag
     5581 ctcatcgaca tcggtcccac ggcagttccg ctgcttcagg aagcggagat cgaacagccg
     5641 gagatggctc accgtcttgt ctcggtgctc gaggaaattc gcggtggaga tctcgaggat
     5701 gaactgatgc agcggacggc gctgccggat ggctcgatgg atcttgaaca cggggccttt
     5761 ctgatcgctc ggtacgccta tcct
//
LOCUS       DATIPF010000069         5180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1514760, whole genome shotgun sequence.
ACCESSION   DATIPF010000069 DATIPF010000000
VERSION     DATIPF010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514760"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /gene="pstA"
                     /locus_tag="VLL06_01780"
     CDS             <1..1100
                     /gene="pstA"
                     /locus_tag="VLL06_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HSS29727.1"
                     /translation="GMGHALSPSTIDSPAPVMKETGQIKDLRAQIAAAEARYQEKTLE
                     LDALRTQFNEYSVVMVDANGREKQIPVGQIIRALRPNEMSLLYKSWIYLTNVWSVLSS
                     EPREANTEGGIFPAIFGTVMMVIVMSLAVVPFGVLAALYLREYAKQGALVRLVRIAVN
                     NLAGVPSIVFGVFGLGFFIYAIGGTLDTLFFPERLPTPTFGTGGILWASLTLALLTVP
                     VVIVATEEGLSAVPREFREGSLALGATKLETMLKVVIPSALPGILTGLILAMARAAGE
                     VAPLMLTGVVKLAPSLPVDSSWPFLHFDRKFMHLGFHIYDVGFQSPNVEAAKPMVYIT
                     TLVLITVVVLLNFTAIVLRNHLRKKYAGSVL"
     gene            1110..1979
                     /gene="pstB"
                     /locus_tag="VLL06_01785"
     CDS             1110..1979
                     /gene="pstB"
                     /locus_tag="VLL06_01785"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HSS29728.1"
                     /translation="MYSSPPMTQQPPQSQPVTSPRLNVPENHLQSSDNRSHAHAVKLA
                     LHQVNFFYGDHQALFKIDIEIMKNQVTSFIGPSGCGKSTLLRCLNRLNDLIEGARVEG
                     AMLLDGIDINGHDVDITDLRKRVGMVFQKSNPFPKSIYENVAYGPRLHGIRNRSQLDQ
                     IIEEGLKGAGLWDEVKDRLQASAFGLSGGQQQRLCIARALAVKPDVLLMDEPCSALDP
                     VATAKIEELLFSLKQDLTVIIVTHNMQQAARVSDWTAFMYLGELVEFGLTKQLFTTPS
                     KKQTEDYITGRFG"
     gene            1985..2674
                     /gene="phoU"
                     /locus_tag="VLL06_01790"
     CDS             1985..2674
                     /gene="phoU"
                     /locus_tag="VLL06_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HSS29729.1"
                     /translation="MTQRHFDDELADLKTTLLRMAGLAEDQIDKALTALVTRDSSLAR
                     HVIERDHQVNTLDVEIDEECIRLLALHQPAARDLRLVTTAMKISTELERISDLAENVS
                     ERTIELNEEPQLKPYIDIPLMGKMARTMVKESIDAFVKDDSALARKVLTDDDFVDDLM
                     EQLFRELLSFMLEDTRTISRAIRLSFIAKYLERMADHATNIAELVVYLVEGKIIRHTI
                     PPGASDRDLAL"
     gene            2829..3065
                     /locus_tag="VLL06_01795"
     CDS             2829..3065
                     /locus_tag="VLL06_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29730.1"
                     /translation="MLCEELGGKKRVLEPMSPQDDFVASGELIDAVEDLIPGILRHQA
                     NERIQTDDGLLIEMVENGCGESIGRRRLQVNIRI"
     gene            complement(3108..3623)
                     /locus_tag="VLL06_01800"
     CDS             complement(3108..3623)
                     /locus_tag="VLL06_01800"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HSS29731.1"
                     /translation="MTDITMEPTLPSGWLKRSFITLAAFDMTRVWEVGTRFILSLLTL
                     TILIGLAGGVIKTFIDLRLLLSADVEVALRQILVDTLTLLAVVEVLKTTLAYCSDGRV
                     RVTFIIDTVLVVMLTEVISRWFTSGDWQQFTILGGILLTLGAMRIVAVRYSPAPTTVK
                     QQAHQLGLSFP"
     gene            4193..4438
                     /locus_tag="VLL06_01805"
     CDS             4193..4438
                     /locus_tag="VLL06_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29732.1"
                     /translation="MTSVDSLMLEAKQAILDEHQRRFQTLHQEGRWEEAIHQIHVTLR
                     CAADLLDESLRVLDEAIETHKGIASDSSTPPPPLTTT"
     gene            4620..>5180
                     /locus_tag="VLL06_01810"
     CDS             4620..>5180
                     /locus_tag="VLL06_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSS29733.1"
                     /translation="MPTAPKKILIVEDEPDIAQLVKLYLEKEGFHTTVANSGVDALKL
                     MKAERPDLLILDLMLPEIDGIEVCKKIRTVPDTALLPIIMLTAKAEESDTIIGLELGA
                     DDYVTKPFSPKALVARVKALFRRLERTDDHKQSSFSFGPLFMDLSKHEVTVKGKEVSL
                     TAKEFGLLEHLLRHPGRVLTRDVLLNT"
BASE COUNT         1258 a         1390 c         1351 g         1181 t
ORIGIN      
        1 ttgggatggg acatgctctt tctcccagca ccatagactc tccggccccc gttatgaaag
       61 agacagggca gatcaaggac ctcagggcac agatcgctgc cgctgaagct cgttatcaag
      121 aaaaaacctt ggagttggac gcgctgcgca cacagttcaa tgaatactcc gtcgtgatgg
      181 tagatgcgaa cggccgagaa aagcagatcc cagtcgggca aatcattcga gccctgcggc
      241 cgaatgagat gagtctcttg tacaagagtt ggatctatct cacaaacgtc tggtctgtct
      301 tgagcagcga gccaagagaa gccaatacgg aaggcggcat tttccctgcc atttttggaa
      361 ccgtcatgat ggtcattgtc atgagcctcg ccgttgtccc tttcggcgtg cttgccgcgc
      421 tctatctgcg agaatatgcc aagcaaggcg ccttggttcg tctcgtgcga attgccgtga
      481 acaaccttgc aggtgtaccc tcgattgtct ttggtgtgtt cggattgggc tttttcatat
      541 acgccattgg agggacgctc gatacgctct tctttcctga acgacttcca accccgacat
      601 tcggcaccgg tggcatccta tgggcatccc tgacattggc gttactcacg gtgccggtgg
      661 tcatcgtcgc cacagaagaa ggattgtctg ccgtgccacg ggagtttcgt gagggatcat
      721 tggctcttgg ggcaacgaag ttggaaacga tgctcaaagt cgtgataccc tcagcgctgc
      781 cgggcattct gacgggattg atcttagcga tggcccgggc ggccggcgaa gtggcgccgc
      841 tcatgttgac tggggtcgtg aagcttgcgc cttcgctccc ggtagacagt tcctggcctt
      901 ttctccactt cgatcgaaag tttatgcact tgggatttca catttacgac gtgggctttc
      961 agtcccccaa tgtggaagcc gcaaaaccca tggtttacat tacaaccctg gtattgatca
     1021 ccgtcgtggt gctcttaaac tttaccgcga ttgtcctccg taaccacctg cgcaagaagt
     1081 atgcaggatc ggtgttatag aggatcgtga tgtactcatc tccacccatg acacaacagc
     1141 cgcctcaatc acaaccggtc acctcaccaa ggctcaacgt gccggagaat catctccaat
     1201 cgtctgacaa ccgttcccat gctcacgccg tgaaacttgc gctccatcag gtcaatttct
     1261 tttatggcga ccaccaagcg ctcttcaaaa ttgacattga gatcatgaag aatcaggtca
     1321 cgtcattcat tgggccatcc ggctgcggaa aatccactct gctgcgatgc ctcaaccggc
     1381 tgaacgactt aattgaaggc gctcgagtag aaggcgccat gctgcttgac ggcatcgata
     1441 tcaatggtca cgacgtggat atcaccgacc tcaggaaacg tgtcggcatg gtctttcaga
     1501 aatcgaaccc ctttccgaag tcgatctatg aaaacgtggc ctatgggccc cgtcttcacg
     1561 gaatccgaaa tcgcagccaa ttagatcaaa tcatagaaga agggctcaaa ggtgcagggc
     1621 tatgggatga ggtgaaagat cgcctccaag ccagcgcctt tggtctgtct ggaggtcaac
     1681 aacaacgtct gtgcatcgcc cgcgccctcg cggtcaaacc ggatgtgttg ctgatggatg
     1741 aaccctgctc ggcgctggat cctgtggcca ctgccaagat tgaggaattg ctcttttcct
     1801 tgaagcaaga tctgacagtt attatagtca cacacaacat gcagcaggcc gcccgggtgt
     1861 ccgattggac ggcatttatg tacctcggcg aactggtaga gttcggcctc acgaagcagc
     1921 tctttaccac gccttccaag aaacagacgg aagactacat caccggacga tttggatagt
     1981 cggcatgaca cagcgccact ttgacgatga actagccgat ttaaagacca cgctcctgcg
     2041 catggccggc ctggcggaag atcagatcga caaggccctc actgcgttgg tcacacgcga
     2101 ctcgtcctta gcgcgccacg ttatcgagcg ggaccatcag gtcaacacgt tggatgtgga
     2161 gatcgacgaa gaatgtatcc ggttgcttgc cctccatcag cctgccgcac gagatctgcg
     2221 gcttgtgacg acggcaatga aaatctccac cgagctcgag cgaatcagcg acctggcgga
     2281 gaacgtctcc gaaaggacga tcgagctgaa cgaagaaccg caacttaagc cctatatcga
     2341 tatcccactg atgggtaaaa tggcgcggac gatggtgaag gaaagcatcg atgcctttgt
     2401 gaaggatgat tccgccctcg ctcggaaggt gctcacggac gacgacttcg tcgatgatct
     2461 gatggagcaa ttgttccgag aactgctgtc attcatgctc gaggacacgc gcacgatttc
     2521 acgcgccatt cgtttgagct tcattgccaa atatcttgaa cggatggccg accatgctac
     2581 caacatcgcc gagttggtgg tgtatctggt agagggcaag atcatccgtc atacgatccc
     2641 gccaggagcc tctgaccgcg atctcgcgct gtaatacgcc tctgtccctc ctcaagtttt
     2701 ccttgaatgt tgagtgatga gaagagccaa acctcaccac atgacatatg tggttcaatg
     2761 gcaatactgc tccccggcca actatggggc attcagcgtg agtgaataga tgtggcctga
     2821 ggaagggcgt gctatgcgag gaattgggtg gaaaaaagcg tgtactcgaa ccgatgtcgc
     2881 cgcaggacga ctttgttgca tcgggagagt tgatcgacgc ggtggaagat ttgattccag
     2941 gtatattgag gcatcaggca aacgagcgta tccaaactga tgacggtctg ctcattgaga
     3001 tggtcgagaa tggctgcggt gagagcatcg gacggagaag gctccaggtg aacattcgca
     3061 tctgattcaa gaagagaact tataggcatc ggtacctcca agtaagatta tggaaatgaa
     3121 agccccagtt gatgggcctg ttgcttgacc gtcgtcggtg ccgggctgta tcggacggcc
     3181 acaatacgca tggcacccag cgtgagcaag atgcctccca gtatcgtgaa ttgctgccag
     3241 tccccgctcg tgaaccatcg tgagatgacc tccgtcagca tcacgacgag gaccgtgtcg
     3301 ataataaacg tgacacggac ccgaccgtca gaacagtatg cgagagtagt cttcaacact
     3361 tcgacgacgg ccagcagagt cagcgtgtcg accaatattt ggcgaagggc cacttccacg
     3421 tcggcggaca gcaacagccg cagatcgata aatgtcttga tgactccacc tgccaaacca
     3481 atgagaatgg tcagggtcag caggctcaag atgaatcgcg tgcccacttc ccagactcgt
     3541 gtcatgtcaa aggccgccag cgtgatgaac gatcgcttga gccagccgct cggcaatgtc
     3601 ggttccattg tgatgtctgt catagtgtta atcatagccg gacacatcga tccaggtatc
     3661 tcgctcatgt gaatcgatga agagcatgac gcggattaca agagtacacc gcacgcctat
     3721 tgccaagact ccccttctgt catgaccttc tcgacgaact tccacgcgcc ataacctgaa
     3781 accgggtgga gcgaatcgag catccttttc tcatggttca atcctacccc ggacatattc
     3841 cggtcgagtc aattacccgt aaactctgtg taaactgtct atcgagcgga gtgactgagt
     3901 tcttccctgg tcgtgtgaaa ctggcggcca cattggtgat gccggagtag agcgaggtac
     3961 gcagatgaga gtaagttgag gattgtcgac caaggaggga atgcgaacga caagaattgt
     4021 gattactgtt tgatcaatcg tcagataatt tataactgaa tattgacaac ccaataatgc
     4081 aatatcttat gcttcatagt taatctatta cattacccac ccattcgtat cattgttgta
     4141 aagacaccgg gtctgttacc gtgcgcctcg atcacacaaa tgaaaggcgc ccgtgacttc
     4201 agtcgacagt ctcatgctcg aagcgaaaca agcgatcttg gatgaacatc agcgccgttt
     4261 tcaaaccctc caccaggaag gccgctggga agaggcgatc caccaaattc atgtgaccct
     4321 acgctgtgcg gcagacctcc tcgatgagtc actccgagtc cttgacgaag ccattgaaac
     4381 tcacaaaggt attgcgtcgg atagctccac tcccccgcct cctcttacaa cgacctgaag
     4441 atcagcggtt cttctacgct tctctcccgt gacagagcag ggttcgtcct gagtcacatt
     4501 gtcgaaagtt ctccttaaag cccatcatcc tgcctgaaac attcccgtgc ataggtttcg
     4561 ttaatacaac cgtaacattg aaggattatc ttagcgcatt gtcatgagag ggagaacaga
     4621 tgcccacagc tcccaagaag attttgatcg tcgaagacga accggatatt gcccagttgg
     4681 tgaagctcta tttggagaag gaagggtttc acacgactgt cgcaaactcg ggagtcgatg
     4741 cactgaagct catgaaggcc gagcgtccgg accttctgat tctggacctc atgcttccgg
     4801 aaattgacgg cattgaagtc tgtaagaaaa tccgtaccgt gccggacacc gcgctccttc
     4861 ccatcatcat gctgacagcc aaagccgagg agtccgatac catcattggg ctcgaactcg
     4921 gggccgacga ctatgtgacg aaaccgttca gtcccaaagc gttggtcgcg agagtgaaag
     4981 ccctctttcg ccgcctcgaa cggacagatg atcacaagca gtcatccttt agttttggtc
     5041 ccctcttcat ggatctctcc aagcatgagg tcaccgtgaa gggtaaagag gtgtcactga
     5101 cggccaagga attcgggttg cttgaacacc tgctgcgtca ccctgggcgc gtgctgacac
     5161 gagatgtcct gctcaacacc
//
LOCUS       DATIPF010000070         3513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1603455, whole genome shotgun sequence.
ACCESSION   DATIPF010000070 DATIPF010000000
VERSION     DATIPF010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603455"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VLL06_01815"
     CDS             complement(<1..621)
                     /locus_tag="VLL06_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HSS29734.1"
                     /translation="MSPDAKGLACSFGKTGFLIPPSASSLPAAEPDWYQRACHALQEI
                     KRAVQTRQPWTLDELTLIASGVVASLRVNDKLVQAALRHNDGDYLINNAVNVAVVSVK
                     IAEALGYESAKLEQVALAGLLHDLGMFILPDALVYKVGTLTDEDLQHVREHPQHGARV
                     FKEVGDAYPWVEKVILQEHERRDGSGYPHQLVGDHIDEMAFIIGLAD"
     gene            complement(713..898)
                     /locus_tag="VLL06_01820"
     CDS             complement(713..898)
                     /locus_tag="VLL06_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29735.1"
                     /translation="MPNLVRIVIQLPRDLKTQLDNLKQQGYTTSGFIRAMLEREFAKM
                     EEKPVVPISSGKRVNSR"
     gene            complement(1141..>3513)
                     /locus_tag="VLL06_01825"
     CDS             complement(1141..>3513)
                     /locus_tag="VLL06_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HSS29736.1"
                     /translation="LLVGVIYWSVAVSLTAHDPMIEQSFDAVIDVTGRIVAPVQPAPD
                     RLVMVIRSDNQVPASRPSRHIRLTWRTPDRMFFQGDRVAFRAMLRSPSGSLNPGGFDY
                     AVYLERQGIDAIATVTGSEAVQFLESGRTNAWWTIWNQLDRWRSSIRLAALQTLPQPA
                     LGLYVGIIIGDRGYLDPDLRDQFMATGTVHLLSISGSHLGLVALLIFAVVRWVMIVLP
                     ADWLLAMTRKVTPTRIAAVCTFVPVAGYAFLAGAELATMRSLLMVTVGLSAIWLGQER
                     RLFHALSAAALAILLHDPQALFDISFQLSFLSVFALAGWLSWPATHERQTLLDEPSLL
                     KSCARWGRDAIVMSGVITVVTLPLVAYYFNQLPWLGLLANVVAVPVMGILLVPIGLGA
                     AVWQIIVGGSLLPLASLNQWFLEHFVAAVRLVSMLPGVEWHVAAPSALTMVMFYSCLA
                     LLWWRVGGTAFRSAVGAGVLLLLLWWAWSPRIFLDGDHFRVTFLDVGQGDSAVVELPD
                     GQVVLIDGGATYERFDMGRGVVAPFLWNRGIRTIDQVIGTHPQLDHVGGLAWVIRHFI
                     VKHYWGSGETREEVFYRRLQHSLATQGLQEGVAREGQEIVSSGPCRMVVLNPPAGEHL
                     DVSLHDSTMNGHELNNHSVVTRLTCGTHTMLFAADVERDGLSRMTHSTSQGSIEVLKV
                     PHHGAVSSLNRDWLASVHPQYAVFSAGRHNPYKHPATVVLDAYVSEGSTLLRTDRDGG
                     VWFTGGVADTSFHVYRARDLVWQPTSRASCLWACEQANWDRVVKLWRDRY"
BASE COUNT          884 a         1060 c          874 g          695 t
ORIGIN      
        1 gtctgccagc ccaatgatga atgccatctc atcgatgtga tcgccaacta gttggtgagg
       61 atagccgctt ccatcccgtc gctcatgttc ctggaggatc accttctcca cccatggata
      121 ggcgtctccc acttctttga atacacgggc tccatgctgg ggatgctccc tcacatgctg
      181 aaggtcttcg tccgttaacg ttccgacctt gtagaccagc gcatccggta agatgaacat
      241 accgagatcg tgcagcaaac cggccaacgc cacctgttcg agcttcgcag actcgtagcc
      301 tagcgcttcg gcaatcttca cactcacgac cgcaacattc acggcattat tgataagata
      361 gtccccgtca ttgtgtcgga gagccgcctg aaccagtttg tcattgactc gaagagaggc
      421 gacgacacca gatgcgatca aggttaattc gtcgagcgtc caaggctgcc tcgtctgaac
      481 tgcacgcttg atttcctgaa gcgcatggca ggcacgttga taccaatcgg gctcagcagc
      541 aggcagtgag ctggcggaag gtgggatgag aaaaccagtc ttcccaaagg aacaggcgag
      601 accttttgca tcaggactca tggcgtcctg actcgactgt cctcgaataa gatcacttaa
      661 cctcgtcaca gctccctcag ccctcttcca ccctctggcg catcagaaac agctaccggc
      721 tattgactcg tttcccactg gaaatgggta cgacaggttt ctcctccatc ttggcaaact
      781 cccgctccaa catcgctctg atgaagccac tcgtcgtgta tccctgctgc ttcaggttat
      841 cgagctgggt tttgagatcg cggggtaact gaatcacaat tcgtacgaga tttggcatgc
      901 cttagctccc ttaaaatatg gtacgcacat gctctccggc acgagtgaat gcacatgcta
      961 tgccttgcta gcaagtgatg aaagatgaac gaagttgagg tcttatgcta tgaagctttc
     1021 actggggata aacgcagaga cgaaaatcgc ctagccaaga cgtgaaatgt gcactctctc
     1081 acgcgtagga aactggtggt cctgtgccaa agatccatat ggtatggccc aaaacgatgg
     1141 tcaataacgg tcccgccata gtttcacaac tcgatcccaa tttgcctgtt cacaggccca
     1201 caggcacgag gctcggcttg ttggctgcca caccaagtct ctcgctcgat atacatggaa
     1261 cgaagtgtcg gcgaccccgc cagtaaacca gaccccgccg tcccggtcgg ttcgtaacag
     1321 cgtactccct tccgacacat atgcatctag aacaaccgtg gctggatgct tgtaaggatt
     1381 atgacgacca gccgaaaata cggcgtactg aggatgcacg gatgccaacc aatcacgatt
     1441 caatgaactc accgctccgt gatgaggaac tttcagtacc tcgatcgaac cctgagacgt
     1501 cgaatgcgtc atccgcgaca agccgtcccg ttcaacatcc gcagcaaaga gcatcgtatg
     1561 agtcccacat gtcagacgcg tcaccactga gtgattattc aactcatgcc cgttcatcgt
     1621 gctgtcgtgg agcgacacat caagatgctc gccagctggt gggttgagaa ccaccatccg
     1681 acagggaccg gatgagacaa tttcctgccc ttctcgagcc accccttctt gtaacccttg
     1741 tgtcgccaat gagtgctgca gtcgtcgata gaacacttct tcgcgtgttt ctccagatcc
     1801 ccaataatgt ttcacgataa agtggcggat cacccaggca aggcctccga catgatcaag
     1861 ctgagggtgt gtaccaatca cctggtcgat ggtgcggatt ccccgattcc agagaaacgg
     1921 agcgaccacc ccacgcccca tgtcaaatcg ctcatacgtc gcccctccgt caatcaacac
     1981 cacctgtcca tcaggcagct caacaaccgc gctgtcgcct tgcccgacat ccaggaacgt
     2041 gactcggaag tgatctccat cgagaaagat tcgtggggac caggcccacc acagcaatag
     2101 taagaggact cccgcgccta ctgccgaccg gaatgccgta ccgcccactc gccaccacag
     2161 cagtgcaaga caactataaa acatcaccat ggtgagagcc gaaggggccg ccacatgcca
     2221 ttccactccc ggtagcatcg ataccaaacg aaccgcagcc acaaagtgtt cgagaaacca
     2281 ctgattcagc gaggccaagg gcaacaggct tcccccgact atgatctgcc agaccgcggc
     2341 accaagtcct atcggaacca gcagaatccc catcacggga accgctacga cgttggcaag
     2401 gagaccgagc cagggcaatt gattgaaata gtacgcaaca agaggaagcg tcaccacggt
     2461 gatgacccca ctcatcacta tcgcgtctcg cccccatcgg gcgcaggatt tcagtaacga
     2521 cggctcatcc agcagtgtct gtcgttcgtg tgtggccggc caggagagcc accccgctaa
     2581 ggcgaagacg gacaaaaagg acagctgaaa ggagatatca aacagcgcct gaggatcatg
     2641 cagcaaaatt gccagggcag cagcagagag ggcatgaaag agccggcgct cctggcccag
     2701 ccagatcgca ctcagaccca cggttaccat cagcaacgac cgcatggtag ccagttctgc
     2761 tccggccaga aaggcatagc ctgcgacagg aacgaacgtg cagacagcgg caatacgagt
     2821 cggcgtgact ttccgggtca tcgcaagaag ccagtccgcc ggcaatacaa tcatgaccca
     2881 tctgacgacc gcgaagatga gcagtgcgac gagacccaag tggctccctg aaatagaaag
     2941 gagatgcacc gtccccgtcg ccataaattg atcacgcaaa tctggatcca aatatcctcg
     3001 atctccgatg atgatgccca catagagtcc caacgcaggc tggggaagag tttgcagcgc
     3061 agcaagacgg atactgctcc tccaccgatc taattgattc cagatcgtcc accaggcatt
     3121 cgtgcgtcca gactcaagaa actgcactgc ctcggatccg gttaccgtag cgatcgcatc
     3181 gattccttgc cgttcaaggt agaccgcata gtcgaatcca ccaggattca atgacccgct
     3241 cggactgcgc aacattgccc gaaaggcgac ccgatcgcct tggaagaaca ttcgatccgg
     3301 tgtgcgccag gtcaaccgaa tatgtctgga tggtctcgac gcgggaacct gattgtccga
     3361 cctgatgacc atgaccagtc ggtctggcgc tggttgcacc ggtgccacga tacggccggt
     3421 cacgtcgatc accgcatcga acgactgctc gatcatcggg tcatgagcag tcaaactgac
     3481 ggctacactc caatagatga ctcccaccaa gag
//
LOCUS       DATIPF010000071         6452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_717230, whole genome shotgun sequence.
ACCESSION   DATIPF010000071 DATIPF010000000
VERSION     DATIPF010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717230"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="VLL06_01830"
     CDS             <1..1371
                     /locus_tag="VLL06_01830"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HSS29737.1"
                     /translation="IGYGSPHKQDTYEAHGSPLGHEEVILTKQALGWPIDPPFYVPDQ
                     ALVHFRRAQEQGKRAEAEWEERFSAYTQAFPDLAKEYQQRMMGELPRDWDRPIPLFQA
                     DPKGLATRVVSGKVLNAIVHTVPSLLGGSADLDPSTHTALAGMGDFQAPRDEVDNLQG
                     STGGGWSYAGRNIHFGVREHAMGSIANGLAVHGGIIPFTATFLIFSDYMRPAIRLAAL
                     MELRVIFVFTHDSIALGEDGPTHQPIEQLASLRAIPNLLVIRPCDANETAVAWRIGIE
                     SRDRPVALILTRQDVPTLDRTNLAAADGVRRGGYVLTDIPGGNVPDIILMATGSEVSL
                     IVAVGRQLAQHQIKVQIVSLPSWELFETQPRSYRDSVLPPSVGTRLAVEAGATQGWRK
                     YVGDDGDVIGVDHFGASAPGKVLMRQYGFTVDDVYARAMKLINLKGQAWIDTRLSQQQ
                     EARGKK"
     gene            1397..2569
                     /gene="tal"
                     /locus_tag="VLL06_01835"
     CDS             1397..2569
                     /gene="tal"
                     /locus_tag="VLL06_01835"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HSS29738.1"
                     /translation="MIGNPLIQLKAFDQSIWIDFIQRGMISSGQLQRLHEDDGVSGVT
                     SNPSIFEKAIADSQDYDSAIRALALEGKSIDQIYDAITVEDIQRTADVFRPIYDRLNG
                     ADGFVSLEVSPHVAHDTAGTIEDARRLWAAVARPNVMIKVPATPEGLPAIQELIGEGI
                     NINTTLLFGLPRYRQVVEAYMTGLENLVAQGKSLRSVTSVASFFLSRIDVLLDPMLDR
                     LIVRDPSKADVAHDLRGQVAILSAQAAYQIYKELFESERFRKLKRQGARPQRLLWAST
                     STKNPAYSDLKYVEALIGRETIVTMPLETLMAYRNHGKPASRLTIEGAMKSRETLQRL
                     GEMEIDLDAATTQLEYEGVHKFVKAFDLLMQTLEKKRTAAVRSLADVATGNPGMKV"
     gene            2642..3779
                     /gene="zwf"
                     /locus_tag="VLL06_01840"
                     /pseudo
     CDS             2642..3779
                     /gene="zwf"
                     /locus_tag="VLL06_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
     gene            3776..4063
                     /locus_tag="VLL06_01845"
     CDS             3776..4063
                     /locus_tag="VLL06_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29739.1"
                     /translation="MRLRPVGMRFRYRDTFARPSPEAYETLLWDVMNNDPTLFMREDQ
                     IEASWRFVMPVLEVWANTRPRKFPNYSAGTWGPKAAEQLIERDGHTWLKSA"
     gene            4429..6363
                     /locus_tag="VLL06_01850"
     CDS             4429..6363
                     /locus_tag="VLL06_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSS29740.1"
                     /translation="MNQIVLIALRRPYTFLVMSILIVLLGSKTVLHMPTDIFPNITIP
                     VTSVVWIYSGLLPKQVEGRITYLFERFLTSTVEGIKYIHSHSYYGSSITNIYLQDGVE
                     VGRAEADIVGIAQNVVKALPPDISPPMVMRLAPSSIPVAMLEISSDDMTPAELYNLAY
                     MRIRPLLVTVPGIVLPHPYGGQDMQVMVNLDQQNLLARHLTPADVHDILMRQYRVLPS
                     GDIKIHSTDWIVQTNASPLKIEDFDNIPIKREGNAFVYLRDIATVRLMGRVQQNMVLV
                     KGKQVVIIVAMKSTEASTLDVVDGIKKMIPHAEQVSPKGVKIRLLDDASTFVKDSISD
                     VVREMAMAGALVGLIVLMLLGSWRATALVWTSIPLSILTAIIGLHALNETINVMTLGG
                     LALAVGILVDNATVVIENIDRHLAMDKPLEQAIIDATKQIVLPTFVATLCIAIVWYPL
                     FELGGVAGYLFKPMAEAVIIAMLASFVLSFTLVPTMAKYMMKSTAMPSSYDQRGQPDA
                     ETPRNVLVRYQKEFERHFNRFREGYGMLLEQVIAHRRPFVTISLAIVVGSLSLFFFLG
                     RDYFPEIRSGVIQMHMRAPLGTRIEVSGRLANLVSNSIEELLPGQVENIVSNCAPSPR
                     CRAVRSARRGECRDSARAPR"
BASE COUNT         1467 a         1725 c         1791 g         1469 t
ORIGIN      
        1 ataggatacg gctcgcccca caaacaagac acgtatgaag cccacggctc gccgcttggc
       61 cacgaagaag tgatcttgac gaaacaggca ctcggctggc cgattgaccc accgttctat
      121 gttccagatc aggcactcgt ccatttccgt cgcgcccagg aacaaggcaa acgggcagag
      181 gcagaatggg aggaaagatt ttcagcctat acacaggcgt tccccgatct tgccaaggaa
      241 tatcaacaga gaatgatggg cgaattgccg cgcgattggg atcgccccat cccgttgttt
      301 caggcagatc cgaagggtct cgcgacgcga gttgtatcgg gtaaagtgct caacgcgatc
      361 gtccacacag ttccgtcgtt gctcgggggt tctgcagatc tggatccatc cacccacacc
      421 gcgcttgccg gcatgggcga cttccaagct ccgcgcgatg aagttgacaa tttgcaaggg
      481 tcaacgggtg gcggctggag ttatgcgggg cgcaacattc actttggcgt ccgtgaacat
      541 gcaatgggct ccattgcgaa tggactagcc gtgcatggcg gtatcattcc attcacggca
      601 acattcctca ttttctcgga ctatatgcgt ccggcgattc gattggcggc tctgatggaa
      661 ctgcgggtca tcttcgtgtt cacccacgat agcatcgctc tgggagaaga tggtccaacg
      721 catcagccga ttgagcaact ggcgtcctta cgagcgattc ctaacttact ggtcattcgt
      781 ccctgcgatg cgaatgagac ggctgtggca tggcgcattg gtatcgagag tcgggatcga
      841 ccggtcgcgc taattttgac acggcaagat gtgcccaccc ttgaccgcac gaaccttgcc
      901 gcggcagatg gtgtgcgacg tggtggctat gtgttgaccg acattcctgg tggaaacgtt
      961 cctgacatta ttttgatggc cactggatcg gaggtcagct tgatcgtggc cgttggcaga
     1021 caattggccc aacatcaaat caaggttcag atcgtctccc ttccaagctg ggaattattc
     1081 gagacccagc ctcgaagtta tcgcgactcg gttctccctc cctcagtggg cacgcggctt
     1141 gcggttgagg ccggtgccac gcaaggttgg cggaagtatg tgggcgatga tggtgacgtg
     1201 attggagtag accattttgg tgcgtctgcc ccgggaaaag tgctcatgcg tcagtatgga
     1261 tttacggtgg acgatgtata tgcacgtgcc atgaaattga tcaaccttaa aggtcaagcg
     1321 tggatcgaca ctcgtctatc ccagcaacag gaagcgaggg ggaagaagta atcaaccttg
     1381 gagatacgga gatactgtga tcggtaaccc tctgatccaa ctgaaggcgt tcgatcaaag
     1441 catctggata gattttattc aacgaggaat gatttcgtcc ggtcaactac agcgactcca
     1501 tgaagatgat ggagtaagcg gcgtgacctc caatccatcc atttttgaaa aggccattgc
     1561 agacagccag gattatgaca gtgccatccg tgcactggcg ttggaaggaa aaagtatcga
     1621 ccagatctac gacgccatta cggtggagga cattcaacgc acggccgatg tatttcgtcc
     1681 gatctatgat cggctgaacg gtgcggatgg gtttgtgagt ctcgaagttt ctccgcacgt
     1741 tgcccacgac accgccggga ctatcgaaga cgcacgacga ctatgggcgg ccgtggcgcg
     1801 cccaaatgtc atgatcaaag tacctgcgac tcctgaagga ttgccagcga ttcaagagct
     1861 catcggtgag ggaatcaaca tcaacactac gctgctgttc ggattgccta gatatcggca
     1921 ggtcgtcgag gcgtatatga ccggtcttga aaatctagtt gcgcaaggca agtcgctgag
     1981 atccgtgaca tctgtcgcca gctttttctt gagccgtatc gatgtactgc tcgatccgat
     2041 gctggacagg ctaatcgtca gggacccatc aaaggccgat gtcgcccacg atctccgggg
     2101 acaggtggca atcttgagtg cccaagccgc gtatcagatt tacaaagaac tgttcgagag
     2161 tgagcgattc cgcaagctga agcgtcaagg tgcgagacct cagcgtttac tgtgggcgag
     2221 caccagcacc aagaatcccg catacagcga tctgaaatat gtggaggctc tcatcggtcg
     2281 tgaaaccatt gtgacgatgc cgttggagac actcatggcc tatcgaaacc acggcaaacc
     2341 ggcatctcgt ctaacaatcg agggagcaat gaaatctcgc gagacacttc aacgattggg
     2401 cgaaatggag attgacctcg acgcggccac cacgcagctc gaatacgagg gtgtccacaa
     2461 gttcgtcaag gcattcgatc tcttgatgca gaccctggag aagaaacgca ccgccgcggt
     2521 gcgatcgctg gccgatgtgg caacaggcaa tcctggtatg aaagtgtaat ggaatatgga
     2581 gggttctccg agcattgttg tgatttttgg tggggcgggt gatttgacct ggcgtaaagt
     2641 catgcctgct ttgtttgatc tgcatcggaa gggtcagatg tccaaacagt tcgcgataat
     2701 tgccatagat cgtgccactc tccgtgatgc gcaggcgcac aaacgctacc ttgatggtgt
     2761 gaaccgtttt tcgccgaatg ggaaggcgaa aggtacagat tggccgcaat tctcccggca
     2821 tgtcacctat tcagcagggg gatttgacag accaagccac ctacgcacat ttgggagaac
     2881 gctgcgcaca attagatcaa cagtggaagg tcaaagccac tcatgtattt tatatggcta
     2941 ctccaccgag catgatggga gtgatccccg acatgctcgc tgctgtgggt ctgaatagag
     3001 atcatgcccg tgcacggatt gttgtggaga aaccgatcgg tcacgacttg gattcagcgc
     3061 gtgaactaaa tcgcaagctc aagaaaagtt ttcatgaggg acaaattttt cgcatcgatc
     3121 attacctggg aaaagagacg gtccaaaaca ttctcacctt ccgcttcgcc aatccactgt
     3181 ttgagccgat ttggaatcac caatatgtgg actacgtgac catcacggcc gccgaagcac
     3241 tggggatagg acatcgtggt cggtattacg atcaagccgg aggattacgc gatatggttc
     3301 aaagtcacct catgcagctc ctctgtctgg ttgccatgga accgatggtc tcgatcaatg
     3361 ccgatgagat ccgtaacaag aaagtcgatg tactccatgc actacgtcca atccggctcc
     3421 aggacgtcca tcaatatgcg atacgcggcc agtatggcag gggaaccgtt aacgggaaac
     3481 aggtccaggg gtatcggagt gaagagggca tcgctccgca ctcacatacc gaaacatatg
     3541 tggcgttgaa ggcatttatt gacaattggc gatggcaggg cattcctttc tatctgcgca
     3601 cgggcaaata tatgccacgc cggttttcgg aagtggccat ccactttcga ccggtgccac
     3661 atcagtcttt ttcgccggac atctcctgtg gctggtatcc atctcgactt gtgctctcaa
     3721 tccagcctga agaagaaatt atgctgagct ttcaggccaa gatccccgga cttcaatgag
     3781 gctgcggccg gtcgggatgc ggtttcgtta tcgcgatacc tttgcaagac cctcgccgga
     3841 agcctatgag acgttgctct gggacgtgat gaataatgat ccgacattgt tcatgcgtga
     3901 agatcagatc gaagcctcat ggcgattcgt gatgcccgtg cttgaagtgt gggctaatac
     3961 tcgtcctcgg aagtttccta attattctgc aggtacatgg gggcccaaag ccgctgagca
     4021 gttgatcgaa cgtgatggcc atacatggtt gaagagcgcg tgagaaagct ggtcatcgaa
     4081 gggctggctg cgctttcttc acccgacatc atgccgtagc gtctatttac ccaaacccga
     4141 catggtgcct gtaccaacag ccttactttc cagccaaaga tcttttgtcg gtcattcata
     4201 gattcccatg tttcttttct gagcccggca cctacgcagt tcgagcatct tagccaatga
     4261 tttgcctcac tggacgaaga agccgcacgg ctggaacaag ggtgttttcc tagggagcac
     4321 gctagttgta tgaatccaag aataaaccat actggataac ttacggttgg agtgaggatt
     4381 cagactggta gatctgatga cgctcgacga tcaattaagg tgtcgcggat gaaccagatc
     4441 gtcctcattg ctctgcgacg gccgtatacc ttcctcgtca tgtccatcct gatcgtactc
     4501 ttgggcagca aaacagtact ccacatgcca accgacatct ttccaaatat cacgattccc
     4561 gtcacctccg tcgtctggat ctattccgga ctgctgccga agcaggtgga gggacgcatc
     4621 acgtatttgt tcgaacgttt cctgacctcg acagtggaag gcatcaagta cattcatagt
     4681 cattcgtact acggcagcag cattaccaat atttatctgc aagacggggt ggaggtgggc
     4741 agggccgaag cggatatcgt gggcattgca caaaacgtcg tcaaggcgtt gccgcccgac
     4801 atttccccgc ccatggtcat gcgtctggcg ccatcttcta taccggtggc catgctcgaa
     4861 atcagctccg acgacatgac gcccgcggaa ctctacaacc tcgcctacat gcgtatccgt
     4921 cccctgctcg tgacagtacc agggatcgtt ctgccgcatc cgtacggagg tcaggacatg
     4981 caggtcatgg tcaatctcga tcagcagaat ttgctcgccc gtcatctcac tccggcggac
     5041 gttcacgata tcctcatgcg gcaataccgc gtgctgccgt ctggggacat caagatccat
     5101 tcgaccgatt ggattgtgca gacgaatgcc tcaccgctga agatcgagga ttttgacaat
     5161 atcccgatca agcgggaagg caacgcattc gtctatctgc gcgacatcgc cacggtgcgc
     5221 ctcatgggcc gggtgcaaca gaacatggtg ctggtgaaag gcaaacaggt cgtcatcatc
     5281 gtcgcgatga agagcacgga ggcttcgacc ctggacgtgg tggatgggat caaaaagatg
     5341 attccgcacg ctgagcaagt ttccccgaag ggcgtgaaga ttagactgct ggacgatgcc
     5401 tcgaccttcg taaaggattc catttcggat gttgtgcgtg aaatggcgat ggctggcgcg
     5461 ctcgttggcc tcatcgtgct gatgttgctt ggctcatggc gggcgacggc cctggtctgg
     5521 acgtcgatcc cgctttccat cctgaccgca atcatcggat tgcatgcgct caacgagacg
     5581 atcaatgtca tgaccttggg cggactggcg ctggccgtag gcattctagt cgacaatgcg
     5641 actgtggtca tcgagaatat tgatcgccat ctcgccatgg acaagccgct cgagcaggcc
     5701 atcatcgatg ccaccaagca aatcgtcctc ccgacgttcg tcgccaccct ctgcatcgcg
     5761 atcgtctggt acccgctttt cgagctcggc ggcgtcgccg gttatctgtt caagcccatg
     5821 gcggaggcgg tcatcattgc gatgctcgcc tcgttcgtcc tgtcgttcac gctggtgccg
     5881 accatggcaa aatatatgat gaagagtacc gccatgccgt cgtcttatga ccagcgcgga
     5941 cagcccgatg cggagacacc gcggaatgtt ttggtccgct atcagaagga gttcgagcgc
     6001 cacttcaacc ggttccgcga gggctacggt atgctgctcg aacaggtgat tgcccatcgc
     6061 cgcccctttg tcacaatcag tctggcaatc gtggtgggat ccctgtccct cttcttcttc
     6121 ctcgggcgcg attatttccc cgagatcagg tcgggggtca ttcagatgca tatgcgggcg
     6181 ccgctcggga cacgcatcga ggtatcagga cgcctagcca atctggtctc taacagcatt
     6241 gaggagttgc tgccaggcca agtcgaaaac atcgtcagca attgcgcgcc gtcgccgcgg
     6301 tgtcgagccg tccgatcagc acgtcgcggc gaatgccgag atagcgcgcg tgcgccgcgt
     6361 tgacgtgatc gccgtgcgct tcgagcgtca cgcgggcagc ctcgagactc ttctccggcc
     6421 agccgagatc gcgcgaggcc agtgcaatct cc
//
LOCUS       DATIPF010000072         6435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_543277, whole genome shotgun sequence.
ACCESSION   DATIPF010000072 DATIPF010000000
VERSION     DATIPF010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543277"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VLL06_01855"
     CDS             complement(<1..306)
                     /locus_tag="VLL06_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSS29741.1"
                     /translation="MKVLVTGGAGFIGSHVVDRLIQEGHEVVVVDNLSTGKRRNLNRA
                     ARFCKLDIQSWRLERVFRNERPNIIMHLAAQMDVRKSVEDPIFDAQVNVLGMLNVLQQ
                     "
     gene            complement(361..1119)
                     /locus_tag="VLL06_01860"
     CDS             complement(361..1119)
                     /locus_tag="VLL06_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HSS29742.1"
                     /translation="MASTPRAHKERLDRVLMVQGLVSSREAAVRTVLAGGVSVDGTMV
                     DKPAKLVSLDARIEVVQPALFVSRSGDKLAAALDAFHFDPRGLIGLDVGCSTGGFTDC
                     LLQRGATRIYAVDVGYGQFEWRLRQDSRVVLLERTNIRYVDRAAVPDPIDLAVVDVSF
                     ISLTLVLPAVVRLLKRSAQVVALVKPQFEVGKGQVGRGGIVRDDAQREAVTEKVIACA
                     ADLGLQLKGVLDSPVIGRKGNREILVGFVREATT"
     gene            complement(1180..1428)
                     /gene="xseB"
                     /locus_tag="VLL06_01865"
     CDS             complement(1180..1428)
                     /gene="xseB"
                     /locus_tag="VLL06_01865"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HSS29743.1"
                     /translation="MAAVKFEQAMARLEVIVGELEKNDLPLDESLKIFEEGIRLSKNC
                     LKILEEAEHKVEVLVQDKNGKKRIHAYTSDGEADELSS"
     gene            complement(1485..2813)
                     /gene="xseA"
                     /locus_tag="VLL06_01870"
     CDS             complement(1485..2813)
                     /gene="xseA"
                     /locus_tag="VLL06_01870"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HSS29744.1"
                     /translation="MFTVSELTGLLRTSIEEQFSDILLEGELSNLRAPGSGHVYCTLK
                     DKTSQIRAVLFRSSAVRLRFSLQEGLHVLVRGRLTVYEPRGEYQIVLDRVEPKGIGAL
                     QLAFEQLKERLAVEGLFDQERKQAIPAFPRTVGVVTSLTGAAIRDILAVLRRRWPTLH
                     ILIAPVQVQGESAGRQIAEALTALNELDSVDVIIVGRGGGSLEDLWSFNEEIVVRAIA
                     ASHIPVVSAVGHEVDVTLADFVADFRAPTPSAAAETVVPVLAEIVERVRELQVRVGQV
                     MLQHCATERQRLEAGIRGVTDIRFRLQEASQRTDDMVDRLREMVQQLLTSGRERVHEV
                     QRDLSGLNPILAIKQGLVTIPQFSKRLEGYMGVLLAQHRHRIHATLAQLNTLSPLAVL
                     GRGYSILHTVPSGQILRRAQDVEVGQELEARLASGRLSCLVTRIFDDSSV"
     gene            complement(2880..3659)
                     /locus_tag="VLL06_01875"
     CDS             complement(2880..3659)
                     /locus_tag="VLL06_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HSS29745.1"
                     /translation="MKVLFIGDIFGEPGRRALARAVPRLVGQRQIDIVIGNGENAAGG
                     FGITPELAEELFDLGLAVITTGNHAWDKREILDYFPREPRLLRPANYPDGVPGHGSVV
                     VESAGGEKLGVLQLMGRAYMPTLDCPFQVAKKELAALKKRTAAVIVDMHAEATSEKMA
                     MGHYLDGEVVAVVGTHTHVQTADDQILPKGTAYLTDIGMTGPLHSVIGVKKELAIEKF
                     LTGMPRRFEVASGPSVFCAVLLELDVRLGKALSIERIRIID"
     gene            complement(3656..5233)
                     /gene="rny"
                     /locus_tag="VLL06_01880"
     CDS             complement(3656..5233)
                     /gene="rny"
                     /locus_tag="VLL06_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HSS29746.1"
                     /translation="MSPISTSIIAYILAGLVGALLGIGLFELIRRASSSAKKAQEVEQ
                     ARQAVQNAQREADNIVKEAKLEAKDLVLQSKAEIEREQKAKMAELAVAEKRVVQRDEA
                     LDKRMSAADKRDAEVQKRDLELVKREEKLTVKEAACTRVEKEHREALERVAGMTADEA
                     KKQLIQEMESQARLEAVGHAKRTLEEARENAEREAREIITSSIQRVVRDYVSESTISV
                     VPIPNDAMKGRIIGREGRNIRAIEAATGIDLIIDETPEAVIVSGFDPLRREIAKVSLE
                     RLMHDGRIHPTRIEEIVEKVKVDIDKLMYDEAEKIIFELGLSDFHPELIKVLGRLKYR
                     TSYGQNNLYHSREAAYICGIMASELGLDVKLARRGALLHDIGKAVSHEEEGPHAMLGA
                     EIAKKYGESEKIVNAIAGHHEQVEPICPESVLVAAAEALSAARPGARREALETYVKRL
                     EKLESLATTIKGVQKAYAIQAGREIRVIVRQEDITDAESFQLSRDLAKKIEQELTYPG
                     TIRVTVIRESRYVEYAK"
     gene            complement(5810..6106)
                     /locus_tag="VLL06_01885"
     CDS             complement(5810..6106)
                     /locus_tag="VLL06_01885"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HSS29747.1"
                     /translation="MTKTIDVEIYGQRYAIRGEADEADIRRLASFVDGQMRHLAEGMN
                     TTTPSKLAVLTALNLAHQLFDLERKRAQGEADVERRMKSLMESIEEQVPISLFR"
     gene            complement(6099..6353)
                     /gene="zapB"
                     /locus_tag="VLL06_01890"
     CDS             complement(6099..6353)
                     /gene="zapB"
                     /locus_tag="VLL06_01890"
                     /inference="COORDINATES: protein motif:HMM:NF017786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HSS29748.1"
                     /translation="MALDRLDALETRIRDLVKLVQELKKKNAAVEEELKVVRQRLAEK
                     DDSNRRWEQERVDIKSRIEKVLGDIELLECFEERKEVALD"
BASE COUNT         1469 a         2031 c         1473 g         1462 t
ORIGIN      
        1 ttgctgcaac acgttcaaca tcccaaggac attcacttgg gcatcgaata tcggatcttc
       61 tacagatttt cggacgtcca tctgtgcagc aaggtgcata ataatattcg gccgttcgtt
      121 gcgaaacacc cgttcaagtc gccaactttg aatatccagc ttgcagaaac gagcggcccg
      181 attcagattt ctccgttttc ctgtggacag attgtccacg acaacaactt cgtgcccttc
      241 ctgaataagg cgatcaacca cgtgtgaccc aataaaacct gcacctccag tcacgagtac
      301 cttcatgcat gcccccattg ccgatgagtc tgtgccatca tctgtccacc gtctacccga
      361 tcaggttgtg gcctcgcgta cgaatccaac aagaatctcc cgattccctt ttcgccctat
      421 cacaggagaa tcgagcacac ctttcaactg aagccccaaa tcagcggcac aggcgatgac
      481 cttctcagtc accgcctcgc gctgcgcatc atctcgcaca atgccccctc gccccacctg
      541 ccccttgcca acttcaaact gcggcttcac taatgccaca acctgtgcgg acctcttgag
      601 taggcgaacg accgcaggta ataccaatgt tagggaaata aaggacacgt cgaccaccgc
      661 cagatcgatt ggatcaggaa ccgctgcacg gtcaacatac cgaatgttag tccgttcgag
      721 caagacaaca cgagaatctt gccggagtct ccactcaaat tgtccatacc caacgtcgac
      781 agcatagatg cgggtggctc ctcgctgcaa cagacagtcg gtgaacccgc cggtcgaaca
      841 tccgacatcc agaccaatca gcccacgtgg atcaaaatga aaggcatcaa gcgccgcagc
      901 caatttgtca cctgatcgac tgacgaacaa cgcaggctgc acaacctcaa tccgcgcatc
      961 cagcgacacc aacttcgcgg gcttatccac catggtccca tcaaccgata ctccccccgc
     1021 caacaccgtt ctcactgcag cctccctgct tgacaccaag ccctggacca tcagcacacg
     1081 gtccagccgt tctttgtgag cacgcggggt ggaggccata gagattaggg aagcaacgat
     1141 tcagaacgca ggcagacaca agaaccagag tcaggaaagc taggaggaca gttcatcggc
     1201 ttcgccatct gacgtgtagg cgtggattcg ttttttgccg ttcttatctt gaacgagtac
     1261 ttctacttta tgttcggctt cttcgaggat cttgaggcaa ttttttgaaa ggcgaatacc
     1321 ctcttcgaag atcttgagcg attcgtccag aggaagatcg tttttttcga gctcaccaac
     1381 aataacttcc aaccgtgcca ttgcctgttc aaatttaacc gctgccacaa cacgcctcgt
     1441 aattagagtg tagagtccac attatactcg acgacatcac gatttcaaac tgatgaatcg
     1501 tcaaatatac gcgtgaccag acaactcaat cgcccgcttg ccaatcgagc ttccaactcc
     1561 tgtccaacct ctacatcttg ggctcggcga agaatctgac cagacgggac agtgtgaagg
     1621 atgctgtagc cacgcccaag aaccgccaat ggactcaggg tattgagttg cgccaacgtg
     1681 gcatggatcc tgtgacgatg ctgcgccaat agcaccccca tatacccttc caggcgcttc
     1741 gagaattgcg ggattgtcac cagaccttgc ttgatcgcga gaataggatt gagaccggaa
     1801 agatcgcgct gcacctcgtg aacccgttca cgcccggacg tgagcagttg ctgaaccatc
     1861 tcgcgcaatc gatccaccat atcatctgtc cgttgcgatg cttcctgaag acggaagcgt
     1921 atatctgtca cccctcgaat gcctgcctcg agccgctgcc gctcggtcgc acaatgctga
     1981 agcatcacct gtcctacacg gacctgtaac tctcggactc gttccacgat ctcagccaac
     2041 accggaacca cggtctccgc tgccgccgac ggcgttggcg ctctgaaatc ggcgacgaaa
     2101 tcggcaagcg tcacatcgac ttcatgaccg acagccgaga ccacagggat atgggaggct
     2161 gcaatggcac gcaccacgat ctcctcgttg aaactccaga gatcttccaa cgatcctccg
     2221 cctcgcccca caatgatgac atccacggaa tccagttcat tcaaggcagt cagcgcctcg
     2281 gcaatttgac gtccggcact ctccccctga acttgaaccg gagcgataag aatatggagc
     2341 gtgggccagc gtcgcctcag gacagcgaga atatcccgaa tggcagcgcc ggtcagcgat
     2401 gtgacgacac ccaccgtgcg aggaaatgct ggaatcgcct gctttctttc ttgatcgaac
     2461 agcccctcca ccgccagtcg ttcttttaat tgttcaaatg ccaactgcaa ggcgccgatt
     2521 cccttcggct ccaccctgtc gagtacgatc tgatattctc ctcgtggttc gtaaactgtg
     2581 agccgccccc gcacgagaac gtgaagccct tcttgcaagg aaaaacgcag ccgcaccgcg
     2641 ctggatcgga acagcaccgc gcgaatttgg cttgtcttgt ccttgagcgt gcaatagacg
     2701 tgtcccgatc ctggggcacg aaggttggag agctctccct ccaacaagat gtctgagaat
     2761 tgctcttcga ttgaggtgcg aagcagtccc gtcaactccg aaaccgtgaa gatccgtttc
     2821 ggaggatcga cctgagaggg ggaggcaggg aaaccggaaa ggctcgtggt cggtctcctt
     2881 taatcgatga tgcgaatgcg ttcgatcgag agcgccttcc caaggcgcac atcaagttca
     2941 agcagcaccg cgcaaaagac ggaaggcccc gaggccacct caaagcgccg gggcatcccg
     3001 gtcaggaact tttcaatcgc caactctttc tttacgccga tcacggaatg gagcgggccg
     3061 gtcatcccga tatccgtcag atacgcggtc cccttcggga gaatttgatc atcggccgtt
     3121 tgcacatgcg tatgggtacc gactacggca acgacctctc cgtccagata gtgccccatc
     3181 gccatttttt cggatgttgc ctccgcgtgc atgtcaacaa tcacagcagc cgttcgcttc
     3241 tttagcgctg ccagttcctt tttggctacc tggaacggac agtccaacgt cggcatgtac
     3301 gcccgcccca tcagctggag aacgccgagc ttctctccgc cggctgactc gaccaccaca
     3361 ctcccatgac ccggtacacc atccggatag ttagccggac gaagcaggcg tggttctcgc
     3421 gggaagtaat cgagaatttc ccttttgtcc cacgcatgat ttcctgtggt aatcacggcg
     3481 aggccaaggt cgaacaattc ttccgccaac tccggcgtga taccaaaccc gccggccgcg
     3541 ttctctccat ttccgatcac gatgtcgatt tgacgctgac ccaccagcct gggaacagcc
     3601 ctggccaatg ctcgacgccc aggctcccca aagatatcgc caataaagag caccttcact
     3661 tggcatactc cacataccga ctctcgcgaa tcacggtcac tctaatcgtg ccgggatacg
     3721 tgagttcctg ttcaatcttc ttcgccagat cccgagacag ttgaaacgac tcggcatcgg
     3781 tgatgtcttc ctgtcgaaca atgacccgaa tttctcgccc cgcttggatg gcataggcct
     3841 tctgtacccc tttgatggtc gtggcaagcg actccagttt ttccagccgc ttcacatagg
     3901 tctccaaggc ttcccgccta gccccgggcc tcgccgccga caatgcttct gccgctgcga
     3961 ccaaaaccga ttctggacag atcggctcca cctgttcgtg atgccccgca atggcattca
     4021 caatcttctc actctcgcca tacttcttgg caatctcagc acccagcatg gcatgcgggc
     4081 cttcctcttc gtgactgacg gctttcccga tgtcgtgcaa cagggcccct ctcctcgcca
     4141 acttcacatc gagaccaagc tccgaagcca tgatgccgca aatgtaggcg gcttcacgcg
     4201 aatgatacag attattttgt ccatagctcg tccggtactt gagccgaccg agcaccttga
     4261 tcaactccgg gtgaaaatct gagagtccca actcaaagat gattttctcg gcctcgtcat
     4321 acatgagctt atcgatatcg actttgactt tctcgacgat ttcctcgatt cgcgtcggat
     4381 ggatgcgccc gtcatgcatc aaccgttcaa gagagacctt ggcaatttct cgacggagcg
     4441 gatcgaaacc cgatacgatg acggcttcag gcgtctcatc gataatcagg tcgatcccgg
     4501 ttgccgcttc gatcgcccgg atgttgcgcc cttcacgtcc aatgatccgt cccttcatcg
     4561 catcattcgg aatcggcacg acagaaatcg tggattcgga aacgtaatca cgcaccaccc
     4621 gttgaatcga gctggtaatg atctcacgtg cttcccgttc cgcattctcc cgcgcctctt
     4681 ctaacgtccg cttggcatgc ccaacggctt caagccgtgc ctgactttcc atttcttgaa
     4741 taagttgctt cttcgcctcg tcagccgtca tccccgcaac gcgttccaac gcctcacggt
     4801 gctccttctc cactcgagta caggccgcct ccttcaccgt caacttctcc tcccgcttca
     4861 ccagttccag atctcgcttc tgaacctcag catcccgttt gtctgccgcg ctcatccgtt
     4921 tatcgagagc ctcatcgcgt tgaactacac gcttttctgc aacagcaagc tcggccattt
     4981 tagctttctg ttccctttca atctcggcct tcgactgtag cacaaggtct ttcgcttcta
     5041 gcttggcttc tttgactata ttgtcggcct cacgctgggc attctgtaca gcctggcgtg
     5101 cctgctccac ttcctgcgcc ttcttcgcgc tgctcgacgc gcgacgaatc agctcaaaga
     5161 gcccgattcc gagcaatgct ccaacgagtc ctgccaatat atacgcaata attgaggtcg
     5221 aaattggact caccccctta cagccgcgag acgagaagac atcggcttct cagccccgtc
     5281 ttaatacaag gttagcaacg gtcctatgag aagggagcgc agtggaaaga tgagagactt
     5341 agaagaggac agccagaaac acggtctaca gattactccg agaacggact ccacggcaat
     5401 gagtaccttg ctggattgag atagcgagca catcctgtgc gggacaaatc ccgcgaggtc
     5461 catctgcgtc ggcgcgcgct catccccatg agaggactcc gcgcacgaac cggaaacagc
     5521 tgttggccat ttccgcagac cgcactccct ggcggcaaca tgaactccat gaccttgccc
     5581 aacatctcca agaagtcata catgaggtgg acgaaccctt gtgcacattc taactgtctc
     5641 aatttcgcca gcatcaaaga cctatatcca ttccgtggtg actatgtcgt atccccggat
     5701 cactgaaccg gttcgagcgt tacctaacct aatccctatg gtagaatcca ctacgtcctc
     5761 acccattcga ttcacgataa caaagggaaa cgccctttgc aaggttaatc tagcgaaaga
     5821 gcgagatcgg tacctgttct tcaatcgatt ccatgagcga cttcatccgc cgctcgacat
     5881 ccgcctcacc ttgagcccgt ttccgttcga gatcaaacaa ttgatgggcc aaattgaggg
     5941 cggtcagcac agcgagcttg gagggagttg tcgtattcat cccttccgcg agatggcgca
     6001 tctggccatc tacaaagctc gcaaggcgcc ggatatcggc ctcgtccgcc tcaccacgaa
     6061 ttgcgtatcg ctggccatag atctccacgt cgatcgtctt agtcaagggc cacctccttg
     6121 cgttcttcaa agcactccaa cagctcaatg tcgccgagga ctttttctat acgagacttg
     6181 atatcgacac gctcttgctc ccatcgccga ttcgaatcat ccttctccgc aagtcgttgt
     6241 cgaacgacct ttaactcttc ttcaaccgct gcattctttt tcttcagctc ttgcacaagc
     6301 ttcaccaaat ccctaatgcg cgtttcgagc gcgtccagtc tatcgagcgc cattctctca
     6361 ctaccctcct taagaacccc ccacagtttg tggcgcacta tagaaacttg tctagccctt
     6421 ttcaataaat agcca
//
LOCUS       DATIPF010000073         5371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_631516, whole genome shotgun sequence.
ACCESSION   DATIPF010000073 DATIPF010000000
VERSION     DATIPF010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631516"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1641)
                     /locus_tag="VLL06_01895"
     CDS             complement(133..1641)
                     /locus_tag="VLL06_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSS29749.1"
                     /translation="MEQKLVDPAVPLAIPAASGGTVISRLFRTKSIEQLLADADHPDH
                     RLKKTLTAWDLTALGIGAIIGTGIFVLIGTAIVGDIHRPGAGPGIILSFVLSGLTCAL
                     AALCYAEFSTMIPVAGSAYTYSYATLGEFLAWITGWNLILEYGVACVAVAIGWSGYFN
                     NLLRLAGIELPHWATHPPGGPDGGVANFPAAIIVLLVTIILVIGIKESARATGVIVCL
                     KLAVILFFIAVGTPAVNTDNWTPFMPQGFAGVGAAAAIVFFAYIGFDAISTTAEEAKN
                     PQRDLPIGIIASLSICTVLYISVAAVLTGLIPAAQIDIHAPVAEALSMVGFKWGAAIV
                     AIGAVAGITSVLVVMMLGQIRVFFAMSRDHLLSPWLSTVHPRYGTPHHATILTGIGVA
                     ILAALIPIGDAADMTNIGTLFAFVLVCIGVVVLRYTKPNQLRPFRLPFMPLVPILGML
                     ACLGLMSFLPWLTWIRFGVWTAIGIVVYMGYGLRHSKLADSASESPLPKRSL"
     gene            complement(1796..2542)
                     /locus_tag="VLL06_01900"
     CDS             complement(1796..2542)
                     /locus_tag="VLL06_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HSS29750.1"
                     /translation="MKPIIGVTPDFNAGDRKEWGGREPTYFLRARYIRAIEELGGVPL
                     VLPLLADRATRRRLLQQIDGLLLTGSGPDLPPALYGERQRFPFEIVSDRRASFELDAV
                     HLAQQADVPLLAICGGMQAMNVACGGSLFQDIPSQISKPLQHRQEIPAIHLSHTVTVT
                     PGSLLRRIVRSVSMRVNSSHHQSVNVVAPSLVASALAPDGIVEAIESPTNRFFLGIQW
                     HPEFLFDRHPLHRRLFEAFLRASRATHRNA"
     gene            complement(2546..2992)
                     /locus_tag="VLL06_01905"
     CDS             complement(2546..2992)
                     /locus_tag="VLL06_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29751.1"
                     /translation="MNIYSPSSNRSRQTSILKLTAAFLLLALCTVSMVPSAWSQDGTV
                     PSSGTSTEGGTPSSAGMQVAAGFSTLLYLPVKVAFAIGGGIVGGLAYAFSGGNEQAAK
                     SIWDTSLYGTYLITPDHLQGNRPIRFLGVVDANDTPAPAPAPEPIR"
     gene            complement(3132..3596)
                     /locus_tag="VLL06_01910"
     CDS             complement(3132..3596)
                     /locus_tag="VLL06_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29752.1"
                     /translation="MTIEHAPEFSQFRVTLFVGPQPVEGRPFTSSCVFNVKKRSWKGG
                     IQVGVVITQSQVEMLSVGMDFSRWLAQALIGLSDEDRLSHQERAHELFIQAVCWCKLD
                     LALQAGITQENQFLADETWTAQVRDTVIKRTNFVTSYVASELDLVLKDLTAP"
     gene            3676..4875
                     /locus_tag="VLL06_01915"
     CDS             3676..4875
                     /locus_tag="VLL06_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HSS29753.1"
                     /translation="MMTTAVPQRVSEIQQALRDAEIDGWLFYDFRRSDPLAYRILQLD
                     PTLHVTRRWYYWIPAEGMPVKLLHRIEPHVLDALPGQAHSYVSWEQQRQILGRLLAGG
                     LRVAMQYSPLNAVPYVSRVDAGTIELIRSYHVEVVSSADLIQTFEARWTDGQLESHQF
                     AAAALRRIVDQTFSHVHEEITQGRGLTEYGVQQFILARIREAGMVTSSAPIAAVNAHS
                     ADPHYGPPETGSVEVTRDALLLIDLWAKRAEPGSVYADITWTAYVGQTVPVKHREIFD
                     LVRQGRDAALTFVRTEIAAGHRPFGWEVDDACRQVIQQSGYGDQFVHRTGHSIGEEVH
                     GNGANIDGLETQDTRRLMPRTCFSIEPGIYLPGEFGIRSEMDVYLAEREALVFGLPLQ
                     SEIVSLF"
     gene            5168..5293
                     /gene="tatA"
                     /locus_tag="VLL06_01920"
                     /pseudo
     CDS             5168..5293
                     /gene="tatA"
                     /locus_tag="VLL06_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
BASE COUNT         1206 a         1426 c         1636 g         1103 t
ORIGIN      
        1 ctgctccaac cttggtacct gagccagcag acacatcaag ctaactcact tgagctagca
       61 ggctgtggaa aagttctgtg agtacactgg aacttccctg acctacatgt ctgtgataac
      121 agaagcaccg tgttagaggg agcgttttgg aaggggactt tcagaggcgg agtcggccag
      181 tttgctgtgc ctcaggccat accccatgta gaccacaatg ccgatggcag tccagacgcc
      241 gaatcgaatc caggttaacc atgggagaaa gctcatgagg ccaaggcagg ccagcatgcc
      301 cagaattgga acgagcggca tgaacggaag ccggaatggt cgaagttggt tgggtttcgt
      361 gtaccgtagg actacaacac cgatacaaac aaggacaaaa gcaaagagag tgccgatgtt
      421 cgtcatgtcc gctgcgtcac caataggaat cagggccgcg agtatcgcca cgccgatacc
      481 ggtgagaatg gtggcgtgat gaggcgtgcc atagcgcgga tgcacagttg acagccaagg
      541 gctcaagaga tggtcgcggg acatggcaaa gaagacccgg atctgaccca gcatcatcac
      601 gacgagtacg ctggtaattc cggccacggc tccaatcgcc acaatcgccg ctccccattt
      661 gaagcccacc atactcaacg cttcggccac cggcgcatgg atgtcgatct gtgcagcggg
      721 aatcaggccg gtcaggacag cggctacgga gatgtagagc acggtgcaga tgctgagtga
      781 ggcgataatt ccgatcggca gatcccgttg agggtttttg gcttcttcgg ccgtcgtcga
      841 gatggcgtcg aatccaatgt aggcgaagaa tacgatggcc gccgcggcac caacaccggc
      901 gaatccctgc ggcataaagg gcgtccagtt atccgtattc acggccggcg tccccacggc
      961 aatgaaaaag agaatgaccg cgagtttcaa gcacacaatg acgccggttg cgcgtgcgct
     1021 ttctttgatt ccaatgacga ggatgatggt gacaagtaac acgatgatgg cggcgggaaa
     1081 gtttgccacg cccccgtcgg gtcctccggg tggatgcgta gcccagtgcg gcaattcgat
     1141 cccggccaat ctgagcaaat tattgaaata gccggaccat ccgatggcga cggcgacaca
     1201 ggctacgcca tattcgagaa tcaaattcca accggtgatc caggcaagga attctcccaa
     1261 ggtggcatac gagtaggtat aggcggagcc ggccacggga atcatggtgg aaaactctgc
     1321 atagcagagt gccgcgaggg cgcaggtcag accggaaagg acaaaagaca aaatgatgcc
     1381 aggcccggct cccggccggt ggatatcgcc gacgatggcg gtgccgatca ggacgaagat
     1441 gccggtgccg atgatcgcgc caatgccaag ggccgtgaga tcccaggccg tgagggtctt
     1501 ttttaaacga tgatcgggat gatcggcgtc cgcgaggagt tgctcgattg atttggtccg
     1561 gaagagccgg ctgatcactg tacctccgct ggccgcgggg atggcaagag gtacggcagg
     1621 gtcaaccagc ttctgttcca tatggggtca agtatagcag gcctcctgcg gaggataatc
     1681 gaacaagact ggcgggaccg gttgatctgg ctcatctggt tcgtttcgtt cacccggtta
     1741 gtccgctcaa ccaaccaaac caaatacaca agagaaatga gccaggtctc gcgcgtcagg
     1801 cattgcggtg tgtcgcgcgg gaggcgcgca gaaaggcctc gaataatctt cgatggagag
     1861 ggtgccggtc aaagagaaat tccggatgcc actggatgcc cagaaaaaag cggttggtcg
     1921 gagattcgat tgcctccacg ataccatcgg gggccagggc gcttgcgact aatgacggcg
     1981 cgacgacgtt gaccgattga tggtgcgagc tattcacccg catggacacc gatcgcacga
     2041 tgcgacggag gaggctgccg ggtgtgacgg tgacggtgtg actgaggtgt attgcaggaa
     2101 tctcttgacg atgttgaagc ggctttgaga tttgggacgg gatatcctga aacagactgc
     2161 cgccgcaggc gacgttcatg gcctgcatgc ctccgcagat ggcaagcaac gggacatcag
     2221 cctgttgtgc caaatggacg gcgtccagtt caaaactcgc acgacgatcg ctcacgattt
     2281 cgaacggaaa gcgttgacgt tcgccgtaca acgctggtgg aaggtccggc ccgcttccgg
     2341 tgaggaggag tccgtcgatc tgctgcagga ggcgccgtct cgtggcccgg tctgcgagga
     2401 gtggaaggac cagggggacg cccccgagtt cttcgatggc acggatgtag cgtgcgcgca
     2461 aaaaataggt gggctcacgc ccaccccatt cctttcgatc gccggcgttg aagtctggtg
     2521 tgacgccgat gatcggtttc atcggttagc ggatgggctc cggagcaggg gctggcgccg
     2581 gtgtgtcgtt cgcatcaacg actcccagga agcggatggg tcgattacct tgtaggtgat
     2641 cgggggtaat gaggtaggta ccgtacagac tagtgtccca gatgcttttg gccgcctgtt
     2701 cattcccacc agaaaacgca taggcgagcc ctccaacaat ccctcctccg atggcaaagg
     2761 ctactttcac tggaagatac aagagggttg aaaaaccggc agcgacctgc atacccgcgc
     2821 tcgacggggt tccgccttca gtgctcgtcc ctgacgaggg gaccgtgcca tcttggctcc
     2881 acgccgaagg caccatgctg acggtacaca gggccaggag caagaaggct gccgtgagtt
     2941 tcaagatgga agtctgacga gagcgattgg acgaaggcga atatatgttc acaggtcatt
     3001 ctcctttttc aacagagcga gctgaacggc agtggttgac tccagagcgc tcacccacga
     3061 tagagtggta gaggggttat agcaaattca actgttgttg caaaccccct gccgcgtttg
     3121 catgaagggt cttatggagc ggtcaagtct ttcagtacga ggtccagttc agatgcaaca
     3181 tacgacgtga cgaagttggt ccgcttgatg acggtatctc tgacctgggc agtccaggtc
     3241 tcgtctgcga gaaattggtt ttcttgagtg atgccggctt gcaacgccag gtccagcttg
     3301 caccagcaga cggcttggat aaacagctca tgggctcgtt cctgatgaga gagacgatct
     3361 tcatctgaca gaccgatgag ggcctgggcc aaccagcggg agaaatccat gccgacgctc
     3421 agcatctcaa cctgactctg cgtgatcacg acgccgacct ggatcccacc tttccaactc
     3481 cgtttcttga cgttgaatac acaagaggaa gtgaagggtc ttccctccac aggctgcggt
     3541 ccgacaaaca gggtcacgcg gaactgggaa aactctggtg catgctcaat ggtcatggtg
     3601 cgcattgtag catggccacc gatctccgcg gtgggctcat tgacggcttc ctagcgtgac
     3661 gataagatgc cattcatgat gactacggct gtaccgcagc gtgtcagcga gattcagcag
     3721 gcacttcgtg atgccgaaat tgatggctgg cttttctatg atttccgcag gagtgacccc
     3781 ctcgcctatc gcatacttca gctcgatcct accctccatg tgacgaggcg atggtactac
     3841 tggattccgg cagaggggat gcccgtcaag ctgctgcatc gcatcgagcc gcatgtgctg
     3901 gatgcattgc caggacaggc acactcgtat gtgtcgtggg agcagcaacg gcagatcctt
     3961 ggacgcctcc tagccggtgg cctccgcgtc gcgatgcagt attccccgct gaatgcagtc
     4021 ccctacgtgt cacgcgtgga tgccggtacg atcgagttga tcagaagtta ccacgtcgag
     4081 gtggtcagtt ctgctgattt gattcaaacg tttgaagcac gctggacgga cggtcaactc
     4141 gaatcgcatc agtttgctgc tgcggcgctt cggcgcatcg tcgatcagac atttagccat
     4201 gtacacgagg agatcacgca agggcggggt ctcactgaat atggcgtaca gcagtttatt
     4261 ctggctcgca tacgcgaagc agggatggtc acgtcgagtg cgccgatcgc cgccgtgaat
     4321 gcccacagcg ctgatcccca ttatggcccg ccagagacgg ggtctgtcga agtgactcgc
     4381 gatgcgttgc tcttgatcga tctctgggcc aaacgagcgg agcccggctc agtctatgcc
     4441 gatattacct ggactgccta tgtcggtcag accgtgccgg tcaagcaccg cgagattttc
     4501 gacttggtca ggcagggacg ggatgcggcc ttgacctttg tccggacaga aatagctgca
     4561 ggccatcgcc cgttcggctg ggaggtcgac gacgcctgcc gtcaggttat ccaacagagt
     4621 ggctatggtg atcaatttgt ccatcgcacg ggccattcga tcggcgaaga agtccatggc
     4681 aacggagcca acatcgatgg cttggagacg caggataccc gccgtctgat gccgaggacc
     4741 tgcttttcga ttgagccggg gatctacttg cccggcgagt ttgggatcag gagcgagatg
     4801 gatgtatacc tcgctgagcg tgaggcgttg gtgttcgggc tgccgttaca gagcgagatc
     4861 gtatccctct tttagccggg tgctgaaaaa ggctgccagc ggcgttctcc ccttgaaagc
     4921 atcctcaacg tagccctcgc ggaggacaag ctgtcttggc agcttggggt gggcgggtga
     4981 gaagaagtac gcctccggtg ctttcatcgg ctgcggcctt gctggacagc cttttttgca
     5041 cccggctaga atatattgtc ccttccttga cagtgcctca tgatggtcta tagagttaat
     5101 tcgactctca atacggcaaa gctaatggcg ggtgtcggtc acgactccgg gtaaaaggaa
     5161 tcacgccatg tttggtacca tggggatctc ggaactcatc attatcctcg tcattgtcct
     5221 catcattttt ggggcgggaa agctcccgca aatcggtgaa ggagtcggca aagcgctcag
     5281 ggggtttaaa aaagaagtca acgatattcc tccgccggaa gcggcaccgt ccgaggcgaa
     5341 tcaaccagca cctgtgcaga tgcaatcaac a
//
LOCUS       DATIPF010000074         5114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_809091, whole genome shotgun sequence.
ACCESSION   DATIPF010000074 DATIPF010000000
VERSION     DATIPF010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809091"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            739..1407
                     /locus_tag="VLL06_01925"
     CDS             739..1407
                     /locus_tag="VLL06_01925"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSS29754.1"
                     /translation="MKKRTTQPTPRNGQRASAHDRQASLIAAAASLFAAKGFNGTTTK
                     EIAKAAGVSEALVFKYFPTKRTLYGAILAEKVTINELLEPVEAAAKKRDDHRVFTLIA
                     SYRIRPGVDSTLLRLLLFSALEGHELSEMFFGKHHKVFYDHLAAYIRTRIEDGAFRPI
                     DPLLAARAFIGMIAHHRLLHEIFSVPMHQSHEHTVATYVDLFLTGLVKMPADCQKTPG
                     RPVR"
     gene            1404..2594
                     /locus_tag="VLL06_01930"
     CDS             1404..2594
                     /locus_tag="VLL06_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSS29755.1"
                     /translation="MNPLKQHPIVTLGVIIFFAITALVVFRLSSGAKSDTRKARAITV
                     STVAPLKQDLDIRLTYTADISPNQTVNLFSRVDGYIAKIYVDKGDLVKTNQLLIEIDH
                     TDYQHAVNQSKANLAAARARVAQQDASVRNATLTLTRMQTLIKDQFVSQQDLDNAQVA
                     FDAAAAALDSLRAQVQQMEVAQAQAETNLAYSFIRAPFAGYVAERNLDLGSYVSGTTA
                     GTSTVSRGILTLHEIQTVRILIEVVEKDVPLIHVAQKAEVRAEAYPDRVFEGTVTRIV
                     QALNRATRTMTIEVDLPNKDHVLKGGMFARVEILVGTHPHAIQIPIDAVSRLEDVQYV
                     YIVREGKAQRVPVEIGFRDENRVEITKGLDGSEQVIVSGKDLVHEGSPVQAQPLVAPM
                     KREG"
     gene            2684..>5114
                     /locus_tag="VLL06_01935"
     CDS             2684..>5114
                     /locus_tag="VLL06_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSS29756.1"
                     /translation="MFVFIMWLTLLALRNRIGILMLSLAMVILGGTSVQRIPVDLFPQ
                     IQVPVAFVGVIYKGAPPLDIEQSVVYPIEKAVSSASNVEHVESFAKQGIGAVQIWFNW
                     GTDINAGQMEVMQRVTQILNSLPPGIGQPFIVKFDVSNIPVSLVTVSSDDLDERALYD
                     LASNTIAPQIEQIANVAAATVEGGKIRQININLDPALLNARSLSILDVVKSVKAANLI
                     LPSGDIKAGNLDYNVFTNNQFRTVDPIQDVIVKVNPQGSPVRVRDVGTVTDSSDIQTN
                     IVRTDGARAVYLRVNKQPIANTVGVVDALRKALPKMVGIPPSVKIGISFDQSIYIRQS
                     ISNLIEQALHGSLLAALVILMFLRNLTSTLIISVAIPLSIMVTFIVLYFSGQTLNVFT
                     LGGLALGIGRLVDDSIVELENIQRHLNTTSRRWTAILEAAREVAMPILASTVTTVVVF
                     LPIFFVAGIARLLLIPLTITIAISLFTSFFVSRTVTPALCYKFLKPEQESHRSMPPWF
                     VRTMDWSRERYESLDKGYEESLRWVLAHRWLLISGILLFFAASLALALKIGTEFLPVS
                     DESQFRIVLRGPVGQRVEKTEQQVAEVERVLRAQIPAEELETIVSSTGVLAQGRSSLF
                     NPNTGPHTSAISVHPVTPDKRKRNQVQIMNDVRPKVLALFPGVAMYFDPGGIIKRITS
                     FGSQKSVDVEIYGYEFDKAREVIRQVESLMHQIPGLADIEVSREENYPEVNVVVDREK
                     AALLGISETDVATAVLFSLNGNGQTDPIIYTDPQNGNEYYISAWLAEEHRKDLTAIEN
                     IILIARTGEPVL"
BASE COUNT         1146 a         1608 c         1325 g         1035 t
ORIGIN      
        1 ttagagcgac tgtgttgcac ggagtaccca atggaccgga ctacgatgga ccgtcaaaaa
       61 cgtggcaacc cattcgtcag ttcagacccc agacggcagg gcggctcagg gatcccttcc
      121 agattctggt gtgtgtctgt cgctggtagt ttcctgcgtg ttggcggcgg caaatgatcc
      181 gcaggcatct agatcgtact cgactgcaac tgaatttagg cacctcctgt caagataacc
      241 ctcagctcca cagcctcata gaacagtacg cctcctagca ggccaggatt tgttccaact
      301 cttgttctcc ctcctattaa gagagctgaa acgagctgga tcgatcaagg atggatgggg
      361 tagaagccca gagcctctac ctcccccgtt ctcaacgatc atattatccc agtccgtaaa
      421 tccgccttag tggaaatccg cgctcatgcc gccatgcaca caccataata cgctgctcga
      481 aggacttgct gacacctgcc gcaccaccca tgttgaaagc tcacgcccca catctgggca
      541 gggttctccg caaaagccga tctcgaccga tagttacccg cctgtatagc gtgaaataga
      601 tgtccctgtg gaagatcagc gagggccaca gacggatcaa ccggactcgc cctgaaacgg
      661 ctcacgtcgt ttctcaccca tgcttgccgc ctagaaacgg tgcatgctag gatctcggta
      721 agtgagcact tacttaccat gaagaaacgg accacacagc cgacgcctcg gaatgggcaa
      781 cgggcatctg cgcacgatcg tcaggccagc ctcattgcgg ccgctgcttc gctcttcgcc
      841 gcgaaagggt tcaacggcac caccaccaaa gagattgcca aagcggcagg cgtaagcgag
      901 gccttggtct tcaaatactt tccgacgaaa cggacgctct acggcgcaat tctggcagaa
      961 aaagtaacca tcaacgagct actcgaaccg gtggaggcag cggccaagaa acgcgacgac
     1021 catcgcgtgt ttacattgat cgcgagctat cgcattcgcc ccggggtcga ctcgacactc
     1081 ttgcgactcc tcctgttcag cgcgctggaa gggcacgaac tgtccgagat gttcttcggc
     1141 aagcaccaca aagtcttcta tgaccatctc gccgcataca ttcgcacaag aattgaagat
     1201 ggcgcgtttc ggcctatcga tcccctcctg gctgcccgcg ccttcatcgg catgatcgcc
     1261 catcaccggc tgctccatga gatattcagc gtccccatgc accaatcgca tgagcatacg
     1321 gtggcaacct acgtcgacct gttcctcacc ggactggtca aaatgccggc ggactgtcag
     1381 aagacaccgg gaaggcctgt gcgatgaacc ccctgaaaca acatccgatt gtcacactcg
     1441 gcgtcattat tttctttgcc atcacggccc tcgtcgtgtt tcgcctcagc agcggggcca
     1501 agagcgacac ccgcaaagcc agggccatca ccgtctccac cgtggcaccc ctgaaacagg
     1561 acctggacat tcgcctgacc tataccgccg acatcagccc caaccaaacg gtcaatctgt
     1621 tctcacgggt tgacggttac atcgccaaga tctacgtcga taaaggcgac ctcgtcaaaa
     1681 ccaaccagct gctgatcgag atcgatcaca cggattatca gcatgccgtc aaccagtcca
     1741 aagcaaacct cgctgcagcc agagccaggg ttgcgcagca ggacgcgagt gtccgcaatg
     1801 ccaccctcac gctcactagg atgcagacgt taatcaagga tcagttcgtc tcgcaacagg
     1861 acttggataa cgcccaggtt gcgtttgatg cggcagcagc ggcactcgac tcactccgcg
     1921 cacaggtcca gcaaatggag gtcgcccagg cacaggccga gacaaatctg gcctactcat
     1981 ttatccgtgc acccttcgcg ggttacgtgg ccgaacgcaa cctcgatctg ggctcctatg
     2041 tcagcgggac aaccgccggc acctccaccg tctcgcgagg aattctcacg ctccatgaga
     2101 tccagacggt ccgcattctg atcgaggtcg tcgagaagga tgttcccctc atccatgtgg
     2161 ctcaaaaggc agaagtgcgt gccgaggcct atcccgatcg cgtgttcgag ggcacggtca
     2221 cccgcatcgt ccaggcgctg aaccgggcca cccgcacgat gacgatcgag gtggatcttc
     2281 cgaacaagga tcatgtcttg aaaggcggca tgttcgcccg cgtcgagatt ctcgtcggca
     2341 cgcatcccca cgcgattcaa atccccatcg acgcagtgag tcgattggaa gatgtccaat
     2401 atgtctacat cgtgcgcgaa ggaaaggccc agcgggtgcc ggtggaaatc ggattccgcg
     2461 acgaaaaccg ggtggagatt accaagggat tggatgggtc ggagcaggtg attgtctccg
     2521 ggaaagacct cgtgcacgaa ggcagccccg tgcaggctca acctcttgtt gcacccatga
     2581 agcgtgaggg ttgacacgtg aggtgtgcgg tgaagacttc gtaccgctgt ccacgtctct
     2641 gttcagccca tgacctccta cgccagacat tccacgattc acgttgttcg tcttcattat
     2701 gtggctgact ctgctcgcac ttcgcaaccg catcggcatc ctgatgctgt ccctggccat
     2761 ggtgatcctg ggcggcacct ctgtgcaacg gataccggtc gatctgtttc cacagattca
     2821 agtgcctgtt gcgttcgtcg gcgtgatcta taaaggcgca ccaccgctcg acatcgaaca
     2881 gagtgtcgtc tatcccatcg agaaagctgt cagttctgcg tcgaacgtcg agcatgtcga
     2941 gtccttcgcc aagcagggca tcggcgcagt acagatctgg ttcaactggg gcacggacat
     3001 taacgctggc cagatggagg tgatgcaacg tgtcacccaa attctcaata gccttccgcc
     3061 cggcatcggg caacccttca tcgtcaagtt cgacgtctcc aatatccctg tctcccttgt
     3121 gacggtctcc agcgacgatc tcgacgaacg ggccctctac gacttggcct cgaatacgat
     3181 tgccccacag attgaacaga tcgccaacgt cgccgcagct accgtggagg gagggaagat
     3241 ccgtcagatc aacatcaacc tcgacccggc actgttaaat gctcgcagcc tctcgatcct
     3301 cgacgtcgtc aaatccgtga aggctgccaa tctgattctc ccctcgggcg acatcaaagc
     3361 cggcaatttg gactacaacg tcttcaccaa taaccagttc cgcacggtcg acccgatcca
     3421 ggacgtgatc gtgaaggtca acccgcaagg cagccccgta cgcgtacggg acgtcgggac
     3481 cgtgaccgat tcgtcggaca ttcaaaccaa catcgtgagg accgacgggg cccgagccgt
     3541 atatctgcgg gtgaacaaac aacccatcgc caacaccgtc ggggtggtgg acgcgctgcg
     3601 caaagctctc ccgaagatgg tcggcattcc tcccagcgtg aagattggca tttcgttcga
     3661 ccaatcgatc tacattcgcc agtcgatcag caacctcatc gaacaggccc tgcacggatc
     3721 gctgctagcc gcattggtca ttttgatgtt cctccgtaat ctcacgagca ccctgatcat
     3781 ctctgtcgct attcccctct cgatcatggt gaccttcatc gtgctgtact tctccggaca
     3841 aaccctgaat gtgttcacat tgggggggtt ggccctcgga atcggccgcc tcgtcgacga
     3901 ttccatcgtg gaactcgaaa acattcaacg gcatctcaac acgacgtccc gccgctggac
     3961 cgcgattctg gaagcggccc gcgaagtggc catgcccatt ctcgcctcga cggtcaccac
     4021 ggtcgtcgtg tttctcccaa ttttcttcgt cgccgggatt gcccgcttgc tcctgattcc
     4081 cctgactatc acgattgcca tctcgctgtt cacatccttc ttcgtgtcac gcacggtgac
     4141 cccagccctt tgctacaaat tcctcaagcc ggaacaggag tctcaccgtt cgatgccgcc
     4201 atggttcgtc cgtacgatgg attggagccg cgaacgatac gagtccttgg acaaaggcta
     4261 tgaagaatcg ctacgctggg tcttggccca tcgctggctc ctgatcagcg gcattctgct
     4321 cttcttcgcc gcctcgctcg cgctggcgct caagatcggt acggagttct tgccggtgtc
     4381 ggatgaaagc cagttccgca tcgtcctgcg cggtccggtc ggtcagcggg tggaaaaaac
     4441 cgagcagcaa gtggccgaag tcgaacgggt actgcgggca cagatccctg ccgaagagct
     4501 ggaaaccatc gtgtccagta ccggcgtgtt agcacagggc cgatcctccc tgttcaaccc
     4561 gaacaccggc ccccacacat cagccatttc cgtccatccc gtcaccccgg acaaacggaa
     4621 gcgaaaccaa gtacagatca tgaacgacgt gaggcccaaa gtcttagccc tatttccagg
     4681 cgtcgcgatg tatttcgatc cgggcgggat catcaagcga atcaccagct ttgggtcgca
     4741 gaagtctgtc gacgtcgaaa tctacggcta cgaattcgac aaagcgcggg aggtcatccg
     4801 gcaggtcgaa agcctcatgc atcaaatacc gggcctcgcc gacatcgaag tcagccgcga
     4861 ggagaactat cccgaggtca acgtggtagt ggatcgcgag aaggccgctc tccttggtat
     4921 cagcgaaacg gacgtcgcta ccgccgtgct cttctctctc aacggcaacg ggcaaaccga
     4981 ccccatcatc tacaccgacc cccaaaacgg gaacgagtac tacatcagcg cctggctcgc
     5041 ggaagaacac aggaaagacc tcaccgcgat cgagaatatc attttgatcg cacgaaccgg
     5101 agaaccggtc cttc
//
LOCUS       DATIPF010000075         2712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_897615, whole genome shotgun sequence.
ACCESSION   DATIPF010000075 DATIPF010000000
VERSION     DATIPF010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897615"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VLL06_01940"
     CDS             complement(<1..556)
                     /locus_tag="VLL06_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29757.1"
                     /translation="MSRIKELRASFDKKLDALEHQALALEAQLTHTKDQAMQRLEQRK
                     QQLQDLLKQVQADVQKSKEMAEQAKTDVQGRIEHLQVQLALGKADARDTFEEQRTKIL
                     KALNEFESVADQRLKGAGFESGRIWEDLVGRTSSLEAEFEAVKGRFELEKAKQQGMLE
                     SKKQELLAELVLFKDQLRAKRGAVQ"
     gene            1864..2547
                     /locus_tag="VLL06_01945"
     CDS             1864..2547
                     /locus_tag="VLL06_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HSS29758.1"
                     /translation="MVFKNREEAGRRLGVELMHHRDDPGGLILALPRGGVAVGYQLSL
                     ALHFPLDVFLTRKIGAPGNPEYAMGAVAEAGSRYVNQEVVDALGVSRHELERLIQAQE
                     KEIARRKELYRQGRPLPQLTGRTVFLVDDGIATGSTFMASALAIRSLQPRRLIGVIPV
                     GPSSTIREIRTHVDELAVLMTPDPFEAVGNFFVDFTQVDDRLVMEYLHLAEEAMLERT
                     PPSPASSDV"
BASE COUNT          559 a          800 c          715 g          638 t
ORIGIN      
        1 cctgtaccgc cccgcgcttg gccctgagct gatccttgaa caacacgagt tcggcaagca
       61 gctcttgttt ctttgactcc agcatgccct gttgcttggc cttctcaagt tcaaaccgcc
      121 ccttgactgc ctcaaactcg gcttcgaggc tgctcgtccg ccccacaaga tcctcccaga
      181 tcctgcccga ttcgaacccg gcccctttca gcctctggtc cgcaacggat tcaaactcat
      241 tcagggcctt gagaatcttc gtcctctgct cttcgaacgt atcgcgggcg tctgctttgc
      301 cgagagccaa ttgcacttga agatgttcaa tcctgccctg cacgtcggtc ttcgcctgct
      361 cggccatctc cttcgacttc tgcacgtcgg cctgaacctg cttgagcaga tcttgcagct
      421 gttgcttgcg ctgctccagc cgctgcatcg cctgatcttt cgtgtgggtc aattgcgctt
      481 ccaacgctag cgcctggtgc tccaaggcat ccagcttctt gtcaaacgac gctctgagtt
      541 ccttgatgcg cgacatggga ccctcctcta tgtgagatat gtgagatgga acatataact
      601 gccgaatgtg gcatgacatc atcaccgaat agaagaccga atcctggact tcacccgctc
      661 gattctctct tcatgatcgt ccgtgctatt cgactccgaa aggaccatca caagcgctct
      721 caacgactgc cgacagttga acctcttcga ccagaagttt ctgtattgtt gcacaggctt
      781 cacggaagcc tctcccggtt cggtcggctg cctgatgata ggctatgttc ccgcggccag
      841 agtgtcaatc gtgctgatgt cgccttcaac cagacggctt gtctgtctct atcggtatcc
      901 gcaggggcga catcgctcag gttcccaagt tttctgcctt gctttgaaga aatcagagaa
      961 ttacgatagt ggagatctgc tagcgaatcc tcttcgccgt tcgagaggtg cacgatggac
     1021 ctcgaagaat caggacgaat tgcacaacca cccccaatga ctccaccctg tgcctgtcta
     1081 cacagtcgcc tgatctccga ttgcgtctcg gaggaggaac acaaagccgg caaggtacgt
     1141 tgtgtggaat gcggggacat cattttcgat ccccatctcc aacacagagc caagggagcg
     1201 tagtccctct tcctatcaat gttgcgtccc tcagattctg ctgaagaaac actgtaccgt
     1261 tcccttcact gaggtatttg gcaacagttt caggactagt cactgacccg aaattcctca
     1321 accggctgaa tagtgcatcc agctccttca tgctgtacgt ctgtaacccc atgtggtacc
     1381 gcgcgtctcc cgctaccgga cgacgacacc atcctgtggc accgacgatg cagggtctta
     1441 ttcagcaagg ccgaatccac cgtcaaccca ggaggatatg atggaaattg ccatcgccat
     1501 aggcggccta gccgccgtat tcgtggggct gggggctctg attttgttta caccctatga
     1561 caaatgacga cgcatacaga atgagtgcct ttccattctt attgatgggg aggttccatc
     1621 ttcatcacaa ggagattctt gaggagaacg gaagggtcgt gtgcgatgct ccgacatcct
     1681 catgatcttg aggttccgtg ggacatgcga tcgtatcgcg tgtccacggg gcctcactgt
     1741 cctccatagt gacggatcgc gctgctggta ttgctggcgc tgtttctgat cacggtcgac
     1801 atcacgaatg gacgcacccc gcatcacagg ttgtaagacc tatcatatga ggaaggagta
     1861 tcggtggttt tcaaaaaccg tgaagaggca ggccgtcggc ttggcgttga gttgatgcat
     1921 catcgtgacg accccggggg tctcattctg gcactgcccc ggggtggcgt ggcggtggga
     1981 taccaactca gcctcgccct ccatttcccg ctcgatgtct ttctcacacg caagatcggc
     2041 gcgccgggca atcccgagta tgcgatgggg gctgtggccg aggcaggatc ccgctatgtg
     2101 aatcaagagg tggttgacgc gctcggtgta tcccggcatg agctggaacg gttgattcag
     2161 gcccaagaga aagagatcgc caggcgcaag gagttgtacc ggcagggacg gccgctcccg
     2221 caactcacgg gccgcaccgt gttcttggtt gacgacggca tcgccacagg ttcgactttc
     2281 atggcctcgg cacttgccat acgcagttta caacctcgcc gtctgatcgg ggtcattcca
     2341 gtaggaccct cgtcgacgat ccgtgaaatc cgaacacatg tggacgagtt ggcggtgctc
     2401 atgacaccgg atccgttcga ggccgtggga aactttttcg tggacttcac gcaagtcgat
     2461 gatcgccttg tcatggaata tttacatctg gccgaagagg ccatgctcga acgcacgccc
     2521 ccgtcaccgg cctcatcaga cgtctagtca gcagaggaga gtgcgccata acatgcctgc
     2581 acaatccagc atcccgtcgg agttgggggc tcaaagagcc cacgcgtact tcgatgacga
     2641 acacagggag gcgaccatga gaatcttagt cggcatcgat tggtcggacg aaccctttgc
     2701 taccgtggaa ca
//
LOCUS       DATIPF010000076         1609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_190163, whole genome shotgun sequence.
ACCESSION   DATIPF010000076 DATIPF010000000
VERSION     DATIPF010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190163"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..949)
                     /locus_tag="VLL06_01950"
     CDS             complement(272..949)
                     /locus_tag="VLL06_01950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HSS29759.1"
                     /translation="MTPSKKKNAPPSWRHYNFTTAGPVDDYLYSMLPKREAVLSEMEE
                     YASEHNIPIVGPAVANVLQQLALMIKARTVFELGSAIGYSTIWWAQAVGEGGRVIYTD
                     GDSKNAERARGYFERAGVANRITVHVGDALEVLSEQKQEFDVIFNDVDKDDYPRVLRL
                     VAPRLRKGGLFITDNVLWSGRVAEKNPTDGRTKAILEFNRKLYDANEFFTTILPIRDG
                     LAIALKK"
     gene            complement(985..>1609)
                     /locus_tag="VLL06_01955"
     CDS             complement(985..>1609)
                     /locus_tag="VLL06_01955"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HSS29760.1"
                     /translation="IDPWIMGNPMCPDSEKNVKRVDILFCTHGHGDHIGDAVEICKKH
                     NPIVVGIPELARWLGKKGAKQIAEMNKGGTQTVGDIKVTMVHADHSCGIQDGDETIYG
                     GEACGYVIEFSSGQKIYHAGDTNVFGDMAIIRDLYAPDVVMLPIGDHYTMGPREAAYA
                     CNLLKPKAVIPMHFGTFTVLTGRPNQLQKLVPGVEVVEMKPGQTIGG"
BASE COUNT          364 a          468 c          398 g          379 t
ORIGIN      
        1 cgatgaccag cacaattccg aagacgatca tgccgatcct gccaggagaa cgcgatatct
       61 tcgtgtcgag tgctgttgag gccgtagcca tagttaactc cgctggatgc tcaagagagt
      121 aacgctgagc tccagatcaa tttgggcaat atagagcagg aatgttacac ggaggttaaa
      181 acaggctgcc tctcgatgga attctcacta tgcggcaaac tcaaacaaga ctggcccgcc
      241 tcagttggga atcagcgtga ttgcgccaaa gctatttttt cagtgcgatg gctagtccgt
      301 cgcggatcgg cagaatcgtc gtgaaaaact cgttcgcgtc gtacagcttc cggttgaatt
      361 ccaggatcgc cttcgtacgg ccatcagtcg gattcttctc ggcaactcgt ccgctccaga
      421 gcacgttgtc tgtgatgaaa aggccaccct tccgtagacg aggagccacc aggcgcaaca
      481 cgcgagggta atcgtctttg tcgacatcat tgaagatgac gtcgaactcc tgcttttgtt
      541 cggagagaac ttccaaagca tcgccaacat gcacggtgat ccggtttgcg actcccgctc
      601 gttcgaaata tccgcgagcg cgttccgcat tcttcgaatc accatcggta taaatgaccc
      661 gacccccctc ccccaccgcc tgtgcccacc aaatcgtcga gtatccgatc gccgacccga
      721 gttcaaatac tgtacgggcc ttaatcatca gcgccaactg ctgcaaaacg ttcgcaaccg
      781 cggggccgac tatcggtata ttgtgctcgg aggcatattc ttccatctcc gagagaacgg
      841 cctcgcgctt gggaagcatg gagtacaaat aatcatcgac cggtccagcc gtcgtgaagt
      901 tgtagtgccg ccaactcgga ggcgcatttt tcttcttgct tggagtcaca cgattccttt
      961 cgaacaaaac tctcttaacg cgagctatcc accgatcgtc tgtcccggct tcatctccac
     1021 cacttccact ccgggcacta acttctgcaa ctggtttggg cgtccggtca gcaccgtaaa
     1081 cgttccgaag tgcatgggga tgacggcctt cggcttgagc agattgcagg cataggcggc
     1141 ctcacgcggt cccatagtgt agtggtcgcc aatcggcagc atgaccacat ccggagcata
     1201 cagatcacgg atgatggcca tgtcaccaaa tacgttcgta tcgcccgcgt gataaatctt
     1261 ctgaccggag gaaaactcga tgacgtatcc gcaggcctcg ccgccgtaga tggtctcgtc
     1321 accgtcctga attccgcatg agtgatccgc atgaaccatg gtgactttga tgtctccaac
     1381 ggtttgcgtt ccacccttat tcatctcagc gatctgtttc gcacccttct tccccagcca
     1441 gcgagctagt tccggaatgc cgacgacaat aggattgtgc tttttgcaga tttccaccgc
     1501 atcaccgatg tgatcgccat gaccatgcgt gcagaaaagg atatcgactc tcttcacgtt
     1561 cttttcggaa tccgggcaca tgggattgcc catgatccat ggatcgata
//
LOCUS       DATIPF010000077         2556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1338970, whole genome shotgun sequence.
ACCESSION   DATIPF010000077 DATIPF010000000
VERSION     DATIPF010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338970"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VLL06_01960"
     CDS             complement(<1..734)
                     /locus_tag="VLL06_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HSS29761.1"
                     /translation="MMTLHSIGVLLVCVLAGVVLVWVAPAQADVEYFPIPSVSTSKND
                     GNDAGLIVPILTTDPDGELKYLVAPMLIRNSIVGTRGAINLFRYEPGGREMRFIGSFT
                     EQIERKLVFSYADPAFSQGRYSLNFGASFFKNATSRFFGIGQGTTESDQTNYTAREAR
                     MNWRFGIYANEVTQIAIGQRFRDVRLQHGATGLPFTGDQFPTVHGVQGESIILGHRAT
                     FYYDTRNSLVSPTDGMAVTAYAEVNQ"
     gene            complement(758..1417)
                     /locus_tag="VLL06_01965"
     CDS             complement(758..1417)
                     /locus_tag="VLL06_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSS29762.1"
                     /translation="MMCGVEGKRGVCRRMSIGIVLALFLCAGPVAQTVWAGGATDAMK
                     GSIDELLRVVQDKDLKQPGKTPERRMLLEQTVSNRFDFEEMSLRALGAPWKQLSDEEK
                     KEFVALFRTLLINTYREQIETYSGDGVQYLNERVEKAGDKEYAEVRTKIFLSDKSELR
                     LDYKLINKDKTNWRVYDVVIQDIGLISNYQKQFTKIIRASSYQGLVDQLRAKSDKIKA
                     P"
     gene            complement(1474..1917)
                     /gene="mlaD"
                     /locus_tag="VLL06_01970"
     CDS             complement(1474..1917)
                     /gene="mlaD"
                     /locus_tag="VLL06_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HSS29763.1"
                     /translation="MEKTKLELVVGAFVLVGILCLAYLSIKLGKLEMVGGDVYEVEAQ
                     FNSASGLKPGTAVEIAGVEVGRIRGITLIEDRAKVILAVNSTVKLYTDTIASIKSRGI
                     IGEKFLSLSPGGGGDPLKPGDTIRDTESGLDLEQLVGQFVHGKVN"
     gene            complement(1934..>2556)
                     /locus_tag="VLL06_01975"
     CDS             complement(1934..>2556)
                     /locus_tag="VLL06_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSS29764.1"
                     /translation="HMIGLLQPDRGEVWVGDVEISRLSGTRLNDVRKQFAMLFQGAAL
                     FDSLSVFENVAFPLREKLRMKGPEVGRRVEEKLEQVGLAGMGHKFPAELSGGMRKRAG
                     LARALVMEPEIILFDEPTTGLDPLMAKSIHDLIVTMHRRFGFTAVMVSHEIPEIFGIS
                     DWVAMLQKGRIALMATASEFQQTTNPEIHEFISVGGTEALPGSRVK"
BASE COUNT          545 a          782 c          644 g          585 t
ORIGIN      
        1 ttctggttca cttctgcata cgccgtgaca gccatcccat cggtcggcga gacaagactg
       61 ttgcgcgtgt cgtaatagaa cgtcgcccga tgtccgagaa tgatcgattc accttgtacc
      121 ccatgcaccg tggggaactg gtcgccggta aaggggagac cggtggcgcc atgttgcaga
      181 cgcacgtcgc gaaaccgctg gccaatggca atctgggtga cttcgtttgc gtaaatcccg
      241 aatcgccaat tcatccgggc ttcacgcgcg gtgtaattcg tttggtccga ctccgtcgtt
      301 ccttggccga taccgaagaa ccgagacgtg gcatttttaa aaaacgaggc gccaaagttc
      361 aacgagtagc gaccctgact gaaggccggg tcggcataac taaataccag tttgcgctca
      421 atctgttccg tgaaggatcc gatgaatcgc atttcacgtc cccctggctc gtatcgaaac
      481 agattgatcg caccgcgtgt tcccacgatg gaattgcgaa tgagcatcgg ggcaacgaga
      541 tacttgagct ctccgtcagg gtcggtggtc agaatcggga caatcaaccc ggcatcgttc
      601 ccatcattct tgctggtgga cacggatgga atcgggaagt actccacatc ggcttgcgcg
      661 ggcgccaccc acacgaggac aactcctgcg agcacacaga caagaagaac accgatgctg
      721 tgcaaggtca tcataatacg cactggactc aacgatgtta cggggctttg atcttgtcgg
      781 atttcgctcg aagctgatcg acaaggcctt ggtaggatga ggcacggata attttggtga
      841 actgtttttg gtagttgctg attaaaccga tgtcttggat gaccacgtcg tagacgcgcc
      901 aattcgtttt atctttgttg atgagtttgt agtcgagccg cagttccgac ttgtcggaca
      961 ggaaaatctt ggtcctcacc tctgcatatt ccttgtctcc tgccttttcg acccgctcat
     1021 tcaaatactg caccccgtcg ccggaatacg tttcgatctg ttcccgatac gtattaatca
     1081 agagcgttcg aaagagcgcc acaaactcct tcttttcttc atcggatagc tgcttccacg
     1141 gggcgccgag tgcgcggagg gacatctcct caaaatcgaa ccgattgctg acggtctgct
     1201 ccagcaacat ccggcgctca ggggttttcc caggctgttt caaatcctta tcttgcacga
     1261 ctctgagcag ttcatcgatg ctgcccttca tcgcatcggt cgctcccccg gcccatacgg
     1321 tctgcgcgac cggcccggca cagaggaaca atgccaggac gatcccgatg gacattcgtc
     1381 ggcagacgcc ccgttttcct tcaacgccac acatcatgat gcgtttctcc ttcttggttc
     1441 tcctccacag tcgtgaagtg tccggcacga tccctaattc accttcccgt ggacaaactg
     1501 gcccactaat tgttcgaggt cgagaccgga ttcggtatcc cgtatggtgt ctccgggctt
     1561 gagtggatcg ccaccgccac cgggcgagag cgagagaaac ttttctccga tgattcctct
     1621 cgatttgatc gacgcgatcg tgtccgtata gagcttgacg gtactgttca ctgccaagat
     1681 cacctttgcc cgatcctcga tgagcgtaat gcctcggatc ctgccgactt cgacgccggc
     1741 aatttcgacc gccgtaccag gtttgagccc cgaggcgctg ttgaattgtg cctcgacttc
     1801 gtagacgtca cctcccacca tctccagttt gccgagcttg atcgagagat atgccaggca
     1861 caagatgccg acaagcacga acgcgccgac caccagctcc agcttggttt tttccattct
     1921 cgatgctcct ctctcactta acacgcgatc cgggcaacgc ctcagtccca ccgactgaaa
     1981 tgaattcatg aatttcgggg ttggtcgtct gctggaactc agaggctgtg gccatcaagg
     2041 caatccggcc tttctgaagc atcgccaccc aatcggaaat tccgaagatc tccggaatct
     2101 catgactgac catgaccgcc gtaaagccga accgccggtg catcgtcacg atcaaatcgt
     2161 gaatggattt cgccatcaac ggatccaacc ccgtcgtcgg ctcatcgaag aggatgatct
     2221 ccggctccat gaccaatgcg cgtgccaatc cagcccgctt gcgcataccc ccgctcaatt
     2281 ctgcgggaaa cttgtgtccc atccccgcca accccacttg ctccagcttc tcctcgaccc
     2341 gtcgccccac ctccggaccc ttcatgcgaa gcttttcccg cagcgggaag gcgacattct
     2401 cgaacaccga gagcgagtcg aacagcgcgg caccctggaa gagcatcgcg aactgtttcc
     2461 gcacatcgtt caagcgcgtg cccgacagac gagagatctc cacatcgccg acccacactt
     2521 cgccgcggtc cggctgcagg agcccgatca tgtgct
//
LOCUS       DATIPF010000078         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1074311, whole genome shotgun sequence.
ACCESSION   DATIPF010000078 DATIPF010000000
VERSION     DATIPF010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074311"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..642)
                     /locus_tag="VLL06_01980"
     CDS             complement(199..642)
                     /locus_tag="VLL06_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSS29765.1"
                     /translation="MSAPLHRGLRHVALRVTNLARSRTFYEQLLGMKMVWEPDSDNVY
                     FSSGSDNFALHQIPPSELAVHQPMTRQLLDHIGVILESPEAVDRMYREVEPRLQQLGG
                     QVVKPPKQHRDGSYSFYFSDPDGIVIQALYEPAISKLEFTRKGEA"
     gene            1022..1828
                     /locus_tag="VLL06_01985"
     CDS             1022..1828
                     /locus_tag="VLL06_01985"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HSS29766.1"
                     /translation="MTVAAFESRMATEMSRLIVRYGGRPFVAPALREIPIQDNPVALR
                     FGARLIDRQIDILILMTGIGATTLFDILRSRHSLESIMAGLEQTAIVARGPKPVAALK
                     PLGITPTLTVPEPNTWVEVVSTLDEYRPVKGLRVAVQEYGISNPDLLEALKQRGAEVF
                     PIPIYRWALPEDTGPLKQVLGEIIAGNIQVMLVTNAAQIDHVMQILEREGTTAQFRDA
                     CRKMVVASIGPTASERLTHYDLSVDFEPSHPKMGVLVKETSEQAHALLSP"
BASE COUNT          473 a          614 c          548 g          457 t
ORIGIN      
        1 gccactccag cgacaaaggg gaagggccca ggagggtcgg cgctttctaa ttggacccac
       61 gtccagggcg cgaggctgag atactgcttt ttcggcagac tgtcccagat cttcatattc
      121 gtaaggcgtg aggcgcaagg cataagggga tcgcctcttc ctccttgacc tggtgacgct
      181 caaccatcgc ctcacccatc acgcctcacc cttacgggtg aattctagtt tgctaatggc
      241 gggttcgtac aacgcttgaa tgacgatccc atcgggatcg gaaaagtaaa aggaatagct
      301 gccgtcgcga tgctgcttcg gcggtttcac aacctgccca cccagttgtt gtagacgcgg
      361 ttcgacctct cgatacatcc gatcaaccgc ctcaggactc tcaaggatca caccaatgtg
      421 atccaacaat tgcctcgtca taggttggtg caccgccagc tcagagggcg gaatctgatg
      481 cagggcgaaa ttgtccgaac cggagctgaa atacacgttg tcggaatccg gctcccacac
      541 catcttcatt cccaacaact gttcatagaa cgtgcgcgaa cgcgcgagat tcgtgacgcg
      601 tagcgccaca tgacgtaatc ctcgatggag cggagcactc atagccatcc tcgatcgtgt
      661 ccgtatagta gggaggcatc caggctccgt caattgaaaa tcccgagcag gatgctgaaa
      721 aagtccgcca gcggcgttct cacttcgctc agaccctcaa cgtacaccag agggtacgcc
      781 tcggggcttc actcgctgcg gccttgctgg ctggcttttt gagcatcctg cctaatgcac
      841 tcctgctatc ccatacgcgc agagccatga gattccagcg tgccaagagt gtgtccgcct
      901 tgatcgggac gatggctgcg cctcctgttc cggcttatgg tatggtgagg atgctcacgc
      961 ttatgattta gattttagtt tttctttcgc gaccatgaca caaaacaagg gttttggagg
     1021 gatgactgta gccgcgttcg aaagccgtat ggcaacagag atgtcccggc tgatcgtacg
     1081 ttatggcggc cgacccttcg tggctcctgc ccttcgagaa attcccatcc aggacaaccc
     1141 cgttgcattg cgattcggcg ccagattaat cgacagacag atcgatatcc tcatcctcat
     1201 gacaggcatc ggcgcaacga ctctttttga cattctgcgt tcgcgccatt ctcttgagtc
     1261 aattatggca gggctcgagc agacggccat cgtggctcgc ggccctaagc cggtcgcggc
     1321 gctcaaaccc ctgggcatca caccgacact caccgtcccg gaaccgaaca catgggtcga
     1381 ggtggtctct acgctcgacg agtaccgtcc cgtcaagggt ctccgcgtcg ccgtgcagga
     1441 atacggcata tccaatcctg atttattgga agccttgaag cagcgcggag cagaagtgtt
     1501 tcccattccc atttatcggt gggcactgcc ggaagacaca ggccccttga agcaggtgct
     1561 cggcgagatc attgcaggga acattcaagt catgctggtc accaacgccg cacagatcga
     1621 tcatgtgatg cagatactcg aacgggaagg cacaaccgcg cagttcagag acgcgtgcag
     1681 gaagatggtt gtcgcgtcca tcggccccac tgccagcgaa cgtctcacac actacgactt
     1741 gtccgtggac tttgaaccct cgcacccaaa gatgggagta ctcgtcaaag aaaccagtga
     1801 acaggcccat gcactcttga gcccgtaacg cgcagaatac ggcacaatga atatgagaac
     1861 atggcgagta cgcacgcacg gcgtgctgtc gctctagcca ccgctcaccg gggggttact
     1921 cttcaattcg cgtgtagtat atcggcagct gaaaaagaga atccgataag ggccgtatct
     1981 tgacatgctg gtattccacg ctccaactgc cgtccttctt catcaacttc agcggaaaat
     2041 gtatatcggt cgccacccac tggtaataca cgtccgtccg ttcattccgt tt
//
LOCUS       DATIPF010000079         3150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_718482, whole genome shotgun sequence.
ACCESSION   DATIPF010000079 DATIPF010000000
VERSION     DATIPF010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718482"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(393..752)
                     /locus_tag="VLL06_01990"
     CDS             complement(393..752)
                     /locus_tag="VLL06_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29767.1"
                     /translation="MKKRIGIFCAIVLGVVFVTAGVCGEFAMANIGQEIRADFKDLKA
                     DLKELKRDLNAARHGELKGDVKNVKADLKELKGDIKGGTPGTPHSVPEPSPLILLGVG
                     LVGLAIIELWRRRKSAI"
     gene            928..1062
                     /locus_tag="VLL06_01995"
     CDS             928..1062
                     /locus_tag="VLL06_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29768.1"
                     /translation="MRRETVLLVRHALGAGLSSYVLVNNRAEGNAPLTVEGLVGMLRE
                     "
     gene            complement(1221..1508)
                     /locus_tag="VLL06_02000"
     CDS             complement(1221..1508)
                     /locus_tag="VLL06_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS29769.1"
                     /translation="MAKVLVIDDEPGIRDLLDTLLSRKGYDVVLADSGQKGLEVFRRA
                     RADVVVLDLKMPGMDGLTVLQQIRHLYPTQPVIILTGAGNPESEQRVRELG"
     gene            complement(1744..1935)
                     /locus_tag="VLL06_02005"
     CDS             complement(1744..1935)
                     /locus_tag="VLL06_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29770.1"
                     /translation="MHKAGDINHVHGLYRSGCCGIEHTLADNCKFPACPGPAHSGKNP
                     CAGKNAIWTLVRPTQMINR"
     gene            complement(2662..3054)
                     /locus_tag="VLL06_02010"
     CDS             complement(2662..3054)
                     /locus_tag="VLL06_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSS29771.1"
                     /translation="MLEQRIEEVNKANHAFYAAFGNLDIGEMDTIWAHQDYVTCIHPG
                     WTLRAGWPLVRDSWVLIFNNTFSMTFELTDVIVQVAGDIAWVVCVEHITTHQADEPQQ
                     AKVLATNLFERIGDEWLLIHHHGSAVMG"
BASE COUNT          777 a          831 c          824 g          718 t
ORIGIN      
        1 taatctgaaa tgtgcatgtc ctcgccgtcc gcctgtgact tttcgagaat tttaccctag
       61 gtaggctact acaggacgat cgaaggcggt ccatgtggat tgaaagcgat tcagtgttga
      121 atcaaatttg atttacctga acgaatcgaa ccctgaccca taacccgaat agggaggcca
      181 ctacagtttg acgaataggc ccgcgttgga tgtaacggtg cattgcacgg ggaagcgacg
      241 gtagggacga atcgagaacg gcatggacgc ctcgcggaca tggaagggta ttgtgtgctg
      301 gtgaacaacc gcggtgagag gagaacaaac ggccagtctc attgcggaga ccagcctccc
      361 tgtttgtcca ttcttcttca tgaagaggag aatcagatgg cgctcttcct ccgtctccag
      421 agttcaatga ttgctagccc tacgaggccg acaccaagaa ggatcagcgg tgatggctcg
      481 ggcacactat gcggagtgcc aggggtgcct cccttgatat cccctttgag ttccttaaga
      541 tcggctttga cattcttgac atcgcctttg agctcgccat ggcgagcggc attgagatcc
      601 cttttgagtt ccttgagatc ggctttaagg tccttgaaat cagcccttat ctcctggcca
      661 atattagcca ttgcaaactc accacagact ccggcagtca cgaacacaac tcccagcacg
      721 atagcacaga aaatgccaat tcgtttcttc atgtggactc ctttctttaa gggttgaaag
      781 aggaacaaat ccctcaacca ctgcgcctga agtctgacag aaatgtggcg ggtagaacct
      841 ggtaaaagcc ctagtttggt taaaaactct agaaggtgcg acaagacggg ccgaccgcag
      901 aacatgatca tcgaggagct accgcagatg agacgcgaaa cggtcctgtt agtacggcat
      961 gcgcttggcg cgggtcttag ctcctatgta ttggtgaata atagagcgga aggcaacgcg
     1021 ccgttgacgg tggagggatt agtgggaatg ttacgggaat agcacctctc gccaatcggc
     1081 tttctaggct ctgctctggg ggagaatcag gctcggcacg tgagacgacg tgtgcgacgt
     1141 cacctggtct ggcgtcttga ggaggcgctt cagcgcatcc cccaacagat gcagcgagaa
     1201 ctgtttttcc acgtactcgg tcacccgagc tctcggaccc gctgctctga ctcagggttt
     1261 ccggcccctg tgagaatgat aaccggttgc gtgggataga gatggcggat ctgttgcaag
     1321 accgtgaggc catccatccc cggcattttc agatccagga ccacgacatc agcgcgagct
     1381 cggcgaaaga cctccaggcc cttctgacca ctgtcagcga gaaccacatc atagcccttc
     1441 ctactgagaa gcgtgtcgag gaggtcccgt atacccggct catcatcaat cacgagcact
     1501 ttggccatga tccctcccca atagttatta atgggcaata atggttacta tggtaaccgt
     1561 atcatatgga gacaaatgtg tggagttggc cagatagcca gatcggaaat acataagaca
     1621 cgtacgcgcc aggccgacag aaaactagga ctgagcgtag ggtagagaga aaaggatgga
     1681 ctcaggagac tctttagaag tactgggtgg tggattttct aatgacgccg gttgaaacgt
     1741 gatttaccgg ttaatcatct gcgtcggtcg caccagggtc cagatggcgt tcttgcccgc
     1801 gcagggattc ttgcctgaat gggcaggacc tgggcaggcg gggaatttgc agttgtcggc
     1861 tagcgtgtgc tcaatgccgc agcatccaga ccgatacagg ccatggacat gattaatatc
     1921 gccggctttg tgcatgacgg cctcctacgc taacactgta acagaggaca gagccagcga
     1981 acccacggcc ttcccgaatg acactggagg gttgtccatg tctcactcaa taatgcgagg
     2041 tgacatacag cgcggccaca accataagga gaagaagacc ataactaata atgcgaataa
     2101 caatgcttcg ccatcgttcg ttcactcttc gagtttccgt ttacacatac aaacaacgcg
     2161 catgacttat ttcccttcgg caggaggcct ttttggcttc gttcgtttgt accagatgaa
     2221 aagaaacacg gcccacataa tccagaagcc catgatgctt gcaacgaacc acgggttgtc
     2281 agcaggtatg tcggttttaa gccagtccat gactattcaa cctataagag tgacaggcgc
     2341 acattgtaga accaggtcgg cttggctcag ctcttctcct tcggcagcag tcccgtcttc
     2401 tctagctcct gaacatgctc ttcgcgcata tccttgatgt agtcgcccaa ggtcttctga
     2461 tgtagcgcaa cggtcggcta gggtcggagc tacttgattc ctttccggca caatgctctt
     2521 acgaccttaa tctctgcctt tgaattccga cgggcgatcg gcgctatgtc gatatgtatg
     2581 ttagacacgc ccgcggtgaa aacttggcac caaccattgg caccagaggc gatcaaatat
     2641 cagaaaccat tgatttcagg cctaccccat caccgccgac ccatgatgat gaatcaacag
     2701 ccactcatcc ccgatccgct cgaagagatt tgtggctaat accttcgcct gctgcggctc
     2761 gtcagcttga tgggtggtaa tgtgctccac acagacgacc caggcaatat cgcctgccac
     2821 ttgcacgatc acatcggtaa gctcaaacgt catagaaaac gtattgttga agatcaggac
     2881 ccaggagtcg cgcacgagcg gccagcccgc ccgcaacgtc cagcctggat ggatgcaggt
     2941 cacataatcc tggtgcgccc aaatcgtatc catttcgccg atatcgagat tgccaaatgc
     3001 ggcatagaac gcatggttcg ctttgttcac ttcttcgatg cgttgttcca gcatgaatga
     3061 ttccttacct gaacaatgag ccgtatcata ctgcaatcac ggagacgagg gcgaggggag
     3121 aatggcaaaa cggttaagcc atcctcctac
//
LOCUS       DATIPF010000080         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1164195, whole genome shotgun sequence.
ACCESSION   DATIPF010000080 DATIPF010000000
VERSION     DATIPF010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164195"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            421..978
                     /locus_tag="VLL06_02015"
     CDS             421..978
                     /locus_tag="VLL06_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HSS29772.1"
                     /translation="MANHTKSGLLCSRLLRTACVLVLPCVLIAAAPTPTRPDYDGAHP
                     QYSKQEIHRAINWYAKKYRLDPALLRAVIKTESDFRQHAVSHKGAVGLMQLTPATSAR
                     LRVNDAYDSIQNIRGGAKQLRHLLTLYQGDLPLALAAYNAGVHRIKGGKVPRIRETRS
                     YVKKVLRYYEMFGSHPKPSSKHEKK"
     gene            complement(1056..1424)
                     /locus_tag="VLL06_02020"
     CDS             complement(1056..1424)
                     /locus_tag="VLL06_02020"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS29773.1"
                     /translation="MATQDPRSGRASSPNHEAAMAERRGRRLKLNCRLFFFGADDFEG
                     EATILDLSTGGCQATSLSEVRVGTTLRLSLFLQDQEWPLRIDEALVRWVKGPTFGLEF
                     TKIRPAQRERVRAIIMKAKL"
     gene            1707..>2544
                     /locus_tag="VLL06_02025"
     CDS             1707..>2544
                     /locus_tag="VLL06_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HSS29774.1"
                     /translation="MPRQINQRMAILAHSEIRAMTQACVQAKGLNMAQGVCDTPVPPV
                     VLRAAAQAMECGKNVYSRFDGLPELRQSIAKKLGQYNGIMADPETDITVSAGATGSFH
                     CACLALLNPGDEVILFEPYYQYHISALLAVEAVPVMVRMQAPDWTIHAVDVERAVTPR
                     TRAIIVNSPGNPSGKVFSRQELESVASIAQQHDLFVLTDEIYEYFLYDGRTHVSIATL
                     PGMADRTVTIGGYSKTFSITGWRIGYSVAATRWAQAIGAMNDLLYVCAPTPLQAGVAV
                     GIQ"
BASE COUNT          555 a          716 c          700 g          573 t
ORIGIN      
        1 ttcctgctat cccaaacgcg cgacgggtgt gtctagtgcg acgggcgcgg cgcgcaagcc
       61 atacttcaga gtgtctcacc tttcccgtca atctcgccta tctcgctccg ctgtcccaga
      121 tatgtagcca acgatgtttc gctgtgccaa aagggttctt acttaacctc cacacaatcc
      181 cgttcaacgg ttctgattct ctcacaaaaa tttccgagca gacttcgcct tcattcctgg
      241 taaagccgac agttctcaaa cgtccactgt gtgcacatca cgccacagta cgtcatgcgg
      301 catagtggtt gttttcccta tgccatccgc acgttagcag agactcctct cggcacggat
      361 cttgagacct ttgaggcctc tatgaaggtt gtcttgtgca cggggatgta gtcatacacc
      421 atggccaacc acacaaagag cggactgctc tgttcacgcc tgctgcgcac ggcttgtgtg
      481 ctggttctgc catgtgtgtt gattgccgct gctcctacgc caacgaggcc agactacgat
      541 ggtgcccacc ctcagtattc caagcaggaa atccatcggg ccattaactg gtacgccaag
      601 aaatatcgtc ttgatcctgc gttgctgcgc gctgtcatca agaccgaatc cgattttcgt
      661 caacatgccg tctctcacaa aggggcggtc ggcttgatgc agctgacgcc ggctacctcg
      721 gccagattgc gcgtgaacga cgcttatgac tccatacaga atatccgtgg aggcgccaag
      781 cagctgcggc atctactcac tctctaccaa ggcgatcttc cattggcctt ggcggcctat
      841 aacgccgggg tccatcgaat caaagggggt aaagttccgc gcattcgaga gactcgttcc
      901 tacgtgaaaa aggtgttgcg gtactatgag atgttcggtt cccacccgaa gccttcttcg
      961 aaacatgaga agaagtgagt gaacgaggcg caattctttt atgcggtgga gagcagcaga
     1021 gaatcgcgtg agcggagggt tgggcgagcg gattcttaga gcttggcctt cataataatg
     1081 gctcgtactc gttctcgttg ggcgggccta atctttgtaa actcgagacc gaacgttggg
     1141 cccttgaccc accgcaccag ggcttcgtcg atgcggagag gccattcctg gtcctgaagg
     1201 aacagggaca ggcggagcgt cgtgccgacc cgtacctcgc tcagggacgt cgcctgacat
     1261 cctcctgtgg agagatcaag aatcgtcgcc tcgccttcga agtcatctgc tccaaaaaag
     1321 aatagacgac aattcagctt gagacggcga ccccgacgtt ccgccatcgc ggcttcgtgg
     1381 ttagggcttg aagctcggcc ggatctggga tcctgcgtgg ccatggatcc tgtccttcct
     1441 cttacccgga gaacatctct atcgacccct acgactatac cgaacgaatt gtccgcccgc
     1501 ttcccccaag ggttctaatt cggaagaggg gccaacagca tcctgatcgc agtatagcga
     1561 atcggttgat gtctgtaacg aaaacttcgt aatccgctat tgtaatccta accgctctct
     1621 tcttcggtct gtgtagaatg agggcttgat atatctggaa cggctctgcg aaaagaaagg
     1681 ctctgtgaac aaggaggggt acgacgatgc cacggcagat taaccaacga atggccatat
     1741 tggcccattc tgagatccga gcgatgaccc aggcctgtgt ccaagcgaag ggcctcaaca
     1801 tggcacaagg ggtctgcgat acaccggttc cgccagtggt acttcgagcc gcggcgcaag
     1861 cgatggagtg cgggaagaat gtctactcgc gattcgacgg gttgccagaa ttgcgtcagt
     1921 cgatcgccaa gaagctaggg cagtacaacg gcatcatggc tgatccggaa acggacatta
     1981 ccgtcagcgc gggtgccacc gggtcttttc attgtgcctg cctggcactg ctcaatccag
     2041 gcgatgaagt catactcttc gagccctact accagtatca catcagtgcc ctgctggcag
     2101 ttgaagcggt tccggtgatg gtgaggatgc aggctcccga ttggaccatt catgccgtcg
     2161 acgtcgagcg cgcagtgaca cccagaactc gggcgattat cgtgaattcg ccgggcaatc
     2221 cctccgggaa agtgttcagc cggcaagaat tggagagtgt ggcatcgatc gcgcagcaac
     2281 acgatctgtt cgtcctcacc gatgaaatct acgagtactt tctctatgac ggtcgcacac
     2341 atgtgagcat tgcgaccctg cctggcatgg ctgaccggac ggttaccatc gggggatact
     2401 caaaaacttt tagtatcacc ggctggcgca ttgggtacag tgtcgcagca acacggtggg
     2461 cacaggcgat cggcgcgatg aatgacctgc tctatgtctg tgccccgacg cccttgcagg
     2521 cgggggtggc agtgggcatc cagg
//
LOCUS       DATIPF010000081         3046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1429226, whole genome shotgun sequence.
ACCESSION   DATIPF010000081 DATIPF010000000
VERSION     DATIPF010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429226"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..425)
                     /locus_tag="VLL06_02030"
     CDS             complement(138..425)
                     /locus_tag="VLL06_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29775.1"
                     /translation="MHRLIVVAFAALCLIGTSSFALADDAMKSEMGQMKADKDGMKAD
                     VKGKKVEMKAKTKAPKEAKKAKKEDMKGKAKAHKTKMKAKGAGQDMKTAGQ"
     gene            complement(568..1377)
                     /locus_tag="VLL06_02035"
     CDS             complement(568..1377)
                     /locus_tag="VLL06_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29776.1"
                     /translation="MPHIDSSIVLDSLKSLLLLLTLLITRMLIVRSIARNSAMSMEAK
                     RRWVVMTRNLILFALLIGLVVIWAHELQALTVSLVALAAALVLATKELILCLSGAALR
                     VGGKVYSVGDRIQIAGHRGVVLDHDIFATKLLEIGPGQAAHLYTGRVTVFPNSLLFTN
                     ALIKENPGQAYGLYILVIPLKDTDDWRVAERHLLEAARAECTPYMEEAGRQMKLLEQT
                     NLLEAPSPEPRITIQLSEPGRIQLVLRFPAPDRGRSRIEQAILRRFLDGPH"
     gene            complement(1407..1814)
                     /locus_tag="VLL06_02040"
     CDS             complement(1407..1814)
                     /locus_tag="VLL06_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29777.1"
                     /translation="MQGLLIAIVAGSVLACGGPWRDRYFDKGVDRLTQEEISERMGPP
                     HTAKTPALGGDTIWTFRVPVSDNELDPINLSSLTDATQQAAALLGKATNDAPKPTLYC
                     YRYTLSFNEQKILKQWKREECVQGTRNLLKANQ"
     gene            complement(1844..2038)
                     /locus_tag="VLL06_02045"
     CDS             complement(1844..2038)
                     /locus_tag="VLL06_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29778.1"
                     /translation="MIARFVGIVAIALGFVIGTYGLEDTGGLWMQTALGLLVTGMLAQ
                     GYAFYCSIKHRQQIHNRPKL"
     gene            complement(2160..>3046)
                     /locus_tag="VLL06_02050"
     CDS             complement(2160..>3046)
                     /locus_tag="VLL06_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSS29779.1"
                     /translation="PDSLIVLGGGSVALELGQFFARLGTRVTILQRGRTLLSEMDEAV
                     GRSLEAALIDEGIEVVTGVQLLRVTCDERKKTVWAMKDGRERSYAGEDILQALGRRPN
                     IEGLHLDLAKVTVDSGRIVVDGAMRTSQPHIYAVGDVNDVTPIVHLAIQQGEIAAYNV
                     THPDRPAKELDHRLDAEVVFTDPQVAVVGLNDAACHAGKIPFLTASYPFADHGKALCL
                     GASHGFVKLFCAPTTGELLGAQIVGPEAGELIHELIAVMYYHGTAADLLRMPHYHPTL
                     AEIVTYPAESIVEQLGMR"
BASE COUNT          667 a          926 c          777 g          676 t
ORIGIN      
        1 gtgagccgcc acggagaaac acgcggtgaa aacagtacgg cccgtcgtgg aacgttctac
       61 gacgggccgt gcatacgcct tctaaagcct gaggcgcctc gagcttactt gcccgccacc
      121 tcgacggcgt ctttcaccta ttgaccggct gttttcatat cttggcctgc acccttcgcc
      181 ttcattttgg tcttatgcgc tttggccttg cccttcatgt cttccttctt cgccttcttc
      241 gcctctttag gggcctttgt cttggccttc atctctactt tcttcccctt cacgtcggcc
      301 ttcatgccgt ccttgtccgc tttcatctgg cccatctcac tcttcatggc atcgtccgcc
      361 agggcgaacg agctggttcc aatcaggcac aatgccgcaa acgcgaccac gatcaatcta
      421 tgcacgagac acctcctcat caacggacgt taagttcagg accacacctc aattgctagg
      481 tgaagccgat tgaagcgtcg cacggcatcc ctggtctgtc aagccgatgc gcaaattcgg
      541 catggctcgt tcatgcacca acagacatca atgaggaccg tccagaaatc ttcgcagaat
      601 cgcctgctcg atcctcgatc ttcctcggtc tggtgctgga aaccgcagca ccaattgaat
      661 gcgtccaggc tcggacagtt ggatcgtgat gcggggctcc ggagacggcg cttcgagcag
      721 attcgtctgc tccaacaact tcatctgacg accggcttct tccatatagg gagtacattc
      781 agccctggct gcctctagca ggtgccgttc agcaactcgc cagtcgtccg tgtccttgag
      841 cggaatcacc aggatataga ggccatacgc ctggcccgga ttctctttaa tcaaggcatt
      901 cgtaaaaagc aaactgttcg gaaacacggt tacgcggccc gtatagagat gcgccgcttg
      961 gcccggcccg atctcaagga gcttggtcgc aaagatgtca tgatccaaga ccacccctcg
     1021 atgccccgca atctggatac gatcgccgac agaatagact ttccctccca cccgtaatgc
     1081 tgccccactc aagcagagga tcagctcctt ggtcgccagc acaagcgcgg ccgccaaggc
     1141 gaccagagat acggtaaggg cctgcagctc gtgcgcccaa atcaccacca gccctatgag
     1201 taacgcgaac agaatcaaat ttctcgtcat caccacccag cgtcgcttgg cctccataga
     1261 catggccgag ttcctggcga tcgagcgcac aataagcata cgggtgataa ggagggtcaa
     1321 cagcagcagg agcgacttga gactgtcgag gacgatcgaa ctgtcgatgt gtggcaccca
     1381 ggcaggcatg gattacagag gcgagatcat tgattggcct taagcagatt gcgggttccc
     1441 tgaacacatt cttcccgctt ccactgtttg agaatcttct gctcgttgaa cgacagcgta
     1501 tagcgataac aatagagcgt aggcttgggt gcgtcgtttg tggccttgcc cagcaaagcc
     1561 gccgcctgtt gcgtggcatc ggtgaggcta gagagattta tcggatccaa ttcgttgtca
     1621 ctgaccggaa ctcgaaaggt ccagatcgta tccccgccga gcgcgggagt cttggccgta
     1681 tggggcggtc ccatgcgctc ggatatttct tcctgtgtca atcgatcaac gcccttgtcg
     1741 aaatagcggt cacgccaagg accgccgcag gcgagtaccg atccggcgac gatcgctatt
     1801 agcagccctt gcatggcttt gcgagaaatc caactggaag acatcatagc ttgggcctat
     1861 tgtgtatttg ctgcctgtgc ttgattgaac aatagaaggc atacccttga gccagcatcc
     1921 ctgtcaccaa taaccctaag gccgtctgca tccacagtcc ccctgtgtct tccaatccat
     1981 acgtcccaat aacaaacccg agtgcaatcg ccacgatccc cacaaatcgc gcgatcattc
     2041 cgttcatagg tatcttgata ggtggcggct cgttatccat ggccatagtg ggtacgctac
     2101 actggatggt gcagccggtt caactccact tgccgtaggg tctccgtcat cgagtctctt
     2161 catcgcatac ccaactgttc gacaatggat tccgccggat aggtcacgat ctccgcaagc
     2221 gtaggatgat aatgcggcat gcgcaagaga tcggccgcag ttccatgata atacatcacg
     2281 gcgatcaatt cgtggatcag ttctcccgcc tcagggccga cgatctgcgc gccaagcagt
     2341 tcgcctgtcg tcggtgcaca gaagagtttc acaaaaccgt gcgatgcgcc gagacagaga
     2401 gccttcccat ggtctgcaaa gggatatgag gcggtcaaaa atggaatctt tcctgcgtga
     2461 caggcagcat cgttgaggcc cacaacggcg acttggggat ccgtaaagac cacctcagcg
     2521 tcgagacgat gatcgagttc tttcgccggc ctgtcaggat gcgtcacgtt ataggccgca
     2581 atctctccct gttggatagc cagatgcacg atgggcgtga catcgttcac gtcaccgacg
     2641 gcataaatat gtggttgcga cgtgcgcatg gcgccatcga ccacaatacg cccgctgtcg
     2701 acggtcacct tcgccagatc aaggtgaagc ccttcgatgt tgggccgtcg gcctaaggcc
     2761 tgaagaatat cctcgccggc gtatgaccgt tcacgtccgt ccttcatcgc ccagaccgtc
     2821 ttctttcttt cgtcgcaggt cacacggagc aattgcacgc ctgtgacgac ttcgatgccc
     2881 tcgtcgatca atgcggcttc cagcgagcgc ccgacagcct cgtccatctc agaaaggagc
     2941 gtccgtcccc gttgaagaat tgtgaccctc gtcccaagtc gagcaaaaaa ctgaccgagc
     3001 tcgagagcaa cagacccacc acccagcaca atcaacgagt ccggta
//
LOCUS       DATIPF010000082         4751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_810663, whole genome shotgun sequence.
ACCESSION   DATIPF010000082 DATIPF010000000
VERSION     DATIPF010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810663"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(477..638)
                     /locus_tag="VLL06_02055"
     CDS             complement(477..638)
                     /locus_tag="VLL06_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29780.1"
                     /translation="MTDNNYTAFMFLKALPKVIGVCVGALVSIYFVAYIWVYGFIPDL
                     ISSIVHRIK"
     gene            complement(711..1088)
                     /locus_tag="VLL06_02060"
     CDS             complement(711..1088)
                     /locus_tag="VLL06_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HSS29781.1"
                     /translation="MTKEQAVKNGHVDVTTLERVLQWHGAFRRSLGPIRVTPLQAAML
                     LFVSRHADAKLKDAANALCVRPPTLNDVVKDLVRKRWVTKRRSVVDTRVVHLHLSRRG
                     EALTHQIGQRVHQVTITLTEEVR"
     gene            complement(1393..1566)
                     /locus_tag="VLL06_02065"
     CDS             complement(1393..1566)
                     /locus_tag="VLL06_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29782.1"
                     /translation="MVVGRLWTSYGASAPDVVVPDGITVLQQVRSLNPDQPVIMLTGH
                     ERQRRNTWYTRYA"
     gene            2137..2367
                     /locus_tag="VLL06_02070"
     CDS             2137..2367
                     /locus_tag="VLL06_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29783.1"
                     /translation="MTKAQAALLQRKWKKQGDPPCEHLIQELSDVSSDFDDGHVLNAH
                     HCRECGELFVHRYKGKGLSNNPSTEPEFIGET"
     gene            3293..3607
                     /locus_tag="VLL06_02075"
     CDS             3293..3607
                     /locus_tag="VLL06_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29784.1"
                     /translation="MRNAVQVSCLLGFIVLSGCANLTTPSDSLPSLPCPVTSTNAPPS
                     VSQNQTIMTQALQKRLQEKERTIATQTQQIEVLSSQLEALKQIEQDTHRQPRKRLTYP
                     IP"
     gene            4079..4489
                     /locus_tag="VLL06_02080"
     CDS             4079..4489
                     /locus_tag="VLL06_02080"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HSS29785.1"
                     /translation="MWAAKERNPIEDFTLLGKGADFKGVVTFDGTVRVDGRVEGEIHT
                     TGTLIVGEHAVIEGIICAGVLMNSGKIHGTITAIEKIHILKPGLLVGDIRTPVIAIEE
                     GSQFHGMCDMGAHHWSNERTLSVDVSPGLLASAH"
BASE COUNT         1105 a         1304 c         1213 g         1129 t
ORIGIN      
        1 ggcatctacc accgcgacga gtaccagccg atgaccgcag aggggccttg ctttcaaggc
       61 ccctttttct ctgctgtgtg gtcggtgtcc ggcgtattcc cttcccgacc tcatcgagga
      121 cgtcacttga gtaggccgct cgcccttcat gattagaagt ggtgtcgagg gacagacggc
      181 acgacaatcg ggctgcttgc agggatgttg agaggctgaa tcgatggggt ggatgctgag
      241 cctactcgtt agacgtcttt gcctctaacc tctacatatt ttcgactgca tccgcagtat
      301 tcgttgccgc gttagctgac aatccaattc caccagattt tcctccgatc gtgcacaatc
      361 gtcagctgag gcattcatta ggttaatcat gggggcattc tttgcgctag tgctggcaat
      421 cggaatcgct accagcacac gaaagtaatg tgattgattg gagcggttcc tttcgatcac
      481 ttgatccgat gtacgataga cgagatcagg tcgggaataa atccatagac ccaaatgtag
      541 gcgacgaaat agatagagac gagcgctcca acacacacgc cgatcacctt cggtagagcc
      601 ttcagaaaca tgaaagccgt ataattgtta tcagtcattc gcaccagcat tgcgggttaa
      661 gggactaata acctcagatt tgtgcggttc tgcggggtca cgccgaggga ttatcgtacc
      721 tcttcggtga gtgtgatagt cacttggtgt acccgctgcc cgatttgatg agtgagcgcc
      781 tcaccccgcc gactcaggtg caaatgcacg acacgcgtat ccacgaccga ccgacgtttg
      841 gtcacccacc gcttccgaac gaggtctttc acgacatcat tcaaggtcgg cggccttaca
      901 caaagcgcat tagccgcatc cttcagtttg gcatctgcgt gacgactcac aaaaagaagc
      961 atcgcagctt ggagcggagt cacacggatt ggcccgagac tccttcggaa cgcgccatgc
     1021 cattgcagca ctcgctctag ggtagtcaca tccacatggc cgttcttcac tgcttgctct
     1081 ttggtcatgg gtcacatgct ccttttcttc ccaggtaatt atggccgtct ttgtaacaac
     1141 atggcccgac accttcgctt cttctatgga cagcacagca tcagacctcc atttgaggtg
     1201 aggtgctccc actgatgtcc cgtattatgg acaggaatgc gccattggta agtaggcact
     1261 accctagaat tggagtaaga catttcctac gacacccgcc aagaagcgag tcggtaaatt
     1321 actcacgata tgtatgctgt cttgagaacg cccccggcag agtcagcgag aattccttcg
     1381 cgacgaacgc gctcacgcat agcgcgtgta ccaggtgttc cttctctggc gttcatgccc
     1441 cgtcaacatg attaccggtt gatcgggatt gagactgcgg acctgttgca agaccgtaat
     1501 cccatccggc accacgacat cgggggcgct cgcgccgtaa gaggtccaaa gccttccgac
     1561 caccatcggc gcggactacc tcatagcgtg catcgaggag atttcggatg ctctgctcat
     1621 cgtcaatcac gagaatcctg gctatgaaac acccagaata aaaaatggtg cggccttaat
     1681 gacaggagct gccaggcttg gcagcagttc ccgctccagc tagggtttca tccagcgtca
     1741 tttccattaa gccgaatggt agagtgggag ccatggatcc ctttccgcgg tcagcgatga
     1801 aggaattcct gcacatctgt cttcgcttgt caccatattt gaccgacgag gaatgtgaag
     1861 aactcgcatg cctgatgtat gtggtccgac aacggactgt cctcccctat gatgatttag
     1921 atgacgtagc cgcatcgttt gcgttcgcca aactttcttc tgttgactga cttgctcatt
     1981 gctcacctcc tcattaatct gccttcggat aatctcccag gcgatacggc tagagcgcaa
     2041 tacaggtcgt aagagcaccg gaggtgtggt cggcagatcc agcgctaggg agctttctag
     2101 tctcgatctc tgggagtgcg tagtacgatc cgtcgcatga ccaaggctca ggcggctctc
     2161 ctccagagga agtggaagaa gcaaggcgat ccaccctgtg aacatttgat ccaggaatta
     2221 tctgacgtgt cgtctgactt tgatgatggg catgtgctga acgcccatca ttgccgtgag
     2281 tgtggagagc tgtttgttca caggtataag gggaagggct tgtccaataa tccttcgacc
     2341 gaaccagagt tcatcggcga gacgtagaga attcgatcgg ctgaaaactc ttcaagaatg
     2401 gtgtcctttc ccctcgggat ttcactagaa tcgtcctcct gctcgcaacg ataccggtag
     2461 acgcatgaag tcaaccatcc gactcgaagt ccaagaactc atatctgtgt gcaaaaatct
     2521 tgcgagacaa gaggtggcgc tcacggtccg cgagtgccaa gcccttatct gctgcatccg
     2581 agagctagaa aagcttgacc gccatgatac cgtggacgca tggaatcgac ccttcggcta
     2641 ggggtccagg aactcatagg tgtctatgga tcctcgagag actcgatggt gtcctcaccg
     2701 tctcggagtg tgaagccata ctccgctgtc cgaacagctt gaaaagaaag tctttccaga
     2761 tcgtcagaga tctgaccgcc cgatactcta tcccacgtct caccgataga ctgtcccaca
     2821 gggccatgga gcagaaatga cgaaggggat aactcttctc atcgggctgg tcctctgcct
     2881 gctgccgctc tccgaatagg ctcagaatgt cgaggccccg ctcttaatcc gagttcgagc
     2941 accacgcaat caatccatcg agatcgatag agggttagac ctgatgaccg tccggggcat
     3001 cacaaatctc atagactgtg gagcaaagcg tcacttgttc tgcccagtat gtgcgctgtt
     3061 tggtgccccc atcgatcagc agtatcgagg tcttccgagg gaagtcggaa tctgtttgca
     3121 tccatgagag ttccaatttt cgaggttgca attacatcaa tacgaggtgc cgtcaagtgg
     3181 gtatacatgc cataagacct tccccgaacg cgggggcttc actttctgga acttggtggt
     3241 agctttaagc cttgctccct atggaatctg caaaaccgtt taagggtggg acatgcggaa
     3301 cgcggttcaa gtgagctgcc tgctaggctt catcgtgcta tccggctgtg cgaatctcac
     3361 aacacccagt gattctcttc cctcgctccc ctgtccggtg acctccacca acgctccgcc
     3421 ctccgtgagc cagaatcaga ccatcatgac ccaagcgtta cagaagcgtc ttcaggagaa
     3481 ggaacggacc atcgccacac aaacccaaca gattgaagtc ctctccagtc aattagaagc
     3541 cttgaagcag attgaacagg acacgcatcg ccaacctcga aaacgcctca cctatcccat
     3601 cccatagtaa cgtccccgat tgatggccct gagaaagaga attgtcaacg gtataccgtc
     3661 ttagagacga cggaaacaca tatttgaaga ttcatatttt ttggaatgtt ttcgtagttg
     3721 gatgtaatac tagggtcttt cctagtcctt ccatgtcgta tttctgtcaa tctggctcgc
     3781 atgacacagg ctcaagcctt tcaagccatg ccgccagtag atgcagccat cgttgacaca
     3841 ttgcaaagaa acgggccgtg tcgcctcaga gacatagcca cgtcccttcc gagttacagt
     3901 tgggtggagg tcctcgccgc tgtagatcgg atgtcgcgag acaagcgagt ggttctctcc
     3961 caactcgatc agacaaccta ccaactgtca cttggtcctt atgccacata gcacacgact
     4021 gaaggtgaca ccgcgcagtg ccacgcacat atgagaagaa tcttaggagg aggcaatcat
     4081 gtgggcagcg aaagaacgga atcccatcga agactttacc ctattgggga aaggggcgga
     4141 cttcaagggc gtcgtgacgt ttgacggaac cgttcgggtt gatgggcgcg tcgaaggcga
     4201 gattcataca acaggaacgt taattgtcgg agagcacgcg gtgatcgaag gtattatatg
     4261 cgccggggtg ctcatgaata gtggcaagat tcatggcacc atcacggcca ttgaaaagat
     4321 tcacatctta aagcccggtc ttctggtcgg cgatatccgg actcctgtca ttgcaatcga
     4381 ggaaggctca caattccatg gaatgtgtga catgggtgct catcactgga gcaacgaacg
     4441 caccctttcg gtcgatgtct ctcctgggct tcttgcctcc gcccattagg ctagaggggg
     4501 aaacccctct agctctttgc ttctggatgt cgcgaatgtc ctccgcaccg gccctgctgg
     4561 gaagctcctg acaccgctga gcaaaacgtc cacaactcgc ctgtaacctt ccgagtggtt
     4621 cctgttcaat gtttctctgt aactgccccc caatccctgc acggttaacg gcgaaggctt
     4681 ccttgagcgg aagcgcattt ttttccgcct ttcgcttgtt gaccagtata tacgctcgcc
     4741 gagccgtctc t
//
LOCUS       DATIPF010000083         3749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1429381, whole genome shotgun sequence.
ACCESSION   DATIPF010000083 DATIPF010000000
VERSION     DATIPF010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429381"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..518
                     /locus_tag="VLL06_02085"
     CDS             282..518
                     /locus_tag="VLL06_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29786.1"
                     /translation="MTTRFVGLMTAALACTFLVAACDSTKPPSADCIEAARLYAEALD
                     QNTRAPADEKAENMAARKNAITVLEERKVKACGD"
     gene            complement(648..803)
                     /locus_tag="VLL06_02090"
     CDS             complement(648..803)
                     /locus_tag="VLL06_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29787.1"
                     /translation="MGEYTAECVHLGTGTSRVNEAEAAEVKSVPVRVMNGAAVHMNIV
                     AGIGVLK"
     gene            complement(857..2293)
                     /locus_tag="VLL06_02095"
     CDS             complement(857..2293)
                     /locus_tag="VLL06_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29788.1"
                     /translation="MPEHDLEKLLGGFAADTLTQEEKQALYTAALQDQQLFNALADEQ
                     ALKELLSNPDVRRRLLTSLEQKKAPAVDSPLPWLDWFRRPAGLALAGGLTAAALAVVL
                     GVRIYQDSLKKTSQSVATEAATPASPPMPMPPASQPPAPQVREPQPKSKKDTGPAHEM
                     ERKDRRFDNLTTREQAAPPASKHEAASDVARDRLKQQPAQEEVRREANAPVAALGKSA
                     EEVPSSAEQKLAGSSRPPAKASVPAPMPATTGAPAAGVGTPAVNARALFYGANETRQD
                     QSRIAREKERTTKPLAESAPQANRLGRPLEGFSQPGKAVGTIGQLTPLGLRYSFVVRG
                     TDGQEREVDAATASKSTEPIFLTMEANQEAYLQVWKTAGPSTPQLYWPQKETGQVSLT
                     MIAGRRQQIALPMERGHVTFTAHLSRVPFGPTTEHEAAMFDRLSPNQIQESITASDQT
                     GSHEQATYVVSQDPSIPTQIVVEMTLVR"
     gene            complement(2354..2851)
                     /locus_tag="VLL06_02100"
     CDS             complement(2354..2851)
                     /locus_tag="VLL06_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSS29789.1"
                     /translation="MDRDQILISLRERILAFATLRVSRAQAEDLAQEVLVVLHEKYAH
                     VTELTELVPLAFQVLRFKMLDAHRKSLRRGEYNQESVDDLPLADPGDDPATQLDQKQR
                     VDRLLAVMAQLGGRCRELFKWKLEGKSFPEIQKIMSQTSINTIYTWDLRCRKQLLTLM
                     GGSWE"
     gene            complement(2867..>3749)
                     /locus_tag="VLL06_02105"
     CDS             complement(2867..>3749)
                     /locus_tag="VLL06_02105"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSS29790.1"
                     /translation="RSPLFRYLLWKHQLSPSNPREANKKYLDDLLIELRASRHVQRAV
                     LLGMDGVYDQNGQLNQAHTDFLISNDYVLKTARAHPSEFLAGVSINPQRRDAVEEVHR
                     CADAGAALVKVLPNAQQFNPADLHYKAFYRALAERKLPFLSHVGYEFSLIGKDQSVGD
                     PDRLRPALEEGATVIAAHACSYGLIIYEKFLPTLRDFVQRYPNFYADISALTLPNRVQ
                     MLLHLRRYPEIHERLLFGTDYPLSVFHLAAWGRVALGTLYDIVRTKNRFDRQVLVCRR
                     LGLGFRSLGDIVTPPKL"
BASE COUNT          721 a         1015 c         1118 g          895 t
ORIGIN      
        1 cattgttgga ttgtccctct taaggctcta tcggtgatgt cgggactgaa acgataaggt
       61 ttttcgttct cttaatgagg tttgcatcgc ggtcgttcca ctccactttg actcatggaa
      121 gtggaggccc gcccatctcc cataccaatc tgccctgtga atatcgttga acggtcttta
      181 ccccgactcg acaatcacac tggaatttgc gagtaagttt tgcgtaagat gccggtcggt
      241 atggccaaga gtcgggtgtc taacattgga ggatgtgcac aatgaccaca cgttttgttg
      301 gactgatgac tgctgcccta gcttgcacat tccttgtcgc ggcatgcgat tcgactaagc
      361 cgccgtcagc agattgtatc gaggccgcac gcttatatgc agaagccctg gaccaaaata
      421 ctcgcgctcc tgcagacgag aaggccgaaa atatggccgc tcggaaaaat gcaattaccg
      481 tattggaaga acggaaggtc aaagcctgtg gagattaagt ggagagctga gccccttgca
      541 gccgtaacct gcagcattct atagaacgat gtctttcagt cggcaacaca gggggcgtgt
      601 cgtgagacac gccccctgtg ttttgagccg tcagttgcca gccacggtta tttcaagacg
      661 ccaatgccgg cgacaatgtt catgtgaact gccgcgccat tcatcacccg caccgggact
      721 gacttcacct cggctgcttc cgcttcattc actcgcgacg tacccgtgcc gagatggacg
      781 cattccgcgg tatattcccc catacgtaga caagtaatag ggaacattct ggtgtatcga
      841 ggccatgtaa ttgcggctac cggacgaggg tcatctcgac gacgatctga gtgggtatgg
      901 atgggtcttg gctgacgacg taggtggcct gttcgtgtga gccggtttgg tcgcttgcgg
      961 tgatggactc ttggatttgg ttgggagaga gccggtcgaa catggcggct tcgtgctcag
     1021 tggttggtcc gaatgggact cgggaaagat gggccgtgaa ggtgacatgc ccgcgctcca
     1081 taggcagggc gatctgttgc cggcgaccag cgatcatcgt gagagagacc tgaccggttt
     1141 ccttctgagg ccagtacagt tgcggcgttg aggggccagc tgtcttccag acttggagat
     1201 aggcctcttg gttggcctcc attgtgagaa atatcggttc tgtgctcttc gatgctgttg
     1261 ccgcgtctac ttctcgctct tgcccgtcgg ttccacgaac gacaaagcta tagcggagtc
     1321 ctagaggcgt gagttggccg atcgtgccca cggctttgcc cggttgtgaa aacccttcta
     1381 atgggcgtcc aagccggttg gcttgcggtg ctgactcagc caggggcttc gtggtccgtt
     1441 ccttttctcg agcgatcctg ctttgatcct gccgcgtctc gtttgcgcca tagaacaggg
     1501 cacgcgcgtt gactgccggt gtgccgacac cggcagcagg agcgccggtg gtcgctggca
     1561 taggtgctgg aactgatgcc ttggctggtg gcctagagct tcctgcgagt ttctgctccg
     1621 ccgacgaagg cacttcttct gcggacttgc cgagggcggc cactggcgcg ttggcttctc
     1681 tgcggacttc ctcttgcgca ggctgctgtt tcaaacggtc ccgggcaaca tcggaagccg
     1741 cttcatgctt cgatgccgga ggcgcagcct gctctcgtgt ggtcaagttg tcgaaccgcc
     1801 tgtctttcct ttccatttca tgggccggtc ccgtgtcctt cttggatttc ggctgtggtt
     1861 cgcgaacttg gggtgccgga ggttgagagg ctggaggcat tggcatcggt ggagatgcgg
     1921 gcgttgccgc ctcggttgcg actgattgcg acgtcttttt gagactgtcc tggtatattc
     1981 tgacgcccaa caccaccgcg agggcagctg ccgtgaggcc accggccaag gcgagccctg
     2041 ccggacggcg gaaccaatcc aaccatggta gaggactgtc gacggctgga gccttcttct
     2101 gctccaatga cgtgaggagt cggcggcgta cgtccggatt agagagcaac tctttcaggg
     2161 cctgctcatc agccagcgcg ttgaacaatt gctggtcttg cagtgcagcg gtatagagcg
     2221 cctgtttctc ctcctgagtc agcgtatcgg ctgcaaagcc gcccagcagt ttttcgaggt
     2281 cgtgttcagg catcggactt tctcgtttgc ccctttccac ctaggagcag cagtctgcct
     2341 gttgctccca ccttcattcc catgatccgc ccatgagggt taatagctgt tttcggcagc
     2401 gtagatccca ggtatagatc gtattgatcg aggtctgact catgatcttt tgaatctcgg
     2461 gaaagctttt cccttccaat ttccacttga acagctcgcg gcagcgccct ccgagctgtg
     2521 ccatgactgc aagcaaccgg tcgacgcgct gcttctgatc cagttgggtt gctggatcgt
     2581 cgccgggatc ggcgagtggg aggtcatcaa ccgattcctg attatattcg cctcgtctga
     2641 gcgacttgcg atgagcgtcc agcattttga accgcaagac ttggaaggcc aggggaacca
     2701 gttcggtcag ctctgtgacg tgcgcatact tctcatggag aaccaccagc acctcttgag
     2761 ccagatcctc ggcttgtgcc ctcgataccc gtaatgtcgc gaaagcgaga atcctttctc
     2821 tgaggctgat tagtatctgg tctcgatcca tagtgccgcg tgaatgttac aacttgggcg
     2881 gggttacaat gtcgcccagc gatcgaaagc cgagtcccaa ccttcgacag accaacactt
     2941 gccgatcgaa acgattcttc gttcgaacga tgtcgtagag tgtgccaagc gcgacgcgcc
     3001 cccaggctgc cagatggaag acggacaggg gataatcggt cccgaagagc agccgttcat
     3061 gaatttcggg atagcggcgt aggtggagca gcatctggac ccgattgggc aatgtgaggg
     3121 cggagatgtc cgcataaaaa ttcgggtacc gttgtacgaa gtcgcgcaga gtcggcagaa
     3181 acttttcata gataatcaat ccgtaactgc aggcatgtgc ggcaatcacg gtagcgcctt
     3241 cctccagcgc cggcctgaga cgatccgggt ctccgacgga ctggtccttt ccaatgaggc
     3301 tgaactcata gccgacgtga ctcaggaacg gcagcttccg ttccgcgagc gctcggtaga
     3361 acgctttgta gtgcagatca gccgggttga actgctgcgc gttcgggagg accttcacca
     3421 atgcggcgcc ggcatcggcg caacgatgga cctcttcgac ggcgtcacgt cgctgaggat
     3481 tgatcgagac gccggcaagg aactcactcg gatgggccct cgcggtcttg agcacatagt
     3541 cgttgctgat gagaaagtct gtgtgcgcct ggttgagctg gccgttctgg tcgtacacgc
     3601 cgtccatacc aaggagtacg gcgcgctgaa catggcggga ggcacgcaat tcgatgagca
     3661 gatcgtcgag atatttcttg ttcgcttccc gcgggttcga tggagacaat tggtgtttcc
     3721 ataggagata gcgaaatagc gggcttcgc
//
LOCUS       DATIPF010000084         1758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_15110, whole genome shotgun sequence.
ACCESSION   DATIPF010000084 DATIPF010000000
VERSION     DATIPF010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15110"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VLL06_02110"
     CDS             complement(<1..436)
                     /locus_tag="VLL06_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HSS29791.1"
                     /translation="MINVSKLLKELILYTCTLPAFKPGLEWQEHLVQVILHQLQAVRM
                     VPLQLPHLADPRAARVAEILMTDPGDVRTLAQLSRIAGASKRSIERLFRQEIGMTFGK
                     WRQQLRLMQGMRLLAEGAKVTHAALESGYSTPSAFISMFRKAL"
     gene            822..1421
                     /locus_tag="VLL06_02115"
     CDS             822..1421
                     /locus_tag="VLL06_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSS29792.1"
                     /translation="MNLIHQWLCRSDRWRATLQQRVPWVLDGNDLGPNVLEVGPGPGL
                     TTDVLRMSVHRLTALEIDPKLASALTARLHGGNVEVFEGDATDMPFRDSEFSGVVSFT
                     MLHHVPSPELQDKVLLEVFRVLQPGGFFLGSDSRQSLLMRLIHIGDTFVPIDPNRFGT
                     RLETAGFEVHAVEKRSEAFLFRARRPFCDCGERLGGSFV"
     gene            1453..>1758
                     /locus_tag="VLL06_02120"
     CDS             1453..>1758
                     /locus_tag="VLL06_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29793.1"
                     /translation="MKQYMDRKPGSSPRSLCFPVVVLVAATLAIPALAATNSGECSTN
                     PANRQLDFWLGNWAVTYPGVTSGATSTVSLDLNKCMIVENWNGHSGHSGKNMLAYSAD
                     "
BASE COUNT          384 a          467 c          491 g          416 t
ORIGIN      
        1 ccagagcctt cctaaacatg gaaatgaacg cgctcggtgt actgtacccc gactccagag
       61 ctgcatgtgt gacctttgcg ccttccgcta acaggcgcat tccctgcata agcctgagtt
      121 gctgccgcca tttgccaaaa gtcattccga tttcctgtcg gaagagtctt tcgatggatc
      181 tcttgctggc cccggctatc cggctgagtt gagcgagggt tctgacatcg cccgggtccg
      241 tcataagaat ctctgcaacg cgggcggctc tcggatctgc caaatgaggc agttgtaatg
      301 ggaccatccg caccgcttgc aattgatgaa gaattacctg gaccaagtgt tcttgccatt
      361 ccagccctgg cttaaaggcg ggcagagtgc aagtgtagag aatgagttct ttcagcaact
      421 ttgacacgtt gatcacacag cagtctcgtg gcagtccctt tgcgattcgc ggtttcaaat
      481 acagagttcg catcgccact gcacctgaca tcgtgattgt gtgcgcgatc tgcgcaggta
      541 tccataccgc gcgattgggt gggaccaccc atgtaccatc gcgagtacga attgtcatca
      601 ccccatgcga cgcatagacc agttgatcac ggtcgtggaa gtgcagtggt attacgtaat
      661 cggctcgata gtctcgggtg agagtcgcaa tcgcagacga acgatcgggg gagcgatcga
      721 aaattccatt gtgccgcttt tgcgacattc attgtcacct tatcgaaagc gcgcagaact
      781 cgcaagggat acggtaagag ccacgaatag cccttgggga tatgaatctc atacatcaat
      841 ggctctgccg ttcggatcgc tggcgtgcaa ccctgcagca gcgagtgcca tgggttctgg
      901 acggaaatga cctcgggccg aatgttttgg aagtcggacc tgggccgggt ttaaccactg
      961 atgtgctgcg gatgtcggtt catcgactga cagctctgga gatcgatccg aagctggcga
     1021 gcgcgctgac cgcacgcctg cacggaggca atgtggaagt gttcgagggc gatgccaccg
     1081 acatgccgtt ccgggattca gagttctcgg gagttgtctc attcaccatg cttcatcatg
     1141 tcccgtctcc cgaactccaa gacaaagtcc ttcttgaggt gttccgcgtt ctacagccag
     1201 gaggtttctt tttgggcagt gacagtagac aaagcctgct catgcgactg attcacattg
     1261 gggacacatt cgttccaatt gatccaaaca ggtttggtac gcgacttgag accgccggat
     1321 tcgaagttca tgccgttgag aagcgtagcg aggcgtttct cttccgcgcg agacggccat
     1381 tctgcgattg tggagaaaga ttgggaggct ccttcgtttg aaagggagcg cacctcatta
     1441 aggagaatcg ttatgaagca gtacatggat agaaaacccg gatcgtcccc ccgttctctc
     1501 tgctttccag tcgtcgtgct tgttgcggcc acgctagcca ttcctgcctt ggctgccacg
     1561 aattcgggtg aatgttcaac caacccggct aatcggcaac ttgatttctg gctcggaaat
     1621 tgggccgtta cgtatccggg agtcacatcg ggtgccacga gcacggtcag cctggatctc
     1681 aacaagtgca tgatcgtcga gaactggaac ggacacagtg ggcacagcgg caagaacatg
     1741 ctcgcttata gcgctgac
//
LOCUS       DATIPF010000085        10473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_101863, whole genome shotgun sequence.
ACCESSION   DATIPF010000085 DATIPF010000000
VERSION     DATIPF010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 10473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10473
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101863"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2512)
                     /locus_tag="VLL06_02125"
     CDS             complement(<1..2512)
                     /locus_tag="VLL06_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSS29794.1"
                     /translation="MNKHSASPCETLAEQYRTLLEVAEAISSHRDLHELFRDLAQRLP
                     RVVHVNFVALSLHDPVRNTMRLHTIQANVPADLVGGHEGPVEETPDGLVWHTQQPILV
                     ANIIEEGRWPKVTQCMQEDGVNSFCVVPLTTAVRRLGAMGFLSLEKDAYGERDLEFLQ
                     QVAKQIAVAVDNVLHHQDLIHDRDRLRLLLEVSEAIVSHRDLSSLFRDLAQRLPAIAA
                     FDFIGLILHDPAKNLMKVHVLETAAAHHLTTKLDGLELPIEESSSGWVWTHQQPLIIP
                     SLAEETRFTKGMAALKDIGVQSVCLFPLTTAMRRLGAIGFGSLETRAFGEKNLGLLGQ
                     VAKQVAVAVDNVLNFAGTEAAQQSLARERDRLSLVLEINNAVVSHLELPKLLKAISGC
                     LGRVIPHDLAWFCLYDPATHQLQTHALDFPSHQDFTEAGDPIPLEGTPEGLAFTTQQT
                     VLIRSLDLSEFPAEIMKRGAAAGLKSGCAVPLLSHGRGLGTLSVVSKQESAFTEDDAK
                     LLSWIGAQVALATANSMAYQEISSLKDKLAKEKLYLEEEIQTEYNFEEIVGDSRALKL
                     LLKEIQTVAATDSTVLILGETGSGKELVARALHNRSDRRERTFVKLNCAAIPTGLLES
                     ELFGHERGAFTGAIAAKIGRFELADRGTIFLDEVGEIPLELQVKLLRVLQEQEFERLG
                     STRTIRVTVRVIAATNRDLGRMVEEQAFRSDLYYRLKVFPVTVPPLRERVEDIPLLVR
                     HFAQKFALRMKKRIETIPSEAMQALQAYHWPGNVRELENFIERAVILTQGPDLVVSLA
                     ELKRRPSHTTQSGTTTLEQAEREHILRSLRETNWTIGGR"
     gene            complement(2562..3203)
                     /locus_tag="VLL06_02130"
     CDS             complement(2562..3203)
                     /locus_tag="VLL06_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSS29795.1"
                     /translation="MKIALLGATGFVGSAILKEALDRGHEVTAIVRNPGTLTPHAKLR
                     PMKGDVYNEAEIVRLVAGSDAVISAFNPGWSNPDIYSQQVKGVRSILNGVKNSGVKRL
                     LFVGGAGSLEVTPGVQSVDMPGFPAEYKQGALATRETLNMLRKETGVEWSFLSPSADL
                     FPGPRTGTFRLGTDQLLTNAQGESRISVEDYAMAMIDEVEKPQHIRRRFTVGY"
     gene            complement(3332..3568)
                     /locus_tag="VLL06_02135"
     CDS             complement(3332..3568)
                     /locus_tag="VLL06_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HSS29796.1"
                     /translation="MEKPRIAAKQPAVLQLDPGTYYWCLCGRSRDQPFCDGSHQGTGF
                     EPVEFTLSEKEEVALCQCKQTKTPPFCDGTHATL"
     gene            complement(3590..4036)
                     /locus_tag="VLL06_02140"
     CDS             complement(3590..4036)
                     /locus_tag="VLL06_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSS29797.1"
                     /translation="MKAHYLGHVVFYVKDLQQSLGFYRDLLGFEEVGRIFNGAAAALT
                     SGRTHHELLLIQVGDAPGPPSGMRRGLYHIGIKIGDNLDQLRLAKKELEQAGVTIDGM
                     SDHTVSQSLYLHDPDGNEVELYVDADESVWKNNPAAVVSPIKPLHL"
     gene            complement(4157..4585)
                     /locus_tag="VLL06_02145"
     CDS             complement(4157..4585)
                     /locus_tag="VLL06_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSS29798.1"
                     /translation="MAVQVYGCNHIVIEVTDAKKAVKFYSDVFGLKMLRGGEGAAWCK
                     LGEHQFMAIFEVETLQPDRVKHFGLMVRDAQQIKEVRRKLTKKYKLTMQPHFRCDFRD
                     PWGNRIQVGDLSDESLIWLLPYQEVQKAGVTFTSRPQKGK"
     gene            complement(4585..5253)
                     /locus_tag="VLL06_02150"
     CDS             complement(4585..5253)
                     /locus_tag="VLL06_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HSS29799.1"
                     /translation="MKESGMRVEIYSDMVCPWCFIGKRRWEHAMETAGYANHVQVTWR
                     PFQLNPTMPKSGMDRRTYIEAKFGGAEGRREIEERLAKAGVADGIMFAFDRIARTPNT
                     FEAHRLMWFSQQHKKQDDLAEGLFHAYFSEGRDIGEGQTLVDIAAEIGLDRDTTRQFL
                     ASDQGVEVVREEEATGHGLGIRGVPYFVLNGTYAISGAQSPDIFVSALQKVEADCTDG
                     KAGR"
     gene            complement(5268..6152)
                     /locus_tag="VLL06_02155"
     CDS             complement(5268..6152)
                     /locus_tag="VLL06_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HSS29800.1"
                     /translation="MPLTNVRNTQEMKVYQPGSQHVVGDGFHVRNLFPSNDLDRALSP
                     FIMLDYAGPTFYPATDTPRGVGEHPHRGFETVTIVYQGMLAHRDSAGNSGVIGPGDVQ
                     WMTAASGIVHEEFHEREWAKRGGTLQAIQLWVNLPKSSKMSAPGYQTIVDEQIPVVEL
                     DGGAGSLRVIAGSLGGAKGPAKTFTPIELYDVRLRAGRSMPLQFPKGYNVGLLILSGQ
                     ASVEGSQRLSEAELGVFVSTGEPVTVSAEEDGTILVMAGEGIHEPVARYGPFVMNTKA
                     ELVQAVNDYQAGKMGHLD"
     gene            complement(6403..6885)
                     /gene="smpB"
                     /locus_tag="VLL06_02160"
     CDS             complement(6403..6885)
                     /gene="smpB"
                     /locus_tag="VLL06_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HSS29801.1"
                     /translation="MGKEKDSFAKAVVTNRKAYHDYFIEEKFEAGIMLQGTEVKSIRD
                     GRVNLQDSYASVKGGEAFLHHCHISPYSHGNIMNHEPLRTRKLLLHRKEINKLLAKTQ
                     QQGLTLIPLRIYFSKRGLAKVELGLAKGKKQHDRRESIKNREASREVERAMKGSRRDR
                     "
     gene            7000..7500
                     /locus_tag="VLL06_02165"
     CDS             7000..7500
                     /locus_tag="VLL06_02165"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HSS29802.1"
                     /translation="MRRLGSIDSVSSVLEGLARRLGLESKMLESRLRRDWVAIVGEPI
                     ASNTWPDQIRYKKLYLVVHNSVWLHQLTFLKPTLIHNINGVDGGELVTDIVFRVGELP
                     ETGHGPGMTETLCATALPMSDALLAEISVHVTAIQDPLLRDQLTQLMAQALTQPRAPK
                     AGSVDP"
     gene            complement(7442..7984)
                     /locus_tag="VLL06_02170"
     CDS             complement(7442..7984)
                     /locus_tag="VLL06_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29803.1"
                     /translation="MDTVVKLGLGVLVGSFSLIWGGMYLTRPDRSIPPYSVGSQEGTA
                     VAVHVPDGTSDHGIETLIERFRKIGRESRKFEPMKINPTTPDRPGDHYRRLTIYIFSD
                     NTWTEPAILHKFLTGEDRGGFQRSLRGLYRLTESEEEGRIGPLLDTPDTAATQASARQ
                     LFKGPLSLPSAPAAESTPAP"
     gene            complement(8092..>10473)
                     /gene="gyrA"
                     /locus_tag="VLL06_02175"
     CDS             complement(8092..>10473)
                     /gene="gyrA"
                     /locus_tag="VLL06_02175"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HSS29804.1"
                     /translation="IVGRALPDVRDGLKPVHRRILHGMNEMGLASNRAYRKSAKIVGE
                     IMGNYHPHGDSAIYDTLVRMAQPFNMRYMLVDGQGNYGSVDGDPPAAMRYTEARLTKV
                     AEDMLADIDKETVDFGPNYDESLVEPLVLPSKIPNLLVNGAGGIAVGYATNIPTHNIR
                     EVIEGLLLFLESPDITTSQLMKKIPGPDFPTAGFIYGTAGIKSAYESGRGLLTLRAEV
                     KIESDERTERERLIVTELPYQVNKSKLIEKIAELIRDERIKGISDLRDESDRDGIRVV
                     IELKRGEIPLITLNNLYKLTQLQTTFGVIMLALVNNRPEVLSLKQILHHFVEHRREVI
                     VRRTAFELRKAEERAHILEGLKIALDNLDAVITLIRRAQSPDEARAGLMRQFALTEIQ
                     STAILEMRLQRLTQLERNKLIEEYTEVLKQIERLKSILGSDALVRTIVKDELIEVREA
                     YKDDRRTQIVKEEAEITLEDMIANEEVVVTISHAGYIKRNPVTLYRAQRRGGKGKIGM
                     GVKEEDFVVTLFTATTHDSLLFFTDAGKVYWLKVHEIPDAGRAAKGKALVNLLALSSA
                     EQVTATVPVKEFREDRFVVMATAQGIIKKTELSAYSNPRQGGIIALSLDKGDRLISVH
                     VTDGQREILLGTKKGITIRFKEDDVRSMGRTAHGVRGITLEEGNEVIGMETITPDSTT
                     QILTVTEGGYGKRTPVNEYRVQGRGGKGIISVKTTERNGLAVGFLQVRDGDEIMLMAA
                     QGKVLRCKVDEFREIGRNTQGVRILDLEGAGDRVVAVARLAESAEVLPEEGGG"
BASE COUNT         2130 a         3160 c         2771 g         2412 t
ORIGIN      
        1 atctaccgcc gatggtccaa ttcgtctcac gaagtgacct gaggatatgc tcgcgctcgg
       61 cttgttcaag agttgtggtc ccagactgtg tggtatgact cggcctccgc ttcagttcgg
      121 cgagcgacac cacgagatcc ggcccttgag taagaatcac cgctcgctca ataaaattct
      181 ccaactcccg cacgttgccg ggccaatgat aggcttggag cgcttgcata gcctccgacg
      241 gaatcgtctc gatgcgtttc ttcatacgta gggcgaattt ctgagcaaag tgtcgaacca
      301 acaagggaat atcctccacc cgttcccgca ggggaggaac ggtgacaggg aagaccttga
      361 gccggtaata caagtcactg cgaaacgcct gctcctccac cattcgaccc aggtcgcggt
      421 tcgtggccgc gatcacgcga accgtgacgc gaatcgttct ggtgctcccc aatcgttcga
      481 actcctgttc ctgcagcacg cggagcaact tgacttgtaa ttccaacgga atctccccca
      541 cctcatcgag aaagatcgtg ccgcggtccg ccaattcaaa ccggccgatc ttggcggcaa
      601 tcgcgccggt aaatgcacct cgttcatgtc cgaagagttc actctccaat agccccgtcg
      661 gaatcgcggc gcaattgagc ttgacgaacg tgcgttccct ccgatcgctg cgattgtgga
      721 gcgcccgcgc gaccagttcc tttccactgc cggtttcgcc gaggatcagg acggtcgaat
      781 ccgttgccgc gacagtctgg atttctttca gaagcagctt gagcgcccgg ctgtcaccga
      841 cgatttcctc gaagttgtat tccgtctgga tttcctcctc caggtacagc ttttcttttg
      901 ccagcttgtc cttcagcgat gagatttcct gataggccat ggaatttgca gtggcgagtg
      961 caacttgtgc gccgatccag ctcagcaatt tcgcatcgtc ttcggtgaac gcactctcct
     1021 gcttactgac gacactcagc gtgcccagtc ctcgcccgtg cgagagaaga ggaacggcgc
     1081 aaccggattt caggcccgcg gcggcacccc gcttcatgat ctccgcagga aactcggaga
     1141 ggtcgaggct tctgatcagc acggtctgct gagtcgtgaa cgccaaccct tccggagtcc
     1201 cttccaacgg aattggatct cccgcttcgg taaagtcttg atggctgggg aagtccagcg
     1261 catgagtttg caattgatga gtcgcgggat cgtagagaca aaaccaggcg agatcgtgcg
     1321 ggatcacacg tccaaggcag ccggagatcg ctttcaggag ttttggcaat tcgaggtgtg
     1381 acacgacggc gttgttgatc tccagcacca agctgagccg atctcgttcc cgagccaatg
     1441 actgctgcgc ggcttccgtg ccggcgaaat tgagcacgtt gtccacggcc acggcaacct
     1501 gcttggccac ctgaccgagt aaccccaggt tcttctctcc gaatgcacgg gtttcgagac
     1561 ttccgaatcc gatcgcgccg aggcgccgca tggcagttgt caacggaaac aggcagacag
     1621 attggacacc aatgtctttg agcgccgcca tcccctttgt aaatcgagtt tcctcagcga
     1681 gagacggaat gatcaagggc tgctggtggg tccagaccca tccgctggag gactcctcga
     1741 tcgggagttc caatccatcc agtttggtgg tgaggtggtg tgccgctgct gtttccaata
     1801 catggacctt catcagattc tttgcgggat cgtggaggat caacccgata aagtcgaacg
     1861 cagcgattgc cgggaggcgt tgggcgagat cccgaaagag cgaggacagg tcgcgatgtg
     1921 acacgatggc ttcggacact tccaggagca gccgtaaccg gtcacgatcg tgaatcaggt
     1981 cttgatgatg gaggacattg tccaccgcca cggcgatttg tttggccact tgctggagaa
     2041 actccaggtc cctttcacca taggcatcct tctccaggct caaaaatccc atcgcgccca
     2101 gccgccgcac cgcggtggtc aggggaacaa cgcaaaagga gttcacgccg tcctcctgca
     2161 tgcattgagt gaccttcggc cagcgacctt cttctataat attggcaaca aggatcggct
     2221 gttgcgtgtg ccacaccaat ccatcaggag tttcttcgac cggtccttca tggccgccca
     2281 cgagatcagc tggcacattc gcctggatcg tgtgcaatcg catcgtgttt cgaacgggat
     2341 cgtgtaagga cagggcgaca aaattgacgt gcaccactcg cggaaggcgt tgggcaagat
     2401 ctcgaaagag ttcgtgcaga tcgcggtgcg aggagatcgc ctctgcaacc tctagcaggg
     2461 ttcgatattg ctccgccagg gtctcgcagg gagatgccga gtgcttattc atagccgttt
     2521 agtatgggct cttggtccgc aacgactcaa gatttcccct cttaataacc cacggtaaag
     2581 cgccggcgga tatgctgtgg cttctccacc tcatcgatca tcgccatcgc atagtcttcg
     2641 actgaaatgc ggctttcccc ttgagcattc gtcagtagct gatcggtccc gagtcggaat
     2701 gtaccggtgc gcgggccggg aaagaggtcg gctgacggag agaggaacga ccattcaacg
     2761 cccgtctcct tccgaagcat gttcagagtc tcccgcgtcg ccagcgcacc ctgcttatat
     2821 tcggctggaa agccgggcat gtccacggac tggaccccag gcgtgacttc caaactgcct
     2881 gccccgccta cgaacaacaa ccgcttcaca ccggagttct tgactccgtt gagaatggaa
     2941 cggacgcctt tgacctgctg gctgtagatg tccggattgc tccatcccgg attgaacgca
     3001 ctgattaccg catcggaacc cgcgaccaaa cggacgattt cagcctcgtt gtagacatcg
     3061 cctttcattg gacgaagctt cgcgtgtggt gtcagtgtgc ccgggtttcg cacgatggcc
     3121 gtcacctcgt ggccacgatc caacgcttcc tttagtattg ccgacccgac aaagcctgtc
     3181 gcgccaagca atgcgatctt cactgtgtcg cccctctcag atcgaaaggc gtgatcggac
     3241 agatctgtca cgcgaactgc gatgacaatc cagtgtgtac cgcctgaccc gatgcgaaac
     3301 ctacggaagg tatgcgagac ttccggcgta atcagagcgt cgcgtgcgtg ccatcgcaga
     3361 acggcggggt cttcgtctgc ttgcactggc acaatgcgac ttcttccttt tcactcagtg
     3421 taaactcgac cggctcaaac ccagttcctt gatgggaccc gtcacagaac ggttggtccc
     3481 tcgagcgccc gcatagacac cagtagtatg ttcccgggtc taactgaagc acggcgggct
     3541 gcttggcggc aatacgtggc ttctccatga aggggcctcc ttgtgtacgt tacagatgta
     3601 acggtttgat gggcgacacc acggccgccg gattattctt ccacaccgac tcgtctgcat
     3661 cgacatagag ttccacttcg ttgccgtccg gatcatgcag atagaggctc tgactcaccg
     3721 tatgatcgct catgccgtcg atcgtcacgc ctgcctgttc caattctttc ttcgccagac
     3781 gcaattgatc cagattgtct ccgatcttaa tgccgatatg atagagccct cggcgcatcc
     3841 cggacggagg acccggcgca tcgccgactt ggatcaataa caactcatgg tgtgtgcgac
     3901 cggacgtgag agccgcagcg gccccgttga agatccgccc aacttcctcg aaccccaaca
     3961 gatcacgata aaaaccaagt gattgctgca gatctttcac atagaagacc acgtggccga
     4021 ggtaatgcgc tttcatggct tgattcctct tatattgcac gcgtcactgc tgcttactgg
     4081 tcctcgacga gcttgcctgg acgaatgtac ccgaacagat cattcagtcg ctgctgtaga
     4141 ttgacggtac tcatgatcat ttgccctttt gtggccggct ggtaaatgta actccggctt
     4201 tctgtacttc ctgataaggg aggagccaga tgagcgattc atcgctaaga tcaccgacct
     4261 gaatacggtt tccccaagga tcgcggaagt cgcaacggaa atgtggctgc atcgtgagtt
     4321 tatacttctt cgtgagcttc cgtcgcacct ctttgatctg ctgtgcatca cggaccatca
     4381 ggccaaaatg tttcacgcga tccggttgca gtgtctcgac ttcaaagatg gccatgaact
     4441 gatgttcgcc caacttgcac caggcggcgc cttcaccacc gcggagcatc ttcaacccga
     4501 agacatccga atagaacttg acggccttct tggcgtcggt gacttcgatc acgatgtgat
     4561 tgcatccata gacctggacc gccattatcg acctgccttt ccatccgtac aatcagcctc
     4621 taccttctgt aaggccgaga caaagatgtc cggagattgc gcgccggaga tcgcgtacgt
     4681 gccattcagc acgaaatagg gaactccccg aatgcctaaa ccgtgccccg tcgcttcttc
     4741 ttcccggacg acttcaaccc cctggtcact cgcgagaaac tgtctggttg tatcgcggtc
     4801 gagcccaatc tctgcggcga tgtcgacgag tgtctgcccc tcgccgatgt cacggccttc
     4861 cgagaagtag gcatgaaaca gcccttcggc aagatcgtct tgtttcttgt gctgttggga
     4921 gaaccacatc aatcgatggg cctcgaaggt attcggggtc cgcgcaattc tatcgaaggc
     4981 aaacatgatc ccgtctgcca caccagcctt ggcgaggcgc tcttcgatct cccgccggcc
     5041 ctcagcgccg ccgaatttcg cctcaatata tgtgcggcga tccataccgg acttcggcat
     5101 cgtcggattc aactgaaacg gtcgccaggt cacctgtacg tggttcgcat acccggcggt
     5161 ctccattgcg tgttcccatc gacgcttccc gatgaagcac caggggcaga ccatatcgga
     5221 atagatctcc acgcgcatcc cggattcctt catgctctgc ccctggttca atccaaatgc
     5281 cccatcttgc cggcctgata atcgttcacg gcctggacca gttctgcttt tgtattcatc
     5341 acaaagggtc catagcgagc cactggctca tggattcctt cgcccgccat cacgagaatc
     5401 gtgccatcct cctctgcact gaccgtcacc ggctctcctg tcgagacaaa gacgcccagt
     5461 tcggcctccg ataacctctg cgatccctcg acggaggcct gaccactgag gatcaacaga
     5521 ccgacattgt accccttcgg gaactgcaac ggcatcgaac gtcccgcgcg cagacgcaca
     5581 tcgtacaatt cgatgggcgt aaacgtcttc gccgggcctt ttgcgcctcc caacgacccg
     5641 gcaatcacac gaagcgaacc ggctcccccg tcaagctcca cgaccggaat ctgctcgtcc
     5701 acaatcgtct ggtaaccggg cgcggacatc ttggacgatt ttggcaaatt gacccacaat
     5761 tgaattgcct ggagggtccc gccacgcttc gcccattcgc gctcatgaaa ctcttcatgg
     5821 acgatacccg acgccgcggt catccactgc acatcgccgg gaccaatcac cccggaattg
     5881 cccgccgaat cacgatgcgc caacatgccc tgatatacga tcgtcacggt ttcgaaccca
     5941 cggtgtggat gttcgccgac ccctcgtggg gtatccgtcg ccggatagaa cgtgggccct
     6001 gcatagtcca gcatgataaa cgggctcagc gctcggtcga gatcgttact cggaaacaga
     6061 ttccgcacat gaaatccgtc cccgacgacg tgctgagagc ccggttgata aactttcatc
     6121 tcctgcgtgt ttcttacatt ggttaacggc atcgtgtctc tcattggacc ctctctttct
     6181 cccccacggt ccccaggcag cctcgcctgt ccggcgtggg gggttcgtgt tcgccctcta
     6241 ttatcgggca cctatcccaa tccaatctcg tagctccgtg accccatgta taataatcct
     6301 agttagaact aagtcaacag ggaaacaccg cgctggtatt ctttcgtaaa atactccgtg
     6361 aggtcaatcg gttcgagtga ggtgggataa caacacggac gattatcgat cgcgccgcga
     6421 ccctttcatc gcccgttcca cttctcggct ggcttcgcga ttcttgatgg attcccggcg
     6481 gtcatgctgt ttcttgccct tggccaaccc gagctcaact ttggcaaggc cccgtttcga
     6541 gaaataaatg cggagcggga taagcgtgag cccttgctgt tgggtcttgg cgaggagctt
     6601 attgatctcc ttgcggtgga gcagcagttt ccgagtacga agaggttcgt gattcatgat
     6661 gttgccgtgg ctgtagggac tgatgtggca atggtgaaga aacgcctcgc cgcctttcac
     6721 gctggcgtaa ctatcttgta gattgacacg cccatcacga atggatttga cttcggtacc
     6781 ctggagcatg atacctgctt caaacttctc ttcgatgaaa tagtcatggt aggccttgcg
     6841 attggtgacg accgctttcg caaaactgtc cttttctttt cccatcagcc gtcagacttt
     6901 caattccaga atttagtcct gtcagcacac cgtccagaat agactgaccg agaactcact
     6961 gcagattgcc acggcggctt ttgctattat gatgtcgcca tgcgtcgact tggttccatc
     7021 gattctgttt ccagtgtcct cgaagggttg gctcgtcgct tgggcctcga atcgaagatg
     7081 ctcgaaagtc gcttgcgccg ggactgggtc gccattgtcg gagaacctat cgcatccaat
     7141 acctggcctg accaaattcg ctacaaaaaa ctctatctcg ttgtccacaa cagtgtatgg
     7201 ctgcatcagc tcaccttcct caagccgaca ctcatacaca acatcaacgg tgtcgacggc
     7261 ggagaactcg tcaccgacat cgtgtttcgc gtgggagagc tgccggaaac cggccacgga
     7321 cccgggatga cagagaccct ttgtgcgaca gctttaccaa tgagcgacgc gttgctcgcg
     7381 gaaatttcgg tccacgtcac cgcaatccag gatccgctcc tccgcgatca actcacccaa
     7441 ctcatggcgc aggcgttgac tcagccgcgg gcgccgaagg caggctcagt ggacccttga
     7501 aaagttggcg ggcactcgct tgcgtcgccg ccgtatcggg agtgtccagt agcggaccaa
     7561 tccgtccttc ttcttccgat tcggtcagtc tgtacagccc cctcaacgac cgttgaaatc
     7621 ctccacgatc ctccccggtc aggaacttgt gcaggatcgc cggctccgtc caggtattat
     7681 cggaaaagat atagatggtg aggcggcggt agtgatctcc cggcctgtct ggcgtcgtcg
     7741 gattgatttt catcggttcg aactttctcg actcgcgacc tattttgcga aagcgctcga
     7801 tcaacgtttc gattccgtgg tcgctcgtgc cgtcgggaac atggaccgcc acggctgttc
     7861 cttcttgaga tccgacgcta tagggaggga ttgatcgatc tggacgggtc agatacatcc
     7921 caccccagat caggctgaag gacccgacga gtacgccaag acccaacttg acgaccgtat
     7981 ccatgcgctt cttgggcttg gatcgcggga ggggcgttgg agaaggatcc tgctcgtgca
     8041 tggaaggact gcgttcagcg aaccaggtgc atgatgccgc gcgtatctgt tttacccgcc
     8101 gccttcctcg ggaagcacct cggcactttc cgccaaacgc gcaacggcca cgactcgatc
     8161 acccgcgcct tcgagatcga ggatgcgaac cccctgagtg tttcggccga tctcgcggaa
     8221 ttcatccacc ttgcaccgaa gcactttgcc ttgagccgcc atcagcatga tctcatcacc
     8281 gtctcggact tgaagaaacc caacggccag tccattccgt tcagtcgtct tcacgctgat
     8341 gatgcccttc cctccgcgtc cctggacccg atactcgttc accggcgtgc gctttccgta
     8401 accgccttcc gtgaccgtga ggatttgggt ggtggagtca ggggtgatgg tttccatgcc
     8461 gatgacttcg ttgccttcct caagtgtaat gccgcgcacc ccgtgcgccg tacgtcccat
     8521 agaacggacg tcgtcttcct tgaagcggat cgtgataccc tttttcgtcc ccaacaggat
     8581 ttcccgctgg ccatcggtga catgcacgct aatgagccga tcccccttat ccaacgacag
     8641 tgcgatgatc ccgccctggc ggggattgct gtaggccgag agttcggtct tcttgatgat
     8701 gccctgcgcg gtcgccatca cgacgaaccg gtcttcgcgg aactctttca ccggcacggt
     8761 cgcggtgacc tgttcagcgc tggagagtgc caacagattc accaacgctt tgcccttggc
     8821 agcccgtcct gcgtccggaa tctcatgaac tttcagccaa tagacctttc cggcgtccgt
     8881 gaaaaacagg agcgaatcgt gcgtggtcgc ggtaaagaga gtcacgacaa aatcctcttc
     8941 cttcacgccc atcccgatct tgcccttgcc gccgcgtcgc tgcgcccgat acagggtcac
     9001 tggattgcgc ttgatatagc cggcgtgcga aatcgtgacg accacttctt cattcgcgat
     9061 catgtcttcc agggtaatct cggcttcttc ctttacgatc tgcgtgcggc ggtcgtcctt
     9121 ataggcttca cgcacctcga tcaattcatc cttgacgatc gtacgaacca gcgcgtcgct
     9181 ccccaggatc gacttcaaac gctcgatctg tttcaagacc tcggtatatt cctcgatgag
     9241 tttattgcgc tccaattgcg tcagccgctg aagccgcatc tccaaaatgg cagtcgactg
     9301 aatttcggtc agtgcgaatt gtcgcattag accggctcgg gcttcatcgg gcgattgggc
     9361 ccgtcggatc aaggtaatca ccgcatccag gttgtcgagc gcgatcttga gaccctcgag
     9421 gatatgggcc cgctcttccg ccttacgcag ctcgaacgca gtccgacgga cgatgacctc
     9481 acgccggtgc tcgacgaaat gatgcaggat ctgcttgaga ctcaatactt ccggccggtt
     9541 gttcaccagc gcaagcatga tgaccccaaa ggtcgtttgg agttgtgtca gcttatacag
     9601 attgttcaac gtgatgagcg gaatttcccc gcgcttgagc tcaatgacga cgcggatgcc
     9661 gtcccgatcg gattcgtcgc gcagatcgga aatccctttg atccgttcgt ctcggatcaa
     9721 ctcggcgatc ttttcaatga gcttggactt attgacttga tagggcagct ccgtgacgat
     9781 cagccgttcg cgctccgtgc gttcatccga ttcgatcttc acttcggcgc gcaaggtgag
     9841 gagaccccgt ccgctttcgt aggcggactt gatcccggct gttccataaa taaatccggc
     9901 tgtcggaaag tcagggcctg gaatcttttt catcaactgg ctggtcgtga tgtccggact
     9961 ctccaggaac aagaggagcc cctcgatcac ttcgcggata ttgtgggttg gaatgttcgt
    10021 ggcatagccc accgcaatgc caccggcgcc gttcacgaga agattcggga ttttcgaggg
    10081 gagcaccagt ggttcaacca gcgactcgtc gtagttcggc ccgaaatcga ctgtttcctt
    10141 gtcgatatcg gcaagcatgt cctcggcaac cttggtcagg cgggcttcgg tatacctcat
    10201 ggctgccggc ggatccccgt cgaccgatcc gtaattgccc tgaccgtcca cgagcatgta
    10261 gcgcatgttg aagggttgcg ccatccgaac gagcgtgtcg tagatcgcac tgtcaccgtg
    10321 ggggtgatag ttccccatga tctcgccgac gatcttggcg gacttccgat aggcacggtt
    10381 cgacgccaag cccatttcgt tcatgccgtg gagaatccga cgatggaccg gcttcagtcc
    10441 gtcacgcacg tcgggaagtg cccggccgac gat
//
LOCUS       DATIPF010000086         5953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_545685, whole genome shotgun sequence.
ACCESSION   DATIPF010000086 DATIPF010000000
VERSION     DATIPF010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545685"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            414..1205
                     /locus_tag="VLL06_02180"
     CDS             414..1205
                     /locus_tag="VLL06_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29805.1"
                     /translation="MASSSEIVGFCLLSLATPAFAGNQIYSYTDDSGALNFTTERDRI
                     PEQYRNQAVLLKLDVSPPVETPPVQPRLRVVTSSSEYRMSDHDTRIDATRLAIDDAKR
                     QALEQIAVYLESITQVKDLDVTYDEIRAYTAGIVMVLKQQTSTRLEDGEIVIHVDLTA
                     QIDEHDVIQAIASLRENESAKQELVSLRTKTDQLRQQLDAANQALATANTPAQIQALT
                     IQRQRLLDRMQADALSAQGKAEKAALATETSSPSMARLKRFLGAP"
     gene            2020..2334
                     /locus_tag="VLL06_02185"
     CDS             2020..2334
                     /locus_tag="VLL06_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29806.1"
                     /translation="MALLIILIFCLLPHSAQAQSVEAPLFAHSARIYIVYPMPDVVYF
                     HDEHGNSVIFYRQWSGLAWYSARDSSGRIVREGYGIAPPLALNPSSVPSLNRETSDED
                     VR"
     gene            complement(2784..3899)
                     /locus_tag="VLL06_02190"
     CDS             complement(2784..3899)
                     /locus_tag="VLL06_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29807.1"
                     /translation="MHTLSVPLLTINKGTCHAVFAYDIGWGIDLESVNRRISADTERS
                     RFRHKTKAPQYFEYRPAPLRLMQEGVSLAVGQYQSSPTVEVMVYDFGAVTITYRFALD
                     GPFENLLELSELLYENAPLLIESRNRVEQLVQTIHPSIERPKIAKEVEDYIIFSIESY
                     SPAQSLPPWTSLKTELAQVLRGERMPLADQEIHDAMSCRISYGVEDATLIDWHSAVIF
                     GKEMDDVRAVLEFANVELLEMRMLDEQLDAALDEGYAALARKPRLLPLPGLHDKDTTH
                     IAQLQVDGALLFERVTNTLKLLGDQYLARVYLFASQRFHLEAWDASILRKLETLESIY
                     GKMSDRATTRRMEVLEWIIIVLIAVSIAVSFFPMGGP"
     gene            4131..4358
                     /locus_tag="VLL06_02195"
     CDS             4131..4358
                     /locus_tag="VLL06_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29808.1"
                     /translation="MEACTREAGAWSETEAGQVILLEDDLRLGAAPPEAAIERVASEI
                     LALGKQLNDARLHGFIHDVMQRQPSNSQATP"
     gene            4793..5248
                     /locus_tag="VLL06_02200"
     CDS             4793..5248
                     /locus_tag="VLL06_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSS29809.1"
                     /translation="MRITADRETAEELTIDVFHDIWRRASGYDSANGTVLGWIMNQAR
                     SRAIDRVRFESRKKRSHTGDLPPLVEAAADPSREIEQRQRDESLRAALAALTPDERQA
                     IEMTFFAEHTYAEAASRLNQPLGTIKTRIRSGLHKLRQKLTAGTGTDEC"
     gene            5238..5813
                     /locus_tag="VLL06_02205"
     CDS             5238..5813
                     /locus_tag="VLL06_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSS29810.1"
                     /translation="MSADQNRCKESELTCAYATQALGAHEAAAAEAHIASCLDCQREV
                     ESLRPVVNRFVSWPTDVLRPPTSLQARLAHRLAEETGTPPVLPSARRWSEPEWEQVAP
                     GIECKLLATDTERHRVSMLVRLAPGASYPGHTHAGVEELHLLDGELWIDERKLFPGDY
                     NYGAPGAGDERVWSETGCTCVLITSTNDTLR"
BASE COUNT         1325 a         1701 c         1656 g         1271 t
ORIGIN      
        1 ggccagaatc cccagggtct tcgtgcacct caaggtgcgt agattctcgc cactcaatcg
       61 ccctctcccg cacgcggcac ctgtcaaacc taggaccgat catggtcaaa tgagcaagcg
      121 aaaagcagct ccgtacggca ttgaaggtgt ttcgtgcggt agtgcgatag cttttccaag
      181 gttggtagct tctcagtagc cggttcgtta agccctcgag ttcgctcagc tattcgtccg
      241 gcctgctgat acactatcag cggaccacgt tggagtagcc gttgttgtag cggtcgtcgc
      301 caactcctgg tttcattggg attcccaaag ggcagcgagt gagcaaatcg ccggtcatga
      361 gtatgctcga tcgccttgac ataatggtat agtgaaagag gggcgcgtat tgtatggcct
      421 cttcttctga gatcgtcggt ttctgcctac tcagcctggc cactcctgcc ttcgccggca
      481 atcagatcta tagctatacg gatgattccg gcgcgctgaa cttcaccact gaaagggatc
      541 ggatccccga gcagtaccgc aatcaagccg tgctcttgaa gctggacgtc tcaccacccg
      601 ttgagacgcc ccctgtacaa ccaaggcttc gcgtagtgac atcctcaagt gaatatcgta
      661 tgagtgacca cgacacgcgg atcgatgcca cacggctcgc cattgacgac gccaaacggc
      721 aggcccttga gcaaatcgcg gtgtatctgg aaagtatcac tcaagtcaaa gacctggacg
      781 tcacgtatga tgagatcagg gcctacacgg cggggattgt gatggtgctc aagcagcaga
      841 ccagcacgcg cctcgaagac ggtgagatcg tcattcatgt cgatctcaca gcacaaatcg
      901 atgaacatga tgtcattcaa gccatcgcct ccctccgcga aaatgaaagc gcgaaacagg
      961 aactcgtctc gcttcgaacg aaaaccgatc aacttcgtca acagctcgat gccgccaatc
     1021 aagccttggc cacggccaat acgccagcgc agatacaagc actgacgatc cagaggcaac
     1081 gactgctcga tcgtatgcaa gcagatgctc tgagtgctca aggcaaagcc gaaaaggccg
     1141 ccttggcgac cgaaacaagc tctccatcta tggcccgttt gaagcgtttc ctgggtgcgc
     1201 catagactga agaacttcgt cggatgtatt caaactgatc cgatgggagc agatcggtct
     1261 tgggagcatc agggtgttca aatctcgcag gtctcacatt cagaatgctt aatctgcctt
     1321 cggatatcct cccaggcgat agggctagag cgcaatacag gtcgtaagag caccggaggt
     1381 gtggtcggca gatccatagc tagggagctt tctagtctcg gtgtccggga gtacgtagta
     1441 cgatccgtcc catgaccaag gctcaggcgg ctctcctcca aaggaagtgg aagaagcatg
     1501 gcgatccacc ctgtgaacat ttaatccagg aattgtctga cacgtcgtct gactttgacg
     1561 atgggcatgt gctgaacgcc tatcattgcc gtgagtgtgg agagctgttt gttcacaggt
     1621 atcaggtata aggcgaaggg cttgcccgat aatccttcga ccgaaccagc gttcatcggc
     1681 gagacgtaga gaattcgatc gggtgaacac tcctcaagga tggtctcctc ccccctcctc
     1741 gggctttcac tagcctcgtg ctcttgcccg tcatgatatc gtggacgcat gggatcaacc
     1801 cttcgaccag aggtgcagga gctcattcgt gtgtgtgaaa acctcgatag ccacgatacg
     1861 gtcctcaccg tccacgagtg tgaagccata ctccgctgtg ccaaagaact tgaaagcaaa
     1921 gtccttccgg atcgtcaaca gtctgaccga ccaataccct atcccacttc tcaccgatag
     1981 actgaccctc agggccgtgg agcagaaatg aagaaaggga tggctcttct catcatactg
     2041 atcttctgcc tactgccgca ctccgcacag gctcagagtg tcgaggcccc gctcttcgcg
     2101 cactccgcca ggatctacat cgtctatccc atgcccgacg tggtgtactt tcatgatgaa
     2161 catgggaaca gcgtcatctt ctatcggcag tggtctgggc tcgcatggta ctccgcacgg
     2221 gattcatcgg ggcgcatcgt gcgggaaggc tatgggatcg ctccaccgct cgcgctcaat
     2281 ccatccagtg tgccgtcact gaatcgagag acgtctgatg aggacgtacg ctagggggtc
     2341 gcactcacgg tcacgatcat cacagtcagc ttgcttgtcg ccaaagatgt gaaggaactg
     2401 attgatttcg catttgcccc caacaatcct cctaacggtc cactcggcgt gtgatgtccc
     2461 gcatcagcgg gggcggtagc cgaaataata cctgtccaat gagtctcccc tcgctttcgt
     2521 ccaatagtag tgtcccgcta ataggctcat gtatatctgg cgagaaatct cggtgtgcgc
     2581 caggcccggc ttcattgtgg taccgacaca accaagggcg ttggcaaccg aaggggaaaa
     2641 catctgccct ccacccaact gatttttagc ctcaagtcag ccgtagtagc ccatgcccgc
     2701 gcacccgtgt gattaaatcc tcccctcacc cagctttgtc agccctcaac ggcactcgaa
     2761 tcgtaggcag tgggcgcgtc tcgtcagggc ccacccatag ggaagaacga aactgcgata
     2821 gagacggcga tcagcacgat gataatccat tcaagcactt ccatgcggcg ggtggttgcg
     2881 cggtccgaca tcttgccgta aatgctttcc aacgtctcca gcttacggag aatgctcgca
     2941 tcccaggctt cgaggtgaaa acgttgggac gcgaacagat agacgcgcgc caggtactga
     3001 tcgcctaaga gcttcagcgt gttcgtcacc cgttcgaaga gcagtgcgcc atcgacttgc
     3061 agttgcgcga tgtgtgtcgt gtctttgtcg tgtaaacctg ggaggggcaa caggcgcggc
     3121 tttcgagcaa gcgcggcata cccctcatct agcgctgcat caagctgttc gtcgagcatc
     3181 cgcatttcca agagctccac attggcgaat tccagcacgg ctcgcacgtc gtccatttct
     3241 tttccgaaga tcacggctga atgccagtcg atgagtgtgg catcctcgac cccgtatgaa
     3301 atgcggcaag acatcgcatc gtgaatttct tggtcggcga gtggcatccg ctcgccacga
     3361 agtacctgcg cgagttcggt ctttagactg gtccagggcg ggagtgactg agccggtgaa
     3421 taagactcaa ttgagaagat gatgtaatct tccacctctt tagcgatctt gggccgctcg
     3481 atcgacggat gaatagtctg aacaagctgc tccacccgat tccgtgattc tattagtaag
     3541 ggtgcattct catagagcaa ctcgctgagt tcgagcagat tctcgaaggg gccgtcgagc
     3601 gcgaagcggt aggtgatggt cacggcaccg aagtcataca ccatcacttc cacagtaggg
     3661 ctggactgat attggccgac tgcgaggctg actccttctt gcataaggcg gagcggagcg
     3721 ggtcgatatt cgaaatattg cggagctttg gtcttatgac ggaagcggct ccgttctgta
     3781 tcggcggaga tgcggcggtt aacactctcc aaatcgattc cccacccaat atcataggca
     3841 aataccgcat ggcaggtgcc tttgttgata gtaagtagcg gtacagaaag agtgtgcatg
     3901 aacgtcactc atcctccgtg aaagagttgt tttaacactg gtttaaagat gagtaggagt
     3961 gtgttggttt ggttagcggc ttattcatct ggatgcagcc aacccattca attaatccaa
     4021 cacctcacca ggggcgtttc tttagagatg aaaccccgaa gaaggttgga tgctctgcaa
     4081 aatggatggg ttgcaatatt aaataaactg tccaccatga aactcgtcgg atggaagctt
     4141 gcaccagaga agctggagca tggtcggaaa ccgaagccgg acaggtgatc ctgcttgaag
     4201 acgaccttcg tttgggagcg gctccgccgg aagccgccat tgagcgagtg gcgtctgaaa
     4261 ttttagcatt aggcaagcag cttaatgacg cccgccttca tgggtttatc catgacgtca
     4321 tgcagcgcca gccgtcgaac tctcaagcaa ccccataacg aattctgcca cgagcttact
     4381 ctttttcctc cagggcctgt cgtatcagga cctcacggag cagtctctcc agctcctggt
     4441 gcatcaattg aatgcttgac ttactccacc ttccgcgaag cctgagataa actttcctag
     4501 gtcgtgatct gcggttttta ggtgagccag atacgcgcac aagaaactcg aaccaccggg
     4561 aaggttgccg gtgggggttg cccttatccg gtcaattcga cgataatccg gtggtgcgct
     4621 tccagtttct ttgttcacca aactctagcc ggctttctta ggaggggatg ctcgtgtctc
     4681 tctccctcaa attctcggcc gaggagagca tgtccaagga acagacacag aagcgaccgg
     4741 ccacactcgg cgacgtgctc tatgccaagt ccaaggctcc ttcacattga tcatgcgaat
     4801 tacagccgac cgggaaaccg cggaagagct caccattgat gtcttccacg acatctggcg
     4861 gcgcgcgtcg ggttatgact ccgcgaacgg cacggtgctt gggtggatca tgaaccaggc
     4921 acgttcgcgg gcgatcgacc gcgtgcgctt cgagagccga aagaagcgca gccatactgg
     4981 tgatctgccg ccgctggtgg aggcggctgc cgatccaagc cgcgagatcg agcagcgcca
     5041 gcgggacgag tcgctgcggg cagcattggc cgcgctgacc ccggacgagc gccaggcgat
     5101 cgaaatgacg ttttttgccg agcatacgta tgccgaagcc gcgtcacggt tgaaccagcc
     5161 gctcggcacg atcaagacgc gcattcggtc aggactgcat aagttgcggc agaagctgac
     5221 tgcaggaacg ggcaccgatg agtgctgatc agaaccgctg caaggagagc gagctcacat
     5281 gcgcatacgc cactcaggcg ttaggggcgc atgaggcggc ggcggccgag gcgcacatcg
     5341 cctcgtgcct ggactgtcag cgcgaggtgg agagccttcg cccagtggtc aatcggtttg
     5401 tctcctggcc caccgatgta ttgcgtccgc cgacttccct gcaggcccgc cttgcgcatc
     5461 gtctcgccga ggagaccggc acgccacccg tgctgccgtc ggcgcggcgg tggtccgagc
     5521 cggaatggga acaggtcgcg cccgggatcg aatgcaagct gctcgcgacc gacacggaac
     5581 ggcaccgcgt gagcatgctg gtgcgcctcg cgcctggcgc gagttacccc gggcatacgc
     5641 atgcgggcgt cgaagagctg catctcctcg acggggagct gtggattgat gagcgcaagc
     5701 tcttccccgg cgactacaat tatggtgcgc ctggcgcggg cgacgagcgt gtctggagtg
     5761 agaccggctg cacctgcgtg ctcatcacca gcaccaacga tacccttcgc tgaagcgccg
     5821 caggcgcagg atgcactcat atggccacca cctaggtgcg cgctgggcat cagcgtgtat
     5881 gtccacgcat agcaagcacc ctcgaaaccg agcataccgc cgaccagcta agcgccctct
     5941 ttcccccgct acc
//
LOCUS       DATIPF010000087         2594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_280555, whole genome shotgun sequence.
ACCESSION   DATIPF010000087 DATIPF010000000
VERSION     DATIPF010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280555"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1071)
                     /locus_tag="VLL06_02210"
     CDS             complement(139..1071)
                     /locus_tag="VLL06_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HSS29811.1"
                     /translation="MQPQLKSKVRCADREVGEVAKVIMDPLSFEVSALVVSMNGAGER
                     QVPIGAVQTVTDNLVQLRSSSSEVMALPPFKREDYVTLHEVEIPGLEKHVHVTPGEVL
                     VPFPELERNVKRRTFFANLTYVTGLFIGLPLVFPVLKYLMKPMYAPMDNRWLRIGNVA
                     KVKANDVGVQFQYKRSIKEAYMPETEIEKNAWVVKATPAVLEKVYQGKDMEFRDTMGK
                     PIWTNKKDTPYLAFSGKCPHLGCAFKWRNHKILGPVFLCPCHLSIYDASGKVLDGPAP
                     RPLDLMPIQVSASGEVQIIDMEFKAGTKSQTRIV"
     gene            complement(1073..1879)
                     /locus_tag="VLL06_02215"
     CDS             complement(1073..1879)
                     /locus_tag="VLL06_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSS29812.1"
                     /translation="MGNVIKKLVIGLVVGGALVGISSALEFPFVFQMLYFGFAMLGAV
                     VFILLDAPPLTTMSGRKSVFALVAFYILLLCTVCIAGASFFPQFDPEDEKGKIAKILK
                     PQIEASEKGKTEELIARAKALDEQVKALEERLKMLSGGQVIAEKPVKGPEPPPIKGSS
                     AVDVMKVGEEQWQLMECYNCHKLKGEGGKKRGPELDNIGTLLTVDEIKEKITDPKSFM
                     AEGFEKEWEKGMMPGKFKDLMEPSDIMALASWLGTFKNTSVKTPRPIKKK"
     gene            complement(1921..>2594)
                     /locus_tag="VLL06_02220"
     CDS             complement(1921..>2594)
                     /locus_tag="VLL06_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29813.1"
                     /translation="ALPFVMVAVMNIYWIPDPMSLAFLVPLILAPFLFGRFIPMTVSA
                     RTVIKIGFLMIIVCDAIWLTPRGFAATGANMAAGLELPSDWDILASMPAKVSAMFSLV
                     FATVVNYFVYNRAIKQGTILWGKIDFTSQFVLIFLAFISIWTMGLMGAVRSLAKKYYH
                     TYGLMPDFTAESFTPTLSYSAWWITVITGVFFTVVSLAVFVALRSSSSKVREPESSPV
                     PVRAK"
BASE COUNT          610 a          759 c          635 g          590 t
ORIGIN      
        1 ggcccagcgg gagccgcccg ggaccgactc attgagcatc ttggcggtca gctgcttcag
       61 ccccacccgc tcatcgataa aggcaaagac cttttctacg accgtaggct gggggccggc
      121 agaaggagtg tcgtgcattt agacaatccg cgtttgggac ttcgtccctg ctttgaattc
      181 catatcgata atttgcactt cgccactcgc ggacacctga atcggcatca agtcgagagg
      241 gcgcggggca gggccatcca gcaccttgcc ggatgcatca tagatgctta agtggcaggg
      301 acagaggaag accggtccaa gtatcttgtg atttcgccat ttgaatgcgc aaccaaggtg
      361 ggggcacttg ccggaaaatg cgaggtaggg ggtatccttc ttattcgtcc aaatgggttt
      421 ccccatggta tcccggaatt ccatatcctt cccttgatac accttttcca gcactgcggg
      481 agtcgccttg acgacccatg cgtttttttc gatttccgtt tcaggcatat aggcctcttt
      541 gatcgagcgc ttgtactgga attggacccc gacatcattg gcctttacct ttgcgacatt
      601 gccgatcctc agccatctgt tatccatcgg cgcatacatc ggcttcatca agtactttag
      661 caccgggaag accagtggca atccgataaa taaccctgtc acgtaggtca agttcgcaaa
      721 gaacgtccgt cgcttgacat tccgttccaa ctcagggaac ggcaccagaa cttccccggg
      781 cgtcacgtgc acatgtttct caaggcccgg gatctccact tcatgcagcg tgacatagtc
      841 ctcgcgttta aacggcggca aggccatcac ttcggacgag gacgaccgca gctgcaccag
      901 attgtccgtc acagtttgca cggcgcctat cggtacttgt cgttcgcccg cgccgttcat
      961 tgacacgacg agagcgctga cctcaaagga cagggggtcc atgatgacct tggccacttc
     1021 gccaacttcc cggtccgcac agcggacctt cgatttcagt tgaggctgca ttctatttct
     1081 tcttaatagg ccttggcgtc ttcaccgacg tatttttgaa tgtacccagc caggaggcga
     1141 gcgccatgat gtcggacggc tccatgagat ccttgaactt gccgggcatc atgcccttct
     1201 cccactcttt ctcaaacccc tcagccatga aactcttggg atcggtaatt ttttccttga
     1261 tctcgtcgac cgtgagtagc gtcccgatgt tgtccaactc agggccgcgc ttctttccac
     1321 cctcaccctt cagtttatga cagttgtagc attccatcag ctgccactgt tcttctccga
     1381 ccttcatcac gtctactgcg gatgaacctt tgataggtgg cggttctgga cctttgaccg
     1441 gcttctccgc tatcacctga cctccgctca gcatcttgag ccgctcctct aacgctttca
     1501 cctgctcatc aagcgccttg gcacgcgcaa tcaattcctc ggttttcccc ttttcagatg
     1561 cttcgatttg gggcttcaaa atcttagcga tcttcccttt ttcatcttca ggatcaaatt
     1621 gtgggaaaaa cgacgcgccg gcgatacaga ctgtgcacaa taagaggata tagaatgcca
     1681 cgagggcgaa cacggacttc ctcccgctca ttgtcgtgag cggcggggca tccagcaata
     1741 tgaatacgac agcccccagc atcgcgaagc cgaagtacag catctggaac acaaagggga
     1801 actcgagggc gctcgaaatg ccgacaagcg caccgccgac cacaagaccg atcactagct
     1861 tcttaatcac attccccatg cagattctcg ttctgtcagc agacgtgctg cttccaattc
     1921 ttatttggcc ctgacaggaa caggactgct ttcgggttcg cgtaccttcg aactggatga
     1981 ccgaagagcc acgaacaccg ccagacttac cacggtaaaa aagacaccgg taatcaccgt
     2041 aatccaccag gcagaatacg acaacgtcgg cgtgaaggac tccgcggtga agtcgggcat
     2101 caaaccgtag gtgtgatagt acttcttcgc cagggaacgg acagcaccca tcaatcccat
     2161 tgtccagatt gaaatgaaag ccagaaaaat cagcacaaac tgggaggtga agtcgatttt
     2221 tccccagaga atcgtgcctt gcttgatggc ccggttatac acgaaatagt tgacgaccgt
     2281 ggcaaacacc aacgagaaca tcgcagagac ttttgccggc atcgacgcca ggatgtccca
     2341 atcagacggc agttccaacc ctgccgccat attggcgccg gtggccgcga atccgcgggg
     2401 agtgagccag atcgcatcac acacaatgat catcaagaag ccgattttaa tgacagtcct
     2461 cgcagagacc gtcatcggaa tgaatcgacc gaaaagaaac ggtgccagaa tcaacggcac
     2521 aaggaacgcc aacgacatcg ggtcgggaat ccaatagata ttcatcaccg cgaccatgac
     2581 aaagggtaac gcca
//
LOCUS       DATIPF010000088         2203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_102419, whole genome shotgun sequence.
ACCESSION   DATIPF010000088 DATIPF010000000
VERSION     DATIPF010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102419"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1328
                     /locus_tag="VLL06_02225"
     CDS             <1..1328
                     /locus_tag="VLL06_02225"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HSS29814.1"
                     /translation="RQMPLNFYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDQDEAS
                     ARLSYQKMYDAYHRIFTRCGLTFRAVEADTGLIGGSSSHEFMVLAETGENTIVYSEFG
                     TYAANVEQAEVLPPTDVDTDTPRPLRTVQTPQCRTVEEVTSFLKISPTHLVKTLLYTT
                     GNNTVAVLIRGDHEANEVKIQRLLRVTDLELATPTVVEHVTGAPLGFAGPVGLKQVRI
                     IADHAVKALHNMVVGGNQRDTHYVDANWDRDFQVEQFADLRIACAGDPSPRRDGTLKA
                     TKGIEVGHVFMLGTKYSDAMKASFLDLHGKECLAVMGCYGIGVGRTAASAIEQNHDAK
                     GIIWPFPIAPFHVHLLPLTQSTQTAQVTAQLYDELQAAGLEVLWDDRDERAGVKFNDA
                     DLMGAPFQVVIGDKGLAEGVVEVKTRKDGIKLKLPPAQVAAHLTRTQAT"
     gene            1511..>2203
                     /locus_tag="VLL06_02230"
     CDS             1511..>2203
                     /locus_tag="VLL06_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSS29815.1"
                     /translation="MQAKPIPQNLQELQQKVAFAENVKRITEQIHAASDLDHILLDLR
                     KEILSIFDAEDLTIFAFDSEKKEIFSKVPHIDSVEEIRIPITEQSLAGFCAKYLRPVS
                     IADAYNLAELQGVHPSLLHDTSYDKRTGFRTKQVLTYPIVADNKYLMGVLQLLNKKSG
                     GRFTRKDEESVAEISKALGIALFNLRKVAKKAPTKFDLLVTNSRITQNELDHAIAESR
                     KGVSDFESILIEK"
BASE COUNT          554 a          650 c          552 g          447 t
ORIGIN      
        1 atcgacagat gccactgaac ttctatcaaa ttcagacaaa gttccgcgac gaaatacgcc
       61 ctcgcttcgg actcatgcgg ggccgtgagt tcatcatgaa agacgcctat agctttgacc
      121 aggacgaagc aagcgccagg ctcagctatc aaaagatgta cgacgcctac catcgtattt
      181 tcacgcgttg cggcctcacc ttccgtgccg tggaagcaga cacaggcctg atcggcggta
      241 gctcgtcgca cgaatttatg gtgctcgccg agactggcga gaacaccatc gtctacagcg
      301 agttcggcac ctacgccgcc aatgtcgaac aggccgaagt ccttccacct actgatgtgg
      361 acaccgacac gccccggcct ctgcgcaccg tccagacacc ccaatgccga acagtcgaag
      421 aggtcacgag tttcttgaag atttccccca cgcacctcgt aaagacgctc ctctatacga
      481 cagggaacaa cacggtcgcc gtgttgatcc ggggagacca tgaggcgaat gaggtcaaga
      541 ttcaacggct cctgcgagtg actgatctcg aactcgccac tcccaccgtt gtcgaacatg
      601 tcactggcgc accgcttgga tttgccggtc ctgttggctt gaaacaggtc cggatcattg
      661 cggaccacgc ggtcaaagcc ttacataaca tggtcgtcgg cggtaatcag cgtgatacac
      721 actacgtcga tgcgaattgg gatcgtgatt ttcaggtcga acagtttgcc gatctccgga
      781 tcgcctgcgc aggagatccg tcaccaagaa gagacgggac attgaaggcg acgaaaggca
      841 tcgaagtcgg gcatgtgttt atgcttggca ccaagtacag cgacgccatg aaggcctcct
      901 tcttggatct tcacggcaaa gaatgcctcg ccgttatggg atgctacggc atcggtgtcg
      961 gacgcactgc ggcatcggcg atcgaacaga accacgacgc gaaaggcatt atctggccct
     1021 tcccaatcgc cccattccac gtgcatcttc tcccgctcac tcaatcgacc cagactgccc
     1081 aggttacggc gcagctctat gacgaactgc aggccgcagg gctagaagta ctctgggatg
     1141 atcgagacga acgagctggc gtgaagttca acgatgcgga tctgatggga gcccccttcc
     1201 aagtcgtcat cggagacaag ggcctcgccg aaggagtcgt ggaggtgaag actcggaaag
     1261 atggaataaa actcaaactg cctcccgctc aagtcgccgc gcatctcact cgcacacaag
     1321 caacctaggc cccctacctc tgtagcgatg ccagtctgaa agcgcctttt ccttgccttc
     1381 catccatgat cagctagact gactttcgat cactgtctag gcacttgtgg tcacctggat
     1441 cgtacacatg cgtgattgag catacgaatc cggcgacgac cttcccggtc gatgttcagg
     1501 agcccatgtc atgcaggcca aacccattcc acagaacctt caagaacttc agcaaaaagt
     1561 ggcatttgcc gaaaatgtca aacgcatcac ggaacaaatt cacgcggcca gcgatcttga
     1621 ccatattctc ctcgacctgc gtaaagaaat tctcagcatc ttcgacgcgg aagacctgac
     1681 catcttcgcg ttcgattcgg aaaagaaaga aatcttttcc aaagtccctc acatcgacag
     1741 cgtcgaggag atccgcatcc ccatcaccga gcaaagcttg gctggtttct gcgccaagta
     1801 tctccgccca gtgagcattg ccgatgcgta caacctcgcc gagttgcaag gcgtccatcc
     1861 ctcacttctc catgacacat cctacgacaa gcgaaccggt ttcaggacaa aacaggtcct
     1921 cacctaccca attgtggcag acaacaagta tttgatgggc gtgttacaac tgctcaacaa
     1981 gaagagtggc ggacgcttca cgcgcaaaga tgaagaatcc gtcgcggaaa tttcgaaggc
     2041 actcggcatc gccctcttca atctgcgcaa ggtcgctaaa aaagccccga ccaagtttga
     2101 tctcctggtc acaaacagcc gcattacgca aaacgagttg gaccatgcga ttgcggagtc
     2161 caggaaaggt gtgtcggact tcgagagtat cctgattgaa aag
//
LOCUS       DATIPF010000089         5077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_546012, whole genome shotgun sequence.
ACCESSION   DATIPF010000089 DATIPF010000000
VERSION     DATIPF010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546012"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /gene="tmk"
                     /locus_tag="VLL06_02235"
     CDS             <1..453
                     /gene="tmk"
                     /locus_tag="VLL06_02235"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HSS29816.1"
                     /translation="LLIFAARCQHVTQLVIPALRQDTVVLCDRFSDSTFAYQGFARGL
                     DLQWLRKVNEVATGGLTPDLTLVLDLPVSVGLARRRADIGQQNRLDRETERFHRKVRR
                     GFLALAAEEPNRMTIVNANRPAQEVRDELTEIVLGWMARPRRSTRRRR"
     gene            455..1483
                     /gene="holB"
                     /locus_tag="VLL06_02240"
     CDS             455..1483
                     /gene="holB"
                     /locus_tag="VLL06_02240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HSS29817.1"
                     /translation="MPFRDCIGHHTSIAFLQSAVTHERLAHAYLFHGEDAIGKRLTAI
                     RLAQALNCERPPEADDLDSCGACRSCRQIEALTHPDFFVIEPDPEQATQQIKIEQVRE
                     IEHQIMYRPLIGERKICLIDNADRMTIGAANALLKTLEEPPAHSLFLVISSRPAALPA
                     TIRSRCQALRFTTPARTQVEAALILKRELPPTEARFLAIISEGRIGEALTADVKDTRA
                     RQQELVELVRPQSLQSIGSILSSAEAIAKADRAQDMLGWLARWIRDLVLIQVGGDRDQ
                     LLNLDDLATLEAYAQQADTDQLLDLLREIEMTEQRATRHLNLHMALEMILLRLRGAVT
                     ASPIPQPI"
     gene            1523..3556
                     /gene="metG"
                     /locus_tag="VLL06_02245"
     CDS             1523..3556
                     /gene="metG"
                     /locus_tag="VLL06_02245"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HSS29818.1"
                     /translation="MSSQNTFYITTPIYYVNDVPHIGHAYTTIAADVLARYWRLRGRD
                     VFFLTGLDEHGQKVQQAAAKAEIDPQAHCDKLAPQFQKLWERLNISNDAFIRTTDPPH
                     KFVVQRYLQDLHDKNQIYQDSYTGWYCTFDERFWTEKDVTDGLCPDCKRPVEQLSEHN
                     YFFKMGQYQDRLIEHIKQHPNFIRPESRRNEVLGFLTTQKLGDLSISRPKSRLSWGIE
                     LPFDKHYVTYVWFDALVNYVSALEYLPKKPALDQFWPANVHLVGKDILTTHAVYWSTM
                     LMALNLPLPETIFAHGWWTVDGEKMSKSRGNVVDPYAIAEKYGVYALRYFLLREIPFG
                     QDGDFSESALVRRVDSDIANGLGNLLRRTLTLIERNVGGSVPEVPPPLDTDVEKSLTV
                     AGLEAFSLADSHIEEMAFSRALEQIWQLVRAADQYLETHAPWTLAKDPDKRKLLERVL
                     YRAADSLRLLAIAVYPFMPSTAYAIRDQLGLNPDFFSNPIPIEERQWHRLRPGTRIHI
                     GDALSPRLSTYALTGHETKLSVSQGAKTVSDATTPPQPTTAIPAPITAALQTAAPVAV
                     APAQITIDEFMKIQLKTAKVLTVERVPKSEKLLKLQVSLGEGTEQRQIVAGIGKKYEP
                     EALIGKTIVIVANLKPAKLMGIESQGMVLAAGDSEVRGLVTILEEVDPGTKVK"
     gene            3562..4413
                     /locus_tag="VLL06_02250"
     CDS             3562..4413
                     /locus_tag="VLL06_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29819.1"
                     /translation="MSIRPRLLFLLLILLIPLPCLAIPAETVSPPNPHQNEPKPTTIL
                     VRVVAHGSMVLGKDMGGARITITDAASGKLLAAGLQQGDAGDQNQIMRTPRMMGEPIY
                     SSRPSAAFTTTVSLTHPILVEIAAEGPLAYPASSQRASTTVWLVPGQDMTSDGIVLHL
                     FGYIVQIEHPKSGDPLIAKDDVVLRASVRTLSGALVRPHGDWDSRKVQIYGEVLIGNR
                     VLERLQLFYNNDHATFEAPFFVPLPKDAPDGITLRVVAADPSSGNVGVGKAKYPVLNE
                     QLKPPTR"
     gene            4449..>5077
                     /locus_tag="VLL06_02255"
     CDS             4449..>5077
                     /locus_tag="VLL06_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSS29820.1"
                     /translation="MLSVTVFAAEEHQRPRQPNDIKQYLEQLDSEDRDQYQKPRQVIE
                     ALNLKPGLAVADLGSGSGYFTRRFIEAVTETGKVYAVDVEPEMLKYAEESVVHMHRSY
                     TAEFILARPDNPKLPYGSIDLLFLCNTYHHLEERTKYFSDTKSSLKPGGRIAVIDFYH
                     DERSGELGFPKQHLVPRETVIAELTKAGYTLIREHQFLPKQYFLEFAPSR"
BASE COUNT         1177 a         1505 c         1324 g         1071 t
ORIGIN      
        1 ctgctgatct tcgcggcgag atgtcagcac gtgacgcagc tcgtcatacc ggcgttgcgg
       61 caagataccg tggtgttatg cgaccgattt tccgattcca cctttgccta tcaaggattc
      121 gcacgtggcc ttgatctgca atggctgcgg aaagtcaacg aagtcgcaac aggtgggctg
      181 acgccggatc tcaccctcgt actcgacctc ccggtctcag taggtttggc cagacggcga
      241 gccgatattg gacaacagaa tcgactggac cgtgagacgg aacgcttcca tcggaaggta
      301 cgccggggat ttctggcgct tgcggccgaa gagccaaacc gcatgaccat cgtgaatgcg
      361 aaccggcccg cacaagaagt ccgggacgag ctcaccgaga tcgtgctagg ctggatggct
      421 cgaccgcgtc gttccacacg ccgccggagg tagcatgcca tttcgcgact gtatcggcca
      481 tcacacctcc atcgcgttct tacaatctgc cgtcacccat gaacggctgg ctcacgccta
      541 tctgttccac ggcgaggatg ccatcgggaa acgattgacg gccattcgtc ttgcccaagc
      601 gctcaactgc gaacggccgc ctgaagcaga cgacctcgac agttgtggtg catgccgatc
      661 ctgccgacag atcgaagccc tcacgcatcc ggactttttt gtcatcgaac cggatccgga
      721 acaggccact caacagatca agattgagca agtccgcgag atcgagcacc agatcatgta
      781 tcgtccactt atcggtgaac gaaaaatctg tctgatcgac aacgccgacc gcatgacgat
      841 cggtgcagcc aacgcgctgt tgaaaacgtt ggaagagccg cccgcgcata gtcttttcct
      901 cgtcatttca agtcgcccgg cagccctgcc tgcaaccatc cggtcgcgct gtcaggccct
      961 gcgctttacg actccggctc gtacacaggt cgaagccgcg cttatcttga agcgtgaact
     1021 ccctcccaca gaggcgcgat tcctcgccat aatcagcgag ggccgtatcg gggaggccct
     1081 taccgccgat gtgaaagata cgcgcgcgcg ccaacaggaa ctcgttgaat tggttcgccc
     1141 ccaatccttg cagtcgatcg ggtccatact gtcctcggca gaagcgatcg cgaaggccga
     1201 ccgtgcacag gacatgctcg gctggctggc tcgctggatc cgcgacctcg tcttgattca
     1261 ggtcggagga gaccgtgacc agttgctcaa cctcgatgat ctcgcaacac tggaagccta
     1321 tgcgcaacag gccgacacgg atcagctatt ggacctgctc cgtgagattg agatgaccga
     1381 gcagcgagcc acgcggcacc tcaatctcca catggcgctg gaaatgatcc tgctccgtct
     1441 gcgcggcgcc gtgaccgctt cacctatccc gcaacccatc tagctatcat cgtttattac
     1501 ctggtatctt ttctcacacc gcatgagtag ccagaacacc ttttacatca cgacgccaat
     1561 ctattacgtg aacgacgtgc cgcatatcgg acacgcctac accacgatcg ccgccgatgt
     1621 gcttgctcgc tactggcgcc tgcgaggtcg cgatgtcttc tttctcactg gcctcgatga
     1681 acacggacaa aaggtccagc aggcagcggc caaagccgag atcgacccgc aagcccactg
     1741 tgacaaattg gcaccccagt ttcagaagct ttgggaacgt ctgaacatct ccaatgatgc
     1801 gttcatcaga acgacagatc ctccgcataa attcgtcgtc caacgatacc ttcaagacct
     1861 ccacgataaa aaccagattt atcaggattc gtacacggga tggtactgca cgtttgatga
     1921 gcggttttgg acggagaagg atgtgactga cgggctctgt ccggactgta agcgtccagt
     1981 ggagcagctc agtgagcaca actatttctt caagatgggc cagtaccagg atcgcctgat
     2041 cgagcacatc aagcaacacc cgaacttcat ccgtcctgag tcacgccgta atgaagtcct
     2101 gggattcttg accacgcaga agctcggcga cctttcgatc tcacgaccaa aatctagact
     2161 ctcctggggc atcgagttgc cctttgacaa gcactacgtc acatatgtgt ggttcgacgc
     2221 cctcgtgaat tatgtctctg ccctcgaata cctgcccaaa aaaccggcgc tcgatcagtt
     2281 ctggcctgcg aatgtccacc tggtcggtaa agacattctc accacccacg ccgtctattg
     2341 gtccaccatg ttaatggccc tcaatctacc gttaccagaa acgatttttg cgcatggttg
     2401 gtggacggtg gacggcgaga aaatgtcgaa gagccgcggc aacgtggtcg atccctatgc
     2461 gatagcggaa aagtacggcg tctacgcgct tcgttacttt ctgctgcgcg aaataccttt
     2521 cgggcaggat ggtgatttct cggaatcggc attagttcgg cgcgtcgata gtgacatcgc
     2581 gaatggtcta gggaacttgt tgagaaggac acttactcta attgagcgta atgttggagg
     2641 atcagtcccg gaggttcccc ccccgcttga taccgacgtc gagaaaagtc ttacggtcgc
     2701 aggcctggag gcattcagcc tggcggattc gcacatagag gagatggcgt ttagtcgagc
     2761 tctcgaacaa atttggcaac tagtacgcgc tgccgatcaa taccttgaaa cccatgctcc
     2821 ttggacctta gccaaagacc cggacaagag aaagctgttg gaaagagttt tatatagggc
     2881 cgcggactca ctccgcctct tagcgattgc cgtatatccg ttcatgccca gtacagctta
     2941 tgcgattagg gatcaattgg ggttgaatcc ggattttttt tcaaatccca ttccaataga
     3001 agaaaggcag tggcatcgtc ttcgtccagg caccaggatt catataggag atgcattatc
     3061 tcccagactg agtacatatg cgctgactgg gcatgaaaca aaactatctg tatcacaagg
     3121 agccaaaaca gtgagcgacg caacgactcc cccgcaacca acaacagcga ttccagcacc
     3181 aatcactgct gcgctacaga ctgcagcacc agtggctgtg gccccggcgc agatcaccat
     3241 cgacgagttc atgaagattc agctgaagac tgcaaaggtc ctcacagtcg aacgcgtccc
     3301 caagtcggag aaattgctca agctgcaagt cagtcttgga gaaggcacgg aacaacgtca
     3361 gatcgtcgcc ggcatcggca agaaatatga gccggaggcc ttgatcggca agacgattgt
     3421 catcgtcgcg aatctgaaac cggcgaagct catgggcatc gaatcgcaag gtatggtcct
     3481 ggccgcaggt gatagtgagg ttcgtggcct tgtgactatc cttgaagaag tcgatcccgg
     3541 caccaaagtg aaatgaatca cgtgagcatc aggccccgcc tgcttttcct cctcttgatc
     3601 ctcctgattc ccctgccctg cctcgccatt ccggctgaaa cagtttcacc gccgaacccg
     3661 caccagaacg agccgaagcc cacgaccatt ctcgttcgag tcgtggcgca tggatcgatg
     3721 gtgctgggga aggacatggg cggggctcgc attaccatta cggatgccgc cagcgggaag
     3781 cttctcgccg ccggcctgca acagggtgac gcgggggatc agaatcagat catgcgtaca
     3841 ccccgcatga tgggagagcc gatatatagt tctcgcccct ccgccgcctt cacgaccacc
     3901 gtatcgttga cccatccgat tctcgtggag attgcggcag aaggaccact tgcctaccct
     3961 gcatcgtccc agcgcgcctc gacgactgtg tggctcgttc ccggacagga catgaccagc
     4021 gacggcattg tccttcatct ctttggctac atcgtccaga tcgaacatcc gaaatcaggt
     4081 gacccgttga tcgcgaaaga cgatgtggtc ctgcgtgcat cagttcgaac attgtccgga
     4141 gcgctggttc gcccgcatgg ggattgggat tctcgaaagg tccagatcta tggcgaagtt
     4201 ctgatcggca accgcgtact tgagcggttg cagctgttct acaataacga ccacgccact
     4261 ttcgaagcac cgtttttcgt tcccctcccg aaggacgcgc cggacggaat caccctccgc
     4321 gtcgtggcgg ccgacccctc cagcgggaac gttggtgtgg ggaaggcgaa atatcccgtg
     4381 ctcaacgaac aactcaagcc accaacacga tgatgcgacg tctactgtcc cttactatga
     4441 cctgctgctt gctgtcagta acagtctttg ccgcagagga acatcagcgt cctcgccaac
     4501 cgaacgacat caagcaatat ctggagcaac tcgatagcga ggaccgagac cagtaccaaa
     4561 aacccaggca ggtcatcgaa gccctcaatc tgaagccggg cctggccgtg gccgacctcg
     4621 gctcagggtc cggctacttc actcggcgat tcattgaagc ggtgacagag acaggcaaag
     4681 tctatgcggt cgatgtggaa ccggagatgt tgaagtatgc cgaagagagc gtcgtccaca
     4741 tgcatcgatc ctatacggct gaatttatcc tggcgcgacc tgacaaccca aaacttccct
     4801 atggatcaat cgatctcctc ttcctgtgca acacctatca tcaccttgaa gaacgcacaa
     4861 aatacttctc cgataccaag tcatcgctga agcccggtgg tcgcatcgcc gtcatcgact
     4921 tctaccacga cgaacgctcg ggtgagctcg gttttccgaa gcagcatctg gtccctcggg
     4981 aaacggtgat tgcagaactg accaaggctg gatataccct cattcgtgaa catcaattct
     5041 tgccgaagca gtactttctc gaattcgctc cctcacg
//
LOCUS       DATIPF010000090         3254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1165876, whole genome shotgun sequence.
ACCESSION   DATIPF010000090 DATIPF010000000
VERSION     DATIPF010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165876"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VLL06_02260"
     CDS             complement(<1..400)
                     /locus_tag="VLL06_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSS29821.1"
                     /translation="MNYCSACGAPVTKKVPVGDNLPRFVCDTCLAIHYHNPKIVAGCI
                     PEWDGYILLCRRAIEPRSGLWTFPAGFMEIGESTEQAAARETFEEAQAQVDRISLFAV
                     LSLPHIGQTYLVFRGPMLSPDFGAGEESLEV"
     gene            477..1652
                     /locus_tag="VLL06_02265"
     CDS             477..1652
                     /locus_tag="VLL06_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HSS29822.1"
                     /translation="MDSTKRRFFAPCPRGLEGVLEAELHGLGVPTTTKMDGGIGFQAP
                     WSTMYWVNLKSHIASRVLWEVGQSPYRSEDDAYHAAYALAWPDWFAPSQTIKVKVSAR
                     RCPLPSLDFLTLRIKDAICDKFVATRRKRPSVNTEDPNIRLDAFLDQHTVTFYLDTSG
                     EPLFKRGHRMVSVEAPLRENLAAGILRLAGWTPKEILLDPMCGGGTIPLEATLMARQI
                     PPGISRNFGFERLLVHDTQRWGHLREAARTKQLAEVPAAVYASDHDPTMVKIAQRTFQ
                     GAGVAIDIRLKQSDILELDAPADQGVLVINPPYGVRLSQPDELESFYPKLGDWLKQRF
                     TGWRAYVLTGDARVPKLIGLAPSKRIPLFNGPLECRLYEFLIVQGGARRRLVPPTQA"
     gene            2117..>3254
                     /gene="hpnR"
                     /locus_tag="VLL06_02270"
     CDS             2117..>3254
                     /gene="hpnR"
                     /locus_tag="VLL06_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008169 - C-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid C-3 methylase HpnR"
                     /protein_id="HSS29823.1"
                     /translation="MRFLAVHPGPLMYTKIYLRLEPLGLEMVAQAARQAGHDVRLIDL
                     QVETWKDYLALIRTWRPDAVAFGCNYLANVPEIVDLAKFTKKEIPQVFIFVGGHSASF
                     VAREFLEHGNGAIDCVLKGEGEVSVHKLLLAVEHDRGAITSVPGVVALDGEGPAPQFI
                     ENLDDVRPARDLVRHRHKYFIGVLDPCASIEFARGCPWDCSFCSAWTFYGRSYRTVST
                     EKAVEELERVQEPAVFLVDDVAFIQAKQGFEIGEAVARRGIKKKYHMETRGDVLLRNK
                     EVFKFWKTLGLHYLFLGVEAIDEEGLRMHRKRISLGKNFEALECARSLGISVAINLIA
                     DPDWDRKRFETVRQWCLDIPEIVTISVNTPYPGTESWHTTSAKIQ"
BASE COUNT          749 a          961 c          892 g          652 t
ORIGIN      
        1 ggacctcaag gctttcctca cccgccccga aatctgggga cagcatcggt cctcgaaaca
       61 ccaggtaagt ctggccgata tgcgggagac tcaggaccgc aaagaggctg atgcgatcga
      121 cctgagcctg tgcttcttca aatgtttcgc gagcggcagc ctgctccgtg ctctcgccga
      181 tttccataaa gccggcagga aaggtccaga gacctgacct aggctcaatc gcacgccggc
      241 agagaagaat gtatccgtcc cactccggaa tacaacctgc aacgatcttt ggattatggt
      301 aatgaatggc cagacacgta tcgcagacaa accgaggcag gttgtcgccg acgggaactt
      361 tcttcgtaac tggagcaccg caggcgctgc aatagttcat ggtactacga tgatgggtga
      421 cgagtgaccc ggggaaagaa cgtatcgtgt cgcgcgagtc tcgtccgatt atgagcatgg
      481 atagcacaaa acgacggttc tttgcaccct gcccgcgcgg actcgaaggt gtgctcgaag
      541 cagagctaca tggcctcggc gtgccaacga cgacaaagat ggacgggggt atcggcttcc
      601 aggccccctg gtcaaccatg tactgggtga acctgaagtc tcacatcgcg agccgcgtcc
      661 tctgggaagt tggacagagc ccctatcgtt cagaagacga tgcctatcac gcggcctacg
      721 cgctcgcctg gcccgattgg tttgcgccgt cgcagaccat caaggtcaag gtcagcgcgc
      781 gcaggtgtcc tctccccagc ctggactttc tcaccctccg gatcaaggac gctatctgcg
      841 ataaatttgt cgccacgcgc cgcaaaagac ccagtgtcaa tacagaggac ccgaacatcc
      901 gactcgacgc gtttctcgac cagcacaccg ttacgttcta tctggatacc tctggcgaac
      961 ctctcttcaa acgtggccat cgcatggtct ctgtcgaagc cccgctgaga gaaaatctag
     1021 ctgcagggat tcttcgactg gccggctgga cccccaagga aatcctactc gaccccatgt
     1081 gcgggggagg gaccatcccg ctcgaagcca cattgatggc ccgccagatc ccgccgggta
     1141 tctcacggaa tttcgggttc gaacggctgc ttgtccacga tacccaacgc tggggccacc
     1201 tgcgcgaagc cgcacgtacc aaacaattgg ccgaggtacc agccgccgtc tatgcctccg
     1261 accatgatcc cacgatggtg aagatcgcgc agcgcacgtt tcaaggcgca ggagtcgcca
     1321 tcgacatccg tttgaagcag agcgatattc tcgaactcga cgcgccagcc gaccagggcg
     1381 tcttggtgat caaccctccc tatggagtcc gcctcagcca accggacgaa ctcgagtcgt
     1441 tctaccccaa gcttggcgat tggctgaagc agcgattcac cggatggcgc gcttacgtgc
     1501 tgacgggcga cgctcgcgta ccaaaattga tcggcctcgc cccgtccaaa cgcattcccc
     1561 tcttcaacgg ccccttggaa tgccgcctct atgaattcct catcgtccaa ggtggagcca
     1621 gacgccgact cgttccgccg acgcaggcct agcacgacct gcccatccca ttgagtcgac
     1681 accgaacggc agtgagccat cgcctatcgc caatttctca tcacaccctc ttaagtctcg
     1741 taaatccctt gtaaaaaccc cttacggttt acgtcccaaa gcttcgagca tcggttccac
     1801 ctctgctgat aattagtagg atcgctcttc aaaactaggg ttcaccctag cgctatgcgt
     1861 tctctttgaa gcctattgtg tcctagctca tgaagcccaa aacatacaag caaaagacct
     1921 cactctatgg ctcctggccc gacatgaggc aaataagaga tgaaaatcct cgctaccttg
     1981 atcgtagggg aaaacgtcgc ccgcgtggcg gttgccggtc agcaaaggcc aggatgggtg
     2041 gaccagcagc attcatgaaa cagtatcgac ggcccagaat cagagcccac atctttcaga
     2101 gaggagagtt gaggaaatga gattcctcgc agttcacccc ggtcctctga tgtatacgaa
     2161 gatctatctt cgcttggaac cactcggact cgagatggta gcccaggccg cccgccaagc
     2221 agggcatgac gttcgcctga tcgacctgca agtagaaacg tggaaagact acctggcgct
     2281 catcagaact tggaggcctg acgccgtggc attcgggtgc aactacctgg cgaacgtgcc
     2341 ggagattgtg gatctcgcca agttcaccaa gaaggaaata ccacaggtct ttatttttgt
     2401 ggggggacat agtgcctcct ttgtggcgcg agagtttttg gaacatggga atggcgccat
     2461 agattgcgta ctcaagggcg aaggagaagt ctccgtccac aagctgttgc tggctgtgga
     2521 gcatgaccga ggggccatca cgtcagtccc aggagtcgtg gccttggacg gtgagggacc
     2581 tgcgccgcaa ttcatcgaga acctcgacga tgtacgtcct gcgcgcgatc ttgtccggca
     2641 ccgtcacaag tatttcatcg gagtcctgga cccctgcgca tcaatcgagt ttgcgcgagg
     2701 ctgcccctgg gattgctcct tctgcagcgc ctggaccttc tacggtcgca gttaccgaac
     2761 ggtcagcacg gagaaggccg tcgaagaact ggaacgggtc caagaaccgg cagtcttcct
     2821 ggtcgacgac gtcgcattca tccaggcgaa gcaggggttc gaaatcggtg aagcggtcgc
     2881 acggcgaggg atcaagaaga agtaccacat ggaaacgcgc ggcgatgtct tgttgcgcaa
     2941 caaggaggtg ttcaaattct ggaaaacgtt gggactccat tacctgtttt tgggggtgga
     3001 ggcgatcgat gaggaaggac tccggatgca tcgcaagcgt atctcactgg gcaaaaactt
     3061 cgaagcactg gaatgtgccc gctcccttgg catcagcgtg gccatcaatt tgatcgccga
     3121 tcccgactgg gatcgaaagc gattcgagac agttcggcaa tggtgcctgg acatccctga
     3181 aatcgtgacc atcagcgtca atactcctta cccaggaacc gagagctggc acacaacatc
     3241 ggccaagatc caga
//
LOCUS       DATIPF010000091         7231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_900265, whole genome shotgun sequence.
ACCESSION   DATIPF010000091 DATIPF010000000
VERSION     DATIPF010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900265"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3865
                     /gene="rpoC"
                     /locus_tag="VLL06_02275"
     CDS             <1..3865
                     /gene="rpoC"
                     /locus_tag="VLL06_02275"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HSS29824.1"
                     /translation="LAAPVAHIWFLKGVPSRIGTLLDMSLKGLERILYFESYVCVDPG
                     STDLTEKELVSEEKLRELQAAGYSPGSYKVGIGADAIRELLRKIDINAKWDEIKAKAK
                     TSASAALKKKYAKQLKVIEAFRKSENMPEWMIMDVIPGLPPELRPLVPLDGGRFATSD
                     LNDLYRRVINRNNRLKRLIELKAPGVIIRNEMRMLQEAVDALFDNGRRGRAIRGPNKR
                     PLKSLSDMLKGKQGRFRQNLLGKRVDYSGRTVIVVGPELRLHQCGLPKKMALELFKPF
                     IFHKLEERGAATTIKSAKRLVEKERPEVWDVLDEVIREHPVLLNRAPTLHRLGIQAFD
                     PVLVEGKAIRLHPLVCAAFNADFDGDQMAVHVPLSVEAQVEARVLLMSINNILSPANG
                     KPIAVPSQDMVLGCYWLTKERAGCKGEGKVFGSPEEVRIAFDSQELVEHARIKVRIEG
                     NLVQTTVGRVILSEVLPPGMPFATANKLMTKKEMTKLIDTVYRQCGHRETVTFLDKVK
                     DTGFQYATRAGISICIDNMHIPTKKQELLGKAQHEVTEIERQYAEGLITNGERYNKVI
                     DIWAHVTEQIANEMMKELGAGGDPTKAESFNPIFMMADSGARGSSQQIRQLGGMRGLM
                     AKPSGEIIETPITANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTRRLVDIAQ
                     DVIINEIDCGTTDGIIVSALVEGGEIIQPIEERVLGRLAAEDIRDPVTGEIIVSFNEE
                     IDEDRTKAIVEAAVDHVKIRSVLTCQSRRGVCRSCYGRDLARGRLVEKGEPVGVIAAQ
                     SIGEPGTQLTMRTFHIGGTASKVVEQTVTESKHAGTIKFMSFDAKKNADFPNAGIAVQ
                     NKEGEWVVMNRNAKIAIVDETGREREKYGVVYGAKIKVKNGGRVEVGQKLVEWDPYSL
                     TILTEVGGKVAYGDIIEGVSMKEEFDEVTGLSRKVIVEHSGATLRPRVSIKDEGGKTA
                     KVAAAANVARYLLPVGAHIFVEKGAVVTPGDVLAKIPRETTKTKDITGGLPRVAELFE
                     ARKPKETAVISEIDGEVSYDGFVKGMRKVLVNNKMGDVKEYFIPKGKHVNVHEGDWVR
                     AGEPLMDGSANPHDILDVLGPNELQKYLVDEVQDVYRLQGVTINDKHIEIIVRQMLRK
                     VRIEDPGDTEFLPGSQVSKMLFDTENERVLAKGGQPGLGKPVLLGITKAALTTDSFIS
                     AASFQETTRVLTEAAINGRIDNLLGLKENVIVGRLIPAGTGFEEYRDTFVISPKPEPV
                     IAGQGDVAAVAPEGVATGLGEPARS"
     gene            3985..4356
                     /gene="rpsL"
                     /locus_tag="VLL06_02280"
     CDS             3985..4356
                     /gene="rpsL"
                     /locus_tag="VLL06_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HSS29825.1"
                     /translation="MPTINQLVRKGRRLAHAKTKSPALKSCPQKRGVCLRVYTSTPKK
                     PNSALRKVARVRLTNGMEVTTYIPGVGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGAL
                     DAVGVADRKQSRSKYGAKRPK"
     gene            4396..4866
                     /gene="rpsG"
                     /locus_tag="VLL06_02285"
     CDS             4396..4866
                     /gene="rpsG"
                     /locus_tag="VLL06_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HSS29826.1"
                     /translation="MPRTRFLDHRDPLPDVRYRDKLVGKFLNILMSGGKKSTAERICY
                     GAFDVIHEKTGNDPLKVFRTAIDNVKPVVEVKSRRVGGASYQVPVEIRPARRMSLALR
                     WLAEYSRTRGGKSMREKLAAELLDASNNTGAAVKKREDVHRMAEANKAFAHYRW"
     gene            4977..7058
                     /gene="fusA"
                     /locus_tag="VLL06_02290"
     CDS             4977..7058
                     /gene="fusA"
                     /locus_tag="VLL06_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HSS29827.1"
                     /translation="MARQSTLEQTRNIGIMAHIDAGKTTTTERILYYTGISHKMGEVH
                     EGAATMDWMEQERERGITITAAATTCFWRDHRINIIDTPGHVDFTIEVERSLRVLDGA
                     VAVFDSVQGVEPQSETVWRQADKYSVPRIAFMNKMDRIGADFYTSVQTMIDRLGANPV
                     PIQIPIGRESEFRGSIDLVTMKAYVYDDETLGAKYIIDEIPADLVARAKEYRDKMLDA
                     VAEFDDQAMEKYLNGQPLTEQEIRRAIRAGVLSMKMTPILCGSAFKNKGVQQLLDGVV
                     DFLPSPLDVESVTGIDPNTEQEVKRRPSDSEPFAALAFKIMTDPFAGQLTFLRVYSGT
                     LKTGTSVSNVTKGTKDRVGRLLKMHANKREDIDVAYAGDIVAAVGLKGATTGDTLADE
                     KQPVLLEVMKFPEPVIAMAVEPKTKQDQEKMGFALQKLAQEDPSFRVKTDEETAQTII
                     AGMGELHLEIIVDRMLREFKVEANVGKPEVAFRETIRKKAEAESKYIKQTGGRGQYGH
                     VVLTVEPAESGKGLDFINKTVGGSIPREFIPAIEKGVKERLDSGVIAGYPIRDVRVTV
                     IDGSFHDVDSNEMAFKIAGSMAFLDACKRADPVLLEPIMKVEVVVPQDFMGDVIGNLN
                     GRRGKIQGMKSRAAAQVIDALVPLSEMFGYATDLRSRTQGRATYSMEFDRYEPVPRQI
                     SEAIIAKYRGE"
     gene            7097..>7231
                     /locus_tag="VLL06_02295"
     CDS             7097..>7231
                     /locus_tag="VLL06_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSS29828.1"
                     /translation="MAKAKYERRKPHVNIGTIGHVDHGKTTLTAALTKVSAEKGMAKF
                     I"
BASE COUNT         1698 a         1685 c         2300 g         1548 t
ORIGIN      
        1 gctcgcggca ccggttgcgc atatttggtt tctcaagggc gtgccgagcc ggattggaac
       61 cttgctcgac atgagcctca aagggttgga gcggattctc tatttcgaga gctatgtgtg
      121 tgtggatcca ggatcgacag atttgacgga gaaggaacta gtttcggagg aaaagcttcg
      181 cgaactccaa gctgcgggat acagtcccgg ttcctacaaa gtcggcatcg gtgccgatgc
      241 cattcgggag ttgctccgta agatcgatat taatgccaag tgggatgaga ttaaggcgaa
      301 ggccaagacg tcggcttccg cagcgctgaa gaagaaatat gcaaagcaat taaaagtcat
      361 cgaggccttc cgcaagtccg aaaacatgcc tgaatggatg attatggatg tgattccggg
      421 cctgccaccg gaattgcgcc cacttgtgcc actggacggc ggacgttttg cgacatcgga
      481 tttgaacgat ttgtaccgcc gcgttatcaa tcggaacaac cgtctgaagc ggttgatcga
      541 gctgaaggcg ccgggcgtga ttatccgaaa cgaaatgcgg atgttgcagg aagcggttga
      601 tgcgctcttc gataatggtc gtcgcgggcg tgcgattcgt ggacccaaca agcggccgct
      661 caaatcgttg agcgatatgc tgaaggggaa gcaggggcgc tttcgccaaa acctgctcgg
      721 caaacgggtc gattattccg gccgaaccgt cattgtcgtg ggacctgagc ttcgattgca
      781 tcaatgcggg ttacccaaga aaatggcgct cgaactcttc aagcccttta tcttccacaa
      841 gctggaagag cgtggtgcgg caacgacgat caagagtgcc aaacgcttgg tcgaaaaaga
      901 acggccggaa gtgtgggacg tacttgacga ggtgattcgg gaacatcctg tgctgctgaa
      961 ccgtgcaccg acgttgcacc gtcttggtat ccaggcgttt gatcctgtgt tggttgaagg
     1021 caaagccatc cggctgcatc cgctggtctg cgcggcattc aatgcggact tcgacggtga
     1081 ccagatggcc gttcacgttc ccctgtcggt cgaagcgcag gttgaggcac gcgtgctgct
     1141 aatgtcgatc aataacattc tctcgcctgc caacggcaag ccgattgcgg tgccgtcgca
     1201 ggacatggtg ctcggctgtt attggctgac caaggagcgt gcaggatgca agggagaggg
     1261 gaaagtattc ggctcgcctg aggaagtgcg tattgcattc gattcgcagg agttggtgga
     1321 gcatgcgcgg atcaaggtgc gcatcgaggg aaatctcgtt cagacgaccg tcggacgagt
     1381 gatcctgtcg gaagtgcttc ctccgggaat gccctttgcc acggcgaata aactcatgac
     1441 caagaaggaa atgacaaagc tcatcgacac cgtctatcgt cagtgcggtc accgtgagac
     1501 cgtcacgttc ctcgataagg tcaaggacac gggattccag tatgccacgc gggccggcat
     1561 ctcgatttgc atcgataaca tgcatattcc gaccaagaag caagaattgc tggggaaggc
     1621 tcagcatgag gtgacggaaa tcgagcggca gtatgccgag gggttgatca ccaacggcga
     1681 acggtataac aaggtgatcg acatctgggc ccatgtaact gagcagattg ccaatgaaat
     1741 gatgaaggag ttgggtgcgg gtggcgatcc taccaaggct gagtccttca acccgatctt
     1801 catgatggcg gattccggtg ctcgcgggag ttcccagcag atccgccagt tgggtggtat
     1861 gcgcggattg atggcgaagc cgtccggtga aattatcgaa actccgatta ccgccaactt
     1921 ccgcgaagga ttgacggtgt tgcagtactt catttcgacg cacggtgccc gtaaggggtt
     1981 ggcggacacg gcgttgaaga cggccaattc cggctatctc acgcgtcgct tggtggatat
     2041 tgcacaggat gtgattatca acgaaatcga ttgcggtacc accgacggca tcattgtgag
     2101 cgcattggtc gagggcggcg aaatcattca gccgatcgaa gagcgggtgt tgggacggtt
     2161 ggccgcggaa gatattcgtg acccagtgac aggtgagatc atcgtgtcgt tcaatgagga
     2221 gatcgacgaa gaccggacaa aggcgatcgt ggaagctgcg gtggatcacg tcaagattcg
     2281 ttcggtgctg acctgtcagt cacgccgtgg agtctgtcgg tcgtgttatg ggcgcgattt
     2341 ggcccgcgga cggttggtgg aaaaaggcga acccgttggt gtcatcgcgg cacagtcgat
     2401 cggtgagccg ggaacacagc tgaccatgcg gacgttccac attggtggaa cggcgagcaa
     2461 agtggttgag cagacggtga ccgaatccaa gcacgcaggc acgatcaagt tcatgagctt
     2521 cgatgccaag aagaatgccg actttcccaa cgccgggatt gcggtccaga ataaagaggg
     2581 cgaatgggtc gtcatgaatc ggaacgccaa gattgccatt gtcgatgaga caggacgtga
     2641 gcgcgagaag tatggtgtcg tctatggtgc caagatcaag gtgaaaaatg ggggtcgtgt
     2701 cgaggtgggc cagaaattgg tcgaatggga tccgtactca ctgactattc ttactgaggt
     2761 gggcggtaag gtggcgtatg gagacatcat cgaaggtgtg tccatgaagg aagagttcga
     2821 tgaagtcacc ggcctctccc gcaaggtcat cgttgagcat agcggcgcga cgttgcgccc
     2881 acgcgtgtcg atcaaggacg agggcggaaa gactgccaag gtggcagcgg cggccaacgt
     2941 ggctcgctat ctgctccctg tcggggcgca catcttcgtc gaaaagggcg cagtggtcac
     3001 tcccggtgat gtgttggcta agatccctcg ggaaacaact aagaccaaag atattaccgg
     3061 tggtctgcct cgcgtggctg agttgttcga ggcacgaaag ccgaaagaaa ccgccgtcat
     3121 cagcgagatc gatggcgaag tgtcgtacga tggctttgtg aaggggatgc gcaaagtgtt
     3181 ggtcaataac aagatgggcg atgtgaagga gtacttcata cccaagggca agcacgtcaa
     3241 cgtgcatgag ggcgattggg tcagggccgg tgagccgttg atggatggat cggccaatcc
     3301 gcacgacatt ctcgatgtac tgggcccgaa cgagttgcaa aaatatcttg tcgatgaagt
     3361 gcaggatgtc taccggctac aaggtgtgac gatcaacgat aagcacatcg agatcattgt
     3421 gcggcagatg ctgcgcaagg tccgaatcga agaccctggt gatacggaat tcttgccagg
     3481 cagtcaggta agcaagatgc tatttgacac ggagaatgaa cgagtgttgg cgaagggcgg
     3541 gcagccgggt ttagggaagc cggtcctact gggcatcacc aaggccgcat tgacgacgga
     3601 tagctttatt tcggcagcct cattccagga gaccacgcgt gtcttgacgg aggccgcgat
     3661 caacgggcgc attgataatc tgctgggctt gaaagaaaat gtgatcgtcg gtcgtctgat
     3721 tccggctgga accggctttg aagagtatcg cgacaccttt gtcatcagtc ccaaacctga
     3781 gcctgtgatt gccggtcagg gagatgttgc ggcggtcgct cccgagggtg tcgcgacggg
     3841 attgggtgag cctgctcgct cataatgcat ggggtgccgg agaaggcttg acactgcgat
     3901 caatgctcac tacaatcgac cggctttgcc cttgatgggt tgagtgacgc ggtcaaactg
     3961 tgccgaaatg agagtgacgt acgaatgcca acgattaatc agttagtcag aaaaggtcgc
     4021 aggctggcac acgcgaagac caagagccct gcgctcaaat cctgcccgca aaagcgtggc
     4081 gtgtgtcttc gtgtctatac ctcaacgcca aaaaaaccga actcggcgct gcgtaaagtt
     4141 gcgcgtgtcc ggttgacgaa tggaatggaa gtcacgacct acatccctgg tgtcggccac
     4201 aaccttcaag aacattccat tgtgctcgtg cgtggtggcc gcgtgaagga cttaccgggc
     4261 gtgcgctatc acatcgtcag aggtgccctc gatgctgttg gtgtggcgga ccgaaagcag
     4321 agccgttcga agtatggagc gaagcgtccc aagtagaaga ttgccaacca ttggtaccga
     4381 gagagtgaat gacacatgcc aagaactagg tttttagacc atcgagatcc tctccctgat
     4441 gtgcgatatc gggataagct tgttgggaag tttttaaaca tcctcatgtc gggtggaaag
     4501 aagagtacgg ccgagcgcat ttgctacgga gcctttgatg tcattcatga gaagacgggc
     4561 aatgatccgc tcaaagtatt tcggacggcg attgataatg tgaagccggt cgtcgaggtg
     4621 aagtcccgtc gggtaggcgg tgcctcatat caggtgccgg tagaaattcg tccggctcgt
     4681 cggatgtcgc tggcactccg atggttggcg gagtattcac gtactcgggg cgggaagagc
     4741 atgcgagaga agctcgcggc tgagttgttg gacgcgtcga acaatacggg ggcggcggtt
     4801 aagaagcggg aagacgtgca tcggatggcg gaggccaata aggccttcgc gcactatcgc
     4861 tggtagcgtc attctgtgct gcagctgggc cgtgctgcga tccgctcacg caggtgcgtg
     4921 taagtcgcag cgtaacaccg gccaggttgc ggcatatata tttttagggg aagtacgtgg
     4981 cacggcaatc aacattagag caaactcgga acatcggcat catggcccac attgatgccg
     5041 ggaagacgac gaccacggag cgcattcttt actacaccgg gatctcacac aagatgggtg
     5101 aggttcacga aggcgctgcg acgatggatt ggatggagca ggagcgggag cgcggcatta
     5161 cgattacggc tgctgcgacg acctgtttct ggcgagacca tcgtattaat atcatcgata
     5221 ccccgggaca tgtcgatttt acgattgagg ttgagcggtc gcttcgggtg ctcgacggtg
     5281 ctgtggcagt gtttgactcc gtgcaaggag ttgagccgca atctgagacg gtctggcggc
     5341 aagcggataa atacagtgtg ccgagaatcg cgttcatgaa caagatggac cgaatcggcg
     5401 cagattttta taccagcgtc cagacgatga tcgatcggtt gggagcgaat cctgttccga
     5461 ttcagatccc tatcggccgc gagtctgagt tcagagggtc gatcgatctg gtgaccatga
     5521 aggcctatgt gtatgacgat gagacgcttg gggccaaata tatcatcgac gaaatccctg
     5581 ccgatctggt cgcccgagcc aaggaatatc gcgataaaat gctggatgcg gtcgctgaat
     5641 ttgatgatca ggccatggaa aagtatctca atggtcagcc cttaacggaa caagagatcc
     5701 gccgagccat tcgggctggc gtgctttcga tgaagatgac tcctatcctg tgcggatcgg
     5761 cgttcaagaa caagggtgtc cagcagttat tggatggggt tgtggatttc cttccgtccc
     5821 cgcttgatgt cgaatcggtg actggaattg acccgaatac cgaacaagaa gtgaagcgac
     5881 gtccctcgga tagtgagccg tttgcggcct tagccttcaa gatcatgacc gatccatttg
     5941 cgggccagct caccttttta cgagtatact cgggcacgct gaaaacgggc acgtctgtct
     6001 cgaatgtgac gaaggggacg aaggaccgtg tcggacgtct cctcaagatg catgcgaaca
     6061 agcgggaaga cattgatgtg gcctatgcgg gtgatattgt ggcggccgtc ggactcaaag
     6121 gagcaacgac cggagacaca ctggcggatg aaaagcagcc ggttctactc gaggtcatga
     6181 agttccctga gccggtcatc gccatggcgg ttgagccaaa gaccaagcaa gatcaggaga
     6241 aaatggggtt cgcgcttcag aagttggcac aggaagaccc ctcgttccgg gtgaagacgg
     6301 atgaagagac ggcacagacc atcattgccg ggatgggtga gctgcatctg gaaatcatcg
     6361 tcgatcgcat gttgcgtgaa ttcaaagtgg aggccaatgt cggaaagccg gaagtggctt
     6421 ttcgggaaac gattcgtaag aaggctgagg ctgagtcgaa atacattaag cagaccggtg
     6481 gtcgtggcca gtatggacat gtcgtgctga cggtcgagcc ggcggaatca ggcaaaggtc
     6541 tggattttat taacaagacc gtgggcggct ctattccaag agagttcatc cctgcgatcg
     6601 aaaagggtgt gaaggaacgg cttgattccg gcgtgatcgc ggggtatccc attcgcgatg
     6661 ttcgtgtgac cgtgatcgat gggtcgtttc atgacgtcga ctccaatgaa atggcgttta
     6721 aaatcgccgg gtcgatggct tttctcgatg cctgtaagag agccgatcct gtgttgcttg
     6781 agccgatcat gaaggtggag gttgtggttc cccaggattt tatgggcgat gtgatcggca
     6841 atttgaatgg tcgccgtgga aagatccagg gaatgaaatc gcgggctgct gcgcaggtca
     6901 ttgatgcgct cgtgccgctc agtgaaatgt tcggatatgc gaccgatctt cggtctcgaa
     6961 cgcaaggtcg tgccacctat agtatggagt tcgatcgata tgagccggtg cctcggcaaa
     7021 tttcagaggc catcattgcc aagtatcggg gtgagtagtc gaattgattc ttgagtgagg
     7081 acggaaggga gcgggtatgg cgaaggcgaa atatgagcgg cggaagccgc acgtgaatat
     7141 tgggacgatt gggcacgtgg accacgggaa gacgacgttg acggcggcgc tgacgaaggt
     7201 gtcggcggag aaggggatgg ccaagtttat c
//
LOCUS       DATIPF010000092         3864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_811944, whole genome shotgun sequence.
ACCESSION   DATIPF010000092 DATIPF010000000
VERSION     DATIPF010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811944"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VLL06_02300"
     CDS             complement(<1..581)
                     /locus_tag="VLL06_02300"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HSS29829.1"
                     /translation="METAPRRKQGPTHHTVAVHTGCNTTIAAFLEFNGSRVRENGARL
                     LRMTERDESEERDRPHEVGTQSALACLTRDAFRIMEVEGGGIQNSPAGTLNHLRSKGF
                     SMAKIIPFHGTAYDTSIIGDVTQVVAPPYDIIDAAGQRALHDRHPQNIIRLELGLDCE
                     NGGGCMAPRTRRRTACGFSPSGTAHSLPARFLST"
     gene            complement(615..1619)
                     /locus_tag="VLL06_02305"
     CDS             complement(615..1619)
                     /locus_tag="VLL06_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HSS29830.1"
                     /translation="MPQSPITQSINTMADVWEAYRDELEGMERQARTNLDSSVALVNT
                     VAAHILNSGGKRIRPFLLLLSARLCGYTGRDHYQLASLVEFIHTASLLHDDVVDDADI
                     RRGRPTARKVWGNQISILVGDYLYSKAFCQVVDFRNHGINDVLGEACKKMAEGEVLQL
                     YYNGNPSMPEAEYLKIVEHKTAGLMAASCRMGAILADASDAQQDALFRFGLHLGIAFQ
                     VADDTLDYTANGEHLGKSLGQDLREGKATLPLLHLLHHCSDQDRQMIIDRMETRTHSE
                     EDLGRFIRLMVELGSITYAMDRARSYIAAAHQDLDQFEDSTAKRALSVTADYIVTRDR
                     "
     gene            1864..>3864
                     /locus_tag="VLL06_02310"
     CDS             1864..>3864
                     /locus_tag="VLL06_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HSS29831.1"
                     /translation="MSIPGLPAKQGLYDPAQEKDSCGIGFVVNIKGQKSHTIVQQGLQ
                     ILESLSHRGAQGCDACTGDGAGILLQVPHEFLKRAAGDVGVSLPNAGEYGVGMVFLPP
                     HPDARRQCEALFARIIGEEGARLLGWRDVPVKSNVIGVVAQSTEPYMRQVFIARDVLN
                     EGQFERKLYVIRKRVETAISESAIQGRHYFYISSLSAHTIVYKGLLLPEQMSAYYQDL
                     KDASLTSALALVHSRFSTNTFPTWPLAHPYRYICHNGEINTLKGNVNWMRARQGRLNS
                     DLFGQDLEKLFPVVSEQQSDSACLDNAVEFLTMGGRSLPHVMMMLIPEPWVANPQMDL
                     DRRGFYEYHAAMQEPWDGPAAVCFTDGRLIGATLDRNGLRPCRYQVTTDGLVILSSEA
                     GVLPMDARKIRQKGRLMPGRMFLVDTVQGRIIDDEEVKAEIVSRKPYRSWVTQYRISL
                     DELPDPSNVPQPDHPTIRQRQLAFSYTVEELKMVITPMVVEGQEATSSMGTDTPLAVL
                     SDRPQLLFKYFKQLFAQVTNPPIDPIREELVMSLTTSIGPKPNLMDEHPESCRRIRVK
                     QPILTNAELQKIREIADPHFKSKTLKMLFRVAEGPEGLGAAVDDLCRQASQAIRDGYK
                     FLILSDRGINEEWAPIPSLLGIASVHHHLVRECTRTEAGLIVE"
BASE COUNT          804 a         1066 c         1172 g          822 t
ORIGIN      
        1 gtagagagga agcgcgccgg taagctgtgc gccgtcccag acggtgagaa tccgcaggcc
       61 gtccggcgtc gagtacgcgg ggccatacag ccgccaccat tctcgcagtc gaggcccaac
      121 tccagacgaa tgatgttctg cggatggcga tcgtgcaagg cccgctgccc tgctgcatca
      181 atgatatcgt aaggtggtgc cacgacctga gtgacgtctc caataatcga ggtgtcatac
      241 gcggtgccgt gaaacggaat gatcttggcc atgctgaatc ctttgctccg taagtggttc
      301 agggtaccgg cgggactgtt ctggatcccg ccgccttcaa cttccatgat gcgaaatgcg
      361 tcacgcgtga gacaggcgag cgcagattgg gttcccacct catgtggccg gtctcgttcc
      421 tccgactcgt ctcgctcggt catccggagg agtcgagccc cgttctcccg gacacgcgag
      481 ccgttgaatt ccagaaacgc cgcaatagtt gtgttgcagc ctgtatgcac agcgactgtg
      541 tgatgggtgg gtccttgctt cctgcgtggt gccgtttcca cggggtattc cctggaaaca
      601 cgcggggaga aaggttatcg atcgcgggtc actatatagt cggcggtgac cgagagggct
      661 cgtttggccg tactgtcttc aaactggtcg agatcttgat gggctgcggc gatataggat
      721 cgtgcccgat ccatggcata ggtaattgaa cccagctcta ccatcaagcg aatgaaccga
      781 ccgaggtctt cttccgagtg ggtcctggtt tccatgcggt cgatgatcat ctgtcggtcc
      841 tggtcagaac aatggtgcag cagatgtaac agcggcagcg tggccttccc ttcgcggaga
      901 tcctgcccca gcgacttgcc caagtgttcg ccattggccg tatagtcaag agtatcgtcg
      961 gccacttgga aggcaatccc aagatggaga ccgaaccgga acagcgcatc ctgctgagcg
     1021 tccgaggcgt ctgcgagaat ggcgcccatg cggcacgagg cggccatcaa gccggccgtc
     1081 ttgtgttcga caatcttgag atactccgct tccggcatgg aggggttgcc gttgtaatag
     1141 agctgaagca cttcgccctc agccatcttc ttgcaagcct cgccgagcac gtcattaatc
     1201 ccatggttcc ggaagtcgac gacttggcag aaggctttgg agtagagata gtcgccgaca
     1261 aggatgctga tttggttgcc ccagactttt cgggcggtcg gtcggccccg gcggatatcg
     1321 gcgtcgtcga cgacatcatc gtggagcaga gatgcggtat gaataaactc aaccagactc
     1381 gcgagttggt aatgatcgcg accggtgtag ccgcagagac gggcagagag caggagtaaa
     1441 aatggtcgaa tgcgttttcc gccgctgttc aggatgtggg cggcgacagt attgacgagt
     1501 gccacactgg aatcgaggtt cgttcgagcc tggcgttcca tcccctccag ttcatcacgg
     1561 tacgcctccc agacgtccgc catggtattg atactctggg tgattggact ctggggcatg
     1621 cggcggatgg tagggcagag aaggaaacaa agtcaagcca atcacccctt tggggggcgg
     1681 cccggcatgg gcaatttgaa tgagtgacaa tcttgacaga agaatcatgc ttctattaag
     1741 gtgatcgaag cagatgtgaa cggaattcgc cttgtgtgtg acggggttat ccaccccgac
     1801 tgcctcttga tatagcctcc gtctggtcgc agacacgtct atatttttaa cgagataccg
     1861 accatgagta tccccgggct tccagcgaag caaggcctct acgaccctgc acaggagaaa
     1921 gattcctgtg ggatcggttt tgtcgtcaac atcaagggtc agaagtccca tacgatcgtt
     1981 cagcaagggc ttcagattct tgagagcctg agtcaccgag gggcccaggg ctgtgatgcc
     2041 tgtacgggag acggcgccgg cattctgctg caagtcccac acgagtttct caagcgtgca
     2101 gccggtgatg tgggtgtgtc tctgcccaat gccggagagt atggcgtcgg gatggtcttt
     2161 ctcccgcccc accccgatgc gcgacgacaa tgcgaagcgc tctttgcccg aattatcggc
     2221 gaggagggtg ctcggctcct tggttggcgc gatgtgccgg tcaagagcaa tgtgatcggc
     2281 gtcgtggcac aaagcaccga gccgtatatg cggcaagtgt tcattgcccg cgatgtcctc
     2341 aatgaagggc agtttgaacg caagctctat gtcattcgca aacgggtgga aacggccatc
     2401 agcgaatcgg ccattcaagg gcgtcactat ttctatatct cgagcctgtc cgcgcacacc
     2461 atcgtctata aggggctgtt gctcccggag cagatgtcgg cgtactacca ggacttgaaa
     2521 gatgcgagcc tgacgagcgc gctggccctc gtacattccc gcttcagtac gaatacgttt
     2581 cctacctggc cgttggccca tccctaccgg tacatttgcc ataacggtga gatcaatacg
     2641 ctcaagggca atgtgaattg gatgcgcgcc cggcaagggc ggctcaactc cgacctgttc
     2701 ggccaggatc tggagaaact ctttccggtc gtctccgagc agcagagcga ctctgcctgt
     2761 ctggacaatg ccgtggaatt cctgacgatg ggaggccgtt ctcttccgca cgtgatgatg
     2821 atgctgattc ccgaaccctg ggttgcgaat ccacaaatgg atctcgatcg gagggggttc
     2881 tacgaatatc acgcagcgat gcaagagccc tgggatggtc cggcagcagt ctgcttcacc
     2941 gacggcaggc tcatcggcgc gacgctcgac cgcaacggat tgcgcccctg ccgctatcaa
     3001 gtgacgacgg acggcctggt gattttgtcc tcggaagcgg gtgtgctgcc gatggatgcc
     3061 cggaagatca ggcagaaggg gcgcctcatg ccggggcgta tgttcctggt cgataccgtg
     3121 caggggcgca tcattgatga cgaggaagtc aaggcagaga ttgtgagccg caagccctac
     3181 cggtcctggg tcacccaata tcggatttcg ctcgatgaac tgcccgaccc cagcaatgtg
     3241 ccgcagcctg accatccgac gatccgccaa cgccagctgg ccttcagcta tacggtggaa
     3301 gagttgaaga tggtcatcac gcccatggtg gtggagggac aggaagcgac gtcgtcgatg
     3361 ggcacggata cgccgttggc cgtactgtcc gatcgaccgc aactgctctt caaatatttc
     3421 aagcagctgt tcgcccaggt gaccaatccg ccgatcgatc cgatccgcga agaactggtc
     3481 atgtcgctca cgaccagtat cgggcccaag ccgaatctga tggatgagca tccggagtct
     3541 tgccggcgca tccgggtcaa acagccaatt ttgacgaatg ccgaactcca gaagatccgc
     3601 gagatcgccg atccgcattt taagagcaag acactcaaga tgctgttcag agtggccgag
     3661 gggccggagg ggctcggggc ggcagtggac gatctgtgcc gccaggcgtc tcaggctatt
     3721 cgggatggct acaagttttt gatcctgagc gatcgcggta tcaacgaaga atgggcgccg
     3781 attccaagtc tcctcggcat tgcgtcggtt caccatcatc ttgtgcgcga gtgtacgagg
     3841 accgaagccg gtctcatcgt ggaa
//
LOCUS       DATIPF010000093         3140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_900339, whole genome shotgun sequence.
ACCESSION   DATIPF010000093 DATIPF010000000
VERSION     DATIPF010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900339"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="VLL06_02315"
     CDS             <1..1150
                     /locus_tag="VLL06_02315"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HSS29832.1"
                     /translation="PTLFPGTTMYKLEENYRSTQPILNLANCIIDEAAEKYTKRLFTR
                     KIDGPLPALVEAAGENTQSRFIAQKILELREEGIPLSEVAVLFRSSVHSFDLEIELSR
                     KGLPFIKRGGVKFIETSHVKDLLAHVRVIANPLDTVSWHRVLMLVEGVGPKKAQDLLA
                     TLVKSDKPFQTLREVTGRSSVGLKSLANTLESLSGAEDLRPANLVSQAYEYYLPILKE
                     QYDDYPKRIRDLDHLQTIAEGYPGVDEFLADLALEPPDGSAVGVDASDRDDERMVLST
                     IHSAKGLEWQCVFVIWVVDGRFPSVYSFVADDELEEERRLFYVAVTRAKRHLFLTYPI
                     NVFDKGSGMLLSKPSRFLDPVTPALLDQLVLVEEGGRPDWYARDEYSL"
     gene            1199..2092
                     /locus_tag="VLL06_02320"
     CDS             1199..2092
                     /locus_tag="VLL06_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29833.1"
                     /translation="MVPALCRVVAIGFLMGGLLLVPNVGKAEEPRPSTERMWRQLLNE
                     AHALGLPTKFLTAVPPSFVRFEFDELHSYAAEYHLGAHRMVLNRTLSFNGAGATLRPL
                     GRLTHAEIATLYHELFHAYLDYLVMAAGASSELVLDPVLAFARAQQACRYSAVLITPV
                     VQRSSETEERFLSEHESWEALNETWAVFVGWVVWNQLELTGRAGQSIQQSGKSQDEWV
                     RRLRSADREGMFRGYYEPEGPDERAVARKRYLAPASRLSEPEATVLMKDVLEFSPGLL
                     VRARGEFSDTDQVVMHHGACR"
     gene            2469..2554
                     /locus_tag="VLL06_02325"
     tRNA            2469..2554
                     /locus_tag="VLL06_02325"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2503..2505,aa:Tyr,seq:gta)
     gene            2639..2713
                     /locus_tag="VLL06_02330"
     tRNA            2639..2713
                     /locus_tag="VLL06_02330"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2671..2673,aa:Gly,seq:tcc)
     gene            2824..2899
                     /locus_tag="VLL06_02335"
     tRNA            2824..2899
                     /locus_tag="VLL06_02335"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2857..2859,aa:Thr,seq:ggt)
     gene            3006..>3140
                     /locus_tag="VLL06_02340"
     CDS             3006..>3140
                     /locus_tag="VLL06_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSS29834.1"
                     /translation="MAKAKYERRKPHVNIGTIGHVDHGKTTLTAALTKVSAEKGMAKF
                     I"
BASE COUNT          708 a          727 c          965 g          740 t
ORIGIN      
        1 cccgacgctc tttccgggca ccacgatgta taaactggag gaaaattatc gcagtacgca
       61 accgattctg aatttggcga actgtattat cgatgaggcg gcggagaaat ataccaaacg
      121 cctcttcacc agaaagatcg atggtccgtt accggctctg gtggaggcgg cgggtgaaaa
      181 tacacagtcc cggtttattg cgcagaagat tctcgagctt cgcgaggagg gcatacctct
      241 gagcgaggtg gcggtgctgt ttcgatccag tgtacattca tttgacctgg agatcgaact
      301 ctcgcggaaa gggttgccct ttatcaagcg cggaggcgtc aaatttatcg agacgtctca
      361 cgtgaaagat ctgcttgccc atgtgcgggt cattgcgaac ccgctcgata cggtcagctg
      421 gcatcgcgtg ctcatgttgg tggaaggggt gggaccaaag aaggcgcagg atctcttggc
      481 gaccttggtc aaatctgaca agccctttca gactctgcgc gaggtaaccg ggcgctctag
      541 cgtgggactc aagagcctgg cgaacacgct ggagagtctt tctggggcag aggacttgcg
      601 tcctgccaat ctggtaagcc aagcctatga gtattatctg ccgattttga aagagcagta
      661 cgacgactat cccaagcgca tacgggatct ggatcatctc cagacgattg ccgaaggcta
      721 tcccggtgtc gatgaatttc tagcagatct ggcgctggag ccgccggatg gcagtgccgt
      781 aggtgtggat gcatccgatc gggatgatga acgtatggtg ttgtcgacga ttcattcggc
      841 caaaggactg gagtggcaat gcgtatttgt gatttgggtg gtggatggca ggttcccgtc
      901 cgtctattcg tttgttgcgg acgacgaatt ggaagaggaa cggcggctgt tctacgtggc
      961 cgtaacaaga gccaagcggc atctttttct gacgtacccg atcaatgtgt tcgataaagg
     1021 aagtggcatg ctgctgtcga aaccctcccg ttttctggac cctgtcacgc ctgcgctgct
     1081 ggatcagctt gtcttggtgg aagaaggtgg gcggcctgac tggtatgctc gcgatgagta
     1141 ctcgttgtga ctgacgcctc ttgagatagc ccttccacca tcgaagcgga ttatgttcat
     1201 ggtgcctgca ttgtgccgag tggtcgcgat cggcttcctc atgggcggac ttctcctcgt
     1261 gcccaatgtc gggaaggcgg aagagcctcg gccgtcaact gaacgaatgt ggcggcagtt
     1321 gctcaatgag gctcatgcgc tgggtcttcc gacaaaattt ctcacggctg ttccaccgag
     1381 cttcgtacgg tttgagttcg acgagttaca cagctatgcc gcagagtatc atctcggggc
     1441 gcacaggatg gtgctcaatc gcacgctgtc cttcaatgga gccggggcga cgttgcgtcc
     1501 gctcgggcga ctcacccatg ccgagatcgc cacgctctat catgagctat tccatgccta
     1561 tctcgactat ttggtgatgg ccgctggggc atcgtccgaa cttgtgttgg atccagtcct
     1621 tgcgtttgcg agggcgcagc aggcctgtcg ttatagtgcg gtcttgatca cgccggtggt
     1681 gcagagaagc agtgagactg aggaacggtt tttgagcgaa cacgaatctt gggaggcact
     1741 caacgaaaca tgggcggtgt ttgtcgggtg ggtggtgtgg aaccaattgg aattgacggg
     1801 ccgtgcaggg caatcgattc agcaatcggg gaaaagtcag gatgaatggg tacgtcgcct
     1861 caggagtgcc gatcgagaag gaatgttccg tgggtattac gaaccggaag gccccgatga
     1921 gcgggccgtg gctcgaaagc gttacttggc tcccgcgtcg cgtttgtcgg aaccggaagc
     1981 cacggtactc atgaaggatg tccttgaatt ttcccccggt ctccttgtgc gggcgagagg
     2041 agaattttct gatacagatc aagtggttat gcatcacgga gcatgccgat aggaaaggac
     2101 atatatcaaa ggccatgaaa tatctgtgag tgactggagg gaggccagat tgctctcgca
     2161 atctagggta ttgagatgtc aaattagaaa agaggaggcc tggcaggaag acaacatgaa
     2221 atttctgacc gttaggcact gtcggagaaa aaaacaagat aaatctactt tttgaccttg
     2281 acattggcat acttcatcaa tagaatccag catttcctga acagactgtg tcctcagttc
     2341 cccatccgca tccttattat ttgtaaggaa ggggaagctg tgcgcggacc agcttgctct
     2401 tctttttcca tcaagcggat gaagaagacc atgattaagc gcttagattt tactatgtag
     2461 ggggttttgg gcaggtaccc aagtggccaa agggggcaga ctgtaaatct gctggcttat
     2521 gtcttccaag gttcgaatcc ttgcctgccc accacatcag gcttgtttag cttggaagtc
     2581 gaaggtttag ataaatacac gatggcatat tgcaggctgc agagcgtggg agaaatgggc
     2641 gggcgtagct cagtggtaga gttccagcct tccaagctgg ctgtcgtggg ttcaaatccc
     2701 atcgcccgct ccatcgaaga cgtgacacgt gaggcgtaaa aagtgaaacg tgggaatgat
     2761 ctgggtcaag tggtttcgag atgtcgtttt acgttttgcg attcacgttt caaggagaag
     2821 gacgcccacg tagctcagtt ggtagagcac gtccttggta aggacgaggt cacgcgttcg
     2881 atccgcgtcg tgggctccag agatttagtc acaggttgag actgaggtcc aggttgagga
     2941 gggaggccga gcaggtcttc tgacccatta tcctgaacct taaccggcaa gtaacggagg
     3001 cgggtatggc gaaggcgaaa tatgagcggc ggaagccgca cgtgaatatt gggacgattg
     3061 ggcacgtgga ccacgggaag acgacgttga cggcggcgct gacgaaggtg tcggcggaga
     3121 aggggatggc caagtttatc
//
LOCUS       DATIPF010000094         2303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1609051, whole genome shotgun sequence.
ACCESSION   DATIPF010000094 DATIPF010000000
VERSION     DATIPF010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609051"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VLL06_02345"
     CDS             complement(<1..248)
                     /locus_tag="VLL06_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29835.1"
                     /translation="MTGPPVTEEELRAMCAAMIARGPDDEGIYLTREIGLAMRRLSII
                     DLHTGHQPVCNEDRSVWVVLNGEIYNYRDLRSDLIKRG"
     gene            complement(309..1547)
                     /locus_tag="VLL06_02350"
     CDS             complement(309..1547)
                     /locus_tag="VLL06_02350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSS29836.1"
                     /translation="MNTGAMIQVQPLEPEYPEEFKLASTVNIPAVDLEVELFRDYEAF
                     VGLESDWNRLVEEAGIDHPFIRHEWVCSWWDCFKPEGSLFIIAVTEGTKMVALAPLML
                     DRGRMYGCPVRRLRGIANIYTERFDFILTRRPEEACRAIWKFLVSHSKEWDVFELRQL
                     IPDAQALEMLPLLAIDDKFLVGTWASNNSPYIPIVRSWDEYEKGLSRKHISKLKGRLK
                     GLSRLGTVSHELVQEEEGLSQAIDDALRLEGAAWKQKAGTAIVCHAGREAFYRRLMLL
                     AARQGWLRLYFLTVNGERVSVQITLLFHNKLYVLKSGYDPHFSTSAPSHMLCWRLLKE
                     AWSLKYDEVDFLGAVERWKMSWTDHVRPHSWLFIFPNRPSGRFLHRLKFRLLPRIQRN
                     GIYRMLIKGGTRVGLKVHDE"
     gene            complement(1648..>2303)
                     /locus_tag="VLL06_02355"
     CDS             complement(1648..>2303)
                     /locus_tag="VLL06_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29837.1"
                     /translation="NRHVFEGLTRLPLRGGGRMFVAGRLAELGIERFRSCYPGAGRRL
                     VDVKQGIGRLLNRLHVERVPVGDTGFNRAHVGIGMAPWSRRLCMGLDYKTIHRRRREN
                     FLRLQEQLPHHAVVRTDLRLGVCPLFFPLLVADKTQASRSLSQRGVMATELWNEGDPS
                     AAGHEGPDAHYLRRHVLELPIHQDITSPHIDYMARQVRELDILLPCSEAVHVQSQIV"
BASE COUNT          536 a          709 c          550 g          508 t
ORIGIN      
        1 tggccacgct tgatcaagtc actgcgaagg tcccggtagt tatagatttc tccgttcaag
       61 accacccaaa cgcttcgatc ctcgttgcac accggttgat gtcccgtgtg caaatcgatg
      121 atgcttaacc gccgcatcgc cagaccgatc tctcgtgtga gatagatccc ttcatcatcg
      181 ggaccccgcg cgatcatcgc ggcacacatc gcacgcagct cttcttccgt caccggaggc
      241 ccggtcatgc tcataatacc ggctataccg cacatagttt ctggttctcc cccaggctca
      301 cttccctgct attcgtcgtg gactttcagt ccaacccgtg tcccaccttt tatgagcatg
      361 cgatagatgc cgttccgttg aatccgagga agcagcctaa atttgagacg atgcaagaat
      421 cggccgctcg gacgatttgg aaaaatgaac agccaggaat gcggccgcac atgatctgtc
      481 cagctcatct tccagcgctc gaccgcacca agaaagtcaa cctcatcata tttcaagctc
      541 cacgcctcct tgagtaagcg ccagcacagc atatgggatg gcgcgctagt cgagaagtgc
      601 ggatcataac ccgatttcaa cacatacagc ttgttatgaa agagcagagt aatttgcacg
      661 gagacccgtt cgccattgac cgtcaaaaag tagagacgta accatccttg ccgagccgcc
      721 agcagcatga gccttcgata gaaggcctcc cggccggcgt gacacacaat ggctgtgccg
      781 gctttttgtt tccaggcagc tccttccagt ctcaatgcgt catctatcgc ctgagacaag
      841 ccctcttctt cttgaacaag ttcatggctc accgtaccga gccggctcaa gcccttcaac
      901 ctccctttta attttgatat gtgcttgcgc gacagacctt tctcatactc gtcccacgag
      961 cgaacaatgg gtatgtaagg actgttgttc gatgcccagg taccgactag gaacttgtcg
     1021 tcgatggcca ataacggcaa catctcaagt gcctgagcgt caggaattaa ttgtcgtagt
     1081 tcaaatacgt cccactcctt tgaatgggat actaggaatt tccagatcgc acggcaggct
     1141 tcctccgggc gacgcgtcag gatgaaatcg aaccgctctg tatagatatt ggcaatgccc
     1201 cgaagacgcc gtaccgggca tccatacatt cggccacgat ccagcatcag cggagcgagc
     1261 gccaccatct tggtcccttc cgtgacagcg ataataaaca ggcttccttc cggtttgaaa
     1321 caatcccacc agctacagac ccattcatgc cggatgaagg ggtgatcaat ccctgcttct
     1381 tccacaagcc gattccaatc cgattccaat ccgacaaagg cctcataatc ccgaaacaac
     1441 tcgacctcca agtccacggc cggtatgttc accgtggatg ccagtttgaa ttcctcagga
     1501 tattccggtt ccaacggttg gacctgaatc attgcccctg tattcatggc ctatccctcc
     1561 tgtcccactc cgggctatgt ccggaggacc ccgacattgc gcgagaccga tccaaacctc
     1621 gaaatcatag aaaggtggat gcgcggatca tacgatctgg gattgaacat gaacggcttc
     1681 tgaacacggc aataggatat ccagctctct cacctgtctc gccatataat cgatgtgtgg
     1741 tgatgtgatg tcctgatgga ttggcagttc caagacatgc cggcgaagat aatgagcgtc
     1801 agggccttca tgtcccgccg cagaggggtc gccctcattc catagttccg tcgccatcac
     1861 accccgctgc gacaggctcc gggatgcctg tgtcttatcc gcgaccaaga gcggaaaaaa
     1921 caacggacag acccccagcc gcaagtctgt gcgcacgacg gcgtgatgag gcaattgctc
     1981 ttgcagcctg agaaagtttt cccggcgacg acgatgaatg gtcttgtaat ccagccccat
     2041 gcacaagcgc ctcgaccagg gtgccatgcc gatgccgaca tgagcccgat tgaatccggt
     2101 atcgccgacg ggcacccgtt cgacatgcag cctgttcagc agacggccga tcccctgctt
     2161 gacatccacc agtcgacgcc ccgctccggg ataacagcta cggaatcgct cgatcccaag
     2221 ttcggcgaga cgtccggcga caaacatcct cccgccgcct cgcagcggca atctggttaa
     2281 tccctcgaac acgtgtcgat tct
//
LOCUS       DATIPF010000095         5999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1432149, whole genome shotgun sequence.
ACCESSION   DATIPF010000095 DATIPF010000000
VERSION     DATIPF010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432149"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VLL06_02360"
     CDS             <1..447
                     /locus_tag="VLL06_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS29838.1"
                     /translation="FQQAYERQMKGELEEAVALYKKSIEAHPTAEAYTFLGWTYSFMG
                     RIEDAIEECHKAIAQDPDFGNPYNDIGAYLIEKGELDEAIVWFQKALLARRYESPAFP
                     HLNLGRVYERKGQWTEAIDSYKKSLALNPSYALAKKALGRLISSLN"
     gene            520..942
                     /locus_tag="VLL06_02365"
     CDS             520..942
                     /locus_tag="VLL06_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HSS29839.1"
                     /translation="MTTNSYEIDQKDKTDRKDQKTILVGVTDIFFYSKVRDALMSKGY
                     RIERARAQQDIADKASTIPSAFIVNMNDDTLNAFQALETLKADARLKTIPILAFANHE
                     EVDTFNRARALGVNKIVSRNEFSTRLKDLIEEVAGDHA"
     gene            939..2033
                     /locus_tag="VLL06_02370"
     CDS             939..2033
                     /locus_tag="VLL06_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HSS29840.1"
                     /translation="MKHTPLYEQHLKLGAVFEEVAGWDMPAHYGKWVAEYQAVRQAVG
                     LSDLSHRGKIRIIGEDRVKWLQSIISNDILPLQPGQGRYSSFLTHKGKMLGYFRVYAL
                     SDSLWVEDVGEVGDATFQALRKFLLYGVKAKMENCAESWGLLLVSGPKACTTVSAAFG
                     VEMSDLTPLSAISARIGGHASFVLRTEETGEIDLEVLLPADALSTAWERLMEAGATYG
                     IKAVGGHAREALRIEAGLPKAGPDLNEEIVPPEANLEGKAFSLSKGCYPGQEVVARMD
                     TYGNVRRKLVGFVLKEATVPPPGAKLFSGDREVGWISSATRSPQLNAVIAFGFPLRDF
                     SEPGTALTVEYDSHKHPATVHTLPFYTKST"
     gene            2279..3118
                     /locus_tag="VLL06_02375"
     CDS             2279..3118
                     /locus_tag="VLL06_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HSS29841.1"
                     /translation="MTQRWMVLIAVLGLMPMLLAMRPVDEALLNAAFKGDLGEVNKLI
                     KNEADVNAANAGGFTPLIAAAEKGHQKIVALLLTQGAEVKQATTDNGTTPLIVAAERG
                     YRKIVEMLLTHGALVKQATTDDGTTALYMAAQQGRRDIVALLLAHGADVRQARTDGTT
                     PLFIAAENGHRGTVALLLDKHADVTQARTDGKTPLFIAVEKEHPATVTLLLDNGADVM
                     QTRMGDGATALHVAASQGDRKIVTVLLEHGADKSAKDTAGQRPVDTARQHGHNALVPL
                     LEP"
     gene            3169..3906
                     /locus_tag="VLL06_02380"
     CDS             3169..3906
                     /locus_tag="VLL06_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HSS29842.1"
                     /translation="MIQQWKVLIAVLGLMPMLLAMRPVDESLLTASSKGDLGEVQTLL
                     NREANVNAANEDGTTPLFIAAEKGHRDVVALLLDNGADVKQTTADGAAPLHIAAEMGH
                     RDVVALLLDNGAEIKQERVDSGEPPLFIAAQEGHREIVALLLEHGADVKQTRTENGAT
                     PLLIAAAKGHRQIVALLLDHGADVTPTTTNGWTALHVAAIAGNREIVALLLKQGADKT
                     AKDTKGLRPVDRARQQGHSTLVALLEP"
     gene            4180..4599
                     /locus_tag="VLL06_02385"
     CDS             4180..4599
                     /locus_tag="VLL06_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HSS29843.1"
                     /translation="MPLDEDGSDVRATPYHAAGELAGITRLVDDFYVNMDTFPEAETI
                     RKMHPPDLTESRKKLTYFLCGWLGGPKLYSQHYGPISIPGFHKQFPIGYEERDAWLFC
                     MQHAIAVQPFDDQFKEYLLAALNIPAERVRQVNSGEI"
     gene            complement(4616..5098)
                     /locus_tag="VLL06_02390"
     CDS             complement(4616..5098)
                     /locus_tag="VLL06_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29844.1"
                     /translation="MAGLMSADEIFDKAHNAAAAATGLDEKPLQIDYPELKNKIRAAL
                     GDRKVALCHINKFLPEGYEDQGRFNLVLLTAGNVLFDMVIGDSYFRYDVLSVGQLDKV
                     QVIDAMWDNKEKRREEPFLSLRLMHGEEAHLLLALEDDERISLLAFARAVSVARNPEK
                     "
     gene            complement(5127..5375)
                     /locus_tag="VLL06_02395"
     CDS             complement(5127..5375)
                     /locus_tag="VLL06_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29845.1"
                     /translation="MLLQVISVVGALMVLSAYGLLQGGIWRELNAGYLAFNIVGSLLL
                     GIVAIVDQRIGFIFLEFAWAGLGLIGVARAMKARHTAS"
     gene            complement(5456..>5999)
                     /locus_tag="VLL06_02400"
     CDS             complement(5456..>5999)
                     /locus_tag="VLL06_02400"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HSS29846.1"
                     /translation="QYRSRNVDQDGPAKTMLGEKQLQWLLSGLAESTATWKVIVTTVP
                     LSISKGGSIVVPGNDGWAGGRVYPGFEWERQVLVDHIVGRRIKNVVFLAGDVHHVQAN
                     AYDPNGDGIPDFHEFVAGPLSAASDRMTPASEGLRPTTLFNERGYFNFGLIRVTTSSF
                     DVKILDDAGTTRFSYHLSAD"
BASE COUNT         1444 a         1712 c         1658 g         1185 t
ORIGIN      
        1 tttcagcagg cttacgagcg ccagatgaaa ggtgaattgg aagaagccgt cgcgctctac
       61 aagaaatcga ttgaagcgca cccgaccgcc gaggcctata ccttcctcgg atggacctac
      121 agcttcatgg ggcggatcga ggatgcgatc gaagaatgtc acaaggccat cgcgcaggac
      181 cctgacttcg gcaaccccta taatgacatc ggggcctatc tgatcgagaa gggtgaactg
      241 gacgaagcga tcgtctggtt tcagaaagcg ctgctggcga gacgctatga gagcccggcc
      301 ttcccccatc tcaacctcgg tcgggtctat gagcgcaagg gccaatggac cgaagccatc
      361 gactcctata agaaatcctt ggcgctcaac cccagttacg ccttggcaaa aaaagcgctg
      421 gggcgattga tcagttcact caactaaccg gtctgtctgg tctattcggt ctatctggtt
      481 tgtctggtga attacttaac gacacattga ggtgtttgaa tgaccacgaa ttcttacgag
      541 atagaccaga aagacaagac agaccgaaaa gatcaaaaaa ccatactcgt cggcgtcacc
      601 gacatcttct tctacagcaa agtccgcgac gccctgatgt cgaagggcta cagaatcgaa
      661 cgggctcgcg cgcaacagga catcgccgac aaggcctcga caatcccatc cgcattcatt
      721 gtcaatatga atgacgacac cctcaacgcc tttcaagccc tggagaccct gaaagccgat
      781 gcacgattga agaccattcc catcctcgcc ttcgccaacc acgaagaagt cgacacgttt
      841 aaccgtgcca gggcgctggg agtgaataag attgtttcgc gaaatgagtt ttcaacccga
      901 ctgaaagatc tgattgaaga agttgcgggc gatcacgcat gaagcacacc cccctctacg
      961 aacagcacct gaaactcggg gcagtctttg aagaagtcgc cggatgggac atgcctgctc
     1021 attatggaaa gtgggtggcg gaatatcagg cggtacgcca ggcagtcgga ctctccgacc
     1081 tctcccaccg tggcaagatc cggatcatcg gcgaggatcg tgtcaaatgg ctccagagta
     1141 tcatcagtaa cgatattctc ccgctccaac ctggccaagg ccgctattcg agctttctca
     1201 cccacaaggg caagatgctc ggctattttc gagtctatgc cctatccgat agcctctggg
     1261 tggaagatgt cggcgaagtc ggtgacgcca cattccaagc cctccgaaag tttcttctct
     1321 atggcgtcaa agcgaaaatg gagaactgcg cggaatcctg gggtctcctg cttgtcagtg
     1381 gccccaaggc ctgcacgacc gtcagcgccg cctttggcgt cgagatgagc gacctcactc
     1441 cgctgagtgc tatatcagcc cgaataggcg gccatgcctc gttcgtcctg cgtacagaag
     1501 aaaccggaga gatcgacctc gaagtattac tgcctgctga tgccctcagt acagcctggg
     1561 aacggctcat ggaggcaggt gcgacgtacg gcatcaaggc cgtcggcggt cacgctcgtg
     1621 aagccctccg catcgaagca ggactgccaa aggccgggcc ggacctcaac gaagagatcg
     1681 tcccaccgga agcgaacctg gaaggaaaag ccttcagctt gagcaagggt tgctatccgg
     1741 ggcaggaagt cgtggcgagg atggacacct atggcaacgt tcgacgaaag ttggtgggat
     1801 tcgtgctgaa agaggcgacc gtaccgcctc ccggggcaaa actcttcagc ggagatcgtg
     1861 aagtgggctg gatcagcagt gcgacgcgct cgccacaatt gaatgccgta atcgcattcg
     1921 gctttccgtt gcgggatttc agtgaaccgg gtacagcgct gaccgtggaa tacgacagcc
     1981 acaaacatcc ggccaccgtc cacaccctgc cattctacac gaagagcaca tagaggtatc
     2041 ctcgcaggcc gcccccggtg cggcctacgt gcgaattgtt cgctcgtgca atgagtcttg
     2101 tcgaacggaa aagccccttc agcgtttccg gcctccgaga tgctctttct cgagatagag
     2161 cgatctgagt gtgacgagaa ccctgagtga ggtcgggtcg gtgagtagag caactgtttc
     2221 agcggcctgt taaagtaaaa ggtaacgtcc cctgtttccc taaccacagg agagcgagat
     2281 gacacaacgg tggatggtgc tgattgcggt gctcggactg atgccgatgc tcctggccat
     2341 gcggccggtg gatgaagccc tgctcaatgc ggcattcaaa ggggaccttg gcgaggtaaa
     2401 caagctcatt aagaacgagg ccgacgtcaa tgcggcgaat gcaggcggct ttaccccgct
     2461 catcgcggca gccgagaagg ggcatcaaaa gatcgtggcg ctgttgctca cccagggcgc
     2521 ggaggtgaag caggcgacga cagacaatgg cacgacaccg ctgatcgtgg cagccgagag
     2581 gggctatcgg aagatcgtgg agatgctgtt gacccacggc gcgctcgtga agcaggcgac
     2641 gacggacgat ggcacaaccg cgctctatat ggcagcccag cagggacgtc gagacattgt
     2701 cgcgctgctg ctggcccacg gcgccgatgt gaggcaggcg agaacggacg gcacaacccc
     2761 gctcttcatc gcagccgaaa atgggcatcg agggaccgtg gccctgttgt tggacaaaca
     2821 cgcggatgtg acgcaggcga gaacggacgg aaagaccccg ctcttcatcg cggtcgaaaa
     2881 ggagcatccg gcgaccgtga ccctgttgtt ggacaatggc gcggatgtga tgcagaccag
     2941 gatgggcgac ggtgcaacag ccttgcatgt cgccgcatct caaggagatc gtaaaatcgt
     3001 gaccgtactg ctcgaacatg gagcggacaa atcggccaaa gacacagccg gacaacgtcc
     3061 ggtggatact gcgcgccagc acggccacaa cgcgctggtg ccgttacttg agccatgaca
     3121 agatggtgcg gggatagctc ccatcagcct aggcacaatg agggccacat gatccagcag
     3181 tggaaagtgc tgatcgcggt gctcggactc atgccaatgc tcttggccat gcggcccgtg
     3241 gatgagtcgc tgctaacagc atcgtccaaa ggggatctcg gtgaagtgca gactctcctc
     3301 aaccgagagg ccaacgtcaa cgccgcgaac gaggacggga cgaccccgct cttcatcgcc
     3361 gccgaaaagg gacatcggga cgtcgtggca ctgctgctgg acaacggcgc ggacgtgaag
     3421 cagacgacgg cagacggcgc tgctccgctc cacatcgctg ccgagatggg acatcgagac
     3481 gtcgtggcac tgctgctgga caacggcgcg gagatcaagc aggaacgggt ggacagtggt
     3541 gaaccgccgc tctttatcgc agcccaggaa gggcaccggg agattgtggc actgctgttg
     3601 gagcacggcg cggatgtgaa gcaaacgagg acggaaaacg gcgcgactcc gcttctcatc
     3661 gccgcagcaa aggggcaccg gcaaattgtg gcactgctct tagaccacgg tgcggatgtg
     3721 accccgacga cgacgaacgg gtggaccgcc ttgcacgtcg ccgcaatagc aggaaatcga
     3781 gagatcgtgg cgctgctcct taaacaggga gcagacaaaa cggcaaaaga taccaaaggc
     3841 ctacgcccgg tcgatcgtgc gcgccaacaa ggccatagca cgcttgtggc gctgctcgaa
     3901 ccttaaacat taacaggaag ctgaaaaagt tcgccagcat cgttctcagc tcatcgaaat
     3961 tctcaacgta ccccagaggg tatgcctccg gtttcgactc gcctgcggcc ttgctggacg
     4021 acctttttga gcatcctgta ggggtgtact gttacttcga gtgcgtaatc ttcagaaaaa
     4081 tcgatggcgg atcatacgga cagaaactct caggttttct tgtatacgaa tctgcctaaa
     4141 cattgtcaga tttcgtaaca accattatag gaaattcata tgccactcga tgaagatgga
     4201 tccgacgtac gcgccacccc ctaccatgct gcgggtgagc ttgcaggaat tacaagactg
     4261 gtggatgatt tttacgtcaa tatggacacg ttccctgaag ccgaaaccat tagaaaaatg
     4321 cacccgccgg atttaaccga atcgcgtaaa aaattgacgt acttcctgtg tggctggctt
     4381 ggtggcccta agctgtattc ccagcactat ggtccaatca gcattccggg tttccacaaa
     4441 caattcccaa ttggttatga agagcgcgat gcctggcttt tttgcatgca acacgccatt
     4501 gccgtacagc cattcgatga ccaatttaaa gaatacctat tggctgcact caacattccg
     4561 gcagaaagag tcagacaggt taactcggga gaaatttgat agcctcaagg atcagttact
     4621 tttccggatt tctcgctacg gagaccgcgc gcgcgaatgc gagcaggctt atgcgttcgt
     4681 catcctccaa cgcaagcaag aggtgcgctt cctccccatg catcaggcgt aggctgagaa
     4741 acggttcttc ccggcgcttc tctttgttgt cccacatcgc atcgatgacc tgaaccttat
     4801 ccagttgtcc gaccgacagc acgtcatagc gaaagtacga atcgccgatg accatgtcga
     4861 acaggacatt gccggccgtg agcaggacga gattgaaccg tccttgatcc tcgtatcctt
     4921 ccggcaggaa cttattgata tggcacagcg cgactttgcg gtcgccgaga gctgcgcgga
     4981 tcttgttttt caattccgga taatcgatct gcaggggctt ttcatcaagc cccgtcgccg
     5041 ccgcagcggc attgtgcgcc ttgtcaaaaa tctcatcagc cgacatcaat cctgccatcg
     5101 tgattccttt catccgaatc gtgttattaa ctcgcggtat gccgtgcctt catggctctg
     5161 gcgacgccga tcagtccaag acccgcccac gcgaactcca agaagataaa tccgatccgc
     5221 tgatccacaa tggcgacaat ccccaatagt aacgacccga cgatgttaaa cgcgaggtat
     5281 cccgcattca gttcacgcca gatcccaccc tgaagcaggc cataggcgga tagcaccatc
     5341 agcgcaccca ccacagaaat tacttgtaac aacatcacgc acctcgtctg aacctcatat
     5401 tcgcgcgcaa cggctaccgt aactggctcc tcgtcctgac ttcaagagga ttctctcagt
     5461 cggcggacag atgataagaa aaccgcgtgg tgccggcgtc gtcgaggatt ttgacgtcga
     5521 aggaggacgt agtgactcga atgaggccaa aattgaaata cccgcgttca ttgaacagcg
     5581 tagtcgggcg taatccctca ctggccggcg tcattcggtc ggatgcggca gataatggac
     5641 cagccacaaa ctcatggaag tcaggaatcc cgtcaccgtt cggatcgtac gcgtttgcct
     5701 gcacatgatg aacgtcgccg gccaggaaca ccacgttctt tatcctccga ccgacaatat
     5761 ggtcgacaag gacctgtcgc tcccactcaa accctggata gacacgccct ccagcccagc
     5821 catcgttccc tggtacgacg atgcttcctc ccttcgaaat ggacagcggc accgtcgtca
     5881 caatcacttt ccacgtcgcg gttgactcgg caagcccgct cagtaaccat tgaagctgct
     5941 tctctcccag catggttttc gccgggccgt cctgatcgac attgcggctt ctgtactgc
//
LOCUS       DATIPF010000096         5697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_17959, whole genome shotgun sequence.
ACCESSION   DATIPF010000096 DATIPF010000000
VERSION     DATIPF010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17959"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /locus_tag="VLL06_02405"
     CDS             <1..1297
                     /locus_tag="VLL06_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HSS29847.1"
                     /translation="LEDVAKDLNEDAQSTAFSGTISLIIGGLVAVVVGLGIGYWIAQF
                     FSKNLGHIAQVAQLAAGGNYQSRAKVTTKEEIGQMATAFNTMLDRIITSLKTTESERD
                     DMQKRLMVFLVLVSEVGKGDLTKRGEVTADVFGNLADGFNLMTQRFGQLMKQVREAAE
                     RVNKSALTLRDSANQMAGTAKQQADESVKTLTAVDQLASSMRQVSATAGASSESAKQV
                     LHATEQGRLAVQETVQDMQNIRSAVQRMSKQVKALGDRSLEISTIVSTIRDIANQTNL
                     LALNAAIEAAGAGEAGVRFAVVADQVRKLAESSTQATREIADLVKVIQTETQDTVVAM
                     EQETQAVEAGSASALRTGEVFNEISGIAQRSSELAQTIASAAVNQTASTDQVGRSLKE
                     FAGGAVATQKATDSARVTVEDMAKLAEGLTSSVAQFKLV"
     gene            1349..4723
                     /locus_tag="VLL06_02410"
     CDS             1349..4723
                     /locus_tag="VLL06_02410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HSS29848.1"
                     /translation="MSSELDRQGLVEIFVIEASEAVDMLTKAFHPSDGTTPTPAQLQE
                     QYVWAHKIRGASALYGYEGLAMMGALLESTLEEGASIEASLWPKALEILRGMVTSFES
                     QLKVVAQGGAEDLSVSARWKGEVAGLFPSVPAAASPEPTLLAPAYLVPTLDAEVLSYF
                     TPEANEYLQSMESFVHRLRQDPKDGESAHALYRIVHTLKGSAYTVGFNVIGDVIHPVE
                     DYMIAVREGGGILSSAVLGIIDRAIEVIRLLMRRDANKVNQLQREVPEVIQALAQIRH
                     GEQMTESVQAAAPMAQVATVIIDHPEESAGPDVESGSPSTILTDEYLLPPLDAEVLSY
                     FAPEAQEYLESLEAQLLRLEKEQGNPDVINQLFRTAHTLKGSAYTVGFQSVGDLTHYI
                     EDFMGAVRDGRIKILPGHADVLLRSVDVVRLLMRRDPMSLDMLRQRYAVAMQGLKQLD
                     QPVAAQVMEPGSLVHPAEALLTQDVRDQQDVEPTKVGEGKTAEGKTVEDREVIRVSRD
                     RLERLLNLVGELVIGRGRLEQRLHMLEQLSQQVLACKGRLVESVHSFEEKHTFTLPSA
                     SSGQASPPAQGFTNITDFGSLEFDKYDDFNILARRISEVTADISESMTQLSGSIRRSH
                     DDMSHLAQLTLGMRDEIARARMVPIGTPFTRFRRATREMARATGKEVTLVTSGEHTEV
                     DTGVVERLVDPLIHLVRNAVYHGIEPAGVRVSKGKPAAGTIYLHAAHRGSAVLIEVED
                     DGAGLDIEKIRAKAVERGLIRPEVARSLPESEIIKFIFMPGFTTADAIGDQAGRGVGM
                     DVVKRVIESMNGHIDVESILGVGTKFTLSLPLTLLIATALMVRAGTERYGVPLPAVRE
                     VTMLTPSSHQRMGERSILHIGEEAIEVQPLQQLLYRSSVPVETGKPVVIVRTAAGMIG
                     MLVDELLGRQEIVIKPLGSFKSLDRSSFGGATIDPEGRVVLVLDPARLLAREAQITAA
                     SDVSSVGTGSAGSMSPYSEIPELKTTEQWLLLIDDSLSIRKFVSRMLESAGYKVHTAV
                     DGEEGLRMASAQDYRLILTDLEMPKVNGYEVIQGLRSRVQTQQTPIIVMTTRAGDKHR
                     QLALNIGASSYIAKPVEERALIQEVERLIGKEAASR"
     gene            4794..5303
                     /locus_tag="VLL06_02415"
     CDS             4794..5303
                     /locus_tag="VLL06_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HSS29849.1"
                     /translation="MTLRGRHNSTNTLVETASFLVVRLGASYLALPAAGVRGVLTEAR
                     HTQSVTALGDIYHPIDLAQRLSVIADLSGLEMRTVLYSNDHAQGAIRVEQVVGLTDVE
                     RKDCFPLPAQFQSDEQHWFMGMMLYQDQLVLILNPAWLLGELGVGVAASVGRTEQISA
                     TPSTVGGSC"
     gene            5297..>5697
                     /locus_tag="VLL06_02420"
     CDS             5297..>5697
                     /locus_tag="VLL06_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HSS29850.1"
                     /translation="MLRATRGQQQAQATKRTWNMVLFSVGGIKLAARTEDVGGISPWT
                     RSISVPSRTPFVHAMLKREHDVIPVYDLATRLSRTLQGDPLLCLVARHLDGPMAICID
                     AEVPSLETVDVVAIRPSGSEDIETLGMVTIGG"
BASE COUNT         1309 a         1409 c         1752 g         1227 t
ORIGIN      
        1 tctggaggat gtggcgaagg atctcaacga agatgcccag tccacggcgt tcagcggtac
       61 gatcagcttg atcatcggcg gactcgttgc agtggtggtc ggactcggaa tcgggtactg
      121 gattgcccaa ttcttttcga agaatctggg ccatattgcg caagtcgcgc agttggccgc
      181 cggaggcaac taccaatccc gtgccaaagt cacgacgaaa gaggagatcg gtcagatggc
      241 gacggcgttc aataccatgt tggatcgcat tatcacgtct ctcaagacaa cggaaagtga
      301 gagggacgac atgcaaaagc ggttgatggt cttcctcgtc ctggtgtcgg aagtcggtaa
      361 gggtgacctg acgaagcgag gtgaggtgac agccgacgtg ttcgggaact tggccgacgg
      421 attcaatctc atgacccagc ggttcgggca gttgatgaaa caagttcgtg aggcagccga
      481 acgtgtaaac aagtcggctc tgaccctccg agacagcgcc aatcagatgg ccggcactgc
      541 gaagcaacag gccgacgaat cggtcaagac attgaccgca gtcgatcagt tggcgtcttc
      601 catgcgtcag gtttcggcca ctgccggcgc ttcgtcggaa tctgccaagc aagtgctgca
      661 tgcgacggaa caaggacgtc tggccgtgca agaaaccgtg caggacatgc agaacattcg
      721 ttcggccgtg caacgtatgt cgaagcaggt gaaggcgctg ggagatcgat cgttggaaat
      781 ctcgaccatc gtgtcgacga ttcgtgatat cgccaatcaa accaacttgc tcgcgttgaa
      841 cgcggccatc gaggcggccg gagcaggcga ggccggagtc cgatttgccg tcgtcgccga
      901 ccaggtgaga aaactggccg aaagctcgac tcaagccacc agagaaatcg ccgacctcgt
      961 gaaagtgatt caaacggaaa cgcaggacac agtcgtcgcg atggagcagg aaactcaggc
     1021 ggtggaggcc ggatcggcct cggcgctgcg taccggtgaa gtgttcaacg aaatttcagg
     1081 gattgctcaa cgttcgtcgg agctggccca gacgattgcc agtgctgcag tgaaccagac
     1141 tgcttcgacg gatcaggtgg gccgttcgtt gaaggaattc gcaggcggtg ccgtcgcgac
     1201 gcaaaaagcc acggactcag cgcgtgtgac cgtcgaagat atggcaaagc tcgctgaagg
     1261 gctgacgtca tccgtcgctc agttcaaatt ggtgtaggtc ggcttcgcgg gacatccgct
     1321 ggatgcttcg gaaaagaaaa ggacagggat gagttccgaa cttgatcgac agggcctcgt
     1381 cgagatcttc gtcatcgagg catcggaagc ggtggacatg ctcacaaagg cgttccatcc
     1441 atccgatggg acaacaccaa ctccggcaca gctgcaagag cagtacgtat gggcgcataa
     1501 aatacgagga gcgtcagccc tttacggata cgaggggttg gccatgatgg gggcattact
     1561 tgaatcgacc ctggaagaag gggcgtcaat agaggcctcc ttgtggccga aggcattgga
     1621 gatcttacgt ggaatggtga cctcattcga gtcccaatta aaagttgttg cgcagggagg
     1681 tgccgaagat ctgtcggtga gtgcgcgatg gaagggcgaa gtggccggat tgttcccatc
     1741 cgtgcctgcc gcggcttctc ctgaaccaac cttgctcgca ccagcctatc tcgtgccgac
     1801 tctcgatgcc gaggtgctgt catatttcac tccagaagcg aatgagtatc tgcaatccat
     1861 ggagtccttt gttcatcggc tacgccagga ccccaaggat ggagagagcg cccatgcact
     1921 gtatcgtata gtccacacac tcaaaggatc tgcgtacacg gttggattca acgtaatcgg
     1981 tgacgtgatc catccggtcg aagattatat gatcgcggtc cgcgagggtg gggggattct
     2041 ctcgtcggca gtccttggga tcatcgatcg ggccatcgaa gtcattcgat tgttgatgcg
     2101 ccgggatgcc aacaaggtca accaactcca gcgtgaagtg ccggaagtca ttcaggctct
     2161 ggcgcagatc cgccacgggg agcagatgac agagtcggtt caagcggcgg ctccaatggc
     2221 tcaggttgcg acagtcatca tcgatcaccc agaagaatca gccggccccg atgtggaatc
     2281 aggttctccg tctactatct tgaccgatga gtatttgttg ccacctctcg atgccgaagt
     2341 gctttcctat tttgctccgg aagcacagga atacctcgag tcgctcgagg ctcagttgct
     2401 tcgactcgaa aaggaacagg gcaatccgga cgtcatcaat caactattcc ggacagctca
     2461 cacgctgaag gggtccgcct atacggtagg attccagtcg gtcggcgatc tcactcatta
     2521 tatcgaagat ttcatgggag cggtgcgcga cggccgcatc aagattctcc cagggcatgc
     2581 ggatgtgctc ttgcgatcgg tcgatgtggt tcggctgtta atgcggcggg atcccatgtc
     2641 tctcgatatg ctgcgccagc gatatgcggt cgcgatgcaa gggctcaagc agttggatca
     2701 gccggtggca gctcaggtga tggagcctgg ctcattggtt catcccgccg aagcgttgct
     2761 cacacaagat gtacgtgatc aacaagacgt tgaacctacc aaagtgggag aaggtaaaac
     2821 ggcagaaggt aaaaccgtag aagatcgcga agtgatccgc gtcagtcgcg atcgcttgga
     2881 acgattgttg aatctggtcg gtgagctcgt catcggccgc ggacgacttg agcagcgact
     2941 gcatatgtta gagcagcttt cccagcaagt actcgcgtgc aagggccgac tcgtcgagtc
     3001 agtccattcg ttcgaggaaa aacatacctt tacgcttccg agtgcttcgt ccggtcaagc
     3061 aagtccgcca gctcaaggat ttacgaatat cactgacttc ggcagcttgg aattcgacaa
     3121 gtacgacgat tttaatatct tagctcgccg catcagtgag gtcacggcgg atatctcaga
     3181 gtcgatgacg caattgagcg gatcgatccg gcgttcacat gatgacatga gtcacttggc
     3241 acaactgacc ctcgggatgc gggacgaaat cgctcgggct cgcatggtgc caattggcac
     3301 gccgtttaca cgattccgtc gtgccacgcg tgagatggcg cgcgcgacag gaaaagaagt
     3361 gacgttagtc acgtccggag aacataccga agtcgatacg ggtgtggtgg agcggttggt
     3421 ggatccgttg attcacctcg ttcgcaatgc ggtctatcac ggaatcgagc ctgcaggtgt
     3481 tcgtgtatcg aaggggaagc cggcagccgg cacaatttac ctccacgcgg cgcatcgagg
     3541 gagcgcggtg ttgatcgaag tggaagacga tggggcaggc ctcgacatcg aaaagatcag
     3601 agccaaggcg gtcgagcgcg gattgatccg acctgaggtg gcccgttcgt tgccggagtc
     3661 agaaatcatc aagtttatct tcatgccggg atttacgaca gccgacgcga tcggagatca
     3721 agctgggcga ggtgtcggta tggatgtggt caaacgtgtt atcgaaagca tgaacggcca
     3781 catcgatgtc gaatcgattc tcggcgtagg gacgaagttt accttgagtc tcccgctcac
     3841 attattaatt gcaacggctc tcatggttcg ggcagggact gagcgctatg gggttcctct
     3901 gccggcggtg cgtgaagtca ccatgttgac acctagttcg catcaacgga tgggcgaacg
     3961 atcgattttg catattggcg aggaagccat cgaggtgcag ccgctccagc agctcctcta
     4021 tcggagcagt gtgccggtag aaaccgggaa gccggttgtc atcgtgcgaa cggcagctgg
     4081 aatgatcggt atgttggtcg atgagctgct cggccgtcaa gaaatcgtga ttaagccgtt
     4141 ggggtccttt aagtcattgg atcgctccag tttcgggggt gcgaccatcg atccagaagg
     4201 tcgtgtggtg ctcgtgttgg atcctgctcg attgttggcg cgagaggcac aaattactgc
     4261 ggcatcggac gtgtcgtcgg tcggaacggg atcggcaggt tcgatgagcc catactcaga
     4321 gataccagag ctgaaaacga ctgagcaatg gctgttattg atcgacgact ccttgagtat
     4381 ccgaaagttc gtgagccgga tgttggagtc ggctgggtat aaggttcaca ccgcggtgga
     4441 cggagaagaa ggtcttcgta tggcctctgc gcaagattat cggctgatcc tcaccgatct
     4501 tgagatgccg aaggtgaacg gctatgaagt cattcaggga ttgcgcagtc gagtgcaaac
     4561 gcaacagaca ccgatcattg tgatgaccac gcgagccggt gacaagcata gacaattggc
     4621 acttaatatc ggagcaagct cgtatattgc aaagcctgta gaagagcgag ccttgattca
     4681 ggaagttgaa cgcttgatcg gcaaagaagc cgcctcgcgc taaagtagaa tccagagcga
     4741 ccgatccttg aaggagtctc tggcaagcga ccgtctaata tgatggctca actatgacgt
     4801 tacgaggtcg tcacaattcg accaatacac ttgtggagac cgccagtttc ttggtcgtcc
     4861 gtcttggtgc cagctatttg gcgcttccgg cagcgggggt tcgaggtgtt ctcacagaag
     4921 ctaggcacac acagtctgtg acggcattgg gggatatcta ccatccgatc gatctcgccc
     4981 aacgcctttc ggtgatcgcc gatctgtccg gtctcgagat gaggacggta ttgtactcga
     5041 acgaccatgc gcagggtgcg attcgcgttg aacaggttgt gggcttgaca gacgtggaac
     5101 ggaaagattg ttttccgctg ccggcacagt ttcaaagcga tgaacaacac tggtttatgg
     5161 gaatgatgct gtatcaggat cagttggtgt taattctcaa tcctgcgtgg ctgctgggag
     5221 agttgggtgt cggtgtggct gcgtccgtag ggcggactga acaaatttcg gcaaccccat
     5281 caactgtggg tggatcatgt taagggctac tcgtggacag cagcaggctc aagccacgaa
     5341 acgaacgtgg aatatggtgc tattttctgt cggtgggatc aagctagccg cccggacaga
     5401 agacgtgggg gggatatcgc cctggactcg gagcatttca gtgccgagtc ggacgccatt
     5461 tgtgcacgcg atgttgaaga gggagcatga tgtgataccg gtctacgatt tagcgactag
     5521 attgagtcgc acacttcaag gagatccatt actgtgtctg gtcgctcgcc atcttgatgg
     5581 gccgatggcc atctgcatcg atgccgaagt gccgtctctc gaaacggtgg atgtggtggc
     5641 gattcgaccg agcggcagcg aggatattga aacactgggg atggtgacaa ttggggg
//
LOCUS       DATIPF010000097         3706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_636161, whole genome shotgun sequence.
ACCESSION   DATIPF010000097 DATIPF010000000
VERSION     DATIPF010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636161"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /gene="fliD"
                     /locus_tag="VLL06_02425"
     CDS             <1..1071
                     /gene="fliD"
                     /locus_tag="VLL06_02425"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HSS29851.1"
                     /translation="HQITNKGATAVASSTTDIVSGGSASFTFHVGTGTDKTVTLSDTA
                     TLQDLQTGINDLGAGVTASIINTGSDTTPAYRLILTATASGASNGVTIVTDGTTLDFT
                     NGGGTGGVDTLQAAQDAIAIVGDPTLNPVTLQRSSNVITDAIPGVTLSLTNTTGTNTV
                     SVNVTRDNGAVKTNIQNFVKAYNDIVTFVNKDTAYDVTTKTGATLYNETSVKGFVSQL
                     RSALSAPIGGLTTYGSVGEIGFQTNRDGTITVDNTKLDGALNSNYNAVKSLFINQTTV
                     AGVAQRVNQAIDAMTGAVSGSLTGRKNALAGQINTLTDQISRKETDLSAYEERLKIQY
                     AKLDSLLSQLQGQTTYLNSLGK"
     gene            1071..1481
                     /gene="fliS"
                     /locus_tag="VLL06_02430"
     CDS             1071..1481
                     /gene="fliS"
                     /locus_tag="VLL06_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HSS29852.1"
                     /translation="MNTYSPTTAATHYRQTQVLTASGVQLIVLLYDSAIQAVELASDG
                     IARRHQSDKARFLGRAVGIVSELSSVLDFERGGEVAKSLYRLYDYMLSEFTQANLRND
                     ARHLDGPLRCLKEMRGAWHTVAQQQNQPMSMSRG"
     gene            1595..1924
                     /locus_tag="VLL06_02435"
     CDS             1595..1924
                     /locus_tag="VLL06_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29853.1"
                     /translation="MNPTNQMVWEQNVRTLTMQASQAAELGRWDQVEECFRLRGEQLR
                     DHSIAPTLAADLAVLDREVETRIVNARLAVQSQLIEAAKIRQRFLGLRSWQGRSETQR
                     PLMTKVA"
     gene            2016..2609
                     /locus_tag="VLL06_02440"
     CDS             2016..2609
                     /locus_tag="VLL06_02440"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS29854.1"
                     /translation="MLPPSAKPAKSLSDERREWIRIDDRVLLEYRLLTDPADGPTPGI
                     PPVAQEAIAAVISKPTSDLLLRSGEQLAESPLLPWIRKVDWVLETIVKTLAKMQPEGI
                     AIAQIADVTLSGGGIGFLSPRQFAVDDTLRLKIILSPFTPIHTTARVIRSVQAKDGIN
                     YDIATEFIQLNPDDQEHLIRHIIQTQAERLRGRRNIG"
     gene            complement(2665..3558)
                     /locus_tag="VLL06_02445"
     CDS             complement(2665..3558)
                     /locus_tag="VLL06_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HSS29855.1"
                     /translation="MPIIISIASGKGGVGKSVIAANLALLLAKRGKRVTLADLDVGGS
                     DIHILFGHMNPPLTLTDFLSRRVERLEDIVQALPFHPGLTLIPGTGDTLATANMPYAK
                     KKRLIRHLKDLQADIVVVDIGAGTSYHALDFFLMADCHVTVATPDPTSVLDLYRFIKL
                     AAIRRVLSSFLARDAMSDALSDRDFCSVEEVLEVAGHTDEAGRVIAETTLRTFHPALI
                     LNRLSGRSHVNVSALKKLLAEYIGGQLTLLGEIPDDPSMERAVRSFLPLAEYEPASPA
                     AAALRTIADRLANYVGEMAET"
BASE COUNT          883 a         1025 c         1062 g          736 t
ORIGIN      
        1 catcagatta ccaataaggg agccacagcc gttgccagtt ccaccaccga tatcgtcagc
       61 ggcgggtcgg ccagctttac gttccacgtc ggaacgggaa ccgacaagac ggtcaccctg
      121 agtgacaccg ccacgctcca agacttacag accggcatta acgacttggg cgcgggcgtg
      181 acggcgtcga tcatcaacac cggcagtgac acgactcccg cgtatcggtt gattcttacg
      241 gccactgcgt ctggggcgag caatggagtc acgattgtga ccgacggcac gacgttggat
      301 tttacgaatg gtggcggcac aggaggggtc gataccttgc aggctgcgca ggacgccatc
      361 gcgatcgtcg gagacccgac actcaacccc gtgactttgc agcgaagtag taatgtgatt
      421 actgacgcga ttccaggtgt cacactctca ctcaccaaca cgacgggaac caacacggtc
      481 tccgtcaatg tgacacgcga caatggcgcg gtgaagacca acatccagaa ttttgtcaag
      541 gcctacaatg acatcgtgac gttcgtgaac aaggacacgg cctatgatgt gacgacaaaa
      601 acaggggcaa ctctctacaa cgagaccagc gtgaaaggat tcgtatccca actccgatcg
      661 gcgctctctg ctcccatcgg cgggctgacc acgtacgggt cggtgggtga aatcgggttt
      721 caaaccaatc gtgatggcac gattaccgtg gataatacga aactggatgg cgcgttgaac
      781 tccaactata acgccgtcaa gtcgctcttc atcaatcaga ccacggtggc cggtgtcgcc
      841 cagcgagtca accaggccat tgatgcgatg actggtgcag tttcagggtc gcttactggc
      901 cgcaagaacg cgctggccgg acaaatcaat accttgacgg atcagatcag caggaaggaa
      961 acggacctat cagcgtatga agaacggctc aagattcagt acgcaaaact cgatagttta
     1021 ttgagccagc tacaagggca gaccacctac cttaactcac tgggaaaatg atgaatacct
     1081 attcacccac gaccgccgcc acgcactatc gacaaacgca ggtcctgacc gcatccggcg
     1141 ttcaacttat tgttcttctg tacgactcgg ccattcaggc tgtcgaactc gcgagcgacg
     1201 ggattgcgcg acgccatcag tcagacaagg ctcggtttct cgggcgagcc gttgggattg
     1261 tcagtgaatt gtccagcgtg ctcgacttcg aacgaggtgg ggaggtcgcg aagtcgctct
     1321 accgtctcta tgattatatg ctctcggaat ttacgcaggc caacctgcgt aacgatgccc
     1381 gccatctcga tgggccacta cgttgcttga aggaaatgcg cggggcctgg cacacggtgg
     1441 ctcagcaaca gaaccagccg atgtccatgt cgcgaggata gcccgcattg tttatggcgg
     1501 ttcgttgctc cagcccatcc ctccccaaat catgagggag gggggaaggg atgggcgatc
     1561 acggcagtat ggtccataaa tactgagggc aagcatgaat ccaacaaatc agatggtctg
     1621 ggagcagaac gtacgcactt tgacgatgca ggctagtcag gctgccgagc tcggacggtg
     1681 ggatcaggtc gaggaatgtt tccgactgcg tggggagcaa ctcagagacc attcgatcgc
     1741 tccgacgctt gccgcagatc tggctgtatt ggaccgagaa gtcgaaacca gaattgtgaa
     1801 tgctcgtttg gcggttcaat ctcaactgat cgaggcagcg aaaattcgcc agaggtttct
     1861 ggggttacgg tcgtggcaag ggcggagtga gactcaacgt cctctcatga ccaaagtggc
     1921 atgatcagcc gacacgaatt gccatggaga ccgaatgcct ctggaaacag attgatgctc
     1981 cagcatcttc gtaggaaggt ccgatgagcc caaccatgct gccaccatca gccaaaccgg
     2041 ccaaatcctt gtcggacgag cggcgggaat ggattcgcat cgacgaccgg gtacttcttg
     2101 agtatcgtct cctgaccgac cctgcagatg ggcccacgcc gggcattccg cctgtcgctc
     2161 aagaagcgat cgcggccgtc atctcgaagc cgacatccga cctattgctc cgcagcggcg
     2221 aacagctggc agaatcgccg ctccttccct ggatcaggaa agtggactgg gtgttggaaa
     2281 cgatcgtgaa gacgcttgcg aagatgcagc cggaaggcat cgcgatcgca caaatcgccg
     2341 atgtgacgct gagcgggggc ggcatcgggt ttctctcgcc acggcaattt gccgtcgacg
     2401 acacactgcg actcaaaata attctctcac cctttacacc gattcacacg acagcacgag
     2461 tgattcgatc cgtccaagcc aaagatggca tcaattacga cattgcgacg gagtttatcc
     2521 agctcaatcc ggacgatcag gagcatctga ttcgtcacat cattcagaca caggccgaac
     2581 gattgcgcgg ccgccgcaat atcggctaga ggaaaggccg gcgacaagag tgaagggcca
     2641 gaagccgtcg ggttacaaac atcttcaagt ttccgccatc tctccaacat agttggccag
     2701 tctgtcggca atggtgcgaa gcgccgcggc cgccggagag gccggctcgt attcggccag
     2761 cggtagaaag cttcgtacgg cacgttccat cgatggatcg tcaggaatct cccccagcaa
     2821 cgtgagctga ccgccgatat actcagcgag aagctttttg agtgccgaca catttacgtg
     2881 ggagcgtccg gacagtcgat tgagtattaa ggccggatga aacgtccgga gtgtggtttc
     2941 agcgatcact cgtcctgcct catcagtgtg ccccgccact tcgagcactt cttccacact
     3001 gcaaaaatca cgatcagaca gtgcgtcaga catggcatcc cgagcgagga acgaggacaa
     3061 gacgcgacgg attgcggcaa gcttgatgaa gcgatagagg tccagcaccg aggtagggtc
     3121 cggtgtggcg acggtcacat ggcagtctgc catcaagaaa aaatccaacg cgtgatagct
     3181 cgtgccggcg ccgatatcaa cgacgacgat gtcggcctgc agatctttca gatggcgaat
     3241 gagccgtttc ttcttcgcat agggcatgtt cgcggtcgcg agcgtatccc ccgttccggg
     3301 aatgagtgtg aggcctggat ggaagggaag ggcctgcacg atgtcttcga gccgttcaac
     3361 tcgccggctc agaaaatctg tcagggtgag tgggggattc atgtgaccaa acaggatgtg
     3421 gatatcggac ccgccgacat ccaggtcggc cagcgtcaca cgtttgcccc gttttgccag
     3481 taacagcgct agattggcag cgatcacact cttgccgacg ccccccttcc cggacgcgat
     3541 ggagatgatg atgggcattg agggagcttc ctactggttc gtgacggttg tactccgtct
     3601 tcggctgatt cggaagaaaa catgagcgat cgcctcacac agatgagagg gcgacggacg
     3661 atgatcgatg gtgcaatgag cgtcatcggt gactcaagtt ttctat
//
LOCUS       DATIPF010000098         3620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1168268, whole genome shotgun sequence.
ACCESSION   DATIPF010000098 DATIPF010000000
VERSION     DATIPF010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168268"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VLL06_02450"
     CDS             complement(<1..185)
                     /locus_tag="VLL06_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29856.1"
                     /translation="MLKPLVLVLESVVADAAAPGQVELPVAVEEGASPGRVALVQSLM
                     AQAETWMDSEALVFPEK"
     gene            230..829
                     /locus_tag="VLL06_02455"
     CDS             230..829
                     /locus_tag="VLL06_02455"
                     /inference="COORDINATES: protein motif:HMM:NF037406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29857.1"
                     /translation="MHAIPSAKPAPQMQESLPMPPQSQPSPSKLVGASLHQDPAPPPL
                     QSGSSPVDLVKTLCGRFHAVARQLRLRGEYRATLSVEDEFDAQDLLHALLRIQFDDIG
                     TDEWMPSYTDGAPRTTLLLDDGRLAVIVKKTRPGLNAKDLGDQLRVDAERYRSRDRAT
                     TLLCFMYDPEGRIGNPRGLEASLTSVNESFMIDVLVAPK"
     gene            838..1245
                     /locus_tag="VLL06_02460"
     CDS             838..1245
                     /locus_tag="VLL06_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSS29858.1"
                     /translation="MRKPKLAAVTRPTSSAGSSALTQTTGIRYVGHDEISAVPCTIHA
                     PVDGGRIMSDRAYVLINVLPGRTSDVVRALSEIKQIKTIDPCWGKPDIIVVVEVADQD
                     ALTRLVLSRIHEIEGVMQTDTHLVYRLKVSHAK"
     gene            complement(1375..2778)
                     /locus_tag="VLL06_02465"
     CDS             complement(1375..2778)
                     /locus_tag="VLL06_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSS29859.1"
                     /translation="MQPVNILVADDDAVARELLAEALKKEGYAVEAFASGEEVIARGR
                     QGRVDLVLTDIRMGAVDGLTVLREFKRMSPDTAVVVLTSFGSLEGAIEAIKQGAYDYL
                     AKPFKKEDIKLVVKRSLDHCRLVRENARYRQELKSKDEWSPLVGSSPAMLEVYKLVAR
                     VANSKSTVLLQGESGTGKELVARAIHANGPRRDKPFVPVNCGALPDTLLESEMFGYEK
                     GAFTGSVGHKVGLFESANGGTLFLDEIGELGQAVQVKLLRVMQDHEVRRVGSTTSTKV
                     DVRIIAATNRDLEQLVKEGKYRDDLFYRLNVVRITLPALVERREDIPMLVHHFFEKYA
                     VGAAHAVRGVLPETMTLLMQHRWPGNVRELENAIERAVSLSHGPLLTPDDLPASLHQT
                     ELPLKETTGAIGHTDERCLSLEEVEKRHLVRVLKETKGNKVKAAKILGIDRRTLYRMA
                     ERFGLDLGDDADSGEFT"
     gene            complement(2829..>3620)
                     /locus_tag="VLL06_02470"
     CDS             complement(2829..>3620)
                     /locus_tag="VLL06_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS29860.1"
                     /translation="LARETENLLKANTQLVSAKDQLNRSERLAAVGQLVASVAHEVGT
                     PLHSIAWHVQALAEEPGVTPEMKKRVTVIDEQLTRVVRIIQDLLSSTRPRLPEPTWLP
                     VEQVISPAAVLMEPAFHAKEITLTVEIPKDLPLVWADKEKLHQVLVNVLANALAATPP
                     HGTVSVSAGVRAASSDELDRGRRVVNAMSPQVIAVAVRDTGCGMPETDVQKAFEPFFT
                     TKAVGKGTGLGLFLSRETVLAHGGNLLLESDTSQGTTVTVTLPAL"
BASE COUNT          750 a         1137 c          935 g          798 t
ORIGIN      
        1 tgtttctctg ggaaaaccag agcctctgag tccatccacg tctcagcctg agccatcagc
       61 gattgtacca gcgccacccg cccaggcgat gccccctcct ccaccgcaac cggtagctcg
      121 acctgccccg gcgccgctgc atcagccacc acagactcca gaaccagcac aagcggcttc
      181 agcacctacc atcaggccac tgccaccacg tcatgaaccg gagctcatca tgcatgcaat
      241 tccctctgcc aaaccagcgc cccagatgca agaatcgctt cccatgccgc cgcagtcgca
      301 gccgagtccc tccaagttgg tcggggcatc cttgcatcag gatccagcac ctccgcctct
      361 tcagagcgga tcgagcccgg tcgatcttgt gaagacactc tgtggtcggt ttcacgccgt
      421 cgctcggcag ttacgactcc gtggggagta tcgagccacg ctcagtgtgg aggatgagtt
      481 cgatgcgcaa gatctcctgc atgcactcct acgaatccaa ttcgacgaca tcggtacgga
      541 tgaatggatg ccgagttata cagatggggc cccgcgcacg acgctcttgc tggatgacgg
      601 tcgacttgcg gtgatcgtca agaaaacacg tcctggtctc aacgccaaag acctgggcga
      661 ccaactccgg gtcgatgcgg aacggtatcg gtcccgtgat cgcgccacca cgctcttgtg
      721 ttttatgtat gatcccgaag gtcgtatcgg aaaccctcga ggcctcgaag cgagtctcac
      781 aagtgtgaat gagtcattta tgatcgatgt ccttgtcgct ccgaaatgag gtgatgaatg
      841 agaaagccca aacttgccgc cgtcacgcgc cccacatcgt ccgcaggctc ctccgcattg
      901 acacagacaa caggcatccg ctacgttggc catgacgaga tttctgcggt gccttgtacg
      961 attcacgctc cggtcgatgg tgggaggatc atgtctgatc gggcctacgt gcttatcaat
     1021 gtattaccgg gacgtacgag tgatgtggtc cgggccctgt ccgaaatcaa gcagatcaag
     1081 accatcgatc cctgctgggg caagccggac attatcgtgg tggtggaagt ggctgaccaa
     1141 gatgctctga cccggttggt gttgagcagg attcatgaga tcgagggcgt catgcagact
     1201 gatacgcatc tcgtctatcg attgaaggtc agtcacgcca agtgaccagg accctcctta
     1261 attatgtgag cataaacgtg cttcggtggg agaattcacc ggaccgctgt cccagtgtgc
     1321 caagaatcga gggcgggtgg ttacgagctt ccagcaaacc ctgtatacag tctcttacgt
     1381 gaactctccg ctgtcggcat cgtcaccaag atccaatcca aaccgttcag ccatccgata
     1441 gagcgtgcgc cggtcaatcc cgagaatctt cgccgctttg accttattcc ccttggtttc
     1501 cttcagtacc cggacgaggt gccgcttctc gacttcctca agcgacaaac acctctcatc
     1561 agtgtggccg atcgccccgg tcgtctcctt caatggcagt tctgtctgat gcagtgatgc
     1621 cggcaaatca tcaggcgtga ggaggggacc atgactcaat gagacagccc gctcaatggc
     1681 attttccaac tcacgcacat tgccgggcca tcggtgctgc atcaacaatg tcatcgtttc
     1741 cggcaacact ccgcgcaccg catgcgccgc ccccaccgca tatttttcaa agaagtggtg
     1801 caccaacatc ggaatatcct cccgccgctc cacgagcgcc ggcaaggtga tacgcacgac
     1861 attgagccga taaaataaat cgtcacggta tttcccttct ttgacgagct gctcgagatc
     1921 tcgatttgtc gcggcaatga tacggacatc gaccttcgtc gatgtcgtgc tgccgacacg
     1981 gcggacttca tgatcttgca tgacccgcaa gagcttgact tgcacggcct gccccaactc
     2041 gccgatctcg tcaagaaaca acgtgccccc gttcgcggat tcaaagagac cgactttgtg
     2101 ccccaccgag ccggtaaagg ctcccttttc gtacccaaac atttccgatt ccagtagggt
     2161 gtcaggcagc gccccacaat tcaccggaac gaatggcttg tcccgacgag gtccattcgc
     2221 atggatcgcc cgcgccacca gctccttgcc ggtcccgctt tccccttgca ggagcaccgt
     2281 gctcttgctg ttggccaccc gcgccaccag cttatagact tccagcatgg cgggactgct
     2341 tcccaccaac ggcgaccatt catctttgct cttcaattcc tgacgatacc gggcattctc
     2401 tcggacgagg cggcagtgat cgagactccg cttgaccacc agcttgatat cctctttctt
     2461 aaacggttta gcgagatagt cgtaggcccc ttgcttgatc gcttcgatcg ctccttccag
     2521 agatccgaac gaggtcagta ccacgacggc tgtgtcgggg ctcatacgtt taaactcacg
     2581 caggacggtg agcccatcga ccgcgcccat acgaatatct gtcagaacca gatccactcg
     2641 tccctggcgc ccacgagcga tgacctcttc tccactggcg aaggcttcaa cggcatagcc
     2701 ttctttcttt agggcctcgg ccaatagttc gcgcgcgacc gcatcgtcgt cggctacaag
     2761 aatattcact ggctgcatta acccctctcg atcacgggcg ttggctcggt cttcaatcca
     2821 atataaggtc acagtgcagg taacgtcacc gtgacggttg tgccctggct ggtgtcgctc
     2881 tccagtaaca ggttgcctcc atgggccagg actgtttcgc ggctcaagaa tagtcctaat
     2941 ccagtcccct tgccaaccgc cttggtcgta aaaaatggct caaacgcttt ctgcacatca
     3001 gtctccggca tgccgcatcc cgtatcgcgc accgcaaccg cgatcacctg aggtgacatc
     3061 gcattgacca cccggcggcc acggtcgagt tcatcggacg aggccgcacg gactcccgcg
     3121 gatacgctca cggttccatg cggtggggta gccgctaagg cattggctaa tacattcacc
     3181 aacacttgat gcagcttctc cttatcggcc cagacgagtg ggaggtcctt gggaatttcg
     3241 acggtcagtg taatttcttt cgcatgaaag gccggttcca tcaatactgc tgcggggctg
     3301 atcacttgct ccacgggcaa ccaggttggt tcaggtaggc gcggtcttgt ggaggacagc
     3361 aaatcctgaa tgatccgaac cacccgggtg agctgttcat cgatcaccgt cacccgcttc
     3421 ttcatctcag gtgtgacgcc cggctcttcg gccagtgctt ggacgtgcca ggcaattgag
     3481 tgcagcggcg ttcctacctc atgtgccacg gacgccacca attgtcccac cgctgcgagg
     3541 cgttccgacc ggttcagctg gtctttcgct gagaccaatt gggtattggc cttcaagaga
     3601 ttttccgtct cccgggcgag
//
LOCUS       DATIPF010000099         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_372049, whole genome shotgun sequence.
ACCESSION   DATIPF010000099 DATIPF010000000
VERSION     DATIPF010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372049"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VLL06_02475"
     CDS             <1..795
                     /locus_tag="VLL06_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HSS29861.1"
                     /translation="TETFTQWWKGRGTAQIPASASRKVALFGSCLINYQSTDIGKATI
                     QVLEKNGVHVVIPEQRCCGMPSFDLGDTVAIQQAASANIASFLPWMEKGYEIVVPVPS
                     CSLMWKREYPEIVGGPDAQRVAERTFDVSEYLMKLKREGALATDFQRKPGRVAYQVPC
                     HLRDQNIGFKSKELMECAGAQVEVIEKCSGHDGSWSAKTEFFPLSMLIAQKAVRAIEQ
                     APADLVASDCPLAGLQLDQAGAMTHAGGRPTKHPIQIVRDAYGLRL"
     gene            801..1373
                     /locus_tag="VLL06_02480"
     CDS             801..1373
                     /locus_tag="VLL06_02480"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HSS29862.1"
                     /translation="MSPQDLLPAPEYELQRERFRSQIIALKQRRRISVGPLITLVFEN
                     RETLRFQTQEMIRVERILEPHKVQEELDVYNVLMPGSGELSATLLIEITEQDRMKEWL
                     DVFMRLDHGDTVAIRVGAERAFGVFEGGHSHETKISAVHFVRFRPTASMITAFADLHV
                     PVSLTVHHHEYHAEASVPGSMREEWLQDLR"
     gene            1375..2127
                     /locus_tag="VLL06_02485"
     CDS             1375..2127
                     /locus_tag="VLL06_02485"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HSS29863.1"
                     /translation="MASVLLTKRIEFAAAHRYMKAEWDEAKNRAVFGPCYNPPAHGHN
                     YMVEVTVAGEVDPKTGMVVNLFDLKRILLHVLEEFDHKNLNLDMPYFEDRIPTSENLA
                     RVLWTKLDMQKAIGTLHAIRLYEDEDLYADVTAVAGLDVASVTRRYSFTAVQDGGPGP
                     QWDCFVTVHGTIDPVTGMVTDIGALDRLVQERVVQTFDRHDLRRVLGTPTVTGEQLTE
                     HIWRQLVSSLSSGRLSNVCLVQSRDLSFEYAG"
BASE COUNT          456 a          577 c          651 g          474 t
ORIGIN      
        1 acggagacat ttacccaatg gtggaagggt cgagggacag cacagattcc tgcctccgcg
       61 tcgcgaaagg ttgcactctt cgggagctgt ctgatcaatt accaatcgac cgatatcggc
      121 aaagcgacga ttcaagtcct cgaaaaaaac ggtgtacatg tcgtgattcc cgagcagcgt
      181 tgttgcggca tgccctcatt cgatctcggc gatacggtgg cgattcagca ggctgcgtcg
      241 gccaatattg cgtcgttttt gccctggatg gagaaagggt acgagattgt cgtgccggtg
      301 cccagttgca gcttgatgtg gaagcgggag tatcccgaaa tcgtcggtgg gcctgacgcg
      361 cagcgcgtgg cagaacggac gttcgatgtg tcggaatatc tcatgaaact gaagcgggaa
      421 ggcgctctgg ccactgattt tcagcggaag cccggccgag tggcctacca ggttccttgc
      481 catctgcggg accagaacat cggattcaaa tccaaggaac tcatggagtg tgccggggct
      541 caagtcgagg tgattgagaa gtgttccggc catgacggct catggtccgc caagacggag
      601 ttcttccccc tttccatgct gatcgcgcag aaggcggttc gagcgataga acaggctccg
      661 gcagatcttg ttgcgtccga ttgcccgctg gcgggattgc agttggatca agccggcgcg
      721 atgacccacg ccggcggcag gccgacgaag catccaattc aaattgtgcg tgacgcctat
      781 gggttgaggc tatgaacccc ttgtctcctc aagacctctt gcccgcgcca gagtacgaac
      841 tacagcggga gcggttccgc tcgcagatca ttgcgctcaa acagcgtcga cggatttccg
      901 tggggccgct catcaccctc gtcttcgaaa atcgtgagac cttgcggttt cagacacagg
      961 aaatgatccg ggtcgaacgc attctcgagc cccacaaagt ccaggaagaa ttggacgtct
     1021 ataatgtcct catgccgggg tccggtgagc tgagcgccac cttactgatc gagattacgg
     1081 agcaggaccg gatgaaagaa tggctcgatg tattcatgcg gctcgatcac ggtgacactg
     1141 tcgcgatccg cgtaggggcc gaacgggcct tcggggtgtt tgaaggcggc catagtcatg
     1201 agaccaaaat cagcgcggtg cattttgtcc gattccgccc gactgcgtcg atgataaccg
     1261 cgttcgccga cttgcatgtg ccggtctcgc tgacggtgca tcatcacgaa taccatgccg
     1321 aggcctctgt cccagggagt atgcgtgaag agtggcttca agatctgagg tagtatggca
     1381 tcggtgttac tgacgaagcg catcgagttt gcggcagccc atcgctatat gaaggctgaa
     1441 tgggatgaag ccaagaaccg tgcagtgttc gggccctgtt acaacccacc ggcgcatggg
     1501 cataactaca tggtcgaggt cacggtcgcg ggcgaagtag atccgaagac gggtatggtc
     1561 gtgaatctgt tcgatttgaa gcgcatcttg ctccacgtgc tcgaagaatt cgatcataaa
     1621 aatttgaacc tggatatgcc ctacttcgag gaccggattc ccacgtcgga aaaccttgcc
     1681 cgtgtcctct ggacgaaact ggatatgcaa aaagccatcg gtacgctgca cgcgattcgg
     1741 ctgtacgagg atgaagatct ctatgcggat gtgacggcgg tggccgggct cgacgtcgcc
     1801 agtgtcacgc gacgctactc gttcaccgcg gtgcaggatg gcgggccagg tccccagtgg
     1861 gactgcttcg tgacggtcca tggcaccatt gatcccgtga ccggtatggt cacggatatt
     1921 ggtgctctcg accggttggt ccaggaaaga gtcgttcaaa cgtttgaccg tcatgacctt
     1981 cgtcgggtcc ttgggactcc caccgtgacc ggcgagcagc tgaccgaaca catctggagg
     2041 caattggttt cttctctctc atcgggaaga ctttcgaacg tttgtctcgt gcagtctcgg
     2101 gacttgtcct tcgaatacgc aggctaaccc atctccgtct gttcagtacg gggggttc
//
LOCUS       DATIPF010000100         4535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1610360, whole genome shotgun sequence.
ACCESSION   DATIPF010000100 DATIPF010000000
VERSION     DATIPF010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610360"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VLL06_02490"
     CDS             complement(<1..519)
                     /locus_tag="VLL06_02490"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HSS29864.1"
                     /translation="MSQKAHYFKIGLFVLGATLLAVVGVIVLGSGRWFENVRMVETYF
                     TESVQGLEIGAPVRLHGVRLGRVESIRLAREEYKIPFDPESGLLPYKGVVVVRMSIQP
                     SIAATMPAGDEEMRIKKAGDAGLRFRLASQGITGVLYIESEFVDPERYPPMQFAWIPK
                     SPYIPSAPSTITE"
     gene            complement(516..1322)
                     /locus_tag="VLL06_02495"
     CDS             complement(516..1322)
                     /locus_tag="VLL06_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSS29865.1"
                     /translation="MEHSDRIPIIQVAHLTAAYDGTVLLDDLSFDVYPAEVFAILGSS
                     GAGKSTLLKHMIGLLRPVRGSILIEGEDITTVEGQERLAFLTKIVVMYQQGALFGSLN
                     LLENVRLPLEEHTDLPDDAIDTIARMKLQLVGLGSATYKLPDEISGGMRKRAAIARAM
                     ALDPRILFLDEPSAGLDPITSAELDELILSLARNLKVTFIVVSHELPSIDTIADRVIM
                     LDKRTKTIIAQGQPKALRDHSENPWVRQFFNPRTNAGVSKPRRQTTSETS"
     gene            complement(1333..2469)
                     /locus_tag="VLL06_02500"
     CDS             complement(1333..2469)
                     /locus_tag="VLL06_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HSS29866.1"
                     /translation="MKSSEPPASVLHVHRPSEEGLVFSIIGPLDSKTTGLAWRTALHS
                     LQLANPRRVIVDAARLTYCDGAGAALLLELHRRQRRGHGTFEIRSLDPAYQALLDLYG
                     RSDGQSPEPATPAATPIEQIGRATVALGNDIVVLIAFVGELCAALGRALRHPGLVRWR
                     DAWLTAELAGVNALPIVALLGFLLGLIMAFQAAIPMRQFGADLYVANLIGLSILRELG
                     PLLTAIILAGRSGSAFAAELGTMKVSEELDALTTMGLEPIGFLVVPRVIAAVAMTPLL
                     AIFAGVFGLIGGAVVMLSLGFPLVTYMNQVQAAVTVGDMIGGLGKSFVFGIVVAAIGC
                     LRGLQTKSGASAVGESTTRAVVSGLVLITLVDGVFAVIFYALGI"
     gene            2779..3108
                     /locus_tag="VLL06_02505"
     CDS             2779..3108
                     /locus_tag="VLL06_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29867.1"
                     /translation="MALHRATVLSLTIRCLLFSWLCFGGLELLEQLNVMQEVEDQDEV
                     ALAQVGSGLKSEVTGPDDTVSAPVANQAAESIAWLSFATAHQFVRLMVHSPPSLRLHQ
                     QLSVYRI"
     gene            3296..3856
                     /locus_tag="VLL06_02510"
     CDS             3296..3856
                     /locus_tag="VLL06_02510"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS29868.1"
                     /translation="MGKTVEYQGYTIQSTPHYEIDWKKWRLCIAVSAEDFLGVRTREF
                     SSEILYTTEQEADIHGIAFGQRLIDGKVIGRSVADMKTTDRRVMPRLHVQFRTTFSDF
                     RNREGGGTMLDLSLGGCRIESPLIVELGVSLELRIHAPDIQWPLMVNAASVQWVSGQT
                     FGLAFFWITDAEKQRLSQVINDLMLD"
     gene            3935..>4535
                     /locus_tag="VLL06_02515"
     CDS             3935..>4535
                     /locus_tag="VLL06_02515"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HSS29869.1"
                     /translation="MENRKLTLQALSQFVHHHLLWLLIGAYAIAAVFPSPGLQIRSLT
                     FGEIPIFHTTIHVSLLLLLLATLMFNAGLGVKTSQLQSLMQKGRVILAGLAANLIIPM
                     LYIFGVTLVMRLWYEPDEAQHILVGLALVAAMPIAGASTAWAQNSNGNLALSLGLVLS
                     STVLSPIMTPVALQMFGEMASDEYETVLQDLAAYGSGTFL"
BASE COUNT          964 a         1306 c         1275 g          990 t
ORIGIN      
        1 ctccgtgatc gtgctcggcg ccgatggaat gtacggggat tttgggatcc aagcgaattg
       61 catcggcggg tagcgctctg gatcgacgaa ctctgactcg atgtagagca ccccggtaat
      121 gccctgtgac gccaaccgaa agcgtaaacc ggcatcgccg gccttcttaa tccgcatctc
      181 ttcatctcct gccggcatcg tggccgcgat gctcggctga attgacatcc gcacaacgac
      241 cacccctttg tatggcaaga gaccgctctc tgggtcaaag ggaatcttgt actcttccct
      301 ggcaagacga atcgattcca ctcgccccag cctgacaccg tgaagcctca ccggtgcgcc
      361 gatttcaagc ccctgaaccg actcggtgaa atatgtttcc accatcctca cgttctcgaa
      421 ccacctgccg cttccaagga caatgacacc gacgacagcc aagagggttg ccccgaggac
      481 gaagagacca atcttgaaat agtgcgcttt ctgactcatg acgtttcgct tgtcgtttgt
      541 cgcctcggct tagacactcc agcattggtt cgaggattga agaactgccg cacccatgga
      601 ttctccgaat gatctcgcag cgctttcggt tgtccctgcg caatgatggt ttttgtacgc
      661 ttgtctaaca tgatgacccg gtcagcgatc gtgtcgatgc ttgggagttc gtgcgatacg
      721 acaatgaacg tgaccttgag gttcctcgcg aggctcaaaa tcagttcatc taattcggcc
      781 gacgtaatgg ggtcgagccc tgccgaaggc tcatccagaa agagaatacg agggtccagc
      841 gccatggcac gggcgatggc cgcacgcttt ctcatcccgc cgctgatctc atccggcagc
      901 ttgtatgtcg cggaccctaa gccgaccaat tgcaacttca tgcgggcgat cgtatcgata
      961 gcgtcatcgg gcaagtcggt atgctcttcc agcggcaacc gcacgttttc cagtagattc
     1021 aatgatccga acagcgctcc ttgttgatac atcacgacaa tcttggtcag gaatgccagc
     1081 cgttcttgac cctccaccgt tgtaatgtcc tcaccctcga tgaggattga accccgcacc
     1141 ggtcggagca atccaatcat atgtttcagc aacgtactct tgccggcccc ggagctcccg
     1201 aggatcgcga acacctcggc cggatagaca tcgaacgaga gatcgtcaag cagcaccgtg
     1261 ccgtcatacg ctgctgtaag gtgcgcgacc tgtatgatcg gtatccggtc tgaatgttcc
     1321 atgttgcctc gctcagatac cgagcgcgta aaagatcacc gcgaacaccc cgtcgacgag
     1381 cgtaatcaac acgaggccgc tcaccaccgc cctggtcgta gactccccca ctgcactagc
     1441 cccgcttttg gtctgaaggc cacgcaggca accgattgcc gctacgacga tcccaaagac
     1501 gaacgatttg ccgagcccac cgatcatatc gccgactgtc actgctgcct gcacctgatt
     1561 catgtaggtc acgagcggaa aacccagcga gagcatgacg accgccccgc cgatgagccc
     1621 gaatacacca gcaaatatcg cgagcagcgg cgtcatggcc actgcggcaa tcacccgagg
     1681 cacgaccaga aatccgatcg gctccaatcc cattgtcgta agggcgtcga gctcctcgct
     1741 cactttcatt gtcccgagct cggcggcgaa agcggagccc gaacgtccgg ccaggataat
     1801 cgcggtcaat agcgggccaa gttcccgcag tatggagagg ccgatcaaat tggcgacata
     1861 gaggtcggct ccgaattggc gcatcgggat ggcggcctgg aaggccatga tcagtcccaa
     1921 gagaaatccc aggagtgcga cgatcggcag cgcgttgact cctgctagct ccgccgtcaa
     1981 ccacgcgtcg cgccaacgaa cgaggccggg atgccgaagc gctcgtccca aggccgcaca
     2041 gagttcgccc acgaatgcga tcaagaccac gatatcgttc cccagcgcaa ccgtcgcacg
     2101 gccgatctgt tctatcggcg tggctgccgg cgtagctggt tcggggctct gaccgtccga
     2161 ccgtccgtag aggtcgagca gtgcctgata ggcgggatcg agagatcgga tttcgaaggt
     2221 tccatgtcca cgcctctgac ggcggtggag ttccagcagg agggccgcac cagctccatc
     2281 gcaataggtc agccgcgcgg catcgacgat cacgcgacgc ggatttgcca gttgcaggct
     2341 gtgcagcgca gtccgccagg ccaggccggt cgtcttagaa tccagcggac cgataatgga
     2401 aaagacgagt ccctcttcgg agggacgatg cacgtgaagg acggacgctg gcggttctga
     2461 ggatttcatc gaagagcaag cctctatcgt ctcaattctt gcatctcctg tatgaaagcc
     2521 ctgtcatggt agcagattta cgtgaatgaa ataagactgt gcttgcgggc attgggcaac
     2581 aagcgcagga gataatctca gagccaccgt tcgccaggga cgtgccactc cagacttacg
     2641 gctcgctccc aggagacgga gttggaagtg ggatatcggc tccgagcgga atgacctcgt
     2701 gcaagtggcc gctgatcgag aagtgtgggc cgaattacca agattgactg cccttcaggg
     2761 gttgactata gtgcgactat ggcactccat cgagccactg tcttgtccct tactatccgg
     2821 tgtctgcttt tctcatggct ctgcttcggt gggctggaac ttcttgagca gctcaacgtg
     2881 atgcaggagg ttgaggatca agacgaagtg gcgctggcac aagtgggctc gggattgaag
     2941 tcagaggtta cgggtcccga cgacaccgtc agtgcccctg ttgccaatca ggcagccgaa
     3001 tcgattgcat ggctgtcctt tgccaccgct caccagtttg tccggttgat ggtccatagc
     3061 cctccctccc tacgtcttca ccaacaactc tccgtgtatc gaatctgatc gttcttccat
     3121 ttcatcgctg agcgcgaggg tgctttgatt tcgcgcgggg gggtcccgtg cgcgcattct
     3181 ttgtccgcca ctggcgctct cgctggacac gcgtctgtat cttcgtgtca ctggccaggc
     3241 cggaacacat cgacagtcat ctacgccaat gcgcccgaaa ggaaggatga agctgatggg
     3301 aaagacggtg gagtatcagg gatataccat tcagtccact ccacattatg aaatagactg
     3361 gaagaaatgg cggctatgca tcgcagtctc tgcggaagat ttccttggcg tgcgaacgcg
     3421 cgagttttca tctgagattc tgtacacgac ggagcaagaa gctgatatcc acgggattgc
     3481 cttcgggcaa cgtctcattg atgggaaggt gataggccgg tccgtcgcgg atatgaagac
     3541 gacggatcgc cgggtgatgc cgcgtctcca tgtgcagttc cgtactacgt tttcggattt
     3601 cagaaatcgg gagggaggcg gaaccatgct tgacctgtct ctgggcgggt gccgtattga
     3661 aagcccgctc attgtcgagc tgggtgtgtc gttggagttg cggatccatg cgccagacat
     3721 ccaatggccg ctgatggtca acgcggccag cgtgcagtgg gtgagtggcc agacatttgg
     3781 gctggctttc ttttggatca cagatgccga gaagcaacgg ttgagtcagg tgattaacga
     3841 tctgatgctg gattagtcgt gggtttgcca cgcccgtaac atatcggtaa tcgcagtcac
     3901 agaaatctcg aagccgatct attggtgatt agtaatggag aatcgcaaac tcacgcttca
     3961 agctctgtcc cagttcgtcc accaccattt gctctggttg ttgatcggcg cctatgccat
     4021 cgcggccgtt tttccgtcgc ctggcttaca gattaggagc ctcactttcg gcgagatccc
     4081 aatttttcat acaacaatcc atgtgtcgtt gctcctgctt ctcctcgcca cgctcatgtt
     4141 taacgcagga ttgggcgtga aaacgtcgca gttacaatcg ctcatgcaaa aaggcagggt
     4201 gatcttggcc ggactcgcag ccaatctgat catcccgatg ctctatattt ttggcgtcac
     4261 cctggtcatg cgtctgtggt atgagccgga cgaagcacag catatcctag tcggtctggc
     4321 attggtcgcc gccatgccga ttgccggtgc gtcaaccgcg tgggcgcaga actcgaatgg
     4381 aaacttggcg ctgagcttgg gattggtcct gtcctcaacc gtgctgagcc cgatcatgac
     4441 accagtggcg ctgcagatgt tcggggagat ggcatcggat gaatatgaaa cggtcctgca
     4501 agacctcgcc gcctacggat cgggaacgtt cctgg
//
LOCUS       DATIPF010000101         5397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1696334, whole genome shotgun sequence.
ACCESSION   DATIPF010000101 DATIPF010000000
VERSION     DATIPF010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696334"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VLL06_02520"
     CDS             <1..346
                     /locus_tag="VLL06_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29870.1"
                     /translation="VRIDATDSIAAFSEDLVDKKDDLIKRREYARLDASIVSPNRIEI
                     KTDRVQRYSLFLNEQLIDSSKPLVVLTNGQVSFEGSVTPSLETLLRQTRLRQDSRQLF
                     PIQLIIQVRKQP"
     gene            343..1161
                     /locus_tag="VLL06_02525"
     CDS             343..1161
                     /locus_tag="VLL06_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29871.1"
                     /translation="MMIPESLCSRSAGLLSVLAVIGLSTTMYPDVVRSETCVLASQHA
                     GVTFPVEQIEARWACPLQAVISNYTTVNKVGPILTPIPEPVYRYLLDRPPMAAALTNR
                     LDLSLYKSEARGPGRFWCSDGEGTEGLVELVYQDPTNRVYYVEGTHTSLVLPDINGKA
                     IVLITMNPVQDGQGNDAVETTLVAYARLNSRVLAGLVSLIRPLISNTITRKLTKGFNV
                     VNRLGQEMRQHPDRVLFEATDPPPLPHDEVAFLKQALEQLSHAGSTPQLRMPQP"
     gene            1158..2327
                     /locus_tag="VLL06_02530"
     CDS             1158..2327
                     /locus_tag="VLL06_02530"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSS29872.1"
                     /translation="MTTSRSFTLLCTVGVFCFISYNMVRMPVLALFAESLGASPERVG
                     LIVSVSTLTGVFLKLPSGAQSDIYGRRFLLQIGVMAFGLPPFLYPWITDLDTLTALRF
                     FHGLATAIFAPSALATVADLYTARRGAALGTYTASTQSGALLGPLIGGYLIYASGFSE
                     AFVTAGIFGCIAIAIFYSLRLTPPPPRIQEKGMAPLLAEMWKGFRVVAKNRKVLITSL
                     TDAAKMIANGALMAFLPLYGVSAGLNPGEVGLLFTVQALTSLVSKPIMGRVSDSIGRQ
                     PLIVIGLVFCAATFVCIPQTSMLWLLLLLSAGFGFGEAVVSSSSSALVADSSEFKTLG
                     AGMGMQGTIGDIGHAAGPLLAGILIARLNYADAFAIIAGLQLVAAAVFWVTMRRA"
     gene            2342..2464
                     /locus_tag="VLL06_02535"
     CDS             2342..2464
                     /locus_tag="VLL06_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29873.1"
                     /translation="MIKAIATENLVIVVLTSIGVVVLLVMFVYVVKQMFGQKGE"
     gene            2522..3178
                     /locus_tag="VLL06_02540"
     CDS             2522..3178
                     /locus_tag="VLL06_02540"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HSS29874.1"
                     /translation="MFTGIVEELGAIISIDKTLAGTRMAILASGVMADLKVGDSISVN
                     GTCLTVVNKAEHNFSVEVSPETLSVTTLGQLGAGAPLNLERAMKLNERLGGHLVAGHV
                     DGIGTVRSRQQDGNAVLFMIEAPPEILRHCVVKGSITVDGISLTINQVTDRGFSVSII
                     PHTAKVTTLGLKQVGDAVNLESDLIGKYVERLLQERGQIPAKSAPVIDQDYLKKHGLL
                     "
     gene            3351..4088
                     /locus_tag="VLL06_02545"
     CDS             3351..4088
                     /locus_tag="VLL06_02545"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HSS29875.1"
                     /translation="MASKVSVPKRLTVDRLLSKRGIASRGTSQEWIRAGRVRINGRVI
                     RDPATWVLWPKDAVSIDDRPIQDSVKRFILFHKPRGVITTHSDEKGHKTIFDVLPADL
                     GYIHAVGRLDQATSGLLLLTNDSTLSSLLTDPIHKVMRTYLVSVRGEFTEVQAAEAVA
                     GVVDGGERLQCHTVKIQKSSGRESHLEVVLIQGKNREIRRLFQALGHEVTRLRRIQYG
                     PFKLDDLPSGGWREIPIEDVRKALRQS"
     gene            complement(4157..4663)
                     /locus_tag="VLL06_02550"
     CDS             complement(4157..4663)
                     /locus_tag="VLL06_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29876.1"
                     /translation="MQYVAMAILMALIGFDLSLADAAPPEQRESLRGLQGVEVVIEDI
                     KSDAQVDGLSQEALRAAVELIIRSRGIRVLTPSERSEMPSKPYLYVHVGTDKNSSGQY
                     AFNARVALHQAVSLVHRPQHIILAPTWSTPDKFRTVGQQTMRMWIINSIEPLVREFVD
                     DFRAVNPR"
     gene            complement(4748..>5397)
                     /locus_tag="VLL06_02555"
     CDS             complement(4748..>5397)
                     /locus_tag="VLL06_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HSS29877.1"
                     /translation="IGGAYLCVYGMEGPGGYQFVGRTVQMWNRYRQTADFKDGKPWLL
                     RFFDQLRFYPVTESELLELRRDFITGSFKLRVEETTFSLKQYNRFLIENAASITAFKT
                     GQQVAFEAERERWKQKGQADYVSEATLDKADAQSELNLPEGAHAVSAHVPGTVWKLLV
                     NPGQHVTAGNPLLIIESMKMEIAVDAPVNGTVGQVFCKVSGNVSAGQMLLVIQED"
BASE COUNT         1094 a         1549 c         1526 g         1228 t
ORIGIN      
        1 ggtgcgcatc gacgcgaccg attccattgc cgcgttctcc gaagatctgg tcgacaaaaa
       61 agacgacttg atcaagcgcc gggaatacgc gagactggac gcctccattg tgtcgccgaa
      121 tcgcattgag atcaaaaccg accgggttca acgctacagc ctcttcttga atgagcagct
      181 catcgattct tccaaaccgc tagtcgtgct cacgaacggg caggtgtcct tcgagggctc
      241 cgtgacgcca tcgcttgaga ccttgctgcg gcagacacgt ctgcggcagg attcccggca
      301 actctttccg atccagctca tcatccaggt tcgaaaacag ccgtgatgat tcccgaatcg
      361 ttgtgttcgc gaagcgccgg cctcctgtcc gtgcttgccg tcatcgggtt gagtacgacg
      421 atgtatccgg atgtggttcg atcggaaacc tgtgtgctgg catctcaaca tgcaggagtg
      481 acctttccag ttgaacagat tgaggcacgc tgggcctgcc ctctgcaggc ggtcatcagc
      541 aattacacca cggtcaacaa ggtcgggccg atcctcactc ccattccaga acccgtctac
      601 cgctatctct tggaccggcc accgatggct gccgcgttga cgaatcgact cgatctcagc
      661 ctctacaaat cggaggcgag gggacctggc cgtttttggt gcagcgacgg cgagggaacg
      721 gaggggctcg tcgagcttgt ctatcaggat cccaccaatc gcgtttacta tgtggaagga
      781 acccacacaa gcctcgtgtt gccggacatc aacgggaagg ccatcgtatt gatcaccatg
      841 aatccggtac aagatggaca ggggaacgac gcagtcgaaa cgacgctggt cgcctatgcg
      901 agactgaaca gccgtgtgct ggccggactg gtctcgctca tacgaccact gatcagcaac
      961 accattacac ggaaactgac caaaggcttc aatgtcgtga accgtctggg tcaagagatg
     1021 cggcaacatc ctgatcgtgt cctcttcgaa gcaaccgatc ctccaccgtt gccgcacgac
     1081 gaagtggcct tcttgaaaca ggcattggag caactgtcgc atgccggcag cacaccacaa
     1141 ctccggatgc ctcagccatg acgacgtcgc gcagcttcac cctgctctgc accgtcggcg
     1201 tgttctgctt tatcagctac aacatggtcc ggatgccggt gctggcgctg ttcgcggaat
     1261 cgctgggggc cagccccgaa cgggttgggc tcatcgtgtc ggtgtccacc ctcaccggcg
     1321 tgttcctcaa actgccgtcc ggcgcgcagt ccgacatcta tggccggcgg ttcctgctgc
     1381 aaatcggtgt catggccttt ggactcccgc catttcttta cccttggata accgatctcg
     1441 atacgctgac tgccttgcgc ttcttccacg gactggcgac cgccatcttt gcgccgagtg
     1501 cgctggcgac tgtcgccgat ctctataccg cacggcgagg cgctgcgctg ggcacctaca
     1561 cggcctccac gcaatccggt gcattgctcg ggccgctcat cggcggctat ctcatctatg
     1621 cgtcaggatt ttctgaggct ttcgtgactg ccggcatatt cggctgcatt gccatcgcca
     1681 tcttctatag tctgcggctg acgccacctc ccccgcgtat tcaggagaag gggatggccc
     1741 cgctgctcgc agaaatgtgg aagggcttcc gggtggtcgc gaagaatcgc aaggttctca
     1801 ttacgagctt gaccgacgcg gccaaaatga ttgcgaacgg ggccttgatg gcttttctcc
     1861 cgctctatgg tgtctcggcg gggctgaatc ctggtgaagt aggcctgctg tttacggttc
     1921 aggccctcac gtcgctcgtc tccaagccga ttatgggccg ggtgtctgac agcatagggc
     1981 gtcaaccgct catcgtaatt ggcctcgtgt tctgtgcggc gaccttcgtg tgcattcccc
     2041 agacgtcgat gttgtggctg ttattgctgc tctcggcggg cttcggattc ggcgaagccg
     2101 ttgtctcctc ctcctcgtcc gcgctggtcg cagatagttc cgagttcaag acgctcgggg
     2161 cggggatggg gatgcaaggg acgatcggag acatcggtca tgccgccgga cccctcctgg
     2221 cggggatcct gatcgcacgc ctgaactatg ccgacgcgtt cgccatcatc gccggtctgc
     2281 aactcgttgc agcggcggtg ttctgggtga cgatgagacg ggcgtagtct ataatgggct
     2341 gatgatcaag gccattgcca cagagaatct tgtcatcgtc gtgctgacga gtatcggggt
     2401 cgtcgtgctg ctcgtgatgt ttgtctatgt ggtgaagcaa atgttcggtc agaaagggga
     2461 ataggctccc aaggcgacga cgaggcgaga ttgaagttgg gaccctgtgt aaaatcctgc
     2521 tatgttcaca ggcattgttg aagaacttgg cgctatcatt tcgatcgaca agaccctcgc
     2581 cggaacgaga atggcgattc tggcctcagg ggtcatggcc gacctcaagg tcggggacag
     2641 catcagcgta aatgggactt gtctgaccgt ggtgaacaag gctgagcaca acttttccgt
     2701 cgaagtctct cccgaaacat tgtccgtgac cactctcggg caacttgggg ctggcgcacc
     2761 gctcaatctg gaacgggcga tgaagctcaa cgaacggctc ggagggcacc tcgtggccgg
     2821 tcacgtagac ggcatcggca cggttcgcag ccgccaacag gatgggaatg ccgtcctctt
     2881 catgatcgaa gccccgccgg agattctgcg ccactgcgtc gtgaaggggt cgatcaccgt
     2941 agacggcatc agcttaacga tcaaccaagt gacagatcgc ggtttttccg tctcgattat
     3001 tccgcacacg gcgaaggtca ccaccttggg cctcaagcaa gttggcgacg cggtcaatct
     3061 cgaatcggat ctgatcggga aatacgtcga acgtctgctg caggaacgag gacagattcc
     3121 cgccaaatcg gctccggtga ttgatcagga ctatctcaaa aaacacggcc tcctttagca
     3181 ggatgctgaa aaggtccgcc agcggcgttc tcggctcatc gaaatcctca acgtacccaa
     3241 gagggtacgc ctccggtttc gattcgcctg cggccttgct ggacggcctt tttgagcatc
     3301 ctgcaactgt cgatattcgc cgatcagctt tcaggtagat aggtttctgc atggcatcca
     3361 aggtctccgt gcctaagcgt ctgactgtgg atcggctgct gtcgaagcgt gggattgctt
     3421 ccagaggcac gagccaggaa tggatccgtg cagggcgagt ccgtatcaat ggtcgtgtca
     3481 tccgcgatcc ggccacgtgg gtcttgtggc caaaagatgc tgtctcgatt gacgaccgac
     3541 cgattcaaga ttcagttaaa cgatttatcc tttttcataa gcccagaggg gtgatcacga
     3601 cacacagtga cgaaaagggc cataaaacga tcttcgatgt gctgccggcg gatctgggat
     3661 acatccatgc cgttgggagg ctcgatcagg cgaccagtgg ccttctcctg ctcaccaacg
     3721 attcgacact gtcaagccta ctcacagatc cgatccataa agtgatgagg acctacctgg
     3781 tctctgtgcg aggggaattt actgaagtgc aagccgcaga ggccgtagcc ggagtggttg
     3841 atggtggcga gcgcctccag tgccatactg tgaagattca gaagagttcc ggacgcgaat
     3901 cacatctgga agtggtccta atccagggga agaaccgaga aattcgcaga ctgtttcagg
     3961 cgttggggca cgaagtgacc agactcaggc gcattcaata tggtccgttc aaactggacg
     4021 atcttccctc cggcggatgg cgcgagatcc cgatcgaaga cgtgaggaaa gccctccgac
     4081 agtcctaacc cgtcgttcgt ctctcgggct catgatgctt ttctgttatg ggcgaggccg
     4141 tgctctcagc acaatgctac cgaggattga cggctcgaaa gtcgtcaaca aattctctga
     4201 cgagcggctc aattgaattg atgatccaca ttcgcatggt ctgttggccc accgttcgga
     4261 atttatcagg agtggaccag gttggtgcaa gtatgatgtg ctgcggccgg tgcaccaatg
     4321 atacggcttg gtgaagcgcc actcttgcat tgaacgcata ttgccccgat gaatttttgt
     4381 ctgtaccgac atgcacgtac agatacggtt tggagggcat ctcggatcgt tctgatggag
     4441 tcaggactcg tatccctcgg gatcgaatga taagttcgac ggctgcgcga agcgcctctt
     4501 gggacaagcc gtccacttgt gcgtctgatt tgatatcctc gatgaccact tcaaccccct
     4561 ggagcccccg caacgattcc cgctgttcag ggggtgctgc gtcagccaga ctcaggtcga
     4621 agccgatcag ggccatgaga attgccatcg cgacatactg cataggacct ccagggttag
     4681 gcaatctcct gcctgttggt gggttgcttc tccaccggac atggctcatg tgtataccgg
     4741 tcgtcgctca atcttcctgt atgacgagca gcatctgccc ggcggataca ttcccactga
     4801 ccttacaaaa tacctgcccg actgtgccgt tgactggtgc atccactgca atttccatct
     4861 tcatcgattc aatgatcagc agcgggtttc ccgccgtcac gtgctgcccc ggattgacga
     4921 gcaatttcca caccgtgccg ggcacatgtg cactgacggc atgcgcccct tccggcagat
     4981 tcaactcact ctgtgcatcc gccttgtcga gcgtggcttc gctcacataa tctgcctggc
     5041 ccttctgctt ccagcgttcg cgttcggctt cgaatgcgac ttgttgcccg gttttgaatg
     5101 cggtgattga tgcggcattc tctattagga acctgttgta ctgtttcaag ctgaacgtgg
     5161 tttcttccac gcgcagctta aagcttccgg taataaaatc cctccgcagt tcgagcagtt
     5221 cgctttcggt cacgggatag aatcggagtt ggtcgaaaaa gcgcaggagc cacggcttgc
     5281 cgtccttgaa atcggcagtc tggcggtagc gattccacat ttgcaccgtg cgtccgacga
     5341 actggtaacc gccgggacct tccatgccat acacgcacag ataggctccg cctatcc
//
LOCUS       DATIPF010000102         4033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_105491, whole genome shotgun sequence.
ACCESSION   DATIPF010000102 DATIPF010000000
VERSION     DATIPF010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105491"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /gene="hemC"
                     /locus_tag="VLL06_02560"
     CDS             <1..590
                     /gene="hemC"
                     /locus_tag="VLL06_02560"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HSS29878.1"
                     /translation="FMDLPHGARIGTSSLRRQAQLLHARTDLTIAMLRGNLDTRLRKL
                     REGQFDAIVLAAAGLRRLAWAHEITEYLAPQVSLPAIGQGALGIEGRRDDLFIHSLLS
                     GLDHAQTRTAVLAERALLHRLQGGCQVPIAAHATVVGTGVRLEGLVSSVDGKELIRDT
                     VEGAIEDPESIGIQLAERLLARGGDRILQAIYGTT"
     gene            587..2128
                     /gene="cobA"
                     /locus_tag="VLL06_02565"
     CDS             587..2128
                     /gene="cobA"
                     /locus_tag="VLL06_02565"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HSS29879.1"
                     /translation="MTKLRRPGKVYLVGAGPGDPGLLTLKGKECLEQADIVLYDYLAN
                     PVLLQYAPATAQRIYVGRRGRGQYQDQADINRLLIERAKEGNVVVRLKGGDPFVFGRG
                     GEEAEAVSAGGVDFEVVPGVTAAVAVPAYAGIPVTHRTLASTVTFVTGHEDPTKPGTQ
                     LEWPKLASASGTLVFMMGMKNLPTITRQLLSEGRSPDTPVAAIRWGTKADQQTIVGTL
                     NDIVEKTEAAHLEPPTAIVIGEVVRLRGQLNWFEAKPLFGRRIVLTRPQKQAREFSQL
                     LSGYGAEPVEVPTIQIVPPASWQAIDDAVTRLGTYHWLIFTSVNGVKPFMERLHVARK
                     DVRALAHLRLCAIGPRTAQELGTYGLTSDVVPTQYQAEGVIAALADAGVRGSHILIPR
                     AEVAREILPEQLRELGATVDVIPVYRTIAPAVDATSLTKQFCDGQVAAVTFTSSSTVR
                     NFVEVFGGRDAVKSLVARVVIACIGPITARTAEEYGLTVTIMPAENTVPALAEAIVKH
                     FKEVA"
     gene            2206..2598
                     /locus_tag="VLL06_02570"
     CDS             2206..2598
                     /locus_tag="VLL06_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSS29880.1"
                     /translation="MVSVKSFMIPREKFVTVERDMNVQTAARIMRDRGIGSLFVTNGK
                     EIIGILTDTDMVRRVVAAGADTQKTTVEQVMSAPLLAIDENKSLLDANDLMAKTHTRH
                     LGVTQDGRIVGMISVRDLVLFLTNLPRK"
     gene            2603..3574
                     /gene="hemB"
                     /locus_tag="VLL06_02575"
     CDS             2603..3574
                     /gene="hemB"
                     /locus_tag="VLL06_02575"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HSS29881.1"
                     /translation="MAFPIQRLRRLRQHESFRGMVRETRLAPSDLIYPLFVVEGRDRR
                     EEITSMPGQFRLSVDLLVQEVGEIAALGIPAIILFGIPDRKDDRGSAGFDPNGIVQRA
                     VKAVKGQVPGLIVVTDVCIDEYTDHGHCGIVKDGRILNDETLECLRAMARTHAQAGAD
                     MVAPSDMMDGRVAAIRGELDQAGFPELPIMAYAAKFASCFYAPFREAACSSPQFGDRQ
                     SYQMDPANRREALREIALDVDEGADIIMVKPALPYLDIIAAAREQVLLPVAAYQVSGE
                     YSMIKAASRAGWLDESRAMMESLLSIKRAGADLILTYFAKDAARLLR"
     gene            3580..>4033
                     /locus_tag="VLL06_02580"
     CDS             3580..>4033
                     /locus_tag="VLL06_02580"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29882.1"
                     /translation="MLKVTRGLSDQRPRAYPVATIGNFDGHHLGHRALLQTVVETARK
                     AQGTSLVLTFEPHPVRILAPHVDLHFLTSPEEKLAHLEVTGIDEVVFLGFTPAFAAMS
                     PDQFAEDILHRSLGISELFVGNHFAFGKGRAGRIDDLVRLGGRYGFQVH"
BASE COUNT          858 a         1020 c         1274 g          881 t
ORIGIN      
        1 cttttatgga tcttcctcat ggggcgcgaa tcgggaccag cagtcttcgc cgccaagcac
       61 agcttctcca tgccaggaca gacctcacga ttgccatgct gcgagggaac ttagataccc
      121 gcctcaggaa attgcgagag ggacagttcg acgccatcgt gctggccgca gcggggctca
      181 ggcgactagc ctgggcgcat gaaattacgg agtatcttgc gccacaggtt agtttgcctg
      241 ccattggcca gggagccctg ggcatcgagg gacggcgaga cgatcttttt atccattcgc
      301 tgttaagtgg cctggatcac gctcagacca gaacagctgt tctcgccgaa cgtgcgttgc
      361 tccatcggct ccaggggggc tgtcaggttc cgatcgcggc ccatgcaacg gtggtcggga
      421 cgggggtgag attggaagga ctagtatcca gtgtggacgg taaagaactc attcgcgaca
      481 cggttgaagg agccattgag gatccggagt cgatcggtat acaacttgcg gaacgactgc
      541 ttgcacgcgg tggtgatcga attctccaag cgatttacgg aaccacatga cgaaactgcg
      601 aaggccggga aaggtctatt tagtgggagc gggaccaggc gatcccgggc tcttgacgct
      661 gaaaggcaag gaatgtctgg agcaggctga tatcgtcctc tacgattatc tcgccaatcc
      721 ggtgttactg cagtatgctc cggccacagc ccaacgtatc tatgtcggtc ggaggggacg
      781 tgggcagtat caagatcaag ccgatatcaa tcgattgctc attgaacggg ccaaagaggg
      841 gaacgtcgtg gttcggctca aagggggcga cccgtttgtc ttcggacggg gtggagaaga
      901 ggcagaagcg gtgtctgccg gaggggtcga cttcgaggtg gtgcctggtg tgacggcagc
      961 ggtcgcggtt cctgcctatg cgggcattcc agtgacccat cggacgttgg cctccacggt
     1021 cacgtttgtc accggtcatg aagatcccac caagccggga acacagttgg aatggccgaa
     1081 gttggccagt gcatcaggga cgcttgtatt catgatgggc atgaagaatc ttccaacgat
     1141 caccagacag ttgctgtcgg aaggccggtc gcccgacacg ccggttgcag cgatccgttg
     1201 ggggaccaaa gccgatcaac agaccatcgt ggggactttg aatgatattg tcgaaaagac
     1261 ggaagctgcg catcttgaac cgccgacggc gatcgtcata ggtgaggttg tgcgattgcg
     1321 aggacagttg aattggtttg aagcaaaacc gctctttggg aggcggattg tgctcacccg
     1381 cccacagaag caggccagag agttttctca gctactgtcg ggctatggtg cggagccagt
     1441 tgaagtcccg acgattcaga tcgtgcctcc tgccagttgg caggctattg atgacgcggt
     1501 gactcgtctc gggacctatc attggctgat ctttaccagc gtaaacgggg tgaagccctt
     1561 catggagcgc cttcatgtgg cgcgaaagga tgtaagagcg ttagcgcacc tgcgtctgtg
     1621 cgcaattgga ccaagaacgg cgcaggaatt gggaacctat gggctgactt cggacgtggt
     1681 tcccacccag tatcaggcag agggagtgat tgccgcattg gctgatgcgg gagtccgggg
     1741 aagccacata ctaattccgc gcgccgaagt cgcacgcgag atcttgccgg aacagttgcg
     1801 ggagttaggg gcaaccgtcg atgtgattcc cgtctatcgt acgattgctc cggcggtgga
     1861 tgcgacgtcc ttgacgaaac agttttgcga cggtcaggtg gcggcagtga catttacgag
     1921 ttcctccacc gtccgaaatt ttgtggaagt gtttggaggg cgggacgcgg tgaaatccct
     1981 cgttgctcgg gtggtcattg cttgtattgg gcccattacg gcacgtacgg cggaagagta
     2041 tgggttgacg gtcacgatca tgccggctga gaataccgtg ccggcactcg cagaagcgat
     2101 cgtgaagcac tttaaggaag tggcgtgaat cgctgtttcg atgcgcggat taagctgagg
     2161 tagacggcag agtgacgaag taaattagca aagggaggac atactatggt ttcagtcaag
     2221 tcgttcatga ttccgaggga gaagtttgtg acggttgaac gggatatgaa tgtccagacg
     2281 gctgcccgta tcatgcggga tcgtggcatc ggcagcctgt ttgtgacgaa cgggaaggag
     2341 atcatcggca tcctcacgga tactgacatg gtccggcggg tggtcgcggc tggagctgac
     2401 acacagaaga caacggtcga acaggtgatg tccgcaccgc tcttggcgat cgacgagaat
     2461 aagtcattgt tagatgccaa cgatctgatg gccaaaacgc atactcgcca tctcggcgtg
     2521 actcaggatg gcagaatagt cggcatgatt tctgttcgtg acctcgtgct gttcttgacg
     2581 aatctcccga ggaagtgatc cgatggcatt cccaatacag cggctgcggc ggctgcggca
     2641 gcatgagtca tttcgtggca tggtacggga aacgcgcctt gccccgtccg acttgatcta
     2701 tccgctgttt gtcgtcgaag ggcgagatcg gcgggaggaa atcacctcaa tgccgggcca
     2761 gttcaggctc tcggtcgatc tcttagtgca agaggtcggc gagattgcgg ccttggggat
     2821 tcccgccatc atcctctttg ggattccaga ccggaaagat gatcggggga gtgcggggtt
     2881 cgatccgaat ggcatcgtgc aacgggctgt taaagccgta aagggtcagg ttcctgggct
     2941 gatcgttgtg accgacgtct gcatcgacga gtataccgat catggccatt gtggaatcgt
     3001 caaggacggc cgtattctca atgatgaaac gttggagtgc ctccgcgcga tggcgagaac
     3061 ccatgcgcaa gccggggctg acatggtggc gccgtccgat atgatggatg ggcgggtggc
     3121 agcgatccgc ggggaactgg atcaggccgg ttttccagag ctgccgatca tggcctatgc
     3181 cgccaagttc gcgtcttgct tttatgcacc gtttcgagaa gcggcgtgtt cgagcccgca
     3241 attcggtgat cggcaatcgt atcaaatgga tcccgccaat cgccgggaag cgcttcggga
     3301 gatcgctctt gatgtcgacg agggggcgga catcattatg gtgaaaccgg cactgccgta
     3361 tctcgacatc attgccgcgg ccagggagca ggtattgttg ccggtggcag cctatcaagt
     3421 gagtggggaa tacagcatga tcaaggcggc atccagagcc ggttggttgg atgaatcacg
     3481 agccatgatg gaatcgttgc tttcgattaa gcgtgccggt gcagatctca tcctgacata
     3541 ctttgccaaa gacgctgcgc gtctcctccg ctgacgacca tgctgaaagt gacgcgtggg
     3601 ctctccgacc aacgtccacg tgcctatccg gtcgccacga tcggcaattt cgacggccac
     3661 catctgggac atcgggcgct gttacagacg gtggtcgaga cagctcggaa ggcacaaggg
     3721 acatcactcg tgttgacctt cgaacctcat ccggtaagaa tccttgcccc acacgttgat
     3781 cttcactttc tcaccagtcc agaagagaag ctcgcccatc tcgaagtcac agggatcgat
     3841 gaagtcgtct tcttgggctt tactcccgcc tttgcggcaa tgagcccgga tcaatttgcc
     3901 gaagacatcc tgcatcgatc tctcggcata tcggaactat ttgtgggcaa tcattttgcc
     3961 tttgggaagg gtcgggcagg acgtatcgac gatctcgtgc gtctcggcgg acggtatggc
     4021 ttccaagtgc atc
//
LOCUS       DATIPF010000103         3259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_549180, whole genome shotgun sequence.
ACCESSION   DATIPF010000103 DATIPF010000000
VERSION     DATIPF010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549180"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VLL06_02585"
     CDS             complement(<1..224)
                     /locus_tag="VLL06_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29883.1"
                     /translation="MARKRFILGFILSLAFVFFSWPGPQTAQASCGSVTCFVVIGSQQ
                     QVSPKGLLTVNAFYNYTPQGTLLNGTDGVIP"
     gene            complement(344..649)
                     /locus_tag="VLL06_02590"
     CDS             complement(344..649)
                     /locus_tag="VLL06_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29884.1"
                     /translation="MNRTVLKFISILLVLCVLSVGGVAQAQMVEHAGHHAHHQAATHG
                     TVLCSWMCAAGTVLDTAVVPFQIELSPLAFITSSNSAQPSIEACQTSSSRAPPSFSL"
     gene            820..>3259
                     /gene="secA"
                     /locus_tag="VLL06_02595"
     CDS             820..>3259
                     /gene="secA"
                     /locus_tag="VLL06_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HSS29885.1"
                     /translation="MVTQLLNQIFGSKNDREIKAVRPIVERINSLESGLTPLSDHALA
                     DKSQEFKKRLEAGETLDDILPEAFAVCREMSRRKLNMRHFDVQLIGGVILHRGRIAEM
                     KTGEGKTLVATLPIYLNALEGKGVHLVTVNDYLAKRDAQWMSMLYHALGLSVGIIQHD
                     ASFFFDPTYEASDKRLQSLRPCTRPEAYRADITYGTNNEYGFDYLRDNLVVNDLAQCV
                     QRELNFAIVDEVDSILIDEARTPLIISGPTDETTDLYYRINAIIPQLKPERDYTIEEK
                     TKTASLTEDGNVRIEKLLGVDNLYDPNHMDLVHHVVKALQAYALYKRDVDYVVKEGEV
                     IIVDEFTGRLMPGRRWSDGLHQAVEAKEGVKIANENQTLASVTFQNYFRMYKKLSGMT
                     GTADTEAAEFAKIYNLDVNVVPTNRNMIRQDYADVVYRTEKEKFAAIVEDIKECYERG
                     QPVLVGTISIEKSERLAGFLSRTGVKHNVLNAKQHEREAEIIAQAGRKGAVTIATNMA
                     GRGTDILLGGNPDFMFKQILYREENLSDARKLEIFEEIRADCEKNKQEVLAAGGLHIL
                     GTERHESRRIDNQLRGRAGRQGDPGSSRFYLSLEDDLMRIFASERVSQLMLKLGMEEG
                     VPIEHGMVTRAIANAQKKVEAHHFDIRKQLLEYDDVMNKQREVIYQHRRAVLGGTNRK
                     DDLRDMMDTLVDSALNIYCPAEQYPEEWDIKGLAEIMHSQFGLDIAQDKSDGNSSIRD
                     MGRDALLEDLRAQVRDAYDRREQDLGSDLMRFLEKNFMLQVVDYHWKDHLLGMDHLRD
                     GIGLRGYGQKDPLIE"
BASE COUNT          811 a          895 c          922 g          631 t
ORIGIN      
        1 ggaatcaccc cgtccgtgcc gttcagcaag gtcccttgag gcgtatagtt ataaaaggcg
       61 ttgacggtga gcaaaccttt cggcgacacc tgctgttgag atccgatcac gacaaagcag
      121 gtgacggacc cgcacgatgc ctgtgcggtc tggggcccgg gccacgaaaa gaagacgaat
      181 gcaagactga gaatgaatcc gaggatgaac cgtttccttg ccatgatgct gctggccctt
      241 tctctctgga agaatcgttg ttccccgcct ctcctacaac ccactcagtg gattgtatga
      301 gcaacggatt gcggctctgc tgcgaaccga tgagcaacgg aagttagaga gagaaagacg
      361 gaggggcgcg gctggaagaa gtctggcatg cctcgatcga gggttgcgca gaattggagg
      421 atgtaatgaa tgcaagagga ctgagttcga tctgaaacgg gactaccgca gtatcgagta
      481 ccgttccggc ggcacacatc catgagcaga gcacggtgcc atgtgtcgcg gcctgatgat
      541 gggcatggtg cccggcatgc tcgaccattt gggcctgagc cactccgccg acggaaagga
      601 cacagagcac caagaggatc gaaataaatt tgagaacggt gcggttcatc tgcatttgca
      661 ttcggccaag tatgtatctc gaatcctaat ggacgtccac taggcctgtc aagccggcca
      721 cccaagggcc tttgcatatt ataggattca aagaagagat gttgtatact gccaaaatta
      781 cattaggaga tatcggcgtt cgttccggac gacagcatca tggtcacgca actactcaac
      841 cagatcttcg gtagcaaaaa cgatcgtgaa atcaaggcag ttcgcccgat tgtggagcgg
      901 atcaatagtc tggaatccgg cctgactcca ctttccgatc acgccctcgc ggacaagtcc
      961 caggaattca agaagcgcct ggaggcaggg gaaaccctgg atgacattct gcctgaggcc
     1021 ttcgcggtct gtcgtgaaat gtcccgccgc aagctgaaca tgcgccattt cgatgtgcag
     1081 ctgatcggcg gcgtgattct ccacagaggc cgcatcgcgg aaatgaagac cggcgaaggg
     1141 aaaaccctgg tggccacgct gccgatctat ctcaatgcgc tcgaagggaa aggcgtccac
     1201 ctcgtcaccg tgaacgacta tctcgccaag cgcgatgcgc agtggatgag catgctctat
     1261 cacgcactgg ggctttcggt aggtatcatc cagcatgatg cctccttttt ctttgatccg
     1321 acctatgagg catccgacaa gcggcttcag tctctccgcc cctgcacgag gcccgaagcc
     1381 tatcgggcgg atatcaccta tgggacaaac aacgagtatg ggttcgatta tctccgcgac
     1441 aacctggtcg tcaacgattt ggcccagtgt gtacagcgcg agctgaactt cgccatcgtg
     1501 gacgaagtcg acagtatcct catcgacgag gctcgtacac cgttgattat ctccggtccg
     1561 acggatgaaa cgaccgacct ctactaccgc atcaacgcca ttattccgca gctgaaaccg
     1621 gagcgcgact acacgatcga ggaaaaaacg aagaccgcgt cgttgaccga agacggcaac
     1681 gtccggatcg agaaattact cggcgtggac aacttgtatg accccaacca tatggatctc
     1741 gtccaccacg tcgtcaaggc gctccaggcc tatgccctct acaaacggga tgtggactat
     1801 gtcgtgaaag agggggaagt catcatcgtg gatgaattca ccgggcggct tatgccggga
     1861 cgtcgatgga gcgacgggct gcaccaggcc gtcgaagcca aagaaggtgt caagattgcc
     1921 aatgagaatc aaacgttggc ctcggtcacc ttccagaact atttccggat gtacaagaaa
     1981 ctcagtggca tgactggcac ggcggatacc gaagcggccg aattcgccaa gatctataat
     2041 ctcgacgtca acgtggttcc gaccaatcgc aacatgatcc ggcaggatta cgcggacgtc
     2101 gtctaccgaa cagaaaaaga gaagttcgcc gcgatcgtcg aggacatcaa ggaatgctac
     2161 gagcgcggac agcccgtctt agtcggcacg atttccattg aaaaatcgga gcgactcgcc
     2221 ggattcttga gccggaccgg cgtgaaacac aacgtcctca acgccaaaca gcatgagcga
     2281 gaagcagaga tcattgccca ggccggacgc aagggcgccg tcaccatcgc cacgaatatg
     2341 gcgggccgcg gcacagacat tctcctaggc ggcaatcccg actttatgtt caagcagatc
     2401 ctctaccgcg aagagaatct gtctgatgca cgcaagctgg agatctttga ggaaattcgg
     2461 gccgactgcg agaagaacaa gcaagaagtt ctcgcggcgg gcggacttca cattctcggg
     2521 accgagcgcc acgaaagccg ccggatcgac aaccagcttc gaggccgagc cggtcgccag
     2581 ggcgaccctg gttcatcacg attctacctg tcgttggaag acgacctgat gcgtattttc
     2641 gcctccgagc gggtctctca actgatgctc aagctcggca tggaagaggg tgtcccgatc
     2701 gagcacggca tggtcacccg ggccattgct aacgcccaga agaaggttga agctcatcac
     2761 ttcgacatcc ggaaacagct gctggagtat gacgacgtca tgaacaagca gcgtgaggtg
     2821 atctaccaac accgacgcgc cgtcctgggc gggacgaatc gaaaagacga ccttcgcgac
     2881 atgatggata cgctggtgga ctccgcgctg aacatctatt gcccagctga gcaatacccc
     2941 gaagaatggg atatcaaagg attggctgaa atcatgcata gtcagttcgg tctcgatatc
     3001 gcccaagata aaagcgacgg caactcgtcc atccgcgaca tgggtcgcga cgcgttgctc
     3061 gaagaccttc gtgcgcaggt gcgcgacgcc tatgatcgga gagagcagga cctgggctcg
     3121 gacttgatgc gcttcctgga gaagaacttc atgctccagg tcgtcgacta ccattggaaa
     3181 gaccatctcc tggggatgga tcacctccgc gacggaatcg ggttgcgcgg ctatgggcaa
     3241 aaagatcctc tcattgagt
//
LOCUS       DATIPF010000104         6008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_372242, whole genome shotgun sequence.
ACCESSION   DATIPF010000104 DATIPF010000000
VERSION     DATIPF010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372242"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            358..1317
                     /locus_tag="VLL06_02600"
     CDS             358..1317
                     /locus_tag="VLL06_02600"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS29886.1"
                     /translation="MLRTWAAQGNALAQNNLGLLYDLGDGVPRDYAVARQWYEQAAAQ
                     NYAAAQFNLGLLYANGHGVPQDYAMAREWYEKAAAQGRANASYILGEMYAHGQGVPQD
                     DAKARQWYEKAAAQGSADARGWFEKAAAQGNTHAQFTLGSLYETGGGVPRNYGEATKW
                     YEKAAVQGDTDAQFSLGLRYAQGYSVPQDYAKARQWYEKAAAQGNAGAQYKLGQLYHF
                     ALGVPQNYAEATKWYEKAAAQGDADAQFSLGLMYATGDGVSQDDVRAYLWYTLAVAHS
                     TGNKQKSAAANRDGALTRRMTPAQVVEAQRLAQQCEAQHFKGC"
     gene            1491..2060
                     /locus_tag="VLL06_02605"
     CDS             1491..2060
                     /locus_tag="VLL06_02605"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HSS29887.1"
                     /translation="MKTNVRLLLGLCGTLWIGMLTGCTGDRSHVELQRMKQLLAEIEQ
                     ARQMEQANLANFDDLDFNVYSGQKWDQLGKSHAQDITVYWPDGRTTKGIDAHIDDLKV
                     MFVFAPDTHIKEHPIRIVSGDWTAVTGYIEGTFTQPMPIGAGQTIPPTGKSFKLYMVT
                     IGHWKNGVMVEEWLIWDNLAFMKQIGLAQ"
     gene            complement(2212..2583)
                     /locus_tag="VLL06_02610"
     CDS             complement(2212..2583)
                     /locus_tag="VLL06_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS29888.1"
                     /translation="MTKILVIDDDARDRGLLVAVLEERGYEVILADTGGTGLMLCHRQ
                     TPDAVVLDLNMPGIDGRSILQQLRILHPTLPVVVFSGHGTEEVEQEMLNQGATAFIQK
                     AFSLDRLGLALQEVLPSPHTS"
     gene            complement(3177..4574)
                     /gene="lepB"
                     /locus_tag="VLL06_02615"
     CDS             complement(3177..4574)
                     /gene="lepB"
                     /locus_tag="VLL06_02615"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HSS29889.1"
                     /translation="MMRLIFWLKVVVLAAGFGTALGLTPYTVGLVQGFSPELDTEDLK
                     ALAVLIVIPSLIVWTSLAEGLPNARLTTAIACGLAWPFVTLLIGVYLNRTFHYDLAHL
                     QFHLIFGLWAFSPLLGTTVWACVLLLGATAGVLMHRVVNTQDAKVPCETPQAQGATQD
                     QHTAASNPVRSLPWVAFSLSLLPGAGQLYNREYWKAGLLFVLGMGGGTVFFRFLLLHG
                     RFDAPYNVLVATGLLLTVLLGIMTEAFLMARHCEQNFWGFSKPRYYVFIAIFFVMGFA
                     VKPVMLGPIDRSFATYSMIMNPAMEPTILSGDRFLVDRTAYTRSHPPHRGDIIMFQSP
                     HHGPLIRRIVGVPGDTIEIRNHVVLINGRRPDEHGARYPHVFGIKNDSHDSQANFGPV
                     VVPEGDVFVLVDNRESDWDSRVFGTIPMAKIWGRIAVICWSWDGNSFLPQWERIGIRF
                     PSGSNSQPSGDSPLF"
     gene            complement(4842..5459)
                     /locus_tag="VLL06_02620"
     CDS             complement(4842..5459)
                     /locus_tag="VLL06_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29890.1"
                     /translation="MIIGFLFIPLWIPTLTEMAKIAIGTEDYRYTVALSDDGNMLRVD
                     GGITTGLTRTITEQLSRHPQVELIVLNSHIGLLEEGWKLQTLIEQRGLSTYTDRQCEG
                     ICTTVFLAGAQRMLQADAHLGFHKVRIAGVPDSLNQTENNDVRDYMLKQGIQQGFVDA
                     AVDIPSDEVWIPSTDELIEAGVVTEIVDGNQAIVRPQLLSTRGGQ"
BASE COUNT         1441 a         1592 c         1691 g         1284 t
ORIGIN      
        1 aagattcatg tgtttttggt cctgatgacg agtacgcctc aatcactgaa aaatcaagaa
       61 ggttgttggt cgtttcgaag caggattgtg acccatcaac gcacgcttcc attttttctc
      121 atcctcaaag gaaggcccat accatgctcg caggagacac gccgatactc ttcgcaagcg
      181 accgcagcga ccagcacttg tctccctcat tgtgtcattg acggtccctg ggctccgacc
      241 ccatactgcg ttcccatgcg caccctctct gtcatcacac ttttggctgc attggttttt
      301 ccatctgggt atgcgtcagc ttccccaccg atcaacattt cagcagaaga actgcacatg
      361 ctacgcacat gggcagccca aggcaacgca ttggcgcaga acaacctcgg actactgtat
      421 gacctcgggg atggcgtgcc ccgggattac gcggtcgcgc ggcagtggta cgagcaagcc
      481 gctgcccaga attacgcggc agcacagttc aatctcggac tgctctatgc caatgggcat
      541 ggggtgcctc aggattacgc aatggcgcga gagtggtacg aaaaagccgc cgcacagggc
      601 agagcgaatg cgtcctacat ccttggtgaa atgtatgccc atggacaggg cgtcccgcag
      661 gatgatgcaa aggcacgaca gtggtatgag aaagccgcag cccagggcag cgcggatgcg
      721 cggggatggt tcgagaaagc ggcggctcaa gggaacacgc atgcgcagtt caccctcggt
      781 tcgctgtatg aaacgggagg cggcgtcccg cggaattacg gggaagcgac gaagtggtac
      841 gagaaagcgg cagtgcaggg ggacacagat gcgcagttca gcctcggatt gcgatatgcc
      901 cagggatata gcgtacccca ggattatgcg aaggcgcgac aatggtatga gaaagctgct
      961 gcccagggta acgcgggagc gcagtacaag ctcgggcagc tgtatcactt cgcgctcggc
     1021 gtgccacaga attacgcgga agcgacgaag tggtacgaga aagcggcagc gcagggggac
     1081 gcggatgcac agttcagcct cgggctcatg tatgccactg gagacggcgt gtcgcaggac
     1141 gatgtgcggg catatttgtg gtacaccctc gcagtcgccc actcgacggg taacaagcag
     1201 aagtccgcag cagccaatcg agatggggca ctcacacgcc gcatgactcc cgcacaggtt
     1261 gtagaagccc agcgactcgc gcaacagtgt gaagcacagc atttcaaagg ctgctgaaaa
     1321 gggtccgtcg aagtcccatc tttatcagaa cgcccacgcc actagacttg gcctgggacg
     1381 tggttcagcc cgttccccgc gacgcagcca tcagtatagg caagagtcga gtgtcatatt
     1441 tgccacgatg catggatacg ggttctctaa cacataagga gggatcagcc atgaagacga
     1501 acgtccggtt gctccttgga ctctgcggga ctctctggat cggaatgctg acgggttgta
     1561 ccggcgatcg aagtcatgtc gaactccaga ggatgaagca gttgctcgct gagattgaac
     1621 aggcgaggca gatggagcag gcgaatcttg ctaacttcga tgatcttgat ttcaacgtct
     1681 atagcggcca gaaatgggat caactcggca agagccacgc gcaggacatc acggtgtatt
     1741 ggccggatgg ccggacgacc aaaggcatcg acgcgcatat tgacgacctg aaggtcatgt
     1801 ttgtcttcgc gccagatacg catatcaagg agcatcccat cagaatcgtc tcgggtgact
     1861 ggaccgcagt caccgggtat atcgagggaa cattcacaca gccgatgcca attggcgcgg
     1921 gccaaaccat tccaccgacc ggcaaatcgt tcaagctata tatggtgacc atcggtcact
     1981 ggaagaacgg cgtgatggtc gaggaatggc tgatttggga caacctcgcc ttcatgaagc
     2041 aaatcggcct ggcccaatga gtgcgccgag tagctgggca tgaacaccgt accggctcct
     2101 cggatgcctg tcgtgttcat cgggcatggc aatccgatga atgcgattga acacaacgaa
     2161 ttccatcgca gtcggacagt cagtcctgat gggagtctca gctgggagcc ttcaacttgt
     2221 gtggggggag ggtagaacct cctgcaatgc caacccgagt cgatccagcg aaaatgcctt
     2281 ctgaataaag gccgtggcgc cctggttcag catctcttgc tcaacctctt ctgtgccgtg
     2341 tccactaaac actacgaccg gtagggtggg gtgtagtatc cgcaattgct gcagtatgct
     2401 gcgaccgtct attccaggca tgttgaggtc caacacgaca gcatcaggag tttgtcgatg
     2461 gcataacatc aaccctgtcc caccagtgtc tgccaaaatg acttcatatc ctctttcttc
     2521 taggacggca actaggaggc cccggtcgcg ggcatcatcg tctatgacga gaatcttcgt
     2581 catgtgagcc ttcatccgct atttaacctg ccgccccgac tcatccctac accgtagatg
     2641 aactcagcag cgcagatggc acggtcacga tcagtaaccc atcatgtcag tggaggcagc
     2701 gacgatccag cattttttgc tttcatcatc tgcatctgct acgggatcct gcgttgtggt
     2761 ttcttgtggg gttagttagt ggtctgaggt tgggttatcg gaaattcgtg aaccggaggt
     2821 caccgctggt cccactctag ggcactcgaa ttccacggtt cgcaaagggg acaattcggg
     2881 gagccatcaa atttgcactg ctttccagtg caggttctac catgaagccg tcagtcatca
     2941 tttctgatgc tggagatgga tggtgcattc cagttcattc gggcctattt cagccaaagt
     3001 tccaattttg aggaacccta ggctggagtg ggccccagtg agttccggta ggcctgagta
     3061 ggatcgtaat tgcggtcttc taaacggctg gtcaaaaacc aaaggtcccg aaaccgtcca
     3121 ggcgagctga ggactaacat gaaaggtgat acacgaactc tcagatggtt accttttcag
     3181 aaaagtggag aatctcctga aggctggctg ttactgccgg agggaaatcg gattccaatc
     3241 cgctcccatt gtggcagaaa ggagttgcca tcccacgacc aacagatgac cgcaatcctt
     3301 ccccaaatct ttgccatcgg aatcgtgcca aacacgcgac tgtcccagtc gctctcccga
     3361 ttatctacca agacaaacac gtctccctct ggaacgacga cgggcccaaa gttggcctgt
     3421 gaatcatgtg agtcattctt gatcccgaag acgtgaggat acctagcgcc gtgctcgtct
     3481 ggacgtctcc cattgataag gaccacgtga ttgcgaattt cgatggtgtc gcctggcact
     3541 ccgacaattc gtcggatgag tgggccgtgg tgcggtgact ggaacatgat gatatctcca
     3601 cggtgcgggg gatgcgagcg ggtgtacgcg gtccggtcta ccagaaatct gtccccggag
     3661 aggatcgtgg gctccatggc aggattcatg atcatagaat aggtggcgaa cgaacggtca
     3721 atggggccca gcatcaccgg ttttacggca aaccccatca caaagaagat ggcgatgaat
     3781 acatagtagc gaggttttga gaagccccag aagttctgct cacagtgccg ggccatgaga
     3841 aatgcctccg tcatgatccc gaggagaact gtgagcagga gccccgtcgc gacgagcacg
     3901 ttgtaggggg cgtcgaaccg accgtgcaag agtaggaagc gaaagaacac ggttcctcct
     3961 cccattccca gcacaaacaa caacccggct ttccagtact ccctgttgta cagttgtccc
     4021 gctccgggaa ggaggctcag agaaaatgcc acccagggga gcgagcggac agggttgcta
     4081 gctgcggtgt gctgatcctg cgtggcaccc tgggcctgag gggtttcgca aggcaccttc
     4141 gcatcctgcg tgttgaccac ccgatgcatg agcacaccag ctgtagcccc taagagaagg
     4201 acacatgccc acaccgtagt tcctaagaga ggggaaaatg cccacagccc aaagataaga
     4261 tggaactgga ggtgggcgag atcgtaatgg aatgtccgat tgagatagac cccgatcaga
     4321 agcgtcacga acggccaggc gagtccacag gcaatggcgg tggtcaagcg tgcgttgggg
     4381 agcccttcag ccagggaagt ccaaacaatt agggagggaa tcacaattaa tacggcgagc
     4441 gctttgagat cctcggtgtc cagttccgga gagaatccct ggacgagtcc cacagtatag
     4501 ggggtgaggc ccaacgctgt gccaaacccc gccgcgagaa cgacgacttt gagccagaag
     4561 attaatcgca tcattctgcc aaatatctga attagacgcg cgcgcgaact ttctccattc
     4621 ttcaagagac ggtgttgctg tccgtcagaa ctcgccgaac agtcggacct gctttatccc
     4681 tcacatgcct ctccgacagt tcatgtgtca cgaaacagaa attgtccgtg ctctgggtcg
     4741 aaaggaaatt ggaacgtcat gttagacaag tcgtgcgaga ggatacggta attcgaaatg
     4801 ccggctagaa agctcacgat cgttggggcg gcattccgtc ctcattgccc acctctggtg
     4861 ctcaagagct gtggacgtac aatggcctgg ttgccatcaa caatttcagt gaccacacct
     4921 gcctcgatga gttcatcggt actggggatc cacacttcgt ctgagggaat atccacagcc
     4981 gcgtcgacaa acccttgttg gataccttgc ttaagcatgt agtctctgac gtcattattt
     5041 tcagtctgat ttaacgagtc tggaacacca gcgatcctca ctttgtgaaa tccaaggtga
     5101 gcatcggctt gcagcatccg ctgcgcgcct gccaggaata cggttgtaca gatgccctcg
     5161 cactgtcgat ccgtataggt tgataaacct cgttgctcaa tcaaggtttg aagtttccag
     5221 ccttcttcta ggaggccaat atgggaattg aggacgatga gttccacctg cggatggcga
     5281 ctcagctgtt cggttatcgt ccgagtaagc ccagtggtga tgccgccatc cacacgcagc
     5341 atattgccgt catccgaaag ggccaccgtg taccgatagt cttccgtgcc aatagcgatc
     5401 tttgccattt cagtaagggt gggaatccag agaggaataa agaggaaacc aatgatcacg
     5461 attccctggg cacatcgccc ccaaataggg ctggacgtgt tggctacgta ctgacgagcg
     5521 gctcgccaca accctaccaa ttgccatggt cccagaaggc atatacacaa gaatgtaaac
     5581 acggttgaga gttggtggat tcgcaaaggg gacgagctgg tctcttgccg attctctaag
     5641 aacgtgtcta tcaaggtcgt cacgaggtcg agaccacagt tggtgagcca aaaagaatgg
     5701 gcgagggaaa gttcgcctct ccaatatctg gcgaagtaat gtgtagctgg ttttctgttg
     5761 tgagacaccg atactcgagg agtgacctgc cgcacgggag ttgacgtggg tgacggatcg
     5821 tcgccagttc cctctttgcg ataggcgtcc aacggatggc cacatttaca gtgcatgacc
     5881 gtcacgggat tccggttctg acatttggaa cagattttgt gtaataccat tcttttaatc
     5941 cctcggcacg ctgtggctac aaagtcatcg ggagcaatac cttcggtaga gaatctatag
     6001 gtttacaa
//
LOCUS       DATIPF010000105         6073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1344003, whole genome shotgun sequence.
ACCESSION   DATIPF010000105 DATIPF010000000
VERSION     DATIPF010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344003"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /locus_tag="VLL06_02625"
     CDS             complement(<1..1462)
                     /locus_tag="VLL06_02625"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HSS29891.1"
                     /translation="MTINGLVQIGLCIVVLLALVRPLGWYMARVYEGHPCGLDRVMGP
                     LERLIYRLCGVRQSEEMDWKTYGIAMLLFNAMGLVALYAMQRLQGFLPLNPGGLGAVT
                     TDLAFNTAASFATNTNWQAYGGETTLSYLTQMLGLTVQNFLSAATGMAILVALIRGLT
                     RRSATTIGNFWTDLVRSTLYILLPLAFLFSLLLVSQGVVQTFASYQTAPMLQPVSYAK
                     PVMDANGQPVLDEQGQPKTEPATAAEQLLAVGPAASQIAIKQLGTNGGGFFNVNSAHP
                     FENPTPWSNFLQVLAILLIPAALCYTFGMMVGDTRQGWVLLAAMTILLLCFIPLGLWA
                     EQHGNPAFTALGIDQASSAGQSGGNMEGKETRFGIANSVLWSAATTAASNGSVNSMHD
                     SYTPLGGLVPLFLIQFGEVVFGGVGSGLYGMIAFAIIAVFVAGLMVGRTPEYLGKKIE
                     PYEMKMASLLILIMPMLVLGFTAVASVTGVGTSSIFN"
     gene            complement(1462..1551)
                     /gene="kdpF"
                     /locus_tag="VLL06_02630"
     CDS             complement(1462..1551)
                     /gene="kdpF"
                     /locus_tag="VLL06_02630"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HSS29892.1"
                     /translation="MDAMYLLGGILSLGLLVYLMVALLKPEWF"
     gene            complement(2044..2169)
                     /locus_tag="VLL06_02635"
     CDS             complement(2044..2169)
                     /locus_tag="VLL06_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29893.1"
                     /translation="MNTGWEVRPLGWVLLALLVGVMIYYLAIKRTRPSQDDHPTR"
     gene            complement(2395..2598)
                     /locus_tag="VLL06_02640"
     CDS             complement(2395..2598)
                     /locus_tag="VLL06_02640"
                     /inference="COORDINATES: protein motif:HMM:NF019826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HSS29894.1"
                     /translation="MKTNKHQTLLLVKTKKAVRAKDLVQAFGYSAPTARSYLSHLKRQ
                     ELLERISAGHALTEKGRTRLQFF"
     gene            complement(3047..4282)
                     /locus_tag="VLL06_02645"
     CDS             complement(3047..4282)
                     /locus_tag="VLL06_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HSS29895.1"
                     /translation="MSPADNPPPRVPSPLESLLKKGDYTFIPLPAFGYNRNEGYWLGS
                     QMWIFKEDARGHLGTLIVPQYFYNRLVGQTINGNYFRYPSSTAQYEITGSISEHVARD
                     IELRYKDVGAGGGRYIIMGQAQWFKNPLDRFFGLGNRAQELQETNYTSEEIKVTLTGG
                     VKLTPDFAVLFTERFRNVNVETGIVDSLPSTLQRFGQVAGIEGAKIIGHRLTLRYDTR
                     DHQLSPTKGTFITASGEFDQNLQHEEDNRWLRYTIDARTLIPHAQGQMIFVFRFLADG
                     VAGVKSETLPTGQTEETQRGIPFYERPMLGGETTLRAFGQNRFISNSALLFNFEERIL
                     LTEREIFGYDIAMEIAPFLDLGRVERVNLWQRLDFKNWQVNPGTGFRLMAKPYVVGRA
                     DFAYGRDGFNAFIGLDYPF"
     gene            complement(4595..5062)
                     /locus_tag="VLL06_02650"
     CDS             complement(4595..5062)
                     /locus_tag="VLL06_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HSS29896.1"
                     /translation="MKKCKRQLQGGAAVWLLGLTLFVLLPGCSVLLIHEAADTDSQDV
                     VALLKEGADANAPFPIVGTRALMVAASHGNIDTVRALLDAGADVNAKDFTGWTALHAA
                     AFKGDLEIVQLLLEHGAIPGKGHWYIRSPLGMAEGLGHTEIVPLLKEAEARAQ"
     gene            complement(5186..5713)
                     /locus_tag="VLL06_02655"
     CDS             complement(5186..5713)
                     /locus_tag="VLL06_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29897.1"
                     /translation="MRFRHSIIPAVTVLLMSIGLFCGAEAKTIEAGHATVSWKGKGVI
                     DDLGNGDKVFSGTLTGMILVKHLPEGSEPAQIHKTKMDCQTILYINKNVEERKTILCI
                     LRAHEDRDVAYGEIRCVGKTGECKGEFTWVWGKGGFKGITGTTPFVGSIYIEEQKEGE
                     IYGSAHWPELTYTLP"
BASE COUNT         1429 a         1659 c         1644 g         1341 t
ORIGIN      
        1 ggttgaaaat cgagctggtg ccgacgcccg tcaccgacgc cactgccgtg aaaccgagaa
       61 ccaacatcgg catgataaga attaacaggg aggccatctt catctcatag ggctcaattt
      121 ttttcccaag atattcgggt gtcctcccca ccatgagtcc ggccacgaac accgcaatga
      181 tggcgaaggc gatcatgccg tagagacccg agccaacccc accgaagacc acttctccga
      241 actgtatcaa gaagagcggc accagccctc ccaagggtgt gtaggaatcg tgcatcgaat
      301 tgaccgagcc gttcgaggct gccgtggtgg cggcagacca cagaacggag ttcgcgatgc
      361 cgaatcgcgt ctctttccct tccatgttcc cgccggattg tccggcactg gaagcctgat
      421 cgatgcctaa ggcggtaaaa gccggattcc cgtgttgctc ggcccatagc cccagcggaa
      481 tgaagcagag caggagaatc gtcatcgcgg caagcagcac ccagccctga cgtgtatctc
      541 cgaccatcat cccaaacgtg taacagagcg ccgccgggat aagcaggatc gctaatacct
      601 gtaaaaaatt ggaccagggc gtcggatttt cgaacggatg cgcggaattg acattgaaga
      661 atccgccgcc gttggtcccg agctgcttga tcgcgatctg agaggctgcc ggtccgaccg
      721 caaggagctg ttcggctgcc gttgccggct ctgttttcgg ctggccttgc tcatccaaca
      781 ccggctggcc gttcgcatcc atgacgggct tggcgtaact gaccggctgg agcatgggag
      841 cggtctggta tgacgcgaac gtctggacga ccccttgtga caccaagagc agcgaaaaga
      901 gaaaggcgag cggcaggagt atgtacagcg tgctgcggac gaggtccgtc caaaaatttc
      961 cgatagtcgt ggcgctgcgc cgggtcagac cccggatcaa cgcgaccagg atcgccatcc
     1021 cggtggctgc ggacaaaaaa ttttgcaccg tgagtcccag catctgcgtc agatagctca
     1081 gcgtcgtttc gcctccgtaa gcctgccaat tcgtattggt cgcgaagctc gccgcggtgt
     1141 tgaaggcgag atcggtcgtg acggctccca acccccctgg attcagcggc agaaatcctt
     1201 ggagcctctg catcgcatag agcgcgacca gtcccatcgc gttgaataac agcatggcga
     1261 tcccgtacgt tttccaatcc atctcctcgc tttgacgcac tccgcagagg cgatagatca
     1321 gccgctcaag aggacccatc actcggtcga gcccgcacgg gtgaccttca tacactcgtg
     1381 ccatatacca gcccagcggc ctgaccaatg cgagtagcac gacaatgcag agtccgattt
     1441 gaaccagtcc attgatcgtc atcagaacca ctcgggtttc agcaacgcga ccatgagata
     1501 caccaacaat cccaatgaca gaattccacc gagcaggtac atcgcatcca tggcgaaatc
     1561 ccctttacag tcgctccagc accgagatga gccaaccgga cagtagaaaa aatatcgcta
     1621 cgatagccag atagatgaga tccatggcgc atacctctca atcaacaaca atggaaatct
     1681 tgtccatatg tctttgtccg aattcatact ggaccgtaag aagtaaaaat ggtgtaaaaa
     1741 ccgtggagca tctcgaacag ttttcttgtg atttgatgcg acggtgtcgg actggatgtt
     1801 gcgacgaccc gcaccgggca aattgtcttt ctcgccgagt aaactagcca ttccgagtgc
     1861 agcgagtaag gccgcgtatg acgcgtaagc tagctgtatg tgatgtatgc gttcctggta
     1921 cgagccagcc tcacgtgtaa aaaaaccatg ctcggtcatg accagggagt agcgggaaaa
     1981 ccgatgtgga gtgaggctga aacgcgtgcg aatgatgggc caacggagag gctgaagaaa
     2041 gagttaccgt gtcgggtgat cgtcttgaga gggacgtgtg cgcttgatgg cgagataata
     2101 gatcatcacg ccgacgagaa gggccaggag tacccagccc agtggacgga cttcccatcc
     2161 ggtattcatg cggcctcctt gggaatgccc atcaggtgcg cctgcgtttc actgaggaga
     2221 gacttccagc atcggggaca gtagaccccg gcatgacgga ccaccagcag gaaatcctgt
     2281 gtgggaagaa tgcgtgcttg tgcgtggggc atctgctagg tgcatcgggg acaggtcatg
     2341 aagccggtct tgcgttgaca aagcggacag gcaggatcgg cgcatccact cacatcaaaa
     2401 aaactggagt cgggtccggc ccttctcagt gagtgcatgc cctgcactga tccgttccag
     2461 caattcctgc cgcttgagat gggaaaggta cgaacgagcc gtgggcgcag aatagccaaa
     2521 ggcctgcacc agatccttgg cccgtaccgc cttcttggtc ttcacgagca gcagcgtttg
     2581 atgcttattg gtcttcaagg tgcaattccg aggccgtgag atctccgaaa aggatgacac
     2641 gagattcaat cagattacag ttgctcgggt gcgtaggacg ctgaccgagc gtatctaaac
     2701 cggttcacgg gtgaatctaa aatggtaaac aaacctgaaa caaaggtgaa cgggcgtggg
     2761 gtcaggaaac atttgttttt ggaacaggcg gaaggatacg tcaaagccct tgaaaaatcg
     2821 acttctaggg tcttgagtta aagagaggtt tttggacact gtgtgcagac aggactcgaa
     2881 tgagatagaa gttacggtcg taaaacggtc gtatagcagg gctattttcc tctcccttcc
     2941 gctcactcaa ccagcggtgg ccactgaatc gggagaagac caagagtggg ttggaatcgt
     3001 ggaatgagat ggagttgagt acaatcggct gtcagggcat gcagtgtcaa aacgggtaat
     3061 ccagtcctat aaatgcgttg aatccatccc gtccataggc aaagtcggca cggccgacga
     3121 catacggctt ggccatcaac cggaagcccg tacccgggtt cacctgccag ttcttgaaat
     3181 caagcctctg ccatagattg acccgttcca cgcgccccag atcgagaaag ggcgcgattt
     3241 ccatcgcgat atcatagccg aaaatctcac gctcggttaa caggatgcgc tcctcgaagt
     3301 tgaagagaag ggcggaattg ctgatgaagc ggttctggcc gaatgcgcgg agggtcgtct
     3361 cgccacccag catcgggcgc tcataaaatg gaatgccacg ttgtgtttct tcggtctggc
     3421 ccgtcggcag ggtttccgat ttcacaccgg ccacgccatc cgctaggaat cggaacacga
     3481 aaatcatctg gccctgggca tggggaatca acgttcgggc atcgatggta tagcgcaacc
     3541 agcggttgtc ttcctcatgt tggagatttt ggtcgaactc cccgctcgcc gtgataaagg
     3601 tgcctttcgt ggggctcagt tgatgatcgc gagtgtcata gcgcagggtc aggcgatgtc
     3661 cgatgatctt cgccccttca atgcccgcga cttgcccgaa ccgttgtagg gtcgatggca
     3721 gggaatccac aatacctgtt tccacattca catttcgaaa ccgctccgtg aagagcacgg
     3781 caaagtcggg agtgagtttg acgcctccgg tgagggtgac cttgatttcc tctgatgtgt
     3841 agttggtctc ctgaagttcc tgggctcggt tccccaggcc gaaaaaccgg tccaacggat
     3901 tcttaaacca ctgggcttgc cccataatga tatagcggcc accgcccgcc ccgacatcct
     3961 tgtaccgaag ctcaatatcg cgcgcaacgt gctcagaaat cgatcctgtg atctcatact
     4021 gagccgtgct ggaggggtag cggaaataat tgccgttgat cgtttgccca accaggcgat
     4081 tatagaaata ctgcggtacg atgagtgtcc ccaggtgccc gcgcgcatct tctttgaaga
     4141 tccacatctg actaccaagc cagtaccctt cgttccggtt atagccgaac gccggaagcg
     4201 ggataaatgt atagtcgccc ttcttaagta atgactccaa cggactcggc acccgaggtg
     4261 gtgggttatc tgctggtgac actgtttgct ccgctgtctg tccgctctcg tgaactgcct
     4321 gagcgcgggc ctttcctagt agcggtgtgg ctaccgtcaa cacgcagacc agtaggcaga
     4381 gagatgtccc gacgactagg gtatgcgtca atctgacctg cgtgacaata ggttgaagaa
     4441 agtgggcacc gttgtgatga cagagacgcg gtctagaaag ggccggttca caatcgtcag
     4501 catgcggaca gcgttacgac aacttcatcg cgatgtcttt ggagtacccg ttgtgtcaca
     4561 cgcagttgat ttttgaacct tctacactcg ggaatcactg ggcgcgagcc tcagcttcct
     4621 tgagaagcgg cacgatctct gtatggccga gcccttccgc catccccaaa gggctacgga
     4681 tataccagtg tcctttgcct ggtatcgcgc catgctccaa cagcagttgg acgatttcaa
     4741 ggtcaccttt aaaggctgct gcgtgtaagg cagtccaccc tgtgaagtct ttcgcattca
     4801 catcggcccc ggcatcgagc agcgctcgta cggtatctat attgccatgt gatgcggcca
     4861 ccatgagcgc gcgagtccca acaattggaa atggggcatt ggcatctgcg ccctccttca
     4921 acagagccac cacgtcttgc gaatccgtgt cggctgcctc atggatcagc aggacgctac
     4981 atcctggcag caagacaaat agggtaaggc ccaagagcca caccgcagcg ccaccttgca
     5041 gttgcctttt acatttcttc acaaatcaac accatgtgtt tttgaacccc atcggctcca
     5101 aaaaggactg gttctgaaga ttacctcccc catgagtatg gactggtagt cgtgctgtcc
     5161 ttcttaccgg gagaggctca aacccctatg gcaacgtata ggtcaactcc ggccaatgtg
     5221 cacttccgta gatctctccc tccttctgct cttcaatata gatactgccg acgaacggcg
     5281 tcgtccctgt aatacccttg aatccgccct ttccccaaac ccatgtaaac tcgcctttgc
     5341 attcgcctgt ctttcccaca cagcgtattt ctccatatgc gacgtccctg tcttcatgag
     5401 ctctcaagat gcacaggata gtctttcgtt cctctacatt cttattgata tagagaatcg
     5461 tctgacaatc cattttggtt ttatggatct gcgccggttc cgagccttcg ggaagatgct
     5521 tcacaaggat catcccggta agagtgccgc taaagacttt gtccccattc cctagatcat
     5581 cgatcacgcc ttttcctttc cagcttacag tcgcatggcc cgcttcgatg gttttggcct
     5641 cggctccgca gaagagtcct atgctcatga ggaggacggt gaccgccggt ataatggaat
     5701 gcctgaatcg catgattact cccttcatat atatacgacg ctgctttccc gattgaggga
     5761 gaaatagcgc gctggtgagt gaatatccat ttgccaaacc gtgacttaac ggtctatgtg
     5821 cgcatgctct ttggaactat gtcatgaatc tcatagatgt atttttgaac cgtcggaacc
     5881 atacgcgaac ttctagcaaa aatcgaggtg gtagttgcaa tgacgctcgc ctactcagtt
     5941 gaaagaggga cggagaaata gggcaggcta ctttgcggaa agcagactgt caacgaatgg
     6001 attggtgaca tgcacgtatt tcaggaagca ggcaggatcg gctcgaacac gactcagacg
     6061 cggtgagcgg cgg
//
LOCUS       DATIPF010000106         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_637341, whole genome shotgun sequence.
ACCESSION   DATIPF010000106 DATIPF010000000
VERSION     DATIPF010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637341"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1834
                     /locus_tag="VLL06_02660"
     CDS             239..1834
                     /locus_tag="VLL06_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSS29898.1"
                     /translation="MSIRDRNERRVCDSIQDGMDANKPMDRIEINYRALLAVAVVLNS
                     QREMQSLWEAITAEITKVVPWARATVTLYDHETDGFKFYVIATTMAQVVLQRDAVIPR
                     AGSGMGWAYDHKMLHIRPDLKRVQVFPEDKLYVQEGLGRIINLPLLVGDKCLGILNIG
                     SIESGVPDPGDLEFLTQVAMQIAYAIDHVQAYEQIDRLRNQLAKENVYLIEELKLTKY
                     SGSLVGKSPAFLNVLALAREVAPSPMTVLIMGETGTGKELIAQAIHDWSPRHRKPLVR
                     VNCAAFPAGLVESELFGHERGAFTGADRTREGRFELADGGTLFLDEIGEMPLETQAKL
                     LRVLQDGMVDRLGGKQPVPVDVRVIAATNSDLTAAVKHGTLRADLFYRLNVFPLGLPP
                     LRDRLEDIPLLARHFLGHYSVKFNRPCRDIDQESLERLMRYSWPGNIRELENVIERAL
                     ILSHEPVLRINEHVLGMQGSSFKASPPSDLNELERRHILQTLTWTEWHIEGPDGAAAR
                     LGLPPSTLRSRMKQFGMKRPPAS"
BASE COUNT          456 a          516 c          575 g          460 t
ORIGIN      
        1 aatctttcgc cctgttccat cggtcgggta gcctgcgact aagtctcaag agatatcgat
       61 tgatggttta gggttgcagg tcatgaggcg ggagcggacc tcccagaggt gcaaacatca
      121 atacgcagga cgtgctctat tgctccatcc atttggctgc cttgaggttg agggtacgaa
      181 tgaaagaaag ctcgacgatg ctcctctttt gagtgatcga gttttgcatg agaaagtgat
      241 gagtattcgt gatagaaatg aacggagagt ctgtgattca atccaagacg ggatggacgc
      301 gaacaagcct atggaccgga ttgagataaa ctatcgagcc ctcttggcag tcgctgttgt
      361 gctcaattcg cagcgggaga tgcagagcct ctgggaagcg atcaccgctg aaatcactaa
      421 ggtggttcct tgggcacggg cgactgtcac cctgtatgat catgaaaccg atggatttaa
      481 attttatgtc attgccacga cgatggcgca ggtggtactc caacgcgacg cggtgattcc
      541 ccgtgctgga agcgggatgg ggtgggcata cgatcataaa atgctccaca ttcgaccgga
      601 tctgaagcgc gttcaagtgt ttcctgaaga caaattgtac gtgcaagaag gcctcggacg
      661 cataatcaat cttccgcttc tcgtaggaga caagtgtcta ggcattctca atattggcag
      721 tatcgagtcg ggagttcccg acccagggga tctggagttt ctcacgcaag tggcgatgca
      781 gatcgcctat gcgatcgacc atgtccaagc ttacgaacag atcgatcgat tgcggaacca
      841 actggccaaa gagaacgtct atctaatcga agaactgaag ctcacaaagt acagcggctc
      901 attggtgggg aagagtcctg cctttctgaa tgtgctggcc cttgcgcgcg aggtagcacc
      961 ttctcctatg accgtgctca ttatgggtga aacgggcacg ggcaaagaac tgattgcaca
     1021 ggcgatccat gattggagtc cacggcaccg gaaacctttg gttcgggtca actgcgccgc
     1081 ttttccggct gggctggtcg aaagcgaatt atttggccat gaacgaggtg cgttcaccgg
     1141 ggccgaccga actcgcgaag gccgctttga actggccgat ggcggcaccc tcttcttgga
     1201 tgaaattggg gagatgccat tggagaccca ggccaagctc ctccgtgtct tacaagacgg
     1261 catggtggat cggttgggag ggaaacagcc ggtgccggtt gatgtgcgtg tgatcgccgc
     1321 caccaatagc gatttaactg ccgcagtcaa acacggcacc ttgcgcgccg atctctttta
     1381 tcggctgaat gtgtttcctc tgggcttgcc cccactacgg gatcgcctgg aggatatccc
     1441 gttgttggcg cgacattttc ttggacatta cagtgtgaag ttcaaccggc cgtgccggga
     1501 tattgatcag gaatcgctcg aacggttgat gcgctatagt tggccaggca atatccgcga
     1561 actggaaaat gtcattgagc gcgcactgat tctctcgcat gaaccggtgt tgcggatcaa
     1621 tgagcatgtc ttggggatgc aggggtcctc cttcaaggcc tcgccaccgt ctgatctcaa
     1681 cgaacttgaa cgtcggcaca ttctgcagac cctcacctgg accgagtggc acatcgaagg
     1741 gcctgatggc gcagcggcgc ggttgggact cccgcccagc acattgcgca gccggatgaa
     1801 acagtttggg atgaagcgac cgcctgccag ctgaagaaac ccaccacctt tctgcatcgt
     1861 ctctttaagc cctctcagga caaatcgccc gtgatccgtc gtgagcggag cccgcaacga
     1921 tcgcccttca catcatccgg gacgatgttc cctgtgattt ctaactggtc ctcaagcgca
     1981 tcacgtgcct cgttgagaag tgagcaa
//
LOCUS       DATIPF010000107         1894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1079852, whole genome shotgun sequence.
ACCESSION   DATIPF010000107 DATIPF010000000
VERSION     DATIPF010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079852"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..617)
                     /gene="mobB"
                     /locus_tag="VLL06_02665"
     CDS             complement(93..617)
                     /gene="mobB"
                     /locus_tag="VLL06_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HSS29899.1"
                     /translation="MAVPIVSFVGRSNSGKTTLIERVIPELVRAGYKVATVKRTGHGF
                     DLDTEGKDSWRHKQAGASAVLVLSKGSMAMFADADDQMNVEDVRDRFLDHTYDLIIAE
                     GWKHEGYQKIVVVRDQIGEVPLSTEGLLAVVSDKPVDLSAPLFGRDDFVGVAALIMKQ
                     FPRSQSCAEHGLET"
     gene            complement(701..>1894)
                     /gene="glp"
                     /locus_tag="VLL06_02670"
     CDS             complement(701..>1894)
                     /gene="glp"
                     /locus_tag="VLL06_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HSS29900.1"
                     /translation="DALGRVLGEDIVAERDNPPWNNSAMDGFAVRWVDIRQEHAIQKP
                     VTLAVIEDVAAGKMPSKSVGAGQAIRIMTGAPLPKGADTVLKVEDTEHTSGSVRVFKP
                     EQQGANIRPQGEDVKKGECIIGKGTRIRPGEAGMLAILAKSFIFAYQRPRVAILSTGD
                     ELADLDERFSEDKIINSNSYGIAAAVQEAGGIPLLLGIARDTPAALKEKISHGLTADI
                     LVLSGGVSMGDYDFTKAVFQELGAEMHFWKLAIRPGQPLAFGKIQGKLAFGLPGNPVS
                     SMMTFEQLVRPALLKMSGHPNYGRPVVQAIFQEAFSKRTDRRHFLRGVLTREDGVFMV
                     RTTGAQGSGILTSMVKANCLIDIPVEVERVSPGDLVSVQLLTGEVWASSSDPGQTSSH
                     HLSCC"
BASE COUNT          411 a          589 c          477 g          417 t
ORIGIN      
        1 cgcagcgagc gaagaggcga ggcgtacgct ttcggtacgt tgagcctctg agtgacgcga
       61 gaacgccgct ggcgggcttt ttcagcatcc tgttaggttt ccagtccatg ctccgcacaa
      121 gactgcgatc ttggaaactg cttcatgatc aaggcggcca caccgacaaa atcatcccgg
      181 ccgaacaagg gtgcggatag atcgacgggc ttgtccgata ccactgcgag cagcccttca
      241 gtggagagag gaacttcgcc gatctgatca cggacaacga cgattttctg atatccctcg
      301 tgtttccatc cttcggcaat gatcaggtca taggtatgat cgagaaatcg atcacgcaca
      361 tcttcgacgt tcatctgatc atctgcatcg gcaaacatcg ccatacttcc cttggaaagt
      421 accagcacgg cactggcgcc ggcttgcttg tggcgccagc tgtccttccc ttccgtatcg
      481 agatcaaatc cgtgtccggt ccgcttcacc gtcgccacct tgtaaccggc ccggaccaac
      541 tcagggatca cacgctcgat cagggtggtc ttcccactgt tcgaccgacc gacaaacgag
      601 acaataggta cagccatgaa aagtgagccc tttgttatgt gtcacgaatc aatcgtgagt
      661 gaaccgccgc actgccttgc tcagcgcttc ccccgctgcc tcagcagcag gagaggtgat
      721 ggctgcttgt ctggcctgga tcactggagc tggcccagac ttctccggtt aacagttgaa
      781 ccgataccag atcgcccggg ctcactcgtt caacttcgac ggggatatcg atcaaacaat
      841 tggccttcac catagaggtc aatattccag acccctgcgc accagtcgtt cgaaccatga
      901 agacaccatc ttctcgagtg agcacgccgc gtagaaagtg gcgtcggtcc gtccgtttgg
      961 aaaatgcctc ttggaagatc gcctgcacaa ccggacgacc gtaattcgga tgtccgctca
     1021 ttttgagcag ggccggccgt acaagctgtt cgaatgtcat catggaggac acgggattcc
     1081 ctggcaagcc aaaggccagc ttaccctgaa tcttgccgaa agcaagcggc tgccccggcc
     1141 gaatcgccaa cttccagaag tgcatctctg cgcccagttc ctggaagacc gccttcgtaa
     1201 aatcgtaatc gcccatcgac acgccaccgg acagcaccag aatgtctgcc gtgagtccat
     1261 gcgaaatctt ttcttttagt gccgcaggtg tatcccttgc aatccccagc aacagaggaa
     1321 tgccacccgc ctcctgaacg gctgccgcaa ttccataact attggagttg atgattttgt
     1381 cttcgctgaa ccgctcgtcc aaatccgcca attcatcacc ggtcgaaagg atcgccaccc
     1441 gaggccgttg ataggcaaaa atgaatgact tggcgagaat cgccaacatc cccgcttcac
     1501 ccgggcgaat cctggtccct ttcccgatga tacattcgcc cttcttgacg tcttcgcctt
     1561 gagggcgaat gttggcgccc tgctgttccg gcttgaagac acgaacggaa cccgacgtat
     1621 gctcggtgtc ttcgaccttc aagaccgtat ctgcaccctt cgggagcggg gccccggtca
     1681 tgatgcgaat cgcttgcccc gcgccgacgg attttgaagg cattttgcct gcagccacat
     1741 cttcaattac tgcaagcgtc actggtttct gaatcgcgtg ttcctgcctg atatcgaccc
     1801 accggaccgc aaacccgtcc atcgcactat tgttccaggg cggattatcc cgctccgcca
     1861 cgatgtcctc gccgaggacc cgtccaaggg cgtc
//
LOCUS       DATIPF010000108         4000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_19234, whole genome shotgun sequence.
ACCESSION   DATIPF010000108 DATIPF010000000
VERSION     DATIPF010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19234"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="VLL06_02675"
     CDS             complement(<1..1149)
                     /locus_tag="VLL06_02675"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HSS29901.1"
                     /translation="MGRVAGKKLFSVMALCAMIGLLLLPIAVSLPSLAIGADAPDAVK
                     KDGEAKVEKGRDVYYKTEGIVVGAPAPKTTDGPKDYPRYNFESRVLLWFANQQHLYYG
                     SFVLAVPIFCMVIEFMGVVSKDKAMAKRYDQLAHDFIKISLTAYSLTAILGGVLIFTF
                     LTLYPAFFGYLSSIFRPVMHIYALMFIAESGTLYIYYYGWDKMTTGFLKWIHLSMSVV
                     LNVIGTLLMFLANSWIGFMMSPAGVDEQGRFLGNIWHVIHTALWNPLNVHRILGNMAF
                     GGGVVAAYAAYKFLASKTDEDRAHYDWMGYIAMALGVAFLIPLPFAGYWLMREVYAYR
                     QQMGITLMGGLLAWLFIIQATMIGILFLSTNYYLWQALGRMRGAEKYQR"
     gene            complement(1801..2670)
                     /locus_tag="VLL06_02680"
     CDS             complement(1801..2670)
                     /locus_tag="VLL06_02680"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSS29902.1"
                     /translation="MLETKFKVLFLLAILGFAAIPIMGILRGSPLTPFEKAPAESTQS
                     EALDPSQASREEPVREEMIVIPAGPFIRGTDHGGLDEQPQRTLVLDAFALDRYEVTNF
                     QYQQFVKVTGHRKSGPPARYAKNMSKMQGVNQPVVYVSWEDAETYCHWKGKRLPTEAE
                     WEKAMRGSDGRLWPWGNVEQPNGANWARVQDGHDVSASVGAFQTDKSPYGVMDGAGNV
                     MEWVADWYAERYFEEAPERNPPSPDHGVFRVLRGGGYATTGADVRITSRSKMVPDFRD
                     EAIGFRCASSELP"
     gene            complement(2673..3599)
                     /locus_tag="VLL06_02685"
     CDS             complement(2673..3599)
                     /locus_tag="VLL06_02685"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSS29903.1"
                     /translation="MGNRGVLIGSIVFVFGSFILMVSLLLYESYKANKMKALASSIKT
                     EARPATSSAPAVDYSMYKTKISDEGREMVQVPEGPFMMGSKDGDPDEAPERQTYLKAF
                     YIDTKEVSQEEYARFAKMTKRALPKIEVFEDDQSKLLRPEFAAMSMTWDEAVAYCKWA
                     GKRLPTDAEWEKAGRGEGKRKYAWGDTFMSGRANANVDGSEDGFRYLAPPGSFETGRS
                     PYGIYDMTGNVAEWVADSYDEHYYQKAPYRDPKGPEPGELKVVRGGSWRETQHNARLS
                     KRFAAKLWRTDITIGFRCASDVEVGDSPTASK"
     gene            complement(3649..>4000)
                     /locus_tag="VLL06_02690"
     CDS             complement(3649..>4000)
                     /locus_tag="VLL06_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29904.1"
                     /translation="ESSMGYAAPSRVIDILFGVAVMGTVGVLIGLIMGGNIMPVASGA
                     GLVLGGFIGFMGGRRFLVSILVGTVLGGALAWWIAGPERISAGAGAGAAMGGFLGVQI
                     SMLLDMRAAKKKRV"
BASE COUNT          963 a         1223 c          915 g          899 t
ORIGIN      
        1 gcgttgatat ttttcagcgc cgcgcatccg acccagtgcc tgccacaaat aataattcgt
       61 gctcaggaag aggatgccga tcatggtggc ttgaataatg aagagccagg ccaggagacc
      121 gcccatgagc gtaatgccca tctgctggcg ataggcatag acttcgcgca tgagccaata
      181 cccggcgaat ggcaagggaa tcaggaacgc cacgccgagc gccatggcga tataacccat
      241 ccagtcataa tgcgcgcgat cctcatctgt cttggaggct aagaatttgt acgccgcata
      301 cgccgccacg acaccaccac cgaacgccat attacccaaa atacggtgca cgttgagcgg
      361 attccagagg gcggtatgaa tgacatgcca gatgttcccc aggaaccggc cttgctcatc
      421 gacgccagcc ggcgacatca tgaagccaat ccaggaattc gccaggaaca taagcaacgt
      481 accgatcacg ttcaacacca cggacatact caagtgaatc cacttgagga acccggtcgt
      541 catcttatcc cagccataat aatagatgta gagagttccg ctctccgcta tgaacatcag
      601 tgcatagata tgcatgactg ggcggaagat actggacaag tagccaaaaa acgctgggta
      661 gagcgtcagg aaggtgaaaa tcaacacacc accaagaatt gcggtcagtg aataggccgt
      721 cagactaatc ttgatgaagt catgggcgag ttggtcatac cgttttgcca tcgccttatc
      781 cttcgacacc acccccatga actcaatcac catgcagaaa attggaactg caagtacgaa
      841 actcccataa tagaggtgtt gctggttcgc aaaccagagc aacacgcggc tctcgaagtt
      901 gtaccgcgga taatccttag gaccatccgt ggtcttcgga gcaggggccc cgacgacgat
      961 accctctgtc ttataataga catctcgccc cttctcgacc ttcgcctctc catccttctt
     1021 cacagcatcc ggtgcatctg cgccaatagc cagtgatgga agcgatacgg caatggggag
     1081 caggagaagg cctatcatcg cgcaaagcgc catgaccgag aacagcttct tcccggctac
     1141 acgtcccatg agatacctcc ttgatgcatt cttaaaaaaa ctcatgccat ttcttttcaa
     1201 gctcaaatcg aaatagaacg gcaatccgcg ttctatttcc ggaataaata ccagaagtcg
     1261 agtccctgcg aatccattaa aaagtgatca acagcggtaa agtacgcgat gcagatgact
     1321 aaagagatca gaccgtaaag aattgcactt cccatcgcat tcctccctca gactttgcga
     1381 acgtcccaaa aattaaaacc tggccgtacc ccgtttagca ggggcattga attccagcga
     1441 accaatagaa aaaccacagg cccgccgcgc aggttatata aaatatcatc ttgccgattg
     1501 cgatttttat ttcactgtcc ggtgttgtgg atacttctgt tgccgtggcc atctcgtctc
     1561 cttattcctg atgcaaacca tcaatcccga atcagcttag cgaatttctt gacactcaga
     1621 aacccgtatg ttattcagaa ttccttgcca cacaggcaaa ttcaggggaa aaccgatgaa
     1681 aaagaaatca aaagtgttcg acattatagt tttggccgga atggttgtca atatacttgt
     1741 ggccgtgttt ctcgttctct actactttga tatcctgtaa gttctgaatg gtttcccccc
     1801 tcagggcagc tccgaactcg cacagcgaaa tccgattgct tcatcgcgaa aatccggaac
     1861 cattttgctt ctgctggtga tccgaacatc ggctcccgtg gtcgcatatc ctcccccccg
     1921 aagtacccga aataccccat gatccgggct cggcgggttc cgttcaggag cctcctcgaa
     1981 gtagcgttcc gcataccagt cggccaccca ttccatgaca tttccagccc catccatgac
     2041 accgtacggg ctcttgtcgg tctgaaacgc accgacggat gcggaaacgt catgcccatc
     2101 ctgcaccctg gcccaattcg ctccgttggg ctgttccaca ttgccccagg gccagagacg
     2161 gccatccgat ccccgcatgg ccttctccca ctcggcctcg gttggaagcc gcttcccttt
     2221 ccagtgacag taggtctccg catcctccca tgatacatag accacaggct ggttgacgcc
     2281 ctgcatcttg ctcatgttct tggcataacg ggcaggagga cccgatttgc gatgtccggt
     2341 caccttgaca aattgctggt attgaaagtt cgtcacttcg taccggtcga gtgcaaacgc
     2401 atccagcacc agcgtgcgct gtggctgctc atccaaccct ccgtgatcgg tgccccggat
     2461 gaagggtcct gccggaatca caatcatttc ctcccgaacc ggttcttcac gagaggcctg
     2521 cgaaggatca agtgcctctg actgggtgga ttcagccgga gccttttcga atggtgtgag
     2581 cggggaacca cggaggattc ccatgatagg aatcgcagca aacccaagaa tggcaagaag
     2641 aaagaggacc ttgaacttcg tttcgagcat gtctattttg acgccgtggg actgtcgccg
     2701 acctccacat cactcgcaca gcgaaacccg attgtgatat ccgtccgcca caacttcgca
     2761 gcaaatcgtt tggatagccg agcgttgtgc tgggtctctc gccaggaccc tccccgcacc
     2821 actttcaatt cacctggttc cggccctttc ggatcgcggt atggggcttt ctgatagtag
     2881 tgttcatcgt acgaatcggc gacccattcc gcgacgttcc cggtcatgtc gtagatccca
     2941 taggggctac gcccggtctc aaatgagccg ggaggcgcga gataccgaaa cccgtcctca
     3001 cttccgtcca cattcgcatt ggcacggccg ctcataaagg tgtcgcccca cgcgtacttc
     3061 cgctttccct cacctcgacc ggctttttcc cactccgcat ctgtgggaag tcgctttcca
     3121 gcccacttac aataggccac cgcctcgtcc catgtcatgc tcattgccgc aaactcaggc
     3181 ctcagcagct tggattgatc gtcctcaaac acttcgatct tgggtagtgc ccgcttggtc
     3241 attttggcaa accgggcata ttcttcttgt gacacctctt tcgtgtcgat atagaacgcc
     3301 ttgagatacg tttgacgctc cggcgcctca tccggatcgc catctttgct gcccatcata
     3361 aatgggccct cgggaacctg taccatctct cggccttcat cgcttatctt cgtcttgtac
     3421 atggaataat ccaccgcagg tgcactgctt gtcgcagggc gagcttcggt cttaattgac
     3481 gacgccagcg ccttcatctt attcgctttg taggactcat agagcagcaa actcaccatc
     3541 aggatgaagg atccaaacac aaagacgatc gaaccgatga gcactcccct atttcccatc
     3601 ctgtctcatg ctccttcgat tgcgcgtgac atggagtgat cagtcctctc agacgcgttt
     3661 tttctttgcc gcacgcatat ccagcaacat cgagatctgt acaccaagaa atccacccat
     3721 agccgccccg gcccccgcgc ccgcggatat tctctccggg cccgcaatcc accaagccag
     3781 tgccccgcct aacactgtac ctaccaaaat actcaccaga aaacggcgac cacccataaa
     3841 cccgatgaag ccgccgagga ccagccccgc cccactcgca accggcatga tgttaccgcc
     3901 cataatgagc ccgatcagca ccccgaccgt tcccatcacg gccacaccga acaggatatc
     3961 aatcaccctt gaaggtgccg cgtatcccat actggactca
//
LOCUS       DATIPF010000109         4474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_549747, whole genome shotgun sequence.
ACCESSION   DATIPF010000109 DATIPF010000000
VERSION     DATIPF010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549747"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /gene="trpD"
                     /locus_tag="VLL06_02695"
     CDS             complement(<1..786)
                     /gene="trpD"
                     /locus_tag="VLL06_02695"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HSS29905.1"
                     /translation="MIKDAITKLADRSSLTEQEAESVMREMMDGAATPVQIAAYLMGL
                     RLKGETVDEIVGSVRAMRAKALRIVVADPLVVDTCGTGGDGAHTFNISTTAAFIVAGA
                     GLTVAKHGNRSVSSRSGSADVLSALGVKIDLPTERVADCINEVGLGFLFAPLYHGAMK
                     HCSGPRQEMGIRTMLNLLGPLTNPAGATIQVLGVYESRLTHLLGNVLMHLGSQHCFVV
                     HGMDGLDEITLTTKTQISEAKGGILSNYLLDPAEFGLALVPAKQ"
     gene            complement(812..1375)
                     /gene="pabA"
                     /locus_tag="VLL06_02700"
     CDS             complement(812..1375)
                     /gene="pabA"
                     /locus_tag="VLL06_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HSS29906.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGEDVRVFRNNKITIDQIESLKP
                     SRIVISPGPCTPTEAGVSVETIRHFAGRLPLLGVCLGHQSLAAAFGGEVVRAERLMHG
                     KTSKIRHDGKTIFRQLPSPFDATRYHSLIVKRETLPSCFEVSAETAEGEIMGMRHRSF
                     GIEGVQFHPESILTMVGKDLLRNFLKL"
     gene            complement(1449..2093)
                     /locus_tag="VLL06_02705"
     CDS             complement(1449..2093)
                     /locus_tag="VLL06_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HSS29907.1"
                     /translation="MSRGVELVGSSPETLVRCENNVISVRPIAGTRRRGGTPEEDQRM
                     ERRLLADEKERAEHVMLVDLGRNDVGRVAKPGSVTVDSLMHVERYSHVMHIVSNVTGQ
                     LDSSKTSYDVLRACFPAGTVSGAPKVRAMQIIDELEPTRRGPYAGAVGYFSFSGNMDM
                     CINIRTVVVKKHQAFIQAGAGIVADSNPEHEYEETCNKAQAMMKAIELAEQGLE"
     gene            complement(2038..2946)
                     /locus_tag="VLL06_02710"
     CDS             complement(2038..2946)
                     /locus_tag="VLL06_02710"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HSS29908.1"
                     /translation="MAPPQYSLSLDQLRQYAGEGNLVPLYREILADYETPVSAFAKID
                     HGPSAYLLESVEGGEKWARYSFLGSGSPVVVYEDGGDLLVLRGTRKKRIPSRGNPLER
                     LREMMESYRPVTVPDLPPFVGGAVGYLGYDMVRTFEDLAARRKDPLNFPDFAFLLTDT
                     MLIFDNVAQKIKVVATAHVTARGERGIKAAYRSATERIERTITRLKRPLRRTRLHRRR
                     RPVTFTANMSKADFEKMVVRTKEYIGSGDIDQAVLSQRWEANVQAPPLQLYRALRVVN
                     PSPYMYYLRVAGGRAGWFFSGDARPL"
     gene            complement(2956..3588)
                     /locus_tag="VLL06_02715"
     CDS             complement(2956..3588)
                     /locus_tag="VLL06_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HSS29909.1"
                     /translation="MKGENHVNRLMSRSSYRVAAIICGGLLMGGCVMAEKYDAEKARS
                     LNFQRLLAQEEKRTGELDSEAKRSKNELMEYEARNRELMAQLQSSREQLARVQEEAEA
                     IKESALLERKAKTDMKRALAPVRKSPAVSTPDSGDLSASALGLNPETGSTDSMRGSMR
                     SPAVHTVKPGETLFRISRQYGVTVDKVRKWNKLPDDIIEVGQKLIVGQEY"
     gene            3806..>4474
                     /locus_tag="VLL06_02720"
     CDS             3806..>4474
                     /locus_tag="VLL06_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29910.1"
                     /translation="MAHADILASLIALRDEIRRHDYLYYVKDRPEISDSQYDHLFREL
                     LELEQAHPELVTPDSPSQRVGAPPLEELIKVPHEQPMLSLDSIVDQSEVQAFDQRMKR
                     ELETLSVEYSAEPKFDGLSVELIYDRGMLTRGATRGDGTTGEDVTINIRTIRSLPLQL
                     HAQSSFPDHLVVRGEVYMRLDDFQALNRRITERGDDAFANPRNAAAGSLRQLDSTITA
                     TRPLV"
BASE COUNT         1005 a         1329 c         1158 g          982 t
ORIGIN      
        1 ctgtttggcc ggaaccaacg caagtccgaa ctctgcagga tcgaggagat agttggaaag
       61 aatcccccct ttggcttctg aaatctgcgt tttagtagtc agagtgatct catcgagtcc
      121 gtccatccca tggacgacaa aacaatgttg cgatcccaga tgcatgagca cattccccag
      181 caagtgggtc agtcgacttt catagacacc aagtacctgg atcgtcgctc ctgccggatt
      241 cgtgagcggg cccaaaagat tcaacatggt ccggataccc atttcctgtc ttgggccgga
      301 acaatgtttc atggccccat gatagagcgg ggcgaagaga aagccgagtc caacctcatt
      361 gatacaatca gccacccgtt cggtcggcag atcaatcttg acccccagtg ccgagagcac
      421 atctgcgctc cccgacctag atgatacaga acggttgccg tgcttggcca cagtcaaacc
      481 tgcgcctgct acgataaacg ctgcagtcgt ggaaatatta aacgtatggg caccgtcccc
      541 acccgtgcca caggtatcca cgaccaatgg gtctgcgaca acaatgcgga gggccttggc
      601 acgcatggcc ctcacggaac cgacaatttc gtcgacggtc tctcccttca gacggagccc
      661 catcaagtag gcggctattt gaactggagt ggctgcgccg tccatcatct cacgcatcac
      721 cgattcggct tcttgttcgg tgagggaaga gcggtcggca agtttggtaa tggcgtcttt
      781 gatcattgga taggagacca ggcgggtcag gctacagttt gaggaagtta cgaaggaggt
      841 ccttccctac catggtgagg atcgattccg gatgaaactg cacgccttcg atgccgaacg
      901 agcgatggcg cattcccatg atttcaccct cggccgtttc agccgagacc tcaaaacagg
      961 aaggcagcgt ctctcgcttc acaatcagag aatggtagcg agtggcgtca aacgggctcg
     1021 gcagctgacg aaagatggtc ttcccgtcat gcctgatctt cgacgtttta ccatgcatca
     1081 accgctcggc tctcacgact tccccgccaa acgccgcggc caaagattga tgaccgaggc
     1141 agacacctaa gagcggaagt cgcccagcaa aatgccggat cgtctcgacc gacacgcccg
     1201 cttccgtcgg tgtgcagggt cccggagaaa tgacgatgcg gctgggtttc aacgattcga
     1261 tctgatcgat cgtaatttta ttgttgcgga atacccgaac gtcttcccca agctccccaa
     1321 aatactgcac gaggttatag gtaaaggaat cgtagttgtc gatcatcagt aacatacgtc
     1381 aaccatccgg gatggctcgt caggaggaaa caacggcgag acgctgatgg cctcaccgcc
     1441 ctcccagttc attccaaccc ttgttccgcc agctcaatcg ccttcatcat ggcctgagcc
     1501 ttattgcagg tctcttcata ttcatgttcc ggattcgaat ccgcgacaat gccggcccca
     1561 gcttgaataa atgcctggtg ctttttcacg acgaccgtcc ggatattaat gcacatgtcc
     1621 atgttgccgg agaagctaaa atagcctacc gcgcccgcat agggaccgcg tctcgtgggt
     1681 tcgagttcgt cgatgatctg catcgctcga accttgggcg caccggagac tgtgccggca
     1741 gggaagcagg ctcgcaagac gtcgtacgag gtcttcgacg aatcaagttg ccctgtcaca
     1801 ttggacacaa tatgcatcac gtgcgaatag cgctcgacat gcatcagtga atccaccgtg
     1861 accgaacctg gtttcgccac acggccgaca tcattccgcc caagatcgac gagcattacg
     1921 tgctcagccc gttccttttc atccgccaag agccgccgtt ccatccgttg atcttcttca
     1981 ggcgtgccgc cacggcggcg cgtacccgca ataggacgga cggaaatgac attattttca
     2041 caacggacga gcgtctccgg agaagaacca accagctcga ccccccgcga cacgcaggta
     2101 atacatatag ggagagggat tcaccacgcg caatgcgcga tagagctgca aaggcggcgc
     2161 ctgcacgttg gcctcccatc gctgggataa gaccgcttgg tcgatatcac cggacccaat
     2221 gtattccttc gtccgcacga ccatcttctc aaagtctgcc ttgctcatgt tcgccgtaaa
     2281 cgtcactggt cggcggcggc gatgcaaccg cgttcgtcgg agcggacgct tcagcctcgt
     2341 gatcgtacgt tcaatccgtt ccgttgccga tcggtaggcg gccttgatac cacgttcccc
     2401 cctcgccgtg acatgtgcgg tggcaacaac tttgatcttc tgggccacat tgtcgaagat
     2461 cagcatcgta tcggtgagca ggaacgcaaa gtccgggaaa ttgagcgggt ccttcctccg
     2521 ggcagccaaa tcctcgaacg tccgcaccat gtcgtagcct agatatccca cagcgccgcc
     2581 gacgaacggg ggaaggtccg gcacggtcac ggggcgataa ctttccatca tctccctgag
     2641 acgctcgagc ggattcccgc ggctgggaat gcgcttcttg cgtgtgcctc gaaggacaag
     2701 caggtcgccc ccatcctcat agacgacgac gggcgaacca ctcccaagaa aggaatagcg
     2761 cgcccatttt tcaccaccct caacggactc taagaggtag gctgaaggac catgatcgat
     2821 tttggcaaag gcagaaacgg gggtctcata gtctgcgaga atctctcgat agagcggaac
     2881 gaggttgccc tctccagcat attggcgaag ttgatcgaga ctgagtgaat attgcggcgg
     2941 agccacgtgg ttgtcctagt attcttgtcc cacgatgagc ttctgtccca cttcgataat
     3001 atcgtccggc agcttattcc atttcctgac cttatcgacc gtgacaccat attgccgact
     3061 gatccgaaag agcgtttcgc caggtttcac agtatgaacg gcgggcgatc gcatgcttcc
     3121 acgcatcgaa tctgttgacc cagtctccgg attaagcccg agagccgacg cactcaggtc
     3181 cccggaatct ggcgtggata cagccgggga ctttcgcacc ggtgcgagcg cgcgcttcat
     3241 gtctgttttg gctttgcgtt ccaacagcgc agactccttt atcgcctcgg cttcctcctg
     3301 cacgcgggcc agttgctccc gagaggattg caactgtgcc atgagttcac gattcctggc
     3361 ttcatactcc atcaattcat ttttgcttcg cttcgcttca ctatcgagct caccggttcg
     3421 tttctcttcc tgagccagaa gacgttggaa atttaggctg cgtgcctttt ccgcatcata
     3481 tttctcggcc atcacacatc cgcccatcaa cagtcctccg cagataatgg cggcgacccg
     3541 ataacttgac ctggacatca acctattcac gtgattctcc cctttcatgg atatctcctc
     3601 tgatgatcgt gagagagctt ccctctcgca gtaagaggtg aaaactcgct gccggtcagt
     3661 ggaaatcgac tcagctctgt gcgttacaat aatgtctatc gcggcgaaag tcaaagcgaa
     3721 taggcacctt ggtcgtttga cccagtccca atccctatga tacggtcgaa gccgtcgcta
     3781 cctctgttcg tcaccatcca gtcctatggc ccacgccgac atccttgcca gtctgattgc
     3841 cctcagggat gaaatcaggc ggcacgacta tctgtactac gtcaaggatc gcccggaaat
     3901 ttctgattcc cagtacgatc acctgttccg agagctgctc gaactggaac aggcccaccc
     3961 cgaactggtg acgccggatt ctccgtcaca acgtgtcggc gcaccgccgc tcgaagagtt
     4021 aatcaaagtc ccccacgagc agccgatgct cagcctggac tccattgtcg atcagagcga
     4081 ggttcaggca ttcgaccaac ggatgaaacg tgaactggaa accctgtccg ttgaatacag
     4141 cgcggaacca aaattcgatg ggctatcggt cgaactgata tatgaccgtg ggatgttgac
     4201 ccgtggagcg accagaggcg acggaacgac tggagaggac gtgaccatca atattcgcac
     4261 gattcgttcc ctaccgctcc agctgcacgc acaatccagc ttccctgatc atctggtcgt
     4321 gcgaggtgaa gtgtatatgc gactcgacga tttccaagcg ctgaatcggc gcatcacgga
     4381 acggggcgac gacgcgtttg ccaatccacg taatgcagcc gcgggttcgc ttcgacaact
     4441 ggattctacg attaccgcga cccgaccatt ggtt
//
LOCUS       DATIPF010000110         3485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_550161, whole genome shotgun sequence.
ACCESSION   DATIPF010000110 DATIPF010000000
VERSION     DATIPF010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550161"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VLL06_02725"
     CDS             complement(<1..513)
                     /locus_tag="VLL06_02725"
                     /inference="COORDINATES: protein motif:HMM:NF023081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HSS29911.1"
                     /translation="MSNNLLTISFITNEGLLVLENNLVFADKVDRQYSDEFAIKGAKI
                     GATCNVRRPPRYLGTFGPALNVEDTNETYVPVSLNYQFHVDVQFTTADLLLSMDLFRT
                     RVLKPMMATVANRIDNDGLYFAYQNTAQAVGTFGVTPSSYLTFANADAQLSNEAMPEE
                     NRNMLLSPIMM"
     gene            complement(547..1524)
                     /locus_tag="VLL06_02730"
     CDS             complement(547..1524)
                     /locus_tag="VLL06_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29912.1"
                     /translation="MVQVVTNENMQEFIAKREVPAFVAPKVEVKPVEKTAEAVATEGK
                     QEQARAEDGKFVKAEAKTEEKSSEAAKTAVDDEDADLPERVKRIIGKKHRGMKEAEEF
                     ARERDRQAAVAEAQVEELKRQLQEFAKSGPAQDGKSQAKADGAPNPEDFKTVAEYADA
                     LVEFKLTKRLAEREQEQTQKQQQQAADQVKSAFIERLEKARETITDYDDVVGAVDTQI
                     PPHMAQYVMESDLGPLLGYHLAKHPEEIQRLVKLSPIRAIAELGKLEASLEKKPEVRP
                     AVIPAVSKAPSPIEPLDGGSATVNKDPAKMTLQELREFRRTEDAKRARR"
     gene            complement(1524..>3485)
                     /locus_tag="VLL06_02735"
     CDS             complement(1524..>3485)
                     /locus_tag="VLL06_02735"
                     /inference="COORDINATES: protein motif:HMM:NF027825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HSS29913.1"
                     /translation="KIEGRPALTINHTNVQVSRLKNTLKQQRPRIKCHPVGDGADVDT
                     AEVVNGLIRHIETLSDASVAYDVGVSSAVDIGWGYWRILSEYISPTSFDQELKIAPIR
                     NPFTVYMDPTAKHPAGLDQGWCIISETMKRAEYKRKYPRAENSDWRYVDAPGDMTLDW
                     ESKEEIRLAEYYRIHEVRDTLCQLSDGATKYKSELYSPEVMAAAGVKVVNERPTTRCV
                     VQWFRLNGKAIVDKRDLPGYHIPVVRCEGNVLDMNGRVRRKGMVYDLMDPARIYNYTE
                     TQKTERYALTPKAPWVGFEGQFDGHPEWVDANQKSYSTLVHKPVQLPDGSIAPPPQRT
                     PPAAVEAGMAEWSASAERNLMSVAGMPQENPEISAKVVGGNKYLQRRQGMQDLVHFQY
                     YDNQTLAIASTGVLLLELIPFYYDTPRMQRIIREDGVPEMVKLKQTYEEDGVQKVKND
                     PTVGRYDVVMDTGPGYATKREEAAESMLELLNTKLGEKVADTSGDIVLRNMDFPGSSE
                     VADRVATTIPGALDKIVENLPKQAQTIVGSLQAQLKQAGDMIQKLQMEIKYKSGVETM
                     KDQGQTHRTEIQATTQLHKTAMEVKAEDANSERDYVGWMRDVKERGDAQRDVAEIHAA
                     AQLLNTHVEAAHEAKAADKLIKAGTERRE"
BASE COUNT          659 a         1065 c          888 g          873 t
ORIGIN      
        1 catcatgatc ggtgagagca gcatgttgcg gttctcttcc ggcatggcct cattggacag
       61 ttgcgcatcg gcgttggcga aggtcagata tgaactcggc gtaacgccga atgtacccac
      121 cgcctgagcc gtattctggt atgcgaagta caggccgtca ttgtcgatcc ggttcgcaac
      181 cgtagccatc atgggtttca gtacgcgagt acggaacagg tccattgaca gtagcaagtc
      241 agcagttgtg aactgcacgt ccacatggaa ctgatagttc agggaaaccg ggacgtatgt
      301 ttcattcgtg tcctcgacat tcagcgccgg gccgaaggtg ccgagataac ggggcggacg
      361 ccgcacgtta caggtggccc cgatcttcgc gcccttgatg gcaaactcgt cagaatactg
      421 acggtccacc ttgtcggcga agaccaggtt attttcgagg actagcaggc cctcgttcgt
      481 gataaacgaa atcgtgagca aattgttcga cacagagatg actccagaaa gaggtgacca
      541 ttgccatcac ctccgagctc gcttcgcgtc ttccgtcctg cggaattcgc gcagctcctg
      601 aagggtcatc tttgcagggt ctttgttgac cgtcgcagat ccaccatcca gcggctcgat
      661 tggcgaaggc gccttggaaa ccgctggaat caccgcaggc ctaacctcgg gcttcttttc
      721 cagactggct tccaatttcc ccaattcggc aatagcccga attggcgaca gtttcaccag
      781 tcgctgaatc tcttccggat gcttggccag gtggtaaccc aaaagcggac ccaggtcgct
      841 ctccatgacg tattgcgcca tgtgaggagg aatctgagtg tcaacagcac ccaccacatc
      901 gtcatagtct gtgattgttt ctcgcgcctt ctccagacgc tcgataaagg cagacttgac
      961 ctgatcggca gcttgctgct gctgcttctg cgtctgctcc tgttcgcgtt ccgctaaccg
     1021 cttggttaac ttgaactcga ccaatgcatc ggcgtattca gcaactgttt tgaagtcttc
     1081 agggttggga gcgccgtcag ctttcgcctg tgactttccg tcctgagcag gtcccgactt
     1141 cgcgaactct tggagttgcc tcttgagttc ctctacctgc gcttcagcaa ctgccgcttg
     1201 gcggtcgcgc tctcgcgcga actcctcggc ctctttcatg cctcgatgct tcttgccgat
     1261 aatgcgcttg acgcgctccg gcaaatccgc atcctcatca tctactgcgg tcttggccgc
     1321 ctcagaggac ttctcttctg tcttggcctc agccttgaca aactttccat cctcagccct
     1381 agcttgctct tgtttgccct cggttgcaac tgcctccgca gtcttttcaa ccggcttcac
     1441 ctctaccttg ggagcaacga atgccggcac ctcgcgcttg gcgatgaatt cctgcatgtt
     1501 ctcgtttgtg acgacctgaa ccattactcc ctccgttccg taccggcttt gatcaacttg
     1561 tctgctgcct tggcttcgtg agcagcctcc acatgggtat tgagcaattg ggccgcagca
     1621 tgaatctctg ccacgtcgcg ctgcgcatcc ccgcgctcct tcacatcgcg catccagccc
     1681 acataatcgc gctcactatt ggcgtcctcg gccttaactt ccatcgcagt cttgtgcagc
     1741 tgcgtggtgg cctgaatctc ggtgcgatgg gtctgcccct gatccttcat cgtctcgacg
     1801 ccagacttgt acttgatctc catctgcagc ttctggatca tatctccggc ctgtttgagc
     1861 tgggcctgca gtgagccgac aatggtttgt gcctgctttg gcaggttctc aacgattttg
     1921 tccagagctc ccggaatcgt ggttgcgact cgatccgcca cctcgctgga gcctgggaag
     1981 tccatgtttc tgaggacgat atcccctgac gtgtccgcca ctttctcacc cagcttcgtg
     2041 ttgagcagtt cgagcatgga ttcggcggcc tcttcgcgct tggtcgcgta cccaggcccg
     2101 gtatccatca ccacgtcata gcggcctaca gtggggtcat ttttgacctt ctgtacgcca
     2161 tcctcttcat aggtctgttt gagcttgacc atctccggca cgccatcctc gcggatgatg
     2221 cgctgcatgc gcggggtgtc gtaatagaac gggatcagct ccaacagtag tacgccagtg
     2281 gatgcaatcg ccagggtctg attgtcgtag tactgaaagt gaaccaagtc ctgcatgccc
     2341 tgccggcgtt gcaagtactt gtttccaccc acaaccttgg ctgatatctc gggattctcc
     2401 tgcggcattc ccgccacgga catgagattg cgttccgcac tggctgacca ttcggccatt
     2461 cccgcttcta ccgcagccgg aggggttctc tgtggagggg gagcaatcga cccatctggc
     2521 aactgcaccg gcttgtgaac cagcgtgctg tagctcttct ggttcgcgtc cacccattca
     2581 gggtgcccgt caaactgccc ctcaaagccc acccatggcg ctttaggagt cagcgcatag
     2641 cgctcagtct tctgtgtctc ggtatagtta tagatgcgcg cagggtccat caggtcataa
     2701 accatgcctt ttctgcgcac gcggccgttc atgtctagga cattgccttc gcaacgcact
     2761 actgggatgt gatacccagg caaatcccgt ttatcgacaa tggccttgcc gttgagccta
     2821 aaccactgca caacacagcg cgtcgtggga cgctcattga ctaccttaac ccccgcagcg
     2881 gccatgactt caggcgagta cagctcggat ttgtactttg tcgccccatc gctcaactga
     2941 cacagcgtat cccgcacctc atggatgcgg taatactcgg ctaggcggat ctcttcctta
     3001 ctctcccagt ccaaagtcat gtcgccggga gcatctacat agcgccaatc gctattctca
     3061 gctctgggat actttctctt gtactccgcg cgcttcatcg tctcggaaat gatgcaccag
     3121 ccctgatcca atcccgctgg atgcttggcc gtagggtcca tatagaccgt gaagggattg
     3181 cgaatcggcg caatcttcag ttcctggtca aaactcgtag gagagatgta ttccgacaga
     3241 atgcgccagt agccccaccc gatatctact gcactggata cccccacgtc ataggccacc
     3301 gaggcatcgg agagcgtctc aatgtgccgg atcaggccat tgacgacctc ggcagtatcc
     3361 acatcagccc catccccaac aggatggcat ttgatcctgg gacgctgctg cttgagggtg
     3421 ttcttgagcc ggctaacctg cacattggta tgattgatcg ttaaagcagg ccgaccctct
     3481 atttt
//
LOCUS       DATIPF010000111         2619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1169599, whole genome shotgun sequence.
ACCESSION   DATIPF010000111 DATIPF010000000
VERSION     DATIPF010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169599"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VLL06_02740"
     CDS             <1..409
                     /locus_tag="VLL06_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29914.1"
                     /translation="NLIKASKIPYTIVQSTQFYEFVGAIAQFSTVEQTVHLPPALSQP
                     IGSDDVAAIMADVAVGTPVNGTIEIAGPERIPLDKLVGRFLTATHDPRKVVTDTHARY
                     FGIGVNDQSLTPGSNARIGSTRFEHWLSSMPRT"
     gene            866..1333
                     /locus_tag="VLL06_02745"
     CDS             866..1333
                     /locus_tag="VLL06_02745"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HSS29915.1"
                     /translation="MPKQDVIQRAKKKAREGRAPSTQAGEFVREEMEHIRQGKHGARS
                     TKQAIAIGLSKARKAGVALPPRGKKGTTTGKTRRRSQSRPSARRSRATLDVLKHEPSS
                     AASHQALSTQARRIARQRGSSARHRTAMKAVRTKGPKMLREEARKAAKTRRVR"
     gene            1346..1588
                     /locus_tag="VLL06_02750"
     CDS             1346..1588
                     /locus_tag="VLL06_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29916.1"
                     /translation="MNQAISKATMSVPELALIAGTRAILGVGLGLLLADRLNLDQRKA
                     VGWTMFAIGAISTIPLGFEIFGERLQSGKDQLRTAV"
     gene            complement(1660..2205)
                     /locus_tag="VLL06_02755"
     CDS             complement(1660..2205)
                     /locus_tag="VLL06_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HSS29917.1"
                     /translation="MNKSPADVNAVLCVRDDDHSTGPDTAMITVVAYCDFACPYCGRA
                     YPVIKRLRARLGDRLRFVFRHFPLIDKHPLAQQAAEASEAADAQDQFWPMYDLLFEHQ
                     QALEKKDLTVYAEYLDLDIERFNRALAGRVFQERVDWDIANGQRIGVHGTPTFFINGS
                     QHTDEDTLEDLVLRMSERGPK"
     gene            complement(2274..>2619)
                     /locus_tag="VLL06_02760"
     CDS             complement(2274..>2619)
                     /locus_tag="VLL06_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29918.1"
                     /translation="AEDLARQVAGVRAVNNNLQIQSLADTMTPEGAASQRVEGIKGEV
                     SRIEGDTYFVKGESGKEVRLHTDATTQMTGTIHQGDRIEAQMNDQNHALSIRSTPTTD
                     RRNEKSESMLAQ"
BASE COUNT          619 a          741 c          709 g          550 t
ORIGIN      
        1 aaacctgatc aaggcgtcaa agattcccta tacgatcgtc cagtcaacac agttctacga
       61 attcgtgggc gccatcgctc aatttagcac cgtcgagcaa acggtccacc tgccacccgc
      121 tctttcgcag cccatcgggt ccgatgacgt ggctgccata atggccgacg ttgcagtcgg
      181 gacgccggtg aacggcacca tcgaaatagc cggtcccgaa cggatacctc tcgacaaact
      241 cgtcggacga ttcttgaccg caacccacga tccgcgcaag gtcgtcacag atacccacgc
      301 acgctacttc ggcataggtg tgaacgatca atcgctcaca ccgggcagca acgcgcgcat
      361 cggctcgaca cgttttgagc actggctcag ctctatgccc aggacgtgag gggaaagggg
      421 atgtgtaggg tgttgcctag tagcacattg tgaaaccttg aaggaaaatg tcgacagtga
      481 ggcataggaa atcgctgctg tacgatgacc gttacgaaca agaaatcggg tagtgccctc
      541 tcgaggcgta cgcgatactg gtctgctgaa gtcctaaaaa agagtgacgc gctcgatctc
      601 gagcgaggac tgtttaccct tcagagccca cgcaggattg ccgtatcgct aaagcattca
      661 gctgaacgga gcagacgccg gaagagcacg ccgtatcgat cggcgatgtc gatgctgaat
      721 ttttacatta atcgagccgg caagaggtta agccccccgc agaagagaaa gttggagcga
      781 gcgaaacaag aactgctgac cttgttcaag aaaaccacgt cacgcaggag acatggatcg
      841 tattgaagaa taaaaggagg actcgatgcc gaaacaggac gtgattcaac gcgcgaaaaa
      901 aaaggctcgc gaagggcgag ccccaagcac tcaagctggg gaatttgtcc gtgaggagat
      961 ggagcacatc cggcaaggaa agcatggcgc acgttcgacc aagcaggcca ttgcgattgg
     1021 gctatccaaa gccaggaagg ctggggtggc tctaccgccg cgcggcaaaa aagggaccac
     1081 gacgggaaag acacgtcgcc gttcccaaag tcggccttcc gcccggcgat ccagagcgac
     1141 tcttgatgtc ctgaagcatg agccctcttc tgccgcctcc catcaggcac tgtccaccca
     1201 ggctcgtcgt atcgctcgtc agcgtggttc cagtgcgcga catcgaactg cgatgaaggc
     1261 agtccgaacc aaaggaccaa agatgcttcg agaggaagcc aggaaggccg ccaaaaccag
     1321 acgagtcaga tgaaagggtg gaacaatgaa tcaggcaatc tccaaagcga ctatgagtgt
     1381 gccggaactc gctctgatcg cggggactcg agcgatcctc ggcgtaggcc tcgggcttct
     1441 cctcgctgat cgcctgaacc tagatcaacg caaggctgtg ggatggacaa tgtttgccat
     1501 tggggcaatc agcaccatcc ctctgggatt tgagatcttt ggagagcgat tgcaatccgg
     1561 caaggatcaa ctgcgaacag cagtataagc tttccttttt cgaccgttaa ggccgacacc
     1621 cagcggatgc ttatgtgggg atgacccctc tcgtgcaggt catttgggcc ctcgttcact
     1681 catgcgcaac accagatctt ccagcgtatc ctcatccgta tgctgtgaac catttatgaa
     1741 aaaagtcggc gtgccatgca cgccgatgcg ctggccattc gcaatatccc agtccacccg
     1801 ctcttggaag acccggcccg ctaatgctcg attaaaccgc tcgatatcga gatcaagata
     1861 ttctgcatag acagtcaaat cctttttttc cagagcctgt tggtgttcga agagcagatc
     1921 gtacataggc caaaactgat cctgagcatc agccgcctcg ctcgcttcag ctgcctgctg
     1981 agccaaggga tgcttatcaa tcagtgggaa atggcgaaac acgaaccgca gtcgatctcc
     2041 gagtcgagcc ctgagccgtt tgatgacagg atacgcacgc ccgcagtagg ggcaggcaaa
     2101 atcgcagtag gccaccaccg taatcatggc ggtatcgggc cctgtactat ggtcatcgtc
     2161 tcgtacgcat aacaccgcgt tcacatctgc cggggacttg ttcatgggtg ttcgctcatc
     2221 gccgtgtcga cgatgtttcc agatatgcgc gtcccgtcaa tccgcgtgcg attctactgg
     2281 gccagcatcg actccgactt ttcatttcgg cggtctgtcg tgggcgtcga gcgaatcgat
     2341 agcgcatgat tctgatcatt catctgcgcc tcaatacgat ctccttggtg aatggttccg
     2401 gtcatttggg tcgtggcatc ggtatgcaac cgcacttctt ttccgctttc tcctttgacg
     2461 aagtaggtat cgccctcgat gcgcgacact tcgcccttga ttccttccac gcgttgtgat
     2521 gctgcgcctt ccggcgtcat agtgtccgcc agagattgga tttgcagatt gttattcacg
     2581 gctctcactc cagccacctg ccgcgcgaga tcttctgcc
//
LOCUS       DATIPF010000112         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1697890, whole genome shotgun sequence.
ACCESSION   DATIPF010000112 DATIPF010000000
VERSION     DATIPF010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697890"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VLL06_02765"
     CDS             complement(<1..890)
                     /locus_tag="VLL06_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29919.1"
                     /translation="MELRFQPALLQEVIDSFVEKTEREGDPTYYKEFHELADPIYEKF
                     TLDDRESEFKKLYQYLFGTWGFSDIIRDAFNEYPLLKENVGIVLVKGVLKEDQEGVDI
                     LRKWGSVEHEMARSFEEKGLKGVGIKLIPRRFYDPALTRYCRHELLHISDMLDPVFGY
                     DPDTKVGQNPGEETLILHRYRILWSLTVDSRLTAAGKEPMLRKEDRFKEFRSWYRKIP
                     APQLKSVFEGLWQTSFFTHSELIEMASDTLRVMDRAVDVEGGEVPETENKVMLMPGFP
                     CPLCRFPTYSWVEDMENKIET"
     gene            1106..2209
                     /locus_tag="VLL06_02770"
     CDS             1106..2209
                     /locus_tag="VLL06_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HSS29920.1"
                     /translation="MADPIVSQHGPAPSLLTPLTPGTGSAPVDFLEYWKTAARELTTF
                     RGHSHGVWSVAFAPDCLTLASGGVDRLVRLWDIETGRLLRSLRGHTADIRSVVFTPDG
                     QTLATASEDRTIRLWNPNTGESKKLLFTRYDHNVVNLSLSPDGLMLARGSHNKDIKIW
                     EVTTGTELMTLLGKDQYDHHWSVCVAFSPDGVHLASGMDIGKIKLWEVLPSGEEKIVH
                     DGHWKQTEEDSSETRGYFFDDDGGFQKPMDYWIGAMTFTPDAKLLITGSRDQTIKLFD
                     MPHVAEQRTLKGHTAWVRALAVTPDNKVLISAGDDATIRFWDLSNGRCFRTLKAHTAG
                     VRGITLSPDGLKLASASWDRTVKLWAGGAEPQE"
BASE COUNT          607 a          818 c          690 g          609 t
ORIGIN      
        1 gtctcgatct tgttttccat atcttcgacc catgaatagg ttggaaaccg gcacagagga
       61 cagggaaacc ccggcatcag catcacttta ttttctgtct ccggaacctc acctccctcg
      121 acatccacgg cacggtccat cacgcgcaac gtgtccgacg ccatctcaat cagttccgaa
      181 tgggtaaaga agctcgtctg ccacaaccct tcgaacaccg atttcaattg tggcgcggga
      241 atcttgcgat accaagaacg aaactctttg aaccggtctt ccttcctcaa catcggctct
      301 ttgccggccg cggtcagccg gctatccaca gtgaggctcc acaagattcg atagcgatgg
      361 agaatgagag tttcctcgcc ggggttttgc ccgactttgg tatccggatc gtacccaaat
      421 accggatcca gcatgtccga gatatgcaac aactcatggc ggcaatagcg cgtgagcgca
      481 ggatcgtaga atcgacgagg aatgagttta atcccgacac ctttcaatcc tttttcttcg
      541 aacgatcgag ccatttcatg ctcgacggat ccccactttc tgagaatgtc gacgccttcc
      601 tgatcctctt tgagtacccc cttgacgagc acgattccca cgttctcttt gagtaatggg
      661 tattcgttga aggcatcgcg gataatatcg gaaaaccccc aggtcccaaa cagatactga
      721 tagagtttct tgaactcgct ttcacgatca tcgagcgtaa acttttcata gattgggtcg
      781 gcgagttcgt ggaactcttt ataataggtg ggatcgcctt cccgttccgt tttttcgacg
      841 aacgaatcaa tgacctcttg caacaatgcg ggctgaaaac gaagttccat actcatcctt
      901 tatcatccag cctggtctcg ccgcatcaca acatccggat gaggagcgca aaccggcttg
      961 tatggccagc ttgacttgat ggcgacgctt ctcttacgat catcccaagt ggacattgat
     1021 tatactgggc ggaccagccg gttggcaatc gagtccgtcg ccgtagtcca cgtgaatgta
     1081 ctctcacttg taaggatgat ggaacatggc cgatccaatt gtgtcccagc atggccccgc
     1141 tccctccctg ctcacgccgt taacgccggg aacgggttct gcaccagtcg atttcctcga
     1201 atactggaag acagcagcac gggagctgac cacgtttcgc ggccattcgc atggagtctg
     1261 gtctgtcgcc ttcgcgccgg actgcttgac cctcgccagt ggcggagtcg atcggctcgt
     1321 gcgactgtgg gacattgaaa ccgggcgcct tctgcgctca ctgaggggac atacggcaga
     1381 catccgttcc gtcgtgttca cgccggatgg ccagaccttg gccactgcca gcgaggaccg
     1441 taccatccga ctgtggaatc cgaacaccgg cgaatcgaag aagctcctct tcacacgcta
     1501 tgaccacaat gtcgtcaacc tctctctttc tcccgacggc ctcatgttgg cgcgcggcag
     1561 ccacaataag gacatcaaaa tctgggaagt cacgaccggc accgaactca tgactctcct
     1621 cggcaaggac cagtacgatc atcactggtc ggtctgcgtc gccttttcac cggatggggt
     1681 tcacctcgcc agtggaatgg atatcggcaa gatcaaactg tgggaggtct tacccagtgg
     1741 agaggaaaaa atcgtgcacg acggtcattg gaaacagacc gaagaagatt ccagcgaaac
     1801 gcgcgggtac ttcttcgacg acgacggggg gtttcaaaaa ccgatggact attggatcgg
     1861 cgcgatgact tttacccccg atgccaagct cctgatcacc ggaagccgtg accagacgat
     1921 caagttgttc gacatgcccc acgtggctga acaacggaca ctgaaaggcc acaccgcctg
     1981 ggtgcgcgcg ctcgccgtca ccccggacaa caaagtgctg atcagtgccg gcgacgatgc
     2041 gacgattcgg ttttgggacc tgtcgaacgg ccgttgcttc agaacactca aggcccacac
     2101 cgcaggcgtt cgaggcatta cgctgtcgcc ggatggactg aaactggcca gcgcgtcctg
     2161 ggaccgcacg gtgaaattgt gggccggtgg cgcggagcct caagagtaac tcgacgaccc
     2221 ccgccattta caccctaaca taaggtccga cggaggtggg gtgacatggc ccttcggctc
     2281 ccaacgcctt cccggaacgg ccctctcgga cgttgccagg cggaatcctt cggaaattcc
     2341 ttcgcagact cagtcagttt ccgctttcct tcctggcaac gtctctatgg gcaccccgtt
     2401 cctccggcga tttacgcttc agggccatgc cacccctgcc agtaagtctt cagctaatta
     2461 aaaatagaaa ggcgacaggc cgcttacaag cctgtcgcct taacgcttcg ccactagata
     2521 cttcacggct gcttcgcacc atcgacaacg gagcatgtcc ctgtttttct ctctaatgcc
     2581 gcactgatac gcgcgggaca cgagcataac aggaaacagt gcgctgtccc atcgcgtctg
     2641 cctcgaaggc gaagttagat gccaccgctc tcatctttgg tcgcctcaag cctttcatca
     2701 tttgcagtaa tccacgcttc tagt
//
LOCUS       DATIPF010000113         8396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_639218, whole genome shotgun sequence.
ACCESSION   DATIPF010000113 DATIPF010000000
VERSION     DATIPF010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639218"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1332)
                     /locus_tag="VLL06_02775"
     CDS             complement(145..1332)
                     /locus_tag="VLL06_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HSS29921.1"
                     /translation="MRYWAGVMIGVVVWGLLSLFLPTGAVADPIIDKIEKERRALDQL
                     KGQIEEKRKQADEAGKKRESLLQGIQTLDERVLRYRQAHQEISRKLRKKDQEIETINA
                     QLADLRVSVRERQDAILARLRVQYMEGRFGHWKTLLASDSYGDFQRRLQYLSAVSERD
                     YALMGMFRTDMTRMEQAERQREDARVGMLSYKQSTEKQLTEMKSVKKEKKVYLTKITH
                     EKESYEHALQELERSASRIDSLLRELEQRRRAAAARPPTGMIAPRGVKGALPWPADGS
                     IISFFGRQKHPTFNTYVQRRGIEIKTVEGSAIHAVMPGSVVYADWLKGYGLVIILDHE
                     NGFFSLYAHASKILTTVGGQVGAGQAIGETGDTGMTGENTLYFELRQGAEPIDPLQWL
                     ARR"
     gene            complement(1329..2228)
                     /gene="ftsX"
                     /locus_tag="VLL06_02780"
     CDS             complement(1329..2228)
                     /gene="ftsX"
                     /locus_tag="VLL06_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HSS29922.1"
                     /translation="MRTLSYLLKESWVNMRTNRTTTMVAILTTAFTLACVGVFLLLYV
                     NLRAAAGWLQDDIKIMVYLDERLASDSVAEVEQHLRADRAVASLLFISKEQALGEFKA
                     QFPSESHLLEGLGQNPLPASFVVALNPAFRSVDAIKRWAERVRQVAGVSKVDYNQDWI
                     DALATVIRSIELVAIGIGVILSAAAVTIIANTIRLTLFARRDEIAILRLIGATTMFIR
                     VPYLMEGAVLGALGSTLSLVMLKFMYELFRQQMRTTGRFAGLDNLIAFFPPSVCLALV
                     AVGMGLGFAGSFVSIRRFGEAKA"
     gene            complement(2225..2893)
                     /gene="ftsE"
                     /locus_tag="VLL06_02785"
     CDS             complement(2225..2893)
                     /gene="ftsE"
                     /locus_tag="VLL06_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HSS29923.1"
                     /translation="MIQLIHVSKQYDQRLALSDVTFEIGKGEFVLLMGPSGAGKSTLL
                     RMLIGAERPDEGQIFVHQKNVTALKSSDVPYLRRKVGTVFQDFRLLPKKSVFDNVALP
                     LLVQGISPADIRRKVTEALRAVGVEHKRDQLPAGLSTGEQQRVCIARAIVNGPVVLLA
                     DEPTGNLDPALTSEIIELFKLINARGTTVVVATHDPLVMAQVNRRVLTLSQGRLLPEQ
                     RVLG"
     gene            complement(2901..3293)
                     /locus_tag="VLL06_02790"
     CDS             complement(2901..3293)
                     /locus_tag="VLL06_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HSS29924.1"
                     /translation="MTFLDPRKLLGQEGESAAEQYLRHKGYRIVARNLRSSMGELDIV
                     AEDGQALVFVEVKARRTDTFGGAIHAVHQRKQEKLIQLAAQYLARHHITNRPCRFDVV
                     LLQSAGAVAPQIEHIQNAFEVSGDDLRW"
     gene            complement(3290..3952)
                     /locus_tag="VLL06_02795"
     CDS             complement(3290..3952)
                     /locus_tag="VLL06_02795"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HSS29925.1"
                     /translation="MGPTDEFELEARRRGYRWIAGLDEAGRGPLAGPVVAAAVILPTR
                     CRLAGVDDSKQVSEPERARLYDVIVQRARSVAIGVATEQEIDHLNILNATRLAMCRAL
                     ALLTLQADYLLIDAVTLPDLAIPSRSIIKGDTLSMSIAAASIVAKVMRDRMMVAYHRA
                     YPEYNFLSHKGYGTEEHLLRLTQHGPCAIHRRTFAPVAQLLYGDGGFSRTGLDAAPRT
                     NP"
     gene            complement(4023..4445)
                     /gene="rplS"
                     /locus_tag="VLL06_02800"
     CDS             complement(4023..4445)
                     /gene="rplS"
                     /locus_tag="VLL06_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HSS29926.1"
                     /translation="MNRLERIQQSFTKKAVPGFEIGDTVRVHVKVVEGEKERIQVFEG
                     AVIARKGLLNTETFTVRKVSYGVGVERIFPLHSPIVTRVEVMRQGKVRRAKLYYLRGK
                     KGKFAKVEDREFVASAKGQPGVTRKVEEPVGAASTSKS"
     gene            complement(4450..5232)
                     /gene="trmD"
                     /locus_tag="VLL06_02805"
     CDS             complement(4450..5232)
                     /gene="trmD"
                     /locus_tag="VLL06_02805"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HSS29927.1"
                     /translation="MRCDILTLFPDMVAPVLGQSILKRAQEKGLLHVQVRNLRDYMLD
                     RHKVADDVPYGGGAGMVMKAEPILRAVEQIQADYAEAGEQIRLLFPSPHGRPFTQAYA
                     KHLAADPRRLVILCGHYEGVDERVRLALHPEEISVGDYVLTGGELPALLLIDAAARLV
                     PGVLGDPESIVEESFTDGLLEYPHYTRPAEVRGMPVPDVLLSGHHEAIRLWRRKEALR
                     STYQRRPDLLQDRVLSLEDQQLLDELTREGVPDMPVRCGGEG"
     gene            complement(5222..5758)
                     /gene="rimM"
                     /locus_tag="VLL06_02810"
     CDS             complement(5222..5758)
                     /gene="rimM"
                     /locus_tag="VLL06_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HSS29928.1"
                     /translation="MTTAHTDLVTIGKIERPFGVRGEFKVRSLSDVPGRLDQLKQVQV
                     LRPTGQMVDRTVTRVRRAGSTYIMGLEGVTTPEEAGMLRGGLIQVPRSPAAMLSADVY
                     FECDLIGMTVENERGEEVGVLETILEIPDNHLFVVRKGKEEVLIPAAKSFVTSVDLTR
                     RRMMVRGIEDLVEDRHAM"
     gene            complement(5762..6046)
                     /gene="rpsP"
                     /locus_tag="VLL06_02815"
     CDS             complement(5762..6046)
                     /gene="rpsP"
                     /locus_tag="VLL06_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HSS29929.1"
                     /translation="MAVHLRLTRTGRHKRPLYCVVAADSRKPRDGRFLEILGIFDPLK
                     EAGLPELKQERVLSWLHQGAQPTVTVKTLLRRSGVWKQFEAEKAAKKKGA"
     gene            complement(6094..7443)
                     /gene="ffh"
                     /locus_tag="VLL06_02820"
     CDS             complement(6094..7443)
                     /gene="ffh"
                     /locus_tag="VLL06_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HSS29930.1"
                     /translation="MLDALSEKFENIFKKLRGSGVLTEQNIAEALKEVRLALLEADVN
                     FKIVKDFIERVREKAVGQEVLRSLTPGHQVVKVVWEELREMMGRERAGLALASNPPTV
                     VMMVGLQGAGKTTTCGKLGRYFKAQGKRVLLVAADPRRPAAGDQLASLGHDLDIDVHR
                     VDQAQASRGDVIRICQAGVERGRDQGHDLVVLDTGGRLHIDDELMGELVAVKAAVQPH
                     EVLLVADAMTGQDAVTMASQFEQRIGLTGVVLTKVEGDARGGAVLSIRAVTGKPIKFL
                     GVGEKLDALELFHPDRMASRILGMGDVLSLIEKAQETITREQVEDAQKRLTSNTFTLE
                     DFRAQLGQVNRLGSLDQILGMLPGGQKLKSAMEGQVAEREMKRVAAIIDSMTLRERRD
                     HTVINGSRKKRIARGSGTNVQEVNRLIKQFLSARKIAKVMAAGSGGRRQFAQLMRSR"
     gene            7646..7906
                     /locus_tag="VLL06_02825"
     CDS             7646..7906
                     /locus_tag="VLL06_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HSS29931.1"
                     /translation="MKKSPWVLLIFVLIGGLLGGVLGEILRVMAPQGTIQSIFATSFT
                     PGISPPLTIDLVLIKLTMGFALKINLLSFLGMFLGIYLYKNI"
     gene            complement(7914..>8396)
                     /locus_tag="VLL06_02830"
     CDS             complement(7914..>8396)
                     /locus_tag="VLL06_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HSS29932.1"
                     /translation="GLDLPEVTLVAILDADKEGYLRSYRALIQTAGRAARNVRGHVIM
                     YGDAVTNSMQMALAETGRRRTIQADYNARHGIVPTSIKKDVLSLEYATADMDDVQLEL
                     AAEAPAIYRAEDTTEQTIKRLETEMKAAAKALEFERAAILRNRIRALKLKELELKPEI
                     "
BASE COUNT         1860 a         2642 c         2044 g         1850 t
ORIGIN      
        1 ggtaatcctg ggtcatcgaa gcgccggggt cgggtgggtg agaccagcgg cttttttgaa
       61 catcctaccg gcgtattctc tttctgtcca aacatgtcag ctattataag ctctgcgatg
      121 ccggatcggt ttttcgaaag cctgctagcg cctcgccaac cattggagag gatcaatcgg
      181 ctccgccccc tggcgtaact caaagtatag agtattttca cccgtcatgc ccgtgtctcc
      241 tgtttcgccg atcgcttgcc ccgctcccac ctgccctccc acagtcgtca gtatcttcga
      301 agcatgggca tacagcgaaa agaacccatt ctcatgatca aggattataa cgagtccgta
      361 ccctttcaac cagtctgcat agacaacgga cccaggcatc acggcgtgaa tagcactccc
      421 ctcaaccgtc ttaatttcaa tacccctgcg ctgcacatag gtgttgaaag tcggatgttt
      481 ctgacggccg aagaaggaga tgatgctccc atcggccggc cacggcagtg cacccttcac
      541 ccctcggggg gcgatcatcc cggtcggagg tctggccgca gccgcgcgac gacgctgctc
      601 caactcgcgc aacagactgt cgatgcgcga cgcggaccgt tctaattctt ggagggcatg
      661 ttcgtacgac tctttttcat gagtgatttt agtaagatag accttctttt ctttcttgac
      721 tgatttcatc tcagtcaact gcttttcggt actctgcttg tatgacagca tcccgactcg
      781 cgcgtcttcc cgctgccgct ccgcctgctc catacgggtc atatcggtcc ggaacatacc
      841 catgagggca tagtcccgtt cggatacggc agagagatac tggagccggc gttggaaatc
      901 accatacgag tccgaggcaa gcaaggtttt ccaatgccca aaacggccct ccatgtactg
      961 aactcgcagc cgcgccagaa tggcgtcctg gcgttctcgt acactcacgc gcaagtcggc
     1021 gagttgcgca ttgatggtct cgatctcttg gtctttcttt cgcaacttcc ggctaatttc
     1081 ctgatgcgct tgtcgatagc gcaacacacg ttcgtcgagt gtctgaatcc cctgcaagag
     1141 ggactctcgc tttttccccg cttcatcggc ctgtttgcgt ttttcttcga tctggccttt
     1201 gagttggtcg agcgcccggc gttctttctc aatcttgtcg atgatcggat cggcaaccgc
     1261 ccccgtcggc aagaaaagac tcaacaaccc ccagaccacg actccgatca tcaccccagc
     1321 ccaatacctc atgccttggc ctcgccgaaa cgccgtatcg acacgaagct gccagcaaac
     1381 ccgagcccca tccccaccgc aaccagtgca aggcagactg acggagggaa aaatgcaatg
     1441 aggttgtcga gcccggcaaa tcgtcccgtc gttcgcattt gctgccgaaa caactcatac
     1501 atgaacttga gcatgacgag tgagagcgta ctgcccaagg cgccaagcac cgcgccctcc
     1561 atgaggtatg gaacacgaat aaacatggtg gtcgctccga tgagacgcag gatggcaatt
     1621 tcatcccgtc gagcgaataa tgtgaggcga atcgtattgg caataatggt gacggccgca
     1681 gccgagagaa tcacaccgat gccgatggcc actaactcaa tcgatcgaat caccgtcgcc
     1741 agggcgtcga tccaatcttg attatagtcg accttggaga caccggcgac ctgcctgacc
     1801 cgctctgccc atcgcttgat tgcatccacc gaacggaacg ccgggttcag tgccacgaca
     1861 aaggaggccg gtaaaggatt ctggcccaac ccctcaagca agtgagactc cgaaggaaat
     1921 tgtgccttga actcccccaa tgcctgctct ttcgagataa acaacagcga agcgactgcc
     1981 cggtcggccc gcaaatgctg ctccacttca gcgaccgaat cggaggcgag ccgctcatca
     2041 agatacacca tgatttttat atcgtcctgg agccatcccg ccgccgcccg caaattgacg
     2101 tagagcagca agaaaacgcc gacacaggcc aaggtaaagg ccgtagtcaa gatggcgacc
     2161 atcgtcgttg tccggttggt ccgcatattg acccaggatt ctttgaggag atacgagagc
     2221 gtcctcaccc cagcacccgc tgttccggta gcaaccggcc ctgtgacagt gtgagcaccc
     2281 gccgattcac ttgcgccatg accaggggat catgcgttgc caccacgaca gtagtgccgc
     2341 gtgcgttaat gagtttaaac aattcgataa tctcggacgt cagggctgga tccaaatttc
     2401 cggtcggctc atccgccagc aagacgacag gaccatttac aattgcacgt gcaatacaga
     2461 cccgctgctg ctcgcctgtc gagaggcccg caggcaactg atctcgctta tgctcgacac
     2521 cgaccgcccg taatgcctcc gtgactttcc gacgaatgtc agcgggagaa atgccttgca
     2581 ccagcaaggg caacgccaca ttatcgaaga cggacttctt cggcaacagc cgaaagtctt
     2641 gaaacaccgt tcccaccttg cggcgcagat acggaacgtc agacgacttg agcgctgtca
     2701 cgtttttctg atggacgaag atctgcccct catcgggccg ttcagccccg atgagcatcc
     2761 gtaacaacgt ggacttgccc gcgccgctcg gtcccatgag gaggacaaat tctcccttac
     2821 cgatctcgaa ggtgacgtcc gagagcgcca gccgctgatc gtactgcttc gatacatgga
     2881 tcaattgaat cattgtccct ctaccaacga agatcgtctc cggacacttc gaaggcgttt
     2941 tgaatatgct cgatctgcgg ggcaacggca cccgcactct gcagcaagac gacatcgaat
     3001 ctgcatggtc gatttgtaat gtgatggcgg gccaaatact gagccgctag ctgaatcaac
     3061 ttctcctgct tccgttgatg gacggcgtgg atggctccgc cgaatgtgtc ggttcgacgc
     3121 gccttcacct cgacaaagac taacgcctga ccatcctcag ccactatgtc cagctccccc
     3181 atcgacgaac gcagattgcg tgccacaatc cgatacccct tgtgccgcag atattgctcc
     3241 gccgctgatt ctccttcttg ccctaaaagc ttccggggat caaggaaggt cacggattcg
     3301 tccttggagc tgcgtccaat ccagtccgtg agaacccccc atcgccatat aagagctgtg
     3361 cgactggagc gaatgtacgc cgatgaatcg cgcaaggacc atgttgagtg agccgtagta
     3421 aatgctcttc ggtcccatac cccttgtgtg agagaaaatt atactcagga taggctcggt
     3481 gataggccac catcatgcga tcgcgcatga ctttggccac aatcgacgca gccgcaatcg
     3541 acatcgacaa cgtatcaccc ttaatgatgg agcgtgaagg aatggcgaga tccggcaatg
     3601 tcacggcatc gatcaagagg tagtccgcct ggagagtcag caatgccagc gcacgacaca
     3661 tggccaggcg agtcgcgttt aaaatattga ggtggtcgat ctcctgctcc gtggccactc
     3721 ctatagcgac actgcgtgcg cgctggacga tcacatcgta gagtcgggct cgttcaggtt
     3781 cggaaacttg cttggaatcg tcgacaccgg ctaagcggca acgagtgggg aggatcacgg
     3841 ccgccgcgac gacagggccc gccagagggc cccgtccggc ttcatcaagg ccggcaatcc
     3901 aacgatagcc gcgccgccgg gcttccaatt caaattcgtc ggtaggtccc acgatgtggc
     3961 cccgcgatgc atccgttgaa agggcacctg acactcgtgg tcgatggaac cagagaatgg
     4021 gattaggact tcgatgtcga ggcggctccg accggctctt cgactttacg ggtgacccca
     4081 ggttgcccct ttgcactcgc gacgaattcc cgatcttcga ccttggcaaa cttgcccttc
     4141 ttcccgcgga gataatacag cttggcccga cgcaccttgc cctgacgcat gacttccaca
     4201 cgcgtgacga tcggcgaatg gagtgggaaa attcgttcca cgccgacgcc gtaggaaact
     4261 ttgcgcacag tgaaggtctc cgtgttcagc aaccctttgc gagcgatcac cgccccttcg
     4321 aacacttgaa tgcgctcttt ttccccttcg accactttca cgtgcacgcg aaccgtatct
     4381 ccaatttcaa aaccgggcac cgccttcttt gtgaaagact gttgtatccg ctccaaccga
     4441 ttcatgaaac tacccctccc ccccacatcg aacaggcatg tccggcacgc cttcacgagt
     4501 aagttcgtct agcaattgtt gatcctcgag actcaagacc cgatcttgca aaagatccgg
     4561 ccgtctctga tacgtgcttc gcagggcttc cttacgacgc cacagacgaa tggcttcgtg
     4621 atgtcctgaa agcaacacgt ctggcacagg catacctcga acttccgccg gtcttgtata
     4681 gtgtggatat tcgagcaacc catccgtgaa cgactcttct acgattgact ccgggtcacc
     4741 caatacaccg ggaaccaaac gggctgcagc atcgatgagc agcagagctg gtaattcgcc
     4801 tccggtcaac acataatccc cgacagaaat ttcttccggg tgcaatgcca accgcacccg
     4861 ttcatcgacc ccctcatagt gaccacacag aataacgagt cgacgtggat cggcggccaa
     4921 atgtttcgca taggcctgtg tgaacggacg accgtgcgga gacggaaaca gcaaccgaat
     4981 ctgttcacca gcctctgcat aatccgcctg aatctgttcc accgcgcgca agatgggctc
     5041 agccttcatc accattccgg cccctccgcc atagggcacg tcgtccgcca ccttgtggcg
     5101 atcgagcata tagtcacgca aattgcgcac ctgcacatgg agcagacctt tttcctgtgc
     5161 tcgcttgaga atactctggc ccagcaccgg cgctaccatg tcaggaaaca acgtcagaat
     5221 gtcacatcgc atggcgatcc tcaaccaagt cttcaatccc gcggaccatc atccggcgac
     5281 gcgtgagatc caccgaggtc acaaagctct tcgcggccgg aatgaggact tcttccttcc
     5341 ccttgcggac gacgaagagg tgattgtccg gtatctctaa aatcgtctca agcacaccca
     5401 cttcttcgcc ccgttcgttc tcaaccgtca tcccaatgag atcacattca aaatagacat
     5461 ccgccgacag catagcagct ggactgcgtg gcacttgaat caatccccca cgcagcatcc
     5521 cggcttcctc cggcgtcgtc acaccctcaa gacccatgat gtaggtcgag ccggcacgcc
     5581 gaacacgagt caccgtcctg tcgaccatct gtcccgtcgg tcgtaaaacc tgaacctgct
     5641 tcagttgatc cagccggcca ggcacatcgc ttaacgatcg caccttgaat tcgccccgca
     5701 cgccaaaggg tcgctcgatc ttgccaatcg tgacaagatc tgtgtgagcg gttgtcacgg
     5761 actaggcgcc cttcttcttc gccgctttct ctgcttcaaa ctgtttccat acgccactgc
     5821 ggcggagcaa cgtcttgacg gtgacggtcg gctgagctcc ctggtgcaac cagctcaaca
     5881 cccgttcctg cttcagctcc ggcagacccg cctctttcaa aggatcgaaa atcccaagaa
     5941 tttccaggaa ccgcccgtcg cgcggcttcc gagaatccgc tgccacgaca caatagagcg
     6001 gtcgcttgtg tcgtcctgtc ctcgtcaatc tcaaatgaac agccactaga ttcctcctcc
     6061 tgtacagaca cccatgatga tcgttttcga ctcttatctg gagcgcatca gttgtgcgaa
     6121 ctgccggcgc cctccagacc ctgctgccat aaccttcgcg atttttctcg ccgagagaaa
     6181 ctgtttgatc aaccgattca cctcttgcac gttcgtccca cttccacgcg cgatccgctt
     6241 ttttcggctt ccattgatga ccgtatgatc gcgccgctct cgcaaggtca tcgagtcaat
     6301 aatcgccgcg actcgtttca tctctcgctc cgctacctgc ccttccatcg cactcttgag
     6361 cttctgaccg ccgggcaaca tccctaaaat ctggtccaac gaacccagac ggttgacctg
     6421 acccaattga gcacggaagt cctccaacgt gaacgtatta ctagtaagtc gtttctgcgc
     6481 atcctccacc tgttcgcgcg taatggtttc ttgggccttt tcgatcaacg agagcacgtc
     6541 acccattccg aggatgcgcg aagccatccg atctggatga aacaactcaa gtgcatcaag
     6601 cttctcgccc acacccagga acttgattgg cttgccggtc acggcccgga tagacagcac
     6661 cgctccgcca cgggcatcac cttcaacctt ggtgagaacg acaccggtca gtccgatccg
     6721 ctgctcgaac tggctcgcca tcgtgaccgc gtcttgcccc gtcatggcat ctgccaccaa
     6781 gagcacctca tgcggttgca cggcagcctt cactgccacc aactccccca tcaactcatc
     6841 atcgatatgg agacgcccac cggtatccaa gacgacaaga tcgtgcccct ggtcccgtcc
     6901 ccgttctacg cctgcctgac agatgcgaat gacatcccct cgggaagcct gcgcctgatc
     6961 gactcgatgc acatcgatgt caagatcgtg tcccaaactc gccaactgat cgccggctgc
     7021 aggacgacgt ggatctgcag caaccaagag cacccgtttc ccttgagcct tgaaataccg
     7081 cccaagcttg ccacaggtcg ttgtctttcc agcaccttgg agccccacca tcatgacaac
     7141 cgttggggga ttcgaagcca gagctaggcc agctcgctcc cggcccatca tctcccgcag
     7201 ctcctcccac acaaccttca cgacctgatg accaggggtt aagctccgga ggacttcctg
     7261 accgactgcc ttttcgcgca ctcgctcaat gaaatccttg acgatcttga agttcacatc
     7321 ggcttcgagc agcgcgaggc gtacttcttt caacgcctcc gcgatgttct gttccgtgag
     7381 gactccagag ccgcgtagct tcttgaaaat gttctcgaat ttttcgctaa gagcgtccag
     7441 catagcgcca caaagaaaaa ggcaatcgaa gtcttaactc cagatctccg atcgcctcga
     7501 aatctctagt aaaagaccac cgaggagtct agaggatggg acaggatgaa gtcaagagaa
     7561 ccagagcgca catttcattg acatgtcgta gacctttcgt tatggtccgt cgtgtgtata
     7621 cgcagagagc tgtaaggcag ttgctgtgaa aaaatcaccg tgggttctgc tcatctttgt
     7681 gctcatcggc ggactgctgg gcggagtact gggagaaatc cttcgggtca tggcccccca
     7741 gggcacaatt caatcgattt tcgccaccag ttttaccccc ggcatcagcc caccactgac
     7801 gatcgatctg gtcctgatca aactcaccat gggcttcgcg ctcaaaatta atctcctcag
     7861 cttccttggg atgtttcttg gcatctatct ttacaagaac atctaaccag ctgctagatt
     7921 tccggtttca attctaactc cttgagcttc aatgcccgaa tccggtttct gagaatcgcg
     7981 gcccgttcaa attccaacgc cttggcggcg gctttcattt ccgtttccag ccgtttgatg
     8041 gtctgttcag tcgtgtcctc ggctctataa atggctggtg cttccgcagc caactccagt
     8101 tgcacatcgt ccatatccgc tgtggcatat tcaagtgaca ggacatcctt tttgatgctc
     8161 gtagggacga taccgtgtct ggcattatag tcggcctgaa tcgtccgccg acgaccggtc
     8221 tcggcaagtg ccatctgcat agaattggtg acggcatcac cgtacataat tacatggccc
     8281 cgcacattcc tcgctgcccg tccagccgtc tggatcaatg ctcgatacga ccgcagatac
     8341 ccctccttat ccgcatcgag aatcgccacg agggtcactt cggggagatc cagccc
//
LOCUS       DATIPF010000114         5147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1612815, whole genome shotgun sequence.
ACCESSION   DATIPF010000114 DATIPF010000000
VERSION     DATIPF010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612815"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="VLL06_02835"
     CDS             complement(<1..1299)
                     /locus_tag="VLL06_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HSS29933.1"
                     /translation="MSDSVIITFALIAAVTGIAYGLYLAMWVFKLDAGNPKMQDIAKA
                     IQEGAAAYLNRQYRTVGVVAAVLFVILWGAGAFSDKFGLLTAIGFLIGAGASSLAGYV
                     GMVIAVRANVRTAQAAHKGMNAALTVAFRGGAVTGLLLIGLGLLAITTFYTLASQFAG
                     QEHAIHALLSLGFGGSLISVFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVI
                     ADNVGDNVGDCAGMAADLFETYAVTTVAAMVLAFTLFKGVSAPILYPLALGGVTIFAT
                     IIGIFFVRVSEGGEIMTALYKGLFVAGGIAAIAFYPVTSTIMDGVGGVSGVSYFIAAL
                     IGLAVTLGLVFITDYYTSKSYKPVKAIAKASETGHATNIIAGLAVGMEATAWPVVVIG
                     AAILSSYWICGGAASGGLYGVAVAAVSMLSMAGIVVAIDAF"
     gene            1817..2976
                     /locus_tag="VLL06_02840"
     CDS             join(1817..2069,2069..2976)
                     /locus_tag="VLL06_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611297.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HSS29934.1"
                     /translation="MGNSRRSFTPEFKQEAVDLCRRSGKSECQVARELGIPQPTFNRW
                     MRQVAGAPLGSNRFVATEELKVLRREVEQLRMERDILKKAGGLLCQGVGMRYRCIQAE
                     KASYPVEALCRVLTVARSGYYAWCQRPLGARAQQNQGLLTQIRTCYQAAHGRYGSPRI
                     HQDLQARGIRVGRHRVARLMRLQGLRSIRRRRAWRAPGMTAPGVVARNILRREFAATQ
                     PNEKWAGDITYVPTREGWLYLAVLMDLYSRRIVGWAMDDRGATSLTLSALEMALRHRA
                     MRADLLHHSDRGSQYGALAYQQRLAIRGIRCSMSRPGNCWDNAVVESFFATLKTELCS
                     KGHPFSTRQEARTAIFEYVEGFYNRTRRHSTLGYLSPTEFEQRAAYSTTGVH"
     gene            complement(4076..4774)
                     /locus_tag="VLL06_02845"
     CDS             complement(4076..4774)
                     /locus_tag="VLL06_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29935.1"
                     /translation="MQISIITLSLIFSILGISPVEAGELPAREPLDALWKQHMLEGDA
                     HTSVHIEINGGIFHKSERAAKEPSGSWKGGRWNMFFNESRNITVAQYGEGFWGKGGWV
                     LEGGLVGRAKEGSSTIELYGKAVEHKLPYTQFLIDRNIPHESGGYEFYLLQREGSCPR
                     ATIVRKWTIPPHEVVLKPQSVGPPEEDVRATLEYDDTAKRATIGITGLTRPFKESVAI
                     SEREGTKPEQLETR"
BASE COUNT         1155 a         1470 c         1404 g         1118 t
ORIGIN      
        1 gaacgcatcg attgccacca cgataccggc catcgacaac attgagactg ccgccacggc
       61 cacgccatat aagccacccg atgccgcgcc gccgcagatc cagtagctcg aaagaatcgc
      121 cgctccgatc accaccaccg gccaagccgt cgcttccatt cccactgcaa gaccagcaat
      181 gatgttggtg gcatggcccg tttcacttgc cttggcaatg gccttcacgg gcttataact
      241 ctttgaagta tagtaatcgg tgataaagac gaggccgagg gtcacggcca agccgatcaa
      301 cgccgcgata aaatagctca ccccgctcac gccacccacc ccatccatga tggtgctcgt
      361 cacaggatag aacgcaatcg cggcaatacc gcccgccaca aacaaacctt tataaagcgc
      421 cgtcatgatt tcgccaccct cgctcaccct cacgaagaaa atacctatga tcgtcgcgaa
      481 gatcgtcacg cccccgagag cgagcggata gagaatcgga gcactgactc ccttgaagag
      541 ggtaaaggcc aagaccatcg ctgccaccgt cgtcaccgca taggtttcga aaaggtccgc
      601 agccataccg gcacagtcgc cgacattgtc gcccacgtta tccgcaataa cagcagggtt
      661 gcggggatcg tcttccggaa tacccgcttc aaccttccca acgaggtctg cgccgacgtc
      721 cgccgctttc gtgtagatac caccgcccac acgggcaaat accgagatca aactcccgcc
      781 gaacccaaga ctcagcaacg catgaatcgc atgttcctgt cccgcaaact gggatgccag
      841 cgtgtagaac gtggtgatgg ccagcaagcc caagccgatc aacaggagcc ccgtcaccgc
      901 accgccacgg aacgccacgg tcagtgccgc gttcatcccc ttatgggcag cctgggccgt
      961 ccgaacgttc gcccgtaccg cgataaccat gcccacatac ccggccaacg acgaggcacc
     1021 ggcgccgatc aagaatccga tcgcggtcag taggccgaat ttatcggaga aagcccccgc
     1081 gccccacaag ataacgaaca acaccgcagc gacgacgccg acagtccggt actgccggtt
     1141 cagataggca gccgcgcctt cctgaatggc cttcgcaata tcctgcatct tcgggtttcc
     1201 ggcgtcgagc ttgaataccc acatggccaa atacaggcca taggcgatgc cggtcaccgc
     1261 cgctatcaac gcaaacgtaa tgatcaccga gtcactcact gtactctcct cccgttatca
     1321 gaatggtaca aatggtccag cattgtgccc ttgtccaccg gcctggcagg ccggtccgca
     1381 ggcagacagg tggccacgct ggagagccat tttgaccatc atgcaaaagt gctcatccca
     1441 gctctatgtg aaactgcaac cccgtgatgt atgtcacaca actccccgag gcttcaaccc
     1501 atccaaagta catgagtcgg aaggacgaaa cgcgaggtgg tgcagattcg atccgtgcag
     1561 taactactcg aaaagttggg ccattctata caggtgacga gagcggatca agataaaaga
     1621 cagagtaatc tcgttttacc atcagcacac tttctggcta gacagacggt ttaggaaacc
     1681 gtagacgcta ccctcctcag gctcactcga actcactcag gcctactgca gccaatgttt
     1741 gcgcgaaact caaactttgg tcttcccctg attttgtgga caccttccgg gcataatcag
     1801 cgaagggagg tgcgtgatgg gcaactcacg tcggagtttc accccggagt ttaagcaaga
     1861 agcggtggat ttgtgtcggc gtagcgggaa gagcgagtgt caggtggcgc gggaattagg
     1921 cattccgcaa ccgacgttca atcgatggat gcggcaagtc gcgggcgcgc cgctcgggtc
     1981 caaccgcttc gtggcgaccg aagaactgaa ggtcttgcgc cgggaagtag agcaacttcg
     2041 catggagcgc gacatcctaa aaaaagccgt ggccttcttt gccaaggagt cgggatgagg
     2101 tatcgctgca ttcaggcaga gaaggcctcg tatcccgtgg aggccttgtg ccgcgtgctc
     2161 accgtggccc ggagtggtta ttacgcctgg tgccagcgac cgcttggggc acgcgcgcag
     2221 cagaatcaag ggctgctcac gcagattcgg acctgctatc aggcggcaca tggtcggtat
     2281 gggagtccgc ggatccatca ggatctgcag gctcgtggga tccgggtggg ccgtcatcgc
     2341 gtcgcgcgat tgatgcggct tcagggactg cgcagtatcc gtcggcggcg ggcctggaga
     2401 gcaccaggca tgacggctcc tggcgttgtc gccaggaaca ttttgcggcg agagtttgcc
     2461 gcgacccagc cgaatgagaa atgggcgggc gatatcacct acgtgcccac ccgtgagggc
     2521 tggctctacc tagcggtgct gatggatttg tactcgcgtc gtatagtcgg ctgggcgatg
     2581 gatgaccgtg gagccacgag cttgacgctc agtgccttgg agatggcgct gcggcaccgc
     2641 gccatgaggg ctgacctgct ccatcattcg gatcgaggga gccagtacgg ggccctggcg
     2701 tatcagcagc ggttagcgat tcgcgggatt cggtgctcga tgagccggcc cggcaattgt
     2761 tgggacaatg ccgtggtgga gagtttcttt gccacgctga aaacggaatt gtgctccaag
     2821 gggcacccgt tttcgactcg gcaggaagcc cgaaccgcga tctttgagta cgtggaggga
     2881 ttttacaatc ggacccgccg gcattccacg ttgggctacc tcagtcctac ggagttcgaa
     2941 cagcgggccg cttactcaac caccggtgtc cactgaatcg ggggaagacc aaactctcga
     3001 actttggctg gaataggcct gagtgggctg gaatgaggtg ggcttgagca caaaacgagc
     3061 acaattggct ggggtgaacc cctccggtga gacacgaaaa ttagcacact cgtgtctcaa
     3121 tctagggggg tcagtccaca gctttctgca gtggacgatc ttcgcaagcg gggcaaatca
     3181 accaactaga gccaggccgt tgctctcaac tcacattttt atagccgctc ggtcgctaag
     3241 gaggtaccgt gaggagacca atggagagga agaggggtac gctgatgctg catattcggc
     3301 tcagacattg gatcgttagc gcaggtattc ttgtgttgtt cttcgtgcca agcaactcgt
     3361 ttgggactgg taatgaggag gtggtttacg agtttgcccc acacttcaag ggctgggttc
     3421 tcattcagtg ggaagatccc gcatgtgtag cattaccatc aaaaccgggg agtattcata
     3481 tcccgattcc cgcgtctggg tgtgcttgca cgagcgaccc tctaccgctt ggatttcggc
     3541 cgaatcgata tgagcgtatt cattcggacg gcacaaaaga ggttttaccg tatcgactta
     3601 atgacgacag ctctgagata aggagtcata tctcagggat ggggccttcc agggcggtcc
     3661 acaagccact acttcgcgaa gcattttttg tgggcagtaa gaaggagtat atgagccaat
     3721 acaagctgaa tcccctcggt cctcagtttc ccgagcagaa gatttgtacg gaattagatg
     3781 atcacgcccg tctgaaatag atagtgtggg ttgttcagca actgttctag cggccctgcc
     3841 aaattccatt cctttgcctg aatcccgatt aagcgaaagg tttccccaga caatactccc
     3901 ccctctcatt gatgcaactg acagaggagt ttctgaggag tacctgcggg gtccaaaagg
     3961 cttgtgaagt gcttagcggt atctttctga tttccttgag gagcaagact agaaatcgtt
     4021 gggatcaaaa acaactgatt gtgagtccct accagtcagg cagctgtatt attctttatc
     4081 ttgtttccag ttgttctggt ttggttccct ctcgctctga tatggccacg ctctctttga
     4141 aggggcgagt taatccggtg ataccgatgg ttgctcgctt agctgtgtcg tcgtactcaa
     4201 gtgtggctcg cacatcttcc tcaggcggac cgacgctttg gggttttagg acgacttcgt
     4261 gaggcggaat ggtccatttc ctcacaatgg tggctctggg gcaactgccc tcacgctgaa
     4321 ggaggtagaa ttcgtaaccg ccggattcgt gcgggatatt ccggtcaatt aggaactggg
     4381 tgtatggcag cttatgctca acagccttgc catacagctc aatggtcgat gacccctcct
     4441 tggctctccc aacgagccca ccttcgagga cccaacctcc ctttccccag aatccttcgc
     4501 catactgcgc aaccgtaatg tttcgactct cattaaagaa catattccaa cgtcctcctt
     4561 tccaagaccc agacggttcc ttggcggccc gctccgactt gtggaatatc ccgccattta
     4621 tttcaatatg caccgacgtg tgagcatctc cctcaagcat atgttgtttc cagagcgcat
     4681 cgagcggttc acgggccggc aactcgcccg cctcaaccgg ggaaattccg agtatcgaaa
     4741 atattagcga gagagtaatt atgctgatct gcattcggtt acactcactc ttcggcacac
     4801 gtcggtgtgt tcctcttccc aagcatatag gcccgtatcg tgttgatcgt gtcgcgtggt
     4861 gcttcttggg ggccaatgat gccaagaagg gctgaccgtt ttactgtacc actgtctatg
     4921 cgcgccgcat aggtgctcgc ctcgcgcagg agcatgattg gcgaacgatc ttccgccaat
     4981 ccactggatg gataaccgcg aaggacattg gatgcgggcg aaagcttttc cctactggac
     5041 tagcttcgca acgctttctt atgaagggcg caccttcaaa aacatttgtt tttggacctc
     5101 tctcgactca tcttgggtac cctgatacta cgctcgcttg caccgcg
//
LOCUS       DATIPF010000115         4920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1258018, whole genome shotgun sequence.
ACCESSION   DATIPF010000115 DATIPF010000000
VERSION     DATIPF010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258018"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VLL06_02850"
     CDS             complement(<1..392)
                     /locus_tag="VLL06_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSS29936.1"
                     /translation="MGIVNGLNLINPIDPAQLQKETQRIYEVCDGCRRCFNLCPSFNT
                     LLDGIDRYEGDVAKLTPTDHHRVVDECYYCKLCYNHCPYTPPHQYALDFPRLMIAWKK
                     HLAATRGVSWRDRLLIKTDMIGTLGSLVA"
     gene            complement(493..921)
                     /locus_tag="VLL06_02855"
     CDS             complement(493..921)
                     /locus_tag="VLL06_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HSS29937.1"
                     /translation="MGNSLKGTKSHDNLKGAFAGESQANRRYLYFARRADIEGFPDIG
                     GLFRDTSEAETGHAFGHLDFLKEVGDPATGVPIGNTEANLKSAIEGETYEYTQMYPGM
                     AKTARDEGFAELAEWFETLAKAERSHANRFTKGLDSLKQS"
     gene            complement(1042..1602)
                     /gene="rfbC"
                     /locus_tag="VLL06_02860"
     CDS             complement(1042..1602)
                     /gene="rfbC"
                     /locus_tag="VLL06_02860"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HSS29938.1"
                     /translation="MVRVTTINIPGVVLIEPDVFRDPRGLFLETYHARRYADAGIPGP
                     FVQDNHSQSLRGTLRGLHFQEPHAQGKLVMVTEGVVYDVVVDIRKGSPSFGRWYGIEL
                     SAENRHQVYVPPGCAHGFCVTSDRASFLYKCTDYYAPSAERGIIWNDPALAIPWPVAT
                     PILSAKDRLYTSLAEMESELPRYFPG"
     gene            complement(1556..2062)
                     /locus_tag="VLL06_02865"
     CDS             complement(1556..2062)
                     /locus_tag="VLL06_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29939.1"
                     /translation="MPSLDIQNPEMPDLQFVLFVSALCTADLKTLNVPWELRAAIFDR
                     CWTLIRTDAPPTDPKERVLDLREGTELTLEACLSTIRSLLTDAGIHTLSWDHPVSEPT
                     HESTPAAKPLIDRLGQLYPDPPDIVDPEKPASGLYVTPVPCISDRQADEEEHGAGHDN
                     QYPGGRAD"
     gene            2165..2437
                     /locus_tag="VLL06_02870"
     CDS             2165..2437
                     /locus_tag="VLL06_02870"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HSS29940.1"
                     /translation="MITISLMGQLQTADGERDLACDVPSPMSVRQVIQRQGIQLRHLL
                     QLIREKKVLVTINKKIASEDSLVQNGDAIRLVGHDGMGGTGLGPSL"
     gene            complement(2557..2844)
                     /locus_tag="VLL06_02875"
     CDS             complement(2557..2844)
                     /locus_tag="VLL06_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HSS29941.1"
                     /translation="MAKSMTKSQIADHLAQKTGLTKKASVQLMDDFAALAYKEAKNVF
                     VIPGIGKLVLANRKARMGRNPQTGEAIKIPAKRVVKFRVAKAAKDAILGKK"
     gene            3130..3687
                     /locus_tag="VLL06_02880"
     CDS             3130..3687
                     /locus_tag="VLL06_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29942.1"
                     /translation="MAKELPILPSTTQPADETQAEQYQYSRVFCQKENSPPLRLLLEY
                     LKSHGQTPITPPDMDDAKLDEWAWVQITLGYDRNRKPIQVFCVRDRGTYQDVFHKEKE
                     LFLGLLSAHDDIEAGLAREYVERARFVLTTRFSRDEMTDEGYDFNGWILEFYQDQCNG
                     IVQIDNQGFYSPQGDLIVDISTKEE"
     gene            3684..4307
                     /locus_tag="VLL06_02885"
     CDS             3684..4307
                     /locus_tag="VLL06_02885"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HSS29943.1"
                     /translation="MSNRGRHDSARPPLYHKTDHWFQRATAALLGKVPCRLGCTSCCI
                     GPFPITLLDVQTLQQGLAGLPPDHRHHIEQRAIEQTTQMETAFPQLTHTDLLDTWSDQ
                     EIDRLVTKFHDQPCPALEADGRCGIYDHRPLVCRSMGIPTEDCGLTHGACEVQTFIPI
                     VRLPSTFREEESALAQEEAATLDALRRATGSTGEEVFLPYGFISRKT"
     gene            4456..4532
                     /locus_tag="VLL06_02890"
     tRNA            4456..4532
                     /locus_tag="VLL06_02890"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4490..4492,aa:Arg,seq:ccg)
     gene            4589..4664
                     /locus_tag="VLL06_02895"
     tRNA            4589..4664
                     /locus_tag="VLL06_02895"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4622..4624,aa:Lys,seq:ctt)
BASE COUNT         1142 a         1431 c         1299 g         1048 t
ORIGIN      
        1 gccaccagac tcccaagcgt accgatcata tccgtcttga tcaacaaccg atcgcgccag
       61 cttaccccgc gtgtcgcggc cagatgcttt ttccatgcga tcatcaagcg ggggaaatcg
      121 agcgcatact gatgcggcgg cgtgtaagga cagtggttgt agcagagttt acagtagtag
      181 cactcatcga caacccgatg gtggtcggtg ggcgtgagct tggcgacgtc tccttcatag
      241 cggtcgatgc cgtcgagcag ggtgttgaac gacggacaca gattgaagca gcgccggcaa
      301 ccatcgcaga cttcataaat gcgctgcgtt tctttctgaa gctgtgcagg atcgatgggg
      361 ttgatcagat tcaggccatt cacgattccc attctctcct gtgatgtcct atgaatacaa
      421 gatcggaaaa cacaaagggt gagccgggaa cacaccccag ctcaccctcg tgatgtcaga
      481 cagagcccag atctaggact gcttcaggct gtcgagcccc ttcgtgaagc gattcgcatg
      541 ggaccgttcc gccttggcga gtgtctcaaa ccattccgcg agttcagcga atccctcatc
      601 ccgagccgtt ttggccatac caggatacat ctgcgtatac tcataggttt cgccttcgat
      661 cgccgacttg agattggcct ccgtattgcc gatcggcacc ccagtcgccg gatccccgac
      721 ttccttcaag aaatctagat gtccaaacgc atggcctgtt tctgcttcgg acgtatcgcg
      781 aaacaagcct ccaatatcag ggaaaccctc gatatccgcc cgtctggcaa aatagagata
      841 gcgccgattg gcctgggact cgccggcaaa tgcacccttc aaattgtcgt gactcttcgt
      901 tccttttaaa ctgttcccca taagtctcct cctccctaag ctaagaaatc ttgccgccgc
      961 cgtcagcggg atcttatcac tatgcccccg gtgaataaca tagccatcct cgtgagatca
     1021 atgggtcctt gccgcgcgtc atcagccagg aaagtaccgt ggcaactccg actccatctc
     1081 ggccaaggat gtgtagagac ggtccttggc agacaaaatg ggtgtggcaa ccggccaggg
     1141 aatggcgagc gcgggatcgt tccagatgat gccccgttcg gcgctcggcg cataatagtc
     1201 cgtgcactta tagaggaacg acgcgcggtc gctggtcaca cagaaaccat gagcacaacc
     1261 tggcggtaca tagacttggt gccgattctc cgccgaaagc tcgatcccat accaccgacc
     1321 aaagctcggt gatccctttc ggatatccac cacgacatcg tagaccactc cttctgtcac
     1381 catcacgagc ttcccctggg cgtgcggctc ttgaaagtgg agcccgcgca gcgtaccccg
     1441 cagagactgt gaatggttat cctgaacgaa ggggccggga ataccggcgt ccgcataccg
     1501 gcgtgcatga tacgtttcga gaaagagtcc ccgtggatcg cggaagacat ccggttcaat
     1561 cagcacgacc cccgggatat tgattgtcgt gacccgcacc atgctcctct tcgtccgctt
     1621 gtctgtcact tatgcacgga acgggtgtga cgtacagccc tgacgccggt ttttcgggat
     1681 cgacgatgtc aggaggatcg gggtacaatt gccctagccg atcgattaat ggcttcgcgg
     1741 ccggcgtgct ctcgtgggtc ggttcactca ccggatggtc ccagctaagg gtatgaatgc
     1801 ctgcatccgt cagcaacgag cggatcgtcg acagacaggc ctccagcgtt aattccgtgc
     1861 cttcgcgaag atcgagcaca cgctctttgg gatcagtcgg tggcgcatcc gtgcggatga
     1921 gcgtccaaca gcggtcgaaa atagcggcgc ggagttccca cgggacgttg agtgtcttga
     1981 gatcagccgt gcagagggct gatacgaaca ggacaaattg caagtccggc atctcgggat
     2041 tctggatatc caacgacggc atggcggagc attatcgatg gccacccgca cggagtcaac
     2101 gggtacacag cttcacgttt gaccgtgatc tcaatctgtg tgactcgatc aggagcgtcc
     2161 gatcatgatt accatttcct taatgggtca gcttcaaacc gccgacggcg aacgggacct
     2221 cgcctgcgac gttccctccc ccatgtccgt ccgtcaggtc atccagaggc agggcattca
     2281 gttgcgccat ctcctccaac tcattcgtga aaaaaaagtg ttggtgacca tcaataagaa
     2341 gatcgccagc gaggactcac tggttcagaa cggcgacgcg attcgactgg tggggcacga
     2401 cggcatgggc ggaaccgggc tcggtccatc gctgtagcga ggcgacaaaa gagcagggcc
     2461 ggcgcccctc gcggggagcc ggccctgtgg ttcttcatcg gcgtgtcatg aggccggaag
     2521 aaggcgtatc tcgtacgatt tacgccaaat ggtctcttac ttcttcccga ggatcgcgtc
     2581 cttggcagct ttggcgacgc ggaacttgac cacacgcttg gcgggaatct tgatggcctc
     2641 gccggtctgt ggattccgtc ccatgcgggc cttgcggttc gccaatacga gcttcccgat
     2701 cccaggaatg acgaagacgt tcttcgcttc cttataagcc aaggcggcga aatcgtccat
     2761 gagctggacg gacgctttct tggtgagccc agttttctgg gccaggtgat cggcgatctg
     2821 cgacttcgtc attgatttag ccataacgtg caacctcctt aaagaacaag atggtgaaat
     2881 gcagtgaact cgcttcgctt gactccgctc agatccaaag aacattcatg aacgaatagt
     2941 cgtgtcgctg gaagcggcca aaaccagcgg gagtctatcc aagcgctccg aggctgtcaa
     3001 ccagaaaaac acaggccagg catagggaaa cgctttcgcc agcttcttgc agtttcgaga
     3061 ccagccagcg tagagtactg gtcgcgaccg aatttatgag tcgctgttgt cgtacttctg
     3121 agtgctacta tggcaaaaga attacctatt ctgccatcga ccacccaacc ggcagacgag
     3181 acacaagcag agcagtacca gtactcgcgc gtgttttgtc agaaggaaaa ttcacctccc
     3241 cttcgtttgc tgttggaata tctgaagtca cacggacaaa ctccgattac gccgccggac
     3301 atggacgacg caaaattgga cgaatgggcc tgggtccaga tcacgctggg gtatgatcgc
     3361 aaccggaaac cgattcaagt gttctgtgtc agggatcgag gcacctatca agacgtgttc
     3421 cacaaagaga aggagctgtt cttaggcctc ctctctgctc acgacgatat cgaagcagga
     3481 ctcgcacggg agtacgtgga gcgtgcgcgg tttgtgctca ccactcggtt ttctcgggat
     3541 gagatgaccg atgaaggcta tgacttcaac ggatggatct tggagttcta ccaagaccag
     3601 tgcaacggaa tcgtccaaat cgataaccag ggcttctatt caccccaagg cgatctcatc
     3661 gtcgacattt ccaccaaaga agaatgagca accgaggcag gcacgactcc gcccgacctc
     3721 ctctgtatca caaaacagac cactggtttc agcgggccac cgccgcgcta cttggtaaag
     3781 ttccctgccg gctcgggtgt accagctgct gcatcggccc ctttccgatc accctcctcg
     3841 acgtccagac cctccaacaa ggactcgccg gacttccacc ggatcaccgc caccatatcg
     3901 aacagcgtgc gattgaacag accacgcaaa tggaaaccgc cttcccacaa ctcacccaca
     3961 ccgatcttct cgacacgtgg tccgaccagg agatcgaccg gttggtcaca aaatttcacg
     4021 atcaaccctg tccagcatta gaggcggacg gacgctgtgg catatacgat caccgccccc
     4081 tggtctgccg atcgatgggc attccgacgg aagattgcgg cctgacccac ggcgcctgcg
     4141 aagtccagac cttcatcccg atcgtgcgac tcccctctac atttcgagag gaggaatctg
     4201 ccttggcaca ggaagaagcc gctaccctgg acgcgcttcg tcgggccacc ggatcaacag
     4261 gagaagaggt gttcctgccc tacgggttta taagtcgcaa gacatagaca aacctggttg
     4321 atctggtcta tttggttcat ctagttagtt tcgttcaacc aaacaaacga gacaaaccga
     4381 acaaaccaaa tgaacaggtc cccgctggac agaggctaaa atcctgtgct agtgtcaccg
     4441 accttggttg cgggagcgcc tgtagctcag ctggatagag catcagcctc cggagctgag
     4501 ggccacaggt tcaaatcctg tcaggcgcac cagcaaacaa gcaggggaca tcagccacat
     4561 acaaatatcc gccagcgaca tacccggtgg gccgttagct cagttggtag agcagctgac
     4621 tcttaatcag cgggccgtag gttcgacccc tacacggccc accaaaaatc aaccacttac
     4681 acaacatacc aattccagac catctccgtg taggcacagt gtaggcagta aaacagagaa
     4741 aatatttttt tctgcctctc cttcccgaac ttcaaatttc ctcgtagcaa tcatgtgacg
     4801 agaaatttct ggggtgatct tctgtggaga acgcccccct cctcttatgc taaccctcgt
     4861 caagtttgat ttgagaggtc ctgtggctta actaggtacc caggcgagtc aagtatatgg
//
LOCUS       DATIPF010000116         4703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_817204, whole genome shotgun sequence.
ACCESSION   DATIPF010000116 DATIPF010000000
VERSION     DATIPF010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817204"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..312)
                     /locus_tag="VLL06_02900"
     CDS             complement(46..312)
                     /locus_tag="VLL06_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29944.1"
                     /translation="MNIQAKKRFQRWRMGIGSGTAVQLFCLCAFLMSQLSTPMLVQSD
                     QAAITSAQPLAIQGKGAESKLPAKPQKELPPIEVTTQESTSCCR"
     gene            complement(343..1626)
                     /locus_tag="VLL06_02905"
     CDS             complement(343..1626)
                     /locus_tag="VLL06_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSS29945.1"
                     /translation="MTDNDKAMTGWVGSGWSLLRTRDFGLLFSGQAVSQIGDSMNKVA
                     LLWFVYDLTGSAFKMTLIGLLQTIPPLVFGPLIGVYLDRMKKKPVMIWVDFIRAGLVM
                     LIPLLYAMEALTLERLYVLVFATSIVSTVFGPALASAVPFLVPRARLTAANALIQSTT
                     NIGLLIGPAVSGVGIAMIGAQNVLYLNTATFLVSALCLLPIRVDETRQSSARVGERAS
                     IFEDLLVGFRFVFVQHRTVLLLMLTAALYSLGASAFVFLLPVFATQLFDASPVELGWL
                     WSSLGIGMLVASVWLAWINQGDLQKRLRLISAALAVGAVAVSTLGMLQAPVMAAVLII
                     VFGGSTAVFTPVVWAMLQELTPEPLLGRVFTTFSTGAMAAAMAGMAGFGWAADAIGPA
                     ASLVGIGILLFGTATVAAVFSRRRPDSAAPDFVPV"
     gene            complement(1772..2128)
                     /locus_tag="VLL06_02910"
     CDS             complement(1772..2128)
                     /locus_tag="VLL06_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29946.1"
                     /translation="MKLLTSTGLALAIVGIGFIVAQGIRAEDRSPSGLSPSKIVMGEV
                     AQVQGEFHMAKGPEGDVTLDIVDKSYVITSQTGKEVRLELDDHTKIQNRVNPGDKVEA
                     KISSRGHTLSVTRLDR"
     gene            complement(2176..3264)
                     /locus_tag="VLL06_02915"
     CDS             complement(2176..3264)
                     /locus_tag="VLL06_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSS29947.1"
                     /translation="MRIAQVSPLWESVPPKLYGGTERIVSYLTEELVRLGHDVTLFAS
                     GDSVTSARLEAICAHALRLNTGIFNRDAPMTMLLEQALGGPGDFDIIHSHLDFLGFPL
                     ARRNTIPTVTTFHGRLDLPELQPVFREYAEMPMVSISDSQRRPVSWANWQATVYHGLP
                     RDLYNVHPNSGRYLAFLGRIAPEKRPDHAIELAKRVGIPLRIAAKVDPMDQEYFHTQI
                     EPLLSDPLIEYLGEITDTEKDEFLGQAMALVCPYDWPEPFGLVLIEALACGTPVLAYR
                     RGSIPEIIEDKVTGFICEGLEEMTEAIQHLPKIDRRQCRVTFEQRFSAERMAQDYIRV
                     YEQAIGSTYERETVATSLESWPTLSTSG"
     gene            complement(3720..4127)
                     /locus_tag="VLL06_02920"
     CDS             complement(3720..4127)
                     /locus_tag="VLL06_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29948.1"
                     /translation="MTGLILLVEADAGLGAVLSEVLRHNGYEVILVGTLMDADVESID
                     SVNAVILDHDTTAAEKELAWLDALQPCDESLPIVLMALQAPQELRRRLGEHLDIDQTN
                     ALTVVQKPFRNEELLAAVREAKGNPSPRQANGR"
     gene            4381..>4703
                     /locus_tag="VLL06_02925"
     CDS             4381..>4703
                     /locus_tag="VLL06_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29949.1"
                     /translation="MLNRWHRLSLQVKMTLIIIFIVGVSAVTTEWLEARSIRHTVEDN
                     VRGAALAVGRSVDQNVTSLAQLSNREARTKELEKILLTLPDLLNIVLYEFPAGSGKNP
                     IPIASA"
BASE COUNT         1102 a         1409 c         1220 g          972 t
ORIGIN      
        1 cgaaggcctt ttcgatttcc gaacgcagca tggaaagttg cgcggtcatc ggcagcagct
       61 tgtcgattcc tgcgtagtga cctcaatggg aggcaattct ttctgcggtt tggcaggaag
      121 cttggattcg gcccccttac cctgaatggc taacggctga gctgatgtga tcgcagcctg
      181 atcgctctgt acaagcatcg gagtcgagag ttgactcatg aggaaggcgc agaggcagaa
      241 cagctgaacg gctgtacctg acccaatacc catgcgccag cgctgaaacc gtttcttcgc
      301 ttgtatgttc atgtccatcg agatcaacac gatggagccg cttcacacag gcacgaaatc
      361 gggagctgca ctgtcggggc gtcgccggct gaacacagcc gcgacggtcg cggtgccaaa
      421 caacaaaatc ccgataccca cgaggctcgc ggctgggcca atggcatccg ccgcccagcc
      481 gaatcctgcc atcccggcca tcgcggcagc catggcacct gtactgaagg tggtaaagac
      541 acgccctaac agaggttcgg gcgtcaattc ttgtaacatc gcccagacaa ccggtgtaaa
      601 cacggcggtg ctgccgccga acacgatgat gaggaccgcc gccatcaccg gcgcctgcag
      661 cataccaaga gtactgaccg cgacagcgcc gacggccagg gccgcagaaa tcaaccgcag
      721 gcgcttctga agatctccct gattgatcca agccagccag acagaggcaa ccaacatgcc
      781 tatcccaagc gatgaccaca gccagccaag ctcgactgga ctcgcatcga atagttgcgt
      841 cgcgaataca ggaagcaaaa atacgaacgc actcgcgccc aaactgtaga gggccgcggt
      901 gagcatgaga aggagcacgg tccgatgctg gacaaagaca aaacgaaatc cgaccaatag
      961 atcttcaaag atcgatgctc tctcgcccac tcgcgcggac gattgcctcg tctcatccac
     1021 ccggattggc aggaggcaca atgcggacac cagaaacgtg gccgtattga gatacaggac
     1081 attctgtgct ccgatcatgg caatacccac cccgctgacc gctggtccga tcaagagccc
     1141 gatattggtc gtgctttgaa tcaacgcatt ggcagcggtc agtctggcgc gaggcacgag
     1201 aaacggaacg gctgaggcca acgccggacc gaacaccgtg gagacaatcg acgttgcgaa
     1261 cacgagtacg tagagtcgtt cgagcgtcag cgcctccatc gcgtagagca gggggatgag
     1321 catgaccaac cccgcacgga tgaagtccac ccagatcatc accggctttt tcttcatccg
     1381 atcgagataa acaccgatga gcgggccgaa gacgagcggc ggaattgtct ggagcagtcc
     1441 gatcaacgtc atcttgaagg cagaccccgt caggtcatac acaaaccaca gcagagcgac
     1501 cttgttcatg ctgtcgccaa tttgggacac ggcctggcct gaaaacagaa ggccgaaatc
     1561 tcgcgtcctc agcaagctcc accctgaccc tacccaacct gtcatcgcct tatcgttgtc
     1621 ggtcatctcg aaacctctcc ttttcaaccc tacttgggaa cttcaacata ccagcgacac
     1681 actgccgttg aatgtcgtgc gtctcctgcg ctcgtaactc tgcgtggtcc tgaatcggca
     1741 taatattgtt ctcctacgag agccgcgtca gtcatcggtc gagtcgggtc accgaaaggg
     1801 tgtggcctct ggatgagatc ttggcttcga ctttatcccc tggattcact ctgttttgga
     1861 ttttggtatg gtcgtcgagt tcgagacgca cctcttttcc ggtttggctt gtgatgacgt
     1921 aggacttatc cacgatgtcg agcgtgacat cgccttccgg ccccttggcc atatgaaatt
     1981 ctccttgaac ctgagccact tcgcccatca cgatcttcga cggtgacaat cccgagggag
     2041 accggtcctc cgcccgaatc ccctgcgcga caatgaagcc aataccgaca atcgccaaag
     2101 caagaccggt cgacgtgagg agcttcatat ggacctcctt ccagatgctg aagaaagcca
     2161 aaaaatgatg cacgcctacc cactggttga gagcgtaggc catgactcga gactcgtggc
     2221 cactgtctcc ctttcatagg tgctaccgat cgcctgctca tacacacgga tatagtcttg
     2281 cgccattcgt tccgcactga atcgctgctc gaaggttacg cggcattgcc gacggtcgat
     2341 cttaggaaga tgttggatag cctccgtcat ctcttcgaga ccttcgcaga tgaatcccgt
     2401 gactttgtcc tcaatgattt caggaatgga cccacgccga taggccagca cgggcgtccc
     2461 gcaggccagc gcttctatca gcacaagacc aaatggttcc ggccagtcat aaggacagac
     2521 gagcgccatg gcctgtccaa gaaattcgtc cttttcggtg tccgtgatct caccgagata
     2581 ctcgattaac ggatcggaga gcagcggttc aatttgagtg tgaaagtact cctgatccat
     2641 cggatcgacc ttcgcagcaa ttcgcaaggg aatcccaacc cgtttggcga gttcaatcgc
     2701 atgatccggc cgcttctcgg gagcaatacg tcccaggaac gccaaatacc ttccggaatt
     2761 cggatgcacg ttatagaggt ctcttggcaa accgtgatag acggttgcct gccaattcgc
     2821 ccaggaaaca ggtcgccgct gggaatccga gatcgacacc atcggcatct ccgcatattc
     2881 acgaaacacc ggctgcagtt caggaagatc gagccggcca tggaatgtgg tcaccgttgg
     2941 aatcgtattc cgccgcgcca gagggaagcc caggaaatcg agatgagagt gaataatgtc
     3001 gaagtcgccc ggtcccccca gcgcctgctc caacagcatg gtcatggggg catcgcggtt
     3061 gaagatgcct gtattcaatc gtagcgcatg agcacagatc gcttctagac gagcgctcgt
     3121 gaccgaatct ccgctcgcaa agagggtcac atcgtgaccg agccgaacta gttcctcggt
     3181 aaggtaggac acgatccgtt ccgtccctcc atagagtttc ggcggaacgc tctcccacag
     3241 aggtgaaacc tgggcgattc tcatcggact gctcctttca tcattgattc attcgccggt
     3301 cgaagtgcca cggatctctc gccaataatc ccatctgcct cgattctttc ccccgggacc
     3361 aacatgcgct ctcgctccgt attcttgtcg acctgaaggt atacaagcct cccggagaag
     3421 tccttcacga catacctatc accatctgaa gagtcatctc agactgtgcc aatgcgccgg
     3481 aaattaacgc tcggcaatcg actttgccct cccgcgtttt ccgcctccga caacagatcg
     3541 caggacaggg cgcgaatctg tttgccggaa tattgcaatc gcaccgcagt tgacacaccg
     3601 ccacccgcaa taggcccagc ccccgttggt ggtggtctca cctccgataa actgttcagc
     3661 caccaccagc ccagcacaac gttcacatct catgggtttc gcctcaaacg gcctgtgggc
     3721 tatcttccgt ttgcctgtct cggcgaagga ttccctttcg cctctcggac ggcagcgagc
     3781 aattcttcat tccgaaacgg cttctgcacc actgtcaaag cgttggtctg atcgatatcc
     3841 agatgttcgc ccaggcgacg gcgcagttcc tgtggtgctt gcaaggccat caagacgatc
     3901 gggagcgatt cgtcgcaagg ttggagtgca tcgagccagg cgagttcttt ttcagcggcg
     3961 gtcgtatcat gatccagaat cacagcgttc acggaatcga tgctctcaac gtcggcatcc
     4021 attaacgttc ccacgaggat cacctcatac ccattgtggc gtaacacttc tgacaaaacg
     4081 gcacccaatc cggcatcggc ctcgactaga agaatcagtc cagtcacgat gcatctctcc
     4141 aaaagtccaa gggtcttgcc atggactgag caaacccgat gcctagaatt aatcttgtac
     4201 cggcggtacc gccaactcgc gcaagaccgc ggaagattcg aagttttatc ttattgagcg
     4261 tcgaggagac gtgagtggta gggtgatttc tctccaagat gagagacaac ttattcctgg
     4321 gcagacatct tgtctcgaat tccgattgaa caaggaaatg tgagggacta ccatgagcct
     4381 atgctaaacc gctggcaccg gctgagcctt caagtcaaga tgaccctgat catcatcttt
     4441 atcgtcggcg tctccgcggt cacgactgaa tggctcgagg cccgctcaat acggcacacg
     4501 gtggaagaca acgtccgagg cgccgcgctg gccgtcgggc gatccgtcga ccaaaacgtc
     4561 accagccttg cccagctgtc gaatcgagaa gcgcgaacga aagagctgga gaagatcttg
     4621 ctcaccctac ccgaccttct caacatcgtg ttgtacgagt tcccagccgg atccggtaag
     4681 aatcctatac cgatcgcgag cgc
//
LOCUS       DATIPF010000117         7655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_109207, whole genome shotgun sequence.
ACCESSION   DATIPF010000117 DATIPF010000000
VERSION     DATIPF010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7655
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109207"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VLL06_02930"
     CDS             complement(<1..1062)
                     /locus_tag="VLL06_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HSS29950.1"
                     /translation="MIPEIGHYALILALCVAVVQGTVPIYGAAVRNSALMSIAKPAAR
                     GQFLLVLLAFCCLGYAFAAKDFSVLYVAATSNSQLPLHYRLAAIWGAHEGSLLLWVFI
                     LSIWMVAVSFFSTHLPEAMRARILGVMGLVSMGFLLFMLTVSNPFERLIPAAFDGRDL
                     NPLLQDPGMVIHPPMLYMGYVGFSVAFAFAIAALLGGNLDAAWARWSRPWTTVAWCFL
                     TVGVAMGSGWAYYELGWGGWWFWDPVENASFMPWLAGTALIHSLAVTDKRGSFKAWSV
                     LLAILAFSLSLLGTFLVRSGVLTSVHAFATDPKRGLFILVFLAVVIGGSLLLFAWRAP
                     RVGLGGGFSIISREGMLLGN"
     gene            complement(1071..1538)
                     /gene="ccmE"
                     /locus_tag="VLL06_02935"
     CDS             complement(1071..1538)
                     /gene="ccmE"
                     /locus_tag="VLL06_02935"
                     /inference="COORDINATES: protein motif:HMM:NF009729.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HSS29951.1"
                     /translation="MKPRHKRFIFIGLGLLALAVASVLILNAFQSNLVFFFTPTQVAN
                     GDVPKGRGFRIGGMVEDGSLKREGDGLTVHFVMTDMAKRVPVTFKGILPDLFKEGKGA
                     VAQGQLGADGIFLASEVLAKHDENYMPPEAADALAKAKAAGAQTSSTLVVNPR"
     gene            complement(1554..1712)
                     /gene="ccmD"
                     /locus_tag="VLL06_02940"
     CDS             complement(1554..1712)
                     /gene="ccmD"
                     /locus_tag="VLL06_02940"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HSS29952.1"
                     /translation="MQWGSASEFFAMGGYGLYVWVSFGATAVCMAWEVLAVWRRHTSA
                     VAESRDVS"
     gene            complement(1712..2461)
                     /gene="ccmC"
                     /locus_tag="VLL06_02945"
     CDS             complement(1712..2461)
                     /gene="ccmC"
                     /locus_tag="VLL06_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HSS29953.1"
                     /translation="MAINWYKYSSPQAFLPLAGKMIPWFSAAAVALICVGLYIGFFIA
                     PTDFQQGDSYRIIFVHVPAAWMSMFLYVVMAIWAGVGLGLNARPSFMMAQAIAPTGAM
                     FTFLALLTGAMWGKPTWGAWWVWDARLTSELILLFQYIGIILLRASIDDLRRADRESA
                     VLALVGVVNVPIIYFSVKWWNTLHQGASVTMTESKMATPMVAAMLIMMLGFWMYSFAV
                     TLTRMRCVILDRERQPSWGKHPSIQEVVEAR"
     gene            complement(2480..3163)
                     /gene="ccmB"
                     /locus_tag="VLL06_02950"
     CDS             complement(2480..3163)
                     /gene="ccmB"
                     /locus_tag="VLL06_02950"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HSS29954.1"
                     /translation="MSQPSLSEALRGVVRRDLLLAMRHRSDVAMSVFFVVIVASLFPL
                     GVGPEATVLRMIGPGVLWVTALLACLLSLGRLFTADYLDGVLEQTALIPQSLAVLVIG
                     KVFAHWVISGLPVVLLSPLLGLQFGLGGDSLGVLTLSLLLGTPTLSMIGGIGAALTLG
                     LRGSGLLVALLVLPLFVPVLIFGAGAVTSSMAGIGSEANLSLLGACFMLSLGFAPWAT
                     AVALRIALE"
     gene            complement(3160..3780)
                     /gene="ccmA"
                     /locus_tag="VLL06_02955"
     CDS             complement(3160..3780)
                     /gene="ccmA"
                     /locus_tag="VLL06_02955"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HSS29955.1"
                     /translation="MLQTVALSGSRGQRQLFSDVSVTVRPGTLLAVAGENGSGKTSFL
                     RILCGLLTPDGGMILWQGKDIHQLKELYRAQLMYIGHLNGVKDELTPIENLTLSACLA
                     GDDSSGTTVQKALEAVGLGRPVHLLPTRVLSQGQKRRVALARLWLSTRPLWFLDEPFA
                     SLDAVATTYLTQRLQSHLSNGGMVVVSTHQEIGLPPDSVQHLRLTQ"
     gene            4696..5286
                     /locus_tag="VLL06_02960"
     CDS             4696..5286
                     /locus_tag="VLL06_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29956.1"
                     /translation="MIRHVTQAGGTMLLLIAALYHPDVAAAHGSTDLEQEPCVRQLGD
                     GMVHFSAYQPQYEVKDQYCTEIPQEGDTFLVVDLVDPALRTEPVGMRVVKGNGTKETE
                     DQIIADVRPSYHPDGVLRSEVRLDEGQYTVTIQSEKRNILKRPQYLLRVHMIDYQKLV
                     LSIGGPLVGVLLFAWLVYKLIRSKWLRRWWTAAPTS"
     gene            5348..5932
                     /locus_tag="VLL06_02965"
     CDS             5348..5932
                     /locus_tag="VLL06_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29957.1"
                     /translation="MFVQFLKPVGLCILMLAIGAPFSTEAHAHGGLALAEDMCRLTIG
                     PYNMHFTGYQPDNTRNKEFCEDIPATGRTVVVLDYMDDALRPLPTEVRVIRDTGSEQD
                     IQAITVLHLPPKVYPNGTVNFEYNFAQPGKYVGLVTVGGPKEHISRFPFSVGEGKMWV
                     PPSYLLMAFAGAAVVVVLIFALRGRRNTSNSTAS"
     gene            6149..6739
                     /locus_tag="VLL06_02970"
     CDS             6149..6739
                     /locus_tag="VLL06_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29958.1"
                     /translation="MSLPFLTSAGLCFVMLVLGVPLSAQAQHTHELEKNFCILKIGPY
                     GMHLTGYQPDLAGGKENCGEVPGTGRTVLVLDYIEGELRSMPVEVRVIKDTGSEQDLQ
                     AITVLHVPPKIYPSGFISLDHNFDQPGKYVGLITLGGTQEHHMARFPISIGESNVVSH
                     FMHYIMVIAPLAAIAGGAAIFFFLRGRRKTSDNTVS"
     gene            6846..7250
                     /locus_tag="VLL06_02975"
     CDS             6846..7250
                     /locus_tag="VLL06_02975"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HSS29959.1"
                     /translation="MESWAVQAFKRLVIGTVMATAMCAHVTPALAHAALVKTEPPRRA
                     IVAKAPAQVQLWFNEEIESAYASLVVLDTGKNPVTEAKPQLASNDLKSIVLPLPELAP
                     GKYSVKFRVLSLDGHVIQSSFDFTVKDKLQAK"
     gene            7247..>7655
                     /locus_tag="VLL06_02980"
     CDS             7247..>7655
                     /locus_tag="VLL06_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29960.1"
                     /translation="MIEVIAALIRWLVLASNMILIGGCVFLAIAGRERAAFDNPWFAC
                     LKRTLPWLAVMLLLSLLGLLATTTAQVTGVGENAWHPGAWLDLLQKTRIGLIWMGRAA
                     LALLLFGVALFVRFSPPARWQYVLCATVAALALA"
BASE COUNT         1865 a         2225 c         2055 g         1510 t
ORIGIN      
        1 gttccccagc aacatcccct cgcgcgagat gatggagaaa ccaccgccta gtcccacacg
       61 tggcgcacgc cacgcgaaca acagcagtga gcctccgatt accacggcca ggaagaccag
      121 gatgaataac ccacgctttg gatcagtggc aaacgcatgc acggaggtca gcaccccgga
      181 gcggactaag aaagttccga gcaagctgag tgaaaaagcc aagatggcga gtaggacact
      241 ccaggctttg aaactgcccc gtttatctgt gaccgccagc gagtgaatca acgccgtccc
      301 agccaaccag ggcatgaacg aggcgttttc aaccggatcc cagaaccacc aaccacccca
      361 gcccagctcg taataggccc agccgctccc catcgcgaca ccgacggtca gaaagcacca
      421 ggctactgtg gtccagggac gtgaccagcg cgcccaggcc gcatccaggt ttccccccaa
      481 gagcgcggcg atcgcaaagg caaaggcgac ggagaatccc acgtacccca tgtaaagcat
      541 tgggggatgt atgaccattc cggggtcctg cagtaaggga ttcaaatccc ggccatcgaa
      601 cgcggctggg attaagcgct cgaacggatt ggagaccgtt agcatgaaca agagaaatcc
      661 catgctgacc aaccccatca caccgagaat ccgcgcacgc attgcctcgg gcagatgggt
      721 agagaagaac gacacggcga ccatccagat gctgagaatg aatacccaga ggagcagcga
      781 tccttcatgt gctccccaga tagccgcgag tcggtaatga agggggagct gggaattcga
      841 cgtggccgcg acgtagagaa cggaaaaatc tttcgccgca aaggcatacc ctaaacaaca
      901 aaaggccagc aggaccagga gaaattgccc gcgcgcggcg ggcttcgcga tagacatgag
      961 ggcagagttc ctgaccgcag caccatagat gggaacggtc ccctgcacca ccgccacgca
     1021 gagcgcgagt atcaaagcat aatgaccgat ttctggaatc atcttggctc ctagcgtgga
     1081 ttgacgacga gcgtgctgct ggtttgcgct ccagctgcct tggctttggc cagcgcgtca
     1141 gccgcttccg gcggcatata gttttcatcg tgctttgcca gcacctcgct cgcaaggaat
     1201 atcccgtcag ccccaagttg cccctgggcg accgcgccct tcccttcctt gaataagtca
     1261 ggaaggatcc ccttgaacgt cacaggcaca cgcttcgcca tgtcggtcat gacgaaatgt
     1321 acggtcagcc catcaccttc acgtttgagg ctcccatctt cgaccatccc gccgatacgg
     1381 aatccgcgcc ctttcggcac atcgccgttc gccacctgcg tgggcgtaaa aaagaagacg
     1441 agattgcttt ggaatgcatt cagtatcagc accgaagcca ccgccaacgc aagcagaccg
     1501 agaccgataa agataaagcg tttgtgccga ggtttcatcg aatcagtttc ctcctaagac
     1561 acgtcacgac tctctgccac cgccgaggta tgtcgacgcc agactgccag gacttcccag
     1621 gccatgcata cggccgtggc accgaatgag acccacacat agagaccgta cccgcccatc
     1681 gcgaaaaact cactcgcact cccccattgc atcagcgggc ctccaccact tcttgaatcg
     1741 agggatgctt gccccatgac ggttgtcgct cgcggtccag gatcacgcaa cgcatgcggg
     1801 tgagtgtgac agcaaaactg tacatccaga accccaacat catgatcagc atcgccgcta
     1861 ccatcggcgt cgccattttc gattccgtca tggtgaccga cgccccctgg tgcaacgtgt
     1921 tccaccactt cacggaaaaa tagataatcg ggacattgac cacaccgacc agcgccaaga
     1981 cggcactctc ccgatcggct cgacggagat catcaatcga ggcgcgtaat aagatgatcc
     2041 ctatgtactg aaacaacagg atcagctccg acgtcagtcg agcatcccac acccaccacg
     2101 caccccaggt cggtttaccc cacatcgcac cggtgagcag cgccagaaac gtaaacattg
     2161 caccggtggg agcgatggcc tgcgccatca tgaaggaggg ccgtgcattc agccccaacc
     2221 caacgccggc ccaaatagcc atcactacat acaaaaacat ggacatccag gcggccggca
     2281 catggacaaa gataatgcgg taggagtcgc cttgttgaaa gtcggtcggc gcaatgaaaa
     2341 atccgatata gaggccgacg cagatcaacg caacggcggc agcggaaaac catgggatca
     2401 tcttcccggc cagcgggagg aatgcttgcg gcgacgaata cttataccag ttaatagcca
     2461 taacgcttat gagaacgcat tattccaatg caatccgcaa tgcgaccgcc gtggcccatg
     2521 gcgcgaatcc gagcgagagc atgaaacaag ccccgagtaa ggacaggttt gcttcgctcc
     2581 cgattccggc catactactg gtgaccgcgc cggccccaaa tatcaacacc ggaacaaaga
     2641 ggggaagtac caggagggca accagcaaac cactcccgcg taatccaagg gtcagtgcgg
     2701 caccgatccc accaatcata cttaacgttg gcgtgccaag caagagcgat aacgttaata
     2761 cccccaagga gtccccaccc aggccaaatt gcaggccgag aagaggcgac agcaggacca
     2821 ccggcaagcc agaaatcacc cagtgagcga atacctttcc aatgaccaac accgccaacg
     2881 actgcgggat caaggccgtc tgctccagga caccatcaag ataatcggcc gtaaataggc
     2941 gcccaagcga caacaggcac gccagcaggg ccgtgaccca gagcactccc ggaccgatca
     3001 tcctcaagac cgtcgcctca ggtccaacac ctaatgggaa aaggctcgcg acgatgacga
     3061 cgaagaaaac agacatcgcc acatccgagc ggtgccgcat ggccaacaac agatcccgcc
     3121 ggacaacccc acgcaacgcc tcactcaggc tgggctgact cattgagtca gcctcaggtg
     3181 ctggacggaa tcagggggga gcccaatctc ctgatgcgtg gaaacgacga ccatgccgcc
     3241 attgctcaag tgtgactgaa ggcgttgggt caagtaggtt gttgccacag catcgagtga
     3301 cgcgaagggc tcatccaaga accataacgg acgggtggaa agccacagac gcgccagtgc
     3361 cacgcggcgc ttttgtccct gtgaaaggac ccttgttggc agaaggtgaa ccggccggcc
     3421 gagaccgacg gcctccaacg ccttttgcac agttgtgccg gaactgtcat cgccggctag
     3481 gcaagcggag agcgtgaggt tttcgatcgg agtcagctcg tctttgaccc cattgagatg
     3541 cccaatatac atgagttggg cacgatagag ctccttgagt tgatggatgt ctttcccctg
     3601 ccaaaggatc attcctccat caggcgtcaa taggccgcac agaatgcgga ggaagctggt
     3661 tttcccacta ccgttttcac ctgcgacagc caacagagtt cccggtctaa ccgtcacgct
     3721 gacgtccgag aacaactgtc gttgcccacg actgccactc aatgcgacgg tttggagcat
     3781 attgggagaa gtctcacagg gaccacaggg accgaggaaa tagaattgca ggagtgtcga
     3841 acccctttgg gtctcgctgt ccgtgagcgt gtgtagacat cgtttgatac cgtaatacga
     3901 tacaactgtt gctgaagcag tagtcccttc gcataaccga tgtcacgaca aatgatggta
     3961 atttccggct tcttggaagg aagcacacgt cacatttggg attgtctccc atctggcatc
     4021 ggcaagtcaa agtgggttgg gttctgtacg gtataaacat ctccccgagg tctatgattc
     4081 ccatctaagt tcgagtcctt ttgtcaccag acactctttg aatgtccttg cctctcaggt
     4141 atcgcaccaa aaggtaaatc aggtcagttc cccccaagta cccggtcctg ccatgccggc
     4201 gaccatagcc ggtactcacg agcacacggc aacacttctc tcgacacaca ggtgcaattt
     4261 tcataagcct attacctagg gcgcaatggg ctggagcaat agattccatt tctgcttttt
     4321 gttgcaacag tccctaccag cctcatgtat ggaaaagccg accgcggttc ttacaccggc
     4381 tgctcattat ggtagagctt cacaatctgg acatgcatgc ctgtccttgt atcagcgtgc
     4441 tgctggtcag gcacgattca tttgcgaaag cagggaccca ggccatgccg agttcagcca
     4501 acagcgcatt attgtttttt ttccacttgc cttgtgcgaa agggaggggg ctagaatctt
     4561 gcctcgtttt agtggcatca tgatgggcat ctatgatatg aaatgtcgct tttgacgcca
     4621 ttacaaattg gggttgttat gccgcaccta gcaagggatc agcacaagcc ccaaagagga
     4681 gggtccggca taaatatgat acggcatgtg acacaggcag gcggaaccat gctcctgctt
     4741 attgcagcac tgtaccaccc cgacgtagcc gcggcacatg gaagcacgga tttggagcaa
     4801 gagccgtgtg tgcgccaact cggtgacggc atggtgcatt tcagcgccta tcagccgcaa
     4861 tacgaggtga aggatcaata ctgcacggaa attccccagg aaggcgacac gttcttagtt
     4921 gtggatctcg tggaccccgc gttgcgtacc gagccggtcg gaatgcgggt cgtgaaggga
     4981 aatggcacca aagagacaga ggatcaaatt atagcggatg tgcgtccgag ctaccatccg
     5041 gacggagtgc tcaggagcga agtcagactg gatgagggcc agtatacggt taccatacag
     5101 tcggagaaac ggaacattct gaagcgcccg cagtatctcc tgagggttca catgatcgac
     5161 tatcagaagc ttgtgctttc cataggaggc ccgctagtag gcgtcctact atttgcctgg
     5221 cttgtgtaca aactcatacg atcgaagtgg ctgcggagat ggtggactgc cgcccccact
     5281 tcttagcaaa ccaggcgttc cgatcagcga tcatatgatt caccattcca gagggaggac
     5341 ccggacaatg tttgtgcaat tcctgaaacc ggttggcctg tgcatcctga tgctcgcgat
     5401 cggggctcca ttctccactg aggcccacgc gcacggagga ctggcattgg cagaggacat
     5461 gtgcagactc acgatcggcc cttacaacat gcatttcacc ggttatcagc ctgataacac
     5521 acgtaataag gaattctgtg aagacattcc ggcaaccggc cgcacggtcg tggtgctcga
     5581 ttatatggat gatgcgctcc gcccgttgcc taccgaggtt cgggtcatca gagacacggg
     5641 ctcggagcag gatattcagg cgattacggt cctccaccta ccgccaaagg tctatcctaa
     5701 cggcacggtc aattttgaat acaacttcgc acagcccggc aaatatgtcg ggctggtaac
     5761 cgtaggtggc ccgaaggagc acatatctcg gttccccttt tcggtgggag aagggaaaat
     5821 gtgggtaccc ccctcctact tactaatggc gttcgcagga gctgccgttg tcgtcgtgct
     5881 catttttgcc ctgcgcggtc gtcgtaacac ttcgaatagc accgcgtcgt aagcggcatg
     5941 agagtcatga gccgaaataa gacggctaaa cggctaccgg tgcgtatggg aaccgcagcc
     6001 aaggatgaag aagaactccg atggaatcag aggccatatg cacgcatcgt gctggaagac
     6061 gtgcgtgcat gtcacctcgg cgttagggca gctctagttc aacgagaaaa ttgagcgccc
     6121 ctaaactcct acagtaagga tactgacaat gtctttgcca tttctgacat ctgccgggct
     6181 gtgcttcgtg atgctggtgc tcggagtgcc cctctccgca caggcccaac acacgcacga
     6241 attggagaag aacttctgca tactcaagat cggaccctat ggcatgcacc tgaccggcta
     6301 tcagcctgac ctcgctggcg ggaaggaaaa ctgtggggaa gttcctggga cgggccgcac
     6361 cgtactggta cttgattata tcgaaggaga actccgctct atgcccgtcg aggttcgggt
     6421 gatcaaggac acgggctcgg agcaggatct tcaggcgatc acggtccttc acgtgccgcc
     6481 caagatctat cccagcggct tcatcagtct cgaccacaac tttgaccagc ccggcaaata
     6541 tgtcgggctg ataaccttag gcggtacaca agagcatcat atggcccgat tccccatctc
     6601 gataggagaa tcgaatgtcg tttcgcattt catgcactac atcatggtca tagccccact
     6661 cgcagcaatt gccggtggag cggcgatctt cttcttcctg cggggtcgtc gcaaaacttc
     6721 tgacaatacc gtatcataag atgtctggcc atcctgtttt ttagagaacg atgcgccgga
     6781 caaaggcagc cgaacagccg tcagctgtct tcaagctcgg ctatcacaga ctagaaggag
     6841 ggccgatgga atcatgggca gttcaggcgt tcaagcgtct cgtgataggc acagtgatgg
     6901 cgacggcaat gtgtgcacat gtcaccccgg cgctggcgca tgcagcgctg gtcaagacag
     6961 agccgccgcg ccgggcaatc gtcgccaaag ctccggctca ggtacaactc tggttcaacg
     7021 aggaaattga gagtgcgtat gcgtcgcttg tcgtactcga cactggcaag aatcctgtta
     7081 cagaggccaa acctcaactt gcttccaacg acctaaaatc cattgtcctg ccgctacccg
     7141 agttggcgcc tggaaagtac tctgtgaagt tccgggtcct atccttggac ggtcacgtga
     7201 ttcagtcatc cttcgacttc accgtaaagg acaaactaca agcgaaatga tcgaggtcat
     7261 cgcggcgcta atccgctggc tggtgcttgc atccaacatg atcctgatcg gcggttgcgt
     7321 ttttcttgca attgccgggc gcgagagagc cgcattcgac aatccatggt tcgcctgcct
     7381 gaagcggacg ttaccatggc tggcagtcat gcttctgctg agcttgctag gcttactcgc
     7441 tacgaccacg gcgcaggtta caggcgttgg cgaaaatgcg tggcatccgg gagcttggct
     7501 agatctctta cagaaaaccc gcataggact catctggatg gggcgcgcag cccttgcgct
     7561 tctcttgttc ggagtggccc ttttcgtgcg gttctcacct ccggcacgct ggcaatatgt
     7621 gttgtgcgca accgtcgctg cgctcgcgct ggctg
//
LOCUS       DATIPF010000118         1863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_22099, whole genome shotgun sequence.
ACCESSION   DATIPF010000118 DATIPF010000000
VERSION     DATIPF010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22099"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VLL06_02985"
     CDS             <1..769
                     /locus_tag="VLL06_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HSS29961.1"
                     /translation="RDRSGTICGLLALMADVSRRKQVESQLNQSSGQLRALAKRLESV
                     REEESARIAREVHDELGQAMTSLKLDLSWVVRRLSIPETADTRRQLLERIHGTMQQLE
                     LTIQTVRAIATALRPPVLDELGLAAALDWQTHDFEKRTGIRCEWSMPLVPIPIGPDQA
                     TAIFRIYQEILTNVVRHAQASTICVHLGISNGWLVLEACDNGRGISDATLSNRNSLGL
                     LGMRERAAQWGGDVSILGAEGMGTTVKVRLPLLRGIA"
     gene            766..1401
                     /locus_tag="VLL06_02990"
     CDS             766..1401
                     /locus_tag="VLL06_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSS29962.1"
                     /translation="MIRILIADDHPFVRRGVKQVLTDEFPGVVIGEASTAPEVLELAG
                     KHPWNLVVLDLTMPGRDGLDALHELKSRCPDTPVLVLSMHPEDQFAVRVLRAGAAGYL
                     TKESIPEELVQAVRKIMTDGKYIRPSVADLLATQLQQGDANQPPHARLSDREFQVLGL
                     IAHGKSVTHIAEELSLSVKSVSTYRARIVEKLHLKSTADLTRYALEHRLIV"
     gene            1577..>1863
                     /locus_tag="VLL06_02995"
     CDS             1577..>1863
                     /locus_tag="VLL06_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29963.1"
                     /translation="MPPHCTQKAPHAGNRWLLPLLPSILAAVSPIATVDPIRLAAATD
                     SPITEDGCTIQRMVTGTGTTSSVLPLTSTPEQNTYEGFAPSSVLEIAPNMTR"
BASE COUNT          408 a          533 c          543 g          379 t
ORIGIN      
        1 ccgagatcga agcggcacca tatgcgggtt gcttgccctt atggccgacg tgagtcggcg
       61 caaacaggtg gagtcccaac tcaaccaatc ctctggtcag ctacgggccc tagcgaaacg
      121 attggaatcg gtgcgggaag aggaaagtgc gcggatcgcc cgcgaggtcc acgatgagtt
      181 ggggcaggcc atgaccagct tgaagctgga tttgtcctgg gtcgtaagac ggctctctat
      241 tccagagacg gctgatactc ggaggcaact gctggagcgg atccacggga cgatgcagca
      301 gcttgaactc acgatccaga cggtgcgtgc gatcgcgacc gcgttgcggc cccctgtatt
      361 agacgagctc ggcctcgccg ccgcgcttga ctggcagaca catgattttg agaaacgaac
      421 cgggatacga tgtgaatggt cgatgccgct ggtccctatt ccgatcgggc cggatcaggc
      481 cacggcaatc tttcgtatct atcaggagat cctgaccaac gtggtgcgtc atgcccaggc
      541 ctccaccatc tgtgtccatt tgggtatctc aaatggctgg ctcgtcctcg aagcgtgcga
      601 taatgggcga ggtatttcgg acgctaccct gtccaaccga aattccttgg ggctgcttgg
      661 aatgcgagaa cgggctgcgc aatggggtgg cgacgtgtcg atcctggggg ctgagggaat
      721 gggaacaacc gtgaaggttc gattgccgct cttgagagga atcgcatgat acggattctc
      781 atcgccgacg atcacccatt cgtgcgccga ggggtcaagc aagtgctgac cgatgaattt
      841 cctggagtgg tgatcgggga ggcgtccacc gccccggaag tactcgagct ggcaggaaaa
      901 catccgtgga atttggtcgt actggacctg acgatgcccg gtagggacgg gctggatgca
      961 ttgcatgagt tgaagtcacg ctgccccgac acaccagtgc tcgtactgag catgcatccg
     1021 gaggaccagt tcgccgtacg ggttcttcga gctggagccg ccgggtacct gaccaaggag
     1081 agtatcccgg aggaactggt acaggccgtc aggaagatca tgacggacgg aaaatacatt
     1141 cgcccctctg tcgccgacct ccttgccacg caactccagc agggtgatgc caatcagccg
     1201 cctcacgcca gactgtcaga tcgtgagttc caggtcttgg gcttaattgc tcatggcaaa
     1261 agcgtcacgc acatcgccga agaactctct ctgagcgtga aaagtgtcag tacctatcga
     1321 gcacgcatcg tggagaaact ccatctcaag tcgaccgcag atctgacgcg gtatgcgctc
     1381 gaacatcgtt tgatcgtcta acctcgttcc tgtttctctc ccattctgtc cttcccgagg
     1441 cctccagaca cacgtaggga acatcccaca tcaatacggc agaacctctg gcatcggaat
     1501 tgggcggtgg cctatcgcac cccatactca tgtcgtttgt aatgccgagt aggcgcggaa
     1561 aagagaggag aactctatgc cgcctcactg cactcagaaa gcaccgcatg caggcaacag
     1621 gtggctgttg cctctgctac cgtcgatctt ggccgccgtg agccccatcg caaccgttga
     1681 ccctatcaga ctggctgcgg caactgactc tccaattacc gaagatggct gcacgatcca
     1741 acgtatggtg acagggacgg gcaccacgtc aagcgttctt ccactcacca gcaccccgga
     1801 gcaaaatacg tacgagggat ttgctccgtc ttcagtcctg gagatcgcgc caaatatgac
     1861 tcg
//
LOCUS       DATIPF010000119         9012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1258967, whole genome shotgun sequence.
ACCESSION   DATIPF010000119 DATIPF010000000
VERSION     DATIPF010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258967"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VLL06_03000"
     CDS             <1..281
                     /locus_tag="VLL06_03000"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HSS29964.1"
                     /translation="DDGTHTPYRPGSRGPIPSSSPRSPTRLRARDPAPRGFTSRADPT
                     STSLLLPFELYEVIEQVSVLRIGVLLLNLAIVAYLVSQLRRHTLRSRT"
     gene            380..760
                     /locus_tag="VLL06_03005"
     CDS             380..760
                     /locus_tag="VLL06_03005"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS29965.1"
                     /translation="MAPPHYSRTYHRFPLHYPVVFGGAPFVGEGILTNLSLKGCSVLC
                     DREVLRGSDVRVSVLLPDQRTALSIELGRIKWVQGEQFGVEFLRVPLEARQRLNSMLR
                     VELIGLLKARAARNARPELINRNN"
     gene            complement(860..1963)
                     /locus_tag="VLL06_03010"
     CDS             complement(860..1963)
                     /locus_tag="VLL06_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HSS29966.1"
                     /translation="MEHPAGEPIVSIQVEHVTKRFGTFTVLNDVSLHVQTGELIALLG
                     PSGCGKTTLLRILAGLERPDEGKILLHGNEVTDRQITDRRVGFVFQHYALFRHMTVTE
                     NVSFGLRVLPHAQRPSPDQIQKKVNDLLALVQLQAMADRYPNQLSGGQRQRVALARAL
                     AVEPKMLLLDEPFGALDTKVRKELRRWLRRLHDELHITSILVTHDQEEALEAADRVVV
                     MHQGHIEQTGTPEEVYEHPATPFVYRFLGDVNVFHGRISQDETPREETAKELPPGADS
                     SGEHEVTFVRPHDLELSPTRTTTAQLEATVRFVIAAGSRVRLELVSKDSPQTIEVELT
                     KERYRELQLRQGDVVFVHPKQQQIFRMPGPSSV"
     gene            complement(1933..2841)
                     /gene="cysW"
                     /locus_tag="VLL06_03015"
     CDS             complement(1933..2841)
                     /gene="cysW"
                     /locus_tag="VLL06_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HSS29967.1"
                     /translation="MSIEIAAKVRNEEWKASLTGESTWVRWTLIALALGYLTLFLFIP
                     LAAVFAEALKNGLEGYFAPFQDPDTLAAIRLTFIAASLAVPLNLVFGVAAAWAIAKFE
                     FVGKQLLITIIDIPLAVSPVISGLIYVLLFGAQGWVGPWLIEHDIRIIFALPGIVLAT
                     IFVTVPFIARELIPLMQSQGREEEEAAFTLGASGWQTFIRVTLPNIKWSLLYGVILCN
                     ARAMGEFGAVSVVSGHIRGLTNTLPLHVEILYNEYNAVGAFAVASLLTLLAIVTLIVK
                     TVIERKSAPSRSTHDPAWNTQPGSPS"
     gene            complement(2854..3690)
                     /gene="cysT"
                     /locus_tag="VLL06_03020"
     CDS             complement(2854..3690)
                     /gene="cysT"
                     /locus_tag="VLL06_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HSS29968.1"
                     /translation="MTLVLKQPSVLPGFGLTLGFALFYFSLIVLIPLSGLFVKTSSLT
                     WEQFWHTVTEPRALASYRLTFGASFLGALINGFFGSVVAWVLVRYRFPGRSIVDALVD
                     LPFALPTAVAGITLTAIYSSNGWIGQYLEPLGLKIAFTPLGVLVALTFIGLPFVVRTV
                     QPVLQDLDKEVEEAATTLGANRWQTVRAVIMPELWPALLTGMAMAFARSVGEYGSVIF
                     IAGNMPLKSEITPLLIMTKLEQYDYAGATALGVVMLVASFLLVLMINLLQWWSSARHT
                     AR"
     gene            complement(3705..4727)
                     /locus_tag="VLL06_03025"
     CDS             complement(3705..4727)
                     /locus_tag="VLL06_03025"
                     /inference="COORDINATES: protein motif:HMM:NF008022.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HSS29969.1"
                     /translation="MMFKPFVLTLALLTGAVGFASSIQAGQSISLLNVSYDPTRELYQ
                     EFNAAFAKHWLAKTGQSVSIKQSHGGSSKQARAVIDGLEADVVTLALAYDIDAISEKA
                     GLLPANWQSRFPHNSAPYSSTIVFLVRKGNPKGIKDWDDLVKPGISVITPNPKTSGVA
                     RWNYLAAWGYALKQPGGDDTKAQDFVQRLFKNVPVLDSGARGATTTFVERGIGDVLIN
                     WENEILLSTKGSGKDKFDIVVPPVSILAEPTVSLVDKVVDKRGSRQVAQAYLEYLYSE
                     EGQDIAGKNYYRPHLQSVAAKYATTFPTVERFTLQEFFGNWQKAHKTHFADDGTFDQI
                     YLKGPK"
     gene            complement(4758..5492)
                     /locus_tag="VLL06_03030"
     CDS             complement(4758..5492)
                     /locus_tag="VLL06_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29970.1"
                     /translation="MNQDQNNWAQPSAWPLRAPLIIVGIFLTIGCAGQPVTSVRTILE
                     AGLNTVRVEEDPDSSSNAHPVTLTPAEVGTLLRGVRAVERRNIVHRLLFGQADPTRAF
                     RKEEIAVLAGPLSTALSLAEPTERVYFNLSHATDQGDQETTTGCISIRGSILYLTISG
                     VHLRHGPGPDIGKYERQLPNIPETSASYDVLFEPEEFLAKASSSARFWAPDQREELQI
                     RYQDALAALTVQPSPERDGKKLPTKP"
     gene            complement(5492..6313)
                     /gene="modA"
                     /locus_tag="VLL06_03035"
     CDS             complement(5492..6313)
                     /gene="modA"
                     /locus_tag="VLL06_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HSS29971.1"
                     /translation="MTGRTLVLRSLLLFAALTTCILTAPSVQAAPETLTIAAANSLRD
                     AFRQLLPLFEAQNKDINVRVIYGPSKTLIKQIEEGAPVDVFLPSQVEDIDQLEKKGLV
                     IQGTKRVYAGTSLVLIAGPEFPAPITSIQDLRTTLVRYIAVGDPKTSAVGKVAAQYFN
                     YSKLDSQLKSQFVYGEHSRAVLDLVAKGEADLGVVYRTDAVSNKKIRILDEAPAGSHQ
                     PVHYGVAAVWTAKNIPAAGDFINFLETPKAQALLQDHGFDRIAPQVGVAQRQEGQ"
     gene            complement(6355..7992)
                     /locus_tag="VLL06_03040"
     CDS             complement(6355..7992)
                     /locus_tag="VLL06_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29972.1"
                     /translation="MRIFGALFLLMMTITFTRVPSVQAVEEGQLVKKDGKWEYYAGED
                     PGLKYLYLKGIINEDEYNKGLKVLETKERIAKPNFNIDVNNGLNIRVGEKFLLKVRLL
                     AQARYSYSTFNDAWGTVGNSRNAEILGGQVEFRAFRRQSDSSQFTVPRVRLQFLGHAF
                     DPDLRYNVSVAMDSTPWNQEGGNGRAALQDAYISSWHIPWATVQVGQQTVWFNRALIS
                     SIATSTFADNMIVQNMFASNLQNSRDVGISILSNEDQYRFNYAFGLWGGVGPNLARDG
                     TAVSQNVLPPSNGAGTLPSGTTRTYNYDTRFMSGEIMYTARLLYKIAGNPGYGQGDIL
                     NSRTPQAAIAFGYAYDPAQNYLSAIRSDIVDRSYRQAVTAAQNGRLLGGGIYDFQTYE
                     TDFIVKYQGWSLQAEGYYRHQRVRNSDSGTSPFDLSTIPTSPLAVGPPVDLGQAWGWY
                     VQVGKYVIPRKLELAVRYGVLDPSTKQQNDLTKEFGASINYSFDGTYNNRLVVDYSNF
                     TVGSGGRAPDRFPFENQPGFGSDLIENRVNVQYQFYF"
     gene            complement(8133..8324)
                     /locus_tag="VLL06_03045"
     CDS             complement(8133..8324)
                     /locus_tag="VLL06_03045"
                     /inference="COORDINATES: protein motif:HMM:NF021566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HSS29973.1"
                     /translation="MPHINGLLDETDHHLANTILRAIREIRYGSVEIIIHDSRIVQIE
                     RKEKIRLDTDPAHTTQRAR"
     gene            8659..8883
                     /locus_tag="VLL06_03050"
     CDS             8659..8883
                     /locus_tag="VLL06_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29974.1"
                     /translation="MSRLRDSTGQSGNDIGHDVELRVQWEVNSNVDFDAGYEHWFKGS
                     YFDRLPAGTGLPAGGNKDTDFFYLQMRVRL"
BASE COUNT         1905 a         2420 c         2590 g         2097 t
ORIGIN      
        1 ttgatgacgg cacgcacaca ccataccggc ctggctcccg cgggccgatc cccagctctt
       61 cgcctcgtag tccgacccga ctccgagcgc gcgatccggc cccgcggggt ttcacctcgc
      121 gggccgaccc gacgtcgacg agcctgttgc taccgttcga actttatgag gtcattgaac
      181 aggtgtcagt cttgcgaatc ggtgtactgc tgctgaatct ggccattgtg gcgtatctcg
      241 tcagccaatt gaggcggcat acattacgat cacggacgta gattccaacg cctcaagcga
      301 acgcatgatg ttccccctcc taaggtagga taccgcttag agcgccccct ctgtacaatc
      361 cggcctcacg gaggttgtga tggcgccacc ccattattct cggacctatc atcggttccc
      421 actccactat cctgtcgtct tcggcggggc tccatttgtc ggcgagggta tcctgaccaa
      481 cctgtcgctg aagggctgtt ccgtcctttg cgatcgtgag gtcctccgcg gtagcgatgt
      541 gcgcgtgagt gtcctgctcc cggatcaaag gacggcgttg tccattgaac tcggcaggat
      601 caaatgggtg cagggtgagc agttcggagt ggaatttctt cgcgtgcccc ttgaagcacg
      661 gcagcgtctc aatagcatgc tgcgagtgga gctgatcggt ctgttaaagg cccgcgcggc
      721 cagaaacgcc aggccggaat tgatcaatcg gaacaactag gcgtgagtcc tgtactcata
      781 cagagcgccg tcacccgcca gttgccaccg cgtttcaatc atgaagagcc gatggcatat
      841 gtgctccatt ggcactccgt tacacagatg aggggccggg cattctgaat atctgttgct
      901 gtttcgggtg cacaaagacc acatcgccct gtcgaagctg taactcgcga tagcgctctt
      961 tggtgagttc gacttcaatg gtctgcggcg aatccttcga taccagctcg agccggactc
     1021 gcgatccggc ggcgatgaca aatcgcacgg tcgcttccag ttgtgccgtc gtggttctgg
     1081 ttgggctgag ttccagatcg tgcgggcgga caaaggtgac ttcgtgttcg ccagaagagt
     1141 ctgcccctgg gggaagttcc ttcgcggtct cctcgcgcgg agtctcgtcc tgagaaatcc
     1201 ggccatgaaa cacgttgaca tcgccgagga agcgatacac gaagggggtg gccgggtgtt
     1261 cgtacacttc ctccggcgtg ccggtctgct cgatatgtcc ctggtgcatg actacgacac
     1321 ggtctgccgc ttctaaggct tcttcctggt catgtgtcac aaggatactg gtaatgtgca
     1381 attcatcatg gagccgccgg agccagcgtc tcagttcttt tctgactttg gtgtcgagtg
     1441 cgccaaaggg ctcatccagc aggagcatct tcggctccac ggccagcgct ctggccaggg
     1501 ctacgcgttg acgttgcccg ccggaaagct gatttgggta gcggtcggcc atcgcctgca
     1561 actgtaccaa tgcgagcaag tcgttgacct tcttttggat ctggtcaggg gagggccgct
     1621 gtgcatgggg aagcactctc agcccgaagg agacgttttc tgtgacggtc atatgccgaa
     1681 acaacgcgta atgctggaag acgaatccca cgcgacgatc ggtgatttgc cggtctgtga
     1741 cctcgttgcc atgcaggaga attttcccct cgtcgggcct ttccaatcct gccagaattc
     1801 tgagcagggt ggtcttgcca caaccggagg ggccgaggag cgcaataagt tctcctgtct
     1861 gcacatgaag gctcacgtca tttagaacgg tgaacgtgcc gaatcgtttg gtgacgtgct
     1921 cgacctgaat actcacgatg ggctccccgg ctgggtgttc catgcaggat catgcgtcga
     1981 acgtgatgga gcggatttcc gttcgatgac cgttttcacg ataagcgtca caatagccaa
     2041 caaggtgagg agcgaggcca ccgcgaaggc cccgacggcg ttatattcgt tgtacaggat
     2101 ctcgacatgc agcggcaatg tattggtgag ccctcgaata tggcccgaga cgacggagac
     2161 cgccccgaat tctcccatcg cccgcgcgtt gcagaggatg acgccgtaga gcaggctcca
     2221 cttgatattc gggagcgtga cacggataaa ggtttgccat cccgacgcgc ccaacgtgaa
     2281 cgcggcctct tcctcttccc gtccttgcga ttgcatcagg gggatgagtt cgcgcgcgat
     2341 aaagggcacg gtgacaaaga tcgtggcgag cacgatgccc ggtaatgcaa agataatcct
     2401 gatgtcgtgc tcgatcagcc aggggcccac ccagccctgg gctccgaaga gcagcacata
     2461 aatcaatccc gagatcacgg gggacaccgc cagcgggatg tcgatgatgg tgatgagcag
     2521 ttgttttccg acgaattcga atttggcgat ggcccaggct gcggcaaccc cgaacaccag
     2581 attcaatgga acggccaggg atgcagcgat gaacgtgagg cgaatggccg ccagggtatc
     2641 cggatcctga aacggggcga agtagccctc aaggccgttt ttcaatgctt cggcaaagac
     2701 ggctgcgagc gggatgaata ggaagagcgt caaatatcca agagccaaag caatgagtgt
     2761 ccagcggacc cacgtgcttt cgccggtcaa tgaagccttc cattcctcgt ttcttacttt
     2821 ggccgcgatc tctattgaca tgtgggtttc tttctaacga gcggtgtggc gtgcgctgct
     2881 ccaccattga aggagattga tcatgaggac gagcagaaac gacgccacca gcatgacgac
     2941 tcccagcgcg gtcgctccgg catagtcgta ttgttcgagc ttcgtcatga ttagcaacgg
     3001 tgtgatctcc gatttgagcg gcatattgcc ggcgataaag atgaccgagc cgtattctcc
     3061 gacggagcgt gcgaatgcca tcgccatgcc ggtcagcagg gcaggccaca gttccggcat
     3121 gatcacggcc cggactgttt gccaccgatt ggctccaaga gtcgtggcgg cttcctccac
     3181 ctccttgtcc agatcttgta ggactggttg tacggttcgc acgacgaacg gcaagccgat
     3241 gaaggtgagt gccaccagga cgcccagggg cgtaaaggcg attttgagac ccagcggttc
     3301 gagatactgt ccgatccatc cgtttgacga atagatggcg gtcaaggtga ttcctgcgac
     3361 ggccgtcggc agggcaaagg gaagatccac gagcgcgtct acgatcgaac gcccgggaaa
     3421 gcggtatcgc acgaggaccc aggcgaccac cgatccgaag aagccgttga tcaaggcccc
     3481 caggaatgac gcgccgaacg tcagccgata ggaggccagc gcgcgtggct ccgtcaccgt
     3541 gtgccagaat tgttcccagg tcaggctgct cgtcttgacg aagaggccgc tgagggggat
     3601 caacacaatc aggctgaagt agaatagcgc aaatccaagc gtcagcccaa atccgggaag
     3661 gacgctcggc tgttttaaca ccaatgtcat tggtctcatc gacgttactt gggccccttc
     3721 aaatagatct gatcgaaggt gccgtcatcg gcaaagtgtg tcttgtgcgc cttctgccaa
     3781 ttcccgaaga attcttgcag cgtgaaccgt tccactgtcg ggaacgtcgt cgcatacttg
     3841 gcggcgactg attggagatg cgggcggtag tagttcttgc cggcaatgtc ctggccttct
     3901 tcagaataca gatattcaag ataggcttga gcgacttgcc tgctgcctcg cttgtccacg
     3961 accttgtcga cgagactgac agtcggctcc gccagaatac tgaccggcgg cacgacaata
     4021 tcgaacttgt cttttcctga gcctttggtg ctcaacagga tctcgttttc ccagttaatc
     4081 aggacgtcgc cgataccccg ttccacgaag gtggtcgtcg cgccgcgggc gcccgagtcg
     4141 agtaccggca cgttcttgaa gaggcgctgg acaaaatcct gcgcttttgt gtcatcgccg
     4201 cccggctgtt tcagcgcata gccccaggcg gccagatagt tccagcgggc gacaccggaa
     4261 gttttaggat tcggggtaat gacggagatc cctggtttga ccaggtcatc ccaatcctta
     4321 atgcccttag ggttgccctt gcgaacgaga aacacgatgg tcgagctgta gggtgcgctg
     4381 ttgtgaggaa aacggctctg ccagtttgcg ggaagcagcc cggctttttc tgagatggca
     4441 tcgatatcgt atgccaacgc caaagtcacc acgtctgcct ccaggccgtc gatcacggcg
     4501 cgcgcctgtt tgctcgagcc tccgtgtgac tgtttgatgc tgaccgattg gccggtcttg
     4561 gccagccagt gcttggcgaa ggccgcgttg aactcctgat agagttcacg cgtcggatcg
     4621 tacgagacgt tgagaagtga gatggattgt cccgcctgga tagacgaagc aaaccccacg
     4681 gctccggtca ggagcgcgag tgtgagaacg aacggcttga acatcatggg acgatccttt
     4741 ctcatgtaat gtgtgcgtca gggtttcgta gggagtttct ttccgtcgcg ctccgggctt
     4801 ggctgaacgg ttaatgcggc aagcgcatcc tgataccgaa tctgcaactc ttcacgctga
     4861 tccggcgccc agaaacgggc cgacgagctg gccttggcca gaaactcctc cggctcgaac
     4921 agcacgtcat atgatgccga ggtctccgga atgttcggga gttgccgttc atacttgccg
     4981 atgtcgggac ccgggccgtg ccggaggtgg acaccgctta ttgtcagata cagaattgag
     5041 cctcggatcg aaatacagcc cgtggtggtt tcctggtctc cctgatcagt ggcgtgactt
     5101 aaattgaagt acacacgttc ggtcggttca gcgagagaca gggccgttga aagaggcccg
     5161 gccaacacag cgatctcttc tttccggaat gccctggttg gatccgcttg accgaacagt
     5221 aggcggtgca cgatgttccg ccgttccacc gcgcggactc cccggagcag ggtgccgacc
     5281 tctgccggcg tcagtgtgac gggatgcgca ttggatgatg agtcaggatc ctcctcaacc
     5341 cggacggtat tcagtcccgc ctcaaggatt gttctcaccg acgtgacagg ctggcctgcg
     5401 caaccgatcg tgaggaatat accgacgatg atcaacggcg cgcgcagcgg ccatgcagac
     5461 ggttgtgccc aattgttttg gtcttgattc attattgtcc ctcctgtcgt tgagcgacac
     5521 cgacttgcgg agcgatacga tcaaagccgt ggtcctgcaa gagggcttgt gccttgggtg
     5581 tctccaaaaa attgatgaaa tcccctgcgg ccgggatgtt cttggcggtc cagacggcgg
     5641 ccactccgta gtggaccggc tggtgcgagc ccgccggggc ctcgtcgaga atacggatct
     5701 tcttattcga tacggcgtct gtgcggtaca cgacgccgag atcggcttcg cctttggcga
     5761 ccaggtccag gacggctcgc gaatgctcgc catagacaaa ttgcgatttc agttgggaat
     5821 cgagcttgct gtaattgaaa tactgagccg ccactttccc taccgccgag gttttcgggt
     5881 cgccgacggc gatataccga accagggttg tgcgaagatc ctggattgac gtgatcggag
     5941 ccggaaactc agggccggca atcagaacca acgaggtgcc ggcatagaca cgttttgtgc
     6001 cttgaatgac gagccctttc ttctcgagct ggtcgatgtc ttctacctgc gaaggtagaa
     6061 acacatcgac cggtgcgccc tcttcgattt gtttgatgag agttttcgat ggcccgtaga
     6121 tgaccctcac gttgatgtcc ttattttgtg cttcaaagag cggaaggagt tgtcgaaatg
     6181 catcccgcag actattcgcg gccgcgatgg tgagggtctc gggtgccgcc tgcacggacg
     6241 gcgctgtcag aatgcacgtg gtcaacgcag cgaagagcaa cagacttctc aagactaggg
     6301 ttcggcctgt cataacgtac ctcctggtca tcgtaataaa gttcagctat aggcttagaa
     6361 ataaaattgg tactgcacat tcacccggtt ttcgatgaga tcgctgccga atcccggctg
     6421 gttctcgaac gggaaccggt ccggtgcacg tccgccgctt cccactgtga agttggagta
     6481 gtcgacgacc aatcggttat tgtaggtgcc atcgaagctg tagttgatcg aggctccgaa
     6541 ctccttcgtc agatcatttt gctgcttggt cgagggatcc aggaccccgt agcgaacggc
     6601 cagttcgagc ttgcgaggga tgacatattt ccccacctgc acataccagc cccaggcttg
     6661 gccgagatcc accggagggc cgaccgccag tgggcttgtc gggattgttg agagatcgaa
     6721 cggggaggtt ccactatcac tgtttctcac gcgctggtgg cgatagtagc cttcagcttg
     6781 gagggaccat ccttggtact tcacgatgaa atccgtttca taggtctgga aatcgtagat
     6841 gcctccgccc agaagcctcc cattctgtgc cgcggtcaca gcctggcggt atgagcggtc
     6901 cacaatgtcg gagcggatgg cactcagata gttttgagcc gggtcgtagg catagccgaa
     6961 ggcaatggcc gcttgcggcg tgcgcgagtt gagaatgtct ccctggccat agcctggatt
     7021 acccgcgatt ttatacagca aacgggccgt atacatgatc tcaccgctca tgaagcgagt
     7081 gtcatagtta taggttcggg tggtgcctga cggcagcgtt cctgccccat tagaaggcgg
     7141 cagcacgttc tgactgacgg ccgtaccgtc tcgcgcaagg tttggtccga ctccccccca
     7201 gagaccaaag gcgtaattga atctgtattg atcctcattg ctgaggatgc tgattccgac
     7261 gtcgcggctg ttctgaagat tggacgcaaa catattctgt acaatcatgt tatcggcaaa
     7321 ggttgacgtc gcgatcgagc taatgagagc acggttgaac cagaccgtct gttggccaac
     7381 ttgaaccgtt gcccagggga tatgccagga agagatatac gcatcttgga gtgcggctcg
     7441 tccgttgcct ccctcttggt tccaaggagt cgagtccatg gccacactga cgttgtagcg
     7501 caagtcggga tcgaaggcgt gcccgagaaa ttggaggcga acacggggaa cggtaaactg
     7561 actcgaatcg ctctggcgtc taaaggcacg aaactctacc tggcctccta aaatttctgc
     7621 attcctggaa ttcccgaccg tcccccaggc gtcattgaac gtgctgtagc tgtagcgtgc
     7681 ctgcgcgagc agtctcactt tgaggagaaa tttctccccg acgcgaatgt ttaatccgtt
     7741 attcacgtcg atgttaaaat tcggttttgc gatacgctcc ttggtctcca acaccttgag
     7801 ccccttgttg tattcgtctt cgttaatgat tcccttgagg tacaagtact tcaagccggg
     7861 atcttcgccc gcatagtatt cccacttgcc atctttctta accagctgcc cctcctcgac
     7921 ggcctgtact gacgggacgc gcgtgaatgt gatcgtcatc atgagtaaaa agagtgcacc
     7981 gaaaattctc atgtcgtttc cctccatgac atgggaactt ccggtggtcc ggtgagttct
     8041 cagattgatt aatgatggtg tcttgggcct ccagtggtct ggacggcagt atgtcctccg
     8101 ttaattgcta caacagtact gtgtaggcac attcacctcg ctcgttgagt tgtgtgtgca
     8161 gggtccgtgt ccaagcggat cttttcttta cgttcgatct gcacgatccg ggaatcgtgg
     8221 ataatgatct cgacggatcc gtagcggatt tctcgaatgg cgcgaaggat cgtattcgcc
     8281 agatgatggt ctgtttcatc gaggagtccg ttgatgtgtg gcatacgtga ttccatctat
     8341 ccctactgtt ccgatcatga atatgtatga tggttatata atagtatagt ttctttgtca
     8401 acaatataaa tttaagaata agattcacga taaaaaatta tttgaaaacc atcgcataga
     8461 caattattat atttaaattc aatagtttat agatgtgatg cttttcacat atccgtaaat
     8521 gtttgacatg ttgcgtcttt caatattcca agtcgcaggc ctaaacaatt cgtaagtttt
     8581 ctaatcacct caaaatgatg gatcattcaa ctcaaacagc gcttctggta tttgggtcag
     8641 aacaaggaca ctttcagaat gtcccgtttg agagattcca ccgggcaatc tggaaacgat
     8701 atcgggcatg atgtggaact tcgtgtgcaa tgggaggtca acagcaatgt ggattttgat
     8761 gcggggtacg agcattggtt caagggatcc tactttgacc ggctccccgc tggcaccggt
     8821 ttacccgctg gtggaaacaa ggacacggat ttcttctatc ttcagatgag agtcaggctg
     8881 tagagaaagg cgctggtcct attagacgtg gcgatcgggc gaggcattct gttggtgaaa
     8941 ttccacggat aggcaatcgt gaagcactat ggatgtacgt gacccagtta agcgatggac
     9001 aaaggtcgtt gg
//
LOCUS       DATIPF010000120        10019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1437625, whole genome shotgun sequence.
ACCESSION   DATIPF010000120 DATIPF010000000
VERSION     DATIPF010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 10019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10019
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437625"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VLL06_03055"
     CDS             complement(<1..356)
                     /locus_tag="VLL06_03055"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29975.1"
                     /translation="MKRPIRQSTRKPVIGVMGPAKAGIQELADAKSLGELIARQGWVV
                     LTGGRASGVMDAASEGAKSVPGSLTIGVLPDGKAQVSRYVDVAIVTDLGQARNNVNVM
                     SSDVIVACGLGGTGTVS"
     gene            complement(485..790)
                     /locus_tag="VLL06_03060"
     CDS             complement(485..790)
                     /locus_tag="VLL06_03060"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HSS29976.1"
                     /translation="MAQHRSGHTPNAQFCDYGNEYRTAIFFQTDEEKRLSDASKLSIE
                     QSKRFKEPIVTQIVMASQFYPAEEYHQDFYKKNPVRYKFYQYNCGRAKRLEELWGKH"
     gene            1219..1494
                     /locus_tag="VLL06_03065"
     CDS             1219..1494
                     /locus_tag="VLL06_03065"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HSS29977.1"
                     /translation="MPLPSYRHNGKRRARGLGSTAITVPRLSDAVVSVQTSTSTLDVV
                     RQLLSFSQTQDLSYDITGRLHLKDGHLPFDASGILLEKGQLSGAPSP"
     gene            complement(1499..3136)
                     /locus_tag="VLL06_03070"
     CDS             complement(1499..3136)
                     /locus_tag="VLL06_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29978.1"
                     /translation="MFATFLVLGMTCWLAMVVPVQAGTTDQADAPPLFDNLGSLHHPI
                     TTSSDLAQQYFDQGLRLVYAFNHEEAIRSFEAAAQEDPQAAMAYWGMALALGPNINSA
                     MEKKDERRAIEMVQKARRLADRAMPEEQAYIDALVTRYVSRKGAKRKGLDEAYAKAMR
                     LVAQRFPEDADAATLFAEALMDLRPWDLWKPDGRPQPGTDEIVTTLESVLAQNPEHPG
                     ACHYYLHAVEASLQPERALPCAERLPALMPGAGHLVHMPAHIYMRLGKYHEAVERNQQ
                     AADIDQQYLASRNQAGRYANGYYTHNLHFLWASLAMEGRNVEAMKAARDLTATITMEE
                     ASKDRWKERYLATPTFSMIRFGRWEELLKELPPPKGLRLMEGMWRLGRGLALVATGRL
                     PGAEGEHVVLAGLTKQIRRDRTTEEKTDRALLKIAERVLAGELAAHRQHYNDAIRLLE
                     EAIKMEEALPFSEPPLWPLSVRHHLGAVFLLADRPAEAEAVYHADLLQHPENGWALIG
                     LIQSLRAQQKDDQAAEAEDRFKKAWAHADFIPAASRM"
     gene            complement(3199..3576)
                     /locus_tag="VLL06_03075"
     CDS             complement(3199..3576)
                     /locus_tag="VLL06_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29979.1"
                     /translation="MPITRWCLISVLGASLLGAGCNIAGTSTQEDLGRGIVRGTLGAQ
                     PAGDVRLARPANAVSFTGQVARVEGGAYVIQEVSGLERRVAHDENTRIDRPAHVGDWI
                     EVFVDGTGRAVLIRNIDHDEQLQ"
     gene            complement(3669..5012)
                     /locus_tag="VLL06_03080"
     CDS             complement(3669..5012)
                     /locus_tag="VLL06_03080"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HSS29980.1"
                     /translation="MSTTGHKTWPKLTNRDEFRFIAEQILKRSTGDHTLVALHDQQSG
                     TTRFANNQIVQNVDTRRGALSVTVAFGQHHGTAVTTDFTAGSVQETVTRATEIAHLSP
                     EDPEYLTPVGPQQYLTPPTMRAETAAAGPVRRLEYTNEAIGQCRMENLGAAGIVSSSI
                     ATVGVAASTGLFAHEERTDARFSLTAQAGESTGWSAAAHRSIDHLRVQERTLTAIDKA
                     KRGLDARELPPGCYTVILEPAAVAGLWSSLVWMLDAKSYEKGTSAFSGKLGKSIVDKR
                     LTLRNLPAHDDLFGTGFTSDGLPTVASDWITEGVLTQLAYDRFTAKMRGIDIIPTLDA
                     PYLSGRGSSLPTLQDMIKNTERAILVTNFWYIRTVNPTDLTLTGMTRDGTFLVEKGAI
                     VSAVKNFRFHESPLRAFNRIEALTNPAEAITSETGKFLVPAMTLRDFNFSSVTRF"
     gene            complement(5009..6475)
                     /locus_tag="VLL06_03085"
     CDS             complement(5009..6475)
                     /locus_tag="VLL06_03085"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HSS29981.1"
                     /translation="MPAHLNPHFDELAEVALKCVVASGAEYGDIRLLDSRTQTIRGED
                     RRIASIRDTEDRGLGIRVLYHGAWGFAASSVLSLEEAPRVAELAIEIAKGSASLASEK
                     VKLADEPVHRDRVVTVRRIDPFAVALETKTALLLETMEYVHRQPGIVRSSASLWARRD
                     RKLFVSTEGSHLEFDLLALSGDFDATAVHDGRFASRSFNTPHLRKGYELIENANFLAE
                     APRIASQAVEKVKSPVTEPGLYDLVLDPEHLSLTMHESCGHPSELDRALGYEANYAGT
                     SFLTTEKRGTYRYGSPHVNFVADNTEPETLAATGYDDDGVACQRWDIVRGGIFVDYCT
                     NREVAPKIGETRSRGSNRADSWGSVPIVRIANICLEPGAATLDDLLADVKRGIYIEGH
                     GSFSIDQRRYNFQFGGDAFWLIENGKRTHMVRDVIYHGITPEFWNSCDGVADRSHRRR
                     YGFITCGKGQPGQSGWMTHAASHARFRNVKVIGGQSGS"
     gene            6578..6889
                     /locus_tag="VLL06_03090"
     CDS             6578..6889
                     /locus_tag="VLL06_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29982.1"
                     /translation="MVPLERTPRQRIIELITGTRLSSHQLAHMLGIPERQVEEHLTHV
                     VKTILRDKTRQFILDPARCQDCDFVFRNRSRLTSPSRCPHCRSEAIAPPRYGINLTVS
                     S"
     gene            6910..7170
                     /locus_tag="VLL06_03095"
     CDS             6910..7170
                     /locus_tag="VLL06_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS29983.1"
                     /translation="MVIEAKNEIGETMRQWMQIGAVVFCVALAACNGDKPKELFETAE
                     LEERQHNVAHAKQLYEDLVRLYPSSPQAETARARLVLLNNTP"
     gene            7354..7707
                     /locus_tag="VLL06_03100"
     CDS             7354..7707
                     /locus_tag="VLL06_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29984.1"
                     /translation="MAKPYAVRVGQRISVRYPFLYHGEGFHGKGQLWDLSASGWRATG
                     DYPVTQGMVMPVYIELPHEEGESTYLLIESAIVRWSNGRDAGWEIQTMDKSSRARLNR
                     FLDKGSSESARTTSR"
     gene            complement(7724..8518)
                     /locus_tag="VLL06_03105"
     CDS             complement(7724..8518)
                     /locus_tag="VLL06_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HSS29985.1"
                     /translation="MTTDSFTLVLTVYLVMVAVMVGLWVLQLRVRNVSIADVGWCAGL
                     IAVVLWYATQATGDTERKTLVAALAVIYAGRLGLYLLLNRVIGKSEEARYRRLREQWG
                     SSESVKMFGYFQMQALAVVLFSLPFLVVIQNPQPPFVLIDLVGLLIWVVAVFGETMAD
                     LQLAQFRSKAWNRDRVCRDGLWRYSRHPNYFFEWLHWWTYVVMGVTTPGWFLTWIGPI
                     GMGWALLKVTGIPWVERQALASRGEDYREYQRTTSAFVPWFPRDSR"
     gene            complement(8515..9390)
                     /locus_tag="VLL06_03110"
     CDS             complement(8515..9390)
                     /locus_tag="VLL06_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSS29986.1"
                     /translation="MAPRAIITGAAGLIGQYLVKTASRWAHDWEVQGLTRADLDLTDH
                     ASTDRTWQSIKPDAVIHCAALSRTKDCEQDPESARRINVEVTAHLAQLSRHIPFVFLS
                     SGEVFDGRTGWYGETDTPNPINVYGQTKMEAEQAVLQNPLHAVVRIVLTAGTSETGNR
                     SFVEDMCRTAQAGKEVTLYADEFRCPLPAGVIARAIWELVDCKQPGLYHLGGSERLSR
                     WEIGESLLPWYPELKGRLVEDSARNHIGSLRPVDLSLRYDKIQSLLSFRIPGFREWLT
                     GRSRRDADLWDYIAP"
     gene            complement(9401..>10019)
                     /locus_tag="VLL06_03115"
     CDS             complement(9401..>10019)
                     /locus_tag="VLL06_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSS29987.1"
                     /translation="IVGVIGPNGAGKTTMFRMIIGKEKPDAGTIKIGETVKLGYVDQD
                     RSLDPNKSVYDIISEGHETIQLGKAEVNARAYCARFNFAGTDQQKKVKDISGGERNRV
                     HLARMLKEGANLIILDEPTNDLDINTLRALEEGLESFAGCAVISSHDRWFLDRIATHI
                     MAFEGDSKVVWFEGNYSEYEADRKKRLGKEADQPHRIRYRRLTRQ"
BASE COUNT         2047 a         3041 c         2765 g         2166 t
ORIGIN      
        1 gaaaccgttc cggtaccgcc gagcccacaa gccacaatca cgtcactgga catcacgttc
       61 acattgttgc gagcctggcc caggtccgtc acgatcgcca cgtccacata tcgagacacc
      121 tgggctttcc catcaggcag aactccgatc gtcaagctgc cgggcacaga tttcgccccc
      181 tcgctggccg catccatcac accgctcgca cgccctcccg ttaatacaac ccatccttga
      241 cgtgcgataa gttctcccaa gcttttggcg tctgccagct cttgaatacc agccttagcc
      301 ggacccatga cacctatcac cggttttctg gtcgattgtc ggatcggacg tttcacagac
      361 cgcacctcca gggacaatgg cattggcaca ggttgctagg atcagaaata tacaccgagg
      421 gcggggggct tgtcgcgcga ctcttgcgaa gtatccaaac gagaagagag gacgcccgct
      481 taggttagtg ttttccccat aactcttcca accgtttggc gcgaccgcaa ttgtattggt
      541 agaacttgta gcggactggg ttcttcttgt agaagtcctg gtggtactcc tcggcgggat
      601 agaactgcga ggccatcaca atctgggtga cgatcggctc tttgaaccgt ttcgattgct
      661 caatggacag cttggatgca tccgacagac gtttctcttc gtcggtctga aagaaaatag
      721 cggtgcgata ctcgttgccg tagtcgcaga actgtgcgtt tggtgtgtga ccggatcgat
      781 gttgtgccaa taggcatcca gtaacttttg gtaactcact ttggttgggt catagatcac
      841 ttcgaccgat tccgcgtgcc ccgttcgtcc agcggacacc tcttcatagc tcggattggc
      901 gaccgtacct cccataaacc ccgatgtcac agacagcacc ccctcaacct tctcgaaggc
      961 gagttccatg ctccagaaac agccccctgc gaagtaggcc ttgccgggat tctcggccgc
     1021 tacagtctgg ctagtcgatt ggacgcttaa agctccgatg aggaacatgg ccagagtaag
     1081 aattgatgga gccattcgtc ttctcataaa agggccctcc tcactgattt ggtcgaatcg
     1141 cccagtgaaa cctttcacct attctacggg cttagtttgg attcggatgt tccgtaggat
     1201 cgagagccat gtggaccgtt gccccttccc tcctatcgcc ataacgggaa acggcgggcg
     1261 cgcggattgg gcagtacggc catcacggtc ccccgactca gcgatgccgt tgtctccgtt
     1321 cagacaagta cctcgacctt ggatgtcgta cgccagctcc tgagcttctc ccaaacgcaa
     1381 gacctctcgt acgacattac gggccgactc cacctcaaag acggccacct gcctttcgac
     1441 gcttccggca tcctcctcga aaaaggccag ctctccggcg caccctctcc ctagtccgtc
     1501 acatccgaga cgcagccgga atgaagtcgg cgtgggccca ggccttcttg aagcgatcct
     1561 cggcctcagc cgcctgatcg tccttttgtt gggcccggag gctctggatc aatccgatca
     1621 gcgcccaccc attttccgga tgctgcagaa ggtccgcatg gtagaccgct tctgcttcgg
     1681 ccgggcgatc tgccagcaag aacaccgccc ccagatgatg ccgcacggac aacggccaga
     1741 gcggcggctc cgaaaatggg agtgcctctt ccattttgat cgcttcctca agtagccgga
     1801 ttgcgtcatt atagtgctgg cggtgcgcag caagctctcc cgccaggacc cgctcagcga
     1861 ttttgagaag cgcccgatca gtcttttctt ccgtcgtgcg atcccggcgg atctgcttcg
     1921 taaggccggc aaggacgaca tgttcaccct cagcaccggg gagccgtccg gtcgcaacaa
     1981 gggcgagccc gcgccccagc cgccacatcc cttccatcaa tcgcaatcct ttgggcggcg
     2041 gaagttcctt gagcaactcc tcccaccggc cgaaccggat catcgagaaa gtcggtgtcg
     2101 cgaggtatcg ttccttccag cgatctttgc tggcttcctc cattgtgatc gtcgccgtca
     2161 agtcgcgcgc agctttcatg gcttcgacat tacgtccctc catcgccaag gaggcccaga
     2221 ggaaatggag attgtgcgtg taatacccgt ttgcatacct gccggcttgg tttcgacttg
     2281 ccaggtattg ttgatcgatg tcggctgcct gttgattccg ttccaccgct tcatggtact
     2341 tccccagccg catgtaaatg tgggcaggca tatggacaag atgaccggcc cccggcatta
     2401 aggcaggtag ccgctcagca cagggtaagg cccgttccgg ctgcaatgac gcctcgaccg
     2461 cgtgtaggta gtagtggcag gcccctgggt gttcggggtt ctgtgcgagc acggattcga
     2521 gcgtggtgac gatctcgtcg gtgccgggct gtggtcgtcc atccggcttc cacaagtccc
     2581 agggccgcag atccatcagc gcttctgcaa acagcgtggc cgcatcggca tcttcgggaa
     2641 accgctgcgc caccagtcgc atggccttcg cataggcttc atcgagtccc ttgcgcttgg
     2701 ccccctttcg gctcacatac cgcgtgacca acgcgtcgat gtaggcttgc tcctcgggca
     2761 tggcacgatc tgccagcctt cgcgcctttt ggaccatctc gatggcgcga cgttcgtctt
     2821 tcttctccat ggcactgttg atgttgggac cgagcgcgag cgccatgccc caataggcca
     2881 tggccgcctg cggatcttct tgggctgccg cttcgaacga ccggatcgct tcttcatggt
     2941 tgaatgcata gacgagtcgg aggccttgat cgaaatactg ttgggccaga tccgatgaag
     3001 tggtgatggg atgatggagg gaaccgagat tgtcgaagag cggtggagcg tcggcctgat
     3061 cggtagtccc ggcttgcacc ggtacgacca tcgcaagcca acaggtcata cctaggacca
     3121 agaatgtagc gaacatctat cctcctacta tggttcgcga tcgctcgttg ctcagcccat
     3181 atcacatgcc agctctggtt actgcagttg ttcgtcgtga tcaatattcc gaataaggac
     3241 ggcgcggcct gtgccatcga cgaacacttc gatccaatcc ccgacatggg ccgggcgatc
     3301 gattcgagta ttttcatcat gggccacgcg acgctctagg ccactgactt cctgaatcac
     3361 ataggccccg ccctcgactc tcgccacttg tcctgtgaaa gaaacggcgt tggccggcct
     3421 cgcaagccgc acatcaccgg ccggttgggc tcccagcgtg ccccgcacga tccctcgtcc
     3481 aagatcctct tgcgtcgaag ttcccgcaat gttgcaacct gcgcccagaa gactcgctcc
     3541 cagaacactg atgaggcacc accttgtaat aggcatacgc atcgagatgc tttcccgctc
     3601 ttgtctaccc ttgctcttcc tccgagtcaa caatccaata ggagattgtc gctcgtgact
     3661 gacgaacttc agaatcgggt gacgctggaa aagttgaagt cgcggagcgt catcgccggc
     3721 acgagaaatt tgcctgtttc ggatgtgatc gcttcagccg gattggtcaa ggcctcgatc
     3781 cggttgaacg ctcgcagcgg gctctcgtga aagcgaaagt tcttcactgc cgacacgatc
     3841 gctccctttt cgacaaggaa tgtgccgtct ctggtcattc cggtcagagt aagatcggtc
     3901 ggattgaccg ttcgaatgta ccagaagttc gtcacgagga tggcgcgctc cgtgtttttg
     3961 atcatgtctt gtagtgtcgg cagggacgat cctctgcctg agagataggg cgcatcgagc
     4021 gtgggaatga tgtcgatccc tcgcatcttc gctgtaaacc gatcgtaggc cagttgtgtg
     4081 agaaccccct ccgtaatcca gtctgatgcc acggtcggta acccatcact ggtaaacccc
     4141 gtgccgaaga gatcgtcgtg cgccggcaga ttgcggagcg tcagtcgctt gtcgacgatg
     4201 gacttgccca gtttgcctga gaatgcactc gtccccttct cgtaggactt tgcatcgagc
     4261 atccagacca gcgaggacca caaccctgcg acggcagcag gttcaagaat caccgtatag
     4321 catccgggcg gcagctcgcg cgcgtccaga ccccgcttgg ccttatcgat tgcggtgagt
     4381 gttcgctcct gaaccctcag gtgatcgatg gaccgatggg ccgcagcact ccagccggta
     4441 gattcgccgg cctgcgccgt gaggctgaat cgtgcatcgg tccgttcttc atgggcgaag
     4501 agccccgtac tcgcggccac gccgaccgtc gcaatcgatg aggacacaat cccggcggcg
     4561 ccaagatttt ccatccggca ttgcccgatc gcctcgttcg tatattccag tcgacggacc
     4621 ggaccggccg cggcggtttc ggctcgcatc gtcggaggcg tcagatactg ctgtggcccc
     4681 acgggcgtga ggtattccgg atcctccggc gacaggtgcg cgatctctgt ggctctggtc
     4741 accgtctctt gtaccgaccc ggcggtaaaa tccgtcgtga cagcggtacc gtgatgctgc
     4801 ccgaacgcca ccgtcacgct taaagccccg cgcctcgtat cgacgttctg cacgatctga
     4861 ttgttcgcga accgcgtcgt cccgctctgc tggtcgtgca acgcgaccag cgtgtggtca
     4921 ccggtcgaac gtttcaggat ttgttcggcg ataaaccgga actcgtctcg gttcgtcagt
     4981 ttcggccagg tcttgtggcc ggtcgtactc atgatccgga ctgccctccg atgaccttca
     5041 cgttgcggaa acgtgcgtgt gacgccgcgt gagtcatcca gcctgattgg cccggctggc
     5101 ccttgccaca ggtgataaac ccataccgac ggcggtggga ccgatccgcg acaccgtcgc
     5161 agctgttcca aaactccggt gtgatgccat ggtaaatgac atcacgaacc atgtgtgtcc
     5221 gcttcccgtt ttcgatcagc caaaatgcat cgccgccgaa ctggaagtta taccgtcgtt
     5281 gatcgatgct gaaggagccg tggccttcga tgtagatacc acgtttcaca tcggcgagca
     5341 ggtcgtcgag ggtggcggcg cccggctcaa ggcagatgtt ggcgatgcgg acgatcggga
     5401 cgctgcccca gctgtccgcc cgattggagc cgcgggaacg agtctcgccg attttcggcg
     5461 cgacttcgcg gttcgtgcag tagtcgacga agatgccgcc acgtacaatg tcccatcgct
     5521 ggcatgccac gccatcgtca tcgtatccgg tcgcggcaag ggtctccggc tccgtattat
     5581 cggccacgaa attcacgtgc ggagaaccgt accgataggt accccgtttc tcggtcgtga
     5641 gaaaactggt gccggcatag ttggcctcgt acccgagggc acgatcgagc tcgctcggat
     5701 ggccgcagga ctcatgcatc gtgagcgaga gatgttcggg gtcgaggacg agatcgtaca
     5761 gacctggctc ggtcacagga gacttcactt tctcaacggc ttgagacgcg atgcgcgggg
     5821 cctccgccag aaagttggcg ttctcgatca attcatagcc tttcctgaga tgcggggtgt
     5881 tgaagctccg cgaggcgaac cgtccgtcat gaactgccgt ggcatcgaaa tcgccgctca
     5941 gggcaaggag gtcgaattcc aggtgcgacc cttcggtcga cacaaagagc ttgcggtcac
     6001 gacgagccca caggctcgca ctgctccgca cgatgccggg ctgacgatgg acatactcca
     6061 tcgtttcaag gagcagggcc gtttttgttt cgagcgcgac ggcaaatgga tcgatccgcc
     6121 gcaccgtgac gactcggtct cgatggaccg gctcatcggc gagttttacc ttttcactcg
     6181 caagggatgc ggaacctttc gcgatctcga tcgccaattc agccacacgc ggggcttctt
     6241 ccagcgaaag caccgaactg gcggcaaagc cccaggcgcc atgatagagg acccggatcc
     6301 caagtcctct gtcctccgtg tctcgaatgg acgcgatgcg gcggtcttcg ccgcgaatgg
     6361 tttgggttct gctgtcgaga agacggatat cgccgtattc ggcccctgaa gccaccacgc
     6421 atttcagcgc gacctcggca agttcatcaa aatgaggatt cagatgggcg ggcatggtgt
     6481 ctgtatcctg acagaggtac cggcgccatt caacttacct tactattgaa ccgcttgaac
     6541 ttggtttttg tcacgcaatt ccctacgatg tcgcgtcatg gtgcctctcg aacggacgcc
     6601 gcgtcaacgc atcatcgaat tgatcacagg caccaggctg tcctctcacc aattggcaca
     6661 catgctgggc attcctgaac gtcaggttga agagcatctc acccacgtcg tgaagaccat
     6721 cttgcgcgac aagacgagac agttcatcct ggacccggcg cgctgtcaag actgtgattt
     6781 tgtctttcgc aaccgcagcc gtctgacgag cccaagccgc tgcccacatt gtcggagcga
     6841 agccattgcg ccaccgcgct atggtatcaa tctcactgtc tcgtcataag acgagacacg
     6901 cgaccaacga tggtgataga agctaagaat gagatcggag aaacgatgcg acaatggatg
     6961 cagatagggg ccgtcgtctt ctgtgttgcc ttagccgcct gcaacggaga taagccgaag
     7021 gagctgttcg agactgccga gttggaagag cggcaacaca acgtcgcaca cgcgaagcaa
     7081 ctgtacgaag accttgtccg tctctatcca tccagcccac aggcggaaac cgcgcgagcg
     7141 cgccttgtct tactcaacaa cactccgtag tatccctgtc ccggacattc ccaggtctct
     7201 ctcgcgcgca cattgctgct gttcaatctg taatggcgta tccttctcaa gaatattcgt
     7261 tcccccctta agggcccttg cgctagcgag cagccctgct atagtagcaa gtacgtacga
     7321 aaccaatcat ggtccatcac cggggaaggt gacatggcca agccctatgc agttcgagtg
     7381 gggcagcgca tttcagtccg ttatcccttt ctctaccatg gcgaggggtt ccacgggaaa
     7441 gggcaacttt gggatctatc agcatcaggc tggcgtgcca cgggagacta cccggtcaca
     7501 cagggaatgg tcatgccagt ctatatcgag ctgccccatg aagaagggga atccacatat
     7561 ttgctcatcg aatccgctat cgtccgatgg tcgaacggac gcgatgccgg gtgggagatc
     7621 caaacgatgg acaaatcttc gcgggctagg ctgaatcggt ttcttgacaa ggggtcgtcc
     7681 gaatcggcga ggacaacatc tcggtaacac gatacgtctg cattcaccgc gagtcccgag
     7741 gaaaccatgg gacaaacgcg ctcgtagtcc gttggtactc tcgatagtcc tctccccggc
     7801 tcgcaagagc ctgccgctct acccagggaa ttcccgtaac tttcaggagt gcccaaccca
     7861 tgccgattgg gccgatccag gtcaggaacc atccaggcgt ggtcaccccc atcacgacat
     7921 aggtccacca atgcaaccat tcaaagaaat aattcggatg gcgtgagtac cgccagagtc
     7981 catcccgaca cacacggtcg cgattccagg cctttgatcg aaattgtgca agctgcaggt
     8041 ccgccatggt ttccccgaaa acagcaacga cccagatgag caacccgaca aggtcgatca
     8101 gcacgaaggg gggttgtggg ttttggatca caaccagaaa cggaagagag aataggacca
     8161 cggccagcgc ttgcatctga aaatacccaa acatcttgac cgactcagaa gaaccccatt
     8221 gctcgcgcag gcggcggtag cgagcctcct ctgacttacc aatgacccgg ttaaggagga
     8281 ggtagagccc gagcctccct gcgtagatca cggccaaggc agccaccagc gtttttcgct
     8341 ctgtgtcccc ggtggcctgc gtggcatacc acagcacgac cgcaatgaga cccgcacacc
     8401 aaccgacatc tgcaatcgag acattgcgca ctcgaagttg cagaacccat aggcccacca
     8461 tgaccgcgac catgacgagg tacaccgtca agacgagcgt gaatgaatcg gttgtcatgg
     8521 cgcaatgtaa tcccacaagt ccgcatcccg gcgtgaacgt ccggtgagcc actcgcgaaa
     8581 ccccggaatg cgaaacgaga ggaggctctg aatcttatcg tagcggagcg agaggtcaac
     8641 gggcctgaga ctcccaatgt ggtttcgtgc ggagtcttcg acgagtcggc ccttcaattc
     8701 cggataccaa ggcaacaagg attcaccaat ttcccagcgc gagagccgtt cactcccgcc
     8761 cagatgatag agaccgggct gcttgcaatc gactaattcc cagatggctc gggcgatgac
     8821 acctgcagga agggggcagc ggaactcgtc ggcatagagt gttacctcct tgccggcctg
     8881 agcggtccga cacatatcct cgacgaaact cctgttgccg gtctccgaag tcccggcagt
     8941 cagaacaatt cgcacgacgg catgtagggg attctgcaag acagcctgtt cggcttccat
     9001 cttggtctgg ccatacacat tgatcgggtt cggtgtatcc gtctcaccat accagccggt
     9061 ccttccgtcg aagacttctc cgctggacag gaacacgaaa ggaatatggc gggacagctg
     9121 cgcgaggtgc gcagtcactt ccacattgat gcgacgggcc gattcaggat cttgctcaca
     9181 gtcttttgtc cggctgagcg ccgcgcagtg aatcacggcg tcaggtttga tggattgcca
     9241 ggtgcgatcg gtgctcgcgt gatcggtcag atcaagatcg gcgcgggtca gcccctgcac
     9301 ctcccaatca tgagcccatc tcgaggcagt cttcacaaga tattgtccga tcaaccccgc
     9361 tgccccggtg atgatcgcac gaggtgccat ggtgggtcac ttattggcgc gtcagcctgc
     9421 ggtagcgaat gcggtgtggc tgatcggctt ccttgcccaa ccgcttcttc cgatctgctt
     9481 catactcgct gtagttccct tcgaaccaga ccaccttgct atcgccttcg aaggccataa
     9541 tatgggtcgc gattcggtcc agaaaccagc gatcgtggct gctgatgacg gcgcagccgg
     9601 cgaaactttc caaaccttcc tccaatgcac gcagggtgtt gatgtcaaga tcgttggtcg
     9661 gctcgtcgag aatgatgagg ttcgcccctt ctttcaacat acgagccagg tgtacacgat
     9721 tgcgttcgcc gccggagatg tctttcacct tcttctgctg atcggtaccc gcaaagttga
     9781 accgggcgca gtaggccctg gcattgactt cggccttccc aagctgaata gtctcgtggc
     9841 cctcggagat gatgtcgtag acggatttgt tcggatccag actgcggtcc tgatctacgt
     9901 agcccagctt gaccgtctca ccaatcttga tcgttcccgc atccggcttt tctttcccga
     9961 tgatcatgcg aaacattgtc gtcttgccgg ctccgttcgg accgatcaca ccgacaatg
//
LOCUS       DATIPF010000121         5658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1173423, whole genome shotgun sequence.
ACCESSION   DATIPF010000121 DATIPF010000000
VERSION     DATIPF010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173423"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            998..1243
                     /locus_tag="VLL06_03120"
     CDS             998..1243
                     /locus_tag="VLL06_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29988.1"
                     /translation="MVIRKSEDDKAGPELQRTVLLYLAENGSTKWGALYSYFYQEKPD
                     EIGLALQDLTQWKHISVELDGATRITASGLGQLNSRP"
     gene            1718..2779
                     /locus_tag="VLL06_03125"
     CDS             1718..2779
                     /locus_tag="VLL06_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS29989.1"
                     /translation="MVTQTAKPSGNRHLLLTCESGKLIQFDTETCLPRTLSQGPSRDY
                     LFGIACRGSMLYFTGCTFIAAARIGSPDLELVKMVTPYKPLRGTYNRWMRYLWTKLGA
                     DSRVIPYGKPDLHQINIYGSTIYVTATSWNEIWLFDLDLNLKRRIRIQPYIRDYHHLN
                     NVFCDGKHFYVCLNRYERSGGFGGYAKFDQEWNEVERRALGWESHALTVINSEVVHLC
                     CSTDGIGKEIRHPRRAGMMINETLVFEYDPGKYYCKDFSMDDARIYVVGGENTPREKR
                     KEAAGVVFILNRNYELLQKCVIPGIGGFNGCRLPDLDYSNGAAPLSPASEILGESDHT
                     DGEPTAWRFSTIQFRSCDR"
     gene            2791..3432
                     /gene="cysC"
                     /locus_tag="VLL06_03130"
     CDS             2791..3432
                     /gene="cysC"
                     /locus_tag="VLL06_03130"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HSS29990.1"
                     /translation="MQNIATVQDAGTDQVRHVDSQGDLLAKHGGKGFTVWFTGLPASG
                     KSTLSKAAHQALLMRGIKTPELLDADEIRTNISKGLGFSKEDRITNILRIGWIAQLLT
                     KHQIPNLVACIAPYEQARKQVREMVEVVGGKGSFIEVFVDCPLEACVRRDPKGLYAKA
                     FAGEIEKLSGLNDTYEAPEHPELRLNTATHNIAEAVECLVEYLETNHLIEASK"
     gene            complement(3607..3683)
                     /locus_tag="VLL06_03135"
     tRNA            complement(3607..3683)
                     /locus_tag="VLL06_03135"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3647..3649),aa:His,seq:gtg)
     gene            complement(3771..4484)
                     /gene="pyrF"
                     /locus_tag="VLL06_03140"
     CDS             complement(3771..4484)
                     /gene="pyrF"
                     /locus_tag="VLL06_03140"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HSS29991.1"
                     /translation="MSTIIARDRLILALDVPSSDEADRLLDRVQDQIVFVKVGLELYT
                     AAGPEIVHRLITQGKRVFLDLKFLDIEETVRRATARVAAMGVEFLTVHANRKALAAAV
                     QGRGESGLKLLAVTVLTNFDSQDLREMGIQRSVKDLVTARALLASEVGCDGVVASGEE
                     PAILRPKVGPRFLIVTPGVRPAGKDVDDHARATTPTQAISAGADYLVIGRPIRDAGDP
                     AVAAAAILTEMQTAFDARG"
     gene            4571..4849
                     /gene="rpsT"
                     /locus_tag="VLL06_03145"
     CDS             4571..4849
                     /gene="rpsT"
                     /locus_tag="VLL06_03145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HSS29992.1"
                     /translation="MAVVHKSTIRRARQSVKRHDRNRATLGALKSLIKKVQAAVAEKK
                     VEDAQVSLRQATSALGKAVTKGVMKPNTASRRIARLTLHVNSLSASPS"
     gene            complement(4821..>5658)
                     /gene="holA"
                     /locus_tag="VLL06_03150"
     CDS             complement(4821..>5658)
                     /gene="holA"
                     /locus_tag="VLL06_03150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HSS29993.1"
                     /translation="RECDAILPYLKEPNNSTAMIFVAPKLDGRLKFTQALTRVAVMVD
                     CSPLREAQWSPWLRQDAERVGIRLNEEAIELLKETCGSSLYSVRRELEKLAAYVSPGQ
                     TVTATEVATLRGTEPGASVFDLTLAIGGRHRGRVLAILARNLEAGEAPLRILGSLAWQ
                     YRRLWRVKEVVRYGGREGEAARTLRMDPYKVRAFLEQFPDAHLHEALRLFLEADAKLK
                     GGSGGRPARILEDLLLRLCDRVQSNAPPPSPSRGTEAPQPVKARVISNVRTITRGKQT
                     RS"
BASE COUNT         1367 a         1544 c         1494 g         1253 t
ORIGIN      
        1 gcgacttcgg cttcacgcaa tttggcgagg atctgttcga cggtatgacg cagatggcat
       61 gcagccctcc tttgggcccg accctaacat acagggtgga ccggtttaag gggactaggt
      121 cactcccgct ctcgaagata atcctggtcg gtcggaaggg gacctcaagc ggtattggca
      181 cgagacctac ggcgtgttag gggccagcga aaagaatctc aaatagaacg gtgggtcgtg
      241 acgtcgtggc atacaatctt caggatcctc ataacgcact ccctatttac attcaacggc
      301 ccaagggtgt tcttcttgag gagggatgcc acctttctct gttaccacga gcatcgaagt
      361 cgccgcgtca aaggtgtcat agccattcgg acagagttcc gcagcttttc tctgacattg
      421 atccaacgaa cgtccgaagc cgctacattc cataatatag gcctgtcttc cgtgcggtcc
      481 atgaaccggc tcatggttga cagacaggca agaggcaagg gacagcagca ggaccactag
      541 acagagcctt tgcacagcca tcatcctttc atcgtctgga gcccacgcgt atatttcttc
      601 aggaagtctc gaagatagcg agtggaagca ttctacgcaa aagttgaatc tgggcgcaac
      661 aggatgcggt gacccgtgat atctgggacg tgctgcaatg tattgagggt caatccttcg
      721 gcgccgccaa tatgagcccg aagcgttttg acgggtgact gatgacgcta ttacatagac
      781 ggtcttgtgg aactaggcaa ttactaggtt tcgcgggact gcttcctcga cgcccagggt
      841 ctcgatctat tcgccagccc aatactaaga acaccatccc ccgccagcct tttcttctgc
      901 atgtagctag gtgcctacct agtttcgcgg acgctctgga cgtgtacagt acccacgaca
      961 aggctcaaga cgagaatgaa cccagggttg aggcgccatg gtaatccgaa agtctgaaga
     1021 cgacaaggct gggcccgaac ttcagcgtac agtcctgttg tatctcgcgg aaaacggctc
     1081 aacgaagtgg ggtgcgctct atagttattt ctaccaggag aaaccagacg agattggatt
     1141 ggctttgcaa gacttgacac agtggaagca catttcggtg gaattagacg gcgccacaag
     1201 aatcactgcc tcggggctgg ggcaactcaa tagcaggccg taggtcgata catcaggccc
     1261 cctcctctcg tggcgttacg tggaacggtg tcaaagccga cattgtcacg acatcgatga
     1321 cttccatccg ctgcttgcga tcggcgtcat cacgatcaac ttctgttcgg cgctccattg
     1381 ccctcccgcc cagtccgaac agatttcact acaatgtcca tggctcactc ctcgcataga
     1441 ggacttgtct acattttact ggacacacca gggaccggta ggctgtcagc ttgaacaacg
     1501 tttttctttc tagaaactat tttttgtaac cttttatatg cagcatccga cttctagata
     1561 tagacatcat cacaatttga atagcggcaa acgaatgatc caacagtaga gaaggtaaag
     1621 aagaggcctg tgtacttccc gtcactatat ttatggagaa tctcacacct catgcgtgct
     1681 caagatcatt atgatgtgcg gtcataggga ggcctcaatg gtaactcaaa cagcgaagcc
     1741 ctcagggaat cgccatctgc tcctgacgtg tgaaagcggg aagttgatcc agttcgacac
     1801 ggaaacctgc ctgccccgaa cactatctca gggtccgtca cgagactacc tgttcggcat
     1861 tgcatgccgg ggaagcatgc tctatttcac cggctgtaca ttcatagccg cggctcgtat
     1921 cggatcacct gacctcgagc tcgtgaagat ggtgacgccg tacaagccat tgcgtggaac
     1981 atacaatcgc tggatgaggt acctatggac caagttagga gccgactcac gagtcatacc
     2041 gtatggcaag ccagatttgc atcaaataaa tatttatggc tcaaccattt atgtaacggc
     2101 gacctcttgg aacgaaatct ggctcttcga tctcgacctc aatttgaagc ggcgcatcag
     2161 gatccaaccg tatattcgcg actatcacca cctcaacaat gtgttctgtg acggcaaaca
     2221 tttttatgtc tgcttgaatc gctatgagcg atcaggcggg ttcggaggct atgccaagtt
     2281 cgatcaggag tggaacgaag tcgaaaggag agccctagga tgggagtcgc atgctctaac
     2341 tgtcattaat agtgaggtag ttcacctttg ttgttctacc gatggcattg gaaaggaaat
     2401 tcggcatccg cgtcgtgccg gcatgatgat caacgaaaca ctcgtgtttg aatatgatcc
     2461 aggtaaatac tattgcaaag acttctcgat ggatgatgcg cgtatctatg ttgtcggagg
     2521 cgaaaacaca ccacgcgaaa agcggaaaga ggcggccggg gttgtgttta ttctcaaccg
     2581 taattacgag cttcttcaga aatgtgtcat acctggtata ggagggttca acggatgccg
     2641 actacccgat ctggattata gtaatggggc ggcgcctctt tcgccggcaa gcgagatctt
     2701 gggcgaatca gaccataccg atggagaacc aactgcgtgg agattctcca cgatacagtt
     2761 caggtcctgt gatcggtgaa tggagtgtct atgcagaaca tcgcgacggt tcaggatgca
     2821 gggactgatc aggttcggca cgttgactcg cagggagacc tccttgcaaa gcacggcggc
     2881 aaagggttta ccgtctggtt tacagggctt cctgcaagtg gaaagtctac cctctccaag
     2941 gcagcccatc aggcgcttct gatgagaggg attaagacac cggagctcct cgatgctgac
     3001 gagattcgca cgaatatatc gaaggggttg ggattctcca aggaagatcg gatcacaaat
     3061 atcctgagga ttggctggat cgctcagtta ttgacaaaac accagatacc caacctggtt
     3121 gcctgtatcg ccccctatga gcaggcgaga aaacaagtca gagaaatggt ggaagtagtc
     3181 gggggaaaag gcagcttcat tgaggtcttt gtggactgtc ctctcgaagc atgtgtccgc
     3241 cgagacccca aagggcttta tgccaaagcc ttcgcaggag aaatcgagaa gctctccggg
     3301 cttaatgata cctatgaagc accggaacat cctgaactcc gattgaatac ggcaacgcat
     3361 aatattgctg aggccgtaga atgccttgtg gagtatctgg aaaccaacca cttaattgag
     3421 gcctcaaaat agaagaaagt gtaaaagaca gttggctccc gccagctcta catccggcag
     3481 agacagaaca ataaaggccc ggtcccattc gcgggactga gcctttattc agattccact
     3541 atggttgcac tgcatgcaca cggaggcggt atacctgcac ggggctttgg aatatcagta
     3601 agggaatggg gtggatgacg ggtttcgaac ccgcgacctc cggatccaca atccggcgct
     3661 ctaaccaact gagctacacc caccattctg agatgaaaaa cgacccggcg tgagaaggat
     3721 tgtggatctt agcaaagaaa gaagaaagcc cgcaacccaa tggaaacgcg tcaccccctg
     3781 gcatcgaagg ctgtctgcat ttccgtgaga attgcggcgg cagcgacagc gggatccccg
     3841 gcatccctga taggtctgcc gatcaccaaa tagtctgctc ccgctgaaat cgcttgggtc
     3901 ggtgtggtgg ctcgtgcgtg atcgtcgaca tccttgccgg ccggacgcac acctggtgtc
     3961 acgatcagaa atcgtggccc gaccttggga cggaggatcg ccggttcttc acctgaggcc
     4021 acaaccccat cacaacccac ctcggacgcc aggagagcac gcgcggtgac gaggtccttg
     4081 acgctccgct gaatgcccat ttcacgcaga tcctggctgt caaagttcgt gagcaccgtc
     4141 actgccaaca gtttcagtcc ggactctccg cggccttgga cagctgcggc cagcgctttc
     4201 cggtttgcat gaaccgtcaa gaattcgaca cccatcgccg ctacccgcgc cgtggcccga
     4261 cggaccgttt cctcaatatc gagaaacttg aggtcgagga acactcgctt accttgcgta
     4321 atcaggcggt ggacgatctc cggtccggcg gcagtgtaga gttcgagtcc aaccttcaca
     4381 aagacgatct gatcctgcac gcgatcgaga agtcggtctg cttcatcgct tgagggaaca
     4441 tcaagggcca atatgaggcg gtcacgggca atgatagttg acaccggttt gtccttttga
     4501 ggattggttc accttgaccc ttcactgagc catatggtac acatgtcttc tttttctaga
     4561 ggagtctact atggcggttg tacataaatc gacgattcgc cgagcccgcc agtccgtgaa
     4621 gcggcatgac cgtaaccgcg cgaccctggg tgctctgaag agcctcatca agaaagtcca
     4681 agcggccgtc gctgaaaaga aggttgagga tgcgcaggta tccctccgcc aagccacgtc
     4741 ggcactcggc aaggcagtga ccaaaggtgt gatgaaaccg aacacggcct cgcgccggat
     4801 tgctcgtctc acgctccacg tcaactcctt gtctgcttcc ccctcgtaat cgtacgcacg
     4861 ttcgatatca ctctcgcctt caccggttgt ggcgcttcag taccacgact cggtgagggt
     4921 ggaggtgcgt tcgactgcac ccgatcacat aaccgcaaca acaggtcttc aagaattcgt
     4981 gcagggcgtc caccacttcc acccttcagc ttcgcatcgg cctccaagaa tagccgaagc
     5041 gcctcatgta aatgggcgtc aggaaactgc tcaagaaacg ctcgcacttt gtagggatcc
     5101 atccgcaatg tccgagcggc ctccccctcg cggcctccgt atcttacaac ttccttaacc
     5161 ctccacagtc tccgatattg ccaggccagt gaaccaagga ttctcagcgg agcctcccct
     5221 gcttcaagat tcctggccag gatcgccaat acccgtccgc gatgtctccc cccgatggcc
     5281 aacgtcaaat caaagaccga tgcaccgggc tccgttccac gaagtgtggc cacctcagtc
     5341 gcggtgacag tctggccggg cgagacatag gcagccaact tttccagttc acgccgaacc
     5401 gaatagagcg agctaccgca ggtttctttg agcaattcga tggcctcttc gttgaggcgg
     5461 atcccgaccc gctcagcgtc ctgtctgagc cacggagacc actgcgcctc tctcagaggt
     5521 gagcaatcga ccatcacggc aacccgtgta agagcctgcg taaacttgag tcgtccatca
     5581 agttttgggg cgacgaaaat catggcggtc gagttattcg gctccttcag atagggaagg
     5641 atcgcgtcgc actcccgg
//
LOCUS       DATIPF010000122         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1084470, whole genome shotgun sequence.
ACCESSION   DATIPF010000122 DATIPF010000000
VERSION     DATIPF010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084470"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..718
                     /locus_tag="VLL06_03155"
     CDS             305..718
                     /locus_tag="VLL06_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSS29994.1"
                     /translation="MYTQYINMHSEFVISQSDGRPMYLQIMEQIRQRIAVGDWAKGQE
                     IPSIRQLAVTLRVSVITVKRAYLELEREGIIVTQQGKGSIVAPNSRLGTKLYDQELTK
                     HIDRIAQLGELLGLTPQELASRVREAADRLTKEYS"
     gene            715..1611
                     /locus_tag="VLL06_03160"
     CDS             715..1611
                     /locus_tag="VLL06_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSS29995.1"
                     /translation="MNDLSVHFERVSKIYPHFTLDDISLELPTGTIMGLIGANGAGKS
                     TTIRILMGLVHQDQGQVHVLGRAMPEEQAAAKFDIGFVSEDMRLYGSATLVWHMKFVR
                     SIYAQWDQPYAEMLLRRFDLKPQQKIKGLSHGQRVKAALLLALARRPRLLVLDEPTTG
                     LDPVARQEVLGELMAVLADEDRTILFSSHNTLDVEQISDQITFIDRGRIIASDNKEIF
                     LDRWRRLRLVLPPDADLPSLPGVVQVGGSGRLPVLVTNRFDPAMVSVCNDAGATVQAV
                     DPMTLEEIFVANVHSRREKQTL"
     gene            1620..>2065
                     /locus_tag="VLL06_03165"
     CDS             1620..>2065
                     /locus_tag="VLL06_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HSS29996.1"
                     /translation="MMKRLILKDWYLQRWAILASLAGVLVTLGIIATGGKVAFLLGLI
                     LLIMVIISIGAQMPVATIVTERKEQTLPFVMTLPISYREYTTSKIWGNLLIFLVPWMT
                     MLLGSIALLMYDPKSHGLIPYVAIMATEILMSTCLVLCVALITESQG"
BASE COUNT          460 a          581 c          551 g          473 t
ORIGIN      
        1 gtaagggact ccccgattct tttcggcgac tcttttctct tgtcccccac aagctctttg
       61 gatcccatca gtgattgcaa tcacatagtt ttctcagttc ttgacgggtg cttcaagctc
      121 gaccgccgct cgtccgccat cctctggaac aaaggtggct ttctgaaaga aaagtggccg
      181 attcgcacac tccgaatccg cattactcac caccctcgtc agtagtgtgc tatatagcgt
      241 aatcaaaaac tccatgcgca aaaatcatcg tcctgaaatt cactcttgac tgcggagaat
      301 gactgtgtat actcagtata taaacatgca cagcgagttt gtgatctctc aatccgacgg
      361 acggcccatg tatctgcaaa tcatggagca gatcaggcag aggatcgcgg tgggggattg
      421 ggcgaagggg caagagattc cctccattcg ccagcttgct gtaacccttc gggtcagcgt
      481 gatcacagtg aagcgcgctt acttggaact ggaacgtgag ggcatcatcg tgacccaaca
      541 gggaaaaggg tcgatcgtcg cgcccaattc gaggctgggc acaaagctct acgatcagga
      601 attaaccaag cacatcgacc gaattgccca actgggagaa ctgctgggcc tcacgcctca
      661 agaacttgct tcccgggtgc gcgaggctgc ggaccggttg accaaggagt actcatgaat
      721 gacctctccg ttcatttcga aagagtgagc aagatctacc ctcacttcac cctcgacgac
      781 attagcctgg aattgcccac gggcacgatc atgggcctga tcggcgcaaa cggcgccggg
      841 aagtccacga ccattcgcat cctgatggga ctggttcacc aggatcaggg tcaggtgcat
      901 gtcctgggcc gagccatgcc cgaggaacaa gcggcagcca agtttgacat tggattcgta
      961 tcggaagaca tgcgtctgta tggatcggcg acgctggtct ggcacatgaa atttgttcgg
     1021 tcgatctatg cacagtggga tcaaccctac gccgaaatgc tgttgcggcg ctttgacctc
     1081 aagccccagc aaaagatcaa aggtctgtct catggacaac gcgtcaaggc ggcattattg
     1141 ctggccctgg cacggcggcc gcgccttctg gtgctcgacg aacccaccac cggactcgat
     1201 cccgtagcgc gtcaagaggt gctgggagaa ctgatggcag tgcttgccga tgaggatcga
     1261 acaattcttt tctcgtcgca caacactctc gacgtcgagc agatttccga tcagatcacg
     1321 ttcatcgatc gcgggcgaat catcgcctcc gacaacaaag agatatttct ggaccgttgg
     1381 cgccgcttgc gcctcgtgct cccgccggac gcagatttgc ccagtcttcc gggagtggtt
     1441 caggtcggcg gaagcggacg gcttcccgta ctggttacca accgtttcga tcctgcgatg
     1501 gtctcggtct gcaatgatgc gggtgccacc gttcaagccg tcgacccaat gacgctggaa
     1561 gaaatttttg ttgcgaatgt gcacagcaga agggaaaaac aaaccctatg aattacgcga
     1621 tgatgaagcg tctcattttg aaggactggt acttgcaacg ttgggcgatt ctcgcctctc
     1681 tcgccggagt tttggttacc cttggcatca ttgcaacggg aggaaaggtc gcgttccttc
     1741 ttggcctgat ccttcttatc atggtgatca tctccatcgg tgcccagatg cctgtggcca
     1801 cgatcgtgac agagcggaaa gagcagacgc tcccctttgt gatgaccctg cccatctcgt
     1861 accgggaata cacgacgtca aagatttggg gcaacctcct catcttcctg gttccctgga
     1921 tgaccatgct gctgggcagc atcgcactgc ttatgtacga tccgaaatcg cacgggttga
     1981 tcccctatgt cgccatcatg gcgactgaaa tcctgatgag cacttgcctc gtcctctgcg
     2041 tggctctgat caccgagtct caggg
//
LOCUS       DATIPF010000123         3145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_728086, whole genome shotgun sequence.
ACCESSION   DATIPF010000123 DATIPF010000000
VERSION     DATIPF010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728086"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VLL06_03170"
     CDS             complement(<1..414)
                     /locus_tag="VLL06_03170"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HSS29997.1"
                     /translation="MNIQVTSVSKKTERLSEAAASIFVISREDIRQSGYNSIPEVLRL
                     APNLQVARVDSSQYAITARGFNSTTANKLLVLIDGRTVYTPLFSGVFWDAQDTLLEDI
                     ERIEVISGPGGTLWGSNAVNGVINIITRRSQDTKGG"
     gene            complement(1178..1801)
                     /locus_tag="VLL06_03175"
     CDS             complement(1178..1801)
                     /locus_tag="VLL06_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS29998.1"
                     /translation="MIWTSEKPTKPGWYWYRSQQDRVQIIELIEWNKELRTIGVSPGM
                     YPHQETHRVPDGPGAEWAGPMEPPRDVQCVESAYSSAKDRQVPPTILLVDDEEMIRQV
                     AETLLTRHGYTVLAAEDGNACMKILQEYPGSIDLLITDVFMPGMNGRKVADHLHTLRK
                     ETKILFMSGRSNEEIFGHDGLYPEIVFLPKPFTPETLLRKVSETLSP"
     gene            complement(1949..2590)
                     /locus_tag="VLL06_03180"
     CDS             complement(1949..2590)
                     /locus_tag="VLL06_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS29999.1"
                     /translation="MAILIVDDSRDEQELLTNRLQTAGYELLLVADSAEAAFGILGQV
                     EAGQRTREVDLILMDIMLPGLDGLDACRRITTTERLQDIPIIVITAKTGEQDLRAAFA
                     AGAMDYIRKPVNSVELVARVSSALTLRAERIMRKAREQELLTRTQELEHALREVKVLR
                     GFIPICAHCKRVRTDTGDWQRLEDYVQKHSEAEFSHGICQPCMREMYPEASID"
     gene            complement(2614..2982)
                     /locus_tag="VLL06_03185"
     CDS             complement(2614..2982)
                     /locus_tag="VLL06_03185"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS30000.1"
                     /translation="MDNENSPSSQPVSPSLREPQEARKFRRILVDLPCLFSTEGPREW
                     SGTTVNLSREGCAIRSTTPVQKNQDLCVLLFPSANQTPIEVEVAPVRWSANDQCGVEF
                     ITLTPRDANRLQDLLTIIGA"
BASE COUNT          689 a          842 c          872 g          742 t
ORIGIN      
        1 cccgcccttg gtgtcctgtg accgtcgcgt aatgatgttg atgacaccat taaccgcgtt
       61 cgagccccac aacgtgccgc ccggtccgct gatgacttca atccgctcga tgtcttcaag
      121 caacgtgtct tgcgcgtccc agaacacccc ggaaaacagc ggcgtataga cggtacgtcc
      181 gtcaatgagt acaagcaatt tgttggcggt ggtactgttg aagcctcgtg ccgtgatggc
      241 atattgactg gaatccaccc gcgcaacctg gaggttcggt gccagtcgca ggacctctgg
      301 aatgctgttg taaccggact ggcggatgtc ttccctcgag atgacgaaga tcgatgcagc
      361 cgcctcgctg agtcgttcag tcttcttgga gaccgaggtg acctgaatat tcatcagctc
      421 ttcgagactc aactctgtaa gctcgctagg ggcttctgcg gcgtgggctg gtgcgctagc
      481 acagataatg agggcagcca cccccagcaa gcaggcattt atgaagcgtg cacttagata
      541 tgtgttgacg gtttgcaaga ctatgcctct ggtgccgatt cgaaaagtgt gcggaagtca
      601 acctgtacaa gaatggctga tgactctgtt accgaggcgt ccatccagtg tccgttcatt
      661 ggagctggcg gcgaactggg cagcatgctc cggtctcaaa aattcgtgag aacgaccgcg
      721 aaacataagg ttaagaatac tctttgcctg aggcatcgca atctttttcc cattacgtac
      781 tgagataagc cggatggata agaggccgaa ctatggcctt cagagctacg gccgtcagct
      841 ctggggatgg cagtcagcga ccctgagggt cgggccactg gtgtccacga ggttaacgga
      901 gcgagtcgag cggcggaaag cggggatcat aatgcgtggt gccctgacgc ataaggaagg
      961 gtgggcgaag aggtccacag gaagcggcga ctcatgattc gaacgcgaca agctaagtgg
     1021 tccactaagg gataacgggt acgtgtggat ccatggcgtg agtgcgcttg aggaccaaga
     1081 gagccttcag atcgagcatg ttgagggtca ggatgaccag gtctgggtga tcctctcgaa
     1141 tcatttcgac gccgttgtag cttcggcctg cctagtatta tgggctcagt gtctccgata
     1201 ccttacgaag gagagtttcc ggggtgaacg gtttgggaag aaagacgatc tctggatata
     1261 atccgtcatg accgaagatc tcctcgttcg aacggcccga cataaaaagt attttcgtct
     1321 ctttccgcaa tgtgtgcaga tggtcggcga ccttccgccc attcatgccc ggcatgaaga
     1381 catcggttat cagtaagtcg attgacccgg gatactcctg cagaattttc atacaagcgt
     1441 ttccgtcctc ggctgctaag acggtatagc catgcctcgt aagcaacgtc tcggccactt
     1501 gccggatcat ttcttcatcg tccaccagca gaatagttgg tggcacttgc cggtctttag
     1561 cggaggaata ggctgattcg acgcactgaa cgtctcgggg cggttccatc ggtccggccc
     1621 attcagcccc aggcccatca ggcactcgat gtgtctcctg gtgcgggtac atgcccggtg
     1681 atacgccgat tgttcgtagt tccttattcc attcgatgag ttcgataatc tgcacccgat
     1741 cttgttgaga tctataccag taccaccccg gtttggtcgg cttctcactc gtccagatca
     1801 ctctgccacc tcttccccta acgctggtgc agattacccc tagaacatca tttgttccat
     1861 gagctcgctg atcatccgcc ccagacggtc tgccgcggtg atccgaaaga aggccaaccc
     1921 gaacgtatgc ccgcccaccc tgtgcgtctc agtcgatgga ggcttctgga tacatctctc
     1981 tcatacaggg ctggcagatt ccatggctaa actcggcctc tgaatgtttt tgaacgtagt
     2041 cctcgagtcg ttgccaatcc ccggtatctg tccgtacccg tttgcagtgg gcacagatcg
     2101 gaatgaaccc gcgcaacacc ttaacttctc gtagcgcgtg ttcgagttcc tgtgtgcgcg
     2161 tgagtaattc ctgctctctg gccttgcgca taatccgttc tgctcgtaag gtgagcgccg
     2221 aggagacgcg agccacgagt tccactgaat tgaccggttt ccggatatag tccatcgccc
     2281 ctgccgcaaa tgctgcacgg agatcctgct caccggtttt cgcggttatg acgatgatgg
     2341 ggatatcctg gagcctctca gtcgtcgtaa tccggcgaca cgcgtccaat ccatccaggc
     2401 caggtagcat gatatccatg aggatgagat caacctctcg cgtcctctgc ccagcctcca
     2461 cttgtcccaa aatcccaaaa gcggcctcag cagaatcggc aacgagcagc aactcgtagc
     2521 cggcagtctg caacctgttc gtgagcaatt cttgctcgtc tcgtgaatca tctactatta
     2581 aaatggccat actgtgtcct ctacgcggac gcgctaggca ccaatgatcg tgagaagatc
     2641 ctgtaatcgg ttcgcatcgc gtggtgtcag cgtaataaat tccacgccac attggtcatt
     2701 cgcggaccat cgcacgggcg ccacctcgac ctcgatggga gtttggttcg cggaagggaa
     2761 tagcagcaca cagagatctt ggttcttttg gaccggtgtc gtactgcgga tggcgcaccc
     2821 ttcgcgggac aggttgacgg tcgtcccact ccattcacga ggaccctccg tcgaaaataa
     2881 acagggcaaa tcaaccagaa tacgtcggaa ttttctggcc tcctggggtt ccctgagaga
     2941 cggagagacg ggttgagaag atggagagtt ctcattatcc atgaatataa atgtactgct
     3001 tcctattgaa acgtcaaaat gatttcgcgt tatgtcccgt gtagtgcacg gtataggtgg
     3061 ggagaggcga agcgagaatg gcctgaaggc tttggaggga gcgatgtttg tgccaatcag
     3121 aggcaaagat tgatgggggt gaagt
//
LOCUS       DATIPF010000124         6663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1526695, whole genome shotgun sequence.
ACCESSION   DATIPF010000124 DATIPF010000000
VERSION     DATIPF010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526695"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /locus_tag="VLL06_03190"
     CDS             <1..1592
                     /locus_tag="VLL06_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSS30001.1"
                     /translation="LPAKTRAKNGRIRVMGFPPIGIMSLSSVLKQAGHECVMFDQANP
                     DTPNETIIEEINRQQPALVGLSFLSTTSYPYAKILARQIRAANSTVKLAFGGVFASLN
                     AALVKLQCPEVNFVCRGDGEQLLLDLLERIDDPAGVAGLTWAKDGQVVQNSGRPMERH
                     LDQWPFPDREGLPLDFIESMPLDVPAILSMERFTTMQTSRGCPWPCVFCDIPIFNEGK
                     WRARTAQHVVSELKYLEECGYGSVYFVDDHFLLQPKRIEAICKGVMDEKLTIQWGIEG
                     RVDSVAQHLFPAMAKAHCRTVMFGIESGSQKILDRLKKEQTLEEVATAVKNAKQAGIE
                     IVHGFFTVGNPDETVEDMNATFDLASKLPLDTFGFNRLCVYRGTPLWQEYVKRGLVSE
                     TSDWYKYFKCSAIDPTCLPGEVINEVRRRGLKRLLIYKLLHFPLQTYRLLRRFFRFMP
                     ARDVVYLIIKPFLGKHTVATKAEVLSRAVEHGEMKDAAAQLTQMSDELLNNVMEESRL
                     ERLRIQQEAESSRELPMVHTR"
     gene            complement(1822..1947)
                     /locus_tag="VLL06_03195"
     CDS             complement(1822..1947)
                     /locus_tag="VLL06_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30002.1"
                     /translation="MSVMCSLSVCKAQAGMCGHEKVMLSIALLAAFGVGAYFLIS"
     gene            complement(2123..3325)
                     /locus_tag="VLL06_03200"
     CDS             complement(2123..3325)
                     /locus_tag="VLL06_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30003.1"
                     /translation="MGSTPFIPLSLSDYDISPEQGFLPQDPCENLPDCTTLNHLGHEL
                     PKLLSARTVRKYIDGQRQLLPSIPPTWRSEDYRTAMRILSFAGHAYVWEVPDRPVATL
                     PPQLAQPWYEVAQRLGRPPVLSYASYALDNWRRLDRTQPIQLDNMVLLQNFLGGLDEE
                     WFVVVHIQIEREAGSGLAGLLRAMNGAAEDKKDEVILGLQSLAWAQTAMRNTLLRMKE
                     RCDPYVYYNRVRPYIHAWKNSPTLPNGLIYENIEAYAGQPQQFRGETGAQSSIVPCLD
                     AGLGIAHAPDPLTVYLQEMREYMPPRHRAFLESLEAAIDDRDRPLLSGYVRDRIFHSP
                     DLWTAYCACVDLLAQFREIHIGYADSYIHRQHQAHASNPTAVGTGGTPFMTYLQKHLD
                     ETKQAIVS"
     gene            3488..4633
                     /locus_tag="VLL06_03205"
     CDS             3488..4633
                     /locus_tag="VLL06_03205"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HSS30004.1"
                     /translation="MLKKPTNCAWMGVKQQLAGHGKTLSACWNLTCLHVWEIPGVQHT
                     GCSKRPFSKAAGEREPEAYHFSPFHPKLPRQLVLQMGYAEDSGELRSKLEEKRVSARR
                     GWAGKKGDFFSILIAGWLIVLYLLAGAGCAAGHSRHDRLAWEQSRLIDLTHSFGADTI
                     VWPTEPDFRLVLQQAGDTSGGYYYASNRLEMAEHGGTHIDAPIHFSKGGQTLDQVPIE
                     RLIGAGVRIDVAAQCAHDRDYLVTIQDFEQWEAEHGRIPIGAIVLVDTGFARYWPSRQ
                     QYLGTELRGSEGVRALHFPGLHPTAANWLVEQRQVKAVGIDTASIDYGQSTTFETHVA
                     LLSRNVLVLENLGNLRDLPDRGFDVIALPMKIAGGTGGPLRVIAVLP"
     gene            5097..5444
                     /locus_tag="VLL06_03210"
     CDS             5097..5444
                     /locus_tag="VLL06_03210"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS30005.1"
                     /translation="MPNGPRDGKTENRQYPRVAIQFRTHFSSKNRMVAGDGELKDLSP
                     GGCRVTSSVLAQVGTELEFCIFPADEDNPFIIDAATVRWARDQEFGLAFTQVRPSVQQ
                     QLVQLCRRRASLT"
     gene            5883..>6663
                     /locus_tag="VLL06_03215"
     CDS             5883..>6663
                     /locus_tag="VLL06_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30006.1"
                     /translation="MGKVGMKIKESQYRGFVLEPAAVNQAKVDDYTGALETVTKIPNS
                     DRAVLALADIAFIQTTKGHKQQGARTFRQAHARAAVLKDRNHKALVLGKIAELEGKSH
                     DANASKTLAEALQIVTGLPLDDKPDVLYRLGFYQFGAGQVEAVETLKQASQALSLISD
                     NRRKWMTAFQLAPLQIQVGDKQGALVTARLCVDEQDFYCRSYLLSSVAVQQAEKGMIS
                     EALETLSEVPNETEKQRALGAIVKAHLKNGDLQEASRLTETI"
BASE COUNT         1529 a         1743 c         1883 g         1508 t
ORIGIN      
        1 cgctcccagc aaaaacacgt gcgaaaaacg ggcgtatccg ggtgatgggt ttcccgccta
       61 tcggcatcat gtccctctcg tctgtgctta aacaggccgg ccacgaatgt gtgatgttcg
      121 atcaggcgaa tcccgacacg ccgaacgaga cgattatcga ggaaatcaat cggcaacaac
      181 cggctctggt cggccttagc tttctcagta cgaccagcta tccctatgcg aagatcttgg
      241 cccgccagat tcgcgccgcc aactcaacgg tcaaactggc gttcggcggt gtttttgcaa
      301 gtttgaatgc cgcgcttgtc aaactgcagt gccctgaagt gaatttcgtc tgccgtggag
      361 atggcgagca gttacttttg gacctcctcg aacgaattga tgacccagcc ggtgttgccg
      421 ggctgacctg ggccaaggat ggccaggtgg tgcagaattc cggtcggccg atggaacgcc
      481 atctcgatca gtggcccttc cccgatcgcg aaggtctccc gctcgatttc attgaatcga
      541 tgcccctcga cgtgcctgcg atactctcaa tggagcggtt cacgaccatg caaacctccc
      601 gaggctgccc atggccctgc gtgttctgcg acattccgat cttcaacgaa ggcaagtggc
      661 gtgcgcggac tgcccagcac gtggtaagcg aactcaagta cctcgaggaa tgcggctatg
      721 ggtccgtcta ttttgtcgac gatcatttcc tgctgcaacc taaacggatc gaggcgattt
      781 gcaaaggtgt gatggacgag aagctcacga tccaatgggg cattgaaggg cgcgtggact
      841 cggtcgcgca acatttgttt cctgctatgg cgaaggccca ttgccggacg gtcatgttcg
      901 ggattgaaag cgggagtcag aagattctcg atcgtctcaa gaaagagcag acgctggaag
      961 aggtggcgac tgcagtcaag aatgccaaac aggcaggcat cgagatcgtg catggcttct
     1021 ttacggtggg caatcccgat gaaacagtcg aagacatgaa cgccacgttc gacctggctt
     1081 ccaagttacc actcgatacg ttcgggttca atcggctctg tgtctatcga ggcaccccgc
     1141 tctggcaaga atatgtgaaa cgcgggttgg tcagcgaaac cagtgattgg tacaaatatt
     1201 ttaagtgttc tgcgatcgat ccgacctgct tgcccggtga agtgatcaat gaggttcgcc
     1261 gtcgagggct caagcggctg ttgatataca agctcctcca ctttcctctc cagacgtatc
     1321 ggctgctgag acggtttttc cggttcatgc cggcacgaga tgtcgtgtac ctcatcatca
     1381 agccgttctt aggcaagcat acagtcgcga cgaaggcaga agtgctatca agagcggtcg
     1441 aacatggcga gatgaaggat gctgcggcac aactgactca gatgtcggac gagctgctca
     1501 acaatgtcat ggaagagtcg cgcctggaac ggttacggat tcaacaggaa gcagagagtt
     1561 ctcgcgaact gccgatggtc cacacccgct agagcaggat gctgaaaaag cccgccagct
     1621 tcgttctcga ctcattgaaa tcctcaacgt acccctgagg gtacgcctcc ggtttcaatt
     1681 catctgcggc cttgctggac ggtctttttg agcatcctgc aagattagtc gcaatccaca
     1741 agatttgttt tgctgccaat cctattacac agctcatgcc caggtgagca tggtggacgt
     1801 gattcgtttt atcccatctc gtcaactgat caggaagtag gctccaacgc cgaaagctgc
     1861 gagcagggct atgctcagca tcaccttttc gtgcccacac atcccggctt gtgccttaca
     1921 gaccgacagt gagcacatga ccgacatcgg agcctccttt ggtgcagatt gagaatccta
     1981 ttcccaacga ttagatgtat taaacgatat gctgctcagc atgtctagtc aacgccgatc
     2041 cagttgaccc cctgccgttc acaagccatt tgctccagtc gatagtccag tagccatata
     2101 tgggcgagtc ggtcaacagg acctacgaaa caatcgcctg tttcgtttcg tccagatgtt
     2161 tctggagata ggtcatgaag ggtgtgccgc ctgtgcccac agctgtggga ttacttgcgt
     2221 gggcctgatg ctgtcggtga atgtagctgt cggcgtagcc gatgtgaatc tcgcgaaatt
     2281 gagcaaggag gtcgacgcag gcgcaatagg cagtccagag atctgggctg tggaatatcc
     2341 ggtcgcgaac atagccggag agcagggggc gatctcggtc atcgatagcg gcctctaggg
     2401 attccagaaa ggcacgatgg cgcgggggca tatactctcg catttcctgc agatagacgg
     2461 tcagggggtc aggcgcatgg gcgatcccga gcccggcgtc gagacagggg acaatagagc
     2521 tttgtgcgcc ggtctcacct cgaaactgtt gtggctgccc ggcgtaggct tcgatgttct
     2581 cgtagatgag cccgttcggt agggtagggc tgttcttcca cgcatggata tagggccgta
     2641 cacgattgta gtacacatag ggatcgcagc gctctttcat cctgagcagc gtgttgcgca
     2701 tcgccgtttg ggcccacgcc aaggattgaa gcccaaggat gacctcgtcc ttcttgtcct
     2761 cggcagcacc attcatggct cgcaggagtc cggccagtcc cgatcccgct tcccgctcga
     2821 tctggatgtg caccacgaca aaccattctt catccaaacc gccgagaaag ttttgcagca
     2881 acaccatgtt gtcgagctga atcggctgag tccggtcgag tcgccgccaa ttgtccagtg
     2941 cataggacgc ataggacagg acgggtggac ggccaagccg ttgcgccacc tcgtaccaag
     3001 gttgtgccaa ttgtggtggc aatgtcgcaa ccggccggtc cggtacttcc cagacatagg
     3061 catgtccggc gaacgagagg atccgcatcg cagtccgata gtcttcgctg cgccatgtcg
     3121 ggggaatgga agggagcagc tgccgttgcc cgtcgatgta cttccgtaca gtccgggcgc
     3181 tcagcaattt tggtaactca tgcccaagat ggttcagagt cgtgcagtcg ggcaggtttt
     3241 cacaagggtc ttgaggaagg aacccctgtt caggggagat gtcgtaatcg gagagcgaaa
     3301 gaggaatgaa aggggtcgat cccatagaag cctccggtac gcacaacgtc agcgaccgtt
     3361 tcactgtatc acacatcacg cgaatcctcg ccgaccgcgg aatggaaaat cagagtgccc
     3421 ctgtttgctt tcttgcccag ttgactgaga atatttgcca gatgtttcca caacgaagga
     3481 gcctgtcatg ttgaagaagc caacgaattg tgcatggatg ggggtgaagc aacagttagc
     3541 cggccacggt aaaactctat cggcatgctg gaatcttact tgtctgcacg tctgggagat
     3601 accaggagtt caacacacag gatgctcaaa aaggccgttc agcaaggctg caggcgagcg
     3661 agaaccggag gcgtaccatt tctcaccctt ccaccccaag ctgccaagac agcttgttct
     3721 ccaaatgggg tacgctgaag attctggcga gctgagatcg aagctcgagg aaaagcgcgt
     3781 ctcggcgcgc aggggttggg cgggtaagaa gggcgacttt ttcagcattc tgatagccgg
     3841 ctggctcatc gtcctctacc tgctggctgg tgcgggatgt gctgccggtc attcccggca
     3901 tgatcgtctt gcctgggagc agtcgcgtct catcgatctc actcattcct tcggggccga
     3961 cacgattgtc tggccgactg aaccggactt ccggctcgtt cttcagcagg cgggggatac
     4021 atcgggcgga tactattacg cgtcgaatcg actagagatg gccgaacacg ggggcaccca
     4081 catcgatgcc cccattcatt tttccaaggg cgggcagacg ctggatcagg ttccgatcga
     4141 acgtttaatt ggggccggcg tccgaatcga cgtggctgct caatgtgccc acgatcgtga
     4201 ttacctcgtc accatccaag attttgagca atgggaagca gaacatgggc gaatcccgat
     4261 tggcgcgatt gtgctggtcg acacaggctt tgcccggtac tggccgtcgc ggcaacagta
     4321 cctgggaaca gagctgagag gatcggaggg tgtacgagcg ctccatttcc ctggcttgca
     4381 tcctacggct gcgaactggc ttgtggagca gcggcaggtc aaagccgtcg gaattgatac
     4441 cgcctcgatc gactatggac aatccaccac atttgagacg catgtcgcgc ttctctccag
     4501 aaacgtgctg gtgctcgaaa atcttggcaa tctacgagac cttccggatc ggggattcga
     4561 cgtgatcgcc ctgccgatga aaattgccgg cgggactggc gggccgctgc gcgtcattgc
     4621 ggtgctccct tagcttggag caaagcggag cgtgggaacc aagaggcggc cgcgtttacg
     4681 gtcaagttgc tcccggcgtt tcctactgcc taggaaggca actcggaatg tcctgggcgg
     4741 gactgtttca ccatccaata ttcagaactc aggaacggag tctctatttt ctgccacttt
     4801 tcgcctgtct tttcgtaagc ggcaaaacga gaccatttgg gccagtatcc atgctgaaaa
     4861 gtatctctga acatcagcgt gtactgtatg tgatccaccg ttgtatctga aaggatgctc
     4921 ccataaccat ggtatgctag tattctgagt tcctactatt tgaaaatcca caataggagg
     4981 cagtttcata ccctggcacc aggggtgcat tagtggaatg agtgcgtatg atgggatagg
     5041 ataggcctta ggatccaata gcagttgttg attggcccga gtgaggagat cgagagatgc
     5101 ctaacggtcc aagagacggc aaaacagaga accggcagta cccccgtgtg gccatccaat
     5161 ttcgaaccca cttctcatcg aagaatcgga tggtggccgg tgacggcgag ttgaaggact
     5221 tgtcgcctgg aggctgtcgc gtcacaagtt ctgtgttggc tcaagtcgga accgagctgg
     5281 aattctgcat ctttcccgcc gatgaagaca acccattcat tatcgatgct gcaacggttc
     5341 gctgggctcg tgaccaagag ttcggcctcg cgtttaccca ggtccgtcca tctgtgcaac
     5401 aacagttagt tcagctctgc cgaagacgtg catccctcac gtgagagata gctcgactct
     5461 cctaatggtc ttcatttcaa agtcagttac ttcttgcgcg atgtacttat ttcaaaaacc
     5521 tgatcgaacc ggtaagagcg accgcctgaa taatcaagag gttttgattt tgaggcagta
     5581 gggtcaaaaa caaatgtatt tgggaaattt cgagtcacgc caggacgtta ttgtctcccc
     5641 acagatgaaa cgcatttcct aggtgggttc ggatacccat gctagtctca gcgtctccat
     5701 gggacaacta ataagaggta ggttgtttca atgagggacg gatgaatcct ttcaaggcgc
     5761 tagtgtttgc attcacattg gccctaccat gtcttcctgc tctcagcagt gccgaagagc
     5821 atggtacccc tcccagtaac aagcaggatg atttcgccct tgccagaaag gttctcaggg
     5881 acatgggaaa ggttggcatg aagattaagg agtcgcagta cagagggttc gtccttgaac
     5941 cagctgcggt caatcaggca aaggtcgatg attatacggg tgcactggag acagtaacca
     6001 agattcccaa ttcggatcga gccgtactcg ccttggcgga tatcgcattc atccaaacca
     6061 ccaaggggca caagcaacag ggtgcaagaa cttttcgaca ggctcatgca cgggctgccg
     6121 ttttgaagga ccggaatcac aaggctcttg tgttgggtaa gattgctgag cttgaaggca
     6181 aatcacatga tgcaaatgcc tcaaaaactt tggcggaggc gcttcaaatc gtcactggac
     6241 tccctttgga tgacaaaccc gacgtattat accgtcttgg gttctatcaa ttcggcgctg
     6301 ggcaggtaga ggcagttgag acattgaagc aggccagtca agcgctctct ctgatctctg
     6361 acaaccgtag gaagtggatg acggccttcc agctggcccc tctccaaatt caagtgggag
     6421 ataagcaagg tgcgctcgtc acggctcgtt tgtgtgtcga tgaacaagat ttctactgta
     6481 gaagttatct gttgagtagc gttgcagttc aacaagccga gaagggaatg atctcagaag
     6541 cattggagac cttgtccgaa gttccgaatg agacggagaa gcagcgagcg ctaggcgcca
     6601 tcgtcaaggc gcacctcaag aacggggatc tccaggaagc cagtcgtcta acggaaacga
     6661 tcc
//
LOCUS       DATIPF010000125         3888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_23659, whole genome shotgun sequence.
ACCESSION   DATIPF010000125 DATIPF010000000
VERSION     DATIPF010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23659"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VLL06_03220"
     CDS             complement(<1..1033)
                     /locus_tag="VLL06_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSS30007.1"
                     /translation="MKLPIFLDNHSTTPMDPRVLETMLPYFVEKFGNAASRNHAFGWA
                     AEEAVESSRKQIAKLIKADAKEIVFTSGATESDNLAIKGVLEMYKEKGDHIITASTEH
                     RAVLDTVKSLEGKGKATATYLSVDKFGMVSPEDVRNAITEKTILISIMLANNEIGTIN
                     PVKEIGKIAKEKGILFHCDATQGVGKIPVDVQDMGIDLMSFASHKIYGPKGVGALYVR
                     KKNPRVRIAAQMDGGGHERGMRSGTLPVPLIVGFGKACELCEQEMAKESTRLITMRNR
                     LESSIMTALEESYLNGHPTNRLPGNLNISFAYVEGESLLMGMKDIALSSGSACTSATL
                     EPSYVLRALG"
     gene            complement(1030..1503)
                     /locus_tag="VLL06_03225"
     CDS             complement(1030..1503)
                     /locus_tag="VLL06_03225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSS30008.1"
                     /translation="MLKISKKADYALMALQHIAAVQFGDVTPGRVVNTKEIAEEYNIP
                     LELLAKVLQTLAKNALIESHNGPKGGYVLARRAHQITIAQILESIEGPLGITDCSHEK
                     DGEICMQRENCHIRTPLLKVQDSIAQLLNNMTLQDMMGGTPLITIQSPTAQGVER"
     gene            1639..2055
                     /locus_tag="VLL06_03230"
     CDS             1639..2055
                     /locus_tag="VLL06_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HSS30009.1"
                     /translation="MGGTNPYIEKAEYELPQRAYTVTFMAPDGGVTKIEVDPAKIPYG
                     PTGLPGSLLDVAMGNGVALEHVCGGVCACSTCHIIVKQGLESCNEGTDDEFDQLEEAP
                     MTTLQSRLGCQCVPNGTKDIVVEIPAVNKNLVREGH"
     gene            complement(2052..2756)
                     /locus_tag="VLL06_03235"
     CDS             complement(2052..2756)
                     /locus_tag="VLL06_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSS30010.1"
                     /translation="MAELTQPATVLSITDLTPNVRQLVVLPKIQRVLFQPGQWVSLQL
                     PVGTKPPLNRAYSMAEPPSPDGQLTLVFDRVKDGIGSNYLYGLTPGDELLLSGPYGNF
                     QLPLSLDQDLLFISRYTGLVPIRCMLKGRAASGALPQFILIAVGPTEDEWLYHDELLA
                     LAVRHPSFRYLPVAVNGMDQEVVEATVKLLRPLVTGRPRVTILLSGVKEFVRPLRAYL
                     VETGYGRKEIKTETYN"
     gene            2890..3873
                     /gene="gltX"
                     /locus_tag="VLL06_03240"
     CDS             2890..3873
                     /gene="gltX"
                     /locus_tag="VLL06_03240"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HSS30011.1"
                     /translation="MSQVRVRFAPSPTGVLHIGGVRTALFNWLFARQQNGVFVLRIED
                     TDQSRSTDESIQAIVEGMKWVGLDWDEGPFRQTERMDLYRSHAMQLLEKGQAYWCSCK
                     AEELDARRKEAESKGLSPRYDGRCRNRGLTNQTGDAALRFKAPQEGETVVDDLIKGKI
                     LFDNSVQDDLIILRSNGYPTYNFSVVVDDAMMNITHVVRGDDHLTNTPRQVPIFQALG
                     FSVPLFGHVPMILGSDRTRLSKRHGATSIMAYKDMGYLPDTMVNYLVRLGWSHGDQEI
                     FTREELIEKFTWKNVQTSAAVFNPEKLLWVNAEYIKITPPAKVAQALVPFL"
BASE COUNT          878 a         1001 c         1071 g          938 t
ORIGIN      
        1 cccccaacgc gcggagtacg taagagggct ccaatgtggc cgaggtacag gccgaaccgg
       61 atgagagggc gatgtccttc atgcccatca gtaacgactc gccttccaca taggcaaagg
      121 agatattaag attgccgggg agtctgttgg tcggatgtcc gttcagataa ctttcttcga
      181 gggccgtcat gatactggac tccagccgat tgcgcatcgt aattaaccgt gtggattctt
      241 tggccatctc ctgctcgcag agttcgcaag ccttgccgaa ccccacgatt aagggcaccg
      301 ggagcgtccc ggagcgcatg cctcgctcgt gtccgccgcc atccatttgc gccgcgatgc
      361 gaacccgagg attcttcttt ctcacgtaga gtgccccgac ccccttgggt ccgtatatct
      421 tatggctcgc gaaggacatg agatcgatcc ccatatcctg cacatcgacc gggatcttgc
      481 cgacgccctg tgtcgcatcg cagtgaaaaa ggatgccctt ttccttcgcg atcttaccga
      541 tctccttgac agggttgatc gtgccgattt cattgtttgc caacataata gagatgagga
      601 tcgtcttttc ggtaatggca ttccgtacat cctcaggact gaccatcccg aacttatcga
      661 cggagagata tgtggctgtc gctttgccct tgccttccaa cgatttgacg gtgtcgagta
      721 ccgcccgatg ttcagtggac gccgtaataa tatggtcgcc cttctcctta tacatttcca
      781 gcacaccttt gatcgcgaga ttatccgatt cagtggcgcc gcttgtgaag acgatctctt
      841 tggcatcggc cttgatcagc tttgcgatct gcttccggga actttcgaca gcttcctccg
      901 ccgcccaccc aaaggcgtgg ttacggctgg ccgcattccc gaatttctcg acgaagtagg
      961 ggagcatggt ttccaatacg cgtggatcca tcggtgtcgt ggaatggttg tccaagaaaa
     1021 tcggcagttt catcgttcaa ctccttgtgc cgtgggggac tggatcgtaa tgaggggtgt
     1081 accacccatc atgtcttgca gggtcatgtt attgagcaat tgggcgatac tatcctggac
     1141 tttcaagagc ggtgtacgga tatggcaatt ttcccgctgc atgcagatct ctccatcctt
     1201 ctcatgtgaa caatcggtga tacctaatgg gccctcaata ctttcgagaa tttgtgcgat
     1261 cgtaatctgg tgtgcgcgtc gggccagtac gtagccgcct ttgggaccat tatgactctc
     1321 gatcagtgcg ttcttggcaa gggtctggag tactttggct aacagctcga gggggatgtt
     1381 atattcctct gcgatctcct tcgtattcac cacgcgccca ggtgtcacgt cgccgaactg
     1441 gacggcagcg atgtgctgaa gggccatgag ggcatagtcg gcttttttcg aaatcttcaa
     1501 cattgctatt gccgatctcc aagatcggag actataatga tctccgatca ataaggtcgg
     1561 actaaggata gcatggaggt ttctaagctg tcaacagagt gaaataaggt tggatgaggc
     1621 tgagaaagga acgggcaaat gggcgggacc aatccctata ttgaaaaagc tgaatatgag
     1681 cttcctcagc gagcctatac ggtcacgttt atggcccctg acggtggggt gacgaagatt
     1741 gaggtcgatc cagccaagat cccttatggc ccgacggggc tcccggggag tctgctggat
     1801 gtggccatgg gcaatggggt tgcattggag catgtgtgcg ggggtgtctg tgcctgttcg
     1861 acctgtcaca tcattgtgaa acagggactt gagagctgta atgaaggtac ggatgatgag
     1921 ttcgatcagc tggaagaagc accgatgacc acgttacagt cacggctcgg ctgtcaatgc
     1981 gtgccaaacg ggacgaaaga cattgttgtt gaaattccgg cggtcaacaa gaacctggtg
     2041 agagaaggac attaattata cgtttcggtt ttgatctcct ttcgaccgta tccagtctcc
     2101 acgagatagg cccgcaacgg tcgcacgaat tctttgaccc cgctgagcag tattgtcact
     2161 ctgggtctgc cggtgacaag cggtcgcaat agtttcacgg tcgcctccac gacctcttgg
     2221 tccattccat ttaccgccac cggcaaatat cggaacgatg ggtgtcgcac ggctaaggcg
     2281 agtagctcat cgtggtagag ccattcatcc tcagtcggac cgaccgcaat caggatgaat
     2341 tgtggcagtg cgcccgatgc cgcccgccct ttcaacatac aacgaatcgg gaccagacca
     2401 gtatagcgac taataaagag cagatcttga tcgagtgaga ggggcaattg gaaatttccg
     2461 tagggaccag acagtagcag ctcatcgccg ggcgtgagcc catagagata attagaaccg
     2521 attccgtcct ttacgcgatc gaaaaccaga gtgagctgcc catcaggaga gggaggttct
     2581 gccattgaat aagcacgatt aagaggtggt ttcgtgccca ctggtaactg taaggagacc
     2641 cactgacctg gttggaataa aactctctga atcttcggaa ggacaaccaa ttggcgaacg
     2701 ttgggtgtga gatccgtgat tgagaggacc gttgccggtt gtgtgagttc cgccatcacg
     2761 atctcccact tgctctcata ccagaagccc gctccagatg aaagggtgcg cgacaaccga
     2821 ttctgtacaa cacttttacg cccgtgttat agataccgac cttttctacc atgacgcagg
     2881 gtcattttta tgagtcaagt cagggtcaga ttcgctccta gcccgactgg tgttctccac
     2941 atcggtgggg ttcgcacggc actgttcaat tggctctttg cgcgccagca gaatggcgtg
     3001 tttgtcctcc gtatcgaaga cacggaccag agccgctcga ccgatgaatc gatccaagcc
     3061 attgttgagg gaatgaagtg ggttgggctc gattgggacg agggcccctt ccgccagact
     3121 gagcgcatgg acctctatcg aagccacgca atgcagcttc ttgagaaggg gcaggcctat
     3181 tggtgctcct gcaaagccga agaacttgac gcgcggcgta aggaagctga atccaaggga
     3241 ctgtcgccac gatacgacgg ccgctgccgt aatcgtggct tgacgaatca gaccggcgac
     3301 gcggccctgc ggttcaaggc tccgcaagaa ggggagactg tggtcgatga cctcatcaag
     3361 gggaaaatcc tcttcgataa ttctgtgcag gatgatctga tcattctccg gtcgaacggc
     3421 tatccgacgt acaatttttc agtggtggtt gatgatgcga tgatgaacat tacccacgta
     3481 gtacgaggag acgatcacct gaccaataca ccgaggcagg ttccgatttt ccaagccctt
     3541 gggttttcag tccctctatt tggacacgtg ccgatgattc tgggctcgga cagaacccgc
     3601 ctttcgaagc gacatggggc cacctcgatc atggcctata aagacatggg ctatcttccg
     3661 gataccatgg tgaattatct cgttcgtctt gggtggtcac acggggacca ggaaattttt
     3721 acacgagagg agctgatcga gaagttcacc tggaagaatg ttcaaacgtc ggctgccgtg
     3781 ttcaatcccg agaaacttct ctgggtgaac gcggaataca ttaagattac cccgcctgcc
     3841 aaggttgcac aggcgcttgt gcctttcttg taagccgcgg gattacaa
//
LOCUS       DATIPF010000126         5180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1527398, whole genome shotgun sequence.
ACCESSION   DATIPF010000126 DATIPF010000000
VERSION     DATIPF010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527398"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..743
                     /locus_tag="VLL06_03245"
     CDS             66..743
                     /locus_tag="VLL06_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30012.1"
                     /translation="MTFHTKGLAVMAAAALVVSVASTALAIESFQERFEWGDLSKPTT
                     LQGRVIVLDHYDEAVWINISVFGGAAESGFFWQKIHPGKTLKFYADKGAWDELKKMAR
                     PHAGPAAAKEVPPSSTTDLIEFVVTEPEQNHRVISSVKKLPEVAGSMGKPLSISGLRL
                     KECAGKSEVEAGCAAAKQQLNTSPKSPDGADVLMQYDVLIPGGQPVAGLIPWTAKYNT
                     GAPAAAH"
     gene            complement(800..3466)
                     /gene="mutS"
                     /locus_tag="VLL06_03250"
     CDS             complement(800..3466)
                     /gene="mutS"
                     /locus_tag="VLL06_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HSS30013.1"
                     /translation="MSDADASPLMRQYREIKRGYPDAILLFRVGDFYEMFDADAQIAA
                     KLLSIALTSRDKSSTNPTPLCGVPYHAAQGYIAKLLKAGRTVALCEQVEDPKLAKGLV
                     RREVVRLYTPGTLVDTEFLSPGESHFLAAVAFLDAKGPSDKGQSIIGLACVDVSTGEF
                     WMTEFQGTQAEIQLMDELARLEPREVLHSDSKTNLGSCLSRLRGPRLCAQPSASFNPK
                     EATQLLQTQFAVHSLDGFGCRGLTAGIGAAGAVLQYFRETQPTASLAHIRRLHTRWSS
                     DAMHLDSATIRNLELVRPLGAGEHRSEQGQPTVLSVLDRTTTAMGSRLLRDWLVRPLL
                     SCGAIHARLDAVGEMKDRIQQRVSLRTTLRDVQDIARLSSRITLGVAGPRELLALKQS
                     VSALPELRSYLQSFHASLLTDVRESWDDCRDVQDAIEQAIHPNAPMSIRDGGLIRDGY
                     HSGVDELRKASTEGKGWIATLEGKERTRTGIDSLKVRYNQVFGYYIEITKTHLSRVPP
                     DYIRKQTLVNAERFMTPELKELEERVTGAEVKLLALEQELFEELRGRLAKEVARLQAM
                     AQTVALLDVLSGLAETAALHRYIKPLVDESSTIHIREGRHPVVEQLSSDLTFVPNDTT
                     LDCEGNRLVILTGPNMAGKSTFLRQVALIVLLAQIGSFVPATEARIGLVDRIFTRVGA
                     SDNLAAGQSTFMVEMIESAHILNSATSRSLILLDEIGRGTSTYDGLSIAWAIAEHIQD
                     RRHLGARTLFATHYHEMTQLEGLREGIKNYCVAVQERDGNVVFMRKIVPGGADRSYGI
                     HVAKLAGLPPEVITRAEEVLAQLERSDTTIEGISVSSDKEPSHASLPQPHPIIEEVKQ
                     IDLFSMTPLDALNRLADLQRRIGPTGQDTRDK"
     gene            complement(3459..3839)
                     /locus_tag="VLL06_03255"
     CDS             complement(3459..3839)
                     /locus_tag="VLL06_03255"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HSS30014.1"
                     /translation="MPALEQEDHSKVMIRVILVGILLLLSLAFFLQNQEQEVTLRYFF
                     GLFEASTPIYKPILMGFAVGLLVSGILLFPPWVRGRIELRRKTKALQDAEVDLERLRH
                     SLEKDTGQISPAHTGESHRDLHDE"
     gene            complement(3800..4396)
                     /gene="tsaE"
                     /locus_tag="VLL06_03260"
     CDS             complement(3800..4396)
                     /gene="tsaE"
                     /locus_tag="VLL06_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HSS30015.1"
                     /translation="MTRPAKAQQPAARRALTANLPWKLVSTSHPHTTRLGETIGRVLR
                     GGETIALYGPLGAGKTALVRGIAQGLGASPTAVSSPTFVVIREYDQGRLPLAHVDLYR
                     IRSPRELESTGLIEYFSGQTVTAIEWADKGLAALPPDRIEIALNHRAARSRTIQLSVT
                     GPKSHKVLAHARMQYSKTSRGSRGSSCRPLNRKTTAKS"
     gene            complement(4398..5174)
                     /locus_tag="VLL06_03265"
                     /pseudo
     CDS             complement(4398..5174)
                     /locus_tag="VLL06_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013425.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
BASE COUNT         1049 a         1414 c         1582 g         1135 t
ORIGIN      
        1 aggtgattaa gtaaccaacc ttgacaagca gtaatttttc ttgttctgtt aaggaggatg
       61 cgattatgac gtttcatacg aagggcttgg ctgtcatggc cgccgcagcg ctggtggttt
      121 ctgtggcctc caccgcactg gcaatcgaat cattccaaga gcgttttgaa tggggagact
      181 taagcaagcc gacgacattg cagggtcgcg tgattgtgct cgatcactat gatgaagcgg
      241 tctggatcaa catttctgtg ttcggagggg ctgctgagag cggtttcttc tggcagaaga
      301 ttcacccggg caagaccctc aagttttatg cagacaaggg agcgtgggac gagctgaaaa
      361 agatggcccg tccccatgcg ggaccggctg ctgccaagga agtgccgccg tccagcacaa
      421 ccgatttgat tgaatttgtc gtgactgagc cagaacagaa ccaccgtgtg atctcctcgg
      481 ttaagaagct tcccgaagtg gccggttcta tggggaagcc gctcagcatc tccggtcttc
      541 gattgaagga atgcgcaggg aagagcgaag ttgaggcagg ctgtgcagca gcgaagcaac
      601 agctcaatac ctcaccgaag tcaccggatg gggccgacgt gctcatgcag tacgatgtgt
      661 tgattccagg tggacagccc gtcgctggct tgattccctg gactgcgaag tacaacacgg
      721 gcgctccggc ggctgcgcac tagattgaat ccgtctctgt ttatccaggc ggcgtctccg
      781 aaaggggatg ccgcctgttt catttgtcgc gcgtgtcttg accggtcgga ccaatccgac
      841 gttgtaggtc agcgaggcga ttgagggcat cgagtggtgt catggagaag aggtcgattt
      901 gttttacttc ctcgatgatc ggatgtggct ggggaagtga ggcatgtgat ggttccttgt
      961 ccgaagaaac ggatatgccc tcgatggtgg tatccgaccg ttccaattgg gccaggactt
     1021 cctctgcccg agtaataacc tctggaggaa gtcccgccag tttggcaaca tgaataccgt
     1081 agctgcggtc tgcgccgcct ggaacaattt tacgcatgaa gaccacgttg ccatcccgtt
     1141 cctggaccgc cacgcaatag tttttgatcc cttcacgtaa cccttcaagc tgcgtcatct
     1201 cgtgatagtg agtggcaaac agcgtcctgg cgcctaagtg ccgacggtct tgaatatgtt
     1261 cagcaatagc ccaggcgatg ctcagtccat cgtaggtact ggtccctcgg ccgatctcat
     1321 caaggagaat caagctgcgg gaggtcgcac tattcaagat gtgagcggat tcgatcattt
     1381 ccaccatgaa ggtgctctgt ccggccgcca gattgtccga ggctcctact cgcgtgaaaa
     1441 tgcgatcgac cagcccgatg cgggcctcgg ttgccggcac gaaactcccg atttgcgcca
     1501 acaggacgat cagggcaacc tggcggagga aggtgctttt tcccgccatg ttgggtccgg
     1561 tgagtatcac cagtcgattg ccctcacagt cgagggtcgt gtcgttgggt acgaacgtca
     1621 gatcactgct gagctgctcg acgacagggt ggcggccttc ccgaatatgg atcgtgctgc
     1681 tctcgtcgac aaggggtttg atgtagcgat gcaatgcggc agtctcggcc aacccggaga
     1741 ggacgtcgag caacgcgacg gtctgggcca tcgcttgaag ccgagccacc tcctttgcca
     1801 agcggcctcg caattcttcg aacagctctt gttcaagcgc cagcagtttc acttcagcgc
     1861 ccgtgacacg ctcttctaac tctttcagtt ccggcgtcat gaatcgttcg gcattgacca
     1921 gtgtctgctt gcgaatgtaa tccggcggga cacgggacag gtgtgtcttg gtaatctcga
     1981 tgtagtaacc gaacacttga ttgtagcgaa cctttaatga atcgatcccc gttcgcgtcc
     2041 gttctttgcc ttccaacgtg gcgatccacc ccttgccctc cgtgcttgct ttgcgcagtt
     2101 catcgactcc ggaatggtag ccgtcccgaa tcaggccgcc atcacggatc gacatcggcg
     2161 catttggatg gatggcctgc tcaatcgcgt cttgcacatc tcggcagtcg tcccacgact
     2221 cgcggacgtc ggtcagcagc gaggcgtgga aggattggag gtaagaccgt agctctggca
     2281 gagcgctcac cgattgtttc aacgcgagga gttcgcgagg ccctgcgacg ccgagcgtga
     2341 tgcggctgct caggcgggcg atatcctgca catcacggag cgtagtccga agcgacacgc
     2401 gttgttgaat ccggtccttc atctcgccga ccgcatcgag tcgagcgtga atggcgccgc
     2461 agctgaggag cggtctgaca agccaatcgc gtaacaagcg actacccatg gccgtcgtgg
     2521 tgcggtctaa caccgacaag accgtcggct gaccctgctc cgatcgatgc tcaccggctc
     2581 ccaatggtcg tacgagttca agattacgaa ttgtcgcgct atcgagatgc atggcgtcac
     2641 tgctccagcg cgtgtgtaga cggcggatgt gtgcgaggga ggcggttggc tgggtttcac
     2701 gaaaatattg caggaccgct ccggcggctc cgatcccggc tgtcaagcct cgacagccga
     2761 atccgtcgag cgagtgcacc gcaaactgag tctggaggag ttgcgtggcc tctttgggat
     2821 tgaaggaggc cgagggttgt gcgcagaggc gtggtccccg caggcgactc aggcacgatc
     2881 cgagattcgt cttggaatcg gagtggagga cttctcgtgg ttccagccgg gccagttcat
     2941 ccatgagctg gatctctgcc tgtgtccctt ggaactctgt catccaaaac tctccagtgg
     3001 agacgtcgac acaggccaat ccaatgatgg attgtccctt atcagatggg cctttggcgt
     3061 cgaggaaggc gacagccgcc aggaaatgcg attctccggg agagaggaat tcagtatcga
     3121 ccagcgtgcc cggtgtatac aaacgcacga cttctcggcg cacaagaccc tttgcgagtt
     3181 tcggatcttc cacctgttcg cagagggcga cggtccgtcc ggccttgaga agttttgcaa
     3241 tataaccttg tgcggcatgg taaggcactc cgcagagggg agtcggattg gtgctggatt
     3301 tatcgcgaga ggtgagggca atagagagta gcttggcggc aatctgcgca tccgcatcaa
     3361 acatttcgta gaaatcaccg acgcgaaaca acaaaatggc gtcgggatag ccgcgcttga
     3421 tttcgcggta ttggcgcatc aatggtgagg cgtctgcgtc actcatcgtg cagatctcga
     3481 tgtgattcac cggtgtgtgc gggagatatc tggcctgtgt ctttttcgag tgagtggcgt
     3541 aatcgttcta agtccacctc agcgtcctgg agtgctttgg tttttctgcg gagttcgatc
     3601 cggccccgca cccatggagg aaagagaagg atgccggaca caagtagacc cactgcaaac
     3661 cccatcagaa tgggcttata aatcggcgtc gaggcctcaa agagcccaaa gaagtaccgg
     3721 agtgtgactt cctgctcctg gttctgaagg aaaaaagcca gggagagaag cagaaggatt
     3781 ccgactagaa tcaccctgat catgactttg ctgtggtctt cctgttcaag ggccggcatg
     3841 aggaccctcg agatcccctg ctggtcttgc tgtactgcat gcgcgcatgg gcgaggacct
     3901 tgtgagattt cggtcctgtc acgctcaact gaatggtccg gctccgtgcg gcacgatggt
     3961 tcagcgcaat ctcgatccgg tcaggaggta gtgcggcaag gcccttatcg gcccattcga
     4021 ttgccgtcac ggtttggccg gagaaatact cgatcaatcc cgtcgattcc aactcgcggg
     4081 gcgagcgaat cctgtacaga tccacgtggg caagcggcag acgcccttga tcatattcgc
     4141 gaatcaccac gaaggtcggg ctgcttacag cggtcggcga tgcaccgagg ccctgcgcga
     4201 ttccgcgaac gagtgccgtc ttgccggctc cgagcggtcc atagagcgcg atcgtttctc
     4261 ctccgcgcag cacgcggccg atcgtttcac ctaaccgagt cgtgtgcggg tgcgatgtgg
     4321 acaccagttt ccacggcagg tttgctgtga gtgcgcggcg agcggcaggc tgttgcgcct
     4381 tagctggtcg ggtcacgcta cgtcgttgtg gtccgctgga gtgcataagg aatctgagcg
     4441 atgaggtcac tggcgaccat cccgggttgc ccgaggtgtt gggcggccaa gtcgccggct
     4501 gagccatgaa aataggtcgc ggcgcaggcg gcttcccatg gggggacccg ttgtgccaat
     4561 aaccctacaa tcatgccggt taagacgtca ccggttccgg ctgtggccat gccgggatta
     4621 ccggtcggac aaatggcgac aaacccatcg ggtcgggcga caacggttcg tgcacctttt
     4681 aagacgacga acacgccgcg ttctcgggcg aaccgtctgg ccgtacctat tcggtcagcg
     4741 ttgacactct gcgtggtagc atcaatttcg agccgggcca tctcaccagg gtgtggggtg
     4801 agaataggag gagttttaca ttcggtcagc aacgaggccc tgccggtcaa ggcgttgagc
     4861 gcatcggcat cgaggacgga tggccggtcc agatgtttca tgagggactg tacgagttcg
     4921 acggtctcgg gatgcgttga aagcccaggt ccgatggcga tcgccgtgcg tgcctgaata
     4981 aaggctaaga cacgattgag tcccgagcga gccagtgtac gcgccttcgt ttccgggaga
     5041 ggcattgtca tggcttacag taactttgct tccagcacat cgttgacgct gctgggagtg
     5101 gccacggtga ccaggccggc tccaacacgg agtgccgctc gggccgccaa agccgccgcg
     5161 ccggtcttcc ccacggaccc
//
LOCUS       DATIPF010000127         7958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1528088, whole genome shotgun sequence.
ACCESSION   DATIPF010000127 DATIPF010000000
VERSION     DATIPF010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528088"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VLL06_03270"
     CDS             <1..255
                     /locus_tag="VLL06_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30016.1"
                     /translation="DAVIADEHMPGFTGSEFAKFCRMTWPNTPLILLIRDRNSLTDYA
                     EESGAATCICTPYEVAMLLSVLGRVTQPDSTEHASSQSLR"
     gene            328..741
                     /locus_tag="VLL06_03275"
     CDS             328..741
                     /locus_tag="VLL06_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS30017.1"
                     /translation="MDVHDTKILVAEDDESNRNQLADLLEQAQYEVHLAADGHEALEF
                     MFRGVVDVVVTDWHMPRLSGADFLSLSRILWPDIPVIIVSAYATPSLEGIPRGAFAWL
                     IKPYTREELLQVLRMAVQTTALRHRKQLVTTAVLP"
     gene            944..1567
                     /locus_tag="VLL06_03280"
     CDS             944..1567
                     /locus_tag="VLL06_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30018.1"
                     /translation="MITRTDSGGLVLGSLLMILGCSTAPTPATNPDAVRQHADKAFQQ
                     LGAQEHPQQQGDPQSTPQVSNSEGTPSIPAPSAQKGVHIPSAEIGPTDGEHIRATGYG
                     DLAKGLFLCQHSADLAARVELSKLVRVQVTEKSVDRIRERTGKDAEQDIEVVREGLVN
                     EVLTGVRIVNRNVDKEAGTCSSTAVIPKKNLSTTPVSSGDANLSAPH"
     gene            complement(1799..2086)
                     /locus_tag="VLL06_03285"
     CDS             complement(1799..2086)
                     /locus_tag="VLL06_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30019.1"
                     /translation="MKRDVLVTSLVKQKVVQKLAVDRQKDALGRLEHLESQMAKLSEL
                     VREHAEDVDRLVRIVAEDRDVIRRQLLRKHHEQVRVRKHLRDSNSKVGLDY"
     gene            2215..3453
                     /gene="glgC"
                     /locus_tag="VLL06_03290"
     CDS             2215..3453
                     /gene="glgC"
                     /locus_tag="VLL06_03290"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HSS30020.1"
                     /translation="MPNMFTMVLAGGKGERLYPLTEHRAKPAVPFGGKYRIIDFTLSN
                     CLNSGLRKVAVLIQYKSHSLDRHIRTGWSVFNTELGEFIASIPPQQRISEEWYQGTAD
                     AVYQNMFLLDTEQADYVLILAGDHIYKMNYMEMFYWLVAKSADAVVGAIDIPIEEANR
                     FGVIHVDQDYRITDFQEKPAHPPSIPNDPTHAFASMGIYLFRTSVLREHLTANAQEGG
                     SHDFGKDIIPRMIRDQRVYAYKFIDANNKDVKYWRDIGTLDAYWEANMDLVAVDPLFN
                     LYDQQWPIRTYQGQFPPAKFVFAQDYQGGRMGVSLDSIVCGGCIFSGGRVQNSVVSPN
                     VRVQDNADVRESIIMENVVIGARSRIRRAIIDKDVIIPPNSEIGYDREADAQRFTVTE
                     SGLVVIAKGMKLHASLDSSG"
     gene            3428..5287
                     /locus_tag="VLL06_03295"
     CDS             3428..5287
                     /locus_tag="VLL06_03295"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HSS30021.1"
                     /translation="MPPSIHPVDLILALRDKHLTPAIVFLTSRRACDEAMQAFDHSNI
                     LLPPPRQQAIATVLEKVIVQYPSIAEHPLIPTVQRIGVAAHHAGHLPSWKIAVEELMR
                     HGCLDAVFATTTLAAGVDFPARTVIITQSSIRKSRDFTDLTIGEVQQIAGRAGRRGKD
                     LVGFAIVTPSPYIDLHVLTKGFTGHPEAINSQFVISYPMVLNLLKAHPLDQIQPILAK
                     SFAQFQLNQRAEVLERKLDDLRDQMDPFGPRVCTDWITVWQTFDQVRRQKTHRAPVRR
                     HEPPEVAARFHFLTPGRVVGIGRSRGVVLRQYRSKGQKSPMITALRLGGGITESPAST
                     VTEVSDRVLDCVDAPVYPWCTPESVEELLRHLEELPGRLPELPILVPKDDEPIPDAIV
                     QTLGDFACPTCPSRAACQRDYPRASKLRQEQQRHLKSIQALRTSLWHRFQEKIDVLQQ
                     FGYLTPTAHLTDDGEWARLIRIDHSLLITELIRADAFSGADPSMLAAVMSSIAHDDDR
                     PGAFPRVSSGLGSLLGQVRKLAVSLSPHEDPPLLRADIAAVTERWIGDPTLTWIGLCK
                     STTMAEGDIYRLLARTLEFLSQLHTLKTTHPGLADTASRAIANLRRGVLEELP"
     gene            5298..6359
                     /locus_tag="VLL06_03300"
     CDS             5298..6359
                     /locus_tag="VLL06_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSS30022.1"
                     /translation="MTTQELEQLLAQQPIALLHRLARGRVQRHFRAGKRRLIEVLLRH
                     SAENREGFESDLMTLIGERQARPVPPAPQPPAGRKPKPSSPQHRTIHKPAEQDHEPAI
                     TLSSWLEGLGIPPPQTFIPDAWQEEAVAKLRENDVVVSVPTGSGKTYVAVEAARRAMA
                     ENRTVIYTSPLKALSNTKYTEFSKIFGPDQVGILTGDRRDNGQAPLLIMTTEILRNLL
                     YDAGGGEIDIRLDTLGLVILDESQYIADPERGVVWEETIIFCPSQARLLLLSASIGNP
                     HDIAEWLTSIRPTPCHLVRHSTRTVPLRAGYLHPNGKLTPLFRTVGIPHGHPSHLHPE
                     AKRLFAQYEDETLPTGRRR"
     gene            complement(6377..7372)
                     /locus_tag="VLL06_03305"
     CDS             complement(6377..7372)
                     /locus_tag="VLL06_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30023.1"
                     /translation="MIGWSRLIGLCVIGCSVATLSPFMTAAQEPAEERGPRVELSAGT
                     WFSVGDTRWAHNASSIPGLGNPTSKLTYKDVGTTVVDLTAKVWVNPRVFGRLNFGYAG
                     IGGGRLADDDFGSNGGQRLFSRTNSDLSGDNMWYLNADGGIRVAEYPNHRGWLDLFGG
                     YQFWHTKYEATGVTQVACDPSAIPGLSPCAPPGASGFSGQTVITNTTNWHSIRVGAST
                     EYRLTRRLSMLATLAFIPASLLDNKDVHHLRGDLSQDPSFSMVGYGVGADADIGARFM
                     ITTNLAANLGYRMYYNRVLSGHLTTHPAVGSTESFPLTQFESLRHGFTASLSLIF"
BASE COUNT         1798 a         2398 c         2106 g         1656 t
ORIGIN      
        1 gacgcagtca tcgcagatga gcacatgccc ggattcaccg gcagcgagtt cgccaagttc
       61 tgtagaatga cgtggccgaa cactcccctc attctcctaa taagggatcg caactccttg
      121 acggactatg cagaagaatc cggtgcggca acctgcattt gtacacccta tgaagtggcc
      181 atgctcctga gcgtgctggg cagagtgacg caaccggatt cgaccgagca cgcatcttcc
      241 caatcgctga gataacatac tgaaagtcgg gagcgggcat tacaatattt ccgcacatac
      301 atgcgatccg acaaggagga gcatacaatg gatgtccacg acacaaaaat tctggtcgca
      361 gaagatgacg aatccaatcg aaaccagctg gcagatctgc tcgaacaggc acagtacgag
      421 gtccatctcg ccgctgacgg gcatgaggca ttggagttca tgttcagagg agtggtcgat
      481 gtggtcgtga cggactggca tatgcccaga ctgagcgggg cagacttcct ttccctcagc
      541 cgaatcctct ggccggacat tcccgtcatc attgtttcgg cctatgctac cccttctctt
      601 gaaggaattc cacgaggggc cttcgcatgg ctcatcaaac catatacgcg ggaagaattg
      661 ttgcaggttc tccgaatggc tgtccaaacg accgcgcttc gccaccggaa gcaattggtc
      721 accacagcag tactgccatg accacgtcac gtctccttca tccatcattg ggtgcctggt
      781 gtggagccgc gttccctatc ccatcaatct gatgatcatt acatcgtggg ccgatcgtgc
      841 aattctgccc ttccatgagg gtgacctccg agcggtcagc cgatccatcg gattgacagg
      901 ctgccagaag agacgtaggc tgatctgacg tgaaggcgag ggtatgatca caaggactga
      961 cagcggggga ctggtcttgg gcagcctcct catgatactc ggctgctcga cggcaccaac
     1021 tcctgcaacg aaccccgatg ccgtgcggca acatgccgac aaagcctttc aacagctagg
     1081 agcacaagaa catccacagc aacaaggcga cccccaatcc acgcctcaag tttcgaattc
     1141 tgaaggcact ccctccatcc ccgcaccatc cgctcaaaag ggagtgcaca ttccgtccgc
     1201 cgagatcggg ccaaccgacg gcgagcacat ccgtgccacc gggtatggtg atctcgcgaa
     1261 ggggcttttt ctgtgccagc actcggctga tctggccgcg cgagtggaat tgtcgaagct
     1321 cgtccgggtc caggtcacgg aaaaatcagt ggatcggatc cgcgagcgga ccgggaagga
     1381 tgcagagcaa gatattgaag ttgtccgtga aggcctggtg aacgaagtcc tgaccggcgt
     1441 gcggatcgtc aaccgcaatg tggataagga ggcgggcacc tgttcgagca cggcagtgat
     1501 cccaaaaaag aacctcagca cgactccggt ttccagtggc gacgcaaacc tcagcgcgcc
     1561 gcattaacga ttccctcgca ttactccacc gcgccgggaa aagctaccac ctgtgtttca
     1621 tcgaatgatg tctttcagcg cctgtctgtg cgcgtgaggg catgggcgga catgtccagc
     1681 aagctgctag gccatggaat catgaagaac gttctgcagc atgaagcgcg taatcgtggc
     1741 gaccgcgttt gagatcctaa cgggagggat cgatgggggg aagccgtggg acacaggctc
     1801 agtagtcgag tcccacctta gaatttgaat cacgcagatg cttgcgaacc cggacttgtt
     1861 cgtggtgctt gcgtaacagc tgacgccgaa tgacgtcacg atcctcggcg acgatgcgca
     1921 ccaatcggtc gacatcttcc gcatgctcac gaaccaattc ggacaacttt gccatttggc
     1981 tctcaagatg ctccaaccgc ccgagggcat ccttctggcg gtccacggcc agcttctgaa
     2041 ccaccttctg ctttacgagc gacgtcacca gtacatcccg cttcatgctc actcctttgc
     2101 tcgaataccc acgatctttc tctactctcc agagtagtat cgtctcagat ccttgcgatg
     2161 tcaacgagtg tgctttcttt ctatttcctc accgtcctgt tacaattctc ggcaatgccg
     2221 aacatgttca ccatggtgct ggcgggtggg aaaggcgaac gtctgtaccc tcttactgaa
     2281 catcgggcca agccggcagt tccctttggc gggaaatatc ggattatcga ttttacgctc
     2341 agcaattgcc tcaactccgg gcttcgaaaa gtcgccgtcc tcatccagta caaatcccac
     2401 tcgctcgacc gtcatattcg cacgggctgg agtgtcttca acaccgaact cggcgaattt
     2461 atcgcatcaa ttccacccca acagcgaatc agtgaagaat ggtaccaagg caccgccgac
     2521 gcggtctatc aaaacatgtt tctcctcgac accgaacagg cggactatgt cctgattctt
     2581 gccggcgacc acatctacaa gatgaattac atggagatgt tctattggct ggtcgcgaaa
     2641 agcgccgatg ccgtggtcgg cgccatcgat attccaattg aagaggccaa tcgattcggt
     2701 gtgatccatg tggatcagga ttaccggatt acggattttc aggagaagcc ggctcacccg
     2761 ccatcgatcc ccaacgatcc aacccacgcc tttgcgtcaa tgggcatcta tttgtttcgc
     2821 accagtgtcc tccgcgaaca cttgactgcc aacgcccagg agggcgggag tcacgacttc
     2881 gggaaagaca tcattccacg aatgattcgc gaccaacgtg tctatgccta taaatttatc
     2941 gatgcgaaca ataaagacgt gaagtattgg cgggacatcg gaaccctgga cgcatactgg
     3001 gaagcgaaca tggatctcgt cgcggtggat cccctcttca atctctatga ccagcaatgg
     3061 ccgattcgga cctatcaagg gcagtttcca ccggctaaat tcgtcttcgc gcaagactat
     3121 caaggcggca ggatgggtgt gtctctggat tccatcgtct gcggcggctg catcttctcc
     3181 ggtggccggg tgcaaaactc cgtggtctca ccgaatgtcc gtgtgcagga caatgcagac
     3241 gtgcgcgaat cgatcatcat ggaaaatgtc gtcatcggcg cacggagccg gatcagacgg
     3301 gccatcatcg acaaggatgt catcattccg cccaactcgg aaatcgggta cgaccgagag
     3361 gctgatgccc agcgatttac tgtcaccgaa tccggattgg tcgtgattgc aaagggaatg
     3421 aaactgcatg cctccctcga ttcatccggt tgatctgatt ctcgctcttc gcgacaagca
     3481 cttaacccct gccattgtgt tcctgacgtc gcgtcgcgcc tgcgacgaag cgatgcaggc
     3541 cttcgaccac agcaacattc tccttccgcc tccccgccag caggcgatcg ccaccgtgct
     3601 ggagaaggtg atcgtccaat atcccagcat cgccgagcac ccgcttattc cgaccgtcca
     3661 gcgcatcggt gtggccgcac atcatgcagg ccatctcccc tcgtggaaga tcgcggtgga
     3721 agagctgatg cgtcacggct gcctcgatgc ggtcttcgca accaccacac tggccgccgg
     3781 agtcgatttc cctgcgcgga cggtcatcat cacgcagtcc agcattcgca aatcacgcga
     3841 cttcacggac ttgacgatcg gcgaggtgca gcagattgcc ggccgcgcag gccggcgcgg
     3901 gaaagatctc gtgggcttcg ccattgtcac cccttccccg tatatcgatt tgcacgtgct
     3961 gaccaaaggc tttaccggcc atccggaagc gatcaacagc caattcgtca tcagctatcc
     4021 gatggtcctg aacctcctga aagcgcatcc cctcgatcaa atccagccga ttctcgccaa
     4081 aagctttgcg caatttcagc tcaaccagcg cgccgaggtt ctggaacgca agttggacga
     4141 tctgcgcgat cagatggacc cattcgggcc gcgcgtctgc accgactgga tcacagtatg
     4201 gcagaccttc gatcaggtcc gccggcagaa aacgcaccga gcccccgtca ggaggcacga
     4261 acctccggaa gtcgccgcac gctttcactt tctcaccccc ggtcgcgtcg tgggtatcgg
     4321 ccgcagccgg ggcgtggtcc tccggcaata tcgcagcaag ggacagaaga gcccgatgat
     4381 tacggcattg cgtctaggtg gcggcattac cgagtctccc gcctccaccg tgactgaagt
     4441 atccgaccgc gtcctcgact gcgtggatgc gccggtctat ccctggtgca caccggagag
     4501 cgtggaagag ttactccgtc acctggagga attgccgggc aggctgccgg agcttcccat
     4561 cctggttcca aaggatgatg agccgattcc ggatgccatc gtgcaaacct tgggcgactt
     4621 tgcctgccca acctgtcctt cgcgggctgc ctgtcagaga gactatcctc gcgcgtctaa
     4681 acttcgccag gaacaacagc gccacctcaa atcgattcag gcactgcgta caagcctctg
     4741 gcaccgtttc caggagaaga tcgacgtcct gcaacagttc gggtacctca cgccgacggc
     4801 acatctgacc gatgacggcg aatgggccag gctcatccgc atcgaccatt cgttgctcat
     4861 cacggaactg atccgagccg atgcgttcag cggagcagac ccctcgatgc tggccgccgt
     4921 gatgtcgagc attgcgcatg acgacgatcg ccccggtgcc tttcctcgcg tcagttccgg
     4981 attgggttca ttgctcgggc aggtccgaaa actggcggtg tcgctttcgc cgcatgaaga
     5041 ccccccactc ttgcgagcgg acatcgctgc ggtcacggaa cgatggatcg gcgatcccac
     5101 gctgacttgg atcggtctct gtaagagcac cacgatggct gaaggggata tctatcgtct
     5161 cttggcacga acattggaat tcctgtcaca actgcacacg ctcaaaacca cgcacccggg
     5221 attggcagac accgcctccc gggccatcgc caacctacgc cggggcgtgc tggaggagtt
     5281 accgtaaaga gagggatatg accactcaag agctcgaaca actcctggct caacaaccga
     5341 ttgcgctgtt gcacagactg gcgcgcgggc gtgtgcagcg gcatttccgc gcaggcaagc
     5401 gtcgcttgat cgaggtgttg ctgcgccact ccgcagagaa ccgtgaaggg tttgagtccg
     5461 acctgatgac cttgatcggc gagcgacagg cccgccccgt tccacccgcg ccgcaacccc
     5521 ctgccggacg gaagccgaaa ccctcttcac cgcagcatcg cacgattcac aagccggccg
     5581 agcaggatca tgagccggcc attaccctct ccagctggtt agagggattg gggatacccc
     5641 ctccgcagac ctttatcccg gacgcctggc aagaagaggc cgtcgccaaa ctgagagaaa
     5701 atgatgtcgt cgtgagcgtc ccgacgggaa gcggaaagac ctatgtcgct gttgaagcgg
     5761 ctcgtcgagc catggcggaa aatcggaccg ttatctatac atcgccgctc aaagcccttt
     5821 ccaataccaa gtacacggag ttttcaaaaa tattcggtcc ggaccaggtc ggcattctga
     5881 ccggtgatcg gcgcgacaac ggacaagcgc ccctgctgat tatgacgacg gaaatcttgc
     5941 ggaatctgct ctacgatgcc ggaggcggtg agatcgacat acgattggat acgttaggtc
     6001 tggtcatttt agatgagtcg caatatatcg cagatccgga acggggcgtt gtctgggaag
     6061 aaaccatcat cttttgccca agccaggcac ggctgctctt actttccgcg tcaatcggca
     6121 acccccacga tattgccgag tggcttacgt cgatcagacc gactccctgc cacttggttc
     6181 gccacagtac gcgcaccgtc ccgcttcgcg ccggctactt acatccgaac ggcaaactca
     6241 caccgctctt ccgcaccgtc ggcattcccc acggccatcc aagccacctc catccggaag
     6301 ccaaacggct ctttgcccaa tacgaagacg aaacgctacc gacaggacgc cgccggtaac
     6361 cgtcgagaat agacgcttaa aatatcaggc tgaggctagc ggtaaagcca tgccgcaatg
     6421 attcgaactg agtgagggga aatgattctg tagatcctac agcaggatga gtggtgaggt
     6481 gaccgctcaa gacccgatta taatacatac ggtatcccag gtttgccgca aggtttgtcg
     6541 tgatcataaa acgtgcgccg atgtccgcat cggctcccac cccataaccc accattgaga
     6601 aactcgggtc ctgactgaga tctcctcgca ggtgatgcac atccttattg tcgaggaggc
     6661 tggctggaat gaatgcgagc gtggcgagca tactgaggcg tctggtcaac cgatattctg
     6721 ttgaagcacc aacccgaatc gagtgccaat tggttgtgtt ggtgatgacc gtctgtcctg
     6781 agaaaccact tgcgccaggt ggagcacagg gggacaagcc tgggatagca ctcggatcgc
     6841 aggccacctg cgtcactccc gtggcttcat atttcgtgtg ccaaaattga tatccgccga
     6901 acaggtccag ccaaccccga tggttcggat attcagcgac cctaatgccg ccgtccgcat
     6961 tcagatacca catgttgtca cctgataggt cgctattggt acgcgaaaac aacctttgac
     7021 ctccgtttga tccgaaatcg tcatccgcga gtctgccacc accgataccg gcgtacccaa
     7081 agttcaatcg tccaaacact cgtgggttaa cccatacttt ggccgtgaga tcaaccacgg
     7141 tcgtccccac atccttatag gtcagcttgg acgttggatt acctaatcct gggatcgatg
     7201 acgcattgtg tgcccatctg gtgtcgccga cactgaacca ggttcccgcg ctcagttcaa
     7261 ctctagggcc tcgttcctcg gccggctcct gagcagcggt catgaagggg gagagtgtcg
     7321 ccaccgaaca accgatgacg cacaggccga tcagtcgact ccacccaatc atcgtctcac
     7381 tcctccgcgt tgaataatca ctgtgtccac acagcgctct acggtttcag gcatcacgcg
     7441 ctgacaggcg gatcttatat catcttcgat gaatttccgc tggagatatg ccgagaggac
     7501 aacaagccat gacccgatca gcagcacaag tatggatccc aggacccaat accagacaaa
     7561 ggattgtggg ctcggcatgt gccccgccac ctcagcaggt gaactctcct cgggaactgc
     7621 ttgtaagtgt ggcatcgagt tcatacatcc ttctgcagat tgtcaagaag tctacacgag
     7681 atgcggagtt ctgaatactc ttttggaatt ctcatctaca tgtagttgac cggacgatgg
     7741 cacaagtcga acgactgtag acactgcatg ctcaagccta cgaccatcta gattgctcca
     7801 gctgtgtgtt tgcacctaca gcccatctct cgccctattc cagcaaggcg tttgcttgct
     7861 gagccaaaga aaatcgttcc ggcacaggca ttgctctatg tctgctcaac caacgccggt
     7921 gcacccctct ccggtgaaac agagtacagc gaaaggga
//
LOCUS       DATIPF010000128         4256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_290273, whole genome shotgun sequence.
ACCESSION   DATIPF010000128 DATIPF010000000
VERSION     DATIPF010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290273"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VLL06_03310"
     CDS             complement(<1..945)
                     /locus_tag="VLL06_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HSS30024.1"
                     /translation="MSTENPSSTRVPESSTDPVDQVIRYHVQTKHHFNRYARALGYLD
                     WANQPDPFRRYNGAQLIRLPLLRPEEEPVSPSYDSMYQPGLVQTQPVTVQTLSRFFEF
                     ALGLSAWKKAGESEWALRSNPSSGNLHPTEGYAVFPQIDGLDLKSGLYHYAPKEHGLE
                     CRANLPAEQVALLLAPFPPAAFVFGLTSVHWREAWKYGERAFRYCNHDVGHAIGSARI
                     AAATLGWNMALLEGVAHNTIAMLLGTDRTGDFVDVEPEHPDCLVVVWPIEDVKREASI
                     VNREELTEVPLFLDSAVVKELAGGKWNGKANRLSGEHGV"
     gene            1098..1520
                     /locus_tag="VLL06_03315"
     CDS             1098..1520
                     /locus_tag="VLL06_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30025.1"
                     /translation="MQFDAALAAQAALQESETDLGADWDTAVELESTFSSNAGSTARG
                     AYEGLLSLATRYPKAHSFQAFCIYITWQQVTEETLARHFQTGMRLSEAYLASREGKDQ
                     QHIDYVTELLESFRGGLGLDEEDEIQVEFRKDTPKGGD"
     gene            1748..2512
                     /locus_tag="VLL06_03320"
     CDS             1748..2512
                     /locus_tag="VLL06_03320"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS30026.1"
                     /translation="MADADKHRARIFLHRGQLVQAKVAYEQAVADDRLAEDQRALSDS
                     LGNLGNVCALSGNLDQAESCYREVLTIQRTERNQHAIAHTLVNLGNLHIGADHPEKAR
                     PYYLEALDLLRDLNDDRALGILYNNLALQEAREGRWEQAVTSFKQALDHHRIVGNEEG
                     LAVTYSQLGKCFLDQGDLTRAERCLNNASEHYIKLGNEPAEAAVLRILATLYDTRRDH
                     VSARRCLERVVALDQRYGLPELHTDLASLGTLKQSG"
     gene            2690..3292
                     /locus_tag="VLL06_03325"
     CDS             2690..3292
                     /locus_tag="VLL06_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HSS30027.1"
                     /translation="MPLLARVLATSALWALMTGTGVQAAEVSPNITVGTQEVGLTAGY
                     LLPHRLTTDHTTKQQGPAIMPSWMMTITDPVGDSWYRGQVSVGAEMVYIQFQEPFLTH
                     GVGFTPKIKYSFVALNRIRPYAEFAGGPFWTDLAGKIPEESSRFNFVLTAGFGVSYFL
                     TDQTAFNVGYRFHHISNAGTHYPNLGLNASLPFGGFSFFF"
     gene            3308..4057
                     /locus_tag="VLL06_03330"
     CDS             3308..4057
                     /locus_tag="VLL06_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30028.1"
                     /translation="MMGQTFSRPLIGGALIMALSLGTGCSSHWIDLAEQKGAPTPLQT
                     TVPSDLRVPLLIETIHTTQNGVALATPIAIEHQILGAFEATKLFSQHFQSGYAQPATN
                     QPHVTIRLTMNNSVEPHAGNAAWSGFIIGASMFILAPIVTLDYDYGTQMALEVERWDG
                     QTKQYQASSSGTAHYHLFAASQHLIDELKGQVTDTCLTQLLDQVIRDTPFYIAGEIAP
                     SETTIRSVSVGTKRSNTPVHPVSTSLEPATR"
BASE COUNT          982 a         1291 c         1062 g          921 t
ORIGIN      
        1 caccccgtgc tcgccgctca accggttggc ctttccgttc cactttcctc cagcaagttc
       61 cttcaccact gctgagtcaa gaaacagcgg aacctctgtt agctcctcac gattcacgat
      121 tgaggcttca cgtttcacat cctcaatggg ccagaccaca accagacagt caggatgttc
      181 cggctcaacg tcgacaaagt cgccggtgcg atcagtgccc aacaacatgg caatggtgtt
      241 gtgtgccacg ccttccaaca acgccatatt ccagccgagt gtcgccgccg cgatccgcgc
      301 tgatccaatc gcatgcccga catcgtggtt gcagtatcga aatgcccgct cgccatattt
      361 ccaggcttca cgccaatgca ccgaggtcag tccaaacaca aatgcagcag gaggaaacgg
      421 ggccagcaga agcgcgacct gttcggcggg aaggtttgcc cgacactcca agccatgttc
      481 cttcggcgcg taatgataga gccccgactt cagatcgagg ccatcaatct gcggaaatac
      541 cgcatagcct tccgtcggat gcaaattgcc ggacgacgga ttacttcgca acgcccactc
      601 cgactcccct gctttcttcc aggccgataa cccgagggcg aactcgaaga atcgggaaag
      661 agtctgcacg gtcacaggct gagtctgaac caatccaggc tgatacattg aatcatagga
      721 cggtgagacc ggctcttcct ccggcctgag caatgggagc cggatcaact gcgctccatt
      781 gtaacgccga aacggatcgg gctgattcgc ccaatccaaa tagccaagcg cacgagcata
      841 gcgattgaag tggtgtttcg tctgaacgtg atagcggata acctgatcga caggatccgt
      901 cgatgattca ggtactctcg tggaagatgg attttctgta ctcatagaaa ccgtatgggc
      961 agtctacaga gcggtatgaa ggaaagtaaa cgagaccgca acggttccct tgtgagaagt
     1021 ggtatctggt tttcttggca cattgacctt cctttcaccc tccgcgtatt ctatcccaac
     1081 acaattcgag gtcacccatg caattcgacg cagcccttgc cgcgcaagcg gcgcttcaag
     1141 aatcagagac tgatctcggt gccgactggg ataccgccgt tgaactggaa tccacctttt
     1201 cctccaatgc cggatcgacg gcccgggggg cctatgaggg gctgctctct cttgccactc
     1261 gttatccaaa agcccattcc tttcaagcct tctgcatcta cattacctgg cagcaggtga
     1321 cggaagaaac gctcgcccgg cattttcaga ccgggatgag gctctcggaa gcctatctgg
     1381 cttcacgtga aggaaaagac cagcaacaca tcgactatgt gaccgagctc ctggaatcgt
     1441 ttcgtggagg gcttggcttg gacgaggaag atgagattca ggtggaattc agaaaagaca
     1501 caccgaaggg tggcgactag tgaggagagt gccgagtcaa gaatgttccg aggctgtctg
     1561 ctgattgctg atagatgaca gcggttccgg gtctgattcg tcaaagccac agtgcgggca
     1621 gggcatacag accggtccat cacaacagtg atagatatat tccctcccac atttcttgca
     1681 gttcgtgtga gccatcgtga cgtgccgcac ggtagcaaac tttcagattt cgctcaagac
     1741 ctcctgtatg gccgacgccg ataaacaccg cgcgcgcatt tttctccacc gcgggcaact
     1801 ggtccaggcc aaggttgcgt atgaacaagc agtcgccgat gatcgcctgg ccgaagacca
     1861 gcgagccctc tccgactcgc tcggcaatct gggcaacgtc tgcgccctat ccggcaactt
     1921 ggatcaggca gaatcctgtt atcgggaagt cctcacgatc caacgtacgg aacggaatca
     1981 gcatgccatc gcccacacgc tggtcaatct tggcaatctc cacatcggcg ccgaccatcc
     2041 ggaaaaggcc cgcccctact acctcgaagc attggacctg ctgcgcgatc tcaacgacga
     2101 tcgggcactc ggcattctct ataacaatct ggcattgcaa gaagcccgtg agggacggtg
     2161 ggagcaggcg gttacctcgt tcaagcaggc cctcgaccat catcggattg tcggaaatga
     2221 agaaggattg gcggtgacct atagccaact tggcaaatgc tttctcgacc agggcgacct
     2281 gaccagagcc gagcgctgtc tcaacaatgc ctcagaacat tacatcaagc tcggcaatga
     2341 gccagctgaa gctgcggtgc tcagaatcct tgccaccctg tatgacactc gtcgagatca
     2401 cgtgtcagca cggcgatgtc ttgaacgcgt ggtcgctctc gaccagcgat atgggctacc
     2461 tgagcttcat accgacttgg cctccctcgg aacgctgaaa caatccggtt aacgtctcgc
     2521 gctctcttgc ctctccaaca gtttctgtcc agcattcttt cattttccgc cggatcgaat
     2581 tctacttatt cttggacaga actccaccct ctgctatctt tgagtggtca cacctgttgc
     2641 ttcaagcttt accatgaaat agcctacagg agattgacat ggagaccgca tgccacttct
     2701 tgctcgggtc cttgcaacat cggctctctg ggcgctcatg acggggaccg gcgtacaggc
     2761 cgcagaggtc tcgcccaaca ttaccgtcgg cacgcaagag gtcggactca ctgccggcta
     2821 cctgcttccg catcgactca cgacggacca cacaaccaaa cagcaaggcc cggccatcat
     2881 gccctcctgg atgatgacca tcactgatcc ggtcggcgat agttggtatc ggggtcaggt
     2941 ctcggtcggc gccgaaatgg tctatatcca gttccaggaa ccgttcctca cgcacggtgt
     3001 cggtttcacc ccgaaaatca aatacagctt cgtcgcactg aatcggatca ggccatacgc
     3061 cgaattcgcc ggaggtccct tctggaccga tctcgccggg aagattccgg aggaatccag
     3121 ccggttcaat ttcgtgttga cggccggatt tggggtctcc tacttcctga ccgaccaaac
     3181 cgcattcaac gtcggctatc gctttcatca tatttccaat gctggcacgc actacccaaa
     3241 cctcggactc aatgccagcc tgccctttgg cggtttctca ttctttttct gatcaggagg
     3301 acgcacgatg atgggccaaa ctttttcacg accattgatc ggtggagcgc tcattatggc
     3361 actgagcctt ggaaccggct gttcttccca ttggatcgac ctcgctgaac agaaaggcgc
     3421 gccgacacca ctacagacca ccgtcccgag tgatttacgc gtccccctgc tgatcgaaac
     3481 gattcatacg actcagaacg gcgtcgcgct ggccacgccg atcgccattg agcaccagat
     3541 tctcggcgcc ttcgaagcca caaaactctt ttcccagcac ttccagtccg gctatgcaca
     3601 gccggcaacc aaccaacccc atgtcacgat tcgactcacg atgaacaact ctgtggaacc
     3661 ccatgccggt aatgcggcct ggagtgggtt catcatcggc gcctccatgt tcattctggc
     3721 tccaattgtc acgcttgatt acgattatgg cacccagatg gcgcttgaag tcgaacgatg
     3781 ggacggccag accaagcagt accaggcttc ctcctccggc accgcacatt atcacctctt
     3841 cgcggcaagc cagcacctga tcgatgaact gaagggacaa gtgacagaca cctgcctgac
     3901 acagttactc gatcaagtta tccgcgatac tccattctat attgccggtg agatcgctcc
     3961 atctgaaaca actattcgta gcgtctccgt aggaacgaaa cgttcgaaca cccctgttca
     4021 tccggtatcg accagcctgg aaccagctac ccgataacag gctccagcaa tctgatcgaa
     4081 cacctcctag gccagacccc atgaggggtc tggcccccct cgttcacgca atgttaagat
     4141 acacatcatt tcttcagtga gaggcctatg gatcttgatg cgttcagaca gatggtcgcc
     4201 aaaaacccac gaggctttct cggccgtttc gggctcggga ataaactcat tcagga
//
LOCUS       DATIPF010000129         4487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1175257, whole genome shotgun sequence.
ACCESSION   DATIPF010000129 DATIPF010000000
VERSION     DATIPF010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175257"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VLL06_03335"
     CDS             <1..444
                     /locus_tag="VLL06_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30029.1"
                     /translation="GTDSAIPSGNAIVVLVLLRLFAFTREQRYHDRAEQLFRVFRSVM
                     EHNAYGSSAMLCALDWYLITPQEIVVVGTRGDTKTEALLSTIHRRYLPNRIVLVTEVF
                     PRAGEPGLPLAEGKMSVNGQPTAYICRRQTCSSPVTESQQLELLL"
     gene            455..706
                     /locus_tag="VLL06_03340"
     CDS             455..706
                     /locus_tag="VLL06_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30030.1"
                     /translation="MGIGDSGKGLYDHLYRRFFEGRASHQGYDFQHAPGGSAPAPVVT
                     FRDRLRWWTWGRWNRRKVLLAARDRFQQDILDIKKRPPT"
     gene            1075..1386
                     /locus_tag="VLL06_03345"
     CDS             1075..1386
                     /locus_tag="VLL06_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSS30031.1"
                     /translation="MGSKIYVGGLPYSTTEQQLSDLFAVHGAVTSSRIITDKFTGQSR
                     GFGFVEMSSDSEAQAAITALNGTELGGRTLTVNEARPQEPRTGGGGGGRGGRGGGGDR
                     Y"
     gene            1539..2876
                     /locus_tag="VLL06_03350"
     CDS             1539..2876
                     /locus_tag="VLL06_03350"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSS30032.1"
                     /translation="MSTFADMSLPQSLIQRLTQAGLTTPTPVQQAAIPLALKGRDIMA
                     QAKTGSGKTLAFLLPIIEQVMRREASAPAPGPYTKSGPGHASGPKFLVLAPTRELALQ
                     IEMELRKYAPASVTSLSVYGGTPIERHYRALQRPPLVVVGTPGRLLDLAGSGHLKLGG
                     ITFLVMDEADQMLDRGFLRDIQRIIRLLPAERQTLLFSATFSGDIQTLAQTIQRDPAR
                     ISVDPGISTPTTIVHAYYVVPGDQVRTQLVHTLLQTVTGGERSMVFCDQKYKVRRLAS
                     HLGGEPASVGALTGNHSQAQRERTLGAFRAGRLRSLVATDVAARGLDIQDVAQVIHYE
                     LPTNPNSYVHRTGRTGRAEKEGATFLILSQSEEREYLRMVRQLRIETKKLVLPVLVAL
                     PTPAPMSVNEQQAPRQRPRRSAEPSGRRFRDGENRGTTHRPGASVGGSRHSRY"
     gene            2994..>4487
                     /gene="uvrB"
                     /locus_tag="VLL06_03355"
     CDS             2994..>4487
                     /gene="uvrB"
                     /locus_tag="VLL06_03355"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HSS30033.1"
                     /translation="MPAFQLEAPFKPCGDQGQAIDKLVAGIAAGRKHQTLLGVTGSGK
                     TFTMANVVAQVQKPTLVLVHNKTLAGQLYQEFKQFFPHNAVEYFVSYYDYYQPEAYIP
                     QSNTYIAKDASVNDAIDQMRHAATAALLQQDDVLIVSSVSCIYGLGSPDVYHEMVVFL
                     EEGMEIRREKILAKLVEIQYARNDMDFHRGTFRARGDVIEIFPASNDAVSVRIELFGD
                     VVDAIHEIDPLTGKSLRKLPKVAIYPNTHYLIAPDRYERAITGIEEELDERVAYFKKQ
                     NQPVEAQRIAQRTKFDLEMIRAMGYCHGIENYSRHLSGRAAGEQPPTLLDYFPKDFLL
                     IVDESHATIPQVGGMYEGDYSRKRTLVEYGFRLPSAIDNRPLTFAEFERCLKQAVYVS
                     ATPGPYELGHAAGEVVEQILRPTGLMDPVIEVRPAKGQVDHLLGEVRTEVAQGGRVLI
                     TTLTKRMAEDLSEYYHDLGIKVRYLHSDIKTLERAEIIRDLRRGVFDV"
BASE COUNT          954 a         1211 c         1350 g          972 t
ORIGIN      
        1 ggtaccgata gtgcgattcc atccggcaat gccatagtgg tcttggtttt gttgagactc
       61 ttcgccttca cacgagagca gagatatcac gaccgggccg agcagctgtt ccgagtgttc
      121 cgaagcgtga tggaacacaa cgcctatggc tcatcggcca tgctctgcgc attggattgg
      181 tacctgatca cgccacaaga gatagttgtg gtcgggactc gcggcgatac gaagaccgag
      241 gcgttgcttt caactatcca tcggcgctat ctgccgaatc ggatagtgct ggtgaccgaa
      301 gtgtttcctc gagccgggga gcccggcttg ccgcttgccg aagggaaaat gagtgtgaac
      361 ggacagccaa cggcctatat ctgtcgacgt cagacctgtt cgtccccggt gacggaaagc
      421 cagcaattgg agctgttatt gtagggagga tgccgtgggc ataggcgatt caggcaaagg
      481 attgtacgat catctctacc gccgcttctt tgaggggcgt gcgtcgcacc agggctatga
      541 ctttcagcat gcgccaggtg gaagcgctcc agcaccggtc gtcacatttc gagatcgact
      601 tcgatggtgg acctggggtc ggtggaatcg gcggaaggtc ctcttggcag cgcgtgaccg
      661 gttccagcaa gatattctcg atatcaagaa gcggccaccg acataagaaa agaacgagaa
      721 gatacaggtg tgggaacgtc ccacgagtgt gaaggcctgg ccgaatgccc tgaagaagag
      781 cgaagctgcc acacgacttc ctagtcggat ggacgcacgg caccccacgt gattgtgtca
      841 tcgcttgcat ttgaatgggc taaggagtag agtgcggggc atgtatcatc gcgaaaggcc
      901 tggtggtgat accagaggga gtgacaaggc ctaagacaga tgttgttttg gttctgcccg
      961 ggaatttgat cggaagccag ccttgagccg ccatggtggt tgcatcttcg gccccttcct
     1021 tcctcacttt gttctcgagt ccaagacaac atatattctc aaaggaggag cccaatgggt
     1081 tcgaagatct atgtcggggg tttgccgtat tcaacaaccg agcaacagct gagcgatttg
     1141 tttgctgtgc atggagcggt gacatcatcc cggattatta ccgataagtt taccggccag
     1201 tcccgcgggt ttggattcgt ggagatgtcc tcggattcgg aagcgcaggc ggcaatcacc
     1261 gcgttgaacg gcactgagct gggtggccgc acgttgaccg tcaacgaagc gcgtccccag
     1321 gagccacgta ctggtggggg cggcggtggc cgtggtggcc gtggtggtgg tggcgaccgg
     1381 tactaagccg taacgagtgt tttttacacg tcggggctgc ctatcgggca gccccgctat
     1441 ttccttccta gaattgccca tcaactcgat gcgtttctcg gccctcgcct aggccagacc
     1501 gtgcagtccc aggaagtgcg tcccatacaa ggagttttat gtctacgttt gctgatatga
     1561 gtttgcccca gtcccttatc cagcgcctga cacaggcggg cctgacgaca cccacgccgg
     1621 tacaacaggc tgccattcca ctggcgctga aaggccggga cattatggca caagccaaga
     1681 ccgggagtgg aaagaccctg gcatttttgt tgccgattat cgagcaggtc atgcgacgtg
     1741 aggcctctgc ccccgcgcct ggaccctaca ccaagtccgg accaggccat gcgtccggac
     1801 cgaagttttt agtgttagca cccacgcggg aattggcgct tcagatcgaa atggagcttc
     1861 gcaagtatgc cccggcgtcc gtgacctcct tgtcggtgta cggcgggaca ccgatcgaac
     1921 gccattaccg tgcattgcag cgaccgccgc tggtggtggt cggtacgcca ggacggctgc
     1981 tcgatttggc cggttccggt cacttgaagt taggtggcat aacgtttctc gtgatggatg
     2041 aggccgatca gatgttggat cgtggattcc tgcgggacat tcaacggatc attcgtctcc
     2101 tcccggctga acgtcagacg ctcctattct ccgcgacatt ttcgggagac attcagacgc
     2161 tcgcacagac catacagcgc gatcccgccc gcatctctgt cgacccaggg atcagcacgc
     2221 cgaccacaat cgtgcatgcc tactatgtcg taccgggaga tcaggtgcgc acgcaacttg
     2281 tgcatacgtt gctccagact gtgacgggtg gtgaacggtc gatggtcttc tgtgatcaaa
     2341 agtataaggt gcgtcgcttg gcatctcatt tgggtggaga accggcttcg gtcggggcgt
     2401 taaccggcaa tcatagccaa gcgcagcggg aacggacgct cggtgccttt cgagccggcc
     2461 gactccggtc gctcgtagcg accgatgtgg cagccagagg cctcgatatc caggatgtgg
     2521 cccaggtgat tcactatgag ttgccgacta atccgaactc atacgtccat cggaccgggc
     2581 gaacgggtcg agccgaaaag gaaggagcga cgttcttgat tctgtctcag tccgaagaac
     2641 gggagtatct tcgaatggtg agacagttga gaatcgagac gaagaaactg gtgcttcccg
     2701 tacttgtcgc tctcccgact ccggccccta tgtcggtcaa cgaacagcag gctccgcgac
     2761 agcggccacg ccgaagcgcc gaaccatccg gcagacgatt ccgcgatggc gaaaaccggg
     2821 gaaccactca tagaccggga gcttcggtcg gtgggtccag gcattcacga tattaacaaa
     2881 ccgtggcgcc accggacccg gggaggggcc ggtggtgttt cgtgggttct cggtgtctgt
     2941 ctgggttctt tctgttatgc gcagcacggt ttccgatggt aagataactt cccatgccgg
     3001 cgtttcaact cgaagcccca tttaagccct gcggagatca aggccaggcc atcgataaat
     3061 tggtggccgg gattgcggct ggccggaagc atcaaacgct gctcggtgtc accggatccg
     3121 gcaagacctt taccatggcc aatgtggtcg cgcaggttca gaagcccacg ttggttctgg
     3181 tccataacaa gacgctggcc ggtcagctct atcaagaatt caaacagttt ttcccccaca
     3241 acgccgtgga gtatttcgtc agttactacg attactacca gccggaagcc tatattcctc
     3301 aaagcaacac gtatattgcc aaggacgcat ccgtgaatga tgcgatcgat cagatgcggc
     3361 atgcggcgac ggccgcactg ctacagcagg acgatgtcct gatcgtctca tcggtgtctt
     3421 gtatttatgg cctgggatca ccggacgtct accacgagat ggtggtcttc ctcgaagagg
     3481 ggatggagat tcggcgggag aagattctgg ctaagctggt agagattcaa tatgcccgca
     3541 atgatatgga tttccaccgc gggaccttcc gtgcccgcgg cgacgtcata gagatctttc
     3601 cggcttcgaa tgacgcggtg tccgtgcgaa ttgagttgtt cggtgacgtg gtcgacgcca
     3661 ttcacgaaat tgatccgctc actgggaagt cgctcagaaa gcttccaaaa gttgcgatct
     3721 atccgaacac gcactatctc attgcgccgg atcggtatga gcgggcgatt acaggcatcg
     3781 aagaagaact ggacgaacgc gtcgcctatt tcaagaaaca gaatcagcct gtggaggctc
     3841 aacggatcgc gcaacgcacc aagttcgatc ttgaaatgat tcgggccatg gggtattgcc
     3901 acggcattga gaactactcg cggcacctca gtggccgtgc agccggcgag cagccaccga
     3961 cgctcctcga ctattttccc aaagattttc tgctgatcgt cgacgaatcc cacgcgacga
     4021 ttccccaagt aggcgggatg tatgaaggcg actattcgcg aaagcgcaca ttggtcgagt
     4081 atggattccg gttgccgtcg gcaatcgata accggccgct cacgttcgcg gaatttgagc
     4141 gctgcctcaa gcaggcggtg tatgtctccg caacgccggg cccttatgag ctggggcatg
     4201 cggctggaga ggtggtcgaa cagatccttc gcccgaccgg tctgatggat ccagtcatcg
     4261 aagtgcgccc cgccaagggc caggtggatc atctgcttgg cgaagtgcgg actgaagtgg
     4321 cgcagggagg acgtgtgctg atcaccacct tgaccaagcg catggcggag gacttaagcg
     4381 agtactacca tgatcttggt atcaaggtgc ggtatctgca ctctgacatt aagacgttgg
     4441 agcgggccga aattatacgc gatttgcgcc gcggggtgtt cgacgtg
//
LOCUS       DATIPF010000130         9360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_730092, whole genome shotgun sequence.
ACCESSION   DATIPF010000130 DATIPF010000000
VERSION     DATIPF010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9360
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730092"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VLL06_03360"
     CDS             <1..191
                     /locus_tag="VLL06_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30034.1"
                     /translation="PFSPVTAAIAGYAARPSTATNINMLLMHRLHNNILLNEPSTLSL
                     QRPMGGNYNASHLKKTTR"
     gene            complement(355..1113)
                     /locus_tag="VLL06_03365"
     CDS             complement(355..1113)
                     /locus_tag="VLL06_03365"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HSS30035.1"
                     /translation="MSRMLVLVALFGLFTYCSTAWAGLPESSPMAPVPAGEFTMGSST
                     GDSDEQPERKVYVDAFMMDKYEVTVEQYAAFLQAKGIEPPADWKIMNQTTHQKRPAAN
                     MDSVDAALYCKWVGKRLPTEAEWEKAARGTDGRMYPWGNDAPTPLHANFGKTDWNNHG
                     ALAPVGSFEQGKSPYGLYDMAGNVWEWVSDWYDFRYYKNAPSKNPTGPSSGGTKVIRG
                     GAWNGNARALRSANRSVISPTDQGLTGFRCAKNP"
     gene            complement(1524..2186)
                     /locus_tag="VLL06_03370"
     CDS             complement(1524..2186)
                     /locus_tag="VLL06_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30036.1"
                     /translation="MSKYLTLLILSFGILLVSACGDSRIHSHGVYMLVDTSGTYDQEM
                     HKASKIINYLLATLNPGDSLAVAKVETRSFTEKDIIAKVTFDTRPSKATSQKRAFKTN
                     IAAFAKGVKGSAYTDITGGLIQGAEYLNETKAGIKTIVVFSDMQEELGKGTIRDFPIK
                     LNGIRIVALNVTKLGTDNADPRRYLDRLARWEASVRKAGATEWVVVNDLENNMESILT
                     TR"
     gene            complement(2183..3661)
                     /locus_tag="VLL06_03375"
     CDS             complement(2183..3661)
                     /locus_tag="VLL06_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30037.1"
                     /translation="MTTWNSWLFWPNNVAVSVLALAILAMLFLYAARKPMHGVIRSVC
                     ELLSQSTRFIARWLFLCADNLRVRNQSVLLAHGQESQAVVIDREFERVSNMIRKDMQE
                     FPALQRKMMEEATRIEDDYHKCGEVPPPPPEWVSALKSVAQIKSGGDIPRKLLEDINK
                     SIQKIHDQTVAEFRRSYEERHKILKAMQPSWRSVEKLTGEMEKKMLTLQTNAKQIDGH
                     MEKLEAIRAKDNKIEHALTVSGFVQLAISTLVLVIALGGAFINYKLIALPMSEMVGAS
                     DYISNNLKTSDVSALVIILMEASMGLFLLESLRITQLFPRIASMDDRMRHRLMLASLI
                     FLIILAAIESSLALMRDMLVVDKASLMHDLASTAPPVSNGLLTSIPMIGQMIMGFVLP
                     FALAFVAIPLENAMHSLRTVIGVFLVQAMRGLGFALRFAGVLFKRFPKVLEPAYDILI
                     VIPLMIERWVTGMRAGSTGTTGHTGSTGNTEEKELSKLGRAA"
     gene            complement(4133..4645)
                     /locus_tag="VLL06_03380"
     CDS             complement(4133..4645)
                     /locus_tag="VLL06_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HSS30038.1"
                     /translation="MADQAPHLFPVSPTPESQREGLLATAEFLAKILDTTVRIPGTRI
                     YLGLDPLIGLIPGLGDILVNLIGTVILILAARLQVPRIVIARMSLNLLINGTIGTIPI
                     LGDLFSVWFRSHARNAVLLREAATKPQRSSQGDWFYVAGIIGGTVVLLLLAITAVLWI
                     VVKLWAAIAL"
     gene            5045..7126
                     /locus_tag="VLL06_03385"
     CDS             5045..7126
                     /locus_tag="VLL06_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30039.1"
                     /translation="MRPLSIRSGHALVRRLIIILFLLSPWPVLAEEGATLPLSKISLY
                     SSGVGYFQHDGTVNSRAQLNLRLDTNQINDILKSLVVQDFGGGKVSTVTYGSRDPVTK
                     TLGSFGINLNGNPTLGQILTQVRGEPIEVTAPNPIIGTLLGVEKKTEFIGEGSQHRIV
                     EQEYVTLLTEDGFRSLPLANIQRIKLMNSALNAELHQALAVLTTNHDAQKKTVSITFD
                     GTGNRQARVAYLTETPVWKTTYRLVLDEDKAPYLQGWAIVENQTAQDWHHVKLSLVSG
                     RPISFAMDLYQPLYNPRPIVQPELYANLRPQTYGDAMDELKPMASAPAARSDMKKERL
                     LGKLAQGFAPSRSNAAAEAIASDMGIGSLEEGVASLAMAEDKGELFEYRIDQPVTLAK
                     HTSALLPIIGQTLQGHKVSLYNQRVNAKHPLNGYRLKNTSSLHLMQGPITLFDSGTYA
                     GDARIEDLSPGQDRLISYALDLKTEVEPTLEGGTQELATVSLKKGTMLISRRMIEDRT
                     YYVKNRDAKAKTVLIEQPYRADWKLVEPKEPTERTRDLYRFSVAVEAGKSTILRVKEM
                     LPVQESLLLMNSGIDQIVYYQQAKEVSPKVKEALQHVVQLRSKLDDVRAQRTRLDQRT
                     AEITAEHGRIRENMQRLQQNSELYNRYVKKLDQQETELEKLRKDIENLKNAEEEHRRE
                     LQNYVMNLDVA"
     gene            complement(7131..7706)
                     /locus_tag="VLL06_03390"
     CDS             complement(7131..7706)
                     /locus_tag="VLL06_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HSS30040.1"
                     /translation="MVESRTLLYSDFNCPFCYALHERLHEEHVLARCEWRGVQHASHL
                     PRPMKPWQGMLGAELRHEVTVVQRLAPGLPIALPPGKPNTKPAIEQAVALLQRDRSQG
                     MSFVRETYRAFWCDGKDISDPDVLRHLVEQAGGSPEGMDLTGAASRQVAQEWESAWHR
                     TGQAGVPLLVSPEGDLLVGCVPSDQIRQFFA"
     gene            complement(7872..8216)
                     /locus_tag="VLL06_03395"
     CDS             complement(7872..8216)
                     /locus_tag="VLL06_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS30041.1"
                     /translation="MACKFLQKLGCQVEVARNGREAVDSIAHTTYDAVLMDCEMPEMD
                     GYEATREIRRQEHTTSRHLPIIALTGHASPEDEQKCRRAGMDDVVTKPMTLPTLRTKL
                     EQLLARPDFRHR"
     gene            complement(8230..>9360)
                     /locus_tag="VLL06_03400"
     CDS             complement(8230..>9360)
                     /locus_tag="VLL06_03400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HSS30042.1"
                     /translation="LAEDAPNPIAEFDGDGSMLYANSAMVRLLNRGTSIQGRMEAVFP
                     PNLSDVLNDCRTSQQPTLLLEHHAEDCVLAWSFFPLGDSKQVRAYGSDISADVELRRA
                     KEAAEESARAKGIFLATMSHELRTPMNGVLGCTELLQDTSLSDQQRQLLEAMHRSAGS
                     LLVLVNDILDFSKIEAGKMSLEVADVELHSLIVDVVTLTSEAAKKKGLLVRVQLASDI
                     PVELRGDPVRLRQILFNLVGNAIKFTHQGEIHVSVKTVPRHQENSEAIVLQWTVQDSG
                     IGMTEDEQSRLFGVYAQANVSTARRYGGHRVGLDDLSPTGRAYGWSHHGGKYTGEGQC
                     LFVYHESVACDSAGHFRPCCKSAHCLAGGPHHTAPNSCRRRQ"
BASE COUNT         2165 a         2757 c         2463 g         1975 t
ORIGIN      
        1 gccccttctc accggtgacc gcagccatcg ccgggtacgc agcgaggcca agcaccgcaa
       61 ccaacatcaa catgcttctc atgcaccgcc tccacaataa tattctgctg aacgagcctt
      121 caaccctgtc cttacagcgc ccaatgggcg gaaactataa tgcctcgcac ctcaaaaaaa
      181 caaccaggta gggaagttga gcgcgacagg cacggcgagc ggaaaaggcg ggcctgaaaa
      241 gatggactag agctttcatg tcaggcatca accaagacac tcataacgga gtgggcgcac
      301 catgggaggg agcctaatcc ccctctcctt ggacgatatc aacagctgct cgctctatgg
      361 attttttgcg caccggaacc cggtgaggcc ctggtccgtc ggcgagatca cgctccgatt
      421 cgctgagcgc aaagcccgcg cattgccgtt ccaggccccg ccacggatca ctttggttcc
      481 gcccgatgat ggccctgtcg gattcttcga cggcgcgttc ttgtaataac ggaagtcgta
      541 ccagtcactg acccattccc acacattccc ggccatgtca tagagaccat agggactctt
      601 gccctgttcg aacgatccca ccggcgccaa tgccccatgg ttgttccaat ccgtcttccc
      661 aaagttcgca tgaagaggcg tcggcgcatc gttgccccac ggatacatgc gcccatccgt
      721 tccccgcgct gccttctccc actccgcctc tgtcggcagg cgcttgccga cccatttgca
      781 gtacagagcc gcatccaccg aatccatatt tgcagccggg cgcttctgat gcgtggtctg
      841 gttcatgatc ttccagtccg ctggcggctc aatccctttc gcctgcaaaa atgccgcata
      901 ctgttctaca gtcacttcgt acttgtccat cataaatgca tcgacataca ccttgcgctc
      961 aggttgttcg tctgaatcgc ccgtcgaact ccccatggtg aactcccctg ccggcaccgg
     1021 tgccatcggt gatgactccg ggaggcccgc ccaagccgtc gaacagtacg taaagagtcc
     1081 gaacaacgcg accaacacta gcatcctgct catgaacccc ctccgcatat tattgaaaga
     1141 cccacgactc ttgttctcat ttaacccaat gggccgaaac tataaagctt cgtacctcag
     1201 gaaggcaacc acattgatgc tgtgaggcgt gatctctcag cctcgagttc ctaagaggat
     1261 gagcccactt cgcggggatt ctacgaggcg cagacttcag agacgaggtt gcgaggggaa
     1321 aaacatgaaa ccgcatgttg cctgacggag tggagaacac gttcgcgtcc aggtgcgtcg
     1381 ccaccgcgct tgcgaggtcg agcgccattt gtatgatcct ggcgcgaaga aataataccg
     1441 caatgagttc tgaatttcat atcggcgggt gcagtgggag tcttcgcgag tcatgcaggc
     1501 tgactttgaa ataccacgca ggttcagcga gtggtcaaaa tggattccat attgttctcc
     1561 aggtcgttca ccacgaccca ttcggtggcg ccggccttcc gcacgctggc ctcccagcgg
     1621 gccaggcggt cgagatatcg acgggggtca gcattgtccg tgcccagctt ggtaacgttc
     1681 aaggcaacga tgcggatacc attcagctta atcgggaaat cgcgtatcgt tcctttgccc
     1741 agttcttcct gcatatccga gaacaccacg atggtcttga tgccggcctt ggtctcattc
     1801 aggtattctg ccccctggat caggccgccg gtgatatcag tataggcact gcccttgact
     1861 cccttggcaa acgccgcaat attggttttg aatgcgcgct tttgcgaggt agccttcgaa
     1921 ggtcgcgtgt caaacgtgac cttggcgatg atatcttttt cagtgaagct acgcgtctcc
     1981 accttggcca cagcaagcga atctcccggg ttcagcgtcg ccagcaggta attgataatt
     2041 ttggatgctt tatgcatctc ctgatcatag gtaccggaag tatccaccaa catatacacc
     2101 ccgtggctgt gtatgcggct gtcaccgcaa gcgctgacga gcaatatacc gaatgacagg
     2161 atcaagagag tcagatattt actcatgccg ctctcccgag tttggaaagt tccttttctt
     2221 ctgtattacc tgtactaccg gtatggcctg tagttcccgt cgaacctgca cgcatccctg
     2281 tcacccagcg ttctatcatc agtggaatga caattagtat gtcataggcc ggttcgagca
     2341 ctttggggaa ccgtttgaac agcaccccgg caaaccgcag cgcaaaacca aggccgcgca
     2401 tagcctgcac cagaaatacg ccgatgacgg tacgcagcga atgcattgca ttttccagcg
     2461 ggatcgccac gaacgccaat gcgaacggaa gcacgaatcc cataatcatc tgtccgatca
     2521 tcggtatgct cgttaataaa ccattcgaca cgggtggcgc ggtagaggca aggtcgtgca
     2581 tcagcgacgc cttgtcgacg accagcatat cgcgcatcag cgcgagggag gactcgatcg
     2641 cggccaggat gatcaaaaaa atcagagacg ctaacatcaa ccggtgacgc atacgatcat
     2701 ccatactggc aatcctcgga aacaattgcg tgatgcgcag cgattcgagc aggaacaggc
     2761 ccatcgacgc ttccatcaga atgatcacca acgccgacac atccgaagtc ttcaggttgt
     2821 tgctaatgta atcgctggcg ccgaccatct ccgacatggg cagcgcgatc agtttgtagt
     2881 tgataaacgc accgcccaag gcgatcacca aaaccaacgt agagatcgcc aactgaacga
     2941 aaccggacac agttaaagca tgttctatct tgttgtcctt ggccctgatg gcctctaatt
     3001 tttccatatg gccatcgatc tgcttcgcgt tggtctggag cgtgagcatc ttcttttcca
     3061 tttcacctgt cagtttttcc acagagcgcc aggatggctg catcgctttc aaaatcttgt
     3121 gccgctcttc ataggaacgt cggaattccg cgacagtctg atcatgtatt ttctggatgg
     3181 acttgttgat gtcctcgaga agcttgcgtg gaatatcgcc tcccgattta atctgcgcga
     3241 ctgatttgag tgcgcttacc cattctgggg gaggcggtgg aacctctcca catttgtggt
     3301 aatcatcttc gatgcgagtc gcttcctcca tcatttttcg ctgcaaggca gggaactcct
     3361 gcatatcctt gcggatcata ttgctcactc gctcgaattc ccggtcgatg acgaccgctt
     3421 ggctttcctg cccatgcgct aacagcaccg actgattacg cacccgcaag ttatccgcgc
     3481 acagaaacag ccagcgggcg atgaaccgtg tggactggga cagcagctca cagaccgagc
     3541 ggataacccc atgcattggc ttgcgcgctg catacagaaa cagcatcgcc agtatggcca
     3601 acgccaatac tgaaacggcc acgttattgg gccaaaacaa ccatgaattc caggtcgtca
     3661 ttaccggttc ctcccattac tcatacacga ttctcatacc gttacagaca gggcaccaag
     3721 atgaggtcgg ggcttgcaat cccacctggg ccgggttagg agccaatcgg atattgatta
     3781 ttcgctgcct cgcgagatct acagccaatt gcaccacgct cggtccaaaa agctcctcgc
     3841 tgtaggtcta caaatacacc tgggagtttt actgaactgc agcctcgcat tcagccgcat
     3901 ttccttcgcc tcctcacacg gacaatggga gaggggctcc tcgccgctct tcctgccata
     3961 cctgaacgtg tacgcgcaaa aggctgtgga gcacggcttt cccgatccta ctgagagcat
     4021 tattcatgcc gattggtcgc gaatcgctgg gaaatcgggc gtggggcatg ggacacaagg
     4081 cgagaagaaa gagatgcctc cgagtcagtc cactgatcac actatcccct gctcacaggg
     4141 caatcgcggc ccacagcttc acgacgatcc acaggactgc tgtaatggcg agcaacagaa
     4201 gcacgacagt cccgccgatg atgcctgcca catagaacca gtctccttgg ctgcttctct
     4261 gaggcttcgt cgcagcctct cgcaacaaca ccgcgtttct cgcgtgactt cgaaaccaaa
     4321 ccgaaaacag atcgccgagg ataggaatcg taccgatggt tccgttgatc aggaggttca
     4381 ggctcatccg tgcaatgacg attcgcggca cttgaagccg cgcggccagt atcaggatga
     4441 cggtcccgat gaggttgacc aggatatcgc cgagtccggg gatcaggcca atcaatggat
     4501 caagccccaa atagatccgt gtgccgggaa ttctgacggt cgtatccaga atcttggcca
     4561 ggaattcggc agtcgccagc agaccttctc gttgagactc gggggttgga gacacaggga
     4621 agagatgtgg ggcttggtca gccacgatgt gatagagcgg aaagttcgat gggccaacag
     4681 cggtccgttg aaccgtacca tgagtgatgc ggcttggcca actctctcta gcgggatttt
     4741 accggtctgc agccttgaac aacatgaaca cgccacgcag gaagctcaaa aaggctgtcc
     4801 agcaaggccg cagcgagcga agaggcgagg cgtacgtttc ggtacgttga gcctctgagc
     4861 gatgcgagaa cgccgcaggg gaagaggcgc gtctcggcgc gccaggggtt gggcgggtga
     4921 aaagagcgac tttttcagct tcctgcgaag gctgaaagga ttcgtccctt ggcgacatga
     4981 caagggtaag tcaaatcacc ttggccgccc cggcctggtg ccacacatta cgatggaggc
     5041 ctctatgcgt ccactgtcga ttcgatccgg tcacgccctg gtgagacgat tgatcatcat
     5101 attattcctg ctgtcccctt ggcctgttct agcggaagaa ggggccacac ttcccctctc
     5161 caaaatctcg ctctattcca gcggcgtggg atactttcag catgatggta cagtcaacag
     5221 tcgtgcacaa cttaacctgc gcctggatac gaatcagatc aatgacatcc tgaagagttt
     5281 ggttgtgcag gacttcggcg gcggcaaagt ctcgaccgtc acctatggtt cgcgcgatcc
     5341 cgtgaccaag acactcggca gtttcggtat taatctgaac ggcaacccga ccctgggaca
     5401 gattctgact caagtccgag gagaaccgat tgaagtcacc gcccccaatc ccattatcgg
     5461 cacattgttg ggcgtggaaa agaaaacgga gttcatcggt gagggttcgc aacaccgtat
     5521 tgtggaacag gagtacgtca cactcctgac agaagacggg ttccgctcgc tcccgctggc
     5581 gaacatccaa cgcatcaaac tgatgaattc cgccttgaat gctgaacttc atcaggcgct
     5641 ggctgtgctc accacgaatc acgatgccca gaagaagacg gtctcgatca ccttcgacgg
     5701 caccgggaac cgccaggccc gtgtggccta tttgacggaa acccccgtgt ggaaaaccac
     5761 ctatcgactc gtcttggacg aggacaaggc gccctatctg cagggttggg ccattgtgga
     5821 gaatcagacc gcacaggact ggcaccacgt gaagctctct cttgtctctg gacggcccat
     5881 ttccttcgcg atggatctgt atcaaccgct ctacaatcca cgtccgattg tccagcctga
     5941 gctctatgcc aatctgagac cccagaccta cggcgatgcc atggacgaac tcaagccgat
     6001 ggcttccgct cccgctgccc gcagcgacat gaagaaagag cgcttactcg gcaagttggc
     6061 acagggtttt gctccgagtc gatccaatgc cgcggcagaa gccatcgcct ccgacatggg
     6121 gatcggcagc ctggaagaag gcgtcgcctc cctggccatg gccgaagaca agggcgagct
     6181 gtttgaatat cgtatcgacc agcccgtcac cctcgcgaaa cacacgagcg cgctccttcc
     6241 catcatcggc caaacgctgc aggggcacaa agtgtcgctg tacaatcagc gcgtcaatgc
     6301 gaaacatcca ctcaacgggt atcggctcaa aaatacgtcg tccctgcacc tcatgcaagg
     6361 gcccatcaca ctgttcgaca gcggcaccta tgcgggcgac gcccgcatcg aagatctctc
     6421 cccaggccag gaccggctca tcagctatgc gctcgatctc aagaccgagg tggagcccac
     6481 actcgaaggc ggcacacaag agctggcgac ggtctcactc aagaagggca cgatgctcat
     6541 ctcacgccgg atgatcgaag accggaccta ctatgtgaag aaccgtgatg ccaaggccaa
     6601 gaccgtcctg atcgaacagc cctatcgtgc ggattggaag ttggtcgagc ccaaggagcc
     6661 gacagaacgc acacgggacc tgtatcgatt cagcgtcgcc gtcgaggcag ggaagagcac
     6721 gatcctccgc gtcaaagaaa tgctccccgt tcaggagtct ctcctattga tgaacagcgg
     6781 cattgaccaa atcgtctact accaacaagc gaaggaagtc agcccgaaag tgaaagaagc
     6841 actccaacac gtggtgcaat tgcgcagcaa gttggatgac gtgcgtgcac agcggacgcg
     6901 cctagaccaa cgtaccgccg agatcacggc ggagcatggc cgcatccgcg agaacatgca
     6961 gcggctccag cagaactccg aactctataa tcgctatgtc aaaaagttgg accaacagga
     7021 aaccgagctg gagaaactac gcaaggacat tgagaacctc aagaatgcgg aagaagaaca
     7081 tcggcgggag ttgcagaact acgtgatgaa tctggacgtg gcgtaatttt tcaggcgaag
     7141 aactgtcgaa tttgatctga cggtacgcag ccgacgagga ggtcaccttc cggcgaaacc
     7201 aagaggggaa ccccggcctg gcccgttctg tgccaggccg actcccactc ttgagcgacc
     7261 tggcggctcg cagcaccggt caaatccata ccctcaggcg agcctcctgc ctgctcaacc
     7321 agatgcctga gcacgtccgg atcggagata tccttaccat cacaccaaaa ggctcgatac
     7381 gtctcacgca caaacgacat tccctgtgat cgatcacgct gtagcaaggc caccgcctgt
     7441 tcgatcgccg gtttcgtatt gggcttaccg ggagggagcg cgataggcaa acccggcgcc
     7501 agccgctgga cgaccgtcac ttcgtggcgc agttcagccc ccaacattcc ctgccaaggt
     7561 ttcatggggc gaggcagatg cgacgcatgc tgcacaccgc gccattcaca gcgcgcaagc
     7621 acatgctctt cgtgcaaccg ttcgtgcaac gcataacaga acggacaatt aaaatcgctg
     7681 tagagaaggg ttcgtgactc gaccaatgtg tctactctcc taagaattcc gccagactgt
     7741 tgtaggtgta gtgcgatact gtactgacac gctcgaacac tatccattat catgaatgac
     7801 tgtcaagttt cttgctcgtg agttgcgcat cgagggagaa cggacgttcg ccaggcggct
     7861 tgggcattga ttcagcgatg acggaagtca ggccgtgcga ggagctgttc tagttttgta
     7921 cgaagggtcg gaagcgtcat gggcttcgtg acgacgtcat ccattccggc tcttcggcac
     7981 ttctgctcgt cctccggcga cgcatgcccg gtgagcgcaa tgatggggag atgcctggat
     8041 gtggtgtgtt cctgacgcct gatctcccgt gtggcttcat agccatccat ctccggcatc
     8101 tcgcaatcca tcagcacagc atcgtaggtg gtgtgagcaa tggagtccac tgcctcccgc
     8161 ccgttccgcg ccacctccac ctgacatcca agcttctgga gaaacttgca cgccacaacc
     8221 tgattgatct cattgtcgtc gacgacaaga attcggagcg gtgtggtgcg gtccgccagc
     8281 gaggcaatgg gcggatttac agcatgggcg gaagtggccc gctgaatcgc aggcaacaga
     8341 ctcgtggtat acgaaaaggc actgcccttc cccggtgtac tttccaccgt gatggctcca
     8401 cccataagct cgaccagttg gcgacagatc atcaagccca acccggtgcc ccccgtatcg
     8461 cctggctgtc gacacgtttg cctgagcata gactccgaat aatctcgatt gctcatcttc
     8521 ggtcatgccg atgcccgaat cctgtaccgt ccattggagg acgatggcct cggaattctc
     8581 ttgatggcgt ggcacggtct tcacggagac gtgaatctca ccctggtggg tgaactttat
     8641 ggcatttcct accagattaa acagaatttg ccgcaggcga accggatcgc cgcgcaattc
     8701 aacaggaata tccgacgcca attgaacccg aacgagcagc ccttttttct tcgccgcttc
     8761 cgacgtcagc gtgacgacgt ccacaatgag agaatggagt tcaacatcgg caacttccaa
     8821 tgacatcttt cctgcttcga tctttgagaa gtcgagaata tcgttgacga gcaccaagag
     8881 cgaccctgcc gaccgatgca tggcttccag taactgccgc tgctggtcgc tgagagaggt
     8941 atcctgaagc agttcggtgc atcccaatac gccgttcatc ggggtgcgca attcgtggct
     9001 cattgtggcc aggaagatgc cctttgcacg ggccgactcc tctgccgctt cttttgcacg
     9061 acgcaattcc acgtctgcgg aaatatcgct tccgtaagct ctgacctgct ttgaatcccc
     9121 taacggaaag aaggaccagg ccaacacaca gtcttcagcg tgatgttcca atagcagcgt
     9181 aggttgctgc gacgtgcgac agtcattcag cacatctgac aagttcggcg ggaatacggc
     9241 ttccattcgt ccctgaatgg aggtgccgcg attgaggagt ctcaccatag ccgaattggc
     9301 gtagagcatg ctcccgtctc cgtcaaactc tgcaatggga ttcggcgcat cctccgcaag
//
LOCUS       DATIPF010000131         4033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_291260, whole genome shotgun sequence.
ACCESSION   DATIPF010000131 DATIPF010000000
VERSION     DATIPF010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291260"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..699
                     /locus_tag="VLL06_03405"
     CDS             325..699
                     /locus_tag="VLL06_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HSS30043.1"
                     /translation="MWIMLWVVLMLVMTKAEVNGQVSISSVLSTAESSNLETVTLQGI
                     VQLTRPRRQELGGKCGAHSFMLSDDTGSVEVAIRRANRMVEPLREGDRVQITAQIKVF
                     RSQDNIPLRICVEAIKIQHLGS"
     gene            1329..1766
                     /locus_tag="VLL06_03410"
     CDS             1329..1766
                     /locus_tag="VLL06_03410"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS30044.1"
                     /translation="MYITQQEVGIHGVAFGQRLIDGKVEGQSVMDMKTEDRRATPRFR
                     VQFRTTVSGPTQPEGIGTMLDLSTGGCRLKIPFVIAPGLSLELRIYVPGLEWPLMIDG
                     AHVQWVSGQIVGLAFFRIRETEQQRLDEVITNLRAHRNEDDAR"
     gene            1859..>4033
                     /gene="glgB"
                     /locus_tag="VLL06_03415"
     CDS             1859..>4033
                     /gene="glgB"
                     /locus_tag="VLL06_03415"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HSS30045.1"
                     /translation="MSDLPLDQLEQLVQGHHWDPLAFLGAHPMAQNGSPAVAIRCFLP
                     EANRVSLLLNERNRPPIPMARLHEAGLFEAIVPGPLGAKPYRLQITNHAGQVSERYDP
                     YAFPPLLSDFELHLFAEGTFFKAYETMGAHLRTVQDIAGVHFVVWAPNAVRVSVVGDF
                     NQWDGRRHPMTNRGATGLWELFIPELTDGTLYKYEIRSRHHEAPLLKADPYAVASELR
                     PKTASIVRARTPYGWKDQSWMTARAQQDPLASPLSIYEVHLGSWMRIPEEGNRWLTYI
                     ELAAKLIPYVKNMGYTYIELLPITEHPFDGSWGYQTTGYFAVTSRHGSAEDFMFFVDA
                     AHQAGIGVLMDWTPAHFPDDPHGLSQFDGTHLYDHADPRLGYHPDWHSRIFNFDRVEV
                     RNFLLNSGLFWLDRYHIDGLRVDAVASMLYLDYGRREGEWIPNQFGGRENIGAVTLLK
                     DLNVLVHGHFPGAMTIAEESTSWPGVSRPTYAGGLGFTFKWNMGWMHDMLDYFAHEPV
                     HRPFHQNQITFGLLYAFNENFVLVLSHDEVVHGKRALLDKMPGDEWQRFANLRALYGF
                     MYGHPGKKMLFMGGEFGQWYEWNHDTSIQWHLCEFEPHAGLQRLTQDLNRLYRQEPAL
                     HELDHDWTGFEWIDFCDAAHSVILFLRKAKDQDNQILCVCNFTPVPRSGYRVGVPSSG
                     YYRELLNSDASVYGGSNMGNAGGLHTNAVPSHGMPHSLELTLQ"
BASE COUNT          862 a         1160 c         1112 g          899 t
ORIGIN      
        1 attgagtaaa ccgcagtcac tatatgtagg acatggcggc cattgcagta tggcccccaa
       61 tacattcccc ttccgaagta cttccttcag agcttttgtc ccaccctccg tgccgcgatt
      121 cttttcacag aacggacgtt ctacggtccg accataacca gcgaacgcga tgggctggtg
      181 cggaagacga tcattctgga agcgacctat gacaagtcat gaatgaggtc gtggcacaac
      241 tgaaatagcg gccaacagga attcgcgtga ttctggagtt cctcgtcctt gtcttctaga
      301 atgtgaacgt gggagggcgc cttcatgtgg atcatgctct gggtagtgct gatgctggtc
      361 atgaccaagg ctgaggtcaa tgggcaggta tcaattagca gcgtcctctc gacggcggag
      421 tcgtcgaacc tcgagaccgt cacgcttcaa ggcatcgtcc aactcacacg gccacgtcgg
      481 caagaattgg gcggaaagtg tggagcccac agctttatgt tgtctgatga cacaggttca
      541 gttgaggtgg caatccgtcg ggcgaatcgc atggtcgagc cgctccgaga aggcgatcgc
      601 gtgcagatca ccgcgcagat caaagtattc cgcagccaag acaacattcc gctccgcatc
      661 tgtgtcgaag cgatcaagat tcagcatcta ggctcgtaag cgcggtggga caaccgtacg
      721 atgtgccgca tggccggagt ctatttgcag tcatggtgag cagtatgagc tgcatccttt
      781 catgtgtgtg ttgggcggtg aggagcggcc gtcagcgtgg aagcgagtgg agatttacct
      841 cgatcgtgcc gtgaaattcc ccacgccgtt tccctgagaa agtcccacca gtgccacggt
      901 acaattcata accaagcggg atcatgaacg catcattcaa cagtttgatg gagtattgac
      961 gtttgacgat gacaagatct aggtcgaacc ggttcggctg gtcccgtctg tatgaaagat
     1021 gcccctcttc tcgccttcct cccacatgtt cttctacggc cctgatactc tgtgggcaaa
     1081 gagctgccac atctgaaaca ccgtggtact tggttcaaaa cggtgctgtg gtatccaact
     1141 atacattccg tggcatacaa ggtgagaacc gtcagacgtt gacgcgcatg caacgaagcg
     1201 acccatctgg atgaacctac acccggtgcc acgtggatct ggcacaaaca cgcgcggtag
     1261 ggtactcttg gacttggaga agacggggct gctgtggggt caacaatcgt gagttctcag
     1321 ctgatgtcat gtatataacg cagcaggaag tcggtattca tggagtcgcg tttggccaac
     1381 gtctcattga tggaaaggtt gagggtcaat cggttatgga tatgaagacc gaagatcggc
     1441 gggctacgcc ccgcttccgc gtgcaatttc gcaccacggt ttcaggcccc acacagcccg
     1501 aagggatagg caccatgctc gatttatcca cgggtgggtg tcggctgaaa atcccgttcg
     1561 tcatcgcacc gggtctctca ttggagttgc ggatctatgt gcccggcttg gagtggccgc
     1621 tgatgattga cggggctcat gtgcagtggg tgagcgggca gatcgtcggc ctggcatttt
     1681 tccgaatcag ggagactgag caacagcgac tcgacgaggt gatcacgaat ctcagagcgc
     1741 acaggaatga ggacgatgcc cgctgacctg cgggcattag ccgtgtgtgt acgttttcct
     1801 cttccattcc ttgctcaccc cccttacgat acgtctacgc actgaggact cgcttcgaat
     1861 gtcagacttg ccgctcgatc aactcgaaca actcgtccag ggacatcatt gggatcccct
     1921 cgcatttctt ggagctcatc cgatggccca gaacggttcc cctgctgttg cgattcgatg
     1981 tttcctaccg gaggccaaca gagtatcctt actcctcaat gagcgaaatc gaccgcccat
     2041 tcccatggcg cgtcttcacg aggctggact gttcgaggcg atcgttcctg ggccgctcgg
     2101 agccaagccc tatcgtctcc agatcaccaa ccacgcggga caggtctcgg aacggtatga
     2161 cccctatgca ttcccgccac ttctctccga ctttgagtta catctcttcg ccgagggaac
     2221 cttcttcaaa gcctatgaaa ccatgggcgc ccacctcagg acggttcagg atatcgctgg
     2281 agttcatttt gttgtctggg cgccgaacgc cgtgcgagtc agtgtcgtgg gtgacttcaa
     2341 ccaatgggat ggccgccgcc atcccatgac aaatcgcgga gcaaccggcc tctgggaact
     2401 cttcattccc gagctcacag acggcacgct gtacaagtat gaaatccggt cccgccatca
     2461 tgaggcaccc cttctgaaag ctgatcctta tgcggtcgca tcggagctgc gccccaaaac
     2521 cgcctcgatc gttcgtgcac gcacacccta cggctggaag gaccagtcct ggatgacggc
     2581 acgggcacag caggacccgc tcgcctcgcc cctctcaatt tatgaagtcc atctcggctc
     2641 ctggatgcgt atccctgaag aaggaaatcg ctggttgacc tatatcgagc ttgcggcaaa
     2701 gctcatccct tacgtgaaga atatgggcta cacctatatc gagctgttgc ctattacgga
     2761 gcacccgttc gacggttctt ggggctatca gaccaccggc tatttcgccg tgaccagccg
     2821 gcacggatct gccgaagatt tcatgttctt cgtcgatgcc gcacatcagg cgggaatcgg
     2881 tgtgctgatg gactggaccc ccgcacactt tccggacgac ccacatgggc tttcccagtt
     2941 tgacgggacg catctctacg atcatgccga cccgcggctc ggctaccatc cggattggca
     3001 tagccgcatc ttcaacttcg atcgcgtcga ggttcggaac ttcctgctga acagcgggct
     3061 gttttggttg gatcggtacc acattgacgg cttgcgcgta gatgccgtgg cctcgatgct
     3121 ctatctcgat tatggacgga gggaagggga atggattccc aatcagttcg gcgggcgcga
     3181 aaacatcggc gccgtcacat tgctgaagga cctaaatgtc ctggtccatg gtcacttccc
     3241 tggcgccatg accatcgccg aagaatccac gtcctggccg ggagtctcac gccctaccta
     3301 tgccggaggg ctgggcttca ccttcaagtg gaatatgggc tggatgcacg acatgctgga
     3361 ttacttcgcc cacgagcccg tccaccgtcc ctttcatcag aatcaaatta cgtttgggct
     3421 cctctatgcc ttcaatgaaa acttcgtgct ggtgctttcg cacgatgaag tggtgcacgg
     3481 gaaacgggcc ctgctcgaca aaatgcccgg ggacgagtgg caacgctttg ccaatctccg
     3541 ggccctctat ggcttcatgt acgggcatcc cggcaagaaa atgctgttca tgggtgggga
     3601 gttcggacag tggtatgagt ggaaccatga caccagcatc caatggcatc tctgcgagtt
     3661 cgagccacac gcggggctcc agcgcctcac gcaagacctg aaccggctct accgccagga
     3721 accggcgctc cacgagctcg atcacgactg gaccggtttc gagtggatcg acttttgcga
     3781 tgcggcccat tccgtgatcc tcttcctcag aaaggccaag gaccaagaca accagatatt
     3841 gtgtgtgtgt aacttcaccc cggttccccg ctccggctat cgagtcggcg tacccagctc
     3901 aggctactac cgggaactct tgaacagtga tgcctcggtc tacgggggaa gcaacatggg
     3961 aaatgcagga ggtctccaca cgaacgcagt cccctcccac ggcatgcccc attcgctgga
     4021 attgacactc cag
//
LOCUS       DATIPF010000132         4989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_822148, whole genome shotgun sequence.
ACCESSION   DATIPF010000132 DATIPF010000000
VERSION     DATIPF010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822148"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VLL06_03420"
     CDS             <1..564
                     /locus_tag="VLL06_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30046.1"
                     /translation="DVVDQALSMQSGARMSYMAIAVPPNTDQLVRETVTVPMSAGDTV
                     TQLLWSRLKTRQGVTVLAPSEAAKVLASQSTTQSPAGQLPAVAVAKQLKVDASLIGQV
                     LMYQERVGGRFGASPPATVGFEAKVIAADGQVLWVGNYYERQRPMIEDVMGFIQRRGM
                     FVTAEELATYGVEHLLLEFPFGTGEER"
     gene            570..1295
                     /gene="hisA"
                     /locus_tag="VLL06_03425"
     CDS             570..1295
                     /gene="hisA"
                     /locus_tag="VLL06_03425"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HSS30047.1"
                     /translation="MLVIPAIDLKDGRCVRLRQGDMAAETIYSDDIPAIARQWQQGGA
                     TLIHVVDLNGAVDGEPRNLSQIEAIIRTVSLNIQVGGGIRTIEMVRRYLGAGVTRVVL
                     GTAALTDRSFLEKACLEFPRRILLGLDARDGKVAVKGWTAVSETRATDLLKELAGYAL
                     GAVIYTDIARDGMLGGPNLEALREVVELSSFPVIASGGISRIEDLRAVQELGPRVEGA
                     IVGKALYDGKLDYRTAVTALRKA"
     gene            1325..2086
                     /gene="hisF"
                     /locus_tag="VLL06_03430"
     CDS             1325..2086
                     /gene="hisF"
                     /locus_tag="VLL06_03430"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HSS30048.1"
                     /translation="MLTKRIIPCLDVKDGRVVKGVSFVNLRDAGDPVEVATVYDREGA
                     DEICFLDITASHENRQTIIGVVEQTAAQVFMPLTVGGGIRTLEDIRALLKAGADKVSI
                     NTAAVQRPEFVREAAERFGTQCIVVAIDAKRRAAEAAGWEVFTHGGRRSTGLDVVEWA
                     KTMAQYGAGEILLTSMDQDGQQQGYDLALTAAVSEAVPIPVIASGGVGNLDHLYDGFV
                     RGKADAVLAASIFHFRTHTIPEAKAYLRQRGVAVR"
     gene            2083..2739
                     /gene="hisIE"
                     /locus_tag="VLL06_03435"
     CDS             2083..2739
                     /gene="hisIE"
                     /locus_tag="VLL06_03435"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HSS30049.1"
                     /translation="MMDVGQLKFDAQGLIPAVVQDWRDGTVLMVAFMNLEALQKTVET
                     KSVHFWSRSRQTLWEKGETSGHRLQIKDLFVDCDGDALLVKVEPVGPTCHTGERACFF
                     SRLDSPETKTSESWGGILERLSQMIQERKCQPTSESYTSKLLEGGLDRILKKVVEEAG
                     EVAIAGKGGKKDEIIYETADLLFHTLVALGYHSITLQDVYQELATRFGKSGLRKGTTS
                     "
     gene            2736..3080
                     /locus_tag="VLL06_03440"
     CDS             2736..3080
                     /locus_tag="VLL06_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HSS30050.1"
                     /translation="MSECLFCKIIARTIPAVLVYEDDLVVAFDDINPQAPTHTLVIPR
                     KHVASMAELQDSDVGLLGRLMLAGNTVAKQKGIAAAGYRFVVNTGAHGGQTVFHLHLH
                     ILGGRHLAWPPG"
     gene            3202..>4989
                     /gene="dnaG"
                     /locus_tag="VLL06_03445"
     CDS             3202..>4989
                     /gene="dnaG"
                     /locus_tag="VLL06_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HSS30051.1"
                     /translation="MGRGLISEDVINQVRDRVDIAEVVGHHVSLTRAGQNLKGLCPFH
                     QEKSPSFTVSPSRQIFHCFGCGAGGNVFTFLMRITGANFPETVRELGQKFGIDVPDSG
                     PNAGPQAAQASRLEQLNRAVTAWFQQNLRDGVVGATARDYLTGRGIQAETIDRFEVGC
                     APSEWDGLVKALSKQGFAQSDLAAAGLTVAREHASGAYDRFRARVMFPISDLRKRVVG
                     FGGRTLGEGTPKYLNSPDTLLFKKGQTLYALDLAREAVARLKTVIVVEGYFDAVALHQ
                     AGLTHTVATLGTALTAEHIQVLRRFASKVVLLFDPDQAGVRAALRGLDLFVNSGIGVK
                     VVTLPAGEDPDTYVRKEGSEAFARLEEQSPSLLDFSLEHCLSTAESGTIEGRIRSVDD
                     VLRILQKSEHPIEREERIRVVAERLGISQQRLIERYPALVQSEGSRPATVQSAGVLPA
                     MFKGASEERDLMYLLLHGHLTPADVQRLRPEAFSVPACRSLVEAALNHLDRDGRVGLR
                     LLLDAVIDHPDCGSLATELSMREDHFDDVKAHVAGCLETLDRKRAEAVFRNLIGQLKA
                     AEREGRAEDAQRLNTQVNELRMQKSGRLSI"
BASE COUNT         1050 a         1198 c         1646 g         1095 t
ORIGIN      
        1 gatgttgtcg atcaagccct ctccatgcag tccggggccc gcatgtctta tatggccatc
       61 gcggttccgc cgaataccga tcagcttgtt cgggaaacgg tcactgtacc gatgagtgca
      121 ggagatacgg tcacacagtt actgtggagc cgattgaaga ctaggcaggg tgtgacggtg
      181 ttggcaccaa gtgaggcagc caaagtactg gcgtcccagt cgacgacaca atctccagcc
      241 ggtcagttac cggcagtggc tgtggcgaaa cagctcaagg tggatgcctc gttaattggg
      301 caagtcctga tgtaccagga gcgagttggc ggtcgttttg gtgccagccc cccggcaacg
      361 gtgggttttg aagccaaggt cattgcggct gatgggcaag tactctgggt cggcaactac
      421 tatgagcgtc agcggccgat gatagaagac gtcatgggat tcattcagcg gcgtggaatg
      481 tttgtaacgg cggaagaatt ggcgacatat ggggtggagc atctgctcct cgaatttccc
      541 tttggaacgg gagaggaacg ctagcaacga tgcttgttat tcccgcgatt gatctcaaag
      601 atggccgctg tgtgcggttg cgtcagggcg acatggcggc cgagacgatt tactcagacg
      661 acataccagc tattgcgagg caatggcaac aaggtggtgc gacgctgatc catgtggtgg
      721 acctgaatgg agccgtggat ggggaaccgc gcaatctttc ccagatcgaa gcgatcattc
      781 gcaccgtgag cctgaatata caggtcggag gcggcatccg gacaatcgaa atggtgcgtc
      841 gctatttggg tgcaggcgtg accagggtgg tgctcggtac tgcagctttg accgatcggt
      901 cgttcctgga aaaggcctgc ctggagtttc ctcgccggat tctgttggga ctggatgcgc
      961 gtgatgggaa ggtggcggta aaaggttgga ctgcggtatc ggagacgagg gcgacggatc
     1021 tactcaagga gctggcaggg tatgccttag gcgccgtgat ttatacggac attgcccgcg
     1081 atggtatgtt gggcgggccg aatctggagg cgttgcggga ggtggtggag ctatcgtcct
     1141 tccctgtgat cgcttccgga gggatttcgc gcatcgaaga cttgcgagcc gtgcaggagc
     1201 tcggtccgcg ggttgaaggg gcgattgtgg ggaaagcgtt gtacgacggg aagctcgatt
     1261 atcggaccgc agtcacggct ctccgtaagg cctaagtggt gaagggtgag gtgaagacta
     1321 ggacatgctg actaaacgca ttatcccctg cctcgatgtc aaagacggtc gcgtcgtcaa
     1381 aggcgtgagc tttgtgaatt tgcgcgatgc gggagatccg gtcgaagtgg caaccgtcta
     1441 cgatcgcgaa ggagccgatg aaatctgttt cctggatatc accgcctctc atgagaatcg
     1501 tcagaccatc attggcgtgg tcgaacagac ggcggcgcag gtgtttatgc cgctaacggt
     1561 cggcggagga ataagaacgc ttgaggacat ccgggcattg ttgaaggcgg gggcggacaa
     1621 ggtgagcatc aatacggcgg cagtccaacg gcctgaattt gtgagggaag cagcggagcg
     1681 attcgggacg caatgtatcg tcgtggcgat cgatgccaag cgtcgcgctg cggaggcggc
     1741 agggtgggaa gtgttcaccc acggtggccg cagatcgacc ggtctcgacg tcgtcgaatg
     1801 ggcaaagacg atggcgcagt atggggcagg tgagatattg ctcacaagta tggatcagga
     1861 tgggcaacaa cagggatatg accttgcgtt gacggccgcc gtgtcagagg ctgtgccgat
     1921 tccagtaata gcgtcgggcg gtgtgggaaa tctggaccac ctctatgacg gattcgtcag
     1981 gggcaaggcc gatgcggtac tggccgcgtc catcttccac tttcggacgc acacgattcc
     2041 ggaagcgaaa gcctatcttc gtcagagggg agtggcggtt cgatgatgga tgtcgggcaa
     2101 ttgaaattcg atgcgcaggg actaattccg gcagtcgtgc aggattggcg ggatggcacg
     2161 gtcttgatgg tggcttttat gaatctcgaa gcgctgcaga agaccgtcga aaccaagtct
     2221 gtgcacttct ggagccggtc gcgccagaca ttgtgggaaa agggcgagac ctccggtcat
     2281 aggctgcaga tcaaggacct atttgtcgac tgtgatggtg atgcccttct tgtgaaggtt
     2341 gagccggttg gcccaacgtg ccacacagga gagcgggcct gtttcttttc gcgtctggac
     2401 tcaccggaga caaagacatc agagtcttgg gggggaattc tggagcgtct ctcccagatg
     2461 atccaagagc ggaagtgtca gcccacgagt gaatcctaca cctcaaaatt gctggaaggc
     2521 ggtctcgatc gtatcctgaa gaaagtcgtt gaagaagccg gcgaggtggc gattgcgggg
     2581 aagggcggga agaaagacga aattatctac gaaacggctg acctgctttt ccatacattg
     2641 gtggcgctgg ggtaccatag cattacgctt caagacgtct atcaggagtt ggcgactcgg
     2701 ttcgggaagt cgggattgag aaaaggaacc acctcgtgag tgagtgcctc ttttgcaaaa
     2761 ttatcgcaag gaccattcct gcggtgcttg tctatgagga cgatctggtg gtcgcgttcg
     2821 acgatatcaa tccccaggca ccgactcata cgctcgtcat tcctcggaaa catgtggcgt
     2881 cgatggctga gcttcaagat tcggatgtcg ggctgctcgg gcgtctgatg ctggccggta
     2941 atacggttgc caaacagaag gggatagccg ctgccgggta tcgcttcgtt gtcaacaccg
     3001 gtgcacatgg aggccagacc gtatttcatc ttcatctcca tattctgggc gggcgccatc
     3061 ttgcgtggcc ccctggttag tgccttttca cgacgttgac agatttctcc tccgtctgtt
     3121 agcctaaccg gtcaatttta ctaccactta cgtttacctt tcccacccgt gtcaggtggg
     3181 gaaataggag ccgcgactcc cgtgggccga ggcctgattt ctgaagacgt gatcaatcaa
     3241 gtcagagatc gggtcgatat cgccgaagtc gtgggacatc atgtgagcct gactcgggca
     3301 ggccaaaact tgaagggtct ctgtcccttt catcaggaaa aatcgccctc cttcacggtc
     3361 agtccttccc gccagatctt tcattgtttc ggatgcggcg cgggcggcaa cgtgtttacg
     3421 tttttgatgc ggatcaccgg cgcgaacttt cccgagacgg tccgtgaact tggacaaaag
     3481 tttggaatcg acgtgccgga cagcgggccg aacgcagggc ctcaagcggc acaagcgagt
     3541 cgccttgagc agctcaatcg cgcggtaacg gcgtggttcc aacagaattt gcgggatgga
     3601 gtggttggtg cgacggcaag ggactacttg accggccggg gcattcaggc tgagacgatc
     3661 gatcgatttg aagtcgggtg tgccccatct gagtgggatg ggcttgtcaa ggcgctgagt
     3721 aaacagggtt ttgctcagag tgatttggcc gctgcgggat tgacggtggc gcgtgaacat
     3781 gcgtctgggg cctacgatcg ttttcgcgcc cgtgtgatgt ttcctatttc ggatctgcgt
     3841 aagcgggttg tcggttttgg agggcgtacc ctcggtgagg ggactccgaa gtacctgaat
     3901 tcaccggata cgctcttgtt caagaaaggg caaacgctct acgcgttgga tcttgcgcgg
     3961 gaagcggtcg cacggctgaa gaccgtgatc gtggtggaag ggtattttga cgcagtcgca
     4021 cttcaccaag ccggactgac ccatacggtc gcgaccctcg ggactgcact gacggcggaa
     4081 catattcagg tgctgagacg gtttgcctcg aaggttgtgc tgttgttcga tccggatcaa
     4141 gcgggagttc gtgcagcact gcgaggcctt gatctgtttg tgaacagtgg gattggggtg
     4201 aaagtcgtca cattgccggc tggagaggac ccggatacct atgtgaggaa ggaagggtcg
     4261 gaggccttcg ctcgattgga agagcagtct ccgagcctct tagatttttc cctcgagcat
     4321 tgtttgagca cggcggagtc cggtacgatc gaagggcgta tccgcagtgt ggacgatgtg
     4381 ctgcggattc tgcaaaagag cgagcatcca atcgaacgag aggaacggat tcgcgtggtc
     4441 gccgaacgac tggggatcag ccaacaacga ttgatcgagc ggtatccggc attggtgcag
     4501 tcggagggaa gtcgaccggc gaccgttcaa tcggcggggg tcctaccggc gatgttcaag
     4561 ggtgcgagcg aagagcgaga tttgatgtat ttgctgctgc acggacatct gacaccggct
     4621 gatgtgcaac gactgcggcc cgaggccttt tccgttccag cctgccggtc cctcgtcgag
     4681 gcggcgctga atcacctcga tcgagacggc cgagtcgggc tcaggctatt gttggacgcg
     4741 gtgatcgatc atccggattg cgggtcgctg gcgacggagt tgtctatgcg ggaagaccat
     4801 ttcgacgatg tgaaggccca tgtcgcgggc tgtttagaga cgttggatcg gaaacgagcc
     4861 gaagccgtgt ttcgaaatct gatcgggcag ctcaaagccg cagaacgtga aggccgtgcg
     4921 gaggatgcgc aaaggttgaa cacacaagtg aatgaactgc gaatgcaaaa gtcggggcgg
     4981 ctctctatc
//
LOCUS       DATIPF010000133         5499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1351305, whole genome shotgun sequence.
ACCESSION   DATIPF010000133 DATIPF010000000
VERSION     DATIPF010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351305"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..718
                     /locus_tag="VLL06_03450"
     CDS             128..718
                     /locus_tag="VLL06_03450"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSS30052.1"
                     /translation="MDKLKDVLETYEFPLLLAAEWGQPERLLKLLDRSDTPQARKFKI
                     ISLLGEMQADEALPKLKEVLKDRDFAKQAIGAMGNLGREGIPLLVDIMNTSPQLELQA
                     AAAKGLGKLGGTHGDASVVPPLLTKLMDPKTDWSVLTEVAWSLGKIPDKRSIQPLYDL
                     DRKLETMRDPENVQLKKLKEAVFWAIKQCDTWDQFS"
     gene            complement(800..1420)
                     /locus_tag="VLL06_03455"
     CDS             complement(800..1420)
                     /locus_tag="VLL06_03455"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HSS30053.1"
                     /translation="MNRVSATLPFLFVGNHPCLDFINTELILDGRRTDLLEERADVIE
                     WLIRAKLLNANEGKGIAARLGQAEGVRLLSEAKAFRMVLREMAEQIVARKPVPQSVIA
                     TINRLLSQRPGYPQLLRTTGRVERHFYSSTQGPSQFLALLAEAASDLLCTKDLPLVKK
                     CRNQACILFFYDTTKNHARQWCSMSICGNRMKVAAHYRRQRRGAKT"
     gene            1618..1992
                     /locus_tag="VLL06_03460"
     CDS             1618..1992
                     /locus_tag="VLL06_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HSS30054.1"
                     /translation="MKTAIIILSDPKNGSEEALGRVFNALALASECQQKGDEVAVVFN
                     GAGTRWPAELTKLSHPANGLYNAVRDVVEGASCGCAEVFGAMEGVKSCGVPIVKDHAL
                     AGTAGLLSLRRYLAEGWNTIVF"
     gene            2069..2734
                     /locus_tag="VLL06_03465"
     CDS             2069..2734
                     /locus_tag="VLL06_03465"
                     /inference="COORDINATES: protein motif:HMM:NF015441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HSS30055.1"
                     /translation="MKVTQLWRYPVKSMGGEQLNHARISSLGIEGDRVVHVEDVHGHM
                     ITARTHHRLLSHHATLNASGEPLVDGLLWSDPTVSKAVAHIAGPGARLVRDESTNRFD
                     VLPLLVATDGAIAAFGHDGRRLRPNLVIGGVEGLAERDWPGQCLHIGDVIIGIQDLRG
                     RCVMTTFDPDSLKQDRQVLKDIARKFGGTLALNCYVIRGGEIQVGDPAELVPEHECEA
                     IRA"
     gene            complement(3081..4001)
                     /locus_tag="VLL06_03470"
     CDS             complement(3081..4001)
                     /locus_tag="VLL06_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSS30056.1"
                     /translation="MQTFKTAVLVTSVVVLAGCSSYIKQPEVCIDQKWYGYVQGPPCS
                     PTTKMAASDDTALRLAALEKERHRLADELAAAQRQNGALSSRVSDLERQLADRDREIA
                     ALRSGAGDSEKLAADLAASRQRNAELERRIADLEKQLADCEARLAGLRGQLDQSKDSL
                     AKAEKDLLKALQPEIAKGTVSVHQSGDALTINLASSLLFDSGQDLLKTGGTDALKRVG
                     GVLKDFPEKQVHVAGYTDNVAIKGPLQKKFPSNKELSDARANSAAQALRDGGVSSNLS
                     AAGHADSNPVASNDTATGRAKNRRVEVIVK"
     gene            complement(4090..4512)
                     /gene="mscL"
                     /locus_tag="VLL06_03475"
     CDS             complement(4090..4512)
                     /gene="mscL"
                     /locus_tag="VLL06_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HSS30057.1"
                     /translation="MLKEFKDFAMKGNVLDMAIGVIIGGAFGKIVSSLVSDVLMPPLG
                     LLLGKVDFSSLFLNLSGTPQPSLTAAKAAGAPTLNYGVFLQATFDFIIIAFVIFMLVK
                     QVNRFKREAPPAPPAPPPGPTNEEKLLMEIRDVLKGRH"
     gene            complement(4552..>5499)
                     /locus_tag="VLL06_03480"
     CDS             complement(4552..>5499)
                     /locus_tag="VLL06_03480"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HSS30058.1"
                     /translation="IEMSSGVLLIKTDAAADKTVKLNWANVEKLAINHPLPFHLKEGS
                     VLFGTATEGPNGTIKVQAEPLKETIQVPMESITALNPIIQAPVIYSGSLTGGYSQTTG
                     NSHLKNASLLGDFVARSEQLRLTLNGRYIYGDNANTLIARNARGTIKLDFFMTKRVFW
                     FVSAYFENDRFQDLKMRTALASGPGYQFVDRGEYGGLLKDMTLYAEAGLAYFNEDFRT
                     ANDTSSTRGRASIKLNWPLLDDKVTLYHFSEFYPSLQNTKNYYLTMDNGVRFKIFEGF
                     VSGFQVTTRLNSNPSPGTGDTDNLYLMTVGYSFDTTRKR"
BASE COUNT         1182 a         1448 c         1593 g         1276 t
ORIGIN      
        1 cgcctgtcag atgacctatc ttctcggtga catgaagatc aattgacaga aagaaatccg
       61 aaccgagttt gaagatgcgg gacaagccgc gcaatcggcc aacgcgggag atccggggat
      121 ctatgccatg gacaaattaa aggatgtgct cgagacatac gaatttccct tgttactggc
      181 cgctgaatgg ggtcagccgg agcgacttct gaagttgctc gatcgatccg atacgccgca
      241 agccagaaag ttcaaaatta tttctctctt aggcgagatg caagccgacg aggccttgcc
      301 gaaattgaaa gaggtgctca aggatcgaga ctttgcgaag caagcgattg gggcgatggg
      361 caatctcggc agagagggta ttccattgct ggtcgacatc atgaacacct ctccgcagct
      421 tgaattgcag gcagcggcag ccaagggctt gggaaaattg ggcgggacgc acggcgatgc
      481 ctccgtcgtg ccgccgttac tcaccaagct gatggacccc aagacagatt ggtccgtgct
      541 cacggaggtg gcgtggtcgc tgggcaagat tccggataaa cgatcgatcc aaccactcta
      601 cgatctcgac aggaagctag aaaccatgcg cgatccggag aatgtgcagt tgaagaaact
      661 caaagaagcg gtgttctggg cgatcaagca gtgcgatacg tgggatcagt tcagctaagc
      721 taagatgtac acctggtcgc gggatcacac cggcagccgc tcatttcctc aactccggtc
      781 catgcgtgac cggtgccagt tatgtcttcg cacctctgcg ctgtcttcgg tagtgcgcgg
      841 ccaccttcat ccggttaccg cagatactca tgctgcacca ctggcgggca tggttcttcg
      901 tcgtatcgta aaaaaataag atgcaggctt gattccggca cttctttacg agagggagat
      961 ctttggtgca cagcaggtcg ctggcggctt cggccaacag tgccaggaac tgagaggggc
     1021 cttgcgtcga agaatagaag tgacgttcaa cgcggccggt tgtacgaagg agttgtggat
     1081 agcctgggcg ttggctgagc aaccggttga ttgttgcgat cactgattgg ggaaccggtt
     1141 tgcgagccac gatctgttct gccatctcgc gtaggaccat ccgaaacgcc tttgcctcac
     1201 tcagcagtct cactccttca gcctgaccga gtcttgccgc tatccccttt ccttcgtttg
     1261 cattgagaag tttcgctctg atgagccact caattacatc tgcacgctcc tccagaagat
     1321 ccgttctccg cccatccaga atcagttctg tattgatgaa atccaaacac ggatgatttc
     1381 ccacaaacag gaatggcagt gtggcgctca cccgattcat cgataccccc cttcaatcga
     1441 tcatgacgaa accatctaat aggctcttga caggttagtc aaatttgtat ataaccgtca
     1501 aatacacatt tagacagtta caagctcaac ctccttttta aacggaatat cgacagtgcg
     1561 agtgttgctg aagcagccgc gtctccatca cacacaaatt tcccacaaag aggacgtatg
     1621 aaaactgcca ttattatctt gtccgatccg aaaaacggtt cggaggaggc gctcggacgc
     1681 gtgttcaacg ctctggcgct tgcatccgaa tgtcagcaga agggagacga ggtggccgtg
     1741 gtgtttaatg gtgccggtac acgatggccg gccgaactga caaaactttc acatccggcc
     1801 aatggcctct acaatgcggt tcgcgatgtc gtggagggtg cctcatgcgg ttgtgcggag
     1861 gtctttggag ctatggaggg ggtcaagtcc tgcggggtgc cgatcgtgaa agaccatgcg
     1921 ttggccggga cagcaggcct cttgagtctt cggcgatacc ttgcagaggg atggaatacg
     1981 atcgtctttt gagccgcact tcataaggcg gtcaggagac tctttcctga ccgtgttgat
     2041 ctgaatgact acgagggagg tcacgaccat gaaggtgacg cagctttggc ggtacccggt
     2101 aaagtcgatg ggtggtgaac agctgaatca tgcacgcatt agttcgctcg ggattgaagg
     2161 ggatcgcgtt gtccatgtcg aggacgtgca cggtcatatg atcacggcca gaacgcacca
     2221 tcggcttctc agtcaccatg ccacgctcaa tgcgtccggc gagcctttgg ttgacggcct
     2281 gctctggagc gatcctacgg tcagcaaagc tgtggcacat atcgcagggc ctggcgctag
     2341 gctggttcgc gatgagtcga ccaatcgctt cgatgtactg cctttgctgg ttgcgacaga
     2401 tggagccatc gccgcgtttg gacatgatgg ccggcgcctt agaccgaatc tcgtgatcgg
     2461 tggcgtcgag gggctggccg agcgcgactg gccgggccaa tgcctacaca tcggggacgt
     2521 catcatcgga atacaggatc tccgtgggcg ttgtgtcatg acgacatttg acccagacag
     2581 cctgaaacaa gaccgtcagg tgctcaagga catcgcgcga aaattcgggg gcacgcttgc
     2641 cttgaattgt tacgtgatcc ggggtggcga gattcaagtt ggagatcccg cagaattggt
     2701 accggaacat gagtgcgagg ccatccgggc atagggaatg agcgctgcca cccacctagc
     2761 gatgttcgcc gcaagaacct catcgatgac ggcctggtca gtggtgccgc accctgtcag
     2821 agcctatggg aatgagtagt gcctgattcc gacgagtaca atgggcggca acgctattgc
     2881 tcaagccatg acgattatgg gtctacccca tggaagcgtt cgggaaggaa ctccgagatt
     2941 ctggccagta tgtctggaga aacgcgcgac agaaatcggg caggccatct gttgcttctc
     3001 ggagggagga aggggctctt cccgtgggaa gagcccctct ttgcttcaaa cacaaatcga
     3061 atcattgctc tcttgtgccg ttacttgacg atcacttcca cgcggcggtt cttggcacgt
     3121 cccgttgccg tgtcgttgct ggcgaccggg ttgctgtccg catggcctgc ggccgataaa
     3181 ttgctgctca ccccgccatc ccgcagtgcc tgagcggcac tattggcacg agcgtccgag
     3241 agctccttgt tggatgggaa cttcttctgc aacggaccct taattgccac gttgtccgtg
     3301 tatcctgcca cgtgcacctg tttttcaggg aagtctttga gcacgccgcc cacacgcttc
     3361 aaggcatcgg tgccgccggt cttcaacagg tcttgccccg aatcgaacag caagctggag
     3421 gcgagattga tcgtgagtgc gtcgccggat tgatggaccg ataccgtccc tttggcaatt
     3481 tccggttgca gtgctttgag taggtccttt tcagccttcg cgaggctgtc cttgctctga
     3541 tcgagttgac cgcgcaggcc agccagacga gcctcgcaat ccgccagttg tttctcaaga
     3601 tccgcgattc gtcgttcgag ttccgcattc cgttgtctgg aagcagcgag atccgccgcc
     3661 aacttttccg aatcccctgc accggaacga agcgcggcaa tttcccgatc gcggtcggcg
     3721 agctgccgtt ccaaatcgct cacgcggcta ctgagcgcgc cgttctgcct ctgggcggcg
     3781 gccagttcgt cggctagccg gtgacgctct ttttcgagag ccgcaagacg gagggccgta
     3841 tcgtccgacg cagccatttt tgtcgtggga ctgcaaggag ggccttgcac atagccgtac
     3901 catttttggt cgatacatac ctcaggctgt ttgatgtagg aagaacagcc ggccagaacg
     3961 accacgcttg tcacgaggac ggcagtcttg aatgtctgca tgatgatttc ctccttctag
     4021 actgtgcggg ttatcgatcg tctaagtttt cctcccgcct tggaggaatg ctcgaggcac
     4081 caggggcgct tagtggcgac ctttcaagac gtcgcggatt tccatcaaga gcttttcttc
     4141 gttggtcggc cccggcggtg gcgctggagg tgccggaggt gcttctctct tgaatcggtt
     4201 gacctgcttg accaacatga agatgacgaa cgcgatgatg atgaagtcga atgtcgcctg
     4261 aagaaagacc ccgtagttca gggtcggcgc ccctgctgcc ttggccgcgg tgagagaggg
     4321 ttgtggcgtg ccagacagat tgagaaagag gctcgagaaa tcgaccttcc ccagtagcaa
     4381 cccgagcggg ggcatcagca catcgctcac gagcgacgag acgattttcc cgaaggctcc
     4441 gccgataatg acaccgatgg ccatgtcgag cacattcccc ttcatggcga agtctttaaa
     4501 ttctttcagc atagtttctc cttatgagga taagggtgta ttgcataggg actaccgttt
     4561 gcgggtcgtg tcgaaactat agcccacggt cataagatat aagttgtcag tatccccggt
     4621 gcccggcgac ggattgctgt taaggcgtgt tgtcacttga aagccgctca cgaagccctc
     4681 gaagatcttg aaccgcaccc cgttatccat cgtgaggtaa taattcttgg tgttttgcag
     4741 tgaaggatag aattcactga aatgatagag ggtgactttg tcgtcaagca gcggccagtt
     4801 gagtttgatg gatgcccggc cgcgagtcga ggaggtatcg ttggcggtcc gaaaatcttc
     4861 gttgaagtag gcaagaccgg cttccgcata gagcgtcata tccttcaaga ggccgccgta
     4921 ctcgcctcga tcgacaaatt gataaccggg gccgctcgcg agggcagtcc gcatttttag
     4981 atcctggaac cgatcgtttt caaagtaggc agacacgaac cagaacacgc gcttggtcat
     5041 aaaaaagtca agtttgattg tccctcgcgc gtttcgggca atgagggtgt ttgcgttatc
     5101 accatagatg tagcggccgt tgagcgttaa gcggagctgc tcactgcgag cgacgaagtc
     5161 tccgagcagg ctggcatttt tcagatggct gtttccggtt gtttgcgagt agccgccggt
     5221 caggctgcct gagtagatga ccggggcttg aatgatggga ttcagggcag tgattgattc
     5281 catgggcacc tgaatcgttt ctttcagcgg ttcagcctgc actttgatgg taccgtttgg
     5341 gccctcggtg gctgtgccga acagcactga cccttcttta agatgaaacg ggagagggtg
     5401 gttgatggcc agtttctcca cattggccca attgagcttg acagtcttgt ctgccgccgc
     5461 gtcagttttg atcagtagca ccccactgga catttcgat
//
LOCUS       DATIPF010000134         4125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_909186, whole genome shotgun sequence.
ACCESSION   DATIPF010000134 DATIPF010000000
VERSION     DATIPF010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909186"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VLL06_03485"
     CDS             <1..476
                     /locus_tag="VLL06_03485"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS30059.1"
                     /translation="FIRVTYQENRVERLLSRFNVFPFRCQLCTNRFRAFESGARHNTQ
                     AFDRRQYARLKASIDALIFDHKQLPVTNRITDISMDGCTLQMTGFPKGSFVELVLRPA
                     VEEDTIRIETAMVCSVRPLSMGIRFLEVPPEHYRHLAQVVLGLLVGQGIDSTLNP"
     gene            complement(466..1338)
                     /gene="panC"
                     /locus_tag="VLL06_03490"
     CDS             complement(466..1338)
                     /gene="panC"
                     /locus_tag="VLL06_03490"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HSS30060.1"
                     /translation="MKIIRTARSMGGWSERLRREGVIVGFVPTMGALHDGHRSLIRAA
                     RLRCDALVVSIFVNPAQFSLTEDLSTYPRPIAHDRALCREEGVDVCFEPTAQGMYPDG
                     FQTTVTVPALARRWEGEARPHHFAGVATVVTKLFGMVQPHLAWFGQKDYQQAVLVRTL
                     AEDLNLGVAIQVAQTVRERDGLAMSSRNIYLSPDERRIAPLLSQALRAGQQMIVGGTR
                     DVAAIERNMRQTIEQAPTIHVEYLAVCDQDNLEPLSVVPDRAVLLGAVRIGSIRLIDN
                     LLVNRLRGKSHARG"
     gene            complement(1335..1892)
                     /gene="folK"
                     /locus_tag="VLL06_03495"
     CDS             complement(1335..1892)
                     /gene="folK"
                     /locus_tag="VLL06_03495"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HSS30061.1"
                     /translation="MGRETVFIGFGSNVGDRVDFCDRAVTLLSLLPHSHVVGVSLLYE
                     TEPVNDHAHPGDGWFLNGVVQLETDVTPKSLLAILREIEHALGRDEDNRSGPRTMDLD
                     ILFYGQRVINEPDLVVPHPRMHQRRFVLIPLSELDPLFLHPSLQRTVNQLLAEVGKQP
                     EVRLLFPQPSTRYGSRPACSPSPGS"
     gene            complement(2176..3351)
                     /locus_tag="VLL06_03500"
     CDS             complement(2176..3351)
                     /locus_tag="VLL06_03500"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HSS30062.1"
                     /translation="MAGFPIEVATRIKTLPPYLFAAIDKMKQAALAKGVDIINLGIGD
                     PDLPTPTPIIESLALAAKDSKHHQYPSYEGMLAFRKAVADWYKRRFNVTLDPANEVLT
                     LIGSKEGIGHIHLAFVDPGDIVLVPSPGYPVYPVGTSFSGGVSHLMPLTKANGFLPDL
                     KAIPKDIAKKAKLMWLNSPNNPTSVIMSKDYFKRVVEFAQENQVIICHDAAYSEIYYD
                     GKRPTSFMEIDGAKDVGVEFHSLSKTYNMTGWRIGFVVGHKDVLAGLGKVKSNLDSGV
                     FEAVQAAGITALGLDDSVTDGIRKIYQARRDTLVPGLKSLGLDVELPPAAFYIWVTVP
                     KGYTSASFTAHLLETAGIVTTPGNGFGAPGEGYIRMTVCTSKERLAEAVERMKKAGW"
     gene            complement(3433..>4125)
                     /locus_tag="VLL06_03505"
     CDS             complement(3433..>4125)
                     /locus_tag="VLL06_03505"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HSS30063.1"
                     /translation="VRTMRAVCLTHVPFEGPGAFATALTARGVSLERYLVPQGGLPKD
                     TGDVLIVMGGPMSVNDPDSWIAEETAFIRSAILAGKPVIGVCLGSQFMAKALGATVRS
                     GKALEIGMTPITLTSEAKHDPVFSTLPESFDVFQWHGEVFDLPKDCVPLAGSDIAPLQ
                     AFRYSTRAYGLLFHLEMEANGIESLSRECASDLTNAGLTAQRIQSTALPHLPNLHKTA
                     DRLVAHLLNSAH"
BASE COUNT          866 a         1182 c         1178 g          899 t
ORIGIN      
        1 cattcattcg tgtgacctac caagagaaca gggtcgagcg cctgttgagt cgcttcaatg
       61 tgtttccgtt tcgatgccaa ttgtgcacca atcgttttcg tgcgttcgaa tccggtgcac
      121 gccataatac gcaggcgttt gatcggcgtc agtatgcccg cttgaaggcc tccatcgacg
      181 ccctgatctt cgaccacaag caattgccgg tcaccaaccg aatcaccgat atttcgatgg
      241 atggctgcac cctgcagatg acggggtttc cgaaagggtc tttcgtcgaa ttagtcttga
      301 ggccagccgt ggaggaggac acgatccgta ttgaaacggc gatggtgtgc tccgttcgcc
      361 cgttgtcgat ggggatccga ttcctggagg ttccgccaga acattaccgc catctggctc
      421 aggtcgtcct tgggcttcta gttggtcaag gcattgattc cacgctcaac ccctagcatg
      481 actctttcca cgcagcctat tcaccagcag attatcgatc aagcggattg acccgatccg
      541 gaccgcgccc aagagtactg cgcgatctgg gacgacggac agaggctcca gattgtcctg
      601 atcgcagacg gccaggtatt cgacatgaat ggtcggtgcc tgttcgatgg tctgccgcat
      661 gttccgctcg atcgccgcaa cgtcgcgcgt cccacctacg atcatctgct gacctgctcg
      721 gagcgcctga gagagcaagg gagcaatccg acgttcatcc gggctgagat agatattgcg
      781 agaactcatg gccagcccgt cccgctctct gactgtctgg gctacctgta tggccacacc
      841 gagattgaga tcctccgcaa gtgttcgaac cagtactgcc tgttgataat ctttctggcc
      901 gaaccaggcg agatggggtt gcaccattcc gaagagtttc gtcaccaccg tggccacgcc
      961 ggcgaagtga tggggtcggg cctcgccttc ccagcggcgc gcaagcgccg gcaccgtcac
     1021 ggttgtttgg aagccatcgg gatacatgcc ctgggccgtc ggttcaaaac agacatccac
     1081 tccttcctcg cggcagaggg cacgatcgtg ggcaatcggg cggggatatg tcgagaggtc
     1141 ttcggttagg ctgaattgtg ccgggttcac gaagatactt acgacgagtg catcacaacg
     1201 taatcgagcg gctcgaatga gggaacggtg gccgtcatgc aacgcaccca ttgtgggaac
     1261 gaatcccacg atcacaccct cccggcgtaa ccgttcgctc catccaccca tcgatcgtgc
     1321 ggtgcggata atcttcatga gcctggggac gggctgcagg cagggcgtga gccatagcgg
     1381 gtcgagggct ggggaaacag gaggcgaacc tcaggctgct ttccaacctc ggcgagcaat
     1441 tggttgaccg tgcgctggag agatggatgc agaaacagcg ggtcgagttc gctcaatgga
     1501 atgaggacga accgccgttg atgcatgcga ggatgcggca ccacgaggtc cggttcgttg
     1561 atcacccgtt ggccgtaaaa taatatgtca agatccatgg tcctgggacc cgatcgattg
     1621 tcctcatcac ggccgagcgc atgttcaatc tcccggagga tggccaagag gcttttcggc
     1681 gtgacatcgg tctcgagctg tacgacgccg ttcaagaacc agccatcgcc agggtgggca
     1741 tgatcgttca ctggttcggt ttcatagagc agcgacacgc ccacgacgtg tgagtgtggt
     1801 aacaggctca gcaacgtcac tgcacggtca cagaaatcga cccgatctcc gacgttcgac
     1861 ccaaacccga tgaacaccgt ttctctaccc aactttccct cttctgccgt ctatctatat
     1921 ggtggccgag ctgggcctaa ctgcgcgcgt ccattctcac ccgcccaccc acgggcacgt
     1981 cgagacgtgc ccgtgggtgg gcgagggcct tcagattcct caactttccc agaggaagca
     2041 ggcggattgt tcttcactgc gcgcattgag cgaccaccga tttatcgtgg gggctctgcg
     2101 agcaagaaga acggtctgcc tgcttcctca catccttctg aggccgcgcg ttacgcgagc
     2161 acgaggcaca ctcggctacc aacccgcctt cttcattcgc tccacggctt ctgccagtct
     2221 ttctttcgac gtacacacgg tcatcctgat atagccttca ccaggcgcgc cgaatccatt
     2281 gcccggtgtc gtcacgatcc cggctgtctc caacaagtgg gccgtgaacg atgcggacgt
     2341 gtaccccttc ggtactgtga cccagatgta gaatgcggca ggcggcagct cgacgtcgag
     2401 gccgagggat ttcaaccccg gtaccaacgt gtcgcgccgt gcttgataga tcttccgaat
     2461 accgtctgtt acggaatcat ccagccccag tgccgtgatc ccggcagcct gcaccgcttc
     2521 gaacacgccg gagtccaggt tactcttcac cttgccgagc ccagccaaca catccttgtg
     2581 gccgacgaca aagccgatac gccagccggt catgttgtac gttttcgaga gcgaatggaa
     2641 ttccaccccg acgtccttgg ctccgtcgat ttccatgaag ctcgtggggc gtttcccgtc
     2701 gtagtagatc tccgaatagg ccgcgtcgtg acagatgatg acctggttct cctgcgcaaa
     2761 ttcgacgacg cgcttaaaat agtccttgct catgatgacc gacgtcgggt tgtttgggga
     2821 attcagccac attaacttcg ctttttttgc gatgtctttc gggatcgcct tcagatcggg
     2881 caagaatccg ttcgccttcg tcaacggcat gaggtgtgac acgccgcctg agaaactggt
     2941 gccgaccgga tacacgggat acccagggct cgggacgagc acgatatcgc cgggatcgac
     3001 gaacgcaaga tgaatgtgac cgatcccttc ctttgacccg atcaatgtga gcacttcatt
     3061 tgccggatcg agcgtcacat tgaatcggcg cttgtaccaa tcggctacgg ctttgcgaaa
     3121 cgccagcatg ccctcgtagg aaggatactg gtggtgcttg gagtcctttg ccgcgagtgc
     3181 cagactctcg ataatcgggg ttggagtcgg cagatcggga tcaccgatgc ccagattgat
     3241 gatgtcaacg cccttcgcaa gagcagcctg tttcatctta tcgatggcgg cgaacagata
     3301 gggaggcaag gtcttaattc gagtggcaac ttcaataggg aaaccggcca tgtcagagac
     3361 tctccttttc tagatgaggt actatggagg tgcaatcgaa ggttctaggc tacttcagga
     3421 gggcagaggt aatcaatgcg cagaattgag cagatgagca accagccgat cggcggtctt
     3481 atggaggttc ggaagatggg gaagtgcggt tgattgtatc cgctgtgcag tgagacctgc
     3541 gttcgtgaga tccgatgcac actctcggga caacgattca attccgttcg cttccatttc
     3601 caagtgaaac aagagcccat aggcacgagt ggagtagcgg aaggcttgca ggggagcgat
     3661 gtcggaaccg gccaagggca cacagtcttt cgggaggtcg aacacttcgc cgtgccattg
     3721 gaagacgtca aaagattcag gtagtgtgct gaatacaggg tcgtgttttg cctcagacgt
     3781 cagcgtgatt ggcgtcatac cgatttccag tgccttccca gaacgtacgg tggcacccaa
     3841 cgcctttgcc ataaattgac tgccaagaca gacaccgatg accggcttgc cggctaggat
     3901 ggcggatcga atgaatgctg tctcctcggc aatccaggaa tcgggatcgt tcaccgacat
     3961 cggtccaccc atcacgatca gcacatcgcc ggtatctttg ggcagcccgc cttgtggcac
     4021 gagatagcgt tccaggctta cgccgcgcgc agtcagcgct gtcgcgaagg ctcccggccc
     4081 ttcaaaggga acatgtgtca gacagactgc gcgcattgtc cgtac
//
LOCUS       DATIPF010000135         4450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_291844, whole genome shotgun sequence.
ACCESSION   DATIPF010000135 DATIPF010000000
VERSION     DATIPF010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291844"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VLL06_03510"
     CDS             complement(<1..888)
                     /locus_tag="VLL06_03510"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HSS30064.1"
                     /translation="MTKTQKPRRAAPTTKKGTRASLVTAAELLLEIGVEELPYQFIAP
                     ALAALKDSAEQLFKDQRLAFQSARTLGTPRRLTLVVDGLATKQTSMVKETMGPSKAVA
                     FDPAGQPTRAATGFAAGQGVSVQDLEIRRTPKGEYLYAVKQEQGQPANVVLKELLPQL
                     IAKLSFPKAMKWNQAGVRFARPVRWLVVLFGGATLPITAAGITAGNRTEGHRVLGSAK
                     GIAVRDAESYLKGLERQGVIPDPARRRHMIEEQIATLCKKAGFTLNVDGDLLDQAVYT
                     TECPNAIIGSFKPAYLEVPQ"
     gene            complement(905..1846)
                     /locus_tag="VLL06_03515"
     CDS             complement(905..1846)
                     /locus_tag="VLL06_03515"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HSS30065.1"
                     /translation="MTFQDLILTLHHYWAEQGCVIHQPYDLEMGAGTFHPATFLRSLG
                     PEPWRAAYPQPCRRPTDGRYGENPNRMQHYYQYQVVLKPAPDNIQSLYLESLAQLGIN
                     PKQHDIRFIQDDWESPTLGAWGLGWEVRLDGMEITQFTYFQEIGGIELNPITVELTYG
                     TERIAMYLQQVNNVFDLAWNNSVTYGDIHHETEVQFSTYNFEEGDVAMLMATFQSFEG
                     ECKRLLAHRDKRLTLPAYEFCIKSSHLFNLLDARGAISVAERTGYIARVRALARQCAE
                     RYIEERAAMGHPLLNRETEREGATLSSIRSKKVARRS"
     gene            2125..2559
                     /locus_tag="VLL06_03520"
     CDS             2125..2559
                     /locus_tag="VLL06_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSS30066.1"
                     /translation="MKLSKKSEYGLRALLELTLVHGKMTLQRHQIATRQHIPVEFLEQ
                     ILLTLKRAGLLSSRRGMKGGYTLIKPPEEITLGQVIRILDGPLAPIGCVSKTAYQKCK
                     DCPYAEHAQCPVQHVMGTVRDAIADILDHYTLNDFVSGHRKG"
     gene            2574..3395
                     /locus_tag="VLL06_03525"
     CDS             2574..3395
                     /locus_tag="VLL06_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSS30067.1"
                     /translation="MLVVITFVAATVNGALGYGFSSITVPVALLFYTNRILNPALVLV
                     ELMINGYVLFINRKSIPNIWWRVAPILIGLLIGVAIGSYILSLVHPSWVKFVTYFMLL
                     PLILLQAAGIRKPIRAEKAIAIPFGVGIGTLYSVTTISGPPLALLFNNQGYVKQDFRA
                     ALGVIRVGEAVMTGIAYYFIGLYSYASMEIILYIVPSVLLGIPLGSYLIRWMDPETFR
                     RICMAFDALVVAFGLSRVLIELNLATAFTTYSILTIMMMINAYLLYRFFRQRTVP"
     gene            3855..4295
                     /locus_tag="VLL06_03530"
     CDS             3855..4295
                     /locus_tag="VLL06_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HSS30068.1"
                     /translation="MSDSTNPPVFIPQTRAMMTTGRPRCLTAIVAGLLLTTMSFLTGC
                     GGGGETGSDPTVKVTPGAGATASLDWNPVPDPSVSAYFVHYGRQSPHQPGSCAYEHSV
                     SVDSPSATLTDLVPDTHYYFAVSAYNGLESACSNEVSTVTPPTV"
BASE COUNT          986 a         1270 c         1120 g         1074 t
ORIGIN      
        1 ttgcggtacc tccaggtagg ccggcttgaa tgatccgata atggcattgg gacattcggt
       61 cgtatagacc gcctgatcca gcaagtctcc gtccacattc aacgtgaaac cggccttctt
      121 gcacaacgtc gcaatctgtt cttcgatcat atggcgacgg cgcgccggat ctggaatgac
      181 gccttgacgt tcaagcccct tcagatagga ctcggcatct cgaaccgcaa tccccttcgc
      241 acttcctaat acgcggtggc cttctgttcg attaccggcg gtaattcccg ccgctgtgat
      301 gggaagtgtg gcccctccga acagcaccac cagccaccgt accggccgag cgaatcgcac
      361 cccggcctga ttccacttca tcgctttcgg aaacgagagc ttcgcgatca gctgcggaag
      421 gagttctttc aagacgacgt tggcgggctg cccttgttcc tgctttacgg catagagata
      481 ctcccccttc ggagtccggc gaatctccag atcttggacg gagactcctt gccctgcggc
      541 aaaccccgtc gcggctctgg tcggctggcc ggcagggtcg aacgccaccg ctttggaagg
      601 ccccatcgtt tccttgacca tagaggtttg cttggtcgcg agaccatcaa ccaccagagt
      661 caatcgccta ggagttccca acgtacgcgc ggattgaaac gcgagacgtt ggtctttgaa
      721 caactgctcg gccgaatctt taagggcagc caaggcggga gcgatgaact ggtagggaag
      781 ttcttcgacg ccgatctcaa gcaacaactc ggcagcggtc actagagaag cgcgagtccc
      841 ctttttcgta gtaggagcag cacggcgagg tttttgagtc tttgtcatgg atcatcccga
      901 ggttctagga gcggcgggcc accttcttgg aacggatact tgataacgtt gctccttcgc
      961 gctccgtctc gcgattgagc agcggatgtc ccatcgccgc gcgctcctcg atgtagcgct
     1021 cggcacattg acgggcaagg gcgcggaccc tggcaatgta tcccgtgcgc tctgccacgc
     1081 tgatcgcccc gcgggcatcg agcagattga agaggtgcga agacttgata caaaattcat
     1141 aggccgggag tgtcagtcgc ttgtcgcgat gcgccagcag ccgcttgcat tctccttcga
     1201 acgattgaaa cgtggccatg agcattgcca cgtcgccttc ttcaaaatta taggtcgaga
     1261 actggacctc ggtttcgtga tggatatccc cataggtgac tgaattgttc caggcaaggt
     1321 cgaagacatt atttacctgt tgaagataca tcgcgatacg ctcagtcccg tatgtaagtt
     1381 cgacggtaat gggattgagc tcgatcccgc cgatttcctg gaaatacgtg aactgggtaa
     1441 tctccattcc atcaagacgc acctcccagc ccaggcccca ggcacccagc gtgggggact
     1501 cccagtcgtc ttggataaac cggatgtcgt gctgtttcgg gttaatcccc aattgagcca
     1561 gactttccaa atacaacgat tgaatgttgt ccggcgccgg cttcagcacg acctgatact
     1621 gataatagtg ctgcatgcga ttcggattct cgccgtatcg cccatccgtt ggacgccggc
     1681 atggttgggg ataggcggcg cgccagggct ctgggccaag ggagcggagg aacgtggctg
     1741 gatgaaaggt acctgccccc atctctagat cgtagggctg atgaatgacg cagccttgct
     1801 cggcccagta gtgatgcaat gtgagaatga gatcctggaa ggtcacgaac agtccagtcg
     1861 tggttgcgga ggtctgaatt ctcgcgacag acgcgttgaa atgaacggag aagctaacag
     1921 gaatgcgcag aatctgtcaa ggaacaaccc ctctaggatc aaggagttgc agcgcatccg
     1981 ctcataaaaa tggacggacg ctgtggatca gactcttcga agtattccgc tcaaatactt
     2041 caaagcagct cttgacgaga ccagacgcag tcgactattg tcaccattct tgattaaatc
     2101 actcggaatt taattagacc tgccatgaaa ctctcgaaga agagtgagta cggacttcgt
     2161 gccctgctcg agctcaccct ggtgcacggc aagatgacat tgcagcgcca ccagattgca
     2221 acacgccagc atatcccggt cgaatttctt gaacagatcc tgctgaccct gaaacgagcc
     2281 ggcctccttt cgagtcgtcg aggaatgaag gggggctaca cactgattaa gcctccggaa
     2341 gagattaccc tcggccaagt cattcgtatt ttggatggcc cgctcgctcc catcggatgc
     2401 gtgagcaaga cggcctatca gaaatgcaaa gactgcccct atgctgaaca tgcccaatgt
     2461 ccggtgcagc atgtgatggg tacggtgcgt gacgccattg ccgatattct ggaccactat
     2521 acgctgaacg acttcgtgtc cggacatcgc aaagggtaat cgtggatctc gtcgtgcttg
     2581 tggtaatcac gttcgtggcg gccacagtca acggcgcctt aggatacggc ttttcctcga
     2641 ttacggttcc agtcgcgctg cttttttata ccaatcgaat tctaaatccg gcattagtgc
     2701 tcgtggagtt gatgatcaat ggctacgtcc tctttattaa ccgcaagagt atcccgaata
     2761 tttggtggcg cgtcgcaccc attctgatcg gtctcctgat cggtgtcgcg atcggcagct
     2821 acattctctc tctcgttcat ccatcctggg tcaagtttgt gacctacttc atgctgttgc
     2881 cattgattct gctccaagct gctggtatca gaaaacccat ccgagccgaa aaagccatcg
     2941 cgatcccctt cggggtggga attggaaccc tgtactccgt caccaccatt tcaggccctc
     3001 cccttgcgct cctgtttaac aatcaaggat atgtcaaaca ggatttccgg gccgcactcg
     3061 gcgtcattcg agtgggagag gcggtcatga caggaatcgc gtactatttt attggactct
     3121 acagttacgc cagtatggag atcattctct atatcgtccc cagcgttctg ttgggaattc
     3181 cgctcgggag ctatctcatt cgttggatgg accctgaaac gttcaggcgg atctgcatgg
     3241 ccttcgatgc cttagtggtc gcatttggat tgtccagggt acttatcgaa ttgaatctgg
     3301 ccaccgcatt caccacctac agtattctca cgatcatgat gatgatcaat gcctacttac
     3361 tctatcgatt ttttcgccag agaaccgtcc cctagggagc gtggaaggaa taccggccaa
     3421 tcaattaccg tgactggctg aatcaagttt ttaccgcctc tctcttatct ctctaaggtc
     3481 cccggtcgcc cacttcctca aacaggtccc tttctccttt tgtcgtttga accctcgacg
     3541 ttcgcagaat atctatgatc acattctcat acccattcat ggacactcac tgatgaatga
     3601 gccagcgcaa atgggataac gctcctttgg caggataaaa tctgtatggt ctctccttca
     3661 cttcctacac ctcacacatt ctaaaaacac cgcacaagac cttaattcac ttgataaatt
     3721 agcaacttgc aagggaaaca tgtggcacat gctttgctca tacgcgaagc atgtttgttg
     3781 tctcattcag cgtcacacgt gaagtagatc gaacctaaat catcgcggcc taaccatttg
     3841 cgggagagca gcccatgagc gattcgacca acccacctgt ctttataccg cagaccagag
     3901 ccatgatgac caccggcagg ccgcgatgcc tgactgccat cgttgcaggt cttcttctta
     3961 caaccatgtc ttttctgaca ggatgcggag gcggagggga gaccggcagc gaccccaccg
     4021 tcaaagtgac tcccggtgca ggcgccaccg catctctcga ctggaacccg gttccagatc
     4081 cctcggtctc tgcctatttc gtccattacg gccggcaatc accgcatcaa ccgggtagct
     4141 gcgcgtacga acattctgtt tctgttgact ctccatcagc cacgcttaca gaccttgttc
     4201 ctgataccca ctattatttt gcggtcagtg cctataacgg tctcgagagt gcctgctcaa
     4261 atgaagtatc gaccgtcacg cctcctaccg tctagattga tcctctaaga ccctcctaac
     4321 acacttccgc tgtcaacgac attggatccg cctggatccc gcccccaatg tcgtgaccga
     4381 acagttattt atggtcaaca tctgcacgtc atccttgcag cccttctgtg gccatactgg
     4441 gccagtgtga
//
LOCUS       DATIPF010000136         3527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1262067, whole genome shotgun sequence.
ACCESSION   DATIPF010000136 DATIPF010000000
VERSION     DATIPF010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262067"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VLL06_03535"
     CDS             complement(<1..717)
                     /locus_tag="VLL06_03535"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30069.1"
                     /translation="MDTAGLHDSELGELQRRLVILRVGLLLVVALLGLRLWHLQIREG
                     PYYRDLSENNRTRSVVMEPARGLIYDRHGVLLANNVPSFTLYVSLEDVKDRDLLIDQL
                     STLLGLDAALVRTKLTARGSKQLPRKVKGRLTLREATLIESHRLDLPGVMVQVESQRN
                     YPGGVMASHLLGYVGEVSPEQLDKPEFADLHQGSIVGQYGVEKFFDQLMRGQAGQKSI
                     EVDAVGHEKRTAVVEQPHAGD"
     gene            complement(758..1249)
                     /locus_tag="VLL06_03540"
     CDS             complement(758..1249)
                     /locus_tag="VLL06_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30070.1"
                     /translation="MKFLIYLMLVLVLVPLQTTLLPHLSVWNIKPDLGLVAAAFVGIF
                     AGELEGLLVGLAIGWVLNMFSAGELWISLLTNGGVGVLAGFLGRQVSQMTPVTVGVGL
                     LLVSLVSGIFAAMNFPHLDASQMWWMIESIVFPQACFDGVVTAGLYWLLSQRFDVARL
                     RTE"
     gene            complement(1246..2121)
                     /gene="mreC"
                     /locus_tag="VLL06_03545"
     CDS             complement(1246..2121)
                     /gene="mreC"
                     /locus_tag="VLL06_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HSS30071.1"
                     /translation="MAHSRTPYGARRLALACFALLVVALFLLPNQIQGLLQYLDGPVG
                     HVVRVPLEAIASIDAGVADRWAQYVALQGVREENQQLRKEIEWLRGQHNQLRESAAAT
                     ERLTGLLSFKEQALPTMVAAQVIGRDATNRYRAIILNKGESDGIKPDMGVVTPAGVVG
                     RVVKTTGASSVVLLVTDPNNAIAGLIQRTRDEGVVEGTQEGLARLKYIPLLSTLRDGD
                     RVVTSGLVGGFPRGLVIGTITRIDKEEGALFQSAELMPEVDVSRVEEVLVIQTSYGQP
                     TKAGAGADLLGKPAP"
     gene            complement(2150..3199)
                     /locus_tag="VLL06_03550"
     CDS             complement(2150..3199)
                     /locus_tag="VLL06_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HSS30072.1"
                     /translation="MGFGSDIFGWFSNDLAIDLGTASTLVYVHGKGIVLNEPSVVAVE
                     KRSGRVLAVGTEAKKMLGRTPGNIIAVRPMKEGVIADFEMAEQMLKRFIQKAHNRSAF
                     VRPRIIIGVPSRITQVEQRAVRDSAELAGAREVYLIEEPVAAAIGAGLPITEPSGNMV
                     VDIGGGTTDIAVISLGGIVYSESVKVAGDRMDDAIMNYIKKKYNLLIGEHMAERIKFE
                     IGSAYPFEERKTMMIKGRDLISGIPRTLVVDDAEIREALQEPIGTIVNAIKVALENTP
                     PELAGDIIDRGVVLTGGGSLLKGMDTRFREETNLPIITVDDPLTSVVLGVGKILDELD
                     LLAKVSVMSQANTYR"
     gene            complement(3236..>3527)
                     /locus_tag="VLL06_03555"
     CDS             complement(3236..>3527)
                     /locus_tag="VLL06_03555"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HSS30073.1"
                     /translation="ADGFAGGRSIGKRLIGLQTVLPDRRTAAGFRESIIRNLPFAVAQ
                     LAFAVPYVGWLVSVAIIVFEAVLIIGNEQGRRLGDEVAGTQVLDAGRLALPD"
BASE COUNT          806 a         1126 c          876 g          719 t
ORIGIN      
        1 gtctcccgca tggggctgtt ccacaaccgc ggttcgtttc tcgtgaccaa ccgcatcgac
       61 ttctatgctt ttttgcccag cctgaccgcg catcagctga tcgaagaatt tctccacacc
      121 atattgtcca acaatgctgc cctggtgcag gtcggcaaat tcaggtttat ctaattgctc
      181 gggcgacact tcccccacat agcccaacag atgcgaggcc atcaccccgc caggatagtt
      241 ccgttgcgat tcgacttgga ccatgactcc gggcaagtcc aggcggtgag attcgatcaa
      301 cgtggcttcc cgaagagtta agcgaccctt gaccttgcgc ggtaattgtt tgctgccccg
      361 agccgtgagc ttggtcctga cgagcgcggc atcgagccct aagagagtac tgagctgatc
      421 gataagcagg tcccgatctt tgacgtcctc aagtgagaca taaagcgtga agctcggaac
      481 attattcgcc aacagcaccc cgtggcgatc gtagatgagt cctcgagccg gctccatgac
      541 gacagaccgc gtccgattgt tctcggagag atcgcggtag tagggccctt ctcgaatctg
      601 taagtgccaa agccgcaacc ccagcaatgc cacgacgagc agcaagccga cgcggagaat
      661 gaccaatcgg cgttgaagct ctccaagctc agaatcgtgg agtccagctg tatccatgcg
      721 tcacaaccaa tatttcgcca cacccctcac agacctgcta ttcggtcctc aggcgggcga
      781 cgtcaaaccg ctgagagagc aaccaataga gccccgccgt cacgactcca tcaaaacacg
      841 cttgtggaaa gacgatcgac tcgatcatcc accacatctg agacgcatcg agatgtggga
      901 agttcatcgc ggcaaatatc ccgctcacca aagacaccag caacagtccc acacccacgg
      961 taaccggcgt catctgcgat acctgacgtc ccaggaagcc cgccaagacc ccgacccccc
     1021 cgttcgtgag gagactgatc cacagctcgc cggctgagaa catattcagg acccatccga
     1081 tcgcaagccc cacgagaagg ccttccagtt cgccggcaaa tattccgacg aacgcggcgg
     1141 ctacaagccc caaatccggt ttgatgttcc acacactcag atgtggcagt agcgtcgtct
     1201 gcagggggac cagcaccagc acgagcatca gatagataag gaatttcatg gtgccggctt
     1261 ccccagcaga tccgctcctg ccccggcctt cgtcggctgt ccatacgacg tctgaatcac
     1321 caacacctcc tccacacggc tgacatcaac ttccggcatg agttccgcag actggaagag
     1381 ggcaccttct tctttatcga tgcgcgtaat cgtgccgatc acaagccctc gagggaaccc
     1441 tccgacaagg cccgaggtca cgacccgatc accgtctcga agggtcgaca gcaacggaat
     1501 atacttgagc cgagccagtc cttcttgagt cccttccaca acaccttcgt ctctcgtccg
     1561 ctgaatgagg ccagcgatgg cattgttcgg atcggtcacc aagagcacca ccgaagacgc
     1621 gcccgtcgtt tttacgacgc gcccgacaac cccagcgggc gtgactactc ccatatccgg
     1681 cttgatccca tcactctcac ccttattgag aatgatggca cgatagcggt tcgtcgcatc
     1741 acgtccgata acttgagcgg cgaccattgt cggaagcgcc tgttccttaa agctcagcaa
     1801 acccgtcagt cgctctgtcg cagcggcaga ctcacgcaac tggttatgtt gcccacgcaa
     1861 ccactctatt tctttccgga gttgttggtt ctcctcccgt accccttgca acgcgacata
     1921 ctgtgcccat cgatcagcca cgccggcatc gatggacgca atggcttcga gcggaacacg
     1981 cacgacgtgt ccaaccggcc catccagata ctgcaggagt ccttggattt gattgggaag
     2041 caggaaaaga gccaccacca ggagggcgaa acaggcaagt gccagccttc tggcaccata
     2101 gggagtacga gagtgagcca tccacataaa aggagttagg gatggaacct tatcggtagg
     2161 tattggcctg ggacatcacc gacactttgg cgagcagatc caactcatcg aggattttcc
     2221 cgacccccag gacgacggac gtcaggggat catccacggt gatgatcggc agatttgtct
     2281 cctcccgaaa gcgcgtatcc atgcccttca gcaaagaccc cccgcccgtg agtacgacgc
     2341 cacgatcgat aatatcaccc gccaattcag gcggagtatt ttccagtgcc actttaatcg
     2401 cgttgacgat tgtcccgatc ggttcctgca gcgcttcacg gatttctgcg tcatccacca
     2461 ccaacgtacg gggaatgccg gaaatcaagt cacgcccctt gatcatcatg gtcttgcgtt
     2521 cctcaaaggg gtaggcggac ccgatttcaa atttgatccg ttccgccata tgttcaccga
     2581 tgagaaggtt atactttttc ttaatatagt tcatgatcgc atcatccata cgatctcccg
     2641 cgaccttcac ggactcgcta tagacgattc cgccaagtga gatgaccgcg atatcggtcg
     2701 tgccaccgcc gatatcgacc accatgttgc cggagggttc ggtgatcgga agaccggcgc
     2761 caatggctgc ggccactggt tcttcgatca gatacacttc gcgggcaccg gccaattcgg
     2821 ccgagtctcg tacggcccgc tgctccacct gcgtaatgcg ggacggcaca ccgatgatga
     2881 tacgcggacg cacgaatgcg ctacggttgt gcgctttctg aatgaaccgt ttcaacatct
     2941 gctctgccat ttcgaagtcg gcaatgacac cttctttcat gggccgcacc gcaatgatgt
     3001 tgcccggcgt gcgccccaac atcttctttg cttccgtgcc gaccgcgagc actcgtccgg
     3061 accttttctc aaccgccaca accgagggtt cgttgagtac gatccctttc ccatgaacat
     3121 atacaagggt cgacgctgtc cccaggtcaa tggccagatc gtttgagaac catccgaaga
     3181 tatcgctccc aaagcccacg gttgctctcc cttctcctta gacgctgatg gcgcctcagt
     3241 cgggcaacgc tagtcgaccc gcatccaaca cctgggtgcc ggccacctca tcaccgaggc
     3301 gtcgtccctg ttcgttccca ataatcaata ccgcttcgaa cacaatgatg gcgaccgaca
     3361 caagccaccc aacatagggg acagcgaagg ctaactgagc gaccgcaaag ggcagattgc
     3421 ggattatgga ttcccgaaac cctgctgccg tgcgtcggtc cggaagcacc gtctggagac
     3481 cgatcagccg cttcccaata ctgcgtccac cggcaaatcc atcagca
//
LOCUS       DATIPF010000137         9161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_645375, whole genome shotgun sequence.
ACCESSION   DATIPF010000137 DATIPF010000000
VERSION     DATIPF010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9161
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645375"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VLL06_03560"
     CDS             <1..713
                     /locus_tag="VLL06_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSS30074.1"
                     /translation="AILYPALMMIVGISVLFFLMTFVVPKITAVFTSLKQALPWPTVV
                     LMGISNFLADYWAVILGGVVLMVWAVRRAMKTETGQLTADRWLLKIPLIGEVSRMVAI
                     SRLTSTLATMLASGVQLLDAMDVAKRVMNNRVLEHAVEGARQNIREGETIAEPLKRSG
                     EFPALVTHMIAVGERSGEMEEMLRRIGHIYDGEVDRVITRFTSLLEPIMILVMGVLVF
                     FIVVAILLPIFEMGQMVR"
     gene            738..1238
                     /gene="gspG"
                     /locus_tag="VLL06_03565"
     CDS             738..1238
                     /gene="gspG"
                     /locus_tag="VLL06_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HSS30075.1"
                     /translation="MGQDRHTHERTVELELRSDRGFTFIEIMVVVAILAILAALVIPR
                     IMGRTDDAKRTAAKVQIRNIEGALQLYKLDNGVYPSTEQGLKALVEKPTVGVIPKKWK
                     IGGYLPKLPEDPWQNPYKYLSPSQKRTPSGTPLGDYELMSLATDGEVGGEGINADISN
                     FNLDKD"
     gene            1246..1866
                     /locus_tag="VLL06_03570"
     CDS             1246..1866
                     /locus_tag="VLL06_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30076.1"
                     /translation="MVIGYSSVAGHAQTGSAHRAFPALHSSRGFTILEIILVLFLLVV
                     LLGIILPRISLEENLGSVGRQMVGTIRSLQSLAMSTQKTVHLYVDIERGVYWPMIIDG
                     SQEKLPTDPTWAMPLKLPDTIRISDMWVDQGKKESGRVDLFFYPTGRIDPATMHLVNT
                     NNAILAIAIEPVTGAIRMSDERIEPPKPFIIPDRVRPLLKIAVAGP"
     gene            1911..2324
                     /locus_tag="VLL06_03575"
     CDS             1911..2324
                     /locus_tag="VLL06_03575"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSS30077.1"
                     /translation="MGNERGFTLLEVVIALGILALALPILLGLRNWDLDLHARAKEMT
                     AATILAQEKLIETELGTLLPLGETSGEFLPTPLGSQPTTVVTNRQSNYRWKRIVSSTP
                     LPIVREVKIQVLWPRGTIDEMLEVSTYVFSTTPTP"
     gene            2296..2916
                     /locus_tag="VLL06_03580"
     CDS             2296..2916
                     /locus_tag="VLL06_03580"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HSS30078.1"
                     /translation="MSFRQPPRLEGGFTLVEVLLAVSLVAMMAALVFGSLFVTTSAID
                     AARASSANEQIIRSTMRVMIDELSVGKSSQAGPWMGINGQFDGQPADTIAFLTMGQFR
                     GAESTKDTEVVRIVYTREGDRLLRYVRKNLYGLTDESVGQIELATKVKAFNVRYYDEK
                     NRLWVDEWDGRGRLQSAPKAILIELTLLQENAELQTIRQWVTVGAS"
     gene            2913..3809
                     /gene="gspN"
                     /locus_tag="VLL06_03585"
     CDS             2913..3809
                     /gene="gspN"
                     /locus_tag="VLL06_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HSS30079.1"
                     /translation="MIKWSWLETLPLKGPLAWILASLACFIIFLFLTFPYGPLQNRLL
                     TEVNRATGWDVRATDWSVGFPAGIEWHDVVLAGPPMGAISIKDVRATIGVFEAILGQL
                     VLDYAVQLPGGTQGGAGRATGSLTTASWQLRGPVAVKGHLQQMDLATVLKPYVTSGMI
                     QGDFTHRWDGAQGSNAALKGEGTVKVEIKDFVIDQIPSGTQSMPSLTFGRIQAAFTCR
                     DAACDVTELKGDGVDGSFSVQGHVTLQHPLQQSLLDLTVTVVPGAGFAQKAASFSLPP
                     FQPGTQLTFKLVGPIMNARVAL"
     gene            3926..5500
                     /gene="pilM"
                     /locus_tag="VLL06_03590"
     CDS             3926..5500
                     /gene="pilM"
                     /locus_tag="VLL06_03590"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HSS30080.1"
                     /translation="MIAECVGLDIGQTAIKAVRFRRRLTGRESVEYFHLPVPYGRPEQ
                     TEPAYRAAMLRNFLWQHGLYGSGEIVTALPCQDVFVRTLSFPFRDAAKLAQVVPFEME
                     NLIPMPLEDVTVGSLLLPARETQEGDSKAKGSEVLVTAAPKDKIAEHLQFMASADLNP
                     SAITIDGMALFSVSQFLKEEGAQVPGDMAIIDVGASKTTLCLIHEGRPVLLRTVLWGG
                     NHLTHALAVRYACSFAEAERRKRAMAVEEVDTWLEPVLKELRVSLHSYEGTVHQRLSH
                     CWVSGGGSKLKELSGHMARQLGLLPVGPRQGFGAGCPRAFSIAFGLAIHPNIVRPRWK
                     SRLAGSSLALDFKASSDAASAAGHVSKQDRRLALWGALILGILALSDLSVRVMLKDST
                     VKNLKGEIQAQYEQTFGPGANAGEELDQARYRVSQVEKGLSVVDGTRYNVLASLAELV
                     KHVPPGTPLTIRELTIDGTSIHLEGETTTFDTVEKIKQAFVATDSFQDVTISDTRVGA
                     VQNQVVFRLTYTVQRP"
     gene            5497..6045
                     /gene="gspM"
                     /locus_tag="VLL06_03595"
     CDS             5497..6045
                     /gene="gspM"
                     /locus_tag="VLL06_03595"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HSS30081.1"
                     /translation="MMQMLKERWNHFSQRERIIVATGMTALAAALVFLLIIDPLMDTI
                     DKLDRQARRKAKDSQELMIVAQEYVAKQARIAKLEERMPNPPAQFSLLAFMEEATTTA
                     QIRDRIGGMQPHAPMVVQGYQETAVDLRLDGVSLPQILALLVAIERAPYDVQVHHLQL
                     KPKYDNPANLDVTLKIVTYAKV"
     gene            6032..6973
                     /gene="gspK"
                     /locus_tag="VLL06_03600"
     CDS             6032..6973
                     /gene="gspK"
                     /locus_tag="VLL06_03600"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HSS30082.1"
                     /translation="MRRSDERGIALLLTLLVLTLLVALILEFDAEARREYRDAAAFRD
                     NFKATVLARAAVQAARGVLQQDSVKEKQTGQAFDAPTDLWAFPITNYALGDGLLNAQI
                     EDELGKLNLNDLGIAGDLVAKAATVKKFKRLFELLQINPDLVDAIIDWVDDNEVQEPA
                     GAESLYYQAQRPSYKAANSKLQTLLELRLIKGITPEIITKLSKVVTVFPIDGGKPVNI
                     NTAGPLVLQALDQRITQSMATEIIQARPFKTKVDLDRVGSFQEIGMQIRTQYDVKSDF
                     FSARMTVTVNEVTRNATVVLQRDASTGISTVQYYRVL"
     gene            7375..7944
                     /locus_tag="VLL06_03605"
     CDS             7375..7944
                     /locus_tag="VLL06_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30083.1"
                     /translation="MKLTHLITFTASVCAVLSGCAANTPFPPEVMEKVVPTFQFEAWR
                     DAGPTNESGKSDSGIKVLLGGRIVQATKDSKGMVIVAEQLPIVNNPAYGPTDVNGTRT
                     GDHEFAFLYAGGLESLDLMKGNRFIIVGTTTSRRPILVNGIPKTEPFLVADCIHVWQT
                     GGLEISEFKEDAGGGYSPLPEKTYCSAKK"
     gene            7992..>9161
                     /locus_tag="VLL06_03610"
     CDS             7992..>9161
                     /locus_tag="VLL06_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30084.1"
                     /translation="MIRLGFATIACVISLGVIVQAETAQAGLEDLLYEKGQITKEEWL
                     KLQADREKAGPGAQGAPMPSSPALGPDPVSNLDQRLGMVEEWKAKIERLPSLGGKFNI
                     GLNALQFLYTHQNADTPEGKSQDNLSVRRSEVLMYGKVNEYIPKWHTLMEMQSINLTN
                     NTPDCTASPGGNCSGKAAGTGASTTMFREAYIDFRPMPSIAPNLNIIRMGVFRMPFGI
                     FTEQSGGLRDVISSPYLNSVGSGTVTRDGTGNSTVGTIDFIQERDYFADVRGTLFHKL
                     DYVFGIMNNNNFQANAVGANGPKVFYSRARFLATDISFVSFTVLTGESNNTNTEINGR
                     GKGAFDRYGIDFRYTSRYLPGLMVQGEWWQGHDGANQTTVGTPAQGACLNVVQCGG"
BASE COUNT         2143 a         2408 c         2578 g         2032 t
ORIGIN      
        1 atgctattct ctatcccgcc ttgatgatga tcgtcgggat ctcggtattg tttttcctca
       61 tgacctttgt cgtgccgaaa atcaccgccg tctttacgag tttgaaacag gctcttccct
      121 ggcctacggt cgtgctgatg ggtatcagca attttcttgc agactattgg gcggtgattc
      181 tcggtggagt ggtgttgatg gtgtgggcag tgcgtcgggc tatgaagaca gaaacggggc
      241 agctcacagc cgaccgatgg ctgctcaaga ttccactaat tggcgaggtc tcccgcatgg
      301 tcgcgatttc gcgcctcacg agtacccttg cgaccatgtt ggcgagcggt gtccagctgt
      361 tggacgcgat ggatgtcgcc aagcgtgtca tgaataatcg tgtgctggaa catgcagtcg
      421 agggggccag gcagaatatt cgcgaagggg aaacgattgc ggaaccgttg aaacgtagcg
      481 gagaattccc cgcgctggtc actcatatga ttgcagtcgg tgagcggagc ggtgaaatgg
      541 aagagatgtt gcgtcgaatc gggcacatct atgatggaga ggtggatcgc gtcattacgc
      601 ggttcacgtc gctgctggaa ccgattatga ttctcgtcat gggtgtcttg gtgttcttta
      661 ttgtggtcgc catcctgttg cccatctttg agatggggca gatggtgcgc tagacgggct
      721 ttgcccaagg aggcaggatg gggcaggatc gacatacaca tgagcggacc gttgagctgg
      781 aacttcggtc tgaccgaggg tttaccttta tcgaaatcat ggtggtggtg gcaatcctcg
      841 cgatcctcgc ggccttggtc atccctcgca tcatggggcg aaccgatgat gcgaagcgta
      901 cggcggcaaa ggttcagatt cgtaatatcg aaggcgcctt acagctctac aagctggaca
      961 acggtgtcta tccgtcaacc gaacagggat taaaagctct ggtcgaaaaa cctacagtcg
     1021 gagtaatccc gaagaagtgg aagattggcg gatatcttcc taaacttccc gaagatcctt
     1081 ggcaaaatcc ctataagtat ctgagcccca gtcagaagcg taccccgtcg ggaacccctc
     1141 tcggcgacta cgagctcatg tccctggcga ccgatgggga agtagggggc gaagggatca
     1201 atgccgatat cagcaatttt aatctcgaca aggactaagg catccatggt catcggttat
     1261 tcgtcagtgg ctggacatgc ccagacaggc tctgcacaca gggcgtttcc cgcattgcac
     1321 tcctcgcgtg gattcaccat tctggagatc atccttgtgc tcttcctcct cgttgtgctg
     1381 ctgggaatca tcctgcctcg aatttcactg gaggaaaatc tgggctccgt tggacggcag
     1441 atggtcggaa ccattcgatc ccttcaaagc ctagccatgt cgacccaaaa gacagtccac
     1501 ctatatgtgg atattgaaag aggggtctac tggccgatga tcatagatgg aagtcaggaa
     1561 aagcttccca ccgaccctac ctgggccatg cccttaaaac ttccggacac cattagaatt
     1621 tcagatatgt gggtggatca ggggaaaaaa gaatcagggc gtgtcgatct gttcttctac
     1681 cccacaggaa ggatcgatcc agcgacgatg catctggtga atacgaacaa tgccattcta
     1741 gccattgcca ttgagccggt cacgggagcg atccgcatga gcgatgaacg aattgagcct
     1801 ccaaagccgt tcatcatccc cgatcgtgtg aggccattac tcaagatcgc agtagcaggc
     1861 ccgtagtcgc gtcgatgcac agcacgcggt ccaccctaga gtgtgacgct atggggaatg
     1921 aacggggttt tacattattg gaggtcgtga tcgcactcgg catcctcgcc ctggctctgc
     1981 cgatcctcct gggacttcgc aattgggatc tcgacctcca tgcgagagcc aaggaaatga
     2041 ccgctgcgac gattctcgcg caggaaaagc tgatcgagac ggaattgggg acactcttgc
     2101 ctcttggtga aacgagtgga gaattcctgc cgaccccgct tggatcgcaa cctaccaccg
     2161 tggtcacgaa tcgccagtcg aattatcggt ggaagcgaat tgtctcttcc acgccattgc
     2221 ctatcgtgcg ggaagtgaag attcaagtcc tgtggccgcg gggtaccata gatgaaatgc
     2281 tcgaggtcag tacctatgtc ttttcgacaa cccccacgcc ttgaaggcgg ctttaccctc
     2341 gtcgaagtct tgctggccgt gtctctggtg gccatgatgg cggcgctggt atttgggtcc
     2401 ctctttgtca caacgagtgc gatcgatgcg gcgagagcga gctcggccaa tgagcagatc
     2461 attcgtagca ctatgcgtgt catgatcgat gaattgtcag tcggcaagag ctcccaggcc
     2521 ggcccttgga tggggatcaa cggccagttc gatggtcagc cggctgatac gattgccttc
     2581 ctcaccatgg gccagtttcg aggagcggag tcgaccaagg atacagaggt cgtgcgtatt
     2641 gtctacacga gagagggcga tcgcctcctt cgatacgtgc gaaagaatct ctacggtctg
     2701 acagatgaat ctgtcgggca aatcgaattg gccacgaagg tcaaagcgtt caacgtccgc
     2761 tattatgatg agaaaaaccg cctctgggtg gatgagtggg atggtcgtgg gcggctccag
     2821 tctgctccaa aggcaatctt gatcgagctg acgctgctcc aagagaacgc ggaactccaa
     2881 acgattcgcc agtgggtcac agtgggggca tcatgatcaa gtggtcgtgg cttgagacac
     2941 tgccgctgaa aggaccgttg gcttggattc tggcctcgct ggcttgcttt atcatatttc
     3001 tctttctgac ctttccctat ggcccgcttc aaaaccgcct gttgacggaa gtcaatcgag
     3061 cgaccggatg ggatgttcgt gccacggact ggtcggtggg ctttcctgcc ggcattgaat
     3121 ggcatgatgt cgtcctcgcc ggaccgccaa tgggggccat ttccatcaag gatgtgcgcg
     3181 caacaattgg agtctttgag gcaatcttgg gtcaactggt gctagactac gccgtccagc
     3241 ttccgggagg gacgcaagga ggtgccgggc gagccacggg atcgttgacg accgcctcat
     3301 ggcagttacg ggggcctgtt gccgtgaagg gtcacctcca gcagatggat cttgcaacgg
     3361 ttctgaaacc ctatgtgacc agcggaatga ttcagggaga tttcacccat cgatgggacg
     3421 gtgcgcaagg tagcaatgct gccctcaaag gggaagggac cgtgaaggtt gaaatcaagg
     3481 acttcgtgat cgaccagatt ccttccggca cccaatcaat gccctcactg acattcggcc
     3541 gcatccaagc cgcattcacc tgtcgcgatg cagcctgtga tgtgacggaa ttgaaaggag
     3601 acggtgttga tggatcgttt agcgtgcagg ggcatgtgac gttgcagcac ccgctacagc
     3661 aaagccttct ggaccttacc gtcaccgtcg ttccgggcgc tgggtttgcc caaaaagcgg
     3721 cgagcttctc gttgccgcca ttccagcccg ggacccaact cacgttcaag ttggtcgggc
     3781 cgatcatgaa tgctagagtg gcgctctgac agcatgttga taagttcagt ggcatgtgga
     3841 cacagtggtg cgagtgcgag tgcgtgagcc atgagacata gaagaataat ctcgtgatga
     3901 ggatgaggag cgcaagccgt tgtcgatgat tgctgaatgt gtcgggctcg atatcggaca
     3961 gacggccatt aaggcggtac ggtttcgtcg ccggctgacc gggcgtgaat cggtggagta
     4021 tttccaccta cccgtaccct atggacgccc ggaacagacg gagccggcct atcgagcggc
     4081 tatgctccga aattttctct ggcagcatgg gttgtatggg agcggagaaa ttgttaccgc
     4141 actcccctgt caagatgtgt ttgtcagaac cctctccttc ccctttcgtg atgccgccaa
     4201 gctcgcccag gttgtcccat tcgaaatgga aaatctgatt cccatgccct tggaggacgt
     4261 caccgtcggc agtctgttgt tgccggcgag agaaactcaa gaaggggact cgaaggctaa
     4321 aggttccgaa gttctcgtca cggccgcccc caaggataaa attgctgaac atctgcagtt
     4381 tatggcgtcc gctgatttga atccttctgc aatcacgatt gacggcatgg ccctattttc
     4441 tgtcagccag ttcctgaaag aagaaggggc ccaggttccc ggcgatatgg caattatcga
     4501 tgtcggcgcg tccaagacga ccctctgtct catacatgaa ggccgtcccg tattgttacg
     4561 gaccgtgttg tggggaggca atcatctcac tcacgcgctc gctgtccgct atgcctgtag
     4621 cttcgctgaa gcggaacgac ggaagcgggc catggcggta gaggaagtcg atacctggct
     4681 cgaacctgtt ctcaaagaat tacgcgtctc cctccattcc tatgaaggga ccgtacatca
     4741 acggttatct cattgctggg tctcgggagg gggctcgaaa ctgaaagaac tcagcggaca
     4801 tatggcgcgt caactgggat tgctgccggt cggcccgcgg caaggatttg gagccggctg
     4861 tccccgcgcc ttttccatcg catttggatt ggccatccat ccgaatatcg tcagacctcg
     4921 ctggaagtcg aggctggccg ggtcaagtct tgctctggat ttcaaggcca gcagcgatgc
     4981 tgcatcggca gcgggacatg tctcgaagca ggatcgtcgc ttggctctgt ggggcgcact
     5041 tattctcggt attctggcgc tgagcgatct ctccgttcgc gtcatgctga aggattcgac
     5101 ggtcaagaac ctcaagggag agatccaggc tcaatatgag caaacgttcg gtcccggcgc
     5161 gaacgctggt gaagaattgg accaggcccg gtatcgagtc tcacaggtcg agaaaggact
     5221 ctcggtggtt gatggcacac gctacaatgt ccttgcgagc cttgcggaac tggtgaagca
     5281 tgtgccgcca ggcacaccat tgacgattcg tgaactgacc atagacggga ccagcatcca
     5341 tctggagggg gaaaccacga cgttcgatac ggtcgagaag atcaagcagg cctttgtggc
     5401 gacggattca tttcaagacg tcaccattag tgacacgcgg gtcggagccg tgcagaacca
     5461 agtcgtattt cgcctgacct atacggtgca acggccatga tgcagatgct aaaagaacgg
     5521 tggaatcatt tctcacaacg cgagcgcatc atcgtggcca ccggcatgac tgctcttgcc
     5581 gccgcgctcg tcttcttgct gatcatcgat ccactcatgg acacaatcga caaactcgat
     5641 cgacaagcgc gccgaaaagc aaaagacagc caggagctta tgatcgtggc gcaagagtat
     5701 gtggccaagc aggcccgcat tgccaaactc gaagagcgca tgccgaaccc gccggctcag
     5761 ttttctctgc tggccttcat ggaagaagcg accacgacgg cacaaattcg cgatcggatc
     5821 ggcggcatgc agccacatgc tcctatggtc gtgcagggtt atcaggaaac cgctgtcgat
     5881 cttcggctcg acggtgtctc gcttccgcaa atccttgcgc tactcgtggc catcgagcgg
     5941 gcgccttacg acgtgcaagt gcaccatctg caactgaaac caaaatatga caatccggcc
     6001 aatctcgacg ttacgttgaa aatcgtgacc tatgcgaagg tctgatgaac gcggtattgc
     6061 actgctgctt accctcctgg tcctaactct cctggtggca ttgattctcg agttcgatgc
     6121 cgaagcgagg cgggaatacc gcgatgccgc agcgttccgc gataatttta aagccaccgt
     6181 gctcgcgcgc gcggcagtcc aggcagcccg tggcgtctta caacaggaca gcgtaaagga
     6241 gaaacaaacg ggacaggcct ttgatgcccc aaccgaccta tgggcatttc ccatcacgaa
     6301 ttacgccctc ggtgatggcc tgctcaacgc gcaaatcgaa gacgaactgg gcaagctgaa
     6361 tttgaacgac ttgggtatcg ctggggatct tgttgccaaa gcggccacgg tgaaaaaatt
     6421 caagcggctc ttcgagctct tgcagatcaa tcccgattta gtcgatgcga ttatcgattg
     6481 ggtggatgac aatgaggttc aggaacccgc cggcgcagag agcctgtact atcaggcgca
     6541 gcggccctcc tacaaggcag ccaatagtaa gttacagaca ctcttagaat tgcgtctgat
     6601 caaggggatc actcccgaga tcatcacgaa gctctcgaag gtcgtgaccg tgttcccaat
     6661 agatggtgga aaaccggtga atatcaatac ggccggccct ctcgtgcttc aggccctcga
     6721 tcagcgtatt acacaaagta tggccacaga aattatccag gctcgtccct tcaagaccaa
     6781 agtggattta gatcgtgtgg gcagttttca ggagataggg atgcagatcc gaacccagta
     6841 tgacgtcaaa agcgactttt tctcagcccg catgaccgtg actgtgaacg aagtgaccag
     6901 aaatgcgacc gtcgtgctgc agcgcgatgc aagcactgga ataagcacgg tgcagtacta
     6961 tcgcgtcctc tagcgcctct ctgctccccc gcgtgtgaaa ccgaagaaat cgacgcatgc
     7021 agcagaaccc gcgcacacgt cggccaatct tgatctacgt ttttgattaa ccgatcaatc
     7081 agacgagatc acgagagtac cagccgaaga agatctgcct cgcaacgaga taaacacggg
     7141 atgctcgacg ccttcctcaa aacgtaatac ccctcccaag caaaacgcgt cgcaagaact
     7201 ttatgcagcg cgcaatttat taacgcgctg ttaataattt aacgcgtcgt taacactccg
     7261 gtatcaatct cataacatgc aggaaatata tttccatgag gtcctgatcc gcgaatcaca
     7321 tccatggcga gttcgaactg agtcaacaat tgtcttcaaa ctggagctag cactatgaaa
     7381 cttactcatc tcatcacatt cacggcttca gtatgcgcag tgctgtctgg atgcgctgcg
     7441 aatacaccct ttccaccaga agtcatggag aaggttgttc cgacctttca gtttgaagcc
     7501 tggcgtgacg caggtcctac gaatgagtcg gggaaatccg actcagggat caaagtcctg
     7561 ctgggaggcc ggatcgtcca ggctaccaag gacagcaagg gcatggtgat tgtcgcggag
     7621 cagcttccaa tcgtgaacaa tcccgcgtat gggcccacgg atgtaaatgg cacgagaaca
     7681 ggtgaccatg aattcgcatt tctctatgcg ggaggacttg aatctctgga tctgatgaag
     7741 ggaaaccggt tcatcattgt gggaacaacc acgagtagac ggccgattct ggtgaacggc
     7801 atacccaaga ccgaaccctt cttagtcgct gactgcatcc atgtgtggca aacaggcggg
     7861 cttgaaatct cagaatttaa agaagatgcc ggaggaggtt attcgcccct tccggaaaaa
     7921 acatactgct ccgcgaagaa gtaactggat cagaaagaag ctattcgaaa tgtcacgatg
     7981 gaggtatttg aatgatacga ctgggattcg caaccattgc ctgcgtgatc agtcttgggg
     8041 tgattgtcca ggccgaaact gctcaagccg gccttgagga ccttctctat gagaaggggc
     8101 agattaccaa agaagaatgg ctcaaacttc aagccgatcg tgaaaaagca ggaccgggag
     8161 cacaaggggc tccgatgcct agttcccctg cgctcggacc agatcccgtc tcgaatctcg
     8221 atcagcgctt aggcatggtt gaagagtgga aagccaagat cgaaagactt ccctcgctcg
     8281 gcggcaagtt taacatcgga ttgaatgccc tgcagtttct ctacacacat cagaatgccg
     8341 atacccccga gggcaaaagc caagataacc tgtcggtccg acggtctgag gtactcatgt
     8401 atgggaaagt caatgaatac attcccaaat ggcacaccct gatggaaatg cagagcatca
     8461 atttgaccaa caacacaccg gattgcactg cctcgccggg aggcaactgc tccgggaagg
     8521 ccgcagggac aggtgcttcg accacgatgt tccgagaagc ctatatcgat ttcaggccga
     8581 tgcccagcat cgcgcccaat ctcaacatca ttcggatggg tgtgtttcgt atgcccttcg
     8641 gcattttcac ggaacaatcc ggtggtctcc gcgatgtcat cagctcaccc tacctcaact
     8701 cggtcggcag tggcacagtg acgcgagacg ggactggaaa tagcaccgtt ggtacgatcg
     8761 acttcattca agagcgcgac tattttgccg atgtcagggg caccctattc cacaagctgg
     8821 actatgtgtt cggaatcatg aataacaata acttccaggc caacgctgtg ggagcgaatg
     8881 gccccaaggt cttctactca cgagcccgtt ttctggcgac ggacatttcg tttgtcagtt
     8941 ttacggtact gacgggagag agcaacaaca cgaataccga aattaacggg cgtgggaaag
     9001 gagcctttga ccggtacgga attgatttcc gctatacctc gcgctacctt cctggattga
     9061 tggtgcaggg cgaatggtgg caggggcatg acggagccaa tcaaaccacg gtcggcaccc
     9121 cggctcaagg agcgtgtttg aacgtggtcc agtgcggtgg a
//
LOCUS       DATIPF010000138         4400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_26624, whole genome shotgun sequence.
ACCESSION   DATIPF010000138 DATIPF010000000
VERSION     DATIPF010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26624"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1027..1608
                     /locus_tag="VLL06_03615"
     CDS             1027..1608
                     /locus_tag="VLL06_03615"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS30085.1"
                     /translation="MKYFSILAFLACVPFAGCALFSPPNTISAEDLQTLQTQAAQGNA
                     KAQIDLGQRYHFGQGVPQDYEKAREWYEKAATQGDATGQNSLGTLYQAGKGVPQDDAK
                     ARDLYERAAAQGNALAMYNLGWLYGKGKGVPPDYVKSYMWSDLAAAGAPSRGALAQAV
                     RSREMIAIRMTPAQIAEAQRLAQQCEAQQFKGC"
     gene            1906..2868
                     /locus_tag="VLL06_03620"
     CDS             1906..2868
                     /locus_tag="VLL06_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30086.1"
                     /translation="MAAAHQTAPEQISVKLTGFADSEQANRFGHAIAETVRLISTVID
                     LERLDGITVSFDYDAALASLDRGFNETAPLKKTSTDLLLGVAMSAPVLREGVVKAHMI
                     YYAPVVTALDGGDEESQRRALSLLAHECGHVEAIKHSDMAFPDRMFRPVHGKIHFLNE
                     IAESIWSEYSACRISAIFGKELTSIYEDSFVSVLRVARDRTNAAIREYRLHADLSRVL
                     QEAGQHICQPLKIGAYLFGHLDGLKLEFDVVPTTEAALRETQYDRCMNEMHEALRELW
                     SRLGRWSDVAEFDVLENIAREALAERGMIFTAIDDERWHLDIPP"
BASE COUNT         1004 a         1176 c         1232 g          988 t
ORIGIN      
        1 ggagtcaaga tggaaaacac ggcggtctga gccttggcga gaggcgagga ggcttgccaa
       61 gcttcggtta gaacctcaca agcgacgacg ctcaccaaaa tggcgcatac ggtcgaacga
      121 tctccgtgcc acatgcctcg cagaaataac gcccccatcg tcgggccagt gtcgtcgggt
      181 gacaagtcga cgaggcgttg aacggtgtgc gtgcaatcta tcgccatatc tgatggacgc
      241 gcccatttgt gcagacagga aaccttctga caggcaagga tgggctgcgg cggtgattgg
      301 acgctaccat tggcggacgc catccgccag atcgtgactg gttttacatt gtcgctattg
      361 cactgggggc agatcgtcga atgaggcatt tgccttccct ggtagttcaa gggttggagc
      421 aggcatttgc cttcgcgtgc agtgttgatg tttgtcatat tatatcggga attagtgccg
      481 acataccctg ggaaccttcc tagtccgcca tgccggcagg cgggtcccag cgcattcggc
      541 aaggacctgc ctgacgatcc agacccatgg ccatgctaag tatggccgca cgctggccga
      601 tgtgccctgc cccgatggga ggaacctcaa tcaggaactc gtcaagcaag gctggtgctg
      661 gtggtagcgg aaatatgcac cgagggatac ggtgcttgag gggctagaga ggaacgcacg
      721 agaggctaag agaggcttgt gggctgatcc gcagccggtg ccgccgtggg agtggcggaa
      781 gcgcagagac taagcgacca aggattacag atgcagacgt tgacaccttc ccacggtggg
      841 cctagccttg cggtcaatca accccgttgc ggaagtgttg gcgtcaccga gaccgaaaca
      901 atccatgatt cctgttcaca ccatcgtctt catgctctca ttcgtctatc tcgccgtttc
      961 cgcactggcc tccgcgtggc aagaagtagc ggagtttctc tcacttctca ggatgggaga
     1021 ctcattatga agtacttctc aattctggcc ttccttgctt gcgtgccatt tgcaggctgt
     1081 gcactgttct ccccaccgaa caccatttca gcagaagacc tgcaaacgct ccagacacag
     1141 gcggctcagg gcaatgcaaa ggcgcagatc gatctcgggc agaggtacca cttcggacag
     1201 ggcgtgccgc aggattatga aaaggcgcgg gagtggtatg agaaagcagc tacccagggc
     1261 gatgcgacgg gacagaacag cctcggaacg ctgtatcaag caggaaaagg tgtgccgcag
     1321 gacgatgcca aggcgcggga cttatatgag cgagccgccg cacagggtaa cgcactggcg
     1381 atgtacaacc tcggttggtt gtatggcaag gggaagggtg taccgccgga ttatgtgaag
     1441 tcgtatatgt ggtcagacct cgcggcagca ggcgccccgt cacgtggggc gctggcgcag
     1501 gcagtacgca gccgagaaat gatcgccatc cgcatgactc ccgcacagat cgcagaggcc
     1561 cagcgactcg cccagcagtg tgaggcacag cagttcaaag gttgctgata cgggtctgcc
     1621 ggaacaaaag atggatgtat caaaaagaag tgagggggga ctcggaaggt ccactcaatc
     1681 acatgccgcc gtgggagtgg cgcagggcgt ggcgggtgca gcggtagcgt cacgaggccg
     1741 gtttcatacg gtctccgcct cgtcgatcgc gcgattagat tagggaaggc cggtaggccc
     1801 tctatggtca ggatttggct gtcccgctgc acgagcatcg tgatgcacgt atcattcaac
     1861 tcaatctcct agcgtggtcg tccgtggcca cgatcgagga gcgcggtggc agcggcacac
     1921 cagactgctc cggaacaaat ctccgtaaag cttacgggat tcgccgattc cgagcaggcc
     1981 aaccgatttg ggcacgcaat cgctgaaacg gttcgcctca tcagcacagt aattgattta
     2041 gagcgcctgg atggcattac ggtgtcattt gattatgatg ccgcattggc atcactcgat
     2101 cgtggattta atgagacagc tcccttaaaa aagacgtcca ctgacttgct tctcggtgtc
     2161 gccatgtctg ctccagtcct gcgagaggga gtagttaaag cgcatatgat ctattacgct
     2221 cctgttgtta cggcattgga tggcggtgat gaggagtcac agcgccgggc gctttctttg
     2281 cttgcacatg agtgtgggca tgtcgaggcg ataaagcatt cggatatggc tttccccgac
     2341 agaatgtttc gcccagttca cggtaagatt cactttctaa acgagattgc cgagagcata
     2401 tggtctgagt actctgcttg ccgaataagc gcgattttcg gaaaagagct gacgtcaatc
     2461 tatgaggatt cttttgtatc tgtcctgagg gtggctcggg atcgaacaaa tgcggccata
     2521 cgggaatacc gacttcacgc tgatctaagc agagtactgc aagaggccgg ccaacacatc
     2581 tgtcagcccc taaaaatcgg cgcctatctg tttggacact tggacggtct caaactagaa
     2641 tttgacgttg tgccaaccac tgaggctgcc ttacgggaaa ctcaatacga tcgatgtatg
     2701 aatgaaatgc atgaggcgct acgcgaactg tggtctcgcc ttggtcgttg gtcagacgtg
     2761 gccgagtttg acgtgcttga gaacattgcc cgcgaagctc tggccgagag aggaatgatc
     2821 tttactgcta tcgacgacga aaggtggcat ttagatattc ccccttaatc ctgaaacgat
     2881 tcctcactta tagccaggcg aagagggaac cccattgccc cgacgttctg cctgcaagta
     2941 tgcactgatg gttttgtgtc tccaatcgtg cggtgagtgg ggaaactctg aacagtaaca
     3001 gtggtgccat ctcactgagt ggactccatc gcagatggga cccattcaga aacgaaggcc
     3061 gggggtctgc tcgctctgcg tctgtgggtc ccatctactc gggggtttcc ttttgtcgca
     3121 ggaatggaaa tcgagaagag gccccttgtt tcctggcggg agaaaggtgt gtgtctatgg
     3181 ctagtcgtgg aggctcatga aggccacccc gcctgccgat tgtcatgtaa ttgtcatgtg
     3241 gctgccctat cccgctgtgc tctgctgtgt cacgctgttt cagcgatccc tgtaaacaca
     3301 atggattatc taacggtaca ccacagggag acacggcacg ccacacactg cgctcccttc
     3361 agggtctagc gcccgcaagg gtgtgcgggt tcaaaatccc gccttcggca ccaactcaca
     3421 ttccccattc cccaaggagc gtcagcattt tcacaccttc atattgaaca ccggagaaca
     3481 cctcttcggg ggtctgttta acttcgattg tcatgtagct agggaacttt ctagtctcga
     3541 tgtctgtaag tgcgtagtac tatccgtcgc atgactaaag ctcaggccgc tctccttcaa
     3601 acgaagtgga agcaacaagg cgatcctccg ccagcctgtc aacattcgat ccaagaattg
     3661 gcgtcgtctg agttggataa ccatgtccat gtgttgaaca cctatcattg tcgcgattgt
     3721 ggagagctaa ttgttcacag gctcaaggga aagggcttgt ccaacagccc tttgatggaa
     3781 cctgagtgct tcaacgagct ataaagaagt ctaagtcatc gaaacacccg tcacattgtc
     3841 gcccccttta gcacctgcca ttagttcctc ctttttgaac tcgggacgtt actagttccc
     3901 attctgttcc tatcgcgaat agcatgcact catgatctgt ccaaaatgtc ggagtcatca
     3961 taaggccgaa ccaaccgagg gttggatgat ctccggccgt gaattgattc ccctcttgca
     4021 cctgtggacc tgtgggaatc tggcatgtcg gcaccagtgg ccgaggctct ctctcgtgcc
     4081 acgatccaca catccgaaac catgtccaag gtttacttcc ccacctggac cgacccaggc
     4141 tgcatagagg gccatccgtc cagcattcct gtaagcgcct ccacagccaa cgacgcgttc
     4201 cctaccgcca gattattgcc aggacgtacc cgcgcgcacc gtctctgtcg actgtcggcc
     4261 acactaacta attgcacgac ggtcatataa accacgacat agttagtgta atcaaactaa
     4321 aagatatcct agctgtgtcg ataatgactt cggccctcac tcggctacaa gaatcatagc
     4381 taacaagaga gtgagtgttg
//
LOCUS       DATIPF010000139         4718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1704599, whole genome shotgun sequence.
ACCESSION   DATIPF010000139 DATIPF010000000
VERSION     DATIPF010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704599"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /gene="carB"
                     /locus_tag="VLL06_03625"
     CDS             complement(<1..449)
                     /gene="carB"
                     /locus_tag="VLL06_03625"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249075.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HSS30087.1"
                     /translation="MPRRTDIQSILLIGSGPIVIGQACEFDYSGTQACKALKEEGYRV
                     VLINSNPATIMTDPELADRTYVEPITIEVVEKVIERERPDALLPTMGGQTALNTTMGL
                     VKRGTLEKYEVKLIGASAEAIYKAEDREAFKHAMQRIGLRVPASGTA"
     gene            complement(472..1437)
                     /locus_tag="VLL06_03630"
     CDS             complement(472..1437)
                     /locus_tag="VLL06_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HSS30088.1"
                     /translation="MMQSSDDNIDRFFATPIGRRTLLALGTHGATSLCTAFGIGAALS
                     LFGSLTGCYRAPGTARDQLIFFSEENELQFGLSAFRDVLRSAPLSDNAEINDLVHRVG
                     RNIAEAANKPEYQWEFAVIQDDKMVNAFALPGGKVAIFTGILKHTKDDAGLATVMGHE
                     VAHALQRHGVERMSRSILDQIAQLGAMGAAATGKANAGAIQGLLGAYGVNVSLPFNRK
                     QESEADYVGLRLMAQAGYDPREAVPFWERMSGCPRQMIGKVCFRSQQAVPEFLSTHPS
                     DLTRINQIEAWLPEALQYYHGPGGGMAPPPPPPYKPTIGPMPQSS"
     gene            complement(1490..2458)
                     /gene="sppA"
                     /locus_tag="VLL06_03635"
     CDS             complement(1490..2458)
                     /gene="sppA"
                     /locus_tag="VLL06_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HSS30089.1"
                     /translation="MFRPYAMLGRFCLLAAIGLQAGCLTINLPGPLGPVKETELSGKG
                     EGKVLLLELSGVISSQDKEGIIPQPNMLATFKEELTRAAKDDKIKAVVVRINSPGGTV
                     TASDILYHELREFKTKKKVPVIASMMDVAASGGYYLAMAADSILVHPSTVTGSIGVIM
                     LTVNARGLLEKVGVEASAITSGPRKDMGSPFRVMTAEERGIFQGVIDSFYQRFLAVVQ
                     EGRPNLSADQIKKLADGRIYSGDQAKAAGLIDEIGYLDDAIEMAKKKAGLTEARVVTY
                     GRPGEYQNNIYSRVFGASPSIAGLVNFDLLSMVRGGTPQFMYLWMP"
     gene            complement(2451..3626)
                     /gene="carA"
                     /locus_tag="VLL06_03640"
     CDS             complement(2451..3626)
                     /gene="carA"
                     /locus_tag="VLL06_03640"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HSS30090.1"
                     /translation="MKKALLALADGTIFEGRALGYEGETVGEVVFNTAMTGYQEVLTD
                     PSYKGQIITMTSPHIGNYGMTQEDAESRRVWAEGFIVKEGSQLASNWRSRQQLQDYLS
                     EAKIVAIEGLDTRALTRHLRDQGSQQGLITHVGGDSRHAIEKAQAAPSIIGRDLAAVV
                     TCEKAYRWTEGSREWAPSLGDKMEVGATSRWHVVAYDFGVKRNILRRLVDVGCEVTVV
                     PASTTAAQVEALKPDGIFLSNGPGDPEGVPYAVEAMRQLIGRYPIFGICLGHQILGLA
                     LGLKTYKLKFGHHGANHPVMDLRTRQVEITSQNHNFAVQVMPPINAIPDRPPVVNTAF
                     GQVAITHLSLNDHSIEGLVCLDRPVFSVQYHPEASPGPHDSAYLFTEFIQLMEKQHV"
     gene            complement(3623..4135)
                     /locus_tag="VLL06_03645"
     CDS             complement(3623..4135)
                     /locus_tag="VLL06_03645"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HSS30091.1"
                     /translation="MSRWGMVCCLMGEWLALAVWVGGLLVLIGAVIPAVFNTFGGQDS
                     GGLFLTRAFEGYNHFVIGAIAVLSAGLWYRWWCGDPAVAVSRSELAVLAGMVVIAGII
                     IVVLHPYAAALQVQAFAVKEEAARKAAFEALFRILMPGRSLYIVNLVLGILLIGIKAK
                     RSLDRDKVLT"
     gene            complement(4149..>4718)
                     /locus_tag="VLL06_03650"
     CDS             complement(4149..>4718)
                     /locus_tag="VLL06_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSS30092.1"
                     /translation="RMVREAKSRGLKVTAEACPHHFTITEEVVRGYNTYAKMNPPLRT
                     WKDVQAIKEGLRDGTIDVIATDHAPHATQEKQLGFTEAPFGIVGLETALSLTVALVEE
                     GVLSLESAVDKLSTAPAKVFGLAKGTLAVGADADVAIVDQQEQWEVDPAKFRSKSRNT
                     PFAGWKVKGRVRTTIVGGRVVFEAGPAEP"
BASE COUNT         1006 a         1525 c         1172 g         1015 t
ORIGIN      
        1 tgtgccgtgc cgctggccgg cactcgcaat ccaatccgct gcatggcgtg tttgaaggct
       61 tcgcgatcct cggccttata aatcgcctcc gcagacgcac cgatcagctt gacctcatat
      121 ttttcgagcg tcccccgctt gaccaatccc atcgtcgtat tcaacgcggt ctgtcctccc
      181 atggtcggaa gcagagcatc gggccgttcg cgttcgatca ctttctcgac cacctcaatc
      241 gtgatcggct ccacatacgt ccgatccgcc aattcgggat cggtcatgat cgtcgccggg
      301 ttgctgttga tgagaaccac ccggtagcct tcttctttga gggctttaca ggcctgcgtg
      361 ccggaatagt cgaactcgca ggcctggccg atcacgatcg gccctgaccc gatcagcaag
      421 atggactgaa tatctgttcg tctcggcacg aagacctcgt ctaagagtca gctaactcga
      481 ttgtggcatt ggcccgatcg tcggcttgta tggcggaggt ggcggcggag ccatccctcc
      541 tccagggcca tgatagtact ggagcgcctc aggcagccag gcttcgatct gattgatacg
      601 cgtcaaatcg gacggatgag tggacaaaaa ctcgggtacc gcctgctgcg agcgaaagca
      661 gaccttgccg atcatctgcc tcggacagcc gctcatgcgc tcccaaaacg gcacggcttc
      721 acgcggatcg tacccggcct gcgccatcaa acgaagtcca acgtagtcgg cttccgactc
      781 ttgtttccgg ttgaagggca gcgacacgtt caccccatac gcccctaaca agccctggat
      841 tgcaccggca ttggcttttc cggttgccgc cgcacccatg gcacctaact gcgcaatttg
      901 atccaggata ctccggctca tccgctccac cccgtggcgc tgcaacgcat gcgcaacctc
      961 atgccccatc accgtcgcca agccggcatc gtctttggta tgtttgagaa tcccggtgaa
     1021 gatggcaacc ttcccgcctg gaagggcgaa ggcattgacc atcttatcgt cttgaatgac
     1081 cgcgaattcc cactgatact ccggtttgtt cgcggcttcc gcgatgtttc gtcccacgcg
     1141 gtgcaccaaa tcgttgattt ccgcattgtc gctcagcgga gccgaccgca acacgtctcg
     1201 gaatgcgctc aatccaaatt gcagttcgtt ctcttcggag aaaaagatca actgatcgcg
     1261 agccgtgcct ggagcccggt agcacccggt caggctaccg aacagactca aggctgcccc
     1321 aatcccaaag gccgtgcaga ggctcgtcgc cccatgggta cccaacgcca gcagggtccg
     1381 acgtccgatc ggcgtcgcaa agaacctgtc gatgttgtcg tccgatgatt gcatcattgc
     1441 ggatcacttc acggaactct tcgacccatc gctcaaggca ccatgttgat catggcatcc
     1501 agaggtacat gaattgcggt gttccacccc gtaccatcga cagcaaatca aaattgacta
     1561 acccggcgat gctcggactt gctccgaaca cacgcgaata gatattgttt tgatactccc
     1621 caggccgccc ataggtcacg acccgcgcct ccgtcagacc tgccttcttc ttggccatct
     1681 caatggcatc atcaagatac ccgatctcat cgattaagcc tgccgccttc gcctgatctc
     1741 cggagtagat ccgcccgtca gccagtttct tgatttgatc agccgacaga ttgggacgcc
     1801 cttcctgcac cacggccagg aaccgttgat agaacgagtc gattacacct tgaaagattc
     1861 cccgctcctc agctgtcatg acccgaaagg gggaacccat gtccttgcgg ggtcccgacg
     1921 tgatggcact ggcctcgaca cccacttttt caagcagccc acgggcattg acggtcagca
     1981 tgatcacacc gatactgccc gtcaccgtcg agggatgaac caaaatgctg tccgccgcca
     2041 tggcgagata gtagccccct gacgccgcca catccatcat tgacgcaatc accggcacct
     2101 tcttcttggt tttgaactcc cgtaattcat gatacaggat gtccgaagcg gtcacagttc
     2161 cccccggact gttgatgcga acgacgacgg ctttgatctt gtcgtccttt gcggccctcg
     2221 tcagctcttc cttgaacgtg gccagcatgt tgggctgcgg aatgatacct tctttatctt
     2281 gcgagctgat gactccagac agctcgagca acaagacttt tccttcgccc ttgccgctga
     2341 gttccgtttc cttcaccggc cctaatggac cgggaagatt gatcgtgagg caaccggctt
     2401 gcagcccgat ggctgcaagg aggcagaacc gaccaagcat tgcgtacggt ctaaacatgc
     2461 tgtttctcca ttaactgaat aaactcagtg aataagtagg cggagtcatg gggaccggga
     2521 gacgcctcgg ggtgatactg gaccgaaaac accggacggt ccagacagac caatccttca
     2581 atcgagtgat cgttcaaact cagatgtgtg atcgcgactt ggccgaatgc cgtattcact
     2641 acgggaggtc gatccggaat tgcgttgatc ggcggcatga cttgaaccgc gaaattgtgg
     2701 ttttgcgagg tgatctcaac ttgcctcgtg cggagatcca tgaccggatg attcgctcca
     2761 tggtggccga acttcagctt ataggttttg agtcccagcg cgagaccgag aatctggtgg
     2821 cctaaacaaa tgccaaagat cggatagcgt ccaatcaact gccgcatcgc ctcgaccgca
     2881 tagggaacgc cctcaggatc ccccggacca ttcgacagga aaatcccatc gggcttgagg
     2941 gcttcaacct gcgccgcagt ggtcgaagcc ggaacgacag tcacctcaca tcccacatcc
     3001 accaaccgac gaagaatatt ccgtttcact ccgaaatcgt aggcgacaac atgccaccga
     3061 ctggtcgccc cgacctccat cttgtcgccg agcgagggag cccactcgcg tgacccctct
     3121 gtccaccgat aggctttctc acaggtcacg acggccgcca gatcacgccc aatgatgctc
     3181 ggagctgcct gggccttttc gatcgcgtgg cgggaatccc caccgacatg ggtgatgagg
     3241 ccttgctgtg aaccttggtc acgcaagtgg cgggtcaggg cccgcgtatc cagcccctcg
     3301 atggcgacaa tcttcgcttc agacaaatag tcttgtaact gctgtcgact gcgccagtta
     3361 ctggcgagtt ggcttccttc cttcacgatg aatccttcag cccacacccg cctcgactcg
     3421 gcatcctcct gggtcatccc gtagttccca atgtgcggcg aggtcatggt gatgatttgt
     3481 cccttatacg agggatcggt caagacttcc tgatagccag tcatcgccgt gttaaacacg
     3541 acttccccca ccgtctcacc ttcgtatccc agggcacggc cctcgaagat ggtgccatct
     3601 gccaatgcca acaacgcttt tttcacgtga gcactttgtc cctgtccagt gaccgtttcg
     3661 ctttgattcc gatcaggaga attcccaaca caagattgac gatatacagc gaccggccgg
     3721 gcatcaggat acgaaacaag gcttcgaagg ccgccttcct cgccgcctcc tctttcaccg
     3781 caaaggcctg aacttgaagc gccgctgcat agggatgcag caccacaata atgatgccgg
     3841 cgatcaccac catccccgcc agcaccgcca actcgctccg actgaccgcc accgccggat
     3901 ctccacacca ccagcgatac cacaatcctg cactcaagac cgctatcgcc ccaatgacga
     3961 agtgattata gccttcaaat gcccgcgtca gaaacaatcc acccgagtct tgcccgccga
     4021 acgtattaaa caccgctgga atgaccgctc cgatcagtac caggagaccg ccgacccata
     4081 ctgccagcgc caaccattcg cccatgaggc aacagaccat cccccaacgg gacatacgct
     4141 gcaccgcgct atggctccgc cgggccggcc tcgaacacca cccgtccacc cacaatcgtc
     4201 gtcctgaccc gacctttcac tttccatccg gcaaacggag tatttcggct ctttgaacga
     4261 aacttggccg gatcgacttc ccactgttcc tgttgatcga cgatcgccac atcggcatcg
     4321 gccccgaccg ccaatgtgcc tttggccaat ccaaacactt ttgccggagc cgtactcaac
     4381 ttatcgaccg ccgactccaa tgacaagact ccctcttcca ccagcgcgac ggtcaatgac
     4441 agtgccgttt ccaatccaac gataccgaat ggcgcttcgg taaatcccag ctgtttttcc
     4501 tgagtggcat gcggggcatg atccgtggca atgacatcga tcgtcccatc gcgaagaccc
     4561 tccttgatcg cctgcacatc cttccatgtc cgaagcgggg gattcatttt ggcataggta
     4621 ttatagcctc ggaccacttc ttctgtgatg gtaaagtgat gggggcaggc ttcggccgtg
     4681 actttgagcc cgcgcgactt ggcttcgcgc accatccg
//
LOCUS       DATIPF010000140         4278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_468317, whole genome shotgun sequence.
ACCESSION   DATIPF010000140 DATIPF010000000
VERSION     DATIPF010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468317"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="VLL06_03655"
     CDS             <1..1184
                     /locus_tag="VLL06_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSS30093.1"
                     /translation="VKQTQPMTGGRIVVAVDGSPYSFGGLMTGLALGKAFNMPVEAIS
                     AFDPYFHYAAFHSISGVLNEEAGKVFRFKEQEKLHEEIIDSGLAKIYQSHLDICREIA
                     QAEQTDVKTTLLDGKAFEKIIQYVRKDIPALLIVGRIGVHSDEDMDIGSNTENLLRSA
                     PCNILVSNRKYVPPIDTLAEYTIAWTEEALRRMEKIPIFARGVAKTAIHRYAIEKGHT
                     IISNTVVDTAIGHILPKGAMDAMRALGGNLDAAGIDRNKMQADESVVKDLMGSTLSGM
                     MTEVVEEKPTVSANTQAYLDRMSQDYFICDGCGYIGKGERPVKCPVCAAGGTASSWLT
                     SLSSKQRRMLKGPSRQTWPTMMSPCNGRRMLKKESVLFQQAFNAGGPKPRSRRPPANL
                     A"
     gene            1076..1651
                     /locus_tag="VLL06_03660"
     CDS             1076..1651
                     /locus_tag="VLL06_03660"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HSS30094.1"
                     /translation="MQWTKDAKEGIRAVPAGFQRRRAKAKIEKTARKLGMTTITLEYA
                     APMIQEAASEDYTPIFANKGTGASMEAQATVAVAANGTNGATVAHENGHGSGNGHAAE
                     SAPAPASPYTWLPEAQSRLERAPEGFMRDCTRALIEKHADKLGVMVITAEVAHEGIEQ
                     AKDYMAEAMKTGNLKDMIANLTGSSKPKAGH"
     gene            1651..3036
                     /locus_tag="VLL06_03665"
     CDS             1651..3036
                     /locus_tag="VLL06_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSS30095.1"
                     /translation="MGRSLPVLNFPALAGTLGSIQQQVSQFFTPPTTAPAPHDGRTVD
                     DFKPYLIALNLTKRCNLKCDHCYLDATTKSAGGNDELSTEECYKLIDQIAEVNRGCLL
                     VITGGEPLVRPDILDIARHAVKLGFMVVFGTNGMLIDDRMAKDLVDIGVMGMGISIDS
                     LDPQKHNRFRGVPGAWEAALAGIEACKRNGLAFQVHFSAQPMNYRELPEVIDWAHQLG
                     AKVLNVFFMVCTGRGEELTDITPAQYEEVLGYLVNCQDNYKGMLVRARCAPHFKRLAY
                     EKDPNSPITKATGYMGGGCLAGTNYARVTPNGELTPCPYMPLSAGNIRETSFVDLWEK
                     SDVFNSFRYPQLKGKCGDCEYTDICGGCRARPYVDHGDWLDEDQWCLYTPKGGEKIKV
                     AFNTPEEGDVAWDEDSETRLNRIPYFLRAMVKKGVEKHARENGIPLITIELMEELRKK
                     RFGNDAPVFKF"
     gene            3108..3620
                     /locus_tag="VLL06_03670"
     CDS             3108..3620
                     /locus_tag="VLL06_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30096.1"
                     /translation="MDALQSLVTDPHTFIPIINHWLHLVSAVIWIGGLAFLVIAVTPG
                     LKAAVAKDQVKPITDAFYHHYKKVAGILLMVLLFTGGINLHYVNQIVTAQSGSGIQHH
                     ATYLIVFFIKLFLVICLLTLFLYTVFFKSDEAEEGDEESYEAIPFQRAALWIGFFIIL
                     CAAAMKHLHQ"
BASE COUNT         1019 a         1199 c         1136 g          924 t
ORIGIN      
        1 tcgtcaagca gacccagccc atgaccggcg ggagaatcgt ggtggcggtg gatggcagcc
       61 cctattcctt cggcggactc atgaccggct tggcactagg caaggccttc aacatgccgg
      121 tcgaagccat ctcggccttc gacccctatt tccactatgc cgcgtttcac agcatctcgg
      181 gtgtcctcaa cgaagaagct ggaaaagtgt tccgtttcaa ggagcaggaa aagctccacg
      241 aagagatcat cgacagcggc ctcgcaaaga tctatcagtc gcacctcgac atctgccgcg
      301 agatcgctca ggctgagcaa accgacgtca agaccaccct gctggacggt aaggcctttg
      361 aaaaaatcat tcaatatgtg cgcaaggaca ttccggcatt gctgatcgtc ggacggatcg
      421 gcgtgcacag cgatgaggat atggatattg gcagcaacac agaaaatctc ttgcgatcgg
      481 cgccctgcaa tatcctggtg tcgaaccgga agtatgttcc accgatcgat acgctggcgg
      541 aatacacgat cgcctggacg gaagaagccc tccgtcggat ggaaaagatc ccgatattcg
      601 ccagaggcgt ggccaagacg gctatccacc gctatgcgat cgaaaagggc cataccatca
      661 tcagcaacac agtcgtcgat acggccatag gacacattct gccaaagggt gcgatggacg
      721 ccatgcgcgc actgggtggc aacttggatg ctgcgggtat cgaccgcaac aagatgcaag
      781 ctgatgagtc tgtcgtgaag gatcttatgg gctcgacgct cagcgggatg atgaccgaag
      841 ttgtggaaga aaaaccaacg gtcagtgcaa acacccaagc ctatttggac cggatgagtc
      901 aggattattt catctgcgac ggatgcggct acattggcaa gggcgagagg cctgtgaagt
      961 gccccgtctg tgcagccggg gggaccgctt caagctggtt gacaagtctc tcttcgaagc
     1021 agcggcgcat gctgaagggg ccatcgagac agacttggcc tacgatgatg tccccatgca
     1081 atggacgaag gatgctaaag aaggaatccg tgctgttcca gcaggctttc aacgccggcg
     1141 ggccaaagcc aagatcgaga agaccgcccg caaacttggc atgaccacga tcacgctgga
     1201 atatgccgca ccgatgattc aggaagcggc ttcagaagac tacaccccca tctttgcgaa
     1261 taagggaacc ggcgcctcga tggaagcaca ggctacggta gccgttgcgg caaatggcac
     1321 gaacggcgcg actgtcgccc atgagaacgg tcatgggagc gggaatggcc acgcggcaga
     1381 atctgctccc gcccctgcct ctccctatac ctggttgccg gaagctcaat cacggctcga
     1441 acgggcccca gaagggttca tgcgcgactg tacgcgtgcc ctcatcgaaa aacatgccga
     1501 taagctcggc gtcatggtca tcacggccga agtggcccac gagggcatcg aacaagctaa
     1561 ggattacatg gcggaagcca tgaagaccgg taacctcaag gacatgattg cgaacctgac
     1621 tggctcgtcg aagcccaagg ccggtcactg atgggccggt ctcttccggt tctcaacttc
     1681 ccggcactgg caggaacgct cggctctatc cagcaacagg tctcgcagtt ctttactccg
     1741 ccaaccactg ctcctgcacc gcacgacggc cgaacagtcg atgatttcaa accctatctc
     1801 atcgccctga atctcacgaa acgctgcaat cttaagtgcg accattgcta tcttgacgcg
     1861 acgacaaagt cggccggcgg aaacgacgaa ctctcgaccg aggaatgcta caagctcatt
     1921 gatcagattg ccgaagtcaa caggggttgc ctcctcgtga tcacgggtgg cgaaccgctc
     1981 gtccgtcctg acattctcga cattgcgcgc catgccgtga aactcgggtt catggtggtg
     2041 ttcggaacca acggcatgtt gatcgatgac cggatggcga aggatctggt ggacatcggc
     2101 gtgatgggaa tgggcatcag tatcgattcg ctggatcccc agaaacacaa tcgctttcgt
     2161 ggtgtgcctg gtgcgtggga agccgcactc gcaggcatcg aggcctgcaa acgaaacggg
     2221 ttggccttcc aagtgcactt cagtgcgcag cccatgaatt atcgagaact gcccgaggtg
     2281 atcgattggg cgcaccaact tggtgccaaa gtgctcaatg tttttttcat ggtctgtaca
     2341 ggccgtggtg aagaactgac cgacattacc ccagcgcagt acgaagaggt gctcggctac
     2401 ctggtcaact gtcaggacaa ttataaaggt atgctcgtcc gagcccgttg tgcgccgcat
     2461 ttcaaacggc tcgcctacga aaaggatccg aactccccta ttaccaaagc gacgggttat
     2521 atgggcggcg gctgccttgc cggcaccaac tatgcgcgcg tgacccctaa tggcgaactg
     2581 accccttgcc cctacatgcc cctgtctgcc gggaatatcc gcgagacaag ctttgtcgat
     2641 ctgtgggaaa agtcggatgt cttcaattcc ttccgctatc cgcaactgaa aggtaaatgc
     2701 ggcgattgcg agtacacaga catctgcggc ggttgccgtg cgcgtcccta tgtcgatcat
     2761 ggcgattggc tggacgaaga tcaatggtgt ctgtacaccc cgaagggtgg cgagaagatc
     2821 aaagttgcat ttaatacacc ggaagaaggc gacgtggcct gggatgagga ttcagaaaca
     2881 cgactcaacc ggattcccta tttcttgcgc gccatggtga agaaaggtgt cgaaaagcac
     2941 gcacgcgaga atggtatccc tctgattacc attgagttga tggaagaact ccgcaaaaaa
     3001 cgattcggaa acgacgcgcc agtcttcaaa ttctaacgtg atgagttaag attcattgat
     3061 ttcgcctgca cgcgatcttt gtttactgct catcacgagt tgcacaagtg gacgcccttc
     3121 aaagcctcgt taccgatcct cacacattca tcccaatcat caatcattgg cttcacttgg
     3181 tgtcggcggt catctggatc ggtggactgg cgttccttgt catcgccgta acaccaggac
     3241 tgaaggcggc ggtcgcgaag gaccaggtca agccaattac cgatgcgttt tatcaccatt
     3301 acaagaaagt cgctggcatt ctgctcatgg tcctgctgtt taccggcggt atcaatttac
     3361 actatgtcaa tcagatcgtc accgcgcaat ccggaagtgg gattcaacac catgcgacct
     3421 acctgatcgt cttctttatc aagctgtttc tcgtcatctg tcttctcacg ctctttctgt
     3481 acaccgtatt cttcaagtca gatgaggcag aagaaggtga tgaagagtcc tatgaggcaa
     3541 ttcccttcca acgagccgcg ctctggatcg gcttctttat cattctctgt gccgccgcga
     3601 tgaaacatct ccaccaatag attgcgtccc ctctttggtt ccctgtaaca tccatatagc
     3661 tcgtcgtatt gcttttgtcc ctgtatctcc taggataaag agcgtaccga atagaatacg
     3721 cctctttccc atagcaccaa ctccccccct tcattcacca acccatattt actcaacaac
     3781 ttatagcgta ggcttcggaa gtttttttca ctgatataac gctggtacag atgttgctgc
     3841 atttgcatta gctctctgat cactcgattc aagacgtagg aagctcatga tcacaagtga
     3901 gaagattctt ggcgaagcga aacgccggtg ggtccccact caggaggtta atacctctca
     3961 tgcaagtggg actacctctg gattgtgccc atctggagcc ctctcaccgc cacacgcttc
     4021 cgccatcagg gccgcgtcct gcctcgtagc agcggcgcgg ccctctaatt tggtcatgca
     4081 cgagttagtc cattggcggg gggatcgctt cattacagag aaactttgag agggatacaa
     4141 tgtcgctgac caatcaggcc gctccgagtc actcaatcaa aaatgagcgc cgcgagacca
     4201 ctagtacctc ttccagaccg aataaggtcg gacggcagca gccttggact gtccggctga
     4261 ccgtcagcct tcctggcg
//
LOCUS       DATIPF010000141         5482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_558400, whole genome shotgun sequence.
ACCESSION   DATIPF010000141 DATIPF010000000
VERSION     DATIPF010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558400"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VLL06_03675"
     CDS             <1..561
                     /locus_tag="VLL06_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSS30097.1"
                     /translation="TIILASTKFTQGAWIVFLLIAILVWMFRSIRSHYKAVSEQVALT
                     RDHRPPLPRRNIVLVPINGVNQAVIRAVDYARSRPGEVRAVMVDVDPQATARIQIQWA
                     QWGCGIQLTVLPCPYRSVLTSILDYVEDLLNKEQDTWVTVVIPEILPARWWHNILHNQ
                     RALMLKASLLFKDRVILTDVPFHLKR"
     gene            585..1631
                     /locus_tag="VLL06_03680"
     CDS             585..1631
                     /locus_tag="VLL06_03680"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HSS30098.1"
                     /translation="MKRHTYPWAYCLFMLFMLVMCLTVATPVHAFKITEPAEGAKLTA
                     GQTVTAHVDLDKDSGIVQVRYYWYGEQDDTLVEQEDATATGSIVAPVALIGLSSDRPA
                     FGGPLLVPKDGIGPMRLLAVAEISRGRLGTRSVFDEILVRVEPSASLIAIDFETDKPL
                     HLGRAGQSSAFGHVDSMGKVFELPVVGEFTDSVTRRISAVDSGTSYQSSNPKVIRVLS
                     SSLLQIVGNGKTTLTVSNRGKQASLDVTVDVNEEPNEPPVADAGPNRSVKAGTKVKLS
                     GLKSRDPEGEALYYAWSQVRGSKIPLLDVDNAEPSFLAPIVSEKRTYRFKLRVTDKKG
                     ADSVPAYVDVTVEP"
     gene            complement(1789..3354)
                     /locus_tag="VLL06_03685"
     CDS             complement(1789..3354)
                     /locus_tag="VLL06_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEXXH motif-containing putative peptide
                     modification protein"
                     /protein_id="HSS30099.1"
                     /translation="MPLLDVPLLARLQAEFRPSMKRLLDDLCLDLEGQYADVAESLGV
                     PVAYFRLLGHSLERDAYAHWKVVGWIEALNDLVYFIDLLQQIRGEENPRDFAAQLYTE
                     CEEKFFENSYLDDLFPRGVSQASGLNRRLNMLCARLTQELTQEALCLVPGLPMLWCAS
                     RRIPYWTVQIHLGNNVERAEIAGTMAIGLEGTTYEAPPPVKRVLKQFLGQPTISVRPQ
                     DLTLKIGRVVMSLCTMRGKRLEWSWMHRPPVVAMDTRFGAVTVGPTLVYGKDRQPRSV
                     TSTSANQVARIRRAWTIIQEAWPDGQEVLALLTARIIPLKAKGVVSFSYRHRPGLSFI
                     NCFDRDQLDLIDDLVHENSHHHLNLLLRKHVMYHRDRNQQIFYSPWRRSLRPLRGILH
                     AAFTFTMGVMLFERLSTWASGRGGSARWKQAGLTSRDLQRARFRCLEEVESVRYAIQD
                     LEYAGWHLKWLTGSGQRLVKQLAEAIEQIEHNITPHRKAVLASQFGPAIRKHVKELQQ
                     ARQTYGPVRLSKA"
     gene            complement(3605..4447)
                     /locus_tag="VLL06_03690"
     CDS             complement(3605..4447)
                     /locus_tag="VLL06_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30100.1"
                     /translation="MKIPSRTKILDLAAIPAGNRLGCVAVRVMTLSLVVAGSFLLQGC
                     LAGAWVALVAVDTMQSSNVTVGSFEQSWVEQAAVRSSNVTFGSFEQSWVAKQDQASDG
                     VFHSKVMSVAVLPVEGDQEMGARLATLLKQETTLRVESPANIAARVMTADSHSAESDD
                     VDRSALAKAVTRDLGVDTILVSRVAQSPSHPSDWGWKAEGSRRLYLYLMNRDGQLLWK
                     DELPFKLVQGSTPPVEASVQTDLSHHMMQHVKALRLDELGYLPNKEKRKKTNRPSATL
                     TPIE"
     gene            complement(4749..4976)
                     /locus_tag="VLL06_03695"
     CDS             complement(4749..4976)
                     /locus_tag="VLL06_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30101.1"
                     /translation="MQCTRCDGLMVTDNLIDIKESSIPMWMKGWRCVSCGNIVDPLIQ
                     QHRMIQQAGAMRLLTTETVVPRLRRTLKAIA"
     gene            5284..>5482
                     /locus_tag="VLL06_03700"
     CDS             5284..>5482
                     /locus_tag="VLL06_03700"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HSS30102.1"
                     /translation="MALTATEIALVMGELAPALAGSWIQKIYQPTDRLILLEMRAPGQ
                     TLRLLISCHQESARLHLTTSAF"
BASE COUNT         1239 a         1576 c         1461 g         1206 t
ORIGIN      
        1 accatcattc tggccagcac caaattcaca caaggcgcct ggatcgtctt cctcctcatt
       61 gccatattgg tctggatgtt ccgttcaatc cgctctcact acaaagctgt ctcagaacag
      121 gtagcgctca cgcgtgatca ccgccctccc cttccacggc gcaacatcgt cctcgtgccc
      181 attaatggcg tcaatcaagc agtcatacga gccgtcgatt acgcacgcag ccgtccgggc
      241 gaggttcggg cggtaatggt ggatgtagat ccccaggcta cggcacgcat acaaatccag
      301 tgggcccaat ggggctgcgg tatccagctg acggtgctcc cctgcccata ccgttctgtc
      361 ctcacttcta tcctcgatta cgtcgaggat ctcttgaata aggaacaaga tacctgggtc
      421 accgtcgtaa ttccggagat acttccggct cgctggtggc acaacatcct gcacaatcag
      481 cgggctctca tgctgaaggc ctccctcttg ttcaaagatc gtgtcatcct gaccgacgtg
      541 ccattccatc tcaagaggtg actgtgattc gggaaacgtc aagcatgaaa cgtcatacat
      601 atccttgggc ctattgcctg ttcatgctgt tcatgctcgt catgtgcctg accgtagcca
      661 ctcctgtcca tgcattcaaa atcaccgagc cagcagaggg agccaagtta acggccgggc
      721 aaactgtcac ggcccatgtg gacctcgaca aagattccgg catcgtccag gtccgctact
      781 attggtatgg tgagcaggat gacaccttgg tggaacagga ggacgcaaca gccacgggat
      841 cgattgtagc gccggtcgcc ctgatcggat tgtctagcga tcgtccggcc ttcggcggcc
      901 cgttactggt cccgaaagac ggcatcgggc ccatgcgttt gctggcagtc gccgaaattt
      961 cgcgcggacg attgggaacg agatccgtct tcgatgaaat ccttgtgcgc gtagagccct
     1021 cggcatccct catcgcgatc gatttcgaaa cggacaagcc cttgcatctc ggacgggccg
     1081 ggcaatcctc tgcattcggt cacgtcgatt caatgggtaa ggtcttcgag ttgcctgttg
     1141 tcggtgagtt cacggacagc gtgacccgtc ggatcagtgc cgtagacagc ggcacaagtt
     1201 accaatcttc gaaccccaag gtcatacgag tgctctccag cagcctgctg cagattgtcg
     1261 gcaacggaaa gacgaccctc actgtctcca atcgcggcaa gcaggcctcg ctcgatgtca
     1321 cagtcgacgt aaatgaggaa ccgaacgagc ctccggtcgc ggatgccggc ccaaaccgat
     1381 cggtcaaggc tggaacgaag gtgaaactga gcgggttaaa gagccgggac ccggaaggcg
     1441 aagccctgta ctatgcgtgg agtcaagtac gaggcagcaa gatcccacta ctggacgtag
     1501 ataacgccga accgtcgttc cttgcgccga tcgtgtcaga aaaacgcacc taccggttca
     1561 agttgcgggt gacggataag aagggggcgg attctgtgcc ggcatacgtc gacgtcaccg
     1621 tcgaacccta gcaggctgct gaaaaaggca cccaacttcg ttctcgtctc gaaaaaatcc
     1681 tcaacgtagc ccagaggcta cgcctccggt gtttttctcg cctgcggtct cgttggctga
     1741 tttttttgag cagcctgaat cagaaaatgc ttatccattc ttgaggcgtc acgccttgct
     1801 cagtcgcact ggcccgtagg tttggcgggc ttgctggagt tccttcacgt gcttgcggat
     1861 ggcgggaccg aattgggagg caaggacagc ttttctgtgg ggcgtgatgt tgtgttcgat
     1921 ctgctcgatt gcttctgcca actgcttgac cagccgttgc cctgagccgg taagccactt
     1981 gagatgccag ccggcatatt ccaaatcctg aatcgcatag cggacggatt ctacttcctc
     2041 aagacagcga aacctggctc gctgcagatc gcgcgaagtt agtcctgctt gtttccatcg
     2101 agccgaacca ccacgcccgg acgcccacgt cgacagtctt tcgaacagca tcacgcccat
     2161 cgtgaacgta aatgccgcat gcagaatacc tcgtagcgga cgcaggctcc gacgccaggg
     2221 agaatagaag atctgctggt tgcgatctct gtgatacatg acatgtttgc gcaacaagag
     2281 atttaaatgg tgatggctat tctcatggac caaatcgtcg atcagatcga gttggtcccg
     2341 gtcaaaacag ttgataaagg agagtcctgg cctgtggcgg taactgaagc tgaccacgcc
     2401 cttggccttg agcggaatga tccgagcggt caagagcgcg agcacctcct gtccatccgg
     2461 ccaggcctct tgtatgatgg tccatgcccg cctgattcgc gcaacctgat tcgctgaggt
     2521 ggacgtcacc gaacgaggtt gccggtcctt cccatagacg agcgtcggtc cgactgtgac
     2581 agccccgaat cgagtgtcca ttgccacaac cggcggccgg tgcatccaac tccactccaa
     2641 cctcttacct ctcatggtgc acagagacat caccacccgt ccgattttca gggttaggtc
     2701 ttgaggccga acagaaatag tcggctggcc aaggaattgt ttgaggactc tcttcaccgg
     2761 gggaggtgcc tcgtaggtag tgccctccag gccgatggcc atcgttccag cgatctctgc
     2821 ccgttcgacg ttgttgccga gatgtatctg gacagtccaa tagggaatcc tgcgagaggc
     2881 gcaccacagc atcggaagtc ccggtacgag acagagcgct tcctgtgtca actcctgagt
     2941 aagacgcgca cagagcatgt tcaatcgccg attgagcccc gaagcctgtg agacacctcg
     3001 tggaaacaga tcatccagat aactgttctc gaaaaacttc tcttcgcact cggtatacaa
     3061 ttgcgccgca aagtcacgag gattctcttc ccctcgaatt tgttgcagca gatcgatgaa
     3121 gtacaccaga tcgttcagtg cttcgatcca gccgaccact ttccagtgtg cataggcatc
     3181 tcgttcgagt gaatgcccga gcaaccggaa ataggccaca ggcacaccca acgattccgc
     3241 gacatcagca tattggcctt ccaaatccag acagagatcg tcaagcagtc gcttcatcga
     3301 cggacgaaac tctgcctgaa gacgagccag caatggcaca tcgagcaatg gcatggagga
     3361 cacatgctcc cagaagaaag agccagactg taccggagga gggacggcgc tgcaagaagg
     3421 agtagcagac cgcggaaaaa actatcgtta cacgctagaa tttcgagggt tcgcacctgt
     3481 ggcagaatgc cttaactgga agactgtcat aacctcactt acgctcaatg ggttccgaac
     3541 tgtagggccg cgaattccgg attgagctgg caggtgatac cgaacgacgg gttcgtgacg
     3601 atggtcactc gataggtgtc aatgttgcgg atggccggtt ggtttttttc cgtttctcct
     3661 tgttcggtaa gtaaccgagt tcatcaagac gcagggcttt gacatgctgc atcatgtgat
     3721 gggagagatc ggtctgcacg gatgcctcca ccgggggtgt ggaaccctgt accagcttga
     3781 agggcagttc atctttccac agtaactggc cgtcgcgatt catgagatac agatagagac
     3841 ggcgtgagcc ctctgccttc cacccccagt cacttggatg agagggagac tgggccacgc
     3901 ggctgacgag aatggtatcg acaccaaggt ccctcgtgac ggctttggcc agagcacttc
     3961 gatctacatc atctgattca gccgagtgag aatcggctgt catgactctt gctgcgatat
     4021 tcgccggcga ctcgacacgc aacgtcgttt cctgtttcaa cagagtggca agccgcgcac
     4081 ccatttcctg gtcgccttca accggaagca ctgcgacgct catgaccttc gaatggaaga
     4141 caccatcact cgcctggtcc tgcttcgcca cccaggattg ttcgaacgat ccaaacgtca
     4201 cgttgctgct tctcactgcc gcctgttcca cccaagattg ttcgaacgac ccgaccgtca
     4261 cattgctgct ttgcattgta tccactgcca ccaatgccac ccaggctcct gcgaggcaac
     4321 cttgcagcag aaaggacccg gctacgacca ggctgagagt catgacacgt accgcgacac
     4381 acccaagccg gttaccggct gggatcgccg ccagatcgag gatttttgtc ctagagggga
     4441 tcttcatata cgagataatt cagcaagagg gatgcctgac tagagggagc ctcgcacacc
     4501 gcctccaacc tgtcgagtta tttgctttta tcgacaggcc agcggaaata cgtttatctt
     4561 gttttaccga gcatggagtc tacgatttcc gctcattgaa caagatcgaa ccccaaaatc
     4621 tagggaggag aaagcgcatt gcaccaggat gttttggacg gagtagaaac cccattgaag
     4681 agatattggt agcagcgtaa ggatcggagt tcgtcgacct cgtcagaccg accggcgtca
     4741 acgcacaatt aggcaatcgc tttgagcgtc cgacggagcc tcggcacgac cgtttctgtt
     4801 gtgagtaacc gcatggctcc cgcctgttgg atcatccgat gttgctgaat gagcggatcg
     4861 acaatgttcc cgcaagacac acagcgccat cccttcatcc acatcggaat gctgctctcc
     4921 ttgatgtcga tcaagttatc ggtcaccatg agaccgtcac atctggtgca ctgcatgggg
     4981 gcctcctctg ttcatactga gcccacgact gcgacgttac acctattccc cttccaagct
     5041 tagcaggcgt gtggccagtc gcttacctta cgctgaaaga aagcaatcgc ggtgccacag
     5101 atgtcgttgc agctgcttgc gtgttttcgc ggtgaatcgt agagtgacgc cgaataacat
     5161 tggtcttaac cgcttctacc tgtggcacat tggtcccatg cggcaaaaat gacctggatc
     5221 gcgagcccga tgccctgagt ggggaaattc ccttcgaaga caacggctga tctgagacaa
     5281 attgtggcgc tgacagcgac agaaattgcc ctggtgatgg gtgaacttgc tccggccctg
     5341 gccggaagct ggattcaaaa aatttaccag ccgacggatc gcctcatttt actggagatg
     5401 cgtgctccgg gacagacact ccgacttctc atctcatgcc accaggaatc tgcccgtctg
     5461 cacctcacca catcagcctt tc
//
LOCUS       DATIPF010000142         3312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_27158, whole genome shotgun sequence.
ACCESSION   DATIPF010000142 DATIPF010000000
VERSION     DATIPF010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27158"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VLL06_03705"
     CDS             complement(<1..537)
                     /locus_tag="VLL06_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HSS30103.1"
                     /translation="MARRSATKKTVRSKKVSRTVLQQIRLFATDVDGVLTDAGMYYSE
                     TGDEWKKFNTRDGMGIKLLQRAGLITAIVTQERTKLVARRAEKLAIPELHQGVMDKLA
                     VIREMAVRHELSLQQVAYIGDDVNDLEALKAVGFSASPADGLPSIVAAVDYVCRKKGG
                     EGAVREIIEMILEAQGPSS"
     gene            complement(649..1500)
                     /gene="rfbD"
                     /locus_tag="VLL06_03710"
     CDS             complement(649..1500)
                     /gene="rfbD"
                     /locus_tag="VLL06_03710"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HSS30104.1"
                     /translation="MRILITGAQGQLGHALQQALSREDLILKDLPEFDLTQSESESQI
                     VAARPSVILHVGGYTNVDGAEREPDRALAVNARGTVFVARAAATLNARLIYVSTDYVF
                     DGTKATPYREEDVPHPINVYGQSKHEGEIAALTGCPDTLVVRTAWLYGHMGNNFVKTI
                     MRLASEKSFLEVVGDQRGCPTNADDLALVLKDLLTSDMRGICHVTNTGDCTWNEFAEA
                     IVRFMDLSTPVRPITTAQAGRLAKRPAYSVLAHGRLGTIRALLPHWQDALARFMRHAP
                     RLASAPS"
     gene            complement(1488..2516)
                     /gene="rfbB"
                     /locus_tag="VLL06_03715"
     CDS             complement(1488..2516)
                     /gene="rfbB"
                     /locus_tag="VLL06_03715"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HSS30105.1"
                     /translation="MRILVTGGAGFIGSHLVRRLVVQSGHEVVNLDALKYSGNRENLK
                     AIETHARHTFVHGDIGDQPLVERLIRTHRIEGVINAAAETHVDRSILDPGAFARTDVV
                     GTGVLLEEARRAGLQRFLQVGTDEVYGSVETGMSSETDRLAPRSPYSASKAGGDLLAL
                     SYWTTYRFPVMVTRGSNTYGPNQYPEKFVPLFVTNAIDNQSLPLYGDGRQCRDWLAVE
                     DHCSAIEHVFLRGAPGEIYNVGGGNERENIMVAELIVGHLGRSRDLIRFVQDRPGHDR
                     RYAVDCSKLRQLGWSPHVPFEEGLRATVQWYQEHEDWWRTIKSGEFRQYYEQQYRQRL
                     NQGTACGS"
     gene            complement(2566..3294)
                     /locus_tag="VLL06_03720"
     CDS             complement(2566..3294)
                     /locus_tag="VLL06_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HSS30106.1"
                     /translation="MRGVVLAGGLGTRLLPLTKVTNKHLLPIYDRPMIYYPIQALANA
                     GIQEIMLVTGGNSAGDFLKLLGNGKDFGLKRLNYSYQEGEGGIADALRLAEHFVDGEP
                     VCVILGDNIIEGNIARAAEAFGTQQQGAKILLKDVKEPQRFGVPILEGIRIVKIEEKP
                     ANPRSSYAVTGMYFYDAHVFDIIKTLKPSGRGELEITDVNNAYIADGTLTWDLLEGWW
                     TDAGTIESLHLANQLVAKTGANKL"
BASE COUNT          714 a         1018 c          840 g          740 t
ORIGIN      
        1 ggaacttgga ccttgcgcct ccaaaatcat ctcaatgatt tcccgaacgg ccccctcccc
       61 gcccttcttc cgacagacat agtcgaccgc cgccacaata gacggcagcc cgtccgccgg
      121 agaggccgaa aatcccacgg ccttcaatgc ttcaagatca ttcacatcgt ctccaatata
      181 ggccacttgc tggagcgaga gctcgtgtct gaccgccatc tcccgaatta ccgctaactt
      241 atccatcacc ccttgatgca actcaggaat cgcgagcttc tcggcacggc gcgccaccag
      301 ctttgtccgc tcctgcgtga caatggcggt gatgaggcct gccctctgca acagtttgat
      361 ccccattcca tcgcgcgtgt tgaacttctt ccactcatcg ccggtttccg aatagtacat
      421 tcctgcgtcg gtcaaaacgc cgtcgacgtc ggtggcaaac agacgtatct gctgaagtac
      481 ggtgcgggag accttcttgg atcgaacggt ttttttcgtt gctgatcgtc gtgccatcgt
      541 gtgaactcta agtttggtgt gttggcatat tactgatctt gcctagcgat gcccaccaaa
      601 aactgcgatt agggtacagc gatgggtcgg cgggtcggcc cgcacccact aactcggtgc
      661 ggacgcgagt cgaggagcgt gcctcatgaa tcgggcgagg gcatcctgcc aatgggggag
      721 caacgcgcgt atcgtaccca accggccgtg ggcaaggaca gagtatgccg gacgcttcgc
      781 gagacgcccc gcttgagcag tcgtaatagg tcgaaccggg gtggagagat ccataaacct
      841 gacgatggcc tctgcgaatt cattccaggt gcaatccccg gtattcgtca catgacagat
      901 tccccgcata tcactcgtga gtagatcttt caagaccagg gccagatcgt ccgcattggt
      961 cgggcaaccc cgctgatccc ccaccacttc gaggaacgac ttctcactcg ccaaccgcat
     1021 aatcgtcttc acgaaattat tccccatatg tccatagagc cacgccgttc gaaccaccaa
     1081 cgtgtcgggg caaccagtca atgcagcgat ttctccctca tgcttggatt gcccatacac
     1141 attgatggga tggggcacgt cctcttcacg atagggagtg gctttggttc catcgaaaac
     1201 atagtccgtc gacacataga tcagtctcgc gttgagcgtc gccgcggcac gggccacaaa
     1261 cacggtacct cgcgcgttca ccgccagcgc gcgatcgggt tcgcgctcag ccccatcgac
     1321 attcgtatag ccgccgacat gcaggatgac agagggccgg gcagcgacga tctgggattc
     1381 actctcagac tgcgtcagat caaactccgg caaatctttt aggatgaggt cttcccgcga
     1441 gagagcctgc tggagcgcat gacccaactg cccctgagca cccgtgatca ggatccgcat
     1501 gccgtgcctt gattgagacg ttgccgatac tgctgttcgt agtactggcg aaattcaccg
     1561 gacttgatcg tccgccacca atcctcatgc tcctgatacc attggacggt cgcacgcagt
     1621 ccctcttcaa atggtacgtg aggactccac cccaattggc gtaacttgct gcaatccact
     1681 gcgtagcggc gatcatgtcc cggccgatcc tgcacgaatc gaatgaggtc gcgggatctg
     1741 cccagatggc cgactatcag ttcagccacc atgatattct cccgttcatt acccccgccg
     1801 acgttgtaga tctctcctgg cgcaccacga agaaatacat gctcaatcgc cgaacaatgg
     1861 tcctccacgg ccagccaatc gcgacactgg cgtccatccc catagagagg aagagactgg
     1921 ttgtcgatcg cattggtgac aaacaacggg acgaattttt cgggatactg attcggccca
     1981 taggtattgc tgccccgggt taccattaca gggaaccgat aggtcgtcca atagctgagt
     2041 gccaagagat ctccaccggc tttgctggcg gaatagggac tgcgcggagc caggcgatcg
     2101 gtctcgctcg acatgccagt ctcgacgcta ccgtagactt cgtccgtgcc gacttgcaaa
     2161 aatcgctgca ggccggcacg ccgggcctcc tcgagcagca cgccggtccc caccacatcg
     2221 gttcgtgcaa aggcccccgg atcgagaata gaccgatcga cgtgagtctc tgccgcagca
     2281 ttgatgaccc cttcaattcg atgcgtccga atgagacgtt cgaccaaggg ctgatcgcca
     2341 atgtctccat gcacaaatgt atgccgggca tgcgtctcga tcgccttaag attttcacga
     2401 ttgccggaat atttcagcgc gtcgagattc accacctcat ggccactctg gacaaccaga
     2461 cgacgtacga gatgcgaccc aatgaatccg gcccctccgg ttacaagaat tcgcatctta
     2521 ggaccttatt gagttcgagg ttcggagttc atcgtggcgc gagtctcaca atttgttcgc
     2581 gccggttttc gcgacgagct ggttggccag atggagggat tcgattgttc ccgcgtcggt
     2641 ccaccagccc tccaacagat cccaggtcaa tgttccatcc gcgatatagg cattgttgac
     2701 gtcggtaatt tcaagctcac cccgccccga tggtttgagt gtcttgatga tgtcgaagac
     2761 gtgcgcatca taaaaataca ttcctgtgac cgcatacgac gatcggggat tcgccggttt
     2821 ctcttcgatc ttgacgatcc gaattccttc aagaatcggc accccgaacc gttgaggctc
     2881 tttcacgtcc ttcaagagaa ttttcgcccc ctgctgctgc gtcccaaatg cttcggcggc
     2941 acgggcgatg ttgccctcta taatgttgtc gcccaagatg acacacaccg gttcaccgtc
     3001 tacaaagtgc tcggccaatc gcagagcgtc ggcaataccg ccctctcctt cttggtagga
     3061 ataattgagc cgcttcagcc cgaagtcttt cccattgccc agcaatttca agaaatctcc
     3121 cgcgctgttt cctcccgtca ccagcatgat ttcttggatc ccggcattgg ccaatgcctg
     3181 aatgggatag taaatcatgg gccgatcgta gatcggcagc aaatgcttat tggtgacttt
     3241 ggtgagcggg agcaacctag ttcctagtcc accggccaga acaacgcctc tcattctcta
     3301 cacactcctc ct
//
LOCUS       DATIPF010000143         3796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_468774, whole genome shotgun sequence.
ACCESSION   DATIPF010000143 DATIPF010000000
VERSION     DATIPF010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468774"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /gene="ruvA"
                     /locus_tag="VLL06_03725"
     CDS             complement(<1..561)
                     /gene="ruvA"
                     /locus_tag="VLL06_03725"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HSS30107.1"
                     /translation="MIASLTGRLAFKAPTHLVLDVHGVGYEVLIPLSTYYGLPNLSES
                     TSLSVHTHVREDAIQLFGFLTSQEKDAFVLLTSVSGVGPKLALSLLSALPVPSLVSAI
                     RSGDVEKLTTVPGIGTKSAGRLVLELKDKVEKLHTGAASAHESPSQGQDAIFDDALSA
                     LVNLGYRSQDAKDALKQVRKATTESIV"
     gene            complement(619..1374)
                     /locus_tag="VLL06_03730"
     CDS             complement(619..1374)
                     /locus_tag="VLL06_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSS30108.1"
                     /translation="MGGHSHWATVKRHKGAQDVKRGKIFTRIIREVAIAARSGADPDG
                     NPRLRLAILKAKEANMPGDTLKKAIQRGTGELPGLVYEEFNLEAYGPGGTGILIEITS
                     DNRNRTVAELRNLLTKNHGNMAEAGAVSWQFHKKGLFTIEKSTVDEDTLLSLVLDAGA
                     EDVKVGEKSYEVITDPHYFEQVKKALVDSKIDTVIAEVGYVPQNTIRLEEKAAEHMLK
                     LMEVLDEHDDVQKVHANFDISDEVMEKVAAAAG"
     gene            complement(1506..2345)
                     /locus_tag="VLL06_03735"
     CDS             complement(1506..2345)
                     /locus_tag="VLL06_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30109.1"
                     /translation="MTARGLIRSAGWAFSLCNNPDGRARQRLLPSRGVVLVILAYLFL
                     SAATLWAQHDQHAQISMPEAPATGHEHHGAVDDQAMWEGSVAGIAYSEFNHHLTGMLV
                     LIIGFSELRQALAISFWAWTRFFLPGALLSAGFFLFVWSDHEAWPIGSLSFMQTFFGS
                     DSEIFQHKSYALLSLTVGTIELLRRLGHIGHAAWVAPLPLFAIIGGLMLFGHSHGVHP
                     SAEKIALDHAVMGTMAVTAGSCKLLSDWFRSPSNRQAARWELLWAGLLLLIGIQLMIY
                     AEL"
     gene            complement(2369..3100)
                     /locus_tag="VLL06_03740"
     CDS             complement(2369..3100)
                     /locus_tag="VLL06_03740"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HSS30110.1"
                     /translation="MKFVCLNCETYMNLEKVEKPEEGSLGVFFACPSCAAKFSMVTNA
                     GETGMMNALGLKLAPRAEAGASLDGLRALAGNGQTAPAVNSQTAVGPSVSTAMAPPAK
                     AGEKTSGCPFSAMVAEMGLTAGGKPGNAGTATTEFAWSADAKEKLDRLPSFVKPMVQS
                     SVEAFARKQGYQRITLQVMDDSKSESSHGMTWTPEAEKRLENIPDFIRPMARKEVERV
                     AKERGMAAITAQVMDDVKEKFLKFM"
     gene            complement(3518..>3796)
                     /locus_tag="VLL06_03745"
     CDS             complement(3518..>3796)
                     /locus_tag="VLL06_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HSS30111.1"
                     /translation="LVDTGLIVVSTTNPFGMAYVEAVQAIRTLVHPVPVIAVHMSKAP
                     EEVPPNTDIVLSGPADFDAATRRILEELKRRGVLAHAIGAKPTFQYSI"
BASE COUNT          874 a         1104 c          971 g          847 t
ORIGIN      
        1 cacgatggac tcggtcgtgg ccttcctcac ctgcttcaac gcgtctttgg catcctgtga
       61 acgatagccc aaattcacga gcgcagacag tgcgtcgtcg aagatcgcat cttgtccctg
      121 actgggcgac tcgtgtgccg aggcggcgcc agtatggagt ttttcgactt tgtccttgag
      181 ctccaagaca agacggccgg ccgacttggt accgattcct ggaaccgtcg tgagcttctc
      241 gacatctccc gaccgaatcg ccgagaccag ggagggcact ggaagagccg acaacagact
      301 gagcgccaac ttgggtccga caccggacac actggtaagg agcacaaacg catctttttc
      361 ctgtgacgtt agaaaaccga acagctgaat cgcgtcttca cggacatggg tgtgaacgct
      421 cagcgaggtg ctttcgctga gattcggtaa cccatagtag gtgctaagag gaataagaac
      481 ttcgtatccg accccgtgta catcgaggac cagatgggtc ggcgccttga aggccaatcg
      541 gcctgtcagt gaggcgatca tgcgatctca taccgtcagc ggacaaagta ccgtcaggca
      601 gcgcggctgc catacacctc atccagcagc agcggcgact ttctccatga cctcgtcgga
      661 aatatcaaag tttgcatgaa ccttctgcac atcatcatgc tcatccaaca cttccatcaa
      721 cttgagcatg tgctcggctg ccttttcctc taatctgatc gtattttgcg gaacataccc
      781 aacctccgca atcacggtgt cgatcttcga atcgacgagg gctttcttga cctgttcaaa
      841 gtagtgcgga tccgtgatga cttcatagct cttctccccc accttcacat cttccgctcc
      901 cgcgtcgagg acgagagaca gcagtgtatc ttcatcgacg gtgctcttct caatcgtgaa
      961 cagccccttt ttatggaact gccaggagac ggcgcccgct tccgccatgt tgccatgatt
     1021 cttcgtgagg aggtttcgta attcggcgac ggtgcgattg cggttgtcac tcgtaatttc
     1081 gatcaatatc ccggtcccgc caggcccgta cgcttccaga ttgaactctt catagaccaa
     1141 accgggcaat tctcctgtac ctcgctgaat agcttttttc agcgtatcac cgggcatgtt
     1201 ggcttctttt gccttcaaga tggccaaacg gagtctcgga ttaccatccg ggtcagcacc
     1261 cgagcgagcc gcaatcgcca cctcgcggat aattctggtg aagatcttcc cgcgcttgac
     1321 gtcttgagcc cctttgtgcc gtttgactgt tgcccagtga ctatgtccac ccatcacgta
     1381 ctccatgcag cgggcctcag tgaagccggc taccctaata gaccatctca ctcgctgaga
     1441 gtttgtactc gtagcacagg gtttggaaag gtgtcaatta tgccgggagg cgactgcgac
     1501 aagactcata actctgcgta tatcatcagc tgtatcccaa tgagtagaag caatcccgcc
     1561 cagagtaatt cccatcgggc agcctgccta ttggacgggg agcggaacca atcagatagg
     1621 agtttgcacg aaccggctgt gaccgccatc gtgcccataa ccgcatggtc caaggcgatc
     1681 ttctctgccg agggatgaac gccgtgcgag tggccgaaca acatcaagcc tccgatgatg
     1741 gcaaacaacg gcaggggtgc aacccaggct gcatggccta tatgtccaag tcgacggagc
     1801 agttcgatgg ttcccaccgt caatgacaag agtgcgtaac tcttatgctg aaagatttcc
     1861 gaatcactac caaagaacgt ctgcatgaag ctgagcgaac cgatcggcca ggcttcatgg
     1921 tcgctccaga caaagagaaa gaagcctgcc gaaagtaaag ctcccggtag aaagaatctg
     1981 gtccaagccc agaatgatat agccagcgcc tgacgcaact cactgaagcc gatgatcagt
     2041 accaacatcc cagtgaggtg gtgattgaac tcggagtagg cgataccagc gactgaccct
     2101 tcccacatcg cctggtcatc cacagcccca tgatgttcgt ggccagtggc cggcgcctcg
     2161 ggcatcgaaa tttgggcatg ttggtcatgc tgcgcccaca acgtcgcggc tgacaaaaac
     2221 aggtaggcca gaatgaccaa cacaacccca cgacttggga gcagtcgttg tcgggccctg
     2281 ccgtctggat tattgcataa agaaaaggcc cacccggctg accggatgag ccctcgtgcc
     2341 gtcatccaca tatgttcgcg atgtcaactc acatgaactt caaaaacttc tctttcacgt
     2401 catccatcac ttgagctgta atggccgcca tcccccgttc cttcgccaca cgctcaacct
     2461 ctttgcgagc catgggacgg atgaaatcgg gaatattctc aagccgtttc tcagcctcag
     2521 gtgtccacgt catcccgtga ctcgattcac tcttcgaatc gtccatcact tgtaatgtga
     2581 tcctctggta tccctgcttg cgagcaaagg cctccacact gctttgcacc atcggcttca
     2641 caaatgaggg aaggcggtcc agtttctcct tcgcgtcggc cgaccaggca aactcagtcg
     2701 ttgccgttcc cgcattgccg ggctttccac ccgcggtgag ccccatttca gcgaccatcg
     2761 cggagaacgg acaaccgctc gttttctcac cagccttagc aggcggcgcc atggctgtgg
     2821 agacagatgg tccaaccgca gtttgactat tcacagcggg agcggtttgg ccattccccg
     2881 ctaaagctct caacccgtcg agggatgcgc cagcttcagc ccgcggggcc agcttcaggc
     2941 ccaacgcatt catcatgccg gtttcgccgg cattggtcac catcgagaac ttcgcggcac
     3001 aggaggggca ggcaaaaaag acaccgagcg agccttcctc cggtttctcc accttctcga
     3061 ggttcatata ggtttcacaa ttgagacaga caaatttcat aaagactcct ctggcatcgg
     3121 cccacggtgg ttagtacaac ttttcgggcc tcgtcaacca attagcccat tcatacaggg
     3181 taacttgaac taagaaggga gttacgctat cacatggatt ttcaaggagt caaaccgctc
     3241 ggatggtggg gatcaagatg aataattcca cctggcgtta gggggttgag atagttatgt
     3301 gacgtatgcc cgggaaaacc tgcaagtatg gaacaagaaa ggacgcctac tgcaggatgc
     3361 tcaaaatttc gtccagcaag gtcgcagcga gcatccctca ttaacgggtg ggtgggatga
     3421 tcccaactgc gcgcgtcgaa cgagcacatt cctatcgggc gcgttccgcg agcaagggga
     3481 tcgtcccagc tacccgttcc tctttttcag catcctgcta gattgaatac tgaaaggttg
     3541 gcttggcccc aatcgcatga gccagaactc cacgacgctt gagttcctca agaattctgc
     3601 gagtagccgc atcaaagtct gccgggcctg acagcacgat atccgtattg ggtggaactt
     3661 cttccggtgc cttgctcatg tgaacggcga tgactgggac tgggtgaacg agcgtccgaa
     3721 tcgcttgaac cgcttcgaca taggccatgc caaatggatt ggttgtcgag accacgatca
     3781 atccggtatc cacaag
//
LOCUS       DATIPF010000144         4215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_27338, whole genome shotgun sequence.
ACCESSION   DATIPF010000144 DATIPF010000000
VERSION     DATIPF010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27338"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /gene="glmM"
                     /locus_tag="VLL06_03750"
     CDS             complement(<1..926)
                     /gene="glmM"
                     /locus_tag="VLL06_03750"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HSS30112.1"
                     /translation="MRKLFGTDGVRGVANLDPMTSEMAMQLGRAAAHLFMRRSGRHQI
                     VIGKDTRISGYMLESALTSGICSMGVDVLLVGPMPTPAIAFLTRSLRADAGVMISASH
                     NPYQDNGIKFFSNDGFKLPDEMESRIEELIVSNEIAHLRPTADAIGKAYRIDDAEGRY
                     IEFVKRSLPKELDFQGIKMVVDCANGAAYKVAPTVLRELGAKVDVIGNKPDGMNINSG
                     CGAVHPELLQKAVREQGAHLGLALDGDADRAIFVCEQGQVVDGDHIMAMLALDLHRQG
                     QLAKQTVVGTVMSNFGLELAMTGAGIVLVRTAV"
     gene            complement(919..1800)
                     /gene="folP"
                     /locus_tag="VLL06_03755"
     CDS             complement(919..1800)
                     /gene="folP"
                     /locus_tag="VLL06_03755"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HSS30113.1"
                     /translation="MLEPKLLPRQAKDRMIEIHAKPLIMGVVNVTPDSFFDGGRYVDV
                     AAAVAHAVRLVEEGADLLDIGAESTRPGADVVNEAEECRRAIPVVTAVVKAVTVPISI
                     DTSKAAVAREALDAGAILVNDVTALRGDPAMVDVVARTGAGIVLMHMNGTPRTMQQAP
                     YYNNVVGEISDFFEERIRFAMAHGIVRRQLILDPGIGFGKLLVHNLTLLAQLRYFLQF
                     ECPLLIGVSHKGFLGQLVDRPVQERQWATAAAVAMAVDRGAGILRVHDVKGMKDVVQV
                     AAAISQQTIESMKVHHA"
     gene            complement(1994..3805)
                     /gene="ftsH"
                     /locus_tag="VLL06_03760"
     CDS             complement(1994..3805)
                     /gene="ftsH"
                     /locus_tag="VLL06_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HSS30114.1"
                     /translation="MNSRTKNILFWVVVGLFMILLFNLFSVPHHEPEEDVIFSEFMTK
                     LDKGEVERVIIKASQISAVLKDKSRIRTYSVEYPELVQVLRDRGVQIEAKPPDESPWY
                     ITFLVTWGPFVLFLGLWFFLMRQMQMGGNKALSFGKSRARLLTEERKKVLFSDVAGID
                     EAKDEVLEIIEFLKDPRKFQKLGGRIPKGVLIVGPPGTGKTLLAKAIAGEAAVPFFSI
                     SGSDFVEMFVGVGASRVRDLFEQGKKHAPCIIFIDEIDAVGRLRGAGLGGGHDEREQT
                     LNQLLVEMDGFDTTEGVILVAATNRPDVLDPALLRPGRFDRQVVVNRPDLRGRTEILK
                     VHSKKVPIAADVELEKIARGTPGFSGADLENLVNEAALWAARLNKKEVAFVDFEMAKD
                     KVLMGAERKSLILSDEEKRTTAYHEAGHALIAKLIPGTDPVHKVTIIPRGRALGLTMQ
                     LPTDDRHGYSKVYLYNQLAILMGGRVAEELVLKDMTTGAGNDLERATDLARKMVCEWG
                     MSEKMGPLTFGKQNEQVFLGRELGSQRDFSDQIAMEIDEEVKRLVMENYERAKRLLTE
                     NMATLKRLAEALLEKEVLDSADIDNILMQPTSQAVPA"
     gene            complement(3907..>4215)
                     /locus_tag="VLL06_03765"
     CDS             complement(3907..>4215)
                     /locus_tag="VLL06_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HSS30115.1"
                     /translation="KVKMVTELTEDIKGKDVLLVEDIVDSGLTAQYLLKALGKKKPRS
                     IKICTLLSKPDRRTINVAIDYVGFKIPNRYVVGYGLDYQQKYRNLPYLAVLDVEGDQE
                     "
BASE COUNT          971 a         1261 c          999 g          984 t
ORIGIN      
        1 acggcagtcc ggaccaggac gatacccgct ccggtcatcg caagttccag tccgaagttg
       61 ctcataacag tccctaccac cgtttgtttc gccaattgcc cctggcgatg aaggtcgagt
      121 gccaacatcg ccataatatg atcgccgtcc acgacctgtc cctgttcgca gacgaaaatg
      181 gccctatctg catccccgtc caaagccaat ccgagatgag ccccctgttc tcggaccgcc
      241 ttctgtagca gctcgggatg gaccgcccca cagcctgaat tgatattcat cccgtcaggc
      301 ttattaccga tgacgtcgac cttcgctccc agttcgcgca gcacggttgg cgcaacctta
      361 taggcagcgc cattggcaca atcgaccacc atcttgatgc cttggaaatc cagctcctta
      421 ggaagagagc gcttgacgaa ctcgatgtaa cgaccctcgg catcatcaat tcgataggcc
      481 ttcccaatgg cgtcagcggt cggccgcaga tgggcaatct cgtttgaaac aatgagttcc
      541 tcaatgcgag attccatttc atcgggcagc ttgaacccgt cgttggagaa aaacttgatg
      601 ccgttatcct ggtagggatt gtgcgaggca gagatcatca cccctgcatc ggcacggagg
      661 ctcctcgtca ggaaggcaat cgccggcgtt ggcatcggac caaccagcaa cacatcgaca
      721 cccattgagc aaatccctga ggtcaatgcc gactccaaca tatagccgga aatgcgggtg
      781 tccttaccga tgacgatctg atggcgtccg gatcggcgca taaacaaatg tgctgcggct
      841 ctgcccagct gcatcgccat ttcgctggtc attggatcga ggttggcgac ccctcggacc
      901 ccatctgtac cgaataattt acgcatggtg caccttcata gattcgatcg tctgctggct
      961 aatcgcggct gccacctgca cgacatcctt catgcccttc acgtcatgga cacgaaggat
     1021 tccagcgcca cggtcgactg ccatcgccac cgccgccgct gtcgcccact ggcgctcctg
     1081 taccggacgg tcgacaagct gcccaaggaa ccccttgtgc gagactccga ttagcaatgg
     1141 acactcaaac tgcaggaaat agcgcaattg cgctaacagc gtaagattat gcaccagcag
     1201 cttaccaaac ccaatacccg gatcaagaat gagttgtctt cgtacaattc catgtgccat
     1261 cgcaaacctg attcgttctt caaagaaatc tgaaatctcg ccgaccacgt tattatagta
     1321 gggggcctgt tgcatcgtac gaggcgtacc attcatatgc atcaagacga tgccggctcc
     1381 agttcgagcg accacatcga ccatggcggg atcgcctcgt aatgccgtga catcattcac
     1441 aagaatcgcc cctgcgtcga gtgcctcgcg ggcaacggcg gccttggagg tatcgatcga
     1501 gattggaaca gtgacggctt ttacaactgc ggtcaccacg ggaatcgcac gacgacactc
     1561 ttctgcttca ttcacgacgt cagcaccagg acgtgttgac tctgccccga tatccagcag
     1621 atcagcccct tcttccacca accggacggc atgggccacc gccgccgcca catccacata
     1681 acgacccccg tcgaagaatg aatccggcgt cacattcacg acgcccataa tgagtggctt
     1741 cgcgtggatt tcgatcattc gatcttttgc ctgtcgtggc aatagtttcg gttctagcat
     1801 gaaaagatgg tgcctccata ccatggagag ccgaaactta acccaaggtc gctatctatt
     1861 attgggcctg tggagtacac gccacaacaa aacatcgcgc gagaaagact tccttctcgt
     1921 ccattatttt tcagttgtac tgcctgggac tccaatgccc tgaaatggtg tcccaggatc
     1981 agcgacggag aatttaggca ggaactgctt gggatgttgg ctgcatgagg atgttgtcaa
     2041 tatcagctga atcgagcact tctttttcca agagggcttc ggccaatctc ttgagagtgg
     2101 ccatattttc cgtgagcagc cttttcgccc gctcataatt ctccatcaca agccgtttga
     2161 cttcctcgtc gatctccatc gcgatttgat cgctgaaatc acgctggctc ccgagctcac
     2221 gtccaagaaa aacctgttcg ttttgtttgc caaatgtcag agggcccatc ttttcactca
     2281 tgccccattc acagaccatc ttccgtgcaa gatccgtcgc tcgctcaaga tcattcccgg
     2341 ctccggttgt catatccttc aagaccagtt cttccgcaac acggcctccc atgagaatcg
     2401 cgagctgatt gtacagatac actttcgagt agccgtgccg gtcatcggtg gggagttgca
     2461 tggtcagacc caaggctcgg cctcgaggaa taatggttac tttgtggacg ggatccgttc
     2521 caggaattaa cttggcgatc agcgcatgtc cggcttcgtg ataggccgtc gtccgctttt
     2581 cctcgtcgct caaaatcaga ctcttccgtt cagcccccat caacaccttg tctttggcca
     2641 tctcgaaatc gacgaatgcg acttctttct tgttgagtct cgcggcccag agcgcggctt
     2701 cgttgaccaa attttccagg tcggcacctg aaaatcccgg tgtgccgcgc gcgatttttt
     2761 ccagttccac atccgccgca attggaactt tcttcgaatg caccttgaga atttcggtac
     2821 ggccacgtaa gtccggtcga ttgaccacaa cctgccgatc gaaacgccct ggtctgagca
     2881 acgcggggtc aaggacatcc ggacggttcg tcgccgcgac caagatcaca ccctccgtcg
     2941 tatcaaatcc atccatctcc acgagcaatt gattaagggt ctgttcacgc tcgtcatgac
     3001 ctccacccaa ccctgcgcca cgaagccgcc caaccgcatc aatttcgtcg ataaaaataa
     3061 tacagggagc atgctttttc ccctgctcga agagatcgcg gacacgggat gctccgacac
     3121 caacgaacat ttccacaaaa tccgacccgc tgatgctgaa gaacggcacc gctgcctcac
     3181 cggcaatagc cttggccagg agcgtctttc ccgtaccggg aggaccgacg attaagacac
     3241 ccttggggat gcgtccgccg agcttttgga atttgcgagg atctttgagg aactcaatga
     3301 tctcgagcac ttcatccttg gcttcatcaa ttcccgcgac gtcggagaac aatactttct
     3361 tacgctcctc cgtcagcaga cgcgcgcggc tcttcccgaa tgagagagcc ttgttgcccc
     3421 ccatctgcat ttgccgcatc aagaagaacc acagccccag aaacagtacg aatggtcccc
     3481 aggtcacgag gaatgtaatg taccaaggac tttcatctgg tggcttcgcc tcaatctgta
     3541 cgccccggtc ccgcaagacc tgcaccaact caggatattc cactgagtag gttcgaatac
     3601 ggcttttgtc tttcagaacg gcactgatct ggctggcttt gataatgacc cgctcaacct
     3661 ctcctttgtc gagtttggtc atgaattcac taaaaatcac atcttcttcg ggctcatggt
     3721 gtggcacgct gaacagattg aatagcagaa tcatgaagag gccgacgacg acccagaaaa
     3781 ggatattctt tgtcctggaa ttcatccaga tctccttgtg gatggcacgc atacggtcag
     3841 aagaaccgtc agcgtgagtg atactaccac aggcgcattc attctgacaa ccgtgagacc
     3901 gagaccttac tcttgatctc cctccacatc caagacggcc agatagggaa ggttgcggta
     3961 cttctgctgg taatcgagtc catagccgac cacataccga ttcgggattt tgaatcccac
     4021 atagtcgatg gcaacattga tagtccgacg gtccggcttg ctcagcagag tacaaatttt
     4081 aatagaccga ggcttctttt tcccaagggc tttgagcaag tactgggccg tcagaccgga
     4141 atcgacgatg tcttccacca ataagacgtc tttacccttg atgtcctcgg tcagctctgt
     4201 caccattttc acttt
//
LOCUS       DATIPF010000145         3070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_294060, whole genome shotgun sequence.
ACCESSION   DATIPF010000145 DATIPF010000000
VERSION     DATIPF010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294060"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="VLL06_03770"
     CDS             <1..1196
                     /locus_tag="VLL06_03770"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSS30116.1"
                     /translation="KILQANTGEESFDQARAALFDDLPVAQPLARFKLDCANQGQAAL
                     ALVQLARDAGQPYAVAFVDMRMPPGWDGLETIEHLWNADAGLQVVICTAYSDQPWDQI
                     TQRIGQNDKLLILQKPFNHVEVLQLATALSRKWELGHAVAKQLKDLNRLVDDRTAELR
                     AANTQLTQTNLTLVRTVTDLEAAQAQILQQNDKLEQLASRDALTGCFNRRAFYAQFEK
                     AFAESREHGIELCCLMVDIDHFKRVNDQFGHALGDQAIQAVANGLNAGLRLSDAIGRY
                     GGEEFCLMFPRTTLAEASNLAERLRIQVEREAGDRVRAASRLVLTVSCGVSSIVFGAR
                     TPLELIDQADKALYAAKEGGRNCVMAIDPVIAGMNADRCEPQVRRITPRVSNLLVSPS
                     SPIED"
     gene            complement(1498..>3070)
                     /locus_tag="VLL06_03775"
     CDS             complement(1498..>3070)
                     /locus_tag="VLL06_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSS30117.1"
                     /translation="RVIGMLRRAYEPCLRWAIARPVWMVTPAVGLFVVSLAITAWLGI
                     EFVPRLDEGDMAIQVWRLPSVSLSESVRTALDVERVFRRFPEVVQVVTRTGSPEVATD
                     VMGVELSDVFVILTPQHEWTTASTREDLIAKMKPAILAEVPGVSLGFTQPIEMRFNEL
                     IAGTRSDLAVKIFGPDLEVLKQQAEAVARQLETVQGAADVKVEQVAGLPLLRVIVDRE
                     QIARYGLTADDVLTLVQTTRVGRVVGTVVQGPRRFDLVVRLADSALVNPLAVGNLLIP
                     TAQGELVPLSRVAAISVDTGPAQVSREHVQRRIVVESNIRGRDLGSFVAEAQRTVARE
                     VSLPTGYELTWGGQFQHLQEATSRLAMVVPITLLLILGVLSVIFGAMRPALLIFLNVP
                     LALSGGIVALWLRGLPLSISAVIGFIALFGIAVLNGVVLVSHIRTLEAEGLTTDQAVV
                     QGAMDRLRPVLMTALVASLGFLPMALATTMGAEVQRPLATVVIGGLITSTALTLVVIP
                     AMYARICRRLLSDYL"
BASE COUNT          710 a          887 c          860 g          613 t
ORIGIN      
        1 ggaaaattct tcaagcgaac acgggtgagg agagtttcga ccaagcgcgg gcagcgttat
       61 tcgacgatct gccagtcgcg cagccgctcg cacgattcaa actggactgt gccaatcagg
      121 gacaagccgc tctcgccttg gtgcagttgg cacgggatgc gggccagccc tatgcggtag
      181 cattcgtcga tatgaggatg ccgcccgggt gggatggctt ggaaaccatc gaacacctct
      241 ggaatgcaga cgcagggctt caggtcgtca tctgtacggc atactccgat cagccatggg
      301 accaaatcac ccaacgtatc ggccaaaacg acaagttatt gatccttcag aaacccttta
      361 accacgtgga agtattgcag ctggcgactg ctctctctcg aaagtgggag ctgggacacg
      421 cggttgcaaa gcagttgaag gacttgaaca gattggtcga cgaccgtacg gcggaactcc
      481 gagctgcaaa cacccaactg acgcaaacta atcttacctt ggtgcgaacc gtaacggatc
      541 ttgaggcggc tcaagcgcaa atattacaac agaacgacaa actggagcag cttgcatctc
      601 gagatgcgct caccggctgc ttcaaccgcc gcgccttcta tgcgcaattt gaaaaggcgt
      661 ttgccgagag tcgcgaacat gggatcgaac tgtgctgcct gatggtggac atcgaccatt
      721 ttaagcgcgt gaacgatcaa tttggacatg cgctggggga tcaagccatt caagcggtcg
      781 ccaacggcct caatgccggg ctccgattat cggatgccat cggccgctac gggggagagg
      841 agttctgttt gatgtttcca cgcaccacgt tagcagaagc gtcgaatctg gcggaacgtc
      901 ttcggattca agtcgaaaga gaggcgggag acagggtgcg agcggcatcc cgcctggtac
      961 tgacggtgag ctgcggcgta tcatctatag tgtttggtgc ccggactcct ttggaactaa
     1021 tcgatcaagc ggacaaggcg ctctatgccg ccaaggaagg tgggcgcaat tgtgtgatgg
     1081 ctattgaccc ggtgatcgcc ggcatgaacg ccgatcgctg cgagccgcaa gtcagaagaa
     1141 ttacaccccg ggtgtcgaac ctactggtgt ccccgtcttc accgatcgaa gattagcgac
     1201 ccgcaatggt aactggagac ttagccaatc aggaaagttt ttcagttgtt tgccaccact
     1261 ctgctaccgg aatgccctaa cccaggcgca ttcagtgcgt tctcgattac tccgattcct
     1321 tgttcttctg ctcagataca tgctcctgag tctgcggagt ttggtcaaca gggcatctcg
     1381 taatcagcgg gccgtaggtt cgacccctac acggcccacc aaaaatcaac cacttaccgc
     1441 ttcaactccc ctgccacccc taaaaggcag atggtcggtt tataggtctc ctgtcaatca
     1501 caggtaatct gagagcaatc ttcgacagat acgggcatac atggcaggga tgacgacgag
     1561 ggttaacgcc gtcgaggtga tgagtccgcc gatgaccacc gtggcaaggg gccgctggac
     1621 ctccgcgccc atcgtcgtgg ctaatgccat aggaagaaag ccgaggctgg ccacgagtgc
     1681 cgtcattaat acgggcctga gtcggtccat ggctccttgc acaacggcct gatcagttgt
     1741 gagcccttct gcttcgagtg tgcgaatatg gctaaccagc accacaccat tgagcacggc
     1801 aatgccgaag agggcaatga atccaatgac tgctgaaata ctgagtggca acccacgcag
     1861 ccagagggcc acgatgccac cggataatgc caaggggaca ttgagaaaaa tcagcaacgc
     1921 gggacgcatg gccccaaaga tgaccgatag gactcccaga attaagagga gcgtgatggg
     1981 cacaaccatg gccaatcggc tggtggcctc ttggagatgc tgaaactgtc cgccccatgt
     2041 caattcgtac ccggtcggga gcgatacttc gcgtgccacc gtgcgctgcg cctctgccac
     2101 aaagctgccg agatcccttc cacgaatgtt ggattccacg acgatccgtc gctgcacatg
     2161 ctcacggctg acctgcgccg gtcccgtgtc aaccgagatg gccgccacgc gggaaagagg
     2221 tacgagttca ccctgagcgg ttgggatgag cagattgccc actgcaagag gattcactaa
     2281 tgcgctgtcg gctaagcgga cgacgaggtc aaaccgtcgt ggcccttgca ccaccgtgcc
     2341 gacaacacgc cccacacgtg tggtttgcac aagggtgagc acgtcgtctg ccgtgagtcc
     2401 ataccgggcg atttgttccc gatctactat cactcgcagc agtggaagtc ctgcgacttg
     2461 ttcaactttg acgtcggctg caccctgcac agtttctaat tggcgtgcca cggcttcggc
     2521 ctgctgtttg agcacctcca ggtctggccc aaagatcttc acggcgagat cggagcgcgt
     2581 gcctgcgatc aactcgttaa atcgcatctc gatgggttgt gtaaagccca ggctgacgcc
     2641 aggaacctca gcgagaatgg cgggcttcat cttggcgatc aagtcttcac gagtgctggc
     2701 tgtcgtccat tcatgctgcg gcgtgaggat gacaaacaca tcggacagtt ccacacccat
     2761 cacgtcggtc gccacctcgg ggctcccggt tctggtcact acctgcacca cttcaggaaa
     2821 tcttcgaaat acccgctcaa catccaaggc agtccgaacc gactcactca gggacacgct
     2881 cggtaatcgc cacacttgaa tcgccatatc tccttcgtcg agacgaggca caaattcgat
     2941 gccgagccat gcagtgatgg cgagactcac aacgaatagc cccacggcgg gcgtgaccat
     3001 ccacaccggg cgtgcgatcg cccatcggag acagggctca taggcacggc gcagcatgcc
     3061 gatcacacgc
//
LOCUS       DATIPF010000146         4902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_469703, whole genome shotgun sequence.
ACCESSION   DATIPF010000146 DATIPF010000000
VERSION     DATIPF010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469703"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..432)
                     /locus_tag="VLL06_03780"
     CDS             complement(73..432)
                     /locus_tag="VLL06_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30118.1"
                     /translation="MIIVIFSLGLFTAWPTAATPPYTSIIIDGGSPYFVPKSATVSSG
                     APIRWENPTATEHTITHLGCLEDGSVCAFDSGIILPSGSFTLPGLAPGRYPYLCRIHP
                     IMHGTVTVSDSFVPSQL"
     gene            892..1371
                     /locus_tag="VLL06_03785"
     CDS             892..1371
                     /locus_tag="VLL06_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30119.1"
                     /translation="MAKNKKRPAKKTPAKAKAKTKAKSAVKKAVKKSASKKAAKKTTK
                     KIAKKPAAKKTAKKRVAAAPKAAQKKSAASALDRKSVKPFAGEDTQKSLLSQSMGSPM
                     LKDSDLDEELGEDEVDIAVEADEDVEEELTLDGEDEDAGFPEKPEGLLDDADEYHTH"
     gene            1510..1965
                     /locus_tag="VLL06_03790"
     CDS             1510..1965
                     /locus_tag="VLL06_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30120.1"
                     /translation="MSHFLFVAGALRERTSVDSAELQLGRGLWGLCTELIRTNLHTYL
                     DEKSRGLVYVLKVGIYAEFRMMPPVLDFSALDAFLSDELRTEARFGFVRIEEVRRLAC
                     SSVASQALLLNVLQIADQAELTRRLKLGMHRLTQAEYDAILQGAMGEAK"
     gene            complement(2092..2739)
                     /locus_tag="VLL06_03795"
     CDS             complement(2092..2739)
                     /locus_tag="VLL06_03795"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HSS30121.1"
                     /translation="MMPRHLVVTCTLIFILLCGLPASVPAAGIIELPVLAAIQQNNLG
                     VFEILMMWWDRKSEPNPVQLQWLLAGVRLGNDHLGAMAQAFAYAVERTPSIQHNGTVS
                     VQGVAYRPTGSDGPSAGAAMAVGFIALFKGEHIQRGIALTGTIEPGGQIGPVGSIPDK
                     IRAAAREGYRTVLVPRGQIHDARWNLIELGFQLNVIVKEVDTIDEAYQLMTGQRI"
     gene            2882..3943
                     /locus_tag="VLL06_03800"
     CDS             2882..3943
                     /locus_tag="VLL06_03800"
                     /inference="COORDINATES: protein motif:HMM:NF024965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HSS30122.1"
                     /translation="MMNQIGAWILVGGLAVLGLPLGAAGSNCPMETSAPGTGTTLTRQ
                     LSADCTEEEREARAVDATELLQAFKEGKGIDLSGVVVRGDLSLDTLPIGPLPPELEGM
                     KELQRLEVRVIPGSLIIVNSVVHGAVRHGSTQGLLVAKGPVKFSGTKFEQLVDLSRAV
                     FVQPVTMSGARFLRESYFVQGRFLRGMSAEKTAFGPHTRFHRSIFDGPVTFQQSGFNG
                     LAEFLEVVFEKDVDLSRTSFKLGTGFSGSRFQGLADFSDTSFDREAFFTFTQFDGDAD
                     FRRATFRSTADFSNASFKGRDDFSKASFAKGPQFTGAARSAIGESRPEPENPMTQYAI
                     GLSILTFIALLIVYLIRLR"
     gene            4023..4823
                     /locus_tag="VLL06_03805"
     CDS             4023..4823
                     /locus_tag="VLL06_03805"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HSS30123.1"
                     /translation="MDNSFDATDQTELPYQVRIQNFEGPLDLLLHLIRKSEINIYDIP
                     INLIAQQYLSYIEAMKDLNLTVAGEFLVMAATLLQIKSKMLLPADETTADEEDGPDPR
                     EELVRRLLEYKKFKDAARQLDEHERLWREVYSREQGPAIELESDETLLEHVSLFDLVD
                     VLQGILDRNPGKTLMEIVPDNLTVRERMNAILEMLEGQESVNFVELFEPSCHRLVIIV
                     TFLALLELMRLRTARVFQAEHFGPILVSRSFSLVPDPAEWDDAEWRGA"
BASE COUNT         1088 a         1186 c         1524 g         1104 t
ORIGIN      
        1 ctggttaagc gctcgcagcc tacacgacag gcaaggattc tcgcaaggag ctgaagtaga
       61 tagagccgga ggctagagct gcgaaggcac aaaggagtcg ctgacggtca cagttccgtg
      121 catgatgggg tggattcgac agagataggg gtagcgaccc ggagccaagc ctggaagtgt
      181 aaagctgccg ctcggcagga tgatcccgga gtcgaatgcg cagacgctcc catcttccag
      241 acagcccagg tgtgtaatcg tgtgttcggt agcagtgggg ttctcccatc tgatgggggc
      301 tccgctggat acggtggccg atttcgggac gaaatagggt gacccgccat cgatgatgat
      361 ggaggtgtaa ggcggcgtgg cggcggttgg ccaggctgtg aagagcccca gtgagaagat
      421 gacaatgatc atccgtaatt tcatcgtcgt gcaggcccat tgattgaacg gaacgtcatt
      481 ctaccacctg gtcaatgggg agagtaatgc ccccgggcag gattcttggg ttttgccctt
      541 gacgttgcag cctaattgta ggattctccc atccgctgga agtcacccat ggagagtcgc
      601 tgtgggaggt cctcacgtgc gtggcgctct gcccattcgg ctgctggtct ctggcgctct
      661 gtatggccgt gggaagcgca tcgaagccac agccagtatt gttactttgc cggcgtgtga
      721 accgaagtcg ctccgggtga cactcgatgc acatggcctg gggggcgggt gagaagccaa
      781 gatttttggc tgccagtgag ggggtgtgga ggcgttagtt gcaagaccct atgggttcat
      841 gctagatgga gaatgtgtca gacatctaac agctgagaga ggagaaattc gatggcgaag
      901 aacaagaagc ggccggcgaa gaagacccct gcgaaggcga aggcgaaaac caaagcaaag
      961 tccgcagtca agaaggcagt caagaaatcg gcttcgaaga aagctgccaa gaaaactacc
     1021 aagaagattg ccaagaaacc ggcagctaag aagacggcca agaaacgtgt tgccgcagca
     1081 cccaaggcag ctcagaagaa atcggcagcc tcagctctcg ataggaaatc cgtaaagccg
     1141 ttcgcggggg aagacaccca gaagtccctg ttgtcgcagt ccatggggtc gccgatgctg
     1201 aaagattccg accttgacga ggagttgggc gaggatgagg tggatatcgc tgttgaggcc
     1261 gatgaagatg tggaagaaga gctcacgctg gacggggaag atgaagacgc cggttttcct
     1321 gaaaaaccag aaggactgct agacgacgca gacgaatacc acacgcactg atactcgcac
     1381 gagatgtcta ttctgtacgc ctcggtcgag ttgtccggtc ggatgacact gtgagtctat
     1441 tccgccgcga tggcgtccgt tcccccatat gtcgcatcct cccgctgaat cctctgcgat
     1501 gaatgcccaa tgagtcattt tttgtttgtg gccggtgcct tgcgagaacg cacctcggtg
     1561 gacagtgctg agttgcaact cggtcgcggt ctctggggac tctgcactga gttgatcaga
     1621 acgaatcttc acacatatct tgatgagaag tcccggggac ttgtgtatgt gctgaaggtc
     1681 gggatctatg ccgagttccg aatgatgccg ccggtgttgg acttttccgc gttggatgca
     1741 ttcctcagtg atgagctgag aactgaggcc agatttggct ttgtgcgaat cgaggaggtg
     1801 cgccgattgg cctgttcgtc tgttgcgagt caggcgttgt tactcaatgt gctacagatt
     1861 gccgatcagg ccgaactcac tcgacgattg aaattgggta tgcatcggtt gacgcaagca
     1921 gagtacgacg caatcttgca gggcgcgatg ggagaagcga agtagcagga tgctcaaaat
     1981 ggccgttcag caaggccgca gcgagtcgaa accggaggcg taccctcggg ggtacgttga
     2041 ggatttcgat gagtcgagaa cgaagctggc ggactttttc agcatcctgc cttagattcg
     2101 ttggcctgtc atgagctgat acgcctcatc gattgtatcg acttccttta ctatgacatt
     2161 gagctgaaat cccagttcaa tgagattcca ccgcgcgtca tgaatctgcc cccgtggaac
     2221 taacacggta cggtaaccct ctcgtgcggc ggcacggatt ttatccggga tggagcccac
     2281 cggaccgatc tgtcctcccg gttcaatggt tccagtcaga gcaatgccac gttgaatgtg
     2341 ttcccccttg aagagggcga tgaatcccac ggccatggcg gcgcccgcgc ttggtccgtc
     2401 gctgcctgtg ggccgatagg ccaccccttg cacagaaacg gtcccgttat gttggatcga
     2461 gggcgtacgt tcgaccgcat aggcaaaggc ctgagccatg gcacctaaat ggtcgtttcc
     2521 cagccgtacg ccggcgagga gccattggag ttgaacggga ttcggttcag attttctgtc
     2581 ccaccacatc atcaggattt caaacacgcc gagattattt tgctgaatgg ctgccaggac
     2641 cggaagttcg atgatgccgg cggcaggaac agaggccggt agtccgcaaa ggaggatgaa
     2701 gatcaatgtg caggttacga caagatggcg tggcatcatg ggtgtcccag agaaccgtgt
     2761 ggagttggtg tgcgccggtg atgcatcatt tttcgaaagt gtagcaaatc cggttgggca
     2821 gtaaagcgag ttggcagttc atgccttgcg ttgggccagg gcattccgta taagggcgtg
     2881 catgatgaat cagatcggtg catggatcct ggtgggagga ctggctgttt tggggttgcc
     2941 gctgggcgcc gctggctcga actgtccgat ggaaacctct gctccaggga cggggacaac
     3001 gttgacgcgt cagttaagcg ccgattgcac ggaggaagag cgagaggctc gagcggtgga
     3061 cgcgaccgag ttgctgcaag cgttcaagga ggggaagggg atcgatctct caggggtggt
     3121 cgttcgtggt gatctgtctc tggacacact gcctataggc ccgttgccgc ctgagcttga
     3181 ggggatgaag gagctacaga ggcttgaggt acgcgtgatt cccggctctt tgatcatcgt
     3241 caattcggtg gtgcatggag cggtcaggca tggatcgaca caaggactat tggtggcgaa
     3301 gggacctgtg aagtttagcg ggaccaagtt tgaacagctg gtggatctct cacgggctgt
     3361 atttgttcag ccggtaacga tgtcaggcgc gcggttcctg cgggagagct attttgtaca
     3421 ggggcgattt ctgcgtggta tgtctgctga gaagacggca tttggcccgc atacgagatt
     3481 tcaccgatct atctttgacg gaccggtcac attccaacag tcggggttca acggactggc
     3541 ggagtttttg gaagtggtgt tcgagaagga tgtcgacctg tcgcgcacat cgttcaagct
     3601 gggcactggg ttttccggaa gtcgctttca aggcctggcg gatttctctg atacctcgtt
     3661 cgatcgcgag gcctttttca cctttaccca gttcgatggg gacgctgact ttcgccgggc
     3721 cacattccga tcgacggcag atttttccaa tgcgtcgttc aaggggcgag acgacttttc
     3781 caaagcctcg ttcgccaagg ggccacagtt taccggagcg gccagatcgg caatcgggga
     3841 gtcacggcct gagcccgaaa atccgatgac acagtatgcg attggcctct ccattcttac
     3901 gttcatcgct ctgctgatcg tctatctgat tcggttgcgt tgactagggt gttctctgtc
     3961 gtctaccaca actaatagat ggaggcttgc tccacctcac caagtctggt ataagatcgc
     4021 ttgtggataa ctccttcgac gctaccgacc aaactgaact gccctatcag gtccgtatcc
     4081 agaactttga agggcctctt gatctcttgc tccatctcat taggaagagc gagatcaaca
     4141 tttacgacat tcccattaac ctgatagctc agcaatacct cagctatatc gaggcgatga
     4201 aagacctgaa tctgacagtc gcgggggagt ttctcgtcat ggcggcaact ttgctccaga
     4261 tcaagtccaa gatgctgctt ccagccgatg agacgaccgc tgatgaagaa gatgggccag
     4321 acccccgtga ggagttggtg cggaggttgc tggagtataa gaagttcaaa gatgcggccc
     4381 gtcagttgga cgaacacgag cggttgtggc gggaggtgta tagccgtgaa cagggcccgg
     4441 cgattgagtt ggaatcggac gagaccctat tagagcatgt cagcctgttt gatttagtgg
     4501 atgtgttgca ggggatcctc gaccgcaatc ccggcaagac tctcatggag attgtgccgg
     4561 acaacttgac ggttcgggag cgaatgaacg cgattttgga aatgttggaa gggcaggaat
     4621 cggtgaattt tgtcgaactc ttcgagccgt cctgccatcg tctggtgatc attgtcacat
     4681 ttctggcatt gctggaactt atgcggcttc gaacggctcg ggtattccaa gcagaacatt
     4741 ttgggcctat tctcgtgtcg cgcagttttt cactcgtacc agacccggca gaatgggatg
     4801 atgctgaatg gaggggagca tgagctcagt tatggaacaa gaaacagacg agagagcccc
     4861 gattgggacc gcgtcggttc gtgaagacaa cggcgtaccg gg
//
LOCUS       DATIPF010000147         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_825124, whole genome shotgun sequence.
ACCESSION   DATIPF010000147 DATIPF010000000
VERSION     DATIPF010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825124"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VLL06_03810"
     CDS             complement(<1..654)
                     /locus_tag="VLL06_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HSS30124.1"
                     /translation="MFQTGVIGLVGSLGVVSKLVLFVLFIFSVISWAIIFFKWRAFRV
                     ADREDKQFLALLAKTRDLDELCRQVRRIDGSPSAVVFEGIMDRVGSGRLEERDGIGLG
                     PVDRHVIERTASHLGQRQLSQLETYLPFLATTGNITPFIGLLGTVMGIIDAFREIGTQ
                     GTASISAVAPGVAEALVATAAGLFTAIPAVIAYNYFLTRIRTTAFRLDSVSIEIMSSL
                     "
     gene            complement(729..805)
                     /locus_tag="VLL06_03815"
     tRNA            complement(729..805)
                     /locus_tag="VLL06_03815"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(769..771),aa:Asp,seq:gtc)
     gene            complement(929..1004)
                     /locus_tag="VLL06_03820"
     tRNA            complement(929..1004)
                     /locus_tag="VLL06_03820"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(969..971),aa:Val,seq:tac)
     gene            complement(1126..2043)
                     /gene="xerD"
                     /locus_tag="VLL06_03825"
     CDS             complement(1126..2043)
                     /gene="xerD"
                     /locus_tag="VLL06_03825"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HSS30125.1"
                     /translation="MMLDPLAERYLSHLRVEGGLSANTVDAYRRDLSKFHSYLHRKGI
                     VPLGPLTPETLTGFLRSLHEARLSRTSSVRCLSAIRGWLRFLVRERMIEESPTIGLTT
                     ISRGVRLPKTLPQEEVIALLELGAVPTLEDLRDRAMVELLYATGLRVSELVMAEVAQV
                     NLDVGYLRITGKGAKQRLVPMGEEARQLLQQYMGEARPQLLKKRASRYVFVSRRGGPL
                     TRQAFWKLLRCRARRAGITQVISPHMLRHSFATHLLEGGADLRSVQVMLGHTNIATTQ
                     IYTHIDRGRLKQVHDRYFPRIGQRRERKT"
BASE COUNT          584 a          718 c          600 g          517 t
ORIGIN      
        1 gagggatgac attatttcaa tggagaccga atccaaccgg aatgcggtgg tccgaatccg
       61 agtgaggaag tagttatagg caatgactgc gggaatggct gtgaacagtc cggccgccgt
      121 cgcgaccaag gcctctgcaa cgccgggagc cacagcggaa atactggcgg tcccttgggt
      181 cccaatctca cgaaacgcat caatgatccc catgacggtc ccgagcagtc cgataaacgg
      241 agtaatgttt cccgtggtgg ccagaaatgg aagataggtc tcgagctgtg agagctggcg
      301 ttgcccgaga tgggacgccg tccgttcaat tacatgtcga tcaaccggcc ctaagccgat
      361 cccatccctc tcctcaaggc gtccagatcc tacacgatcc ataattcctt cgaatacgac
      421 cgcgctggga ctcccgtcta ttcgtcgaac ttgccgacac agctcatcga gatctcgcgt
      481 ttttgcaagg agcgccagaa actgcttgtc ttcgcggtcc gcaactcgaa acgctcgcca
      541 tttgaagaaa ataatggccc atgaaataac cgagaagata aacaggacga agagcacgag
      601 cttcgacacg acaccgagcg aacccaccag tccaatcaca cctgtctgaa acatacggaa
      661 aggacctttc ccggctttag gaagtactca caattctgcc tacagagtct agcaaacaca
      721 gaagaaaatg gcggggccga cgggatttga acccgcgacc tccagattga caatctggcg
      781 tcctaaccag ctgaacgacg gccccttaaa actctacgta ttgcggaaga tgtggatgac
      841 aagactatat ctagtggctg gtcccgtcta aagtttcatt atttctgttc aatgatctgt
      901 cctgcccacc gtacaccagc cctcgcgctg gtaggcggaa cagggatcga acctgtgacc
      961 tctgccttgt aaaagcagcg ctctcccaat tgagctatcc gcctgatttc tttagcaacg
     1021 ttcgcggatg ctaccaactc cactcgccct tgtcaatcga atcccgcgct ctacctgtat
     1081 tttgagccat ggcacactcc gctcgtatgg aacgcgtctc tcccgttatg tcttgcgttc
     1141 tcggcgctgg cctatccgag gaaagtatct gtcatgcacc tgcttgaggc gtccccgatc
     1201 aatgtgcgta taaatctgtg tcgtcgcgat attcgtatgt ccaagcatca cctgaacaga
     1261 gcgaagatcc gcaccgcctt cgagcaaatg tgtcgcaaat gaatgtcgta gcatgtgagg
     1321 cgaaatcacc tgtgtgattc ccgcccgccg tgcacggcat cgcagcaact tccaaaacgc
     1381 ttgccgcgtc aatggcccgc ctcgcctcga cacaaacaca tatcgtgaag cccgtttctt
     1441 gagaagctgt ggccgtgcct cgcccatata ctgttgcaac agctgtcggg cctcctcgcc
     1501 catcggcaca agacgctgct tcgcaccttt gcccgtgatc cggagatacc cgacatcaag
     1561 attgacttgt gccacttcag ccatcaccaa ttctgatacg cgaagccccg tcgcatagag
     1621 caactccacc atcgcgcggt cacgcaggtc ttcgagagtt ggaaccgccc ccagttccaa
     1681 caaggcaatg acctcttctt gcggcagcgt tttgggcagt cgaacacctc gagatatcgt
     1741 ggtcaaacca atggtgggac tctcttcaat catccgttca cgcaccagaa accggagcca
     1801 tccacgaatt gccgagaggc agcgtacaga agacgttcga gacaaccgtg cctcgtgcaa
     1861 ggaacgaaga aatcctgtca acgtctccgg tgtcaaaggc ccgagcggta caattccctt
     1921 tcgatgtaag taggaatgga atttactcaa gtcgcggcgg taggcatcga ccgtattggc
     1981 agagagacca ccctccaccc gaagatgact caaatatcgt tcggccagcg gatcaagcat
     2041 catctgtgcg atgatagcca atcgatcaga tgaacacaac gaaagtgatc ggagtttacc
     2101 ttgacacccc cacagggttt ccgatagtag tcacatgcag atggcgtatt cgtcacaccg
     2161 cccaggagct catggccccc ctgttggtac ccccgtcttc tcatcgctcg tatagacatt
     2221 cggtacgctg gtaccatgtg ctgattcttg cttggagcat gctctggcca gtcactggca
     2281 tcgagttcac tccgctcgtc tgtgcagaaa cgggatccac gagtccggca aaccatcctg
     2341 cgcggaagca ggcgaagcgg ctgatcgatg cggatcaggc tcaagaagcc ctgaccatac
     2401 tccgtcggtt tctggcaac
//
LOCUS       DATIPF010000148         5984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_647478, whole genome shotgun sequence.
ACCESSION   DATIPF010000148 DATIPF010000000
VERSION     DATIPF010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647478"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1899
                     /locus_tag="VLL06_03830"
     CDS             157..1899
                     /locus_tag="VLL06_03830"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30126.1"
                     /translation="MTDDPKKEVPRYCAIPKLEWASRPDPTPVLLASGLEPAQVDEIL
                     QPYGFHRLRDADANLQSMAGDPHQRHQLAEILPALLEAIGRTADPDQALNHWERWLAS
                     GVSRSAVLEYLRSAPRMLDLVCAIFGNSDSLAFTLVRDPLLLYWLAQQNVLSTAPTRV
                     GMERTIRQSLEGVIATELKLDLLRRFRHREMLRIGVRDLVRLADVVETTSSLSDLASV
                     LIDAAYRIVDADLRARYGIPMHKNRQGRWVETGFTVIAMGKLGGHELNYSSDVDVLYV
                     CESHEGGTRPAASGGTKTKGPKQRRLSNEEYFEILARELTKALTEQTREGYVYRVDLR
                     LRAEGSVGQLTRSLDDYAKYYRTRGQVWERLALLKAWPIAGSKAVGRSFITMVRPFVL
                     APASKHLDVEQGLAIVEEVRSVKERIDAKMAERGHEQRNVKLGVGGIREIEFLVQTIQ
                     VLAGRRLPEVLGRGTLSSLVRLQKAGILSGKQQASLTRAYQLLRDVEHKLQMVHDLQT
                     HALPDQQKELERCAIRMGYDRADRPIALKQFQVDLAAHTTVVHDIFRSFFETPKTSVI
                     LKKTFRLMAQKTAK"
     gene            complement(1990..2346)
                     /locus_tag="VLL06_03835"
     CDS             complement(1990..2346)
                     /locus_tag="VLL06_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30127.1"
                     /translation="MIVEVESNENCEGSFFGRFKEVRPAQPVNQVRLYDRNPAGEWYW
                     ITGWSDDERTPTCQAYAQLVEDSGAGLTHLVYGGIHGLRFKPITIEEAWDLGSPHQWG
                     DAYLSLSSDRDLRYAD"
     gene            complement(2511..4148)
                     /gene="groL"
                     /locus_tag="VLL06_03840"
     CDS             complement(2511..4148)
                     /gene="groL"
                     /locus_tag="VLL06_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247953.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HSS30128.1"
                     /translation="MAKQLLYGDAARASILRGVNQLADAVKATLGPKGRNAILDKKFG
                     APTITKDGVTVAKEVELKNPYENMGAQLVREVASKTSDTAGDGTTTATVLAQAIYREG
                     AKNITAGANPMEIQRGINKAVEVVIGELKKLSKPCQNKTEISQVGTISANNDKTIGDL
                     IAEAMEKVGKDGVITVEEAKSMTTSLDVVEGMQFDRGYISPYFVTNAERMEAVMEDPL
                     ILISEKKISSMKDLLPVLEQVAKLGKPLVILAEDVEGEALATLVVNKLRGTLNVTAIK
                     APGFGDRRKAMLEDISILTGGQVISEDIGIKLENVKLTDLGRAKRITVDKDNTTIVEG
                     FGDSKKIEGRVKQIKAQIDETTSDYDREKLQERLAKLVGGVAVINVGAATETEMKEKK
                     ARVEDALHATKAAVEEGIVPGGGTAYLRCLGALDSLKLIAEQQVGVNIIRRALEEPIR
                     QIAVNAGMEGSVIVEKVRNDKNSNGGYNAATEEYVDMIKAGIIDPTKVSRCALQNAAS
                     VAGLMLTTEVMITELPEEKKEPAGGAGGHNHGMEGMY"
     gene            complement(4193..4501)
                     /locus_tag="VLL06_03845"
     CDS             complement(4193..4501)
                     /locus_tag="VLL06_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HSS30129.1"
                     /translation="MSTAAKEKTEKKDLAKGFQPLGDRVFVTYTEELDRTSGGIYVPD
                     SAKEKPQRGIVQAIGKKVEHVKVGDQVLFDKYSGSKLRIEDEECLILKEEDILGVFNS
                     "
     gene            complement(4762..5760)
                     /locus_tag="VLL06_03850"
     CDS             complement(4762..5760)
                     /locus_tag="VLL06_03850"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HSS30130.1"
                     /translation="MSPRKKDLDEQSIEPEILERSEGKIAEIGPPPEAPQPAIQGDHL
                     ATDITAVVPVTALQQYLAEVRRYPYLSKEEELHLFHEYRTHGNRDAAVKLIMANLRVS
                     ISIAAEYLHTGADHMDLIQEGNVGLMHAIKKFDPSKNVRFYAYAAWWSRAYILRYLLN
                     NYRLVKIGTTQDQRKLFYNLKKEKAKLEREGFAPDTKLLADRLNVRERDVIEMGQRLG
                     NWELSLDQPIGEDQEGSLLDVLPSQQVPADEQLAQTQLQTLFRTKLAEFIKTLEERDE
                     DILRNRILSETPLTLDDLGAKYSITKERTRQLEARIIKRLRDYIKKDIKDFEHLRI"
BASE COUNT         1152 a         1670 c         1696 g         1466 t
ORIGIN      
        1 cgaagtcccg gaggacctca tcatcgacgt cccgacacag ggaggccagg gtcttgataa
       61 tcgattcgcg tgatccaagc ccatccgtca tggcgggagt atagccgaag cgtggttcgt
      121 tgtaccagcg ccgatggatc agtataatgc ccgcctatga cagatgatcc caagaaggaa
      181 gtgccgcgct attgtgccat tccgaaactc gaatgggcct ctcgaccaga tccaactccc
      241 gtcctcttag cgtccgggct ggaaccggcc caggttgacg aaattcttca gccctatgga
      301 ttccatcgtc ttcgagatgc cgatgcgaat ctgcagtcga tggcgggcga tccacatcag
      361 cgacatcagt tggcagagat cctgcccgca ttgctcgagg cgattgggcg gaccgctgat
      421 ccagatcagg cgttgaacca ttgggaacgg tggctcgcga gcggcgtcag ccggtcggcc
      481 gtcttggaat atttgcgcag cgctcctcgc atgttggacc tggtgtgcgc gattttcggg
      541 aacagtgatt cactcgcctt cacccttgtt cgcgatcctc tgctcttgta ctggctggcc
      601 cagcagaatg tgttgtccac tgccccgacc agggtgggaa tggagcgcac gatccggcaa
      661 agtctggagg gtgtgatcgc cactgaactc aagttggacc tgctcagacg attccgccat
      721 cgagagatgc tgcgcatcgg cgtgcgcgat cttgtgcggt tagccgatgt cgtggaaacg
      781 acctcctccc tctcggatct ggcctcggtc ctcatcgatg cggcctatcg tattgtggac
      841 gcagatctac gggctcggta cggcattccc atgcacaaga atcggcaggg gcggtgggtg
      901 gagaccggct tcacagtcat tgccatgggg aagctcggcg ggcacgagct caattacagc
      961 tccgatgtgg acgtgctcta tgtctgcgaa tcccatgagg ggggaaccag gccggctgcg
     1021 tccggaggta cgaaaacgaa gggacctaag cagcgcaggc tctccaatga agagtacttt
     1081 gaaattctcg cacgggagct tacgaaggcg ctgaccgaac agacccggga aggatacgtc
     1141 taccgggtgg accttcgctt gcgcgcagaa ggttcggtag ggcaactcac tcgctcgctc
     1201 gatgactatg ccaagtacta tcggacgaga ggtcaggtgt gggaacggtt ggccttgctg
     1261 aaagcctggc ccatcgccgg gtcgaaggcg gttgggagat cgttcatcac catggtgcgg
     1321 ccctttgtac tggcgcccgc ctcaaaacat ttggatgtcg aacaggggct tgcgatcgtg
     1381 gaggaagtcc gatcggttaa ggaacggatt gatgccaaga tggcggaacg cgggcatgag
     1441 cagcgcaacg tcaaactcgg tgtcggcgga atcagagaaa tcgaatttct cgtccaaacg
     1501 atccaggtac tggccggccg gcgattgccg gaagtgttgg gccgggggac cctcagttcc
     1561 ttggtgcgac tgcagaaggc tgggatttta tcgggaaagc aacaggcctc tctgacccgt
     1621 gcctatcagc ttctccgaga cgtggagcac aaactccaaa tggtgcatga tctacaaacc
     1681 catgctcttc cggatcagca gaaagaactc gaacgctgtg caatccgcat gggctatgat
     1741 agagccgatc gaccgatcgc gctcaaacaa tttcaagtgg accttgccgc ccatacgacc
     1801 gtcgtgcatg acatctttcg gtcatttttc gaaactccta aaacgtcggt gatcctcaaa
     1861 aaaacgttcc gtttgatggc acaaaaaacg gccaagtagt tccagcgtaa cctgcagtat
     1921 tcgtgaacgc ttctgtgcgt tgagcctctg agcgcggtga gaatgccgct ggcggacttt
     1981 gcaatcttgt tagtctgcat accgcagatc gcggtcactc gagagtgaga gataggcgtc
     2041 tccccattga tgcggacttc cgagatccca cgcttcctcg atcgtgatcg gtttgaaccg
     2101 taagccgtgg atccctccat acactagatg agtcagaccc gcgccagagt cttcgaccag
     2161 ctgggcatag gcttggcacg tcggtgtccg ttcatcgtcg ctccagccgg taatccagta
     2221 ccattccccg gcgggatttc ggtcgtagag tcgtacttgg ttgaccggct gagcggggcg
     2281 cacttccttg aaccttccga agaagctccc ttcgcaattc tcgtttgact cgacttccac
     2341 aatcatagaa gacctcccga catattcaaa ccaggtagat ttatatcgcg cgcaatgtct
     2401 ccaatgattc taacgttcag taaaaacacg aagggcccgg cggtttcccg ccgagccctc
     2461 cgttggtctc cggtcgctga aagctgtgtg ctgtcagcta gagggttctt ttagtacatg
     2521 ccttccatgc cgtggttgtg tccacctgca ccaccggccg gctctttctt ctcttccggc
     2581 agttcggtga tcatcacctc ggtcgtgagc atcaatcccg ccacgctcgc cgcgttctgc
     2641 aatgcgcaac gcgacacctt ggtggggtcg atgatcccgg ccttgatcat gtccacatat
     2701 tcttcggtcg cggcgttata cccgccattg gagttcttgt cattgcgcac tttctcaaca
     2761 atgacggagc cttccatccc ggcgttcacc gcgatctggc gaatcggttc ttcgagcgcc
     2821 cggcggatga tattgacacc cacctgctgt tccgcgatca gcttgagcga atccaacgcg
     2881 ccgaggcaac ggagataggc tgttccgccg cccgggacga tcccttcttc gacggctgcc
     2941 ttggtcgcgt gcaacgcgtc ttcgacgcgc gccttctttt ccttcatttc ggtttcggtc
     3001 gcggcgccga cgttgatgac ggccacaccg ccgacaagct tcgcgagccg ctcttggagc
     3061 ttctcccggt cgtagtcgga ggtcgtctca tcgatctgtg ccttgatttg cttcacgcgg
     3121 ccttcgatct tcttcgaatc gccaaaacct tccacgatcg tggtgttgtc cttgtcgacc
     3181 gtgatgcgct tggcgcggcc aaggtccgtg agcttcacgt tttccagctt gatgccgatg
     3241 tcttccgaga tcacctggcc gcctgtgagg atcgagatat cctccagcat ggccttgcgg
     3301 cgatcgccga atccaggggc cttgatggcc gtcacgttga gcgtgccgcg gagcttgttc
     3361 accaccagcg tcgccaaggc ttcgccttcc acgtcttccg cgagaatcac gagcggcttg
     3421 cccaacttgg caacctgctc gaggaccggc agcaagtcct tcatgctgct gatcttcttt
     3481 tcggaaataa gaatgagcgg gtcttccatg acggcttcca tccgctcggc gttggtgacg
     3541 aagtaggggg aaatatagcc gcggtcgaac tgcatacctt cgaccacgtc caacgaagtg
     3601 gtcatggact tggcttcttc gaccgtgatc acgccgtctt tgccgacctt ctccatcgct
     3661 tccgcgatga gatcgccgat ggtcttatcg ttgttggctg aaatcgttcc gacttgggaa
     3721 atttcggtct tgttctggca gggcttgctg agcttcttca gctcaccgat taccacctcg
     3781 accgctttgt tgattccgcg ttgaatttcc atcgggttcg cgccggcggt gatgttcttt
     3841 gccccttcgc gatagatggc ttgtgcaagc accgttgcgg tcgtggtgcc gtcgccggcc
     3901 gtgtcgctgg ttttgctggc gacttcacgc acgagctggg cgcccatgtt ctcgtacggg
     3961 ttcttgagtt cgacttcttt ggccacggtc acgccgtcct tggtgatcgt cggagccccg
     4021 aatttcttgt cgaggattgc gttgcggccc ttgggaccaa gtgttgcttt cacggcgtcg
     4081 gcgagctggt tcacccctct gaggatggac gcgcgcgctg cgtcaccgta caaaagttgc
     4141 tttgccatag tcattccccc ttatgtcgtc ttgtggtgtg tagagatgat aattagctgt
     4201 tgaaaacgcc gagaatatct tcttccttga ggatcaagca ttcttcgtct tcgatccgga
     4261 gcttgctgcc ggaatacttg tcgaacagga cctggtcacc gactttcaca tgctcaacct
     4321 tcttgccgat cgcttggacg atgcctcgtt gcggcttctc ttttgcgctg tcgggcacat
     4381 agatcccgcc ggaggtccgg tccaattcct cggtgtaggt gacgaatacc cggtcaccca
     4441 gaggctggaa ccccttggct aagtctttct tctccgtctt ctccttggct gcagtgctca
     4501 taacaatacc ctcctggtga agttaacccc gtgaatatcg acgatataga gcaggttcga
     4561 actcgatggc gtaaccgtgg tactccagaa cgagcagaag tcctgatccg ctggacacgg
     4621 ggctaaagat agcctcgtgt tcgtccaagt caagggggag aaattgggaa attttccccc
     4681 gtcctcaatg cagggagatg gggaatcata cccgatcgag aagtcgatat accaggcagt
     4741 aattcggtgg gatcgaggat gtcagattcg taagtgctcg aaatctttga tatccttttt
     4801 tatataatcg cgcaaacgtt tgatgatacg ggcttccagt tgtctggttc gttccttggt
     4861 gatggagtat ttggcgccaa ggtcatccag cgtgagcggg gtttctgaga gaatcctgtt
     4921 ccgcagaata tcttcatccc gttcttcgag cgtcttgatg aattcggcaa gcttcgttcg
     4981 gaagagtgtc tgtaattggg tctgtgcaag ttgctcgtct gccggaacct gttgggatgg
     5041 aagcacatcg agaaggctcc cctcctggtc ttcgccgatc ggctggtcca gcgagagttc
     5101 ccagttgccc agccgctgtc ccatttcgat gacgtcccgt tcgcgcacgt tcaaacgatc
     5161 ggcgagcaac ttcgtgtctg gagcaaatcc ctcccgttca agcttggcct tctccttctt
     5221 gaggttgtag aacagcttcc gttgatcttg ggtcgtccca attttaacca ggcgatagtt
     5281 gttcagcaga tagcgaagaa tataggcccg agaccaccag gcggcatagg cgtaaaaacg
     5341 cacgttcttc gacggatcga acttcttgat ggcatgcatc aacccgacgt tgccttcttg
     5401 aatcaggtcc atgtggtcgg ccccggtatg gagatattcg gcggcaatcg aaatggatac
     5461 gcgcaggttg gccatgatga gcttcacggc ggcgtcgcgg ttcccgtgtg ttcgatattc
     5521 atggaacagg tgcagttctt cttctttgga gaggtaggga tagcgccgga cttcggcgag
     5581 atattgctgg agtgctgtaa cgggcaccac ggccgtgatg tccgtggcga gatgatctcc
     5641 ctgtattgcg ggctggggcg cttcgggagg cggaccgatt tcagcaatct tcccctcgga
     5701 tcgttccaag atctccggtt cgatcgattg ttcgtcgaga tcctttttgc gcggactcat
     5761 accggtcgag attatcataa gatcctctcg gctggatgct gaaaagttcg acggtatcgt
     5821 tcccttgcgt gaatgaaacg cggtccccta tagtgagtgg tccttgagta agctgcggga
     5881 tcggagaata tgatgaagga catgtttctc agcctcacac gcgggcattg gcccagtgtc
     5941 ataggcgcgt ggctccatct caccgtcagt tttatggtgt ggct
//
LOCUS       DATIPF010000149         3493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_470331, whole genome shotgun sequence.
ACCESSION   DATIPF010000149 DATIPF010000000
VERSION     DATIPF010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470331"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="VLL06_03855"
     CDS             complement(<1..1520)
                     /locus_tag="VLL06_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HSS30131.1"
                     /translation="MKLETHRSFPSELQSLVAAANAQLSASHAVERMWAHDYRLWKPD
                     PTDITNRLGWLTIIEYMKDRTEELRTFATSAKKRGIRDVVLLGMGGSSLGPEVLRTSF
                     GPSPGFPRLWVLDSTVPGWVRQVTQAIDPVRSLFILASKSGGTIEVMSLFAHFWELVH
                     HTKGNQGGEQFLAITDPGTGLEKLAAEHQFWRIFTNPPDIGGRYSVLSYFGLVPAALM
                     GVDVARLLTRATTMAQACRNQLSSGENPGADLGASMASLAQAGRNKVTLIASPQITTF
                     GLWAEQLLAESTGKEGTGLIPVANEPMVSPSAYGTDRLFIYLRLKGDENRQLDRHVAG
                     LARQGHPILTLALQDRYDLAAEFFRWEVATALAGYLLGIHPFDQPNVQESKDNTARVL
                     ETIQSTGRLPKQATATSAQAASQLKRQCHPGAYVAILAYTTPSSKMEQAIRSLRRALV
                     SHHHVTTTAGNGPRYLHSTGQLHKGGPTIGVFLQLVDPMIPDLSIPGKPFSFRTLA"
     gene            1800..1937
                     /gene="rpmH"
                     /locus_tag="VLL06_03860"
     CDS             1800..1937
                     /gene="rpmH"
                     /locus_tag="VLL06_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HSS30132.1"
                     /translation="MSFTSQQPSNLKKKREHGFRKRMSTKNGRKVLARRRAKGRARLT
                     V"
     gene            2108..2470
                     /gene="rnpA"
                     /locus_tag="VLL06_03865"
     CDS             2108..2470
                     /gene="rnpA"
                     /locus_tag="VLL06_03865"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HSS30133.1"
                     /translation="MPKGGGDRDLFLKRSRDIEYAKEHGRRISTALFNMVICRTDVGT
                     SRVGIVVGKRFGGAVSRNRVKRLFRELTRQLHGQLLTGHALVVFPKRDSIAQPFAVLK
                     EVWLSSLQRQQLLRIHLD"
     gene            2720..>3493
                     /gene="yidC"
                     /locus_tag="VLL06_03870"
     CDS             2720..>3493
                     /gene="yidC"
                     /locus_tag="VLL06_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HSS30134.1"
                     /translation="MDKRVVVFLVLSLGIILGFDVFLKQMGWFPEPPPAEESTATSPS
                     SPEQKPAPTSVEGQNSSPPGVRAPTQSGQKSNAPSSAPAPSILEQTVTVETDLVRVRL
                     SNRGGVIQSLELKRYHTSPPEVKPVQLVYQGGKFKGPLSITVANADIDKTIREGLYRI
                     EKDFTSLDAGHPVGHITMQFQDPITHLGVEKRLTFHHDSYVVDVAVALDGVNEPYEIG
                     LGTNFGIVEWGDGFIGLIGSASRVDDKIDKDTPEKELELK"
BASE COUNT          779 a          863 c         1043 g          808 t
ORIGIN      
        1 tgcgcgagcg tgcgaaagct gaatggtttt ccaggaatgc tgagatcggg aatcatcgga
       61 tcgacgagtt ggagaaatac tccgatcgtc ggccccccct tgtggagttg gcctgtcgaa
      121 tggagatagc gcggaccatt gccggctgtc gtggtcacat ggtgatggga tacaagtgct
      181 cttcggagcg aacgaatggc ttgctccatc ttggaggagg gagtcgtata ggccaagatg
      241 gcgacatagg cgccgggatg acactgtcgc ttgagttgtg aggcggcttg tgccgaggtc
      301 gcggtggctt gtttcggtaa ccggccagtt gactggatgg tttccaagac gcgggcggtg
      361 ttgtctttgc tttcttggac gttcggttga tcgaacgggt gaattcccaa gagatatcct
      421 gccagtgccg tggcgacttc ccatcggaaa aactctgcgg cgagatcgta acggtcttgg
      481 agcgcaagcg tcagaattgg atggccctgt cgtgcgagcc ctgcgacatg ccgatcaagc
      541 tgtctattct cgtctccctt cagccgcaaa tagataaaga gccgatccgt gccataggcg
      601 cttggtgaga ccatcggttc attggccaca gggatgaggc cggttccttc cttacccgtg
      661 ctttctgcaa gaagctgttc cgcccacaga ccaaacgtag tgatctgtgg tgatgcaatc
      721 agggtgacct tgttgcgtcc tgcttgtgcc aaggaggcca tcgatgcgcc gaggtccgcg
      781 ccggggtttt ctccagaaga aagctggttt cggcaggcct gggccatggt tgtagcgcgc
      841 gtgagtaatc gtgcgacatc aacccccatc agggcggcag ggactaatcc aaagtaggag
      901 aggaccgaat accgtccacc gatgtcaggg ggattcgtaa agatgcgcca gaactgatgc
      961 tctgccgcca gtttttccaa ccccgtgccg ggatcggtaa tggcaagaaa ttgttcgccg
     1021 ccttggttgc ctttagtgtg gtgaaccagt tcccagaagt gggcaaagag ggacatgact
     1081 tcgatcgttc caccggactt gctggcaagg atgaacaagc ttcgaaccgg atcgatcgcc
     1141 tgggtgacct gtcgaaccca gcctggaacg gtggaatcaa gaacccacag gcgtgggaac
     1201 ccgggggatg gtccaaatga tgtacgtagc acttcaggtc ccaggctgct gccacccatt
     1261 ccgagcagga ccacgtcacg gatgccgcgc tttttggccg aagtggcgaa ggtccgcaac
     1321 tcctcggttc gatccttcat gtactcgatg atggtcaacc aaccgaggcg gttggtgatg
     1381 tcggtgggat ctggcttcca gagtcgatag tcgtgggccc acatccgctc gaccgcatgg
     1441 gacgcggaga gttgtgcatt cgccgcagcg accagggatt gaagttccga gggaaacgat
     1501 cgatgagtct caagtttcat ggcaagcctc tcttctattc taattatcgg ttgcgagaaa
     1561 cacaaacctg agatctcgaa tatggcgttg tcgttcatta aaaatggata aaaggaacag
     1621 gcattctgtg aatgtccagg ttggagtagg cctatcaggc gagaaagagg ataggatgga
     1681 ggagaattgg gcctgtctcc tgtcaaatga acaatggacg gtacgtcgtt tgacagggtg
     1741 acaccccgtc cctataatga ccagttattc gtacggcttc ttaggctagg agtgatctta
     1801 tgtcgtttac atctcagcaa ccatcgaacc tcaagaaaaa acgtgagcat ggattccgca
     1861 agcggatgag tacaaagaat ggccggaagg tcttggctcg tcgtcgtgca aagggacggg
     1921 cccgcttgac cgtataggtc gagccacgct ggctagggct tcacgaagga atcttcgccc
     1981 tccgttacgt cccccagact cggtagctcc cagcacagga tgacgttgtg tggtcatgac
     2041 cgagtccgga aatcggagct cgaaggggat ggcctgtgca aactcctcct ctcccaggat
     2101 agtatcgatg ccgaagggtg gaggtgacag ggatctgttt ttgaaacgca gccgcgacat
     2161 tgagtatgcc aaggagcacg gtcgtcgtat ctcaactgct ctgttcaata tggtgatttg
     2221 ccggactgac gtgggtacca gccgggtcgg tattgtcgtg gggaagcgat tcgggggtgc
     2281 agttagccga aatcgggtga aacggttgtt tcgggaactc actcggcaat tgcatggtca
     2341 attgctcact ggacacgctc tggtggtctt ccccaaaagg gattcgattg cgcaaccctt
     2401 tgcggtgttg aaagaggtgt ggctgtcatc gctccaacgc caacagttgc ttcgtatcca
     2461 tctagactag cgtgggctcg tgctaccttt cgatgcttgc cgctatttat ggtggaattc
     2521 gtcactgctt gcacagcctg ccagtttgag cccagctgct ctgaattggc gagtgagtcg
     2581 atcaatctgt atggggcact agggggaaga caaacgctca tgatgtgcca tcttcctaat
     2641 ctggagctca tcgaagtgca gaggaatggt tcttcagtag caggccctct attgtttaca
     2701 tcggtgtagc ggtttattta tggacaaacg tgttgtcgtc tttctggttt tatcgttggg
     2761 gatcatcctg ggatttgacg tgttcctcaa gcagatgggg tggtttccag aacctcctcc
     2821 tgccgaggaa tccacggcta caagcccctc ttcccctgag cagaaaccag ctccgaccag
     2881 cgtggaagga cagaacagca gcccaccggg cgtgcgcgcc ccaacccaat ctggtcaaaa
     2941 gtccaacgct ccttcatcag ctcctgcacc atcgatattg gaacagacgg tcacggttga
     3001 aacggatctc gttcgggtta gattgtcgaa tcgtggtggg gtcattcaaa gtctggaact
     3061 gaagcgctac catacgtccc ctcctgaagt gaagccggtt caactcgtgt atcagggcgg
     3121 aaaattcaaa ggcccactct cgattacggt cgcgaatgcc gacatagaca agacgattcg
     3181 agaggggctc taccgaattg aaaaagactt cacaagccta gatgccggtc atccggtcgg
     3241 gcatatcacg atgcagttcc aggatcccat cacccatctg ggcgtggaga aacggctgac
     3301 gtttcatcac gatagttatg tggtggatgt cgcggtcgcc ttggatggcg tgaacgaacc
     3361 ctatgaaatt gggttgggga cgaacttcgg tatcgtagaa tggggagatg ggtttatcgg
     3421 tctgatcggg tcggcttcgc gagtagacga caaaatagat aaagacacgc cggagaagga
     3481 acttgagctg aag
//
LOCUS       DATIPF010000150         1955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_470882, whole genome shotgun sequence.
ACCESSION   DATIPF010000150 DATIPF010000000
VERSION     DATIPF010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470882"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VLL06_03875"
     CDS             complement(<1..224)
                     /locus_tag="VLL06_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30135.1"
                     /translation="MLLKVFGDKSSLGKAAADQAATAIRRAIASRGEARIIAATAASQ
                     LEFLDALTKEPGIDWTKVEAFHLDEYIGLP"
     gene            409..>1955
                     /locus_tag="VLL06_03880"
     CDS             409..>1955
                     /locus_tag="VLL06_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HSS30136.1"
                     /translation="MKRFEGVLRLFILTVAVVAMACVCFAQTVTVDATPGHEVNRFSP
                     LRALGSTVDRVPSNATDVFFRPDQIKKVLSAGWGTITYRQNTDLFVQAWHWNPKGTWS
                     DPTGKGYFTGDANPTSEMIRHSYGYSLPHRGFTRNGGSEFDGYSRLDDGDAESYWKSN
                     PYLTKEFTGEDDSMHPQWVVIALDKKEDVNAIRIQWAEPYARDFEVQYWEGDGDAMDE
                     QDKGSWKTFPSGAFTAGSGGDATLSLATSPISTKFVRVWMTRSSNTCDSHGSADRRNC
                     VGYAIRELSLGTKNETGQFKDLIHHSPDQKQTLTLCSSVDPWHESSDLYVAPDRMESG
                     DQPGFDLFFTSGITRGLPAIIPVAMLYSTPEDAAAQMSYIKKRGYPVLYIEMGEEPDG
                     QYMQPEDDAALYLQFATALHRVDPNFKLGGPVFEGVSEDIKAWADGQGRTSWFGRFLD
                     YLKSHGRIQDLAFMSFEHYPYDGCETPWENLYQEPELITHIMKVWRDDGLPAGIPLLD
                     TETNAHGG"
BASE COUNT          456 a          482 c          575 g          442 t
ORIGIN      
        1 atcgggagtc cgatgtactc gtcgaggtgg aaggcctcga cttttgtcca gtcgatgcct
       61 ggttctttgg ttagcgcatc gaggaattcc aactgggagg ctgcggtggc agcaataatg
      121 cgagcctctc cccgagaagc aatggcacgt cgaatggccg ttgcggcctg atctgccgct
      181 gccttgccca atgacgattt gtcgccgaat accttgagga gcatttgcga agtccgggga
      241 aaaagattga agaccaaaaa attatacaaa aagggacaac gaactgagga cggtgcggac
      301 cagagtttca tcgaatgaaa acccggtgcg tagtcatggc gtgtgacggc gagttgttgt
      361 atgacagctc ttgctgagtg aagtgccaac gctctaatga gatcaattat gaagagattc
      421 gaaggagttc tcagactatt tattctgacc gttgcggttg tcgctatggc gtgcgtgtgc
      481 tttgcgcaga ccgtgaccgt cgatgcgact ccgggccacg aggtcaacag gtttagtcct
      541 ttacgcgctc tgggttcgac ggtcgaccgt gtgccgagta atgcgaccga tgttttcttt
      601 cgtcccgacc agattaaaaa ggtcctctcg gcgggatggg gaactattac ctaccgtcag
      661 aacacggatc tctttgtgca agcatggcat tggaatccta aggggacctg gagcgatccg
      721 actgggaaag ggtatttcac gggtgatgcc aatccaacca gcgagatgat tcgtcactcg
      781 tacggctact cgctgccgca tcgtggattc acgcgcaacg gagggagtga gtttgacggc
      841 tactcgcggc tggacgatgg agatgctgag tcctactgga aaagcaatcc gtacctgaca
      901 aaagaattca ccggcgaaga tgactccatg catccgcagt gggtcgtgat cgcgctggac
      961 aagaaggaag acgttaacgc gattcgtatc caatgggccg agccctatgc gcgcgacttc
     1021 gaagtgcagt attgggaagg agacggcgat gcgatggatg agcaggataa aggatcgtgg
     1081 aagacgttcc catcgggagc attcactgcc gggagcggtg gcgatgcaac tcttagtctc
     1141 gcgacatcgc cgatcagcac aaagtttgtg cgcgtctgga tgacccggtc gtccaatact
     1201 tgcgacagtc atggttcggc ggatcgccgc aactgcgtcg gctatgctat ccgcgaattg
     1261 tccttgggta cgaagaacga aacgggccaa ttcaaagacc tcatacatca ctcgccggat
     1321 cagaaacaga cgctgactct ttgttcttcg gtcgatcctt ggcatgagtc ttcggatctg
     1381 tacgtcgctc cggatcgtat ggaatcgggc gatcagcccg gtttcgatct tttctttacc
     1441 agtggaatca cccgaggatt acccgcaatt attccagtgg ccatgctgta tagcacaccc
     1501 gaagatgcag ctgcacagat gtcctacatc aagaagcgcg gctatcccgt gttgtacata
     1561 gaaatgggag aagagccgga tgggcaatac atgcagcccg aggatgatgc ggcactctac
     1621 ctgcagtttg caacagcgct tcatcgcgtg gacccgaact tcaagctagg tggtcccgtc
     1681 tttgagggag tttctgaaga tataaaggcc tgggccgatg gccagggacg gacatcatgg
     1741 ttcgggcgct ttcttgacta cctgaaatcg catgggcgaa tacaggacct cgcattcatg
     1801 tcgttcgagc actatccgta tgacggttgc gagacgccct gggagaatct gtatcaagag
     1861 cccgagttga taacccacat tatgaaagtc tggcgcgacg acggtttgcc tgcggggatt
     1921 ccgctgctcg atacggaaac gaatgctcat ggtgg
//
LOCUS       DATIPF010000151         7680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_382999, whole genome shotgun sequence.
ACCESSION   DATIPF010000151 DATIPF010000000
VERSION     DATIPF010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382999"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="VLL06_03885"
     CDS             complement(<1..1147)
                     /locus_tag="VLL06_03885"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSS30137.1"
                     /translation="MPHKQVVIIGGGFGGLTAAQSLRDVDVVLIDRTNHHLFQPLLYQ
                     VATAALSPGDIAWPLRTIFRPYHNVRVVMDEVLSIDRAARLVRLRDSAPIAFDALIVA
                     PGSRHAYFGHDSWEQSAPGLKTLADAIYLRERMLLAFEEAERRRIATGTRDGLTFVIV
                     GGGPTGVELAGALAEIGRKAMGPDFPNLRLNDLAILLVEGGDRILSGFSADLSAKAAT
                     ALERKGVTIRLNSQVSEVRADGVMIGSQFVRSTNIIWAAGNRASPLLNSLAVPQDKSG
                     RIQVRADLTIPGESWIFVIGDSAHCVDPNGKPLEGLAPVAVQQGRYVARLIREETAPD
                     QRRPFVYADRGTKRHEDSGSRRKSEPGGRLCAVTGQAGSWCLGRRVKL"
     gene            complement(1285..2073)
                     /locus_tag="VLL06_03890"
     CDS             complement(1285..2073)
                     /locus_tag="VLL06_03890"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HSS30138.1"
                     /translation="MKIRVLGCHGSDQLLDGNGRTMQCRPCAFLINDTVLVDAGTVGS
                     RLTLPEQTKIRHVLLSHLHFDHIKDLPTLADNLAGETGSSLVVASIPEVLDGLRAHVF
                     NSSVYPDFFRLPDADRPVLVSEKLRLNCEWMAAGLRIVPVRVNHLVPTVGFLISDEHT
                     TILYSGDTYQTEDLWRAASSMPSLKAAFIEVSYPNSHGAIAQAAKHLTPELMEGEFRK
                     IGRPDLPVYAYHLKPRFRNLIEGELRQLPISQLTVLEEDLELNF"
     gene            2378..3970
                     /locus_tag="VLL06_03895"
     CDS             2378..3970
                     /locus_tag="VLL06_03895"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS30139.1"
                     /translation="MHRRYGFTKWSGVLCMCAIGLVLHHVAWAQLPNETYDFSISKGI
                     VDFSRGHYDKAADLFEQARQAVPNDPEAIEYLGQTLLRLKKYREAETLFQDLTLSYPT
                     RAQGWLGLAISQSQLGKYHEALASLDQAQKLDPKNPLVFFYQGVVTHELKSFNQSPEL
                     FSRAMALSPDLTPSARYYTGVSYYERGLLDQAQKEFEAAIAAGEPESELARSARAILQ
                     QRTAAPKGPKPWDLNLNVSGQYDTNVVLLPIGIQPPGGSTGISQKDDFRTTFYARGEY
                     RPIQTPVWAAGATYGFYQSLHQNLTAFDIQDHAPSVFVQRQLGIVTARLQYAFDYVRV
                     GEDPFLFTHAIQPIFTIAESNSLFSQIQFRYQNKDFQDDRFAGNSFRDGKNWLAGLTQ
                     YAYFANGTGHLRLGYTYDTDRTGGGHPLVATPGVQTHADWDYKAHRLSIGLGVPELWT
                     LKPSFAFDYYHLDYDNPNSFSLGGATKRRDNVMFITATIGRPLTDWLSIAAEYNYTRD
                     QSNLPVFNYARSIFSITLSSRF"
     gene            4210..5634
                     /locus_tag="VLL06_03900"
     CDS             4210..5634
                     /locus_tag="VLL06_03900"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HSS30140.1"
                     /translation="MFSCQSGGVMMDIQRRLVSLVALTLVFGPSLVMAAEGSKEGIGF
                     YTAVLGKATVTHLGEAQVLPVKLQDEVLFKDVIQTETDSRTRALFQDDSMLTVGENSR
                     VEIDEYIYNPEKNVRLAIVKLMQGQVRALVSKVFKANGSKFEIHTPSAVAAARGTYFT
                     VWVENGQSGIINIGEKGRVDFTSGGMTVAVDPGFFSIAQEGQAPSAPQPHQLAPKGHD
                     QQGAPVKSTEKTTSKPVMVYNQGPNVLPQREKADLIQASSPGGLTRALVAVELTTLRE
                     APLVELPAQVLQALNLDARLLKALTTLTSLSRESLGEGGGKTTAATTGTATTGTLTVG
                     PVSVATTSTSAGTVSVGSVSVAAAAPLAPVTSALVAPLAPVVSTVLAPVTPLVSTVLA
                     PVSPLVTTVLAPVAPIITTVVAPVVPVITTVVAPVVPVITTVVAPVVPVITTVVAPVT
                     PIINTVLATPPAVISGALHLLGGH"
     gene            5858..>7680
                     /locus_tag="VLL06_03905"
     CDS             5858..>7680
                     /locus_tag="VLL06_03905"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSS30141.1"
                     /translation="MKFSLSNTPGFGIPLGIGLGVAITIGLAFLAWLAPNIVVRLDNS
                     ALDVQFRLRGERSPGQEVLLVLVDEKSLKEIGRWPWPRDTQARLIDHIHEGEPAVVGL
                     DIIYAEPEESSTVREIKTWLSAKPQKVPLPDNVLGLLEACLQALDTDRQFAQSIARAG
                     TAVLAMPFLVPEGQTAGPLSDATHVTPEHLRRSEFMLVKQTKSAEESHPYDATAMLPP
                     LDMFAQQARSLGHVYRLPDHDGVTRREVLALRYEDAYYPSFSLEVARLYLGQTREHMA
                     LLLGEGVQLGSTVVPTDQKLRMLINYAGRELRFPWVSATDVIHHRVSSDLFYGKAVLV
                     GTAALGTYDQLSTPFSANFPGVEKNATVIENILHQQFLTAGLWTGPVEFGLVMFFGLA
                     LTFTLPRVRIVPGTVLTAVTWIGYATAVQTLFVVKGVCLPVVMPTVTIGSVFMVTTVL
                     NYVLKERQAREIHSMFANYVSPRIVQELLKSPSKATLGGQRKELTMLFADLVGFTTFS
                     EHRPAEEVVGQLNEYLHAMTDVIFRWNGTLDKFVGDEIVVFWGAPLDQPDHAELALQC
                     ALEMRARLMELQIQWKTSGKPILDNGIGINTGLALVGNIGA"
BASE COUNT         1719 a         2197 c         2086 g         1678 t
ORIGIN      
        1 ggagcttgac tcgcctaccc agacaccatg accccgcttg gccagtgacc gcacacaggc
       61 gaccgcctgg ttctgatttc cgtcgagaac cagaatcctc atggcgtttt gtgcctcgat
      121 cggcatagac aaacggccgg cgctggtccg gagcagtctc ttctcgaata agcctcgcga
      181 catagcggcc ttgctggact gcgacgggag caaggccctc cagaggcttc ccgttcggat
      241 cgacacaatg ggcggaatct ccaatgacaa agatccagga ttcaccagga atcgtcaggt
      301 ctgctcgaac ctggatccga ccggacttgt cctgcggaac cgcgagcgag ttcaaaagcg
      361 gcgacgccct gttgcctgca gcccagatga tattcgtcga tcgaacgaac tggcttccga
      421 tcatcacgcc atcggctcgg acctcactca cctggctgtt cagcctaatt gtgacaccct
      481 tgcgttcgag cgcggtcgcc gctttggccg acaggtcagc actgaaccct gagagaatgc
      541 gatcgccgcc ttccacgagc aggattgcaa gatcattgag ccgcaagttc ggaaaatccg
      601 gtcccatggc cttccgtccg atctccgcca atgcgccggc caactctaca ccggtgggac
      661 ctccccccac gatgacgaag gtgagcccat ccctcgtgcc ggtggcgatt cgtcgtcgtt
      721 ccgcctcctc aaatgccaag agcatccgct cccgaagata gatcgcgtcg gcgagggtct
      781 tgagcccggg ggcggactgc tcccacgaat cgtgcccgaa atacgcgtgc ctcgaccctg
      841 gtgcgacgat gagggcgtcg aacgcaatcg gtgcgctatc ccgcaaccgc acaagacgcg
      901 ccgctcgatc aatggacagg acttcatcca tgaccacgcg aacattgtgg tacgggcgaa
      961 agattgtacg taaaggccag gcgatatctc ctggagagag cgcggcagtg gccacttggt
     1021 agagcaacgg ttgaaacagg tgatgattgg tgcggtcgat gagcaccacg tcgacatcac
     1081 gcagcgattg cgcagccgtc aggcctccga atccgccccc gatgatgacg acctgtttgt
     1141 gaggcatgaa tgggaactaa tgataggcta cgttcctgaa aggaaggtgt caactttctg
     1201 aagggaccga cgccgcacac cgtgacacag ggcccggtat aaatctcaag attctaaagc
     1261 cagaaccgtc attcccgcca tctgttagaa gttgagttcc agatcttctt ccagaaccgt
     1321 cagctgtgaa atagggagct ggcgcagttc tccttcgatc agatttcgga agcgcggttt
     1381 cagatgatag gcatagacgg gaagatcggg acggccgatt tttctgaatt ctccctccat
     1441 aagttccggt gtgagatgtt tcgccgcctg ggcaatggcc ccatgggaat tggggtagga
     1501 gacttcaata aaggccgctt tcaagctcgg catggacgac gcggctcgcc agagatcttc
     1561 tgtttgatac gtgtctccac tgtacaagat ggtggtgtgt tcatcgctga tcaagaatcc
     1621 gactgtcgga acaagatggt tgaccctgac cggtacgatg cgcagaccgg ccgccatcca
     1681 ttcacaattg agccgcaatt tctctgagac cagcacgggc cggtcggcat ccgggagccg
     1741 gaagaagtcc ggatacaccg aggagttgaa gacatgcgca cggaggccat ccaacacttc
     1801 cggaatgctg gcgaccacga gcgagctgcc cgtttcgccg gcgaggttgt cagcgagagt
     1861 cgggagatcc ttgatgtggt cgaagtggag atgcgaaaga aggacgtgac ggatcttcgt
     1921 ttgctccggc aaggtcaacc gggacccgac agtgccggca tccacaagga cagtgtcatt
     1981 gatcaagaag gcacagggcc ggcattgcat ggtccggccg ttaccatcga gaagctgatc
     2041 ggaaccatga cagcccaaca cgcggatttt cacacgaaat ccctttgtcc aaatacaacc
     2101 aagcaggtaa gcacactcgc gacggaccac tcatgtccat tcaagaagca aatcctagag
     2161 ttcttccctg ggcgaactct agcacaaagt cccgatcttg cctcgtagaa ctcggcttcc
     2221 gacgcgcggt ctttttcgag tgaacgaccg gatctgtaga tcatgagtct tgcaacaatt
     2281 cctgcaattc gttgactgat acgttcgctc tgttactctg cctggaagga aaggatgttc
     2341 gtagacgttc tccagcccca gatccaagcc aatcaccatg caccgacgat acggattcac
     2401 caaatggtcc ggcgtgctct gtatgtgcgc tattgggctc gtgctccatc atgtcgcatg
     2461 ggcacagctg ccgaatgaga cctatgattt cagcatctcg aaagggatcg tggattttag
     2521 ccggggacac tatgacaaag ccgccgatct cttcgagcag gcacgacaag ctgtaccgaa
     2581 tgacccggaa gcgatcgaat acctgggaca gacgttgttg cgtctgaaaa agtaccgtga
     2641 agccgaaacg ctctttcagg atctgacact cagttatccg actcgtgcac aaggctggct
     2701 cggactcgcc atctcgcaaa gtcaactcgg aaagtatcac gaggcgctgg catcgcttga
     2761 tcaggcccag aaactcgatc cgaagaatcc gctcgtcttc ttctatcagg gagtcgtgac
     2821 gcatgaactg aaatcattca atcaatcgcc cgaattgttt tcccgggcca tggcgctgag
     2881 cccggatctc acaccgagcg ctcgctacta tacgggggtg tcatattatg agcgtgggct
     2941 gctcgatcag gctcaaaaag aattcgaagc tgccatcgcc gcaggcgaac cggagtccga
     3001 gctggctcga tcggctcggg caattctgca gcaacgaact gccgctccta aaggccccaa
     3061 gccgtgggac ctgaatctga acgtcagcgg ccaatacgat actaatgttg tgttgcttcc
     3121 aatcggcatc caacctcctg gcggatcgac tggtatttcg cagaaggacg actttcgcac
     3181 gacattctat gcacggggtg aatatagacc gattcaaact cctgtgtggg ccgcaggggc
     3241 tacgtacggc ttctatcaga gtcttcatca gaacctgacg gctttcgata ttcaggacca
     3301 tgcgccctcg gtattcgttc aacgtcagct cggcatcgtg acggcccggc tccagtatgc
     3361 cttcgactat gtgcgtgtcg gtgaagatcc atttctgttc acgcatgcca tacaaccgat
     3421 cttcacgatc gccgagagca acagtctgtt ctcccagatt cagttccggt atcagaacaa
     3481 ggactttcag gatgatcgct ttgcaggaaa ttctttcagg gacggaaaaa actggttggc
     3541 gggcctcacg caatacgcct atttcgccaa tgggactgga cacctgcggc taggttatac
     3601 atatgacacc gatcgcaccg ggggcggaca tcctctcgtc gcaacaccgg gtgtacagac
     3661 acatgccgat tgggattaca aagcgcatcg tctgtcaatc ggattgggcg tgccggaact
     3721 ctggacgctg aagccaagct ttgcattcga ctactaccac ctggattacg acaacccgaa
     3781 ttcattttct ctcggcggcg ccacgaagcg tcgcgacaac gtcatgttca tcaccgccac
     3841 gatcggccgg cccctcaccg attggctctc aattgccgcg gaatacaact acacgaggga
     3901 tcagtccaat ctccctgtct tcaactacgc ccgcagtatt ttctcgatca ccctcagcag
     3961 ccgtttctaa gaccttcttc gcccattcag caaagacgca aattgtcgta tgaagcgcca
     4021 aaatttgtcg cgtaacatat tgataataca gcgaaagtgt taacactttc gagggggatc
     4081 atcagaagca gacctcggtg tggttctgtc ttatcaacag cttggagaca gagccgatcc
     4141 tgctcgaaaa aggccggtct cggtacagtt catgcccata ccggaaatgg cgagaagcgc
     4201 gagacgccca tgttttcttg tcaatccgga ggggtcatga tggatattca acgacgactg
     4261 gtcagtctcg ttgcactcac cctggtcttt gggccgtccc ttgttatggc agcagaagga
     4321 agcaaggaag gaatcggatt ctatacggca gtcttgggta aagccacggt cacgcatctt
     4381 ggtgaggctc aagtacttcc ggtcaagctc caagacgagg tgctcttcaa ggatgtgatt
     4441 cagaccgaaa ccgattcgcg cacaagagcg ctgtttcaag atgacagcat gctcaccgtc
     4501 ggagaaaaca gtcgagtgga gatcgacgag tatatctaca acccggaaaa gaatgtccga
     4561 ctggcaattg tgaagctcat gcaaggccaa gtgcgggctt tggtaagcaa ggtgttcaaa
     4621 gcgaacggat cgaaattcga gatccataca ccctcggccg tcgcggcggc gcggggaacc
     4681 tatttcaccg tatgggtcga aaatggccag tccggcatca tcaacattgg tgagaaaggg
     4741 cgcgttgatt ttacgtccgg cggaatgacg gttgccgttg atcctggttt cttctcgatt
     4801 gcccaggaag gtcaggctcc gtcggccccc cagccgcatc aacttgcccc caaaggccat
     4861 gaccagcagg gagcgccggt gaagagcacc gagaagacaa cctcaaaacc cgtgatggtc
     4921 tataatcaag gtccgaatgt tcttcctcaa cgcgagaagg ccgacctgat tcaggcatcc
     4981 tccccaggtg gactgactcg cgcattggtg gcggttgagc tcacgacatt acgagaagcg
     5041 cctctcgttg aattgccggc acaagtcctc caggcgttga accttgatgc gcgcttattg
     5101 aaggcactga cgaccttgac cagcctgtcc cgcgagtctc tcggtgaagg aggcgggaag
     5161 acgactgcgg caaccaccgg aacagctacg accgggacgc tcactgtggg acctgtttct
     5221 gtcgcaacta catcaacttc cgcagggacc gtatctgtag ggtctgtttc tgttgcagct
     5281 gcagctcctc tggccccggt gacctctgct cttgtggccc ctttggcccc cgtagtttca
     5341 actgttctcg ctcccgtcac tcccttagtc tcaactgttc tggccccggt ctctcctctc
     5401 gtcactacag tgctggcccc agttgctcct atcatcacaa cagtcgtggc tccagtcgtt
     5461 ccggtgatca ccactgttgt ggccccagtc gttcccgtca tcaccactgt tgtggcccca
     5521 gtcgttcccg ttatcactac tgttgtggcc ccggtaaccc cgattatcaa taccgtgctc
     5581 gcaactcctc ccgcggtcat tagcggtgcg ttgcatttgc tgggcggtca ctagtggtgg
     5641 attgacgctg ccagggtgaa cgttcttccg ttatcaatat ggtttctgcg gcccttggct
     5701 cgggaagtca agggccgcat ttttttgact ggtgatcgat gtagaacctg gagggtgctg
     5761 actctgcaaa ttatcttgtc cctccgatgt aacaaggcac aatggtaccc agtacgcggc
     5821 acggcactca catgctttga acggtgacaa ctagctcgtg aaattctcac tatccaacac
     5881 cccgggattc ggaatcccac tcggcatcgg cttgggcgtg gctatcacaa tcgggctggc
     5941 ttttctcgca tggctggctc ctaacatcgt cgtgcgcctg gacaattctg cgctggatgt
     6001 ccagttcagg ctgcgaggcg aacgaagtcc tggccaagaa gtcctgctcg tattggttga
     6061 cgaaaagagc ctcaaagaga ttggccgatg gccctggcct cgcgatacac aagcgcggct
     6121 catcgatcac attcacgagg gcgaaccggc agtggtcggc ctcgatatca tctatgccga
     6181 accggaagaa tccagcaccg tccgtgagat caagacatgg ctgtccgcga aaccacagaa
     6241 ggtaccgctg ccagacaacg tgctcggtct gttggaagca tgcctccagg cgttggatac
     6301 ggacagacag tttgcccaga gcatagcccg agccgggacc gccgtgctgg ccatgccatt
     6361 cttggtgccg gagggtcaaa cggccggccc attgtccgac gcaactcatg tgactccaga
     6421 acatctgaga cgaagtgagt tcatgctggt caagcagacg aagtctgcgg aagagtcgca
     6481 cccctatgac gcaacggcga tgcttcctcc gcttgacatg tttgcacagc aggcacggag
     6541 cctcggacat gtctatcgat tgcccgatca tgatggtgtc acccgccgtg aagtactcgc
     6601 cttgcgctac gaggatgcct actatccctc tttttcgctg gaagttgcgc ggctctacct
     6661 gggccagact agagaacaca tggcgctttt gctgggggag ggcgtgcagc ttggttcgac
     6721 ggtcgtcccg acggaccaga agctccgcat gctcattaac tatgcggggc gggagctgcg
     6781 gtttccatgg gtctccgcaa ccgatgtgat ccaccatcga gtgtcatcgg atctctttta
     6841 tgggaaggca gtcctagtcg gaacagctgc attgggaaca tacgatcagc tcagtacacc
     6901 cttctccgcc aattttcctg gagtcgaaaa gaatgccaca gtgatcgaaa acattctcca
     6961 tcaacagttt ctgaccgccg gactttggac cggtcccgtc gaattcggtc tggtgatgtt
     7021 cttcggactc gccctgacct tcacattgcc acgagtgcgg atcgttcctg gaacagttct
     7081 gactgcagtg acttggatcg gttatgcaac ggccgtccaa acgctctttg ttgtcaaagg
     7141 tgtctgccta cccgtcgtga tgccgaccgt gaccatcggg tctgtgttta tggtgactac
     7201 tgtcctcaac tacgttctca aggagcgaca ggcccgagag attcattcca tgtttgccaa
     7261 ttacgtgagc ccccgaatcg tgcaggaact cctgaaatct ccatcaaagg cgacgcttgg
     7321 aggccagcgc aaagaattga ccatgctgtt tgccgacctg gtcggtttca cgaccttcag
     7381 cgaacatcgt ccggctgagg aagtggtcgg tcaactcaac gaatatctcc atgcgatgac
     7441 cgatgtgatt tttcggtgga acgggacgtt ggacaaattc gtcggagacg aaatcgtcgt
     7501 cttctgggga gcgccgttgg atcagccgga ccatgccgaa ctggccctac aatgcgcgct
     7561 cgagatgcgg gcccgactca tggagttaca gatccagtgg aaaaccagtg gaaagcccat
     7621 cctcgataac ggtatcggca ttaacacagg cctagcccta gtgggcaaca tcggcgccga
//
LOCUS       DATIPF010000152         2617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_471135, whole genome shotgun sequence.
ACCESSION   DATIPF010000152 DATIPF010000000
VERSION     DATIPF010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471135"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="VLL06_03910"
     CDS             complement(<1..1461)
                     /locus_tag="VLL06_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSS30142.1"
                     /translation="MATTTETVRLTIDGISISVPKGTLVIEAARQAGVMIPHFCYHPK
                     LKPDANCRMCLVEIERVRKLQTSCSTVATEGMSVRTATTVVNDAHKSVLEFILANHPL
                     DCPVCDQGGKCDLQDFSHQYTATSRFAETKRIFQKEYFSPLIETQMNRCVQCLRCVRY
                     CDEVMDVKALAPIGRGTMTEIKSFGAHPLDCEFCGGCVQICPVGAITSRLSMYEYRPW
                     MLKRADTICTFCGDGCQITVQTKDQELIEVNSAHGGGRNNGDLCARGFFGFHAASHPD
                     RITHPLIKRNGVLVKVTWEEVLEFVAEQTNRLKLAHGPQAFGGLISGRCTNEELYLFQ
                     KFMRLTIGTNNLDSSARYGHVNGVRAMRRVQGTHRWTVSFEDITEADVLLLVGTNITE
                     ANPITGLKVKEAVKKHDAVLLTIEALQPAIGTISNITNLSQQHLCMAPSQFGAAVLGL
                     LKAVVEGDHVDSALRQRASDYVSAVSQAVQQLTWDDL"
     gene            complement(1569..>2617)
                     /gene="nuoF"
                     /locus_tag="VLL06_03915"
     CDS             complement(1569..>2617)
                     /gene="nuoF"
                     /locus_tag="VLL06_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HSS30143.1"
                     /translation="SEPGTFKDRQLIERDPHQLLEGIVLACYAIGAESAYIYIRGEFV
                     LGAKILEQAIAEARIAGYIGTHILGTGITANVWVHRGAGAYICGEETALLESIEGKRG
                     LPRFKPPFPATNGLYNKPSVVNNVETLANIPHILSRGPEWFASIGSPPKSTGTRVFCV
                     SGHVKQPGNYEVPMGVTFRELIYEHAGGMRSDKPLKAFIPGGASAPFLTQDHLDVKLD
                     FESVAAAGSMLGSGGVTVMEEGTPMVWAALRLMEFFYHESCGKCSPCREGSSWLVQTL
                     RRIIAKRGQMEDLETLVDLCKNIEGRTICAFGEAEVAPIMSTLKYWRSEYVTLIQEAE
                     AANLIKLDPIVARH"
BASE COUNT          551 a          749 c          711 g          606 t
ORIGIN      
        1 cagatcatcc cacgtcagtt gctgaaccgc ttgagagacg gccgagacat agtccgatgc
       61 ccgttgacgg agcgcactgt cgacatggtc tccttcaacg acagccttca gcaggcccag
      121 gacggctgcg ccgaactgag acggtgccat acagagatgc tgctgcgaca ggttggtaat
      181 gttgctgatc gtgccgatcg caggctggag cgcctcgatc gtcaacaata ccgcgtcatg
      241 tttcttcacg gcttccttca ccttaagccc cgtaatggga ttcgcttcgg tgatgttggt
      301 gccaactagg agaagcacgt ccgcttccgt gatgtcttca aacgatacgg tccagcgatg
      361 cgtgccttgc acccgtcgca tcgcccgcac tccgttgaca tggccatacc gtgcgctgct
      421 atcgaggtta ttggttccga tcgtgaggcg catgaacttc tggaacagat agagttcttc
      481 gttggtacag cggccggaaa tcagtccgcc gaaggcttgc ggaccatgcg ccagcttgag
      541 ccggttggtc tgttctgcca caaactccaa cacctcttcc cacgtaactt tgaccagcac
      601 cccgttccgt ttgatcaacg gatgggttat tcggtcggga tggctcgccg catgaaatcc
      661 aaaaaaccca cgcgcgcaca ggtcgccgtt gttccggcca ccgccatgag cactgttcac
      721 ttcgatcaat tcctggtcct tggtttggac cgtaatctga catccgtcac cgcaaaacgt
      781 acagatcgtg tcggcgcgtt tgagcatcca gggacggtat tcgtacatcg agagccggct
      841 ggtgatcgcg ccgaccgggc aaatctgcac gcagccgccg cagaattcac agtccaatgg
      901 gtgggcgccg aatgacttga tctcagtcat cgtacctcgt ccgatcgggg cgagggcctt
      961 gacgtccatg acctcgtcgc aataacgaac acaacgcaaa cattgcacgc aacgattcat
     1021 ttgagtttca ataaggggac tgaaatattc cttttggaaa atgcgcttgg tctcggcaaa
     1081 tcgactggtc gcggtgtact gatgggaaaa atcttgaagg tcacacttcc cgccctgatc
     1141 gcagaccggg caatccagcg gatgattggc caggatgaat tcgagcaccg acttgtgggc
     1201 gtcattcacg actgtcgtcg cggtccgaac cgacataccc tcggtcgcca cggtgctgca
     1261 agaggtttgg agttttcgca ctctttcgat ttcgaccaga cacatgcgac agttcgcatc
     1321 cggcttgagt ttcggatggt agcagaaatg tggaatcatc acgccggctt ggcgagcggc
     1381 ttcgatcacc aacgtgcctt tcggcactga gatggagatg ccatcgatcg tcaagcgaac
     1441 cgtttcagtc gtcgtagcca tcgttaggat tgtgccatga gccctgttat tagttaacga
     1501 taactgttaa acgcgcgatg cgagtcattc ctttgcgatt cacgatacat aatttacgtt
     1561 tcacgtcttc aatgccttgc gacgatcgga tccagcttga tgagattcgc tgcctccgct
     1621 tcctgaatca acgtgacgta ctcagaccgc caatatttga gcgtgctcat gatcggcgcc
     1681 acttctgctt cgccgaacgc gcaaatcgtt cggccttcga tgttcttgca tagatcgact
     1741 aaggtttcaa ggtcttccat ctgtcctcgc ttcgcgatga ttcggcgcaa ggtttggacc
     1801 agccaagagc ttccctcacg gcaggggcta cacttgccac aggactcatg atagaaaaac
     1861 tccatgagac ggagcgcggc ccacaccatg ggtgtgccct cttccatcac ggtcaccccg
     1921 cctgagccga gcatggagcc ggctgctgca acagactcaa aatcgagttt gacatcgagg
     1981 tggtcctgag tgaggaatgg cgctgacgcc ccccctggga taaaggcctt gagcggcttg
     2041 tcggagcgca tgcctccggc atgctcatag atcaattcgc ggaaggtgac gcccatcggc
     2101 acttcgtaat tccccggctg cttcacatgg ccgctgacgc aaaagactct ggtaccagtg
     2161 ctcttcggtg gggaaccgat cgaggcgaac cattcgggac cccggctgag gatgtgaggg
     2221 atgttcgcca gggtctcgac gttgttcacc acggacggtt tgttgtagag gccgttcgtc
     2281 gcaggaaaag ggggtttgaa gcgaggcaag ccgcgtttgc cttcaatcga ctccaacaat
     2341 gccgtttctt ctccgcagat ataggccccg gcgccgcggt gcacccacac attggcggta
     2401 attcctgtgc ccaggatgtg tgttccgata taaccggcga tccgtgcttc ggcaatcgcc
     2461 tgttccaaga tcttggcccc cagaacgaac tctcctcgta tataaatgta ggcggactct
     2521 gctccaattg cataacaggc gagcacgata ccttccaaca actggtgggg atctcgttcg
     2581 ataagttggc ggtccttgaa cgtgcctggt tcgcttt
//
LOCUS       DATIPF010000153         3785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1265083, whole genome shotgun sequence.
ACCESSION   DATIPF010000153 DATIPF010000000
VERSION     DATIPF010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265083"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /gene="lnt"
                     /locus_tag="VLL06_03920"
     CDS             <1..773
                     /gene="lnt"
                     /locus_tag="VLL06_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HSS30144.1"
                     /translation="EREPIYQLQLIAMANRAQAPILFGSPAIRFYPDQRPYLLNSAYL
                     LSPDGQLLGRYDKQHLVPFGEYIPFKSSFLSFLDKLVEGIGDFEAGPGPTVLTLTPKP
                     QAATAGIPGTTSRSVRFGAAICYEVIFPNLVRQFAANGAEFLVTVTNDAWFGQSSAAS
                     QHFAMVVFRSVENHLAFARSANTGISGFIDPFGRIVEATPIFTEQAVKGTMQVWRPDT
                     FYSRHGDVFAYGCAIICALLILFGSFRTKGSEPDAVPV"
     gene            791..1940
                     /gene="prfB"
                     /locus_tag="VLL06_03925"
     CDS             join(791..859,861..1940)
                     /gene="prfB"
                     /locus_tag="VLL06_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_155970262.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HSS30145.1"
                     /translation="MLDEVKNRVRAVEQLVSELRGHLDFTRMTAELAHLEQQTSQPDF
                     WSHAQKAAKISRKKSTIERDLQQWRDIDEKTSDLGALLELAEESGDAALAKELTFELD
                     QFESKLTTLRLELLLSGELDPNNAIVAIHPGAGGTESQDWAQMLLRMYVRWAESKKFK
                     VETLDLLPGEEAGVKSVTLSVTGAYAYGYLKAEAGVHRLVRISPFDANKRRHTSFASV
                     FVYPEVDEDIDLVIEDKDLKIDTFRAGGAGGQNVNKVETAIRMTHLPTGIVVQCQNER
                     SQLQNRNGAMKVLKARLYEIELKKKEAEFNASVGEKKDIAWGSQIRSYVFQPYQMVKD
                     HRTGYEVGQVTSIMDGNLDGFIEAYLKMKQRGMTASSYQQPETDEFDS"
     gene            1970..3454
                     /gene="lysS"
                     /locus_tag="VLL06_03930"
     CDS             1970..3454
                     /gene="lysS"
                     /locus_tag="VLL06_03930"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HSS30146.1"
                     /translation="MSDELNEQQQQRIKKLDALREAGVAPYGTRFEVKDHAGRLIILH
                     GEKTKEELEAEKISCTIAGRIVALRRFGKAGFAVLQDGSDRLQVYLKKDHLSEQGYRI
                     AELLDLGDWIGVTGTLFRTKTNEFTVEVAQLTFLSKALRPLPEKWHGLTDVETRYRQR
                     YVDLIANPEVHGIFATRSRIISGIRTFLIARGFLEVETPMMHPIPGGAAAKPFVTHHN
                     ALNVDLYLRIAPELYLKRLIVGGFPRVFEINRNFRNEGISTIHNPEFTMLEFYVSYAD
                     YQDLIILTEELIASLAQEVLGRTVIDYQGKEITLTPPWRRWSYHQAILEVNNLDPAVL
                     ENQDTAMAAAKHLGIAVDQKWPLFNIVNEIFEETVEPNLQQPTFITDYPIEISPLARR
                     KDSNPGLTDRFELYIAGREIANAFSELNDPLDQRERFEQQAAQRAAGDEEAHLVDEDF
                     LRALEYGMPPTAGEGIGIDRLIMLFTNQASIRDVVLYPQLRPEK"
     gene            3457..>3785
                     /locus_tag="VLL06_03935"
     CDS             3457..>3785
                     /locus_tag="VLL06_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSS30147.1"
                     /translation="MALPYEIFVGLRYLRAKRRNRTISLNTVVSIAGITLGVAALIGT
                     VGIMTGFKEDIQAKILGTTAHIIVQDRMKDSMSDYDPVTKQVATVPGVVAATPFVLKQ
                     VLLSTQTA"
BASE COUNT          898 a         1028 c         1061 g          798 t
ORIGIN      
        1 ttgaacgtga gccgatctat cagctgcaac tcattgcaat ggcgaatcga gcccaggcac
       61 caatcttgtt tgggagcccc gccatacgtt tctatccgga tcagcggccc tatctgttga
      121 atagtgcgta cctcctctcc cctgacggcc aattgctggg gcgctacgac aaacaacatc
      181 tggtaccgtt cggagaatat attcctttca aatcctcatt cttatctttc ctggacaaat
      241 tagtcgaagg gatcggcgat ttcgaggcgg gacccggtcc gacggttctg accctgacgc
      301 caaagcctca ggctgcgacc gcagggatac cggggaccac ctctcgatcg gtacgatttg
      361 gagcggccat ctgctatgaa gtgatctttc cgaatctcgt gcggcaattt gccgcgaatg
      421 gggcggagtt cttggtcacc gtgacaaacg atgcctggtt tggtcagtcg tcggcagcct
      481 cgcaacattt tgcgatggtg gtgtttcgtt cggtcgaaaa tcatctggcc tttgctcgct
      541 cagccaacac cggcatttcc gggttcattg atccgttcgg acgtattgtc gaggcgacgc
      601 cgatcttcac agaacaggcc gtcaagggga caatgcaggt ctggcgccca gatacgtttt
      661 acagtcggca cggcgatgtg tttgcttacg gctgtgctat aatctgcgcg cttttgatcc
      721 tgtttggctc cttccgcacc aagggatctg agcccgacgc agtgccggta taaccagaaa
      781 ggatcgcgcc atgttagatg aagtcaagaa tcgcgtgcgg gccgtcgagc agttggtatc
      841 ggaactccgg gggcatcttt gacttcactc gcatgacagc agagctggca catctggaac
      901 agcagacgtc gcagcctgat ttttggagtc acgcgcagaa agccgccaaa atcagcagga
      961 agaagtccac gattgagcgc gatctccagc agtggcgtga catcgatgag aagacaagcg
     1021 atttgggcgc attgctggaa ttggcagaag aaagcggcga cgccgcgctc gcgaaggaat
     1081 tgacgttcga gctggatcaa tttgagtcga agctcactac cctgcggctt gaacttctgc
     1141 tctcgggcga actggacccc aacaatgcaa ttgtggcgat ccatcccggt gccggtggga
     1201 cggaatcgca ggactgggcg cagatgctgc tccgcatgta cgtccggtgg gccgaaagca
     1261 agaaattcaa ggtcgaaacg ttagatctgc tcccaggaga agaagcgggg gtgaagagtg
     1321 tgacactgtc cgtgacaggg gcctatgcct atggctatct caaggccgaa gcaggcgtgc
     1381 accgactggt gcgcatttca cccttcgatg cgaacaagcg gcggcatacc tcttttgcct
     1441 cggtattcgt ctatcctgag gttgatgaag atatcgacct cgtgatcgaa gataaggacc
     1501 tcaaaatcga tacctttcga gcaggcggcg ccggcggcca gaatgtgaat aaagttgaaa
     1561 ctgccatccg tatgacgcat ctgccgaccg ggatcgtggt gcaatgtcag aacgaacggt
     1621 cacagctcca aaatcgcaat ggcgccatga aggtcttgaa ggcgcgcctc tacgaaatcg
     1681 aattgaagaa gaaagaggcg gagttcaatg cgagcgtcgg agagaagaag gatattgcct
     1741 ggggcagcca aattcgatcg tatgtattcc agccctatca aatggtcaaa gatcatcgga
     1801 ccggttacga agtgggccag gtaaccagca ttatggatgg caatctggac gggttcatcg
     1861 aggcctacct caaaatgaaa cagcgaggaa tgaccgcttc gagctatcag cagccggaaa
     1921 cggacgagtt cgacagctaa cgaggataga cgatccgtac gagcgcgcca tgtcggatga
     1981 actgaacgaa cagcagcagc aacgcatcaa gaagctcgac gccctgcgcg aagcgggcgt
     2041 cgcaccctac ggtacacgat tcgaggtgaa ggatcatgca ggccgattga tcatactcca
     2101 tggtgagaaa accaaggaag agctggaagc cgagaaaatc tcttgtacca ttgcaggacg
     2161 catcgtcgca ctccggcgat tcggcaaggc agggtttgcc gtactgcaag atggatccga
     2221 ccggcttcag gtctatctga agaaagatca tctctcagag cagggttatc gtatcgccga
     2281 actgctcgac ttgggcgatt ggatcggtgt gaccgggacc ctctttcgca caaagacgaa
     2341 tgaattcacc gttgaagttg cacagctgac cttcctgagc aaagccctca ggccgctgcc
     2401 ggaaaagtgg catgggttga cggatgtaga gacccgctat cgacagcgct atgtcgactt
     2461 gatcgccaat cctgaggtgc atggcatctt tgccacccgc agtagaatca tctccggcat
     2521 tcgcacgttc ttgatcgcac gaggcttcct tgaagtcgaa acgccgatga tgcatccgat
     2581 ccccggaggg gcggcggcga aaccctttgt gacccaccat aatgcgctca acgtcgatct
     2641 ctatctccgc atcgcgccgg aactctactt gaaacgcttg atcgtgggcg ggtttcctcg
     2701 cgtgtttgaa atcaatcgga acttccggaa cgaaggcatt tcgacgattc acaacccgga
     2761 attcacgatg ctggagttct acgtgtccta tgccgactat caggacttga tcatcctcac
     2821 ggaagaactc atcgcttcat tggcacagga agtcctcggc aggacggtca tcgactatca
     2881 gggcaaggag atcacgctca cgcctccgtg gcgccgctgg tcgtatcatc aagcgattct
     2941 ggaggtcaac aaccttgatc ctgccgtcct cgagaatcag gacacagcga tggcggcggc
     3001 gaaacatctt ggtatcgccg tcgatcagaa atggccgctc ttcaatatcg tgaacgaaat
     3061 cttcgaggaa acagtcgagc ccaatttgca gcagccgacg ttcattaccg actaccccat
     3121 tgaaatttct ccactcgccc ggcgcaaaga ttcgaacccg ggattgaccg atcgcttcga
     3181 actctacatt gccggccggg aaatcgcgaa cgccttttcg gagttgaacg atccgctgga
     3241 tcaacgcgaa cggttcgaac agcaagccgc gcaacgggcg gcaggggacg aagaagccca
     3301 tctggtcgat gaggacttcc tgcgcgcctt ggagtacggc atgccaccaa ccgcgggcga
     3361 aggtatcggc atcgatcggc tgatcatgct ctttaccaac caagcgtcga ttcgcgacgt
     3421 ggtcctctac ccacaactga gacccgagaa gtaacggtgg ctctccccta cgaaatcttc
     3481 gtcggattgc ggtatctgcg cgcgaagcgt cggaatcgca cgatctcatt aaatacagtg
     3541 gtctccattg ccggtatcac actcggtgtg gccgccctta tcgggacagt cggtatcatg
     3601 actgggttta aagaggacat tcaggccaag atccttggaa cgacggctca tatcatcgtg
     3661 caggatcgga tgaaagacag catgagtgat tacgatcccg tcaccaagca agtggcaacc
     3721 gtgccgggtg tcgtggcggc aacccctttt gtactcaagc aggtcctgct ctcgacccag
     3781 accgc
//
LOCUS       DATIPF010000154         3818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1533546, whole genome shotgun sequence.
ACCESSION   DATIPF010000154 DATIPF010000000
VERSION     DATIPF010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533546"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..1987)
                     /gene="ccmI"
                     /locus_tag="VLL06_03940"
     CDS             complement(308..1987)
                     /gene="ccmI"
                     /locus_tag="VLL06_03940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HSS30148.1"
                     /translation="MTVIFWVIASTMTILVLGLLLWPLLQRTTTSATGEEEKTLSIFR
                     QQFAELGQDRANGVLTEELYQQARRELERRLLEETGSTETTPKMAQRQMQGRPVALGL
                     AVIVPIASGLLYWQLGNPLAMTEPPAASLSAQRGSEDGGQFSEGMGPLIERLKQKMEQ
                     NPNDGVGWALLARSYMGMGRYPEAALAYENATTLIPDDAQLLADYADAMGVLHGRKLE
                     GKPEALIQKALKIDPRNVKALMLAGTVAFNRNNFARAAKDWELARANLPAEVDPEMTQ
                     QLVAAIEEAKSHLGGRQDMVSALTEPAAAPARPAAQSGQPRAIRGTVTMAPSLAGKGS
                     PTDTLFVFAREMNGPPMPVSIVRATRKDLPFTFQLDDSTSPMPSRKLSSAGPVVIVAR
                     LSKSGQAMPQNGDLEGMSQPVQSGVDGLTVVIDRERPYAESATSAQPAAQAGQPRMLR
                     GTVTMAPGLAGKGSPTDTLFVFARETSGPPMPVSIVRATRKDLPFTFQLDDSTSPMPS
                     RKLSSAGPVVIVARLSKSGQAMPQSGDLEGTSQPVQSGVDGITVVIDRERP"
     gene            complement(1984..2430)
                     /locus_tag="VLL06_03945"
     CDS             complement(1984..2430)
                     /locus_tag="VLL06_03945"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HSS30149.1"
                     /translation="MVILLLPSLAAAGEAAPLAENPQAEARLKALAVELRCLVCQNQT
                     LADSNAPLAEDLRREVREMIAKNMSDKEIIDFLVTRYGDFVLYRPPLKTTTTLLWVGP
                     FLLLIGGAIALVMALRRRQKKLADVALSEAERNRVAQLLSEGANRL"
     gene            complement(2442..2969)
                     /locus_tag="VLL06_03950"
     CDS             complement(2442..2969)
                     /locus_tag="VLL06_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HSS30150.1"
                     /translation="MNRFLIPLGIFVVVVGFLGVGLTLNPRELPSPLVGRPAPDFSLP
                     QLHDQQKVLSANDLKGKVWLLNFWASWCGGCKTEHPVLIQLAQSGVVPIYGMDYKDKR
                     EDALAWLKEWGNPYPIVAVDESGRAGINYGVYGVPETYVIDKAGVIRYKQIGPLNGDV
                     LQTKILPLIKELQNQ"
     gene            complement(2966..>3818)
                     /locus_tag="VLL06_03955"
     CDS             complement(2966..>3818)
                     /locus_tag="VLL06_03955"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HSS30151.1"
                     /translation="KISVGPPYFDTVFVPLMTPAIFLMGIGPLAQWKQASLPELAMRL
                     RWAFGVSVVTALVLPIVMGQWTPLISLGLLLAIWILTTIAVDLRERLALAGGGGSLFG
                     RLTAMPRAYWGMVLAHCGLALFIVGVTMVKGFEAEQDVRMNVGQTATLGGYTFRFDGT
                     QPYKGPNYIAARATIHVSQDGREVTVMYPEKRRYTVQNQTMTEAAISPGFLRDLYVSL
                     GEPLEDGAWSVRLYHKPFIDWIWGGCLVMATGGVLAISDRRYRLAWRRKEVEVPAGTQ
                     MARQIVS"
BASE COUNT          838 a         1162 c         1007 g          811 t
ORIGIN      
        1 tacgcagaca ccgtcgcggt aagcaccgcg ccgcagacca gcagactaca tctcacccag
       61 tgtttgccaa ccatgtgcaa tactccctgt ttgtgaagac atatcgtatc gtgtgttgcg
      121 tatccctatc ccttcgcgga cagcgcgcat atatctcagc atgcatcaga agatgtcaag
      181 ggggcttgga cactaaatct gatacctgcc tgcaggaaac aaccaccctc aaaagcttac
      241 aggaatcaac ggcaggaact cacacaacat ggtgaagtgg ggggtaggcc agatggactt
      301 cttcgcgcta gggtctttcc cgatcaatga caacggtaat tccatccact ccggattgca
      361 ccggttgact cgtcccctcc aggtctccac tttgcggcat ggcttggccg gacttcgaga
      421 gtcgagccac aatcaccacc ggtcctgcgc tggagagttt tctggatggc attgggctgg
      481 tcgagtcatc gagctggaac gtaaagggta aatcttttct cgtcgcccgt acgatcgaca
      541 ccggcatggg gggaccgctc gtctcgcgtg caaacacaaa caacgtgtcc gtcggagatc
      601 ctttgccagc caggcccggc gccattgtca ccgtacctct gagcatacgt ggctgcccag
      661 cttgcgcagc cggctgagcc gacgttgccg actccgcata tggtctttca cgatcgatga
      721 cgacggtgag tccatcgact ccagattgta ctggctggct catcccttct aaatctccgt
      781 tttgtggcat agcctgacca gacttcgaga gacgagccac aatcacgacc ggtcctgcgc
      841 tggagagttt tctggacggc atcgggctgg tcgagtcatc gagttggaac gtaaagggta
      901 aatcttttct cgtcgcccgc acgatcgaca ccggcatggg gggaccattc atctcgcggg
      961 caaacacaaa caacgtgtcc gtcggagacc ccttgccggc gaggctcggt gccatggtga
     1021 ccgtacctct aattgcacgc ggctgtccgg actgcgcggc cggcctggcc ggcgctgccg
     1081 ccggttccgt caatgcggac accatgtctt gacggccacc aaggtggctc ttggcttctt
     1141 cgattgccgc caccagttgc tgcgtcatct ccggatcgac ctccgcagga aggttcgcac
     1201 gagcgagttc ccaatcctta gccgcgcggg caaaattatt gcggttgaac gccacggtcc
     1261 ctgccaacat caacgccttc acattgcgtg gatcgatctt cagtgccttt tgaattaagg
     1321 cctcaggctt cccttccaat ttgcggccat ggagcacacc catcgcatcg gcataatcgg
     1381 cgagcaactg ggcatcatca ggaatcagtg tggtggcatt ctcgtatgcc aatgccgctt
     1441 cggggtaacg ccccattccc atgtacgaac gcgcgagcaa ggcccatccc acaccatcgt
     1501 tcggattctg ctccattttc tgtttgagcc gctcgatcaa cgggcccatt ccttcagaga
     1561 attggccccc gtcttctgaa cccctttgcg cagacaacga agcggcaggt ggctctgtca
     1621 tagccagtgg gttccccagt tgccaataga gcaacccgct ggcaatcgga acgatgacgg
     1681 caaggccgag agccaccggt ctgccttgca tttgtcgctg tgccatcttc ggcgttgtct
     1741 ccgtgctacc tgtttcttcc aatagacggc gttcaagctc acgtcgcgct tgttgataca
     1801 gttcctccgt gagcacgcca tttgcccgat cctgccctaa ttctgcgaac tgctggcgga
     1861 agatagacag tgtcttctcc tcctctcctg ttgcggaggt cgtcgtgcgc tgcagcagcg
     1921 gccacaacag gaggccgagg acgagaatgg tcatcgtcga tgcaataacc cagaatatga
     1981 cggtcacagg cgattcgctc cttcggaaag cagttgtgcg acacggttgc gctctgcctc
     2041 agacaatgcc acgtcggcaa gcttcttttg tctcctgcgc agcgccatga ccaatgcaat
     2101 cgcacctccg atcaacagga ggaaggggcc tacccaaagt aatgtggtcg tggttttcaa
     2161 cggaggtcga tagagaacga aatctccata tcgcgtgacg aggaaatcga taatctcttt
     2221 gtcgctcata tttttggcga tcatctcgcg aacttctcga cgcaagtctt ccgccaacgg
     2281 cgcgtttgaa tcggccagcg tctggttctg acagactagg caccgaagct cgacagccag
     2341 cgctttcagg cgcgcttcag cttgtggatt ctcagcaagc ggggcagcct ctcccgctgc
     2401 agcgagactg gggaggagca ggatgaccat gaagagccat ctcattggtt ctgcagctcc
     2461 ttgatgagag gcaaaatttt cgtttgcaag acatccccgt tcaacgggcc aatctgcttg
     2521 tagcgaatga cgcccgcctt atcgatcacg taggtttccg gcacgccata gacaccataa
     2581 ttaatgccgg cgcgccccga ctcatccacc gcgacgatag gatagggatt gccccactct
     2641 ttcaaccagg ccagcgcgtc ttccctttta tccttataat ccatgccgta gataggcacg
     2701 acccccgatt gtgcaagctg gataaggaca ggatgctctg ttttgcatcc accgcaccac
     2761 gaagcccaga aattgagcag ccagaccttg cccttcagat cgtttgcgga aagtactttt
     2821 tgctggtcat ggagctgcgg gagcgaaaag tcaggtgccg gcctgccgac taagggagat
     2881 ggcaattcac gcggattgag cgtcagtccg acaccaagaa aaccgaccac cacgacgaag
     2941 atcccgagcg ggatgagaaa ccggttcatg agactatctg ccgagccatc tgtgtccctg
     3001 ccggcacctc cacttctttc cggcgccacg cgagtcggta ccggcgatca ctgatggcca
     3061 acacgcctcc cgttgccatg actaaacaac cgccccagat ccaatcgata aagggcttgt
     3121 ggtagagccg gacactccaa gctccatctt caagtggctc acccaacgac acatagaggt
     3181 cacgcagaaa gccgggagaa atcgcggctt ctgtcatggt ctgattctga acggtgtaac
     3241 gccgcttttc cggatacatc accgtcacct cacggccatc ctgactcaca tgaatcgtcg
     3301 cacgcgctgc aatgtagttc ggtcctttat acggctgggt tccgtcaaac cggaatgtat
     3361 agccgccaag cgtcgccgtt tgcccaacgt tcatcctgac atcctgctcg gcttcgaagc
     3421 ccttcaccat ggtcacacca acgatgaaca gggcaagtcc gcaatgggcc aagaccatac
     3481 cccaatacgc tcgtggcatc gcagtcagtc gaccaaagag gctgcctcca ccaccggcaa
     3541 gggcaagccg ttcgcgcagg tcaaccgcaa tcgttgtcag aatccagata gcgagcaaga
     3601 gaccaaggct gatcagcggc gtccactggc ccatcacaat cggcagtaca agggctgtca
     3661 caacactgac gccgaatgcc cagcgcaggc gcatcgcgag ttccggcagg ctggcttgct
     3721 tccattgagc cagagggccg atccccatca agaaaatggc aggcgtcatc agtggcacaa
     3781 agacggtatc aaaatagggt gggccgacag agattttc
//
LOCUS       DATIPF010000155         6803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_383191, whole genome shotgun sequence.
ACCESSION   DATIPF010000155 DATIPF010000000
VERSION     DATIPF010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383191"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /gene="tuf"
                     /locus_tag="VLL06_03960"
     CDS             <1..141
                     /gene="tuf"
                     /locus_tag="VLL06_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031793.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HSS30152.1"
                     /translation="EMVMPGDNVSVTGELISPIAMDQGLRFAVREGGKTVGSGVVTEI
                     LA"
     gene            168..482
                     /gene="rpsJ"
                     /locus_tag="VLL06_03965"
     CDS             168..482
                     /gene="rpsJ"
                     /locus_tag="VLL06_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HSS30153.1"
                     /translation="MVKVEQRIRIRLRGFDYRVLDQSVAEIVETVRRSGAKIVGPIPL
                     PTRIEKFTVQSATHADKKAREQFEIRTHKRLMDIMDATPETMDSLMKLNLAAGVDVEI
                     KL"
     gene            512..1132
                     /gene="rplC"
                     /locus_tag="VLL06_03970"
     CDS             512..1132
                     /gene="rplC"
                     /locus_tag="VLL06_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HSS30154.1"
                     /translation="MTNGLLGKKLGMTQIFDESRFTPVTVIEAGPCRVVTIKTKERDG
                     YESVQLSFGEVKERKLSKAELGHLKKTEAPATRVLREFQKVGDVAVGQSIKVDIFKKG
                     DWVDVIGISKGKGYQGVVKRHHYAGGPESHGSMFHRHPGSMGASSYPSRVWKGKTLPG
                     HMGAKRITAQRLKVIESRPDENLLFVRGAVPGAANGLIMVRKSKKG"
     gene            1136..1759
                     /gene="rplD"
                     /locus_tag="VLL06_03975"
     CDS             1136..1759
                     /gene="rplD"
                     /locus_tag="VLL06_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HSS30155.1"
                     /translation="MPTVDVVDLKKQKVGSVDLPDEVFGCKLHSALVHEAVVMQRACE
                     RQGTASTLRRGEVAGSGKKPWKQKHTGRARAGSIRSPVWRHGGSVFGPKPRDYSYSMP
                     KKKYRAALQCALSAKLADGQVVIVSNLSLDQPKTKLLARALTHLGAGAYALIVAGAGH
                     AGLAQAAGNLPNVCVVGPEGLNVYDIVRAELILILERELPRVKEVWA"
     gene            1756..2049
                     /locus_tag="VLL06_03980"
     CDS             1756..2049
                     /locus_tag="VLL06_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HSS30156.1"
                     /translation="MKAGLHSILLQPLLTEKITAMRESNNTVGFLVHPDANRVQIKQA
                     VESLLKVKVERVNVMNVRGKVKRLGRFSGKRSDWKKAFVKLKQGEKLELYESA"
     gene            2100..2924
                     /gene="rplB"
                     /locus_tag="VLL06_03985"
     CDS             2100..2924
                     /gene="rplB"
                     /locus_tag="VLL06_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HSS30157.1"
                     /translation="MGIRAYKPRTPGRRGMTAVTTEELSKKRPEKSLTSFRRSTGARN
                     NEGRTTVRFRGGGHKRLYRKIDFRRDKAGIPGTIAALEYDPNRSARIALVHYKDGEKR
                     YILAPVGLKVGDVVQSGVGSEVRLGNALPLVSMPLGTTIHNIELKPGKGGQLIRSAGG
                     AAQVMGRDGEYVQVRLRSGEMRRILSTCMATVGQVGNTDHENINVGKAGRTRWLGRRP
                     NVRGVVMNPVDHPHGGGEGKSGQGNPHPVSPWGLPTKGYKTRNNKSTDKFIISRRK"
     gene            2934..3221
                     /gene="rpsS"
                     /locus_tag="VLL06_03990"
     CDS             2934..3221
                     /gene="rpsS"
                     /locus_tag="VLL06_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HSS30158.1"
                     /translation="MPRSVSKGAFVDDHLLQKVERMNQNKDRKIIKTWSRRSTVIPDM
                     IGHTFAVHNGKKFIPVFVTENMVGHKLGEFAPTRFFKGHGHARTEKAVALK"
     gene            3265..3618
                     /gene="rplV"
                     /locus_tag="VLL06_03995"
     CDS             3265..3618
                     /gene="rplV"
                     /locus_tag="VLL06_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HSS30159.1"
                     /translation="MAEARAVLRFVRVSPRKARPVIDLIRGQQVPMALALLRNLPRQA
                     SKVIEKILRSAVANAEQKEMGDSESMVVSKAFVDCGPTLKRFRSRSQGRANAIHKRMS
                     HITVVVSAASMKEKK"
     gene            3674..4345
                     /gene="rpsC"
                     /locus_tag="VLL06_04000"
     CDS             3674..4345
                     /gene="rpsC"
                     /locus_tag="VLL06_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HSS30160.1"
                     /translation="MGHKTHPIGYRLGYNVTWSSRWYASKDYAKLLHQDIKIRKMVKQ
                     RLFHAGVAKVEIERSGDQTRVIIHTARPGIIIGRKGAEVDKLKAELEKSYSGQVYITV
                     KEIKKPELDAQLVCENVATQLEKRVAFRRAMKRSVQSALRLGAQGIKIMVGGRLGGAE
                     IARSEWYREGRVPLHTLRADIDYGFAEAHTTMGQIGIKTWIYKGEVLPLPPLNKESVF
                     DRRLG"
     gene            4360..4773
                     /gene="rplP"
                     /locus_tag="VLL06_04005"
     CDS             4360..4773
                     /gene="rplP"
                     /locus_tag="VLL06_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HSS30161.1"
                     /translation="MLAPKKVKFRKMMKGRMTGKAYRGSQITLGEFGLKALEPGWITS
                     RQIEAARIAITRCVKRGGQVWTRIFPDKPITKKPAETRMGKGKGNPEYWVAVVKPGRI
                     LYEMDGVTPDVAKEAFRLASHKLPIATKCVIRGEF"
     gene            4814..5035
                     /gene="rpmC"
                     /locus_tag="VLL06_04010"
     CDS             4814..5035
                     /gene="rpmC"
                     /locus_tag="VLL06_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HSS30162.1"
                     /translation="MDLKELRQLTGPELAEKMQQFTQELFNFRFQLGTGRLENPMQVR
                     KTKRSIARIKTIQRELAQVEPHSTAAKGA"
     gene            5038..5304
                     /gene="rpsQ"
                     /locus_tag="VLL06_04015"
     CDS             5038..5304
                     /gene="rpsQ"
                     /locus_tag="VLL06_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004154489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HSS30163.1"
                     /translation="MGESVNKRREWVGRVVSNKMNKTVVVEIERSVIHPLYRKVLRRV
                     TTFKAHDEDNACKIGDRVRMVETRPISKDKHMRVVEVVEKGRAE"
     gene            5343..5711
                     /gene="rplN"
                     /locus_tag="VLL06_04020"
     CDS             5343..5711
                     /gene="rplN"
                     /locus_tag="VLL06_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HSS30164.1"
                     /translation="MIQSYTYMDVADNSGAKQAMCFHVLGGTRRRYASLGDIVVVAVK
                     EAIPAATVKKGDVSRAVVVRTTKEVRREDGSYIKFDRNACVLINKDGDPVGTRIFGPI
                     ARELRWKKFMKIISLAPEVL"
     gene            5731..6066
                     /gene="rplX"
                     /locus_tag="VLL06_04025"
     CDS             5731..6066
                     /gene="rplX"
                     /locus_tag="VLL06_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HSS30165.1"
                     /translation="MNKQALKKNRIRKGDVVVVIAGRDRGKSGKVLSVDPGVGKVVVE
                     KLNMIKRHTKPNQKVKQGGILEREAPFAISNVMYLCPVTQKPTRLGVRMLDDGRRVRF
                     SKKSNDTVE"
     gene            6208..6738
                     /gene="rplE"
                     /locus_tag="VLL06_04030"
     CDS             6208..6738
                     /gene="rplE"
                     /locus_tag="VLL06_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HSS30166.1"
                     /translation="MREAYLQKVVPALMKEFGYKNIMQVPKLERIVLNVGMGEAIQNV
                     KLLESAAGELGVITGQKPLITKAKKAIATFKLRQGMPIGTKVTLRSRRMWEFFDRLVT
                     LSLPRIRDFRGVSPKSFDGRGNYTLGLKEQLIFPEIKYDEVASIHGMDITIVTTARTN
                     DEGRALLKHLGMPFRT"
BASE COUNT         1696 a         1510 c         2190 g         1407 t
ORIGIN      
        1 gagatggtga tgccggggga caatgtgagt gtgacggggg agttgatcag cccgatcgcg
       61 atggatcagg ggttgcggtt cgcggtgcgc gagggcggca agaccgtcgg ctccggcgtc
      121 gtcaccgaaa ttctggcgta atgatcggcc gatgaataga ggaatgaatg gttaaggttg
      181 aacaaagaat caggattcgc ttgcgcgggt ttgattaccg cgtgttggac caatcggtgg
      241 cggaaatcgt tgagaccgtc cgccggagcg gggctaaaat tgtagggccg attccgcttc
      301 ctacgcgcat cgagaagttt acggtgcaga gcgcgacgca tgcagacaag aaggctcgtg
      361 agcagttcga aatcagaacg cacaagcgct tgatggatat catggacgcc actcctgaaa
      421 ccatggattc cctgatgaaa ttgaacttgg ccgccggcgt ggatgtcgag attaagctct
      481 aagggtagac gacagacaag gaactgtacg catgacgaac gggttgttgg gtaaaaagct
      541 ggggatgact cagatatttg acgagtcgcg atttacacct gtgaccgtca tcgaggcggg
      601 tccttgccgc gtggttacga tcaagacaaa agaacgcgat gggtatgaat ccgttcagtt
      661 gtcgttcggc gaggtgaaag aacgcaagtt gtccaaggcc gagctgggac atctgaagaa
      721 gacggaggct ccagctaccc gtgtcctcag agagtttcaa aaggttggcg atgtggcggt
      781 agggcaatcc atcaaggttg atatctttaa aaagggcgat tgggtggatg ttatcggtat
      841 ttctaaggga aagggatatc agggagtcgt gaagcggcat cattatgccg gcggtcctga
      901 atcccacggg tccatgttcc atcgtcatcc cggctcgatg ggcgccagtt catatccttc
      961 tcgtgtctgg aaagggaaga ccttgcccgg acatatgggg gccaagcgta ttacagcgca
     1021 acgattgaaa gtgattgagt ccaggcccga tgagaacttg ctcttcgttc gaggggccgt
     1081 acccggcgca gcaaatggac tcatcatggt aagaaagtcg aagaaggggt agggcatgcc
     1141 cacggttgat gtcgttgatt tgaagaagca gaaagtcggg tccgtcgatc ttcctgacga
     1201 agtattcggc tgtaagctgc actcggcctt agtccatgaa gcggtggtga tgcagcgtgc
     1261 ttgtgagcgt cagggaacgg catccacatt gcggcgagga gaagtcgctg ggtccgggaa
     1321 aaagccgtgg aagcaaaagc atactgggcg ggcgcgggct gggtcgatcc gctctcccgt
     1381 atggcgtcat ggcggatcgg tgttcgggcc gaaaccgcgt gattactcct acagcatgcc
     1441 gaaaaaaaag tatcgtgctg cgcttcaatg cgcgctgtcg gcaaagttgg cggacggaca
     1501 agtggtgatt gtgtcgaatc tatcgttgga ccagccaaag acgaaattgt tggcccgagc
     1561 attgacccac ttgggtgccg gggcctatgc cttgatcgtc gcaggtgccg gacatgccgg
     1621 attggctcaa gctgcaggca atctccccaa tgtgtgtgtg gtagggcctg agggattgaa
     1681 cgtctacgat atcgttcggg ctgaattgat tctgattctt gagcgcgagt tgccgcgggt
     1741 gaaggaggtc tgggcatgaa ggcgggtctc catagcattc tgttgcagcc gttgcttact
     1801 gaaaagatca cggcgatgcg ggaaagcaac aatactgtgg gcttcctcgt tcacccggat
     1861 gcgaaccgtg tccagattaa gcaagcggtt gaatcgctgt tgaaggtgaa ggtcgagcga
     1921 gtgaatgtga tgaatgttcg cgggaaggtg aagcgcttgg ggcgtttttc cggtaagcgg
     1981 tcggattgga aaaaagcctt cgtcaagctc aagcaaggcg agaagttaga actctacgag
     2041 agcgcatagc gggatgtagc accgggctga cctcggtgtg ctggaaggac gactgtcgaa
     2101 tgggaatcag agcatacaag ccaaggacac cgggccgtcg cgggatgact gcggtgacga
     2161 cggaagaact gtcgaagaag aggccggaga aatcgctcac gtcatttcgc cggagtactg
     2221 gagccaggaa caacgaaggg cgaacgacgg tacgttttcg tggaggcggg cataaacgac
     2281 tttatagaaa aattgatttt cggcgcgaca aagccggaat tccggggacc attgccgcgc
     2341 ttgaatacga ccccaatcgg tcggctcgga ttgccttggt gcactataaa gatggcgaga
     2401 agcgctatat cctagcccca gttggcttga aagtcgggga tgtggtgcag tcgggtgttg
     2461 gctcagaggt tcgtcttggg aatgcgttgc ccctggtcag tatgccattg ggaacgacca
     2521 ttcataatat agagctgaaa cccggaaaag gcgggcagct gattcgcagt gcgggaggcg
     2581 ccgctcaggt gatggggcgc gacggcgaat atgtgcaggt gcgactcagg tcaggtgaga
     2641 tgcgaagaat cctttcaacc tgcatggcaa ccgtcggcca agttgggaat acggatcatg
     2701 agaatattaa tgttgggaag gcaggtcgga cgcgatggct gggccgacgc ccgaatgtgc
     2761 gcggtgttgt gatgaatccg gtcgaccacc cgcatggtgg cggcgaagga aagtctggac
     2821 agggtaaccc ccatccggtc tccccatggg gccttccaac caagggctac aagacccgga
     2881 acaacaagtc gacggacaag ttcattattt cccgccgcaa gtaggagaac gcgatgccga
     2941 ggtcagtcag taaaggtgca ttcgttgacg atcatctgct tcagaaagtg gagcggatga
     3001 atcagaacaa ggaccggaag atcattaaga cctggtcgcg acggtccacc gtgattccgg
     3061 atatgatcgg acatacgttc gcggttcata acggcaagaa gttcattccg gtcttcgtga
     3121 cagaaaatat ggttgggcac aagctgggag agtttgctcc gaccaggttc tttaaggggc
     3181 atgggcacgc caggacagag aaggcggtcg ctcttaagta gggataggct acggtctcgg
     3241 tacactcgat tggataggac ggttatggct gaagcacgag cagttctgcg gttcgtacgt
     3301 gtctctccga ggaaagcccg cccggtgatc gatttgatcc gtgggcagca agtgccgatg
     3361 gccttggcat tgctcagaaa tttgccgcgt caggcgtcaa aggtgatcga aaagattttg
     3421 cggtccgccg tggcaaatgc agagcaaaaa gagatgggcg acagtgaatc gatggtggtg
     3481 tcgaaggcgt ttgtggattg cgggccgacg ttgaaacgtt ttcgatctcg atcgcagggg
     3541 cgggcgaatg cgattcacaa gcggatgagt catattacag tcgtcgtatc ggctgcaagc
     3601 atgaaagaaa agaagtagat agaagcggca atagaacgga cgcatttcct gtttgagtga
     3661 ggcataagaa gttatggggc acaagacaca tccgatcgga taccggctag gatacaacgt
     3721 cacgtggtca tctcggtggt acgccagcaa ggattatgca aagctgctcc atcaggacat
     3781 taagatccgc aagatggtca aacagcgtct gtttcatgcg ggtgtggcca aggttgaaat
     3841 cgagagatcc ggagatcaga cgcgtgtgat tattcacacc gcgcgccccg gtatcatcat
     3901 cggtcgtaag ggagctgagg tcgataagct caaggcggag ttagaaaaga gctactccgg
     3961 tcaggtctac attacggtga aggaaatcaa gaagccggaa ctggatgccc agttggtgtg
     4021 tgaaaacgtc gcgactcagt tggaaaaacg agtggcattc cgccgagcca tgaagcgaag
     4081 cgtgcagtcc gcacttcgtc ttggagccca aggcatcaag attatggtcg ggggccgtct
     4141 tggcggggcg gagatcgcgc gttcagagtg gtatcgcgaa ggccgtgtgc cgctccatac
     4201 gctccgggca gacatcgact atgggtttgc cgaagcccat acgacgatgg gccagattgg
     4261 gatcaagaca tggatctata agggggaagt tcttccgttg ccgcccctta ataaagaatc
     4321 agtatttgac cgcagacttg ggtaattgaa gagggtatcg tgttagcacc aaagaaagtt
     4381 aagttccgta aaatgatgaa ggggcgtatg accgggaagg cctaccgggg cagccagatt
     4441 accctcggag agtttgggct taaagcgctt gagcctggct ggatcaccag ccgacagatt
     4501 gaggcggcac gcatcgcgat cactcggtgt gtgaagcgcg gtggccaggt atggacgaga
     4561 atattccccg ataaaccgat taccaagaag ccggctgaga ctcgaatggg taaagggaag
     4621 ggcaacccgg agtactgggt cgctgtcgtc aagcccggcc gtattctcta tgagatggat
     4681 ggtgtgacgc cggatgtggc caaggaagcc ttccgtttgg cttcgcataa gttacccatc
     4741 gccacgaagt gtgtcattcg cggcgagttt tagctttgat cgaacagaac gcagaagtaa
     4801 ggggattgta ttcatggacc tgaaggaatt acggcaactc acggggcctg agctggcgga
     4861 gaagatgcag cagttcacgc aggaactctt caactttcgc tttcaactgg ggaccgggcg
     4921 gttggaaaac cccatgcaag ttcgaaagac gaagcggtcc atcgccagga tcaaaacgat
     4981 tcaacgtgag ctcgcacagg ttgaaccaca ttctaccgcg gcgaaagggg cttgaggatg
     5041 ggagaatcag tcaataagcg ccgtgaatgg gttgggcgtg tggtcagcaa taagatgaat
     5101 aagaccgtgg tcgttgaaat cgagcgttcg gtgattcacc cgctctaccg gaaagtgctt
     5161 cgacgagtga caacattcaa ggcgcacgat gaggataatg cctgcaaaat aggcgaccgc
     5221 gtgcgcatgg tcgagaccag gcccatcagt aaagataagc atatgcgggt tgtcgaagtc
     5281 gtggaaaagg gacgggctga gtagatcgaa ggcgtgcgaa gggtagagga aacaccggga
     5341 ttatgattca aagctataca tacatggacg tggccgataa ctccggtgcc aagcaggcca
     5401 tgtgctttca tgtgcttggt gggaccaggc gtcgctatgc ctcgctcgga gacattgtcg
     5461 ttgtggctgt caaagaggcc attcccgcgg caacagtcaa gaagggcgat gtaagccgcg
     5521 ctgtggtcgt gaggaccacc aaggaggtac gtcgtgagga tggttcctac atcaagtttg
     5581 atcggaatgc ctgcgtgcta atcaataagg atggggatcc cgtcgggact cgtatctttg
     5641 ggccgatcgc gcgtgagctc cggtggaaga agtttatgaa gattatttcc ctcgcgccgg
     5701 aagtgctgta gcgagggtgg agacggcggg atgaataagc aggcgttgaa aaaaaatcga
     5761 attcgtaagg gcgatgtggt ggtcgtgatt gccgggcgtg accgtggcaa gtccggaaaa
     5821 gtgctgtcgg ttgatcccgg agtggggaag gttgtggttg aaaagttgaa catgatcaag
     5881 cgtcacacca agccgaatca gaaagtcaaa caaggtggca ttctcgaacg agaagcgcca
     5941 tttgcgattt caaatgtgat gtatttgtgt cctgtgacgc agaagccgac tcggttgggc
     6001 gtgcggatgc ttgatgatgg ccgacgagtc cggttcagca aaaaatcaaa cgacactgtc
     6061 gagtaggagc gaggacgcca gatggcgaaa gttgaatcag gaaaaggtag taagacgtcg
     6121 gagcggcggc cgcccaagaa aaaagaaggg gcgtctgcgc cgcaggcgaa ggaccaaggc
     6181 agcgaagagt ccaagttcaa tccacggatg cgagaggcct acttgcagaa ggttgtgccc
     6241 gcgctcatga aagagttcgg ttacaaaaac atcatgcagg ttccgaagct tgaacggatt
     6301 gtgctgaatg tggggatggg agaagccatt cagaacgtga agctattgga gagtgcagcc
     6361 ggggaattgg gtgtcattac agggcagaaa ccgctgatca ccaaggcgaa gaaggccatc
     6421 gccaccttta agctacgtca agggatgccg atcggcacaa aggtcacgtt gcggagtcgc
     6481 agaatgtggg aattcttcga tcgccttgtg acattgtcat tgccacggat tcgagacttc
     6541 cgcggtgtct cgccgaagtc gttcgatggc cgaggcaatt acacgcttgg actcaaagaa
     6601 cagctcattt tccctgagat taagtacgac gaggttgcat ccatccatgg gatggatatc
     6661 acgatcgtca cgactgccag gaccaacgat gagggccggg cgttgttgaa gcatttagga
     6721 atgccattcc ggacgtaata tcacgcgaag gagctgcagg tgtcgagatt agcgctcaga
     6781 aataaatcgg ccgtcaaggc gaa
//
LOCUS       DATIPF010000156        12972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_29945, whole genome shotgun sequence.
ACCESSION   DATIPF010000156 DATIPF010000000
VERSION     DATIPF010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 12972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12972
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29945"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VLL06_04035"
     CDS             complement(<1..626)
                     /locus_tag="VLL06_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HSS30167.1"
                     /translation="MATSVKTSKHELLRELLRHVSRLFYTTLVVVPADVRDQVSLAYL
                     FARAADTIADTELIDRPRRLDFLGQLKGQFVSDQISWGQIREIQRAMGPLQRDSSERV
                     LLERLEDCFKLFQEFSPDDRRCVQRLMTTLTQGMEMDLSVFPGTSVKDLVALKTIDDL
                     DRYTYYVAGCVGEFWTDLMCAHRRALASWNVREMSDVGVRFGKGLQLT"
     gene            complement(629..1054)
                     /locus_tag="VLL06_04040"
     CDS             complement(629..1054)
                     /locus_tag="VLL06_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HSS30168.1"
                     /translation="MRLSYLLATVGLIISISVTIVDAAEPSQSGPPMVIPVAEDGVQR
                     ATITLDSYSYAPNHLIVEAGKPVELALISVTTIIPHNFIIKSPTGNLSVEQDVSAGKT
                     VTIRFTPIQPGTFPIYCDKRLWPLPSHRDKGMEGTLEVR"
     gene            1278..2165
                     /gene="htpX"
                     /locus_tag="VLL06_04045"
     CDS             1278..2165
                     /gene="htpX"
                     /locus_tag="VLL06_04045"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HSS30169.1"
                     /translation="MKWMKGMFLLLAANILIMVTLSLTVPILINVVLPIFGVDLRGMV
                     DLSSLVWALAFGFGGAFISLAFSKQMARAMLDCRQITQPRSQAEQVIYGSVQEIAQRL
                     LITMPEVWIYDSPDPNAFATGPSKNNSMVAVSTGLLSNLQEQEVRAVLAHEMGHVYNG
                     DMFATTVLAGLMNTFVYYIAMWVRRFFGERDQAMLGFGLSIVIQIIVSVMASVLICWF
                     SRQREFGADAFSAKVYGKNSMISALRAIDRWVTRSQVEYSTQDALATMKISGNSSGFM
                     RLFATHPPMEARIEALERL"
     gene            2303..2800
                     /locus_tag="VLL06_04050"
     CDS             2303..2800
                     /locus_tag="VLL06_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HSS30170.1"
                     /translation="MAAQTPMVYDFTLNDIEGKPVSLSQFRGKVLLLVNTASFCGNTP
                     QYTDLEKMYEQYREKGFEILAFPANNFGQQETGTDAEIKTFCYTKCSLSFPLFSKISV
                     KGDDKHPLYRYLTEQSQFPGEVEWNFQKYLVDRSGKVVARYHHRTKPLTPEVVQDVER
                     VLAAP"
     gene            complement(2806..3090)
                     /locus_tag="VLL06_04055"
     CDS             complement(2806..3090)
                     /locus_tag="VLL06_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30171.1"
                     /translation="METRIKVTDQEKLTLLYERFRDVCLVEKEVWKEIFMPREVTQGP
                     VRTNIQDRYDVEIDDPEIEDALDANIPRGSQSLAAAIQEYRVHISFYRKT"
     gene            complement(3208..3858)
                     /locus_tag="VLL06_04060"
     CDS             complement(3208..3858)
                     /locus_tag="VLL06_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30172.1"
                     /translation="MMIQMIETRPLRKLLLASLLAALSTVACVWQEVEARDLFAPKEQ
                     VTTEVDPTQSPSFDEQKKGIPQSLFTWIKMAKGYDIEWDTFGRKGWFTQDPRLKPIEP
                     GSVFPRDIPAIYIVFESAPLEDPTQFSAQWFLEGEKGVVSSMPLGKDTLEVPGHERYG
                     FLELKKSGDTWEVGTYLVKLFITPLGQQSFHAGNQVGTMRFTIVADTAPVSVNPHK"
     gene            4033..5400
                     /locus_tag="VLL06_04065"
     CDS             4033..5400
                     /locus_tag="VLL06_04065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSS30173.1"
                     /translation="MPTDVKPQSAGERGRLYGLRDGACQAVTQGSGEQYLSAFALLLH
                     ASPFQLSVLSALPQLIGMGAQLVSVKLLQWFPHRKVLISAGTIGQAFTWIPIVLLPLL
                     LPQWGPWLLVGGAAAYFACAHFTMPAWNSLIADWLDQHERGAYFARRAQIIAILSFFA
                     LCGAGWVLSLWQDSTMAWWGFVVIFALAGSARILSALALSPVQDVHQTAHQEAQLGFR
                     GFLRQNMTKDFRRFLLFSGLMHASVLIAGPFFVLYILQDIHLSYFGYGGWLAAGLLGQ
                     LLTLQAWGQFGDRFGNKALLSITGFTVTILPMLYLVSTNVGFLLAVNFLGGVIWAGLA
                     LGLQNYVFDSVRPEDRAKAVALYSTVNAIGWSVGALLGSWLVEHIPARIEWAGIILQP
                     ASNLPFVFFLSGVLRLLVSSSLLGTFHEARQVERIPRQQLLWELPLVKPVAQFVVRTL
                     PKSNP"
     gene            complement(5410..5631)
                     /locus_tag="VLL06_04070"
     CDS             complement(5410..5631)
                     /locus_tag="VLL06_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30174.1"
                     /translation="MEHREREIPRGQKIVGCVMTGILVMAMTACSSDQPKPLAQPTPD
                     QVRGHADRAFDKLKQEEQQRPAQPPVPGY"
     gene            complement(5672..6316)
                     /locus_tag="VLL06_04075"
     CDS             complement(5672..6316)
                     /locus_tag="VLL06_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30175.1"
                     /translation="MNWPRVISRGGMVFLMALSLASCQSSSLPAASPLVVRTIGQSLT
                     IPVDVQRIAIFYPRSSNSDFLEAYQRLEGATFQLKILRPTLKVVDRFHLLAVITEQRL
                     QNAGAVADDSAVRVGHLLGVDSVLLYSIDGPTLRDRLFAHVPSQVRPITVTTKIIRVE
                     SAEIVYFNVVTARMDDGERWDWSPSDNWDYQQQSRAALERGIIQTVSDLQRAFE"
     gene            6500..6946
                     /locus_tag="VLL06_04080"
     CDS             6500..6946
                     /locus_tag="VLL06_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HSS30176.1"
                     /translation="MSLHDRLTEDLKRAMKARDQLRMDVIRMIKAAVMNKEMEIKKDL
                     DDAEMSRVMTTMIKQRRESVEQFEKGNRAELAAKERQEITILESYLPQALSPEQLSTV
                     VDAVIQETGARSLKEMGAVMKAVMVRVVGQPVDGKQISDLVRAKLQ"
     gene            7018..7656
                     /locus_tag="VLL06_04085"
     CDS             7018..7656
                     /locus_tag="VLL06_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS30177.1"
                     /translation="MGILIIDDSPDQQVLLRAILGKAGHTDLLSANSAKTAFPMLDMD
                     GHEPPNQVDLILMDVLMPELDGVEACRQIKSRPHLCDIPIIMVTAKSDLSNLQAAFAA
                     GANDYITKPVNSVELLARASSALALKKEMDCRKAREGELRRSNDALQQALRDVKVLRG
                     LIPICASCKKIRNDGGFWQQLEEYIGEHSEAEFSHGICQPCVKKLYPGVYQD"
     gene            7708..8715
                     /locus_tag="VLL06_04090"
     CDS             7708..8715
                     /locus_tag="VLL06_04090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSS30178.1"
                     /translation="MGILIVDDSADDRLLLQSILSAAGYDQLLVAGSAAAAFRLLGLD
                     DGKHGAASRVDLILMDIVMPEMNGIEACRQIKAVERFRDTPIMMVTVKTDPVDLQLAF
                     AAGALDYIGKPINKIELLTRVRSVLRLVHEIDRRRAREQELLEVMRQLQEANQMLLRL
                     SCLDGLTGITNRRQFDDFLDQEWRRAVRELTPLSLIMLDIDSFKSYNDALGHTSGDEC
                     LRQVAAAISGAVNRPGDLVARYGGDEFVVVLPGTSTDGAAQVAETLRQRIESLGITHS
                     DGGCTTISVGFASTIPSRNTSPIDLIKAADQALYQAKQEGRNRAKQASTLSLVPHSHE
                     D"
     gene            8877..11981
                     /locus_tag="VLL06_04095"
     CDS             8877..11981
                     /locus_tag="VLL06_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS30179.1"
                     /translation="MAKKASPLRADRTAKRQPMLPAARRSGATQQSKRSRLSSVRAES
                     TEKQSRRTGRSQDINRTELALRQSEQRFRALLETTCDWVWEIDDHAVFTYSSPNLRDI
                     LGYEPREVLGKTPFDFMLPEEARRIGDLFGPIAAARQSFSHLESLYLHKDGRHILLEC
                     GGVPVFDSAGRFKGYHGIDRDITERKRVAEEARHNQALIVSIIENIPDMIFVKDAKTL
                     RFVRVNQAGEQLLGYPRENLIGKSDYDFFPKQEADSFTAMDRQVLQHGHLLDIPEEPV
                     ETRRKGKRLLHTKKVPINGADGTPQYLLGISEDITERKQIEQIERERARVLGQQQIAL
                     CELAKHDAVNKGNFEEALRMITETGSRVLEVERASIWMFSEQGTELELLDLYERTPNR
                     HITGYTLAAQDYPAYFQAIVDEEHAMAAHDAHTDARTREFSRSYLTPLGIGAMLDAPV
                     RRKGHVVGVLCHEHIGGPRSWTTEEVYFSSSLATFLTLALEARQRREAEQALVLAKEA
                     AEVASRAKSEFLASVSHEIRTPMNAIIGMADLLWETNLTPDQRKYLRIFRRAGGNLLS
                     LINDILDLSKVEVGHLELESTDFDLSDLVEKAIEILAMRANEKGLELACHLSPDVPRA
                     VIGDPNRLHQILINLISNAIKFTDSGSVTVRVMRDPELSLPGAIRFSISDTGIGIPSD
                     KLGAIFESFTQAHASVTRKYGGTGLGLTISKQLAELMNGRIWVESTLGTGSSFHCCVQ
                     LVVQSSPAFTHDNVQVSLQGVRTLVVDDHATNRLILRETLSALGAEVTDTASGHEAIG
                     EWRRAVTTERPYELLLLDCRMPEMDGFQVAEEIRRAGVPKGLTIVMLASDHWADDIAR
                     TYDMGLGGYLIKPIRKSDLLQTISIALDRSKGIQHSAGSAPVDTTSPTEVRALRILLV
                     EDSSDNQVLIRSYLKQTPHRLDIADHGGIALELFKNGHYDLILMDMQMPVMDGYEATK
                     AIRAWEREHDLPPTQVIALTALALKEDGVKILDAGCNAHMTKPLKKHTLLEVLQAYTS
                     RCVS"
     gene            11978..12307
                     /locus_tag="VLL06_04100"
     CDS             11978..12307
                     /locus_tag="VLL06_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HSS30180.1"
                     /translation="MIKEPNNNSENVITVYIDQGIEDIVPGFLENRRRDVQTLETALQ
                     HNDLKTIRVIGHRMKGDGGGYGFDAISVVGAALEQAAAREDRDGIRRSTAELIDFLAR
                     VTVVYRR"
     gene            complement(12596..>12972)
                     /locus_tag="VLL06_04105"
     CDS             complement(12596..>12972)
                     /locus_tag="VLL06_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS30181.1"
                     /translation="DTPEFRLLISTQLRSRNMTVFGAADIIQQAISTAREKRPDVILL
                     DIEMPGGDGFLVLERLKANKLLSTIPVIIVTARDPKEVESKALKVRAVSSLQKPVNID
                     NLMVVIEQALNTSHQTGGPQPA"
BASE COUNT         2885 a         3534 c         3549 g         3004 t
ORIGIN      
        1 ttcgtgagct gcagcccttt cccaaatcgc acaccgacgt ccgacatctc tctcacattc
       61 cacgatgcga gtgccctccg atgggcgcac atgagatcgg tccagaattc acccacacag
      121 cccgccacgt aataggtgta ccgatccaga tcatctattg tcttcagcgc aaccagatcc
      181 ttgactgatg tgcctgggaa gacgctgaga tccatttcca tgccctgggt gagtgtcgtc
      241 atgagtcgtt gcacacaacg ccgatcgtcc ggcgaaaatt cttggaataa tttaaaacag
      301 tcctccaatc gttcaagaag cacacgctcg gacgaatctc gctggagtgg tcccatggcc
      361 cgctggattt cacggatctg cccccatgaa atctgatcgc taacgaactg tcccttgagc
      421 tgacccagga agtccagtcg tcgcgggcga tctattaatt ccgtgtccgc aattgtgtcc
      481 gctgcgcggg caaacaggta ggccagactg acttgatcgc gcacgtctgc cggcacgacg
      541 accaacgttg tgtagaagag acgggatacg tgtctgagaa gctcgcggag cagttcgtgt
      601 ttggaggtct ttacgcttgt agccacatct atcgcacttc cagcgtccct tccatccctt
      661 tgtcgcgatg gctgggcaac ggccagagcc gtttgtcgca gtagatggga aatgtgccgg
      721 gctgaatcgg tgtaaatcta atggtaaccg ttttgccggc actcacatcc tgttcaacgg
      781 agagatttcc agttgggctc ttaatgatga agttgtgtgg gatgatcgtt gtcacactga
      841 tcagagccag ttcaaccggc ttcccagcct caacaatcaa atggtttggg gcatacgaat
      901 agctatcaag cgtaattgtg gctcgctgga ccccgtcttc cgctaccgga attaccattg
      961 gagggccgga ctgagagggc tccgctgcat ctacgattgt cacactgata gatatgatca
     1021 gcccgacggt cgccaagagg taggataatc gcatcggcgc cttctgcatg atccagttct
     1081 aacaggaagg atggaactgg tcaactttgt caaagagaat cagtcacttc gagcaccttg
     1141 accttggtaa ttacagagtt attttcctcg gaatatctct actgagatgc atttcagcga
     1201 atcggtcagt ataatcactc tcaccgatga ctcgtctgaa taacgagacg agagtctggt
     1261 cgcgaaggag gatctccatg aagtggatga aaggtatgtt cctactgttg gctgccaata
     1321 ttctgatcat ggtgacgctc tcgctcactg tgccaatact catcaacgtc gttctaccca
     1381 tattcggggt tgatctccga gggatggtcg acttgagttc gttagtctgg gccctcgcct
     1441 tcggctttgg aggcgccttt atcagtttgg cattctccaa acagatggct cgagccatgt
     1501 tggattgtcg ccagatcacg caaccgcggt cgcaagccga acaggtcatt tatggctctg
     1561 ttcaggagat cgcgcaacga cttctcatta cgatgcctga ggtgtggatc tatgactccc
     1621 ccgatcccaa cgccttcgcg accggcccga gcaagaacaa ttcaatggtg gccgtgtcga
     1681 ctggattatt gagcaatctc caagagcagg aagtgcgagc cgtcctcgcc cacgaaatgg
     1741 ggcatgtgta taacggcgac atgttcgcaa cgactgtgtt ggcggggctg atgaatacat
     1801 tcgtgtacta tattgctatg tgggtgcgtc ggttcttcgg agaacgcgat caagctatgc
     1861 tcgggtttgg gttgtcgatc gtgattcaga tcatcgttag tgtcatggcc tcggtcctga
     1921 tctgctggtt ctcgcgtcag cgtgaatttg gcgcggatgc cttttccgcc aaggtgtatg
     1981 gcaagaactc gatgatctcg gcccttcgtg cgattgatcg atgggtcacc cgttcgcagg
     2041 tcgagtactc cacgcaggac gcgctcgcga cgatgaagat ctcgggtaac tcgtcagggt
     2101 tcatgcgtct ctttgccaca catccgccga tggaagcacg cattgaagcc ttagaacgac
     2161 tctaaccgcg aggtgcctga tgaaacgatt gcgtcatacg ttggtgggat tgtctgtgac
     2221 gattggcttg atcgccggtg ttgggtcgag cgatatactt gagccgacct tcgcacaggc
     2281 ggaacaagaa gggactgtac tcatggcagc tcagacgccc atggtctatg actttacgct
     2341 gaatgatatc gaggggaagc cggtctcatt gagccagttc cgcggcaaag tactgttgtt
     2401 ggtcaacacg gccagcttct gcggtaacac gccgcaatac accgacctcg aaaaaatgta
     2461 cgagcagtat cgggagaagg gctttgagat cctggccttc cctgcgaaca actttgggca
     2521 gcaggagacg gggacggatg cagagatcaa aacgttctgt tacacgaaat gcagcctgtc
     2581 gtttccactc ttcagcaaga tcagcgtgaa gggcgacgac aaacatccgc tctaccgata
     2641 tctaactgaa cagagtcagt ttcccggcga agtggagtgg aacttccaga agtatttagt
     2701 cgatcgttca ggcaaggtcg tcgcccggta tcatcaccgc acgaagccgt tgacgccaga
     2761 ggtcgtgcag gatgtcgagc gggtactcgc ggccccttga cgctctcaag tttttcgata
     2821 aaatgaaata tgcacgcgat actcctggat cgcggcagcc agagactggc tgccgcgagg
     2881 gatattcgca tccagggcat cttcaatctc gggatcatca atctccacat catagcggtc
     2941 ttggatattg gtcctgacag gaccctgtgt cacctcccgc ggcatgaaaa tctctttcca
     3001 gacttctttc tcgaccagac atacgtctct gaatcgctca tagagcagcg tgagcttttc
     3061 ttgatccgtg actttaatgc gcgtttccat cctgatcatc gtctccatgt catgactgtg
     3121 gtcatcgcaa ctgcattcgc cactatccgg tatcgatgat gtactcgtag gtgggcatcg
     3181 gtccggttcg cgcgtgatcc catgagtcta cttgtgcgga ttcacgctga caggcgcagt
     3241 gtctgcgaca atggtaaacc gcatggtccc cacttgattg cccgcatgaa acgattgttg
     3301 cccaagcggc gtgatgaaca gttttacgag atacgtgccg acttcccagg tgtctccgga
     3361 cttcttgagt tccagaaatc cataacgttc atgccccggt acttccaggg tgtctttgcc
     3421 gagcggcata gatgagacga cacccttttc tccctctaga aaccattgtg cgctgaattg
     3481 ggtaggatct tctagagggg ctgactcgaa cacaatatat atcgcgggaa tatcccgagg
     3541 aaacaccgat cccggctcga tgggtttgag tcggggatct tgcgtgaacc agccttttcg
     3601 cccgaatgta tcccactcga tgtcataccc tttggccatc ttgatccagg taaagaggga
     3661 ttgcgggata cccttcttct gttcgtcgaa cgacggactt tgtgtgggat cgacctcggt
     3721 cgtgacttgt tctttcggcg cgaacagatc ccgcgcttcg acctcctgcc agacacacgc
     3781 gaccgtgctt agggcggcaa gtaggcttgc caggagcagt ttcctcagag gtctggtctc
     3841 aatcatctga atcatcatcg ctgtccactc gtaccatgat ggtatcgaga gatcatcatg
     3901 gggtcaacaa cagacggacc ggactgccca tctatttcac ggctgcgcac cttctgtgtt
     3961 acgctcaatc aaaactcctt cacagatgag gatcatgtgt ctctgacaca gccaggatat
     4021 atggacgagt ctgtgcctac cgacgtcaag ccgcagagtg caggtgagcg aggccgtctt
     4081 tacggcctcc gagacggtgc ctgccaggcc gtcacgcaag gaagcgggga acaatacctc
     4141 tctgcctttg ctctgctgct tcatgcgagt ccgttccaat tgagtgtctt gtcggcgttg
     4201 ccccaactta tcgggatggg ggctcaactc gtttccgtta agttattaca gtggttcccg
     4261 catcgcaaag tcctcatctc tgccggcacc attggtcaag ccttcacttg gattcccatc
     4321 gtgctcctgc cgctgctgct tccgcagtgg ggtccctggc ttctcgtagg tggagccgcc
     4381 gcctattttg cttgcgccca cttcaccatg ccggcttgga atagcttgat agccgattgg
     4441 ctcgaccagc atgaacgggg cgcctatttc gcacgccgag cgcaaatcat cgccattctc
     4501 agtttctttg cgctctgcgg agccggatgg gtgctgagcc tctggcaaga ttccactatg
     4561 gcctggtggg gatttgtggt gatctttgcc ttggccggaa gtgctcgcat cctctcggct
     4621 ttggccttgt caccagttca ggatgtgcat cagaccgctc accaggaggc ccagctagga
     4681 tttcgaggat ttctccgtca gaacatgaca aaggattttc gtcgattttt gctcttctcc
     4741 ggcctgatgc atgcgtccgt gttgatcgcc ggcccatttt tcgtcctgta catccttcaa
     4801 gatattcatc tgtcgtactt cggctacgga ggatggttgg cggccggcct tctgggtcaa
     4861 ctgctcactc tgcaggcctg gggccaattc ggagaccgtt tcggaaacaa agccctgttg
     4921 tccatcaccg gctttacggt gacgatcttg ccgatgttgt acctagtgag tacgaatgtc
     4981 ggctttctcc tggcagtgaa ttttctgggc ggcgtgatat gggctggcct ggcattgggg
     5041 ctgcaaaact atgtgttcga ttcggtgagg ccggaagacc gcgcaaaagc tgtcgcgttg
     5101 tacagcacgg tcaatgccat aggatggtcc gtgggggcct tgctagggag ctggctagtc
     5161 gagcacattc ccgcacgcat cgaatgggcg ggcatcatcc tccagcccgc atccaatttg
     5221 ccttttgtct tcttcctctc aggggttttg cggctactgg tctcgtccag ccttctgggc
     5281 accttccatg aagcgcgtca agtggagcga atacctcggc agcagcttct ctgggaattg
     5341 ccgctcgtga aacccgttgc ccagtttgtc gtccggactc tacccaaatc caacccgtag
     5401 ggtgccgcgc tagtaacctg gaacgggtgg ctgggcgggt cgctgttgtt cttcctgctt
     5461 gagcttgtcg aatgcgcgat ctgcgtgacc acgcacttgg tccggggtcg gctgcgcgag
     5521 tggttttggc tgatcgctgg aacaggccgt catggccatg accaggatcc cggtcatgac
     5581 gcacccgacg attttttgcc cacgtggtat ctcacgctct ctgtgctcca taccatgtcc
     5641 tccatgatga atgattgtga ttgtctcttc gctattcaaa tgcccgctga aggtcggaga
     5701 ccgtttgtat gatacctcgt tctagtgccg cccggctctg ttgctgatag tcccaattat
     5761 ccgatgggga ccagtcccaa cgttccccat catccatgcg agcggtcacc acgttgaaat
     5821 acactatttc ggcgctttcc actcgaataa tcttcgtcgt cacggtaatc ggtcgcacct
     5881 ggctgggtac atgagcgaat aggcgatccc gcaacgttgg cccatcgatc gagtagagca
     5941 gaacgctatc gactcccaac agatgtccga cccgtaccgc actgtcgtcg gctaccgctc
     6001 ccgcattctg gagccgctgc tcagtgatga ccgccagcaa atgaaagcga tcgaccactt
     6061 tgagtgtggg gcgcaggatt ttcaattgaa aggttgcgcc ttccagccgc tggtaggcct
     6121 ctagaaaatc agaattcgag gaacgggggt agaatatcgc gatccgttgc acatcaaccg
     6181 gaatggtcaa cgactgccca atggtccgaa cgacaagggg agatgcagcg gggagggaac
     6241 tgctctggca actggccaat gagagcgcca tcaggaagac catccctcct cgactgatta
     6301 cgcgaggcca attcatggtg gccctcttgg aagagccgag tgttgatgaa acagtccccg
     6361 attccctctt catcatacta ctccatcggc ttcaaaacca cttgactgtt ctgagtgcaa
     6421 caggctgatg atctcattga ctgggttgag gtgacgcggt attctcagca actcgattgt
     6481 ctgctccatt gaggtgctca tgtccctgca cgatcgtctc acagaagatc tgaagcgagc
     6541 catgaaggcc cgcgaccaac tgcggatgga cgtgattcgg atgatcaagg ccgccgtgat
     6601 gaataaggaa atggaaatca agaaggatct cgacgatgcc gaaatgagcc gtgtgatgac
     6661 gaccatgatc aagcaacgcc gggaatcggt cgaacagttt gagaaaggta accgggcaga
     6721 acttgctgcc aaagaacgac aggaaatcac cattctcgaa tcctatctcc ctcaggccct
     6781 ctctccggaa cagctctcca ccgtcgtgga tgcggtcatt caggaaactg gtgctcgatc
     6841 tctgaaagag atgggcgccg tgatgaaagc ggtgatggtc cgtgtggtgg gccaaccggt
     6901 agacggtaag cagatcagcg acctcgtccg cgccaagctc cagtagccca atcggcacct
     6961 gctatactca taggtgatgg ctcgcatacc ctgtgcgggc ggctgatagg aatcggcatg
     7021 ggcattctca tcatcgacga ttctccagac caacaggtcc tccttcgcgc aattcttggg
     7081 aaggccggcc atacggatct cctgagcgcg aactcggcaa agacggcgtt tcccatgctc
     7141 gacatggacg gccatgagcc gcccaatcag gtcgacttaa tactgatgga tgtcctcatg
     7201 ccggagctgg atggagtcga agcctgccgc cagattaaga gccgcccaca tctgtgcgat
     7261 attcccatca ttatggttac agccaaaagc gatctctcga atctccaggc cgcctttgcg
     7321 gcgggcgcga atgattacat cacgaaaccg gtgaacagtg tggaactcct ggcacgcgcc
     7381 tcctccgccc tggcgctgaa aaaggaaatg gactgccgca aagcgcgcga aggggagctc
     7441 cgtcggagca atgatgcgct gcagcaagcg ttacgggatg tcaaagtcct tagaggactt
     7501 atcccgatct gtgcatcctg taaaaagatt cgcaatgacg gaggattctg gcaacagttg
     7561 gaggaatata tcggcgaaca ttccgaggcc gaattcagcc acggtatctg tcagccatgt
     7621 gtcaaaaagc tttatcctgg agtttaccag gactagctgc tacgatttct caggcacacc
     7681 actcgtattc actggactca gaacactatg ggtattttga tcgttgatga ttcggctgac
     7741 gatcgcctct tgcttcagtc gatcctctcg gccgccggct atgaccagct cctggtggcc
     7801 ggctcggctg cagccgcatt ccgtttgctt gggcttgatg acgggaagca cggtgctgcc
     7861 tctcgagtcg acctgatcct catggacatc gtgatgccgg agatgaacgg catcgaagct
     7921 tgccgccaga tcaaggcggt tgaacgattc cgtgacacac cgatcatgat ggtcacggtg
     7981 aagaccgatc ccgttgatct ccaacttgcc tttgccgcag gcgcgctcga ctacattggg
     8041 aaacctatta acaaaatcga gctcctgact cgcgttcgat cagtattgcg attggtccat
     8101 gaaatcgacc ggcggcgggc tcgggagcag gagctccttg aggtcatgcg tcaactccaa
     8161 gaagcaaatc agatgctgct gcgcctgtcc tgtcttgatg gcctcaccgg cattaccaac
     8221 cgccgccaat ttgacgactt cttggaccag gaatggcgtc gtgctgtccg tgaattgaca
     8281 ccgctctctt tgatcatgct cgacatcgat agttttaagt cctacaacga tgccctcggc
     8341 catacgtcgg gagacgaatg tctccgtcag gtggccgctg ctatttcggg agccgtcaac
     8401 aggccaggag atctcgttgc tcgatacggc ggcgatgaat ttgtggttgt ccttcccggt
     8461 accagtacgg atggagccgc gcaggtggcg gaaacgcttc ggcaacgaat tgagtctctt
     8521 ggcattacgc attctgacgg tggctgcacc accatcagcg tgggctttgc cagtacgatt
     8581 cccagtcgga acacgtcccc cattgatttg atcaaggcgg cggatcaagc actgtatcaa
     8641 gccaagcagg aaggccgaaa tcgggccaaa caagccagca cactttctct cgtcccgcat
     8701 tctcacgaag actaaccgtc catttctcgc tatgaccatg ccagggcctc gacgagatgt
     8761 gcagatggca atcggcatga ccctcaccac gagtctctct gcaaacatct ttattctcct
     8821 atctccgaac tctctacacg atgcatatgc cgttgctggc gagagcgtgt gcggccatgg
     8881 ctaagaaagc gtcgccgctt cgagccgatc gcacggccaa acgccaacca atgctccctg
     8941 ctgcccgtcg ttcaggggcc acacagcagt ccaaacgatc acgactctct tctgttcgcg
     9001 cagaatccac ggagaaacag agtcgccgca cggggcgctc tcaagacatc aaccgaacgg
     9061 aactggcttt gcggcaaagt gagcagcggt ttcgtgcgtt gctggaaacg acatgcgatt
     9121 gggtctggga gatcgatgac cacgcggtgt tcacatacag cagtccaaac cttcgcgata
     9181 tcctcgggta tgaaccgagg gaagtgcttg gcaaaacacc gttcgatttc atgctgccgg
     9241 aagaggcccg tcgcatcggg gacctgtttg gccctatcgc ggcggctcgg caatcgttta
     9301 gccatctcga gtccctgtac ctgcataagg atggccgcca tatccttctt gaatgtggag
     9361 gagtgcctgt gtttgatagt gccggcaggt tcaagggata ccacggcatt gatcgagaca
     9421 tcacggaacg aaaacgagtc gcggaagagg caagacacaa tcaagcgctg atagtttcca
     9481 tcatcgaaaa tatccccgac atgatttttg tcaaggatgc caagaccctg aggtttgtgc
     9541 gcgtaaacca ggctggtgag caattgctgg gttatcccag agagaatctc attggcaaaa
     9601 gcgattacga cttctttccc aagcaggaag ccgattcctt cacagccatg gaccgccaag
     9661 tattgcaaca cggccacctc ctcgatattc cggaagaacc ggtcgaaact cggcgaaagg
     9721 ggaagcggct gcttcacacc aaaaaagttc cgatcaacgg cgctgatggt acgccccaat
     9781 atttgctagg catttcggag gacattaccg aacgcaaaca gatcgagcag atcgaacgag
     9841 agcgtgcgcg ggtattggga cagcaacaga ttgctttgtg cgagctggcg aagcacgatg
     9901 ccgtcaataa ggggaacttt gaagaagcgt tgcggatgat cactgaaacg ggaagccggg
     9961 tgctcgaagt ggaacgggcg agcatttgga tgttcagcga acaagggact gaattggaac
    10021 tgctcgatct ctacgaacgg accccgaacc ggcatatcac cggatacact ttggcggcac
    10081 aggattaccc ggcctacttt caagccatcg tagatgaaga gcacgcaatg gctgcccatg
    10141 atgctcatac ggatgccagg accagggagt tttcccgatc ctatctgacc ccgctcggca
    10201 tcggggccat gctggatgcg ccggttcggc gcaaggggca tgtcgtcggt gtgctctgtc
    10261 atgagcatat cggtggaccg cgatcctgga cgacggaaga agtatatttt tccagttcac
    10321 tggccacctt tttgaccctt gcactggaag cgcgtcagcg ccgggaggct gagcaagctc
    10381 ttgtgctcgc caaagaagcg gcagaagtcg ccagccgggc aaagagcgag tttttggcca
    10441 gtgtcagtca tgagatccgg acgcccatga atgcgataat cgggatggcg gatctgctgt
    10501 gggaaacgaa tttgactccc gatcaacgga agtacttgcg cattttccgc cgcgccggcg
    10561 gaaatttgtt gtctctgatt aacgatattc tcgacctgtc gaaagtcgaa gtcggccatc
    10621 tggagttgga atcgaccgac tttgacctga gcgacttggt ggaaaaagcc attgagattc
    10681 tggccatgcg agccaatgaa aaaggccttg agctcgcctg ccacctttcg cctgacgtcc
    10741 ctcgtgccgt gatcggtgac cccaaccgac tccaccagat cctcattaac ctcatcagca
    10801 acgcgatcaa atttaccgat agcggttccg tcacggtgcg agtgatgcgg gatccagaac
    10861 tctccttgcc cggcgccatt cgattttcta tcagtgatac agggatcggc attccgtccg
    10921 acaagctcgg tgctattttt gaaagcttta cgcaagcgca tgcctcagtg acgcgaaagt
    10981 atgggggaac cggcttaggc ctgaccatct caaagcaatt agccgagctc atgaacgggc
    11041 gaatctgggt ggagagtacg ctcggaactg gcagcagctt tcattgctgc gttcaactcg
    11101 tcgtgcaatc cagcccggca ttcacacacg acaacgtcca ggtcagtctt caaggggtcc
    11161 gaacccttgt agtcgatgac catgccacca atcgcctgat ccttcgtgaa accttgtctg
    11221 ccttgggagc agaggtgacg gatacggcgt ccggtcatga ggccatcgga gaatggcggc
    11281 gagcggttac gactgaacga ccctacgaac tactgttgct tgactgtcgc atgccggaaa
    11341 tggatgggtt tcaggtcgcc gaagaaatca gacgggccgg cgtgcccaag ggcctgacga
    11401 ttgtgatgct ggcctctgac cattgggccg acgatattgc ccgcacctac gacatggggc
    11461 tcggaggcta tttgatcaag cccatcagga aatcggatct gctccagacc atcagcatcg
    11521 ccctcgaccg ttctaagggc attcaacatt cggctggttc agcaccggtg gataccacat
    11581 ctcctaccga agtcagggct cttcgcatct tattggtcga agattcctcg gacaatcagg
    11641 tgctgatccg ttcctatttg aaacagactc ctcatcgcct tgatattgcg gatcacggag
    11701 gcattgcgtt ggaactattc aagaacggcc attacgacct catcctgatg gatatgcaaa
    11761 tgccggtgat ggatgggtat gaggctacaa aagcgatccg agcatgggaa agggagcatg
    11821 acctcccccc tactcaagtg atcgccttga cggctctggc actgaaagaa gacggggtga
    11881 agatactcga cgccggctgc aatgcccata tgaccaaacc cctcaaaaag catactctcc
    11941 tggaagtgct ccaggcctat acaagccggt gcgtatcatg attaaggaac cgaacaacaa
    12001 cagtgagaat gtcatcacgg tatacatcga ccagggcatt gaagacattg tcccaggatt
    12061 tctggagaat cgtcggcgtg atgtgcagac gctggaaaca gcattgcaac acaacgatct
    12121 gaagacaatc cgcgtcattg gtcaccgcat gaagggagat ggaggaggtt atgggttcga
    12181 tgcaatcagc gtcgtaggcg ctgctttgga acaagcggcg gcgagggaag accgagacgg
    12241 aatccgacgg tctaccgcag aactcatcga ttttcttgcc cgcgtcactg tggtgtaccg
    12301 gagataaata ttggaggcgc cgatccggcg agagtatttc atcaaccaat acgctggcga
    12361 tggcgcaagg aaagaaatcg tagataaaga gacatcattc gttggtatcc cctcctctgt
    12421 cttcacattc caggggttgc tccggcaacc tgaaatcgtc cgaccaatcg ttgaggaaat
    12481 tatctttgcc accatagtcc tgccatgggt gagttcaacc gcgagtattc gctggaatga
    12541 atccagcgtg gtgggatcct gaccctttcc aacccaagaa agaggaacga tagccctagg
    12601 cgggctgtgg cccgccggtt tggtgcgagg tgttcagggc ctgttcgatg acaaccatca
    12661 ggttgtcgat gttcaccggt ttttgaagag acgagacagc cctgactttg agggctttgc
    12721 tctccacctc cttgggatca cgagcggtca cgatgataac gggaatggtc gagagcaact
    12781 tgtttgcctt gaggcgttcg aggaccagga atccatcacc accaggcatt tcaatatcaa
    12841 gcagaatgac gtcgggccgc ttctcgcgcg ccgtgctgat cgcctgctga atgatgtcgg
    12901 cagccccgaa cacagtcatg tttcgagatc gcaactgtgt gctgatcagg agacgaaact
    12961 ctggggtatc gc
//
LOCUS       DATIPF010000157         4090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1002571, whole genome shotgun sequence.
ACCESSION   DATIPF010000157 DATIPF010000000
VERSION     DATIPF010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002571"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(389..856)
                     /gene="bcp"
                     /locus_tag="VLL06_04110"
     CDS             complement(389..856)
                     /gene="bcp"
                     /locus_tag="VLL06_04110"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HSS30182.1"
                     /translation="MAKELSIGDKAPALSLPDQSGEWVSLKDFTGKQVVLYFYPKDDT
                     PGCTKESCDFRDAITPMKKAGAVILGVSLDGKESHQKFIKKFDLPFTLLSDEDATVSK
                     AYGCYKEKNMYGRKYWGIERSTFVIDQAGKLKAIFRKVKVDGHVAEVQAVLKA"
     gene            complement(888..1331)
                     /locus_tag="VLL06_04115"
     CDS             complement(888..1331)
                     /locus_tag="VLL06_04115"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS30183.1"
                     /translation="MKYLSILGMILLLVGAVACSKSRAKPLVPLVLELGVKPQAEVLT
                     EQGTQAYRARQFDDAKNYFSQAVATAPQSGPAHYNYALALNALGDTEQARQQFMEAAN
                     LAPGDKVIWDSPALSPFGNVESPKKKLNQMPSTRQGLGGAGAGTR"
     gene            complement(1357..1632)
                     /locus_tag="VLL06_04120"
     CDS             complement(1357..1632)
                     /locus_tag="VLL06_04120"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HSS30184.1"
                     /translation="MLSEDKVSHLSHVILQAVKKSSLVTMQADEGRVLKEIKRVLASE
                     LAQEEEIDRKVKAKLASYSRGIVEGSAEWDVLYRKTFEEETRKHAKA"
     gene            complement(1716..1997)
                     /locus_tag="VLL06_04125"
     CDS             complement(1716..1997)
                     /locus_tag="VLL06_04125"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HSS30185.1"
                     /translation="MRLSKERVRHMSESLATRLQEEGHVVLAGDRKVFVEQIDHAILE
                     ELLVEDRLNAEVRQLLKAYEQDIERGHVDFQKMFTMVKSKLARERGIIL"
     gene            complement(2252..2635)
                     /locus_tag="VLL06_04130"
     CDS             complement(2252..2635)
                     /locus_tag="VLL06_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HSS30186.1"
                     /translation="MAALPNKIRITVGSVQLDAELKATKTAQEVYAVLPVESQVNTWG
                     EEFYFKLAGVKDHRETATNHVKVGDVAFWGAGQVLAIFFGRTPMSMGQDPVPADRVNV
                     IGHILGDASVLRRVMDVSTIRIERI"
     gene            2712..3548
                     /locus_tag="VLL06_04135"
     CDS             2712..3548
                     /locus_tag="VLL06_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HSS30187.1"
                     /translation="MKSRLQSRDHMLTSSFVLLKGIGQSSERRLWQDCILDWSAFLRS
                     PALPGISSTRKEWYDRELTTAQSRLEEGDAHYFGTCLKSREHWRLFEAFRHRTLYLDI
                     ETTGMSAREGHVTVVGLYRNGHMTSLIRGETLTEDRLHAELEQTDLFITFFGSGFDIP
                     YLQTKFPRLNFKKPHFDLCFAARRLGMQGGLKHIEHEVQIAREIDVVGLDGWEAVRLW
                     YQWCAGDEAARDLLLRYNAADTQNLEPLAALLYDQMVARFGPSSVGFPPTRYHQPVEA
                     AP"
     gene            3545..>4090
                     /locus_tag="VLL06_04140"
     CDS             3545..>4090
                     /locus_tag="VLL06_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HSS30188.1"
                     /translation="MIQPQQWTGVGRTERGHVRATNQDALALLNDCGVWIVADGMGGH
                     PAGDLAAQTAVSVATQRAREREAWLREHPNAAGEFVADLVTSANQRIHDLMLAKPSLQ
                     GMGTTFVAFTITPAPMPSAHIAHLGDSRAYLYRTGALKQLTRDHTLVEKYVQRGLIDA
                     ATALTHPERHILTKGLGMGVDM"
BASE COUNT          888 a         1218 c         1061 g          923 t
ORIGIN      
        1 gttcttccct gtgagcctca cttcaatggt gaccggctgg ttgggtgcgg tgagactatc
       61 gtgaaccgtc aaggttccga ctgttttctc cgccgcgaag aggatggagg ttgcagtcaa
      121 aaaaaggagg gtggtgaaaa caccggctct gcggaaagaa acacccatag gccgcacagc
      181 cacacaatga gtctggggaa acactatata tggtgcgacg gaatcgccca tacatctaaa
      241 acgaaggtac agaacaggat gctcaaaaag ctcgtccagc aaggccgcag tcgaagaaag
      301 caccggagac gtagcctctc ggctacgttg aggatgcttt cgaggcgaga acgccgctgg
      361 cgggcttttt cagcatcctg aaaacaggtt aggctttcag aaccgcttgg acttctgcta
      421 catgaccgtc gaccttgacc ttgcgaaaaa tggccttcag tttgccggct tggtcgatca
      481 caaaggtgct gcgttcgatt ccccaatact ttctgccgta catatttttc tctttgtaac
      541 aaccgtaggc cttcgagacc gtggcatctt cgtcgctcag cagcgtgaag gggaggtcga
      601 acttcttgat gaacttctgg tgagactctt tgccgtccag gctcacaccg agaatcacgg
      661 ctcccgcctt cttcatcgga gtgatggcat cgcgaaagtc gcacgactct ttggtacagc
      721 cgggtgtatc atctttgggg taaaaataga gcaccacttg cttacccgta aaatctttca
      781 ggctgaccca ttctcccgat tggtcgggaa gcgacagagc gggagccttg tcaccgatac
      841 tgagttcctt tgccatatca acgcaccctt tctctggttg tctgcaacta acgagtcccg
      901 gcgccggcgc cgcccaagcc ctggcgcgta ctcggcattt ggtttaattt ctttttggga
      961 gactcgacat tgccgaacgg gctgagcgcg ggcgaatccc agatcacttt gtcgcccgga
     1021 gccaagttgg ccgcttccat gaactgttgg cgcgcttgct ctgtgtcacc cagggcattc
     1081 aatgccaatg cgtagttata gtgcgcaggc cccgattgtg gagccgtggc tactgcttgg
     1141 gaaaagtaat ttttcgcgtc gtcgaattgc ctcgctcgat acgcctgtgt cccctgttcg
     1201 gtcaagacct ctgcttgcgg cttcactccc aattcgagca ccagcggaac caacggcttc
     1261 gcccgactct tcgaacaggc gaccgccccc acgagtaaca gaatcatccc cagtatagac
     1321 aggtatttca tggtacctcc atgcgtcata gacacctcat gccttcgcat gtttccgtgt
     1381 ctcttcctcg aatgttttcc gatagagcac gtcccactct gcactgccct cgacgatccc
     1441 gcgtgagtaa gaggccagct tggctttgac ctttcgatct atctcttctt cttgcgccaa
     1501 ctcagatgcc agtacccgct tgatctcttt caggacccgc ccctcgtcgg cctgcatggt
     1561 caccagtgag ctcttcttca cggcctgaag aatgacgtgg gagagatgac tgactttgtc
     1621 ttcgcttaac ataactcatc cgtgaaacat cacacgcata gtgtcaggcg taccacggtg
     1681 acacatcaat catgtttcgt gtcacgtttc acgctctaga gaatgatccc tcgttcgcgc
     1741 gccagcttac tcttcaccat cgtgaacatc ttctggaagt ccacgtgtcc ccgttcgata
     1801 tcttgctcgt aggctttcaa cagctgccgc acttcggcgt tcaagcggtc ttcgaccaac
     1861 agttcctcga gaatggcatg atcaatttgc tcgacaaaca ctttacgatc cccggcgagc
     1921 acgacatgcc cctcctcctg taacctggtg gcaagcgatt ccgacatgtg gcgaacgcgt
     1981 tctttcgata gtcgcatcgt caatgatcca tggagaaacc aattagagca tggtcccgta
     2041 gaatgctcaa acagtccaac cttctcaccc acccagccca ggcgcgccga gacgcgcctt
     2101 atcccaagca aggccgcagc gagttatcac gtttaagggg tggctgggat aatcccaact
     2161 gcgcgcgtcc aacgaaggcc ttctgaggcc gcgcgttgcg cgagcacggg gatcgtccca
     2221 gctacccccc cgctcctttt tcagcattct actagatccg ttcgattcga atggtcgata
     2281 catccatgac acgccgcagt accgacgcat ccccaagaat atggccaatg acattcacac
     2341 ggtcagccgg aaccggatcc tgccccatac tcattggtgt gcgcccgaag aaaatggcca
     2401 ggacctgccc cgctccccag aacgcgacat cgccgacttt gacgtgattt gttgccgtct
     2461 ctctatgatc tttcactccg gccagcttaa agtagaactc ttcgccccag gtgttgacct
     2521 gactttcaac cggtaacacc gcgtacactt cctgagccgt tttcgttgcc ttaagttctg
     2581 cgtccagctg cacggaacca acggtgatac gaattttgtt ggggagagca gccatggaat
     2641 tctactcggc gcctccaggc agccatagga tgaaaacccg ctcaggtcgc ggactttagc
     2701 ttgtttattc gatgaaatca aggctacaat cccgcgatca tatgctcacg tcatccttcg
     2761 tgcttctcaa aggcatcggt caaagttccg aacgtcgtct ctggcaggac tgcattctgg
     2821 attggtccgc attcctccga tctccggccc tgcccggtat ttcctcaacc agaaaagagt
     2881 ggtatgaccg cgaactcacc accgcccagt cacgcctgga agaaggcgac gcgcactact
     2941 tcggtacctg cctgaagagt cgtgagcact ggcgcctctt cgaagcgttt cgtcatcgca
     3001 cgctttatct ggacatcgaa acgaccggga tgtctgcgcg agaaggtcat gtgacggtgg
     3061 tcggattgta tcgcaacgga cacatgacga gcctaattcg cggagaaacc ttaacggaag
     3121 accgattgca cgcagagctg gaacagacag acctcttcat tacgttcttc ggcagcgggt
     3181 ttgacattcc ttacttgcag acgaagttcc cccggttgaa ttttaagaaa ccgcacttcg
     3241 atctctgctt tgcggcgcgc cggctgggta tgcagggcgg tctgaaacac atcgagcacg
     3301 aagtccagat tgcacgcgag atcgacgtgg tgggactcga cggatgggaa gccgttcgcc
     3361 tgtggtacca atggtgcgcg ggagacgagg ccgcacgcga tctcttgctg aggtacaacg
     3421 ccgccgatac tcaaaatctt gaaccgctcg cggcattgct ctatgaccaa atggtcgcgc
     3481 gcttcggccc atcttctgtt ggatttccgc cgacccgtta ccaccagccg gtcgaggccg
     3541 ctccgtgata caaccccagc agtggaccgg ggtcggtcga accgaaaggg gccatgttcg
     3601 agcgacgaat caagacgcgc tggctctcct gaacgactgc ggtgtctgga ttgtggcaga
     3661 cggaatggga ggtcatccag caggggatct cgcagcccaa acggccgtct ccgtggccac
     3721 acagcgggca cgagaacgag aggcctggct ccgcgagcac ccgaacgctg ccggcgaatt
     3781 tgtcgcagat ctcgtgacga gcgccaacca gaggatccat gacctcatgc tcgcgaaacc
     3841 gtccttgcaa gggatgggca cgaccttcgt tgcgttcacg atcacaccgg caccaatgcc
     3901 atctgcccat atcgcccact tgggagatag ccgagcctac ctctatcgga cgggagcgct
     3961 caagcaattg acgcgtgacc ataccctcgt agaaaaatat gtgcagcgcg gcctgatcga
     4021 tgcggccacg gcactcacac atcctgagcg tcacattttg accaaaggct tggggatggg
     4081 cgtcgacatg
//
LOCUS       DATIPF010000158         3533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1180892, whole genome shotgun sequence.
ACCESSION   DATIPF010000158 DATIPF010000000
VERSION     DATIPF010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180892"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VLL06_04145"
     CDS             <1..267
                     /locus_tag="VLL06_04145"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSS30189.1"
                     /translation="ATGTNPAAAPGGFAPAAVNGVGTFNTATGGGSTQTLWEITNTLQ
                     YKPAPSLITRVEVRHDHSNRNSFLNGTVATNNQTTLGFQVIYLF"
     gene            680..2026
                     /locus_tag="VLL06_04150"
     CDS             680..2026
                     /locus_tag="VLL06_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HSS30190.1"
                     /translation="MKKILPVLLICLVMVAGWTALSVQDVVAQAPEAPAAPAELKADP
                     GDTAWILASSALVMLMTLPGLALFYGGLTRQKNILSTLLHSFICLCLGSMAWVVFGYS
                     LSFGPDVGGVIGNLDWFMLKGVDVTPHPTYGPTIPHELFMVFQLMFAAITPALITGAL
                     AERIRFSSMLWFLGIWSVVVYYPVAHWVWGGGWLGAMGAADFAGGTVVHVTCGMAALV
                     ACIMLGKRNGYPNTPMPPHNLPLTVLGAGLLWFGWFGFNAGSSLGANAQAVGAFVATH
                     IAASTATVVWMLIEWAHRGKPTALGVATGTIAGLATITPVAGYVSLFPAFIVGLTAAI
                     ICYIFVVFIKSAAGYDDALDVVGVHGVGGATGCILGGLFNSKAMGAAADGVFYGGGFG
                     LMGAQITMVLAVGAYTAIGTWIILKIIDVIIGIRVTGDEEQEGLDVSQHGEKAYHV"
     gene            2126..2464
                     /locus_tag="VLL06_04155"
     CDS             2126..2464
                     /locus_tag="VLL06_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HSS30191.1"
                     /translation="MKMIEAIVKPFKLDDIKDALVEIGVQGMTVSEVKGFGRQKGSHE
                     TYRGAEYTTDFVPKVKIELYVADNMVQRVLETITRAAKTGSVGDGKIFVSDLESVLRI
                     RTGETGESAL"
     gene            2467..>3533
                     /locus_tag="VLL06_04160"
     CDS             2467..>3533
                     /locus_tag="VLL06_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30192.1"
                     /translation="MPPGNGSPAELLRKAEFEPVSALAEYRLALRQRLLAGASGADVM
                     TALTEFVDRLIIARYKKAMRKSGEEAQVAGDQYCCLVALGGYGFRELAPYSDIDVMFL
                     FREEAGESAPALFKEVLHHLWDLGFQVGHSMRTIHDCIDLAGKDLTIRTSMMSARFLT
                     GSPYLFQEFNRRYAQEVVGSEVGKFIEQKVAERDREYVKFGQTVYLLEPNVKKSKGGL
                     RDFHLLRWAGMARFHPATAGPAVVLMEHEVANDVHAPERHGLRYGPLHRAAGWSYGEA
                     FDSPPEGARGVVARQAGVGMHIADDVYRIRQLPQANHLRVETHGNIDMVFPGQEKDRV
                     TPRTEFAVLLHSIDLIDLLLDL"
BASE COUNT          732 a          903 c         1123 g          775 t
ORIGIN      
        1 gcgactggta ctaatcctgc tgccgctcca ggtggttttg cgcctgcagc agtaaatggt
       61 gttggtacat tcaacactgc aaccggtggc ggatcgactc agaccctctg ggagattacc
      121 aatacacttc aatacaagcc ggctccgtca ctcatcacga gagtggaggt tcggcatgac
      181 cattcaaaca gaaattcgtt cctgaatggc acggtcgcaa cgaacaatca aacaaccctc
      241 ggattccaag tcatctatct gttctaagcg aggggatcag ggaattgtgc tgacagttga
      301 agtgtagttc ctgtattgag ctcggtcgaa gcgcctccgt ggcatgggcc acggaggcgc
      361 tcacgatagg agccaccaac cgcagacagg tagaagtaag gttggcggga ttgagggtga
      421 tctcgtgcaa ggaacagatg attcagacgc tggttcacta atccactcgg gtaggccgat
      481 ccttcgacag attcaagata gcgtaagacg gagcaccgta tgcttcgatg gagacgcctg
      541 tcctaaatcg gggcgaagga gtcgcgtgag gcaccatcgg gggccgctca aacatttcga
      601 ccaaacgtat cgacgtattg acaacctcgt cctgaccagg ggatgaaagg atgcaaatat
      661 gctatcggct atgagtaata tgaaaaagat tctgccggtc cttctgatct gccttgtcat
      721 ggtggcagga tggacggctc tctctgtaca agatgtcgtt gcgcaggctc cagaggcgcc
      781 ggccgctccg gctgagctga aggcggatcc gggggacacg gcatggatcc ttgcctcctc
      841 ggcgctggtg atgttgatga cattacccgg gttggcgctc ttctatggag gactcaccag
      901 gcaaaaaaac atcctgagta cgctcttgca tagctttatc tgtctctgcc taggcagtat
      961 ggcttgggtg gtgttcgggt acagcctatc gtttggtccc gatgtgggag gggtaattgg
     1021 aaatctcgat tggttcatgt tgaagggggt cgatgtcact ccccatccga cctatggtcc
     1081 cacgattccc cacgaactct tcatggtctt ccagctgatg ttcgccgcta ttactccggc
     1141 cctgatcaca ggcgcccttg cggaacgtat cagattttcc tcaatgctgt ggtttctcgg
     1201 tatctggtcc gtggtggtgt attaccctgt ggcccattgg gtctggggcg gcggatggtt
     1261 gggggcaatg ggcgccgcag acttcgccgg aggtacggtg gtgcacgtca cgtgcggaat
     1321 ggctgcgctg gtggcctgca tcatgctggg aaaacgaaat ggctatccga acaccccgat
     1381 gcctcctcat aaccttcccc tcacggtact gggggctggt ctactctggt tcggctggtt
     1441 cgggttcaat gccggcagct cgttgggggc gaatgcgcag gcggtcggtg cctttgtggc
     1501 gacccatatc gcagcttcta ctgcgacggt ggtatggatg ctgatcgaat gggcgcatcg
     1561 tgggaagccc actgcgctcg gtgtggctac cggaactatt gccgggctgg caaccattac
     1621 tcctgtagcc gggtatgtct ccctgttccc ggcatttatc gtgggtctca cggccgcgat
     1681 tatctgttat atcttcgtgg tcttcataaa gagcgccgcg ggttacgatg atgcgcttga
     1741 tgtcgtgggg gtccacggtg ttggtggagc aaccggctgc atcctgggag ggttgtttaa
     1801 ttcaaaggca atgggtgccg cggccgatgg ggtgttctat ggcggaggct tcggactcat
     1861 gggggctcag atcacgatgg tcctcgcggt gggggcctat acggcgatcg gcacctggat
     1921 cattctcaag atcatcgatg tgatcatcgg cattcgtgtg acgggagatg aagaacaaga
     1981 aggcttggat gtgagccagc acggtgagaa agcctatcac gtttagtaga tggaccatgg
     2041 tacaatcgac gaaaccactg tgttggcagc cccggcgacg cggacgatcc gcgtcgccgg
     2101 gggagcctga gagggaggca gcactatgaa gatgattgaa gccatcgtga agccgttcaa
     2161 gttggatgac atcaaggatg cgttggtgga aataggggtg caggggatga cggtgtcgga
     2221 agtcaaagga ttcggacgcc agaaggggag tcacgagacc tatcggggcg ccgagtatac
     2281 gaccgacttt gtgccgaagg tgaagatcga actgtatgtc gcggacaaca tggtgcagcg
     2341 cgtgctcgaa accattacgc gcgccgcgaa gaccggcagc gtcggggacg ggaagatttt
     2401 cgtgtcggat ctcgaatccg tgttgcggat caggaccggc gaaacggggg aaagcgcact
     2461 gtgacaatgc cacctggcaa tgggtcgccg gccgaactct tacgcaaggc agagttcgag
     2521 ccggtctccg ccctagccga gtaccgattg gcgctccggc agcggttgct ggccggggcc
     2581 tcgggggccg atgtcatgac ggccctgacc gagttcgtgg accggctgat tattgcccgg
     2641 tacaagaaag ccatgcggaa gtccggggag gaagcgcagg tcgcggggga ccagtactgt
     2701 tgcctggtgg ccttgggagg ctatggattc cgggagctgg ctccctattc agatatcgac
     2761 gtcatgttcc tctttcgcga agaggcaggc gaatcggcac cggccctgtt caaggaggtg
     2821 ctccaccatc tgtgggatct aggattccag gtcggccaca gcatgcgaac gattcacgat
     2881 tgcatcgatt tggcagggaa ggacctcacg attcgcacgt ccatgatgtc cgccagattt
     2941 ttgaccggaa gtccctacct cttccaagag tttaatcgcc gctatgcgca ggaagtggtc
     3001 gggagtgagg tggggaagtt tattgagcaa aaggtagcgg agcgcgaccg tgaatatgtg
     3061 aaatttggac aaacggtgta tcttctagag cccaatgtga agaagagcaa aggtgggctg
     3121 cgggatttcc atctgcttcg gtgggccggc atggccaggt tccatccagc caccgctggc
     3181 ccagccgtgg ttctgatgga gcacgaggtc gccaatgacg tgcatgcgcc ggaacggcac
     3241 ggcctgcgat acggcccact gcatcgtgcc gccggttgga gttacggcga agccttcgac
     3301 tccccgccag aaggtgcacg tggcgttgtt gcgaggcagg ctggcgtcgg aatgcacatt
     3361 gccgacgatg tgtaccgcat ccggcaattg ccccaggcca accacttgcg tgtagaaacc
     3421 cacggaaata tcgacatggt attccccggg caggaaaaag atcgcgttac gccgcgaacc
     3481 gaattcgctg tgctgctgca cagcatagat cttatcgatc tgctcctgga tct
//
LOCUS       DATIPF010000159         4116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_471789, whole genome shotgun sequence.
ACCESSION   DATIPF010000159 DATIPF010000000
VERSION     DATIPF010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471789"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VLL06_04165"
     CDS             complement(<1..241)
                     /locus_tag="VLL06_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30193.1"
                     /translation="MKKNLFTTPEPEELVTITKDVDADDPEASDLLDVIGRETSEEEP
                     PEELTKKVSRTAVSGGPFMLESLYFRSFGERALLTR"
     gene            831..1700
                     /locus_tag="VLL06_04170"
     CDS             831..1700
                     /locus_tag="VLL06_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HSS30194.1"
                     /translation="MSHFQSGERIHLVDKKGRQYALTLKAGDLYQLSGHKIAHDDLIG
                     KPDGSLVMLSGNKTMLALKPTFGDYVLKMPRGAQVLYPKDLALIPMWADVYPGARVFE
                     AGTGSGALTMALLRAVGPRGLVVTYEARDDFARTAMMNIERYMGPVPNLIPLCRNVYE
                     GISVLDDGLPFDRLVLDLPEPWQVVPHAAQVLRSGGMYLSFVPTIPQVVQTVEALERA
                     TVFGMIETFETLLRTWSIQGRSIRPDHRMVAHSGFITVGRKIESGLWPAAQVVESVGA
                     TTDVHEEREDPAR"
     gene            1697..2452
                     /locus_tag="VLL06_04175"
     CDS             1697..2452
                     /locus_tag="VLL06_04175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSS30195.1"
                     /translation="MNRLRGKVAVVTGGNAGIGEAIAKAFAREGASVVITGRRQGELD
                     RVVNDIAKEHGKAFAIAGSVTDEFHVQETVRQTVQQFGRLDILVNNAGVGDFGKRLHE
                     IDDVTWAHVLDVNLGGVFRMTRAALSQMLKQGKGAIVNISSIASLVGLPTLPVYAASK
                     GALDAITRALAVDYAKEGIRCNVVNPGLIDTPMAAPLMSNSEQLDPILSHYPIRRAGK
                     PEEVANMVLFLASDEAAWVTGGTFPIDGGMTIS"
     gene            2549..3400
                     /locus_tag="VLL06_04180"
     CDS             2549..3400
                     /locus_tag="VLL06_04180"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HSS30196.1"
                     /translation="MDINAHTQFCGVIGNPVEHSLSPAIHNAAFQKLGLNFVYLAFRV
                     DAIGAAIKGFRALGNFRGASVTIPHKVASVPFLDSIAPTARHIGAINTIVAADGILIG
                     YNTDATGALRALREGSVALEGQKVVMLGSGGAARAIAFALGTEAGIKHLTILGIDDQE
                     RTALARDLQSKTAMVVHESCLDESTLHKVLPDTHVLIHCTPMGMSPNVHETSVPAMLL
                     HAGLTVMDIVYNPRDTQLLKDAKAAGCRTIPGLEMFLHQAAAQFELWTNQTAPVDVMR
                     SVLESRF"
     gene            3400..3900
                     /locus_tag="VLL06_04185"
     CDS             3400..3900
                     /locus_tag="VLL06_04185"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HSS30197.1"
                     /translation="MNIVLMGYRGTGKSTVGRLLAARLGRELVSTDAEIVKRAQHSIP
                     EIVAQKGWEHFRDLESDICREFASRDQLVIDTGGGAILRVQNVEALKKNGTVFWLTAS
                     VEIIAKRIGSSNQRPSLTGTKSFVDEIQDVLRERTPKYQAAADHSIATDNRSVNQLVE
                     TILTLI"
     gene            3970..4045
                     /locus_tag="VLL06_04190"
     tRNA            3970..4045
                     /locus_tag="VLL06_04190"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4003..4005,aa:Arg,seq:tcg)
BASE COUNT          900 a         1044 c         1184 g          988 t
ORIGIN      
        1 ctctggtcaa tagggcacgt tcgccgaagg aacgaaaata gagtgactcc aacatgaatg
       61 ggccgccact cacagcagta cgggagacct tcttcgtcag ttcctctggt ggctcctctt
      121 cggaggtttc tcggccgatg acatccaaga ggtcgcttgc ctcaggatcg tccgcatcaa
      181 catcctttgt aatcgtgacc agttcttccg gctcaggggt tgtgaataga ttctttttca
      241 tctcatctcc cctcctctga ctgtctgatt cgttgctgcc catggttgtg acctcaacag
      301 actcctgttc tattttatta gagacagctt tcccccaaac gattcattct tggctctgcg
      361 ctgttctgat atctctctac gcagcaaatc ctgtgctgga tcgcatataa gaacggcgta
      421 tcgcgatcac gaggaaaaga cagcactatt aaagtattct aatgaggaga tttgaaatgg
      481 agtgaggcaa ccttgctgca gatgtgtagg gaaatctata agcaataggc ccaatcgcca
      541 catcattcta tttcagggcc aattcttaca gaaaggggac gatcgcatcg aagggaatga
      601 gaaaattgtg ctggagttta tccgacgcga attcaacgag cgtcagattg cccagcacat
      661 gcgtcgggtt ttcaagcaat cacgtcagcc gtatcgtcga ggtgttgaga ggcttcgaat
      721 tcgctgtcag cgtgacggct ttcgcatgat gtttgggagg ccaacaattc catcactgtc
      781 gttgttgtct tgttgaaaac tcggcgtcgc gcttctagaa tgaatccccg atgtcacatt
      841 ttcagtcagg cgaacgcatc cacctcgtcg acaagaaagg gcggcagtat gccttgaccc
      901 tgaaggctgg agatctctac cagttgagtg gtcacaaaat tgcccacgac gacctcattg
      961 gaaagcccga tggctctctg gtcatgcttt caggcaacaa gaccatgttg gcgctcaagc
     1021 ccacgtttgg cgattatgtg ctgaagatgc cgcgtggcgc gcaggtattg tacccgaagg
     1081 atcttgcgct tattcccatg tgggcggacg tctatcctgg tgcacgcgtg ttcgaagcgg
     1141 ggaccggttc cggagcttta acgatggccc tcttgcgtgc tgtgggccca cggggcttgg
     1201 tggtgaccta cgaagcccgt gatgatttcg cgcgcacagc gatgatgaat attgaacgat
     1261 acatgggccc agtgccgaat ctcatcccgc tttgccgaaa cgtatacgag gggatcagtg
     1321 tgctcgacga tggcctgcct ttcgatcgtc tcgtcctcga tcttcccgag ccgtggcagg
     1381 tggtacctca tgccgcacag gtgcttcgtt ctggtggcat gtatttgagc tttgttccca
     1441 ctattcctca agtcgtacag acggttgagg cccttgagcg agcgacggtc tttggcatga
     1501 tcgaaacatt tgaaacgttg ctgaggactt ggtcgattca gggacgaagc atacgacccg
     1561 atcatcgaat ggtcgcacat tcaggattca tcacagtcgg caggaaaatc gaatctggct
     1621 tgtggcccgc cgctcaagtt gttgagtccg taggcgcgac tactgatgtt cacgaagaga
     1681 gggaggatcc tgcgcgatga atcggttacg tggaaaggtt gcggtcgtca cgggaggtaa
     1741 cgccggtatc ggtgaagcga tcgcgaaagc ctttgcgcgc gaaggggctt ctgtcgtgat
     1801 cacggggcgg cgacagggag agttggaccg ggttgtgaac gacattgcga aggagcatgg
     1861 caaagccttt gccattgccg gatcggtcac agatgaattc catgttcagg agacagtgcg
     1921 tcagacggtg cagcagtttg ggcggctcga catccttgtc aacaatgccg gagtaggaga
     1981 ctttggaaag cgcctgcacg agatcgacga tgtgacctgg gcgcacgttc ttgacgtcaa
     2041 cttgggcggg gtgttccgga tgacgcgagc cgccctatcg cagatgttga agcaggggaa
     2101 gggagcaatc gtcaatatct cttcgatcgc gagcctggtt gggcttccca cattgccggt
     2161 ttatgccgct tccaagggtg cactcgatgc cattacaaga gccctggcag ttgattatgc
     2221 caaggagggc attcgctgca atgtggtgaa tcctggactc atcgatacgc cgatggccgc
     2281 tccgttgatg agcaactccg agcagttgga ccccattctg tcgcactatc ctatccgccg
     2341 agccgggaag ccagaagagg tggctaatat ggttctcttt ctggcctccg acgaagcagc
     2401 ctgggtcaca ggcgggacct ttcctatcga tggaggaatg acgatctcgt gagcagtcgt
     2461 ccctgcgaac ggtgatggcc gcgctgacgg caatgggtgt ctgtcagttg gtccgaagac
     2521 gatatagcat tcgccacttc ttgaacctat ggacatcaat gcacataccc agttctgcgg
     2581 tgtcatcggt aatccggttg agcattcgct ctcgcccgca attcacaacg ccgcattcca
     2641 gaaactcggg ctgaactttg tgtatctggc gttccgtgta gatgcgattg gcgctgcgat
     2701 caagggtttt cgcgctctgg ggaattttcg cggtgcgagc gtgaccatcc cccacaaagt
     2761 ggcatccgtt ccgtttcttg actctatcgc gccgactgca cgtcatattg gagccatcaa
     2821 taccattgtg gccgcggacg ggatcctcat aggctataac accgatgcga ccggcgcctt
     2881 gcgcgcgctt cgtgaaggct cggttgcctt ggaaggacag aaagtggtca tgctcgggtc
     2941 tggaggtgct gctcgcgcga ttgcctttgc actcgggaca gaggctggga tcaagcactt
     3001 gaccatactt gggatcgatg accaggaacg tacagccttg gcacgagatc tccagtcaaa
     3061 gacagcgatg gtcgtacatg aatcatgtct tgatgagagt acgttgcaca aggtcttgcc
     3121 agatactcat gtgctgatcc attgcacacc aatgggtatg tcgcccaatg tgcatgagac
     3181 ttctgtgcct gcgatgctgc tccatgcggg actgacagtc atggatattg tctataaccc
     3241 tcgggacacc cagttattga aagatgccaa ggcggcaggt tgtcgcacaa tccccgggct
     3301 cgagatgttt cttcatcaag ctgcggcgca gttcgagctc tggaccaatc agactgctcc
     3361 agtcgatgtg atgcgctccg ttctggagtc ccgcttctga tgaatatcgt gctgatgggc
     3421 tatcggggga ccggcaagag tacggtgggc aggctgcttg cggcgcgatt gggacgagag
     3481 ctggtgtcaa ccgatgcaga gatcgtcaaa cgtgcccagc actcgattcc tgagatcgtt
     3541 gcccaaaagg gatgggaaca ctttcgcgat ctggagtccg atatttgccg agaattcgcc
     3601 agccgtgacc agcttgtgat tgataccggc ggtggggcca tacttagggt gcagaatgtc
     3661 gaggcgttga agaagaatgg tacggtattt tggctgacgg cttccgttga aataatcgca
     3721 aaacgaatcg gtagtagcaa tcaacgtccc tcgttgacgg ggaccaagtc gttcgtcgat
     3781 gaaattcagg acgtgttacg ggaacggacg ccgaaatatc aagcggcggc cgaccatagt
     3841 atcgctaccg ataatcgatc ggtcaatcag ctggttgaga cgatacttac gctgatatga
     3901 gcaggatgag gttgtaccgt agcttcgtcc gtgacaaggc ctcagtcgaa gtgtttgaag
     3961 tagccgcctg cgcccgtagc tcaatggata gagcatcgga cttcggatcc gagggttggg
     4021 ggttcaagtc cctccgggcg caccacttca tccgtttaat cctcagggcg gaatcaatca
     4081 agtatcagta gcgtacaact gtgtacaact gatcta
//
LOCUS       DATIPF010000160         4089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_118302, whole genome shotgun sequence.
ACCESSION   DATIPF010000160 DATIPF010000000
VERSION     DATIPF010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118302"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VLL06_04195"
     CDS             <1..589
                     /locus_tag="VLL06_04195"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HSS30198.1"
                     /translation="ELLRPLLADITADGLSLVGEATAEELDLTEDDAVVVGPLAVSLE
                     LTNVEGLVAVTGVLEGMIVRECVRCLKEYEDPLAFSVRAAFIPEPKSAPRHPKRVDLR
                     KAHTKIVEAEQEEEPDDQYQYQGNQLELAPMLREHVILSAPMQPLCNDDCLGLCARCG
                     KNLNEGPCQCAEERPIPTVRVVQVTNRKTGGSSTP"
     gene            627..812
                     /gene="rpmF"
                     /locus_tag="VLL06_04200"
     CDS             627..812
                     /gene="rpmF"
                     /locus_tag="VLL06_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HSS30199.1"
                     /translation="MPNPKHKHSRARRDKRRTQKLRITPPGMSVCPQCHEIKLPHYTC
                     LNCGTYKGKAVIQVEES"
     gene            complement(809..967)
                     /locus_tag="VLL06_04205"
     CDS             complement(809..967)
                     /locus_tag="VLL06_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30200.1"
                     /translation="MVTKDEQPSPHDMRQEFRAVTELTQQGTKHRPIVIHWAMGPGGA
                     QRLNHKPN"
     gene            1025..2029
                     /gene="plsX"
                     /locus_tag="VLL06_04210"
     CDS             1025..2029
                     /gene="plsX"
                     /locus_tag="VLL06_04210"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HSS30201.1"
                     /translation="MGSDHGPAPAIEGALQAAQECDVEVLLVGDESVLTAECRRLGCR
                     DSRVSIRHASQVVAMHESPATVARKKRDSSIWIATELVKSGEASAVVSPGNTGASMVA
                     SFFLLGLTKGVERPAIATSLPTLTGTAIMLDVGANVDCTAQHLAQFAIMGNEYGKYLF
                     GKPNPRVGLLSIGEEDSKGNEVTKEAFKLLKASSLNFIGNIEGRDVYSGGADVVVCDG
                     FIGNVALKISEGVADTIKKLLLKEISGSFFGRLAYPLIAKPLLNLKKRIDYAEFGGAP
                     LLGVNGITMICHGRSSAKAIKNAIRRAKGLAEHRVDELIQRDIEESLAQSKPTEDTQV
                     "
     gene            2026..3000
                     /locus_tag="VLL06_04215"
     CDS             2026..3000
                     /locus_tag="VLL06_04215"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HSS30202.1"
                     /translation="MRARIAGTGSYVPATVLTNADLERMVATSDEWIVERTGIRERHV
                     AGPGEACSDLAVKAAEQALAAAGVSAADLDLILLATCTGDYPLPATACLIQHQLGATR
                     AAACDVSAACCGFVYALSVADAYVKTGMRHVLIIGSEVMSAITDWTDRNTCILFGDGA
                     GAAVISASENDRGILSTHLRSDGALCELIAVPGGGSRMPPSEKVVAERMQYIKMKGNE
                     TFKVAVRTLEDIARETLAANHLCVEDLDLYVPHQANVRILKAVAERLGLPPEKVMLNL
                     DRYGNTSAASIPIALDEAVRQGRIQEGGLVMLGAFGAGLTWASALIRW"
     gene            3263..>4089
                     /gene="fabD"
                     /locus_tag="VLL06_04220"
     CDS             3263..>4089
                     /gene="fabD"
                     /locus_tag="VLL06_04220"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HSS30203.1"
                     /translation="MGRALVEAHPNIRSLYEEASSVLGYDIAALCFDGPAERLNLTEY
                     TQPALLVSSIAALHTFGPLGVLPIAVAGHSLGEYSALVAAGGLTYREAVAIVQKRGRY
                     MAEAVPSGTGLVAALLGLAVDVVKEVCREASSVGVVAAANFNSPGQIVIAGDKAAVER
                     AIELAKAKGCKKAIPLPVSVPVHTPLMQKAADRLAGDLAAITWSDLKIPLVNNAEARP
                     ITKAGEIQASLVRQLPSSVLWEDSVMAMAAMGVMTFVEVGPGTVLSGLIKRIVPDAVT
                     "
BASE COUNT          852 a         1030 c         1277 g          930 t
ORIGIN      
        1 ggaattgctc aggccgctgt tggctgacat cactgccgat gggctttcgc tggtcggaga
       61 agcgacagct gaagaacttg acctgaccga agatgatgcc gtcgtcgtcg gtccactggc
      121 tgtcagtctg gagttgacca atgtcgaagg gctggttgcc gtgaccggtg tattggaagg
      181 gatgattgtt cgcgaatgtg tacgatgtct caaagagtat gaggatccgc tggcgttttc
      241 tgtgcgggcg gcctttattc cggaacctaa atcggcgccg cgtcatccga agcgagtcga
      301 tttgagaaaa gcccatacca agatcgtgga ggccgaacaa gaagaggagc ccgacgatca
      361 gtaccaatat cagggtaatc agttggaact ggctccgatg ttgcgtgaac acgtgatcct
      421 atccgcgccg atgcagccac tgtgcaatga cgattgtttg gggctttgcg cacgatgtgg
      481 gaaaaacttg aatgaggggc cctgtcagtg tgccgaagaa cgaccgatcc cgacagttcg
      541 ggtcgtccag gtcacgaacc gcaaaactgg tggatcttca acaccataac gtgaccacgt
      601 gctatcaaaa gtgagaagga gttgccatgc cgaatccgaa acataaacat tcacgggcac
      661 gacgcgataa gcggcgcaca caaaaattgc gtatcactcc gccgggtatg tccgtctgtc
      721 cacagtgcca cgaaatcaag ctgccgcatt acacctgctt aaattgcgga acatataagg
      781 gcaaggcggt catccaggtc gaagagtcct agttaggttt gtggttgagc cgttgggctc
      841 caccaggccc catggcccaa tgaatcacaa tgggacggtg cttggttccc tgttgcgtca
      901 attctgtcac tgctctaaac tcctgccgca tgtcgtgtgg tgacggctgc tcgtctttcg
      961 tgaccacgtg gcacgtcatc attcgagaag tactcagccc gctatgaaga ttgcgcttga
     1021 tgccatgggc agtgaccatg gaccggcccc ggccatcgaa ggggcgctcc aggctgccca
     1081 ggaatgtgac gttgaggtgc tgctggtcgg tgacgagtcc gttcttaccg ccgaatgtcg
     1141 tcgtctcggg tgcagggact cccgcgtgtc tattcggcat gcctcgcagg tcgttgcgat
     1201 gcatgagtca ccggctaccg tagcccgcaa gaagcgggat tcctctatct ggatcgccac
     1261 ggagttggtc aagagcggtg aagcgagtgc cgtggtgagt cccggtaaca ccggtgccag
     1321 catggtggca tccttcttcc tgctcggctt gacgaagggt gtcgagcgtc cggcgatcgc
     1381 gacaagcctg cctactctga ccggtaccgc gatcatgctt gatgtcgggg cgaatgtgga
     1441 ctgtacggct caacacctgg ctcaattcgc cattatgggg aacgagtatg gcaagtatct
     1501 gtttgggaag cccaacccac gtgtcggatt gttgagtatt ggagaagaag acagcaaagg
     1561 gaatgaggtg acgaaggagg cattcaagct cctcaaagcg agttcgctca acttcatcgg
     1621 caatatcgag ggccgcgatg tgtacagcgg gggcgctgat gtggtggtct gcgacggctt
     1681 tatcgggaac gtcgcgctga agatctctga gggcgtggcc gatacgatta agaagttgct
     1741 actcaaggaa atctccgggt cgttcttcgg tcgcttggct tatcccctca ttgccaagcc
     1801 gcttctgaac ttgaagaaac gaattgatta tgcggaattc ggcggggcac cgcttctggg
     1861 cgtcaacggt attaccatga tctgccatgg tcgttcgtcc gccaaggcga tcaagaatgc
     1921 catccgccgc gcgaagggat tggcagagca tcgtgtcgat gaactgattc aacgcgatat
     1981 tgaagagagc ctcgcacaat ccaagcccac cgaggataca caggtatgag agctcgcatc
     2041 gcaggaaccg gatcgtatgt gccggctacg gtgttgacca atgccgatct cgaacgaatg
     2101 gtggccacgt cggatgaatg gatcgtcgaa cggaccggga tccgcgagcg ccatgtggcc
     2161 ggtcccggcg aagcctgttc tgacctggcg gtgaaggcgg cggaacaggc ccttgcggcc
     2221 gcgggggtca gtgcggccga tctcgacctg attcttttgg ccacctgcac cggtgattat
     2281 ccgcttcctg ccacggcctg tctgattcaa catcagttag gggcaacgcg tgcggcggcc
     2341 tgtgatgtgt cggctgcctg ttgtgggttc gtctatgcgt tatcggtggc ggatgcctat
     2401 gtgaaaaccg gcatgcgaca tgtgttgatc atcgggtcgg aggtgatgtc agcgattacc
     2461 gattggaccg atcgcaacac ctgtatcttg ttcggcgatg gggctggagc cgcggttatc
     2521 agcgcgagcg agaacgaccg gggcatcctg tccacacatc ttcgttccga tggcgccctc
     2581 tgcgaattga ttgcggttcc aggaggagga tcgcgcatgc cgccctctga aaaagtcgtg
     2641 gcggaacgga tgcagtacat caagatgaag gggaacgaga cctttaaggt ggcggttcga
     2701 acgttagaag acatcgcgcg cgagaccttg gctgcgaatc atctctgcgt cgaagacctc
     2761 gatctctatg tgccgcacca agcgaatgtg cgtattctca aggcggtggc tgagcgtctt
     2821 ggactcccgc ctgagaaagt gatgctgaat cttgatcggt atgggaatac ctcagccgcc
     2881 tcgattccta ttgccttgga tgaagcggta cgccaggggc gaattcaaga gggaggtctg
     2941 gtgatgttag gggcgttcgg cgctggattg acgtgggcat ctgctctcat tcgatggtga
     3001 tggggatggc tgaagcaggc ctcagacccc ccgccattcc ttgttccacc aaacaatttt
     3061 tcccgttgac attcaaggga gattcgacga tatctaaccc cacggagaca gcttgatacg
     3121 ccctctccag cttcgaatag tccaataatg cgcgtaacca gtagcctcgt acaatcgctc
     3181 cggtattatg gcaactctcc aatcagtaag gttctccaat gttgggtttg tcttccctgg
     3241 gcaggggtct cagtcagtcg ggatgggaag agcattggtg gaggcccatc cgaacattag
     3301 gagtctctac gaagaggcct cgtcggtgct aggatatgac attgctgcgc tgtgttttga
     3361 cggaccagcg gaacgattga accttacgga atatactcag ccggcattgt tggtgagtag
     3421 tatcgcggcg ctccatacct ttgggccatt gggtgtttta ccgatcgcgg ttgcgggtca
     3481 tagtctgggt gaatattcgg ctcttgtcgc cgcgggcgga ctgacctatc gtgaggcagt
     3541 ggcgattgtg cagaagcggg gtcgctatat ggctgaagcg gtgccatctg gaactgggct
     3601 tgtcgctgcg cttctcggtc tcgcggttga cgtggtgaaa gaggtatgtc gggaggcttc
     3661 ctctgtgggg gtcgtcgctg cggcgaactt taattcacct ggccagattg tcattgccgg
     3721 ggataaggct gccgtggaac gggcgattga gctggcaaag gccaagggat gtaagaaggc
     3781 gattcctctt cccgtgagtg tgccggttca tacgcctctg atgcagaagg cagccgatcg
     3841 actggccgga gatttagccg cgatcacctg gtccgatctg aagataccat tggtgaataa
     3901 tgcggaagcc aggccgatca ccaaggccgg tgagattcag gcgtcattgg tccgacaact
     3961 tccttcgtcg gtgctctggg aagattcggt aatggcgatg gctgcgatgg gtgtgatgac
     4021 atttgtcgag gtcgggccgg gaacggtgtt gagcggcttg attaaacgga ttgtgccgga
     4081 tgcagtgac
//
LOCUS       DATIPF010000161         4745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_649841, whole genome shotgun sequence.
ACCESSION   DATIPF010000161 DATIPF010000000
VERSION     DATIPF010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649841"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VLL06_04225"
     CDS             <1..695
                     /locus_tag="VLL06_04225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSS30204.1"
                     /translation="IVTGSSSGIGKAIALRFGAEGAKVVVTARRLALCEETVAQIKKK
                     GGEAWAIHTDVADERQVERLIDEAVKRYGRLDILVNNAGVVAGGRLAETTTKAFDEVM
                     NVNLRGTFFCCRAGFRQMKEQGGGIIINMSSVAGVQAWTGTGTYSASKHGIMALTKSL
                     ADEGRPHHIKVSAICPGGVADELIDASPEEIRRSEKIDPFDVAETALYLATLGKYSVV
                     HQIVIDRLGADW"
     gene            complement(968..2248)
                     /locus_tag="VLL06_04230"
     CDS             complement(968..2248)
                     /locus_tag="VLL06_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30205.1"
                     /translation="MSILRKRLAFSILFFLWLFVSTSMVSGTSATSTIYTYKDDSGTP
                     NYTTELDMIPEKYRSRIVPLDPETPSTDTPAEPYQPAANVRVVTASGEYQMGDHDTRA
                     DAVRLAVEAAKRQALEQVATYLESVTEVKNTDITRDDIRTYTAGIVMVLGQKISTRLD
                     GDTVIIHADLTAQVDPKEVAQAIETLRENESAKTELVSLRAETDQLHQQLDAANQALA
                     TATSPEQIQTLTQQRQDTLNQLQANALVSQAWTDWVYVTPIIYPYPWIGVQQVHGLLL
                     QARQLNPRNRHLQIVQQIVTAQTGALPPPSGTSSPVPPHTSLLVPPPASQRFVPSPFQ
                     RHQPGSGSPAPFMREGNSLSASPPQHFSNPLPPTLQQIHPPNFSQPNPPVLRAPFYAP
                     HSGSQMGPQHFGGSGGHSGGGGGRFGGSGGHRGR"
     gene            complement(2603..3055)
                     /locus_tag="VLL06_04235"
     CDS             complement(2603..3055)
                     /locus_tag="VLL06_04235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSS30206.1"
                     /translation="MTHIRPATESDFPALLQIQQDAFGEYASVYKVSGWTTETLDSLK
                     DDAKEKHIFVAEADGVVIGSVRFWNVAGVCVMRLLSVSPTHQSQGVAKLLIREIERVA
                     TDAHKFFACTMLNTARNIHLFMSLGYKAESILPDHYDHLDLICFAKYK"
     gene            complement(3176..3928)
                     /locus_tag="VLL06_04240"
     CDS             complement(3176..3928)
                     /locus_tag="VLL06_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HSS30207.1"
                     /translation="MMRKALAASPPMVRILVGAVLILLTWASVNWTYHAINKPSEVFF
                     PLDTSLAKSPTETWQQYGSLFRKHSTSVITSDLLAALAQVEGGGNPVARTYWRWSRTW
                     NPLELYRPASSAAGMYQITDSTFREAKRYCIHDHKVVEDGPWNDVNSCWFNSLYTRVA
                     PSHAIELTAAQLDRAVGSAIGQRKIATATLQRKQDLAAVIHLCGTGAGQAYARHGFRL
                     TAHQRCGNHDVNRYLAKVNGMKRQFARMASSS"
     gene            4238..4693
                     /locus_tag="VLL06_04245"
     CDS             4238..4693
                     /locus_tag="VLL06_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30208.1"
                     /translation="MAIRLIGCIVAVILVGSVEVQAQGYLDGLMDRATESAKRKAQER
                     VNQNVEQSIDKAIKKTEETVKCVATDRECLRRAKEEGKQVEMAEPPAPKDSIKCLVTD
                     TGCMKEAKTQRKKVEIVEESELDVLRCAATDADCLRRAQTLGKKVEIID"
BASE COUNT         1037 a         1233 c         1469 g         1006 t
ORIGIN      
        1 cgattgtgac tggcagcagc agcggcatcg gcaaagccat cgctctgcgg ttcggggccg
       61 aaggggcgaa ggtcgtcgtc acggcgagga ggctggcgct ctgcgaagag accgtagcgc
      121 agatcaagaa gaaaggcggg gaggcatggg cgattcacac cgacgtggcc gatgagcgcc
      181 aggtggaacg gctgattgat gaggcggtga agcgttatgg acgcctggat attcttgtga
      241 acaatgcggg agtcgttgct ggtgggcgat tagccgagac gacgacgaag gcattcgacg
      301 aagtgatgaa cgtgaacctg cgcggcacct ttttctgttg ccgggccggc ttcagacaga
      361 tgaaagaaca gggtggcggg atcatcatca acatgtcgag cgtcgccggc gtgcaggctt
      421 ggacaggaac cggaacgtac agcgcgtcaa agcacggcat catggcgctc acgaaatcgc
      481 tggccgatga aggccgtccg caccacataa aagtgagtgc catctgtccc ggcggcgtgg
      541 ccgacgaact catcgatgca tcgcctgagg agattcgccg gagcgagaag atcgatccat
      601 tcgacgtggc tgaaacggca ctgtatctgg ccaccctggg caagtattcc gtagtgcatc
      661 agatcgtcat tgatcggtta ggggctgatt ggtgaaggga tggagcctga tgatcttccc
      721 ttgctcgcgg aacgcgcacg atcagaatgt gctcgttcga cgcgcacaat cgaggatcga
      781 ccaggctacc cttgaaaatg aaatggggaa gtgggaagtc gcgcgcagtg gaagatcaat
      841 cagcccccat ccttgaagaa ataacgagca agcttggagg agcattgtat cgttggatgc
      901 gcgcggtgcg gggtccaacc acgctgcttt caatagagag taacgagcaa gctgttctcg
      961 agaatggcta acgaccgcga tgtcctccgc tcccaccgaa gcggcctccg cccccaccac
     1021 tatgccctcc cgatccgcca aagtgctgcg gacccatctg tgagcccgag tgaggcgcat
     1081 aaaacggtgc gcgaagcaca ggagggttcg gttgtgaaaa gttgggagga tggatttgct
     1141 gcaatgtcgg gggcagggga tttgagaaat gttggggagg cgatgctgat agagaattcc
     1201 cttcacgcat aaagggagca ggagaaccag atcccggctg gtgacgctga aatggtgaag
     1261 gaacaaaccg ctgcgaagcg ggcggaggaa ccagcaagga agtatgcggt ggtaccggcg
     1321 atgaggttcc tgacggcggc ggtagggcgc cggtctgagc cgtcacgatc tgttgcacga
     1381 tctgaagatg acgatttctt ggattgagct gtcgtgcttg gagtaacagt ccatggacct
     1441 gctgcacgcc gatccaggga taggggtaga tgatcggggt cacatagacc cagtcggtcc
     1501 aggcttggga gacgagggcg ttcgcttgca gctggttgag cgtgtcctgg cgttgctggg
     1561 tgagagtctg gatctgctcg ggcgacgtgg cggtggccaa tgcctgattg gcggcatcga
     1621 gctgctggtg taattggtcc gtctcggctc ggagcgacac cagctccgtt ttggcgctct
     1681 cgttttccct cagagtctcg atggcctgag cgacctcttt ggggtccacc tgtgcggtca
     1741 ggtcggcatg gataatcacc gtgtctccgt caagcctagt gctgatcttc tgacccaaca
     1801 ccatgacgat gccagcggta taggtccgga tgtcatccct cgtgatgtcc gtattcttca
     1861 cctcggtcac gctttccaga taggtcgcga cctgttcaag cgcctgtcgc tttgcagctt
     1921 cgacggccaa gcggaccgca tcggctctgg tgtcgtgatc tcccatttga tattcgccgc
     1981 ttgcggtcac gacacggacg ttcgctgccg gctggtatgg ttcggcagga gtatcagtcg
     2041 aaggtgtttc gggatcgagg gggacgatcc tactgcgata tttctccggg atcatgtcca
     2101 actctgtggt gtagttcggt gtaccggaat cgtctttgta ggtgtatatc gtcgatgtcg
     2161 cggaggtacc agataccata gaagtggaca caaagagcca caggaagaac agaatcgaga
     2221 acgcgagtct cttgcggagg atcgacatgg ggacccctac gatatcttgc tacctccagc
     2281 gtaccatata aatacctgtc cgctcgccgt gttcaggagt tcttcccgga aatcagaatc
     2341 ggtagacgag gactttttag ccgctggtga gtcaggtcta agctcggtct aatgcccggt
     2401 taatagtgac tggttacgct ctacttacac agaggagcgt gaccatgaca cacatcggca
     2461 tgcgggttta ttggacggcg atcggaatgt ttttctattc gctcctcgcc agtgcctctc
     2521 gagataaagc acggcgcaag gacacgtcag aactcaaccc ctgatgatcg gttggtccgc
     2581 gttttccagg caccagcgcg ggctatttat acttggcgaa gcagataaga tcgaggtggt
     2641 cgtagtggtc gggaagaatc gattccgcct tgtagccgag gctcatgaac aggtgaatgt
     2701 tccgggcggt gttcagcatg gtgcaggcaa agaatttatg agcatcagtc gcaacccgtt
     2761 cgatttcccg tatcagcaat tttgcgaccc cttggctctg atgggtgggg ctcacggata
     2821 acagtcgcat cacgcacaca ccagccacat tccagaaacg tacggatcca ataaccacgc
     2881 catccgcctc cgccacgaag atgtgcttct ccttcgcatc gtctttcagg ctatcgagcg
     2941 tttccgtcgt ccatccgcta actttgtaga cggaagcata ttccccgaag gcgtcctgtt
     3001 ggatctgcag taaggctggg aaatcagatt cggtggcggg gcggatgtga gtcatgaatg
     3061 gtccctcgtg gggatgagga tatcaaagcc cggccctatc ttctatcagt gacgtatcag
     3121 tgtctgttct gggggtcatc cgagagactg ttgctgaagg tggacgagtg gactgctagc
     3181 tggaggaggc cattcgggca aactgtcgtt tcatcccatt gaccttcgcg agataacggt
     3241 tcacgtcgtg gttgccgcag cgctggtggg cggtcagtcg gaagccgtgt cgtgcatagg
     3301 cctggcccgc cccggtgccg cacaaatgaa tcactgcggc caggtcctgt ttccgttgaa
     3361 gcgtagcagt ggcaatcttg cgttgtccga tcgcgctccc gacggcgcga tccaactgtg
     3421 cagccgtcaa ttcgatcgcg tggctcggcg cgacgcgtgt gtagaggctg ttgaaccaac
     3481 acgaatttac atcgttccaa ggaccgtctt cgaccacctt gtgatcgtgg atacaatagc
     3541 gtttggcttc gcggaacgtg gagtcggtga tctgatacat gccggccgcg ctcgacgcag
     3601 gccggtacag ttccagagga ttccacgtcc tgctccaccg ccagtatgtc cgcgccaccg
     3661 ggttgcctcc gccttcgacc tgagcgagcg cggcgaggag atcggacgtg atgacggagg
     3721 tcgaatgttt gcggaagagc gatccgtact gttgccacgt ctcagtcggg ctcttggcga
     3781 gcgatgtgtc cagcggaaag aacacctcag acggcttgtt gatcgcgtga taggtccagt
     3841 tcacgctagc ccacgtcaac agaatgagga ctgcaccgac gagaatacgg accatcggcg
     3901 gcgaagccgc caatgccttc ctcatcatct ggaactcacg ccatctcgac cgtgcgagac
     3961 gcctgattgc ggcaagtctg aaccgacggc tgcgccttct ccggggagca gctctgttcg
     4021 ctcgcgcata ggatgcggaa gccatggtcc actattataa ccgctagagg gaaacgagca
     4081 aggttggaac ctcagcttat tttatgccga agacttttcc ctgaacccct ttaaggtaaa
     4141 caggttagag ggtttcccag ggtctgctat tctttaatgg tagatcagtg gatttcctct
     4201 cagcagcttc cacctctcgt ggcgaagggg gttcatcatg gcgatacgcc tcatcggctg
     4261 catcgtagcg gtcattctag taggtagtgt agaggttcag gctcaggggt acctcgatgg
     4321 cctcatggat cgagccaccg aaagcgccaa acgcaaagcg caggagcgcg tgaaccagaa
     4381 cgtcgaacaa tcaatcgaca aggccatcaa gaagactgaa gagacggtca agtgtgttgc
     4441 gacggatcgg gaatgcctca ggcgcgcaaa ggaggaaggg aagcaggtcg aaatggctga
     4501 accgccggcc cccaaagaca gcataaaatg tctggtgacg gacaccggct gtatgaagga
     4561 ggcgaagacc caacgtaaga aggtcgaaat tgttgaagaa agtgaactcg acgtgcttcg
     4621 ttgtgcagca acggatgccg actgcctcag gcgtgcacaa acgctgggga agaaggtcga
     4681 gattatcgac tgagcaaagg tggacactcc gatatcccct gactccgcga gtcacgcttg
     4741 ccaac
//
LOCUS       DATIPF010000162         2232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_30651, whole genome shotgun sequence.
ACCESSION   DATIPF010000162 DATIPF010000000
VERSION     DATIPF010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30651"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VLL06_04250"
     CDS             complement(<1..682)
                     /locus_tag="VLL06_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HSS30209.1"
                     /translation="MPELPEAEVVARQLRVHLLGAQLNECLVGRADIVREGLATLSWY
                     YGAVLEDVERYGKSVVLGLRKENETRYVVAELGMTGLLLFRATQTKYPQHVHVRMSFT
                     GGHESELRYWNPRRFGRISLLDRAGLARYRARRFGIDPLTVPRDEFIRLMEVRRGRLK
                     PLLMHQQVIAGIGNIYANEILFRARLHPNREVHRLRRATIMTLYDTMQTVLRESISCG
                     GSSVKDCYA"
     gene            complement(815..1270)
                     /locus_tag="VLL06_04255"
     CDS             complement(815..1270)
                     /locus_tag="VLL06_04255"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HSS30210.1"
                     /translation="MHLINETLLVGNINDARDPPVKIGALLLLAGEYTLEPPDGLDYG
                     RIPFLEFGEAKPLLLDRAVSWVERHMSERRVMVCCRAGMGRSVSVAMAYLCCVEGMTY
                     AEVLKLVLKRRPGAMPLPKLEEAIIEVCLLREARVTDKKTGASRPATAV"
     gene            1403..1762
                     /locus_tag="VLL06_04260"
     CDS             1403..1762
                     /locus_tag="VLL06_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30211.1"
                     /translation="MPSITVKEVEARLESVHCAICKGSSFGIDQRFSQSDGEWRGVCK
                     KCYYSFPVYTDMEFYLRTQPDIPFRLKEMSCPTCNLKGVSLDFRITMSVRESIYFLTC
                     SNCKRTFPQQSSLEAFE"
     gene            1801..1995
                     /locus_tag="VLL06_04265"
     CDS             1801..1995
                     /locus_tag="VLL06_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30212.1"
                     /translation="MSDTPKSRAKGPESPAPESVPRVRKEIPLVSVPNDRDMIAEEMA
                     EMFDEDRVSHQHGSSEPEAD"
     gene            complement(2002..>2232)
                     /locus_tag="VLL06_04270"
     CDS             complement(2002..>2232)
                     /locus_tag="VLL06_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30213.1"
                     /translation="VVSELRELKGDTRAWLCNELGRSEVGRLDRAESETNAAWDHCRR
                     GTIVRIEGLKRKEGATLERIPTEGTVEMIRPA"
BASE COUNT          524 a          662 c          557 g          489 t
ORIGIN      
        1 gtgcatagca gtccttcaca ctcgacccgc cacaagaaat cgactcgcga agcaccgttt
       61 gcatggtgtc atagagcgtc ataatcgtag ccctgcgcag ccgatgcacc tcacggttcg
      121 gatggagcct ggcgcgaaaa agaatttcgt tggcataaat attcccaatc cctgcgatca
      181 cttgctgatg cattaacagc ggcttcagtc gcccacgcct gacttccatc agcctaataa
      241 actcatcgcg cggcaccgtg aggggatcga tcccaaaacg gcgagcacgg tagcgtgcaa
      301 ggcctgctcg atcgagcaag gaaatccgcc cgaatcgtcg cgggttccaa tagcgtagtt
      361 ctgattcatg cccccccgtg aacgacatgc gaacatgcac atgctgagga tatttcgtct
      421 gcgtcgcgcg aaagagtagc aggcccgtca tgccaagctc tgcgacgaca tatctggtct
      481 cattctcttt tctcaaccct agtacgacgc ttttgccgta tcgctctaca tcttccagca
      541 ccgcaccgta ataccacgag agcgtggcca atccttcccg aacgatatct gctcgtccaa
      601 ccagacactc attaagctgg gcacctagta ggtggacccg taattgccgg gcaacaactt
      661 cggcttctgg caattccggc atcgggagag gtccttcgtg aaacaggtgt atatatgcgt
      721 gattgtggat taggccatag gaagcagggg gaaggcaatg caggcggaca cgcgtaggat
      781 aacgacggag gcagatacct ctgtacggcg atgattacac ggctgtggcg ggacgtgacg
      841 caccggtctt cttgtccgtc acacgagcct cgcgaagaag gcacacctcg atgatggctt
      901 cttcaagctt gggaaggggc atggcacctg gtcgccgttt caagaccaat ttcaagacct
      961 cggcatacgt cattccttcg acacaacaga gatacgccat ggccacggac acggaccggc
     1021 ccataccggc acggcaacag accatgactc ggcgttccga catatggcgt tcgacccaac
     1081 tgacggcccg gtccaacaag agcggcttgg cttcaccaaa ctctaaaaat ggaatcctgc
     1141 catagtccaa cccatcgggg ggctccaacg tatactcccc ggcaaggagg agcagggcgc
     1201 caatcttgac cggcggatcc ctcgcatcgt tgatattccc gaccaaaagc gtctcgttga
     1261 tcaagtgcat gacggtctca gtataagcag ctcgatcgcc aggtcaaggg gaaaacttgc
     1321 ctccgtccgg ttgcggtcgc ttccatgtga acgctatact caccgcacaa tgtcgccaac
     1381 ttctgcacaa ggagcctccc ccatgcccag cataaccgtg aaagaagttg aggccagact
     1441 tgaatctgtt cattgcgcca tttgcaaggg gtcaagcttt gggattgatc aacgtttttc
     1501 gcagtctgat ggtgaatggc gtggcgtctg caaaaaatgc tactacagct ttcccgtcta
     1561 cacagatatg gaattttacc tgcgcacaca acccgatatc cccttccggc tcaaggagat
     1621 gtcctgtccg acctgtaatc tcaaaggggt gagcctcgat tttcgcatca ccatgtccgt
     1681 ccgagaatcg atctatttcc tgacatgtag caactgcaag cggaccttcc ctcagcagtc
     1741 gtcgctggaa gcattcgaat aactcacctt ttgaccattg ccgtagtgta tactttcatt
     1801 atgagtgaca caccaaagag tcgcgccaag ggccccgaaa gtcccgctcc cgagagcgtg
     1861 cccagggtaa ggaaagaaat cccactcgtg tcagttccca acgatcgtga catgattgct
     1921 gaagaaatgg cggagatgtt cgatgaagac cgagtctcgc atcagcatgg gagttcagaa
     1981 ccggaagccg attagccaag cttaggccgg tcgtatcatc tcgacagttc cttctgtggg
     2041 tattctttcc aacgttgccc cttccttccg cttcaatccc tcgatcctga cgatcgttcc
     2101 ccgtcggcaa tgatcccacg ctgcattggt ttcggactct gcccgatcga gtcgcccaac
     2161 ctccgaccgg cctaactcat tgcagagcca tgctcgcgta tcgcccttca attcacgtag
     2221 ttcgctcacg ac
//
LOCUS       DATIPF010000163         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_118781, whole genome shotgun sequence.
ACCESSION   DATIPF010000163 DATIPF010000000
VERSION     DATIPF010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118781"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VLL06_04275"
     CDS             complement(<1..334)
                     /locus_tag="VLL06_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30214.1"
                     /translation="MAFTLLEIVEQQARSNEIDAALDTARRIPNAADWSAALEAIAIG
                     QFKAGDQRGALHTAKLIPHEINRTGALMHIAFEQARSGHVDRAIETTTHIKAPLRRDA
                     VLLGVALIQ"
     gene            complement(479..1114)
                     /locus_tag="VLL06_04280"
     CDS             complement(479..1114)
                     /locus_tag="VLL06_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSS30215.1"
                     /translation="MPKPRVLLADDHVLVLEGFRRILQEHYELVGTVGDGYALLSAAK
                     TVQPDIVILDISMPLLNGIETATQLKKICPAAKIIIVTMHAGADYVRSAFEAGASAYV
                     LKGSAVDELEQAIRAVLGGLSYITPLITKELVDVYLSTPSEKPRGLTPRQREVLQFLA
                     EGRTAKEIANLLHITSRTVEFHKGQILDQLKLKTTADLIKYALTHGIVNTP"
     gene            complement(1107..2297)
                     /locus_tag="VLL06_04285"
     CDS             complement(1107..2297)
                     /locus_tag="VLL06_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HSS30216.1"
                     /translation="MYRKQDAKSHNTTGRLLILIIGLGIGVFFLDLSLPLGIGNSVLY
                     GGLVILSFLLPYRKGPLITAIICSVVDMTDILLKPTLDGVPLWMGVTNRLFSLTAIWL
                     PLLFFLHRRRAEDLLQRAHDELETRVEARTHELATVNQALVTEIKEHTETEQSLRTSD
                     AVLRASQHELQQSRADLRALAGQLLTAQEADRRRISRDLHDDINQRLAMLSMELRRME
                     KDPSNDPEPFRDGIRRVSDGLAAVSDDVRQMAYRFHPSILDDLGLLKAVRRLANEFSE
                     RTGIPCSYVHKDPMGILPPDVTICIYRVVQECLSNISRHAQATQVEVELICEDDEVSL
                     SVRDNGVGFDASHGDQMSGHLGLLSMNERVRMAKGTLEVESTPQHGTEIRVDIPLGPG
                     EPHA"
BASE COUNT          567 a          731 c          720 g          591 t
ORIGIN      
        1 cttggatcaa tgcgaccccg agcagtaccg catctcttcg taaaggcgct ttgatgtgag
       61 tggttgtctc aatcgcgcgg tctacgtgtc ccgatcgtgc ctgttcgaat gcaatgtgca
      121 tcaaggcacc ggtacggttg atttcatggg gaatcagttt ggccgtgtgg agagcgccac
      181 gctggtctcc ggccttgaat tgaccgattg cgatggcttc taaagcggct gaccagtctg
      241 ccgcattagg aattcttcta gccgtgtcga gcgccgcatc gatctcattc gatcgcgcct
      301 gctgctcgac aatctccagt aacgtgaacg ccacctcagc accatatgcg aagaggacga
      361 actgtgttta ggaattcttc acacacaaat ctacccctct cgctcatgga atggaactcg
      421 tagaaaccgc aggtatacgg gagaatttct acgaggggga gaggggctga tcggtcgatt
      481 agggtgtgtt gacgatgccg tgggtcagtg cgtatttgat caaatctgcc gtcgtcttca
      541 gcttaagttg gtccaggatc tgtcccttat gaaactcgac ggttcgtgat gtgatatgca
      601 agagattggc aatctccttg gccgtccgcc cctcggcgag gaactgcaag acttcacgct
      661 ggcgaggagt aaggcctctc ggtttctccg aaggtgtgga caaatagacg tctaccagct
      721 ctttcgtaat cagcggcgta atgtaggaaa ggcctccgag cacggctcga atcgcctgtt
      781 ccaattcatc aactgccgac cccttgagca cataggccga ggctcctgcc tcgaaggccg
      841 atcgcacata atccgcaccg gcgtgcatcg tcacgataat aattttggct gctggacaaa
      901 tctttttcag ctgcgtcgcg gtctcgatgc cgttgagcaa cggcatcgag atatccagga
      961 tgacaatgtc gggctggacg gtcttcgcag cagacagaag cgcgtaccca tccccgactg
     1021 tcccgaccag ctcatagtgc tcttgcagga tacggcggaa tccctcgagg acaagaacgt
     1081 gatcgtctgc cagcaggacg cgtggtttag gcatggggct ctcctggacc caatggaata
     1141 tccacacgga tctctgtgcc gtgctggggt gttgactcca cctccaacgt tccctttgcc
     1201 atgcgtacac gctcgttcat gctcagcaag cccaaatgtc cgctcatctg gtctccgtgt
     1261 gaggcgtcaa atcccacgcc gttgtcacgg actgaaaggc tgacctcatc gtcctcgcaa
     1321 atcagctcga cctccacctg tgtggcctgg gcatgccggg agatgttgct aaggcattcc
     1381 tgtacgacac gatagataca aatggtcacg tcgggtggca gtatgcccat cggatcttta
     1441 tgtacgtagg agcacgggat gcctgtgcgc tcggaaaact cattggccag gcggcgtacg
     1501 gccttcaaga gccccaagtc atcgagaatg gacggatgaa agcgataggc catttgtcgc
     1561 acatcgtccg agacagcggc caggccgtcg gatacccgtc gaatcccatc ccggaaaggc
     1621 tccggatcat tcgacggatc tttctccatg cgtcgaagtt ccatcgagag catcgcgagg
     1681 cgttgattga tgtcgtcgtg caggtcgcgg gatattctcc gccgatctgc ctcctgtgcc
     1741 gttaatagct ggcctgccaa ggcccgcaaa tccgcacggc tctgctggag ttcgtgctga
     1801 ctggcccgca acacggcgtc gctcgtccgg agcgactgct cggtttccgt atgttctttg
     1861 atctccgtga cgagcgcttg attcacagtg gcgagttcat gtgtgcgagc ctccacccgc
     1921 gtttcgagtt catcgtgcgc ccgctgtaac aggtcttcgg cccgtcgccg atgcaagaaa
     1981 aacagcaagg gcagccaaat cgccgtcagg ctgaacaacc gattggtgac gcccatccac
     2041 agtggcacgc catccagcgt gggcttcagc aggatatcgg tcatatcgac cacagagcaa
     2101 atgatcgccg tgatcagtgg gcctttccga tatggcaaga gaaaggacag aataacgagt
     2161 ccaccgtaga gcacggaatt cccgattccc agaggaagag agagatcaag gaagaagacg
     2221 ccaatgccca aaccgatgat aaggatcagc agccgtccgg ttgtattgtg actttttgcg
     2281 tcctgtttac gatacatggg ttgcgccgat ttacctcgag cggattgagg actcgacggc
     2341 tcaagttgga gttccgaata agttcttccg tgactggtta cgccgacacc atagaagatc
     2401 tccctcatct ctgtccatta tccatccgcg aaggaaccgg cagcaagagc ttgcactctg
     2461 ccggtgacag gcaatttgga cggtctgctt cgatcgggac aaacggtcac aggtcgactg
     2521 agatgaacag ccgatatcac attcgaacct gtccacgttg atgcctcggt gacgcatctg
     2581 cactgcctcg tagtcatatg catacctcg
//
LOCUS       DATIPF010000164         3615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1708346, whole genome shotgun sequence.
ACCESSION   DATIPF010000164 DATIPF010000000
VERSION     DATIPF010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708346"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1974)
                     /locus_tag="VLL06_04290"
     CDS             complement(<1..1974)
                     /locus_tag="VLL06_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="marine proteobacterial sortase target protein"
                     /protein_id="HSS30217.1"
                     /translation="MLYVHLRSSVVRLLLCILITAFPLLIHPTARLAAQLAEPLSTGI
                     VSTMSINEITKGTLLFRTNQPGRFTPAPILKTDVQIAITGIIARTTVRQEFTNPSEKK
                     GDWLEGIYVFPLPETAAVDHLRMKVGERIIEGQIKERGEAKKVYEQAKQAGKRASLME
                     QERPNIFTTSVANIGPREHITVEIEYQETVRYDQGQFQLRFPMAVGQRYIPGTPVIVE
                     DQEPNGTGTLLDTDRVPDASRITPPGQHHSQGSIHGSGNPVSLSLTLTPGFPVAKVES
                     LFHPVIAIPDPDGGYQISLRKDAVPADRDFQLTWVPAPSQEPNATVLTEQRNDETYAM
                     LMLMPPTHSNDAAPRMPRDLTFVIDTSGSMAGPSIEQAKSSLTAALTRLTTQDRFNVI
                     QFNNTVRSLFSTPQPVTTAAMRKAIRYVEGLSADGGTDTLPALRQALKSQQDSSRLQQ
                     IILITDGQIGNEDELFELLTHRLGTRRVFTVGIGATPNSYLMRKTAEFGRGTFTYVGN
                     VSEVKEKLDGLFKKLEHPVLNDIQLDRTGWTGLEHYPSLIADLYEGEPIVLAVKAASL
                     PDQAVLRGQIGTQPWSLPLSMKQATVRGGLSIYWARQKISALMDEAFTGTAEETVRKG
                     VLDVALAHHLVSKYTSLVAVDVTPARPTGTSEPG"
     gene            complement(2076..2891)
                     /locus_tag="VLL06_04295"
     CDS             complement(2076..2891)
                     /locus_tag="VLL06_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSS30218.1"
                     /translation="MPNPSSPVSSGNISPAIVGPSKDTKEVYLIGGLVMAVAIVTGGF
                     WYYSGQDQPYASKTQSEPLTNAQVAHALKTSMIPPVEPIVHSQTVPAANYKSDSIHTD
                     LYFEVGRKGLTDDARAILGTQADLAKNDPDLGILVQGYTDRQGSTGYNLKLGMKRAET
                     VKAELMNAGVMEHQIKVVSLGKDGALCLDNSDVCRNMNRRVHLEIRKIGKDHMLLPTV
                     AAESATIEPMQSANDQTTNTDGHGSFEGDLLPSLADPSATDPSPLTTDPASGS"
BASE COUNT          722 a          897 c         1148 g          848 t
ORIGIN      
        1 tcctggctcg gacgttccgg ttggtctggc cggggtgacg tcgacggcga ccagactggt
       61 gtacttactg acgagatggt gcgccaaggc gacgtcgagt actcccttgc gaacggtctc
      121 ttcagcagtg cctgtgaagg cctcgtccat cagggcggag atcttttgcc ttgcccaata
      181 gatggagagc ccaccacgaa ccgtggcttg tttcatcgac aggggtagcg accatggctg
      241 agtgccgatc tgtcctcgga gcacggcttg gtccgggagg gacgccgctt tcactgccag
      301 gacaatcggt tccccttcat agagatcggc aatgagtgag ggatagtgct ccaggccggt
      361 ccatccggtc cgatcgagct gaatatcgtt cagcaccgga tgctccaact tcttgaacag
      421 gccatcgagc ttctctttca cctcgctcac gttccccaca taggtgaagg tgccacgacc
      481 gaactctgcg gttttgcgca tcaggtaact attaggtgtg gcgccgatcc caactgtgaa
      541 gactcgcctg gttcccaggc gatgggtgag tagctcaaag agttcatctt cgttgccgat
      601 ctggccatcg gtaatcagga tgatttgctg gagtctagag gagtcctgct gactcttgag
      661 ggcttgtctg agtgccggga gcgtgtccgt ccctccatcg gcggacaggc cttccacgta
      721 tcgaatggct tttctcatcg ccgctgtcgt gactggttgt ggagtggaga agagggaacg
      781 gactgtattg ttgaattgga tcacgttgaa acggtcttgg gtcgtcagcc gtgtgagggc
      841 cgctgtgaga gaagatttag cctgctcgat cgaggggccg gccatcgatc cggacgtatc
      901 gatgacgaag gtgagatcgc gaggcatacg cggcgcggcg tcattggaat gcgtaggagg
      961 cattaacatc agcatcgcat aggtttcatc gtttcgttgt tcggtcaaga ctgtggcatt
     1021 cggttcttgg ctcggggctg gaacccaagt gagctgaaag tcacgatcgg ccggcacggc
     1081 atcttttctg agactgattt ggtagcctcc gtcaggatcg gggatggcga tgacaggatg
     1141 gaaaagcgat tccaccttgg cgacgggaaa tccaggggtg agggtcaagg agagactgac
     1201 aggattgccg gatccatgaa tggacccttg gctgtgatgt tgccccggtg gagtaatccg
     1261 tgaggcgtcc ggtacccggt ccgtatccag cagcgtgcct gtaccgttcg gctcttggtc
     1321 ttccacaatg accggtgtgc cgggtatgta gcgctgtccg acggccatgg gaaagcggag
     1381 ttggaactgg ccctggtcat agcggacggt ttcctggtat tcgatctcaa ccgtgatgtg
     1441 ctcccttgga ccgatgttgg cgaccgaagt ggtaaagatg ttcggacgtt cctgttccat
     1501 cagactcgca cgtttccctg cctgtttggc ctgttcatag actttttttg cttcgccccg
     1561 ttccttgatc tggccttcga tgatccgttc acccaccttc atacgcaagt gatccactgc
     1621 ggcagtttcc gggagcggga acacataaat cccttctagc cagtcgcctt tcttctcgct
     1681 gggattcgtg aattcttgac ggactgtggt acgggcgatg atgccggtaa tggcaatctg
     1741 cacgtctgtt ttcaagatgg gggctggggt aaatcggccc ggctggttgg ttcgaaagag
     1801 cagggtgcct ttcgtgattt cgttgatgct catcgttgaa acaatgccgg tgctgagcgg
     1861 ttcagccagc tgtgcggcca gcctggctgt gggatggatc aggagaggaa aggcagtgat
     1921 cagaatacag agaagcagac gaacgactga agagcgcaga tggacgtaca gcatggtgaa
     1981 cctcggtgat gtggatggta aaagagtgaa tgactctgtc ggtaaccgga tcctggagga
     2041 actggtggcc cctccaggat cagattcggt gccggttagc tgccggaagc cgggtctgtc
     2101 gtcagtggag acggatcggt tgccgaggga tcggcaaggg aaggaagcaa atctccttcg
     2161 aatgagccgt gaccatcggt gttggtggtc tggtcattgg cagattgcat cggctcaatg
     2221 gttgccgatt ctgcagcgac ggtgggaagg agcatgtggt ccttgccgat ctttcggatt
     2281 tcgaggtgca cccgccggtt catgttgcgg cagacgtcgc tgttgtcgag acagagtgcg
     2341 ccgtccttgc cgaggctcac gaccttgatc tgatgttcca tgacaccggc gttcatcaat
     2401 tcagctttca cggtctcggc ccgcttcatg cccagtttca gattgtagcc ggtcgatccc
     2461 tgccggtcgg tatagccctg cacgagaatg ccgaggtctg gatcattctt ggccaagtcg
     2521 gcctgcgtcc cgaggatcgc cctggcatcg tctgtgagcc ccttacggcc cacctcgaaa
     2581 tagagatccg tatggatgct gtcggacttg tagttggcgg cggggacggt ctggctgtgc
     2641 acgatcggct cgacaggagg gatcatactg gtcttgagcg catgcgcgac ctgcgcgttg
     2701 gtcaggggtt cagactgggt tttcgaggca tagggctggt cctgtcctga gtagtaccag
     2761 aaaccgccgg tgacgatggc aacggccatg accagcccgc ctataagata gacctctttc
     2821 gtgtccttgc ttggcccgac gatggcaggg gaaatatttc ctgacgacac tggcgatgat
     2881 ggattcggca tgatggcgat tctcctattt tcatgaaacg tgaaaaatac aaagtgaaat
     2941 ggttgtgaaa actgcttcgg ccacgctcct cagtcacctc ctttccggcc gttggtctct
     3001 tgcgccgatg gctagatgct tgagcaaccg gggtgccgat aaaggagaac aaaggaattg
     3061 aaataactgt gaggaaaatt caggggttca gagagtgggg gagacgtacg cttgatgcgg
     3121 ggagagatga accggcatgg gatgcgggga tggccccgca agaagggagg ggggatggag
     3181 cctgatgatc ttctgtgctc gcgcaacgcg cggcctgaga aggccctcgt tggacgcgcg
     3241 caatagagga tcgaccaggc cgccccctaa agagctaatg gcacgtctcg gcgtgccagt
     3301 gggtgggcgg gtgagcgggt gagaaagttg cgcgcagtta gatcaaccag gctactcccc
     3361 ctgcgatggg ttgaaacgac ctatgggtat ggataagcaa aagcagttgc agtagactcg
     3421 cccgcaatag atcgtgcctc tcatctcaac tgttcggggt gtgaatgaag aaaaaagtag
     3481 aagtagcagt agcagtagca gtagagccgt cccttgtaga caaactgccc atttatatgg
     3541 tggcctggtc attgccaccg gcttttgtga tcctggcagt ggttgtctca gcgttggatt
     3601 ctttcaatga ggctg
//
LOCUS       DATIPF010000165         2874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_297480, whole genome shotgun sequence.
ACCESSION   DATIPF010000165 DATIPF010000000
VERSION     DATIPF010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297480"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VLL06_04300"
     CDS             <1..512
                     /locus_tag="VLL06_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HSS30219.1"
                     /translation="RVTRSVATRLFKRLQLVSACFMAFSHGANDAQKAMGIITLALLA
                     AGQIPSNEVPGWVIGACAVAMGLGTAAGGWRIVRTLGMGIVKLEPVHGFAAETGAAAV
                     LLVTAYVGLPVSTTQTITTSVMGVGAIKRLSAVRWGVTARILYAWIFTLPGAALLASL
                     IYLIGTRGV"
     gene            complement(659..2485)
                     /locus_tag="VLL06_04305"
     CDS             complement(659..2485)
                     /locus_tag="VLL06_04305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS30220.1"
                     /translation="MTLSIRWKVTIGTLLVLVCGLLIARTLAIRSLEQQEIVQSGQIL
                     EARTSLVAYGLQPFLTPSGALSSTSQLRAAVHDLSTRALARVTVIAPDGQVLADSAVS
                     DSNLPAVENHLARPEIQQAIATGRGTDLRTSHTTGERTLYLAIGLNSANQATPSGFLR
                     LGLPMTTFDREVDKLHQNLALAFGIAFVIALALSVWLARSITQPLSNIAIAAQQLAKG
                     DHAVRIRTGSRDEVGLLADTLNHMTDQLKSKIDELSEDRAQLLAMLTSMVEGVMVLDC
                     RGRVLQVNPALERMFDVTRIEARGHPCSDVFRHPQLDTLVSTVLTKRLNEEDEILLHP
                     NGRCLHIEASVTACDRENEACAVLVFHDVTELRRLENIRKDFVANVSHELRTPLTSIK
                     GYIEALLDGAKDDPDISAKFLDIILKQSDRLNLILEDLLQLSKIESGQVLFKREPLHI
                     RRVIERTLAMIKPLADKKGHRLASFVEDDLPTVLGDEDRLTQVMSNLLDNAVKYTPER
                     GTITVAAHPVSDDAEQPALIMAVELSVTDTGIGIPEQDRPRVFERFYRVDKARSRELG
                     GTGLGLAIVRHIVEGQGGRVWVEANTPTGSRFVVRLPVPQIR"
     gene            complement(2482..>2874)
                     /locus_tag="VLL06_04310"
     CDS             complement(2482..>2874)
                     /locus_tag="VLL06_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSS30221.1"
                     /translation="VTKPFSPKTLVARIKALFRRLQRKPEDSPAHYRYGDLVMDLARH
                     EVTVKNKEVPLTAKEFGLLEHLLRNPGRVLTRDILLNTVWGYDYYGTTRTVDVHIRRL
                     KQKLPSLNEAIMSVKSLGYKLKELDRSA"
BASE COUNT          565 a          766 c          882 g          661 t
ORIGIN      
        1 ttcgtgtgac ccggagcgtg gccactcggc tgttcaaacg gctgcagctg gtatccgcct
       61 gtttcatggc gttcagccac ggcgccaacg atgcgcagaa ggccatgggc atcattacat
      121 tggcgttgct cgctgcgggg cagattcctt cgaacgaggt gccgggttgg gtaattggcg
      181 cctgtgcggt ggcgatggga ttgggaacag ccgcgggcgg ctggcggatc gtccgtacgc
      241 tgggaatggg catcgtcaag ttggagccgg tgcatgggtt tgccgcggag accggcgcgg
      301 cggcggtgct gctcgtgacg gcgtatgttg gtctgccggt gagcacgact cagacgatca
      361 cgacgtcggt catgggggtc ggcgcgatca agcggctttc ggcggtacgc tggggcgtca
      421 ccgcaagaat tctgtatgcc tggattttca cccttcctgg agccgctctt ctcgcatcgc
      481 tcatttatct tatcgggacg agaggggtgt agcggctgag aacacactca ttaggactgt
      541 ctagtttctt cggtgtatcc gtttgccctg accgagccaa atagacgaga cagacgaaat
      601 agaacagatg aacaagatga gctgatggag tttccccagc atcctgtcaa tcagctgatt
      661 aacggatctg tgggacaggg agccggacaa caaatcggct tcctgtcggg gtgttggctt
      721 cgacccacac ccgcccgcct tgcccctcga cgatatgcct gacaattgcg agcccgagcc
      781 ctgttccacc caattcacgc gagcgtgcct tatcgacacg ataaaatcgt tcgaatacgc
      841 gcgggcggtc ctgctcaggg attccgatgc cggtatcggt gacgctcaat tccacagcca
      901 tgatcaacgc gggctgttcg gcatcgtccg aaaccgggtg ggccgcaaca gtgatggtcc
      961 ctctttcggg ggtatatttg acggcgttat cgagcaagtt ggacatcacc tgtgtcaacc
     1021 ggtcctcatc gccgagtacg gtgggcagat cgtcttctac gaaggaggcg agccggtgcc
     1081 ctttcttgtc tgctaagggt ttgatcatcg ccagcgttcg ttcgatgact ctccggatat
     1141 gaagcggttc tcgtttgaac aggacctgcc cggattcgat ctttgacagc tggaggaggt
     1201 cttccagaat cagattcagc cggtcgcttt gcttcaggat gatgtcgagg aattttgcac
     1261 tgatgtcagg atcgtctttg gcgccatcga gcaaggcttc gatatacccc ttgatggatg
     1321 tgagtggagt tctcagttcg tgggacacat tggcgacgaa gtcctttcgg atattttcca
     1381 ggcggcgtaa ctcggttacg tcatggaaga caaggaccgc gcaggcttcg ttctctcggt
     1441 cgcatgccgt gaccgaggcc tcgatatgaa gacagcggcc attcggatgt agaagaattt
     1501 catcttcttc gttcagacgt ttcgtcagga ctgttgagac caacgtatcc agctgcggat
     1561 gtcggaagac atcggagcag gggtgtcccc gtgcttcgat cctggtgacg tcgaacatgc
     1621 gttccaaggc tggattgact tgcaacacgc ggcctcggca gtccagcacc atcacgcctt
     1681 cgaccatcga ggtcaacatg gccaagagtt gggctcgatc ttcggagagc tcatcgatct
     1741 tggatttgag ttggtccgtc atgtgattga gcgtgtcggc caaaaggccg acttcatccc
     1801 gtgatccggt ccgaatgcgg accgcatggt cacccttcgc cagctgttgc gctgcgatcg
     1861 caatattgga caggggttgt gtgatgctgc gagcgagcca cacgctcagc gcgagagcaa
     1921 tcacaaatgc aatgccgaag gccagggcca ggttctgatg caacttgtcc acttcacgat
     1981 caaaggtggt catcggcaac cctagcctca agaaaccgga tggcgtggcc tggtttgcac
     2041 tgttcagacc gatagccaaa tacaacgtgc gctcaccggt ggtgtgactt gtgcggagat
     2101 ccgttccgcg cccggtggcg atggcctgct gtatttctgg tcgcgccagg tggttctcaa
     2161 ccgcagggag gttgctatcc gagacggcgc tatcggccaa cacctgtccg tccggagcaa
     2221 tgacggtcac gcgtgccagg gcccgcgtgc tcagatcgtg aacggctgcc cgcaattgtg
     2281 aggtcgagga tagcgctcct gatggggtca gaaacggctg cagcccgtaa gcgacgagac
     2341 tggttcgggc ctccagtatc tgccccgatt gaacgatctc ctgttgttca agggaacgaa
     2401 tcgccaacgt acgggcgatg agaagcccac agacgaggac tagcaaggtt ccgatcgtga
     2461 ccttccatcg gatggagagg gtcatgccga acgatccagc tctttgagct tataccccag
     2521 agatttgacc gacataatcg cctcattgag cgaggggagt ttctgcttga gtctacggat
     2581 atggacgtcg actgtccggg tcgttccgta gtaatcgtat ccccagacgg tattgagcag
     2641 gatgtctcgt gtcaggactc tcccggggtt gcgcaagaga tgttcgagta ggccgaattc
     2701 tttcgctgtg agggggactt ctttattctt caccgtgact tcatgtcgtg cgaggtccat
     2761 gacgagatcg ccatagcgat aatgagccgg gctgtcttcc ggtttccgct gaagtcgccg
     2821 gaacagagcc ttgatgcggg ccaccaaagt tttggggctg aacggtttgg tgac
//
LOCUS       DATIPF010000166         3392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_119230, whole genome shotgun sequence.
ACCESSION   DATIPF010000166 DATIPF010000000
VERSION     DATIPF010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119230"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VLL06_04315"
     CDS             <1..552
                     /locus_tag="VLL06_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HSS30222.1"
                     /translation="MAAVCEGNSVDTSMGFTPTAGLPMSTRSGDLDPGLLSYLSRTEG
                     MTVEQFHAMVNQQSGLLGLSETSSDLRDLLASERHDPRAAEAVALFCYQAKKWIGSFT
                     AALGGLDTLVFSGGIGENSPVVRARICEGLGCLGVVLDEAPNSMNAAIISKEGSGVTV
                     RVIPTNEESEIVSSVLQLLDQTR"
     gene            621..3011
                     /locus_tag="VLL06_04320"
     CDS             621..3011
                     /locus_tag="VLL06_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HSS30223.1"
                     /translation="MYQSKKKPLSADLLRNIHAYWRAANYLSVGQIYLFDNPLLKKPL
                     TLAHIKPRLLGHWGTTPGLNFIYVHLNRIIKKYDLNVMSITGPGHGGPGLVANAYLEG
                     TYSEVYPNISQDEEGLKRLFTQFSFPGGIPSHVSPETPGSIHEGGELGYSLSHAFGAA
                     FDNPDLLVACVVGDGEAETGPLATSWHSNKFLNPVRDGAVLPILHLNGYKIAGPTVLA
                     RIPHDELKSLLTGNGYKPYVVAGDNPNVMHQQMAATLDAVVADIKRIQRAARTKGFHT
                     RPRWPMIVLRSPKGWTGPKAVDGKQIEGTFRSHQVPMGDMSHPGHVELLEQWMKSYRP
                     EELFDRTGRFLPEIADLAPRGERRMSANPHANGGLLLQDLHMPDFRDYALNIQKPGVV
                     KGEATRVQGEFIRDVVSSNSNNFRIFSPDETNSNRWGAVFEVTNRCSTAEIIPGDDHV
                     APDGRVMEMLSEHQCEGWLEGYLLTGRHGMFSCYEAFIHIVDSMFNQHAKWLKVCGHI
                     PWRRPIASLNYLLSSHVWRQDHNGFSHQDPGFIDHVVNKKAEVIRVYLPPDANTLLSI
                     TDHCLRSRNYVNVIVAGKQLAPQWLNMDDAIAHCTAGLGIWPWASNDQGSEPDVVMAC
                     CGDVPTLETLAAVELLRIWLPRLKVRVINVVNLMKLQPRSEHPHGLTDNEFDRLFTTD
                     KPIIFAFHGYPWLIHRLTYRRTNHKNLHVRGYKEEGTTTTPFDMVVLNDLDRFHLVMD
                     VIDRVPMLGHLAADTRQAMQDKRVEHKAYIAKHGDDQPDIRDWQWTAARSGSQSHS"
     gene            3030..>3392
                     /locus_tag="VLL06_04325"
     CDS             3030..>3392
                     /locus_tag="VLL06_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSS30224.1"
                     /translation="MTVPRIVFLFDVDNTLLDNDRVTADLKYHLEQTVGRERQENYWA
                     IFERLRMDLGYADYLGALQRYRIEHPRDPQVLTVSHFLVNYPFANRMFPNALDAVEHV
                     KQWGKAVILSDGDVVFQPR"
BASE COUNT          745 a          906 c         1011 g          730 t
ORIGIN      
        1 atggccgcgg tgtgcgaggg caacagtgtg gataccagca tggggtttac gcctacggca
       61 gggctgccga tgagcactcg ttcaggcgat ctcgacccag gtctgctgtc ctatttgtcg
      121 agaacggaag gcatgaccgt agagcagttc catgcgatgg tcaatcaaca atcggggttg
      181 ctcgggctgt cggagaccag ttctgatctg cgcgatctct tggcaagcga acgtcacgat
      241 ccacgtgcgg cggaagccgt ggcgctcttc tgctaccagg ccaagaagtg gataggttca
      301 ttcactgctg cgttgggcgg attggacacg ctggtcttca gcggaggtat tggtgagaat
      361 tcgccggtgg tcagagcgcg catctgtgag gggctgggat gtcttggcgt cgtgttggat
      421 gaggctccca acagcatgaa cgccgcgatc atttccaagg aagggagcgg agtcaccgtt
      481 cgtgtgatcc cgacgaatga agagagcgag attgtgagtt ctgttcttca actgttggac
      541 cagacgcgat gatatagaga aactgctcac gtcggacacg tgaatcttct cggagctaga
      601 ccacttgcaa tgaggaatct atgtaccaga gcaagaagaa accactgagt gcagacctgc
      661 tcagaaacat acacgcctat tggcgggctg cgaattatct ctccgtcggc cagatctatc
      721 tcttcgacaa tccgttactg aagaagcccc ttacgctggc ccatatcaag ccgcgcctgc
      781 tcggtcattg gggcacgacg ccggggctga acttcatcta tgtccacttg aaccgaatca
      841 ttaagaaata cgatctgaac gtcatgtcta tcaccgggcc tggccatggt gggcccggtc
      901 ttgtggcgaa cgcgtatctg gaaggcacct acagcgaagt ctatccgaac atctcgcaag
      961 atgaagaggg gctgaagcgg ctgttcacgc aattttcctt tcctggcggc atcccgagtc
     1021 acgtctcacc ggagacgccg gggtctattc atgagggtgg ggagttggga tactcgctgt
     1081 cacatgcgtt cggcgcggcg ttcgataatc cggatctgct cgtcgcttgt gtcgtgggtg
     1141 atggtgaagc agaaacaggc ccgctggcga ccagttggca ttcgaacaag ttcctgaatc
     1201 ctgtgcgaga cggagccgtg ctgccgatct tgcatctgaa cgggtacaag atcgccggcc
     1261 ctacggtgct ggcgcgtatt ccgcacgatg aactgaaatc actgctcacc ggaaatgggt
     1321 acaagccgta tgtggtcgca ggtgacaatc ccaatgtcat gcatcaacag atggccgcca
     1381 cgctggatgc ggtcgtggct gacatcaaga ggatccagcg agccgcgcgg acgaagggat
     1441 ttcatacacg gcctcggtgg cccatgatcg tcctacgttc gccgaagggc tggactgggc
     1501 cgaaggccgt ggatgggaag cagattgagg gaaccttccg ttcgcaccag gtgccgatgg
     1561 gtgatatgag ccatccgggc catgtggagt tgttggaaca gtggatgaag agctatcggc
     1621 cagaggaatt gtttgaccgg acggggaggt tccttcctga gatcgccgat ctggctccga
     1681 gaggcgaacg ccgcatgagc gccaacccgc acgccaacgg cggacttcta ctgcaggacc
     1741 tgcacatgcc ggatttccgc gattatgcgc tgaacataca gaagcccggt gtcgtgaagg
     1801 gcgaagcgac acgtgtccag ggcgagttta tccgtgatgt ggtctcatct aactccaaca
     1861 acttccgcat attcagcccg gatgagacga actcgaaccg atggggcgcc gtctttgagg
     1921 tgacgaaccg atgctcaact gcggagatca ttccaggaga cgatcatgtg gcgccggatg
     1981 gccgcgtgat ggagatgttg agcgagcatc aatgcgaagg gtggctcgaa ggctatctct
     2041 tgacgggccg ccatggcatg ttctcctgtt atgaagcctt tatccatatc gtcgattcga
     2101 tgttcaatca acatgccaaa tggctgaagg tctgcggcca cattccctgg cggagaccga
     2161 tcgcgtcgct gaattatctc ttgtcctctc acgtctggcg gcaggatcac aacggtttca
     2221 gccaccagga tccggggttc atcgaccatg tagtcaataa aaaggctgag gttatccggg
     2281 tctatctccc acccgatgcg aataccctct tatccatcac cgatcattgt ctacggagcc
     2341 gcaactatgt gaatgtcatc gtggccggca agcagctcgc gccccaatgg ttgaacatgg
     2401 acgatgcgat cgcacattgc accgccggtc ttggcatctg gccgtgggcc agcaacgacc
     2461 agggtagcga gcctgacgtc gtgatggcct gctgcggcga cgtacccacg ttggaaacgc
     2521 tggctgccgt cgagttgctc aggatatggc tccctcgcct caaggtccga gtgattaacg
     2581 tggtgaactt gatgaagctt cagccgcgga gtgagcatcc tcacgggttg accgacaatg
     2641 agttcgaccg cctgttcacg accgacaagc ccatcatctt cgccttccat ggctatcctt
     2701 ggctgattca tcgcctgacc taccgccgga cgaatcacaa gaatttacat gtgcgcgggt
     2761 ataaggaaga agggacaacg accacaccgt ttgacatggt ggtgttgaac gatctcgatc
     2821 ggttccattt ggtgatggac gtgatcgatc gtgtcccgat gctggggcat cttgccgccg
     2881 ataccagaca ggcgatgcag gacaaacgag tggagcacaa ggcgtatatc gccaagcacg
     2941 gggatgatca gccggatatt cgagattggc aatggactgc agcacgatca ggttctcaga
     3001 gtcattcata gcccggcgcg gaggctccca tgacggtgcc acgtatagtg tttctcttcg
     3061 acgtcgataa cactctcctc gacaatgatc gggtgaccgc cgacctcaaa taccatcttg
     3121 aacagacggt gggacgtgaa cggcaggaaa actactgggc gatcttcgaa cggttacgaa
     3181 tggacctcgg ctacgccgac tacctggggg ccctccagcg ctatcggatc gaacatcccc
     3241 gcgatccaca agtattgacg gtctctcact ttctcgtgaa ctatccattt gcgaatcgaa
     3301 tgttcccaaa cgcgctcgat gcggtggagc acgtcaagca atgggggaag gcagtcatct
     3361 tgtcggatgg ggatgtagtg ttccagccga gg
//
LOCUS       DATIPF010000167         6786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1535657, whole genome shotgun sequence.
ACCESSION   DATIPF010000167 DATIPF010000000
VERSION     DATIPF010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6786
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535657"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /gene="erpA"
                     /locus_tag="VLL06_04330"
     CDS             <1..357
                     /gene="erpA"
                     /locus_tag="VLL06_04330"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HSS30225.1"
                     /translation="RKTAWSHEEEIRMVTITAIAEQKIKELMVEEKDIVGLRVYVKGG
                     GCHGYQYGMAFESKMADDDTVVEKGGVKVIMDSQSAPLLQGAEVDYVDSLQGSGFSIK
                     NPQAKTTCGCGSSFTA"
     gene            481..930
                     /locus_tag="VLL06_04335"
     CDS             481..930
                     /locus_tag="VLL06_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HSS30226.1"
                     /translation="MAQASVTRRYRFCAAHRLHTDHLSPEENLAVFGKCNNPNGHGHN
                     YVVLVTVRSPIPTATDKGLDLDQLDRIVRQQVITRFDHHDINRDPEFADRTTTGENLV
                     LLIWDLLVKCFPSGQLEKVGLIETRDNYFEYAGSGSQAQAVRGRQHG"
     gene            998..1570
                     /gene="folE"
                     /locus_tag="VLL06_04340"
     CDS             998..1570
                     /gene="folE"
                     /locus_tag="VLL06_04340"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HSS30227.1"
                     /translation="MDVLQSLVTEMLLALGEKPGRNGLLKTPERVAKALGFMTQGYHR
                     DIDQLLNGALFPIEYDEMVIVKDIDFFSMCEHHLLPFYGRVHVGYLPNKKVVGLSKIP
                     RIVDVFARRLQVQERLTVQIAETLKSKLNAHGVGVVVEARHLCMMMRGVEKQNTIAVS
                     SSMLGAFRSQPQTRLEFLKLIHQGSVGDSD"
     gene            complement(1831..2625)
                     /locus_tag="VLL06_04345"
     CDS             complement(1831..2625)
                     /locus_tag="VLL06_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSS30228.1"
                     /translation="MKTHVNGITIASSDQGTGLPIVFLHAFPLNRTMWAAQENALSSQ
                     FRIITIDLRGHGESDAPLWRYTLEQSAGDVSALLGHLEIRRALFVGLSMGGYILFAFY
                     RRYAARVKGLILADTKAQADTEEGKNGRFQLAQIAYKKGPSAIADVMIPKLLSPATIQ
                     TNPELVQRVRAMIEGNQTSGIAWDLMAMAERPDSVPLLSRITCPTQIIVGELDQATPP
                     SDSKLMAEHIPHARLAIIPHAAHLSNLEQPEAFNQIVSAFALNLEK"
     gene            2746..4038
                     /locus_tag="VLL06_04350"
     CDS             2746..4038
                     /locus_tag="VLL06_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HSS30229.1"
                     /translation="MTTSHDQAPDLFGTPEGGKSVEAPLAERLRPREFGDFVGQEDIT
                     APDRPLRRAIEADQLSSVIFWGPPGSGKTTLAHLIARYTKAQFVSFSAVTGGVPELRT
                     IIKAAEQRQAINGQRTILFVDEIHRFNKAQQDAFLPHVERGTLILVGATTENPSFEVI
                     SPLLSRSLLVVLKPLSDEALGLILGRALTDPAIGLGKWNARLSPDARQRLIAYGNGDA
                     RALLTALDFVVTQQPVGTDGSRLIDGAALEAALMRKSIRYDKSGEEHYNVISAYIKSL
                     RDSDPDGALYWLARMLEGGEDPKFIARRLVIFASEDVGNADPMGLVVATAVAQAVQFV
                     GLPEAQINLAQGTTYLATRQKDNASYIGLLEALEDTKAHGNLGVPLHLRNAVTSMMKG
                     LGYGKGYRYVHDDPQAKGQQEHLPESLKGRRYYRPKEP"
     gene            4094..4483
                     /locus_tag="VLL06_04355"
     CDS             4094..4483
                     /locus_tag="VLL06_04355"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS30230.1"
                     /translation="MKRQNVKSTTSIAATNERRRFVRATLVGSALISPKSGAKAITAV
                     LDNVNRVGAGLHTKEKLAMAQPVTVSLAFLDSDRAEQMEKLDGSVAWVKSWEKGFLIG
                     VVWDELVTKEKNRWLYYYLEETVKSSA"
     gene            complement(4677..6521)
                     /locus_tag="VLL06_04360"
     CDS             complement(4677..6521)
                     /locus_tag="VLL06_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HSS30231.1"
                     /translation="MLQIESVYKQYSTRILLEGATAHLRPGSRVGLVGPNGAGKTTLF
                     RMILEEESPDKGKIRKRPRLRIGYLPQELETITGKTVLDATHRDLYPEHEAERILMGL
                     GFSEIDFSREVEKLSGGYRMRVALAHLLLTNPDVLMLDEPTNHLDKPTQRWFEQFLLN
                     SEMTLLIISHDTAFLDRVVTHIWDLRHHKMEEYRGNYTSFKKTRAERDAQREAAAGRQ
                     AKEVARVQTFVDRFRYQANKASQVQSRIKQLEKVKMIEIKRDPKRVKFKFPLPAASGR
                     QVLDLQGAAKRYGEKVVYERVDCSIERGQRVALVGENGAGKSTLLKMLAGVLVLDKGT
                     RTVGHGVTLHYFAQHQAETLNPEHTILDSLSEVSSQAETNFLRGIAGAFLFTGDDQKK
                     PIKALSGGERNRVALARMLVEPANTLLLDEPTNHLDPASVDMLTDALSDFPGTIVFIS
                     HDPTFLTRVCTRVIEIEEGKARSFSGDYEYYLWKKAQEIESIKETSDDLRGTDRGKTD
                     APTRAMASQAPAKSSAGDRRDLNKTHARLEKQVTRAETEIAEYETKIKARDLELADPA
                     LYKDESTKWSALHLEHEGWKTELVRLTARWEMLSAELEDVKQKLTAFA"
     gene            complement(6625..6700)
                     /locus_tag="VLL06_04365"
     tRNA            complement(6625..6700)
                     /locus_tag="VLL06_04365"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6665..6667),aa:Ala,seq:ggc)
BASE COUNT         1438 a         1743 c         2023 g         1582 t
ORIGIN      
        1 cgaaagaccg cctggtcaca cgaggaggag attagaatgg ttacgattac ggcgattgca
       61 gagcagaaga tcaaggaatt gatggtggaa gagaaggata tcgtcggctt gcgggtctat
      121 gtgaagggtg gtggatgtca cggctatcaa tacggaatgg cctttgagtc gaagatggcc
      181 gacgacgaca ccgtggttga aaagggtggc gtcaaggtca tcatggactc gcagagcgct
      241 ccgctccttc agggagccga agttgactat gtggacagtc tccaggggtc cgggttctcg
      301 atcaagaatc cacaagccaa gaccacctgt ggctgtggca gctccttcac tgcctaacga
      361 atttgtgctg gagcggtgcg agccggtcag tgaactatat aaagaatgaa gccagggctg
      421 tccgaatact gcggccggtc ctggcttctc attttgcgat ccatgtgagt tgaatctaca
      481 atggcacagg cgagcgtcac aaggcgatat cgattctgcg cggcgcaccg gctacacacg
      541 gatcatctgt ctccagaaga aaacctggca gtattcggga agtgcaataa tcccaacggg
      601 catgggcata actatgtcgt gttggttaca gtgaggagtc cgattccgac ggcgaccgat
      661 aaaggcctgg atctcgatca gctcgatcgg attgtgagac aacaagtcat cacgcgattc
      721 gaccatcacg atatcaatcg cgatcctgag tttgccgatc gcacgacgac gggtgaaaat
      781 ctggtgttgc tgatttggga cctgttagtg aaatgtttcc ccagcgggca attggaaaag
      841 gttgggctga ttgaaacgcg cgacaactac ttcgagtatg cggggtcggg gagtcaggct
      901 caggcggtta gagggaggca gcatggctaa gcagggaatc acgcggcgac gtcagcggca
      961 ggtcgaagac gtcaaagtga atcgcggacc ggcagatatg gatgtgttgc aatccctggt
     1021 cacggaaatg ttgctcgccc ttggggaaaa accaggacgg aatgggctcc tcaagactcc
     1081 cgagcgagtg gccaaggccc tcggcttcat gacccagggc tatcaccgcg acatcgacca
     1141 gctcttgaac ggtgcgctgt tcccgatcga gtacgacgag atggtcatcg tgaaggacat
     1201 cgatttcttc agcatgtgcg agcatcacct gctgccgttc tacggcagag ttcatgtggg
     1261 ctatttgccc aataagaagg tggtggggct cagcaagatt cctcgcatcg tggacgtctt
     1321 tgcgagacgg ctgcaagtcc aagaacgact gaccgttcag atcgccgaaa ccctgaagtc
     1381 gaagctcaat gcgcatggag tgggcgtggt cgtcgaagcg cgccatcttt gcatgatgat
     1441 gcggggtgtc gaaaaacaaa acacgatcgc ggtcagtagc tccatgttag gcgcgttccg
     1501 cagtcagccg cagaccaggt tggagttttt gaagttgatt caccaaggca gcgtcggcga
     1561 ctccgattaa gcaggacgtt gaaaaagtcc cccagctgcg ttctcgccta gaaagcatcc
     1621 tcaacgtagc caagaggcta cgcctccggt gcttccattg gctgcggcct tgctggatga
     1681 actttttgaa cgtcctgcat ggtctctccc atttcatccc atccattccc gtcttcagtt
     1741 tcccaaaaaa cttgccccag actctcgcta gctatggtct tgttggatga gcggcttccc
     1801 tattctcgcc acaggcagtt ggtctcttac ttacttttcg agattcagag caaaggccga
     1861 gacgatctgg ttgaaggctt ctggttgttc tagattcgac aggtgtgcgg catgtgggat
     1921 gatggccagg cgggcgtggg ggatgtgttc ggccatgagc ttcgaatcag aaggtggagt
     1981 ggcttggtcc agttcgccga cgatgatctg tgtgggacag gtgatccggc ttaagagcgg
     2041 aactgaatct ggccgttcgg ccattgccat gaggtcccac gcaattccgc tggtctgatt
     2101 tccctcaatc atggctctca ctcgctggac gagctcgggg ttcgtctgga tggtcgcggg
     2161 gctgaggagt tttgggatca tgacgtctgc gatggcagac ggtcctttct tataggcgat
     2221 ctgcgcgagt tggaatcgcc cgtttttccc ttcttctgtg tctgcctggg ccttggtatc
     2281 tgccaggatc agtcccttca cgcgagcggc gtacctccga tagaacgcaa acagaatata
     2341 gccgcccatc gaaagcccca cgaacagggc ccgccggatt tcgagatggc ccagcaatgc
     2401 gctcacatcg ccggcggatt gttccaacgt atagcgccag agcggcgcgt cagattcgcc
     2461 atgccctcga aggtcaatcg tgatgatccg gaactgggac gagagcgcgt tctcttgcgc
     2521 tgcccacatg gttcgattca gcggaaaagc atgcagaaag acgatgggga gaccagtccc
     2581 ttggtcgctg gaggcgatgg tgatgccgtt cacgtgggtc ttcatcgttt ctccactgtg
     2641 cgcgcccttc gtagcattgg tcgtcggcag cgtcaagagc gggcggtgct tgtttgctct
     2701 ggtgggagga ggcgtataga attcccgcaa ttgctgagga cctgcatgac cacatcacac
     2761 gatcaggctc ccgatttgtt cggtactcca gagggaggga agtccgttga agctccgttg
     2821 gccgagcgac tgcggccgcg agaatttgga gacttcgtcg ggcaagagga tattactgcg
     2881 ccagatcggc cgttgagacg ggcgatcgaa gcagatcaac tttcctccgt catcttttgg
     2941 gggccgccgg gctcggggaa aacgacattg gcgcatctta tcgcgcgcta cacaaaggcc
     3001 cagttcgtgt ccttctcggc ggtcacaggg ggtgttcccg agctgcggac catcatcaag
     3061 gctgcagagc aacgtcaggc aatcaatggt caacggacca tcctctttgt cgacgagatc
     3121 catcggttca acaaggcaca gcaagatgcg tttctgcctc atgtcgagcg ggggacgctc
     3181 attctggtcg gagcgaccac ggagaatccc tcatttgaag tgatctcacc gctgctgtct
     3241 cgttccttgc ttgttgtgct taagcccctt tcggatgaag ctctggggct gattctgggc
     3301 cgggcactca cggatcctgc gatcgggttg ggaaagtgga atgcccgcct gtcacccgat
     3361 gccaggcaac gactgattgc ttatggcaat ggcgatgcga gggcactcct gacggcgttg
     3421 gattttgtgg ttacacagca accggttggg actgacggga gtcgactgat cgatggtgcg
     3481 gcgttggaag ccgcgctgat gaggaagtcg atccggtacg acaaatccgg cgaagaacat
     3541 tacaacgtca tctcggcata cattaagagt ctgcgcgatt ctgatccgga tggtgccctc
     3601 tattggctcg cccgtatgtt ggaagggggg gaggatccga aatttatcgc ccggcgactg
     3661 gttatttttg cttccgagga tgtggggaat gccgatccga tggggctggt ggtggcgact
     3721 gcggtcgctc aggccgtaca gtttgtgggg ctcccagagg cccaaatcaa tttggcccag
     3781 gggacgacgt atctcgcgac caggcaaaaa gataatgctt cctatattgg actgcttgag
     3841 gccctggagg atacaaaagc ccatggaaat cttggagttc cgctccattt acggaatgcg
     3901 gtgacctcca tgatgaaggg gctcgggtac ggaaaagggt atcgctatgt ccatgacgat
     3961 ccccaggcca agggacagca agaacatctt ccagaatcgc tcaaggggcg tcggtattat
     4021 aggcctaaag agccctaaat aggggcatca caacgtggtg gacaaagatt tctaatcggt
     4081 tatacttagt cttgtgaaac ggcagaatgt caaatccacg acatcaatag ctgccacgaa
     4141 cgaacggcgc aggtttgttc gagcaacgtt ggtcgggtct gcgctgattt caccgaagag
     4201 cggagccaaa gccattacgg ccgtgttgga taacgtcaac agagtcggcg cagggctgca
     4261 cacgaaagag aagttggcga tggctcagcc ggtgaccgta tcgttggcgt ttctcgattc
     4321 ggatcgtgcg gaacagatgg agaagctcga cggaagtgtc gcctgggtga agtcatggga
     4381 gaaggggttt ctgattggtg tggtatggga cgagttggtg acgaaagaga agaaccgctg
     4441 gctgtattac tatctcgaag aaacggtcaa gtcctccgcg tagcaggatg ctgaaaaagc
     4501 ccgccagcat cgttctcgca tcgttcagat cctcaacgta ccccagaggg tacgcctgcg
     4561 gtcttcactc gctgcggcct tgctgacagt ctttttgagc atcctgctgc agtgttcgca
     4621 ctcagcgtgt gggtcattca agttcttacc tgccgcaata cttttcctac acaagactat
     4681 gcgaaggccg tcagcttttg tttgacatct tccagttcag ccgagagcat ttcccaccgg
     4741 gcagtgagcc gcactagttc cgtcttccac ccttcatgtt ccaggtgtaa agcgctccac
     4801 ttggtagatt catccttgta gagtgcggga tcagccagtt cgagatcccg cgctttgatt
     4861 ttagtttcgt attcggcgat ctccgtttcg gcccgcgtga cctgcttttc cagtcgagcg
     4921 tgggtcttgt tcaaatcgcg gcgatctcct gctgaggact tggccggagc ctgcgaagcc
     4981 attgccctag ttggcgcatc agttttccca cggtccgttc ctctcaggtc gtcactcgtt
     5041 tccttaatgg actcgatttc ctgcgctttc ttccataagt aatactcgta gtcgccggaa
     5101 aagctgcggg ctttgccctc ttcgatttca atgacgcgcg tgcagactct ggtcaagaag
     5161 gtgggatcgt gagaaatgaa gacgatcgta ccagggaagt ctgatagcgc atcggtgagc
     5221 atatcgactg aagccgggtc gagatggttc gtgggctcgt cgagcaacag ggtattggcc
     5281 ggctcgacca acatcctcgc gagtgcgacg cgattgcgtt cccctccgct cagcgccttg
     5341 attggtttct tttgatcgtc cccggtgaac aggaaggcgc cggcaatccc gcggaggaag
     5401 tttgtctccg cttggctgga cacctcggaa agggagtcga ggatggtgtg ttccgggttc
     5461 agtgtttcgg cctggtgttg ggcgaagtag tgcagggtga caccatgacc gaccgttctg
     5521 gtgcctttgt cgaggaccaa cacgccggcc agcatcttga gcagcgtgct tttcccggca
     5581 ccgttttccc ctaccagcgc cacgcgctga ccacgttcga tcgaacaatc aacgcgttca
     5641 tacaccactt tttctccata gcgttttgct gcgccttgga gatccaatac ctggcggcca
     5701 ctggctgcgg gaagcggaaa tttgaacttc acccgcttgg gatcgcgctt gatctcaatc
     5761 atcttgacct tttcaagctg tttgatgcgc gattggacct ggctggcttt gttcgcctga
     5821 tagcggaaac gatccacgaa ggtttgaacc cgagcgacct ctttcgcctg gcgcccggcg
     5881 gctgcttcac gctgagcatc ccgctctgcg cgtgtctttt tgaacgaggt atagttgccg
     5941 cggtactctt ccatcttatg gtgtcgaaga tcccagatgt gggtgacgac acgatcgaga
     6001 aacgcggtgt cgtggctgat gatcaacaat gtcatctccg aattcaacag gaattgctcg
     6061 aaccagcgct gcgtcggttt gtccaggtgg ttggttggct catcgagcat gaggacgtcg
     6121 gggttggtca ataggaggtg cgcgagggcg acacgcatcc gatagccgcc ggacagtttt
     6181 tcaacctcac ggctaaagtc gatttctgaa aatcccaacc ccataagaat gcgttcggct
     6241 tcgtgttcgg gatagagatc tcgatgggtg gcgtccagca cggttttgcc cgtgatggtt
     6301 tcgagttctt gagggagata gccgatgcgg agacgaggac gcttccggat ctttcccttg
     6361 tccggcgact cttcctcaag gatcattcgg aagagggtgg tcttgccggc gccattgggg
     6421 ccgaccaacc caacccgcga accagggcgg agatgtgccg tcgcaccctc aagcagaatt
     6481 ctcgtggaat attgtttata gaccgactcg atttggagca tgagatgttt tgtgaaccag
     6541 gcggattgaa cttaccagga ggtgccgatg gtcattcggt gattaggtgt gatgtcgagg
     6601 ttcgtagaac ctacgtcgtt aatttggtgg agctggccgg gattgaaccg gcgacctcgt
     6661 gaatgccatt cacgcgcgct cccaactgcg ctacagcccc acaacagttg atttggaaat
     6721 aggcgaaaat ccttgtgaaa caacgatgat ggtaacatgg ccctccttcg acagtcaaga
     6781 aaacag
//
LOCUS       DATIPF010000168         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1446576, whole genome shotgun sequence.
ACCESSION   DATIPF010000168 DATIPF010000000
VERSION     DATIPF010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446576"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..402)
                     /locus_tag="VLL06_04370"
     CDS             complement(82..402)
                     /locus_tag="VLL06_04370"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HSS30232.1"
                     /translation="MRERCCSAWSRWLFVLVLVIPVCGVLPTFAVAVEQTGVKVTAIE
                     VRGNKRIEMPAIVGRLTLKPGDPYTPENVRGQIKILYETGFFEDVQVETESGAQLRSH
                     TPPS"
     gene            933..1844
                     /gene="galU"
                     /locus_tag="VLL06_04375"
     CDS             933..1844
                     /gene="galU"
                     /locus_tag="VLL06_04375"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HSS30233.1"
                     /translation="MSRFEVRKAVIPAAGMGTRFLPATKASPKEMLPLVDKPLIQYAV
                     EEAVASGIEDIIIITGRGKRAIEDHFDRSVELEEILKGSGKAQLLSQMRHISSLANFC
                     YVRQTEALGLGHAVLCAQRLIGDEPFAVMLGDEIIDAPVPGLAQLIHAYKKRHGAILG
                     VQEVPHQDVNRYGIVSSRKIAAGLHRVEDLVEKPVPEEAPSNLAVIGRYVLPPEIFSI
                     LRKTRPGKNGEIQLTDALRELAKKSPMHALEVQGQRYDAGDKLGFLIATVEFALKNPS
                     LGPDFGDYLQNLIRPSEGRHTTRSRKS"
BASE COUNT          494 a          595 c          518 g          450 t
ORIGIN      
        1 ggggggactc acctcggcca gccgtgaagt gatccgcccg gtcctgcgtc gattcaacac
       61 gctctatttc tacaatacgc tttatgaagg cggcgtgtgc gaccgcaact gcgctcccga
      121 ttccgtctcc acctgcacgt cttcaaagaa cccggtttcg tagagaattt taatttgtcc
      181 acggacattt tcaggggtgt acggatcgcc aggtttcaat gtcaatcgcc cgacaattgc
      241 cggcatctcg atccgcttgt ttccgcgaac ctcaatcgcc gtcactttga ctccggtctg
      301 ctccaccgca acggcaaacg tcggcagcac gccgcagact gggatgacga gcaccaagac
      361 gaagagccac cgagaccagg ctgaacaaca tctttccctc actggatctt cgttccacag
      421 aaaaagaaga aaaataatac cggcattatg ggtaacccta gggtggagct gtaaacgtac
      481 gcgctgcccc actagcctaa tcacacacgt cgcagccggg gagcgcgtga ggatgtcggt
      541 acagaaggct actgacgtgg gaggttctgg cggttaacgc acacgaaccc ctcacggcaa
      601 catgagcgca aaacctctgg attatattgg gcaagtattt gaatgtaaag ctgagagcct
      661 acattattat tcgcgcaaga tccttgcgtg acccatcgta tttctcttct tgatgtggtc
      721 cgtttggttt tttggttgaa taagatgagg cagatacaca ccacatactg gatacatcag
      781 atcaccccgc ctcatgtgag tactgtatct gcacgtgtga cagataggcc atgcatcatg
      841 tggacaactc cttggttgcg caactgtgct cgtgaaactt gtaagctcct ggattccttg
      901 acatcgccta ccccatcaca tagtatcgaa tcatgtcacg tttcgaagtg cgcaaagctg
      961 tgattcccgc tgcaggaatg ggcactcgct tccttcctgc gacgaaagcc tctcccaaag
     1021 aaatgttgcc gcttgtcgac aagccattga ttcaatatgc cgtggaggaa gcggtcgcct
     1081 ctggaatcga agacattatc attatcaccg gccggggcaa acgcgcgatc gaggaccatt
     1141 ttgaccggtc cgtcgagttg gaagaaatcc tcaagggtag cggcaaggcg caactcctga
     1201 gccaaatgcg tcacatctcc tcactcgcaa acttctgtta cgtccggcag accgaagcgc
     1261 tcggactcgg gcatgcggtg ctctgtgccc aacggttgat cggtgacgag ccctttgcgg
     1321 tcatgctcgg cgacgagatc atcgatgccc cggtccctgg cctcgcacaa ctcattcatg
     1381 cctacaagaa acggcatggg gcgatcctcg gcgtgcaaga agtcccccac caagacgtca
     1441 accgctatgg gattgtgtcg tctcgcaaga ttgccgcggg attgcatcga gtcgaagatc
     1501 ttgtcgagaa accggtgccg gaggaagctc cgtccaattt ggcggtcatc ggtcgctatg
     1561 tcctaccacc ggagattttc tccatcctga gaaaaacccg tcccggcaaa aatggcgaga
     1621 tccaattgac cgacgcactt cgggaattag ccaagaaatc cccgatgcat gcgctggaag
     1681 tacagggaca gcgctatgat gccggagaca agttaggatt tctcatcgcg accgtcgaat
     1741 tcgcactcaa gaacccctcg ttagggccgg acttcggcga ctatctgcag aatctgatac
     1801 ggccttccga aggacgccat accacacgat cacgaaaaag ctagtcacgg cattcaacga
     1861 agaaggcacg acctcataca tctcattcag cactgcgcac cacactgaga ccctatcgat
     1921 gaacgattcg actgaacaag acctgcagcc gccacagaat atcgaaatcc ccgaccagct
     1981 tccgctgttg ccggtccgag acatcgtcgt cttcccttac atggttctcc cgctgtttgt
     2041 cgggcgcgaa atgtcga
//
LOCUS       DATIPF010000169        12307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_298087, whole genome shotgun sequence.
ACCESSION   DATIPF010000169 DATIPF010000000
VERSION     DATIPF010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 12307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12307
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298087"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VLL06_04380"
     CDS             <1..298
                     /locus_tag="VLL06_04380"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSS30234.1"
                     /translation="HTDVAVTAWDGDLLVGFGRVLTDYVYRATIWDVIVDKACQDQGV
                     GTEIVHRILTHPRLKKVELFWLCTRIPDFYEKLGFSSKEQTGMVWSRSKQGRLE"
     gene            complement(422..1024)
                     /locus_tag="VLL06_04385"
     CDS             complement(422..1024)
                     /locus_tag="VLL06_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSS30235.1"
                     /translation="MVLSGLVLSGQAKAEEWKMEQGGIEPGKVMLGMRAGFAPLTQTV
                     SQGTSTDVGSLVNFQGMYSLNNWLLAGMMVEWERHGMSNERPDIDLGHQDTVSVLPTV
                     EVRPVKFGPLVPYANMSVGINVNSFGESSAIRGATISPSNNFAWRLGWGADYMLTKQF
                     AVNTEMAYKRNDGHATIDGVRINDWNASSFGFLVGVKLFF"
     gene            complement(1335..2159)
                     /locus_tag="VLL06_04390"
     CDS             complement(1335..2159)
                     /locus_tag="VLL06_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HSS30236.1"
                     /translation="MLELFAYDFMQRSLLAAAMVGGLCSVIGVFVVLRGLAFMGAGTA
                     HAAFAGVALGFLMGWPPLLMALLFGLATVWTTGWMEEKGRMKLDVSIGILYTATMALA
                     ILFIGLMKTYNAEVYGYLFGNVLAVTSEELLTIAALSFVVIGAIVLFSKELYFIAFDQ
                     EMAEASGVPARRIFFLLLTLVALTVVISLKTVGAILVFAMILIPASTAYQLTHSLSTL
                     TWYSAIIGVSCSVGGVLISSYWDIPSGPAIVLLATLMFFVAVLLSPKRQRRSLAIN"
     gene            complement(2172..2963)
                     /locus_tag="VLL06_04395"
     CDS             complement(2172..2963)
                     /locus_tag="VLL06_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HSS30237.1"
                     /translation="MTSQSLPIIHFDHATFGFPGVVALEDISLDIQSGEFVGVIGPNG
                     SGKTTLCRAVLGLMAPLKGHLRIFDCACDELRCHHRARIGYLPQKGVIDRNFPVTVLE
                     TVMMGRYGALGVFSRPSGKDRKIAIDALAHVGMEGHRDSALGHLSGGQQQRVFIARAL
                     AQQPDVLLLDEPTTGLDITTQHNVVELVQHLHEELGLTVLLITHDINMIRSRVNRLVL
                     LKTRLYAAGPPAEVLKADILSQVYGKELVITEKDLVIVEDYHHHH"
     gene            complement(3017..3964)
                     /locus_tag="VLL06_04400"
     CDS             complement(3017..3964)
                     /locus_tag="VLL06_04400"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HSS30238.1"
                     /translation="MLASSPMLNVQLVRLLWVILVSHIVFAPPLVLAASSAEPLNIVV
                     TIPVLKDLTEQVGGSHVRVTSLLSGYENEHTYSPKPSDLVAVRKAKLLFEVGIGLEIW
                     VSSLVKNAGSASLRVVTTSKGIALLRDRPEHDGAPHAAEENERGNPHVWLDPENAMTM
                     MRHITEALIQVDPAHATEYRTNQASYLRKLDQLRGELNERITRVSDRRFIAHHPAWPY
                     FARRFGFQIVGTIQPQSGSEPSALHLHELIAKIKKDQIKVVVSEIQLSQKTPELLAKE
                     TSARVVVLTTLPGGLPHTDSYLDMLRYNVLQLVNALDAA"
     gene            4249..5427
                     /locus_tag="VLL06_04405"
     CDS             4249..5427
                     /locus_tag="VLL06_04405"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HSS30239.1"
                     /translation="MHVVGLMSGTSGDGVDAALVDITGRGRALKARTLAAHTLAYPRS
                     LQQRILSASVSGTVAEVCHLNALLGEWFANAALLVIRQANLQPSDVALIGSHGQTVHH
                     LPHGIHAPGVGAIRSTLQIAEPAVIAERTGITTVANFRSRDVAAGGQGAPLAPVAHAL
                     LLKHARRARLVVNLGGISNVTYVPKGGHLIGMQAFDTGPANMVLDSLMARVTNGRLLM
                     DRDGKLAMKGQVDSRLLGKLLAHPFLSKRPPKSTGREAFGPDVIEEVIAIQQQRRLSI
                     EDLLATCSMWTAKAVGTSRRWIEGEIDEVVVGGGGVRNRAIMSHLATVFAPIPVTTFE
                     ALGWDSKVFEAVAFALLAYQTVTGQWGNIPSVTGASHPVLLGTIVPNGPRWRESLPGR
                     "
     gene            5435..5947
                     /locus_tag="VLL06_04410"
     CDS             5435..5947
                     /locus_tag="VLL06_04410"
                     /inference="COORDINATES: protein motif:HMM:NF022337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HSS30240.1"
                     /translation="MEIVFIGLAITCLVFLVWRLRVSSNTGLARTDAFVLPTGVTLRP
                     QPLLTDADLLLYNLIRLAVEDHYLVFPRVPLWSVVSVEAEGTVRSQVLRQIALKQLDF
                     VLVHPGTKAAEQVVLLEDSLPSQPHEVSRRRDIESVLQAAGIALTTLKPHTSYTVPQL
                     AQLLGVSEDE"
     gene            complement(5997..6509)
                     /gene="ybgF"
                     /locus_tag="VLL06_04415"
     CDS             complement(5997..6509)
                     /gene="ybgF"
                     /locus_tag="VLL06_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HSS30241.1"
                     /translation="MKALMIVIPGMIAVLFSVNLAVAAPSNMQDSSRHLYDRVMQEFK
                     HRDYEAALAGFRFFLELHGQSSLAANAQYWVGECQYRLGRYKEALKSFHHVVSYYPLS
                     PKLVASTLKIGQTYTRLKDHEKARMMYERVVDQYPESAEAELARKALDAAPMRDEELT
                     PQLTGNSSSD"
     gene            6880..7668
                     /locus_tag="VLL06_04420"
     CDS             6880..7668
                     /locus_tag="VLL06_04420"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSS30242.1"
                     /translation="MRIEPPPNPLGGKTLIVDANDGDSYVRPSAALLDAGEHDQVFVR
                     PGIYEDKVFITGRPIHLVGAGRDEVQIFSRRGGPLYLQQVPSGRISGITFRYVGSDQN
                     SAMNLLDSSCTVTQCRAMEGILSGVVIYGPQSRLTFVENEVCGNRESGIFVFAGAQPR
                     IADNLCRSNHHFGIAVRDPGSHPELVRNRCRENMLSGILLFHHAEALLVDNTCSENQH
                     WGMVMTPDCHPTPNREALDTSNILAPNPRGTLIVTDQPLNDIGR"
     gene            complement(7771..8694)
                     /locus_tag="VLL06_04425"
     CDS             complement(7771..8694)
                     /locus_tag="VLL06_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HSS30243.1"
                     /translation="MIKVLLIEDNDVDAQLTQDLLSEWSSEQFQVSRVKTLGEGLTYL
                     SRERFDAVLLDLSLPDAFGLPTVRQIHDTSPTIPVVVLSGVSDQSLALQAVQQGAQDY
                     LVKGQGHPELLARAVRYAIERKRTEERLTYLAQYDHLTGLVNRTLFRDRLVQAMARSK
                     RMHQPIGLMLLDLDRFKAVNDTYGHDIGDELLKAVSERLKTCVREVDTVARMGGDEFT
                     IILEGVSSDENILVVAKRITESIATIFELQGHHISVGVSIGITIYPHDDHPVDELLKH
                     ADTAMYRAKKQGGSAFHLHEASGTHSANLLP"
     gene            complement(9014..9397)
                     /locus_tag="VLL06_04430"
     CDS             complement(9014..9397)
                     /locus_tag="VLL06_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30244.1"
                     /translation="MEDLVIEEEGASSALKITLGDFLVFINWMNQTEKSLDRAEALPT
                     LPDVDSGKVKGWIQLVQRLEERFRRSEEKKLALEANVIAQEAQLGQLLSHLHANIASL
                     YDNLGQSQKAEDVRRRAEALHVGSY"
     gene            complement(9741..10061)
                     /locus_tag="VLL06_04435"
     CDS             complement(9741..10061)
                     /locus_tag="VLL06_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30245.1"
                     /translation="MTRGLLLLMVLLLAGCAESYTVFHSQTGEPILLGRRAYTYQGCL
                     STMRDEAARLGISLAMVQVRGSWQGRSLLWPFEPGYACDGTIGSERLPRGIYPGVPPL
                     SPQG"
     gene            complement(10096..10452)
                     /locus_tag="VLL06_04440"
     CDS             complement(10096..10452)
                     /locus_tag="VLL06_04440"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS30246.1"
                     /translation="MTKLRYCERIHAQFPVVFAGEAYVGEGTVLNVSVPGCAVHSRHR
                     VQPGSYLEMRMLVPDTMSPLRVGLAKVRWCEGRRFGVEFIQMPGEDQVRLGRLVKHEV
                     ALALPSRSSAWKGTQS"
     gene            11064..11522
                     /locus_tag="VLL06_04445"
     CDS             11064..11522
                     /locus_tag="VLL06_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HSS30247.1"
                     /translation="MGVGDLELEQAVRRAAQQHRVQPALLLAVMRAESSFNPTAISKA
                     GAVGLMQLIPETAIRHGVRNLYDANENIAGGAKHLRYLLDRFHGNVRLAVAAYNAGER
                     KVDRYGQIPPYKETQDYVKKVLVYYRSYKKDGWIMPVVTATPLTHVGRPY"
     gene            complement(11600..11797)
                     /locus_tag="VLL06_04450"
     CDS             complement(11600..11797)
                     /locus_tag="VLL06_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30248.1"
                     /translation="MHFVRIGKKALNLDSVSYCEAQIWQDEMSLKVHFVGSANNTPLV
                     LAEAEAKELWKYLEYAAEKPV"
     gene            complement(12038..>12307)
                     /locus_tag="VLL06_04455"
     CDS             complement(12038..>12307)
                     /locus_tag="VLL06_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSS30249.1"
                     /translation="PEYAPVTYYEILTLDEVLDPQTILAYEMNWRPLPIPHGAPCRLR
                     IETKTGYKMVKYLRTIEVVDTYATIGEGHGGYREDHQYYDKVAAI"
BASE COUNT         2927 a         3278 c         3329 g         2773 t
ORIGIN      
        1 ccacaccgat gtggccgtca cggcctggga cggtgacctg ctggtcggat ttggacgggt
       61 cttaacggat tatgtctacc gagcaaccat ctgggacgtg atcgtcgata aggcttgcca
      121 ggatcagggc gtcggcaccg agatcgtcca tcggattttg actcaccccc gcctcaagaa
      181 agtcgaactg ttctggctct gcacccgcat tcctgatttt tacgaaaagc ttggttttag
      241 ctcaaaggaa cagaccggaa tggtgtggag ccgatcgaaa caaggccgac tggaatagcg
      301 tattgtctcc gggtcgtagt gatcttccct ctgcatattc tgccaggact cgcatatccg
      361 gcgtggaacc cctgttctcc gggggttcca cgctatgaca tctgcttcgg atattcagaa
      421 actaaaagaa gagtttgacg ccaacgagga aaccaaaaga cgaggcgttc caatcgttta
      481 tgcgaacccc gtcgatcgtg gcgtgaccat cgttacgctt gtaggccatt tctgtgttca
      541 ccgcgaactg tttggtgagc atgtagtcgg ccccccagcc gagtcgccag gcgaagttgt
      601 tgctggggga aattgtggcg cccctaatcg cactgctttc cccaaagctg ttcacattga
      661 ttccaacgct catattcgca tagggaacga gcggtccgaa cttgaccgga cggacttcta
      721 ctgtcggcaa cacagacacg gtatcctggt gaccgagatc aatatctggt ctttcgttac
      781 tcatgccatg ccgttcccac tccaccatca tgccggctaa aagccaatta ttaaggctgt
      841 acatgccctg gaagttgacc agcgagccga catcagtcga agtaccttgc gaaacggttt
      901 gggtcaaggg ggcgaatcct gcacgcatac cgagcatgac tttgcccggc tctattccac
      961 cctgttccat cttccattct tccgccttgg cttgaccgct taagacaagc ccgcttagca
     1021 ccatgcaacc caccaaactc caacgagata ttacgagacc cattataata gccctccgtg
     1081 ctgtaatgat gtaaatcggc ctcagacatg cgttctctcc gcattagcaa cgcaagctta
     1141 gtgcccgatt tccccttggc taaattgtag tatgggaaac ggggttactc aatcaaaata
     1201 aaaaaggtct agacctatct acctcaggaa tcatgactcg cttccgacac actgttgtat
     1261 ctgccaccct acaggaggaa cgccagcatg aacgtacatg agaatgagat ggcgaacatg
     1321 acatggaatc gagattagtt gatggcgaga gaccgccgct gtctcttagg ggaaaggagg
     1381 accgcaacaa agaacatcag tgtggcgagc aacacaatgg caggaccgga gggaatatcc
     1441 cagtacgagg agatgagtac tccaccaacc gagcaggaga ccccaataat ggccgaatac
     1501 caggttagtg tcgagagact atgtgtcagt tggtaggcgg tggaagctgg aatcaggatc
     1561 atcgcgaaga cgaggatcgc accgacagtc ttgagggata tcaccactgt gagcgcgaca
     1621 agggtcagca agaggaaaaa tatgcgccgg gccggcactc cagaggcctc cgccatttct
     1681 tggtcgaatg cgatgaagta cagttctttc gagaagagca caatggcccc gatgacgacg
     1741 aaacttaatg ccgcaattgt gagtagttcc tcgctggtca cagcaagcac atttccaaag
     1801 aggtagccat agacctcggc gttataggtc ttcatcaggc cgatgaagag aatggccaaa
     1861 gccatagtgg cagtgtagag aatgccgatc gagacatcga gcttcatccg gcctttctct
     1921 tccatccatc ccgtagtcca gaccgtggca agaccaaaca gcaaggccat gagcagtgga
     1981 ggccacccca ttaaaaagcc cagcgcgacc ccggcaaacg ccgcatgtgc cgtaccggct
     2041 cccatgaacg cgagccctcg taacacgacg aagacaccga tcacagaaca caaccccccc
     2101 accatggccg ccgcgaggag ggagcgttgc atgaagtcgt aggcgaatag ttcaagcatt
     2161 gtagggcacg ttcaatgatg atggtgatag tcttcgacga tgacaagatc cttctctgta
     2221 atgacaagct ctttcccata gacctggctg aggatgtccg cctttaggac ttcggcagga
     2281 ggccctgcgg catagagacg agtcttgagg agcacaagcc ggttgacccg cgaacggatc
     2341 atattgatgt catgggtaat caagaggact gtcaaaccga gctcttcatg cagatgctgc
     2401 actaactcca cgacattgtg ctgcgtcgta atatccaatc cggtcgtcgg ctcgtccagc
     2461 aacaacacat ctggttgctg ggccaaggct ctggcgataa agacccgctg ttgttgtcct
     2521 ccagagaggt ggcctagtgc gctgtctcga tgcccttcca tgcccacatg agccagtgca
     2581 tctattgcga tctttcgatc ttttccagag ggtcggctga acacacctaa tgcgccgtac
     2641 cgtcccatca tcacggtttc caataccgtg acggggaagt tacggtcgat cactcccttc
     2701 tgcggcaggt agccgatcct cgcgcggtgg tgacagcgta attcatcaca ggcacaatcg
     2761 aaaatcctca agtggccctt cagtggagcc attaaaccga gcaccgcgcg gcagagagtc
     2821 gtctttcctg acccatttgg accgataacc cctacgaact cgcctgattg aatatcgagc
     2881 gagatatcct caagtgccac caccccaggg aacccaaagg tggcatgatc gaaatgaatg
     2941 atagggagcg actggcttgt catgtcggtg agggcccctc ttagtcctag ctgctgatac
     3001 tgatacctaa cgcacgctag gccgcgtcca gcgcgttgac caattggagc acattataac
     3061 ggagcatgtc gagatagctg tctgtatgcg ggagacctcc tgggagagtg gtgagcacca
     3121 ccacgcgcgc gctggtttcc ttggccaaca gctcgggagt tttttggctc agctgaattt
     3181 ccgagaccac gactttgatc tggtcttttt tgatcttcgc aatgagctcg tgaaggtgga
     3241 gcgccgatgg ttcactccct gattggggtt ggatcgtccc aacaatctga aagccaaatc
     3301 gtcttgcgaa atacggccag gctggatgat gagcgataaa tcgcctatcg ctcacgcgtg
     3361 tgatccgttc attcagttcc cctcgaagtt ggtccagttt ccgaagataa gaggcctgat
     3421 tggttcggta ttccgtcgca tgggccgggt ctacctgaat gagcgcttcc gtgatatgcc
     3481 gcatcatagt catcgcattc tcagggtcca accacacgtg aggatttcct ctctcatttt
     3541 cttcggcggc atgaggcgcg ccgtcatgct ctggtctgtc gcgaagcagt gcgatgcctt
     3601 tggacgtggt gacgacccgc aatgatgcgc tgccggcatt cttcactaac gaagagaccc
     3661 aaatttctag gccgataccg acttcaaaca gcagcttggc tttccgaact gcgacgagat
     3721 cactcggttt gggagagtag gtatgttcgt tttcgtaacc actcagtagg gaggtcaccc
     3781 gtacgtgaga gcctccgact tgttcggtca ggtcctttaa gaccggaatc gtgactacga
     3841 tattcagtgg ttccgcagaa gaggcagcga ggaccagggg gggggcgaac acaatgtggc
     3901 taacgaggat cacccagagg aggcgcacca attgaacatt caacatgggt gaggacgcta
     3961 acattgggtc ctttcgttgt caacgcaatg gaggggttga tcgaacggac gggatgctaa
     4021 aaaccacaac aatgggacac acattgtgtt tggtcacgcc tggcgatcct agaacgtcct
     4081 gtcctagctg ccccggccat acggacccgc aaagctggag caggccgaag tggtttttgt
     4141 cgtgtgagca ttcggtcacg acgtcttctc tgcttgaccc agtcggatag ttacattagc
     4201 gtagcgatcc tagagctcga ctagacgacc aatcggagga ggtaaggaat gcacgtcgtg
     4261 ggactcatgt ctggaacatc cggtgatggt gtcgatgcag cactcgtaga cattaccggg
     4321 cgaggtcgcg ctctgaaggc ccggacactt gcggcacaca ccttggccta tccacgatca
     4381 ttgcaacagc gcattctctc cgcgtcagtt tctgggacag tcgcagaggt ctgtcacctt
     4441 aatgcgctgc tcggtgaatg gttcgcgaac gcggcgctgc tcgtgattcg gcaagcgaac
     4501 ctccagccga gtgatgtggc gttgatcggc tctcatggac aaactgtgca tcaccttcca
     4561 catggcatcc atgcgccagg tgtcggcgcg attcgttcaa ccctgcagat cgccgagccg
     4621 gccgtgattg ccgagcgaac gggaattacc accgtggcca acttccgttc gcgcgacgtc
     4681 gccgcaggag gtcaaggagc tccgctggca cctgtcgcac atgcgttgct cttgaaacat
     4741 gcccgtcgcg cacggttggt tgtgaatctc gggggcatca gcaacgtgac gtatgtgccg
     4801 aaggggggac acctcattgg gatgcaggca ttcgatacgg ggccggccaa tatggtgctg
     4861 gatagtctga tggcgcgtgt gaccaatgga aggctattga tggatcgtga cggaaagcta
     4921 gccatgaaag ggcaggtcga ttcacgactc ttgggcaagc tgctcgcgca tccttttctg
     4981 tccaagcggc cacccaaatc gacggggcgg gaagcattcg gtcctgatgt catcgaggaa
     5041 gtgatcgcaa ttcagcagca acgcaggctt tcgatcgagg atctattggc gacctgcagt
     5101 atgtggacgg ccaaagcggt gggaacctcg cgacggtgga tcgaaggtga aatcgacgaa
     5161 gtagtggtag gcggtggtgg agtccgtaat cgtgcgatca tgagtcatct cgcaacagta
     5221 ttcgcaccga ttcctgtcac cacatttgag gccttggggt gggacagtaa ggtgtttgag
     5281 gccgtggcct ttgccctttt ggcctatcaa accgtgacgg ggcagtgggg caacatcccg
     5341 tccgtaacag gtgcgagcca tccggtactg cttggtacga tagtcccgaa cgggccgcgc
     5401 tggcgtgaat cccttcccgg gcgatagcga cacgatggag atcgtattca ttggccttgc
     5461 catcacgtgt ctggttttcc tggtttggcg gctgagagtc tcgtcaaaca ctgggctggc
     5521 cagaacagac gcctttgttc ttcctactgg tgtcacacta cgtccccagc ctctgctgac
     5581 tgatgcggat ctcttgctct acaatctcat acgtttagcc gtagaagacc attatctggt
     5641 ctttccgcgg gttccgctct ggtctgttgt cagcgtcgaa gcagaaggga cagttcgatc
     5701 gcaagtgttg agacagatcg cactgaagca actcgacttt gtgttagtcc acccaggaac
     5761 gaaggctgca gagcaggttg tgttgcttga ggacagttta ccttctcagc cacacgaggt
     5821 ctctcggagg cgggatatcg agtcagtctt acaggcagcg ggcatcgcat tgacgacctt
     5881 gaagccccat acatcctaca cagtccctca actggcccag ctgcttggcg tcagcgagga
     5941 cgagtgactg ccactgtgta ttatcgacgt atctcctgcc taatcttgat gtggaactag
     6001 tcagaggagc tgttaccggt caattgaggg gtcagttcct catcccgcat cggcgccgcg
     6061 tcgagagcct tccgggccaa ctctgcttca gcgctttcag gatattgatc gaccactcgc
     6121 tcatacatca tacgagcttt ttcgtgatcc ttcagcctcg tataggtttg cccgatcttt
     6181 aaggtagagg caacaagctt cggactgagt gggtagtagg acacaacgtg gtggaaggat
     6241 ttcaaggctt ccttgtaacg ccccaatcgg tactgacatt ctcccaccca atattgtgca
     6301 ttggctgcca gcgaggactg tccgtggagt tcgagaaaga atcgaaatcc agccaacgca
     6361 gcctcgtaat ccctgtgctt gaactcctgc atcacccgat catagagatg tcgagacgaa
     6421 tcctgcatgt tggaaggagc ggcgactgcg agattcacag aaaacagcac cgcaatcatt
     6481 cctggaatca cgatcatcag agccttcatg atcatacctc cgtactgctc ggcaaggtcc
     6541 cggtaaccat cctcccgtaa tgtcgcaatc gccgtgccac cacgagatga tctggtgtca
     6601 ctaaaagagc caagaatcca ggaccttggc atcattacac acagggaaat gccgagtgac
     6661 cgaccgggcc tgagcgacag atttgtacga tctgtgcggg agtccccccg cttgacagaa
     6721 tggagcacac cagccgagaa atggcttgag tcaatgcttt gcagagaact tgacttgcct
     6781 gctcgtggtc gaaataatgc ccacggccac tgaagttaga gtctcaagaa tagaacgatg
     6841 acgctgaaag gaagaaaagc gtggcgaagg gagctggaca tgaggattga gcctccgccg
     6901 aatccactcg gcggaaagac cctgatcgtc gacgcgaatg acggcgactc atacgtacga
     6961 cctagtgcag cactcctcga tgcgggggag catgaccagg tgtttgttcg tccaggaatc
     7021 tatgaggaca aagtctttat cactgggcga ccgattcatt tggtaggggc cggtcgagac
     7081 gaggtacaga ttttctcccg ccgtggaggc cccctctatc ttcagcaagt cccaagcggt
     7141 cgaatctccg gtatcacgtt tcgatacgtc ggaagcgacc agaattcagc gatgaacctg
     7201 ctcgattctt cttgtactgt gacacagtgc cgcgccatgg aaggcattct gtctggtgtg
     7261 gtgatttatg gaccgcaatc tcggctaaca tttgtcgaga atgaagtttg tggcaaccgt
     7321 gaatcgggaa tttttgtatt tgccggggca cagccaagga tcgccgacaa tctgtgtcgg
     7381 agcaatcacc atttcggcat tgcggtgcgg gatcctggaa gccatccgga actcgtcagg
     7441 aaccgatgcc gggaaaatat gttgagcgga atcctcctct ttcatcacgc ggaagcgctc
     7501 ctcgttgaca atacgtgcag cgagaaccag cactggggga tggtgatgac tcccgactgc
     7561 caccctactc ccaatcgaga ggcgctcgac acttcaaaca ttcttgctcc caatccgcgc
     7621 ggaactttga tcgtcacgga tcaaccgtta aatgacatcg gacgctgaat agacaggtat
     7681 cgaggacacc ggtaatccgg aatcggctcg tcatgtactg tgggcgaggt cgaagaggag
     7741 tcagaagccg tctaaggcgc gcgatgcacg ttatggtagg aggtttgccg aatgggttcc
     7801 agaggcttca tgaaggtgga aggcactgcc accttgcttt tttgctcgat acatagcggt
     7861 gtcggcatgc ttgagtaatt cgtcgaccgg gtgatcgtcg tgaggataga ttgtaattcc
     7921 aatgctgacg ccgacagaga tgtggtggcc ctgtaactca aatatcgtgg caatggactc
     7981 cgtgattcgt tttgccacca caagtatatt ctcatcggat gagacacctt ctagaatgat
     8041 ggtgaactca tccccgccca tacgggccac ggtatcgact tcccgcacac aggtcttgag
     8101 tcgttcagat acggctttga gtagttcgtc cccgatgtca tggccatagg tatcattcac
     8161 ggctttgaat cgatcgagat cgaggagcat gagaccaatc ggttgatgca tgcgtttgct
     8221 tcgagccatg gcttgcacca gccgatcacg aaagagggta cgattcacca gcccagtcaa
     8281 gtgatcgtac tgtgcaaggt aggtcagacg ttcttcagtg cgtttccgct caatagcgta
     8341 gcggacagca cgagctaaca gctcgggatg accctggcct ttgacgagat aatcctgcgc
     8401 cccctgttgg accgcttgaa gagccagact ttggtcgctc acgccgctca ggacgacaac
     8461 ggggatcgtg ggactagtat cgtgaatctg tctgaccgtc gggagaccga acgcatccgg
     8521 gagtgacaga tccaagagca cggcatcgaa tcgctcacgg ctcagatacg tgagcccttc
     8581 tcctaacgtt ttcacacgac tcacctggaa ctgctcgcta ctccactcag aaagaagatc
     8641 ctgcgtaagc tgagcgtcga catcattgtc ttcgatgaga aggactttaa tcatctggca
     8701 tccttgcgat agccgaactc ctggttgtgg ttcttaattt ccaagtctgg accggttgtc
     8761 tgtgacaaga ccactactat gagtggctaa ttatagcgaa gagacagggg aggataaagc
     8821 aaatgcaaca atacggaaca cgaacgctgg aaacctttct tcgaggttca cgccagggac
     8881 gggcctgaga gcataggcac acatgaccat cctccccagt gaaaccatga ccgccccttg
     8941 taagtcgaat acaagtttcg actacgtagg gcggtcatga ggaatctggc ggacaatccg
     9001 gaatgaaggc tgcctaatat gagcctacat ggagtgcttc agctcggcga cgaacatctt
     9061 cggctttttg cgattgcccg aggttgtcat agagtgaggc gatattcgca tgcagatgtg
     9121 acaggagttg ccccaactgt gcttcttgtg cgattacatt cgcttcgagt gccagtttct
     9181 tttcctcact ccggcggaag cgttcctcaa gccgctgtac aagttggatc caacctttga
     9241 ccttcccgga atctacgtca gggagggtcg gcaaggcttc ggcacgatcg agagattttt
     9301 cagtttgatt catccaattg atgaagacga gaaaatcacc gagcgttatc ttcagtgcgg
     9361 atgaggcgcc ttcttcttcg ataaccaggt cttccattgc aactccttgt gaagcgaata
     9421 atttctgatt gtgtacggca gacgagcgaa tatacttctg ccaaattgcc gtcgaagctt
     9481 gctgttaccg gcttctgaaa aatgaacaac gaagtgagat cgatgcgagc cacctccttt
     9541 ctaagcctgg cctttggcga aaaacgtaga gctggttcaa gagagggcaa cccatcggct
     9601 acatctggta gtagttggcg agtaatactg tgccagcagt agtattgtca agaggcctct
     9661 gaggggattg gtcaagagaa aactctcgcg gattcttcgg tatgggggca acgtcaagaa
     9721 gaacaggaat tagacaaaga ttatccttgt ggtgagaggg gcggaactcc tggatagatt
     9781 cctcgcggga gacgttcgga gccaattgtc ccatcgcagg cataccctgg ctcaaaaggc
     9841 cagaggagcg accgaccttg ccaagaacct ctcacctgga ccatggccag cgagatccca
     9901 agtcgagccg cctcatcgcg cattgtcgag aggcagcctt gataggtata tgcgcggcga
     9961 cccagtaaga tcggctcgcc tgtctggcta tggaagaccg tataggactc ggcgcagccg
    10021 gccaggagca gtaccatgag gagcaaaaga cctctcgtca tgtgcctcca ccctcaccca
    10081 ttggtgcgat cttcatcaag attgtgtccc tttccacgca ctggatcgag atggtagtgc
    10141 gagagcgacc tcatgtttga caagccgacc taagcggact tggtcttcac ctggcatttg
    10201 gataaattca acaccgaacc gtcgtccctc gcaccagcgc actttggcta gtccgactcg
    10261 gagcggagac atggtgtcag gaacgagcat tctcatttcg aggtaggaac cgggctgtac
    10321 acgatgtcgg ctgtggacgg cgcaaccagg gacagacacg ttcaacactg ttccttcccc
    10381 cacataggcc tcaccagcaa acaccacagg gaactgcgca tggatacgtt cgcagtacct
    10441 cagtttcgtc atggtggtcg tctccgtctc ggtcggataa ttggcaaagc aaagcgtacc
    10501 acagtcttcc tttagcacgc gagagggccc cccttatgag atgtggcaca agagacattg
    10561 agggactatt gcagtgtctt ccggcaaccg caaagtgcgg caatcagagc caagaaaagg
    10621 aaaggctctc cctcgtatcc ctacgaaagt tgagagccgt gaataaccat aaggtattga
    10681 aatactagca caataaatat tcatctcttc cccgtcattc ggcatagaac ctgcgcttac
    10741 atcgtcacag aacagggtca acgtccgaac agtatgaacg attgtccaca agaaatatcg
    10801 acacagggaa gtagaaaaca aagatgaaag tcaagctacc caacaaacta tctagcgatg
    10861 acacaggatg ccgggcactc agacctatgg gttccgccag atatcttgcc tgcgcagtcg
    10921 tgataatgat ggcgactacg acgcccgttg ttctccccga tacgctggtc tatggatata
    10981 ccagtcccac gggtctggta aaccttacga atgtaccgac agatgtgcgt gtcccagcaa
    11041 gtcgccgtaa ggcacggtat cacatgggtg taggggatct cgaactcgag caagcagtcc
    11101 gtcgtgcggc tcagcagcac cgtgttcaac cggccttgct tcttgctgtg atgagggctg
    11161 agtcttcctt taatccaaca gcaatctcaa aggcgggagc agtgggactc atgcaactga
    11221 ttccggaaac cgccatccgg catggcgtgc ggaacctcta cgatgcgaat gagaacatcg
    11281 ccggtggggc gaagcacctg cgttacctct tagatcggtt ccacggaaac gtccgattag
    11341 ccgtggctgc ctataacgcg ggtgaacgca aggtggatcg atatggacag atcccaccct
    11401 ataaggaaac acaagactat gtaaaaaaag tgctcgtcta ctaccgtagc tacaagaagg
    11461 acggttggat catgccggtt gtgacagcca ccccgctaac gcacgtaggt cgaccgtact
    11521 aatcgatcgc ctctgtagcc atgaggcagc actgttcaga aagtcaaact acaatcagcg
    11581 agtggaggaa agggaggcat tagacaggct tttcagcggc gtattccaaa tacttccaga
    11641 gttccttagc ctccgcttcg gcaagcacca gcggagtgtt gttcgcggat ccgacgaaat
    11701 gaacttttag cgacatctcg tcttgccaga tctgtgcttc gcagtagctc acactatcga
    11761 ggtttaatgc cttcttgcca atgcgaacaa agtgcatcgc gtctccttgc tctgcgccaa
    11821 ctcctcgccg actatagcac cacatggctt tccgtcaaat gcttaccgca ttttatcgca
    11881 aggtctcgca ggcacgtcag cacttgatga gggctaacac tcctggagat agattaactg
    11941 aagagaaacg gttggttgcg gctgagggat ttactcgtca tctaacctcg aaaacattta
    12001 ggattgcgct gcgttaggca ttttctgcgc gactgcatca gatcgctgcc accttgtcat
    12061 aatactgatg atcttctctg tagcctccgt gaccttctcc gatcgtggca tacgtatcga
    12121 ctacctcaat cgtacgaaga tacttcacca tcttatatcc cgttttcgtt tcaatgcgca
    12181 ggcgacaggg cgcaccatgc ggaatcggca gaggcctcca gttcatttca tacgccagaa
    12241 tggtctgcgg gtcgaggact tcatcgagtg tcaagatctc gtaatacgtg actggcgcat
    12301 actccgg
//
LOCUS       DATIPF010000170         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_737876, whole genome shotgun sequence.
ACCESSION   DATIPF010000170 DATIPF010000000
VERSION     DATIPF010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737876"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VLL06_04460"
     CDS             <1..566
                     /locus_tag="VLL06_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30250.1"
                     /translation="LKKLRATLEHIDASRNSGNLENSGRDSHAIREMEGSIERDFQAP
                     KLSTGPKTGSSIGNAPAAGPSIGKTPKTGNTIGAEGVTGQNIGSSSKNSHDIGGSGPV
                     GFGIGTTGRAGPSIGESTFNSESSSAVGSSLQRSTVGSNLSDSTVGSSYGGSNVGSSL
                     GESGVGSSFGSSSVGSTIQGRGTAPRQ"
     gene            complement(573..1592)
                     /locus_tag="VLL06_04465"
     CDS             complement(573..1592)
                     /locus_tag="VLL06_04465"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30251.1"
                     /translation="MTYALAFLLLLATALAGCNRGDPIVVIQLNPKNPDIIYVATNDY
                     IYKTRDGGQTWTNLSHGMSHSRVISMAIDPAYPATVYAGTKGDAVFKSHDGGQHWTSM
                     RSGLDDATISSVVNQFLFDPTDSNHIFLATTMGVSETKDGGEHWVKKMEGMKEVLMVV
                     TLGMDPTRPAVLYAGTSGGVYKTIDEAGHWEKVNNGLVSPDIIKSSRALSVTSVLVDP
                     FEPETVYASTLAGLYKTTDGGKAWVRIGQLLQDQMLFSMILDRTKRGVIYLGGREGVH
                     RSEDGGATWSTLNSGLATLNIRSLVQSPSDPDVFYAGTNGSGLYRSKNRGETWEVVPT
                     ALSKQ"
     gene            complement(1744..>2089)
                     /locus_tag="VLL06_04470"
     CDS             complement(1744..>2089)
                     /locus_tag="VLL06_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HSS30252.1"
                     /translation="VLVSDDEYKLNAVNDIVKTKCVKRGVSLKAFTYGTVEPALSGTV
                     RQVAKIQSGIAADKAKEVTKAIKESKLKVQAQIQGEQVRVLSKSKDDLQSAMAFLRSK
                     DFGIDLQFTNYR"
BASE COUNT          461 a          644 c          522 g          462 t
ORIGIN      
        1 atctcaagaa acttcgcgcg acgctcgaac acatcgacgc ctctcgcaat tcaggaaacc
       61 ttgaaaactc aggtcgggat tcgcacgcga tccgcgaaat ggaaggatcg attgagagag
      121 acttccaagc accgaagctc tcgactgggc caaagacggg ctccagtatc ggcaatgcac
      181 cggcagccgg accgagtatt ggaaaaaccc caaagaccgg caatacgatt ggcgcggaag
      241 gcgtaaccgg ccaaaacata gggtcgtcat cgaaaaacag tcacgacatc ggcggctcag
      301 gcccagtagg gtttgggatc ggcaccacag gccgtgcagg tccttcaatc ggtgagtcca
      361 ccttcaacag cgaatcgtct tcggcggtcg gctcctcgct gcaacgttcc accgtgggat
      421 cgaatctgtc ggattcaacg gtcggatcga gctacggtgg ctctaacgtg ggctcaagcc
      481 tcggagagtc tggtgttgga tcgtcattcg gatcgtcttc ggtcggttcg accatccaag
      541 gacgcggcac cgccccgcgg cagtaatctc cttcactgct ttgaaagcgc agtcggtacc
      601 acttcccagg tctctccacg atttttgctc ctatacaagc cactcccatt tgtccctgca
      661 taaaacacat ccggatcgct cggactctgc acgagtgatc tgatattaag cgttgcaaga
      721 cccgagttca gagtgctcca cgtcgcccca ccatcttcgc tccgatggac gccttcccta
      781 ccgcctagat aaatgacgcc cctcttcgtg cggtccaaga tcatcgaaaa caacatctga
      841 tcttggagca actgtccgat ccgtacccag gccttcccgc catccgtcgt cttatagagt
      901 cctgccagtg tggaggcgta caccgtttcc ggctcaaacg ggtcgaccag caccgacgtc
      961 acgctgagcg cccgcgacga cttaataatg tcgggcgaca cgagtccgtt gttcaccttc
     1021 tcccagtgac ccgcctcgtc gatcgtttta tacactccgc cactcgtacc cgcatacaag
     1081 accgccggcc tcgtcggatc catccccagc gtgaccacca tcagcacttc tttcatccct
     1141 tccattttct tcacccaatg ctcgccgccg tctttcgtct cggagacacc catcgtcgtg
     1201 gcaagaaaga tgtggttgct gtctgtcgga tcgaaaagga attgattcac caccgacgag
     1261 atcgtggcat cgtctaaccc cgaacgcatc gaggtccagt gctgtccgcc atcgtgactc
     1321 ttaaagacag catcaccctt cgtacccgca tacaccgtgg ccggataggc tggatcgatc
     1381 gccatagaaa tcacgcgcga gtggctcatc ccatgtgaca gattcgtcca cgtctgaccc
     1441 ccatcgcgcg tcttatagat gtagtcgttc gtcgcgacgt agatgatgtc aggattcttc
     1501 ggattgagct ggataacaac aatgggatcg ccgcgattgc agcccgcgag agccgtagcg
     1561 agaagaagca gaaaagcaag agcgtatgtc atgagttgga tattgcagca tctatcccga
     1621 atgctcaaac aggtcgtcca acaaggccgc agggagaatc gaacaccgag gcgtgctgct
     1681 gctgcacgtt gaggtgttcg atcgactgag aacgaagttg gaggcctgtt tcagcatccg
     1741 gggctagcgg tagttggtga actgcagatc aattccaaaa tccttgctcc tcagaaacgc
     1801 catagccgat tggagatcat ccttgctctt gctcagcacg cgcacttgtt caccttgaat
     1861 ctgcgcctgt actttcagct tggactcctt gatggccttc gtgacctctt ttgccttgtc
     1921 cgccgcgatc ccgctttgaa tctttgcgac ctggcgcacc gtcccactca gtgccggctc
     1981 cacggttcca taggtaaacg ctttcagcga gaccccgcgc ttcacgcact tggtcttcac
     2041 aatatcgttc accgcattga gcttatactc atcgtcggat accaggacc
//
LOCUS       DATIPF010000171         3328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1447084, whole genome shotgun sequence.
ACCESSION   DATIPF010000171 DATIPF010000000
VERSION     DATIPF010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447084"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VLL06_04475"
     CDS             complement(<1..438)
                     /locus_tag="VLL06_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HSS30253.1"
                     /translation="MSTLDRLLGLLRYLKPYRVRLGAAFVCSGLVAAFSGAYAWLVKP
                     VLDEIFINKNESLLLVLPIALFVVAVLKSVFNYGQNYLMSYVGNRVITDIRQELFGTL
                     IRLPVPYHDGNTSGRLVSRVVNDVTLMANAVAGVLKDLAEVTTA"
     gene            complement(426..1553)
                     /gene="lpxB"
                     /locus_tag="VLL06_04480"
     CDS             complement(426..1553)
                     /gene="lpxB"
                     /locus_tag="VLL06_04480"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HSS30254.1"
                     /translation="MRILIVTGEASGDLHGAHLAKAIMALDPTTELVGIGGPSMRAAG
                     VNLVPGVPQLDVMGLIGLSAIRAMLQRVRAIRRVLKAEAWDLVVLIDNPGLNFHFARV
                     AKAAGRRVLYYIAPQLWAWRPGRMKWIQRRVDHVVVILPFEPELYHRAGVRCTFVGHP
                     LLDMVAEHYDRAKLRREFGLSESARVVGLLPGSRVSEVEMLLPVLLKTAAQLVLAEPG
                     TQFILAQASSIDDNLIQTLLRDSPVPVRVVHDRASEVMALSDVLLIASGTATLQAAVV
                     GTPMVLLYKTSPLTYRLARWLITVKWIGLVNLVGGRLIVPELVQEDATDERLCREVLH
                     LLRDPSAYNDMKEGLKQVRQSLGEPGASSRAAQVVLSECRL"
     gene            complement(1641..2654)
                     /locus_tag="VLL06_04485"
     CDS             complement(1641..2654)
                     /locus_tag="VLL06_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSS30255.1"
                     /translation="MESKAQRMTPFRVGVIGVGHLGQHHARLYASLPGAQLVGVADQS
                     LERAQMIATRHGARVFRTADELLPHVDAVSVAIPTSGHYAVAKACLQAGKHVLVEKPI
                     AVTPEDAHELVQLAKQRGCCLQVGHSERFNPVMALMRPHIGRPIFIECHRLSSFSERG
                     TDVDVVLDLMVHDLDLVLSLHPGPVEEVRAAGVAVLSSSIDIANARIQFRSGCVANLT
                     SSRVSTNKMRRLRLFQRENYLSIDFQTRQGMICRRTAKAGERPTVEVEQYQGGDEEPL
                     KLQLESFLHAAATGIRPVVSGEDGAAAVDVAHQVLQAIAAFSARHEEGDSWEKKIVGV
                     AGS"
     gene            complement(2644..>3328)
                     /gene="lpxI"
                     /locus_tag="VLL06_04490"
     CDS             complement(2644..>3328)
                     /gene="lpxI"
                     /locus_tag="VLL06_04490"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249556.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HSS30256.1"
                     /translation="HWIKIGQLSKLIKAFKEDRVHQAVMLGGIKKTHVFTTVRPDFRT
                     LAMATRLALWKDDDILRAFAKELEQEGIAICESTFGLEGILVEEGSLTACTPTEKEWE
                     DIRYGWDVAHDIGRLDIGQCVVIKDRVVVAVEAVEGTDGAIKRGGELAKEGAVVVKRS
                     KPQQDLRFDLPAIGPRTIEVMASVKASVLAVEAGRTILLDREIMLDKARSARIAIVGI
                     AKVEPSGGK"
BASE COUNT          714 a         1087 c          869 g          658 t
ORIGIN      
        1 cgcggtggtc acctcggcga ggtctttgag tacccccgcc accgcgttgg ccatcaatgt
       61 gacatcgttc acgacacggg acaccaaccg ccccgacgta ttgccatcgt gatagggaac
      121 gggcagccgg atgagtgttc caaataactc ctgccgaatg tcagtgatca cccgattccc
      181 gacatagctc atgaggtagt tttgcccgta gttgaagacg ctcttgagca ccgccacgac
      241 gaagagcgcg atcggaagca ccagcaagag gctttcgttt ttgttgataa aaatctcgtc
      301 gagaaccggc ttgacgagcc aggcataggc gccggagaag gccgccacca accccgagca
      361 cacaaacgcc gcacccaatc gaacccggta gggcttcagg tagcggagca atcctaacaa
      421 gcgatctaga gtcgacattc ggacaatacc acctgtgccg ctcggctaga ggcgccgggc
      481 tctcccaatg attgtcgaac ctgtttcagc ccttctttca tatcattata tgccgaagga
      541 tcgcgcagca ggtgtaagac ttcccggcac aaccgctcat ccgtcgcgtc ctcctgaacc
      601 agctccggta cgatcagtcg gcctcccacc aaattgacga gcccgatcca ttttaccgtg
      661 atcagccaac gagccagccg ataagtcaac ggcgacgtct tgtatagtaa caccatgggc
      721 gtccccacca ccgccgcctg caaggttgca gtccccgatg caatcaatag cacatcggag
      781 agcgccatca cctcactggc tcgatcgtgc accactcgga ccggcacggg gctgtcccgg
      841 agcagggtct ggatcagatt atcgtcaatt gaggaggcct gcgccaggat aaactgcgtt
      901 cccggttcgg ccaggaccag ttgagccgcc gtcttgagga ggaccggcag gagcatttcg
      961 acttcgctca cgcggctccc cggcaacaat ccgacaacgc gggcggactc gctcaggccg
     1021 aattctctgc gaagcttggc gcgatcataa tgctccgcca ccatgtcaag caatggatgg
     1081 ccgacgaacg tgcaccgaac accggcccgg tgataaagct ccggctcgaa tgggagaatg
     1141 accacgacat ggtcgacccg gcgctgaatc cacttcatgc ggcccggccg ccaagcccat
     1201 agttgtggcg caatgtaata gagcacgcgt cggccagcgg ctttggcaac ccgggcgaaa
     1261 tgaaagttca accctggatt gtcgatcagc accacaagat cccaggcctc tgctttcagc
     1321 acccgccgaa tcgcgcgtac gcgttggagc atggctcgga tagcggacag cccgatcaat
     1381 cccatcacgt ccagttgcgg gacgcctggc actagattca caccggccgc gcgcatcgaa
     1441 ggcccaccaa tacccaccag ctcggtcgtc gggtcgagcg ccatgatcgc tttggctaaa
     1501 tgggccccat ggagatcacc cgaggcttcg cctgtgacga tcagaattcg catacccttg
     1561 ggaacggggt aggggtacat ccttaaacca tctatcccgt taccccgatc ttccctttat
     1621 ctatcctaat cctacgaccc tcatgaccct gcgaccccta caatcttttt ctcccaacta
     1681 tccccttctt catggcgagc gctaaaggcc gcgatggcct gcaagacctg atgtgccaca
     1741 tctactgccg ctgcgccatc ctcacccgat accaccggcc ttatgcccgt agcggctgcg
     1801 tgaagaaacg attcaagttg gagcttgagc ggttcttcgt ctccaccctg atactgctca
     1861 acctcgacgg tcggccgctc cccggccttc gccgtacgtc ggcaaatcat cccctgtcga
     1921 gtctggaaat cgattgacag atagttctct cgctggaaga ggcgcaggcg gcgcatctta
     1981 ttggtcgaca cacgactgga ggtcaggttg gccacgcaac cgcttcggaa ctgaatgcgg
     2041 gcattggcga tatcgattga ggaggacaac accgccaccc ccgccgcgcg cacctcttca
     2101 acgggcccag gatgcaagga caggacgagg tccagatcgt gaaccatcaa gtctaacacc
     2161 acatccacgt cggtcccccg ttcactgaag ctgctgagac ggtgacattc gataaagata
     2221 ggccgtccga tatggggacg catcagggcc atcaccggat tgaaccgttc actatgaccc
     2281 acctgcaagc aacacccccg ctgcttcgcc aattgcacca actcgtgcgc gtcttccggc
     2341 gttacagcaa tcggcttttc aaccaggaca tgtttgcccg cctgaagaca ggcctttgcg
     2401 acggcatagt ggcctgacgt agggatggcc acactgaccg catcgacgtg cggcaacaat
     2461 tcatcggccg tgcgaaagac tctggcccca tgccgggttg cgatcatctg cgcccgctcg
     2521 agcgactgat cggccacgcc gacgagctga gctcccggca aggaggcata gagacgcgca
     2581 tggtgctgcc cgagatgtcc cacaccgatg acgcctaccc taaacggtgt cattcgttgg
     2641 gccttacttt ccaccactcg gctcgacttt tgcgatgccg acgatggcaa ttctggcgga
     2701 ccgggccttg tccaacatga tctcgcgatc caagagtatt gttcgaccgg cttccacggc
     2761 caataccgag gccttgaccg acgccatcac ctcgatggtc ctgggaccga tggcagggag
     2821 atcaaaacgc aaatcctgtt gtggcttcga tcgtttcaca accaccgcgc cttctttggc
     2881 caattcgccg ccccgcttga tggccccgtc ggtcccttcg accgcttcaa ctgctacgac
     2941 cacgcgatcc ttgatcacaa cgcattgccc gatatcgagt cgaccgatgt catgcgccac
     3001 gtcccatccg taacggatat cttcccactc tttttccgtg ggggtgcacg cagtcaacga
     3061 cccctcttcc accaggatcc cttcgagccc gaaggtcgat tcgcagatcg cgatgccttc
     3121 ctgctcaagc tccttcgcga atgcgcgcag aatgtcgtca tccttccaaa gcgccaagcg
     3181 cgtcgccatc gccagagtgc gaaagtccgg tcgtacggtg gtaaaaacgt gcgtcttctt
     3241 gatgccgccc agcatgaccg cctgatggac tcggtcttcc ttgaaggcct taatcagctt
     3301 gctaagctgg ccgatcttga tccaatgg
//
LOCUS       DATIPF010000172         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_829926, whole genome shotgun sequence.
ACCESSION   DATIPF010000172 DATIPF010000000
VERSION     DATIPF010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829926"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            376..861
                     /locus_tag="VLL06_04495"
     CDS             376..861
                     /locus_tag="VLL06_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30257.1"
                     /translation="MLAMRLVRLIEGHSEQLSRDLSEKVWNSPRCSDLRKVPANELEA
                     RTREIYRNLSDWLVGKTEADVERRYTELGATRAQQGVAYSHFVWAITATREHMRDFVQ
                     REGLSDSAMELHGELELMHLLGQFFDRALYYAAVGYERERDHIGGKQRRRKGDDPRKP
                     A"
     gene            complement(1181..2152)
                     /locus_tag="VLL06_04500"
     CDS             complement(1181..2152)
                     /locus_tag="VLL06_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HSS30258.1"
                     /translation="MPEQSVSQPVTSSLDSLTLALVVSGMGVCTDEAVTGLRRDVESK
                     AVTHSSLEFDASLARAKRHFFESEAHFFLCDGEPCRQRRGFEITSHALQSEAERIGCR
                     ISPTACQGPCKQAPLALLRVGHGCELFSQFARRREWEAVLSFAQRAVQAKTLLVDAGT
                     AQPFRFDPIHEPEKPSAALEYVRFLLGHFEGTVELPQEQRSIQKEVVGSWEAGGRFLS
                     LRMAATYRKANGSWDRHQAFIVLGPDEETGALVSRAYTDGGKIHEFHLVMEDGRLMFP
                     DRMPHNMPAVAARKVLAPTAQGYEERVEIDWGRGVYEPYYSMPLQRK"
BASE COUNT          499 a          691 c          650 g          528 t
ORIGIN      
        1 taggaaggat catcgatagc catgggtgcc atttttgctc gccaagttcc cacgataagg
       61 ctgtggtcgt tcgatgtgca cgatgaagaa cataccagct gccctccggt ttgatccacg
      121 tcgtttctga aggatcgttc gtctctcttg gctgaagcga ccagccatca acgacttcaa
      181 ttgcatctcg tcttttacta aaagccggaa acgttgctca gtataattgg gcgcatattt
      241 ccgatgcgac cagcaccagc tggctttgac catcacgtga ttgacgttgg tgccatcgat
      301 ctagtaaggt ctcccccgga attaggggtt tgcgaattct cctacgcagg cgcatgttgt
      361 atctggaggt aggctatgct ggctatgcga ttggtgcggc tcatcgaggg tcattccgaa
      421 cagctttccc gcgacctcag cgagaaggtg tggaactctc cccggtgtag cgatctccgc
      481 aaagttccgg cgaacgaact cgaagcccgc acccgcgaga tctatcgcaa tctgagtgac
      541 tggctggtgg gcaagaccga ggccgatgtg gagcggcgtt atacagagct gggcgcgacg
      601 cgagcccagc agggcgtggc ctacagccac ttcgtctggg ccatcaccgc caccagggaa
      661 catatgcgcg acttcgtcca gcgcgaaggt ctgtccgata gcgctatgga attgcatggg
      721 gagctggagc tgatgcacct gctcggccaa ttcttcgatc gtgcgctgta ctatgctgcc
      781 gtgggttatg agcgcgagcg cgaccacata ggagggaagc aacggaggcg caagggagat
      841 gacccgagaa aacccgcgta gtgccctatc ggctgggaac tcgtgccttt cgcaatagcc
      901 acggagtaaa cggatgagtg tcaacctgta atcctattct cacctctcgt gcctcgactt
      961 tcagccgctc tcgcgtccta ctccctctca tatacgtctg ataccacgtg aaattggata
     1021 tcccgcggca atgaatcttg ttgaagagac cacggactcc attgacggca atcgtcctgc
     1081 ctgtccgatc gcccatgagc cgaattaaaa agccactcaa gaaaatgaat cgggagtctt
     1141 gttcatcagt tgattttctc caggctcccg cgtgatgtga tcattttctc tgaaggggca
     1201 tactgtaata gggttcgtag accccacgtc cccagtcgat ttcaacccgc tcctcatatc
     1261 cctgtgcggt aggggccagt acttttctgg cggccacggc aggcatgttg tgcggcatgc
     1321 gatccggaaa catgagccgt ccgtcctcca tgacgagatg gaactcgtgg atctttcccc
     1381 cgtccgtata cgctcggctc acaagtgcac cggtctcctc gtccggaccg aggacaataa
     1441 aggcctgatg ccgatcccag gacccattgg cctttcgata ggtggctgcc atccgaagac
     1501 ttagaaagcg cccgcctgcc tcccaacttc cgaccacttc cttctggatg gatcgttgct
     1561 cctgaggcaa ctccaccgtt ccttcgaaat gtcccaggag aaaccggacg tattcgagcg
     1621 ccgcactggg tttttccggt tcatgaatgg gatcgaaccg aaagggctgc gccgtccctg
     1681 catcgacgag caacgtcttc gcctgaaccg ctcgttgtgc aaaactcaag acagcttccc
     1741 actctctgcg gcgtgcgaac tgggaaaaca attcacaccc gtgaccgaca cgcagcaatg
     1801 ccagcggcgc ttgcttgcag ggcccttggc aggccgttgg tgaaatccga cagccaatgc
     1861 gttcagcttc agactgcaac gcgtgcgagg taatctcgaa ccctcttcgc tgccggcagg
     1921 gctctccatc gcagagaaag aagtgggcct cactttcgaa aaagtgccgt ttcgcacggg
     1981 ccagagaggc atcgaattcc agcgagctgt gtgtgaccgc tttcgactca acatcgcggc
     2041 gaagacctgt aaccgcctcg tccgtgcaga cacccatccc gctgacgacc agtgcaaggg
     2101 tgagactatc cagcgacgag gtgacgggct gactcactga ctgctctggc ataggtcctc
     2161 cttctgcacc ggacgtggca tgttcgactg tttgcatcgc ggcttcaggt catcagcggc
     2221 cggctcgacc atgtaaatca tgagtgcgtt gttcacggga gagggccgac atgcgccacg
     2281 atcctcaaaa cggttgcgta ttgccgaagc gaaccgacat tcacgcggct tgcgtgtgtg
     2341 cttagtctgg cttcatgtgc cgttctaa
//
LOCUS       DATIPF010000173         3028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1536805, whole genome shotgun sequence.
ACCESSION   DATIPF010000173 DATIPF010000000
VERSION     DATIPF010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536805"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VLL06_04505"
     CDS             <1..763
                     /locus_tag="VLL06_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HSS30259.1"
                     /translation="LTVSSGVAPYQTTLHSSPIASHQTLAGQIIRQRRSAVSFDGTTS
                     ISAATFFHMMQRVMPRSDLPQMDRPMPWDVWPHKPRIHLVLFVHRVDGLTPGLYVLVR
                     DPQRLSFIQQSMNPELTWTLAPGCPEDLPLYWLLEGDARRAAVQVSCHQDIAGDSAFS
                     LGMLAEFEGCLRKGGAWWYPRLFWESGLLGQVLYLEAEAAGVRATGIGCFFDDPVHEI
                     VGIKEYSLQSLYHFTVGGPVDDTRLMTLPAYHHLV"
     gene            812..1666
                     /locus_tag="VLL06_04510"
     CDS             812..1666
                     /locus_tag="VLL06_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30260.1"
                     /translation="MFKIKKKSGKPKATILYNVVEDYSEFDAPGNVTVCAMTYKEDLI
                     AHAKVLSESYDIPLETMTRLLTEGGVYVYPQVGGVLTRGLFACRIDPTGEIPKQTWTL
                     DLMQLAEAEQLAQARGIPLEEVAALVFHWTLPEELRTKLEQKNLGIDYRTLDRAVAKG
                     GDIKYIDFRKDWSPHFKRLCIMPDGRLVETGGLEEFAQLHGITSAQARTLVEQGGTLE
                     VNGELLACQIVNGQPAVARFSAKNYSKAKELAKTKGLHVMDALSEVGYNDPAMMRGLV
                     RKELSVRS"
     gene            complement(1703..2065)
                     /locus_tag="VLL06_04515"
     CDS             complement(1703..2065)
                     /locus_tag="VLL06_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS30261.1"
                     /translation="MATILVIDDEESIRNLLKEVLERAKHHVLEASDGREGLRLYQQN
                     KVDLVLMDILMPGTDGLEATLQLTREYLDAKVIAMTGAQGERNFLDVAKLFGARRVFE
                     KPFNLDKLVEAINQELAK"
     gene            2315..2881
                     /locus_tag="VLL06_04520"
     CDS             2315..2881
                     /locus_tag="VLL06_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HSS30262.1"
                     /translation="MQDPDSWSWTRRSLLKTSVVGLLLLGSRLLSPSLARANELPDGE
                     LTFFNVRTNERLRVRYRDDAGNYDLMALDEVNHILRCHHTGEVAAIDVRMLEHVNLVQ
                     NTVGGGNEIHVISGYRSPEYNAQLVKRSRRVAQHSLHVQGQALDFYIPGVKVRELRHA
                     ALKLHYGGVGVYPRAKFIHLDCGPFRTW"
BASE COUNT          655 a          756 c          901 g          716 t
ORIGIN      
        1 gctgacggtt tcgtcgggag tcgcgcccta tcagacaacc cttcactcct cacccattgc
       61 ctctcaccag accttagccg gacagatcat tcgccagcgg cgcagcgccg tgtcgttcga
      121 tggcacgaca tcaatctcag cggcgacgtt tttccatatg atgcagcggg tgatgcctcg
      181 ttcggatctg ccgcaaatgg atcgaccgat gccgtgggac gtctggcccc ataagcctag
      241 gattcatctc gtgctgttcg tgcaccgcgt cgacggcctc acaccagggc tgtatgttct
      301 ggtccgtgat ccacagaggc tgtcgttcat ccaacaatcc atgaatcccg agctgacctg
      361 gacgttggcc cctggttgtc ctgaggatct gcctctttac tggctgcttg aaggcgatgc
      421 aaggagagcc gcggtgcaag tgagttgcca tcaagatatc gcgggggaca gcgccttctc
      481 gttagggatg ctggccgagt tcgaagggtg cttgcggaag ggaggcgctt ggtggtaccc
      541 acgtctattc tgggagtcgg gattgctggg gcaggtctta tacttggaag cggaagcggc
      601 gggggtgcgg gccactggga tcggatgctt cttcgacgat ccggtgcatg agatcgtggg
      661 gatcaaggag tattcgcttc agtcgctcta tcactttacg gtaggtgggc cggttgatga
      721 cacaagattg atgacattac ccgcctacca ccatctcgtt tgatgagatc atctagttat
      781 tgtttatcaa tatcttggtc gtacattcat catgtttaaa ataaagaaaa aatccggtaa
      841 gccgaaggcc acgattcttt acaatgtggt tgaagactat tccgagttcg acgcaccggg
      901 taatgtcacg gtgtgcgcta tgacttacaa agaggacctg attgcacatg ccaaggttct
      961 atccgagagc tatgacatcc cgctggagac gatgactagg cttctcaccg aaggcggggt
     1021 ctatgtgtat ccccaggtcg gcggggtact gacgcgggga ctatttgcct gtcgaatcga
     1081 tccaactgga gagattccga aacagacctg gaccttggat ctcatgcagc ttgccgaggc
     1141 ggagcaattg gctcaagccc gggggattcc gcttgaagaa gttgcggcac tggtctttca
     1201 ctggacgttg ccggaagagt tacggaccaa gctcgagcag aagaatcttg ggatcgatta
     1261 ccgcacgttg gatcgcgccg tcgccaaggg cggcgatatc aagtacatcg atttccggaa
     1321 ggattggtcc ccgcatttca agcgcctttg catcatgccg gatggtcgac ttgttgagac
     1381 gggaggattg gaggagtttg cacagctcca tgggattacg tcggctcagg ccaggacgtt
     1441 ggtcgagcag ggaggtacgc tcgaagtgaa cggcgagctc ctggcttgtc agattgtgaa
     1501 cggtcagccg gcagtggcgc ggttcagcgc aaagaattac agtaaggcaa aagaattggc
     1561 taagaccaag ggcctgcacg tgatggatgc gctcagcgaa gtaggctaca acgatccggc
     1621 aatgatgcgc gggcttgtgc ggaaagagct cagtgttcga tcctgagtag aattcagaat
     1681 attgtgaagt aaagggggtg cgttacttgg cgagttcttg gttgatggct tcgacgagtt
     1741 tgtcgaggtt gaaaggtttt tcgaagacac ggcgagcgcc aaagagtttt gcgacgtcga
     1801 ggaaattgcg ttcgccttgg gcaccggtca tggcaatgac tttcgcatcg agatattccc
     1861 gcgtcaattg taaggtggct tcgagaccgt cggttccagg catcagaata tccatgagga
     1921 ccaagtctac tttgttctgc tggtagagcc tcaaaccctc tcggccatcg ctggcttcga
     1981 gcacatggtg tttggccctc tcaaggactt ccttcaggag gttccggatc gattcttcgt
     2041 cgtcgataac aagaatagtg gccatcgtat gagcttcacc cctaattttt tgatcttacc
     2101 tatgttgggt tctcctgtct agggagaagt tgaaagaagg ggaatcttac atgttgatcg
     2161 cgaaggtgcg cagcgcctgc tgctcgccag cggcggagat gaagagaaaa agattcgaag
     2221 agggggcgcg gtagggttga ctggctctag gacatttgat acaagagcgc ccattgtctg
     2281 acagactgca gtgcacttct ctcggagtgg ttctttgcag gatcctgatt cgtggtcttg
     2341 gactcgtcga tccctcttaa agacgtctgt tgtcgggctt cttttgctcg gtagcagatt
     2401 gctcagtcct tctcttgcgc gtgcgaacga acttcctgat ggggaactca ccttcttcaa
     2461 cgtgcgcacg aacgaacgac tgcgagttcg gtatcgtgat gacgcaggca actacgacct
     2521 gatggcgctg gatgaggtga atcatattct gcgttgccat cacacaggag aagtggccgc
     2581 catcgatgtg cgcatgctgg agcatgtgaa tcttgtgcag aacacagtgg gtggcgggaa
     2641 cgagatccac gtgatttcag gctatcggtc gcccgagtac aacgcacagc ttgtgaaacg
     2701 aagccggcga gtcgcgcaac acagcctaca tgtccaggga caggcgctcg acttctatat
     2761 tcccggtgtc aaggttcgtg agcttcgcca tgctgcattg aagttgcact atggtggggt
     2821 gggcgtctac cctcgcgcga aattcatcca tctcgattgc ggccccttca ggacctggta
     2881 gttctccccc tagcaagcga caggaaaact attgtagtcc gcgccctggg acttcgatgg
     2941 tcctcccgtg gggcacaaca acgaacatcc caggcagaac gctcaaaaag tccgtccagc
     3001 aaggccgcag cgagtgaagg cccgaggc
//
LOCUS       DATIPF010000174         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_474805, whole genome shotgun sequence.
ACCESSION   DATIPF010000174 DATIPF010000000
VERSION     DATIPF010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474805"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1968
                     /gene="gcvP"
                     /locus_tag="VLL06_04525"
     CDS             <1..1968
                     /gene="gcvP"
                     /locus_tag="VLL06_04525"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HSS30263.1"
                     /translation="SKDVTGKTAYRLSLQTREQHIRREKATSNICTAQVLLAIMASMY
                     AVYHGPEGLRRIAERVHGLAVVLAEGLRQLGFDVAAETYFDTIRVRLTKMQSEPIVFR
                     ANEAGINLRRHEDHSIGIALDEVSTEDEIQRLMEIFVGHDRLPFHVRTLSQNVTTSFP
                     ANLARTSAYLTHEVFNRSHAEHEMLRYIHRLETKDLSLVHSMIPLGSCTMKLNATAEM
                     LPVTWPEFSRLHPFAPVEQTKGYQELFHQLESWLAEITGLSAVSLQPNAGSQGEYTGL
                     MLIRAYHRSQGEEHRDVCLIPLSAHGTNPASAAMVGMTVVTVACDRQGNVDVTDLEVK
                     AAQHRDRLSALMLTYPSTHGVFEADVRRICQIIHTHGGQVYMDGANMNAQVGLCRPGD
                     IGADVCHLNLHKTFCIPHGGGGPGMGPICVARHLAPFLPGHPVTQLGGQDSIGSVSAA
                     PYGSPSIVTISWVYIALMGRDGMTKATQVAILSANYMAKRLEKYYPVLYTGPSQLVAH
                     EFILDLRQFKEAAGIEAMDVAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKAELDRF
                     CEAMIFIHAEIQDIAEGRQPRTNNLLKNAPHTAQVVAANEWTRPYSREQAAFPAPWVR
                     DHKFWPSVSRIDEAYGDRHLVCTCPPTDTYS"
BASE COUNT          453 a          598 c          605 g          441 t
ORIGIN      
        1 tccaaagacg tgacggggaa gacggcttac cgcctttccc tacagactcg tgaacaacac
       61 atccgacgtg agaaggcaac gagcaacatt tgtacggcgc aggtgctctt agccatcatg
      121 gcgagcatgt atgcggtcta ccacggaccg gagggcttgc gacggattgc cgagcgcgta
      181 cacggcctgg ccgtggtgct ggccgaggga ttgcggcagc tcggattcga tgtcgcagca
      241 gaaacgtact tcgatacgat tcgagttcgc ctgacgaaga tgcaatccga acccatcgtg
      301 tttcgggcaa acgaagccgg catcaatctt cgccggcatg aggaccactc catcggcatt
      361 gcgctggatg aggtgagcac ggaggacgaa atccaacgcc tgatggaaat ctttgtcggc
      421 catgatcgcc taccttttca cgtgaggacc ttgagccaaa atgtcacgac gagtttccct
      481 gcaaatctcg cccgtaccag cgcatacctg acccacgaag tgttcaatcg ctcgcatgcg
      541 gagcatgaga tgctgcggta catccaccgt cttgagacga aggatctgtc cctcgtccat
      601 tcgatgatcc cgttggggtc ctgcaccatg aaactcaacg cgacggccga gatgctgccg
      661 gtcacctggc ccgagttttc acggcttcat ccttttgccc cggttgagca aacgaaaggg
      721 tatcaagaat tgttccatca gctggagagt tggctagctg agattaccgg actttccgcc
      781 gtgtccttgc agccgaacgc aggttctcag ggcgaatata cgggactgat gttaatccgg
      841 gcgtaccacc gaagccaggg tgaagagcat cgggatgtgt gcttgattcc cttgtccgcg
      901 cacggcacga atccggccag tgcggcgatg gtgggtatga cggttgtgac ggtcgcctgc
      961 gatcgacagg gcaacgtgga tgtcaccgat cttgaagtca aggccgctca gcatcgtgat
     1021 cggctgtccg cgctcatgtt gacctacccc tcgacgcacg gtgtcttcga ggcagacgta
     1081 cggcgcatct gtcagatcat tcatacgcac ggcgggcaag tctatatgga cggtgccaac
     1141 atgaacgcac aggtagggct ctgtcgacca ggggacatcg gcgcggacgt ctgccatctg
     1201 aatctccaca aaacattctg tatccctcat ggcggggggg gacctgggat gggaccaatc
     1261 tgtgtcgctc ggcatctggc cccatttctt cccggccatc cggtcacaca attgggcgga
     1321 caagattcca tcggatcggt gtcggcggca ccctatggta gccccagtat tgtgacgatt
     1381 tcctgggtct acatcgccct catgggtcgc gatggcatga ccaaagccac gcaggtcgca
     1441 atcttaagcg ccaattacat ggccaaacgg ctggagaagt actatcccgt tctctataca
     1501 ggaccgtccc aactcgttgc gcacgagttc attctcgatc ttcgtcagtt caaagaggct
     1561 gcgggaattg aagcgatgga tgtggccaag cgattgatgg attatgggtt tcacgctccg
     1621 acggtctcgt tcccggtcgc cgggactctc atgatcgaac cgacagagag cgaatcgaag
     1681 gctgagttag accggttctg cgaagcgatg atctttatcc acgcggagat acaagacatt
     1741 gccgagggac gccagccccg caccaataat ctcttgaaga atgctcccca cacggcacag
     1801 gtggtggcgg ccaatgaatg gactcgaccc tatagccgtg agcaggctgc cttccctgct
     1861 ccctgggtac gtgaccataa attctggccc agcgtgagcc gtatcgacga agcctatgga
     1921 gatcgccatc tggtgtgcac gtgcccgcca acagacacct actcataggg ctgctgaaaa
     1981 aggtctctga cttcgttctc aatcgcgtgc ctccctgcga cgtaccgcgc acggggaaag
     2041 agctgttctg acagctcggg gttgggcggg tgagaaagtt acgcctcagt cgtcccg
//
LOCUS       DATIPF010000175         6027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_830279, whole genome shotgun sequence.
ACCESSION   DATIPF010000175 DATIPF010000000
VERSION     DATIPF010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830279"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..184)
                     /locus_tag="VLL06_04530"
     CDS             complement(2..184)
                     /locus_tag="VLL06_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30264.1"
                     /translation="MRILVFCDEDLGVSAGGARQVLEFAQALSQRSHTIRIVAPQPAQ
                     GRQLAEEFASLSFRAV"
     gene            complement(162..1349)
                     /locus_tag="VLL06_04535"
     CDS             complement(162..1349)
                     /locus_tag="VLL06_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSS30265.1"
                     /translation="MRWFLFCYWYEPDAPSDPVGLVRLWTLARQLVERGDSVTVLPPR
                     YRSALLQRGFSTSPIPLLPWPVLRPMSYAVLSSLIGLMRAWRSKPEVVYYRWMDSPHP
                     LVFARLVGAQCVCEVNGEPVPDWSETDRRLKRRLKHWLASWALSRCDRVVVLTDGLRD
                     LVVRRYRVAADRVAVLPSGTDTDLFVPQDLAACRRRIGVGLERDYIGFVGSFYRYQGL
                     QCLLDSMAIITRTMPSVHLLMVGDGEAFVELKQQADRLGLNPCITWPGRIPYQEVPTW
                     IGGMTVCVAPFRGDRGETSPVKIYDYLACGKPVIASAIPSVSATFVQGTGVAFVPPDD
                     PSALARELLALLNDPDRQARMATAGRRYVEQRFSWRHITDRLREWVVTEKASIHHAHS
                     RVL"
     gene            complement(1346..2716)
                     /locus_tag="VLL06_04540"
     CDS             complement(1346..2716)
                     /locus_tag="VLL06_04540"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HSS30266.1"
                     /translation="MVKPQDAVIVSVLAAVLALGLTMTTPVVGIQVVFGFLIVMVAFT
                     SVPASLFLLIGSMLLSPEIAIGRVDGRGVGVRDLSLRVDDLLLVIIGFSWLVKTIIYR
                     DLALIRETPINRPIVYYMLVCVLATLIGVIAGRVRPLTGFFFILKYFEYFFVFFMVVN
                     HVSTKRQIVSLVTALLAICFVISVYAIVQIPSGQRASAPFEGESGEPNTLGGYLVFIM
                     AIVMGLFLHLKSGPIRVLLGLLMGFISLALMATLSRSSYLAAGVLMLAVAATQWRRPG
                     VLALILLLGVMLPFAAPDNVKNRVKETFFGRQYGGEIKVGRVGLDLSTTERLRSWQYV
                     LKDWLHNPVLGRGVTGYAWADAQYVKILGETGLAGLVAFIFLIFRLWRRARETFLAEQ
                     DPFCKGLAHGLLLGMVAMLAHGIGANTFIIIRIMEPFWLCAGLVMILPRLTSEQKDPA
                     PQRALV"
     gene            complement(2753..4891)
                     /locus_tag="VLL06_04545"
     CDS             complement(2753..4891)
                     /locus_tag="VLL06_04545"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HSS30267.1"
                     /translation="MAQYELNVIDYWLIIKKRRYLILLAASLVVSFTFLFSEMFKPSP
                     LYEASALVKFDRASTVAQQLIETMSFSNANDLNSQTEFIRGFPVMERVAVELGRVSAE
                     VSPEDKRSATYLNTVYNLGQEIRTQREGDTNIIRITAITDQPEMAEKTANSVANAYRL
                     ENIMTRNRLVMESRRFVEEQLAGLEKQLNDAEDALRAFREREGQVFLTDEARAALETF
                     TKLEEQVNDVARKRAEGERQISVLNRTEAVLGNQTGRIFTEELNALLTILNQRLLDLL
                     QERNTLLINYTTDHPQVLEQQKKIDNVRAEMVLELRAKLKTMQDREATLLDQRDRYRN
                     RYLQFPRAAIEMSRLEREVKVNGDLLATLKSKHQELQIKSAERIEEVTIIAPAIMPGG
                     PINSPNTGLNLMVGSLMGVFLGIVLAFARESFDTSIGTIEGVEEFLKVPVLGVIPQFD
                     SKEMEEAARAALPAHASASAVENFSKLICLIDPKSPLSESLRSLRTNIQFASMDRKVK
                     SILFTSAGLGEGKSTCVTNLAITLAQEGQRVLLVDADLRRPIVHQRLGLERVPGLSDA
                     LVGSTLWRSHVRSATDLMLGPMGADRVMSTPGLDNLHILTSGSEVGNPNEFLNMAKIK
                     TFVNEMNEEYDLVLFDSPPILPVTDAVAFSSRVDGTILVYQVGRIGRNALKRAKFLLD
                     HAQANVMGIVLTNVKSEVASDSGVYRYEYR"
     gene            complement(4941..5732)
                     /locus_tag="VLL06_04550"
     CDS             complement(4941..5732)
                     /locus_tag="VLL06_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HSS30268.1"
                     /translation="MRQLIPRYLIGLLGAGAILAGGFLGCHNPASSSLPPSAISPPGT
                     AVPPLPKGDVYADETVPTSETPIETGDTLEVVIRRGAGEEKFTNAVRENGKISVGFME
                     VDVGGVTAAVAEARVQEAAVPYMRQPSVQVLLKKKVLKVKRVFVFGDVKKPGMVPMQR
                     HMTVLQAIAAADMYQETALMEEIRVIRGGDLAKPHILMADLARAFTYGDMTRNISLEE
                     NDIVFVPREHMGDASEAGKIIMPIITSGIAPFYPVFLLNAITTTK"
     gene            complement(5762..>6027)
                     /locus_tag="VLL06_04555"
     CDS             complement(5762..>6027)
                     /locus_tag="VLL06_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30269.1"
                     /translation="LLANGKTVFPYHLLKALVDQLSIYPLGTTVRLNTGETGVVSQLN
                     RQYPLRPIVQVSQQAASAQASASKTVDLRAETSLHIVEVVPIG"
BASE COUNT         1398 a         1766 c         1620 g         1243 t
ORIGIN      
        1 tctagacggc acggaacgac agcgaggcaa actcctccgc cagctgtcgt ccttgtgcag
       61 gctggggcgc cacgatccgg atggtatggg aacgttggga aagggcttgg gcaaactcca
      121 acacctgccg cgctccgcca gccgaaacac ccaagtcttc atcacagaac acgagaatgc
      181 gcatggtgta tcgatgcctt ttcggtaaca acccattctc tcaagcgatc tgtaatgtgt
      241 ctccaactaa accgttgttc aacatagcgg cgcccagcgg ttgccattcg agcctgacgg
      301 tccggatcgt ttaacagcgc cagcagctcc cgagcgagtg cggacggatc gtccggggga
      361 acgaacgcga ctccggtccc ttgaacaaac gttgcggaga cggaggggat cgcactggcg
      421 atcacgggct ttccgcaggc aaggtaatca tagattttga ccggagacgt ttcgccacgg
      481 tcccctcgga acggagcgac gcagactgtc atgccaccga tccacgtcgg aacctcctga
      541 tacggaatcc tgccgggcca ggtaatacag ggattcagtc ccagacgatc cgcctgttgt
      601 ttcaattcca caaaagcttc tccgtcgcca accatgagta gatgcacaga tggcatcgtt
      661 cgtgtgatga tggccatgga atccagcaaa cattgcaacc cttgatagcg gtagaaactc
      721 ccgacaaaac cgatgtagtc tcgctccagg cccactccga ttcgacgccg gcaggccgcg
      781 agatcctgag ggacaaacaa gtctgtatcc gttccactgg gcagcacggc cacgcgatca
      841 gccgcaacac gataacggcg gacaacgaga tctcgtaatc catcagtcag aacaaccacg
      901 cgatcgcagc ggctgagggc ccacgatgcc aaccagtgtt tgagtcttcg tttaagcctg
      961 cgatcggtct ctgaccaatc gggcaccggc tccccgttca cttcacagac gcactgcgct
     1021 ccgaccaatc ttgcaaatac caacggatgc ggactgtcca tccaccggta atagacgact
     1081 tccggtttcg accgccaggc tcgcatgagc ccgataagac tggagagcac cgcataggac
     1141 atcggacgca gcaccggcca aggaaggagc ggaatcggcg aagtcgaaaa gccccgctgg
     1201 agcaacgcgg atcgataccg aggagggagg actgtgacac tgtcgccccg ttcgacaagc
     1261 tgacgcgcca aggtccagag acgaacgagt cctacgggat cggatggcgc atccggttca
     1321 taccaataac aaaagaggaa ccacctcata cgagtgctcg ctgaggggca ggatccttct
     1381 gctccgatgt cagacgcggc aagatcatga caagcccggc acacaaccaa aagggctcca
     1441 tgatgcgaat gataatgaac gtgttggctc cgataccatg ggcgagcatg gcgaccatgc
     1501 caagcagcaa tccatgggcc aaccccttac agaagggatc ctgttcggcc agaaaggtct
     1561 ctcgtgcgcg ccgccacaag cggaagatca agaagataaa cgccacgagg ccggccagac
     1621 ccgtttcgcc gaggattttg acgtattggg cgtccgccca ggcatagccc gtcaccccgc
     1681 ggccgagcac agggttgtga agccaatcct tcagcacata ctgccaggaa cgcaaccgct
     1741 ctgttgtgga gaggtccaac cctacgcgtc ccactttgat ttcgccgcca tattgacgtc
     1801 cgaagaatgt ctccttgacc cgattcttga cattgtcagg cgccgcaaag ggtagcatca
     1861 ccccgagcag tagtatgagc gcgagcacac cgggtcgccg ccattgcgtc gcggcgacag
     1921 ccagcatcaa caccccggca gccagatagg atgaacggga gagcgtggcc atgagagcca
     1981 gactgatgaa tcccatgaga agtccgagca gcacccgtat cggtccggac ttcaagtgca
     2041 gaaagaggcc catgacgatg gccatgataa acaccaggta tccgccgagc gtattgggtt
     2101 cgccactctc tccttcaaac ggggcgcttg cccgttgacc gcttggaatc tgaacgatcg
     2161 catagacact gatcacgaag cagatcgcca gcaatgccgt caccagactg acaatctgcc
     2221 gtttggtcga cacatgattg acgaccatga aaaacacaaa gaagtattcg aaatatttca
     2281 aaataaaaaa gaatcctgtg agcggacgga cacgcccggc gatgacgccg atcagcgtcg
     2341 cgagcacaca gaccaacatg taatagacga tggggcgatt gatcggggtc tcgcgaatga
     2401 gggcgagatc gcgatagatg atcgtcttga cgagccaact gaacccgatg atcacgagca
     2461 agagatcgtc cacgcgcaac gagaggtccc gcacacccac gcctctccca tccactcgtc
     2521 caatcgcgat ttccggggag agcaacatgg aaccgatgag gaggaagagc gaggcgggaa
     2581 ccgacgtgaa cgccaccatg acaatgagaa acccgaacac cacttgtatg ccgacaaccg
     2641 gggtcgtcat cgtaagacca agtgccagca cagcggccag caccgagacg atgacggcat
     2701 cttgcggttt caccatcgac gcatgcatag ggtgtcggct cctactggcg cgtcagcgat
     2761 attcgtatcg gtagacaccg gaatctgatg cgacctcgga cttcacgttc gtcaagacaa
     2821 tgcccatgac attggcctgc gcatggtcta ggaggaactt agcccgtttg agggcattcc
     2881 gaccgatccg gccgacttga tacaccagga tcgtcccgtc gacgcgtgag ctgaacgcga
     2941 ccgcatccgt gaccgggaga ataggaggag aatcaaagag cacgaggtcg tattcctcat
     3001 tcatttcatt gacgaatgtc ttgatcttcg ccatgttgag gaactcgttc gggttgccta
     3061 cctccgaccc gctggtcaga atatgcaggt tgtccaaccc cggcgtgctc atcacccgat
     3121 ccgctcccat cggcccgagc atcagatccg tggcggaccg cacgtggctg cgccagagag
     3181 tactgcccac cagagcatct gacaggcccg gaacccgttc gagacccaac cgctgatgca
     3241 caatcggcct tcgtaggtca gcatcaacga gtaacactcg ttgtccctcc tgagcgaggg
     3301 taatcgccag attggtcaca caggtgctct tgccttcgcc aagacctgcg ctggtaaaga
     3361 ggatggattt gaccttccga tccatgctgg cgaattgaat attcgtccgc agggaccgca
     3421 gactctcgga caggggagac ttcggatcga tcaggcaaat gagtttcgag aagttttcca
     3481 cagccgacgc tgaggcgtgg gctggaagcg ctgcgcgggc ggcttcttcc atttctttac
     3541 tgtcgaactg cgggatgacg ccaagaaccg gcactttgag aaattcctcg actccctcga
     3601 tggtaccgat cgaggtgtcg aatgattcgc gcgcaaaggc caagacaatc ccaagaaaga
     3661 cgcccatgag agagccaacc atgagattga gccccgtatt gggggaattg atcggtccac
     3721 caggcatgat ggcaggtgca ataatcgtca cctcttcaat ccgctcggca ctcttgatct
     3781 gtagttcttg atgcttgctc ttgagtgtcg ccaggagatc gccgttcact tttacttcac
     3841 gctcaaggcg actcatttca atcgccgcgc gtgggaactg taaatagcga tttctatagc
     3901 gatcccgctg atccagaagc gttgcttccc ggtcttgcat ggtcttgagc ttggctcgga
     3961 gttcaagaac catctcggct ctgacattat cgatcttctt ctgctgctcc agaacttgcg
     4021 ggtgatctgt tgtgtagttg atcagaagag tgttccgttc ctggaggaga tcgagaaggc
     4081 gctgattgag aatcgtgagc aacgcattca gttcttctgt gaagatccga ccggtttggt
     4141 tccccagcac cgcttcagtt cgattgagca cggagatctg ccgttccccc tccgcccgct
     4201 ttcgcgcgac gtcgttgacc tgctcttcca gcttggtaaa tgtctccagc gcggcgcgcg
     4261 cctcatcggt caagaagacc tgtccttccc gttccctaaa ggcccgtagc gcgtcttccg
     4321 catcgttcaa ctgtttttca agcccggcca gctgttcctc gacaaaccgt cgtgactcca
     4381 tgaccagacg attccgggtc atgatatttt ccagacgata ggcattggcc actgaattgg
     4441 ccgtcttctc tgccatctcc ggttgatccg tgatagccgt gatacgaata atgttcgtat
     4501 ccccctcccg ctgcgttctg atttcctgac cgaggttata cacggtgttc agataggtgg
     4561 ccgatcgctt gtcttccggc gacacctctg ccgatacgcg acctaattcc accgccacgc
     4621 gctccatcac agggaacccc cggataaact cagtttggga gttgagatcg ttggcgttgg
     4681 aaaaggacat ggtctcgatc aactgctggg ccaccgtgct cgctcggtcg aacttcacga
     4741 gggcagacgc ttcatacagc gggctcggct tgaacatttc tgaaaagagg aatgtgaatg
     4801 acaccacgag gctagctgcc agcagaatca gatacctccg cttcttgatg atcagccagt
     4861 agtcgatgac attgagttcg tattgggcca taggatctca gtgtccttgt cttgtctcga
     4921 tcgtgacgat catcgctctg tcacttcgtt gttgttatcg cgttgagaag aaatactgga
     4981 tagaagggtg caattccaga cgtaatgatc ggcattatga ttttcccggc ttcggaggca
     5041 tcacccatat gttcgcgcgg cacgaacaca atgtcgtttt cctccagcga aatgttcctc
     5101 gtcatatcgc cataggtaaa cgcgcgcgcg agatcggcca tgaggatgtg tggctttgcg
     5161 aggtctccgc ctcgaatgac ccggatttcc tccatcaatg ccgtttcctg atacatgtct
     5221 gcagcggcaa tggcttgcag gaccgtcata tgtctctgca tgggaaccat cccaggtttc
     5281 ttcacatccc caaacacgaa tacgcgcttg accttcagca ctttcttttt gagcaacacc
     5341 tgcacacttg gctgacgcat gtagggtacc gcggcttcct ggacacgggc ttccgctacc
     5401 gccgcagtga cacccccgac atccacctcc ataaagccga cagaaatctt tccattctca
     5461 cgcacagcat tggtgaattt ctcctcgccg gcaccgcgcc ggatcaccac ctcaagcgtg
     5521 tcaccggttt cgataggtgt ttccgaggtc gggacggttt catcggcata gacatctccc
     5581 ttgggcaacg gcgggaccgc cgtgccagga ggactgattg ccgacggcgg caaggacgag
     5641 cttgccgggt tgtggcaccc aagaaaccct ccagccaaaa tcgcgccagc acccagaaga
     5701 ccgatgagat atcgaggtat gagctgtcgc acggccttat ctcctatgct cctattgcag
     5761 atcaaccgat gggcaccact tccacaatgt gcagagaggt ctcggctcgc aaatccaccg
     5821 tcttcgatgc cgatgcctgt gctgatgcag cctgttgcga gacttgcacg attggacgca
     5881 gcgggtactg ccgattcaac tgagatacga ccccggtttc tccggtgttc agacgaacag
     5941 tcgtgcctaa cggatagatc gaaagttggt cgaccaacgc ctttaagaga tgatagggaa
     6001 acaccgtttt gccgtttgcg agaagcg
//
LOCUS       DATIPF010000176         3402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1537140, whole genome shotgun sequence.
ACCESSION   DATIPF010000176 DATIPF010000000
VERSION     DATIPF010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537140"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VLL06_04560"
     CDS             complement(<1..528)
                     /locus_tag="VLL06_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HSS30270.1"
                     /translation="MERDTKTYVLKRPDEEALPRKLSIDYRAALNSQQLAAVTAGEGP
                     SLVIAGAGSGKTRTLVYRVAYLIDSGIDPSNILLLTFTRKSAQEMLARVGDLIGARSQ
                     RVCGGTFHSVANLLLRRYGRSIGVEPGFTILDRGDAEDLIALMRAQLGLNEKDKRFPR
                     KGTIMEMLSKCENTLR"
     gene            830..1690
                     /locus_tag="VLL06_04565"
     CDS             830..1690
                     /locus_tag="VLL06_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30271.1"
                     /translation="MAKTEYQQQLRALAELMLAVSGESVTVMKKNAPEQALKLKRQDE
                     WGLYMEFLKVMFNLTDRLSALYLPIKEQPQFMDNLEDAVTVQLKQVLEPAFGSGADHM
                     EIMLTIGAAVAESRQIYERYRFLVTEDNPAKDEMMNDFGGRVADAMGTAGNTLIISAA
                     TLCARAAIPAITAILAGDTPSVAAIGTAAPLVATDPNSAPSLPTSAPKPQGPTGTEIK
                     LVSVMSTVTGEEVETRWGLHPRFRQDLSPEDIQHIAKLMNRVTKILGERYASVAFSDE
                     WTSWHKAGHA"
     gene            1844..>3402
                     /gene="glgP"
                     /locus_tag="VLL06_04570"
     CDS             1844..>3402
                     /gene="glgP"
                     /locus_tag="VLL06_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HSS30272.1"
                     /translation="MDTPQSADKSSQAIPTGLSRLKELAMNLWWSWTPEARRLFEHID
                     PTLWVLTNHNPVKLLAEVRPDRLIHLAQDPSYMRRYSAALKMFDEYRQNEHSWFTTQH
                     SDLHAPTIAYFSAEFGLHTSIPIYSGGLGILAGDHCKEASDLGVPLVGVGFMYPQGYF
                     RQRITPEGWQEAAYAPFNRAVSPVHPALTPSGDACRITVEIGSRVVAAAVWKVLVGLV
                     PLYLIDTDVPENSPENRALSARLYGGDQEMRLCQEYLLGIGGVRMLRALGIAPTVWHA
                     NEGHSAFLTLERIRELVQKGSTHADACEIIRQSTVFTTHTPVPAGHDVFPHDLMDRYF
                     TGYWEQVGLSREDFLRLGDTPESQRQGFNMTALAIRMAAHVNGVSREHGQVSREMWRH
                     MWPGLPIEQVPIRSVTNGIHAPTWIAPELNQLYGKYLGPEWAEKCDDTAMWQRVLDIP
                     DQELWAVLQAMKRKLMSFIRERARSGWMQGHLQASQVLTRGTLLDPEALTIGFARRFA
                     TYKRATLLFRDL"
BASE COUNT          778 a         1004 c          924 g          696 t
ORIGIN      
        1 gcgaagcgta ttttcgcact tgctcagcat ctccatgatc gtccccttgc ggggaaaacg
       61 cttgtccttt tcgttcagcc ccaactgggc ccgcatcagc gcaatgagat cttccgcatc
      121 gcctcgatcc agaatcgtga agcccggctc gactcctatc gaccggccat agcgccgcaa
      181 caagaggttc gccacagaat ggaacgtgcc gccacacact cgttgactcc tagcaccgat
      241 caagtcgccc acacgagcca acatttcttg agccgacttg cgcgtaaacg tgagcagcag
      301 aatgttcgag ggatcgatgc cggaatcgat cagatacgcc acgcgataaa ccagcgtacg
      361 ggtcttgccg ctgccggcgc cggcaatcac cagtgagggg ccttcccccg ccgtcaccgc
      421 cgccagctgt tgggagttca atgctgcgcg atagtcgatg gagagtttgc gcggtaacgc
      481 ctcctcgtca ggccgtttca gcacatatgt cttcgtgtct cgttccatag aagttccaca
      541 agaagcccag acgacatcat aacaagcggg ctgcgctgta taacattacg gacaagggcc
      601 tttcaacgag ggagcacagg ctcagaaaaa tccttcactg ggcgtgcgat tcaacagcat
      661 cgagattcac ggaggggaac caaaatgagg ccgccgatac tgtaaataac ggcgcccggt
      721 ctttacaatg tgattgcggt ttttgaataa ccccgcctgc ccattggagc ctgcaccaca
      781 acctgtatat aatgatctcc tcatttctct tggagtaccg aggaacacca tggccaaaac
      841 cgaataccag caacaactcc gcgccctggc tgagttgatg ctcgccgtat cgggtgaatc
      901 ggtcaccgtg atgaagaaga acgcccctga acaggcgctg aaactcaaac gtcaggatga
      961 atggggcctc tatatggagt ttctcaaagt gatgttcaat ctgaccgacc ggctctccgc
     1021 actgtacttg ccgattaaag aacagcctca attcatggac aatctcgagg atgccgtcac
     1081 ggtgcaattg aaacaggtat tggaaccggc attcggttcc ggggccgatc acatggaaat
     1141 catgctcacg ataggtgcgg cagtcgcaga gagtcggcag atctatgagc gttatcggtt
     1201 tctcgtcacc gaagacaatc ccgcaaagga cgagatgatg aacgactttg ggggacgagt
     1261 cgccgatgcg atgggcactg cgggaaatac gctgatcatc tccgctgcaa cgctctgtgc
     1321 acgtgccgcg attccggcca tcaccgcaat cttggctgga gacaccccct cagtcgccgc
     1381 gatcggcaca gccgcgcccc tcgtggcgac cgatccaaac tccgcaccat cactccctac
     1441 ttcggcacct aaaccccagg gaccgacggg aactgagatc aagttggtga gtgtgatgtc
     1501 gacggtcacc ggagaagaag tggaaactcg atgggggctg caccctcggt ttcgacaaga
     1561 cctttcgcca gaggacatac agcacatcgc caaactgatg aatcgagtga ctaaaatact
     1621 tggcgaacgc tatgcgtccg tggcattctc agacgaatgg acgtcttggc ataaggccgg
     1681 tcatgcctga ccgattctga caatccagtc tctttggctt gtttggtcta tctgattttt
     1741 ctggtgtatc catttagtct ggttcaacca cacacaagac agaccacatc aaccaaatag
     1801 tccagataaa ccagataaac ccccagaatt ttgaggaggt gcagtggata ctccgcaatc
     1861 agcagataag tcatcacagg ccattccaac cggactctca cgtctcaagg aattggcgat
     1921 gaatctttgg tggagttgga ctccggaggc ccgacggctg tttgagcaca tcgacccgac
     1981 cctctgggta ctgaccaatc acaatcccgt caaactcctg gcagaggtca gacccgaccg
     2041 actcatacac ctcgctcaag atccctcgta catgcggcgg tactctgccg ctctcaagat
     2101 gttcgacgag taccgtcaga acgagcacag ctggtttaca acacaacaca gcgatttgca
     2161 cgctcccacc atcgcctatt tctctgcgga attcggtcta catacgtcca ttcccattta
     2221 cagcggcggt ctgggcattc tcgccggaga ccattgcaag gaagccagcg atctcggtgt
     2281 gccgctggtg ggagtgggat tcatgtaccc acaaggatat ttccggcagc gaatcacgcc
     2341 ggagggatgg caggaggcgg cctacgcccc gttcaaccgg gcagtctcgc cggttcatcc
     2401 cgccctcact ccatcgggag atgcgtgcag gattacggtc gagatcggaa gccgggttgt
     2461 ggccgcagcg gtctggaagg tgctcgttgg gctggttccc ctctacctta tcgatacgga
     2521 cgtcccggag aacagtcctg agaatcgggc tctctcagcc cgactgtatg gcggggatca
     2581 ggaaatgcgt ctctgccagg agtatctgtt gggtatcggc ggtgtccgca tgcttcgggc
     2641 tttgggtatc gcaccgacag tctggcatgc caatgagggg cactccgcct tcctcacgtt
     2701 ggagcgtatt cgcgaattgg ttcagaaggg atcgacgcac gcggacgcct gcgagatcat
     2761 ccgccagagc acggtgttta ccacccatac accggtgccg gccggtcacg acgtatttcc
     2821 gcatgatctc atggaccggt actttaccgg ctattgggag caggtgggcc tctctcgaga
     2881 agactttctc cgcctgggag atacccccga atcccagagg cagggtttca acatgacggc
     2941 gctcgcgatc cggatggctg cccatgtgaa cggggtcagc cgtgaacacg gacaggtctc
     3001 gcgggagatg tggcgccaca tgtggcccgg cctcccgatc gaacaagtcc cgatccgcag
     3061 tgtgacgaac ggcatccatg caccgacctg gatcgcgccg gaactcaacc aactctacgg
     3121 caagtatctg ggccccgaat gggcggagaa atgcgatgat acggccatgt ggcaacgtgt
     3181 cctggatatc ccagaccaag aactctgggc cgtactccaa gccatgaaac gcaaactcat
     3241 gagcttcata cgggaacgtg ctcgaagcgg ctggatgcaa gggcatctcc aagcctcgca
     3301 ggtcttgacg cgaggcacgc tactggaccc cgaggcgctc actatcggct tcgccaggcg
     3361 ttttgccacc tacaagaggg cgaccttatt gttccgcgac ct
//
LOCUS       DATIPF010000177         3514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1184223, whole genome shotgun sequence.
ACCESSION   DATIPF010000177 DATIPF010000000
VERSION     DATIPF010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184223"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2913
                     /gene="carB"
                     /locus_tag="VLL06_04575"
     CDS             <1..2913
                     /gene="carB"
                     /locus_tag="VLL06_04575"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HSS30273.1"
                     /translation="GASAEAIHKAEDREAFKHAMQRIGLRVPASGTAHSREGAIKILE
                     QIGFPAIVRPSFTMGGTGGNIAYNREEFEKIVNWGLAMSPVGQLLIEESLIGWKEYEL
                     EVMRDLKDNVVIVCPIENLDPMGVHTGDSITIAPAMTLSDKEYQRMRDAAVRIMREIG
                     VDTGGSNVQFGLNPQNGDMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     ITNDITGVTKASFEPTIDYVVVKIPRFAFQKFRGADPTLTTQMKSVGEVMAIGRTFKE
                     SLQKAIRSMELNMNGLSSREGLDRGIPDGIDRQHAIERVRHHLRTPIAERLWHVADAI
                     RLGLSNDDLFALTKIDPWFLEQIRDLMQFEALLVGQGGKKSAALEESLLWEAKELGFS
                     DDRIAQLVGVTSASVRKQRTKAGQGRRRVTYKRVDTCAAEFEAHTPYLYSTYGSECEA
                     RPTDRKKVIILGGGPNRIGQGIEFDYCCVHAAMALREEGIESIMVNCNPETVSTDYDT
                     SDRLYFEPLTEEDVLNIVEREQPLGVVLQFGGQTPLKLALPLSKAGVRILGTSPEAID
                     RAEDRERFRDLLNTLGLRQAESGMARSVDEALRIASQISYPVMVRPSYVLGGRSMQIV
                     YDESDLLEYMKSAVKASPKHPVLIDKYLSDAIEVDADAISDGTNVVVAGIMEHIEEAG
                     VHSGDSACSLPPYSLGLDVVEEIRRQMKALALELGVIGLMNAQFAVKDQTIYVLEVNP
                     RGSRTVPFVSKAIGVPLAKLAMKAMVGKSLPELGLIEAPTPSHLSVKESVFPFNKFPG
                     VDVLLGPEMKSTGEVMGIDQDFGWAFAKSQAGAGAPLPKGGAAFISVKESDRPAALEL
                     VRQLQRLGFKVQATSGTGAYLRNHGVQAETVNKVMEGRPHIVDHIKNGQVALVVNTVS
                     TASSHADSLSIRREALNKGLAYYTTIRGARAAVMGIEAILKKELTIRSLQEYHHHS"
     gene            2928..3404
                     /gene="greA"
                     /locus_tag="VLL06_04580"
     CDS             2928..3404
                     /gene="greA"
                     /locus_tag="VLL06_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HSS30274.1"
                     /translation="MPTPITRKGHEALQAELDRLRKVERAKNIEAIAEARAHGDLSEN
                     AEYDAAKERQGFIESRIVELQSKLADARIIETAGRISETIVFGATVLVIEQETQSKRE
                     YTLVGQDEADMKVNRISIQSPVGKALIGKRVGDFVEVKTPVKTVEYEVVEIRFDEL"
BASE COUNT          827 a          881 c         1068 g          738 t
ORIGIN      
        1 ggtgcgtccg ctgaggcgat tcataaggcc gaggatcgcg aagccttcaa acatgccatg
       61 cagcggattg ggttgcgagt gccggccagc ggcacggcac atagccgtga aggcgccatc
      121 aagattctcg aacaaatcgg atttccggcg atcgtccgtc catcgtttac catgggcggg
      181 accgggggaa acatcgcgta caaccgggaa gagtttgaga aaattgtcaa ttggggcctg
      241 gcgatgagtc cagtcggtca actcttgatc gaagaatccc tgatcgggtg gaaagaatat
      301 gaacttgagg tgatgcggga tctcaaagac aatgtcgtga ttgtgtgtcc gattgaaaat
      361 ctggacccga tgggagtgca cacgggtgat agcatcacga ttgcgccggc catgacgctc
      421 tcggacaagg aataccagcg tatgcgggat gccgcagtac ggatcatgcg tgagatcggt
      481 gtcgatacag gcggatccaa cgtccagttc ggcctgaatc ctcaaaacgg cgacatggtc
      541 gtcatcgaaa tgaacccgcg tgtatcgagg agctctgcgc ttgcctcgaa ggcgacggga
      601 tttcctatcg ccaaaattgc ggcgaagctt gccgtgggtt acacgttgga tgaaatcacc
      661 aacgacatta ccggtgtgac gaaagcctcc ttcgaaccca cgatcgatta tgtggtggtg
      721 aagattcctc gttttgcctt ccaaaagttt cgaggggccg atcccaccct gacgacgcag
      781 atgaaatcgg tgggcgaagt gatggcgatt ggacggacct ttaaagagtc gttgcagaag
      841 gcgattcgtt cgatggagtt gaacatgaac gggctctcct cgcgggaagg cttggatcgt
      901 ggtattcctg acggcattga tcggcaacac gcgatagagc gggtacgcca ccatctgcgg
      961 accccgattg ccgagcgact gtggcacgtg gcggatgcga tacgccttgg cttgtccaat
     1021 gacgacctgt ttgccctcac gaagatcgat ccctggttcc tggagcagat ccgggatctg
     1081 atgcaatttg aagcgctgct ggtcggtcag gggggaaaga aatcagctgc gcttgaggag
     1141 tcgcttctgt gggaggccaa ggaacttggc ttttcagatg atcgtatcgc gcagctcgtc
     1201 ggcgtgacct cagcctcggt gaggaagcag cggacaaagg ccggtcaggg gcgaagacgg
     1261 gtgacctaca agcgggtcga cacgtgcgcc gcagagtttg aagcccatac cccgtatctc
     1321 tattcgacgt atggaagcga atgtgaggct cgcccgaccg accggaagaa agtgatcatc
     1381 ctcggtgggg gaccgaatcg tatcggacag ggaatcgaat tcgattactg ttgcgttcac
     1441 gcggccatgg cgcttcgcga agaaggtatc gaatcgatca tggtgaattg taatcctgaa
     1501 acagtgagta cggactatga cacctcggac cgactgtatt ttgagccgtt gactgaagaa
     1561 gacgtcctga atattgtgga gcgtgaacag ccgttgggag ttgtgttgca gttcggcggg
     1621 cagacgcccc tcaagctcgc gctgcccttg tccaaggccg gagtcagaat tctgggcacg
     1681 agtcccgagg cgatcgatcg tgcggaggat cgagaacgat ttcgcgacct cctaaatacg
     1741 ctcgggcttc gccaagcgga gagtggtatg gcccgatccg tagacgaggc tctgcggatc
     1801 gcctcgcaga tcagctatcc ggtcatggtg cggccctcgt atgtattggg tggccggtcc
     1861 atgcagattg tctacgacga atccgacttg ctcgaataca tgaaatcggc ggtcaaggcc
     1921 tcgccgaaac atcccgtgct gatcgacaaa tacctgtctg atgcgattga agtcgatgcc
     1981 gacgcaatct cagatgggac gaatgtcgtg gtggccggaa tcatggaaca tattgaagaa
     2041 gccggggtac attcgggaga ctcggcctgt tccctgccgc cctattcgct cggtctggat
     2101 gtggtcgaag agattcgtcg tcaaatgaag gccttggctc tggaactcgg cgtgatcggt
     2161 ctgatgaatg cacaatttgc tgtcaaagac caaacgatct acgtgctgga ggtcaatccc
     2221 cgtggctcgc gtacggtgcc gtttgtcagt aaagccatcg gcgtgccgct tgccaagctg
     2281 gccatgaagg ccatggtggg caagagcctt ccggaactgg gcctgatcga ggccccgaca
     2341 ccttcacatt tgtccgtcaa agaatccgta tttcccttca ataagttccc gggggtggat
     2401 gtcctcttgg gtcctgaaat gaagtccacc ggggaagtaa tgggcatcga ccaggatttc
     2461 ggatgggcct tcgcgaagtc ccaagccgga gctggtgcgc cgctgccgaa aggaggcgcg
     2521 gcattcatca gtgtgaagga aagtgatcgt ccggcggctc ttgaacttgt gcgtcagctc
     2581 caacgcctag gattcaaggt tcaagcgaca agtgggacgg gggcgtatct gcggaatcat
     2641 ggggttcaag cggaaaccgt gaataaggtc atggaagggc gaccccatat tgtggaccat
     2701 atcaaaaacg ggcaggtggc cctggttgtg aacacggtga gtaccgcatc gtcgcatgcc
     2761 gattcattgt caatccgtcg ggaagctctg aacaaaggtc tggcctacta cacgacaata
     2821 cggggggcac gagcggcggt tatgggcatt gaagccattc tcaagaaaga actcaccatc
     2881 cgatcgctgc aggaatatca ccaccactca tagaggacga cgagatcatg ccgacaccga
     2941 ttaccagaaa ggggcacgag gcgcttcaag cagagctcga tcgccttcgc aaagtcgagc
     3001 gcgccaagaa tatcgaagcc attgccgaag cgcgcgccca tggcgatttg agcgagaatg
     3061 cggagtacga tgccgccaag gaacgccagg gattcatcga gtcacgtatt gtggagctac
     3121 aatcgaaatt ggccgacgcc cgtatcatcg aaactgccgg acgaatctcc gagaccattg
     3181 tattcggtgc cacggtactt gtgattgaac aggagacgca atcgaaacgg gaatacacgt
     3241 tggtaggaca ggatgaagcc gatatgaaag tgaaccgtat ttcgatccag tctccggtcg
     3301 gtaaggcatt gatcggaaaa cgagtcggcg atttcgtcga ggtcaagaca ccggttaaga
     3361 cggtcgaata tgaggtggtg gagattcgct tcgatgagct gtaatccgca ttattcatga
     3421 gcctttctgt tgccatcgag gagacaaaaa tcacaaccct atgtggtatt ctttcttcgc
     3481 gcaacgatcg tccacaggcc gttcaaccct gcaa
//
LOCUS       DATIPF010000178         2776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_830403, whole genome shotgun sequence.
ACCESSION   DATIPF010000178 DATIPF010000000
VERSION     DATIPF010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830403"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /gene="purH"
                     /locus_tag="VLL06_04585"
     CDS             complement(<1..574)
                     /gene="purH"
                     /locus_tag="VLL06_04585"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249103.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HSS30275.1"
                     /translation="MAGIKRALISVSDKTGVVEMAKGIEALGAEILSTGGTAKALREA
                     GIKVTDVAAYTGSPEILDGRVKTLHPKIHGGLLGRRSVPAHVEQMAQQGIGPIDVVVV
                     NLYPFEETIAKPHCSFEEAIENIDIGGPSMLRSAAKNHGDVLVVVDPIDYQRVLEAVK
                     AGTVTHELRRELAMKVFQHTARYDSLIAGYL"
     gene            complement(778..987)
                     /locus_tag="VLL06_04590"
     CDS             complement(778..987)
                     /locus_tag="VLL06_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30276.1"
                     /translation="MTIGTVAIGQLVDLTFLAELKALPTEPVGSIGLIGAVTHAHEDV
                     MRRGKQSIGIGQSKYVRSNAHGDWP"
     gene            complement(1006..>2776)
                     /locus_tag="VLL06_04595"
     CDS             complement(1006..>2776)
                     /locus_tag="VLL06_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30277.1"
                     /translation="VADERAGEVEGIIVSERACDRRSQWFGIPETPDDISCSTKSRPL
                     YKVLTYTNEKDLYGDPEDRDKDLIDRLLVRVKDTRTDRMRNARFLVLGYEIPWDEDHT
                     LIRYGIRFYFIDTFDLEASAKDERATTTEYLGYELDYANEATPLPILAHSMKDGVFEA
                     SRDLYKNKQEYERKIAAERTPAGELSTGRRLLYWTGYPFAVAIGVKNALFEVAKMPFS
                     TVAGLLSGRDNPLFYPAQNLRTAWNTLEVELGHEPYYYFLPGLMNLIGELPFVGQVFQ
                     FNAGPKHLDWDKAPEPGRIRDKIFLSRGIYGGDRWGQDTGLWTMWTRAAYPDFDIYSP
                     PYRHGTITDVLWSMFNLSHGPAYNEAAYIMRHAGQADRLYLSGHSGGVQRSASASRIL
                     ANHGYSPQKVFGIAGPNVGQAYVDPRFPRAFHIFLNADTVGDKDVVSKIGVVAGAYAS
                     ALDSIVLGPPKYLCGGLAGVFYNRAQAWCYDVFDHLGSSNSTISPIRGKIATLHQTPF
                     RLSLAEPIVFDAYIRSEFATAFRQDLERPSTDMEFLKSLTNQWFGLDYWALPGTKQGK
                     EEHDSAHYKEERIGAIPWQRIGH"
BASE COUNT          634 a          802 c          704 g          636 t
ORIGIN      
        1 cgagatatcc ggcgatcaga ctgtcgtagc gcgccgtgtg ttggaagact ttcatcgcca
       61 gctctcgtct caactcatga gtcactgtcc ccgccttcac cgcctcaagc acccgttggt
      121 agtcgatcgg atccaccacc accaagacat cgccatggtt tttggcggca gagcgtagca
      181 tcgacggccc accgatatcg atgttttcaa tggcttcttc aaaggaacag tggggtttcg
      241 cgatcgtctc ctcgaacgga tagaggttga ccacgaccac atcgatcggt ccaattccct
      301 gctgtgccat ctgctcgaca tgggcaggta ccgaacgccg tcccagtaaa ccgccatgaa
      361 tcttggggtg aagcgtcttc acccggccgt ccagaatttc cggtgagccg gtataggccg
      421 ccacgtcggt gaccttgatc ccagcctccc gcaatgcttt ggccgttcct ccggtcgaaa
      481 ggatctctgc gccaagcgcc tcgatcccct tcgccatctc caccacacca gtcttgtcag
      541 aaacactgat cagcgcccgc ttgatgccgg ccatggttca actccttggg tccactaaaa
      601 tggaaaggtg aaattcaact cgtgaaaggt gggcgaagaa taacaaatgg tctgggtaag
      661 tgcaagaaca ggaatgagca acactcaatc agcttgcggg acactgtgag gaacgaacac
      721 tcccattttg gctaggccat cctccttcaa agagtttaac tttacgtcac acgaccacta
      781 tggccagtca ccatgtgcat tcgaccgaac gtatttcgat tggccaattc caatactttg
      841 cttgcctcgt ctcattacat cctcatgggc atgcgtcact gctccaatta gtcctatcga
      901 gccgacaggc tccgtcggca acgctttcaa ctccgccaag aatgtcagat caaccagttg
      961 tccaatcgcg accgtcccga tggtcatacc acgaagatcg acctatcaat gcccgattcg
     1021 ttgccaggga atggccccta tccgctcttc cttatagtgt gcagaatcat gctcctcttt
     1081 cccctgcttt gttcccggca acgcccagta gtcgagcccg aaccactgat tcgtgagact
     1141 cttcaaaaat tccatgtcgg tgctggggcg ttcgagatct tggcggaatg ccgtagcaaa
     1201 ttcgcttctg atatatgcat cgaacacgat tggttcggca agtgacagcc tgaatggggt
     1261 ttgatgaagc gtggcgatct tcccgcgtat cggcgagatc gtggaattgg acgaacccaa
     1321 atgatcgaac acatcgtaac accatgcctg cgcgcgattg tagaacacac ctgcaaggcc
     1381 tccacagaga tatttcggcg gcccgaggac aatcgaatcc aacgcgctgg cgtatgcgcc
     1441 ggccacgaca ccgatcttcg agaccacgtc cttgtcgccg acggtgtcgg cgtttaagaa
     1501 gatatgaaat gcacgcggga agcgcggatc gacatatgcc tggccgacat tcggaccggc
     1561 aataccaaag actttctgcg gagaataccc gtggttcgca agaatacgtg atgcactggc
     1621 gcttcgctgc acgccgccgc tgtgcccgga aagatagagc cgatcggctt gccctgcgtg
     1681 acgcatgata taggcggcct cgttataggc agggccatgc gacaggttga acatggacca
     1741 gaggacgtca gtaatagttc catggcggta gggaggacta tagatgtcaa agtccggata
     1801 ggcggctctg gtccacatgg tccagagtcc ggtatcctgc ccccatctgt ctccgccata
     1861 gatccctcgc gaaagaaaga tcttgtcacg gatccgtcct ggttccggcg ccttgtccca
     1921 atccaaatgc ttggggccgg cattgaactg aaatacttgg ccgacgaacg gtaattcgcc
     1981 aatgagattc atcaagcctg gcaaaaaata atagtacggt tcgtgcccga gctccacctc
     2041 gagcgtattc cacgccgtcc gtaaattttg cgccggataa aacagcgggt tgtctctccc
     2101 ggacagtaag ccggcaacgg tactgaaggg catcttcgcc acctcgaaca gggcattctt
     2161 gacgccgatc gcaacggcga aggggtaccc ggtccagtag agtaagcgcc gaccagtgga
     2221 aagctcgcct gcgggagtcc gttccgctgc gattttgcgt tcgtattcct gcttgttttt
     2281 gtaaaggtcc ctcgatgctt cgaatacacc atctttcatg ctgtgcgcaa gaataggcaa
     2341 cggcgtcgcc tcattggcat agtccagctc atagccgagg tattcggtgg tcgtcgcccg
     2401 ctcatctttg gcgctcgcct caagatcgaa cgtatcgata aaataaaacc gaattccgta
     2461 gcgaatcagg gtatgatctt catcccatgg aatttcgtac cccaatacga gaaatcgagc
     2521 attgcgcatc cgatcagtgc gtgtatcttt gactctgacg agcagtcggt cgatcagatc
     2581 cttatcgcgg tcttctggat cgccatagag atccttttcg ttcgtatagg taagcacttt
     2641 gtacaagggt ctcgacttgg tcgaacatga aatatcatcg ggagtttcag gaatgccgaa
     2701 ccattgcgat cggcggtcac aggccctctc agagacgatg atcccttcaa cttctcccgc
     2761 cctctcatct gcaacc
//
LOCUS       DATIPF010000179         6213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_652948, whole genome shotgun sequence.
ACCESSION   DATIPF010000179 DATIPF010000000
VERSION     DATIPF010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652948"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..494
                     /locus_tag="VLL06_04600"
     CDS             264..494
                     /locus_tag="VLL06_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30278.1"
                     /translation="MDMMGRVGLVEIPILIAPDQKVWMDRMIKEGKIAIPPGGTLEKG
                     SLYSMFVRMLLHNAMEEQKRQEVLDAEDEDDE"
     gene            complement(753..2036)
                     /gene="hemL"
                     /locus_tag="VLL06_04605"
     CDS             complement(753..2036)
                     /gene="hemL"
                     /locus_tag="VLL06_04605"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HSS30279.1"
                     /translation="MKTTRSAKLFAEAQQLIPGGVNSPVRAFRSVGGQPRFIKRAKGS
                     RLYDVDGNTYIDYVLSWGPMILGHAAPSVVSTIKTAAANGTSYGAPTELEVVLARMIH
                     QAFPSMEKVRLVSSGTEAVMSAIRVARGFTKRDSILKFEGCYHGHSDYLLAKAGSGLA
                     TLGIPDSLGVPADFAKHTLTVPYNDIRAVQQIVKEHRNMLACVMLEPIAANMGVVPPA
                     PEFLSSLRRLTAENGILLIFDEVISGFRVTYGGAQTLYGITPDLTVLGKIIGGGLPVG
                     AYGGRKEIMDLIAPLGPVYQAGTLSGNPLAVSAGIETLRQLKARGVYKKLEEKSAALA
                     KGIGDAAKKAGVPLTQTRVGSMLGAFFTSGSVVDWSSAKLSDVKRYGQFFHQMLEQGV
                     YLAPSQFEAAFLSTAHSSRDIEHTIKATHSAMKDL"
     gene            2303..2542
                     /locus_tag="VLL06_04610"
     CDS             2303..2542
                     /locus_tag="VLL06_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30280.1"
                     /translation="MKIICSWCRQEGKSELVGEKAPFDDARETHGICVAHRGAVEARW
                     QASIHTAAHTESVSGLSSVLFRWTGLLNVTKKMRQ"
     gene            complement(2637..3194)
                     /locus_tag="VLL06_04615"
     CDS             complement(2637..3194)
                     /locus_tag="VLL06_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HSS30281.1"
                     /translation="MTPKHTPTLTAYLQSTKAPWTTLLILTAGQRTESLVRPPTGYAL
                     KVIKGRQCKTPAHLFAEFAQVLKFPDYFGYNWDALEECLADLEWLPAKGYILLITEAG
                     HVLPDDEEEFETFLEILRDAGEAWGNGQAGMSTQRATPFHVLFAVSEQEKAKRAHWGM
                     KDISVEPTRGANTQQRSPKRRSSPH"
     gene            3667..5991
                     /locus_tag="VLL06_04620"
     CDS             3667..5991
                     /locus_tag="VLL06_04620"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HSS30282.1"
                     /translation="MESAMDRLEFVTVETLLAYGEMLARRTPSEGIALPPIPTSSVDR
                     PQRTRNAMEKIRAFLQSAQGGFSGAEEYRSTRRALIAEGCQGDELVFYAAWNTLLAAG
                     ELAPLFRVPIGSVQKPTHRRPVAIVPRAQLTPQLVEDRIVLDLGEGRFWLLPRDLTGR
                     TLLFTMRHGISRVESGTYRVGCRLANVLDPERGVPKADAVGAALARMLGVVGQQLDFL
                     RIHNYLNPCTFVHLVSQSPNTRQLYERVISALMQAQVHHTEALPPACDPALDSQDFGW
                     VTGLEKKVEAEEAAKAFGVDVQTAKRLIKHPFYSYPGGHSFFDVYVDIVDGFRRLGQS
                     HQGKAVCLYSHSSTLRALMIYLDPRPFREAFTEFGEYKEGQDNVVLLTYEQGLLSGYS
                     TAVGLSARELAARETWVKVERERRDWVTLKPRQIKRIVALVSGGDFAGAGAALKELCV
                     TGHRFGLEVYFARHGFLGLANNWIEAVTEDDTRGMGSHASSPIGSSRFEDFKDEQVQQ
                     ATMRHLRPYMEDGALVVIGGDGSLRGARALYEGFGVQPVGIPGTIDNNLAGTTSLGFH
                     SAVSLANQSIESLKATSSAMGSVFFVEIMGAGSGHLALACAYQARAEGLLVNEHPDPD
                     AYIDDFILGTLKRTLGVPNKSHLFVVAEQTPHRHHPDGGVRGLVEYVASTLTTWPQFQ
                     ACPGEYRLASATKATILGHTLRGAPPTPEDKTIGQDLAYEAIRRLVKEPERVVGCMLA
                     YRGTGTIEVIPLHAVAPKQFDWEIFARMHGSEMP"
BASE COUNT         1339 a         1666 c         1809 g         1399 t
ORIGIN      
        1 aatgatgaag gtggggcagt cgatggttgg gccgtaacgt gatgagggaa gctcaggatg
       61 tagccaacag caatgagtat accgaacgtc atgtgacttt gtgccatagc cttcgcggcg
      121 ctgcacacgt catgccagat cagtcgagtt gacaatacaa aaatccgcgt gctgcaatgg
      181 caacgtctct cgtcgttcgc gagggacggc ttacgagttc tgcgttacga tttcacagag
      241 ggttctattt cacggaggtt tctatggata tgatgggacg tgtggggctg gttgagattc
      301 cgatcttgat tgcgcccgat caaaaagtct ggatggaccg gatgattaag gaaggcaaga
      361 ttgctattcc tccaggtggg acgctggaaa aaggttcgct ctattcgatg tttgtccgga
      421 tgctcttgca taatgcgatg gaagagcaga agcgccagga agtattagat gcagaggacg
      481 aggacgacga atagggaggc tgctcaaaac gaccgcccat ctcacccggt caaccctggc
      541 gattacctca cccgcccgcc ctgagactgc cgagacagcc tctttgccca gggccgcgcc
      601 ttggcccggg cttcgttctg atttcgctca tggcctcaac gtactcgcgt ccgcctcagc
      661 ctttcgcact tgctggaagg ctgctttgag cagaggtcaa ctctgtatct caacagattc
      721 aagttgtatc cggcgagata cgagcgatac gattagaggt ccttcatcgc agaatgcgtg
      781 gctttgatcg tgtgttcgat gtcgcggctt gaatgcgcag ttgagaggaa ggcggcttcg
      841 aattgagagg gggccaagta taccccctgc tccaacatct ggtgaaagaa ttgcccatat
      901 cgtttcacgt ctgacagttt tgcgctactc caatccacca cagagcccga cgtaaagaaa
      961 gcaccgagca tcgaaccgac tctggtttgc gtgagcggca cgccggcttt cttagccgcg
     1021 tcaccaatcc ccttggccag agctgctgat ttctcctcca gtttcttgta gacgcctcgg
     1081 gctttgagct gcctgagcgt ttcaatgccg gcggatacgg ctaacggatt tcccgacaat
     1141 gttcccgcct gatagactgg ccccaacggc gcgatcagat ccatgatctc ttttcgccct
     1201 ccgtaggcgc cgaccggcaa tcctccaccg atgatttttc ccagcaccgt cagatccggg
     1261 gtgatgccat agagtgtttg ggctccaccg taggtcacac ggaagccgga gatgacctcg
     1321 tcgaagatca gaaggattcc attttctgcg gtgagtcgac gaagtgatga aagaaattcc
     1381 ggagccggcg gcacgacgcc catgttcgcg gcaatcggtt ccagcatcac gcaggccaac
     1441 atgtttcgat gttccttgac gatctgttga accgctcgga tgtcgttgta tggaacggtc
     1501 aatgtgtgct tcgcaaagtc agccggcact ccaagcgagt ccgggatccc caacgtggcc
     1561 agaccggaac cggccttcgc caataagtaa tcgctgtggc cgtgatagca tccttcaaat
     1621 ttcagaatac tgtctcgctt cgtaaagcca cgggcaaccc gaatcgcgct cataaccgcc
     1681 tcggtgccgg agctgacaag ccggactttc tccatcgacg ggaaggcctg gtggatcatg
     1741 cgggcgagaa cgacttctag ctcagtcggt gcgccgtagc tcgttccgtt tgcggcagcc
     1801 gttttgatcg tactgacgac cgatggggcg gcatgaccca agatcatcgg gccccatgat
     1861 agtacgtagt cgatgtaggt attcccatcg acgtcgtaga ggcgtgagcc tttggcacgc
     1921 ttgataaacc ggggttggcc tccaacggag cgaaaggcgc gcaccggact attcacccct
     1981 ccgggaatga gctgctgagc ttctgcaaaa agcttggcag agcgtgtggt tttcataatg
     2041 ggggcgcaca gtaccatgct gccgagcctc ggtcaagaaa gagaaggctc atgctgtgcg
     2101 ataaggcgac tgtggcgaaa aagtgtctga agagatacat atctcagcaa ttttgataca
     2161 gactattttc ctgggacgca gggagatttc gtaatccctt ggaaatgtgc tgattcatgc
     2221 tactcatcga gtgcggcttg agtcttgcct ggggaagcat ggactattca aagttctaga
     2281 aaacgattat aagtgatcga tcatgaagat tatttgttcg tggtgccgtc aagaggggaa
     2341 atcagagttg gtgggcgaga aggcgccttt cgacgatgca cgggagacac acgggatttg
     2401 cgtcgcccat cgcggtgcag tcgaggctcg ctggcaagca tcgattcata ccgccgcaca
     2461 cactgaatcc gtgagtggat tgtcttctgt gctcttccgc tggacaggct tactgaatgt
     2521 gaccaagaag atgcgtcagt agatccgtag gatagacgca tcaccttgtc gatttgccga
     2581 gagagacatt cccattctcg cgtgaatcac cttgaacagc tagtcgatct tgccggctag
     2641 tggggcgaag atctgcgctt gggagatcgc tgttgcgtat tcgcaccccg ggtcggttcc
     2701 acgctgatgt ctttcatccc ccaatgggct cgtttagctt tttcctgttc cgatacagcg
     2761 aacaacacgt gaaagggcgt ggctcgttgt gtgctcattc ctgcctgtcc gtttccccag
     2821 gcctctcccg catcgcgcaa tatctcaaga aaggtttcaa actcttcttc gtcatctggc
     2881 aatacatgac ccgcctcggt gatcaataag atatagcctt tggcaggcag ccattccaga
     2941 tcggccagac actcctccaa ggcatcccag ttgtagccaa agtagtccgg gaacttcaaa
     3001 acctgtgcaa actccgcaaa cagatgtgcc ggagtcttgc actgacggcc cttgatgacc
     3061 ttcagcgcat atccggtcgg tgggcgcacc aacgattcag tgcgctgtcc ggctgtgaga
     3121 atcagcaagg tggtccatgg ggccttcgtc gattggagat aggcggttaa cgtcggtgtg
     3181 tgtttcggag tcatggtatc cgttctgtat accgagagcc gccgagtatt ttaggattca
     3241 catcgtactc gcgatagtga cgacgaagca gccatcttcc ggccctgcgg actgaacgga
     3301 tgggagagag gcggacggta cggtcggttc agacgatgag ccggcgttga gggaatctgt
     3361 gggactgccg aacagtagcc caacaaggac gacggatatt ccataaatct tccaagttcg
     3421 atccatagtt tcgcgttcgt atcacgagat gaagcagcat agtacgaacc ctagcacgca
     3481 cgatctcaga ggatcaacgg ttgtaagagg agcgggcgtt cgaggtcagg agttattgct
     3541 atcgcgcgtt cgccgggcgt agcatccaat gtactcaact tgccgaagat gttctcctgt
     3601 ccggtatctt tcaaccatgg ctagcacgat ggtcgcttat gctaggacaa gtcaacgaga
     3661 tacagattgg aatccgctat ggaccggctt gagtttgtga cagtcgaaac gctcttggcc
     3721 tatggagaga tgctggcgcg tcgcaccccc agtgagggaa ttgcactccc acctatcccg
     3781 acctcctcgg tcgatcgacc acagcgaacc cgtaatgcca tggagaagat cagagcgttt
     3841 ctacagtctg cacaaggggg cttttctggg gcagaagagt atcgatctac ccgtcgcgcg
     3901 ctgattgcag agggttgcca gggcgatgaa ctggtgttct atgccgcctg gaacactctc
     3961 ctcgcagcag gcgagttggc cccgcttttc cgcgtgccaa ttgggtcagt gcagaaacca
     4021 acgcatcgtc ggccggtagc gatcgtgccg cgtgcgcaac tgacgccgca gttggtcgag
     4081 gatcgaatcg tgctggactt gggcgaaggt cggttttggc tcttaccacg agatttaacc
     4141 ggtcgcacgc ttctgttcac gatgcgtcat ggaatctcac gcgtggagag cggcacctat
     4201 cgagtcggat gccgcctcgc aaacgtgctg gatcctgagc gaggcgtccc taaagccgat
     4261 gcggtcggag cagccctggc acggatgctg ggcgtggttg ggcaacaatt ggacttcctg
     4321 cgcattcaca actacctcaa tccgtgcacc ttcgtccatc tggtgagcca gagccccaat
     4381 acacggcaac tgtatgagcg ggtgatttcg gcgctgatgc aggcgcaggt gcaccatact
     4441 gaggcactcc cacccgcttg cgatcccgcc ttagactccc aagatttcgg atgggtcaca
     4501 gggctggaga agaaggtgga agccgaggaa gccgcaaagg catttggcgt ggacgtgcag
     4561 acagccaagc gcctgatcaa acatcccttc tacagctatc ctggtgggca ttccttcttt
     4621 gatgtctatg tggatattgt cgatggtttc cgtcggctgg gtcagtccca ccagggaaag
     4681 gccgtgtgtc tgtacagcca tagttccacg ctgcgggcgc tcatgattta tctcgatcct
     4741 cgcccctttc gtgaggcctt caccgagttt ggtgaatata aggagggaca ggataacgtc
     4801 gtgttgctga cctacgaaca ggggctctta tccggctatt ccaccgctgt gggtctgtca
     4861 gcgcgggaac tggcggcgcg ggaaacctgg gtgaaggtcg agcgcgagcg cagagactgg
     4921 gtcacgctca agccccgaca gattaagcgg atcgtggcat tggtctcggg tggcgatttc
     4981 gctggtgctg gcgccgcatt gaaagaactg tgcgtgacag gacacaggtt tggcctggaa
     5041 gtctacttcg cccgtcatgg gttcttaggt ctggccaaca actggattga agcggtgacc
     5101 gaggacgata cccgcggcat gggcagccat gcgagcagtc cgattgggag cagtcgcttc
     5161 gaagatttca aagacgagca ggtgcagcag gccaccatgc ggcatcttcg gccctatatg
     5221 gaggatggag ccttggttgt cattggtggg gacgggagtc ttcgaggtgc gagggcgctc
     5281 tacgagggat ttggggtcca gccagtaggg attccaggca cgatcgacaa taatctcgca
     5341 gggacgacct ccctgggttt tcattcggcc gtgtctctgg cgaatcaatc catcgagtcg
     5401 ctgaaagcaa cgagttccgc catgggcagt gtctttttcg tcgagattat gggggcgggg
     5461 tctggccacc ttgccctcgc ctgtgcgtat caagccagag ctgaaggtct cttggtcaac
     5521 gagcatcccg atcccgacgc gtatatcgac gacttcatcc tgggcacgct caagcgcacc
     5581 ttgggcgtcc cgaacaaaag ccatctgttt gtggttgcag aacagacacc ccatcgccat
     5641 catccggatg gcggcgtgcg tggtcttgtc gagtatgtcg ccagcacact caccacctgg
     5701 ccacaatttc aggcatgtcc cggtgaatat cgcctcgcct ctgcgaccaa agccacgatc
     5761 ctcggccaca cattacgggg ggcgcccccg actcccgagg acaagaccat cggacaagat
     5821 cttgcctatg aagcgattcg gcgtctcgtc aaggagccgg agcgggtggt cggatgcatg
     5881 ctggcctatc gcgggacagg caccatcgag gtgatcccac tccacgctgt ggcaccgaag
     5941 cagttcgact gggagatctt cgccaggatg catgggagcg agatgccgta gcagcgtgaa
     6001 ttgttcattg cgtctcactg tgaaatcctt gccctgatgc catttctact caccgtcaca
     6061 gggccattca atcatggatt aacctggcgg cctgcacctg aaatgactgt tgcgttgtgg
     6121 gcatggaagt cgtttgctag aatggcacgt ctgaacatcc tcgcgacatc ctcgcgagaa
     6181 aaacagaaaa agctggacgc acggaaaatg cgg
//
LOCUS       DATIPF010000180         2806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1184578, whole genome shotgun sequence.
ACCESSION   DATIPF010000180 DATIPF010000000
VERSION     DATIPF010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184578"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(331..2001)
                     /locus_tag="VLL06_04625"
     CDS             complement(331..2001)
                     /locus_tag="VLL06_04625"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HSS30283.1"
                     /translation="MPDWSKGTIMKGSDLLLRCLENEGVQWIFGVPGEETLDVLNSLV
                     DSQIRFIAARHEQGAAFMADVHGRLTGKAGVCLSTLGPGATNLLTGVADAYLDRAPLV
                     AITGQASLDRMHKESHQFIDVLSMFKPVTKWNAEIFHPRTMAETVRKAFKVAQTEKPG
                     STHLELPEDVAAETVRDAEVDGPLRVQAPFLTEPLARQVSRAVEVLATARRPVLLVGN
                     GVIRGQAHEAVRRFARQANMPVLHTFMSKGVVPDSDPLSCHTIGLQMRDYPAMVIEQS
                     DVVVAVGYDFVEYAPCLWNPNRDKHIVHVDVSPAEVDAHYIIDVGVLGDIALSLDQIG
                     DQLAPFDHSWAQESRGRVQTAYEQEWAQPSGWPLRPQHIIRDLRQALASDDLVICDVG
                     AHKLWMARMFPCEAPNTCVISNGFASMGMALPGAVSAKLLFPSRRVVAVTGDGGYLMN
                     VQELETAVRLQLPLVVLIWRDNGYGVIRWKQQMKFGRTSGVEFSNPDFVALAQSFGAV
                     GYRVEGPSELGPVLNDALLCGKPAVIDCPVDYSENLRLSERLKVLGNT"
     gene            complement(2130..>2806)
                     /locus_tag="VLL06_04630"
     CDS             complement(2130..>2806)
                     /locus_tag="VLL06_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSS30284.1"
                     /translation="FLFDVDNTLLDNDRVTADLKHHLEQTVGNGRQENYWAIFERLRA
                     ELGYADYLGALQRYRIEHPRDPQVLTVSHFLVNYSFANRVFPNAIDAVEHVKQWGKAV
                     ILSDGDVVFQPRKIERSGLFEAVEGQVLIYIHKEQELDDVERRYPGDHYVLIDDKLKI
                     LTAVKKVWGARVTTVFPRQGHYARDPQLLKSYPPADVNIDRIGELLRYDLPQLVGAGD
                     TSARDR"
BASE COUNT          593 a          894 c          731 g          588 t
ORIGIN      
        1 tcgcatgcac agccttctcc tgcttgcccg tataatagga cgagcagccg acaagcggta
       61 cgaatgccgc cgccacgagc gcagttccca caagtgtacg attcatagat cctccttggt
      121 tgtccaattc gccgaccaga ccgtcgcgtg tattgagggc aggtacgacg agggcggtta
      181 cgttggaaca tcatgcggga gtgcatcgct gttatgcaac acgtgcggac tctcgaagta
      241 ctcctgtgcc ggagtggttt ttccacagcc tgctgaaccc tgcgtagact tcaatctgcc
      301 ataccccttt tcatcctcga ataagcttcc tcatgtgttt cctagcactt tgagtcgttc
      361 cgatagacga agattctcac tgtaatccac cgggcaatcg atcacggccg gctttccgca
      421 caagagcgca tcgttcagca ctggccccag ttccgacggt ccctcaacac ggtagccaac
      481 cgctccaaaa ctttgcgcca aggcgacgaa gtccggatta ctgaattcca caccggaagt
      541 ccttccgaac ttcatctgct gtttccatcg aatgaccccg tagccattgt cccgccaaat
      601 caacacgacg agtggaagct gcagtctcac ggcagtctcc agttcctgaa cattcatcag
      661 ataccctccg tctccggtca cagccacgac acgccgtgag ggaaacagga gcttggccga
      721 aacggccccg ggcagcgcca tgcccatcga cgcgaatccg ttcgaaataa cgcaggtgtt
      781 cggcgcctcg cagggaaaca tccgggccat ccataactta tgcgccccca cgtcgcagat
      841 gaccagatcg tccgatgcca acgcctgtcg caaatcacga atgatgtgct gaggtcgcag
      901 aggccaccca gacggctggg cccattcctg ttcgtatgcg gtctgcacgc gccccctgct
      961 ttcttgggcc cagctgtgat cgaacggagc aagctgatcg ccgatctggt cgagggacaa
     1021 ggcgatatcc cccaagacgc cgacatcaat aatatagtgc gcatccactt cggccggcga
     1081 cacgtccaca tgcacgatat gcttgtcgcg gttcgggttc cacaagcagg gggcgtactc
     1141 cacaaaatcg taaccgaccg cgacaacgac atcactctgc tcgatcacca tagccggata
     1201 atcccgcatc tgtagcccaa tcgtatgaca cgacaacgga tcgctatctg gaacgacacc
     1261 cttgctcatg aacgtgtgaa gtaccggcat gtttgcctgt ctggcaaacc gccggaccgc
     1321 ctcatgcgcc tgtcctctga tcaccccgtt cccgaccaag aggaccggcc gtcgtgccgt
     1381 ggccaacacc tcgacagccc ggctcacttg ccgcgccagt ggctccgtca agaacggcgc
     1441 ctggacgcgc aacggcccat ccacttcagc atcacgcacc gtttccgccg caacatcctc
     1501 gggcaactcc aaatgggtcg agccgggttt ttcggtctgc gcgaccttga acgctttgcg
     1561 caccgtctcg gccatggtcc gaggatgaaa gatctcggcg ttccacttcg tgactggttt
     1621 gaacatggac aagacatcga tgaactgatg cgactctttg tgcattcgat ccagcgaagc
     1681 ttgtcccgtg atggcaacca gcggggcgcg atccagatac gcgtctgcca cgccggtgag
     1741 taaattggtt gcaccaggcc ccaaggtgga gagacagacc ccggcctttc cggtcagccg
     1801 cccatggaca tcggccatga aggccgcgcc ttgctcgtgc cgtgcagcaa tgaatcggat
     1861 ctgcgaatcc acgagcgagt tcaagacgtc cagcgtctcc tctccaggta cgccgaagat
     1921 ccactgcacc ccctcatttt ccagacaacg cagcagtaag tccgaccctt tcatgatagt
     1981 ccccttgctc caatcaggca tggctgccga cctgtcctcc gcactctcat ggtggcttgg
     2041 tgggccatct cccctctggc aattccatca ggcggacaac cgtaaggttg tggaggcaac
     2101 gttcggcttg gtcccatacc gcaagatcat catcggtcac gagcggaggt gtcgcctgcg
     2161 ccgacaagct gaggaagatc gtaccggagt agctcgccga tgcggtcgat attcacgtcc
     2221 gccggcgggt aactcttgag cagctgtgga tcgcgcgcat agtgtccttg ccgtgggaac
     2281 acggtcgtca cccgcgcgcc ccagaccttc ttgaccgccg tgaggatttt gagtttgtcg
     2341 tcgatgagca catagtgatc ccccggatac cgccgctcga catcgtcgag ttcctgctct
     2401 ttgtggatgt agatcagcac ctggccctct acagcctcga agagaccaga ccgttcaatt
     2461 ttcctcggct ggaacactac atccccatcc gacaagatga ctgccttccc ccattgcttg
     2521 acgtgctcca ccgcatcgat cgcgtttggg aacactcgat tcgcaaatga atagttcacg
     2581 agaaagtgag agaccgttaa tacttgtgga tcgcggggat gttcgatccg atagcgctgg
     2641 agcgccccca agtagtctgc gtagccgagt tccgctcgta accgttcgaa gatcgcccag
     2701 tagttctcct gccgtccgtt ccccacggtc tgttcaagat gatgtttgag atcggccgtc
     2761 acccgatcgt tgtcgaggag ggtgttatcg acgtcaaaga gaaaca
//
LOCUS       DATIPF010000181         4158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1626569, whole genome shotgun sequence.
ACCESSION   DATIPF010000181 DATIPF010000000
VERSION     DATIPF010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626569"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..395)
                     /gene="fliE"
                     /locus_tag="VLL06_04635"
     CDS             complement(90..395)
                     /gene="fliE"
                     /locus_tag="VLL06_04635"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HSS30285.1"
                     /translation="MDQIKAITMPTVPLVDNVAASQATAGAHGTGFMNSLQQAISKAN
                     DLQLEANHATEALMTGQTQNIHQTMVALQEADVSFQLMMQIRNKLVTAYEEIQRMQI"
     gene            complement(398..847)
                     /gene="flgC"
                     /locus_tag="VLL06_04640"
     CDS             complement(398..847)
                     /gene="flgC"
                     /locus_tag="VLL06_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HSS30286.1"
                     /translation="MEMTDSMAVSVTALDAQRQRLNVIASNLANAQSTHTPGGGPYKR
                     RDVVFQSTPVASPFQRTFRQVSTGPGAHALDGVKVLRVKEDTRPGQVMYDPRHPDADK
                     KGFVKMPNINVMEEMVNMIGASRSYEANVQAINATRAMWNKALEIGR"
     gene            complement(922..1329)
                     /gene="flgB"
                     /locus_tag="VLL06_04645"
     CDS             complement(922..1329)
                     /gene="flgB"
                     /locus_tag="VLL06_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HSS30287.1"
                     /translation="MTIFDRTMQLLHRTLDLRQARQRVIASNIANEETPGYRAADLSF
                     QDSLQAAHRGRGPVMLAVTQGRHIGPRGEGFQRVVGKLDPVPAGDLPLDANSVNIELE
                     MAKMSDNAMQFNSAASIMAIRFRQLMSAIRDAR"
     gene            complement(1502..2908)
                     /locus_tag="VLL06_04650"
     CDS             complement(1502..2908)
                     /locus_tag="VLL06_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSS30288.1"
                     /translation="MNEYDAVSLVADVPAQAERILIVDDDPSMRTALMESVRRLGYDV
                     QGAIDGADAVERVGRSKPWLVVTDLKMPRLGGLDLIKEIKSRSPQTLMVLMTAYGTVE
                     TAVEAMKFGASDYILKPFSTDLLERVIANLKARGADASASSPAPIDQRSILTQDPGMA
                     RLLSTVEGVAASQATILINGESGTGKELLARFIHSRSPRAHRPFIAVNCAALPDGLLE
                     SELFGHERGAFTGAIMKKIGKFEMAHTGTVLLDEISEMNMGLQAKLLRVIQEREVDRV
                     GGREPVSVNIRVIATTNRSLYHEVEQGRFREDLYYRLNVFPITLPALRERPADIPLLA
                     RHFVAVSAARNGLPLPTVSVEAYAMLQDRRWKGNVRELENVMERAVLVAGNEPILPCH
                     LLLESGGQPVPPVPVSPMPQPSGNGSLWEMERELIFQTLARVRENRTHAAKELGISIR
                     TLRNKLREYRGTNCRASV"
     gene            complement(2905..>4158)
                     /locus_tag="VLL06_04655"
     CDS             complement(2905..>4158)
                     /locus_tag="VLL06_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS30289.1"
                     /translation="AFRSFDDAAATLQQSYQTLASRLEQLDVELADRNEALGQNLRAN
                     EELREHLTAIVESLSTGLLVMDTEGTITRCNQAGEQLLGLTREEIMGRSLAACLKEAQ
                     LDGDVYPLTTQHGATVALSQRALQSSEGRHAGSLVLIQDVTAVRELEDRLQRRNRLAA
                     MGEMVGRIAHEIRNPLGSIELFASMLRRDLNGVPALRGYAEHISSSVHMMNRLLTNLL
                     LYTKPDCSHGKWHETEALMLDALTLAAHAVANTRIDIRLQVDPLAARIWCDAGQMKQV
                     LLNLILNSIQAMPGSGTLTLSATVTEEQISGEQAFRLSVSDTGSGIPAEQRSRIFDPF
                     FTTKDEGTGLGLAIVHAIIEAHRGRIDVESAEGRGTIFTIALPHHREADRVGTPECPA
                     PNRGDRTGTSPMVGERHFIEEETSA"
BASE COUNT          870 a         1261 c         1091 g          936 t
ORIGIN      
        1 gtctgatcag tttcgtccaa gcaatcacac gagatggacc agatagacga gacagacctg
       61 atagaccaga ggactttttc agcatccgac taaatttgca ttcgctggat ttcttcatac
      121 gccgtcacca gtttgttcct gatctgcatc atcaactgga acgacacatc ggcttcttgc
      181 aacgccacca tcgtctggtg gatgttctga gtctggccgg tcatcaacgc ctcggtcgca
      241 tggttcgctt ccaactgaag gtcgttggcc ttactgatcg cctgctgcag cgaattcatg
      301 aatccggttc cgtgagcacc agcagtggcc tgtgaagcgg cgacgttatc caccaacggc
      361 acggttggca tggtgatcgc tttgatctga tccacggcta cctcccgatc tccagcgcct
      421 tattccacat ggcgcgggtc gcattaatcg cctgcacgtt ggcttcgtat gaacgggatg
      481 ccccgatcat gttcaccatt tcttccatga cattgatgtt cggcattttg acgaatccct
      541 tcttgtccgc gtccggatgg cgcgggtcat acatcacctg tccgggcctt gtatcttcct
      601 tcacgcgcaa caccttgaca ccatcgaggg cgtgagcccc tggtcccgtc gacacctgcc
      661 gaaacgtccg ctggaacgga ctggcgaccg gagtggattg gaacaccacg tcccggcgct
      721 tgtacggacc acctcccggc gtatgtgtcg actgtgcgtt ggccaggttg ctggcgatga
      781 cattgagccg ttgacgttgt gcatcgagag cggtcacgga caccgccatg ctgtcggtca
      841 tttccatggg cacctccttg ctgtcagcga tcagttgtca gccatcagca tgactgacac
      901 ctgaagcctg acggctattg attatctcgc atcacggatc gcactcatga gctgccgaaa
      961 gcggatcgcc ataatcgacg ccgcactatt gaactgcatc gcgttgtctg acatttttgc
     1021 catttccagt tcgatgttca cggagttggc atccagagga aggtctccag cagggactgg
     1081 gtcgagtttc ccgacgaccc gctgaaatcc ctctccccgt ggaccgatgt gacggccctg
     1141 agtcacggcc agcatcaccg ggcctctccc tcgatgcgcg gcctgcaagg aatcctgaaa
     1201 actaagatcg gcggctcgat atcctggtgt ttcttcgttc gcaatatttg acgcgatcac
     1261 cctctgtctg gcttgtcgta agtccagcgt ccgatggagc aattgcatgg tgcgatcaaa
     1321 aatcgtcata cggctcctcc tctgctctct ctcgcggata ccgatgcaat cagaatgcca
     1381 tcgacggcat gccatgagat gcggtcgcgg gagaaatcgc cgactttctc gtacgtctca
     1441 catctgccga tcggcaaaat ataccgcctg ttttctgccg acatcatgct ggatggccgg
     1501 actatacaga agcccgacaa ttggtccccc gatactctcg aagcttattc cgcagggtac
     1561 gaatgctgat accgagttct ttcgctgcgt gagttcgatt ctccctcact cgggccagtg
     1621 tttgaaaaat gagttcccgc tccatctccc acagcgaccc gttcccggaa ggttgtggca
     1681 tcggtgagac tggaacggga ggcaccggtt gtccgccgga ttccagcaac aagtggcacg
     1741 ggagaatcgg ttcgttaccg gcaacaagca cggcacgttc catgacgttc tccagttcac
     1801 gcacgttccc tttccatcgt ctgtcctgga gcatcgcata ggcttcgacc gagaccgtcg
     1861 gcagcggaag gccattgcgt gctgcggaga ccgctacgaa atgcctcgcg agcaacggga
     1921 tatctgcagg tcgttctctc aaggccggta aggtgatggg aaatacattc aaccggtaat
     1981 acagatcctc ccgaaaccgt ccctgttcta cttcgtggta gagcgagcgg tttgtcgtcg
     2041 caatgactcg aatattgacg gatacaggct cacggcctcc gactcgatcc acttcacgtt
     2101 cttggatcac acggagcaac ttggcctgca accccatgtt catctcactg atttcatcta
     2161 acaacaccgt tccggtatgc gccatctcaa acttcccgat tttcttcatg atggcgccgg
     2221 taaatgctcc tctctcatgg ccgaacagtt cactctccaa gagtccgtcc ggcagggccg
     2281 cgcaattgac ggcaataaac ggtcggtgcg cacggggact cctgctatgg ataaagcggg
     2341 ccagcagttc cttccctgta ccgctttctc cattaataag gatcgtcgct tggctggcgg
     2401 ccacaccctc aaccgtacta agcaagcgag ccatgcccgg gtcttgtgtc aggatagacc
     2461 gctggtcgat cggcgcaggc gatgacgcag aggcatcggc accacgggct ttaagattgg
     2521 caatgactcg ctcgagcaaa tcggtggaga acggcttaag aatgtaatcg ctcgccccga
     2581 atttcatcgc ctctacggca gtctccaccg tcccgtaggc agtcatcagg accatcaacg
     2641 tctgtggcga ccgactcttg atctccttaa tgaggtcgag acccccaagg cgtggcatct
     2701 tcagatcggt caccaccaac cagggtttgg accggccaac ccgttccacg gcatcggcac
     2761 catcgatggc accctgcacg tcataaccaa gacgccgaac tgactccatc aacgcagtcc
     2821 gcatcgacgg atcatcatcg acgatcaaaa ttcgctcggc ttgcgcggga acatccgcca
     2881 caagactgac tgcatcgtat tcgttcatgc ggaggtctcc tcttcaatga agtgacgctc
     2941 gccgaccatg ggagaggtgc cggtccgatc accgcgatta ggagcgggac attcgggcgt
     3001 cccgacacga tccgcctcac gatgatgtgg aagcgcaatc gtaaatatcg tgccgcgccc
     3061 ctcagcgctc tccacgtcga tccggcctcg atgcgcttca atgatcgcgt ggacgatggc
     3121 caatcccaat ccggtccctt cgtccttcgt cgtaaagaat ggatcgaaaa ttcttgagcg
     3181 ctgttccgct ggaatgccgc taccagtatc cgaaaccgaa agacgaaacg cctgctctcc
     3241 ggaaatctgt tcttctgtga cggtcgccga cagcgtcaat gttccactgc cgggcatggc
     3301 ttggatactg ttcagaatca agttcaggag cacttgtttc atttgccccg cgtcacacca
     3361 gattcgagcg gcgagaggat cgacctgcaa acgaatgtcg attctcgtgt tcgcaacggc
     3421 gtgggcagcc agagtcaacg catcgagcat gagggcttcc gtctcatgcc acttcccatg
     3481 ggagcagtcg ggtttggtat agaggagaag attggtcaag agacgattca tcatgtggac
     3541 agacgatgaa atatgctcgg cgtatcctct caatgccggg acaccattca aatcgcgacg
     3601 cagcatcgac gcaaacagtt caatactgcc gaggggattc cgaatttcgt gggcaatacg
     3661 cccgaccatc tcacccatag ccgccaaccg attgcggcgt tgcagccggt cttccagttc
     3721 tcgaaccgcc gtgacatcct ggatcaggac caggctcccg gcatggcgcc cttcgctgct
     3781 ctgcaaggca cgttgggaga gtgcgacagt ggcgccatgt tgcgtcgtca agggatagac
     3841 atcgccgtcc agctgcgcct ccttgagaca ggcagccaag gagcggccca tgatttcttc
     3901 gcgcgtcagc ccgagcagtt gctcgcccgc ctggttacaa cgtgtgatcg tcccttctgt
     3961 atccatcacg agcaatcccg tcgagaggga ctccacaatt gccgtcaaat gctcccgcaa
     4021 ttcttcattg gcgcgcagat tttggccgag cgcctcgtta cgatcggcca gttccacgtc
     4081 gagctgctcc aacctggacg ccaacgtctg ataggactgt tgcaatgtcg ccgccgcatc
     4141 gtcgaagctc cgaaaggc
//
LOCUS       DATIPF010000182         3320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1537768, whole genome shotgun sequence.
ACCESSION   DATIPF010000182 DATIPF010000000
VERSION     DATIPF010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537768"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1319)
                     /locus_tag="VLL06_04660"
     CDS             complement(54..1319)
                     /locus_tag="VLL06_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HSS30290.1"
                     /translation="MTTTRTPSVAYLQQALREKTREVDVLHRISASISNQLDLEAILK
                     HIVEVVVEVTKADACLLYLLSDNQDELILRASKNPHPKLIGRITIGLGEGITGWVAQE
                     RTRVVIPSNANDDPRFKFFHNLPEDRHQAFVSVPIMAKKEVVGVINVQHKRPRRYQAD
                     EIALLSTIANQVGGAIENARLYDQMQRKALQVETLSQVSETVASNRLIKDVLQLLVTM
                     TAQMMNSKICSIMLLDEASGELRIEATQSLSEQYRRKPNLKVGQGISGRAVKDRRPII
                     VADVTTDRDYIYRDMAKQEGLCSLVSVPMMVREKAMGVINSYTSFPHVFTSEEVKLLQ
                     AIANQAAIAIEHTALVERSFEMQEALAVRKLMERAKGYLMRSRRLTEEEAFKLIQRQS
                     MDLRKSMREIAEAVLLAAELDQRAEKQRG"
     gene            complement(1326..2501)
                     /gene="aroB"
                     /locus_tag="VLL06_04665"
     CDS             complement(1326..2501)
                     /gene="aroB"
                     /locus_tag="VLL06_04665"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HSS30291.1"
                     /translation="MTITGVAEQIVPVMLGERSYDIVLQPGLLTTVGDRLHTLTTSPK
                     IGVVTDRHVAHHYLQGAIRSLRKAGYDPTPIILPPGERTKTLSTIAKILDVLARHKFE
                     RQSLLLALGGGVIGDVTGFAAAIYQRGIPFVQVPTTLVAQVDSSVGGKTGVDHRLGKN
                     LIGAFYQPRAVLIDPHTLRTLPFREWIAGLAEVIKYGIIADQEFFVYLEQEISALLKL
                     KDEPVIHAITRSCEIKARVVAADERESDRRRILNYGHTIGHALESLAGYQGLIHGEAV
                     GIGLVQEADVACYMGLCGRDVVERIRSLVQRAGLSEQVPRASFTSLWGAMQHDKKVIG
                     GRVIGVWPVRIGEVIIRPIEQQVCEAWFRSTHGRGRRVSSGRLSGKHPVRRKVRARR"
     gene            complement(2982..>3320)
                     /locus_tag="VLL06_04670"
     CDS             complement(2982..>3320)
                     /locus_tag="VLL06_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HSS30292.1"
                     /translation="LNVTPQITSRDPKETGKLIMMKVRATRNAVGARSNPAGPSIDRR
                     EANTQVNIRDGETMVIGGVFIDTQNNNVIGIPYLSRVPVLGWLFKQKSESVSKQELLI
                     FLTPTIVKTM"
BASE COUNT          723 a          970 c          846 g          781 t
ORIGIN      
        1 aaaaccgtga cacaatgcgt aacgggttca tctggtttat gcggcgacca gatctatccc
       61 cgctgctttt cagcccgctg gtcgagttct gccgctaaga gcactgcctc ggctatctcg
      121 cgcatcgact tccgcaaatc catactttgc cgctgaatga gtttgaaggc ctcttcttca
      181 gtcagcctcc tcgagcgcat caagtacccc ttagcccgct ccataagctt ccgaaccgcc
      241 agggcctcct gcatctcgaa cgatctctca accagggccg tatgttcgat agcgatagcg
      301 gcctgattcg caatggcctg gagcaacttg acctcttcgc tggtaaacac gtgcgggaaa
      361 gacgtgtagc tattgatcac gcccatcgcc ttctcccgca ccatcatcgg taccgagacc
      421 aatgagcata gcccttcctg cttggccata tcccgataga tataatctcg atctgtggtg
      481 acatcggcca cgattatcgg ccgacgatct ttcaccgcgc ggccactaat gccctgcccc
      541 actttcaggt tcggcttgcg gcggtactgt tcgctgaggc tctgcgtcgc ctcgatgcgc
      601 agttccccgc tggcttcgtc caacaacatg atcgaacaga tcttggagtt catcatttgc
      661 gcggtcattg ttaccaggag ttgaagtaca tctttaatca accgattgga cgccaccgtt
      721 tcggagacct gcgacaacgt ttccacttgc aaggctttgc gttgcatttg atcgtagagc
      781 cgcgcgttct caatcgcgcc accgacttga ttggcaatcg tcgacagtaa cgcaatctca
      841 tccgcctgat agcgccgtgg gcgtttatgt tggacgttga tcacaccgac cacttccttt
      901 ttggccatga tcggtacgga gacaaaggct tgatggcggt cttctggcag gttatgaaaa
      961 aacttgaacc gtggatcgtc gttcgcgttg ctcggaatga cgacgcgcgt tcgttcctga
     1021 gccacccacc cagtgatgcc ctctcccaaa ccgatcgtga tgcggccaat caatttcgga
     1081 tgagggtttt tcgaggcgcg aagaatgagt tcgtcttggt tatccgacaa gagatacagg
     1141 aggcaggcat cggctttcgt gacttcgacc acgacttcga cgatatgttt gagaatggct
     1201 tcgagatcca gttggttgct gatcgaggcg gagatccgat ggagcacatc aacttcgcgt
     1261 gttttctccc gcaatgcctg ttggagatat gcgacagagg gcgtgcgtgt ggtcgtcata
     1321 gagatctacc ttcgtgcacg gacttttcgc cggacaggat gtttgcccga cagacggcca
     1381 ctcgacactc gcctcccacg gccgtgcgtg ctccgaaacc acgcttcgca cacctgctgt
     1441 tcgatcggcc gaatgatcac ctccccaatc cgcaccggcc ataccccgat gacccgccca
     1501 ccgatcacct tcttgtcatg ttgcatagca ccccagaggg acgtaaagga tgcccgtggg
     1561 acctgttcgg ataacccagc ccgctggacc agactcctga tccgttccac aacgtctcgc
     1621 ccacacaggc ccatataaca agccacatcg gcctcttgca ccaatccgat cccaactgct
     1681 tcaccgtgaa tcaacccttg atagcctgcg agtgactcca gtgcatgtcc gatggtatgg
     1741 ccgtaattga gaatccgtcg gcggtcagac tcccgttcat ctgcggcaac cacccgagct
     1801 ttgatttcac aggatcgtgt aatcgcatga ataaccggct cgtccttaag tttcaagagc
     1861 gcagaaattt cctgctccag atagacgaag aattcttgat cggcgataat accgtacttg
     1921 atcacttcgg ctaacccggc aatccattct cggaaaggca gggtccgcaa cgtgtgaggg
     1981 tcgatcaata ccgcacgcgg ctggtagaac gccccgataa gattctttcc cagccgatgg
     2041 tccacgccgg tttttccgcc aacactggaa tcgacttgag ccacaagcgt ggtcggcact
     2101 tgtacaaatg gaattcctcg ttgatagatc gcggcagcaa atccagtgac gtcaccgatg
     2161 actccgccac caagagccag caacagcgac tgacgttcga acttatgcct ggccaacaca
     2221 tcgaggatct tggcaatagt gctgagtgtc tttgttcgct ctcctggcgg caagataatc
     2281 ggagtagggt catatcccgc cttgcgaagc gaacggatgg ccccttgaag ataatggtgt
     2341 gcgacatgcc gatcggtcac caccccgatc ttcggagaag tcgtgagggt gtggagccta
     2401 tcacccactg tcgtaagtaa gcccggctgg agcacgatgt cgtagctccg ttctcccagc
     2461 atcaccggta caatctgttc tgccacaccg gtgatggtca acggtggtct ctcctggccc
     2521 gacttaccaa gcccccatcc caatcctcct ggatagggag gctcggaccc acggtcactt
     2581 cgcctggcgt catggtacgt tgcagcggct gccatcgagg ggggatggta tcacaacggg
     2641 tgggaaacta gaaggttttc agatacaccg ctaagccaac agctcgtgtt tcacggcctg
     2701 tggagtatcg actttagact gtgtaaccac cacatcggtc gttttgatat agacatcacc
     2761 gctgcccgac catgttgtct gtttgattac gatggtattg caagaggata cgccagcccg
     2821 ccgcatgttc ttgcgccgga caggtgcggc atcgatcata tgagacatac gcagcagacg
     2881 agaacgtgcg ctttggagga tagagatgaa atgccaaccc gaacgcctca cctccttgag
     2941 cccaccattg atgaagagtc agaaggtcgg ttcctagctc ttcacattgt ctttacgatg
     3001 gtgggtgtca agaagatgag cagctcttgt ttcgaaactg actcgctctt ctgcttgaag
     3061 agccatccca aaacaggcac gcgagagaga taggggatac caatcacgtt gttgttctgt
     3121 gtatcgataa acaccccgcc gatcaccatg gtctcgccat cacgaatatt gacctgggta
     3181 ttggcttccc gtcggtcaat acttggacca gctggattgc ttcgggcacc gacagcattt
     3241 ctggttgcgc gaaccttcat catgatcaac ttgccagttt ccttcgggtc tcgcgaagta
     3301 atctggggcg tgacattcag
//
LOCUS       DATIPF010000183         1511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_34176, whole genome shotgun sequence.
ACCESSION   DATIPF010000183 DATIPF010000000
VERSION     DATIPF010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34176"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VLL06_04675"
     CDS             complement(<1..291)
                     /locus_tag="VLL06_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30293.1"
                     /translation="MTQPIHGGNIYKAARDRRTPADRIVDFSASINPLGPPAVGLGAV
                     RSALKQIVHYPDPDCWQLRQVLAQQCGVDPDMILVGNGSTELIHLLPRTLAIK"
     gene            complement(288..1250)
                     /gene="cbiB"
                     /locus_tag="VLL06_04680"
     CDS             complement(288..1250)
                     /gene="cbiB"
                     /locus_tag="VLL06_04680"
                     /inference="COORDINATES: protein motif:HMM:NF015164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HSS30294.1"
                     /translation="MTGGELAMACVLDAAIGDPRWVPHPVRWMGSLIAWCDRYVYQLL
                     LSPAKQRVAGVLLAVALPSGAYAAGALLVWFSNSIDPLWGSLVAVLLAWTTLAARDLF
                     NHVVSVQRALQSDSLTEARTAVAKIVGRDTEKMTESDIIRAAVETIAESTADGIIAPL
                     IYLTIGGAPLALAYKAISTLDSMIGHLDERYRWFGWASARLDDLANFLPARITALLLV
                     LAAGVVSRSWSVMQHTWQILLRDGARHPSPNSGHPEAAMAGALGVQLGGINYYAGLPV
                     ERPCLGDPDQSLTRAHIGMAITLMLWTSLAGVLLSIGCLLMVAE"
     gene            complement(1247..>1511)
                     /locus_tag="VLL06_04685"
     CDS             complement(1247..>1511)
                     /locus_tag="VLL06_04685"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HSS30295.1"
                     /translation="GGLAQPFLAYLSWWHVCLATLTAGLVMTVLLGPWPALGCLLIGT
                     GLVRLSTVWFHRMFGGVTGDLLGATNEVAEILFILIVPVVLFR"
BASE COUNT          359 a          461 c          429 g          262 t
ORIGIN      
        1 cttgatcgca agcgtgcggg gcagcagatg gatcaattcc gtcgaaccat tgccgacaag
       61 gatcatgtct gggtcgacac cacactgttg cgccaatact tgtctgagtt gccaacaatc
      121 ggggtccgga tagtggacga tctgcttgag tgccgaacga accgcaccga gccctacagc
      181 gggaggaccc agcggattga tgctggcgct aaaatccacg atccgatcgg cgggagtacg
      241 tcggtcccga gcagccttgt agatattccc gccgtgaatg ggttgcgtca ttccgccacc
      301 atcaggaggc atccaatgct caaaaggaca cccgcgagac ttgtccagag catgagcgtg
      361 atcgccatgc cgatgtgcgc cctcgtcaga gactgatccg gatcgccaag acatggacgc
      421 tcaactggaa ggcccgcata gtaattgatc ccgcccagct gcacaccgag cgcgccggcc
      481 attgcagctt ccggatgccc gctgttcggg cttggatgtc gtgcaccatc acgcagcaag
      541 atctgccagg tatgttgcat cacagaccat gaccgagaga cgaccccagc agccagcacc
      601 agcaagagtg cggtgatgcg ggccggaaga aaattcgcca ggtcatctaa gcgtgccgat
      661 gcccaaccaa accatcggta tcgctcgtcc aaatggccga tcatcgagtc caatgtgctg
      721 atcgccttat aggccagtgc caaaggcgct cctccgatgg tcagatagat cagcggcgca
      781 atgatgccgt cagccgtgct ttccgcaatc gtttccaccg cagctcggat gatatcggat
      841 tcagtcatct tctccgtatc gcgtccgaca atcttggcga cagccgttcg ggcctctgtc
      901 aaagaatcgg attgaagagc ccgttgcacg gatacgacat gattgaagag atcgcgagcc
      961 gcgagtgtgg tccaggccag cagcactgcc acaaggctcc cccataaggg atcgatcgag
     1021 ttgctaaacc agacgagcag tgctcctgcc gcataggccc cgcttggcaa cgcgacagcc
     1081 aacagcacgc cggccacccg ttgcttcgcg ggagaaagaa gaagttgata gacgtaccgg
     1141 tcgcaccagg cgatgagaga tcccatccag cgcaccgggt gtgggaccca gcgcggatca
     1201 ccgattgccg cgtccaagac gcaagccatt gccaattcgc ctccggtcat cggaagagca
     1261 ccacgggaac gatcaagata aagagaattt ctgcgacctc gtttgtcgcc ccaagcaagt
     1321 cacccgtcac gccgccgaac atgcggtgga accagaccgt ggaaagacgg acaagccctg
     1381 tgccaatgag aagacagccc aaggccggcc atggccctaa caacacggtc attacgagtc
     1441 ctgccgtcag ggtggccaga catacgtgcc accaggacaa ataggcgagg aacggttggg
     1501 ccagtcctcc g
//
LOCUS       DATIPF010000184         1984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1360009, whole genome shotgun sequence.
ACCESSION   DATIPF010000184 DATIPF010000000
VERSION     DATIPF010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360009"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="VLL06_04690"
     CDS             <1..965
                     /locus_tag="VLL06_04690"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative inorganic carbon transporter subunit
                     DabA"
                     /protein_id="HSS30296.1"
                     /translation="RIILFCAHGSTSDNNPYESALDCGACGGNEGKPNARVLAMMANN
                     QRMRERLVKVGIDIPPDTHFLAGQMDTTTDEVQLFDLEDVPPTHRADLARLQEDLKKA
                     SALTSQERCGRFPEMQWTMDGQKAKTLVRRRSADWSQVRPEWGLSSNTAFVIGRRELT
                     KGLDLGGRVFLHSYDYREDPNNRLLEVLLTGPQVVGQWINMEHYFSTVDNDVYGSGSK
                     IYHNVVGRLGIMSGPWSDLRLGLAKQAVMNGEVPYHEPMRLLTIVEAPRERIDKLIAR
                     HDVLKHFYHNEWVHLVALEPEEGILYRYRPTSEWAKLSGLAEPE"
     gene            994..1335
                     /locus_tag="VLL06_04695"
     CDS             994..1335
                     /locus_tag="VLL06_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HSS30297.1"
                     /translation="MGPLTLHPMKEIRVIVAGEHRAFVTELLDQVKATGYTIIGNVSG
                     KGHGGMHEAHFMFSEQESLEIVMTVVPEEKVNPILAGLRPLFDRHSGVLFVTDVAVSR
                     KEYFGKKSSEA"
     gene            1709..1873
                     /locus_tag="VLL06_04700"
     CDS             1709..1873
                     /locus_tag="VLL06_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30298.1"
                     /translation="MLESARRAKWLVLLEQLFVLLKGNVVTSYLIIDMTAALMGLVLL
                     GKTLLPRQFA"
BASE COUNT          462 a          545 c          583 g          394 t
ORIGIN      
        1 cacggattat cctgttctgc gctcacggca gtacatctga caacaatccc tacgaatccg
       61 ccctggattg cggcgcctgc ggcggaaatg agggcaagcc gaacgcccgc gtccttgcga
      121 tgatggcgaa caaccaaaga atgagagaac ggttggtcaa ggttgggatt gacattcccc
      181 cagatacgca ctttctcgcc ggccagatgg acacgacaac cgacgaggta caactcttcg
      241 acttggaaga cgtaccgccg acgcaccgtg cagacttagc ccgactccag gaggatctga
      301 agaaggcttc cgcgctcacc agccaagaac gctgcggtcg ttttccagag atgcagtgga
      361 ccatggacgg acaaaaggcc aaaacactcg tcagacgacg gagcgccgac tggagccagg
      421 tcaggcctga gtggggtctc tcaagcaata cggcattcgt gatcggacgg cgcgagctga
      481 caaagggcct ggacctagga ggacgtgttt ttcttcattc ctacgactat cgggaggatc
      541 ccaacaatcg tctgctcgag gtgctgttga ccggtccaca agtcgtaggc caatggatca
      601 acatggaaca ctacttctcc acggttgaca atgatgtgta cggcagcggc agtaaaatct
      661 accacaacgt ggtggggcga ctcggtatca tgtcgggacc ctggagcgat ctccggcttg
      721 gattggccaa gcaagccgtc atgaatggcg aggtgcccta ccacgaaccg atgcggttgc
      781 tgactatcgt cgaagcgccg cgcgaacgga tcgacaagct gatcgcgcgg cacgacgtgc
      841 tgaaacactt ctatcacaac gagtgggtac acctcgtcgc gctggaaccg gaggaaggta
      901 tattgtatcg ttatcggccg acgagcgaat gggctaaact gagcgggcta gcggagccgg
      961 aatgaatcaa ctcatcaagg aaggagatcg actgtgggtc cgttgacatt gcatccgatg
     1021 aaagaaattc gtgtgattgt ggcgggagaa cacagggcgt tcgtgaccga gctgctggat
     1081 caggtcaagg cgaccggcta cacgatcatc ggcaacgtgt cggggaaagg ccatggcggc
     1141 atgcatgaag cgcacttcat gttcagcgag caggaaagcc tcgaaatagt catgacggtc
     1201 gtcccggaag aaaaagtgaa tccgatcctc gccggactca ggccgctgtt cgatcgacac
     1261 tccggggtcc tattcgttac ggacgtggcg gtcagccgga aggagtattt cgggaaaaaa
     1321 agcagcgagg cttgaccaac tcgtgtcgct ggagttgcgc taagtatgtg cgtagaaacg
     1381 tgatgcggtc ggtgtcatag acgttgccgt cgtcgggaat aacatcgttg gatgtgcagt
     1441 cgttttctgt gatggatatt tccttcactt ccagagcgtc tgcacaaact tcggcgccca
     1501 ggacgtggtc tcagggccga ctatgagcca gggagttgaa catctttgaa tgggtctttg
     1561 caaaggggat ctcgcgcagc cgggcggcga caagagatca ctccgttgac cgaaaacccg
     1621 gcgagtggac acgttcagac caaaagcact ttaaaattaa aaggatcttc tggtactgac
     1681 ctacccccct taacccggtc caccccgtat gttagagtcg gcccgccggg caaagtggct
     1741 ggtgctcctg gagcagctgt ttgttctgct caaaggcaac gtggtgacat cctacctcat
     1801 catcgacatg acagccgccc tgatgggtct cgtcttactg gggaagacac tcctgccgag
     1861 acagttcgca taggtcgtcg ctcggctcca tcattgggtc gtcccgccgg ttaagaccac
     1921 cctcacgctc gacacgccac ataccccggc cgcctctcat cccccggcgc agccgctggg
     1981 cttt
//
LOCUS       DATIPF010000185         7571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_831354, whole genome shotgun sequence.
ACCESSION   DATIPF010000185 DATIPF010000000
VERSION     DATIPF010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831354"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VLL06_04705"
     CDS             complement(<1..1078)
                     /locus_tag="VLL06_04705"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HSS30299.1"
                     /translation="MKIVTGSEMQTLDRRTIIEAHIPSAVLMERAGEGIVRHLEQHCG
                     PTRGKTITILCGKGNNGGDGLVVARLLHRRRARVHVMLLTPITDLSRDTAAMYRRFVR
                     VAGRSAIVRFRSADQARPLLASSDILIDALLGTGLTSVVTGTYREAIDLINSVEKPVI
                     AVDIPSGLQADTGAILGRAIRATLTVTCGLPKLGLYIDAGIDRAGIIRVVDIGIPSAY
                     VDAIESRTLLLTRDRAFQSLPERPPSSHKGTFGHAGIIAGSVGKTGAAALAARAALRV
                     GAGLVTVATPSSVNDVLEAKLLEAMTMPLPETKARTLARSGLNRVLAFIQARTAIAVG
                     PGLSTHPETVELVQSLMKHLDRPTV"
     gene            complement(1227..1940)
                     /locus_tag="VLL06_04710"
     CDS             complement(1227..1940)
                     /locus_tag="VLL06_04710"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HSS30300.1"
                     /translation="MPRLGVNIDHVATLRQARGGTSPDPLAAAVLVELAGADGIVVHL
                     REDRRHIQDRDLRLLRELIRTKLDLEMAADETMAKIALSIKPDMVTLVPEQRQELTTE
                     GGLDVAGHRDRIQQVVTLLHDGGIPVSLFIEPDLAQIKASHRVSADFVELHTGRYANA
                     RRSKEADAEVEAITQAAKLSYKLGMGVNAGHGLDYRNVTRLTHIPEIVEYNIGHSIIA
                     RAVLVGLDQAVREMKALLS"
     gene            2225..3421
                     /gene="alaC"
                     /locus_tag="VLL06_04715"
     CDS             2225..3421
                     /gene="alaC"
                     /locus_tag="VLL06_04715"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HSS30301.1"
                     /translation="MGLGDGFYRIKRLPPYVFAQVQSLKLEARQKGEDIIDFGMGNPD
                     QPTPGHIVEKMIEAARKGKNHRYSASRGITKLRHAIAGWYKRNYEVDLDPETETIVTI
                     GSKEGLAHLALALIGPGDVVLTPTPTYPIHMYSFIIAGGEVRGIELRPDSDFFDDLQR
                     VYRQTFPRPKILVINFPHNPTTAVVDLEFFKKIVAFAKEHHVIVIHDLAYADLVFDGY
                     RAPSFLQVDGAKDVGVEFYTLSKAYNMPGWRVGFCCGNREVVGALAKIKSYLDYGIFQ
                     PLQIASVIALNGPQDCVKETVLRYQKRRDVLVSGLNRIGWQVNKPMATMFVWARIPLP
                     YRALGSLEFSKLLLREAKVAVSPGIGFGEGGDEYVRFGLVENEHRTRQAVRGIRKALK
                     LEGSEE"
     gene            3418..4731
                     /locus_tag="VLL06_04720"
     CDS             3418..4731
                     /locus_tag="VLL06_04720"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HSS30302.1"
                     /translation="MISRVGVGIIGFGTVGTGVAKILLENAALISRRVGVPIEIVRIA
                     DLDVTRDRGLRLPPGLLTTDAKQILTDPSIDIVVELIGGCDVAKRIILEAIVAGKQVV
                     TANKALLALHGEEIFAAASRKGVDLGFEASVGGGIPVIQALREGLAANTIQSIYGIIN
                     GTANYILSRMTHEGRSFETVLDEAKQAGYAEADPTFDVAGIDSAHKLAIMVALAYGTP
                     VNFKDVYTEGITHITPLDIAYAKEFGYTIKLLGIAKLVGHEVEARVHPTMLPSSSPIA
                     QVEGVYNAIQLVGDSVGDVVLYGKGAGSMPTGSAVVSDLIAIARNLLKGAVGRVPPAS
                     FQQDQRRPLRMRQMEEISSLYYLRFMVLDRPGVLSQIAGELGRCGISISSVLQQGRRE
                     GQTVPVVIKTHTAMERDVQAALRAINRMAFISEPTTLIRVEGKDE"
     gene            4736..5794
                     /gene="thrC"
                     /locus_tag="VLL06_04725"
     CDS             4736..5794
                     /gene="thrC"
                     /locus_tag="VLL06_04725"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HSS30303.1"
                     /translation="MNRWRGVIEEYRKFLPVTDRTPVVTLGEGNTPLIRATRLAKQIA
                     PDIDLYLKFEGINPTGSFKDRGMTMAISKAVESGANAVICASTGNTSASAAAYGARAG
                     LAVYVLIPAGKIAMGKLSQAMMHQATVIQIEGNFDQALTIVKDLSVIHRIELVNSINP
                     YRIEGQKTAAMEVCDQLGDAPAVHVLPVGNAGNITAYWKGYQEYRAANQSTRLPRMVG
                     FQAAGAAPIVLGRIVETPQTVATAIRIGNPASWDAALVAVKESAGAIDSVSDEEILQA
                     YTAVAATEGIFCEPASAASVAGVMKLSKQGGLRAGETVVCTLTGHGLKDADTAISVST
                     QPKTVRATREDVARLLKV"
     gene            5817..7058
                     /gene="apgM"
                     /locus_tag="VLL06_04730"
     CDS             5817..7058
                     /gene="apgM"
                     /locus_tag="VLL06_04730"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HSS30304.1"
                     /translation="MKYLILHADGIADHPRQELDGRTPLHAASTPYLDRLAQGGELGL
                     LAAAPENVRPGSGLTGTAILGYDPKKYYQGPGPLEAASLGVSIGEHDVVYRCTMVTLR
                     ADAQSRSKGGLNEIKKLGPHVEMDDATAGLISTEEARELIEAINEQLGSEMIQFYPGL
                     GHRHLMVWVNGKSRAVCTDPHVLVGRPIADALPTGDGSDILWKLMDASFQILRDHPLN
                     DERQKAGQKPANCLWLWGQGRAILWPSLSERFKMSGVVISQSDVHRGLGIMAGLGAVD
                     VARLASTDLRAQAAVALDELTKKDFAYVHVELPDEVIFGQDIAAKVKGIEAVDRELIG
                     PLLEGLAKLGSHRIVALCDSGSVHHGQAAEGPGFFAYYDSAAPPSAGTGRKFVEADAR
                     ATSVPSRDATKFVVRLFAKGS"
     gene            7064..>7571
                     /locus_tag="VLL06_04735"
     CDS             7064..>7571
                     /locus_tag="VLL06_04735"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250600.1"
                     /note="catalyzes the formation of 4-phospho-L-aspartate
                     from L-aspartate and ATP, in Bacillus, lysine sensitive;
                     regulated by response to starvation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HSS30305.1"
                     /translation="MALIVQKYGGTSVGTIERIHRVADRVEKAHKDGHRIVVVLSAMS
                     GETDRLLRLAHEVAPLPDDRELDMLLSTGERVTIALLAMALRVRGLDAQSFTGRQVGI
                     MTDSAHTNARITRVSADRIREALGNGVIPVVAGFQGINEQSDVTTLGRGGSDLTAVAL
                     AAALKADRC"
BASE COUNT         1601 a         1999 c         2358 g         1613 t
ORIGIN      
        1 ggacggtcgg tcggtccagg tgcttcatga gggactgcac gagttcgacg gtctcgggat
       61 gtgtcgagag cccaggaccg acggcgatcg ccgtgcgtgc ctgaataaag gctaagacac
      121 gattgagtcc cgagcgagcc agtgtacgcg ccttcgtttc cgggagaggc atcgtcatgg
      181 cttccagtaa ctttgcttcc agcacatcgt tgacgctgct gggagtggcc acggtgacca
      241 ggccggctcc aacacggagt gccgctcggg ccgccaaagc cgccgcgccg gtcttcccca
      301 cggaccctgc aataattcct gcatggccaa aagttccctt atgcgaggag ggagggcgtt
      361 ccggtaaaga ctgaaacgca cggtcgcgtg tcaggagcag ggtgcggctc tcgatcgcat
      421 ccacatatgc ggatggaata ccgatatcga cgacgcggat tatgccggcc cgatcgatcc
      481 ctgcatcgat atagaggccg agtttcggaa ggccacatgt gacggtcaac gtggcgcgga
      541 ttgctcgccc caggatggcc ccagtgtcag cctggaggcc ggatgggata tcaaccgcga
      601 tgactggctt ctcaacactg ttgatgagat cgatggcctc acgataggtt ccggttacga
      661 cagaggtcaa cccagtacca agcagggcat cgataagaat atcgctggag gcgaggaggg
      721 gccgcgcttg gtcggcagat cggaatcgaa cgatcgcaga tcgtcctgca acccgaacga
      781 accgacgata catggccgcg gtatcacgac tcagatctgt gatcggtgtg agaagcatca
      841 catgaacccg agcgcgccgg cgatgcagca gtcgggccac taccaatccg tctcctccgt
      901 tatttccttt tccgcagagg atggtgatgg tcttgcctcg cgtcggtcca cagtgctgct
      961 ccagatgtct gacaattcct tcgccagccc gctccatcaa gacggcactg gggatatgcg
     1021 cttcaatgat ggtgcggcga tcgagtgtct gcatctcgga tccggttacg atcttcatcg
     1081 gtgtcctggc gtgcggaatc ggtcccgcat gaggattcta gtgagcgggg ggaggagaaa
     1141 gttctgttga ttacggcaca tggtcatgac acatgtgaat cgtgttgtcc gtggtggatc
     1201 gaagggtacc gtatcggtgc gacgggttaa ctcagcaatg ccttcatttc cctgacggct
     1261 tggtcgaggc ctaccagaac cgctcgggca atgatactgt ggccaatatt gtattcaacg
     1321 atttcaggaa tgtgcgtcaa gcgcgtcaca ttgcgatagt ccaatccgtg cccggcattg
     1381 acccccatcc caagtttgta ggagagcttg gcggcctgcg taatggcctc gacttctgcg
     1441 tcggcttctt tcgagcgccg ggcattcgca taccggccgg tatggagctc gacaaaatcg
     1501 gctgagaccc tgtgcgaggc tttgatctgg gccaaatccg gttcgatgaa caggctgacg
     1561 ggaatgccgc catcatggag cagggtcaca acctgctgga tacgatctct gtggcctgcc
     1621 acgtcaaggc cgccttcagt cgtaagttcc tgacgctgct ccgggacaag cgtgaccatg
     1681 tccggtttta tggacaaggc aattttggcc atggtctcat cggcggccat ctcgaggtcg
     1741 agcttggtac ggatgagttc ccgcaggagg cgaagatcgc gatcttgaat atggcgccga
     1801 tcttctctga gatggacgac aatcccgtct gcgccggcca attccaccaa tacggcggcg
     1861 gcaagggggt ctgggctggt tccaccacgg gcctgccgta acgtggccac atggtcgatg
     1921 ttcaccccaa gtcgtggcac ggatcctccg ttctggctct gaagagcaag gagaaaccga
     1981 gttgcatgat gcgtacgaaa ggcgttgcag ttgtaacaga aatcgactga gaggggcaag
     2041 gactgggagg gggagttggt atggtgtcgc ctcaggaggt cgaaagggct gaaatgtcga
     2101 accccacaga atcacggcat aatttgactc gacgcgccca ccgacattag aatacgcgac
     2161 ttgttgtggc ggagggtcgt ttctcgatgc tctgcacatg tgaggaggac aagcggggat
     2221 ctcaatgggg ttaggtgacg gattttatcg catcaaacgg ctaccgccgt atgtgtttgc
     2281 ccaggtgcag agcctcaagc tcgaggctcg ccaaaaaggt gaagacatta tcgatttcgg
     2341 aatgggaaat cctgatcaac cgacaccagg ccatatcgtc gagaagatga tcgaagcggc
     2401 gcgaaagggg aaaaatcatc gctactcggc ctcgcgcgga attacgaagc tgcgacatgc
     2461 catcgcagga tggtacaagc ggaattacga agtcgatctg gatccagaaa ccgagaccat
     2521 cgtgacgatc ggctccaagg aagggttagc acacctggcg ctggccttga tcgggcccgg
     2581 tgacgttgtg ttgacgccga cgccaaccta tcctattcat atgtacagtt tcatcattgc
     2641 ggggggagag gtcagaggga tcgagttgcg tcctgacagt gacttcttcg acgatttaca
     2701 gcgggtctat cgccagacct ttccgcgacc caagatcttg gttatcaatt tcccgcataa
     2761 ccccaccact gccgtggtgg acctcgagtt tttcaagaaa atcgtcgcgt ttgccaagga
     2821 acatcacgtg attgtgatcc atgacttggc ctatgccgac ctggtgttcg acggatatcg
     2881 ggcgccgagc tttttacaag ttgatggggc caaggatgtc ggcgtggagt tctataccct
     2941 ttccaaggcc tataacatgc ctggttggcg cgtaggcttt tgttgcggca atcgcgaggt
     3001 tgtcggggcc ttagccaaga tcaagagcta cctggattac ggtatcttcc aacctctgca
     3061 gattgccagc gtcattgccc tcaatgggcc gcaggattgt gtcaaagaga cagtcctgcg
     3121 gtaccagaag cgtcgtgatg tgttggtgag cggactgaat cgcatcgggt ggcaggtgaa
     3181 caagcccatg gccaccatgt ttgtctgggc cagaattccc ctgccctatc gggctctggg
     3241 gtcgttggag ttttcgaagc tcctgcttcg cgaggcgaaa gtagctgtgt ctcctgggat
     3301 cgggttcggt gaaggtgggg acgaatatgt gcggtttggc cttgtcgaaa atgaacatcg
     3361 cacgcgacaa gccgttcgag gcattcgcaa agcactgaag cttgaggggt cggaagaatg
     3421 atttcgcgcg tgggcgtcgg catcattgga ttcggaaccg ttgggactgg ggtcgcgaag
     3481 attctcttgg agaatgcggc cttgatcagt cgccgggtcg gtgtgccgat cgagattgtt
     3541 cggatcgcgg acctcgatgt gacacgcgac cgaggtcttc gacttccgcc cggcctactc
     3601 acgaccgatg cgaaacagat tctgactgat ccatcgatcg acatcgtcgt tgagctgatc
     3661 gggggctgcg atgtcgccaa gcgcatcatc ctcgaagcca ttgtggcagg taaacaagtc
     3721 gtcacggcca ataaggccct gctcgcctta cacggagaag aaatatttgc tgcggcttcc
     3781 cgcaagggcg tcgacctcgg gttcgaagcg agtgtcgggg gggggattcc ggttatacaa
     3841 gcattgaggg aagggctggc agccaacacc attcaatcca tttatgggat catcaatggg
     3901 acggccaatt atattctctc gcgtatgacc catgaaggcc ggagctttga gacggtactc
     3961 gacgaagcga agcaggccgg ctatgctgaa gcggatccga cgttcgacgt ggccggtatc
     4021 gattcggcgc acaaactggc gatcatggtg gcactggcct atggaacacc ggttaatttt
     4081 aaagatgtgt acaccgaagg catcacccac attacgccac tcgatatcgc gtatgccaaa
     4141 gagtttggtt atacgatcaa gctgctgggc attgccaaac tggtgggtca cgaagtggaa
     4201 gctcgcgtgc atcccacgat gctgccctct tcgtctccga ttgcccaagt cgaaggagtc
     4261 tacaacgcca ttcaacttgt cggcgattct gtgggcgatg tggtgctcta tggcaagggc
     4321 gcggggtcta tgccgaccgg gagcgcggtg gtcagcgatc tcatcgccat cgcgcgcaat
     4381 ctgttgaagg gagcggttgg gcgagtccca cccgcttcat tccagcagga ccaacggcgt
     4441 ccgcttcgga tgcgccagat ggaagaaatc agctcgctct attacctccg tttcatggtg
     4501 ttggaccgcc ccggtgtctt gtcgcaaatt gcaggggagt tgggccggtg cggtatcagt
     4561 atctcgtccg ttctccaaca gggccggcgc gaagggcaga cggttccggt cgtgatcaag
     4621 acccataccg ccatggagcg cgatgtccaa gcggcgttgc gagcaatcaa tcgcatggcc
     4681 tttatctccg agcccaccac cttgatcagg gtcgaaggca aggacgagta atgccatgaa
     4741 tcgctggcgt ggtgtgatcg aggaatatcg gaagtttttg ccggtgaccg accgtacccc
     4801 tgttgtgacg cttggtgaag gcaacactcc actcatcaga gcaaccaggc tggccaagca
     4861 gatcgcacca gacatcgatc tctacctcaa attcgaagga atcaatccga caggatcgtt
     4921 caaggatcgc ggcatgacca tggctatttc aaaggccgtt gagtccggtg ccaacgccgt
     4981 gatctgtgcc tccacaggaa atacatccgc ctccgctgcg gcctacggtg cgcgggcagg
     5041 actggcggtc tatgtgttga ttcctgccgg gaagatcgcg atgggtaagt tgtcccaggc
     5101 catgatgcat caagcgaccg tcatccagat cgaaggaaac ttcgatcagg cgttgaccat
     5161 tgtgaaggac ttgtcggtca tccaccgcat tgaattggtc aactcgatca atccctatcg
     5221 gatcgaagga cagaaaactg ccgcgatgga ggtgtgcgat caactcggcg atgccccggc
     5281 tgttcatgtg ttgccggtag ggaatgctgg aaatatcacc gcctattgga aggggtacca
     5341 ggaataccgt gcggcgaatc aatccacgag gctgcctcgc atggtcggat ttcaggcagc
     5401 tggtgccgcg cccattgtgc ttggtcgtat cgtggagact ccacagacgg tggccacggc
     5461 gatccggatc ggtaatccag ccagttggga cgcggcctta gttgccgtga aggagtccgc
     5521 gggagcgatc gattcggtat cggatgaaga aattctgcag gcctatacgg cagtcgccgc
     5581 gacggaggga atcttttgcg agccggcatc tgctgcatcg gtggccggtg tgatgaagtt
     5641 gagcaagcaa ggcggactgc gggcaggtga gaccgtcgtc tgcaccttga ccggccatgg
     5701 attgaaagac gccgataccg ccatcagtgt atcgactcaa ccgaaaaccg ttagagcgac
     5761 gcgggaggat gtcgctcgcc ttttaaaggt gtagatacca tgcgagcagg gcttcgatga
     5821 agtatctcat tctccatgcc gatgggatag cggaccatcc ccggcaggaa ctggacggac
     5881 ggactcctct tcacgcagct tcgaccccgt acctggatcg cctcgcgcaa ggtggggaac
     5941 ttggacttct ggccgcggcg cctgagaacg tgcgaccggg aagtggtctg acggggaccg
     6001 ctatactcgg atacgatccc aagaaatact accaggggcc tggtccgctc gaagcggcca
     6061 gtctcggggt ctcgatcggt gagcatgatg tggtctatcg ttgtacgatg gtcacgttgc
     6121 gtgcggatgc ccaatccagg agtaaaggcg gattgaacga gatcaagaag ctcggaccgc
     6181 acgtcgaaat ggatgatgcg actgcaggcc tcatttcgac agaagaggcc cgcgaattga
     6241 tcgaggcgat caatgaacag ctcgggtctg agatgattca gttctatcca gggctaggcc
     6301 accgtcacct tatggtatgg gtcaacggga agtcgcgagc ggtctgcaca gacccacatg
     6361 tattggttgg tcgaccgatc gccgatgcat tgccgactgg ggacggatcc gatattctgt
     6421 ggaaactcat ggacgcgtcg tttcagatct tgcgcgacca tcccctcaac gatgaacgtc
     6481 agaaggcggg gcagaagccg gccaattgtc tgtggctctg gggccaaggc cgagccattc
     6541 tttggccaag tctctccgag cgattcaaga tgtccggtgt ggtgatctca cagagcgatg
     6601 tccatcgcgg actcggtatc atggccggac tcggagccgt ggacgtcgcg cgattggcca
     6661 gtacggatct tcgcgcccaa gcggcggtcg ctcttgatga actgacgaaa aaggattttg
     6721 cgtacgtgca tgtcgagtta ccggacgagg tcatcttcgg gcaggatatc gcggccaagg
     6781 tgaagggtat cgaagcggtc gatcgcgaac tgatagggcc gctactcgaa ggactggcca
     6841 agttgggctc tcatcgtatc gtcgcactct gcgattcggg cagcgtacat catgggcaag
     6901 ccgcggaggg tccgggattc ttcgcctatt atgacagtgc ggctcccccc tcggccggga
     6961 ctggacggaa gtttgtcgag gcggatgctc gggctacatc tgtgccgtct cgtgatgcca
     7021 cgaagttcgt ggtacggctt ttcgcaaagg gatcctgacg gacgtggcgc tgattgttca
     7081 aaaatatggg gggacgtccg tcggcacgat cgagcggatc caccgtgtgg ccgaccgcgt
     7141 cgagaaggct cataaagatg gccatcgcat tgtggtagtc ctgtcagcga tgagcggaga
     7201 aacggatcga ttgcttcggt tggcgcacga agtcgcgccg cttccggacg atcgcgagct
     7261 cgacatgttg ctgtcgaccg gagagcgtgt gaccatcgcc ttgctggcga tggccctgcg
     7321 ggtgcgggga ctcgatgctc aatcgtttac ggggcgtcaa gttgggatca tgaccgacag
     7381 tgcccatacg aacgcacgga tcacccgggt gagtgccgat cgaattcgtg aagcgctggg
     7441 caacggggtc attccagtgg tcgccggctt tcaagggatc aacgagcagt cggatgttac
     7501 gacattgggg cgcggaggct ccgatctgac agccgtcgcg ctggcagcgg ctttgaaggc
     7561 tgataggtgt a
//
LOCUS       DATIPF010000186         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_34777, whole genome shotgun sequence.
ACCESSION   DATIPF010000186 DATIPF010000000
VERSION     DATIPF010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34777"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VLL06_04740"
     CDS             complement(<1..441)
                     /locus_tag="VLL06_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HSS30306.1"
                     /translation="MQIHATAIVHPKANLADDVEVGPFCLIGEHVTIGSGTHLVSHVA
                     VDGWTEIGARCTLHPFVSIGAPPQHLNYKGEPTKVIIGDDNILREYVTVNRATVQGGG
                     TTTIGSKNFLMAYVHVAHDCHLGSHLILANAASLAGHITIGDHAI"
     gene            complement(523..981)
                     /gene="fabZ"
                     /locus_tag="VLL06_04745"
     CDS             complement(523..981)
                     /gene="fabZ"
                     /locus_tag="VLL06_04745"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HSS30307.1"
                     /translation="MSVMEQAEIQSLLPHRYPFLLVDRVQELDPDRRIVGIKNVTINE
                     PFFQGHFPGRPVMPGVLILEAMAQVGAVLAFKSMGHMTRPAVYLTGIDGAKFRKPVVP
                     GDRLRFEIDVVKKRGPFWKMQGKAFVEAEVVCEAEVTAMVTEEKDVKRDA"
     gene            complement(1320..1856)
                     /locus_tag="VLL06_04750"
     CDS             complement(1320..1856)
                     /locus_tag="VLL06_04750"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HSS30308.1"
                     /translation="MERTSVTGLIAAVALWLAMVSPGLSAETFKVAVMDQQLVVQKSK
                     AGKRAIEELKAYSTTRQKIIEADEQELKELQQALQDEKLTDSAKAEKQGQLQAKAEAY
                     QRRLADFNREIQEKQREMVAEYSRKVQVAAQTVVETNGYVAIIDKGNESAMRIVLYYQ
                     PELDLTNQVVTEFDRQNK"
     gene            complement(1989..2522)
                     /locus_tag="VLL06_04755"
     CDS             complement(1989..2522)
                     /locus_tag="VLL06_04755"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HSS30309.1"
                     /translation="MRKMSCTGGLLMALLAVYGCAAGGAKVEGKVGVVDSARVLNETT
                     SGKKAKDSLSAFSKNRQTLIEMDEKELRRMEEDFGRQASVLSATAKKEREDQFRRRVA
                     EYQQKAGEMNREIQEKQKDALEGFREKVELVVGKVSKRLGLQVIMEKGRGGPTLYSEP
                     GLDITGQVIDEFNREYP"
     gene            complement(2584..>2953)
                     /locus_tag="VLL06_04760"
     CDS             complement(2584..>2953)
                     /locus_tag="VLL06_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HSS30310.1"
                     /translation="PIPLTERFFVGGINTMRGFVFGRAGPVTQFNSLLGAAEQLIFNN
                     DFIFTISSEAKLNGVIFFDYGKGFDDNESISFHLRKSAGLEGRWISPFGPLRAAYGIN
                     LDPRQGERKGVFEFTIGSLF"
BASE COUNT          630 a          905 c          702 g          716 t
ORIGIN      
        1 gatggcatgg tcgccgattg tgatatggcc ggccaaactc gccgcattcg cgagaatgag
       61 atggctgccg agatgacaat cgtgagcgac gtgtacatag gccatcagga agttcttgga
      121 cccgatggtg gttgtcccac caccttgtac cgtcgcccgg ttgacggtga catattcccg
      181 gagaatgttg tcgtcgccga tgatcacctt ggtcggttcg cccttgtaat tcagatgttg
      241 aggcggagca ccgatcgaga caaatggatg cagcgtgcac cgcgcaccga tctccgtcca
      301 gccatcgact gcgacgtgag acaccagatg cgttcccgat ccgatggtca catgctcacc
      361 gatcaggcaa aacggcccga cctctacatc atcggcaaga ttcgccttgg gatgcactat
      421 cgctgttgca tgaatctgca cgctgaacct ccggtgaaat ctacatcgtg aatcgtgatg
      481 cgtgaatggc gagtgcgggg ggcggccaca ggtcttctcg tgtcatgcgt cacgtttcac
      541 gtctttctcc tcggtcacca tggcagtcac ttcggcttca cagacgacct ctgcctcgac
      601 aaaggccttg ccttgcatct tccaaaacgg accccgcttc ttcacgacgt cgatctcgaa
      661 tcgcaaccga tcaccgggca cgaccggctt tcgaaacttc gcgccatcga taccggttag
      721 atacactgcg ggacgcgtca tgtgccccat cgacttaaac gccaacaccg caccgacctg
      781 agccatcgct tcgagaatca ataccccagg catgacggga cgtccaggaa aatgcccttg
      841 aaagaatggt tcattgatcg tgacgttctt gatcccgacg atccgccggt caggatccaa
      901 ctcctgcacg cgatcgacca ggagaaatgg ataccggtga ggcaacaggg actgaatttc
      961 ggcttgttcc atcacagaca tcggcggaac ctcctcgttc gtcaaaccat caactgatga
     1021 cacgtccagg ggtggagaaa tatatgatcc ctccaagctc gctcgttatc tcttcgggga
     1081 agggggctga ttgatcttcc actgcgcgcc acaccccgat cccatatacc ctcatgtcac
     1141 tttcaagggt ggcctggtcg atcctcgatt gcgcgcgtcg aataatcctt ccaagcttgc
     1201 tcgtcatccc tccagggatg ggggctgatt gagcttctac tgcgcgcgtc caacgagggc
     1261 cttctgaggc cgcgcgttgc gcgagcacag aagctcatca ggctccatcc cctcccctgc
     1321 tacttattct gtcgatcgaa ctccgtgacc acctgattgg tcaagtccaa ctcaggctga
     1381 taatagagca caatcctcat ggctgactcg tttcccttgt cgatgatcgc cacatacccg
     1441 ttcgtttcga ctacggtctg cgcagccact tgcacttttc ttgaatattc cgccaccatc
     1501 tcacgctgtt tctcttgaat ctcgcgatta aaatcggcca gacgacgttg ataggcctcc
     1561 gccttggcct ggagctgacc ctgcttctct gccttcgcgc tgtcggtcag tttctcatct
     1621 tgaagcgcct gctgtaactc cttcagttct tgctcgtcgg cctcgatgat cttttgtcta
     1681 gtcgtggagt aggccttgag ctcttcgatt gcccgcttgc cggccttcga cttctgtacg
     1741 accagctgtt ggtccatcac cgcaactttg aaggtctcag ccgacaaccc gggcgatacc
     1801 attgccagcc acaatgccac tgcagcaatc aaacctgtca cgctcgttcg ttccatcgaa
     1861 tcctcctccg ctccttacgg tcttgtctat ctggtctgtt aggtctgtct agtctgtttt
     1921 gtcgcttgat ccggagaacc aaactacacg agacagaccg aacagactag atagaccaga
     1981 cagaccggtt acgggtattc acgattaaat tcatcaatga cctgacccgt gatatcgagt
     2041 cctggctcgc tatataaggt aggaccgcct ctcccctttt ccataatgac ctgcaagcca
     2101 aggcgcttcg acactttccc caccacgagt tccaccttct cacgaaaccc ttcgagcgcg
     2161 tctttttgct tttcttgaat ctcccggttc atttcgcccg ctttctgctg atactccgcc
     2221 acccgacgcc gaaactgatc ttcgcgttcc tttttcgcag tcgcgctcaa cacactggcc
     2281 tgtctcccaa aatcttcttc catgcgacga agctccttct catccatctc gatcagcgtc
     2341 tggcgattct tggagaatgc acttaaggaa tctttggcct tcttgccgct cgtcgtctcg
     2401 ttcaagacac gggccgaatc gacgactccc actttcccct cgactttggc cccgccggca
     2461 gcacagccgt agacagcaag gagcgccatc aacaaccctc ctgtgcaaga catcttccgc
     2521 atgacggcac tcatctggat catcacacag ctacctctca gtcgtggtca acaacgtgct
     2581 tcgttagaac agtgaaccga tggtaaattc gaagacccct ttgcgttctc cctgccgggg
     2641 atctaaatta attccatatg cggcgcggag tgggccaaac ggtgaaatcc atcggccttc
     2701 caatccggct gatttccgca agtgaaacga gatcgattca ttgtcatcga agccttttcc
     2761 ataatcaaag aagatcaccc cgttcagttt ggcttcagag gaaatcgtaa agatgaagtc
     2821 gttattaaag atcaactgct cggctgcacc gagaagggag ttgaattggg tgacaggtcc
     2881 cgcacgcccg aacacgaatc cgcgcatggt attgatgccg ccgacaaaga atcgttccgt
     2941 caatggaatg ggg
//
LOCUS       DATIPF010000187         7801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_301060, whole genome shotgun sequence.
ACCESSION   DATIPF010000187 DATIPF010000000
VERSION     DATIPF010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7801
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301060"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VLL06_04765"
     CDS             complement(<1..253)
                     /locus_tag="VLL06_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HSS30311.1"
                     /translation="MPRPYDQNIPLDTLISVATDAARQGGAVLAECTRIGFRIEHKQI
                     INLVTDADHQAEQRIIDVIRNSFPTHPVLAEERGLTEQPP"
     gene            509..1018
                     /locus_tag="VLL06_04770"
     CDS             509..1018
                     /locus_tag="VLL06_04770"
                     /inference="COORDINATES: protein motif:HMM:NF016924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSS30312.1"
                     /translation="MEELTDILVGLEQRISAYKNRLQELEKKRRRLDDEIATIKKYLE
                     LAETLYRVEADKAKLASLSSQFYSDEKGTRQRPNTDVTDQSREILMGRSKYSGKSVSE
                     AAYEMLREANRSMHAKELVQRLVEGGLQIKGKTPLTSVATSLKRDKRFKKVGPNTFEA
                     LEESLIHAV"
     gene            complement(1246..2181)
                     /gene="lpxC"
                     /locus_tag="VLL06_04775"
     CDS             complement(1246..2181)
                     /gene="lpxC"
                     /locus_tag="VLL06_04775"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HSS30313.1"
                     /translation="MRAQQTLANAVSCAGVGLHSGQPVTLTLKPATPDTGIVFVNRNG
                     KNGASLAASVEHLVPTELCTAISGNGFQVQTIEHVLAALAGLDIDNVYVELTAGEAPV
                     MDGSAAHFVRLIRSAGIIPQSRRQPYLKITRPLEVVDGTRRVRIEPSSTAKITYSIHY
                     NHPMIQTQTYVYEHSAHAFEREIADARTFGFLQEVEAIWARGLGQGGNLDNTIVLSQD
                     GILNESGLRFPNEFVRHKILDLIGDFSLLGVPFIGHLIAERSGHAIHTRLVQQILTHP
                     DSWVLLNADETVAASEPRSAMIAPRSTSLVALQAS"
     gene            complement(2500..3171)
                     /locus_tag="VLL06_04780"
     CDS             complement(2500..3171)
                     /locus_tag="VLL06_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSS30314.1"
                     /translation="MEKIKVLIADDHRVVREGLAAILKMKDDIHIVGEAQDGMEAVEK
                     VKTLVPDVVLMDVSMPRMGGVEATRQIKREFPHIGIVALTMYDEQQYIFDLVRAGATG
                     YLLKDSESSQIVAAIRAIYKGESLIHPSVASKILAEFSLMSQKKGKKPGWVEHDLTER
                     EITVLRLVADGKTNKEIANNLDLSEKTVKNHVRNIFHKLQVYDRTQAAILAIRKGLIE
                     LDPRP"
     gene            complement(3158..4267)
                     /locus_tag="VLL06_04785"
     CDS             complement(3158..4267)
                     /locus_tag="VLL06_04785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HSS30315.1"
                     /translation="MPTTQAMSRKRPRKTGVPSGTHVAIIGAGRGGTALLEVFAADPL
                     VRIVGVAEIKADAPGIALAKRLGIPVTHDFRRLLDLEHVDLIIDVSDNVKVWEMLQDF
                     HRMGVTIIGGASAKFMWELIEARIRATTEIEKALNKYQSLYRLYVKETGVAVTDERTR
                     IACEMHDGLVQNLAGVNFKLDLSLQLIRKDPKASLATLRESKAQLKMAIHEARQAIFN
                     LRPLHYDKMELLPALTNYLTSYETQYHIKTQFRVSGDEQILFPRTKIFLFRIVQEALS
                     NVERHAKATRVTVKLDIDPTLLRVTIGDNGVGFDMDTVLRDPDKWDHFGIRGILERAR
                     LVGGEGKIESKKGHGTTIVVEIPLDKKEATSRGKN"
     gene            complement(4369..4833)
                     /gene="nrdR"
                     /locus_tag="VLL06_04790"
     CDS             complement(4369..4833)
                     /gene="nrdR"
                     /locus_tag="VLL06_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HSS30316.1"
                     /translation="MKCPFCDELEDKVVDSRMAKEGEVIRRRRECLGCKRRYTTYERV
                     DEILPMVVKKDGRRESFDRTKILAGLKKACEKRPISTATIETVTDRIEKRIQEMGETE
                     IESRIVGEELMKELHQLDQVAYVRFASVYREFKDIDQFMDELRTLAQQRRER"
     gene            complement(4864..6126)
                     /gene="glyA"
                     /locus_tag="VLL06_04795"
     CDS             complement(4864..6126)
                     /gene="glyA"
                     /locus_tag="VLL06_04795"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HSS30317.1"
                     /translation="MTNVIGSLDALKATDPDVYDAIVAEEERQRDKLLLIASENFASP
                     AILAAQGSIMTNKYAEGYPGKRYYGGCQHVDTVEDLAIQRCKQIFGAEHVNVQPHSGS
                     QANMAAYLSVLKPGDTILGMDLAQGGHLTHGSKVNFSGILFRVFSYGVERETETINYD
                     AVQKVAEECRPRLLVVGASAYARTFDFPRFQQIAKSVGAYLLVDIAHIAGLIAAGLHP
                     NPVPYADFVTTTTHKTLRGPRGGVVMCKAEHAKGVDKLLFPGIQGGPLMHVIAAKAVA
                     FKEALSPAFTRYQQQVIANAKALAQGLLDRGYKIVSGGTDTHLMLMNLADKGITGKEA
                     DAALGAAGIIVNKNAVPYDEKPPAVASGIRLGTPVVSTRGMREAEMKEIVGLIDRVLQ
                     HRQDPAILEEVRIQAKTLCSRFPIFYTY"
     gene            complement(6144..6617)
                     /gene="rplI"
                     /locus_tag="VLL06_04800"
     CDS             complement(6144..6617)
                     /gene="rplI"
                     /locus_tag="VLL06_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HSS30318.1"
                     /translation="MKVILQETMDGVGHLGDLLDVKDGYARNFLLPRKKAVLADSRSI
                     KAFEHIKRVAGERAKKEKLEIETHAKSISAVSLTVQAQVGKDDKMFGSVTAKDIAERL
                     LEQGFTVDRRKIQLDHPIKELGTFTVPIKLPRDVTATVVIHVVKKQETETAPAEA"
     gene            6780..>7801
                     /locus_tag="VLL06_04805"
     CDS             6780..>7801
                     /locus_tag="VLL06_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30319.1"
                     /translation="MMTIAKHGIFSTDLMTARDLLETWAIRLEAEQTRVSRTAFDQPI
                     THVTGRLVRTIGTLHLYELTLPHGSSIEHDTPLSIILPDNMEPTEGIVLGGQGNVVLV
                     QTFDAIGQTCPDATVIPDRAGFLATSAKRLRDMLTQPDSYRLGPADRLASLLEATTGA
                     EELSGIGAGSSILTTVWSDELSVRRQRLGVLAIDLIRANKRILVVCPDHQDADAFVGT
                     IARAMKAVGLTYKTWVSRYEMALAHQVEGIGIQELGFEAQMHQFYAKSRADKAALRCK
                     YERFRELTPVLAYKGQKQKDLDEVRLLEWRLLTQVSDLQAKIKDVNATLAEYENLPLF
                     RRLSLQA"
BASE COUNT         1790 a         2119 c         2102 g         1790 t
ORIGIN      
        1 atggaggctg ttcggttagc ccacgttcct cggcaaggac tggatgagtc gggaaactgt
       61 tgcggatgac gtcgatgatc cgttgctccg cctggtggtc ggcgtcggtg acaaggttaa
      121 tgatctgctt atgttcaatg cgaaaaccga tgcgtgtgca ttccgctaga acagctccac
      181 cttggcgggc tgcgtcagtg gcgactgaga tgagtgtgtc gagaggaata ttctgatcgt
      241 agggtcgcgg catgagacgg catcatagca cgaggctgtg gtgtactgat gtggacgaag
      301 gagacatcaa attggaagga atcattctat gaggccaaga tttgactgat ttcttatcac
      361 tcttattgag agagcaagtg acgtcatgag caagcacgga cacgttttaa agcgaacatc
      421 ccatgcaagc catgttcgta actttatcta ttgacttgct atgcaagcaa tgctataaag
      481 caagcatgct tcacctgggg gcccgtctgt ggaagaactc accgatatat tggttggtct
      541 tgaacagcga atcagcgcct acaaaaaccg gctccaagag ctcgaaaaga aacgtcgtcg
      601 gttggatgac gaaattgcca cgatcaagaa atacttggag ttggcagaaa cgctctatcg
      661 cgttgaagcc gataaggcca agctcgccag tctctcgagt caattctatt ccgatgaaaa
      721 aggtactcgt caacgaccaa atacggatgt caccgaccag tcgagggaaa tcctcatggg
      781 gcgtagcaag tattcaggga aaagtgtgtc ggaagccgcg tacgaaatgc ttcgcgaagc
      841 caatcgttcg atgcatgcaa aagaacttgt gcagcgattg gttgaggggg gactccagat
      901 caaagggaaa acaccactga catctgtggc cacatcgtta aagcgggata agcggttcaa
      961 gaaagtaggt ccgaatacgt tcgaggcatt ggaagaatcc ttgatccatg cagtgtagtg
     1021 tgtctgggct agtgagacct caaaatacac gaaggggggt gagagtcttg gcgacgaaga
     1081 aagcagcaaa gaagaagaag aagtagtccc cgccttgatt ctagcgtcgg gagcgaggcc
     1141 acttgctccc gacgttaaat cacattccag taactctcgc gtcacaattc aactcacctg
     1201 ttgcggcaag accacgcgca gatttcacct tcaagaggat gagggttagg aggcttggag
     1261 ggccaccagc gacgtggatc gcggtgcgat catggcagaa cgaggctcag acgcagcgac
     1321 tgtttcgtcg gcgttgagca acacccagct atcggggtgg gtgagaatct gctgaacgag
     1381 gcgagtgtgt atcgcatgcc ccgaacgttc ggcgatcaag tgcccgataa agggtacacc
     1441 gagcaacgaa aagtccccga tcaggtccag aatcttgtgt cgcacaaatt cattgggaaa
     1501 ccgaagacct gattcattga gaatgccgtc ttgagagagc acgatcgtat tgtccaggtt
     1561 gccaccctgg ccgagcccac gagcccaaat ggcttctact tcttgcaaga acccaaatgt
     1621 cctggcatcc gcgatttcgc gctcaaatgc atgggctgag tgctcataga cgtaggtctg
     1681 agtctggatc atcggatggt tgtagtggat ggaataggtg atcttggcag tcgaggatgg
     1741 ttcgatgcga acccgacgag ttccatccac cacctcgaga ggccgtgtga ttttcagata
     1801 gggttgccgc cggctctggg gaataattcc tgctgaccga atcaagcgaa caaagtgtgc
     1861 cgcgctaccg tccatgacgg gggcctcacc ggccgtaagc tccacataga cgttatcgat
     1921 atcgagcccg gccaatgcgg ccaggacatg ttcgattgtc tgtacttgaa acccattgcc
     1981 gctgattgcg gtacagagtt ctgtcggcac caaatgttcg acagatgccg cgagggaggc
     2041 tccgtttttc ccgtttcgat tgacgaagac gatgcccgta tcgggtgtgg caggcttaag
     2101 cgtgagggtg actggttgtc ccgagtggag cccaacccct gcacaactca ctgcattcgc
     2161 taatgtttgt tgagctctca cacaaacccc tcaataaatt acgcagagag taccagcaac
     2221 tcgtgtgcca tccagaagtt tttctaaaaa atatcataat ttattgataa taaaagataa
     2281 tatatagagt tcagtagcct gaagtcatgt tgcaaaaaca taacacttgt gacttttcaa
     2341 agaattatcg aatagaaatt cacgctcctg gtgatgaggg gccaatccag caggacaggg
     2401 agtcgatcac cgcagttggc cgcacgggtt gacggaatgg ctcacaatct gccagtggct
     2461 gtctcgggcg agaaagtatc cacctaatga acacaagacc tatggccggg gatccaactc
     2521 aatgagtcct tttcgaatag cgagaatagc agcctgtgta cggtcgtaga cttgcagctt
     2581 atggaaaatg ttgcgcacat gattcttcac ggtcttttcg ctcaagtcca agttattggc
     2641 tatttccttg ttcgtcttgc cgtccgcgac caagcgtagg accgtgatct cccgttccgt
     2701 cagatcgtgt tcgacccatc caggcttttt ccctttcttc tgggacatca aggaaaactc
     2761 agccaagatc ttgcttgcca cggatggatg gatgagtgac tcccccttgt agatcgcacg
     2821 aatcgcagcg acgatctgtg aggactctga gtcctttaag agatagccgg ttgcgccggc
     2881 gcgcaccaaa tcgaagatgt attgttgctc gtcatacatc gtcagggcta cgattccgat
     2941 gtgagggaac tcccgcttga tttgcctggt tgcttcgacc ccacccatcc ggggcatact
     3001 cacatccatc agcacgacat cagggaccaa cgttttcact ttctcgacgg cttccatacc
     3061 atcctgtgcc tcgcccacga tatgaatgtc gtctttcatc ttgaggattg cggccaatcc
     3121 ttcgcggacg acgcgatggt cgtccgcgat gagcacctta attttttcca cgggatgtcg
     3181 cctccttttt atccaaaggg atctcgacaa cgatggtcgt gccatgccct ttcttagact
     3241 cgatcttgcc ttcgccgccc acgagccgag cccgctccaa aattccgcga atcccgaagt
     3301 ggtcccattt gtcgggatca cgtagcaccg tgtccatgtc gaatcccaca ccattgtccc
     3361 cgatggtgac gcggaggagt gtggggtcga tgtccagctt cacggtgacc cgagtcgctt
     3421 tcgcgtgtcg ctccacgtta ctgagagctt cttgcacaat gcgaaagaga aatatcttgg
     3481 tccgcgggaa caatatctgc tcatccccgg atacgcggaa ctgcgtcttg atatgatact
     3541 gggtctcgta cgaggtaaga tagttcgtga gggctgggag tagctccatc ttgtcatagt
     3601 ggagcggacg gagattgaat atggcttgcc gcgcctcatg gatagccatt ttcaactgag
     3661 ctttactttc gcgcaaggtg gccaggctgg ctttcggatc ttttcgaatc agttgcagac
     3721 tcagatccag tttgaaattc accccggcca aattctgcac gaggccgtcg tgcatttcac
     3781 acgcaatgcg tgtccgttca tccgtcacgg ccacacccgt ctccttaaca tacaggcgat
     3841 agagcgattg gtatttgttc agggctttct cgatctctgt cgtggcgcgg atacgggcct
     3901 cgatgagctc ccacatgaat ttggcacttg ccccgccgat gatggtgacg cccatccggt
     3961 ggaagtcttg aagcatctcc cagaccttga cattgtccga cacgtcgatg atgagatcca
     4021 cgtgctcgag gtccaagagc cgccgaaaat cgtgggtcac gggaatgccg agacgctttg
     4081 ccagcgcaat gcccggggcg tccgccttga tttcagccac accgacgatc cggaccagcg
     4141 gatcagccgc gaagacctcc aacagcgccg tgccaccgcg gcctgcgccg atgatcgcca
     4201 catgcgtacc gctgggtact ccagtcttgc gtggccgttt gcgagacata gcttgggtcg
     4261 tcggcatctt gacgcctact cgttaagaca tcgcatcatg ttgtagaccg acgagcggcg
     4321 gaaggacatg gagcgaacga tgagaatacg ggagcgaccc tgtggccctc acctctcgcg
     4381 gcgctgttga gccaatgttc tcagctcgtc catgaactgg tcaatgtctt tgaactcgcg
     4441 gtagacagac gcaaaacgaa cataggcgac ctgatccaat tgatgaagct ctttcatcaa
     4501 ctcttcgccc acgatccgac tctcgatttc agtttcaccc atttcctgga tgcgtttctc
     4561 gatgcgatcc gtcacagttt cgatggtagc agtactgatc ggtcgctttt cgcaggcttt
     4621 cttgaggccg gcgagaattt ttgtacggtc gaacgactcc cttcggccat cctttttcac
     4681 caccatggga aggatttcat ccacgcgttc gtaggtggta tagcgacgct tgcagccgag
     4741 acattcccgt cgacggcgaa tcacctcgcc ttccttcgcc atgcgagagt ctacgacctt
     4801 gtcttcgagt tcatcgcaga acggacattt cacaggcggt gacccagtcc cgtctgacgc
     4861 ggcttagtag gtataaaaga tcggaaaacg actacagagt gttttggcct gaatacggac
     4921 ttcctcaagt atcgcgggat cttgccgatg ctgtagcacc cggtcgatta agcccacaat
     4981 ctctttcatc tcggcttccc gcatcccacg ggtggagaca acgggagttc ccagccgaat
     5041 accactggcg acagccggag gcttctcatc atagggcacg gcattcttgt tcacgataat
     5101 gccagcagca cccaaggccg catccgcttc tttgcctgta atgcccttgt cggccagatt
     5161 catcagcatc aggtgcgtat ccgtgccgcc cgatacaatc ttgtacccac ggtcaagaag
     5221 cccttgagcc aacgccttcg cattggcaat aacctgctgt tggtatctcg taaaagccgg
     5281 cgagagggct tccttgaacg cgacagcctt tgccgcgatc acgtgcatca agggcccacc
     5341 ttggattccg gggaagagca atttgtcgac ccctttggca tgctcggcct tgcacatcac
     5401 gacgccaccc cggggtcccc gcagagtttt atgggtcgtc gtagtgacga agtcggcata
     5461 gggaacggga ttcggatgga gccccgcagc aatcagcccg gcaatgtggg caatatcgac
     5521 cagcaggtaa gcaccgaccg acttagcgat ctgttggaat cgtggaaaat cgaaggtgcg
     5581 cgcatacgca ctggcgccca ccacgagcag gcgtggtcgg cattcttctg ccaccttctg
     5641 caccgcgtca taattgatcg tttcggtctc gcgttccacg ccgtaagaaa acacacggaa
     5701 caggatccct gaaaaattga ccttgctgcc gtgagtgagg tggcctcctt gggcgaggtc
     5761 catgcccaga atggtgtcgc ctggcttcag cacggacaga taggctgcca tattggcttg
     5821 tgaaccagag tgcggctgaa cgttcacatg ctccgcgccg aaaatctgct tacaccgctg
     5881 gatggcaaga tcctctacgg tatccacatg ctgacagcca ccatagtacc tcttccccgg
     5941 gtacccttca gcatacttat tggtcatgat ggagccctga gctgccagaa tggcgggact
     6001 ggcgaagttc tccgaagcga tcagaagcag cttgtcacgc tgccgctctt cctcggcgac
     6061 gatcgcgtca tagacatccg gatctgtagc tttgagtgcg tctaaagaac cgatcacatt
     6121 ggtcatgaca gctcgttccc tcgctaggcc tcagctggag ccgtttcggt ttcctgcttc
     6181 ttcacgacat ggatcaccac ggttgcagtg acatcccgtg gaagcttgat cggtacggta
     6241 aaggtgccga gttccttgat cggatggtcc agctggattt tgcgtcgatc caccgtaaat
     6301 ccctgttcca gcaatctctc tgcaatgtct ttggccgtaa ccgacccgaa catcttgtcg
     6361 tccttgccga cctgagcctg taccgtcagg gacacggctg aaatgctctt cgcgtgggtt
     6421 tcgatctcca gcttttcctt cttcgcccgt tcgccggcga cgcgcttgat atgctcaaag
     6481 gccttgatgc tgcgactgtc ggccagcact gctttcttgc gtgggagcaa aaagttccgc
     6541 gcgtacccat ctttcacatc gagtagatcg ccaagatgtc cgaccccatc catcgtttct
     6601 tgtagaataa ccttcatgcc cctaactcct tctgttggaa aggaaaagag gatactgggg
     6661 gaccgacaaa aagtcaattc gcaatgtgca gaggatgttc ctcaggccag gtcgcgagtg
     6721 cgatcatgaa cggaaaagta tccccgtaac aggctggaca ggtgtaagag cccgaccgta
     6781 tgatgacgat cgccaaacat ggaatatttt ctacggatct catgaccgca cgagaccttc
     6841 tcgaaacatg ggcaatccgg ctggaagccg aacagacacg ggttagtagg acggccttcg
     6901 accaaccaat tactcatgta acaggccgac ttgtccgcac catcggcacc cttcatctgt
     6961 acgagctgac acttccgcac ggctcatcta tcgagcatga cacccccctc tcgattattc
     7021 ttccggacaa catggaacca accgaaggca tcgtacttgg cgggcaaggc aatgtagtcc
     7081 tcgtgcagac gtttgatgcc atcggtcaga cctgtccaga cgcgaccgtg attcccgata
     7141 gagccgggtt tctggcaacg tcggccaagc gactacgcga catgctcacg cagccagact
     7201 cctatcgtct cggtccggca gaccgtttgg cttccctcct cgaggcgacg acgggagcgg
     7261 aggagctttc cgggatcggc gcaggatcgt cgatcctcac gactgtctgg tcagacgaac
     7321 tatcggtgcg caggcagcgg ttgggcgtac tggcgattga tttgattcgc gcgaataagc
     7381 gcatcctcgt ggtctgtcct gatcaccagg atgcagacgc gttcgtcgga accatcgcac
     7441 gagccatgaa agctgtgggg ctcacgtata agacatgggt gagtcgatat gaaatggcat
     7501 tggcccatca ggtcgagggt atcgggattc aggagctggg attcgaagca cagatgcacc
     7561 agttctatgc caagtcgcgc gcagacaaag cggcactccg gtgcaagtac gagcggtttc
     7621 gagagttgac gcctgtgttg gcatacaagg ggcagaagca aaaggatctc gatgaagtgc
     7681 gcctccttga atggcgattg ctgacccagg tcagcgactt acaggcgaag atcaaagacg
     7741 tgaacgccac attagctgaa tatgagaacc tcccgctctt ccgacggctc agcctccagg
     7801 c
//
LOCUS       DATIPF010000188         3616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_301573, whole genome shotgun sequence.
ACCESSION   DATIPF010000188 DATIPF010000000
VERSION     DATIPF010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301573"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VLL06_04810"
     CDS             complement(<1..500)
                     /locus_tag="VLL06_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30320.1"
                     /translation="MKRAPYFLSALLAVMLMQAGCSSSVLQLREQLDLHHGEFRQKLA
                     DKPELETKFFACIGQAHDESLGESRDTRTSSEQLPLQTHQGRVMSIRPLAAAIDTIRR
                     GHQEKGASLSILADILEDVTDDVTARIDLTKLARVVETAGQWHGHFGLDEDKLEQDSS
                     RFARML"
     gene            complement(709..1239)
                     /locus_tag="VLL06_04815"
     CDS             complement(709..1239)
                     /locus_tag="VLL06_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HSS30321.1"
                     /translation="MTHTHSAGSRIEHLFETVVFASRWIQAPLYGGLIVAELLYAYKF
                     LVELWEMISHINQQQETVFMLGVLGLIDVTMVANLLTMVIIGGYATFVSKLDLEGHPD
                     RPDWLTHIDPGTIKIKLAASLIGISSIHLLKSFVDIEHEDPEHIKWKIFIHMTFLGSA
                     ILLAWTDKIMQKERKH"
     gene            complement(1369..1779)
                     /locus_tag="VLL06_04820"
     CDS             complement(1369..1779)
                     /locus_tag="VLL06_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS30322.1"
                     /translation="MTIVLADADAHFRQRLKKRLEKITGVNVVGESSDLEETTAMILN
                     RKPDIAILNSSLRDGCGAEVLRHIKRLMVPPTIIVVTDDLSHGNKNDCSLAGADFFFD
                     KGTEDHKMMHTVRLLCAPHSQLAELDSPIATRND"
     gene            complement(1989..2636)
                     /locus_tag="VLL06_04825"
     CDS             complement(1989..2636)
                     /locus_tag="VLL06_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSS30323.1"
                     /translation="MIETQLVKILVIDDHEVVRRGVKQILEENFLYVEVGEADTAQKG
                     IAAVRQEPWDLAIVDISLPDQNGLELLCELHSTAPQLPLVVLSLHPEEQYAIRAFRAG
                     VMAYLTKHTAPEELAKAVKQVLNGRMYVTTSLAEQMAGSLSKNPTGPEHHTLSEREFE
                     VLVLLARGQSVKHIAQSLTLSIKTVSTYRARLLDKLQLSTTAELIRYALDHHLVE"
     gene            complement(2633..>3616)
                     /locus_tag="VLL06_04830"
     CDS             complement(2633..>3616)
                     /locus_tag="VLL06_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HSS30324.1"
                     /translation="RAAEVASGWKANEALGRNYMEFCLQVEVRESFLTKLAQVIEGMD
                     VRGFESPLLTRMGLQTLFSWNISRVLGTHGELTGLMAIGTAVLSATQAEEELRLAHAR
                     IRSEARRAQSAAEEERRRIARELHDEFGQALTGLKFDLAWFGMKLRQSSAPSGCRDLL
                     NKVQAMSGSVDALLESVRATAASLRPAVLDDLGLVPALECLATTFRLRTDSSCTLDVA
                     TALSSIALPPETSTALFRIAQELLTNVMRHAGASQIRIQLYEGDGSVTLEVTDNGKGI
                     DRERMITPASFGLRGIQERVSLLGGHFHIVGTSGVGTTACISVPVAECFVR"
BASE COUNT          800 a          987 c         1020 g          809 t
ORIGIN      
        1 aacaacatcc gagcgaagcg agaggaatcc tgttcgagtt tatcctcatc cagaccgaag
       61 tgcccatgcc attgcccggc cgtttctacg acccgtgcga gtttggtcag atcgatgcgc
      121 gccgtcacgt catcggtcac gtcttcgagg atgtcggcga gaatggagag agacgcgccc
      181 ttctcctgat gcccgcggcg aatcgtgtcg atggcggctg ccagaggacg gatgctcatc
      241 actcgtccct gatgggtttg cagggggagc tgctcggacg aggtacgagt atcacggctc
      301 tcgcccaatg attcgtcgtg ggcttgcccg atacaagcga agaacttcgt ttcgagctct
      361 ggcttgtcgg ccaacttctg gcggaactct ccgtgatgga gatccagttg ctcgcgaagt
      421 tgcaggaccg aagaactgca gcctgcttgc atgagcatga cggccagcag ggccgacagg
      481 aaatatggtg ctcgcttcat tgcgacctct cggtagtcgt ggctgttgag cctagattat
      541 tgaaccgacc cgttgggata cgcaacagcc atgccgacag aagacagatt cggcagagaa
      601 tgaatcttgg atttatctga aacattcgaa cgccccggtg tgtggcattc gtacgttggg
      661 tattgaatca gatacttccg tatcgaatgc gataccgtcg catttgtgtc agtgcttgcg
      721 ttctttctgc ataatcttat cggtccacgc gaggaggatg gcagacccga ggaacgtcat
      781 gtgaataaag atcttccact tgatgtgttc tgggtcttca tgctcgatgt cgacgaacga
      841 tttgagaaga tgaatgctgg agataccgat cagcgaggcg gcaagtttaa tcttaatggt
      901 gccggggtca atgtgggtca gccagtccgg tcgatcggga tggccctcca aatccaattt
      961 gctcacgaag gtggcgtagc cgccgataat caccatcgtg agcagattcg ccaccatggt
     1021 gacgtcgatc agtcccagga cccccagcat gaagacggtc tcttgttgct gattgatgtg
     1081 gctgatcatc tcccacagtt cgacgagaaa cttgtacgcg tagagcagct cggccacgat
     1141 gagaccgcca tagagggggg cttgaatcca gcgactcgcg aacacgacgg tttcaaacag
     1201 atgctcaata cggctaccgg cagagtgggt gtgtgtcatt gcggtgggtt ctgaaggatc
     1261 ggaatggctc acggctcggt atcctagtcg gcgaaggcgc gacctgtcaa ttcgaataag
     1321 accactaaat tcatgaggga ggtgacggtg atcgcgaagg agcgtggatc aatcattgcg
     1381 cgtggcaatg ggagagtcca attctgcaag ctggctatga ggtgcacaaa gaaggcgaac
     1441 ggtatgcatc atcttgtgat cctccgtgcc cttgtcaaaa aagaaatcgg ccccagcgag
     1501 agagcagtca ttcttattgc catgcgagag gtcgtccgtg acgacgataa tggtcggcgg
     1561 gaccatcaat cgcttgatgt gtcggagaac ttcggcgccg cagccatccc ggagcgagga
     1621 gttcaggatt gcgatatccg gtttgcggtt cagaatcatc gcggtagttt cttccaagtc
     1681 cgacgattct cccacgacgt tcacgccggt aattttctca aggcgcttct tgagcctctg
     1741 gcgaaagtgg gcatctgcgt cagcgaggac gatggtcatc agatggtttc ccccagggtt
     1801 gacaggcttt cgagtcgtac atagtcttta gaatcgatca aaacaaaaaa caatcagccg
     1861 aacagacacg cactgtaagc ggagtccgac gccggactca tccccacata gagacaattg
     1921 gttcccaccg acgtctattc tttcagtgcc ctacagacta ttcatgcatg agtgactcat
     1981 catggcgctt attcaaccaa atgatggtcc agcgcataac ggatcaattc tgcagtcgtc
     2041 gagagttgca gcttgtccaa aagacgggct cgataggtac tgacggtctt gatgctgagc
     2101 gtgagagact gtgcaatgtg tttgacggat tggcccctgg ctaacaagac aaggacttcg
     2161 aattctcgct cagacaatgt gtgatgttcc gggccagtgg gattcttgct gaggctgccg
     2221 gccatctgct ctgcgaggga ggtggtcaca tacatccggc cgttcaggac ctgcttgacg
     2281 gctttggcca gctcttctgg cgcagtgtgt ttggtgagat aggccatcac gccggcacgg
     2341 aatgcgcgaa tggcatattg ctcctcgggg tgcaggctca acacgacgag agggagttgg
     2401 ggcgctgtgc tgtgtaactc acacaaaagt tccaacccat tctgatcggg aagactgata
     2461 tccacgatgg ccagatccca aggctcctgc cgcactgcgg caatcccctt ctgtgcggta
     2521 tcggcttccc cgacctcgac gtacaggaaa ttctcttcaa gaatctgttt cacgcctcga
     2581 cgcacaacct cgtgatcgtc gatgacaaga attttgacca actgtgtttc aatcatcgga
     2641 cgaagcactc tgcaaccggc accgagatgc aggccgtggt cccgaccccg gacgttccaa
     2701 cgatatgaaa gtggccgccc agaagggaga ctcgttcctg aattcctcga agcccaaagg
     2761 aagcaggtgt gatcatcctc tcgcggtcga tacccttccc gttgtcggtc acttctaacg
     2821 tcacgctgcc gtcgccctcg tacagctgta tgcgtatctg cgaagcgcca gcgtgacgca
     2881 tcacattcgt cagcaattcc tgcgcgattc ggaataatgc ggtagatgtt tccgggggca
     2941 gtgcgatgga tgacaacgcc gtcgccacat ctaacgtgca gctcgagtcc gtgcgaagcc
     3001 ggaacgtcgt cgcgagacat tccaacgccg ggaccagtcc gagatcgtcc agcactgcag
     3061 gacgcagaga tgccgcggtc gcccggaccg attccaggag tgcgtcgact gaacccgaca
     3121 ttgcctgaac cttattcagc agatccctgc atccgcttgg agcagatgat tgcctcagct
     3181 tcatgccaaa ccatgccaaa tcgaacttta agccggtcaa ggcctgtccg aattcgtcgt
     3241 gtaattcccg tgcaattctg cgacgttcct cttccgcggc gctctgtgct cgacgagcct
     3301 cgctacgtat tcgagcatgg gccagccgga gttcctcctc tgcctgagta gccgagagga
     3361 ccgcggtacc gatggccatg agtcccgtca gctccccatg agtccccagg acccgggaga
     3421 tattccagga gaagagcgtc tgtagtccca tacgtgtcag gaggggactt tcgaaccccc
     3481 tcacgtccat tccttcaatg acctgcgcga gtttggtcag gaacgactca cggacttcca
     3541 cctggagaca gaactccata tagttgcgac cgagtgcttc gttggccttc cagccagatg
     3601 cgacttcggc cgcgcg
//
LOCUS       DATIPF010000189         1756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_741168, whole genome shotgun sequence.
ACCESSION   DATIPF010000189 DATIPF010000000
VERSION     DATIPF010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741168"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1412
                     /locus_tag="VLL06_04835"
     CDS             <1..1412
                     /locus_tag="VLL06_04835"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HSS30325.1"
                     /translation="LDKPVEAMLTLIKTMAHDQPVTLRLQNDFLFLGDSHLKVNAQQM
                     AVATSIIDALNELKIGGITFSLTVESKDLREFAALFVSLDPDNKSIDDIRKEITARGI
                     VGIEIEEQRQLQIRTGVETAGGTGSGSSKGTGSGPSGNTGSEGTGSADQKIQRKVNAK
                     NGYAKVAGSVGTLTQSSREGGSISFKQAKRAIQNIIDLMVDDETAVLGLTTLRCHDQY
                     THNHSVNVSLLSMALGNRVGYPKVDLADLGLASLFHDVGKCAISLDILNKPGEFTNEE
                     WEIMRTHPTEGVLALIKLRGLSEVPARMAAASFEHHMNYDFSGYPKLAAPWTQSLSSR
                     IVTIADCYDAMTSSRVYRREPMSPSKALNLMLSKSGKSFDAVLLKLFVNCVGIIPIGS
                     LVMLETSELAVVLKPATDKAQAERPFVKVITDPQGNSIDHGREVDLTEKNETGDFRHE
                     ILRLIDNTEYKFDTSRYFV"
     gene            1518..>1756
                     /locus_tag="VLL06_04840"
     CDS             1518..>1756
                     /locus_tag="VLL06_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HSS30326.1"
                     /translation="MHTLGINLGRMTRPLVAAGMLLLASLPAAQAAEPFKMGIVDPQA
                     VLEKSKAGKKALDTLKEYVTARQKLLAADEEELRRT"
BASE COUNT          444 a          490 c          461 g          361 t
ORIGIN      
        1 cactggataa accggtcgag gccatgctga ccttgatcaa gaccatggca catgaccagc
       61 ccgtcacgct ccgcctgcag aacgatttct tgtttctcgg ggatagtcac ttgaaagtga
      121 acgcgcagca gatggccgtg gccacaagca tcatcgacgc tttgaacgaa ttgaaaattg
      181 gcgggataac cttttcattg actgtggagt cgaaagacct ccgtgagttt gccgcactct
      241 ttgtcagcct ggatcctgac aacaaaagca tcgacgatat tcggaaagaa atcacggcac
      301 ggggaatcgt cggcattgag atagaagaac agcggcaact gcaaatccga accggagtcg
      361 aaacggcggg gggcacgggc tccggatcgt cgaaaggcac aggttccgga ccatcgggga
      421 acacaggttc agaaggcaca ggaagcgccg atcaaaaaat acaacggaag gtgaacgcca
      481 aaaatggcta tgccaaagtg gctggatcag tcggaactct gacgcaatcc tcccgtgagg
      541 gaggatcgat cagtttcaaa caggcgaagc gggccatcca aaacatcatc gacctcatgg
      601 tcgatgacga gaccgcagtc ctcggcctta cgactcttcg ttgccacgat cagtacacac
      661 ataaccattc ggtcaatgtc tcgttgctgt ccatggccct cggcaatcga gtcggctatc
      721 cgaaggtaga ccttgccgac ctcgggttgg cttcactatt tcacgatgtt gggaaatgtg
      781 cgatttctct ggatattctc aataagcctg gcgagttcac aaatgaagag tgggaaatca
      841 tgcggactca cccgactgaa ggggtgctgg ccctcattaa gctgcgcggt ctcagcgagg
      901 tgccggcacg catggccgcc gcttcgtttg agcaccatat gaactacgac ttttccggct
      961 atcccaaact ggctgctcca tggacacaga gtctctccag tcgaatcgtg acgattgccg
     1021 attgttatga tgccatgacc tcctctcgtg tctaccgccg cgaaccaatg tccccatcga
     1081 aagcgttgaa tctgatgctc tcaaaatcag ggaaatcgtt tgatgcggtg ctgctcaagc
     1141 tgttcgtaaa ctgcgtcggc atcatcccta tcggcagcct ggtcatgctg gagacaagcg
     1201 aactggcggt cgtcctcaaa ccggctacag ataaggcgca ggctgaacga ccgttcgtca
     1261 aagtcatcac cgatccccag gggaattcga tcgaccacgg gcgcgaagtg gacctgaccg
     1321 aaaaaaatga aaccggcgat ttccgacacg agatcctccg cttgattgac aataccgagt
     1381 acaaattcga caccagccga tacttcgtct agccccatgc tatcttcgcg cgaccgatcg
     1441 cttgacagag tctgacaccc cagctattct cgcaacggtt atagaattcg catcgtttac
     1501 caacaaggag cgcgtgtatg cacacactgg gaatcaattt gggacggatg acccgcccct
     1561 tggtcgccgc gggcatgttg ctacttgcat cccttcctgc cgcacaggct gccgaaccct
     1621 tcaaaatggg catcgtcgat cctcaggccg tcctggagaa atcaaaagcc ggcaaaaaag
     1681 ccttggacac gctcaaagaa tatgtcaccg cccgacagaa gttattggcc gcggatgagg
     1741 aggagttgcg ccgcac
//
LOCUS       DATIPF010000190         1754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1713140, whole genome shotgun sequence.
ACCESSION   DATIPF010000190 DATIPF010000000
VERSION     DATIPF010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713140"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VLL06_04845"
     CDS             complement(<1..837)
                     /locus_tag="VLL06_04845"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HSS30327.1"
                     /translation="MKIPARAKITALGTYVPPRVLTNADLEKMVDTTDEWILERVGIR
                     ERHIVEKGVGASDLAVAAVKDLVEKHPFDLQEVDLIVVGTVTPDMMYPSTACLVQHKL
                     GIENTWGFDVSAGCSGFVYALNTGVKFVESGQYKKVLVIGSDVNSSMTDYTDRAVCII
                     FGDGAGAVLLEPSEDGEGVIDHVAQIEGVGGQFLFMPGGGSLNPASHATVDQKMHYIH
                     QDGKNVFKYAVKKMAEMTERILERNELAGKDIDCFIAHQANRRIITATADRLGMPMEK
                     VII"
     gene            complement(1056..1334)
                     /locus_tag="VLL06_04850"
     CDS             complement(1056..1334)
                     /locus_tag="VLL06_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30328.1"
                     /translation="MIAHGSSPQQAPHVTIRVSGKLFQGHLSYLDQLVQSAGECRLWP
                     LLSLAALEELDRHALFYLMDGEDREFGIVSCPNFIREWMEHEREGKAA"
     gene            complement(1502..1598)
                     /gene="ffs"
                     /locus_tag="VLL06_04855"
     ncRNA           complement(1502..1598)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VLL06_04855"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT          407 a          518 c          423 g          406 t
ORIGIN      
        1 gatgatgacc ttctccatcg gcattcccaa acgatccgcc gtcgctgtga tgatccgccg
       61 gttcgcctga tgcgcaataa agcagtcaat atccttgccc gccagctcat tgcgctcaag
      121 gatccgctcc gtcatctctg ccatcttctt tactgcatac ttgaagacat tcttgccatc
      181 ttgatggatg taatgcatct tctgatcgac cgtcgcgtgc gatgctggat tcaaactccc
      241 tccaccaggc atgaagagga actgcccgcc caccccctcg atttgcgcga cgtggtcaat
      301 cacaccctcg ccatcctcgc tcggttccag taaaaccgcg cccgcgccat cgccaaaaat
      361 gatgcacacg gcccgatcgg tatagtcggt catcgacgag ttcacgtccg atccgatcac
      421 cagcaccttc ttgtactgtc ccgattcgac gaacttcact cccgtgttca aggcgtatac
      481 gaaaccggaa caacccgctg atacgtcgaa cccccatgta ttctcgatgc cgagtttgtg
      541 ctgtactagg caggctgtcg acggatacat catgtccggc gtcaccgtgc ccacaacaat
      601 gagatcgact tcctgaagat caaagggatg cttctccacc agatccttca ctgcagctac
      661 ggcaaggtcg ctcgcaccca ctcctttttc gacaatgtgg cgttcgcgga taccaacccg
      721 ttcgaggatc cactcgtcgg tggtatccac catcttctcc aggtcagcat tggttagaac
      781 tcggggcggg acgtaggttc caagcgcagt aatttttgca cgagcgggta ttttcaattg
      841 gggggctccc ggccaacagg aaacctagta ttttgaggga ttcgaccgag aatgtctaac
      901 ccgaatcgtg aactgccaga ttttggcgct gtttgctcac aacctcgaat caatgcggct
      961 tagccgctga gcaccgcttg cgccaccgta gaagagcggc ccttcagggg ccgcgttggg
     1021 cgcttaaatt cagccggggc ccctggggtt tcaactcatg cagccttccc ttcccgctca
     1081 tgctccatcc attcacgaat gaaatttgga cacgacacaa tgccaaactc ccggtcttcc
     1141 ccatccatca aataaaacaa cgcatgacga tcgagctctt cgagtgcagc caggctcagc
     1201 agaggccaca atcgacattc tcccgccgat tgcaccaact ggtcgagata gctcagatgg
     1261 ccttgaaaca actttccgga aactcgaatc gtgacgtgcg gggcttgttg aggggacgaa
     1321 ccgtgagcga tcatgtttct ccttcggggc gacagaatct tgacggcgtc tgctttctga
     1381 cgactgactt ctatagacgg cccagaaaga cgtttcgtgc gaaaagagtg gtaaagaaaa
     1441 tgtaaaacta gataatcaga ggttggcgga ccttacgagg ttgcttggga aaaataaagt
     1501 caggtaccgg ggactcactg cacacgcaag agcccgttac cgttgcttcc ttccggacct
     1561 ggcggggttg gcgagagtac gtcgcgtgag accgatacct gacctgaatc attctagcat
     1621 tttgatcaca gatcaaaatc gcagcccaat cattctcaaa ttcgttacca gatgcccccg
     1681 aacattgatg atcgcaattc gctcgtttcc cacgagcggg aaacatcgtt ttcaacatgg
     1741 aagcaccgtt tttg
//
LOCUS       DATIPF010000191         5429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_656097, whole genome shotgun sequence.
ACCESSION   DATIPF010000191 DATIPF010000000
VERSION     DATIPF010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656097"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            355..864
                     /locus_tag="VLL06_04860"
     CDS             355..864
                     /locus_tag="VLL06_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30329.1"
                     /translation="MAKKTTSARKQGTTRNWIAYLCESLVTSGVMPTWEAATYLTENL
                     FGEKPNPRLLSATNAYEVTSQFLQRLYHPLVEAASRDIALPDGASLHVFTDISLTGQS
                     AVLVVYMSGNTPPHQLLMLDAAKAWELVFTDSEDVNRWAEERYRWVKSALTAAIGKPA
                     RQAILAEVS"
     gene            complement(910..4599)
                     /gene="metH"
                     /locus_tag="VLL06_04865"
     CDS             complement(910..4599)
                     /gene="metH"
                     /locus_tag="VLL06_04865"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HSS30330.1"
                     /translation="MSASIDALLQQRILVLDGAMGTMIQQRKLDEAAFRGERFKEWKK
                     DLRGHNDLLNLTQPAVIEEIHRQYFEAGADIVETNTFNAQAISLADYGMESLAYEIAK
                     AGAGCARRAAEHVASTEAGRVCFVAGAIGPTTKTSSISTDVNNSAARGTTYDELVKVY
                     YEQVRGLVDGGVDLLLVETIFDTLNAKAAFFAIDQFFDDRHIKLPLMASVTFIQAGSN
                     RGVTGQTIEAFWNSISHVPLLSVGINCALGPKEMRPLIEELSQLAPISVSCHPNAGLP
                     NPLLPTGFPETPDSLAPQLKEWAQNGWLNIVGGCCGTTPGHIKLIAEAVRSLPPRIPP
                     SVEPHTRLSGLEAVTIRPESNFVNIGERTNVTGSPAFSKLILGGDYEAALSVARQQVE
                     GGAQIIDINMDEGMLDSQAAMQKFLRLVASEPDISRVPIMVDSSKWDILETGLKDIQG
                     KPVVNSISLKEGEAKFLQQAKLVHRYGAAVVVMAFDERGQADTYERKIEVCKRAYRLL
                     TERIGFPAQDIIFDPNILTVATGIEEHNNYAVNFFDATRWIKQHLPLAKVSGGVSNIS
                     FSFRGNNRVREAMHAAFLYHAIKAGLDMGIVNAGQLAVYEEIPKDLLELVEDVLLNRR
                     PDSTERLVTFADTVKQQGKVAVKDNEWRSGTVEERLSHALVKGMTDYIEQDIEEARQQ
                     YAKPLHVIEGPLMAGINIVGDLFGSGKMFLPQVVKSARVMKKAVAYLMPFMEAEKQKS
                     GASSSNGKILLATVKGDVHDIGKNIVGVVLGCNNYEVIDLGVMVPCEQILAAAREHGV
                     AMIGLSGLITPSLDEMAHVARELKREGFDLPLLIGGATTSKAHTAVKIAPAYDHSVVH
                     VLDASRAVGVVGSLVNADLRADFATKVRADYDQIRQTHQDKGAKSLWPLARARANRFP
                     SEWAEVNIPTPAFTGVKVVPQQPLDQLIPYIDWSPFFHTWELRGRYPTILEDPVVGSK
                     AKELYDDALALLRKIVDQRLLTANGVYGFFPANSLGDDIELYTDGRRTEVLTTVHTLR
                     QQSEKPQGQYSFALADFVAPKESGRADYVGAFAVTTGIGVDLLCREFERDHDDYNSIM
                     TKALADRLAEAFAEWLHKQAREAWGYGKSEQLTSEDLIREKNRGIRPAPGYPACPDHT
                     EKRPLFNLLQAEQNTGITLTESFAMWPAAAVSGFYFAHPEAKYFAVGKIGKDQVEDYA
                     RRKGMDLRTIERWLSPNLNYEPSGE"
     gene            5019..>5429
                     /locus_tag="VLL06_04870"
     CDS             5019..>5429
                     /locus_tag="VLL06_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30331.1"
                     /translation="MLLRLSIHTIFSSLLLLLLTTGIWSQPSTVFAAAYFEDGYLGLT
                     QTELHQKLGMPQAVRDRKSELRVFTYYAITDWEKYFQKVVSPENGEDVYTFTRDGIEV
                     RYSFSYTVDPNDNSENRTLFVRLVDIEFSPTVPLT"
BASE COUNT         1158 a         1649 c         1389 g         1233 t
ORIGIN      
        1 ccgatcaatc cgcacacaca ctgattccca aaccctagga atctatccgc gtcatgccat
       61 gctcctgcgc agacgatccg cagacatctg catcgttccc ggtctgaaca catcccccac
      121 acattccagc gaatagacgt ccggtcttcg acactgcagt cacgcctctg tcaacgtctc
      181 cttcacctcg caagaacgaa tgactcggac agactcccgg cttccttttc gtcatttcgt
      241 tgaccggttt tccaccgacc gatatacttt cctcatctca tcaggtacag actcacgcac
      301 agattctcaa accctgtacc cacacatcgc acgatttcaa aagggttgcc gactatggca
      361 aagaaaacga cctcggcccg caaacaggga accacgcgca attggatcgc ctatctatgc
      421 gaatccttgg tcacctcagg agtcatgccg acctgggagg ctgccaccta tctcacagag
      481 aacctgtttg gagaaaaacc caatccccgt ctcttaagtg ccaccaatgc ctacgaagtg
      541 acgtcccaat ttcttcagcg gctgtatcat ccactcgttg aagccgcctc tcgcgacatc
      601 gccttgccgg atggcgccag tctgcacgtg tttacggata tcagtctcac cggacaatct
      661 gccgtgctcg tcgtctatat gtctggcaat acgcctccac accagttgct gatgctggac
      721 gcagccaaag cctgggaact ggttttcact gattcagagg acgtgaatcg ctgggcggaa
      781 gaacgctatc gttgggtgaa gtcagcgctg actgcggcga ttggaaagcc tgcccgccag
      841 gctatcttgg cagaagtctc ttaacgggcc ttgttatcca gatactcgct caccctgtcc
      901 cctcacctct tattctccac tgggctcgta gttcagattg ggcgagagcc agcgctcgat
      961 cgtgcgaagg tccatcccct tgcggcgggc atagtcttcc acttgatctt ttccaatctt
     1021 ccccacggca aagtatttgg cctcaggatg ggcgaaatag aatccgctga ccgccgccgc
     1081 aggccacatc gcaaagcttt cggtgagcgt gatgccggta ttctgctcgg cttgtaggag
     1141 attgaacagg ggacgtttct ccgtatggtc cgggcaggcc ggatagccag gggccggtcg
     1201 aataccccgg ttcttttcgc ggatcaggtc ttcactcgtc agctgctcgc tcttgccgta
     1261 tccccaggct tctcgcgctt gcttatgcag ccattccgca aaggcttcgg ccagacgatc
     1321 ggccaaggct ttggtcatga tggaattgta gtcgtcatgg tcacgctcga actcccgaca
     1381 aaggagatcg actccgattc ccgtcgtgac ggcaaaggct ccgacatagt cagcgcggcc
     1441 ggactctttc ggtgcgacaa aatccgccag tgcaaaactg tattgcccct gcggtttttc
     1501 cgactgttgg cgcaaggtgt ggacggtcgt caagacctcc gtgcgacggc cgtcggtata
     1561 cagctcgatg tcatccccca gactgttggc ggggaaaaac ccatagaccc cattggcggt
     1621 gagtagccgt tgatccacga tctttcggag caacgccaat gcatcgtcat acagttcctt
     1681 ggctttcgag ccgacaaccg gatcttccag aatggtcggg taacgtcccc gcagttccca
     1741 cgtgtgaaag aacggcgacc agtcgatata gggaatcagc tgatccaacg gctgctgcgg
     1801 caccaccttt accccggtga aagccggtgt gggtatgttg acctcagccc actcggatgg
     1861 gaatcgattg gctcgtgccc gtgccaaagg ccagagagac ttggctccct tgtcctgatg
     1921 ggtttggcgt atctgatcgt agtcggcgcg aaccttcgtg gcgaagtcgg cccgcaagtc
     1981 ggcattcacc agactaccga caacccccac agctcttgat gcatccagca catgcaccac
     2041 ggagtggtcg taggccgggg cgattttcac ggcggtatga gccttactcg tggtggcccc
     2101 accaatgagt aacggcagat caaagccctc acgcttcagc tcacgtgcca cgtgtgccat
     2161 ttcgtcaagc gagggggtaa tgagtccgct cagcccgatc atcgcaaccc catgttcgcg
     2221 tgcagcagct aagatctgtt cacagggcac catcaccccg agatcaatca cctcgtagtt
     2281 attacatccc aggaccaccc cgacaatgtt cttcccgatg tcatgcacat cgcccttgac
     2341 ggtggcgaga aggatctttc cattcgaact cgaggcccct gatttttgct tctccgcctc
     2401 catgaaaggc atcaagtagg ccacggcttt cttcataacc ctggcgctct tcaccacttg
     2461 cggcaaaaac atttttccgg atccgaacag gtcgcccacg atgttgatcc cggccatcaa
     2521 gggcccttcg atcacatgca ggggcttggc atactgctgt cgagcttcct cgatgtcctg
     2581 ctcgatgtag tcggtcatgc ctttgaccag tgcatgggag aggcgctcct caaccgtgcc
     2641 cgaacgccac tcattgtctt tcaccgccac tttgccctgc tgcttgacgg tgtcggcaaa
     2701 ggtcaccagc cgttctgtcg aatcgggacg gcggttcagc agcacatctt cgaccagttc
     2761 caggagatcc ttaggaatct cttcgtacac cgccaactgt ccggcattga cgatccccat
     2821 atcgagcccg gctttaatgg cgtgatagag aaacgccgcg tgcatcgcct cgcgcacgcg
     2881 attgttcccg cggaacgaga aggaaatgtt gctgacaccg ccactaacct tggccagcgg
     2941 aagatgctgt ttgatccatc gagtcgcgtc gaagaagttc accgcatagt tgttatgttc
     3001 ctcaataccg gtcgccacag tgaggatgtt cgggtcgaaa ataatgtctt gggccggaaa
     3061 cccaatgcgc tccgtgagga gacgataggc ccgcttacag acctcaatct tgcgctcata
     3121 ggtatcagcc tggccacgct cgtcgaaggc catcaccacc acggcagccc cgtagcgatg
     3181 cacgagtttg gcctgctgga gaaacttggc ttcgccttct ttcaggctaa tactgttgac
     3241 gacgggtttg ccctggatgt ccttcaaacc tgtctcgagg atgtcccact tcgagctatc
     3301 gaccatgatc gggacgcgag agatgtccgg ttccgacgcg accaatcgta agaacttctg
     3361 catggcagcc tgggagtcga gcatgccttc atccatattg atatcgatga tctgagcacc
     3421 cccttcgacc tgttgccgtg ccaccgaaag cgcggcttca tagtcaccgc ctagaatcag
     3481 tttggaaaat gccggtgacc ccgtgacgtt cgtccgttcc ccgatattca caaaattcga
     3541 ttccggccta attgtcaccg cttccagccc gctgagcctg gtatgaggct cgacggaagg
     3601 cgggatgcga ggaggaagac ttcgtacggc ttcggcaatc agcttgatgt gcccaggtgt
     3661 cgtcccgcaa cagccaccga caatattcaa ccagccattc tgcgcccatt ctttcaattg
     3721 gggcgccagg ctatcgggtg tctcggggaa ccccgtcggt agcaacggat tcggcagtcc
     3781 ggcattcgga tggcagctca cagaaatcgg agccagctgc gagagctctt caatcaacgg
     3841 acgcatttct ttggggccta acgcacagtt aatgccgacg ctgagtaagg gcacatggga
     3901 aattgaattc cagaaggctt cgatcgtttg acctgtcaca ccgcgattgc tgccggcctg
     3961 aataaacgtc accgaagcca tgaggggaag cttgatgtgg cggtcatcga aaaactgatc
     4021 aatggcgaag aatgcagcct tcgcattaag cgtgtcgaaa atagtctcaa ccaacaggag
     4081 atcgactccg ccgtcgacca agccgcggac ctgctcatag tagaccttca ccagttcgtc
     4141 ataggtggtg ccgcgcgcag cagagttgtt gacatcggtt gaaatcgacg aagtttttgt
     4201 ggtcggtcca atcgccccgg ccacaaaaca tactcgacca gcctctgtac tcgcgacgtg
     4261 ctccgcagca cgtctggcac atcccgctcc ggctttcgca atctcatagg ccaacgactc
     4321 catgccgtag tcggccaggg aaatagcttg cgcgttgaac gtattggtct ccacgatatc
     4381 cgcgccggcc tcgaaatact ggcgatggat ctcttcgatc acggcgggtt gcgtgaggtt
     4441 cagcagatca ttgtgtcccc tcaaatcttt cttccactct ttgaatcgct cgccacgaaa
     4501 cgcggcctca tccagcttcc gctgctggat catagtcccc atggccccat caaggaccag
     4561 aatacgttgt tgcagcaagg cgtctatcga ggcactcata cgactggtct ccgcgatcga
     4621 atctacggtg ttggtgaagg aagttgggcc atcataaccg agaggttctt gcaccggcaa
     4681 gcaatcttcg cccgcattac tcacgaaggc ttctactaca accgccgata cgaaaatcag
     4741 aacttcttgc ggtattcttt cttcgcgcca ggatcataca aagggcgctc gaccccgcaa
     4801 gcgcggtggc gacgtacctg acggcgggcg ggctcgccgc tcggtcggtc aacatactgc
     4861 ttcaagtatg cctccactcc ctcacggctc cgtgcgcccg tctcgcgtgg cgtctcagcg
     4921 gtttcgtgac gaactccgtg agtaatgcag gcttgtcctc gcgccttgac aggagggaat
     4981 gctgccgata cgatgccgag acatgctgga gataaattgt gctcctgcgt ctttctattc
     5041 ataccatctt ctcatcgctg ctgcttcttt tattgaccac tggaatctgg tctcagcctt
     5101 ccacagtctt cgccgcagcc tattttgagg atgggtacct cgggctcaca caaacggaac
     5161 tccaccaaaa actcgggatg ccgcaggctg tgcgcgatcg aaagtccgag ttgcgcgtct
     5221 ttacctacta tgccatcacc gattgggaaa agtatttcca gaaggtcgtt tcgccggaaa
     5281 atggcgaaga cgtctatacc ttcacccgtg atggcatcga ggtccgatac tcgttcagtt
     5341 acaccgtcga tcccaacgat aacagtgaga accgaacgct attcgttcgg ctcgtcgata
     5401 ttgagttctc ccccaccgtt ccactcacc
//
LOCUS       DATIPF010000192         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_568429, whole genome shotgun sequence.
ACCESSION   DATIPF010000192 DATIPF010000000
VERSION     DATIPF010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568429"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VLL06_04875"
     CDS             <1..407
                     /locus_tag="VLL06_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HSS30332.1"
                     /translation="GLQQWVTDLNRLYRQESALHERDLDPAGFEWVDCADAESSIVSL
                     LRKGKSTDDEILIVCNFTPVPRQNYRIGVSRNGFWKEVLNSDATQYGGSGWGNLGGLE
                     AVPIPLHGRAHSITLTVPPLAALYFKHPGRSS"
     gene            complement(487..1323)
                     /locus_tag="VLL06_04880"
     CDS             complement(487..1323)
                     /locus_tag="VLL06_04880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HSS30333.1"
                     /translation="MSNLIVFTDLDGSLLDGTTYSYEAATPALTALREQGVPLVLVSS
                     KTRAEMEPLRQRLDHHDPFIVENGGAVFIPHGLFDFPFERMRTRYPYQVIELGLPYHM
                     LREVLKQIEDAVETPLQGFGDLSVEDIMQATGLPYADAVLAKQREYDEPYLLQGPQTL
                     IEEVCRQIVTRGLRWTKGGRLFHLMGDNNKGEAASVLLRCYHRQQHMNGLTERIETVG
                     IGDSINDAPLLAMVDYPILVQKPDGSYDSDIHVAGMIRAPGIGPVGWNEAVLELLAQV
                     PP"
     gene            complement(1336..2043)
                     /locus_tag="VLL06_04885"
     CDS             complement(1336..2043)
                     /locus_tag="VLL06_04885"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30334.1"
                     /translation="MNDFISQALLKPLELLGRQLLTVLPNVLAMGIILLGGFFTAWAF
                     GFFVERLFRVIGFDHLCDRLGMNAALLRGGVKTDPSRIVGRSIYWTVIVLASIAALGA
                     LNLTPINQFAQSLLAYVPHLVTAAVILLAGYFLSNFTSRAVLIAAVNAGLPPARVIAA
                     CSRWGIQVLAGAMALEQLGIAQHIVVVGFGITWGGIVLAGAIAFGLGATDLAKGFLER
                     RLGNRPKSGTSDDLHHW"
     gene            complement(2036..>2715)
                     /locus_tag="VLL06_04890"
     CDS             complement(2036..>2715)
                     /locus_tag="VLL06_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HSS30335.1"
                     /translation="IWMTTEAIASGARVCQSFLGAKIHNPKDPSADLSAMLMQVMGAL
                     FALMEQHENLWSRQEGSQPVPLFGFPYEVGVEPVNVNVERMVSHYRQGLADLEPIWRQ
                     ILTDDTFTQLRTLQGTSNTDCRIPDELWVQVVYDAATAYRKRIMPREHLLKALTPLYL
                     GRTASFVYATQGLTSTEAEQKIEVLCQTFERMKSYLVERWQPPALQPAEPAQLHHTTA
                     AAGGDHE"
BASE COUNT          600 a          718 c          797 g          600 t
ORIGIN      
        1 gcggtcttca acaatgggtg acggacttga atcgtctcta tcggcaggag tcggcgctcc
       61 acgaacgtga tctggacccg gcggggttcg agtgggtgga ttgtgcagat gcggagtcca
      121 gcatcgtcag tctcctccgc aaaggaaagt ccacagatga tgaaatcttg atcgtgtgca
      181 acttcacacc ggttcccaga cagaattatc ggataggtgt ctcccggaat ggattctgga
      241 aggaggtctt aaatagcgac gccactcagt atggagggag cggatggggt aacctcgggg
      301 gcctcgaggc tgtcccgatt ccgctccacg ggcgcgctca ctccatcact ctgacagtac
      361 cgccccttgc agccctctac ttcaagcatc ctggccgttc gtcatgaaga atgattgtga
      421 gatatgttca agcatgtccg tccgtgggcg gggctcttta cttgcgggtt gggttcaggc
      481 ggtcccttat ggtgggactt gcgccaaaag ctccaggacc gcttcattcc atcccaccgg
      541 tcctattcct ggagcacgaa tcatccccgc gacgtgaata tcggagtcat acgatccgtc
      601 tggtttctgg acgagaatgg ggtaatccac catcgcgagc aggggggcat cattgatact
      661 gtccccgatc ccaactgttt cgatccgctc tgtcagtcca ttcatatgct gttggcgatg
      721 gtaacagcgt aggagcacgg aagcggcctc ccccttattg ttgtctccca tcagatgaaa
      781 taaccgtccc ccttttgtcc agcgaagacc tcgggtgacg atttgccgac agacttcttc
      841 aatcagcgtt tgagggcctt gaaggagata gggctcgtcg tactcccgtt gcttggccag
      901 aacggcgtcg gcatagggga gcccggtcgc ttgcatgatg tcctctaccg acaggtcgcc
      961 aaagccttgt aacggagttt caactgcatc ttcgatttgt ttcaggacct ctcgcagcat
     1021 gtgatagggg aggcctaatt cgataacctg gtagggatat ctggttcgca tgcgttcaaa
     1081 agggaagtcg aagaggccat gagggataaa taccgcgcct ccattttcga caatgaatgg
     1141 gtcgtgatgg tcgaggcgct gtcgaagtgg ttccatctca gcgcgtgttt tgctcgacac
     1201 cagtacgagc ggaacacctt gctcgcggag tgcagtgagc gctggtgtgg ccgcctcgta
     1261 ggaataggtc gtgccgtcca acagcgagcc gtccaagtct gtaaagacga tcagattgga
     1321 catggtatga caccgttacc aatgatggag atcatcgctt gtgccggatt taggtcggtt
     1381 ccccagacgt cgttccaaga atcccttggc gagatcggtg gcgccgagtc caaatgcaat
     1441 cgctccggcc agcacaattc ctccccaggt aatgccgaat cccacgacga cgatgtgttg
     1501 ggcaatgccg agttgttcca gcgccatggc tcctgcaaga acttggatcc cccagcggga
     1561 acaggcagca atcactcgag cggggggtaa tccggcgttg acggcggcaa tcaggacagc
     1621 ccgagaggta aagttcgaga gaaaatatcc ggccaggaga atgaccgctg ccgtgaccag
     1681 atggggcaca taggccagca gtgattgggc gaactggttg atcggagtca agttcagagc
     1741 gcctaacgcc gcgatcgaag ccaacacaat gactgtccaa tagatgctgc ggccgacgat
     1801 acgtgaggga tcggtcttga ctcctccacg caggagtgcc gcattcatcc ccagccgatc
     1861 acagagatgg tcgaatccga tgacgcggaa gagtcgctca acgaaaaacc cgaatgccca
     1921 cgcggtgaag aagcctccga gcaggataat gcccatggca agcacattgg gtaatacggt
     1981 cagcaactga cgtccgagga gttccaacgg cttgagaagc gcttggctaa tgaagtcatt
     2041 catgatcacc tcctgcggca gcggtcgtgt gatgcaactg agccggctcg gcgggttgga
     2101 gggccggagg ttgccacctt tcaaccagat aggatttcat gcgttcgaat gtctgacata
     2161 agacttcgat tttctgttct gcttcggtgg acgtcaggcc ttgagtggcg tagacgaacg
     2221 aggcggttcg ccctaagtac aggggggtga gcgctttcag aagatgctcg cgcggcatga
     2281 ttcgcttgcg gtacgcggtg gccgcatcat agacgacctg gacccacaat tcatcgggaa
     2341 tacgacagtc ggtgttcgat gttccttgga gagtccgtag ttgagtgaat gtatcgtcgg
     2401 tcaggatctg tcgccagatg ggttcaagat cagcaaggcc ttgtcgatag tgcgacacca
     2461 tccgctccac attgacattg accggttcga ccccgacctc gtagggaaac ccaaaaagtg
     2521 gaactggttg cgatccctcc tgccgggacc agaggttttc atgctgttcc attaacgcaa
     2581 agagggctcc catcacttgc attaacatgg cagagaggtc ggcagaagga tccttgggat
     2641 tatggatttt tgcgccgagg aagctttggc agactcgtgc accactggcg atggcctccg
     2701 tcgtcatcca gatat
//
LOCUS       DATIPF010000193         8582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1713237, whole genome shotgun sequence.
ACCESSION   DATIPF010000193 DATIPF010000000
VERSION     DATIPF010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713237"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VLL06_04895"
     CDS             complement(<1..478)
                     /locus_tag="VLL06_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30336.1"
                     /translation="MDRDQNGIDTESLRQTYGTLINLWTSGVRDYHSLLSDYLTANSI
                     FVAAIGFILARQPPTFLFTLLGIILCIFGILMVLQMAIVLGRFSAQIALWEWQLRGTE
                     LHTSWTQPQLFNRLHQYRDHHQSLEDHKNNPPVLRPTWALRQHRQWWARREFSFPIF"
     gene            complement(529..1050)
                     /locus_tag="VLL06_04900"
     CDS             complement(529..1050)
                     /locus_tag="VLL06_04900"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS30337.1"
                     /translation="MKLPIKISSRHRGKVLGLDSEAETMTLLGPTGEPLGTLSWEAVI
                     DFIQSSVKETRSHQAARNYPRSRLAAKVRYVTPDHKHFDSVTCEIGGGGIFIETHLPP
                     QLGTALTLELILPDDPTAPINAQGKVAWVRPGEEHYVFFPGMGVQFTEISESGRERLL
                     TMVKTLDHARQGS"
     gene            1408..1953
                     /gene="lexA"
                     /locus_tag="VLL06_04905"
     CDS             1408..1953
                     /gene="lexA"
                     /locus_tag="VLL06_04905"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HSS30338.1"
                     /translation="MLAKQFRQARVRLGYTQAKLAEVLQLSRMTISRYEAGTWEVPLS
                     VEMALDQLGSSQIPMLGMVAAGAPIEPITQAELVAVLPPLLRGGETFALRVKGESMKD
                     DGIFPGDLVVVRKQGTARNGQTVVALVNNEATIKKYYRKDGRIELHPANAAMKPIIVS
                     SEDEFCIQGIVAGVIRHFNES"
     gene            1962..2672
                     /locus_tag="VLL06_04910"
     CDS             1962..2672
                     /locus_tag="VLL06_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30339.1"
                     /translation="MALIQQQAIEFPFRTEDGGAVINAPRHLLAELRRSAVSTDQPKG
                     LVLLSGPSSVYRLGLCAAVDRALAGEPVLYLAGANVFDPFLVGRLARASRATPAHVLQ
                     QIHVSRAFTCHQMVRLVTDCLPSALRTYDARFVILAGPLDTLYDRSVPEPEAMRLFRA
                     MTEALQDMANQGVQVLCVSPFAPPESGGRSRFMMTLRAQARRAIDLREAEDGLWLQEE
                     GGPEPRRWMIPRLFWNRL"
     gene            2675..2965
                     /locus_tag="VLL06_04915"
     CDS             2675..2965
                     /locus_tag="VLL06_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30340.1"
                     /translation="MGRTLATFTQLVQQEIDSWSRYRRALRREDQQALDVLFAAARHH
                     ASAGAYLARETPFEVMLLSMLIEQQKHVFTLQQKVMTLETRMFHDSTQGMAS"
     gene            2988..5195
                     /locus_tag="VLL06_04920"
     CDS             2988..5195
                     /locus_tag="VLL06_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HSS30341.1"
                     /translation="MVVWLLCEDGRRIRTVDPFVPTCYLAASPSIREQVFRLLRRAPC
                     RLSTREVERYELGAPHPTPVLEVSVHAPAQFSSLTQQLIRSCPDAQFFHVDVSLPQRY
                     FYDRELFPLVHCEVDLTVENRIQSIQALESPWNTDYGLPPLTIMELSTDNPSPNPNHG
                     GRGSLVVRMDGEERALEGDDADVIQRLHQLLVRQDPDILLTDWGDSYLLPRLMTMADR
                     WRLPLALNRDPHRSIGTKRPRSYFSYGRILSHAGARTLSGRLHLDRHNSFALAEMGLA
                     GLIEQARVAKVDLQQMARTTTGTGITSMQLETACRDGLLIPYRKQEPEAFKSALELLQ
                     TDQGGLVFAPIVGYHEQVGELDFSSMYPSIMTQFNISPETVNCRCCRDEPTAQVPEIG
                     HHICLHRRGLVPRTLAPLLGKRARYKQLMAAATQPAARAVLNQRQTALKWLLVVSFGY
                     LGYKNARFGRIEAHESVTAYSRELLLQAKEVAERQGYRMLHAIVDSLWLVKPGAVRAD
                     YDRLAQAITERTELSIGVEGLYHWIGFLPSRVNPLMPVPNQFVGLFDDGAMKIRGIEI
                     RRSDAPFLVKRVQAEVLRCLSQARTLAGLSEQIPQALEIVHTYRRYLRDGHATVEELA
                     IAKSLSKEPQTYHHQTLLMIAAQELLARGVTLQAGDTIHYIITDVDAAYPPDRVRAVD
                     GLDGAWSYDVSAYDNLLLKAILVLLTPLGVTATSLNESTDPASRAAYNLPDRN"
     gene            5374..6240
                     /locus_tag="VLL06_04925"
     CDS             5374..6240
                     /locus_tag="VLL06_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30342.1"
                     /translation="MAPVLELDRQTASFVRQLEEQRKLEKTLLVERVRGTKEGLESVF
                     TDIKSRVAVLEKLNGSASASCIAPIGWSQLIFTGPASVSSPNTIGFGDRDETLCKLDQ
                     IRYLEDMSYLRLVSNPHYVEHMLPNKSAQARVGANPSMWDTLGYLHVGYTTMLQIPAA
                     MEPTTVSIMSRLNTLFNWVEANAVGDEWFAQVSATARLWVTYDGEFRQAPAAQFVRLL
                     ATPKQLRTHQDIASQAPSAALTMQVSASAGSGTTISVYESIELVATTPNGHAYVDGIF
                     SWEPLAVQLREG"
     gene            complement(6267..7538)
                     /locus_tag="VLL06_04930"
     CDS             complement(6267..7538)
                     /locus_tag="VLL06_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30343.1"
                     /translation="MPIENNFQHDEMSRKNPGERITVREATPTALSESASGLDAMAAL
                     GKRLSHTGVGLIVFMHGSILGTDVFGVQRLDELGGLKRGYSRGVAGLDALLALMRERS
                     NGIASLPGGLTPPLMNNDATKKLLDEHIGDAGNFTNAYMEQMKLSLNRGLDRPIHYIR
                     ELWSCEHHHLGRALAAVSMLKRLCDWCETYRLGQGDRILVQAHGQAGLVLALASNLLS
                     VASTSSRTRLCDLLSAYASDVDRSDITTTIQRVAPLLSNGTLLNGATLDVVTLGMPVR
                     YGWDPSGLGKLLHIVNHRNLRTDGKTWLSKMELPQITMEMPIAWGGDYVQELAVAGSD
                     AVPTTDAGKAANKAVWEIVEPFDGFERWLECARRAVRIPSEGLGILVDYRDSTGSTNV
                     RDHYFGHAVYTRLNMMLFNTTEIVQTLYQSP"
     gene            complement(7623..8189)
                     /locus_tag="VLL06_04935"
     CDS             complement(7623..8189)
                     /locus_tag="VLL06_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30344.1"
                     /translation="MKNRSGQLMVVLIAAGCSGLLPLHSYAADSGDVRAHAEQSFNQL
                     TQHTGQGDSSKAPAVLRRTVEDRNSYSSDQYLIGKGQGDMAKGRIICQRVSELSARTD
                     LAKQIRILVKEHMVDRVRERIGGAAEQDVEVTREEIVQEYLQGVKIVDRQIDEDSKTC
                     SATAVMLKNQIQPKPAPDHLDPTPTVIP"
     gene            complement(8193..>8582)
                     /locus_tag="VLL06_04940"
     CDS             complement(8193..>8582)
                     /locus_tag="VLL06_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30345.1"
                     /translation="PAPGSQTAPRTLNEITYHTPTAPPHIGNQPGVTYQLPSMSHQTP
                     TPGTRSTHALQQFLQQPQAGPGTPPVTNHLPPAMHQRHSPAFQHVPHVPSQMAPQGFG
                     GGGHHGGGGRGGGGGGHGHGGGGGRGR"
BASE COUNT         1716 a         2393 c         2582 g         1891 t
ORIGIN      
        1 aaaagatggg aaagctgaac tcgcggcggg cccaccattg ccggtgttgg cgaagtgccc
       61 aggtcgggcg taagaccggc ggattgttct tgtggtcttc aagagactgg tggtgatctc
      121 tgtactggtg aagtctgtta aataactgtg gctgcgtcca gctcgtgtgg agttcagtgc
      181 cgcgaagttg ccattcccac agggcgatct gggcggaaaa tcgtccgagt acgattgcca
      241 tttgcaggac catgagaata ccgaatatgc agagaatgat acccaagagc gtaaagagaa
      301 acgtgggggg ttggcgggcg aggataaacc caatcgccgc gacaaagatg gagttcgccg
      361 tcaagtaatc ggacaataaa ctgtggtaat cacggactcc tgacgtccag aggttgataa
      421 gtgtcccata cgtctgccgg aggctttccg tgtctatacc gttttggtct cgatccattc
      481 atgctccgat agaatcagtt cgggcctcgc tccccgtcaa gaaggggctc aactcccctg
      541 gcgtgcatga tcgagagttt tgaccatggt caacaggcgc tcccgtcctg actcggaaat
      601 ctcggtgaac tggactccca tccctggaaa gaacacataa tgctcctccc caggcctgac
      661 ccaggccact ttgccttgtg cattgatcgg cgcggtcgga tcgtcaggca gaatgagttc
      721 caacgtgagg gccgtcccta actgcggtgg caggtgggtc tcgatgaaga tcccgcctcc
      781 gccaatctcg caggtgacac tatcgaagtg tttgtggtct ggtgtcacgt agcgcacctt
      841 cgctgcgaga cggctgcggg gataattccg ggctgcttga tgggatcgag tctctttcac
      901 agagctctga ataaaatcta tgacggcctc ccacgacagg gtccccagcg gttcgcctgt
      961 cgggccgagc agcgtcatcg tttccgcttc actatccagc cccaagactt tgccccggtg
     1021 ccgcgatgag atcttaatgg gcaacttcat tatggtccct cctctcggtg ggaaacgaac
     1081 aggcccgccc tttccgtatg cattctcgat tcatcacgca tccggcccag gttctcagcc
     1141 ctgagttcca gaagaatgag tctttaacct tgagggtttt tgtggacgcc ctaggatatg
     1201 tcaaattccg cgagaaatcg accgtgcatc cggaaagtgt tcaacaaaga ttgtgatgcc
     1261 gagaaaaacg gtgtccggta cagtttctca agaaaaaccg cctcagatgt gggacgctcc
     1321 cctcctcatt gatactccca tccataggta ttaccctctc aatcaccccc ttgacagcgc
     1381 cggctatagc atgtaacatt ttgtgacatg ctggctaaac agttcagaca ggcgcgggtt
     1441 cggttggggt acacgcaggc gaagttggcg gaggtgctgc aactgagccg gatgacgatc
     1501 agccgctatg aagccggcac gtgggaggta cccttgtcgg tcgaaatggc gttggaccag
     1561 ttaggatcgt cgcagattcc gatgctcggg atggtggcgg ccggggcgcc gatcgaaccg
     1621 atcacgcaag ctgaactggt ggcagtgctg ccgccgttgt tgcgtggagg ggagaccttc
     1681 gcgttgcgtg tcaaagggga gtcgatgaag gacgacggta ttttcccagg cgatttggtg
     1741 gtggtgcgga aacagggcac cgcacggaac gggcagacgg tggtggcgtt ggtcaacaat
     1801 gaagctacga tcaagaagta ctatcgcaag gacggtcgga tcgaactgca cccggccaat
     1861 gcagcgatga agccgatcat cgtgtcgtca gaggacgagt tttgcattca gggaattgtc
     1921 gcaggggtga tccgccactt caacgaatca tgaggtactc tatggcgctg atacagcaac
     1981 aggcgattga gtttccattt cgcacagagg atggcggcgc ggtcatcaat gcgcctcgcc
     2041 atctgttggc cgaattgcgg cgaagtgcgg tgtcgaccga tcagccgaag gggttggtgc
     2101 tgttgtccgg accgtcgtcg gtctatcggc tgggcctctg tgccgcagtt gatcgcgcgt
     2161 tggccggcga accggtgttg tatctggccg gtgccaatgt cttcgacccg ttcttggtgg
     2221 ggcggctggc tcgagccagt cgggcgacgc cggctcacgt gctgcaacag attcacgtct
     2281 cgcgagcctt cacctgtcat cagatggtca ggctggtcac ggattgtctc ccgtcggcgt
     2341 tgcgcaccta tgatgcgcga ttcgtcattc tcgccggtcc gctcgacacg ttgtatgacc
     2401 ggtcggtgcc ggagcctgaa gcaatgcgcc tctttcgtgc catgacggag gcgctgcagg
     2461 acatggccaa tcaaggtgtg caggtattat gcgtgagccc gttcgcgccg cctgaatccg
     2521 gaggacgcag cagatttatg atgacgcttc gtgcgcaggc gcgccgggcg atcgacttgc
     2581 gcgaagcaga ggatggtcta tggctccagg aagagggagg gcctgaaccc agacgctgga
     2641 tgattccgcg tctgttctgg aatcggttgt aagtatggga cgcacgcttg ccacgttcac
     2701 gcaattggtc caacaggaaa tcgactcgtg gagccgctac cggcgtgcat tgcggcgcga
     2761 ggatcagcag gcactcgatg tgctgttcgc cgctgcccgc catcatgcgt cggccggcgc
     2821 ctatctcgcg cgtgagacgc cgttcgaggt gatgctgctg tccatgttga ttgaacaaca
     2881 gaaacacgtc ttcacgttgc aacagaaagt gatgactctt gaaacccgca tgttccacga
     2941 ctcgactcag gggatggctt cttgatgtct acccggcaca agagggcatg gtcgtctggc
     3001 tcctctgcga ggacggtcgc cgcatcagga ctgtcgatcc cttcgtgccg acctgttatc
     3061 tggcagcttc gccatccatt cgcgagcagg tattccggct gctccgccgc gctccctgtc
     3121 gtctcagcac cagagaagtg gagcgatacg aactcggtgc acctcatcca accccggttc
     3181 tggaggtctc ggttcatgct cccgcccagt tctcgtccct cacgcaacag ctgatccgtt
     3241 cctgccccga tgcgcagttc tttcacgtgg atgtgtcgct gccgcagcgc tacttttacg
     3301 acagagaact ctttcctctc gtccattgcg aggttgacct cacagtcgaa aaccggatcc
     3361 agtcgattca ggcgctggag tcaccctgga acacggacta tggtctcccg ccgctcacga
     3421 tcatggagct gtcgacggac aatccgtcgc ccaacccaaa ccatggaggg cgtggttcgc
     3481 tcgtggtgcg gatggatgga gaagagcgtg cgttagaagg cgatgatgcc gatgtgatcc
     3541 agcgccttca ccagctgctg gtgcgacagg accccgatat tctcttaacg gactggggcg
     3601 attcctacct tctgccgcgt cttatgacca tggcggatcg ttggcggctg ccgcttgcgc
     3661 tcaatcgcga tccccatcga tccatcggca cgaagagacc ccgttcgtat ttttcgtacg
     3721 gccgcatcct gtcccatgca ggcgcccgta cgttgtcggg acggttgcac ctcgatcggc
     3781 acaattcctt tgcgctggcg gaaatgggcc tggccggcct tattgagcag gcccgtgtcg
     3841 caaaggtcga tctgcagcag atggcccgca cgacgacagg gacggggatc acgtcgatgc
     3901 agctcgaaac agcctgtcgt gatggcctgc tgattcccta ccggaagcag gagccggaag
     3961 ccttcaagtc cgcgctcgag ttgctgcaga ccgatcaggg cggattggtg tttgcgccga
     4021 tcgtcggcta tcacgaacag gtcggcgaac tggatttctc ctcgatgtat ccctccatca
     4081 tgacgcagtt caacatctcg cctgagacag tcaattgtcg gtgctgccgc gatgaaccga
     4141 ccgcgcaggt accggagatc ggccatcaca tctgcctcca tcgccgggga ttggtgcctc
     4201 gcacactggc tccgttgctc ggcaagcgcg cgcgatacaa acagctcatg gccgcggcga
     4261 cacagccggc ggcgagggcc gtgctcaatc aacggcaaac ggcgctcaag tggctgctcg
     4321 tcgtctcgtt cgggtatttg ggatacaaga atgcccggtt cggtcggatc gaagcgcatg
     4381 agtccgtgac cgcctacagc cgtgagctgt tgttgcaggc taaggaagtt gccgagcggc
     4441 agggctatcg catgctgcat gcgattgtag actcgctctg gctcgtcaag cctggtgcgg
     4501 tcagagcaga ctatgacaga ctcgcgcagg cgattaccga gcgaacagag ctctcgattg
     4561 gcgtagaagg gctctatcac tggatcgggt ttcttccgtc acgggtcaat ccgctcatgc
     4621 cggtgccgaa tcagttcgtg ggcctgtttg acgatggcgc catgaaaatc cgcggcatcg
     4681 aaatccgtcg cagcgatgcc ccgttcctcg tcaagcgggt acaggcagaa gtgttgcgct
     4741 gtctgagcca ggcacggacg ctggccggat tgtccgaaca gattcctcag gcattggaga
     4801 tcgtccatac ctatcggcgt tatctccgag atggtcatgc cacggttgag gagttggcca
     4861 tcgcgaaatc gctctcgaaa gagccgcaga cctatcacca tcagaccctg ttgatgattg
     4921 cggcccagga actgctcgca cgaggcgtga cgctccaagc cggtgacacg attcactaca
     4981 tcattacgga tgtcgatgcg gcctatccgc cggaccgggt gcgcgcggtg gatggactcg
     5041 atggagcctg gagttacgat gtctcggcct atgacaatct gcttctgaag gcgatcctcg
     5101 tgctgttgac ccccttaggt gtaacggcga cctcgctgaa cgagtcgacc gatcctgcca
     5161 gccgtgctgc gtacaacttg cctgatcgta actaatcccc atcgtggcct agcagtgccg
     5221 tccaaacctg tccatatcgt cggtccttgc ctgcggggat cgattgaata gcgcctgttt
     5281 gtatgggttt agcaatggca ctcatcttgc gcaatccctt ggtttccatg tgcggcgtag
     5341 agggcatgag atgaacatga aaagggggat ccgatggcac cagtgttgga actcgatcgg
     5401 cagacagcct cgttcgtgcg gcaactcgaa gaacaacgga aactcgaaaa gactcttttg
     5461 gtcgaacggg ttcgtgggac gaaagaaggg ctggagtcgg tcttcaccga catcaagagc
     5521 cgcgtggcgg tgttggagaa actaaacgga tcggcaagtg ccagttgcat tgcgccgatc
     5581 ggttggagcc aattgatctt taccggaccg gcgagcgtgt cgtcgccaaa tactatcggg
     5641 ttcggggatc gggatgaaac cctttgcaag ctcgaccaga ttcgctatct cgaagatatg
     5701 agttacctcc gtcttgtgtc gaatccccat tatgttgaac atatgttgcc gaataaatct
     5761 gctcaggcgc gtgtcggggc gaatccatcc atgtgggaca cgttggggta tctgcatgtc
     5821 gggtatacga cgatgttgca gattccggct gcgatggagc cgacgacggt gtcgatcatg
     5881 tcgcggttga atacgctgtt caattgggta gaggccaatg cggtcgggga tgaatggttc
     5941 gcgcaggtgt cggcgacggc gcggctgtgg gtgacctacg acggcgagtt tcgccaggct
     6001 ccggccgcgc agtttgtgag actcctcgcg acaccgaagc aactgcgaac gcatcaagat
     6061 atcgcgtctc aagctccgag tgcggcgttg accatgcaag tctcggcatc agccggcagc
     6121 ggtacaacga tcagcgtata cgaaagtatt gaactcgttg ccaccacgcc gaacggccat
     6181 gcctacgtcg acggcatatt ttcctgggag ccgctggcgg tacaacttcg agaaggatga
     6241 cagcgggtgt cgatagagat gaaacattac ggggattggt agagagtctg tacgatttct
     6301 gtggtgttga agagcatcat gttgagtcgg gtatacaccg catggccgaa gtagtggtcg
     6361 cgcacgttcg ttgatccggt agagtctcta tagtcgacga gaatccccag cccctcactg
     6421 ggaatgcgga cggcacgacg ggcacattcg agccatcgtt caaacccatc aaacggctcc
     6481 acgatttccc atacggcttt attggctgcc ttccctgcat cggttgtcgg cacggcatcg
     6541 ctgccggcca cggccagttc ctgtacatag tctccacccc atgcgatcgg catttccatc
     6601 gtgatctggg gcaactccat tttcgagagc caggtttttc catcggtccg aaggtttcga
     6661 tggttgacga tgtgcagtag tttgcccaat ccggacggat cccacccgta acgtaccggc
     6721 atgcccaatg tgacgacgtc cagtgtggcc ccattgagga gggtgccatt cgacaacaat
     6781 ggcgcgacgc gttggattgt cgtcgtgatg tctgaccggt cgacgtccga ggcatatgcc
     6841 gacaggagat cacacagccg tgtccggctg ctggtcgaag caacagatag gaggttcgag
     6901 gcgagggcaa ggactaaccc ggcctgccca tgagcttgca cgagaattcg atctccctgg
     6961 cccagcctat aagtctcgca ccagtcgcag agacgtttga gcatagagac tgccgccaat
     7021 gcacggccga ggtgatggtg ttcacaggac cagagctcgc ggatgtaatg gattggacga
     7081 tccaggcctc tgttgagtga cagtttcatc tgttccatat aggcgttcgt gaaattgcct
     7141 gcatctccaa tgtgctcatc gagcagtttc ttggtggcgt cattattcat aagcgggggg
     7201 gtcaagccgc cgggcagtga ggcgatgcca ttactgcgtt cacgcatcag agccaatagg
     7261 gcatcgaggc ccgccacgcc gcgtgagtag ccacgtttga gcccgcccag ttcatccaat
     7321 cgttgcaccc caaagacgtc ggttcccagg atggagccgt gcatgaagac gatcaggccg
     7381 acgccggtgt gcgagaggcg cttgccgagc gcagccatcg cgtccaggcc ggaggccgat
     7441 tctgataaag cagtcggagt ggcctcgcga accgtgatcc gctcgccagg attctttcga
     7501 gacatttcgt cgtgttgaaa attgttctcg attggcatgg gggagttaca tacccgaagt
     7561 gagtgaagga ctgcaacgca agatcagcat cctcaggcta agcccactat gaaacactgg
     7621 gtttatggaa tgaccgtcgg ggtagggtcg aggtggtcgg gtgctggttt gggctggatc
     7681 tggttcttga gcatgacggc ggtcgcgcta caggtcttgc tgtcctcgtc gatctggcga
     7741 tcgacgatct tcacgccctg taaatactct tgaacgattt cctcccgtgt gacttcgaca
     7801 tcctgctcgg cagcgcctcc gatccgctcg cgaactcggt cgaccatgtg ctctttcacc
     7861 aaaatacgaa tctgtttggc cagatcggtt cgggcagaga gttccgatac gcgctggcag
     7921 atgatacgcc ctttcgccat gtcgccctga cctttgccga tgaggtactg gtccgatgaa
     7981 tagctgttgc gatcttcaac cgtgcggcgc aacacagccg gcgctttcga ggaatctcct
     8041 tgtcctgtgt gctgtgtgag ttggttaaac gattgctcag cgtgggctcg aacatcgcct
     8101 gaatccgcag catacgaatg cagcggtagc aagcctgagc agcctgcggc gatcaacaca
     8161 accatcaact gaccacttct gttcttcacg cgctacctcc ctcgtcctcc gcccccacca
     8221 tgcccatgcc cgccccctcc gcctcctcgg ccgcctcccc catgatgccc gcctccgccg
     8281 aacccttgag gtgccatctg agagggtacg tgtgggacat gctggaaggc cggcgaatgg
     8341 cgctgatgca tcgccggagg taagtggttt gtcacaggcg gtgtgccagg tcctgcctgc
     8401 ggctgctgta aaaactgctg aagtgcatga gttgaccgtg tgccgggagt cggagtctga
     8461 tgcgacatgg aaggcaactg ataggtgaca ccgggttgat tgccaatgtg tggtggcgct
     8521 gtcggtgtgt gatatgtgat ttcgttcaac gttcgcggag ccgtctgcga tcccggcgcc
     8581 gg
//
LOCUS       DATIPF010000194         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1362388, whole genome shotgun sequence.
ACCESSION   DATIPF010000194 DATIPF010000000
VERSION     DATIPF010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362388"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /gene="trmFO"
                     /locus_tag="VLL06_04945"
     CDS             complement(<1..1120)
                     /gene="trmFO"
                     /locus_tag="VLL06_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HSS30346.1"
                     /translation="MREDIVIIGGGLAGSEAAWQAANRGAKVTLYEMRPKEMTQAHKT
                     GGLAELVCSNSLGSADLLNAPGILKEEMRRLNSLIIQVADEVRVPAGSALAVDREQFS
                     LKITQALEGHPNIRILHEEITEIPTDCLCILATGPLTSDKLSRAIAQLTHTKHLYFYD
                     AISPIVDADSINMDVVFLASRYDKGGDDYLNCPMDEPAYNAFYDALLAAEKVQPKEFE
                     KTPYFEGCIPIEVMAERGRQTMQFGPLKPVGLENPKTGARAYAVVQLRTENAHRSCYN
                     LVGFQTKLTYGEQKRVFRMIPGLEQAEFLRYGSLHRNTFINSPQLLRDTLQFKARGTL
                     FFAGQLVGVEGYTDSAAMGGLAGINAARGLSGLPLVTPP"
     gene            1266..1340
                     /locus_tag="VLL06_04950"
     tRNA            1266..1340
                     /locus_tag="VLL06_04950"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Val,seq:cac)
     gene            1508..1852
                     /locus_tag="VLL06_04955"
     CDS             1508..1852
                     /locus_tag="VLL06_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30347.1"
                     /translation="MMRTTALALGILLLATAVGCAENRQQQALADADTGYTPPVSIYS
                     VMDPTSLIYTDVAAGASANDNPWRWAGFFLHPVGVAIDYGVNRSFYTISSKMPYLFGY
                     TAEDSMLDSQRR"
     gene            2136..>2878
                     /locus_tag="VLL06_04960"
     CDS             2136..>2878
                     /locus_tag="VLL06_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS30348.1"
                     /translation="MSKSDFLKTHPASTLKPQNWGTGQELVLPADRSPSQGDESYFFD
                     EDGLLVGTVFTFPSGLDLNPYPVLRHTLTLLKPTLEFYLNVAQLSSMTSMNSSALYET
                     GDEKTTTQYLVLGTREQPVLLQASMTIDPYVRLFSPYRREFLERLRYPSGRKPGQQLD
                     SQGAEDKEHFPSLQQFARGQTAQLSYCGPQNYEIASSAYQKALASGFTNKVWQAEAHH
                     KLGLAWEGMGQYEKAKTEMLQSLTIRPNTP"
BASE COUNT          660 a          829 c          735 g          654 t
ORIGIN      
        1 gaggcggcgt gaccaacggc aatcccgaaa gaccacgggc cgcgttgatt cctgccaacc
       61 ctcccatcgc agcggaatcg gtatagccct cgacgccgac cagctgtcct gcaaaaaaga
      121 gagtcccgcg cgccttgaac tgcagggtat cacgcagcag ttggggagaa ttaataaagg
      181 tgttgcggtg caggctgccg tagcgcagaa actcggcctg ctcaagaccg ggaatcatgc
      241 gaaacacccg cttctgttcc ccgtaggtca actttgtctg aaagcccacg agattgtaac
      301 aggagcgatg ggcattttcc gtccggagct gcaccacagc ataggctcga gcacccgttt
      361 ttggattctc aaggcccacg ggcttcaaag gaccgaactg catggtttgg cggccccgtt
      421 cggccatcac ttcgatggga atacaacctt caaaataagg agtcttttcg aattctttcg
      481 gttggacttt ctctgccgcg agcaatgcgt catagaacgc attgtaagcc ggttcgtcca
      541 tgggacagtt gagatagtca tccccgccct tgtcgtaacg ggacgcaaga aatacaacat
      601 ccatattgat ggaatcggca tccacaatcg gagaaatcgc atcgtaaaaa tacaaatgct
      661 tggtgtgggt caattgggca atcgcccgag acagtttgtc ggacgtgagc gggccggttg
      721 ccaggatgca aagacagtcc gtgggaatct cggtgatctc ctcatgcaga atacgaatgt
      781 tcggatgccc ttctagtgcc tgagtaatct tgagtgaaaa ctgctcgcga tcgacggcca
      841 gggccgatcc agctggaacc cgtacttcat ctgccacctg aataatgagt gagttgaggc
      901 ggcgcatttc ttcctttaag atacctgggg cattcaggag atctgcagag ccgagcgaat
      961 tcgaacagac caactctgcc aaaccaccgg tcttgtgtgc ctgtgtcatc tctttcgggc
     1021 gcatttcata gagcgtcact ttcgctccgc gattggccgc ctgccacgcc gcctcagatc
     1081 cggccagtcc acccccgata atgacaatat cttcgcgcat acgagcaaat ccccgagaag
     1141 acaaaccgag agcggtggca ttgtaggaac cgtgttttgg ggatgtcaag gcgagtgcgc
     1201 gacgcatcac gcatagcgtg accgttgatc caatttatga ccccgtgcta gacttcgcgt
     1261 catgtaggcg attagctcag gggtagagcg cttccttcac acggaagagg ccactggttc
     1321 gataccagta tcgcctacca ccttctccac ctctcccttc cttatctatg ttggctcgcc
     1381 caaattggcg gttttcgtga tgctgcctga actgctccaa tcctggacag aattctagag
     1441 gtcggctaca cttcagtcta tcgagagacc ggcgattctg aaccgtccaa gacatgtgag
     1501 gtcatctatg atgaggacta cagctcttgc cttgggaatt ctcctcctgg ccactgcggt
     1561 cggttgtgct gaaaaccgtc agcaacaggc tttggccgat gctgatacag gctatacccc
     1621 cccggtcagc atttacagtg tgatggaccc cacatcattg atctatactg atgtggcggc
     1681 cggagcttcc gcaaacgata acccctggcg ttgggccggt tttttcttgc acccggtcgg
     1741 ggtcgcgata gattacggcg tcaatcgctc gttttataca atctccagca agatgccata
     1801 ccttttcggg tatacggctg aggattcgat gttggatagc caacggcgct aactcttcat
     1861 agagatcgtg ttcagctaca acgcccgctt tccttgcgag agcgggcgtt gctgtctcac
     1921 ctcattggca aaccatttcg cgtttcatgt atgcttcttt cgtgagtcag aatccatgtg
     1981 acacgcaagc gcctcgtatt ctgccatttc gactgtgggt ggcctgctgg ctaggactga
     2041 gtctcttgct caacgcctgt ggaactccag ttcaaaagtc aaacctccca ccgtctgaaa
     2101 ccgatctggt cctcctaaaa tccccgaccc tctgtatgtc gaagtccgac ttcctcaaga
     2161 cacatcctgc ctctactctg aaaccgcaaa actggggcac cggacaagag ttggttctcc
     2221 ctgccgaccg aagcccgtca cagggcgatg aatcctactt ttttgatgaa gatggactgc
     2281 tagttggaac cgtctttacc tttccgtccg ggcttgactt gaatccctac ccagtccttc
     2341 gccacacctt gacgctgctc aagcccacat tggagttcta tctcaacgtg gcacaactgt
     2401 cctcaatgac gagcatgaac tccagcgcct tgtacgaaac cggcgacgag aaaacgacga
     2461 cacagtatct ggtcctgggg acgcgcgagc agccggtctt gctccaggca tctatgacga
     2521 tcgaccccta tgtacggctg ttctccccct atcgacgcga gttcttggaa cggttacgct
     2581 atccaagtgg ccggaagcca gggcaacagc tagacagtca aggcgcggag gacaaagaac
     2641 actttccctc actccagcaa tttgcacgcg gccaaaccgc acagctctct tactgcgggc
     2701 cccagaacta cgagatcgca tcttctgcct atcagaaagc gctcgcaagc ggctttacaa
     2761 acaaggtgtg gcaggcagaa gctcaccaca aactgggcct tgcgtgggaa gggatgggac
     2821 aatacgagaa ggccaagacc gagatgcttc agtcgctgac catcagaccc aacactcc
//
LOCUS       DATIPF010000195         4938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_920894, whole genome shotgun sequence.
ACCESSION   DATIPF010000195 DATIPF010000000
VERSION     DATIPF010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920894"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VLL06_04965"
     CDS             <1..248
                     /locus_tag="VLL06_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="archease"
                     /protein_id="HSS30349.1"
                     /translation="LLFDWLSEVVYWKDAAGVVFREAPLTLTEDGEVWLLRARLIGAP
                     VDQQTQELHADVKGVTKHLYELKQTGGRWKVCVVLDV"
     gene            291..1742
                     /locus_tag="VLL06_04970"
     CDS             291..1742
                     /locus_tag="VLL06_04970"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HSS30350.1"
                     /translation="MNLNTDMTVNRITDELWEIPMSEKPGMLVPARIYATQGILQSMD
                     RGVFDQVTNVACLPGIVRYALCMPDGHWGYGFPIGGVAAFDVEQGVISPGGVGYDINC
                     GMRLIRTNLTLADVKPRLERLMTELFRKVPAGVGASGFVRLSREDFGRVMTHGARWCV
                     ERGYGWHRDLACMEEGGCIEGADPSAVTDHAIQRGINQLGTLGSGNHYLEVQVVSNGR
                     IFDPVTAAALGITGHEQIVVMVHCGSRGFGHQVASDYLKVFEKVMRRHGITVKDQQLA
                     CAPFRSPEGQDYFSAMNCAANTAFANRQVITHQIREAFATIFGQSAEALGMELLYDVA
                     HNIAKIERYAEGELVVHRKGATRAFGPGSPELPDTFRQTGQPVICGGSMETGSYLLVG
                     TDQAVRDTFGSTMHGSGRTMSRSQAKKTVRGEQLQRQMKQRGILVKAVSMSGLAEEAG
                     LAYKNISEVVESVDRAGITKKVVELRPIGNIKG"
     gene            1767..2129
                     /locus_tag="VLL06_04975"
     CDS             1767..2129
                     /locus_tag="VLL06_04975"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS30351.1"
                     /translation="MMEQRKNLRFHVKFRSSFSSIGIVGGEGTLVDLSIRGCRIESPT
                     DVQPGASLEVRIAAIEHEPPIKIQQALVRWIRGRQFGIEFVTMVPEEWARLQHTVKQI
                     EMEPYERDLEAAKRQESS"
     gene            2355..3071
                     /locus_tag="VLL06_04980"
     CDS             2355..3071
                     /locus_tag="VLL06_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSS30352.1"
                     /translation="MQDEDDRAEGRTYIVGDRDSDKAIIIDAVIENIDRDLKILDELD
                     LTLIFAVETHIHADHVTGASILKDRTGAQIVFGGGAQGMVTGADMFLQEGQELHIGNT
                     TMKALATPGHTDSCTSYLLPGAVFTGDTLFIRGNGRTDFQGGSADKLFDSVRKKLFVL
                     PDDTIVYPGHDYNGRVSSTIGEEKKFNECLKLSIDKDTFVEMMHKRNKPRPPKMDIAV
                     PANLRAGRMAKNEIGINQRH"
     gene            3297..3740
                     /locus_tag="VLL06_04985"
     CDS             3297..3740
                     /locus_tag="VLL06_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30353.1"
                     /translation="MKNHARRLWGAVGIGLLNIFVVVSVGASDKPVTGDKVIQETQEA
                     VTATKDYTIQQKDAFQRKVQTELDEMQGRIAQLRGQVKHASAEARADIQKAISDLEKK
                     KELAEKKAQEIHSATASSWEQVKAKTSAAMDDLRESFNRAKSHLP"
     gene            3751..4641
                     /locus_tag="VLL06_04990"
     CDS             3751..4641
                     /locus_tag="VLL06_04990"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HSS30354.1"
                     /translation="MKYALYPGCAAQGATPELYQSTRAIIGQLGIEVVELTSAACCGA
                     GVVTEAHPDMALVFNARTFAQAEVLGLDVMTICGTCQGVMSAANRQLKTEPATLNRIN
                     ALLAPDGLTYHGRVQVKHLLWIVVRDIGLARLSSLVSMPMRDFRIAPFYGCYILRPSW
                     DLGFDDPENPQSLERVITAVGGEPVAYAGRTKCCGFPIILEKEAIAVAMAGMHMNEAK
                     EKGADFMVTPCPLCHMSLDIYQDRAGRAVNRELHLPILHVPQLLGLAMGIPAKDLGLS
                     HHVISVDSILATREHRRTHP"
     gene            4663..>4938
                     /locus_tag="VLL06_04995"
     CDS             4663..>4938
                     /locus_tag="VLL06_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSS30355.1"
                     /translation="MNVVNLSDYQLFSHEKMKKNNMFQTPRFFCDIYCFEPGQEQKGH
                     IHGEQDKVYLVLEGRGTFQVGSETQMLGAGQGTLAAAGEEHGVKNHTD"
BASE COUNT         1128 a         1266 c         1495 g         1049 t
ORIGIN      
        1 cgctgttgtt cgattggttg tcggaagtgg tctactggaa ggacgccgcc ggcgtggtct
       61 tccgggaggc gccgctcact ctgaccgagg atggggaggt ctggctgttg cgggcacgtc
      121 tgatcggcgc gccagtggac cagcagacac aggagctcca tgccgatgtg aagggggtca
      181 cgaagcacct ctacgagctc aagcagacag gcggtcgctg gaaggtttgt gtggtactcg
      241 atgtgtgatg aaacatgatt accggttaga ctcaacctgc catctttgac atgaacctca
      301 acaccgacat gacggtcaat cgcattacgg atgaactgtg ggaaatcccc atgtccgaaa
      361 aacctggcat gcttgtgccg gcccgcatct atgcgacgca agggattttg caatcgatgg
      421 accggggagt gtttgaccaa gtcacgaacg tcgcctgttt gcccggtatc gtgcgttatg
      481 ccctctgcat gccggatggc cattggggtt atggatttcc gatcggtggt gttgcggcgt
      541 tcgatgttga acagggcgtc atttcacctg gcggcgtggg ctacgatatt aactgtggca
      601 tgcggttgat tcgaacaaac ttgacgctgg cggacgtgaa gccccgcttg gaacggctca
      661 tgaccgagct ctttcgaaaa gtgcctgctg gtgtcggggc aagcgggttc gttcggctct
      721 cgcgggaaga ttttggtcgc gtgatgaccc atggggcaag gtggtgcgtg gagcggggct
      781 atggatggca tcgggatctc gcctgcatgg aagaaggcgg ctgcattgaa ggggccgatc
      841 cgtcggccgt gactgaccat gccatccaac gcggcatcaa ccagctgggg accttgggat
      901 cgggcaatca ctatctagaa gtccaggtgg tctcaaacgg acggatattc gatccggtca
      961 cggctgcggc ccttggcatt accggccacg aacagatcgt cgtcatggtg cattgtggtt
     1021 cgcgcgggtt cggccatcag gttgcgagcg actatctcaa ggtgttcgaa aaggtcatgc
     1081 gccgtcatgg catcaccgtg aaggatcagc agttggcctg cgctccgttc cgatctcccg
     1141 agggacagga ctacttttcc gccatgaatt gtgctgcgaa caccgccttt gccaatcgcc
     1201 aagtgattac gcaccagatt cgcgaggcct ttgcgacgat cttcggtcag tcggccgagg
     1261 cattggggat ggagctgttg tatgacgtgg cgcataacat cgccaagatc gaacggtatg
     1321 cagaagggga gttggtcgtg catcgcaaag gagcgaccag ggcgtttggg ccggggagcc
     1381 cggaactacc agacacgttt cgacagactg gccagccggt catctgcggc ggttcgatgg
     1441 agaccggctc gtatctgctg gtgggaacgg atcaagcggt gcgcgacacg tttgggtcga
     1501 cgatgcacgg gtctgggagg accatgtccc gctcgcaagc gaagaaaacg gttcgcggcg
     1561 agcagctgca gcggcagatg aaacaacgcg gtattctggt caaagccgtg tcgatgtcgg
     1621 gcttggcgga agaagccggc ctggcctaca aaaacatttc cgaagttgtt gagtccgtcg
     1681 atcgtgcggg aatcacaaaa aaggtggtgg aattgcgtcc gatcgggaat ataaaggggt
     1741 agccctacct tcccatgcga ggtgcgatga tggagcagcg aaagaatctc cgctttcacg
     1801 tcaaatttcg cagttcgttt tcctccatcg gcatagtcgg cggagagggc actctcgttg
     1861 atctctccat caggggttgt cgcatcgaaa gtcccaccga cgttcagccg ggagcctctc
     1921 tggaggttcg gatcgcggcg atagagcacg aacctccgat taagattcag caagcactag
     1981 tccggtggat ccgcgggcgg caattcggca ttgagtttgt gacaatggtc ccggaagagt
     2041 gggctcgtct ccagcacacg gtaaagcaga ttgaaatgga gccctacgaa cgggatctag
     2101 aggcggctaa gcgtcaggaa tcttcttaaa cgtgtcggat gtaggccagg ctgatcaatc
     2161 cgactcttgg ggtgcgtgac ggccctcctt cttccgaact tcatccggtt atagaccagg
     2221 ccgccatgaa aaagtaggat aaatcattgc tgtgtgcgtg ccctaagggt gtagaaaact
     2281 cggaaatctt cggagtgccg gctatggcaa tcagagacac ctataccgtc acggtcctct
     2341 tggacgtgaa tgggatgcag gacgaagacg acagggcgga aggccgcacc tatatcgtgg
     2401 gcgaccgcga ctccgacaag gcgatcatca ttgatgcggt tatcgaaaat atcgatcgag
     2461 acctcaaaat acttgatgag ctggatctga cgctcatctt tgccgtcgaa acgcacatcc
     2521 atgccgacca tgttaccggc gcctcaatcc tcaaagatcg tacaggggca caaatcgttt
     2581 ttggtggcgg tgcacagggt atggtgacgg gtgcggatat gttcctacaa gagggccagg
     2641 agttgcacat cggcaacacg acgatgaaag cgcttgccac cccgggccac acggacagct
     2701 gtaccagcta tctcctgccc ggcgcagtgt tcacaggcga taccctgttc atacgcggaa
     2761 atggccgtac ggattttcaa ggcggttccg cggacaaact gttcgactcc gtgcgcaaaa
     2821 aactcttcgt gctgcccgac gataccatcg tctatcctgg tcatgactac aacggcaggg
     2881 tgtcatccac catcggtgag gagaagaaat tcaacgaatg tctaaaactg tcgatcgaca
     2941 aagatacgtt cgtcgagatg atgcataaga gaaataagcc gcggcctccg aagatggata
     3001 tcgccgtgcc cgccaatttg agagcgggcc gcatggccaa aaatgagata ggaatcaacc
     3061 agaggcatta atggatcttg caggtgggcg acactccgaa gaaataatat ggtgagccag
     3121 ttcgcagcgc atgacgggct gtcttcctcc cccttcctca tctgatacag gcacagctcc
     3181 catgtatccc cgcatgctcg tgcttcacgc gtcatggtcc gtgcacggtg actttccgac
     3241 atgcggaagt tatactccaa cacgagatgt acatcggtgg aatggagggg attgctatga
     3301 aaaatcatgc cagaaggttg tggggtgctg tgggaatcgg cttgctcaac attttcgttg
     3361 ttgtctcagt tggcgcgtcg gataagccgg tgacgggtga caaagttatt caagaaacgc
     3421 aggaagcggt gacggccact aaggactata cgattcagca gaaggatgca tttcagcgaa
     3481 aggtccaaac ggaactggac gagatgcaag ggcgcatcgc gcagctccgt ggacaagtca
     3541 agcacgcatc ggcggaagca cgagcggaca ttcagaaggc catcagtgat ttagaaaaga
     3601 aaaaggagct ggccgaaaaa aaagcacagg agattcattc cgcaacggcc tcttcctggg
     3661 agcaggtgaa ggcgaaaacg tcggctgcga tggacgatct tcgagagtct ttcaatcggg
     3721 ccaagtctca tctcccgtag cggtacaccc atgaaatatg ccctctaccc aggctgcgcg
     3781 gcgcaagggg cgacaccgga actttatcaa tcgacgaggg cgatcatcgg ccaattgggg
     3841 atcgaggtcg tcgaactgac gtcggccgca tgttgcggcg ccggtgtggt gacagaagca
     3901 catcccgaca tggcgctggt attcaatgcc agaacctttg cgcaggcgga ggtgctcggt
     3961 ctggacgtca tgacgatctg tggcacgtgc caaggggtca tgagtgctgc caatcgccaa
     4021 ttgaagacag agccggcgac cctgaaccgt atcaacgctc tcctcgcacc ggacggtttg
     4081 acctaccacg gtcgtgtcca agtcaaacat ctcttgtgga ttgtagtccg agacattggg
     4141 ctggctcgcc tgagctccct ggtctccatg ccgatgcggg acttccgcat cgctccgttt
     4201 tacggttgct atatcctacg cccgtcgtgg gatcttggat ttgacgatcc tgagaatcct
     4261 caatcccttg agcgcgtcat cacagccgtc gggggcgaac cggtggccta tgccggaaga
     4321 accaaatgct gtgggtttcc catcatcctt gaaaaggaag ccattgccgt ggcgatggca
     4381 gggatgcaca tgaacgaagc caaggagaag ggagccgatt ttatggtgac accttgtcct
     4441 ctctgtcaca tgagtttgga tatttatcaa gatcgagctg gtcgtgcggt gaaccgcgag
     4501 ctccacttac cgatcttgca cgtcccgcag ttgctcggtc tagcaatggg aattccggcc
     4561 aaggacttgg ggctctcgca tcacgtgatc tcggtcgatt ccattctcgc cacgcgtgaa
     4621 catcgtcgaa cgcatccgta acacctcgaa aggagttgat gaatgaacgt ggtcaatctc
     4681 tctgattatc agctatttag ccatgagaag atgaagaaga ataatatgtt tcagacgcca
     4741 cgattcttct gcgacatcta ctgctttgag cctgggcagg aacagaaggg gcacatccac
     4801 ggcgagcagg ataaagtcta tctcgtactt gaggggcggg gcacgtttca ggtcggttca
     4861 gagacacaga tgcttggtgc cgggcaggga accttggcgg cggccggtga agagcatggg
     4921 gtgaaaaacc acacagac
//
LOCUS       DATIPF010000196         6999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1009995, whole genome shotgun sequence.
ACCESSION   DATIPF010000196 DATIPF010000000
VERSION     DATIPF010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009995"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..903
                     /locus_tag="VLL06_05000"
     CDS             157..903
                     /locus_tag="VLL06_05000"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HSS30356.1"
                     /translation="MSTFFVIPDAVTPPTIRITGDLLHHLRDSLRLHPGDRLTLNDGH
                     GTRYRVEVTHVTPQAIDNRIIDQQTEPTRRTSPIVLGQALIKGDKMDWVIQKATELGV
                     ATIVPIHSTHSVIKPNPERLEHQRARWTRIARDAAQQSERWTLPVIADPVDLAEICLH
                     YASAPLKGMLAERSSGPSLATISLPQDRQHPIIVLIGPEGGWAPDEQRLAQEQGFLPL
                     TLGPRILRAETATIAALSILQSRLDEQGKD"
     gene            complement(1130..2131)
                     /locus_tag="VLL06_05005"
     CDS             complement(1130..2131)
                     /locus_tag="VLL06_05005"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HSS30357.1"
                     /translation="MRNGLPESWLDSFLTSGGCLVVVVLATLLCASPSWTADAPLTIT
                     PQVSHTEAILMGLYFHSPQLGWAVGSGGTILKTVDGGKKWKKVVSGTTASLSAVFFAD
                     EKQGWVVGANGTIRRSQDGGTTWHAQLLDTQVPLYGLSFVSPNEGWVVGGNGTILHTK
                     DSGSHWFDQSSKTSAALYGIQFLTAQHGTVVGAVGTVLMTDDGGATWIPQETQGAVTL
                     FDVYFSDTSTGWTVGNAGAIFHTTDGGNHWIDRTLPCTSTCTKLTDLLRVRFTGLQTG
                     WIVGERGVVYRTHDGGYTWTEQGTIAKVSLFGLSFPIGTEGWASGEKGTIIHLAVGR"
     gene            complement(2128..3810)
                     /locus_tag="VLL06_05010"
     CDS             complement(2128..3810)
                     /locus_tag="VLL06_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HSS30358.1"
                     /translation="MNACVAGVWTMTLWAGLSAGVSAATPSVLELHPSTAVPGATLSI
                     GGKGFGTFRSVQVNRVTVGGFPALIQRWESDLIEVKVPSQAQSGPVQVIAGKKKMTAG
                     MLTVVRPTITAVTPEEIELGHTVQVAGVHFGVTAGARDPNTMFGVNDVMIGNVVVRPR
                     RWKDDLIEVEVPTNAASGDLVVRLASSDPLPDGSCCAPVQYTVSNAVPLKLIPSIRVD
                     PVSGPVGTKVVLFGTGFGETKTAGDKVTFGGHLATLAQWSDNTIVVHLPMDAETGPIV
                     LTLQGRERTVGTFTVHVPKVIAMTPPAAPIGTLLRISGEHFGFYSESGATPYAFTDFN
                     TGENRVDIGGIRAVIYRWQDDRIDVWVPFSAKSGPVVIYRGATKPNSDGSCCATKETL
                     QTEAGVFTLVTPKIDSYSPHSGGLDELITIKGSGFGSFLKTAEHADLSLNQGAYKRRD
                     DVALGENVSRTEVLFSNLAAQVMSWTDTEIVVRVPHRNLYGVGKRHEFVDELATGQLI
                     VRRGSWDVQPDNTCCTSKKWLTLDVGTFTIVAKGMPDSGFFNNNRPDADTAQ"
     gene            complement(4073..4543)
                     /locus_tag="VLL06_05015"
     CDS             complement(4073..4543)
                     /locus_tag="VLL06_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSS30359.1"
                     /translation="MSLRTSYEFRLTELHIELPLPPQPLATYVPAVQAGGLLFLSGVL
                     PMRDGQLAFSGKLGRDLTVEQGMESARLALLNALAIARQELGTLDRITRVVKLVGHVA
                     SAEGFVQQPQVLNGASGLLVQIFGESGRHARVAVGAAELPRGAPVEIEVIFSVS"
     gene            complement(4547..5704)
                     /locus_tag="VLL06_05020"
     CDS             complement(4547..5704)
                     /locus_tag="VLL06_05020"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HSS30360.1"
                     /translation="MINRVIVLVIDGLGVGALPDAAEYGDAESHTLSHLADAVGGLSL
                     PTLETLGLGHITDIKGVRTMWQPQGCFGRLGFLSKGVDSMVGYWEMAGHLTDAAGPLY
                     PNGFPPDMIAVIEQAFGRKVIGNRRASGTVMLRECEAEHLSSGALIVWTDGRRTCHVA
                     LHENVMRRDELFQRCRDVRKLLKDPWGIWRVSAHPLLGEAGGVQFSPQPRQFVLEPPG
                     PTIFDVLNRASQILVGVGKVSDLFSGRGLTSSLPVGPWTVLLTDVTKMLRKVPRGLIF
                     VGLDVLESDAAQSASALQDFDRRLPGLLEQLRPGDLLVLTGNHGRDMTKADQVPTREY
                     VPLLTTGPKLAQGVHLGIRASAADLGHTIVEALQGDQFPVGESFLDALRAG"
     gene            complement(5753..6424)
                     /gene="deoC"
                     /locus_tag="VLL06_05025"
     CDS             complement(5753..6424)
                     /gene="deoC"
                     /locus_tag="VLL06_05025"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HSS30361.1"
                     /translation="MSEWNLPDLIDHTVLRPEATKFDVLRLCQEAKEHRFTVIFVPPC
                     YVDDAVAAVAGTGIRIGIPIGFPLGGHTTKTKVAEALEAVARGAQVLDMVINVSQLKS
                     GDHEYVRRDIAEVVHATPGVEHKVILETCYLTPQEKRTACQLVVEAGADYVKTSTGFG
                     AGGATVEDVRLMKEAVAGRVKVKASGGIRDWKTTLAMLEAGADRIGTSASLVIVAEWQ
                     RQTTS"
     gene            complement(6676..>6999)
                     /locus_tag="VLL06_05030"
     CDS             complement(6676..>6999)
                     /locus_tag="VLL06_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HSS30362.1"
                     /translation="LPAFDGNLHKKDLSSPSPYNTYRVQGLPPGPIANPGIQAIRATL
                     YPSDSRSLYFVSRNDGTHQFSATLIEHNKAVEKYQKRPFRRGTQSQTSRSSGERVLAY
                     AEGGS"
BASE COUNT         1601 a         2182 c         1831 g         1385 t
ORIGIN      
        1 ctcctacaaa actgaatgct cgtcagaaag agttgctcaa agaatttgcc aaagagagcg
       61 gcatgtcgat tgaagaagac ggcgacggtt tcttcgacaa gatgaagacc ttcttcgaat
      121 aacaccgacg gtcatcgtcc agatatcccc cgcatcatgt ctacgttctt cgtcatccct
      181 gacgccgtca ctccccccac gatccgaata accggtgatc ttcttcatca tcttcgcgat
      241 agtcttcgcc tgcacccggg ggaccgcctc accctgaacg acggacatgg aacccgctat
      301 cgtgtggaag tgacccatgt caccccacag gccatcgata accgtatcat cgaccagcag
      361 accgagccga cgcgcaggac ctcacccatc gtgctcggac aggcgttgat caaaggtgac
      421 aagatggact gggtcattca gaaggcgaca gaacttggag tggcaaccat cgtaccgatt
      481 cacagcacac acagcgtgat caaacccaac ccggaacgtc tcgaacatca acgcgccaga
      541 tggactcgca ttgcgcggga cgcggcacaa cagtcggaac ggtggaccct tcctgtcatc
      601 gcagacccag tagacctcgc agagatctgc ctgcattatg cctcagcacc tttgaagggc
      661 atgctcgcgg agcgctccag cggcccttcg ctcgcaacca tatcgctgcc acaggaccgc
      721 cagcatccaa ttattgtatt gatagggccg gagggaggat gggcaccgga cgagcaacga
      781 cttgcacagg agcagggctt cctcccgctc accctcggac cacgaatctt gcgggccgag
      841 actgcaacga tcgccgcact cagcattctc caatcacgac tcgacgagca ggggaaagac
      901 tagaaaactg taggatgctc aaaaagaccg cccagcaagg ccgcagccga tgaaggcacc
      961 ggaggcgtac ctttcgtacc tgcccgcccc gaactgctaa cgcaactcta tcccgatgga
     1021 ctaccttgag gatgctttcg aggcgagaac gacgcttggt gaaaggcgcg tcccggcgcg
     1081 ccagggtggg cgggtgagaa cagaggattt ttcagcatcc tactagccgt cagcggccaa
     1141 cggccagatg aatgatcgtg cccttttctc cgctagccca accctctgtt cctatcggga
     1201 atgagaggcc gaacagcgag accttcgcga tcgtaccctg ctctgtccag gtgtagccgc
     1261 catcgtgagt ccgatagacg acaccccgtt cccctacgat ccagccggtc tgaaggccgg
     1321 taaaccgcac tctcaagagg tcggtcagtt tcgtacaggt gctggtacag ggcaaggtgc
     1381 gatcgatcca gtgattcccg ccatcagtcg tgtggaagat ggcaccggca ttccccacag
     1441 tccacccggt cgacgtatct gaaaaataca catcgaacaa tgtgaccgct ccctgtgtct
     1501 cttgcggaat ccacgtcgcc ccgccgtcgt ctgtcattaa gacggttccc acagccccaa
     1561 ccaccgtccc atgctgcgcg gtgagaaact gaatgccgta gagcgcggca ctcgtcttac
     1621 tcgattgatc aaaccaatga gacccgctgt ccttcgtgtg aagaatggtt ccgttgccac
     1681 caaccaccca tccttcattg ggcgacacga atgagaggcc atacagcggc acctgggtat
     1741 cgaggagttg tgcgtgccag gtcgttcccc cgtcttggct ccggcgaatc gtcccattag
     1801 ctccgaccac ccacccctgc ttttcgtcgg caaagaagac agcggagagg cttgccgttg
     1861 tgccgctgac aacctttttc catttctttc cgccatcgac cgtcttcaaa atggtaccgc
     1921 cagacccaac ggcccagcct agctgaggcg aatgaaaata gagccccatc aggatggctt
     1981 ccgtatgact gacttgaggg gtgatcgtca gaggggcgtc agcggtccac gaaggcgatg
     2041 cacacaacaa tgtcgccaac accacgacca ccagacatcc gccactggtg agaaaggaat
     2101 ccagccatga ttcgggtaat ccgtttctca ctgggcggtg tcggcatcag ggcgattgtt
     2161 attgaaaaat ccagaatccg gcatgccctt ggctacaatg gtgaaggtgc ccacatccag
     2221 tgtcagccac ttcttcgagg tgcaacaggt gttgtcgggt tgcacatccc aggagccccg
     2281 ccgaacaatc aactgaccag tggcgagctc atcgacgaac tcatggcgct tccccacacc
     2341 atagagattg cgatgaggca cccgcacgac aatctcggta tccgtccagg acatcacctg
     2401 cgccgcgagg ttgctgaaca gcacttccgt gcgcgagacg ttctcgccca acgcgacatc
     2461 gtctcgtcgt ttgtaggccc cctgattgag gctcaaatcg gcatgctcgg ctgttttcaa
     2521 gaatgagccg aaccccgacc ctttgatggt aatcagctca tcgagtccgc ctgagtgagg
     2581 gctgtaggaa tcgatcttcg gcgtcacaag cgtaaagacc cccgcttcag tctgtaacgt
     2641 ctccttcgtc gcacaacagg agccgtcgga gttgggcttt gtcgcgcctc gatagatcac
     2701 gaccggcccg ctcttcgcac tgaagggcac ccatacatca atgcgatcgt cctgccagcg
     2761 atagatgacc gcgcgaatcc ctccgatatc cactcgattt tctcccgtat tgaaatcggt
     2821 aaaggcgtag ggggtcgctc cactttccga atagaatccg aaatgttctc cgctaattct
     2881 gaggagcgta ccgattgggg cggcaggagg agtcatggcg atcacttttg gcacgtgaac
     2941 cgtaaaggtc ccgaccgttc gctcacgtcc ctgaagagtc agcacgatgg gccctgtctc
     3001 cgcatccatc ggaaggtgca ccacgatggt gttatcggac cactgggcga gggtggcgag
     3061 atgtccaccg aacgtcacct tgtcaccggc agtcttcgtc tcgccaaaac ccgtaccgaa
     3121 caagaccact ttggtcccga ccggtccgct cactggatcc acgcgaatgc tcggaatcag
     3181 cttcagcgga acggcattgc tcaccgtgta ctggaccgga gcgcaacagg atccatccgg
     3241 taagggatcg gaggaggcca atcgaaccac caggtcgccc gacgcagcat tcgtgggaac
     3301 ctcgacttcg atgaggtcgt ctttccaacg gcgcggcctg acaaccacgt tcccgatcat
     3361 cacatcattg actccgaaca tcgtattggg atcgcgagca ccagctgtga caccgaaatg
     3421 tacgccggca acctgcaccg tatggcccag ttcaatctct tccggcgtca cggcggtaat
     3481 ggtcggccgt accactgtca acatcccggc agtcatcttc ttcttcccgg cgataacttg
     3541 aacgggaccg gattgtgcct gggaaggaac cttcacttcg atcaggtcgg attcccatcg
     3601 ctgaatcaac gccgggaacc caccgacggt cacgcgattg acctgcacgg atcgaaatgt
     3661 gccgaacccc ttccccccga tggacagagt ggcaccggga accgccgttg acggatgtag
     3721 ctcgagcacg gatggagtgg ccgctgacac gccggcgctc agtccggccc acaacgtcat
     3781 tgtccacacg ccggcgacac acgcattcat aaattggttc atgtagttgc tctgatctat
     3841 ctggattggc tggtgtcttt tgcccttcga accaaaccgg ccactggcac aagacaaacc
     3901 tgatagaccc cacaatggtc ttctgatgcg taccgatttt tcagcattct gttagaaaga
     3961 gttcaccgta gatgaagcaa cggagcaatc gaactatagc aagcagtttt ttggagagtc
     4021 aagccgacga gcctgtacta gtcacgcgtt cctggccagt tcggaccagc gattacgaga
     4081 cggagaatat tacttcaatt tcgacgggag ccccgcgagg caactccgca gcccccaccg
     4141 ccacacgtgc atggcgaccg gattctccaa agatctgaac cagtaggcca gaagccccgt
     4201 tgagcacttg gggctgctgc acaaaacctt ccgccgacgc gacatgcccg acgagcttta
     4261 cgacgcgcgt gattcggtcc aatgtcccga gttcctgccg ggcaatcgca agtgcattca
     4321 gaagcgcgag tctggccgat tccatcccct gctcgaccgt cagatcacgt ccaagcttac
     4381 cggagaatgc caactgtccg tctcgcattg ggaggacgcc ggacaaaaac aagagacctc
     4441 cggcctggac agccggaacg tacgtcgcta acggttgcgg cggcagcggc aactcgatat
     4501 gcagttctgt gagccggaat tcatacgacg tgcgaagcga catccatcac cctgcgcgga
     4561 gtgcatcgag aaaactctca cccacgggaa attgatcccc ttgcagggct tctacaatgg
     4621 tatgtccaag atcggcagcc gacgccctga tgccgagatg aacgccctgc gccaactttg
     4681 gcccggtggt caagagcgga acatactccc tggtcgggac ctgatccgcc ttcgtcatat
     4741 cccggccatg attccctgtc aggacaagca ggtcgcctgg acgaagttgc tccagcaacc
     4801 cgggaagccg tcgatcaaaa tcttgaagag ccgaggcgga ctgtgccgca tccgattcca
     4861 ggacatcaag cccgacaaaa atcagtccgc gtggaacctt tctcaacatt ttcgtgacgt
     4921 cggtcaaaag caccgtccac ggtccaaccg gaagggaact cgtgagcccg cgaccgctga
     4981 agagatcgct gacttttccc accccgacca ggatctgact cgcgcgattc agcacatcga
     5041 atatggtcgg ccccggaggt tcaagcacaa attgacgggg ttgcggactg aactgtaccc
     5101 caccggcctc acccaagaga ggatgggctg acacacgcca aattccccaa gggtccttga
     5161 gtaattttct cacgtcccgg cagcgctgaa ataattcgtc acgacgcatc acattctcat
     5221 gcaacgccac atgacaggtt cgtcgtccat ccgtccagac gatcagggca cccgacgaca
     5281 gatgttcggc ctcgcactcg cgcaacatga ccgttcctga ggcccggcga ttgccgatca
     5341 ccttgcgccc aaacgcctgc tcgatgaccg cgatcatgtc aggaggaaat ccgttcggat
     5401 atagcggccc agccgcgtcc gtgagatgac ccgccatttc ccaatagccg accatggaat
     5461 ccacaccctt cgaaagaaat cctagacgac cgaagcaccc ttgcggctgc cacatcgtcc
     5521 gcacaccttt aatgtccgtg atgtggccaa gcccgagcgt ttccaacgtg ggaaggctga
     5581 gtcccccaac cgcatcggcc aaatgagaca gggtatggga ttcggcatcg ccgtattctg
     5641 ccgcatcggg cagagccccg acgccgagtc catcgatcac gagtacaatg acacgattaa
     5701 tcatggtcgc actataccag aagagtcact catgggatat ggagtgacgt tgctatgaag
     5761 tggtttgtcg ttgccactcg gccacgatga caagacttgc gctggtgccg atcctatccg
     5821 caccggcttc tagcatggcg agcgtggtct tccagtctct gatgcctccc gaggccttca
     5881 cctttacccg acctgccaca gcttctttca tcagcctcac atcctcaacc gtcgcaccac
     5941 cggcgccgaa cccggtcgac gtcttcacgt agtccgctcc cgcctccacg accaactgac
     6001 aggcggtacg tttttcctgc ggcgtcaaat aacaggtttc gagtatgacc ttgtgttcaa
     6061 cacctggtgt cgcatgcacg acttcagcaa tgtcccttcg cacatactca tgatctcccg
     6121 acttcaactg gctcacattg atgaccatat ccagcacctg cgcgccgcgc gcgaccgctt
     6181 caagagcttc ggcgactttg gtcttcgtcg tgtgaccgcc caggggaaac ccgataggga
     6241 taccgatgcg aatccccgtg cccgcgacag cggccaccgc gtcatccaca taacaaggcg
     6301 gaacgaaaat cacggtgaat cgatgctcct tcgcttcctg gcacaatcgc aggacatcga
     6361 acttcgtcgc ctccggccgc agcaccgtat ggtctataag atccggcaaa ttccattcag
     6421 acataacagg ctcataaaaa actattgtgg aatggtgaaa attcaatggc cgcacatcaa
     6481 agacaacacg agaacccgcg caggatgctc aaaaattcca gacttctcac acgcccaacc
     6541 ctggcgcgcc aagacgcgcc ttgtccctgg caaggccgca ggcgagtcga aaccggaggc
     6601 gtaccctctg gggtacgttg aggatttcga tgagccgaga acgaggctgg cggacttttt
     6661 cagcatcctg ataactcatg agcccccttc tgcgtaggca agtactctct ccccggagct
     6721 acgtgacgtc tgggactggg ttccacgacg aaagggccgc ttctgatact tctccacagc
     6781 cttgttgtgt tcgatcagcg tcgcagagaa ttgatgcgtg ccatcgttcc gggaaacaaa
     6841 atacaacgag cgtgaatcag acggatagag cgtcgcgcga atggcctgaa tgcctgggtt
     6901 ggcgatcggt ccaggcggca gtccctgtac ccgataggta ttgtagggac tggggctgga
     6961 caggtctttc ttgtgcaaat ttccatcgaa tgccggcag
//
LOCUS       DATIPF010000197         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_391275, whole genome shotgun sequence.
ACCESSION   DATIPF010000197 DATIPF010000000
VERSION     DATIPF010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391275"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            525..941
                     /locus_tag="VLL06_05035"
     CDS             525..941
                     /locus_tag="VLL06_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HSS30363.1"
                     /translation="MIGRSPSQKFLMIGTILAGSGVAAGAFGAHALKEILDAPMLQVF
                     ETATRYLMYHAFGLCIVSWAIDRYPEQGLQKSGWLFIIGILLFSGSLYGVSLAGIRWL
                     GAVTPVGGLTFIAGWLLLGWGVWRDTLPRTTASRTS"
     gene            complement(996..1970)
                     /locus_tag="VLL06_05040"
     CDS             complement(996..1970)
                     /locus_tag="VLL06_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30364.1"
                     /translation="MRLHIFILHTSSSRIGLALAMVVVVLFGTWLNAKADESLPSPVP
                     DFPSVIRAHADQLAKMESDKEARNLFVSAIGPALRLSEAARTLGAGPLPVKLAKELLV
                     PDLTEAVHRLMSDLSAWHLASTVRQAVSDQHITTVTEQLFMSPQARAWLDQHIQASWH
                     DSLHQLADVVVSPEWATNNQGESASTRPVAVLERAIRLEVDAMQASYREWDRIRNWNG
                     RVREIRGQARLCGTWQWIIHNHQKNHQEQKFSLLFPPAGQTQGTRPGLVETIVLGENV
                     YLRWEIDGRVQEDSLQFNKEGQRLEGTFVNSQGGWGSISGKKTADCTP"
BASE COUNT          441 a          548 c          602 g          487 t
ORIGIN      
        1 agagcttaca cccaccctct aagcgttcga gtacgcaccc gtccgcttga atatgactgc
       61 tgacgtgagc gagtacgcag tcaatcgtaa acttgggact atcccaccag gtctgttggt
      121 ttcttcgagc cagcatgata cgcggaggat agggtgggag aggggtgctt gcctattcct
      181 caagaggtgg ggggcttacc caaaaaggtg gtggagtggt cgctggtcct cctcctgttc
      241 agtcccgcgg cacgtcatgg gttatctgtc gaagaataaa gagcggggca tgagagaggc
      301 ctatcgagac ttagcccaca ttattcatca gtgcttctgc tacaatcgcc cgaaccgtca
      361 tgaacgatgt gggctcatgt ttctctggtc gaatccattg ctgatgtggt tggggagttc
      421 gcgtgcacgg gcgaaataga gtaaccagat aaacgacacg cagctcattt caaggcggga
      481 atgaatcgga tgaacaggac agatcacgaa ctaggaggat ggatatgata ggtcggtcac
      541 cttctcagaa attcctgatg attgggacga tacttgccgg atccggcgtc gctgccggag
      601 cctttggtgc ccatgcactc aaggaaattc tggatgcgcc gatgctccaa gtcttcgaga
      661 cggcaacacg ctacctgatg taccatgcgt ttggactctg tatcgtttcc tgggccattg
      721 atcgttaccc tgaacagggt cttcaaaagt ccgggtggct ctttatcatc gggattctgc
      781 tcttttcagg cagtctgtac ggagtgtccc ttgcggggat tcggtggttg ggggccgtga
      841 ccccggtcgg tgggctcacc tttatcgccg gctggctgct cttaggttgg ggagtctggc
      901 gagatacgct acctcgcacg acagcgagtc gcacatcata gccgtgaaga aggacatgca
      961 agagaggcac gcttcgggtg agtcgtacgt tcctgctagg gtgtacagtc ggccgttttc
     1021 ttcccgctga tcgatcccca tcctccttga gaattcacaa aggtgccttc gagacgttgc
     1081 ccttctttat tgaactgcag gctatcttct tggacccggc catcgatttc ccaccgtaga
     1141 tagacgtttt cgcccaacac aatcgtttca acgagtcccg gccgagtgcc ctgtgtctgt
     1201 cctgctggtg ggaagagtaa tgaaaacttc tgttcttgat ggtttttctg atgattgtga
     1261 atgatccact gccaggtccc gcagagccgg gcctgtccgc gaatctcacg gacccgacca
     1321 ttccagttcc gtatgcgatc ccactcccgg tagctggcct gcatcgcgtc aacttcgagg
     1381 cggatggctc gctccaagac cgctaccggt cgggtcgaag cagattctcc ctgattgttc
     1441 gtggcccatt cgggggagac aacgacatcg gcgagttgat gcagtgaatc atgccaggac
     1501 gcctgaatgt gctgatcgag ccatgccctg gcttgtggag acatgaacag ttgctcggtc
     1561 actgtcgtaa tgtgttgatc gctcacagcc tgcctgacag tggaggcgag gtgccaggcg
     1621 gacagatcgc tcatcaagcg atgaaccgcc tccgtcaggt ccggcacgag gagttctttg
     1681 gcgagtttga ccggcaacgg gccggccccc aaagtcctgg ccgcctcgct caaccgaagt
     1741 gctggcccga tggccgatac aaacaggttg cgggcctcct tgtcggactc catttttgcc
     1801 agttgatcag catgggcacg aatgaccgat ggaaaatccg gtacgggact tggaagggat
     1861 tcgtctgcct tggcattaag ccaagtcccg aataacacaa cgaccaccat cgcgagtgcg
     1921 agaccaattc gtgatgagga tgtgtgaagg atgaagatgt gaaggcgcat tgcactactc
     1981 taccgtgcct ccatccacac cctcaagcat ggactagcga cacaaagcta tggggaaact
     2041 ggttaggtgc cttgaaagga ggtcttgaga aatgtggc
//
LOCUS       DATIPF010000198         5793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_479365, whole genome shotgun sequence.
ACCESSION   DATIPF010000198 DATIPF010000000
VERSION     DATIPF010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479365"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /locus_tag="VLL06_05045"
     CDS             <1..1624
                     /locus_tag="VLL06_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HSS30365.1"
                     /translation="SGSSVHKLKEFAATMAIGLLIPPTGSSTAQSNSHTSAETGQPQH
                     HIHCRDFSAHLKDAVKNTGDKRTPSLPDADDPIESLPTTAVDDHAAGAPLIGIVVVSL
                     PQVPDTQEVASTVTVKAESLPTTNANASVTTSSGPSPTPTSNSATTPTGPPIQNIESA
                     SLLLQGLTVVVSSEDGTLGHEPVAAHPSPSKTTDSSSVSLPQRAFNPDSLNEVPTTPV
                     DSPTTNKPASRNGRGAPVHPAPIPLEPPSEASALSGTRPLGSLSPDQGAPLIMEKQGS
                     TNPLSTVQGQENALVQSPVRPPADIRPSAMIQEQGDHEATLLNQSQSVRVIQDSGGGE
                     QGSFDDLAQGQGEGSLFQFTAGGIPESVTRGHQSPLFIDQFMSARQTASLSEGAGSPV
                     VMSTTDHLKLTQSLLSGDRSATMTSTSGMPQTIYMQLPSHDSGPLNVRISMMDQTVHT
                     QFTTDRSDLGALLFMRQDQLQQNLIKSGLELGQFQVHVNQEGRQDALPDRQSRRNDGA
                     PEQQLASQDHNPQTQDRERPNHRPLRALSLFA"
     gene            1667..1948
                     /locus_tag="VLL06_05050"
     CDS             1667..1948
                     /locus_tag="VLL06_05050"
                     /inference="COORDINATES: protein motif:HMM:NF015896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HSS30366.1"
                     /translation="MIDIAAIVNSTNSPTPQTTGPKELGQDDFLKLLVTQLQYQDPLK
                     PLENQDFIAQTAQFSQLNQMTELVTLMKRSVALQEAATPPPAQQTQTQT"
     gene            2029..3255
                     /gene="flgE"
                     /locus_tag="VLL06_05055"
     CDS             2029..3255
                     /gene="flgE"
                     /locus_tag="VLL06_05055"
                     /inference="COORDINATES: protein motif:HMM:NF004238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HSS30367.1"
                     /translation="MGILTSLFSAVSGLNTYGNAMSVIGNNIANVGTVGFKSSRASFA
                     DLVSASLGGGSSAAQVGLGVFLNDVQTSFAQGSLSNTGNTLDLAIDGNGFFGLRDNTG
                     TTSYSRAGQFEVNNLGQIVDPSGRFLQGYQASATGVILGTVGNITLSTATVAPQATST
                     ASVVANLNSAATAPATAFSVTDATTYNFSNGITVYDTLGAQHQLRMFYVKTAVANTWN
                     LYSQIDGGAATAQTDLAFNTSGVLTGGGAQTFSLAMTGGAATPLSVAMDFTRLTQFGA
                     ASNLTDQTQNGFTSGSFQSLSVDSLGRVLAQFSNGQTRTLAQVVLNRFTNPNGLTRSG
                     ENGFAETVDSGAPLAGAPTNNGLGRLISQTIEQSNVDLGKEFVDMIITQRAFQANSRA
                     ITTTDEMLQELVNLKR"
     gene            3582..4124
                     /locus_tag="VLL06_05060"
     CDS             3582..4124
                     /locus_tag="VLL06_05060"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HSS30368.1"
                     /translation="MAETETAPPPPVPVAAGIPIKMVIVIVAGTLVLGLGGAFAFFKL
                     MGGGQEKGDQKAEATPAKVAGQGVKETKHDAPKVAIPGTIYDVEPFIVNLADTPEVRY
                     LKLTVKLELESQETSAELASRVPQLRDTILVLLTSKDAASIRTPQGKFLLRDEITQRV
                     NSLLPKPGVRAAYFTDFVVQ"
     gene            4144..5118
                     /gene="fliM"
                     /locus_tag="VLL06_05065"
     CDS             4144..5118
                     /gene="fliM"
                     /locus_tag="VLL06_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HSS30369.1"
                     /translation="MDKILSQDEIDALLKGVVSGEVETQAGDEGQSAGEVKQYDLFNQ
                     ERIVRGRMPTLELITDRFTRHQSMSWSALLNTKVEFNIIGTQIMKFGAFLKTVPIPSS
                     LNVFRMEPLRGNGLFVMNAFLVYLIVDYFYGGKAQTHVKPEGRDFTPIQLRIIKKLVQ
                     LAFTDMEKAWRAALSVKTELVRSEVNPQFAMVVSASEIVFVTTLQVQFGETTSDFFLV
                     YPYSMLEPIKEKLYSGIVSDQVEQDGSWATRFHEKLQECPLAVTVQLGTASIKVRDLL
                     DFAPGDLILLDQRPGDPLNCFVEGYLKFQGSPGVSKGNHACRVDKVIA"
     gene            5148..5528
                     /gene="fliN"
                     /locus_tag="VLL06_05070"
     CDS             5148..5528
                     /gene="fliN"
                     /locus_tag="VLL06_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HSS30370.1"
                     /translation="MANNEVANAGANAPTHTPSSSSSPAPASFPSLDGGGAVPAPKNI
                     DFILDIPMLVTVQVGSTKMVIRELLQLGQGSVIELEKLAGEPMEVLVNNKLVARGEVV
                     VVNEKFGIRLTDVISAAERVQQLA"
     gene            5600..>5793
                     /locus_tag="VLL06_05075"
     CDS             5600..>5793
                     /locus_tag="VLL06_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HSS30371.1"
                     /translation="MIDFWDSFIRMASALALVLALIGGLVLLVRRFGGARLSAPGSGQ
                     IVQVIGSGYIGPRKTISLVSV"
BASE COUNT         1315 a         1630 c         1601 g         1247 t
ORIGIN      
        1 atccggatct tctgtgcaca agttgaagga gttcgccgcg accatggcta ttgggcttct
       61 cattcccccg acaggttctt cgacggctca atcgaattca cacacgtcgg cagagacagg
      121 tcagcctcag catcatatcc attgcagaga cttttctgca catctcaaag acgcggtaaa
      181 gaatacagga gataagcgga ctccctcact tcccgacgcc gacgatccga tagagtcgct
      241 tccgacgaca gcagtcgatg accatgcagc gggagccccg ttgattggta tcgtggttgt
      301 gtcgctccct caagtgcccg acactcaaga ggtagcttcc acagttaccg tcaaggcgga
      361 gtccttaccg accacgaatg cgaacgcgag tgtgaccacg agttcgggcc caagcccgac
      421 tccgacctcg aactcggcca cgacccctac gggtcctccc atccaaaaca ttgaatcggc
      481 ttctctcctt ctacagggcc tgacggtggt ggtgtcttct gaggatggca ctctgggcca
      541 tgagcctgtg gcggcccatc cgtcaccgtc caagaccacg gattcttcta gcgtttccct
      601 gccgcagcga gctttcaatc ctgatagctt gaacgaggtc ccgacgacac cagtagactc
      661 accaaccacg aataagccag ccagtcggaa tggccgtggt gctccagttc atccggctcc
      721 catccctctt gaaccaccgt ccgaagcctc ggcgctttcc ggcacgcggc cactgggttc
      781 tctgagtccc gatcaagggg cacctcttat tatggagaag caagggagca ccaatcccct
      841 ttctacagtc cagggacagg aaaatgcact cgttcaatct cctgtccgac caccggcaga
      901 cattcgccca tcggcaatga tccaagagca gggggatcat gaggctacgc tactgaacca
      961 atctcagtcg gttcgtgtga ttcaagatag tggaggtgga gagcaaggct cattcgatga
     1021 tcttgcacag ggacagggag aaggaagcct gtttcaattc accgccggtg gaattccgga
     1081 gtcggtgaca cgaggccacc agtctccgct gtttatcgac caatttatgt cggcgcggca
     1141 gacagcatcg ttgtcagagg gggcgggctc ccccgttgtc atgtcgacga cagatcacct
     1201 caagctgaca cagagtcttc tcagcggaga tcgttcggcg accatgacat ccacgtcggg
     1261 aatgcctcag accatctata tgcaattgcc gtctcacgat tccggcccgc tcaacgtgcg
     1321 aatctccatg atggaccaga cggtgcatac acagttcacc accgatcgaa gcgaccttgg
     1381 tgctctgctg ttcatgcgac aggatcagct ccagcagaac ctgatcaagt ccgggcttga
     1441 acttggccag tttcaggtac acgtcaatca agagggtcgg caagatgcat tgcccgatcg
     1501 gcaatcccgg cgaaatgatg gagcaccgga gcagcagttg gcgtcacagg atcacaatcc
     1561 gcaaacccaa gatcgagaac ggcccaatca tcgtcctttg cgtgcgttga gcctgtttgc
     1621 ataacaagat gttcacggac tttattatta aggaaaggac agagtcatga tcgacatcgc
     1681 tgcgatagtc aattcgacga actcgcccac ccctcagacg accggaccaa aagaactcgg
     1741 ccaggatgac ttcttgaagc tcctcgtgac ccagctccag tatcaagacc cgctgaaacc
     1801 tctcgaaaac caggatttca ttgcgcagac ggcgcagttc agtcagctga atcaaatgac
     1861 cgagcttgtg acattgatga aacgaagtgt cgcattgcag gaagcagcca cacctccacc
     1921 ggcacagcaa actcagaccc agacgtaata ctgcagattg agtgggtagg atgatcatcg
     1981 tgcaccgaca ggcggacaat aaccggccat gaacaaagga gacacaacat gggaatttta
     2041 acctcgctct tttcggcagt cagcggactg aatacgtatg ggaatgcgat gtctgtgatc
     2101 gggaacaata tcgcgaacgt cgggaccgtc gggttcaagt cgagccgtgc atctttcgcc
     2161 gatctagtgt cggcctcgct cggcggcggg tcgagtgccg ctcaggttgg attgggtgtg
     2221 tttctgaacg acgtgcagac cagttttgct cagggttccc tttcgaatac ggggaacacc
     2281 ttggatctcg caattgatgg gaatgggttt tttgggttgc gcgacaatac agggacgacg
     2341 tcctattctc gcgcggggca attcgaagtg aacaaccttg gacagatcgt ggatccgagc
     2401 ggccgattcc tgcagggcta tcaagcctca gcgacgggag tcatcttggg aacagtcgga
     2461 aacattacat tgtcgacggc cacggtcgct ccacaggcca ccagcactgc gtcagtcgtg
     2521 gccaatctca attcagccgc caccgctccg gcgaccgcct ttagcgtgac cgatgccacg
     2581 acctacaact tttcaaatgg aattaccgta tacgacacgc tgggggcaca gcaccagctc
     2641 cgcatgttct atgtgaagac cgcggtggcc aatacgtgga acctctattc ccaaatcgat
     2701 ggtggtgctg caacggcaca aaccgactta gccttcaata cgagcggtgt gttgaccgga
     2761 ggcggtgcgc agacattctc attggctatg accggtgggg cggcgacgcc actgagtgtc
     2821 gcgatggatt ttacacggct tacgcaattt ggagctgcgt ccaatttgac cgatcaaaca
     2881 caaaatgggt ttacctccgg gtcgtttcag agtttgtcgg tcgattcgtt aggacgtgtc
     2941 ttggcgcagt tttccaacgg tcaaaccaga actctcgccc aggtcgtgct gaaccgattc
     3001 accaatccga atggactgac gcgttccggt gagaatgggt tcgccgaaac cgtcgattcc
     3061 ggtgcgccgc tggcaggggc tccgacgaac aatgggttgg ggcggctcat ttctcaaacc
     3121 attgagcagt ccaatgtgga tctcgggaaa gaatttgtcg acatgatcat tacacaacgg
     3181 gcgtttcaag cgaactcccg agccattacc acgaccgacg agatgctgca ggagctggtg
     3241 aacctcaagc ggtaagtcac tggaagtgga gatgaccgga gcgccgtgag tcggcgctcc
     3301 ggtcacccgt tcttttcttc gtctctcctc ccactcgatt cgatcgcagc aaccccaaga
     3361 gcctgacgaa cggcaatgtc ggtcaggctc ccacgcgtca gattattgac atcgtcaaga
     3421 ctctggctgg gctgccggtt gctttgctgg catagtcctt cgtattcaca cgccagatgc
     3481 cgtgtttcga tccatgcgtc acccgcctca tcctccatcc tgatggcata gccattgcaa
     3541 acttcccgtt cgtcgccacc gttcgactag aggagggttc tatggcagag acagaaaccg
     3601 caccaccgcc gccggtgcca gttgcggcag ggatcccaat aaagatggtc attgtcatcg
     3661 tggcagggac actcgtgctt ggcctcggag gagcgttcgc gttctttaag ctcatgggcg
     3721 gagggcagga aaagggcgac cagaaagctg aggcgacgcc tgcgaaggtc gcggggcagg
     3781 gagtaaaaga gacgaaacac gacgctccca aagtcgcgat cccgggtacg atctacgacg
     3841 tcgagccctt cattgtgaac ttggccgata cgccggaggt acgctatctg aaattgacgg
     3901 tgaagttgga gctcgagagt caggagacct cggccgaact cgccagtcgt gtcccgcagt
     3961 tacgtgacac gatcctggta ctcctgacca gtaaggatgc ggcgtcgatc cggactcctc
     4021 aaggcaagtt cctgttacgc gacgagatta cccaacgcgt caacagtctt cttcccaaac
     4081 ccggtgtgcg ggcagcctac ttcacggact tcgtcgtcca ataacgagga ccagcctgtc
     4141 acgatggata aaatactctc acaagacgaa atcgacgcgc tgttgaaagg ggtcgtctcc
     4201 ggcgaagttg agacccaggc aggggacgag ggtcaatcag ccggtgaggt caaacagtac
     4261 gacctcttta atcaagaacg catcgttcgc gggcgcatgc cgacgctcga gttgattacc
     4321 gatcggttta cccggcacca gtccatgtcc tggagcgcgc tgctgaatac gaaggtggag
     4381 ttcaatatta tcgggacgca aatcatgaag ttcggagcgt tcctcaagac cgttccgatt
     4441 ccctcctcac tgaacgtgtt tcgcatggaa ccgttgcggg ggaatggtct gttcgtcatg
     4501 aatgcgttct tggtctacct catcgtggac tatttctacg gggggaaggc gcaaacccat
     4561 gtgaagcctg agggccgcga tttcacgccg attcaattac ggatcatcaa gaagcttgtc
     4621 cagttggcgt ttacggacat ggagaaagcc tggcgcgccg cactctcggt caagaccgag
     4681 ttggtccgtt cagaagtcaa tcctcaattc gccatggtgg tctctgcgtc agaaatcgtc
     4741 ttcgtgacca ctcttcaagt tcaattcgga gagaccacca gcgatttctt tctggtgtat
     4801 ccctattcga tgctcgagcc cattaaagaa aaactctatt cgggcatcgt gtcggatcag
     4861 gttgagcaag acggaagttg ggccaccagg tttcatgaaa agctacaaga gtgcccgctt
     4921 gccgtgacgg ttcagctcgg aaccgcctcg atcaaggtac gagacctgtt ggacttcgct
     4981 ccgggagatc tcatcttatt ggaccaacgg cctggcgatc ccctcaattg ttttgttgaa
     5041 ggctatttga agtttcaagg aagccccggc gtctcgaagg ggaaccatgc ctgccgggtt
     5101 gacaaggtga tcgcgtaagc gactcacacg attgaggaga accatttatg gctaacaacg
     5161 aagtagcgaa tgccggagcg aacgctccca cccacacgcc ctcttcctct tcttcaccag
     5221 cgcctgcatc atttccatcg cttgacggag gcggagcggt cccagcgccc aagaatatcg
     5281 acttcattct tgacattccg atgctggtca ccgtgcaggt cgggtccacg aagatggtga
     5341 ttcgagagtt gctgcagctc gggcaggggt cggtgatcga actcgaaaag cttgcgggag
     5401 aaccgatgga agtgctggtc aacaacaagc tggtggcacg gggcgaagta gtcgtggtga
     5461 acgagaaatt cggcatccgt ctcaccgacg tcatcagcgc ggccgaacgc gttcagcaac
     5521 tcgcataggg gccattcaag gtatccctcg actaggactc atcgcagcat cctgctcggc
     5581 actgaggaac ggagatcacg tgatcgattt ttgggatagt ttcatacgaa tggcgtcggc
     5641 attggccctc gtgcttgccc tcatcggtgg gctcgtcctt ctggttcgtc gatttggcgg
     5701 cgcccggttg tccgcgcctg gatccgggca aattgttcag gtgatcggca gcgggtacat
     5761 cggcccgcgc aagacgattt ctttggtgtc cgt
//
LOCUS       DATIPF010000199         3053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1541777, whole genome shotgun sequence.
ACCESSION   DATIPF010000199 DATIPF010000000
VERSION     DATIPF010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541777"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /gene="dnaB"
                     /locus_tag="VLL06_05080"
     CDS             <1..1252
                     /gene="dnaB"
                     /locus_tag="VLL06_05080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HSS30372.1"
                     /translation="MAKAMELLVEDNFYRTSHRKIYRAMLELSDVGEVIDQITLTERL
                     KAQGEIEAIGGAAYLAELVQGVASSANIRYHCKIVRDKALLRELIHTSTEVLTRGYEG
                     TSSVDDLLDFAERSVFSIVQGKLDRSFTPLNSIIKESLDLVDKLSKRKEHITGVPTGF
                     YDLDDITAGLQPSDLVVIAGRPSMGKTSLALGMAVHAAIHAGTVVGIFSLEMSKPQIV
                     LRMLSSEARVDSHGLRTGKLQKEDWWRLAEAAGRLEQAPIYIDDTGGITVQQMRGKAR
                     RLKAERGLDLLIVDYLQLMQGRSDSESRQQEISDISRSLKALAKELNVPVVALSQLSR
                     AVEARKPPVPMLADLRESGAIEQDADVVMFIYREDVYDQNSERKGIADILVSKHRNGP
                     IGRKELFFHDRFAKFESLDNREVV"
     gene            1331..>3053
                     /locus_tag="VLL06_05085"
     CDS             1331..>3053
                     /locus_tag="VLL06_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS30373.1"
                     /translation="MKILHSNPLSFGLTISWQVTCASCMVLLLMAACAGAPKTPEAHA
                     LPQDGKVSIPAQRPPDAAASYHFMLGYQAELGQDMDRAIQEYQTALKADPSSQSVKAR
                     LAGLYFSLGDMSNAVRYADQAAEGLGQDGQMLTQMAGILASAGQGERAINLLDRAINL
                     DPTSSDPYFTKGLLLLNLKRQAEAEQTIRAGLARAPDSAVGYYHLGRILLDAGKGEEA
                     AASLERAIAVNASFEPAYLALASIYESQQAQDRAIAVLRKYLQTVNPRNRGIRHQLVR
                     IYIATKDYQGARNELNDLLAEDPSDLDAQLRLALLYGEEKEYPKAIDQLTKILKARPT
                     ELKIRDYLGYLYEESKDTKKAIETYTFNIQLEPSYFEGHLHLGMLFYRLKQFSEAITH
                     LTQALTINAKQPEAHIVLGLTYLQQDQFEEAAKVLEEGIKQNPKSADLYFNLGTVYDK
                     LNRFDDVVRVMETAIRLDPHHADALNYLGYSYAERGVKIDQALSLTKRAVALKPSNGY
                     YVDSLAWALFKSGMFTEALTEMKRAVALAGDDPVLYEHLGDIYAKQRNLSDAREAWLH
                     ALELDPSN"
BASE COUNT          704 a          802 c          875 g          672 t
ORIGIN      
        1 gatggccaag gcgatggaat tgttggtcga agacaacttc taccgcacgt cgcacaggaa
       61 gatctatcgt gcgatgctgg agttgtcgga cgttggagaa gtcatcgacc agatcacgtt
      121 gaccgaacgg ttgaaggcgc agggtgaaat tgaggccatc ggcggagccg cctatttagc
      181 ggaactcgtg cagggcgtgg ccagctccgc caatattcgc tatcactgca agatcgtgcg
      241 cgacaaggcg ctgttgcgag agctgatcca tacgtcgacg gaagtcctca cgcgcgggta
      301 tgaaggcacc tcctcagtcg acgacctgct cgatttcgcg gagcggtcgg tgttcagcat
      361 cgtccagggg aaattggatc ggtcgtttac ccccttgaat tccatcatta aagaaagtct
      421 cgacctcgtc gacaaactgt caaagcgaaa ggaacatatc acgggtgttc ctacgggctt
      481 ctacgatctc gacgacatca ccgctgggct tcaaccctcc gacctcgtgg tcatcgcggg
      541 gcgtcccagc atggggaaga cgagcctggc gctggggatg gcggtacatg ccgccatcca
      601 cgctggtact gtggtcggaa tttttagcct tgaaatgtcg aaaccgcaga tcgtcctacg
      661 tatgctgagt tcggaagcgc gggtcgattc acatggcctc aggaccggca agcttcagaa
      721 agaggattgg tggcgtctcg cggaggcggc aggccgattg gaacaagcgc cgatctatat
      781 tgacgatacc ggcggcatca ccgtgcagca gatgcggggt aaggcacgtc ggttgaaagc
      841 cgaacgggga ctggatctat tgatcgtgga ctatctccag ctgatgcagg gacggagtga
      901 ttcggaatct cgtcagcagg aaatttctga tatctctcgc tcgctgaaag ccttagccaa
      961 ggagctcaac gtaccggtcg ttgccctgtc gcagctgagc cgtgcggttg aagctcgaaa
     1021 gccacccgtt ccgatgctgg ctgacctccg cgagagcggt gcgatcgagc aggacgccga
     1081 cgtggtcatg tttatttatc gtgaggacgt gtatgaccag aactctgagc ggaagggtat
     1141 tgccgatatt ctcgtgagca aacatcgcaa cggcccgatc gggagaaaag aactcttctt
     1201 ccacgaccgc ttcgcaaagt tcgagagcct cgataatcgc gaagtcgttt gaccctgccc
     1261 gaaccccttt cgtatactct cagagtgtcg tgacgtgtcg gtcatcatcc gttatagcct
     1321 gtgcgtgttt atgaagatat tacactcgaa tccactttcg ttcggcctca cgatttcctg
     1381 gcaggtgacg tgtgcctcct gcatggtcct gttattgatg gcagcctgtg cgggtgcacc
     1441 caagacaccc gaggcccatg ccctacccca ggatgggaaa gtctctatac cggctcagcg
     1501 acccccagac gccgccgcgt cctatcattt tatgctgggg taccaggcag agcttgggca
     1561 ggatatggat cgtgccattc aggaatatca gaccgccctg aaggcagatc cgtcctccca
     1621 atctgtgaaa gcccgcttgg ccggtctgta tttttcgctt ggtgatatgt ccaatgccgt
     1681 acgttatgcg gatcaagcgg cagaaggact gggccaggat ggtcagatgc ttacgcaaat
     1741 ggcaggcatc ttggccagtg cgggacaagg agagcgtgcg atcaatctct tggaccgcgc
     1801 gatcaatctt gatccaacat ccagcgatcc ctattttaca aagggcctat tgctcctgaa
     1861 tctgaagcgg caggccgagg cggaacagac cattcgagct ggtttggcca gggctccaga
     1921 ctctgccgtg ggctattatc acctgggacg catactcctc gatgcgggga aaggggaaga
     1981 ggcggcggcg agtttggagc gggcgattgc ggtaaatgct tcgtttgaac cagcctatct
     2041 tgcgcttgcc tcgatctatg agtcacagca ggcacaggat cgggcgatcg ccgttctacg
     2101 gaaatatctc cagactgtga acccgcgaaa ccgaggcatc cggcaccaac tggttcggat
     2161 ctatatagcc acgaaggatt atcaaggggc caggaacgaa ttgaatgatt tgttggcgga
     2221 agatccgtcg gacctcgatg cccagcttcg actggcgctt ctgtacggag aagagaagga
     2281 atatcccaag gccattgacc aactgaccaa gatcctcaag gcgcgtccga cggagctcaa
     2341 aatcagagat taccttggat atctctacga ggaatccaag gacacgaaga aggccatcga
     2401 gacgtatacg tttaacattc aactagagcc gtcctatttt gaagggcatc tgcacctcgg
     2461 catgttgttc tatcgcttga agcaattttc cgaggccatc acgcatttga cccaagccct
     2521 tacgattaat gcaaaacagc ctgaagccca catcgtactg gggttgacct atttgcagca
     2581 ggaccagttt gaggaggcgg ccaaggtatt agaggaaggc atcaaacaga accccaagag
     2641 cgcagatctg tatttcaatc ttggcacagt ctacgacaag ctcaatcgat tcgatgatgt
     2701 ggtgcgtgtc atggaaactg ccattaggct ggatcctcat catgccgacg cgctcaacta
     2761 cttggggtat agctatgcag agcgtggggt taaaattgac caagcgcttt ccttgaccaa
     2821 gcgggctgtg gcgctgaagc cgagcaacgg atactatgtc gatagcctcg cgtgggcttt
     2881 gtttaaatcc ggcatgttta ctgaggcctt gacagagatg aagcgtgcgg ttgcgcttgc
     2941 tggagatgat ccagtgctct atgaacacct tggcgacatt tatgccaagc aacggaacct
     3001 gtcagacgcc cgcgaggcgt ggctccatgc gctggaactc gacccctcta ata
//
LOCUS       DATIPF010000200         3261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_657468, whole genome shotgun sequence.
ACCESSION   DATIPF010000200 DATIPF010000000
VERSION     DATIPF010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657468"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VLL06_05090"
     CDS             complement(<1..433)
                     /locus_tag="VLL06_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30374.1"
                     /translation="MTVAEADVSDTLVVGRVVTIITGESQRIYAPALRSFEVVNTESQ
                     ERYKVDVQSADGYFSLSLPAGHYELNRLQISEGPFLSMAQLSAGFSLKPDGMTFLGTW
                     RFGIDSPKYGRQVVVSMIQDGRDQERAQQFVRDTYPSLKTRS"
     gene            977..2458
                     /locus_tag="VLL06_05095"
     CDS             977..2458
                     /locus_tag="VLL06_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30375.1"
                     /translation="MRIRSILGALVIAALPFTSVWAENMTPEEIQKLVDDAVTKRLQE
                     HERREGAMERQEGPVGQQSEPGIGSLPDVAKERRADRQAALSFGSTGSGRLVYAKPFV
                     NAPKAIVGGYMDIQYRSHRKGVLEGYGNGTTNGFDQQRFIPFIYADVTEHVKFASELE
                     IEHGIREEGAGNEISLEFAHLDYLVNEPFNLRAGIILLPVGKFNLLHDSPLNDLTDRP
                     LVSQFVIPSTMAETGAGFYGTFYPGRTGKLDYEFYYTTGPCGYGPDGTPRITETEGTK
                     TSRQRNCASDDGLDINNGKAVTARVAYSPMLGVEVAGSSYYGNQSPTSYNPLSITAID
                     WTLQKGPFELIGEAAWAYARGNSRAIVGNTIGFTPGSLLTGATFNPFNPAAPPPQRMA
                     GFYIQGNYHFMPAFLTRLSPKRFGEGSTFTAVLRYDRVNLNMDNRGENSGEMEQISFG
                     LNYRPVEDAVFKLSYQYLPKSFNPITQQRIHDGALVLSAATYF"
     gene            2550..2987
                     /locus_tag="VLL06_05100"
     CDS             2550..2987
                     /locus_tag="VLL06_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30376.1"
                     /translation="MLTVLSAGCAMLSGSREQYFVCSYDTVWDVSLETMKGHSIASYD
                     KEKGVIETSWLDVPPITDRSFGIFGREGFGNKERARMTVSVKRIDDVASVSVLETRQR
                     WHSRGGVTSQALKWWAIDPSEEVTDDVVDRLNARLKAKGCAPA"
     gene            2984..>3261
                     /locus_tag="VLL06_05105"
     CDS             2984..>3261
                     /locus_tag="VLL06_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30377.1"
                     /translation="MMTRLLALSLALLLVMSPAMAWAERVWDSELKRYLTEQEMSMAE
                     VYLTEDEALKLMFPKSERVKKDLLRLPTDKKIAIEERIGWKFPEETFE"
BASE COUNT          794 a          872 c          883 g          712 t
ORIGIN      
        1 gagaccgagt cttcaggctg ggataggtgt ctctgacgaa ttgttgcgca cgttcctgat
       61 cccgcccgtc ctggatcatg gacaccacaa cttggcgtcc atatttcgga gaatcgatcc
      121 cgaatcgcca ggtaccgaga aaggtcatcc cgtccggctt gagcgaaaaa cccgcagaga
      181 gctgcgccat agacaagaat ggaccttcgc tgatctgcag gcggttgagc tcgtagtgcc
      241 cggcgggaag ggaaagcgaa aaatacccat ccgcggactg cacatccacc ttatatcgtt
      301 cctgactctc cgtattgacc acctcgaagg atcgaagcgc cggagcatag attcgttgcg
      361 actctccggt aatgatcgtg acgacccgtc ccacgaccaa agtgtccgag acgtcagctt
      421 ccgccactgt cacgggaagc tcagtcgcgc agcctgagag tatcaggcag agggcaccca
      481 gagacagcca ttgtcggtac tgtctgaacc ggctatacgg catacgtgaa cattataacc
      541 ctcctattag actgaagacc aacacatctc ttcgttcaca ccatctcttg acatcaatcg
      601 cgtgaatagg atattaataa tcgttctcaa tttcatctaa gcggagatat cttcgataat
      661 ggttcgcacg gcctctccca ccgaccactc caccgactgc agcgagaccc gttgcgaatg
      721 cggtcaactg atcgcgaagg tgcgcggtca ggggctggaa ctcaaatgca aacgctgcaa
      781 acgcattatc gtcatcccct tcacttccat caaaggatgg agcgcggtca cattatgatc
      841 gatctcgcga cgcaaggagt cttcacttcg gtgcatcgaa acgcagccta ttgttgtcga
      901 ttcagaacag agcagaccag agaccagcga gtcccgagtc agaccactca ccacatacaa
      961 tggatggagg ttcattatga ggatccggtc aatcctcggg gctctcgtga ttgcagcttt
     1021 accgtttacg tcggtgtggg ctgagaacat gacccctgag gagattcaga aattggtcga
     1081 tgacgccgtc acgaagcgac tacaggaaca tgaacgccgc gaaggtgcta tggaaagaca
     1141 agaaggtcct gttggccagc agagtgagcc gggtatcggt tccctgcctg atgtggcaaa
     1201 ggaacggcgc gccgatagac aggcggcgtt gtccttcggt tcaactgggt caggtcgatt
     1261 ggtctatgcg aaacctttcg tgaacgcgcc gaaggctatc gtgggcggct acatggatat
     1321 ccagtaccgg agccaccgaa agggagtcct agaaggctat ggcaacggga cgacgaacgg
     1381 tttcgatcag caacgattta tccctttcat ttatgcggac gttaccgaac atgtgaagtt
     1441 tgcttccgaa cttgagattg agcatgggat cagagaagag ggtgccggaa acgaaattag
     1501 cctggaattt gctcatttgg attatctcgt caatgaacca ttcaacctcc gggcgggtat
     1561 tatcctgctt ccagttggaa agtttaacct cctgcacgac tcgccgttga acgatttgac
     1621 cgatcggccc cttgtcagcc agttcgtgat tccctcaacc atggccgaga caggtgcggg
     1681 cttctatggc accttctacc ctggacgaac cggtaagctc gactatgaat tctattatac
     1741 gaccggtcct tgcgggtatg ggcctgacgg caccccgcgc atcactgaga cagaagggac
     1801 gaaaacctct cgccaacgga actgtgcatc cgatgacgga cttgacatca acaacgggaa
     1861 agcggtcacg gctcgtgtgg cctatagtcc gatgctcgga gtggaagtcg cgggatcgag
     1921 ctattacggc aatcaatcgc ccaccagcta taaccccctg agcattactg caatcgactg
     1981 gaccctgcag aagggaccat ttgagctcat tggtgaagca gcatgggcct atgcgagggg
     2041 aaactctcgt gcgatcgttg gtaatacaat cggctttacc ccaggctctc ttctgacggg
     2101 cgcgacattc aatccattta atcccgctgc tcctccccca caacggatgg cgggatttta
     2161 tatccagggt aactaccact tcatgccggc gttcctgacc agactctcgc cgaagcggtt
     2221 tggagaggga tccaccttca cggccgtgct tcgttatgac cgggtgaact tgaatatgga
     2281 taaccgtggc gagaactcgg gggaaatgga acagatctcc ttcgggttga actatcgccc
     2341 ggtggaagat gctgtgttca agcttagtta tcagtatttg ccgaagtcat ttaacccaat
     2401 cacccagcaa cgaattcacg atggagcgct ggtcctctca gctgcgacgt atttctaagc
     2461 tggcacaggc gatccctttc caccggaaag ggatcgcctg aagcaaggta gacatgtgca
     2521 caatgacgac atcccggata gccataatca tgctcacggt cctttctgcc ggttgcgcca
     2581 tgctgagtgg cagccgggaa caatattttg tgtgctccta tgacacggtc tgggatgtgt
     2641 cgcttgagac catgaaagga cattccatcg cgtcttacga caaagagaag ggtgtgatcg
     2701 agacaagctg gctggacgtc ccccccatca cagatcgatc cttcggtatc tttggacggg
     2761 aaggctttgg caacaaagaa cgcgcacgga tgacggtgtc cgtcaagcga atagatgacg
     2821 tggcctctgt gagcgtattg gaaactaggc aacgatggca ttcgcgcggc ggcgtcactt
     2881 cgcaagccct taaatggtgg gcaatcgatc cttcagaaga ggtgacggat gacgtcgtcg
     2941 accggctcaa tgccagattg aaggcaaagg ggtgtgctcc cgcatgatga ctcgattgct
     3001 cgcactctca ctggcgctcc tcttagtcat gtccccggcc atggcctggg ctgaacgggt
     3061 gtgggacagt gagctcaagc gctatctaac ggaacaagaa atgtcgatgg ctgaagtcta
     3121 cctcactgaa gacgaggcgc tcaaactgat gtttccaaaa tccgagcgcg tgaaaaaaga
     3181 tctgttgcgt cttcccacgg ataagaagat cgcgatagaa gaacgcatcg gctggaaatt
     3241 ccccgaagag acctttgaga t
//
LOCUS       DATIPF010000201         3640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_743737, whole genome shotgun sequence.
ACCESSION   DATIPF010000201 DATIPF010000000
VERSION     DATIPF010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743737"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2337
                     /locus_tag="VLL06_05110"
     CDS             <1..2337
                     /locus_tag="VLL06_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS30378.1"
                     /translation="YDSKDLVTHAVCVGMTGSGKTGLCLSLLEEAAIDNIPAIIIDPK
                     GDLGNLMLTFPSLKGEDFQPWINEDDARKKGLSPADFAKTQAELWAKGLGGWQQDGAR
                     IQRFRDAVEVAIYTPGSNAGLPVSILKSFAAPAADAHEDGELFRERISTTVTSLLGLL
                     GIEADPIQSREHILLSTIFDHIWRKEEDLDLAALIQAIQSPPASKIGVMDVDSFFPSK
                     DRFALAMKLNNLLAAPGFQAWLEGEALDIQSILYTPTGKPRLAIFSISHLNDAERMFF
                     VTLLLSQMVGWMRAQSGTTSLRALLYMDEIFGYFPPVANPPSKLPLMTLLKQARAFGL
                     GVVLATQNPVDLDYKGLANTGTWFIGRLQTERDKARVLEGLEGASSSAGKTFNKGRME
                     QTLAGLGARIFLMNNVHEDEPVVFETRWCLSYFRGPLTRTQIKRLMDPVRHEMLDVKP
                     ETPGTTLSARTASRPMLPPEVPQYFVPLRGSKPDGHELVYAPMLLGSSQIRFFDTKIG
                     IDTMRDITVFASISEGAVTVDWDHAVAADLTIADLAHEPEDLGQFLSLPVSAGKAKSY
                     ADWNKDFGGWLFRTQKVELLKSPSAKDVSKPGESERDFRARLQQSGREQRDKGAESLR
                     QKYAPKITALHDRIRRAEQAKERQQVESRSSQVQAAISVGASILGAFLGRKTISATNI
                     GRATTAIRGAGRAIKESQDVGRAEENVEALQQQLADLEAQFKSESESLAAATDPLNEK
                     FESIAIRPTKSNIAIKLVTLAWSPYWRDGRGALTQAWH"
     gene            complement(2549..>3640)
                     /locus_tag="VLL06_05115"
     CDS             complement(2549..>3640)
                     /locus_tag="VLL06_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30379.1"
                     /translation="APYSLLALLVVILMQAGCSSSVLHLREQLDLHYGEFREKLVDRK
                     ELETKFLACIGQAHDEPLSKWRDTRTPAGLLPPHTPPGQAMGIRSLATVIETIRHGHH
                     EKGASLSILADVLEDMADDVSPHIDLKKFTEVIETAGRWHGHFGLDEDKLEQDSSRFA
                     QMLLAYNKAYFGDLRFTARSDALGIDHRGVINVTSKGFVDRSGNTLLFPGISPEIVES
                     PARSIRMSTSTVNSQRVSADLTRIFLEAFFDTAYRVPAVHGATALRVRPNYQESSYPE
                     FDASHPVIPLEALARVTRDALRAEAAVTSLVGRAVRGGSVFGTQNETLAASLETAAGV
                     IAKKFVEHEGFCYFQVTIVADDQNEPNAR"
BASE COUNT          815 a         1014 c         1037 g          774 t
ORIGIN      
        1 tacgattcga aagatttggt gactcacgcc gtctgtgtcg gcatgacagg cagcggcaag
       61 accggtctgt gtctctcact tctcgaagaa gcggcgatcg acaatattcc cgccatcatc
      121 atcgatccta agggcgacct cgggaatttg atgttgacct ttccaagtct gaagggcgag
      181 gattttcagc cctggatcaa tgaagacgat gcgcgcaaga aaggattgtc accagccgac
      241 tttgcaaaga cacaggcgga gctctgggcg aagggattgg gaggctggca gcaggacggc
      301 gcgcgcatcc aacgtttccg agacgcggtc gaggtcgcca tttacacgcc gggcagcaac
      361 gcagggttgc ctgtctccat tttaaaatcg tttgccgcgc cggctgcgga tgcccacgag
      421 gatggggagc tgtttcgcga gcgcatcagc accaccgtaa cgagcttgct ggggttgctc
      481 ggcatcgaag ccgatccgat ccagagccga gagcatatcc tgctttcgac gattttcgat
      541 cacatctgga ggaaggagga agacctcgac ctcgccgcac tcattcaagc aatccaatcg
      601 ccgccggcct cgaagatcgg tgtgatggat gtcgattcgt tcttcccctc gaaagatcgg
      661 tttgcgttgg caatgaaatt gaataatctg ctcgcagcgc ccgggttcca ggcctggctg
      721 gaaggagaag cgctcgatat tcagtcgatc ctctataccc cgacaggcaa gccgcgcctg
      781 gccatctttt ccatctcgca tctgaacgac gccgaacgca tgttcttcgt gacactcttg
      841 ctcagccaga tggtgggctg gatgcgggca caatcgggca caacgagcct gcgtgccctc
      901 ctctatatgg atgaaatctt cggttacttc cctccggtgg cgaacccgcc gtcgaaactg
      961 cccttgatga ccctgctcaa acaggcgcgc gcatttggtc tgggcgtcgt cctcgcgaca
     1021 cagaatccgg tcgacctcga ctacaaagga ctggccaata ccggcacttg gttcattgga
     1081 cgattgcaga cggagcgtga caaggcccgg gtactcgaag gattggaggg ggcttcatcc
     1141 agcgctggga aaacgttcaa caagggtcgg atggaacaaa cactggcagg gcttggtgcc
     1201 agaattttct tgatgaacaa cgtacacgaa gatgagccgg tggttttcga aacccgttgg
     1261 tgtctgtcgt actttcgcgg accgcttacg agaacccaga tcaaacgctt aatggaccca
     1321 gtaagacacg aaatgttaga cgtgaaacct gagacgccag ggacaacgct atcggctcgg
     1381 accgcatccc gtcccatgtt gcctcccgag gtgccgcagt actttgtgcc cctgcgcggc
     1441 tccaagcctg acggacacga actggtgtat gcgccgatgc tgttaggatc ttctcagatt
     1501 cgctttttcg ataccaaaat cggcatcgat accatgcggg acatcacggt gtttgcatcg
     1561 atttcagaag gggctgtgac ggtggattgg gaccacgcgg tcgcggctga tctgaccata
     1621 gctgatttag ctcacgaacc ggaagacctc ggccagttcc tttcactccc ggtcagtgcc
     1681 ggcaaggcca agtcttatgc cgattggaac aaggatttcg gaggctggct cttccgcacg
     1741 caaaaagtgg aattgctcaa gagcccgagc gcgaaggatg tatccaagcc gggagagtcc
     1801 gagcgagatt tccgcgcgag gctccaacaa tcaggccgcg agcaacgaga caaaggagcg
     1861 gagtcgcttc gtcaaaagta cgcgcccaag atcacagccc tgcacgatcg cattaggcgg
     1921 gcggagcagg cgaaagaacg gcagcaggtg gaatctcgct ccagtcaagt gcaagcagcc
     1981 atttcagtcg gtgcgtcgat cctcggcgcc tttttgggtc gcaagacaat cagcgccacg
     2041 aacatcggcc gggctaccac cgcgattcgt ggagccggtc gcgcgatcaa agaatcccaa
     2101 gatgtcggtc gagccgaaga gaatgttgag gcgcttcagc agcagctggc agacctcgaa
     2161 gctcaattca aatccgaaag cgaatcgctc gcggctgcca ctgatccctt gaatgaaaag
     2221 ttcgaatcga ttgcgatcag acccaccaaa tccaatatcg ctataaagct ggtcaccctt
     2281 gcctggtccc catattggcg ggatggtaga ggtgcactca cacaggcttg gcactaggca
     2341 ggaaacagct ggtctcgcat aggtgtgcct gtcgaagacc gacgtgtcgc aggatcctga
     2401 aaacgtccgt cagcgtcgtt ttcgctatgc gtagaaactt caccttaggc aggagacgac
     2461 ctccttcacc tacaaggtcg tctggtatgg ggctttctgt ttacgacata cttcttgact
     2521 atgattctct cgacggtccc gcccatggtt accttgcatt cggctcattc tgatcatccg
     2581 cgacgatcgt aacttggaaa tagcagaacc cctcgtgctc gacgaacttc ttcgcgatga
     2641 ccccagctgc ggtctcaagc gaagccgcca gcgtttcatt ctgcgtgccg aagacacttc
     2701 caccacgaac cgctcttccc acaagcgatg tcacggcggc ttcggcacgc aaggcgtcac
     2761 gggtcactcg ggctaaggcc tccaatggga tcacgggatg gctcgcatcg aattcagggt
     2821 aggatgattc ctgatagttc ggtcgcactc gtagtgcggt cgcgccatga acggccggta
     2881 cccgataggc agtgtcaaag aaggcttcca gaaaaatacg tgtcagatcg gcactgacac
     2941 gttgggagtt gaccgtgctg gtcgacattc gaattgaccg ggcagggctt tctactattt
     3001 ctggcgagat tcccgggaag agcagggtat tgccgcttcg atcgacgaac cctttagaag
     3061 tcacgttgat gaccccgcga tgatcgattc ccaaggcgtc cgatcgtgct gtgaagcgaa
     3121 gatcgccaaa atatgctttg ttgtacgcca gcagcatctg ggcgaatcga gaagagtcct
     3181 gttcgagttt gtcctcatcc agaccgaagt gcccatgcca tcgcccggcc gtttcaataa
     3241 cctctgtgaa tttcttcaga tcgatgtgcg gactcacgtc atcggccatg tcttcaagga
     3301 cgtctgcgag gatggagaga gacgcgcctt tctcgtgatg cccatggcga atcgtttcga
     3361 tgacggttgc cagggaacgg atacccatcg cttgtcccgg aggggtatgc gggggcagca
     3421 gtccggccgg ggtacgggta tcacgccact tgctcaatgg ttcgtcgtgg gcttgcccga
     3481 tacaagccag gaactttgtc tcgagctcct tcctgtcgac caacttctcg cggaactctc
     3541 cgtaatggag atccagttgc tcgcgaagat gtaggactga agaactgcag cctgcttgca
     3601 tgagtatgac gaccagcaag gccaacaggg aatagggtgc
//
LOCUS       DATIPF010000202         5108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_392332, whole genome shotgun sequence.
ACCESSION   DATIPF010000202 DATIPF010000000
VERSION     DATIPF010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392332"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VLL06_05120"
     CDS             <1..366
                     /locus_tag="VLL06_05120"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HSS30380.1"
                     /translation="GHPGALLSSAKQPSEWVTHASFPLGFDPSARFASDRVVLKPGDR
                     LFLLSDGIYEVPSPSGELWGRGRFREAIDAGRQKSLSAAVASCFSEARGWQQAQHFPD
                     DAAVVGLELIGNPEGRFNG"
     gene            359..730
                     /locus_tag="VLL06_05125"
     CDS             359..730
                     /locus_tag="VLL06_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HSS30381.1"
                     /translation="MDKTMDKMAVLDLSGALRRADGDQDLFLILAGLFLQESPKEAAA
                     AREALERQDRAGLAAAAHKLKGSVAEMCAPRLFESTKRLEELGRLGEFAEALPVCADV
                     EIRLAEVHAALRELIAGGFPS"
     gene            727..1638
                     /locus_tag="VLL06_05130"
     CDS             727..1638
                     /locus_tag="VLL06_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS30382.1"
                     /translation="MTPTAGLHTQARTLLVVADNADTQTLILAHAKAQGHSVISASSP
                     ALGLTTFDMAQPDIVITDLFLPEEDGIMLVKQIRDRRPTCPVVLLTDAGEIESTMEGL
                     RAGALDYVEQPIYEDAFAQVLMRAIHSLPVSVDDAPGVERLEYVLVMDPDPSYVEGTV
                     TWLIQGTAMGLAEARQLHLRAALQELVMNAVEHGSLELQYRDKIDAVAKDQYDELIRD
                     RRQGSRFRDRRVTIRAIYDKKQQVLTYQISDEGKGFNWRTQMGSRYQACPSVDASGRG
                     VFLARSFFPDLAYNDRGNEVTFTVPFG"
     gene            1782..2150
                     /locus_tag="VLL06_05135"
     CDS             1782..2150
                     /locus_tag="VLL06_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HSS30383.1"
                     /translation="MDTSNPTRSKLRIPHDAMYLLLREGCITEFNVKKTSGETVDLRG
                     CDLRGLDLRGLAADGLDFSDCYFRQSDLRSVDFRKARLEGASINGAKISGAYFPAELT
                     ASEIELSLLHGTRMRYRSDK"
     gene            complement(2286..3665)
                     /locus_tag="VLL06_05140"
     CDS             complement(2286..3665)
                     /locus_tag="VLL06_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSS30384.1"
                     /translation="MGHEPVTAENGKDALRLIEQGDFDLVLLDLELPVLSGLEILERV
                     KGTSHINRRTEEDIHAADGTPFIIILTAFGTIERAVHAMQLGAFDFMTKPFSADHLTV
                     VVNKALATAALYRQVDVLRKEVDDRYDHLVGANAKMAAQLTIARQAAASDVTVLLLGE
                     TGTGKEVVARAIHRWSPRRSKPFIAVNCAALPENLLENELFGHEKGAFTGAVKREPGK
                     IEVGEGGTVFLDEIGDMPLPLQSRLLRVIQDQTFYRVGGTQSVHTNVRFLAATNKDIR
                     RMIKQGTFREDLYYRLAVITVTLPPLRERIDDVPALAQHFLNRAVKMGVHGSCTLSDR
                     AVQALQQYAWPGNIRELDNVLTRALILCADDTLEPAHLHLADSLPLSTTETETEAGSR
                     SRHYHDLMAAYSRKIIEEALHRNGGNRTRASEDLGLQRTYLTKLLRQKEISTEPPKDF
                     SPSSEVDAP"
     gene            complement(3804..>5108)
                     /locus_tag="VLL06_05145"
     CDS             complement(3804..>5108)
                     /locus_tag="VLL06_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HSS30385.1"
                     /translation="AIVGTLDRGQLLSTVLQLLIENLGFTRMFLMLRHRDRETAYVAQ
                     VAGVSPDIEAVAHQLDLPIQDDGGLQADLLIHGKPLLILSLDAVADRMQPPVLDLARR
                     TGVTSFVAVPLQSHNQMLGYLAADRGAQPCTEEDLHMLLTIASHVAAAIDNARAYSHL
                     EELTQHLEQRIQERTQELSVANERLQEHDRRRSLFLSVASHELRTPMTVIRSFADNMR
                     DGIAGPVSEQQLIYLTRIGHNMNRLTRIINQLLDWSRLDSKRDMLFLTPIHVETTALL
                     VADSLRTVAAEKMITIETVHSDGLPPVRGDQDKLEQILWNLIGNAIKFTPPGGRITVD
                     FKTTPEGFVQTSVADTGCGIDPDHMEKLFQEFSKVPSPNPTAQGAQLGLFITKSLVTM
                     HQGTIWVESTPGAGTRFYFTLPIAPSPEDSGATGSATGRVNE"
BASE COUNT         1037 a         1332 c         1592 g         1147 t
ORIGIN      
        1 ggccatcccg gcgcgctgct cagctctgcc aagcagcctt cagaatgggt cacgcatgca
       61 tcgtttcctc ttggtttcga tccatccgcg cgatttgcca gcgaccgtgt tgtcctgaag
      121 ccgggcgacc ggttgttttt attgagcgat gggatttacg aagtgccttc tccgagcggg
      181 gaactctggg gtcgcgggcg attccgggag gcgatcgacg cggggaggca gaagtctctc
      241 tcagcggcgg ttgcgtcgtg tttttcagag gcccgaggct ggcaacaggc gcaacatttt
      301 ccggacgatg cggcggttgt ggggcttgaa ctcatcggta acccggaggg gagattcaat
      361 ggataagaca atggataaga tggcggtgtt ggatctttcc ggggcattga ggcgagcgga
      421 tggagatcag gacctattcc ttatcttggc cgggctgttt ttgcaagaga gtccgaagga
      481 agcggcggcg gctcgtgagg cgctggaaag gcaggaccga gctggtttgg ccgcggcggc
      541 gcataagctg aaggggtccg ttgcagagat gtgcgcaccg cgtctcttcg agagcacgaa
      601 acgattggaa gagttgggcc ggttgggaga atttgccgag gcgttgccgg tctgtgcgga
      661 tgtcgaaata cgcttggccg aggtgcatgc cgcgcttcgg gaattaattg ccggaggatt
      721 tccatcatga ctcccacagc aggattgcat acacaggcgc gtaccttgct tgtcgtggcc
      781 gacaatgctg atacccagac cctgattctg gcgcatgcga aggcccaggg gcattcggtg
      841 atttctgctt cgagccccgc gctcgggttg accaccttcg acatggcgca gcccgatatc
      901 gtgattacag atctcttttt acccgaagag gacgggatca tgctggtgaa gcagattcgc
      961 gatcgtcgtc cgacctgtcc ggtcgtgctc ctgaccgacg caggcgaaat cgaatcgacg
     1021 atggaagggt tgcgcgccgg tgccctcgat tacgtggagc agccgattta tgaagatgca
     1081 ttcgcacaag ttctgatgag ggcgattcat tcgctgcctg tgtcggtgga tgatgcgccg
     1141 ggcgtggaac ggctggagta cgtgctggtc atggatccgg atccgagtta tgtcgagggc
     1201 acggtgacct ggctcattca ggggacggcc atgggtctgg cggaagcgcg acagctccat
     1261 cttcgggccg cgttgcaaga acttgtgatg aatgccgtcg agcacggcag cctcgagctt
     1321 cagtatcgtg acaagatcga cgcggtggcc aaagaccagt atgacgagct gatccgggac
     1381 cgcaggcagg ggagtcgctt ccgggaccgc cgggtcacca tccgtgccat ctacgacaag
     1441 aaacaacagg tgttgacgta ccagatttcc gacgaaggga aggggttcaa ttggaggaca
     1501 cagatgggct cccgttatca ggcctgccct tcggttgatg ccagcgggcg aggtgtcttt
     1561 ctcgcacggt cgtttttccc tgatctcgca tataacgaca gaggaaacga agtcacgttc
     1621 acggttccct ttgggtgacc gttgcacgag ccacatgatt catgaacagg tctactgcaa
     1681 tcgtcgatcc gcggctgcga tgggccgtca tgaatcatat gggttaagtc cacggggaat
     1741 gttccgataa ctatcaaagg gaatgccacg acggagaaga catggatacc agcaacccta
     1801 ctcgctccaa actgcgaatc ccgcacgatg cgatgtatct gttgctgcgc gaagggtgca
     1861 tcaccgaatt caacgtcaaa aagacatcag gcgagaccgt cgacttgcgg gggtgcgatc
     1921 tgcggggtct cgacctgcga gggttggctg ccgatggatt ggactttagt gactgttact
     1981 ttcgccaatc ggatctccgg agtgtcgatt ttcgcaaagc gcggcttgag ggtgccagta
     2041 tcaatggggc gaaaatttca ggcgcatact ttccggctga gctgactgcc agcgagattg
     2101 aactgtccct tcttcacggc acccgcatgc gctaccgctc ggataaatga ctggtctgtc
     2161 tggtctattt ggtttgtctt gtttgttttc ttggtttggt tcgaagaact aagaaaccaa
     2221 ccgagtcacc taaccagaca aaccagatag accagagaaa ccagacgaac cgaatcaacc
     2281 aaaattcagg gtgcgtctac ctcggaggat ggggaaaagt ctttgggcgg ttcggtggag
     2341 atttccttct gacggagcag ctttgtgagg taggtccgct gaaggccgag atcttcggat
     2401 gctcgtgttc ggttccctcc atttctgtgc aaggcctctt cgatgatttt ccggctgtag
     2461 gctgccatga ggtcatgata gtgacgggac cgagatcctg cctcggtctc ggtctcggtg
     2521 gtcgagagag gcagggaatc cgccagatgc aggtgggcgg gctcaagcgt gtcatccgca
     2581 cacaagatga gcgcgcgtgt cagcacattg tccagttcgc ggatgttgcc aggccatgca
     2641 tactgttgca atgcctgtac agcgcggtca ctcaatgtgc aggagccatg tactcccatt
     2701 ttcaccgcac gattgagaaa atgctgggcg agtgcaggga catcgtcgat acgttcacgc
     2761 aggggtggta gcgtcacggt gatcaccgcg agccggtagt agagatcctc acgaaatgtg
     2821 ccctgtttga tcattcgccg tatgtctttg ttcgtagcgg cgaggaaacg gacattggtg
     2881 tggaccgact gggttccgcc gacgcgatag aaggtctgat cctggatgac ccgcagcagg
     2941 cggctttgta gcggcagcgg catatctccg atttcatcca gaaacaccgt tccgccttct
     3001 cccacctcga tttttcccgg ttcccgtttg accgccccgg tgaaggcgcc cttttcgtgg
     3061 ccgaacagtt cgttctcgag caagttctcc gggagcgcgg cacaattgac tgcgataaac
     3121 ggtttcgagc ggcgtggact ccagcgatga atggcgcggg ccacgacttc cttccctgtc
     3181 ccggtttcac cgagcaggag gacggtcacg tcggacgccg ccgcttgtct ggcgatcgtg
     3241 agttgggcag ccatcttggc gttggcccct acaaggtgat cgtaccggtc gtcgacttct
     3301 tttcggagga cgtcgacctg tcggtacagc gccgcggtcg ccagggcttt attgaccacg
     3361 acggtcagat ggtcggcact gaacggtttc gtcatgaaat cgaatgcgcc aagctgcatg
     3421 gcgtgaacgg cgcgctcgat ggtgccgaat gcggtcagga tgatgatgaa gggtgtaccg
     3481 tcggctgcgt ggatgtcttc ttcggtgcgg cgattgatgt gcgaggtgcc ttttacccgt
     3541 tccaggattt ccagaccgga caggaccggt agttccaaat ccagcagtac gagatcaaag
     3601 tctccttgtt caatcagacg cagcgcatcc ttaccgttct cggcagtgac aggttcgtgc
     3661 cccatctagg tgatacgatt ctcgagtccg agaagaatat cgcgatcgtc gtcaacaatg
     3721 agaatttttg cgcgcatggt gtctgtggag acggtaatta ccggcgaaat tgtagatcat
     3781 atgcacaaga aaatatactc ctgtcactca ttcacgcgtc ctgtggcgga gcccgtggct
     3841 ccggaatctt ctggtgacgg cgcgatgggc agggtgaaat aaaagcgcgt cccggctccg
     3901 ggcgtactct ctacccagat ggttccctga tgcatcgtca ccaggctttt ggtaatgaac
     3961 aagccaagct gagctccctg agcggtgggg ttgggagacg gcactttcga aaactcttga
     4021 aagagctttt ccatgtgatc cgggtcaatc ccacagccgg tatcggcgac agaagtttgg
     4081 acaaagccct caggagtcgt tttgaaatcg acggtgatac ggccgcctgg cggcgtgaac
     4141 ttaatcgcgt tgccgatgag attccagaga atctgttcca gtttatcctg gtccccccga
     4201 actggtggga gcccatccga atggacggtc tcgatcgtga tcattttctc agccgcaacg
     4261 gtgcgcagac tgtcggcgac cagaagggct gtggtttcga catggatcgg tgtgaggaac
     4321 aacatgtccc ttttcgaatc aaggcgggac caatcgagca attgattgat gattcgagtg
     4381 agtcgattca tattgtgccc gatgcgagtg agatagatta gttgttgttc gctcacgggt
     4441 ccggcaatgc cgtcgcgcat gttgtctgca aaactgcgga tcacggtcat gggggtgcgc
     4501 aattcatgcg atgcgacaga gagaaacagc gaccgccgcc ggtcgtgttc ctggagccgt
     4561 tcattggcga cggacaattc ctgcgtacgc tcctggatac gctgttcaag gtgctgggtg
     4621 agttcttcaa ggtgagaata ggctctggcg ttgtcgatgg ccgcggccac atggctggcg
     4681 atcgtcaaca acatgtggag atcctcctct gtgcagggct gagcgccacg gtcggcggcg
     4741 aggtatccga gcatctggtt gtgactctgg agcggcacgg cgacgaagga cgtcactcca
     4801 gtgcggcggg ccaagtccag taccggtggc tgcatgcgat cggccacggc atccaggctt
     4861 aggatgagca ggggcttgcc gtgaatcaac aggtcggctt ggagcccccc atcgtcctgg
     4921 atcggcaggt cgagctggtg agccaccgcc tcaatgtccg gcgaaacccc ggcgacttgg
     4981 gccacatacg ccgtttctcg atcgcgatgc cgcaacatca agaacatccg tgtaaaccca
     5041 agattctcga tcagaagctg caggacagtg ctgaggagct ggcctcggtc caatgtcccg
     5101 acgatggc
//
LOCUS       DATIPF010000203         7330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1100186, whole genome shotgun sequence.
ACCESSION   DATIPF010000203 DATIPF010000000
VERSION     DATIPF010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7330
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100186"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VLL06_05150"
     CDS             <1..675
                     /locus_tag="VLL06_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HSS30386.1"
                     /translation="QSHDPREIEPVKRVAARIIEAAKRSKYAEIAQEFQWEVTIIKDD
                     KTMNAFALPGGKIAVYTGIFPVARTEAGLAAVLGHEVVHALARHGAERMSQGQLTNVA
                     LQAAGAAAGAAGGGGMLSQAAMAALGAGAQVGILLPYSRKHESEADYIGILLAADAGY
                     DPRESVLLWERMGQLSGGASPSEFLSTHPNHETRIIQLKKWMPEAMAIFQTKPPVPAA
                     LLPALQ"
     gene            742..1169
                     /gene="rnpB"
                     /locus_tag="VLL06_05155"
     ncRNA           742..1169
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VLL06_05155"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1683..3458
                     /gene="ilvB"
                     /locus_tag="VLL06_05160"
     CDS             1683..3458
                     /gene="ilvB"
                     /locus_tag="VLL06_05160"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HSS30387.1"
                     /translation="MKLTGAEILIECLKREGVKTIFALPGGVVLKIFDMLHQQKDIEV
                     ILTRHEQGAGHMAEGYAKATGKAGVCLVTSGPGMTNVITALADAYMDSVPLVCFSGQV
                     PTSLIGNDAFQEADNVGLSRPCTKYNFLVKNVTELATTIKEAFYIATTGRPGPVLVDI
                     PKDVSMDKTEFTYPSSVSIRSYNPVYEGNKWQIKQAAEAMTKAQKPVLYVGGGVVFSG
                     ASQELVELAELTHMPVDMTLMGLGAFPGEHPQSLGMLGMHGTYWANMAMHYSDLVVAI
                     GARFDDRVTGKVSEFCPHAKVIHIDIDPTSIRKNVNVDIPIVGDCKKVLRELIQILRA
                     TVNGDQKELRKAWWHQIREWQQAHPLAYQQEADGPIKPQHVIKRLYELTKDRDPIVST
                     DVGQHQMWAAQYFKLAKPNRWLTSGGLGTMGFGFPAAMGAQAAFPGRLVLCIAGDGSI
                     QMNMQEMATAVVHKLPVKIIVLNNRFHGMVRQWQDLFYEGRYASSYLETTPDFVKLAE
                     AYGSVGFRASTPSDIDAVLKEMIAVDKPVIVDVQTYPYENVYPMIPAGGCNHEMILED
                     PPALKNKQPGAGQVAPKDSDTILTA"
     gene            3486..4004
                     /gene="ilvN"
                     /locus_tag="VLL06_05165"
     CDS             3486..4004
                     /gene="ilvN"
                     /locus_tag="VLL06_05165"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HSS30388.1"
                     /translation="MEHIISITVENKFGSLSRVAGLFSGRGFNIESLSVAPTLDPSMS
                     QMTIVTSGDERIIEQIVKQLNKLIDVIKVVDLNESDFVSRETALIKVHTRPEDRAEAL
                     RIADIFRANVIDSTPATYTIEVTGDPRKIEAIINLLQPLGIKELTRTGRVAVARETIR
                     STGVQAKKMARE"
     gene            4072..5085
                     /gene="ilvC"
                     /locus_tag="VLL06_05170"
     CDS             4072..5085
                     /gene="ilvC"
                     /locus_tag="VLL06_05170"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HSS30389.1"
                     /translation="MKIYYDKDADIQQIRNKKVAVIGYGSQGHAHALNMKESGVSVVI
                     GLREGASWKKAEQSGLKVMPVADAVKTSDVVMILAPDEAQAAIYRQDIAPNLKPGSSL
                     AFGHGFNIHFGQIVPPATINVFMVAPKGPGHLVRSEYTKGSGVPCLLAIHQDPSGTTK
                     QVGLAYASAIGGGRAGVIETNFREETETDLFGEQVVLCGGLTSLIQAGYETLVEAGYS
                     PEMAYFECLHEVKLIVDLIYQGGIANMRYSISTTAKYGDITRGPRIVTEQTKQEMKKI
                     LSEIQQGQFAKEWVLENQANRPVYNALLAKGEAHPIEEVGGRLRAMMPWLKKDQLVDK
                     NKN"
     gene            5099..5749
                     /locus_tag="VLL06_05175"
     CDS             5099..5749
                     /locus_tag="VLL06_05175"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HSS30390.1"
                     /translation="MADRAVGIPFAKEGIPFIGAAVGVTLLAVWLGWIIVAVGAAILT
                     LFVSWFFRNPSRVVPQGPHLVVASGDGKVIAIEEEFEPRFIKDRSIRLTIFLNVFDVH
                     INRIPCEGVVEEIRYQPGLFRVASRPDATLQNEQNALMIRTVEGGKVLCVQVAGLIAR
                     RIVCWISPGDQAVRGERFGLIRFGSRMDTFLPIGTTVKVAVGDRVKGGETILGELP"
     gene            5746..6549
                     /gene="pssA"
                     /locus_tag="VLL06_05180"
     CDS             5746..6549
                     /gene="pssA"
                     /locus_tag="VLL06_05180"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HSS30391.1"
                     /translation="MKANGLRQSFGKDGKRRKAAMHLIPNLFTTGNLLCGVFSMLSVF
                     NADYMVAAVAILVAMVFDVLDGKSARLTNSTSHFGLEYDSLSDVVSFGVAPGLLIYSW
                     ALSGQGTFGIAVMFAYVAMGAVRLARFNSAAAVADGKYFTGLAIPAAAGVVASLVIFD
                     HYSVRIGTEVKPMLVLIITLTLSFLMVSTIKYRSFKDMKFRGGQHITYLVWGILGLML
                     IAAWPQVMLFVIFAGYALLGPAERLVGLLFKITGKRPSPKAAQSVIESK"
     gene            7003..>7330
                     /locus_tag="VLL06_05185"
     CDS             7003..>7330
                     /locus_tag="VLL06_05185"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247590.1"
                     /note="catalyzes the formation of 2-isopropylmalate from
                     acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HSS30392.1"
                     /translation="MTRMIRIFDTTLRDGEQSPGASMNVEEKIMVAKQLARLGVDIIE
                     AGFAYSSPGDFTAVQRIAQEVEGPTICSLARARPEDIERAWEALKGAPKVRIHTFLST
                     SDIHLKH"
BASE COUNT         1646 a         1783 c         2231 g         1670 t
ORIGIN      
        1 cagtcacacg atccacgaga gattgaaccg gtcaagcggg ttgcggctcg gattatcgag
       61 gcggcgaagc gatcgaaata tgctgagatc gcgcaggagt ttcagtggga agtgacgatc
      121 atcaaagacg acaaaaccat gaacgcgttc gctctgcctg gcggaaagat cgcggtctat
      181 accgggattt tccctgttgc acggactgaa gcgggattgg ctgcggtgct gggacatgaa
      241 gtcgtccacg ctcttgcgcg tcatggcgcg gaacggatga gtcagggcca gctgacgaat
      301 gtggcactgc aggctgcagg agctgcggcc ggggcagcag gtggtggagg gatgctttct
      361 caagccgcga tggcagccct cggtgcgggc gcgcaggtag ggattttgtt gccgtacagt
      421 cggaagcatg aatcggaagc agattacatc ggaattcttc ttgcggccga tgccggatac
      481 gatccacgtg agtccgtctt gctctgggaa cgtatggggc agttatccgg gggcgccagc
      541 ccgtccgagt ttttgtcgac tcacccaaac catgagacga ggatcatcca gttgaaaaag
      601 tggatgccgg aggccatggc catttttcag actaagccgc cggtgcctgc ggcgctgttg
      661 ccggccctgc agtaagccga ggcaattcgc ggtgagccct tgaaagcttg gagatccagt
      721 cctatagtac cctggcgcga gggagaagat tgggtgatcg ctcggagcgg taacgctttg
      781 agaggaaagt ccggacttca tgggcaaggc gctgggtaac tcccaggcgg agtgatccga
      841 caccagcacc acagagaaca aaccgccgat ggccaaggcg agggtatttc aatccgcttg
      901 ccttggctca ggtaagggtg aaacggtggg gtaagagctc accgcagtgg tggcgacatc
      961 actggcaagg taagcgtcgc cagatgcaag gccaaatagg aagacacctg tcggctttct
     1021 ttggagagtc ggcgaccggc tggcccggcc gaggtctttg ggtaggtcgc ttgaggttcg
     1081 aggtaactcg gatcccagag aaatgatcat ccccgtttag gagaaaatct caagcgggac
     1141 agaatccggc ttataggtct tctccaccgc gcttttttta tcgcttgacg gagttcccct
     1201 tccgttactt tccctgcgaa gtgttcaaca tcgtcgagca agacgacagg aacccttgtc
     1261 ccatagcgct ctgacaaaac cctatcgtgt tcaatcggca ccttatgtgt ttcaatatga
     1321 agctcagact gaagacgtag cgcgattcgg tgcacgactt cgcacagatg gcagtcttca
     1381 cgagacagga tcgtcacacg aataagcatg ctcatggagg cgtggcccag ggccaacgaa
     1441 ttggagcctc gagggtatca cgtggggagg aagactgtta gtgagaggct cccaagcgac
     1501 catactattg acgagtggaa acacgtggtg ataggatcac atgtctttct cccctgattt
     1561 ttcagaatgt tctgaatagc tgtggaaggc gtctctggtc catttgggac gagaggcgct
     1621 gtatcgcatt ctcctgattc ctctaccagg ttggtactgg cggtatggat ggaggttctg
     1681 tcatgaagct cacaggtgct gagattctca tcgaatgcct caaacgggaa ggcgtcaaga
     1741 cgatctttgc gcttcccggt ggcgtcgttc tgaagatttt cgacatgctt catcagcaga
     1801 aggatattga ggtgattctc actcgccatg aacagggtgc gggtcatatg gcagaaggct
     1861 atgccaaggc gaccggtaaa gccggggtgt gccttgtgac ttctggcccc ggtatgacga
     1921 acgtcattac ggcattggcg gatgcctaca tggattccgt accactggtc tgtttcagcg
     1981 ggcaagttcc taccagtctt atcggaaatg atgcattcca agaggcagac aatgtcggtt
     2041 tgagccgccc ctgtaccaag tacaactttc tggtgaaaaa tgtgaccgag ttggcgacga
     2101 cgattaaaga agcattctac attgcgacga ctggacgtcc aggtcccgta ttagtagaca
     2161 ttccgaaaga cgtatctatg gataagacgg agtttacgta tccgagttcc gtctccatcc
     2221 gtagctacaa tccagtgtat gaagggaaca agtggcaaat caagcaagcg gctgaggcga
     2281 tgacgaaggc acagaagccg gtgttgtatg tcggcggcgg cgtggtcttc tcgggcgcgt
     2341 cacaggaatt ggtggagttg gcagagctga ctcatatgcc ggtcgatatg acgttaatgg
     2401 ggctgggggc ctttccgggc gagcacccgc agtctttggg catgttgggc atgcatggaa
     2461 cgtattgggc aaacatggcg atgcactatt ccgaccttgt ggtcgccatc ggtgcaagat
     2521 tcgatgatcg cgtcaccggg aaagtgtccg aattttgccc acatgccaaa gtcatccaca
     2581 tcgatatcga tccgacctcg attcggaaaa atgtcaatgt cgacattccg atcgtcggcg
     2641 actgcaaaaa ggtgcttcgt gagttgattc agatacttcg tgccacggta aacggcgatc
     2701 agaaagaact gcgcaaggcc tggtggcatc agattcgcga atggcaacag gcgcatccgc
     2761 ttgcctatca acaagaagcg gatgggccga ttaaaccgca acatgtcatt aagcgcctct
     2821 acgaactcac aaaagatcgg gacccgatcg tgtcgaccga tgtcgggcag catcagatgt
     2881 gggcggccca atactttaag ttagcgaagc caaatcgctg gttgacctcg ggggggcttg
     2941 ggaccatggg ctttggattt cccgccgcca tgggggcgca agctgcgttt cccggtcggc
     3001 tggtcctttg catcgccggc gatggcagta tccagatgaa tatgcaggaa atggcgacgg
     3061 cggtggtgca taagctgccg gtcaagatta tcgtgctcaa caaccggttc cacggcatgg
     3121 ttaggcaatg gcaggatctc ttctacgagg gtcgatatgc ctcgagttat ctggagacga
     3181 ctcccgactt tgtgaagcta gcggaagcat atggatcggt cgggttcagg gccagcacgc
     3241 catctgacat cgatgcggtc ctcaaggaaa tgattgcggt ggacaaaccg gtgattgttg
     3301 atgtgcagac gtatccgtat gaaaatgtct atccgatgat tcccgccggc ggctgcaacc
     3361 atgagatgat tttggaagat ccgcctgctt tgaaaaacaa gcagcctgga gcggggcagg
     3421 tggctccgaa agattcagat acgatcctca cagcctaacc gacggtgcca ggagtcacgc
     3481 gaggtatgga acatattatt tcaatcaccg tggagaataa gtttggttcg ttgtcccgcg
     3541 tcgccggcct atttagcggt cggggattca atatcgaaag tctctctgtg gcgccgacgc
     3601 tcgatccgtc catgtcgcag atgaccattg tcacttctgg agacgaacgg atcatcgagc
     3661 agatcgtgaa acagctcaat aaacttatcg acgtcattaa ggttgtggat ctgaacgaga
     3721 gtgactttgt ctcgcgggaa acggcgctca tcaaagtgca tacgcggccg gaagatcgtg
     3781 cagaagcatt acggatcgcc gatattttcc gagcgaacgt cattgattcc acgccggcga
     3841 catacaccat cgaggtgacc ggcgatccgc ggaaaatcga agcgattatt aacctgctcc
     3901 aacccctcgg aatcaaggag ctcacacgta ccggtcgggt tgcagtcgct cgtgagacga
     3961 ttcggtcgac gggcgtgcag gcgaaaaaga tggcccgcga atagtctcta tagggcccat
     4021 cttcctcatt cgttcagtcg agcggacact attgttacct gggagtaatc gatgaaaatt
     4081 tactacgata aagatgccga tattcaacag atcaggaaca agaaggtggc ggtgatcggc
     4141 tacggcagtc agggtcacgc ccatgcgctc aacatgaaag agagcggtgt gagcgtggtc
     4201 attgggctgc gcgaaggcgc ttcatggaag aaggctgaac agagcggatt gaaggttatg
     4261 ccggtggctg atgccgtcaa gacgtctgac gtcgtcatga ttctcgcacc cgacgaggca
     4321 caagcggcta tctatcgaca ggatattgcc ccgaatctga agccgggttc ctctctcgca
     4381 tttggccacg gcttcaacat tcattttgga cagattgtac ctccggctac gatcaatgta
     4441 tttatggttg cgcccaaggg gcctggccat cttgttcgat cggagtatac gaagggcagc
     4501 ggggtgccgt gtttgctcgc tattcatcag gatccgagcg gaacaaccaa gcaagtaggc
     4561 ctcgcctatg ccagcgcgat cgggggcggg cgggccggtg tgatcgaaac aaactttcga
     4621 gaagaaaccg agacggatct tttcggcgaa caggttgttc tttgtggagg actcacgtcc
     4681 ctcatccagg ccggatacga gacgcttgtg gaggccggct actccccgga gatggcctat
     4741 tttgaatgcc tgcatgaagt aaaattgatc gtggatttga tctatcaggg cggcattgcc
     4801 aatatgcggt attctatcag tacaacggcc aagtacgggg atatcacccg tggtcctcgg
     4861 atcgtgacgg agcagacgaa gcaggaaatg aagaaaatct tgagtgaaat tcagcaaggc
     4921 cagtttgcca aagagtgggt actggagaat caggcgaacc gtccggtcta caacgcgttg
     4981 ctggctaaag gtgaagccca tcccatcgaa gaggtcgggg gccggctgcg ggcgatgatg
     5041 ccctggctga aaaaggacca attggtcgat aaaaataaaa actaagagtg aggactctgt
     5101 ggcagatcgt gccgtcggca ttccatttgc caaagaagga attcctttca tcggggctgc
     5161 tgtcggcgtt acactgctcg cagtatggct gggctggatc attgtggccg tcggggcggc
     5221 gatcctgacg ctcttcgttt cctggttctt tcgcaatccc tcccgggtgg tccctcaggg
     5281 gcctcatctc gttgtggcat ccggcgacgg gaaagtgatc gccatcgaag aagagttcga
     5341 gccgcgattt attaaggatc gcagcattcg cctcaccatc ttcttgaacg tgttcgatgt
     5401 gcacatcaat cggatcccct gtgaaggggt cgtcgaggag attcggtatc aaccgggtct
     5461 attccgcgtg gccagcaggc ctgatgcgac gttgcagaac gagcaaaacg ccctgatgat
     5521 ccggacagta gagggtggaa aggtgctgtg tgtgcaggtg gccgggttaa ttgccagacg
     5581 gattgtctgt tggatttcac cgggagatca ggctgtgcgg ggtgaacgat ttggcttgat
     5641 tcgatttggg tcgcggatgg acacatttct tcctatcggt acgactgtca aagttgctgt
     5701 cggcgatcgg gtcaaaggtg gggagaccat cttgggagag ttaccatgaa ggccaatgga
     5761 cttcgtcaat cgttcggaaa ggatgggaaa cggcgcaagg ccgccatgca cttgattccc
     5821 aatcttttta cgacagggaa tctcttgtgc ggcgtctttt cgatgctgtc agtattcaat
     5881 gcggactaca tggtcgcagc ggtcgcaatc ttggtggcga tggtctttga cgtgctcgat
     5941 ggaaagtcgg cgcgcctgac aaacagcacc agtcatttcg gcctcgaata tgactcgttg
     6001 tccgacgtgg tgtcgttcgg ggttgctcct ggcctcctga tttattcctg ggcgctgagc
     6061 ggccagggta cgtttggtat cgcagtgatg ttcgcatatg tggcgatggg tgcggttcgg
     6121 ttggcccggt ttaattcggc tgcagccgtg gctgacggca agtactttac gggattggcg
     6181 attcctgcgg cggccggggt cgtcgcgtcg cttgtgatat tcgaccatta tagtgtcagg
     6241 atcggaacgg aagtgaagcc catgctcgtt ctgattatta cgctgaccct ttcgttctta
     6301 atggtaagca ctatcaagta tcggagcttt aaagacatga agttccgcgg cggtcagcac
     6361 attacctatc ttgtctgggg aatcttgggg ctcatgctca tagccgcctg gcctcaagtt
     6421 atgctctttg tcatctttgc cggctacgcg ttgctcggtc cggcagaacg gcttgtcggg
     6481 cttctgttca agataaccgg aaagcgtccg tcgccgaaag ctgcgcagtc ggtgattgaa
     6541 tcgaagtgag agtggcatcg aagaggagta gtacgcgatt cgtacagcag tgagagagct
     6601 ggtgcgtggt gcaaaccagc ctgtgcggcg ccgaactcgc ctctgagcca gaattcgtga
     6661 aatccgagta atgaattccg cctcgccccc gtgacagggc gtcatgaagg gttgtggaca
     6721 gcgcacgttg ttcatggcca aatcagggtg gtaccacgga gcttttcaag cctccgtccc
     6781 tgggctgagg gatggaggct tttgtgtttt ttagattgct gaaaatgtct tcccgcggcg
     6841 ttctcggtcg cgtgcctccc tgcgacgtac ccatcggtga aagagctgtc tcggcagctc
     6901 ggggtgggtg ggtgagaaac ggtacgactc ggtcgtctcg cttcctgcgg ccttgcggag
     6961 aaggcatttt gagcaatctc ataagatcag agaggtgaaa tcatgacaag gatgatacgg
     7021 atttttgata cgaccctccg tgatggagaa caatcgccgg gggccagcat gaatgtggaa
     7081 gaaaaaatca tggtggcgaa gcagctggca cgcttagggg tcgatatcat cgaggctgga
     7141 tttgcctata gttcgccggg agattttact gcggtgcagc ggatcgcgca ggaagtcgag
     7201 ggaccgacga tttgcagctt ggcgcgggcg cgaccggagg acatcgaacg agcctgggag
     7261 gccttgaaag gggccccgaa ggtccgtatc catacgtttc tctcaacctc ggatatccac
     7321 ttgaagcatc
//
LOCUS       DATIPF010000204         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1453497, whole genome shotgun sequence.
ACCESSION   DATIPF010000204 DATIPF010000000
VERSION     DATIPF010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453497"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VLL06_05190"
     CDS             <1..283
                     /locus_tag="VLL06_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HSS30393.1"
                     /translation="AAPKKRSRSFSHTHSQGHCLELLKQLSAYIDDELPADICREIRR
                     HLGACPNCEVFIASLQHTVTLCRHRPAPQLTSVDRMNMRRAILDAAHAR"
     gene            280..681
                     /locus_tag="VLL06_05195"
     CDS             280..681
                     /locus_tag="VLL06_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HSS30394.1"
                     /translation="MSRTVGLICLALMLATPTHVLPTEIELESITIDIRGRMFIPNHI
                     LLHHDRQTALRFRNHDTELHTVAAKEFFQGVDFDLGGNGAPEYGPDGLKRVIIPPEGL
                     TEFRFTPARTGTFSYLCDMPGHDMKAVIVVE"
     gene            823..>2345
                     /locus_tag="VLL06_05200"
     CDS             823..>2345
                     /locus_tag="VLL06_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30395.1"
                     /translation="MMRLQIFQRSWATLLFLASTLSIVTAMTLTEPPSASAAAAKKIK
                     KAATPAEATAQRYAELMGAGNKVRVGQLDFACQYPLVVASLHGIKTYPSDSDPIYDSC
                     WQGLKDAHAPTLVRSDVGMEILWPSDGNLVFLSEDLNRYPASAFVTDSLGLRPPGTGF
                     QVHIVGSAPLPSASFRLRPNGPTVAVPTTLVKLSVSYQDPLTAPLTYAAGSVKWTSTI
                     KRARRGLKEITIQWVVLSGLKKHGFAGDTAVFNLPVTSGAVFGSGIQEKIPFATETSR
                     ALPDSLIWWGPTDTPGLLTAAVARAATFPDLRDRVAMLNRVLIIDPNQAEALMVLSRH
                     LYAIVLREAVPFHTLTVKDPALALVVNEHFWNIYAQTVRMDLSLGMEMGGFDKPTTAD
                     YLYRMLSAMQTLAVVRPDNLENRFRLGVAYRWNNDQEPAIATHQSLVKAIPAEQKAGR
                     AEALLQLAWSRINKVAWNRILDDSDIRAAYQDADEALTLADVPLDKFMAEYTKAYSL"
BASE COUNT          506 a          735 c          619 g          485 t
ORIGIN      
        1 cgcggcgcca aagaaacgtt ccaggtcctt ttctcacacg cacagccaag ggcattgcct
       61 agaacttctc aagcagttgt ctgcctatat cgacgacgaa ctcccagcag acatttgccg
      121 ggagatacgc cgacatctgg gagcctgccc aaattgtgaa gtcttcatcg cctccctcca
      181 gcacaccgtc acgctttgcc gacatcggcc agctccacag ttgacgtcag tcgaccggat
      241 gaacatgcgc cgcgcaatcc tcgatgcggc tcacgcacgg tgagcaggac cgtcgggctc
      301 atctgcctgg ccttgatgct cgcgactccc acacacgtac ttccaactga aatcgagctg
      361 gaatcgatca ccatagacat ccgcggacgg atgttcattc cgaatcatat cctcctacat
      421 catgatcgac agacagcact acggttccgc aatcacgata cggagctcca taccgtggca
      481 gccaaggaat tcttccaagg cgtcgacttc gacctgggcg ggaacggggc gccggagtat
      541 ggcccagatg gactcaaacg ggtaatcatc ccgcctgagg gcctgactga atttcggttc
      601 acaccggcac ggaccggaac cttctcgtac ctgtgcgaca tgccaggtca tgacatgaaa
      661 gccgtgatcg tggtcgaatg agaaggctga gcgattctgc tgcaatcgct gatccgcttt
      721 gacgacaagc cttcggccag cggactagcc tctttcagat gttcaaagca gcatgataga
      781 gttccgcctt cgtttcgtcc ttcatacgag gagcttgtcc gcatgatgcg cctacagatc
      841 ttccagcgta gctgggccac gctgctgttt ctcgccagca ccctgtcgat tgtcaccgca
      901 atgaccctga ccgagccccc gtcggcatca gccgctgctg cgaaaaagat caagaaagca
      961 gccactccag ctgaggcgac ggctcagcgt tacgccgaat tgatgggagc aggaaataag
     1021 gttcgcgtcg gccagctcga cttcgcctgt caatatcccc tcgtggttgc gtccctccat
     1081 ggcatcaaga cctatccgtc cgactccgac cccatctatg actcctgctg gcagggcctg
     1141 aaagacgcgc atgcgcccac gctggtccga agcgatgtgg ggatggaaat cttgtggccc
     1201 agtgacggca atcttgtttt tttgagtgaa gacctgaacc ggtatcccgc ctcggccttc
     1261 gtgaccgact cactgggttt aagaccgccg ggaaccggat tccaggtcca cattgtagga
     1321 agtgcaccgc tcccgtctgc ctcgttccgt cttcgaccga atggccccac cgtcgcggtg
     1381 ccgacgacct tggtgaaatt atccgtcagt taccaagatc ccctcacagc cccgttgacc
     1441 tatgcggccg gctcggtcaa atggaccagt acgattaagc gagccagacg gggactgaaa
     1501 gagatcacca tccagtgggt tgtcctttct ggactgaaga aacacggctt cgcgggcgac
     1561 acggcagtct tcaatcttcc tgtcaccagc ggtgcggtgt tcggcagcgg catccaggag
     1621 aagattccct ttgccacgga aaccagtcgg gctctccccg actcgctgat ctggtgggga
     1681 ccaacggaca ccccgggtct cttgaccgcc gcagttgcgc gcgcagccac atttccagac
     1741 ctgcgcgacc gcgtggccat gttgaatcgt gtactcatca tcgatcccaa tcaggccgag
     1801 gccctcatgg tactgagccg acacctctat gccattgtcc tgcgcgaggc cgtccccttc
     1861 cacaccctca cggtcaagga tccggcgctt gccttggtgg taaacgagca tttttggaat
     1921 atctatgccc aaacagtgcg catggacctg tcgctgggga tggagatggg tgggttcgac
     1981 aagccgacga ccgccgacta cctgtaccgc atgctctcag ctatgcaaac cttggccgtt
     2041 gtacggcccg acaaccttga aaaccgattt cggttaggtg tggcctatcg atggaataac
     2101 gatcaagaac cagcgattgc cacccatcaa tctttggtca aagccatccc ggccgagcaa
     2161 aaagccggca gggccgaagc cctgctccaa ctggcctggt cccgcattaa taaagtggcc
     2221 tggaaccgta tccttgacga ctcggacatt cgggccgctt atcaggatgc tgacgaagcg
     2281 ctcacgctgg ccgatgtgcc gctcgataaa ttcatggccg aatacaccaa agcctacagt
     2341 ttgca
//
LOCUS       DATIPF010000205         7137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_570945, whole genome shotgun sequence.
ACCESSION   DATIPF010000205 DATIPF010000000
VERSION     DATIPF010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7137
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570945"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VLL06_05205"
     CDS             <1..412
                     /locus_tag="VLL06_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS30396.1"
                     /translation="LGVVPAIRWYAIRQAERAGLQVHVEADSLPEDLPPDMAVVCFRI
                     VQEAVTNVLRHAKATQLDVVLRTTDSGFDLIVKDNGVGFRASDSMTEVEGRWAIGLVG
                     MRERARALKGHVKIHSAPGRGTEVCAHIPWEQVG"
     gene            445..1095
                     /locus_tag="VLL06_05210"
     CDS             445..1095
                     /locus_tag="VLL06_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSS30397.1"
                     /translation="MPIRVVLADDHILIRAGLRALLQSLPNIEVIAEASDGHEAVEVI
                     ARHQPDVVIMDIGMPGLNGVDSTRRIVKQFPGTRVVILSMHANEEYVGRALEAGAMGY
                     LLKGAEPSELEVVLKAVMRGETYLSPSIAKHLVQDYLSHRKEKTQALPDLTARQREVL
                     QLIAEGCSTKDIANKLKLSVKTIDTHRSELMNRLDIHEVAGLVRYAIRKGLVSTES"
     gene            1327..1698
                     /locus_tag="VLL06_05215"
     CDS             1327..1698
                     /locus_tag="VLL06_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30398.1"
                     /translation="MRFNRPIFGVLAMLPLLGALGLTGCSTVLPATLEAKLVSNATAE
                     DHLAAAMLYQNKARELEAEAMQFETAVSNVGSYADTKGFHRAALRSAAQEKRYGATQM
                     QQLYAQHLGQARALYGKTQPE"
     gene            1802..2170
                     /locus_tag="VLL06_05220"
     CDS             1802..2170
                     /locus_tag="VLL06_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30399.1"
                     /translation="MRSNKSLLRALTVLPLLELLSFTGCSGMMPATLQEKLAATGNEE
                     DHLAAAMLYQNKAQELEAEAVKYETAASKTGPSDSKGFQHAALLNAAQQKRADAKEMQ
                     EQYVTHFKQAQSLHGKAQPQ"
     gene            complement(2212..3078)
                     /locus_tag="VLL06_05225"
     CDS             complement(2212..3078)
                     /locus_tag="VLL06_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30400.1"
                     /translation="MLVIIALLLLPGCAITTPSPGRSTIIASLSYELPLSADVKSVVD
                     VLEASSARVLKEPVTVDSGVTTFLSGDAIHSVILLEKITALEGLGEVAIPSISCPLAL
                     TTIHKLITIEKGLRLVAGCVTVGASATRIDLLDMMTGHTSWPTDSTSSDDAATSSPIS
                     RIGADFVDRFPAIRLIGTPDIPIRRVNRGLDAETVTPTIPHIMAATTSGNMTTAAAKT
                     AALVCYAPTTTGVSVRDNPDSNTVVGTLDMELIVHEEDPSTYLFLHITTREGRAGWVK
                     RTDVRWIPCPIV"
     gene            3506..4033
                     /locus_tag="VLL06_05230"
     CDS             3506..4033
                     /locus_tag="VLL06_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30401.1"
                     /translation="MSRYCALMMVVLLSLSSGCSSSSTTQEKTILELNALVRNPNIQF
                     NLARFYHENQTTLVSIRYRNAGSSHAYRVVTDLKVFLDGHNVPIVEDSIGFVSTLAPK
                     GARELRGILPDAFLEKVMAGTMKLTMEFTAAYQNEQGELFSAVSIWQLSKTTMEPFLI
                     EDQIKLTAHPPAKVS"
     gene            4100..4537
                     /locus_tag="VLL06_05235"
     CDS             4100..4537
                     /locus_tag="VLL06_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HSS30402.1"
                     /translation="MATLNRRRSVRLSHFRLVLPETGRISVLGLLLATLLACDGSMQT
                     VSINAEDFRFTPDLMRVRASDPVTLSVYNAGREVHEFDSPILMYAAKTSLPKDTSQSA
                     STRGIEIRPGQSLQVVMAPSAGTYLYICRRKGHANMTGTLIVE"
     gene            complement(4621..5010)
                     /locus_tag="VLL06_05240"
     CDS             complement(4621..5010)
                     /locus_tag="VLL06_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30403.1"
                     /translation="MRRAVTIACFLMLMAAPVWAAEVEPFDPEAPFRQGLTSSLLRSL
                     LNEALDKFEDHIEFLGKLDSDDVKGNQQGRLQFKFYAEGKSKSDQHVGAEGWFRVAPD
                     GEQHDWHFKFTLPKDRKHAPLQFQEPL"
     gene            5394..5717
                     /locus_tag="VLL06_05245"
     CDS             5394..5717
                     /locus_tag="VLL06_05245"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HSS30404.1"
                     /translation="MVASRFDVFLVRLDPTQGREIRKTRPCLVISPDEMNHHIDTVIV
                     APMTTKGRPYPTRVQVRFKGKSGQIVLDQMRTVDKSRLVKRLGKIDEAARAHVLALLA
                     ELFAP"
     gene            complement(6505..>7137)
                     /locus_tag="VLL06_05250"
     CDS             complement(6505..>7137)
                     /locus_tag="VLL06_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30405.1"
                     /translation="RQQIYEVPTTEELFGQTASEGSALPLVPSHEDLEGAIPQEAISD
                     HVRMQWKLISLGVKASSRVWVPTGDQGRIRRLYNFDQFETDFAAGLDTQVRYVENIDV
                     VWKEEFRIDAAFEIENTTSIYSGLLRFSDLTLVAPNTIYPLFIVAPQDKRARLIEQLR
                     RPSFRRFHLERKVRYLSYEAVDEVDRFFENRNQGLSVDIITGRSEELKIA"
BASE COUNT         1706 a         1824 c         2015 g         1592 t
ORIGIN      
        1 tttgggggtg gtgcctgcga tacgctggta tgccattcgg caggccgaga gggcagggct
       61 ccaagtgcat gtcgaggcgg actcacttcc agaagatctg ccgcctgata tggcggtggt
      121 ctgtttccgg atcgtgcagg aagccgtcac gaacgtattg cgtcatgcca aagccacgca
      181 actcgacgtg gtattgcgaa cgaccgattc agggttcgat ctcatcgtca aggataatgg
      241 agtcggattt cgagcctcgg actcgatgac agaagttgaa ggccggtggg ctattgggct
      301 cgtcggtatg cgcgaacggg ctcgtgcgtt aaaagggcac gtcaagattc actccgctcc
      361 cggtagaggc acagaggtat gtgcacatat tccgtgggaa caggtcggtt aaccggattg
      421 ggataacgaa aagggatatg aacgatgcct attcgagtcg tgctggcgga cgatcatatc
      481 ctcatccggg ccggattgcg tgccttactg cagagtctgc ccaatatcga agtcatcgct
      541 gaggcgagtg atggtcatga agcggtcgaa gtcatcgccc gacaccagcc ggacgtggtc
      601 attatggata tcggcatgcc aggcttgaat ggggtggact ctacacgtcg aatcgtcaaa
      661 caatttcccg gcacccgggt ggtcattctg tccatgcatg cgaacgagga atatgtcggc
      721 cgggcgcttg aagcgggagc gatgggctat ctgctgaaag gagcggagcc ttcagaattg
      781 gaggtggtgc ttaaagcggt gatgcgaggc gaaacctatc tgagtccgag tatcgccaag
      841 cacttggttc aggattacct gtcccaccga aaagagaaga cgcaggcgct tcccgacttg
      901 accgcgcggc agcgtgaagt gctgcagctc attgccgaag gatgctcgac gaaagacatc
      961 gctaataagt taaagctgag cgtgaagacc attgacacgc atcgcagcga gctgatgaac
     1021 cggttggata ttcacgaggt ggccgggctc gttcgatacg ccatccgcaa aggccttgta
     1081 tcaaccgaat catgacgacg tctcacgagg agtgtcgtca acgctgagct ttcccccatc
     1141 ccgttccgcc ggtgtaaccc tcatccgtac gaagaccctc atctccttcc acagtccgat
     1201 cctcaggtat ttttcctgaa gttctccgag atcctcctga gacgaatccg tccatccatt
     1261 gtgtataggt ataggtaaaa gaacgtgacc tggctgtctt gagaagccta acaaggaggt
     1321 gtagcgatga ggttcaacag acccatattc ggagtactgg ccatgttgcc actgttgggg
     1381 gcgctcggtc tcacaggctg tagcacggtt ctgccggcga ccttggaagc gaaactggtg
     1441 tccaatgcta ccgccgagga tcacctcgcg gcagccatgt tgtatcagaa caaggcgcga
     1501 gagttggagg ccgaagccat gcagttcgag accgcagtct cgaacgttgg ttcctatgcg
     1561 gatacgaagg ggtttcatcg tgcagcattg cggagcgcag cgcaagaaaa gcgatacggc
     1621 gcgacacaga tgcagcagct gtatgctcaa catctcggtc aggcacgcgc cctatacgga
     1681 aagacgcaac ctgaataatg aaccaggcgg gccaaaagac aagcacgcgc aggagatgga
     1741 aatcatgaac gggaggtcgg tcaattggct gtcgtggaca gcccaacaag gagggggagc
     1801 gatgaggtcc aacaaatctc tattgcgtgc actcaccgta ttgccactgc tggagctgct
     1861 cagtttcacg ggctgtagcg ggatgatgcc cgcaaccttg caagagaaac tggcggctac
     1921 aggcaacgag gaggatcacc ttgcggcggc catgttgtat cagaacaagg cgcaagagtt
     1981 ggaggccgaa gctgtcaagt atgagaccgc ggcctcgaaa actggcccct ctgactctaa
     2041 ggggtttcag catgcggcat tgttgaacgc agcgcaacaa aagcgggctg atgcgaagga
     2101 gatgcaggag cagtatgtta cacactttaa acaggcgcag tcactgcacg gcaaggccca
     2161 gcctcaataa agaccttctg acgatgaggc tcgtggtgcc attccatcgt ctcaaacgat
     2221 gggacaggga atccatcgga cgtcggttcg cttcacccag ccggctcgtc cttctcttgt
     2281 tgtaatgtgc aggaacaagt atgtgctggg gtcttcctca tgcacgatca attccatgtc
     2341 caaggtaccg acgacggtat tgctatcagg attgtctcgc accgatacgc ctgtggtagt
     2401 cggtgcataa cagaccagcg ctgccgtttt ggctgcggcg gtcgtcatgt tgccggaagt
     2461 cgttgctgcc atgatgtgag gaatagtcgg cgtgacggtt tccgcatcga gacctcggtt
     2521 cactctgcga atggggatgt caggtgtgcc gatgagacgg attgccggga atcggtctac
     2581 gaagtcggcg ccaatgcgtg aaatcggaga agacgtcgcg gcgtcatcgg aactggtgga
     2641 atccgtcggc caagaggtgt gtccggtcat catatcgagt aggtcgatcc gagttgcgct
     2701 ggcgccgacg gtcacacagc ctgcaacgag ccgcaatcct ttctcgatcg tgatcagctt
     2761 gtgtatggtg gtcagcgcta gagggcatga gatcgatggg atggcgactt ctcccagtcc
     2821 ttcaagcgcc gtaatctttt cgaggagaat gacggagtgt atggcatccc cagaaaggaa
     2881 tgtagtcacc cctgaatcaa cggtgacagg ctccttgagg accctcgccg aagaggcttc
     2941 gaggacatcg acgacggatt tcacgtctgc agacagcggc agctcgtaag acagagatgc
     3001 gatgatggtc gagcgccccg gcgacggcgt tgtgatcgca catccgggaa gcaagaggag
     3061 tgcaatgatc acaagcatta cgtgtcggca cttgctgctg gtgtgcacag tatcctctcc
     3121 gagcacaaag atatgctcag ccgtggcagg ttgaaggact gtgtccggat attgagttgc
     3181 gccttcgacg gcaaggtgcc gttaccgctt tgagacacaa taaaaaaacg tccctgccca
     3241 gaagggagca aggacgccat tgtccgttca cacctgacga gagacgccgt tggctctcac
     3301 caaattttga aattattttt acagttcgct aagggagttg tcaaattctt caagagtagt
     3361 ctggcgttga aggggtggaa ttgaatcaag gtcagactgg ggatattccc agttgatgcg
     3421 ctcggatgac tcgcagagac tgagaggatt atttggcagg cgaccatgta gcgaaatggc
     3481 cacggtgggg taaggaagaa ccgagatgtc tcgatattgc gcgctgatga tggtggtctt
     3541 gctctccctg tcctctggct gtagcagttc atcaaccaca caggagaaaa ccattctgga
     3601 acttaacgca ctcgtgcgca accccaatat tcagtttaat cttgcaagat tctatcatga
     3661 gaaccaaaca acgctggtct cgatccgata taggaatgct ggttcgagcc acgcttatcg
     3721 tgtggttacc gacctgaaag tattcctgga tgggcacaat gtgccgatag tagaagattc
     3781 gatcggattt gtgtccacac tggctccgaa aggagctcga gagttgcgtg ggatccttcc
     3841 ggacgcattc ttagagaaag tgatggctgg cacgatgaaa ttaaccatgg aatttacagc
     3901 ggcatatcag aatgagcaag gtgaactgtt ttcggctgtt tcaatctggc aattaagtaa
     3961 gacgacgatg gagccatttc tcatagagga tcagatcaaa ctcactgctc acccgcccgc
     4021 caaggtatca tgagatcacg aggagtgttc cgtcggagag ctatagcggc cgtagggtta
     4081 ctggtcgacg tgaagagatg tggcgacctt gaatcgacgc cggagtgtca ggctgagtca
     4141 cttccgtttg gtcttgccgg agaccggtag gatctctgtc ctcggtctac tgctcgccac
     4201 ccttctcgcg tgcgatgggt cgatgcaaac cgtgtcgatc aacgcagaag actttcgatt
     4261 cacgccggac cttatgcgtg tgcgagcgtc ggatccggtc accctctccg tgtataacgc
     4321 cggacgggaa gtccacgagt tcgacagccc catcctcatg tatgcggcca agacgtcgct
     4381 gccgaaggac acgtctcaat cagccagcac ccgcggcatt gagattcgac ctgggcagtc
     4441 cctgcaagtc gtcatggctc cttcagcggg aacctatctc tatatctgcc gacgtaaagg
     4501 tcacgccaac atgaccggca cattaattgt cgagtaggtg ccttgtctca gtctgtacca
     4561 cgtaccgcac ggcacgtggc ctggggatgg atgtgtggca gaagcacaac cggagaggag
     4621 ttatagcggt tcctgaaact ggagcggggc gtgtttccga tctttgggca gcgtgaattt
     4681 gaaatgccaa tcgtgctgtt cgccgtccgg tgcgacgcgg aaccagcctt cagcgccgac
     4741 atgctggtct gatttggact tgccctccgc atagaacttg aactgcaacc gtccctgttg
     4801 attgcctttc acgtcatcgg aatcgagctt tcccaagaat tcgatgtggt cttcaaattt
     4861 atcgagtgct tcgttgagaa gtgaacggag cagacttgag gtcaatccct gccggaacgg
     4921 tgcctccggg tcgaacggct caacctcagc ggcccacacc ggagcggcca taagcatcaa
     4981 gaagcacgct atcgtaacag cccgtctcat ggtgattact ctattccccg aactctgcct
     5041 cgtcaagggc tcgccttagc tcaagtctct tgcgtttcgc acttgacatc cacgaggcta
     5101 cattgtaatt acactgtacc tacagcaagg gagggagaaa aatcgatgaa agcgcatgtc
     5161 gtgcgaattg gaaactccag gggaattaga attcccaagt ctgtgataga gcaatgtcag
     5221 ttgcacggag tagtcgatct catcgtacag caaggacaat tggttgttcg gtcagctgcc
     5281 aaagcacgag ccggttggga ccaggccttc gagcagatgc atcgccacgg agatgatcag
     5341 ttgctagatc ctgattcatt atcatcttcc gagtgggatc ggaaagactg gacatggtag
     5401 cttcccgatt cgacgtgttt ctcgtgcggc tcgatcctac ccagggtcgt gagattcgaa
     5461 aaacccgtcc ttgcctcgtc atatctccgg atgagatgaa tcatcacatc gacactgtga
     5521 ttgtggctcc catgacgacg aaagggcggc cctatcccac tcgtgtacaa gttcggttca
     5581 aaggaaagtc cggacaaatc gtcctggatc aaatgaggac cgtggacaag agccgattgg
     5641 tcaaacgtct gggtaaaatc gacgaagccg ctcgggctca cgtactcgct ctgctcgcgg
     5701 aactattcgc accgtagagg ccgggcgggc agtgcacgag ttctacaacc ccatcctcat
     5761 gtatgctgcc gataccgctc tagaccacat ggcagcgatg gatgaaaacg aaagtccggt
     5821 cgaggtatat gtcacgaaga actgatatta taacgtcgag ccggcgtgct acatcgagaa
     5881 cggaactggt ggtgtactaa attgcaggga acccgcttcc acatcataga cgaggaggtg
     5941 ccttacggac atatttgata agtcgagaca ggacatggac gccgaagtca agggatcggt
     6001 taaatgggtc gtgttagtcg ccatcgtcgg gctcgtgttg atgacgttgt acgtcatggt
     6061 cacataatcc cgccgaatgt caatcagcgt gtaccctggt tagcccacca gaaatccctg
     6121 actcgccaac attgccccaa cgcgcctgga tgggctccgt cacctggaag atctccgaaa
     6181 acagcagcag gtccggatac cgcccctcag tcgccggccg aaagaggtac gtgcgtatag
     6241 gcccagtgga agtgtggata tcaaccgact cggtatattt gataatcatc ccgttctccg
     6301 atgaggtgga tgaaattgaa ccccgaataa ggccggcaat gcagttgggg gaaaagtagg
     6361 cgcacgtcag gtcacttact ctgaagagtc actacaagat catttgcgat cagcagggac
     6421 tgttctagcg ctgctttgag gtcgtcagcc ttctcctcga caaacgagta tttcaacaat
     6481 ggctcctgac ttcataggtc tgacctatgc aatcttgagt tcttccgatc gacccgtgat
     6541 gatatctacg ctcagacctt gattgcggtt ttcaaaaaaa cgatcgactt catcaaccgc
     6601 ctcatatgaa agataacgaa ccttacgctc cagatgaaat ctcctgaaag acggccggcg
     6661 taactgctca atcagcctag cccgcttatc ttgcggtgcg acgataaaga gaggataaat
     6721 tgtgttaggg gcaacaagag tcagatcaga aaagcgtagc aacccggaat aaatcgaggt
     6781 ggtattttct atttcgaatg ccgcgtcgat tcggaactcc tccttccaca ccacatcgat
     6841 gttctctaca tatcgaactt gagtgtccag cccagcagcg aaatctgttt cgaattgatc
     6901 aaaattgtat agccttcgta ttctgccctg atctcccgta ggaacccaga ctcgactgct
     6961 cgccttcacg ccaaggctga taagtttcca ctgcatcctg acgtgatcag aaatcgcctc
     7021 ttgaggaata gcaccttcta agtcttcatg ggatggaaca agggggagtg ctgaaccttc
     7081 gctggctgtc tgtccgaata gctcttcagt ggtcggaact tcatagatct gttgtcg
//
LOCUS       DATIPF010000206         4618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1364833, whole genome shotgun sequence.
ACCESSION   DATIPF010000206 DATIPF010000000
VERSION     DATIPF010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364833"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1765)
                     /locus_tag="VLL06_05255"
     CDS             complement(<1..1765)
                     /locus_tag="VLL06_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS30406.1"
                     /translation="MNPKSLSRKDALRALPANQRERGMIIDPALDAILIMDDRGFITD
                     WNPQATELFGRSKSDMVGHSMPETIVPAPLREEFHRGLQRFGLDGEWGVLNRRVEIAA
                     LHQDGHEFPIELTIIPLLNEGVRTFSVFVRDLSGQKAAEYTLQRETSFVELLQRVAIA
                     ANEAQSVERALQFTLEQVCRYTGWPVGHVYLRDRAEEDLLQPTTLWHLNRPERFETFR
                     RITEGTRFPRGSGLPGRVLESSTAAWIPDVTKDVNFPRAQHAKDIGVKGGFAFPIVVG
                     GTVAGVLEFFSEQEAQPDVRLLEIMQVIGTQLGRVIERDKADQALRQAKELAETANRA
                     KSEFLANMSHEIRTPMNGILGMTQMMLQTPLNEQQRRYVDIAYRSGSGLLQIINDLLD
                     FSKIEAGKVELERIRFETRRPFEDVIDLFLERAKDKNLELACTMDAAVPAQLEGDPLR
                     LRQVVTNLVNNAIKFTERGKVVVGVCVAEQTADSCVLRCDVCDTGVGVAPSVRGSIFE
                     AFAQGDSSTTRKYGGTGLGLSIVKRLVDLMGGTVGVDSTVGQGATFWFTVRFGRIADT
                     PLAPLDEATRPPVQGAVCLSAQ"
     gene            complement(1860..>4618)
                     /locus_tag="VLL06_05260"
     CDS             complement(1860..>4618)
                     /locus_tag="VLL06_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS30407.1"
                     /translation="EPWFQAVRNGRKVHVGDVEPFAELGGVDSVAFTAPIAGPRGEFL
                     GVVTTRVGIPMLEDVLTRTLVTFEQREEFLGKMEYQFLTEKGVAFIGSDLQHKSHVNL
                     KQLGLPSALLSELTSSSYVEEEHRSRHVPVISGYAKTQGHGQFEGLHWTVLMRMDRRD
                     VLEPIRDMVWKVGLAGGAIVVPTFGLLLWTVRRVRREHRHAQQESMLAREAEASLRES
                     EAKVTTYAHELEQKNHNLDRAVAEAQDATQAKSSFLAVMSHEIRTPMNGIIGMTGLLL
                     DTAMTPEQRDYAETVRRSGEVLLNIINDILDFSKIEAGQLTFETIDFDLRTTVEEVLD
                     LFAESAQRKGLEMGCLLHAEVPTALRGDPGRLRQILVNLTGNALKFTQQGEVMIHVTR
                     GEEAAERVLIEFAVTDTGIGIAPEAQALLFKPFSQADASTTRKFGGTGLGLAICKQLV
                     EQMGGQIGIESIPGHGSTFRFTAWLTKQQAQAKPMPRGSLAGRRLCIVDDNATNRRIL
                     EEYASQWGLQSATALDGYQALALLKDAATRGEPFDLAILDMQMPRMDGLELARAITAN
                     RELAATRLVLLTSIGLRGQAEQAKQAGISAYLSKPVHRAHLYDCLSLIVDLPAKSAMD
                     PLEGGASSRPHDVLVTRHMLREAAGAARARILVAEDNTVNQKVAVCQLEKLGYRADVV
                     ATGLEAVEAVSRVSYALVLMDWQMPEMDGVEATAIIRKREEEQASRRLPIIAMTANAM
                     QGDRKKCLEAGMDDYIPKPVKLEHLKAVLARWLSGQPLSHEQKEPVSQEQHELVPECV
                     DSTVLTELRELDSSCGLLSTLITNFLNDVPTRLAGLQAALKNGDAGALARVAHELNGS
                     SGNLGIRHMRQLCVELQVLGKTKDLSKAGALLAQLVSEFELVRQRLMAEHATITDDTL
                     ADDT"
BASE COUNT          947 a         1461 c         1202 g         1008 t
ORIGIN      
        1 cctgcgcact cagacatacg gctccttgca ctggaggcct cgttgcctca tccagaggag
       61 ccaacggtgt gtcggcaatc ctgccaaacc gaaccgtaaa ccaaaacgtc gctccttggc
      121 ccaccgtgct atccacccca accgtccccc ccatgagatc gaccaatcgt ttgacgatcg
      181 aaagacccaa tcccgtcccg ccatattttc gtgtcgtcga gctatcacct tgtgcaaagg
      241 cctcgaaaat tgatcctcgg accgacggag cgactcccac accggtatcg cacacgtcac
      301 accgaagcac acaggaatct gcggtctgct ctgccacgca gacccctacg acgactttgc
      361 cgcgttcagt aaacttaatg gcattattca ccaggttggt cacgacttgc cgcagacgga
      421 gcgggtctcc ctcaagctgg gctggaacgg ctgcgtccat cgtacaggcc aactcgaggt
      481 ttttgtcctt cgcgcgctcc aggaagaggt caatcacatc ctcgaacggt cgccgcgtct
      541 cgaatcgaat gcgttccagc tccaccttcc cggcttcgat ctttgagaaa tccaagaggt
      601 cgttgataat ctggagcaac ccgctccctg atcgataggc aatatcgaca taacgacgct
      661 gctgttcgtt cagcggcgtc tgcaacatca tctgggtcat ccccaggatc ccgttcatcg
      721 gcgtccgaat ctcatggctc atattggcga gaaattcact cttggcccga ttcgcggttt
      781 ccgcaagctc cttggcctgc ctcaacgcct gatcagcctt gtcccgctcg atgacgcgcc
      841 caagctgggt cccgatcacc tgcatgattt ccaacagccg aacgtctggt tgggcctcct
      901 gctccgaaaa gaactccaac accccggcga ccgtccctcc caccacaatg ggaaaggcaa
      961 agccgccctt cactccaatg tccttcgcat gctgagcgcg tgggaagttc acgtctttcg
     1021 tgacatcagg aatccaggcc gccgtgcttg attccaggac gcgaccgggc aacccgctcc
     1081 cgcgaggaaa ccttgtgcct tccgtaatgc ggcgaaaggt ctcaaatcgc tcaggccgat
     1141 tgagatgcca gagcgtggta ggctgaagca agtcttcctc cgctctgtca cgaagataga
     1201 cgtgtcccac cggccagccg gtataccgac agacctgctc gagggtgaac tgcaacgcac
     1261 gctccacgga ctgcgcctca ttcgctgcga tcgccacgcg ctgcaacaat tcgacaaagc
     1321 tggtttcacg ctgcaaggtg tattccgctg ctttttgtcc agacagatct cgcacaaaca
     1381 cgctaaaagt acgcactcct tcgtttagca acgggataat agtcaactca atcggaaatt
     1441 catgaccgtc ctgatgaagg gctgcaatct cgactcgtcg attcaggact ccccattccc
     1501 cgtccaaacc aaaccgctgg agccctcggt gaaactcctc gcgtagtggc gccggaacga
     1561 ttgtctcagg catagaatgg cccaccatgt cggatttgga ccgcccgaac agctcggtgg
     1621 cttgcggatt ccagtcggtg atgaatcccc gatcgtccat gatcaggatc gcgtccaagg
     1681 caggatcgat gatcatgccc cgttcacgtt ggttcgcagg cagggcgcga agggcgtctt
     1741 ttcgactcaa tgatttagga ttcatcgtat gtgcacgcga agtatccggc aaatgtgggt
     1801 ttcaacactt tcatcgacgc tcagaatggt cttttgatcc atatgcatgt ctcgccttct
     1861 tatgtgtcat cggcaagcgt gtcatctgtg atcgtcgcat gctcagccat cagacgctgg
     1921 cgcaccaact cgaattcact caccagctgt gcgagtagcg ccccagcctt gctgaggtcc
     1981 ttggttttcc caagcacttg gagttcgaca cagagctggc gcatgtgccg tatacccagg
     2041 ttgccgctgg agccattcaa ctcgtgcgcg actcgcgcca atgctcctgc atcaccattc
     2101 ttgagcgcgg cctgtaagcc tgctagacgg gtgggaacgt cattcaaaaa atttgtgatc
     2161 agcgtggaca ggaggccaca ggagctatcg agttcgcgta attcggttaa tactgtggag
     2221 tctacacact cagggaccaa ttcgtgttgt tcctgcgaca ccggttcctt ttgctcatga
     2281 gacagtggct ggccggacaa ccatcgggcg agcaccgcct tgaggtgctc cagcttcacc
     2341 ggcttgggga tataatcgtc catgcctgcc tcgagacatt tcttccggtc cccttgcatg
     2401 gcgtttgcgg tcatggcgat gatgggaagt cgtcggcttg cctgttcctc ttcgcgcttc
     2461 cgaatgatcg ccgtcgcctc cactccatcc atctcgggca tttgccaatc catcaacacc
     2521 aacgcgtaac tcacccgaga gactgcttcg accgcttcga gtccggtggc tacgacatcg
     2581 gcccgataac ccagcttttc cagctgacac accgcaactt tctggttcac ggtattatcc
     2641 tcggccacaa gaatacgggc acgcgccgcc cctgcagctt ccctgagcat gtgacgtgtc
     2701 accaatacat cgtgaggtcg actcgaagcc ccaccctcca gcgggtccat ggctgacttc
     2761 gcaggcaagt ccacaatcag tgacagacag tcgtatagat gtgcgcgatg gacgggtttg
     2821 ctcagatacg cggagatccc cgcttgtttc gcctgttcgg cctgcccccg cagaccgatt
     2881 gaggtcagca agacaagacg ggtcgccgcc agctcgcgat tcgccgtgat cgcacgcgcc
     2941 aactccagtc cgtccatgcg gggcatctgc atatcgagga tcgccaaatc gaagggctct
     3001 cccctggtcg ccgcgtcctt cagcagcgcc agggcttggt agccgtccaa tgcagtagcg
     3061 ctctggaggc cccactgtga tgcgtactct tccaggatgc gtcgattggt ggcattgtca
     3121 tccacaatgc acaatcgacg acctgcgaga gaccctctcg gcatgggttt cgcctgagcc
     3181 tgttgtttgg tcagccacgc cgtaaatcgg aaggtgctgc cgtgaccggg aatactctcg
     3241 atgccgatct gcccgcccat ctgctcaacg agctgtttgc aaatggcgag gcctaggccg
     3301 gtgccgccga atttccgcgt ggtcgaggca tctgcctggg aaaagggctt gaacaaaagc
     3361 gcctgtgcct cgggggcaat gccgatcccc gtatccgtca cagcaaattc gattagaacg
     3421 cgctcggcag cctcctcgcc tcgagtcaca tgaatcatca cctcgccctg ctgggtaaac
     3481 ttgagtgcat tgccggtgag attgacgagg atttgacgca agcggccagg atcgcctcgt
     3541 aacgccgtgg gcacttcagc atgcaagaga caacccattt ccaggccctt gcgctgcgcc
     3601 gactcggcaa aaaggtccaa gacctcttcg accgtcgtcc gaaggtcaaa atcgatcgtt
     3661 tcgaacgtca actggccggc ctcgatcttc gagaagtcca ggatgtcatt gatgatgttc
     3721 agcaggactt caccggatcg acgcacggtc tcggcgtagt cgcgctgctc aggcgtcatg
     3781 gcggtatcga gcaagagccc ggtcatcccg atgatgccgt tcatgggggt ccgaatttca
     3841 tggctcatca cggcaagaaa ggaggacttg gcttgcgtgg cgtcctgcgc ttcggccacc
     3901 gcccggtcga ggttatggtt tttctgctca agttcatgcg cataggtggt aactttggcc
     3961 tcgctttccc gtagcgatgc ttcggcttcc ctggcgagca tactctcctg ctgcgcgtgc
     4021 cgatgttctc tccgcacgcg tctcacggtc catagcaaga gtccaaatgt tggaaccact
     4081 atggcgccgc ccgccaggcc aaccttccaa accatgtctc gaatcggctc cagcacatcg
     4141 cgccgatcca tgcgcatcag tactgtccaa tgcaaaccct caaactggcc atggccttgg
     4201 gtcttggcat agccgctgat gactggaaca tggcgacttc tgtgctcctc ttcgacgtaa
     4261 ctggatgagg tcagttcgct caacagcgcc gacgggaggc cgagttgttt cagattgaca
     4321 tggctcttat gttggaggtc cgaaccaatg aaggccaccc ccttctcagt caggaactgg
     4381 tactccatct ttcccaaaaa ctcctcccgc tgctcgaaag tcacaagggt ccgggtcagg
     4441 acgtcttcca gcatcggaat ccccacccgc gtcgtcacga ctcccaggaa ctccccacgc
     4501 ggtccggcga taggagccgt aaaggctacg gagtccacac cgcctagctc tgcgaatggt
     4561 tccacgtccc ccacatgaac ttttcgcccg ttacgcacgg cctgaaacca gggttcac
//
LOCUS       DATIPF010000207         4975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1190614, whole genome shotgun sequence.
ACCESSION   DATIPF010000207 DATIPF010000000
VERSION     DATIPF010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190614"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VLL06_05265"
     CDS             <1..285
                     /locus_tag="VLL06_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSS30408.1"
                     /translation="RSVLAERFRRYLSETPIAYLTRWRLQLGAQLLTSTSNSVAQIAA
                     EVGYESEPSFNRAFKREFSLPPARFRSKSKSTRSIATRHPSTNSRRSAKR"
     gene            362..628
                     /locus_tag="VLL06_05270"
     CDS             362..628
                     /locus_tag="VLL06_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30409.1"
                     /translation="MQDAHFVEGLELVSCVIGVAYGAFLIYGIQQQWRWITDPPEWTS
                     VIYFPTVVKMIWGPKHVRFFAYLTAYGSLVMSLICLVQSYLDRP"
     gene            785..970
                     /locus_tag="VLL06_05275"
     CDS             785..970
                     /locus_tag="VLL06_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30410.1"
                     /translation="MRFVKVQDEERSGQVAINLDLVREAHFGGGLLHLYFERNATSQD
                     DVTFTGDNAAKLWAAMG"
     gene            1183..1824
                     /locus_tag="VLL06_05280"
     CDS             1183..1824
                     /locus_tag="VLL06_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HSS30411.1"
                     /translation="MLTAIGDVMRRVYERGWITTRDGNISMRKREGKYLYITPSGWRK
                     TIVHPEHVVRIEIVKEPLTGELIPNVHEGQRPSGELWMHWNLQRDTKKTRTVVHVHAT
                     HIVAAMYAGIDLQVISAEFPEISRYTRVGPTVPALPALSRELADATADCFGLGKNGVL
                     TFDIVGQANHGVCAVATDPWAAYEHIERLDHICEIVLKSGVNRSLPVSRSVAT"
     gene            complement(1946..2248)
                     /locus_tag="VLL06_05285"
     CDS             complement(1946..2248)
                     /locus_tag="VLL06_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HSS30412.1"
                     /translation="MVRVGLLVRLQAKPGKEADVARFLESGLALANQEAATQVWFALK
                     LGPATFGIFDAFADDAGRKAHLGGQIAAALMAKAPELLAEAPKIEQVDVLAAKISH"
     gene            2364..3314
                     /locus_tag="VLL06_05290"
     CDS             2364..3314
                     /locus_tag="VLL06_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSS30413.1"
                     /translation="MLALNDVFDTGLSTLLDTFSTANELAESAGTASARFDVTVIGVR
                     PRVHTNQGLSVPIRPAMRLARPDVVLVPALGAKMPEPLRAVLQRRDIIDAQALLRRWS
                     GSGTLVGAACTGTFVLAGTSLLDGQRATTSWWLAPFFRERYPHVILDESRMLVGSSRF
                     VTAGAALAHLDLALWLVRRRSPALAALTARYLVVDPRSSQAAFSIPDHLAHSDPLVER
                     FERWARRQLADGFSLSEAARAALTSERTLARRLQSVLGKSPLAFFQDLRVERAVHLLQ
                     TTSASVDQIADQVGYADGVTLRTLIRRKLGRGVRELRVRG"
     gene            complement(3386..4279)
                     /locus_tag="VLL06_05295"
     CDS             complement(3386..4279)
                     /locus_tag="VLL06_05295"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSS30414.1"
                     /translation="MAILLIDDSQDDCLLVGAYLKSANYVVVSKNSAKEALDYLEGLI
                     EGKPLTPIDLILLDVKMPGFDGLDACTRIKSMKPFEGVPILMVSADTTPGTIQLAFRR
                     GAVDYIRKPIIKAELLAKVAMVSRIQEDTNHRVQSEKMASQKANIQVSPTIDNVTGIM
                     NWWSFDELFELEWGRAAQEHLPISLLFFSVGNFKAFNDTYGYATGDECLRRIAQAAQE
                     ALAQAGQVVARYRGAEFVALLFGTGDEDAKSVLHHLHETIEALDLGPIVDVGVATAYP
                     TESNSRADLLTSAKNAVPNRK"
     gene            4553..4843
                     /locus_tag="VLL06_05300"
     CDS             4553..4843
                     /locus_tag="VLL06_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30415.1"
                     /translation="MIVFRTALKEPVHELLKKSGVKAYTEVPQTAGMGQSGSTEGGSF
                     YAGGNTVILASLEPAQRDKVAGAITAWRTEAQQAGWVKPTIRVFSWPCTQLI"
BASE COUNT         1089 a         1425 c         1340 g         1121 t
ORIGIN      
        1 cgctccgtac tggccgagcg gttccggcgt tatctctcgg agacccccat tgcctacctc
       61 acacgttgga ggcttcaact cggagcgcag ctgctcacat ccacgagcaa tagcgtcgca
      121 cagatcgcgg ctgaggtcgg ctatgaatcg gaaccatcgt ttaatcgtgc cttcaaacgt
      181 gagttcagtc tcccgccggc tcgatttcgc agcaaatcaa aatcaacccg gagcatcgcc
      241 acccgccacc catcgacgaa tagccgccgc agtgcgaaac gatgagcgaa gcggtgccac
      301 tgacgcagga tgggacgctg gttgagtaga atgacctggt tcatcctgtg tgggagcagg
      361 aatgcaagac gctcacttcg tggaaggttt ggaacttgtt tcctgtgtga tcggggtcgc
      421 ctatggcgcg ttcttgatct atgggatcca acagcaatgg cgctggatca cggatccacc
      481 ggagtggacg tcggtgatct actttcccac cgtcgtcaag atgatttggg ggcccaagca
      541 cgttcgtttc ttcgcctatc tgactgccta cggttctctt gtgatgagtc tcatttgcct
      601 cgtgcaatcc tatcttgata ggccctaaga gactccccca ccaaccggcc tcatcaacca
      661 tttccatccg tctattcttg acacaccacc cgtcgccgca taggttatgc cgacgtttac
      721 caggcatggg actcttcgtc ccatacgaac ttccatcgtc acatcacaca aggaggcagg
      781 cgttatgcgg ttcgtgaagg tacaagacga agaacgatcc ggtcaggtcg ccatcaatct
      841 tgaccttgtc cgagaagcgc attttggagg gggactcctg cacctgtatt ttgaacggaa
      901 cgccacctca caggatgacg tgacctttac cggtgataat gctgccaagc tttgggctgc
      961 aatgggctaa gcgaatgtgg cgaccgtgcg gcgaaggaat gctcatcgag taacagcgtg
     1021 ggccgattcg ttcgctgccg gccggccccg ccgattaccc tctctgcagg accacagtaa
     1081 ctgaaacctg tcgactcgaa tcgttcggct accagccctc atgcaatagg gaacgaacac
     1141 tgttatgcta tcttagaaga gacacacact taaggagcga acatgctgac agcgattggc
     1201 gatgtcatga ggcgcgtgta tgaacggggc tggattacga cgagagatgg aaacattagt
     1261 atgcggaaac gagaggggaa gtatctgtac attacccctt ccggatggcg gaagactatt
     1321 gtgcatccgg aacatgtcgt acggatcgag attgtgaagg agccgctgac aggcgaactg
     1381 ataccaaacg tccatgaagg gcaacgaccg tccggggagc tctggatgca ctggaacttg
     1441 cagcgcgata caaaaaagac ccgaacggtc gtgcatgtcc acgccacaca catcgtcgcc
     1501 gccatgtatg caggaatcga cttgcaggtc atcagcgcag agtttcctga aatctcgcgc
     1561 tacacccgcg tggggccgac ggtgcccgca ttgccagcct tgtcacgtga attggcggac
     1621 gcgacggctg attgtttcgg tcttgggaag aatggggttc tcacattcga tatcgtgggg
     1681 caggcgaacc atggtgtctg cgcagtcgcg acagacccat gggccgccta tgaacatatc
     1741 gagcgcctcg atcacatctg cgagattgtc ctcaaaagcg gtgtgaacag gtctctgcct
     1801 gtcagtagat ctgtcgcgac ctaagagacc taccctgact tcccaaacaa ggtcttctct
     1861 ggccagtcgg acgacatacg gaacatactc ccacgcgcct ggtgcgaccg agtcgactct
     1921 accaactttc cactgccgaa tcgattcagt gtgagatttt cgccgcgagc acgtcgacct
     1981 gttcgatctt tggtgcttcg gcaagcaact cgggcgcctt ggccatgagg gctgccgcaa
     2041 tctggccgcc cagatgcgcc ttgcggcctg cgtcatcggc aaaggcatcg aaaataccga
     2101 acgttgccgg tcccaacttc agcgcaaacc agacctgagt ggcggcttcc tgattcgcga
     2161 gggcgagccc actttcaaga aagcgagcga catccgcttc cttccccggc ttcgcttgta
     2221 atcgaactaa caacccaact cgtaccatgg tagtccatcc tttctaaaat tgaagaacat
     2281 gagggaaatt ctagtggtga acggcaatct gcgctagtgg caggaatgac aattatgata
     2341 cagttcttgc catgcgtatt tacatgctcg cactcaatga cgtcttcgac accggcttat
     2401 ccactcttct cgacacgttc agcacggcga acgaactggc tgaatccgcc ggaaccgctt
     2461 cggcgcgttt cgacgtcacg gtcatcggtg tccggcctcg cgtgcacacg aatcaagggc
     2521 tttcggtacc gatacgaccg gcgatgcgac ttgcgcgtcc ggatgtcgtg ctcgtaccgg
     2581 cgctcggagc gaagatgcct gaacccctgc gagctgtgct tcaacggcga gatatcatcg
     2641 atgcgcaggc gcttctgcgc cgctggtccg gcagcggcac gctggtgggt gccgcctgca
     2701 caggaacgtt tgttctcgcc ggcacgtcat tgctcgacgg acaacgtgca accacgtcgt
     2761 ggtggcttgc cccattcttt cgtgaacggt acccgcatgt cattctagac gaatcgcgta
     2821 tgttggtggg ttcatcacgc ttcgtgaccg ctggtgccgc tctcgcgcac ctcgatctcg
     2881 ccttgtggct ggtacgtcgc cgtagcccgg cgctggccgc actgacagcc cgctatttgg
     2941 tcgtcgaccc acggtcatcg caggcggcat tttcgattcc agaccacctt gcacactcag
     3001 acccgctcgt cgaacgcttc gaacggtggg cccggcgcca actggccgac ggcttctcgc
     3061 tcagtgaagc ggctcgcgca gccctgacaa gcgaacggac cctggcgcgt cgcctgcaat
     3121 ccgtgctggg caaatccccg ctcgcattct ttcaggatct tcgcgtcgaa cgcgcggtcc
     3181 acctgttgca aacgaccagc gcaagcgttg accagatcgc cgatcaagtc ggctatgccg
     3241 atggggtgac tctgcgcaca ctcatccggc gcaagcttgg ccgtggcgtt cgcgagctac
     3301 gtgtacgagg ttgatgaggt tgggacgtga tatgggatcg gcttcttcta ttctgaaccg
     3361 cgatcaggat aggattgcgt gacttctact tccgattggg aactgcattc ttggccgacg
     3421 tgagaaggtc cgcacgagaa ttgctttcag taggatacgc cgtcgccacc ccgacatcaa
     3481 cgatgggacc gaggtccaag gcctcgatgg tttcgtgaag atgatgtaac acggacttcg
     3541 catcctcgtc tcctgtgcca aataacaacg cgacaaattc cgcaccccga taccgtgcca
     3601 caacctggcc tgcctgagcc aacgcctctt gtgcagcctg ggctattctc cgcagacact
     3661 cgtcacccgt cgcatatcca taggtgtcgt taaaggcctt aaaatttccc acgctaaaga
     3721 agaggagtga aataggaaga tgttcttgtg ccgcacgtcc ccactccagc tcaaagagct
     3781 catcgaagct ccaccagttc ataatgcccg tgacgttgtc gatcgttggg gagacttgga
     3841 tgttggcctt ctgggatgcc atcttttctg actgcactcg atggttagtg tcttcttgaa
     3901 tcctcgatac cattgcgacc ttggctagga gttcggcctt gatgattggt tttcgtatat
     3961 agtcgacagc cccacgccga aacgccaatt gaatggtacc gggggtcgta tcggccgaga
     4021 ccatgaggat tgggacccct tcaaacggtt tcatggattt aatccgcgtg caggcatcca
     4081 gtccatcgaa cccaggcatc ttgacatcga gcagaattag atcgattggt gtcaatggct
     4141 tgccctcaat caggccttcc aaataatcca acgcctcttt tgccgaattc ttggacacga
     4201 ctacgtagtt tgccgacttt aggtaggccc cgacgaggag acaatcatcc tgtgaatcat
     4261 caatgagtaa gatggccata gatcgctttc aagatcaatg cgtttaccct accaggaaag
     4321 agccagccga ccaaggcatt cttaagaaag aggattgatt gtaagaagaa ggagatgcaa
     4381 cctccggaga cgaactgctg tttttctcac tagccaattc tctgaatttt ccaccggcag
     4441 agaaacgggc ctgctattgt ttctcttatg actctccccg catctatcaa gatcgatccg
     4501 gaagctgcta tattattttc tcaccttctt cgaggtacgc atggacatgt tgatgattgt
     4561 gtttcgtact gcactgaaag aacccgtgca tgagttattg aagaagtccg gggtcaaggc
     4621 ctacaccgaa gttccacaga cggcagggat gggacaatcg ggatccacgg aagggggttc
     4681 gttctatgct ggaggaaaca cggtcatcct cgcctccctc gaaccagctc aacgtgacaa
     4741 ggtcgccggc gcgatcacgg cttggcgtac agaggcgcag caggcaggat gggtcaaacc
     4801 gaccattcgg gtattttcct ggccctgtac acaactcatt tagtcgctcc tattctcgag
     4861 acggctcggc ggatgggggc tttcgcctca atttgttgta gaatatttca gtcgtgaatc
     4921 gcctctccga ggttcacatg gcacggcgca cccaggctca actacagcag accta
//
LOCUS       DATIPF010000208         2726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1543855, whole genome shotgun sequence.
ACCESSION   DATIPF010000208 DATIPF010000000
VERSION     DATIPF010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543855"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VLL06_05305"
     CDS             <1..352
                     /locus_tag="VLL06_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HSS30416.1"
                     /translation="GPAHHIVALGTNRVAASAMKQAGATQVGVGACAMADTLPTVDVI
                     VTSLNLVLPGSMLGEVTPEIVHAILGARATKVLLPLNRARVEIVGARAYTLEALITQS
                     LSRVRSLVSSVQPA"
     gene            477..2522
                     /locus_tag="VLL06_05310"
     CDS             477..2522
                     /locus_tag="VLL06_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HSS30417.1"
                     /translation="MRYLVLVLACLTAWLIIVEPSQAHDPDQDILEIPEVRVSSDKPV
                     AASSQQFIPDKEILLQPQGRPAQVLRLIPGMIAVEHSGGAGKADQYFLRGFDADHGTD
                     VALFADGMPINLRTHAHGQGYADLNFIIPETIEGLDVNKAAYLPEYGDFATAGAINFK
                     SRQVVQEGMAQATGGQFDTQRYLLMFSPTKDKVRTLFAAEGYYTNGPYEHENRYFRGN
                     LFGKLTTNLSSRDELSVTGTFQSSKWNASGEIPLRAVEAGTLGRFGSIDPSEGGNTLR
                     STARLNYHYDTVSGGQFFANAYGQYYRLDLFTNFTYFLNDPVNGDGIQQSDRRVMYGG
                     DLGYKQQGEVLGMPSIGTIGFQTRVDDIHLRLGTQTTRTPTGTTTDNDVLEASYAPFI
                     KAEVQPMPWMRLTGGLRGETFTFDVHNRCPTCAEQANGRKSSGIILPKINLILGPWAG
                     TELFVNYGEGFHSNDARSAVTPGSSPLARSKSYEVGVRSKPWGPEGIELIATAWLLDL
                     QSELVFVGDEGTTEIRGKSRRQGLEGAMRGQVWGPIYVNGSVTWTKAEFRNGDAIPLA
                     PELTAYGAVLVRWPEGLASQLQATYLGVRPLTEDRSIKSPSWVEVDLTERYQLPITLS
                     HGRLEAFFFIRNLFDTKWEQAIFAFESRLRNETAGVTDIHFVPGNPRMFLGGVAWYF"
BASE COUNT          568 a          747 c          812 g          599 t
ORIGIN      
        1 tggccccgcc catcacattg tggcgttggg gaccaaccgt gtcgcggcgt cagcaatgaa
       61 acaggccggc gcgacgcagg ttggtgtcgg agcatgtgcg atggccgata cgttgccgac
      121 ggtggacgtg attgtgacct cgttgaattt ggtgttacct ggctcgatgc tcggtgaggt
      181 gacaccggag atcgtgcatg ccattctcgg cgcacgggcc acgaaggtcc tgctgccgct
      241 caatcgggct cgcgtggaaa ttgttggcgc aagagcgtat acactcgagg cattgattac
      301 gcaatccctg tcccgagtcc gttctttagt gagttcggtc cagccagcct aggcgattcc
      361 tccaagtcat cgttgttgcc aggccacgag ggtctgcgcc agtcgatgag tcgactgtcg
      421 aacgatcgtc gtgggcgtct catccgccca tgcctctgtg tgtcagggat gagaccatgc
      481 gataccttgt gctcgtgctg gcctgtttga ctgcgtggtt gatcatcgtt gagccgtcac
      541 aggcccatga tccggatcag gatatcctcg aaattcccga agtcagagtc agcagtgaca
      601 aaccggtggc ggcttcatcc cagcagttca tcccggacaa agaaattctg cttcaaccgc
      661 agggacgccc ggctcaggtg ctgcgtctca tacccggcat gatcgcggtc gagcattccg
      721 gtggggcggg gaaggccgat caatattttc tgcgtggctt cgatgccgat catggaaccg
      781 acgtggctct gtttgccgac ggaatgccga tcaacctgcg cacccatgcg catgggcagg
      841 gctacgcgga cctcaatttc atcattccgg aaaccatcga ggggttagat gtcaacaaag
      901 ccgcctatct cccagaatat ggtgactttg ccaccgccgg ggcgatcaat ttcaagtctc
      961 gacaggtggt gcaagaaggg atggcgcagg cgaccggggg gcagtttgat acgcaacgct
     1021 atctgctcat gttctcaccg acgaaggaca aagtgcggac gttgtttgct gcagaaggct
     1081 attacaccaa tggtccatat gaacatgaga accgctattt ccgaggcaat ctgttcggca
     1141 aactgacgac caatctgagc agtcgagatg aactcagtgt gaccgggacg tttcaaagct
     1201 cgaaatggaa tgcctccggt gagattccac ttcgtgccgt cgaggccggc acactcggtc
     1261 gattcgggtc catcgatcct tctgagggag gcaacacgct ccgcagcacg gcccggttga
     1321 actatcacta tgacaccgtc tcaggcggcc aattctttgc gaatgcgtac ggccagtatt
     1381 accggctgga cctcttcacc aactttacat attttctcaa cgatccggtc aacggcgacg
     1441 gcattcaaca atccgatcgg cgggtgatgt atggcggaga tcttggatac aaacagcagg
     1501 gtgaagtcct gggcatgccg agcatcggga cgatcggatt tcaaaccaga gtcgacgaca
     1561 tccacttgag actggggacg caaacgacgc gcacgccgac cgggaccacc acagacaacg
     1621 atgtgctgga ggcgtcctat gcgcccttca tcaaggcgga ggtccagccg atgccatgga
     1681 tgaggctgac aggtgggctg cgcggcgaaa ccttcacctt cgacgtgcat aaccgctgtc
     1741 ccacctgtgc ggagcaagcg aacggcagaa agagttccgg catcatcctg ccgaaaatca
     1801 acctgattct cggtccctgg gccggcacag agctctttgt caattatggg gaggggtttc
     1861 atagcaatga tgcgcgctcg gccgtcacgc ctggttcgtc gcccttggcc cggtccaaga
     1921 gttatgaagt cggggtccgg tcgaagccct gggggccaga ggggatcgaa ttgatcgcca
     1981 cggcttggct cttggatctc caatcggaac tcgtgttcgt gggggacgaa ggaaccacgg
     2041 agattcgcgg aaaaagcaga cggcagggtc ttgaaggcgc catgcgtggt caagtgtggg
     2101 gcccgattta tgtcaacggc agcgtgacct ggacgaaggc tgaatttcgc aacggcgatg
     2161 cgattccctt agcccctgaa ctgacggcgt atggcgcggt gttggtccgt tggcccgagg
     2221 gcctggcctc acagctgcaa gcgacctatc tcggcgtacg cccactgacc gaggaccgga
     2281 gcatcaaatc gccctcatgg gtggaagtcg acctcacaga gcgctatcaa ctgccgatca
     2341 cgctgtccca cggacggttg gaggcgttct tctttattcg gaatctgttc gacacgaagt
     2401 gggaacaggc catttttgcc tttgaatcga gattgcggaa cgagacggcc ggtgtcaccg
     2461 acattcactt cgtgccggga aacccgcgta tgttcctggg cggcgtggcc tggtattttt
     2521 gacaccacac cactcaatct ggcaaattgt gcgtgctctc tgtcatcagc gtcgaacagt
     2581 cttccctttt tcttccaggg aatgaatatg tttgtacgga gtgttgccgc gactgacgac
     2641 cgggttgtgt ttaatcggaa taggagtggg gtgctgtatg ccgattactt ctgtatcgca
     2701 gtttattgat catcactatc gacact
//
LOCUS       DATIPF010000209         4192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1273161, whole genome shotgun sequence.
ACCESSION   DATIPF010000209 DATIPF010000000
VERSION     DATIPF010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273161"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VLL06_05315"
     CDS             <1..529
                     /locus_tag="VLL06_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HSS30418.1"
                     /translation="IQLANAEVSGQPVFAGTETGAPPFVLGAGDAVTYQGNSESQSIA
                     VGPNQTTQILLPGDQVFAGATTNIFTDLSNLLVALETNSGAGIEVGIGNLDRAVDQLS
                     VAQGQIGAIANRLDTAHGSLLVVSAAIQQALSDQTDTDMATVLTELQMQQTAYQATSQ
                     TFSRLFDSSLLNFLR"
     gene            603..839
                     /gene="csrA"
                     /locus_tag="VLL06_05320"
     CDS             603..839
                     /gene="csrA"
                     /locus_tag="VLL06_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HSS30419.1"
                     /translation="MLVLTRRRGEGVAIGPDIRIVVLGIKNGQVRLGIEAPRTIEVHR
                     DEVCVRIQEENQLAARTPMIPLEVFQRLLRTKIA"
     gene            858..1349
                     /gene="fliW"
                     /locus_tag="VLL06_05325"
     CDS             858..1349
                     /gene="fliW"
                     /locus_tag="VLL06_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HSS30420.1"
                     /translation="MHITSTRFGVLNVPDETLLSFPSGLLGFPEWTRYVVLDHDTDAP
                     FKWLHCAEEGSLAFVVIDPALFNENYHVTISPEARGEVEGNEADELGLAVILTIPSDD
                     PSAVTANLRGPLLLNPRTRLCKQLVLSEDYPTRYPIFPASGHPEHGQAQPLAERTAVL
                     QDV"
     gene            complement(1394..>4192)
                     /locus_tag="VLL06_05330"
     CDS             complement(1394..>4192)
                     /locus_tag="VLL06_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS30421.1"
                     /translation="ATLDMLPIGVLLLDRQLRILSVNAEAARLLGHSADFCSSKLLQD
                     VWRQQPGTSDHSIVARIHESLHDRRPIQATQTTLSGPGHESYLVEWSYVLLDSDGDTG
                     GLLTIRDLTRERELQQDYDRLACIAEESPSPIVELDSDANLVYANPAMTRLLQRFGYA
                     TGGFPAVFPGDLSGLVQCCIESGQELRGEEVRLAEASFSWIFCPVPSHRLVRGYAVDM
                     TDVKAAQEELRRSADHLYNSNRQLDHALQKAQEAARVKASFLATVSHELRTPMNGVIG
                     MTGLLMDTEPTEEQQSYIETIRQCGEALLNLINDILDYGKIESGKLEMECIDFNLRST
                     VEDVLGQFAERAQTKGLEITGLVHAAVPTSLRGDPGRLRQILTNFVGNAVKFTEQGEV
                     TVQAFLEKDLVDEVVVRFEVTDSGIGIPQDVQVRLFQPFTQADSSTSRKYGGSGLGLA
                     ISKQLAEQMGGNVGITSQPGRGSTFWSTARFLKQTTSSLAIMPSAELTGRRVLIVDDN
                     ESNRIILHHLVTGWGMVDDQARDAASAIALIEQQAENGISYDVAIVDMLMPGKDGLQF
                     AKEMKVHPVGTLVRLVVLTSLIQRGHAELARQAGFVAYLTKPARHDQLSNCLRTVLGL
                     PGLVVEETVAPVTAPAPPLITRHTLAETRSATRVLVAEDNLINQKLTVRMLEKLGFQS
                     DVVGNGQEALAALAHGSYAIVLMDCQMPLVDGFEATRLIRQREAVTQEAATTACPATH
                     HIPIVALTANAMRGDRERCLAAGMDDYLTKPVRKEDLKGILDRWISAPIQVRVDQSDR
                     AEYSVDMTDTRPLPIIFDMAAMLRNIGGDTALLEELVELFLQRYQTLLEEIRSALVGG
                     DRQAVEQAAHMLKGTASNLCASEVVLAAGQLEALGRLGTLVEGPIIYVQIEKSVLRLV
                     RLFEERHRNHPESRKHAA"
BASE COUNT          885 a         1157 c         1188 g          962 t
ORIGIN      
        1 gattcaactt gcgaatgcgg aagtcagcgg gcaaccggtg tttgccggaa ccgaaaccgg
       61 tgcaccgccg tttgtgttgg gcgcaggcga tgccgtcacc tatcaaggca atagcgaaag
      121 tcagtccatt gcggtcggcc cgaaccaaac cacacagatt ctgctccctg gcgatcaagt
      181 gttcgcggga gccacgacga atatctttac cgatctgagc aacttgctcg ttgcgttgga
      241 gacgaacagc ggagctggga tagaagtggg cattggcaat ctcgaccgcg cggtcgatca
      301 gctgagtgtg gcgcaagggc agattggagc cattgccaat cgtctcgaca cggctcacgg
      361 gtcgttgctg gttgtgtccg cggccattca gcaagcgctc tcggatcaaa cagacacaga
      421 catggccacg gtcctgacgg agcttcagat gcagcaaaca gcgtaccagg ccaccagtca
      481 aacgttctca cggctgtttg attcatcgct tctgaacttt ctccgctgat agtcgttctg
      541 aggtttcgaa cgctggcgcc gatacgccag tggaataact cggcagagat tggactgcac
      601 ggatgctggt cctgacacgg agacgcggtg aaggtgtggc cattggtccg gatatccgga
      661 tcgtggtgtt agggattaag aatggacagg ttcgtttggg aatcgaagcg cctcgaacga
      721 tcgaggtgca ccgcgacgag gtctgtgtca ggattcaaga ggaaaatcaa cttgcggcac
      781 gtacgccgat gatcccgctc gaggtctttc aacgattgct acggacgaaa attgcctgaa
      841 cgcaaggagc tgtcgatatg cacatcacgt caacgcgctt tggggtcttg aatgttccgg
      901 acgaaacgct gctgagtttc ccctcaggct tgctcggctt tcccgagtgg acccgctatg
      961 tggtcctcga tcatgacacc gatgcaccat tcaaatggct gcattgtgcg gaagaggggt
     1021 cccttgcgtt tgtggtcatc gatcccgcgt tgttcaatga gaactatcac gtgacgattt
     1081 ctcccgaggc acgtggtgaa gtagaaggga atgaggctga tgaattggga ctcgccgtta
     1141 tcctgaccat tccatcggat gatccatccg ccgtgaccgc taatcttcgc ggtccgctcc
     1201 ttttgaatcc acgtacgaga ctctgtaagc aactcgtcct ttccgaggac tatcccaccc
     1261 gatatccgat atttccagct tccgggcacc ctgagcatgg gcaggcccaa ccccttgctg
     1321 agcgtactgc cgttcttcaa gacgtgtagc tcagtgaaat tccggcgggg cagcagagtg
     1381 gttctgcagc ctattaggcg gcatgctttc gagattcggg atggttccgg tgtctctcct
     1441 caaacagtcg aacaaggcgt agcaccgatt tttcaatttg aacatagatg attgggcctt
     1501 caaccagcgt accgagccgt ccgagggcct ccaattgccc agcagccaac accacttccg
     1561 acgcacagag attactggct gtccctttca gcatatgagc ggcttgttcc acggcctgtc
     1621 gatctccgcc gacgagggct gagcggattt cctccaacaa ggtttgatag cgttgaagga
     1681 acaattcaac cagttcttct aatagcgcag tatcgcctcc gatgttgcgc aacatcgcag
     1741 ccatatcaaa gatgatcggg agaggcctcg tgtctgtcat atccacgctg tactccgcac
     1801 gatcactttg gtcgacgcgc acctgaatcg gcgcagaaat ccaccgatcg aggattccct
     1861 tgagatcttc tttccgcacg ggtttcgtga gatagtcgtc cattccggcc gcgagacagc
     1921 gttctcgatc tccccgcatg gcattggccg tcaaggcgac gatcggaata tggtgtgttg
     1981 ccggacaggc ggttgtcgca gcctcttggg tcactgcttc gcgttggcga atgagcctgg
     2041 ttgcctcgaa tccatcgacg aggggcatct ggcaatccat caggacgatg gcataggagc
     2101 catgggctaa tgctgccaac gcttcttggc cgtttcccac gacatccgat tggaagccga
     2161 gtttttccag catgcgaacg gtgagtttct gattgatgag attgtcctca gccaccagga
     2221 cgcgtgtggc tgaccgtgtc tctgcgaggg tatgtcgcgt gataagtgga ggtgcaggtg
     2281 cagtgaccgg tgcaacagtc tcttcgacga caagtcctgg caggccgagc acggttcgca
     2341 aacagttcga gagttgatcg tgccgggctg gtttcgtgag ataggcgaca aacccggctt
     2401 ggcgtgcgag ttccgcatgc cctcgctgaa tgagcgaggt gaggacgaca agacggacga
     2461 gagttccaac aggatggacc ttcatttcct tggcgaattg taatccatcc ttacccggca
     2521 tcagcatatc aacgatcgcc acgtcgtatg atatgccgtt ctccgcctgc tgctcgatca
     2581 gtgcgatggc tgaggcggca tcccgggcct ggtcatcgac catgccccat cccgtgacca
     2641 ggtgatggag aatgatcctg ttcgattcat tgtcgtcgac gatcaggaca cggcgtccgg
     2701 tcaattctgc cgacggcata atggccaggg aggaggttgt ttgtttgagg aagcgagcgg
     2761 tggaccagaa tgtgctgccg cgccccggtt gactcgtgat gccgacgttg ccccccatct
     2821 gctcggcgag ttgcttcgaa atcgcgagcc ctagaccgct ccctccatat ttgcgtgatg
     2881 tggagctatc cgcctgagta aacggttgga atagtcgaac ttgtacatcc tgggggatgc
     2941 cgattccgct gtcggtgact tcgaatcgga cgaccacctc atcgaccaga tccttctcga
     3001 ggaaggcttg aaccgtcact tcaccctgtt cggtaaattt gaccgcattg ccgacgaagt
     3061 tcgtgagaat ttgacggagc cgtcccggat ctccgcggag gctggtcggt acggccgcat
     3121 gcaccagacc ggtgatttcc agccccttgg tctgtgcccg ttccgcgaat tgacccaaga
     3181 catcttccac cgtcgagcga aggttgaagt cgatgcattc catttcaagt ttgccggatt
     3241 cgatcttgcc atagtcaaga atatcgttga tcaagttcag taaggcctcg ccgcactgac
     3301 gaatggtttc gatataggat tgttgctcct ccgtaggttc tgtatccatc aagaggcccg
     3361 tcatcccaat gactccattc atcggcgtcc gcaactcgtg gctcacggtc gccaggaacg
     3421 aggccttcac tcgagcggct tcctgtgctt tctggagcgc atgatccagc tgacgattgc
     3481 tgttgtagag atggtccgct gaccgacgca gttcctcctg ggctgctttg acgtcggtca
     3541 tatcgaccgc atatccccga accaatcgat gcgagggaac tgggcagaaa atccatgaaa
     3601 agcttgcctc agctaagcgg acttcctctc cccgtagctc ctgtcctgat tcgatgcagc
     3661 attggacaag ccccgataag tcaccaggaa acacggcggg aaatccgccg gttgcatatc
     3721 caaaccgctg gagcaatcgt gtcattgcag gattggcata tacgagattc gcatcggagt
     3781 ccaactcgac aatgggagag gggctttctt ctgctatgca tgccaagcgg tcgtaatctt
     3841 gctgcaactc tctctcgcgg gtcaggtcac gtatcgtcaa caatccgccg gtgtctccat
     3901 cggaatccag caggacatag ctccactcaa ctagatagga ttcgtgcccc ggaccgctca
     3961 gtgtcgtctg ggttgcttgt atagggcggc gatcgtgaag agattcgtga atgcgggcga
     4021 cgatgctgtg gtccgatgtt cctggttgct gccgccatac gtcctgaagc agcttcgatg
     4081 aacagaaatc agctgagtgc cccaggaggc gagcggcttc ggcatttaca gacaggatgc
     4141 gaagctgacg atcaagcagc agcaccccga taggaagcat atccagcgtc gc
//
LOCUS       DATIPF010000210         1807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1543997, whole genome shotgun sequence.
ACCESSION   DATIPF010000210 DATIPF010000000
VERSION     DATIPF010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543997"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VLL06_05335"
     CDS             complement(<1..605)
                     /locus_tag="VLL06_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30422.1"
                     /translation="MSIGSRLVSIPCFVLVALLSHGAFGIACATDSVLPHVVIRHHEL
                     FVQIVPEQHALIAKDRMTLEVPQAPIRFSLAPTLQIDHVTLVHTSAGSDTSMHDVPYE
                     IEHGSAPESAQQVTIPSQAVPAGLVTLDVHYHGAINDPPRDPRHLRFVTPSETAGHIG
                     LEGVYISSESHWYPDVPESLSTYTLLVAVPTGWTVVTQGKAG"
     gene            complement(616..1398)
                     /locus_tag="VLL06_05340"
     CDS             complement(616..1398)
                     /locus_tag="VLL06_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS30423.1"
                     /translation="MIKILVIDDDRMNCELIQSVFTRHGYQVLSATSGIEGLNLFRQQ
                     APRVTILDLRMPEMDGLTVLKEIRAYDPHAPVIILGGGATEEQENQARGLRVTDFIRK
                     GLSLDVLVECISRVVQCPPKSVPALPVSGSLAAADTGETILVVDDEPLVCDLLVQFLS
                     LRGYRALGVKDGSEALSMVEQTPPDLILLDLFMPGMDGVEVLRQLRQREYAGGVIIIT
                     GSHDEERLDEAWAVGPQEVIGKPIDLEQLLTSIQLVLVCREC"
     gene            complement(1688..1804)
                     /locus_tag="VLL06_05345"
     CDS             complement(1688..1804)
                     /locus_tag="VLL06_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30424.1"
                     /translation="MKLPVRLIGVGESVEDLQDFQSGAFVDALLGTSTPSSA"
BASE COUNT          416 a          508 c          526 g          357 t
ORIGIN      
        1 ccggccttcc cttgggtcac gacggtccac cctgtcggaa cggcaaccag cagcgtatag
       61 gtgctgagtg attccggaac atcgggatac cagtggctct cgctgctgat atagacgcct
      121 tccagcccaa tatgtccggc agtttcgctg ggcgtcacaa aacgaagatg gcgcggatct
      181 cgaggcggat cgttgatggc cccatggtaa tgcacatcca gcgtgaccag tccggcaggt
      241 acggcctgcg agggaattgt gacctgttgc gcggattcgg gcgcggaacc atgctcgatt
      301 tcatacggca catcgtgcat cgaggtatcc gatccggcag atgtgtgcac aagtgtaacg
      361 tggtcgatct ggagcgtcgg ggcgagagag aaacgaatcg gggcctgcgg cacctcaagc
      421 gtcatccgat cctttgcgat caaggcatgt tgctcgggaa cgatctgtac gaatagttcg
      481 tgatgccgaa tgacgacgtg tggcagcacg gagtcggttg cacaggcaat gccgaaagcc
      541 ccgtggctca ggagggcgac gagaacgaaa caggggatag aaacaagcct gcttccgatt
      601 gacatcggaa gagttttagc attcgcgaca gacgagcaca agctggatag acgtcagcaa
      661 ctgctccaag tcgatcggct tcccgatcac ttcttgcggc cccacagccc aggcttcatc
      721 gaggcgctct tcgtcgtggc tcccggtgat gatgatcacg ccgccggcgt attcgcgttg
      781 gcggagttgc cggagcacct cgaccccgtc catccctggc atgaacagat cgagaaggat
      841 cagatcgggg ggcgtttgtt ccaccatcga cagggcctca gagccgtctt tcacaccgag
      901 cgcccgataa ccgcggagac tgaggaactg aacgagcagg tcacaaacca agggctcatc
      961 atcaacgacg agaatcgttt ctcccgtatc agccgcggct aggctgcccg acaccggcaa
     1021 agcaggaacg gatttcggcg gacactgcac cacccggctg atacattcaa ccagcacatc
     1081 aagcgacaag ccctttcgga taaaatcagt cacgcgcaat ccccgtgctt gattttcctg
     1141 ttcctcggtg gctccaccgc cgaggatgat gacgggtgca tgaggatcgt atgcacgaat
     1201 ttctttgagg accgtcagcc catccatttc aggcatgcga agatcaagaa tagtgactcg
     1261 cggagcctgt tggcggaaga ggttcaggcc ttcgatcccg ctcgtcgcgg acagcacttg
     1321 atacccatgt cgggtgaaaa cggactggat cagctcgcag ttcatacggt catcgtcgat
     1381 gaccagaatc ttgatcatga ctcagcctca cggacagaaa tgcatggcac ggtgtgtcga
     1441 gatcatgtgt gaattcagcc taacataggt catgccggcg gagaagaaaa tgacgggatc
     1501 ccaccggagg cttcgtaaga tagccgtgcg agatactggt ggcggggacg ggtacatagc
     1561 cttcacacct cgaacgtggg attgcacttc ccttccctgc attcgtattt cacgattccc
     1621 gagcgatgcg ggctagtcgc aacggggtct cggcgattgc agaaggcgtg tctatgaaga
     1681 atagaggtta agcggaagac ggcgtcgatg tccccagcaa cgcatccaca aaggcgccac
     1741 tctgaaaatc ctgcagatcc tcaaccgact cgccgactcc aatcagtctc acgggtagct
     1801 tcagttc
//
LOCUS       DATIPF010000211         4115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_923997, whole genome shotgun sequence.
ACCESSION   DATIPF010000211 DATIPF010000000
VERSION     DATIPF010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923997"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VLL06_05350"
     CDS             complement(<1..557)
                     /locus_tag="VLL06_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HSS30425.1"
                     /translation="MVWDPKDPWSRKSDPLEDALKQAQSQFKNLMPSFGNLLPSSGFR
                     NIALAGLVIFLAWQCVFIVAPDEEGVVKRFGVPVRTVDPGPHLKIPLIESVMQPKVQK
                     LHRIEVGFRTDRQGRQQMLAQEALMLTGDMNILAVQFIVQYKIKESSNYLFNVAEVGE
                     TIGKAAEASMREVVGKGKIDEALTTG"
     gene            complement(789..1979)
                     /locus_tag="VLL06_05355"
     CDS             complement(789..1979)
                     /locus_tag="VLL06_05355"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSS30426.1"
                     /translation="MPIDEITQKVSDRYARAASKGEQVCGPTSYDFAELKSFIPEEVL
                     KISYGCGTPAGLKTVSTGETVLDIGSGGGIDCFEASRLVGPTGHIIGVDMTDTMLAIA
                     RKNAPLVAASLGYPASNVEFRKGTADAIPVVDGAVDLVISNCVINLAPDKRKVFREMF
                     RVAKPGGRFTISDIVADQPVPQYLVHDTEKWGDCLSGALTLTNYIAGMRGVGFLGIHL
                     IKSSPWQVIDGIHFFSVTLTGYKLPAGTPVSAVRYATLRGPFSRAVDELGTTYLRGIP
                     QPITPDTRCLLNQAPLASHFILSSTPLWLDRSDTRWTAVYPTDAPCQWQGYFALLAGP
                     FIEAADDDHHVYRRGEPVEICSKTLTVLGTDGYAPHFAIINRAGRNASDDAVANPPNQ
                     ESFC"
     gene            complement(2188..2565)
                     /locus_tag="VLL06_05360"
     CDS             complement(2188..2565)
                     /locus_tag="VLL06_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HSS30427.1"
                     /translation="MYSLLADLVLVVHLAFVVFVLCGGLLVLKWRWIARLHLPAVVWG
                     AVVEFTGWICPLTPLENWLRVQGGEAGSQDDFTTRYLLPILYPVDLTRDVQLLLGTVV
                     IVVNAGMYCWFWQMQARGAARNQ"
     gene            complement(2578..3126)
                     /locus_tag="VLL06_05365"
     CDS             complement(2578..3126)
                     /locus_tag="VLL06_05365"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HSS30428.1"
                     /translation="MPGPPTVWTLGHSTRPIDEFIGLLRTHQIRLLVDVRTVPRSRYN
                     PQFNTDTLAQSLRDAGLRYRHLSSLGGLRKPKKDSINDGWRNSSFRGYADYMQTDEFL
                     DALDVLMTESRLQPTAIMCAEAVPWRCHRSLIADALATRDWNIRHIMSETKADLHQLT
                     SFAHFEKGTLTYPKPTDSPSLF"
     gene            complement(3181..3513)
                     /locus_tag="VLL06_05370"
     CDS             complement(3181..3513)
                     /locus_tag="VLL06_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30429.1"
                     /translation="MLRDREITLDLPLFQILKDAGARPHPRITVELFDGANHELDLTS
                     TVSRSNDTAVIRGTFKLPSRGDFTFVASGNLLVGTMQLGDRLYKTEHIANGRLRLIEV
                     DPEKLPPE"
     gene            complement(3696..3851)
                     /locus_tag="VLL06_05375"
                     /pseudo
     CDS             complement(3696..3851)
                     /locus_tag="VLL06_05375"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          929 a         1119 c         1178 g          889 t
ORIGIN      
        1 ccggtcgtca gcgcctcgtc gattttgccc ttgccgacca cttcccgcat tgaagcctcg
       61 gccgctttgc cgatcgtttc acccacctct gcgacgttaa agagataatt cgaagactct
      121 ttgatcttgt attgcacgat gaattggacg gccaagatgt tcatatcgcc ggtgagcatc
      181 agggcttctt gcgccagcat ttgctgacgg ccttgccgat cggtgcggaa gccgacctcg
      241 atacgatgga gtttctggac tttcggctgc atgaccgatt cgatgagagg gattttcaaa
      301 tgagggccag ggtctactgt tcggacaggg acaccaaatc gcttgacgac gccttcttca
      361 tctggggcga cgataaacac gcactgccag gcgagaaaga tgaccagccc tgccaacgcg
      421 atgtttctga aaccgcttga aggcaataga ttcccgaaag agggcatcaa gttcttaaat
      481 tgcgattggg cctgcttaag ggcatcctca agcgggtcgc tctttctact ccaaggatct
      541 ttagggtccc agaccatatg cggcgttcgg ttgtaatgga tattcaagga tctttgtccg
      601 cggtacctta gcagactgcc tgtagcgacg caatcacgat aatcgcgcga gttggtctca
      661 cgtacattct tgatgcagga gatctgtacc gctcaaggac cattattcag gcgactgccc
      721 tgccgcatca cgacccagcg cctgcagtac agccgtaaca ataatggtgg gatcaggggg
      781 ggccttcttc agcagaagct ctcctggttg ggaggattgg cgacagcgtc atcactggcg
      841 ttccggcccg ctcggttgat gatcgcgaaa tgaggtgcgt agccgtcagt ccccaatacg
      901 gtaagcgtct tggaacagat ttccaccggc tcccctcggc gatacacatg gtgatcgtca
      961 tcggcggctt cgatgaacgg tccggcgagc agagcgaaat acccctgcca ttggcagggt
     1021 gcgtctgtcg gatagactgc agtccatcgg gtgtcggatc gatccagcca taggggggtg
     1081 gatgacagaa taaagtgaga agccaaagga gcctggttca agaggcaccg ggtgtccggt
     1141 gtgattggct gagggatgcc tcgtagatac gttgtaccga gttcatcgac tgctcggctg
     1201 aacggaccgc ggagcgtggc ataacgaacg gctgatactg gcgtgccggc tggaagttta
     1261 tatcccgtca acgtgacgga gaagaagtga atgccgtcga ttacctgcca gggcgaggac
     1321 ttgatcaaat gaatacccag aaaaccgact cccctcatcc ccgcaatata gttggtgagc
     1381 gtcagcgctc cggaaaggca gtcgccccac ttttccgtgt catgcaccaa gtactgcgga
     1441 acgggctgat cggctacgat atcggagatc gtaaatcgtc cacccggttt ggcgacgcga
     1501 aacatttccc taaagacttt ccgtttatca ggcgccaggt tgatcacgca attggagatg
     1561 accagatcaa ctgcgccgtc aaccaccggt atcgcgtccg ccgtgccttt ccgaaactcg
     1621 acgttggaag caggatagcc aagactggcg gcgacgagag gggcattctt ccgcgcaatc
     1681 gccagcatcg tgtcggtcat gtcgactccg ataatgtgac cagtgggacc gacgagacgg
     1741 gaggcttcga aacaatcgat gcctccaccg gaaccgatat ccagcaccgt ttcccctgtg
     1801 gagaccgtct ttaacccggc tggtgtgccg caaccatacg aaatcttgag tacttcctcc
     1861 ggaataaatg atttgagctc ggcgaagtca tagcttgtgg ggccacagac ctgctcaccc
     1921 ttcgatgcag cccgagcata gcgatcgctg accttttggg taatctcatc tattggcatt
     1981 gcggcctcac ggtcgaatga aaatatgcac gactttccat ctatcaactt tggtaccgat
     2041 tcgtcaactc catgttcacc gcgattcccc gttgcgaatc aacgggggag ttgagtttcc
     2101 tgtggcagca ttccttggta tggcctacac ataaatcggt gtcatcctag ataggagatc
     2161 gataatactt gcctgtgcga ctgaacccta ttgattccga gcagcaccgc gcgcctgcat
     2221 ttgccagaac cagcagtaca tcccggcatt gaccacgatc accactgtgc ccaacagcaa
     2281 ttgaacgtca cgtgtcaggt ccacagggta gagaatcggc aacagatagc gtgtcgtgaa
     2341 atcgtcttga gagccggcct caccaccctg cacgcgcaac caattctcca gcggtgtgag
     2401 agggcagatc cacccggtga attcgacgac cgcgccccac acgactgccg gtaggtgcag
     2461 ccgcgcaatc caccgccact tcagcacaag cagaccaccg cacaggacga agaccacgaa
     2521 cgccaggtga acgaccagga ccagatcggc caggagggag tacatcgtgg caccctgcta
     2581 gaacaacgaa ggggaatcgg tcggtttcgg ataggtcagc gttcccttct caaagtgagc
     2641 aaatgacgtc agttggtgga gatcagcctt cgtctcagac atgatatgcc ggatgttcca
     2701 gtcacgggtt gccagcgcat cggcaatcaa cgaccgatgg cagcgccacg gcaccgcttc
     2761 tgcgcacatg atggctgtcg gctgcaaccg gctctcggtc atcagcacgt ctaaggcatc
     2821 caagaattca tccgtctgca tatagtccgc gtatccccga aaactagagt tgcgccatcc
     2881 gtcgtttatg gaatccttct tcggctttcg taagcctccc aacgacgaga ggtggcgata
     2941 ccggagcccg gcatcgcgca atgactgggc cagcgtatcc gtattgaact gcggattgta
     3001 gcgggatcga ggaacggtgc ggacatcgac gagaagacgg atctgatggg tgcgcaacag
     3061 gccgatgaat tcatcgatcg gcctcgtcga gtggccgaga gtccagactg tcggtggccc
     3121 aggcatagat cagtgtggca cggcggacag ggaatcgcca gcggttaaaa aggaagagga
     3181 tcactccggc ggcaattttt cgggatcgac ttcgatgagc cgcagtcgac cgttggcgat
     3241 gtgttcggtc ttgtagaggc ggtcacccaa ctgcatagtc ccaaccagga gattgccgct
     3301 ggcgacaaac gtaaagtcgc cccgtgaagg caacttgaag gtaccacgga tgacggcggt
     3361 gtcgttgctg cgggagaccg tcgacgtgag atccaattcg tggttggctc catcaaagag
     3421 ctcaacggtt attctgggat ggggtctggc tccggcatcc ttcaatatct gaaagagtgg
     3481 gaggtcgagc gtgatctctc gatcgcgaag cacccagggt ctccgcggca tcggtgattg
     3541 atcggcggtg ggagtagtca ggactgtctg ccataaactt tttgctgccg ggcgctcttc
     3601 ggcccaggcg ggaaaagaac agacacaact gaatagtccg acaagaagca ggcgtctgaa
     3661 tggaacacag ttgagcctgt gccacgcgaa tgttatcaga ggctgctggc ctgctgagcc
     3721 catagcgtca gttctcccgc atcttcaagg atatccaccg ggacttcgta ataggagtgg
     3781 agtatttgct tggcattggg tctgtacgct ttcatcttgc gctcgcggta tcgtcgtgcc
     3841 gtggtttcat tggtcttaag tagagccggc ccttgtggac gatgccgaag aacaccctac
     3901 cacaatatag cccatatccg ccgaacatgg cgcggtgagt cagacttggt agatcgctga
     3961 gttgatccag cacgaagttc ttgaatccgt catgcttgag tgccacggaa ttgtcacaga
     4021 ggtttacgtg ccgcgaccat tcggacggcg atgggaaagg tgattctgtt gccacgctgg
     4081 taggggattg cagcctgagt gatgacgcgt cgcac
//
LOCUS       DATIPF010000212         3708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1013372, whole genome shotgun sequence.
ACCESSION   DATIPF010000212 DATIPF010000000
VERSION     DATIPF010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013372"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            697..1149
                     /locus_tag="VLL06_05380"
     CDS             697..1149
                     /locus_tag="VLL06_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSS30430.1"
                     /translation="MLVHEVMSTGLVTAKKTDTIRSVVKKMINRHCATIPVVEGDNLL
                     IGLVTLRDVLLPLYPNYGDYIHDNVHSRNFVEMEEGYPELLGKKVEEVMSGNPLTVTP
                     YDPILEVASYMGLKNFRRIPVVDKGVLVGMVSIGDINRGLFFEKGKER"
     gene            complement(1213..1749)
                     /locus_tag="VLL06_05385"
     CDS             complement(1213..1749)
                     /locus_tag="VLL06_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSS30431.1"
                     /translation="MMSQLPPTTIPRENTHLSRILIAVDGSEHSARALRYVGTLLRDT
                     RDVQITLFHVLKPMPRALLEHGGSEDPAEEIRLAKKLQEDQDSWVRTESVFESPILVT
                     ALELFGKTGFPLDRVALKFGHEDDIAHTILDEARTGGYGTIVMSRHASNAIKRFFGGS
                     ITDQLLRDASGFTLWIVE"
     gene            complement(1746..2234)
                     /locus_tag="VLL06_05390"
     CDS             complement(1746..2234)
                     /locus_tag="VLL06_05390"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HSS30432.1"
                     /translation="MSVSPSLNAYLDHERVHYDVLPHPEAFRAAEIAHTLHTPEKDMA
                     KVVIVKVNERFVMTVLPANWSVDLHRLRDVFLTHHVQLATEHEITGLFPDCELGAMPP
                     FGNLYGLPVYVDQSLTEDKEIVFQAGTHSDSIRMRYMDFAALVFPVVAEFHRSPSESG
                     NS"
     gene            2233..2364
                     /locus_tag="VLL06_05395"
     CDS             2233..2364
                     /locus_tag="VLL06_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30433.1"
                     /translation="MVTPPLVGDLRIEVISSIVGGDAGLLNGDVTVAKTCFMIKAPQ"
     gene            2740..>3708
                     /locus_tag="VLL06_05400"
     CDS             2740..>3708
                     /locus_tag="VLL06_05400"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HSS30434.1"
                     /translation="MLLQRDIQTVAKTREQRFLGKVPDTTARCLGGKYAESLREGPWL
                     DWPNYWAAGDATSLAPARLLSNAKVIGPNAHGINAALYELELQRIELIKFNLFDNNKT
                     YEAYITGRDGEAGPVLRTWPEMRLPRSHPEFTAVGGHQTQVCRGEFIRFRTVTGICND
                     IRNPLMGSTHQLFARNVEFDTTFPDLGLNEMTKNRHGDRLGLLKPDPQIISRKLFTRA
                     QADPKKCDKGYGLPGGVKEAECDYEKAPFFNVLAAFWIQFMTHDWFAHLEEGHNKPEW
                     IAVGCATQLVENVEKPVTGNEADKLGCRPGDKMDLAYIAEDTKPKTF"
BASE COUNT          877 a          955 c         1039 g          837 t
ORIGIN      
        1 aggggattgg tcgacttcgg cgcgcaaagc gctgttcacg ggcgacccta tacttggtat
       61 agggtcgccc gtgctgtttc aaggtcgagg gtctgagaaa gtgaggaaag tactctcgtg
      121 cagtattcct gtaaagtccg cgtatgtttc agaacttcac cacaagccat gacgattgca
      181 tgtcatcata ctctatgctc tatggccaat tcggccaccg cgtcagacaa cgaagagaag
      241 ttcttccatt gacataaaga tgaaagcggg agtaagaaac aaagcggctg tacactcatc
      301 ttcagagagc aggtctctaa tggaagacct cggtccaccg gatccgattg ttccgcagaa
      361 ttgaagatgg cgcgcaaggc cggttcatca gccaatctcg gacggttacg tctttcccat
      421 gtatgggaac tacccgacga ggctcctctg cgtgatggag gcagaggttc tccgccgatg
      481 aatctcaaga ccttcaatcg cgtagacggt ttctcctgtc ctgtagcgat agaaataccg
      541 gtcatcggca ccctaagcgt ctgccattgg tacgataggg agggcttcat tcctaccttc
      601 cttgacagtc acccatggca gttctcgtgc tgatatcacc aggtggcttt gaacggagag
      661 gcatcttggt gatcaatcac cagaagggag gtggccatgc ttgtccatga agtcatgtcg
      721 accggtctcg tgacggccaa gaagacggat acgattcggt ctgtcgtgaa gaaaatgatc
      781 aaccgccatt gtgcaaccat tcctgttgtt gaaggagaca atctgcttat tggattggtc
      841 accttacgtg atgttttgtt gccgctgtat cccaactatg gcgactacat tcatgacaat
      901 gtacacagcc ggaattttgt cgagatggaa gaggggtacc ctgaacttct cggaaaaaaa
      961 gtagaagagg tcatgagcgg aaatccctta acagtgacac cctacgaccc cattctggaa
     1021 gtcgcctcgt acatgggcct caagaacttt cggcgaatcc ctgtcgtcga caagggtgtg
     1081 cttgtcggaa tggtcagtat cggcgacatc aaccgtggat tattcttcga aaaagggaag
     1141 gagcgctgac tcacaccttc ctcaaggaac gctcatctca tccagctcag tggtcatgac
     1201 gtgcccattg gatcattcta cgatccacag cgtaaagccc gacgcgtcac gcagcagctg
     1261 atccgtaata cttccaccaa aaaaccgttt tatcgcattc gaggcgtgac ggctcatcac
     1321 gatggtccca taacctccgg tgcgggcttc gtcgaggatc gtatgcgcga tatcgtcttc
     1381 gtggccgaac ttcagcgcga cgcggtccag aggaaatccg gtcttgccaa atagctcaag
     1441 cgcagtcacc aagatagggg attcaaacac gctttctgtg cggacccagc tatcctgatc
     1501 ctcctgcaat ttcttggcta gacgaatttc ttccgcggga tcctcggatc ccccatgttc
     1561 taggagcgcg cgcggcatcg gcttcagcac gtgaaacagc gtaatctgca cgtcgcgagt
     1621 atcacgtagg agagttccca catagcgcaa cgcccgggcg gagtgctcag agccatcgac
     1681 tgcgatcaaa attcgcgaga gatgagtatt ctcacgcgga attgtcgtcg ggggaagttg
     1741 tgacatcatg agttcccaga ctctgagggg gaacggtgga attccgctac caccggaaac
     1801 acgagcgcgg caaagtccat atagcgcatc cggatcgagt ccgaatgggt tccggcttga
     1861 aagacgatct ccttgtcctc tgtgagcgat tgatcgacat acacaggcag cccatagagg
     1921 ttgccgaagg gtggcatcgc gcccaattcg cagtcaggaa atagaccggt gatctcatgt
     1981 tcggtggcaa gttgcacatg gtgggtcaga aagacgtctc tgaggcgatg gagatccacg
     2041 ctccagtttg ccggcaggac cgtcatgacg aaccgctcat tcactttcac aatcacgacc
     2101 ttggccatgt ccttctctgg ggtgtggagc gtatgggcga tctctgcggc gcgaaacgct
     2161 tccggatgtg gcaacacgtc atagtgaacc cgttcgtgat cgagatacgc attgagtgat
     2221 ggtgagacag acatggtgac gcctcctctg gtgggcgacc tcagaatcga ggtcatttcc
     2281 tcaatagtcg ggggagatgc cggactgctc aatggcgatg tgactgtggc gaagacgtgt
     2341 ttcatgatca aggctccgca gtgatcatga ggacttaatt ttcctagtat acgccggttc
     2401 aatgtccacg tcttggggaa agagtcacat aaattcctct gcatcgctat actccagtca
     2461 acgcggtcaa aagaatcatc accgagattt cccccaaagc tgcgttccac gaaatacatg
     2521 tattgcctgt tagcgaatgt tgattctttt gtggctgaga cgtagcctgt ggccagttgt
     2581 gtggcgtgca atgattcctg tcgttgagga gagctgcgta tgaaaccaaa gtgtatatgg
     2641 tcacggctta ttatcgtcgc tggactctgt gttcttgggg ggtgcgccag gtatagccct
     2701 ttcgatatct tcgtggactc cacgaaatat tgtgcagaga tgctcctaca acgagatatt
     2761 cagacagtcg cgaagacccg agagcaacgc ttcttgggga aggttcccga caccaccgcg
     2821 cggtgtctcg gagggaagta tgccgagagt ctcagagaag ggccgtggct ggattggccg
     2881 aattattggg ctgccggcga tgccacgtct ctagcgcctg cgcggcttct gtccaacgcc
     2941 aaggtgatcg gccccaacgc acatgggatc aacgccgcgc tctatgagct ggaactgcag
     3001 cgtatcgaac tcatcaagtt caatctcttt gacaataaca agacctacga ggcctacatc
     3061 acaggccgtg acggcgaggc cggaccggta ttgagaacct ggccggagat gcgattgccg
     3121 cggagtcatc ccgagttcac ggctgtcggg ggtcatcaga cacaggtctg tcggggagag
     3181 ttcattcgtt tccgcacagt cacgggcatc tgtaacgaca tccgcaatcc gctgatggga
     3241 tcgacgcacc agctgtttgc acgaaacgtg gagttcgaca ccacatttcc cgatctaggc
     3301 ttgaacgaga tgaccaagaa tcgccatgga gatcgattgg gcctattgaa gccggatccg
     3361 cagatcatta gccggaagct gttcacccga gcgcaggcgg acccgaaaaa gtgcgacaag
     3421 gggtacggtc tgcctggggg tgtaaaggaa gccgagtgtg actatgagaa ggcgccattt
     3481 ttcaatgtgc tggcggcctt ttggatccag ttcatgaccc acgactggtt cgctcacctg
     3541 gaagagggac acaataagcc tgagtggatc gcggtcggct gcgcgacgca actggtcgag
     3601 aatgtcgaaa agccggtgac gggtaacgag gctgacaagt tgggctgccg tcctggcgac
     3661 aagatggatt tagcctatat cgccgaagac acgaaaccga agacattc
//
LOCUS       DATIPF010000213         5441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_838105, whole genome shotgun sequence.
ACCESSION   DATIPF010000213 DATIPF010000000
VERSION     DATIPF010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838105"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VLL06_05405"
     CDS             complement(<1..361)
                     /locus_tag="VLL06_05405"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HSS30435.1"
                     /translation="MTKLVSLAIMLCLVCFAGLAQATNYERSKIQLPEPRGYVSDHAQ
                     AIDGDWKERIRSVCQDLERKTGIEMVVVTVPSIKPFASANEYASAIYEKWGIGSTQQE
                     HGVLVLVAVEERQAAITL"
     gene            complement(358..1116)
                     /locus_tag="VLL06_05410"
     CDS             complement(358..1116)
                     /locus_tag="VLL06_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30436.1"
                     /translation="MTERLIEGLPGEIQKLLQTYVKEVKGQFGDQLEGMLLYGSAVRG
                     EFLLGRSNLNIFLLVSSYDSAVLKQYSALHRRWSKEQIVVPLFLTEEELHLSAAVFPL
                     EFLEIQEQHRVLGGRDPFIGFHVKTDRLREAVVQGLMSNLLRLRQRYVEGGGSGEATT
                     LLLPLSITSTVPLLRGVQRLLGRPVLSHSDAVIKDVAEQLNLDLQGLLDALLLKRGQI
                     SPGPHEVPRLFDRYLQAATLVSRTVEQLSQSEQR"
     gene            complement(1128..2432)
                     /locus_tag="VLL06_05415"
     CDS             complement(1128..2432)
                     /locus_tag="VLL06_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HSS30437.1"
                     /translation="MGSALNGMADSIEETDVVVVGAGGGGAVLALALAKQGISTVVLD
                     QATGPPKGLRGEILQPNGQQVLDRLGVLPSLPADATRSVRHFHFCRAGGKRLCSIDYG
                     DLPAPYNRAIVTLPNVAHHAIVAAVQQEKSVTLRYGTTFTGLLRESDRVVGLSTKGPA
                     GTNMIRAKIVVGADGAFSKVREALKIPADLYLYPDGYLIAILESEEPVSESFYYIGAK
                     TILGLFPATGNKVYIFYMIPKDSMETVKQRGLPALHETWSRIAPQFTGLFRHLNDWSQ
                     TAYMPTGRVRTPTWVADGAVLIGDAAHAMNPHASQGRMQAMVDAMALSDLLSGCLADK
                     DCSAARLHAFERARRPQVTMLQQLADQQVFYWNTGNPVVAFLRDRVFQTLDRNARLRY
                     QVLSTTAGLRTIPPFSGIDRMIAAGLFPDVCTDERSQHPMSS"
     gene            complement(2417..2956)
                     /locus_tag="VLL06_05420"
     CDS             complement(2417..2956)
                     /locus_tag="VLL06_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HSS30438.1"
                     /translation="MHNWIIRWLCLVVLMLAVPVGSGWSATVEVYYGPDDAPLDRLTA
                     LYRHATRYLYVAVYGLTSPRAVEAMVAAKKRGVDVRLITDRQRTEDVKQRTALHTLHL
                     AGIPILVNEHDGLMHVKQVVIDDEVNTTGSMNHTTSGNRYNDERLDVITDHAMSVKAR
                     EKFLAMWNDHSRYRVWDQP"
     gene            complement(2992..3369)
                     /locus_tag="VLL06_05425"
     CDS             complement(2992..3369)
                     /locus_tag="VLL06_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30439.1"
                     /translation="MWNRNFMFRSMEAIPLKESENELFHDTDPAMDSTGLQLEKFLSV
                     WIQGDGDGDTPSAFTNMYVRTATLDFQKRVGFLQPLQGRSHQIKQLLTSGQKQFLQQW
                     LAAEAPKAWEATDDHFKMLFEVE"
     gene            3519..3719
                     /gene="thiS"
                     /locus_tag="VLL06_05430"
     CDS             3519..3719
                     /gene="thiS"
                     /locus_tag="VLL06_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HSS30440.1"
                     /translation="MQVKINGKSEEIVGGTVLDLLQTKKIEPQMVAVEVNDTMIDRDH
                     LTTTRLNEGDRVEFLFYMGGGR"
     gene            3719..4645
                     /gene="cysK"
                     /locus_tag="VLL06_05435"
     CDS             3719..4645
                     /gene="cysK"
                     /locus_tag="VLL06_05435"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HSS30441.1"
                     /translation="MMTMHKDITELIGRTPLVRLNRLSPVGGATIYGKVEFFNPGGSV
                     KDRICLNMINEAERLGKLKPGSTIVEPTSGNTGIGLALVAAVRGYKLILVMPESMSLE
                     RASLLSSYGAQLVLTPAWEGMKGSIREAESLIAQNPSYFMPDQFSNPANPAMHRKTTA
                     IEILEALDGKIDAFVAAVGTGGTITGCGELFKERNPDVKVIAVEPAGSPVLSGGEAGP
                     HKIQGIGAGFIPKVLNQAILDRVMTVTDDEAYQTAKQLSRKEGLLVGISAGANVFAAQ
                     KVAQELGPGKNVVTILCDTGERYISIEKYFNI"
     gene            4868..>5441
                     /locus_tag="VLL06_05440"
     CDS             4868..>5441
                     /locus_tag="VLL06_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HSS30442.1"
                     /translation="MEFTEDQINRYSRHILLPEVGGKGQKKIAKAKVLLVGAGGLGSP
                     AALYLAAAGVGTIGLIDSDVVDMTNLHRQILHYTSDVGRPKVLSGKEKIQALNPDVKV
                     SMYEERLTAGNALKIISEHDVVIDGVDNFTAKFLINDACFFAGKPLIHGGILRFDGRV
                     TTIVPKQSACYRCIFKAPPPAGLVASCQEAG"
BASE COUNT         1228 a         1570 c         1508 g         1135 t
ORIGIN      
        1 cgagagtaat cgcggcttgc cgttcctcca ccgcgaccag caccaacacc ccatgctctt
       61 gttgtgtcga tccgataccc catttttcgt aaatcgcaga tgcatactca ttcgccgacg
      121 caaacggctt gatgctcggc actgtgacca ccaccatctc gatgccggtc ttccgctcaa
      181 gatcctggca gacagaccgg atgcgctctt tccaatcccc atcgatcgct tgcgcatggt
      241 cgcttacata acctcgcggc tcaggcaact ggatcttgga ccgttcgtaa ttcgtcgcct
      301 gggccaggcc cgcaaagcag acgaggcaga gcattatcgc gagactgacg agctttgtca
      361 tcgctgctcc gactgtgaca gttgctcgac tgtgcgagaa accagcgtcg cggcttggag
      421 atagcggtca aagaggcgag gcacctcgtg ggggcctgga gaaatctgtc cgcgcttgag
      481 caacaaggcg tccagcaaac cctgcagatc caaattcaat tgctcggcga cgtccttgat
      541 caccgcgtcg gagtgggaca gcacgggccg cccgagaaga cgttgcaccc cgcgcaataa
      601 cggaacggtt gacgtaatcg aaagcggcag taatagggtt gtagcttccc cgctcccccc
      661 cccttcgaca taccgttggc gcaggcgcaa cagattgctc atgaggccct ggaccacggc
      721 ctctctcaac cgatcggtct tcacatgaaa tccgatgaag ggatcgcgtc ctcctaagac
      781 gcgatgctgt tcctgaattt ccagaaactc aagcggaaat accgctgccg acaggtgcag
      841 ttcctcttct gtcagaaaga ggggaacgac aatctgttct ttgctccatc ggcgatgcag
      901 cgcgctgtac tgtttgagaa cggcactatc gtacgacgac accaggagga agatattcag
      961 gttggaccgc cccaggagaa attccccccg gacggcactc ccatagagca gcatgccttc
     1021 cagttggtcc ccgaactggc ctttgacctc cttcacatag gtctgcagca gcttctgtat
     1081 ctcgcccggc aatccttcga tcagacgttc agtcatgccc gtggtcccta tgaagacatc
     1141 ggatgttgcg atctctcatc cgtgcagaca tcgggaaaca atcccgcagc aatcatgcga
     1201 tcgatcccac taaacggcgg tatcgttcgc aaccctgccg tggtcgacag gacctgatag
     1261 cggagccgtg cgttccgatc cagcgtctga aatacacgat cgcgcaggaa ggccaccacc
     1321 ggattccccg tattccaata gaacacttgt tgatcggcca attgttgcag catcgtcact
     1381 tgcggtcgcc tggctcgttc aaatgcgtgc agcctggcgg ctgaacagtc cttatcggca
     1441 agacagcctg ataacagatc ggacagtgcc atggcatcga ccattgcctg catgcgcccc
     1501 tgcgacgcat ggggattcat tgcatgggcg gcatcgccaa tcaggacggc gccatctgca
     1561 acccatgtcg gcgtgcgcac ccgcccagtc ggcatatagg cagtctggct ccaatcattc
     1621 agatgccgaa acagtccggt aaactgcggc gcgatcctgc tccacgtctc gtgaagcgca
     1681 gggagacccc gttgcttcac ggtctccatc gaatccttgg gaatcatgta gaaaatatag
     1741 accttgtttc ccgtcgccgg aaacagtccc aagatggttt tggctccgat atagtagaac
     1801 gattccgata ccggctcctc cgattcgagt atggcaatca gatacccgtc aggataaaga
     1861 tacaagtcgg cggggatctt cagggcctcg cgcactttcg aaaatgcgcc gtcggcgccg
     1921 acgactatct tcgcacggat catgttggtg ccggccggcc ctttcgtgga caaccccacg
     1981 actcggtcgc tctcgcgcaa gagtccagtg aaggtggtcc cgtagcggag cgtcacgctc
     2041 ttctcttgct gcaccgccgc gacaatcgca tggtgggcca cattcgggag tgtcacgatg
     2101 gcgcggttgt acggcgccgg cagatcgcca tagtcgatac tacagagacg cttccctccg
     2161 gcccggcaaa aatgaaagtg ccgcacggac ctcgtggcat cggccggcaa tgatggcaac
     2221 acgcccaagc ggtcgagcac ttgttgcccg ttcggctgga ggatttctcc tcgcaagccc
     2281 ttcggtgggc cggtcgcttg atccaacacc accgtgctga tcccttgctt cgccagcgcc
     2341 agggctagga cggctcctcc accccccgcc cccaccacca cgacatcggt ctcttcaatc
     2401 gagtctgcca tgccgttcag ggctgatccc acacccgata tcgactgtga tcattccaca
     2461 tcgcaaggaa cttctctcgg gccttgacgc tcatcgcgtg atcggtaatg acatcgagcc
     2521 gctcatcgtt atagcgattg ccgctcgtcg tatggttcat agagccggta gtattcactt
     2581 catcgtcgat caccacctgt tttacatgca tcaacccatc atgctcgttc acgagaatgg
     2641 gaatgccggc caaatgcaac gtatggagcg ccgtgcgctg tttgacgtcc tcggtacgct
     2701 gccgatcagt gatcaaacgc acatcgactc cccgcttctt cgctgcgacc atcgcctcca
     2761 ccgcacgggg agaggtcaac ccatagaccg ccacatatag atagcgtgtc gcatgccgat
     2821 agagcgccgt cagacgatcc agtggtgcgt cgtccggccc gtaatagacc tccactgtcg
     2881 cgctccagcc agacccgacc ggaaccgcaa gcatcagcac gacaagacac aaccacctta
     2941 taatccagtt gtgcatgcct attctcatcg acggtagaga acggaccgga gtcattcgac
     3001 ttcaaacagc atcttgaaat gatcgtccgt ggcctcccag gccttgggtg cctcagcggc
     3061 gagccactgc tgcaaaaact gcttttggcc ggacgtgagc agctgtttga tctggtgcga
     3121 gcggccttga aggggttgca gaaacccgac ccgcttctga aaatccaatg tggccgtgcg
     3181 gacatacata ttcgtaaagg ccgatggtgt gtcgccatcc ccatcgcctt gaatccatac
     3241 cgataaaaac ttctctaact ggagtccggt gctatccatg gcgggatcgg tatcgtgaaa
     3301 caactcgttt tccgattcct tgagcggaat ggcctccata gacctgaaca tgaagttgcg
     3361 gttccacatg cgatcgcgtc tccaaaagac ggcatagtgg cggcggatca cggacaaagt
     3421 caagccgcgt gccagccgct ccgttacctt gacaacgaag aagtccgttt gataacgtgt
     3481 tgaaattgtt taatctatgg gccatgttag gagccttcat gcaggtcaaa atcaatggga
     3541 agtcagagga gattgtaggg ggaacagtcc tcgatcttct tcagaccaaa aagattgaac
     3601 cgcagatggt tgcggtcgag gtaaacgata ccatgatcga ccgggatcat cttaccacca
     3661 cccgcctgaa cgaaggcgat cgggtcgagt tcttgtttta tatgggcggt ggccggtgat
     3721 gatgaccatg cataaagaca tcaccgaact catcgggcgg actcccctcg tacgtctgaa
     3781 ccggctatcc cctgtcggcg gggcgacgat ctatggaaag gtcgagttct ttaatcccgg
     3841 tgggagtgtg aaggaccgaa tctgcctcaa tatgatcaat gaggcggaac gactgggaaa
     3901 gttaaagcca ggcagtacga tcgtcgaacc gacgagtgga aataccggga tcggactggc
     3961 gttagtcgcc gcggttcgtg gctataagct gatcctcgtg atgcctgaga gcatgagcct
     4021 ggagcgagca agcctcttgt catcctatgg cgcacaactc gtcttgacgc cggcctggga
     4081 agggatgaag ggctcgatcc gcgaggcgga aagtctgatt gcgcaaaatc cgtcgtactt
     4141 tatgccggac cagttttcca atccggccaa tccggccatg catagaaaga ccacagcgat
     4201 cgagattctc gaggcgctcg acggaaagat tgatgcgttt gtggcggccg tgggcaccgg
     4261 cgggaccatt accggatgtg gggaattgtt caaagagcga aatcccgacg tgaaagtgat
     4321 tgcagtggaa ccggccggat cgccggtctt atccggtggg gaagcagggc ctcataagat
     4381 tcagggaatc ggtgctgggt tcatcccaaa agtgttgaac caggccattc tggatcgtgt
     4441 gatgaccgtg accgacgacg aggcctatca aacggccaag cagctctcga ggaaggaagg
     4501 tcttttggtg ggaatttctg ccggggccaa cgtattcgca gcgcagaaag tagcccaaga
     4561 gcttggacca ggcaaaaatg tggtcacgat tctctgcgat acgggcgaac ggtacatcag
     4621 catcgaaaag tatttcaata tttgagatct ctttcataaa gtgaaccagg agggatgctc
     4681 aaaatggcca gccaacgagg ccgcaggcga gaagaaaccg gaggcgtacc cgctggggcg
     4741 cacggtgcga cgaataagga gcaccaagtc tgtgcgcgcc gccgagtggt gaggcggccg
     4801 ggtccccgtt gaggatttct tcgaaccgag aacgaagttg ggggtcattt tcagcatccc
     4861 gtgaaacatg gaatttactg aagatcagat aaacaggtac agccggcata ttcttttgcc
     4921 tgaggttggt ggcaaggggc aaaagaagat tgccaaggct aaggttcttc tcgtcggcgc
     4981 tggaggcctt ggttcgccgg cagcactcta tctggccgca gccggtgtcg gcacaatcgg
     5041 cctcatcgac agcgatgtgg tggacatgac caatcttcac cgccagatac tgcactatac
     5101 gtcggacgta ggtcgcccca aagtcctttc gggcaaagaa aagattcagg cgttgaaccc
     5161 cgacgtcaag gtttcaatgt acgaagagcg gttgacggcc gggaatgcgc tgaaaatcat
     5221 cagcgagcat gacgtcgtca tcgacggggt cgacaacttc acggccaagt tcttgatcaa
     5281 cgatgcctgc ttctttgccg gaaaaccgtt gatccatggc ggcattctcc gattcgacgg
     5341 ccgcgtgacg accattgtgc cgaagcagtc cgcctgttat cgttgcatct tcaaggctcc
     5401 gccaccggcc ggactggtcg cctcctgcca ggaggcaggg g
//
LOCUS       DATIPF010000214         3387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1273907, whole genome shotgun sequence.
ACCESSION   DATIPF010000214 DATIPF010000000
VERSION     DATIPF010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273907"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..287)
                     /locus_tag="VLL06_05445"
     CDS             complement(15..287)
                     /locus_tag="VLL06_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30443.1"
                     /translation="MYQEEKTFTFRFSLEASFPDEYEGDEENQAWVREWEARIKPDLL
                     KVLFESLRQHKAWKAHVRNRGISPADEIEIVLARDFSKPQGFSLLS"
     gene            729..953
                     /locus_tag="VLL06_05450"
     CDS             729..953
                     /locus_tag="VLL06_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30444.1"
                     /translation="MKPTDAGLRAVTASWMGIYQKVIESGGFTTQALRRIDPHPRVAL
                     LIESGMLTDDQQAVADLRTSFERAIAAATE"
     gene            964..1629
                     /locus_tag="VLL06_05455"
     CDS             964..1629
                     /locus_tag="VLL06_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30445.1"
                     /translation="MWVLWGIAVLLLTVGSVGEGRAEEALGTRPLLAIAPKDLHTILP
                     QDSHILIEPRSLEQFLDSLDKTPPDWDLVYGHGHDDPGHDERLFTLNRERDAKRAGNS
                     ALTQRITFVWFGELSRYDAEAGGFRIALGPRLTPTRWGVVRFKCEDLPSNLVVKVSSS
                     LRHELEQRVARGESVDLTVALTGQLIPDESVVYDFSHDQEGVGVIMPVVRVERLDYLL
                     TNK"
     gene            1939..>3387
                     /locus_tag="VLL06_05460"
     CDS             1939..>3387
                     /locus_tag="VLL06_05460"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HSS30446.1"
                     /translation="MKRTRRRQIESISITKFFGTAVVLAFGFHGLVSGMVGVTSLYAS
                     EAVTESGRSLMKSDSHRNNEGLATIAGTKPSLKLGMKSDSSNVGKKPRTLIRKPSSGG
                     SQTESPQQADQSLPSSTPLGGGQAQSATKDSESAIARSGAGMSSVAPPALSGSSVVTT
                     SPGISAAGAIAGAATASPGSSMGVAGSDGSSSGGGRSAQNLMRQMPELQQLLATHSGP
                     PPEMIAAPAPPLGPPQAGLPPPPANWPAYHVLNRLSYGGTPNQLNTISHLTVQEARDW
                     AIRYMKEQLELDPDKPWPTNLHNTNPLPAPIVLDDSALSQRLAADRADWRSEDGLPDI
                     LSPLLSELQDYDLIRKAYSRRQLKEKMVYFWDNHFNTNFRTHSKGQYELAENESFRAN
                     AFGKFIDLLMVSAKSSAMMIYLNTDVNRKENPNENYARELQELHTLGVDVNGNPNGYM
                     QADIVEAAKTFTGWTTLDMARPGFRFISGWHSP"
BASE COUNT          748 a          927 c          959 g          753 t
ORIGIN      
        1 aaggggaggg gttgtcatga caacaggctg aatccttgtg gtttggagaa gtcccgtgcg
       61 agcacgattt caatttcatc ggcaggggat atcccccggt tgcgcacatg tgccttccat
      121 gccttgtgct gacgcaacga ttcaaacagc accttgagca gatcgggttt gattcgcgct
      181 tcccactcgc ggacccaggc ctggttctcc tcatcgccct catactcatc ggggaaagag
      241 gcctccagac tgaatcgaaa ggtaaaggtt ttttcttctt gatacataca gctcctcagg
      301 agcctatctg acattgaccg aagcaatgtc ggataggctc ctagcagatt gcgaagtggc
      361 cggtccgatc ttataatacc cccaaattaa ggagtatcac ccatgcctat cttcgaatat
      421 gtctgcgggg aatgcaatca tcggttcgag ctcctggtgt acggctcaat gcaagccgcc
      481 tgtccaaagt gccgcaccac gaagttggag aaacaaatct cttcgtttgg tgtcggtggc
      541 acagatggct gggtgttgcc gggtaatggt ggaggggcct gcggaagttg cggtgacccg
      601 cgtggccccg gctcttgctc cacaaattga ccatggattc cgacgaacag gctctgcggc
      661 gtgggataat cacgatctcc cagtccgacc cactcgtcaa attgcttgat caagtaaggc
      721 ttggccggat gaaacctacc gatgccgggc tgcgtgctgt gacggcttca tggatgggca
      781 tctatcaaaa ggtgattgag tccggcggat ttaccaccca ggccttgcgc cgcatcgatc
      841 cgcaccctcg tgtggcactc ttgatcgaat ctggaatgct gacggatgac cagcaggccg
      901 tcgccgatct ccggacgagt tttgagcggg ccattgccgc tgcaacggag tgatcgcgct
      961 cttatgtggg ttctctgggg catcgccgtt ctgcttctga ccgtcggatc ggttggagaa
     1021 ggacgggctg aagaggctct cgggacgaga ccccttctgg cgattgcgcc caaggacctc
     1081 cacaccatcc tgccgcagga ctcccatatc ctcatcgagc ctcgctcact tgaacagttt
     1141 cttgattcac tggataagac cccgcccgac tgggatctgg tctacggcca tggccatgat
     1201 gaccctggac atgatgagag actatttacg ctgaaccggg agcgtgatgc aaagcgggca
     1261 ggtaactctg cgttgacaca gcgcatcacc tttgtctggt tcggtgaatt atctcggtat
     1321 gacgcggagg ctggtggatt tcgcattgca ctcggtccga ggcttacgcc tactcggtgg
     1381 ggcgtcgtgc ggttcaaatg cgaggatctt ccaagcaatc ttgtggtgaa ggtgtcatcg
     1441 tcgctccggc atgaactcga gcaacgagtt gcgcgaggcg agtcggtcga tctcacggtg
     1501 gccttgaccg gacagctcat tcctgacgaa tcagtggtgt acgacttttc gcacgaccaa
     1561 gaaggggtcg gcgtgattat gccggttgtc agggtcgaac gactcgatta tctgctgacg
     1621 aataaataaa atcggtgcag cgcaccattt tctgcatacg tactcttcct tgtttgattc
     1681 cgccaccccg gccgggcagt cctgaacgga cgcgacccac ctcccagccg ccactctgta
     1741 gggttaaaca gtcgctcctt ctcatgaccc tctgctgatg ttcgtaatat tgccatcttc
     1801 agcgtgatgc acagagagag cctgctcagg ggcattggtc ttgctccacc agcttgttct
     1861 gatgtctggg tacgagaccg ttcgacagtc gccaggaaca ggggatcggc aatgacttgc
     1921 gtcgtagctc aaggaagcat gaagaggacc aggcgacgtc agattgaatc catcagtatc
     1981 acgaagttct ttggaacggc agttgtgctg gcattcggat tccatggact tgtttccggt
     2041 atggtcggtg tcacgtctct gtatgcgtct gaggccgtga cggagtccgg acgttcgctc
     2101 atgaagtccg atagtcatcg gaacaacgaa ggccttgcga ccatcgcagg gacaaaaccc
     2161 agcctcaaat tggggatgaa gtccgattct tcgaacgtcg ggaaaaagcc ccggacgttg
     2221 atccggaaac catcatctgg tgggtctcaa accgaatctc ctcagcaggc agatcaaagc
     2281 cttccctcga gtacgcccct gggaggcggt caagctcaat ctgcgaccaa agactcagaa
     2341 tccgcgatcg cgaggtcggg agcaggcatg agttccgttg ccccaccggc gttgtcagga
     2401 agctccgtcg taaccacatc gccaggtata tcggcggctg gggcaatcgc cggggcggca
     2461 acggcatcgc ctggttcatc gatgggtgtg gctggctcgg atggatcatc ctcaggtggg
     2521 ggaagaagtg cgcagaacct gatgagacag atgccggagt tgcagcagct cctcgcaacc
     2581 cactcaggcc ctccgcctga gatgattgcg gcacccgcac caccgttggg accgccgcaa
     2641 gctggattgc caccacctcc cgcgaattgg ccggcgtacc acgtcctgaa tcgattgtca
     2701 tatggcggta cccccaatca gctcaatacc atatcccacc tcacggttca ggaggcccgt
     2761 gattgggcca ttcgctacat gaaggaacag ctggagctgg atccagacaa gccttggccg
     2821 acaaatttgc ataacacgaa cccgctcccg gctccgatcg tactcgacga ttcggcactc
     2881 agtcagcggc tggcggcgga tcgtgccgat tggcggtcag aagatggact gccggacatc
     2941 ctctcgccat tgttgagcga acttcaagat tacgatctga ttcgaaaagc ctacagccgg
     3001 cggcaactca aggagaagat ggtgtatttt tgggacaacc atttcaatac caattttcga
     3061 acgcatagca agggtcagta tgaactggct gaaaacgaat cgttcagagc gaatgcgttt
     3121 ggaaaattta tcgatctcct catggtgagt gccaagagca gcgcgatgat gatttatctc
     3181 aacaccgatg tgaatcgaaa agaaaacccg aatgagaatt acgcgcggga attgcaagaa
     3241 ttgcatacgc tcggagtcga tgtgaatggc aacccgaatg gctacatgca ggctgatatt
     3301 gtagaagccg ccaaaacctt tactggttgg acgacgttgg acatggcccg gcccggcttc
     3361 cgattcattt ccggctggca cagccca
//
LOCUS       DATIPF010000215         3922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_213660, whole genome shotgun sequence.
ACCESSION   DATIPF010000215 DATIPF010000000
VERSION     DATIPF010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213660"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..418)
                     /locus_tag="VLL06_05465"
     CDS             complement(86..418)
                     /locus_tag="VLL06_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30447.1"
                     /translation="MTDQELNRAVQYVTASTSYGRDTVAEILKTGLSEMTSLAMQSSE
                     RFERDVLLEYVCQWTIKRTGQSEPLVREVLGCASRWLDEVYEEITKRQPEAIGLSSDD
                     EDEGAEPV"
     gene            595..2418
                     /locus_tag="VLL06_05470"
     CDS             595..2418
                     /locus_tag="VLL06_05470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HSS30448.1"
                     /translation="MPVKTNRKISTSRKASSPRRQNRAYSERWDLSHLAEDPIRRFET
                     LAGAIESKVTQFESARAQLSPTMSTSTFHSLLTLSEDIAAESSRLSAYAYLWFSENTK
                     DLAARSFKTKVEERLTALHNRLVFFDLWWQSVDENTARQLMTGTGTLRYHLETIRRFQ
                     PHTLSEPEEKIVNIKNITGRSAVHSLYDVVTNSFTFTLNVNGKRKTMTREELTSYLRH
                     TQGRLREAAYRELYRVYADQHDLLGEIYKTLVNDWKAENLQLRRFASPIATRNLGNDI
                     PDDAVACLLRVCRKNAGIFQHYFRIKARLCKIKPMSRYHIYAPHRTEQKSYRYQEAVR
                     MVLDAYRGFSPQFAELAERVVHERHIDARTRPGKIGGAYCYSVVPNMTPYVLLNFTGE
                     ARDIATLAHELGHAVHGMLAARHSMFTFHSTLPLAETASVFGERILSDALMNQEQDKK
                     VRQGLLINQLDDVYATVLRQAYFVQFENQAHEMIAEGATVKDLAKTYLAELRQQFGKG
                     ITVPDEFQWEWLTIPHLFASPFYCYAYSFGNLLVLALYRTYQKEGAAFVPKYLDLLST
                     GGSEAPQTMLAKVGVDMNSEDFWQSGFDTIREMVNQLEQTL"
     gene            2590..2796
                     /locus_tag="VLL06_05475"
     CDS             2590..2796
                     /locus_tag="VLL06_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30449.1"
                     /translation="MYLCLCKGITDSDVREAGQEGIVMPGQLIAKFGLKDAGCCGRCS
                     KNIHEFVEIATNARQVPSPDGVRS"
     gene            complement(3308..>3922)
                     /locus_tag="VLL06_05480"
     CDS             complement(3308..>3922)
                     /locus_tag="VLL06_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HSS30450.1"
                     /translation="LTSIRLPFIGSLLLCALPLVLGTGAIFADEPAPRVKVLVTYHSL
                     SGNTERMAEAVVDGVKSVAGTEVLLKRVGKVTADDLFSADAVVIGSPVYWSNMSGEVK
                     TFIDNWQFKFGVFPEFKLKNKIGAAFATGGQVSSGKEVTMLTILAAMLGNQMIVISGG
                     GAFGASATTEGDSPGIDNKEIADAKALGRRVAEVAVMMQRGSSH"
BASE COUNT          940 a         1122 c         1022 g          838 t
ORIGIN      
        1 gaaagcaccg gaggcgtagc ctctgggcta cgttgaggat gctttcgagg tgagaacgaa
       61 gttcgcggac tttttcagca tcctgctata ccggctcggc tccttcatcc tcatcgtcgg
      121 acgacagacc gatggcttcc ggctgacgct tggtgatttc ctcgtatact tcatcgagcc
      181 accggctcgc gcagccaagg acctccctga ctaacggttc agactgacct gtgcgtttaa
      241 tagtccattg acagacatac tccaacagca catcccgttc gaatcgttcc gacgattgca
      301 ttgcgagcga cgtcatctca ctgagccctg tcttgagaat ctcagccact gtatcacgtc
      361 cataggatgt gctggcggtg acatattgga cggcccgatt gagttcttgg tcggtcatat
      421 ggtctcccgt gactcttggc gcatcatacc gagaatccca ccgcagatgg aagatttcac
      481 gtggttagag gcgagagaaa acttctccga ccgcccaggc caaatggtat gatcgggacc
      541 gagcacaccc tagcccgcac catacatggg ggcgagatga aggagacacg acgtatgcct
      601 gtgaagacca accggaaaat ttcgacctca cgtaaagcct catccccaag aaggcagaac
      661 cgcgcatatt ctgagcgttg ggatctctca catttggcag aggatccgat tcggcgtttc
      721 gagaccctgg caggagcgat cgagtccaag gtcacgcaat ttgagtctgc gcgggctcaa
      781 ctcagtccga ccatgtcgac atcaaccttc cactcactgc tgactctcag cgaagatatt
      841 gccgctgaat cctcccgact gagcgcctat gcctatctct ggttctccga aaatacgaaa
      901 gacctcgctg cgcgttcgtt taagaccaaa gttgaggagc gactgacggc cctgcacaac
      961 cggctggtat ttttcgacct gtggtggcag agcgtcgatg agaataccgc gcgccaactc
     1021 atgacgggaa cgggtacgct ccggtatcac cttgaaacca ttcgccggtt tcagcctcac
     1081 acgctgtctg aaccggaaga gaagatcgtc aacatcaaga acattacggg ccgcagtgca
     1141 gtccattcac tgtatgacgt cgtgacgaat agcttcacct tcacattgaa cgtcaacggc
     1201 aaacggaaga ccatgacgcg cgaggaactg acatcctatc tccgccatac gcaaggacga
     1261 ctgcgtgaag ccgcctatcg agagctctac cgagtttacg ccgatcagca cgacctgctt
     1321 ggcgaaatct acaagacctt ggtcaacgac tggaaagcgg agaatcttca actgcgtcgg
     1381 tttgcctccc ccattgccac ccgcaacctc gggaacgaca ttccagatga cgctgtggca
     1441 tgcctgctga gagtctgccg gaaaaacgct ggcatctttc agcactattt ccgcatcaag
     1501 gcccgcctgt gcaaaattaa gccaatgagt cgctaccaca tctatgcgcc gcatcgcact
     1561 gagcagaaat cctatcgtta tcaggaggcc gttcgaatgg tgctcgacgc ctaccgaggc
     1621 ttttctccac aattcgctga gctggctgaa cgggtggtgc acgaacgtca tattgacgcg
     1681 cgcacgagac ccgggaaaat cggaggcgcc tactgttaca gcgtcgtgcc gaacatgacc
     1741 ccgtacgtgc tgctgaactt cacgggtgaa gcgcgcgata tcgcaacgct ggctcatgaa
     1801 ctcggccatg ccgtccacgg catgctggcg gcacggcact ccatgtttac cttccattcc
     1861 accctcccgc tggctgaaac tgcctcggtg ttcggggaac gcatcctgtc cgatgcgctc
     1921 atgaaccagg aacaggacaa gaaggtgagg caagggctct tgatcaatca gttggacgac
     1981 gtctatgcca cggtcctccg acaagcctat tttgtccaat tcgagaatca ggcccatgaa
     2041 atgattgctg agggcgcgac cgtgaaggat ctggccaaaa cctatctggc cgagcttcga
     2101 caacagtttg gaaagggcat tacggttccg gatgaatttc aatgggaatg gctgaccatt
     2161 ccacacctct ttgccagccc cttctattgc tacgcctaca gctttgggaa cttgttggtc
     2221 ctcgccctct accggacata tcagaaggag ggggcagcgt ttgtgcccaa atatctcgat
     2281 ttactgagta ccggaggatc ggaggcaccg caaaccatgc tcgccaaggt tggagtcgac
     2341 atgaattctg aagacttttg gcaatcgggg ttcgatacga tccgcgagat ggttaatcag
     2401 ctggaacaga ccttgtaggg cctcgaaaca agagagattt tcaccttcta cctcatcaca
     2461 cctgctcctc cagtcccccc ttgacagata gatcggacgg tagtatcctt actctagctt
     2521 gataatggtt atcataatcg gtaatgacac acgagactca gtctggcgat agataaagga
     2581 gtacagccca tgtacctctg tctctgcaag ggaatcactg attcggatgt ccgggaagca
     2641 ggacaggagg gcatcgtcat gccagggcaa ttgatagcga agttcggact gaaagacgca
     2701 ggatgctgtg gtcgctgctc aaagaatatt catgaatttg tagagatcgc gacaaatgcc
     2761 cgccaagtgc cttcccccga cggagtgcga agctagcctg gttccaaagc cttccccaga
     2821 cagtcgtccc gcaaggctac cgtgacacca atccacttgc cctcaacgaa gataatcttg
     2881 tctcctgccc gcgggtagcc cgataccgcg cgctttgatt cagaaaataa caagaaggaa
     2941 acgcgagctt gtaggtaggg accaccactg actaataggg agttgaaaaa ctaatttcga
     3001 actaaagaga tacaagtctg gaggaagttc ttgacggctc cggctagacc agcagactgt
     3061 tcaaaaaggt catccggcaa ggccgcagcg agtgaaggcc cgaggaggta taaaccgcac
     3121 ttcgtgtgag ccgttcgccc ttgcaatgga ttttggcgaa cggaaaagcc cctccagcgt
     3181 ttccgatctc cgagaatctc attcggtacg ttgagtgtct gaacgatgcg agaacgccgc
     3241 cagggaagag gcgcgtcttg gcacgccggg gttgggtggt gagaagaccg actttttcaa
     3301 cagcctgcta gtgagaagag cctcgctgca tcatcacagc gacttcggcg acgcgccggc
     3361 ccaatgcctt ggcatcggct atctccttat tgtcgatgcc ggggctgtcg ccttccgtgg
     3421 tggcagatgc gccaaaggcc ccacctccgc tgatgacaat catctgattt cccaacatcg
     3481 cggcaaggat cgtcaacatc gtcacttcct tcccactcga cacctgaccg cctgtggcaa
     3541 aggccgcgcc gattttgttc ttcaacttga attctgggaa cacaccgaat ttaaactgcc
     3601 agttgtcgat gaatgttttg acctcacccg acatattcga ccagtagacc ggcgagccga
     3661 tcacgaccgc atcggcggag aacaaatcgt ctgctgtcac cttaccgact cgtttcaaca
     3721 acacctcggt gccggccaca cttttcacac catcgaccac cgcttcggcc atccgttccg
     3781 tattaccgga cagtgaatgg taggtcacga gcacctttac ccgcggagcc ggctcgtcgg
     3841 cgaatatcgc gccggtgccc agcaccagag gaagggcaca cagcaacagt gaaccaatga
     3901 aggggagtcg tatagaggtg ag
//
LOCUS       DATIPF010000216         7475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_40659, whole genome shotgun sequence.
ACCESSION   DATIPF010000216 DATIPF010000000
VERSION     DATIPF010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7475
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40659"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1834)
                     /locus_tag="VLL06_05485"
     CDS             complement(119..1834)
                     /locus_tag="VLL06_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HSS30451.1"
                     /translation="MSTVSPSSEQQLDRAALAALYEETFKNLEEGTITEGRVVAVTKD
                     KVIVDIGYKSEGMIPNDQFSTEELQNIKVDDRFQVYIEECEDADGNLVLSKEKADKMK
                     IWEELEKLFNDGKSIDGKIVARIKGGMMVDIGVKAFLPGSQIDLHPVRDLDGLVGRTF
                     PLKIIKINHRRGNVVVSRRVLLEETRDSKRKTTLSTLKEGQLIQGVVKNITDYGAFID
                     LGGIDGLLHITDMSWGRVGHPSEMFNIGDKVEVSVLKYDRETGRISLGLKQKSADPWT
                     GVASKYAIGTRVRGRVVSLTDYGAFIELEPGVEGLVHVSVMSWTHEVRHPSRVVSIGD
                     QVEAAVLNVDPASRKISLGMKQTAPNPWDMVEGKYAIGTRIEGKVKSLTDFGAFVGLE
                     EGIDGLIHISDMSWTKHIKHPSELFKKGQKVEAVVLRIDKEKERLSLGYKQLKSDPWD
                     DEIPNRYGVGDVAVGKVSKVADFGIFVELDGGVEGLIHISEAGLDPQAKLEEKFKLMD
                     EVTAKIIKVDREERKIALSLRDHEMDTDRRKVDEYHAAQGALDQSLGRAAKQNRKRGQ
                     DEDDK"
     gene            complement(1891..2547)
                     /locus_tag="VLL06_05490"
     CDS             complement(1891..2547)
                     /locus_tag="VLL06_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSS30452.1"
                     /translation="MSSVVYGILWILCRTIGWLCFRFRVVGEVPKTGGFIIAANHASY
                     LDIPLLGCGMRRGAWYLGRSDLFPVPGLRAICQWLGWIPLRTGRVDRDAFGKAVTLIK
                     EGKVVVIFPEGSRSQDGRLQSPKPGIGVIVAQTGCPVVPAYLKGTFDVLPSGAMWPRF
                     RPVTVFYGDSITFPRIEEQEEVKQFYQEVSRTVMDRIASLGAVESPAQQSRAGIRNAE
                     "
     gene            complement(2544..3239)
                     /gene="cmk"
                     /locus_tag="VLL06_05495"
     CDS             complement(2544..3239)
                     /gene="cmk"
                     /locus_tag="VLL06_05495"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HSS30453.1"
                     /translation="MGSIAQEKQASRLIVAIDGPAGVGKSTVARLLASRLGYLYLDTG
                     ALYRAMAWAVIESGRDSGDVEAVAALLPTLSLHMQFHHGTATVLVNGKDVTGDLRTPE
                     VSAAASVVSAIPAVRAWLLPIQRQIGQGGAVVAEGRDMGTNVFPAADVKFFLEADPTV
                     RAQRRHRELVAAGHSRALEETSADLAGRDTRDRSRSIAPLTPAEDARFIDTSTLSIAE
                     VVDQMMVVIAARL"
     gene            complement(3263..4585)
                     /gene="aroA"
                     /locus_tag="VLL06_05500"
     CDS             complement(3263..4585)
                     /gene="aroA"
                     /locus_tag="VLL06_05500"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HSS30454.1"
                     /translation="MASLSITPGRPLLGTISVPGDKSITHRAIILAALANGVSTIARY
                     GRGEDCLNTMRAFQALGIRIDESVEELRVYGKGLWGLTEPSGLIDCGNSGTGIRLLTG
                     LLAAQDFFTVLTGDDSIRRRPMGRIVKPLREMGATIAGRKGGELAPLAVMGGRLRAIT
                     YASPVASAQVKSALLLAALFAEGTTRITEPRLSRDHTERLYQFLGIGLRREGATLLID
                     GRPSVGWNAAPRLVVPGDFSAAAFFIVGATIVPGSDVTIRQVGINPTRTGLLGVMTRM
                     GADIQLLNQREEAGEPVADIRVKSARLRGVEIGPDLIPQTIDEFPILCVAAAVAEGKT
                     IISGAEELRVKESDRITTVSAELRAMGAQITEHPDGMVVRGLGTAEENGRLQAGRGRS
                     HGDHRVAMSLAIAGLTASASTLIDETMCIETSFPAFHHTLLELLTERR"
     gene            complement(4591..5484)
                     /locus_tag="VLL06_05505"
     CDS             complement(4591..5484)
                     /locus_tag="VLL06_05505"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HSS30455.1"
                     /translation="MALHFTQVTIIGVGLIGGSLGMILRRRGLAERVIGVGRRVENLK
                     TAVELGAIDRYVVDPKEGVRDADLVVLATPVDTYERHLTEWASGLKKGTIVTDVGSVK
                     GLLVEQAERAMPAGVHFVGAHPIAGKEKTGVAAGSDQLFMGARCIITPTNTTDPQALE
                     QVRTMWQETGSVVLTMDAHLHDKILGAVSHLPHVAAFALINALIEIRDQQIPSLDLAS
                     HSGGGLRDTTRIAASSSEMWRDIFLWNRDNVVTFIEAYERSLSQLKQLIRMGDAGGIE
                     KELERARQEREKLSTRLAASA"
     gene            complement(5545..6558)
                     /gene="aroF"
                     /locus_tag="VLL06_05510"
     CDS             complement(5545..6558)
                     /gene="aroF"
                     /locus_tag="VLL06_05510"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HSS30456.1"
                     /translation="MIIVLKPEASEREVDHIIDRLRELGLKSQLSTGQERTIIGVVGD
                     DRILHNQPLTAMPGVESVLPILAPWKLVSREFKREGTMIDVSGMKIGGNKLIIMAGPC
                     AVERLEITVGIAHEVKAAGASILRGGAYKPRTSPYSFQGLGREGLDYLVEARKQTGLP
                     VVSEILDTRDIELFLEKADIIQIGARNMQNFELLKEVGAYDKPVLLKRGLSATIKEFL
                     LSAEYIMSRGNQNVMLCERGIRTFETQYRNTLDLAAVPTLKELSHLPVIVDPSHATGK
                     WNLVAPMSKAAVAAGADGILIEVHSNPECALCDGEESIKPSQFKTLMQDMRKIAVAVG
                     REL"
     gene            complement(6577..>7475)
                     /gene="hisC"
                     /locus_tag="VLL06_05515"
     CDS             complement(6577..>7475)
                     /gene="hisC"
                     /locus_tag="VLL06_05515"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HSS30457.1"
                     /translation="YPDGGAFRLREALADRWKVTPDQVILGNGSDEILGLLARTFLAP
                     GDEAIMADQTFIIYKMEVTAAHGKPIIIPLKQWRHDLQTMADAITGRTRLLFLCNPNN
                     PTGTMVPADEVERLLLRVPAHVVVVFDEAYFEYVRSQQFPDSMAYVKQGRNVIVLRTF
                     SKIYGLAGLRIGYGVATAEITNLLNRVRPPFNANSLAQRAALAALDDDEHVARSRAVN
                     ATGMEQMVKGLSALGFAPIPSEANFVYVDVGREGRTLFEAMLREGIIVRHIEGPMVRV
                     TVGLEEENREFLAALKRVIHSH"
BASE COUNT         1605 a         2391 c         1854 g         1625 t
ORIGIN      
        1 gcgcacgatc cctcgcttgg ccggtctttc tcttgtgtcg tcgctcatag ttctcttcgc
       61 tcacagtgcc gggggcgcga acgccctccg gcttacactg tgacctacta aatcggagct
      121 acttatcgtc ctcgtcctgg cctcgcttcc gattctgctt cgccgcgcga cccaaacttt
      181 ggtccaacgc gccttgcgca gcatgatatt catcaacctt ccggcggtct gtatccattt
      241 catggtcgcg caggctgaga gcgatcttcc gttcttcacg atcgaccttg atgatcttgg
      301 ccgtcacttc atccatcagc ttgaatttct cttcgagttt ggcctgcggg tcgagaccag
      361 cctcgctgat atgaatcagc ccttcgaccc ctccgtccaa ttcgacgaag atgccgaagt
      421 cggcgacctt actcaccttc ccaactgcca catccccaac cccataccgg ttgggaatct
      481 cgtcgtccca tggatcgctc ttgagctgct tatacccaag cgacagccgc tccttttcct
      541 tgtcgatccg caaaacgacg gcctcgacct tctgcccttt cttaaagagt tcagagggat
      601 gcttgatatg tttcgtccaa gacatgtcgg aaatgtggat caagccatcg atgccctctt
      661 cgagtccgac gaacgcgcca aagtcagtga gactcttcac cttcccctcg atgcgcgtgc
      721 cgattgcata cttcccttcc accatatccc agggattcgg cgccgtctgc ttcatgccga
      781 gcgaaatctt cctgctggcg ggatccacgt tcagcaccgc cgcttcaacc tgatccccga
      841 tggacacgac gcgtgacgga tggcgcacct catgtgtcca tgacatcacg gaaacatgca
      901 ccagtccctc gacgccgggc tcgagctcga tgaacgcccc atagtcagtc aggctcacga
      961 cacgacctcg gacgcgggta ccaatcgcat atttactcgc cacgcccgtc cagggatcgg
     1021 cactcttctg tttcaagcca agggaaatgc gcccggtctc ccgatcatac ttgaggacgc
     1081 tcacttccac cttatcgccg atgttgaaca tttccgacgg atggccgaca cggccccatg
     1141 acatatccgt aatgtggagg agtccgtcga taccgccgag gtcgatgaat gctccataat
     1201 cggtgatgtt tttcaccacg ccttgaatca attgcccttc cttcaacgtc gagagagtcg
     1261 tcttccgttt cgaatctctc gtctcctcca acagcacccg ccgagacacc acgacgttgc
     1321 cgcgccgatg attgatcttg ataatcttga gagggaacgt gcgccccacc aacccgtcaa
     1381 ggtcacgcac gggatgaagg tcgatctgcg agcccggtag aaacgccttc acgccgatat
     1441 ccaccatcat gccgccttta atcctcgcca cgatcttgcc gtcgatgctt ttgccgtcat
     1501 tgaagagctt ttccagctct tcccaaatct tcatcttgtc ggctttctcc ttggagagaa
     1561 ccagattgcc gtcggcgtcc tcacactctt cgatatagac ttgaaaccgg tcatcaacct
     1621 tgatgttctg aagttcctcc gtggagaact gatcgttcgg gatcatgcct tcggatttgt
     1681 agccgatgtc gacgatcacc ttgtctttgg tcacggcaac gacgcggcct tccgtgattg
     1741 taccctcttc gaggtttttg aatgtctcct catagagcgc cgctaaagcc gctcggtcta
     1801 actgctgttc acttgatggc gaaaccgtac tcatggtgtg aatcctactc ttccgacagg
     1861 agtcgggtgc tgatggttat acaccggcat tcactcagcg ttgcgaatgc cggcacgaga
     1921 ttgctgggcc ggagactcta cggcacctaa ggaggcgatg cgatccatca cggtacgact
     1981 gacctcctga taaaactgct tgacctcttc ctgttcctcg atgcggggaa acgtgatgga
     2041 atccccatag aaaacggtca cgggacggaa gcgcggccac atagccccag acggcagaac
     2101 gtcgaatgta cccttcagat aagccggaac cacaggacat ccggtttgag ccacaatcac
     2161 accaatcccg ggcttagggg attgtaaacg gccatcctgg ctgcgactgc cctctggaaa
     2221 gatcaccacg acctttcctt ctttgatcag cgtcactgcc ttaccaaatg cgtcacgatc
     2281 cacccgaccg gtccgcaggg gaatccatcc caaccattgg cagatggccc taaggcctgg
     2341 aaccggaaag agatcgctac gccccaagta ccaggccccc cgccgcatcc cacaacccag
     2401 gaggggaata tccaggtaac tcgcatggtt cgcggcgatg atgaagccgc cggtcttggg
     2461 aacctcccca acaactcgaa agcgaaaaca gagccagccg atggtccggc aaaggatcca
     2521 caggatcccg tacacaaccg aactcacaat cgggccgcaa tcacaaccat catctggtcc
     2581 accacctccg cgatggacaa cgttgaggtg tcaatgaacc gggcatcctc agcgggagtc
     2641 agcggagcga ttgaacgtga acgatctcgt gtatcgcgtc ccgccaggtc tgctgacgtc
     2701 tcttcaagtg ccctggaatg gcccgccgca acaagttccc gatgtcggcg ttgggccctg
     2761 acagtcggat ctgcttcgag gaaaaacttc acgtcggccg cagggaacac attcgttccc
     2821 atatcccgcc cctcagccac caccgctccg ccttgtccga tttgccgctg gataggcagc
     2881 aaccaggccc ggaccgcggg aatcgccgag acgaccgatg ctgccgctga tacctcaggc
     2941 gttcggagat caccggtcac gtccttgcca ttgaccaaca ccgttgccgt tccgtgatgg
     3001 aactgcatgt ggagcgaaag ggtgggtaac agcgcggcaa ccgcctcgac atcccccgaa
     3061 tcccgtccag actcaatgac agcccaggcc atcgcacggt agagggctcc cgtatcgaga
     3121 tacagatagc ccaatcgaga tgccaacaac cgggccacag tactcttgcc gacaccagcg
     3181 ggaccatcga tcgctacaat gagccggctt gcctgcttct cctgcgcaat agatcccacg
     3241 tcgtggcccc gtattatagc cgtcaccggc gttcagtcaa taattccaag agtgtgtgat
     3301 gaaacgctgg aaatgatgtc tcgatgcaca tcgtctcgtc gatcagcgtt gatgccgaag
     3361 cggtcaagcc tgcaatggcc aatgacatcg ccactcggtg atctccatga ctgcggccac
     3421 gtccagcttg taatcgaccg ttttcttctg ctgtgccaag tcctcgtaca accatcccgt
     3481 ctggatgttc tgtgatctgc gcccccatcg cgcgcaactc cgccgacacc gtcgtaatcc
     3541 gatcgctctc tttgacccgc aattcttcag ccccggagat gatcgtctta ccttctgcaa
     3601 ccgcagctgc cacacaaagg ataggaaatt catcaatcgt ctgtgggatc aagtccggcc
     3661 cgatctccac cccacgcagc cgtgccgact tcacccggat gtccgccacc ggctcgcctg
     3721 cctcttcccg ctgattgagc aattgaatat ccgcccccat tcttgtcatc acgcccaata
     3781 aacccgtcct ggtcgggttg atccccacct ggcgaatcgt gacatccgag ccgggaacaa
     3841 tcgtggcgcc cacaatgaaa aacgcagcgg cagagaagtc acccggcacg accaatcgtg
     3901 gcgccgcgtt ccacccaacc gacggtcgtc cgtcgatcaa caaggtcgct ccttcccgtc
     3961 gaagcccaat acccagaaac tgatacaacc gttctgtatg gtcacgcgaa agacgcggct
     4021 ctgtgatgcg ggtcgtccct tcggcaaaca gggctgcgag caacaaggcc gacttcactt
     4081 gtgcgctcgc cacaggagag gcgtaggtga tcgcccggag acgccccccc atcacggcta
     4141 agggtgcaag ttcaccaccc ttacgcccgg cgatggtcgc ccccatttca cgcagcggtt
     4201 tgacgatccg ccccattgga cggcgacgaa tcgaatcgtc gccggtgagt accgtaaaaa
     4261 agtcctgtgc cgccagcagg ccggtcagga gacgaatacc agtcccggag ttcccgcaat
     4321 caataagccc actcggctcg gtcagacccc agagtccctt cccatacaca cggagttctt
     4381 cgaccgattc atcgatgcgt atcccgagag cctggaaggc gcgcatcgta ttgagacaat
     4441 cttcgcctcg accatagcgt gcaatcgtgc tcaccccatt cgccaaggct gccaggatga
     4501 tggcacgatg ggtgatcgac ttatcgccag ggacggaaat cgttcccagt aacggtcggc
     4561 ctggtgtgat agacaaagat gccatgggtg ctatgcggac gccgcgagtc tcgtggaaag
     4621 cttttcacgc tcctgtctgg ctcgctcgag ttccttttca atacctccag catcgcccat
     4681 tcgaataagc tgtttcagct gactcaacga acgttcatac gcttcaatga acgtgaccac
     4741 attgtcacgg ttccagagaa aaatatcacg ccacatttcc gaggaactcg cggcaatcct
     4801 ggtcgtatcc cgcaatccgc cacctgaatg gcttgccaga tcgagagagg gaatctgttg
     4861 atcgcggatc tcaatcaggg cattgatcaa tgcaaaagcc gccacatgag gcaaatggct
     4921 caccgctcca agaatcttgt catggagatg ggcatccatt gtgaggacca ccgaaccggt
     4981 ttcctgccac atcgttcgaa cctgctcaag tgcttgggga tccgtcgtgt ttgtcggagt
     5041 gataatgcag cgcgcaccca taaagagctg atccgatccg gccgccaccc ccgtcttttc
     5101 ctttccggca atcggatgag ctccgacgaa atggacaccg gccggcatgg ctcgttcagc
     5161 ctgttccacc agcaaaccct tcacgcttcc gacatcggtc acaatcgtac cctttttcaa
     5221 accggaggcc cactctgtca ggtgccgctc ataggtgtcg actggggtgg ccaacacgac
     5281 taggtcggca tccctcactc cctctttggg atcaacgacg tagcgatcga tcgcgcccaa
     5341 ctcgaccgca gttttcaagt tttcgacacg cctcccaacc ccgatgaccc tttccgccaa
     5401 cccccttcga cggagaatca tacccagcga gccgccgatc agccccactc cgataatcgt
     5461 gacctgtgtg aaatgcagtg ccatttttca cgctggaccg aggccaaccc tcaatcctga
     5521 atccctcacg caccgtgtat ctgattacaa ttcccgaccg accgccacgg caatctttcg
     5581 catgtcctgc atcaatgtct tgaactgact cggtttaatg gattcttcac cgtcgcacag
     5641 cgcacattcc ggattcgaat ggacttcgat cagaatgccg tcggcccccg cagccactgc
     5701 agctttcgac atcggcgcca ccagattcca cttaccagtg gcatggctgg ggtcgacgat
     5761 gacgggcaaa tgcgacagtt cctttaacgt cggaaccgct gcgagatcga gcgtattgcg
     5821 atattgcgtc tcgaaggtcc tgatcccccg ttcgcacaac atgacgttct gattgccgcg
     5881 ggacatgata tattctgccg acaggagaaa ttccttgatc gtcgccgaaa gtccccgttt
     5941 caacagtaca ggtttgtcat acgccccaac ctccttgagg agttcaaaat tctgcatgtt
     6001 gcgggcgccg atctggataa tgtcggcctt ctcgagaaat aattcgatgt cgcgggtatc
     6061 cagaatctcg ctgaccaccg gcaatcctgt ttgtttcctc gcttcaacca gatagtctaa
     6121 tccttcacgc cctaatccct gaaaggaata gggagaggta cgtggcttgt aggcgccacc
     6181 gcgcaaaata cttgctccgg ccgccttgac ctcgtgggca atgccgaccg tgatctcgag
     6241 tcgctccact gcgcagggcc ccgccatgat gatcagctta ttgccgccga tcttcatgcc
     6301 gctcacatcg atcatagtgc cttcacgctt aaactcccgg ctgacgagtt tccagggcgc
     6361 gagaatcggc aacacacttt caacacctgg catggccgtg agcggttggt tatgcaggat
     6421 ccgatcgtcg ccgacgactc cgatgatcgt ccgttcctgg ccggtcgaga gttgtgactt
     6481 caagcccagt tcgcggagcc gatcgatgat gtggtcgact tcccgttcac ttgcctctgg
     6541 tttcagtaca atgatcatat tgtcctcacc atacactcaa tgcgaatgga tcacccgctt
     6601 gagcgcagcc aagaattccc tgttttcctc ttccaggccg acggtcactc gaaccatagg
     6661 cccctcgatg tgacgaacga tgatgccttc cctcaacata gcctcaaaca acgtgcggcc
     6721 ctctcgcccg acatcgacat agacaaaatt cgcttcgctc gggatcggcg caaacccaag
     6781 ggcggacaat cccttgacca tctgctccat ccccgtcgca ttcacggctc gactccgtgc
     6841 cacatgttcg tcgtcatcga gagcggcgag ggcggcgcgt tgtgccagac tgttggcgtt
     6901 gaagggaggg cggactcggt tcaagagatt ggtgatctcc gctgtcgcca caccgtaccc
     6961 gattcgcaat ccagcaaggc catagatttt cgaaaacgtt cgcaacacaa tcacgttgcg
     7021 cccctgcttc acataggcca tggagtctgg aaactgctgg ctccggacat actcgaaata
     7081 ggcttcgtcg aacacgacga cgacatgcgc cggcacgcgc aacagcagac gttcgacctc
     7141 atcggccggc accatcgttc cggttggatt attgggatta cagaggaaca ggagccgtgt
     7201 ccggccggtg atggcgtccg ccatggtctg aagatcgtgc cgccactgct tcaatgggat
     7261 gatgataggc tttccgtggg ctgccgtgac ctccatcttg tatatgatga aggtctgatc
     7321 ggccatgatc gcttcatcgc ccggagccaa gaacgtacgg gcgagtagac ccaagatttc
     7381 atccgatcca ttcccgagaa ttacctgatc cggagtcacc ttccaacgat cagccagggc
     7441 ttcgcgtaag cgaaatgcgc cgccgtcggg atagc
//
LOCUS       DATIPF010000217         9518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_307689, whole genome shotgun sequence.
ACCESSION   DATIPF010000217 DATIPF010000000
VERSION     DATIPF010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 9518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9518
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307689"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            406..1182
                     /locus_tag="VLL06_05520"
     CDS             406..1182
                     /locus_tag="VLL06_05520"
                     /inference="COORDINATES: protein motif:HMM:NF023899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HSS30458.1"
                     /translation="MRSAHWSLPPDQTLLYLTLGAGGGVGLFIYGWVVHQRKRLIESI
                     PTSTIRSLALGLVEISGTAQSKEGFLSTPFSGLPCVFYAYAVEERVGSGKNARWETIV
                     KGTSEQPFLVRDATGQVLVVPLGAELILPDERTSRSSWLGELPPTTIAGLNRLGISTE
                     RWMGSKTLQCRESFILPEEQVYVLGTAQEHLGASGHSENSSPLYIGGSQDREFIISDR
                     SEQELLSKLQWQILAFWVGGLTLAVICLIIIFEHYLTTMS"
     gene            1291..1797
                     /locus_tag="VLL06_05525"
                     /pseudo
     CDS             1291..1797
                     /locus_tag="VLL06_05525"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
     gene            complement(1870..4491)
                     /locus_tag="VLL06_05530"
     CDS             complement(1870..4491)
                     /locus_tag="VLL06_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSS30459.1"
                     /translation="MTIPLFLPIALIRAHALQRPFRALLSIAGVALGVLASVAIGTAN
                     MQVLRSFEQAVTTVAGPATLEIAGHELGVDESVITAVRAVEGVVSAAPVIEEAIVVAQ
                     GKSPGQTLEILGLDLLAEARTRGFQILEAETEAALEALLAPDALYLGRQVAADWNLGV
                     GSIIEGTVGGRLIRLRVVGLIHNEAARSSLWDRLAVMDIAAAQLLFQSIGRLDRIELV
                     TTPDRPLDDIAASLRTVLPPHLVVQRPTQRTKQVENMVWAFQLNLAIMSWVGLLVGMF
                     LIYNTIAFVVTQRRREIGIYRALGMTEWRVAGLFLMEAGLLGLLGGLLGGLGGIWLAR
                     SLVSLVSRTISDLYVPITSGGLILSMDMLTFAAVAKGVLLGTVVSMVGALGPSVEAAR
                     TVTVRALAPGDYESTHRLRAGLCGWISLVLIALAGLCSLMGPVGDLPLFGYIATLCVL
                     GSLSFLAPLCIQALGMRRPRQEGQMMMMLGGSLRHIAADQAARHPGRNSVTVSALMVG
                     LSIMIGVAVMVRSFRDTVEVWVDETVMADLVVAPQAWLQGKQVGQASSALPGTWRSIL
                     STTEGIAAVDAIREVSVDVDGQPVALVSRDFRLHAQRSRYRMIHGDSSTALRRAAETG
                     GVLLSEVLATRLRLHEGSKVSITTPAGAITVSVEGVFYDYATDGGKMVIDRTWYQREW
                     HDDRVSVFPIYLDAGADAEQVRQSIVTHITGLDGTTVPPLVIRNHELRKEILEIFDRS
                     FVLTYVLEAIAVLVAVLGIINTLVTAVLERRREFATLRAIGAGARQVERLVLWEAAYL
                     GLIGAVLGVVGGLLLALLLIHVVNKQSFGWTIQMTVPGGLILQAVGLALTAALVAGYF
                     PARWAARQPLVEGLREE"
     gene            4771..5043
                     /locus_tag="VLL06_05535"
     CDS             4771..5043
                     /locus_tag="VLL06_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HSS30460.1"
                     /translation="MGARTALNRRIQPLQEDIDADLLTPRQRDILRLVSIGHTNREIS
                     EVLAISVRTVEVHRFNLMRRLNVRNVAQLLRRALQLGLLAKTFGAK"
     gene            complement(5046..5972)
                     /locus_tag="VLL06_05540"
     CDS             complement(5046..5972)
                     /locus_tag="VLL06_05540"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HSS30461.1"
                     /translation="MDLSTTIAGVRFPSCFMNASGALCVTREELLVLGRSRAGAIVTK
                     SMTVEPRDGNPTPRYYGFSGGSINSMGLPNLGYKAYAALIPELKSLGKPVIASVAGLC
                     EDDFPTIATTINAAKPDLIEVNLSCPNIPGKPQIGYDAEASERLLKRVRKVITVPMGV
                     KLPPYFDPAHHAVMGEVLLRCGVDFLNLINSVGNGLVVDPEREVVLIKPKGGFGGLGG
                     TIIKPVALANVRAFWKIFEGRMPIIGTGGVVNGMDAFEHLLCGASAVQIGTVLVEEGV
                     DVFGRLETELTTQLSKKGYTKVEDCRGKLKEL"
     gene            complement(5972..6676)
                     /locus_tag="VLL06_05545"
     CDS             complement(5972..6676)
                     /locus_tag="VLL06_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSS30462.1"
                     /translation="MTMVSFDQVSKIYERGGDTITGLDRVTVEVAKGDFCAFIGPSGC
                     GKSTLLNLVAGLDVPTSGDILLGGRSTSRFTSNDWTRIRRETVGIIFQAFHLIPGLTA
                     AENVAFPLLLRGERGGTVQARVEEMLEVVSMTSRRHHRPSELSGGEQQRVAIARAIVH
                     QPEILLADEPTGNLDSQHGAEIIVLLRSLVQRFRQTVLLVTHSTAAAEAADYVWAMKD
                     GQLVSRTQHAPVTEVA"
     gene            6901..7722
                     /locus_tag="VLL06_05550"
     CDS             6901..7722
                     /locus_tag="VLL06_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HSS30463.1"
                     /translation="MLREGTQDRLIGWCRQALRFVLPVECCACGHSLGTDPVPFFCTA
                     CWHTIRPLQQPVCARCDQPFASPAATTYTPNHQCQQCQEQPPEFQRAWTLFPYLSPLR
                     EAICSFKYRGKHTLARPLARLMISALPSGIDIDVIVPVPLHPTRLRAREFNQSLLLAD
                     QLSRHLVRPVSPTHLVRIAATDPQTTLTRQARLRNLRKAFEVRKPQDLVEKRILLVDD
                     VFTTGTTLNECAKTLRKAGSGPVFALTLARTVDTSLIPDRHFAERTLPTLTTIGI"
     gene            7726..8070
                     /locus_tag="VLL06_05555"
     CDS             7726..8070
                     /locus_tag="VLL06_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HSS30464.1"
                     /translation="MPLFEYRCLDCKKRSTVLVLSQANQAPAACAHCTSARVERILSR
                     FASPKSEAARLEALADPSSLAGLDENDPQSMARFMETMGEEMGEDLSDDVSAAMESDG
                     TDGMESDGTDRL"
     gene            8181..8378
                     /locus_tag="VLL06_05560"
     CDS             8181..8378
                     /locus_tag="VLL06_05560"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSS30465.1"
                     /translation="MAREPKSELMTAEETCRYLKITQRTLYRYLQSRQIPAFKLGKEW
                     RFVRSDLEQWIRDRTRATLAS"
     gene            8395..8835
                     /locus_tag="VLL06_05565"
     CDS             8395..8835
                     /locus_tag="VLL06_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30466.1"
                     /translation="MFAGEYLCKVDEKGRFIVPSPIREQVESEGQAVVFLKGPEESTL
                     IYSMKEWEKVLERTKTTLDEDQSRLFMHFVVSEAGTSEIDKTGRILIPSRLRKIIPLD
                     EDQEIVLVGLYHRMEIWNPSTWRRYISQSEERYEQNMSKILNLL"
     gene            8936..9397
                     /locus_tag="VLL06_05570"
     CDS             8936..9397
                     /locus_tag="VLL06_05570"
                     /inference="COORDINATES: protein motif:HMM:NF017663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HSS30467.1"
                     /translation="MLRIAPRRAPSSSIPTSPLPHPPAEGHAVHLTLPVPPSVNHQYA
                     TVNGRRLISSAGRAYKAQVGQLVWLKLSQSTQRAALLTRLQSDWLALSIRFYFTSALR
                     RDVDGGLKIAQDALCEGLGINDNRIVETHLYKHIDKHNPRIEVHLTPAAGS"
BASE COUNT         2209 a         2918 c         2456 g         1935 t
ORIGIN      
        1 gtggaggact accgctcgtt gaaccgggct cttggtgata tcggaggtgt cacggtcaga
       61 acagagacat tgtgtccccg atgcggcaat cacatggagg ctgagacagt tgttcacacg
      121 tccttcctga gctgcaaatc ctgtcacggc tggtggcttc ctcacggaag cctcactcat
      181 ctctatgaga cctatcgagg ctccgcggtt ccgatccagc tccacgaagc cgagatgtat
      241 atacgagcag acgctagact tcgtgcacac aacgaaacaa tgcacgatca acccaggatc
      301 aatacaacac gcgccaagcc acagagcctc tggttttggg tcctgttctt cggattcgct
      361 tttgtactag gcggtctcat cctctttgcg gggattggta aagccatgag atccgctcac
      421 tggagcctac cgccagatca gaccctgctg tacctgacat taggtgctgg aggaggtgtt
      481 gggcttttca tctatggatg ggtggttcac caacgaaagc gcttgatcga aagcattcca
      541 acctcgacta ttcgttcgct cgctctgggg ttggtcgaaa tcagtggaac agcacagtcc
      601 aaggagggct tcctttctac gcccttcagt ggacttccct gtgtgttcta cgcgtatgcc
      661 gttgaggaac gggtcggctc aggcaagaac gcacgatggg aaaccattgt gaaaggcact
      721 tctgagcaac ctttccttgt gagggatgca accggacaag tactcgtcgt gcctcttggt
      781 gccgagctta tcttgccgga tgagcgcacc tctcgcagta gttggctcgg agagttaccc
      841 cctaccacaa tcgccggctt aaatcgattg gggatttcaa ccgaacgttg gatggggagc
      901 aagacattac aatgccgaga atcgtttatt ctgccggagg agcaggtcta cgttctcgga
      961 accgcccagg aacacctcgg tgcaagcggg cacagcgaga actcctctcc cctctacatc
     1021 ggtggtagcc aagaccgcga attcatcatc tcggaccgaa gcgaacagga gctattgtcc
     1081 aagttgcagt ggcaaatatt ggccttttgg gtaggaggcc tgacgcttgc tgtaatctgt
     1141 ttgatcatca tcttcgagca ctacctaacg accatgtcct aatggtcata cccacaaagg
     1201 agaacgtcca tgaatggtac cttgctgatt gggattctgc tggttactgg actggtcata
     1261 cttattgcct atgttgccgg cgcctacaac atgctggtca ggctcgccaa caacatcgac
     1321 aaggcttggt cgaatatcga cgtgatcttg aaacaacgtc atgatgagct tcccaagctt
     1381 gtccaggtct gtaatagcta catgacgcac gagcgggaaa ccctggaatc cgttaccaat
     1441 gcccgaagtg cgtatagcgc aggccggaac atcaacgaca aggcacaggc agagaatcaa
     1501 atcgtcgatg ctttagggaa actgtttgct gtggctgaac agtagccaga tctgaaagcc
     1561 aatcaagaat tcttaactat tcagcagcgt atatcggcgc tggaaaacac tattgcagac
     1621 cggcgcgagt tctataacga ctcggtcaat ctctataaca ttgccatcga acagatccct
     1681 acgctctggg tggctcagca ggttggatac acagctaggc cattgctgac agttgcacca
     1741 tccgatcgca aggatgcgcc gctcgatttt gccaatcgta cacgtatggc cgcgtagaca
     1801 cagactcggg gcccattgtg gtgttcggtg agacggacgg aatgaaagag cgaccttggc
     1861 ctcttccttt tattcttccc tcagcccttc aaccagcggc tgccgtgcgg cccagcgggc
     1921 tgggaagtag cctgccacca gagcagctgt gagtgcgagc cccacggctt gcagaatgag
     1981 tccgcctgga accgtcatct gaattgtcca accaaaagat tgtttattga ccacgtggat
     2041 aagcagcagg gcgagtagca accctcccac gactcccagc acagccccga ttaacccgag
     2101 atacgcggct tcccataaca ccaatcgttc cacttgcctc gcgccggcgc cgatagcccg
     2161 caaggtggca aattcccttc gtcgctccaa cacggccgtc accaaggtat tgatgatgcc
     2221 aagcacggcc accagcactg cgatagcttc aaggacatag gtcagcacga aggagcggtc
     2281 gaagatctcg agaatctcct tccgcaactc gtggtttctg atcaccaacg gtgggactgt
     2341 cgtgccatcc agaccggtga tatgcgtaac aatcgactgc cgaacctgtt ctgcatcggc
     2401 cccggcatcc agatagatgg gaaacaccga cacccggtcg tcgtgccact ctcgctgata
     2461 ccatgtgcga tcgatcacca tcttcccacc gtcggtcgcg taatcgtaaa acactccctc
     2521 gacggacaca gtaatggctc ctgccggcgt cgtgatcgaa accttgcttc cctcatgaag
     2581 ccgtaaccga gtcgccaaca cttccgacag gagaacgcct ccggtctcgg cggctcgccg
     2641 cagagccgta ctcgaatctc catgaatcat ccgataccgg ctgcgctgtg catggagccg
     2701 gaaatcacgt gataccaacg cgactggttg accatcgacg tccaccgata cctcccgaat
     2761 ggcatcgact gcggcgatac cttcagtcgt cgagaggatc gaacgccagg ttcctggtaa
     2821 ggcactcgac gcttgcccga cctgcttacc ctggagccag gcttgcgggg ctacgacgag
     2881 atcggccatc acggtctcat cgacccagac ctcgaccgtg tcacgaaaac ttcgcaccat
     2941 aacggccaca ccgatcatga tcgacaatcc caccatcaac gccgacaccg tgactgaatt
     3001 ccgacccgga tgacgcgccg cctggtcggc tgcgatatgc cgaaggctcc ccccgagcat
     3061 catcatcatc tgcccctcct gccgaggacg tcgcataccc aacgcttgga tacagagtgg
     3121 cgcaagaaag gagagtgatc ctaagacaca gagcgttgcg atatagccga aaagcggaag
     3181 gtctccgacc ggacccatca gcgagcagag gcctgcaagc gctattaaca cgaggctgat
     3241 ccatccacac aggcccgccc tcagccgatg ggtcgactca taatctcccg gtgccaaggc
     3301 acggaccgtc acagttctcg cggcttccac gctgggaccc aaggctccca ccattgagac
     3361 cacggtcccc agcaaaacgc cctttgccac agccgcgaat gtcagcatgt ccatcgacag
     3421 aatcaacccg ccggacgtga tcggtacata caggtctgag atcgttcggc ttacgagcga
     3481 caccaaactc ctggcaagcc agattccacc aagtccaccc aatagcccgc ccaaaagacc
     3541 gagcaatcct gcttccataa gaaacaaacc ggccacccgc cactccgtca tgccgagtgc
     3601 gcgatagatc ccgatctcgc gtctccgttg cgtgacaaca aatgcaatcg tgttatagat
     3661 caagaacatc ccgacgagca gccccaccca gctcatgatc gccagattaa gttggaacgc
     3721 ccacaccatg ttttcaacct gtttcgtccg ttgcgtcgga cgttgtacga caaggtgcgg
     3781 ggggaggacc gtacgcagag aggcggcgat gtcgtcgagt ggtcgatcag gcgtcgtcac
     3841 caattcgatc cgatccagcc gtccaatcga ttggaataac aactgcgccg cagcaatatc
     3901 catgacggcc aaccgatccc aaagggacga gcgcgccgcc tcgttatgaa tcagccctac
     3961 aactctcaac cgtatgagac gcccccccac cgtcccctcg atgatactcc cgactcccag
     4021 attccagtcg gcagcaacct gtcgaccaag atacagagcg tccggagcca acagtgcttc
     4081 cagcgctgcc tcagtctctg cctccaatat ctgaaatcca cgcgtcctgg cctccgcaag
     4141 taggtccagc cccaatatct cgagcgtctg gccaggcgac tttccctgag cgaccacaat
     4201 ggcttcttca ataaccggag cggcgctcac gaccccttca accgcccgaa cggccgtaat
     4261 gaccgattca tcgactccga gctcatggcc tgcgatttcc aacgtggcag gaccggcaac
     4321 cgtcgtgacg gcctgctcga acgatcgcag gacttgcatg ttcgccgtcc cgatggccac
     4381 agacgccaga acacccagcg ccactccggc aatgctgagg agcgcacgaa acggccgctg
     4441 gagcgcatga gcgcgaatca gtgcgatcgg aaggaagagt ggtatagtca tctagggcct
     4501 agcaggatat agaaagaatc cgccggcgcc atggaccgag gcgtgtgaaa catggaagaa
     4561 cggcgcgatg ttaacaacct gagtcccgtc tctccacgct ctgctatact gtgggatacg
     4621 aatcttatag ggcgaattgt catggatgag ggcgtcgaga taaaccacat caatcgggcc
     4681 aataacagag tcctgcacaa atcggcagtt tggtttacag cgattcacac ctttgctaga
     4741 ctcgcgtgag ttgatcatga gaggttattg atgggcgcac gaactgctct gaaccgaaga
     4801 atccaaccgc tacaggaaga catcgacgcc gatcttctca cacctcggca gcgtgacatt
     4861 cttcgcctgg tgtcgatagg gcacacaaac cgcgagattt cggaggtgct ggcgatcagc
     4921 gtccgcacgg tcgaagtcca tcgattcaac ttgatgcgac ggctgaacgt gagaaacgtg
     4981 gcacagttac tccgacgagc gctgcagctg ggcctgctag ccaaaacatt cggagccaag
     5041 taagtctaca gctccttcag ctttccccgg cagtcctcta ccttcgtata gcctttcttg
     5101 ctgagctgcg tcgtcaactc cgtttccaac cggccaaaca cgtcgacccc ctcttcgacc
     5161 aagaccgtcc cgatctgcac cgccgaggca ccacagagca gatgttcaaa cgcatccatg
     5221 ccgttgacca cgccgccggt cccgataatc ggcatgcgcc cttcgaaaat cttccaaaaa
     5281 gccctcacgt tggccaacgc aaccggcttg ataatcgtac cacccagtcc accgaaccca
     5341 cccttgggct tgatgaggac gacctcccgc tccggatcga cgacgagccc gttgccgacc
     5401 gagtttatca agttgagaaa atcgacccca caacggagga gcacctctcc cattaccgca
     5461 tgatgggccg gatcgaaata gggaggcaac ttgaccccca tcgggaccgt aatcactttg
     5521 cggacacgct tcaatagacg ttcggaggcc tcggcgtcat atccgatctg aggtttgcca
     5581 ggtatgtttg gacaggagag attgacttcg atcaagtctg gcttcgccgc attgatcgtg
     5641 gtcgcgatcg tcgggaaatc gtcttcacaa agacctgcca cactggcgat aaccggcttg
     5701 ccgagcgact tcaattcagg aatgagcgcg gcgtatgcct tatacccgag attcggcaag
     5761 cccatggagt tgatggaccc accggaaaac ccgtagtacc gcggcgtcgg attgccgtcg
     5821 cgaggctcaa ccgtcatcga tttagtgacg atggccccgg cacgagatcg gccgaggacc
     5881 agcagttctt cacgtgtaac acagagggcg cccgaagcat tcatgaagca actgggaaat
     5941 cgcacgccgg caatggtggt cgaaagatcc atcacgcgac ttccgtgaca ggcgcgtgct
     6001 gtgttcttga caccagctgc ccatccttca tagcccagac ataatcggca gcctccgccg
     6061 ccgctgtgct gtgagtgacc agcagtacgg tttggcgaaa gcgctggacg agcgaccgca
     6121 ggaggacgat gatctcggcc ccatgctggg aatccaaatt gccggtcggc tcatcagcga
     6181 gaaggatctc gggctgatgc acgatcgctc gcgcaatcgc cacgcgctgc tgctccccgc
     6241 ccgaaagctc gctggggcga tggtgacgtc gcgaggtcat cgacacgacc tcaagcatct
     6301 cctcgacgcg ggcttggacc gtcccccctc gttccccgcg cagtaataga ggaaacgcga
     6361 cattctccgc agccgtgagc ccgggaatca gatggaatgc ttggaaaata attccgactg
     6421 tttcccgtct gatcctcgtc cagtcgttgc tcgtgaatcg actggtcgac cgccctccaa
     6481 gaaggatgtc acccgaggtg ggcacatcca atccggcaac taggttgagc aacgtactct
     6541 tcccgcagcc gctgggaccg ataaacgcac agaagtcccc cttcgccact tcgaccgtga
     6601 cccgatcgag cccggtgatc gtgtcgcccc cacgctcata aatcttcgat acctggtcga
     6661 agcttaccat cgtcatctgt ttctccattc gtaccctaag ccggcagaat cgccgcgcga
     6721 gactcgcgag aaaggccgga atcaagggtt gaacgtcgcg cttaggagca ccgacaggaa
     6781 tttattcatt atcctactgc cttcgtatat cataaccatt tatagagcaa caaccttgac
     6841 aggcgcggcc tcttctcata gaagaagcgt acacacattc gttggaacaa tttcgctgcg
     6901 atgttacgtg aaggcacaca ggaccgactc atcggatggt gccgtcaggc ccttcggttt
     6961 gtattgcccg tcgaatgctg tgcctgcggc cattcactcg gcaccgaccc ggtgcccttc
     7021 ttctgtaccg cgtgctggca caccatccgc ccactccagc agccggtgtg tgcccgctgc
     7081 gatcagccgt ttgcctcgcc ggcagccacg acctatactc cgaaccatca atgccaacag
     7141 tgtcaggaac agccgcctga gtttcagcgc gcctggaccc tcttcccgta tctttcgcca
     7201 ttacgagaag ccatatgctc gttcaagtac cggggcaaac atacactggc acggccactc
     7261 gcccgcctga tgatcagcgc cctgccaagc gggatcgaca tcgacgtgat tgtccctgtg
     7321 cccttacacc ctacccgctt acgcgcacgt gaatttaacc aatccttgct gctggccgat
     7381 cagctcagcc gtcatctggt ccgccctgtt tcgcccacac acctcgtcag gatcgccgcc
     7441 accgaccctc aaaccacatt aacaagacag gcacggttac gaaatctacg gaaggcattt
     7501 gaagtccgca aaccacagga cctcgtggag aaacgcatcc tgctggtgga tgatgtcttc
     7561 accactggga cgacgctgaa cgaatgcgcg aaaaccctac gcaaagccgg ttctggacct
     7621 gtctttgccc tgacgctcgc ccggactgtc gatacgagcc tgataccgga tcgtcacttt
     7681 gccgaacgaa ctctaccgac cctgacgacc attggaattt gatccatgcc gttgtttgaa
     7741 tatcgctgtc ttgactgtaa gaagcgcagc acggtccttg tcttgagcca ggccaatcaa
     7801 gctccggcag cctgcgccca ttgcacgagc gctcgggttg aacgaatcct gtctcgattt
     7861 gcctcgccga aatccgaagc ggctcggctt gaagccttgg cggatcccag cagcctggcc
     7921 gggctcgatg agaatgatcc acagagcatg gctcgattta tggaaacaat gggcgaggag
     7981 atgggcgaag atctcagtga tgatgtctca gcggcgatgg aatcagacgg gaccgacggc
     8041 atggaatcgg atggtactga caggctgtga caggcattgt catatatttt cacttgacag
     8101 attgtgagag tttccctacc ctggcaccag tatttaaagc ctcgtgaggc atatggatcc
     8161 ctatcgagcc aggtttttca atggccagag agcccaagag cgaactgatg acggcggagg
     8221 agacctgccg ctatctcaag atcacccaac gcacactcta tcgttaccta cagagtcgcc
     8281 agattcccgc cttcaagctt ggcaaagaat ggcgctttgt acgttcggat ctagaacagt
     8341 ggattcgcga tcgcaccaga gccacgctcg cctcataaag gacacgatac attgatgttc
     8401 gccggtgaat acctctgcaa ggtggatgag aagggacggt ttatcgttcc ctcaccgatc
     8461 cgtgagcagg ttgagtcgga gggacaggcc gtcgtcttcc tcaagggccc cgaggaatcc
     8521 acgctcattt actccatgaa agagtgggag aaagtcctcg aacggacaaa gaccacgctc
     8581 gatgaagacc agagtcgatt gttcatgcat ttcgtcgtat cagaagccgg cacctcagag
     8641 atcgacaaaa ccggacgcat cctgattccg agtcggctca gaaaaattat tccgttggat
     8701 gaagaccagg aaattgttct ggtaggctta taccatcgca tggagatctg gaacccgagc
     8761 acatggcgcc ggtatatttc ccagtctgaa gagcgatacg agcagaacat gtccaaaatc
     8821 ctcaatctcc tataagttca cgcgcatgcc tacgtttcgg cgcccgaccc gacttccttc
     8881 cgctccaagg gccgttcgca ccacctccta cagaaaccca ggcaagaagg tcccggtgtt
     8941 acgtattgcc ccgagaaggg ctccgagttc cagcataccg acctccccat taccgcaccc
     9001 accggctgag ggtcatgcgg tacacttaac cctccctgta ccccccagtg tgaaccatca
     9061 atatgctacg gtgaatggcc ggcgattgat ctcttcggct ggtcgtgcct ataaggccca
     9121 agtgggacaa ctcgtatggt tgaagctgtc ccaatccaca cagcgagctg ctctcctgac
     9181 acgactgcag tcggactggc tcgccctgtc catccgcttc tactttacct cggccctgcg
     9241 acgagatgtc gacggtggac tcaagattgc gcaggatgcc ctctgtgagg gtctcggcat
     9301 caatgacaac cgcatcgtcg aaacccacct ctacaaacat atcgataagc acaaccctcg
     9361 catcgaagtg catttgacac ctgcagccgg ttcctagggc tcccagccga tccacaagac
     9421 tcctcatctt ctcgacgatc catcataaag ggcgcaggct gcgccgcact tctccggcgc
     9481 ggtcgatgct gcggtggccc gccactggct gggagagc
//
LOCUS       DATIPF010000218         2426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_661363, whole genome shotgun sequence.
ACCESSION   DATIPF010000218 DATIPF010000000
VERSION     DATIPF010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661363"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VLL06_05575"
     CDS             complement(<1..505)
                     /locus_tag="VLL06_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HSS30468.1"
                     /translation="MPPNSSRIDISPSQETIPPVEPCTLVIFGGSGDLARRRLIPALY
                     NLLLDGLLPSHYAVIGLGRKLMTDEEFRATIRDGIVAHSRQALTEDKWKDFSPHLFYV
                     SGGNDDPNTYAKLKSRAEEVERNFQLPGNRIFYLSIPPSSFAPVCEGLEQAGLATKPG
                     AHMPYARI"
     gene            complement(518..1411)
                     /gene="gnd"
                     /locus_tag="VLL06_05580"
     CDS             complement(518..1411)
                     /gene="gnd"
                     /locus_tag="VLL06_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HSS30469.1"
                     /translation="MEIGFIGLGKMGMNMVTRLQRDRHRVVVYDRSADLIKQAEGVGG
                     VGASSLADLVGQLKAPRAVWIMVPSGAPTEETVQAVAALLSPGDTVIDGGNTRFHDDV
                     RRAADLKKKQLHYVDAGTSGGIWGLKIGYCLMVGGDEAPVKNLASVFTTLAPEQGWSH
                     VGGVGAGHYVKMVHNGIEYSMMQGYAEGFELMSKSDYRLDLGKIADLWMHGSVVRSWL
                     LELAAGALKDDPKLEQLKGYVQDSGEGRWMIADAIEKDVPVPTLATALFTRFRSRQSE
                     SFAEKMLAALRKAFGGHAVRH"
     gene            complement(1559..2344)
                     /locus_tag="VLL06_05585"
     CDS             complement(1559..2344)
                     /locus_tag="VLL06_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30470.1"
                     /translation="MDSPAAHPIPTSEELKAELLAMPEPPEQPDPIDEPGFEELVALA
                     VRHVRGRRGGDLVSVILVGSSVRRAITTHSDIDLIALVKGQADGHEIVRVADRLADIR
                     YRGHQEFEEDLPCSPRLPSLLRKARILFDHESIAARLIERANQRFRQGPPPVSINEQI
                     RMKAECFHWLGKARDLADRPATAQYLLSIFFDDVINAFFRLRGFWLTAPGDVPRFIAS
                     RDAELGDLAGRFLTAATLPERLNFGRDLADTLFKDVPNPARID"
BASE COUNT          502 a          722 c          679 g          523 t
ORIGIN      
        1 tgatgcgggc atagggcata tgagctcccg gcttcgtcgc caatcctgct tgctcaagcc
       61 cctcgcacac gggggcaaac gagctgggag ggatgctcaa atagaaaatt cggttgccgg
      121 gcaattgaaa attccgttca acctcttctg cccgagattt cagcttggca taggtgttcg
      181 ggtcatcgtt tcctcccgac acgtaaaaga ggtgtggcga gaagtctttc cacttgtcct
      241 ctgtcaatgc ctgccgggaa tgggccacaa tgccgtcacg tatcgtagct cggaactcct
      301 cgtcggtcat taacttgcgg cccagcccaa tcaccgcata atgcgatgga aggagtccat
      361 caagaagcaa gttgtagagg gcaggaatga gacgccgacg tgccagatca ccggaccccc
      421 cgaagatgac cagcgtacag ggctcgaccg gggggatcgt ttcctgtgac gggctgatat
      481 cgatacgtga actattcggt ggcataggct tcgggtgcta atggcggact gcatggccgc
      541 caaacgcttt tcgcaacgca gccagcatct tttccgcaaa ggattcgctt tgccgcgatc
      601 ggaatcgagt gaacaatgcc gtcgcgagtg tcgggaccgg gacatctttc tcgatcgcat
      661 cggcaatcat ccagcgtcct tcgccggaat cctgcacata gcctttcagt tgttccaact
      721 tcggatcgtc ttttaaggca cctgccgcca attccaagag ccaggagcgg acgacactcc
      781 cgtgcatcca gaggtctgcg atctttccga ggtcgagccg atagtcgctc ttcgacatga
      841 gctcgaaacc ttccgcatag ccctgcatca tgctgtactc gattccgtta tgcaccatct
      901 tcacataatg cccggcgccg acacccccga cgtgggacca gccctgttcc ggcgccagcg
      961 tcgtgaagac cgacgcaaga tttttcaccg gcgcctcgtc gccaccgacc atcaaacagt
     1021 atccgatctt cagcccccaa ataccgccgc tggtaccggc atcgacataa tgaagctgtt
     1081 tcttcttgag gtccgcggcc cgtcgaacat cgtcgtggaa tctggtgttg ccgccatcga
     1141 tcacggtgtc gcccgggctg agcagcgccg cgacggcttg aacggtttcc tctgtcggtg
     1201 cgccggatgg gaccatgatc cagaccgcgc gcggagcctt gagctgaccg acaagatcgg
     1261 ccaacgaaga ggcgccgaca ccaccaacac cttcggcctg tttgatcaaa tccgccgatc
     1321 gatcatagac taccacccgg tgtcggtcac gttgcaagcg ggtgaccatg ttcatcccca
     1381 tttttccgag tccaataaat ccgatttcca tactgactcc ttctgattcg gttatagctg
     1441 gacgagattg cgtggatcac acttcttgtg aagagcctct cacctattca gtcttgcata
     1501 agaggattgt ctgataagcc ccgtacgact gcggtctgat cattgacccg tcgtcctgtc
     1561 agtcgattct ggcaggattc ggtacgtcct taaagagcgt atcggcaagg tcgcggccaa
     1621 agttgagccg ttccggcaac gtggctgcag tcaggaagcg tccagccagg tctcccaact
     1681 ccgcatcgcg ggaggcgata aaccttggca cgtcaccggg tgcggtcagc cagaagcccc
     1741 gtaaccggaa gaacgcgttg atcacgtcat caaagaatat cgagagtagg tactgcgcgg
     1801 tcgccggcct gtctgccaga tcccgcgctt ttcccagcca atggaaacac tctgctttca
     1861 tccgaatctg ctcgttgatg ctcaccggtg gtggcccctg ccggaaccgt tggttggcgc
     1921 gttcgatgag cctcgcggcg atgctctcgt gatcgaagag gatgcgggcc ttccgcaaga
     1981 gagacggaag acgcggcgaa cagggaaggt cctcctcaaa ctcttgatgt ccgcgatagc
     2041 gaatgtcggc cagacgatcg gcgacccgga cgatctcgtg gccatcagcc tgtcctttga
     2101 caagggcaat aagatcgatg tcgctgtgag tcgtgatggc gcgtcgaacg ctggatccca
     2161 ccaggattac actgacgaga tcgcctccgc ggcggccacg gacatgacgc acagccaggg
     2221 cgacaagctc ttcgaacccc ggttcgtcga tggggtccgg ttgttcaggc ggttctggca
     2281 tcgccaggag ttccgccttc agttcttctg atgtcggaat gggatgagcg gccgggctat
     2341 ccatgattag gctccctgta tgagctgaat atgtttcggg agcgcgctca gccagtcctc
     2401 gaacggttgg tccgcatcaa cgacga
//
LOCUS       DATIPF010000219         3448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_661417, whole genome shotgun sequence.
ACCESSION   DATIPF010000219 DATIPF010000000
VERSION     DATIPF010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661417"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VLL06_05590"
     CDS             complement(<1..287)
                     /locus_tag="VLL06_05590"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HSS30471.1"
                     /translation="MSILFRYLLREYGKIFTMCFSGLMTIYLVIDFFEKVRRFLRYDA
                     DWLDVLTYFLLKVPAISFQIAPLAILMATLLTFGLLSRGHEITAMRSCGIS"
     gene            complement(292..1383)
                     /gene="lptF"
                     /locus_tag="VLL06_05595"
     CDS             complement(292..1383)
                     /gene="lptF"
                     /locus_tag="VLL06_05595"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HSS30472.1"
                     /translation="MFLRTLLDRYIFTELLFPFSISLGALCFVMLTRELLRLVELLVS
                     KGVGIVAVLQVFANLMPSFLVLTLPIAGIIASITAFGRLSFDKELVAMQTAGLSLLRL
                     ARPVLLFALLVFGLTVWLAQWGQPWSSINLKKVALNLLRDQLVLALERGTFNEPIPKM
                     MIYVPDGNAGQATTGIFVSDERNAEDPRIIVAEQFEVLMDPSTDQVALRLLHGVIHTK
                     RNHADQYEQASFASYDLKISLNQSGYSATEERPSYDTLIAQLTQSQWRDPSALRRLME
                     YYKDLAFPTASLIFCLLGIPAGIASKRSGRIGGFAVGVFVVIVYYMLNVACEFLVTTA
                     HLSPFLGAWIPNGIFFLTTVVWFYIMSRH"
     gene            1682..1900
                     /locus_tag="VLL06_05600"
     CDS             1682..1900
                     /locus_tag="VLL06_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HSS30473.1"
                     /translation="MKSKGTVKWFNDRKGFGFIRLESGDEVFVHYSALQGEGFKTLKE
                     GENVEFEVVQGAKGPQASNVLKMAVAQS"
     gene            2057..>3448
                     /locus_tag="VLL06_05605"
     CDS             2057..>3448
                     /locus_tag="VLL06_05605"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS30474.1"
                     /translation="MAIDRSSVLHSAQLFASRGQFDAAINEWKKLSAESPGDGSIFNS
                     IGELHLKRHATADAVTAFSQAANAFRAEGATLKAIATFKKILKLDPSRYDVFRHLGDL
                     NAERGLLSSAVQDYLTLGKHYLKVGKSKDALDIYKKIVSQDPSNLDAQQRVAELCLQE
                     NMQDEATKVYLQLGRERSAQQRYAEARDAYQAVLRIEPGNSEAAQFIEHFTKTGGGPS
                     QAANARAGTGGSPPKNTEPVNLLSEATRRIEARQFAGAEAILNQLLTKEPGNPEVCQL
                     LAKLHLQRGDLQVALGEYRYLAGAALRAQHYDQAESMMVEFLAIEPNSVPIIELYGEL
                     YEEKGDVANAVIRYGKAIELLLQHPEPGMPTLHEELFEKIKSLAPDDPILPKLSALMK
                     GESAIEIPSPAASQSSQDVSWTGTAPDDAWTVKSTIPLPHDIPESFLDPEPEPVPSEP
                     VPHAEPEPATASTG"
BASE COUNT          867 a          906 c          968 g          707 t
ORIGIN      
        1 aaactgatgc cgcaactgcg catggccgta atctcgtggc cgcgggagag cagaccaaac
       61 gtgagcaatg tggccatcag gatggccagc ggcgcaatct ggaaggaaat cgccggaacc
      121 ttgagaagaa aatacgtcaa cacatcaagc caatcggcat cgtagcgcag gaagcggcgc
      181 actttctcga aaaagtcgat gacgagatag atcgtcatca acccgctgaa gcacatagtg
      241 aaaattttcc cgtactcgcg aaggaggtag cggaagagaa tggacatggg gttagtggcg
      301 actcatgata taaaaccaca cgacggtggt cagaaagaaa atgccgttcg gtatccaagc
      361 ccctagaaac ggcgagaggt gtgccgtcgt gaccagaaat tcgcaggcga cattcagcat
      421 gtagtagacg atcacgacaa acacgccgac cgcaaatccg ccgattcggc ccgatcgttt
      481 tgaggcgatg ccggcgggga tgcctaacag gcaaaatatc agcgaagcgg tgggaaaggc
      541 cagatcttta taatactcca tcagccgtcg gagcgcggac ggatcgcgcc attgggattg
      601 tgtgagctgg gcgatgaggg tgtcgtacga tggacgttct tccgtggcgg agtagccgct
      661 ttggttgagt gagattttca gatcgtaact ggcgaaggat gcttgctcgt attgatcggc
      721 atgattccgt ttggtgtgaa tgactccgtg gagcaagcgt aacgccactt gatcggtgga
      781 aggatccatc aagacttcga actgctccgc cacgatgatg cggggatcct ctgcattccg
      841 ctcgtcggag acaaagatcc cggtggtggc ctgtccagca ttcccgtccg ggacatagat
      901 catcatcttg gggatgggct cgttaaatgt tccccgttcc agcgcgagta ccagctgatc
      961 tcgcaggaga ttcagcgcga cttttttgag atttatggaa ctccagggtt gcccccactg
     1021 tgccaaccac acggtcaaac cgaacacgag caaggcaaag aggagcacag ggcgcgccaa
     1081 gcgtaagaga ctcagacccg cagtctgcat cgccaccaat tccttgtcaa aagacagtcg
     1141 accaaatgcg gtaatcgagg cgatgattcc ggcgatcggg agggtcagta caaggaacga
     1201 tggcatgaga ttggcgaaga cttgcaggac cgccacgatg ccgacgcctt tggagaccaa
     1261 caattccacg aggcgcagga gttcccgcgt cagcattaca aaacacagcg ccccgaggct
     1321 gatactaaag gggaaaagaa gttcagtgaa gatgtagcga tcaagcagcg ttcgtagaaa
     1381 cattcggaga atcacgctcc tcgccgggag tatcactata ggtgagaggg caagacttgt
     1441 ctaccataag gccggcaatc atggggccaa agaatagctt gacaagattt gaaacgccgt
     1501 gatacatgct gccccgagtt gtcaccgaga tggtcttcgt gatcgatctt gtctctccaa
     1561 tggacccatc attccaaccg attcttcttc ctgacttgtt cgcgtcttca tgtgtgtcgt
     1621 cagttgggtt ccggagccga ccacgtaact cgtcgtggcc gttgaggaaa ggagtgtgtc
     1681 tgtgaaatca aaaggtacag tcaaatggtt caacgatcgc aagggatttg ggttcattcg
     1741 acttgagagc ggggacgagg tcttcgtaca ttactccgcg ctgcaaggag aagggttcaa
     1801 gaccttgaaa gagggagaaa acgtcgagtt cgaagtcgtc caaggcgcca aggggcctca
     1861 ggcttcgaac gtcttgaaga tggccgttgc ccagtcttga cgtgttcatt ctgttgggtt
     1921 gtgccatgag aaggcctgct ttttttcatt aagggcaggc cttcgtattt ctgctgagtc
     1981 tccctgcatc caaagcccca attcgttgac aatacctaga gacactgtta gcgtttgatc
     2041 gttggggagg tctgacttgg cgatcgaccg tagcagtgta ttgcatagtg cacagctttt
     2101 tgcgtcacgg ggccaattcg acgctgccat caatgaatgg aagaaactct ccgccgagtc
     2161 gccgggcgat gggagtatct tcaattcaat cggggagtta cacctcaaac gccacgccac
     2221 cgccgatgcc gtcaccgcct tttcgcaggc tgccaacgcc ttccgtgccg aaggggctac
     2281 cctcaaggcc attgcgacct tcaagaagat cttgaagctt gatccttcac gctacgatgt
     2341 gttccggcat ctgggagatc tcaatgcgga acgcggtctg ttgagtagcg cagtccaaga
     2401 ttatctcacc ctaggaaaac actacctcaa agtggggaaa agtaaagatg ccttggacat
     2461 ctataagaaa atcgtcagcc aggacccctc gaatctggat gcccagcagc gcgttgcgga
     2521 attgtgtctg caggaaaaca tgcaggacga ggccacaaaa gtctatctgc agttagggcg
     2581 ggagcggtcg gcccagcaac gctatgctga ggcaagggat gcctatcaag ccgtcttgcg
     2641 gatcgaacct ggcaatagtg aggctgctca gttcatcgaa cacttcacaa agaccggtgg
     2701 ggggcctagc caggccgcca atgcgagagc aggcaccgga ggaagtccac ccaagaatac
     2761 cgagccggtc aatctgctgt cggaagccac ccgccgcatt gaagcacgac aatttgccgg
     2821 ggcggaagcc attctcaatc aattgctcac caaagagccc ggaaatcccg aggtgtgtca
     2881 attgctcgcc aagttgcatc tgcaacgcgg agatcttcag gtggcccttg gagaatatcg
     2941 ctacctagcc ggggcggctt tgcgcgccca acattatgac caggccgaat cgatgatggt
     3001 cgaatttctg gctatcgaac caaactccgt accgatcatc gagctctacg gagaactcta
     3061 cgaagaaaag ggtgatgtgg ccaatgcggt gatccgctat gggaaggcca tcgagctcct
     3121 gctccaacat ccagaaccag gcatgcccac gctccacgaa gaactatttg agaagatcaa
     3181 atcgctcgct cctgacgatc ccattcttcc gaagctctct gcactgatga aaggggagag
     3241 cgccattgaa ataccttcac ccgctgcctc gcagagttcg caggacgtgt cgtggactgg
     3301 aacagcaccc gacgatgcct ggacagtgaa gtccacgatt cccttgccgc acgacatacc
     3361 tgaatcattc ctcgatccgg aacctgagcc ggttccatcc gaaccggtac cgcatgcaga
     3421 acccgaacca gctacggcga gtactgga
//
LOCUS       DATIPF010000220         4555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_925867, whole genome shotgun sequence.
ACCESSION   DATIPF010000220 DATIPF010000000
VERSION     DATIPF010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925867"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VLL06_05610"
     CDS             complement(<1..277)
                     /locus_tag="VLL06_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HSS30475.1"
                     /translation="MRDHTQLRAFELADEVVMLVYRTTVGFPKEEMFGLTSQIRRAAV
                     SVPSNIVEGCARDSETEYLRFLNIAFGSLRELHYQMNFSKRLGFLPTE"
     gene            complement(280..2022)
                     /gene="waaF"
                     /locus_tag="VLL06_05615"
     CDS             complement(280..2022)
                     /gene="waaF"
                     /locus_tag="VLL06_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HSS30476.1"
                     /translation="MDRAAIKRILVRGPNWLGDAVMCEPALRGLQRLFPEAQIALLVK
                     PAVADLFAGHPALTRVLTYDSHGTHAGLPGKWALAGQLRQQGFDLSVLFQNAFEAAFL
                     TFLAGVPRRYGYATDGRSLLLSHPVAPPDRRALVHQVRYYWDLLKPLGLTGEPPAPEL
                     VLLPEEEQAMAGRFAQGGVTASDIVIGVNPGSTYGEAKRWFPERFAEVTERLCRTMRE
                     SREQQVSVVILGAKGEERLGREIATRLSSRSLVLSGATTIRELMAAVKRCAMLLTNDT
                     GPMHIASAFRVPVVAIFGPTDWRTTSPIGDAHAIVRQPVDCAPCLLRECPIDHRCMTR
                     VSVEQVYEAGLSSLSSRGGLSGLSGQKDWTRPNQTDKTDLPHQIDQRNILDGVTVFLD
                     RDGTLNYDPGYLKVAADLKLLAGVGPALARLKRAGARLVVVTNQSGVGRGVVTLKDLE
                     AIHARLQGLLEQEGAALDAIYFCPHHPNDGCRCRKPNVGMVERAVSELQLDLRRAYLI
                     GDHARDIQLANRVGAKAILLTPASVDAQALDRLKVEQALPDAVAQSMAEAADWILKDA
                     KDTFQVDRLKAFRG"
     gene            complement(2015..3058)
                     /gene="lpxK"
                     /locus_tag="VLL06_05620"
     CDS             complement(2015..3058)
                     /gene="lpxK"
                     /locus_tag="VLL06_05620"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HSS30477.1"
                     /translation="MWAAIPYGVVAGLRALLYDWGWFAQRKLPVSVLSVGNLTLGGTG
                     KTPVVIVLVDWLLAQGKRVAILSRGYRRTSTDPFLLVSDGERLLVNPKEAGDEPFLMA
                     QRCPKAIVAVGADRYELGDWVLNRFPVDCLVLDDGYQHLGLYRDVNLLLVDATDAEGL
                     AALVPSGRLREPLQAAARATAIVVTRADVPAQVTEVCHRLRATLGTMPDPIQVVFRPE
                     SLVSVVTGTSQTLSWSKGKTALLCSGVGHAGSFRSLVERMGMKVLDEVAYADHHDYTS
                     HDVERLRARAAELQAELIVTTEKDACKLASLLQHTDSWWAVRLTTYVSAGEERLKQLV
                     LSRPNRIPAEACG"
     gene            complement(3192..>4555)
                     /locus_tag="VLL06_05625"
     CDS             complement(3192..>4555)
                     /locus_tag="VLL06_05625"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HSS30478.1"
                     /translation="QRCRRGLLYRLGWKAGLFGSSGSSRSSGGFDQEKLNKPDQPNKP
                     NQPVIWIHAVSLGEVMAVTPLVIELHRRHPECRLVVSTVTETGREAVEQRLVGVADHC
                     YAPLDFPCVVSRFIEQLQPRLYLFVETELWPNLLWHLRRRGVPTALVNGRLSTRSFAR
                     QQWAPVRSFYRTMLQTLSLCLMQSDRDVDRIIALGAEASRVRRTGNIKFDQPIPAVTG
                     GGAARAYLGLQETEQLFVAGSTHSGEEEMLVECYRALVAQCPSAVLLLAPRHIERAES
                     VEAMIRARGIAVQRRSTIGQGAAQGPTGPRVVVLDSRGELAAIYREAVVAFVGGTLVP
                     IGGHNLLEPAQWAKPVLFGPYTDHCAEIADLLIHAGGGHRVLEAEDLTRQVMALFSDN
                     KERERMGRSARQVVEQNQGALQQTLEAIDRLLTTHQGSSGSPYADRSLPLMAEKSGTY
                     SGR"
BASE COUNT          988 a         1467 c         1185 g          915 t
ORIGIN      
        1 tctcggtggg caagaagccc aggcgcttcg agaaattcat ttgatagtgt agttccctca
       61 acgacccgaa ggctatattg agaaatctga gatactctgt ctcactgtca cgagcgcaac
      121 cttccacgat attagaagga actgaaactg ctgcccttcg tatttgagaa gtcagtccaa
      181 acatctcttc ctttgggaac cctacggtcg ttcgatacac caacatgacg acttcatcag
      241 ccagttcaaa tgcccgaagc tgtgtgtgat cccgcatgct taacccctaa acgccttcag
      301 cctatctacc tgaaatgtgt ctttggcgtc cttcagaatc cagtcggcgg cttcagccat
      361 agactgtgcc acggcatccg gcagagcctg ttcaacctta agccgatcca atgcctgcgc
      421 gtctaccgat gctggtgtaa ggagaatcgc cttggcgccc accctattcg ccagttgtat
      481 gtcgcgcgcg tgatcgccga tcaaataggc acgtcggaga tccagttgca gctccgacac
      541 ggcccgttcc accatgccga cgttcggttt gcgacagcgg cagccatcgt tcggatggtg
      601 cgggcagaaa tagatcgcat ccagtgccgc accctcctgt tccaacagtc cctgcaatct
      661 ggcatggatc gcttccaaat ccttgagtgt cacaactcca cgaccgacac cggattggtt
      721 ggtcacgact accaatcgcg ccccggcccg ttttaatcga gccagcgctg gcccgacccc
      781 tgccagcaac ttcagatcgg cggcaacttt caaatagccc ggatcgtagt tcaacgttcc
      841 atcccgatcc aggaacacgg tgactccgtc gagaatgttt ctctggtcta tctggtgggg
      901 gaggtctgtc ttgtctgtct ggttcggacg agtccaatct ttctggccag atagaccaga
      961 cagacctccc cgactagata gactagatag acctgcttca tagacctgct cgacagacac
     1021 ccttgtcatg catcgatggt cgatgggaca ttcccgcagc aggcagggag cgcaatcgac
     1081 cggctgccgc acaatggcat gggcatctcc aatgggcgat gtggttcgcc aatctgtcgg
     1141 cccgaagatg gccacgaccg gtaccctgaa ggcggaggcg atatgcatgg gaccggtatc
     1201 gttcgtgagg agcatggcgc atctttttac cgccgccatc agttcccgga tcgtcgtggc
     1261 ccccgacaac actagagatc gggacgacag acgcgtggcg atctcacggc ccaaacgctc
     1321 ttcccccttg gcccccaaaa tgaccacgct gacttgctgc tcacgcgatt cgcgcatcgt
     1381 gcggcacagt cgttctgtga cttctgcaaa ccgttccgga aaccagcgtt tcgcttcgcc
     1441 ataggtcgag ccggggttga ccccaatgac gatgtccgat gcggtcactc caccctgggc
     1501 aaatcgccct gccatcgcct gttcctcttc cggcaatagg acgagttccg gtgcaggggg
     1561 ctcacccgtg agacccaggg gtttcagcag atcccaataa taacggactt gatggacgag
     1621 cgcgcggcgg tccggcggag cgaccggatg ggacaatagc aaacttcgcc cgtctgtcgc
     1681 atacccgtag cgccggggca caccagccag gaacgtgagg aacgccgcct cgaacgcatt
     1741 ctgaaataag accgagagat cgaaaccctg ctgccgcaat tggccggcca aggcccactt
     1801 acctgggagc cccgcatgcg ttccgtggct gtcgtaggtc agcacacgtg tcaatgccgg
     1861 atggcccgcg aataaatcgg ccacagccgg tttgaccaat aacgcgatct gcgcttcggg
     1921 gaacagccgc tgcaatccac ggagcgccgg ttcgcacatc accgcatcgc caagccagtt
     1981 cggtccacgc acaagaattc gtttgatggc tgctctatcc acaggcctcc gccggaatgc
     2041 ggttcggtcg actcaacacg agctgcttga ggcgttcttc ccctgcgctc acatacgtgg
     2101 tcagtctgac tgcccaccag ctatccgtat gttggagcag cgaagccaat ttgcaggcat
     2161 ctttctccgt tgtcacgatc agttcagcct ggagttcagc cgccctggct ctcagtcgct
     2221 cgacgtcatg gctcgtatag tcatgatgat cggcataggc cacttcatca agaaccttca
     2281 tccccattcg ttcaacaagg gagcgaaatg atccggcatg ccccacgcca ctacagagca
     2341 atgcggtttt tcctttggac caggagagtg tttgcgacgt tcccgtcacc acggacacga
     2401 gactctccgg acgaaagacc acctggatcg gatcgggcat cgttccgagc gtcgctcgca
     2461 gtctgtgaca gacctctgtc acttgcgccg gcacgtccgc cctcgtcacc acaatcgccg
     2521 ttgcgcgcgc cgccgcctgt agcggttccc ggagccgacc ggacgggacc agggccgcaa
     2581 gcccctccgc atccgtggca tcgaccagca agagattcac gtcacgatag agccccagat
     2641 gctgataccc gtcgtcgagt accagacaat cgaccggaaa tcgattcaag acccagtcac
     2701 caagctcata ccgatcagca ccaaccgcga cgatcgcctt gggacaacgc tgcgccatca
     2761 agaatggctc atccccagcc tcttttgggt tgaccagcag acgctcacca tccgacacca
     2821 agagaaatgg atctgtactg gtccgccgat aaccccgact gagaatcgcc acccgtttcc
     2881 cctgtgccag aagccaatcg acaagcacga tcacaacggg cgttttcccc gtcccaccca
     2941 acgtgagatt tccgacactc agcacagaga ccggcaattt ccgctgagca aaccagcccc
     3001 agtcgtacag caacgcgcgc agccccgcaa ccaccccata agggattgcg gcccacatca
     3061 gccaccacct gaccttttcc ccaggactga gcattcttcg tattactcca ggggttcagg
     3121 ctgaaggtca taaggctgaa ggctaaaagc cttcggtctt cagtctatcg acctgagttc
     3181 tagccttcag cctatctacc tgagtaggta ccacttttct cagccataag aggaagcgac
     3241 cggtctgcat aaggagaccc gctgctgcct tgatgcgtcg tcagcagtct gtcgatggcc
     3301 tctagcgtct gttgcaaggc tccctgattc tgttcgacca cctgacgagc cgaccggccc
     3361 atccgctccc gttccttgtt atcagaaaac agcgccatca cctgtcgagt gaggtcctcc
     3421 gcctcgagaa cccgatggcc acctcccgcg tgaatcaaga ggtctgcaat ctccgcacaa
     3481 tggtcggtat agggaccgaa caataccggc tttgcccact gggccggctc cagtaaattg
     3541 tgtccaccaa ttggaacgag tgtacctccg acgaacgcca ccaccgcttc ccggtagatc
     3601 gcagccaatt cgccccgcga atcgagtacc acaacgcgag gaccggtagg cccctgcgca
     3661 gcgccctgac cgatggtact gcgtcgctgc acggcgatcc cacgcgcccg aatcatcgcc
     3721 tcgaccgatt ccgctcgttc aatgtgtcga ggagccagca ataagactgc ggagggacac
     3781 tgcgccacga gggctcgata gcattccacg agcatctctt cttcaccaga atgggtactg
     3841 cctgccacaa acagctgttc agtctcctga agcccaagat acgcccttgc cgctccccct
     3901 ccagtcacgg ctggtatcgg ctggtcgaac ttaatattcc ctgttcgcct gactcgcgag
     3961 gcctctgctc ccagcgcaat gatacgatcg acatcccggt ccgactgcat caggcagagg
     4021 ctgagcgtct gcaacatcgt ccggtagaat gatcgcacgg gcgcccactg ctgccgcgca
     4081 aaagacctgg tcgagagccg accgttcaca agagccgtcg gcactccacg ccggcgcaga
     4141 tgccataaca gattgggcca gagctccgtc tcgacgaaca gatagaggcg gggttgcagt
     4201 tgttcgatga agcgggacac gacacaggga aagtcgagcg gtgcataaca gtgatcggcc
     4261 acgccgacca gccgttgctc caccgcttcc cgtccggtct cggtcaccgt cgagaccacc
     4321 agacggcatt cgggatgacg gcgatgcagc tcgatcacga gcggtgtcac cgccatcact
     4381 tccccgagag acacggcgtg aatccaaatg actggttgat ttggtttgtt tggttggtct
     4441 ggtttattga gtttctcttg gtcgaaacca ccagatgaac gagaagaacc agatgaacca
     4501 aacaaacctg ctttccatcc cagccggtac agcagtccgc ggcggcatcg ttgtt
//
LOCUS       DATIPF010000221         4844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1193006, whole genome shotgun sequence.
ACCESSION   DATIPF010000221 DATIPF010000000
VERSION     DATIPF010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193006"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..1793)
                     /locus_tag="VLL06_05630"
     CDS             complement(276..1793)
                     /locus_tag="VLL06_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HSS30479.1"
                     /translation="MRQIVRRTIGCAIAVVVLSTPLIWGSQSVSSSHASSPATSVAAP
                     VALTRSAAPSNGFAEVAKAVTPAVVNISTVTVERVLDSRGVPDELRDRMEEFFGGPGG
                     PFRPREFRGPQRPGPGEPREHRSSGQGSGVIVSPDGYILTNNHVIDGARTVTITLPDK
                     REFRGKIVGADPKTDLAVVKIDGQNLPTVSWGDATQLQVGEYVLAVGNPFGLNSTVTL
                     GIVSALGRGHMGITQYEDFIQTDAAINPGNSGGALVNTKGELVGINTAIFSQTGGYQG
                     VGFAVPTSMSKPIYESLVKHGKVVRGFLGISIQDLTQDLAKSFDIKDAKGALVSDVKD
                     EGPADHAGLKQGDIIRTYQDSPIDDAAALQRQVTKTAVGTKVTIRVIRDGHEKDLTVT
                     IGEQPESTKIAKAETGETEYAFAGVAVQDLDRDTAKELGVKGKGQGVVVTAVEPDSGA
                     EKAGVMRGDVIREINRQPVKSLKDFERVSSGLKKGENVLILIDRRGSAVFLSAKV"
     gene            complement(1981..3429)
                     /locus_tag="VLL06_05635"
     CDS             complement(1981..3429)
                     /locus_tag="VLL06_05635"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HSS30480.1"
                     /translation="MPLRVRLTLWYGTALALILIIFSTILYVITARSLRDAVDQSLEE
                     TAAAAVRSLEERGFLPLIDEDELMSQFPELARIDKFFQIFSPSGTITIRSPNVKQHEM
                     PLSRQALDVAISGRMLFEFANYPKEPPLRLVSVPIIYRGNLLYIIQVGTSMDSVEQTL
                     TRLMLVLLVSMPIAVAVSLAGGWFMAGRALRPVDAITVAAQRIAGGDLTQRLKVPSAA
                     DEIGRLTKTFNDMIDRLETSFRQIRQFSSDASHELRTPLTVMKGETELALRRPRETED
                     YKVVLESNLEEIDRMTRIVDELLFLSRADMGEVKMEHVPVPLDMLVEDLHRQASLLGQ
                     EQDIEVVLRATAPAVVLGDELRLRELFLNLLDNAVKYSRPGGIVDIALTIEQTHARVT
                     VTDHGIGIAKEDQARIFDRFYRTDDARAHTKKGTGLGLAICAWIVESHRGHLEVQSKI
                     GEGSSFTVILPLAPASTHRMLKEAASVTDRSA"
     gene            complement(3429..4100)
                     /locus_tag="VLL06_05640"
     CDS             complement(3429..4100)
                     /locus_tag="VLL06_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSS30481.1"
                     /translation="MRILVIEDETKVGCFIKRALDEESYAVDLCEDGAKGLQMALDTN
                     YDLLIVDLMLPSLSGLDILKGVRQARIQTPILILTAQSQVDQRVKGLDAGADDYLTKP
                     FAIDELLARIRALLRRGSTESPGVLQIDDLALNPATREVTRDGQRIDLTLKEYALLEY
                     LMRHTGRVLTRPMISEHVWNQDFDTFTNVIDVYVNYLRNKIDRGRSKRLIHTIRGSGY
                     MLKAD"
     gene            complement(4189..>4844)
                     /locus_tag="VLL06_05645"
     CDS             complement(4189..>4844)
                     /locus_tag="VLL06_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HSS30482.1"
                     /translation="HYQMGGVQTNQWTETGITGLYAAGECACVSVHGANRLGGNSLLE
                     TIVFGRRAGIRAGEYARTVAPQALTTDQLTIEERRVERLLAEKGTVRAWQIREELGQL
                     MSLNLGLVRTHESMSAAISALTALIHRAASVTVQDKGHVFNTDLVQAFELQSLLDVAE
                     TIVVTALARKESRGAHYRSDFPVRDDQQWLRHSLIRRTSEGTALAYEPVTVTRFPPK"
BASE COUNT         1007 a         1415 c         1344 g         1078 t
ORIGIN      
        1 ccagtttcgt tctcgcatcg ttcagaccct caacgttgag gaggagtttc ggaaggactg
       61 gaggggcttt tccgttcgcc aagactcatt agaatggcga acggtctcac gaagtgcggt
      121 aggtacctcc tcaggccctc actcgttgcg gccttgccgg acgaactttt tgagcatccg
      181 gcttggaaga tttggaagaa atttagaaag ataaagatga agacagacac gatggggcag
      241 catccccaac tgccccatcg tcgtgattgt aatcgtcaga cttttgcgct gaggaacacc
      301 gcgctgcctc gccggtcgat caggatcaag acattctcgc ccttcttcag cccggacgac
      361 accctttcga aatccttcag cgacttcacc ggttgccggt tgatttcccg gatcacgtca
      421 ccccgcatca ccccggcctt ctctgcgccg ctgtccggtt caaccgccgt gaccactacg
      481 ccctgtccct tccccttgac cccgagttcc ttcgcagtgt ctcgatcgag gtcctgaaca
      541 gccaccccgg cgaaggcata ctccgtctca ccggtttctg ccttggcgat ctttgtgctc
      601 tccggttgtt cgccgatcgt caccgtgagg tctttctcgt ggccgtcacg gatcacccgg
      661 atggtcacct tcgtgcccac cgccgtcttg gtcacctgcc gctggagtgc cgcagcgtcg
      721 tcgatgggtg agtcttgata cgtcctgatg atatctccct gcttgaggcc tgcgtggtct
      781 gcagggcctt catcctttac gtcactgacg agggcacctt tcgcatcttt gatgtcgaac
      841 gatttggcca gatcttgggt cagatcttga atcgagatgc ctagaaaccc acgcacgacc
      901 ttcccatgct tgaccaggct ttcataaatc ggcttgctca tgctggtggg aaccgcaaaa
      961 ccgacgccct gatagccacc ggtttgagag aagatcgctg tattgatccc caccaattcg
     1021 cctttcgtat tgaccaaggc gccaccggaa ttcccaggat tgatcgccgc gtcggtttga
     1081 atgaagtcct cgtattgggt gatgcccata tggcctcgac ccagggcact cacgatgccg
     1141 agggtgacag tggaattcag gccaaaggga tttccgaccg ccagcacata ttcccccacc
     1201 tgcaattgtg tggcatcacc ccaagagacc gtgggaaggt tttgtccatc gattttcacg
     1261 acggcgagat ctgttttcgg atctgccccg acgatcttgc ctctgaattc ccgcttatcc
     1321 ggcagcgtga tggtgacggt ccgagctccg tcaatcacgt ggttgttcgt taggatgtac
     1381 ccgtctggtg agacgatgac accggacccc tgcccgctgc tccgatgctc tcgtggctca
     1441 ccagggccag gtctttgcgg tccacgaaat tcacgcggcc ggaacggtcc ccccggtcct
     1501 ccaaaaaact cttccattcg atcgcgcagt tcatcaggta cgcccctgct atccaacacc
     1561 ctctcgacgg tcacggtgct aatgttgact accgccggtg tcaccgcttt cgccacttct
     1621 gcaaatccat tcgatggcgc ggcactcctc gtgagggcaa ccggcgccgc taccgaagta
     1681 gctggactcg atgcatggga agaggatacc gattggctgc cccaaatcag aggggtgctc
     1741 agcaccacca ctgcaatggc gcatccaata gtccgacgaa cgatctgtct catgatttcc
     1801 tccttgcggt tgctcatacg agatgaagat atgtcacgac gtccaatttc gtgaataccg
     1861 taccagtcgg caatgagaag agtattagga aggaattaga aatcgttaag gcgcactatt
     1921 catgaagatt cttcgcgaaa acctccagcg gcgaagccga gactgcacgt tgcgtccgct
     1981 tcacgcgcta cgatccgtga cgctggcggc ctctttcaac atgcggtggg tcgatgcggg
     2041 agccagaggc agtatgaccg taaaactcga accttcgccg atcttgctct ggacttcgag
     2101 gtgcccgcga tgggattcaa caatccaggc acagatggcc aacccaaggc ccgtgccttt
     2161 cttcgtatgg gcccgcgcgt catcggtgcg atagaaccgg tcgaagatcc gagcctgatc
     2221 ctctttcgcg atcccgatgc cgtgatcggt caccgtcacc cgtgcgtgag tctgttcgat
     2281 cgtcagcgcg atgtcgacta tgccgccggg gcgagagtat ttgaccgcat tatcgagaag
     2341 attcagaaag agttcgcgca agcggagttc atctcccaat accactgccg gcgcggtggc
     2401 tcgcagtacg acctcgatgt cctgttcctg tcccaacagc gaggcctgac ggtgcaggtc
     2461 ctcgacaagc atgtccagtg ggaccggcac gtgttccatc ttcacctcgc ccatgtcagc
     2521 cctggagaga aagagcaact catccacgat acgcgtcatc cgatcgattt cttcgagatt
     2581 actctccaag acaactttgt agtcttcggt ttcgcgagga cgccgaagcg cgagttcggt
     2641 ttcacctttc atcactgtga gtggcgtgcg cagttcgtgc gatgcatcgc tgctgaattg
     2701 acggatctgc cgaaaggatg tttcgagtcg gtcgatcatg tcattaaacg tcttggtcag
     2761 acggcctatc tcgtcggccg cagacgggac ctttagtcgt tgtgtgagat cccctccggc
     2821 aatgcgttgg gcggccaccg taatcgcatc gactggacgc aaggctcggc ctgccatgaa
     2881 ccagccgccg gcgagcgaca cagccacggc aatgggcata ctgacgagga gcactaacat
     2941 gagacgggtc agggtctgtt caacggagtc catcgaggta ccgacctgga tgatatagag
     3001 caggttgccc cggtagatga tgggaaccga aacaaggcgc aacggcggtt ccttgggata
     3061 gttagcgaat tcgaacagca tgcggcctga gatggctacg tccaaggcct gacgactcaa
     3121 cggcatctca tgttgtttga cgttcggcga acgaatggta atggtgccgg agggactgaa
     3181 gatctggaag aacttatcga tgcgcgccag ttcggggaac tgagacatga gttcgtcttc
     3241 gtcgatcagc ggaaggaacc cgcgctcctc cagcgaccga acggccgctg cggcggtttc
     3301 ttccagcgat tgatcgaccg catcacggag gcttcgcgcc gtgatcacat acaggatggt
     3361 tgaaaagatg atgaggatga gcgcgagggc tgtgccgtac caaagcgtga gccggacacg
     3421 aagcggcatc agtcggcctt cagcatatat ccgcttccgc gaatcgtgtg aatcagcctc
     3481 tttgagcgtc cacgatcgat cttgttgcgg aggtaattca cgtagacatc aatgacattg
     3541 gtgaacgtgt caaagtcctg gttccacaca tgttctgaaa tcatgggacg ggtcaacaca
     3601 cggcccgtat ggcgcatcag atattcgagt agcgcatatt ccttcaacgt gaggtcgatc
     3661 cgctgaccgt ctctggtcac ttcgcgcgtg gcgggattca atgccagatc gtcgatctgg
     3721 agcacgcctg gactctcggt cgagccgcgg cgaagcaacg cacgaattcg ggcgagtaat
     3781 tcgtcgatag cgaagggctt ggtgaggtaa tcgtccgctc cggcatcaag gcctttgacg
     3841 cgctggtcta cttgagactg ggcggtcaga atcaggatcg gcgtttgaat cctggcctgc
     3901 cggacaccct tgagaatgtc cagtccagac aatgacggca acatcaaatc gacgatcaat
     3961 aagtcgtaat tcgtgtcgag agccatctgc aggcccttcg caccgtcttc acagaggtcg
     4021 acggcgtagc tctcctcatc aagcgcacgc ttgatgaaac agccgacctt ggtttcgtct
     4081 tcaatgacta gaatacgcat ggcgatcgat ggaggattat accaaacctg tgcctacaat
     4141 ttgtctagcc gcatccaggg aacgcccgca gcctcccagg gggagcgatt atttcggcgg
     4201 aaagcgtgtg acggtcacag gctcataggc caacgccgtt ccctcggaag tccgccggat
     4261 gagactgtgt ctcaaccact gctgatcgtc gcggaccggg aagtccgacc ggtaatgggc
     4321 cccacgactt tccttcctgg cgagggcggt gacgacgatc gtctccgcca catcgagaag
     4381 ggactggagt tcgaaggctt gcacgagatc cgtattaaac acatggcctt tgtcctgcac
     4441 cgtcacagag gcggcacgat ggatcagtgc agtgagtgcc gatattgcag cagacatgga
     4501 ctcgtgagtc cgaaccaagc caagattgag actcatcaac tggccgagtt cttcccgaat
     4561 ctgccaggct cggactgtgc ccttttctgc taatagacgc tcgacgcgac gctcctcgat
     4621 ggtgagctga tctgttgtga gtgcctgtgg cgcgacggtt cgtgcatact caccggctcg
     4681 aattccggcc cgccgcccga acacaatcgt ttcaaggagc gagttccctc cgagtcgatt
     4741 ggcgccgtgc acactgacgc aggcacattc accggccgca tagaggccgg taatgcccgt
     4801 ttccgtccat tgattggtct ggacgccgcc catctgatag tgcg
//
LOCUS       DATIPF010000222         4734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1546315, whole genome shotgun sequence.
ACCESSION   DATIPF010000222 DATIPF010000000
VERSION     DATIPF010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546315"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..890
                     /gene="tpx"
                     /locus_tag="VLL06_05650"
     CDS             234..890
                     /gene="tpx"
                     /locus_tag="VLL06_05650"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HSS30483.1"
                     /translation="MRAAHQMIVGAALCVALGLTGCGTTGGSGFNYKNIPVADGSTMA
                     GEGNRVLFKGKPLDLSGTSIKVGDPLREVNLTQTDLSQISITDTKGKGKVRIISVVPS
                     LDTPVCEQQTHYLSEKNKGLDKMVELVTVSLDTPFAQKRFAGDARITNVTFLSDHRGA
                     EFGMTYGLYLKGPHVLARTIMVIDAQNTVRYLQVTPELTQLPDLDEAFAVAKSLVTGS
                     "
     gene            1030..2433
                     /gene="sthA"
                     /locus_tag="VLL06_05655"
     CDS             1030..2433
                     /gene="sthA"
                     /locus_tag="VLL06_05655"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HSS30484.1"
                     /translation="MGQYDLLVIGTGPAGQKAAVQAAKLGKKVGIIERKEVVGGVCTN
                     TGTIPSKSLREAVLYLAGFRQRTIYGATYRLKKTITIEDLAFRANHIIKNEVEIVQDQ
                     MARNGIDLIYGEARFLDPHWLLVQQAGESIEHHAHVIVIAVGTEPARPADIPFDEDTI
                     IDTDGFLRLKHLPSSMVIVGGGVIGTEYASILAMMGVPVILIDKRPRLLEFIDAQIID
                     ALQQHMTDIGVTLYHEEGVVTIRKEPDGQITVSLTHRPPISTTTLMYAIGRIGATKPL
                     NLEAIGITPDPRGRLTVNEHFQTSVPHIYAVGDVIGFPALASTSMQQGRHASCHAFNH
                     PDSTDTDLLPYGIYAIPEISTVGRNEEELAKAGIPYGVGIAHYREIARGQLIGDETGM
                     VKLLFHRHTRALMGVHAIGEGATELIHIGQAVMAFQGKIDYFIDTIFNYPTLAECYRV
                     AALDGINRLPRPWAPHE"
     gene            2513..3187
                     /gene="tenA"
                     /locus_tag="VLL06_05660"
     CDS             2513..3187
                     /gene="tenA"
                     /locus_tag="VLL06_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HSS30485.1"
                     /translation="MHMSFSDHLRKVAQPIWDAQLTHPFVVALGKGTLPERKFKYYIL
                     QDARFLGDLARVFSAAAMRAPDSESALRLTRLAEETIVVERSLHEGYGSRWKMSAKEM
                     TAVPMAPTNYAYTRHMLTVAHTGSAAEVTVVALPCAWIYCVVGQHLLKQGPPKHNHPY
                     RDWLMLYASPEFAEVQRWMRSKVDQWAKTAGNAEKRRMEESFVASSRYEWMFWEMAWN
                     EEKWPV"
     gene            3871..>4734
                     /locus_tag="VLL06_05665"
     CDS             3871..>4734
                     /locus_tag="VLL06_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30486.1"
                     /translation="MNRLLIMVLIGVSALWTLPAYAGLSYEESLKQLAEGVIADAVKA
                     KRGRLALVDFTDAKGTVTPIGRFLAEELGTQILVAGELKVADRTIVGSTLKKFHVTQF
                     EPAQAHAVKQAAKAVDADVFATGSYLESPDGLRVTVKLLSPSTVQSLGATRGTLPKTG
                     PLAELIKETNKPPVVKMDLSAKPAPPPGLGFHSNEQYQLVVTALHKRNTQLSADLTIE
                     NKSSRNIKVLCLLQDTVLEDDQGTQWKLAVENNREGLCMRGIELSPRAKVMTALTFSA
                     PADAHESQLILH"
BASE COUNT         1100 a         1387 c         1342 g          905 t
ORIGIN      
        1 gtgtccgaca ttgaccatga cgggtgatgt gcgaaaccgg cgagaaagac gcgacgtgga
       61 cagattgagg tcctctcttg tttgtcctgc ttcactcggc ctgcctgtct cgcgcgggga
      121 ttcagcagat cagcaaaggt gggctgtacc gtcatcgctt cttgctcacg acacgagcac
      181 aaccagcatg ttcttgatag aatagacacc gttacttcac caaggagggt cgtatgaggg
      241 cagcacatca gatgatcgtt ggagccgcac tgtgcgtggc gcttgggctc accggatgcg
      301 gaacgaccgg cggaagcggt tttaactaca agaacatccc tgtcgctgac ggcagcacca
      361 tggcagggga aggcaatagg gtcctcttca aaggaaaacc cctagacctt tcaggaacca
      421 gcattaaagt cggcgatccc ctccgggagg tgaacctaac tcagaccgac ctgtcgcaga
      481 tctccattac cgacacaaag gggaagggca aggtccggat catcagtgtc gttccgtcac
      541 tcgacacgcc ggtatgtgaa caacagaccc actatctgag cgagaagaac aaaggcctcg
      601 ataagatggt cgaactggtc accgtgagcc ttgacacccc ttttgcacaa aaacgtttcg
      661 caggagatgc ccgtatcacc aacgtgacct tcctctcgga ccatcgaggt gctgaattcg
      721 gaatgaccta tggactgtac ctcaagggcc cccacgttct ggcacgtacc atcatggtga
      781 tcgacgccca gaacactgtg cggtatctgc aggtcactcc cgaactgacc cagctgccgg
      841 acctggatga ggcttttgcc gtggccaagt cattggtcac cggaagctag gccgaggaag
      901 gacagggtcg aaattgagac cgacgccata ctgtctgagg ttccgcgttc gcggtgtgga
      961 catacgaacg cggagcctca aacggtactc tcaatatccg aacgtcagcc tgtatcggca
     1021 ggacgcctga tgggccaata cgatctcctc gtcataggga ccgggccagc cggccagaag
     1081 gccgcggtcc aggccgccaa gctcggcaaa aaagtcggca tcatcgagcg caaagaagtc
     1141 gtcggcggcg tctgtaccaa caccggcacc atccccagca aatctctccg cgaagcggtc
     1201 ctgtatcttg cggggttccg tcaacgcacc atctacggag ccacctatcg cctgaaaaag
     1261 accatcacga tcgaagatct cgcctttcgc gccaaccaca tcatcaaaaa cgaagttgag
     1321 atcgtgcagg accagatggc tcgcaatggc atcgatctga tctatggaga ggcccggttc
     1381 cttgatcccc actggctcct cgttcaacag gccggtgaat cgattgagca tcatgcgcat
     1441 gtcatcgtca tcgccgtcgg gaccgaacca gctcggccag ccgacattcc ctttgacgaa
     1501 gacaccatca tcgataccga cggttttctc aggctcaaac atcttccatc atcgatggtc
     1561 atcgtcggcg gcggcgtgat cggcacagag tacgcatcga tcctcgccat gatgggcgtg
     1621 ccggtcattt tgatcgacaa gcggccgcgc ttgctggaat ttatcgacgc ccagatcatc
     1681 gacgccctgc aacaacacat gacggacatc ggtgtcacgc tctaccatga agaaggcgtg
     1741 gttacgatcc gaaaagagcc agacggacag atcacggtct cattgacaca tcggccaccg
     1801 atcagcacga cgacactcat gtatgccatc ggccgcatcg gcgcaacgaa gcccttgaac
     1861 ctggaagcca tcggcatcac accggatcca cgcggccgcc ttaccgtcaa tgaacacttc
     1921 caaacatcag tcccccatat ttatgccgtc ggcgacgtca tcggctttcc cgccttggcc
     1981 tccacctcca tgcagcaagg acgccacgcc tcctgccacg cctttaacca tcccgacagc
     2041 accgacacag acctcctgcc ctacggcatc tatgcgattc ctgaaatttc cacggtgggg
     2101 cggaacgagg aagaactggc caaggcaggg attccctatg gggtcggcat cgcacactat
     2161 cgggaaatcg cccgcgggca gctcatcggc gatgaaaccg gtatggtgaa actgctcttc
     2221 catcgccaca cccgcgcgct catgggcgtc catgccatcg gagaaggggc cacggagctc
     2281 attcacatcg gccaggcggt catggcgttc cagggaaaga tcgactactt catcgatacc
     2341 atcttcaatt acccaacctt ggcagaatgt tatcgagtcg ccgcgctgga cggcatcaat
     2401 cgtttgccga ggccttgggc gccgcacgaa tgaaattcgt gacgcgaatc tcgtactgta
     2461 tcgaggaacg tgcggcacac actttacgcc gggcgcttat ctaacaagag gtatgcacat
     2521 gtcattttct gaccatttgc gtaaggtggc ccaaccgata tgggacgcgc agctcaccca
     2581 tccgttcgtc gtcgcgttgg gcaaggggac cttgccggaa cgcaaattca agtactacat
     2641 cctgcaagac gcgagatttc tcggcgacct cgcccgtgtg ttttctgccg cagcgatgag
     2701 agcgccggat tcggagtcgg ccttgcgtct cacaaggctg gctgaggaaa ccattgtcgt
     2761 cgaacgcagc ctccatgaag ggtacggatc acgctggaaa atgagtgcca aagagatgac
     2821 ggctgtgccc atggcgccga cgaactacgc ctatacgcga cacatgctca ctgtcgccca
     2881 cacaggctcg gcagcagagg tcacagtcgt cgcactcccc tgcgcctgga tctactgcgt
     2941 cgtcgggcaa catctcttga aacagggccc gccaaaacac aatcatccct accgggactg
     3001 gctcatgctc tatgcctcac cggagtttgc cgaagtgcag cgctggatga ggagcaaagt
     3061 cgatcagtgg gccaagacag cgggcaatgc agaaaagcgg cggatggaag agtcgttcgt
     3121 cgccagttcg cgatatgagt ggatgttctg ggagatggca tggaacgagg agaagtggcc
     3181 ggtgtgagga gagggagggc tagggctggg aggctctctc cgctcgcagc cacagagcgc
     3241 cgttgaccgc tctaagcttc gcctccactt ggcgccgaaa acttgccaca ccagcgctcc
     3301 gcgaaggtgt ggcttccaac attcgtcgcc actgcctacg gcagccgttg cagctcagca
     3361 agagcggtaa cccaacgacg ctctgatgac gcttcgagag cctcccagcc ctggccctaa
     3421 aataggacac cggccgaaag ggagagaagg gggaaaagaa atgaacgggt cctgtcacga
     3481 atctgggtac ccgttcgtcc tcgcactccg cccggtgtag ggaccccgct gcgggcgaac
     3541 gggtacccag attcgcgcca cccgtcgagt cacaacgtgc cagattgggg ggacaaaaag
     3601 atgggatcgg atagaggggc aggaccgtct cgtgtgctcg cagagccctc atgatgaaac
     3661 agtggtcgct ctgatcacgc tctgagagcc tcccagcccc ggccttctgt gatgtgggac
     3721 acaccggcaa agaagagagg ggaaggaaat gaaaaggcag gcagcggggt cggggttgct
     3781 atgctctctc gctggacagg tcatgcacac tgagtataat gtgcgagagc ctcgaacttt
     3841 ctccctatct caaaggattg ctccccggga atgaatcgtc ttttgatcat ggtcctgatt
     3901 ggcgtctcgg ctctctggac acttccggct tatgcggggt tgtcgtatga ggagagtctg
     3961 aagcaactgg cggagggtgt gatcgctgat gcggtgaaag cgaagagggg gcggctggca
     4021 ctggtggact ttacggacgc caagggcacc gtcacaccga tagggagatt tttagcggag
     4081 gaattgggca cacagattct ggtcgccggc gaactcaaag ttgccgatcg gaccatagtg
     4141 ggctcgacgt tgaaaaaatt ccacgtcacg caattcgaac cggctcaggc tcacgcggtc
     4201 aagcaagccg ccaaggccgt ggatgccgat gtatttgcga ccggctcgta tctggaatca
     4261 ccggacggtt tgcgagtgac ggtgaagcta ctgagcccca gcaccgttca gtcgttaggg
     4321 gccacacgcg gcactcttcc taaaaccggt ccgctggcgg aactgattaa ggagacgaat
     4381 aagccgcctg tcgtaaagat ggatctctcc gccaagccgg cgccaccgcc tggtctcggg
     4441 ttccatagta atgaacagta tcagctggtg gtgaccgccc tgcacaagcg aaatacacag
     4501 ctctcagccg atctcacaat cgagaataaa tcttcgcgca acatcaaagt cctgtgcctg
     4561 ctgcaagaca ccgtgctcga agatgatcaa ggtacgcagt ggaagttggc ggtggagaac
     4621 aatcgcgaag ggctctgcat gcgcggcatc gaactctctc cacgagcaaa agtcatgacg
     4681 gctctgacgt tctcggctcc ggctgacgca cacgagagcc aactgatact tcat
//
LOCUS       DATIPF010000223         5893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1635394, whole genome shotgun sequence.
ACCESSION   DATIPF010000223 DATIPF010000000
VERSION     DATIPF010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635394"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..931
                     /locus_tag="VLL06_05670"
     CDS             290..931
                     /locus_tag="VLL06_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30487.1"
                     /translation="MNRRIIVSVLAVAWVLSLHSLSVSAEGTAQGETSDEKGQQGLGL
                     TDGSQSSNIILGGPDIVLGQIVKIQGEQFSVKGDRGQEISLRVTKDTNKICGEGAGTN
                     VSTGQDSANEQQEIPPTSYMDQQAAQGGKVLSEQDMLKQVHEGSRALSNDPNTLKEVV
                     GSTDPNAKEDTARGSGFMVGGRDCTFKEGDRVRIEASDMGTATTIKQLQHAMK"
     gene            980..1411
                     /locus_tag="VLL06_05675"
     CDS             980..1411
                     /locus_tag="VLL06_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30488.1"
                     /translation="MKSTRKILLLVSWAFVLCVGLPNLAQAINNLSEELEEQQRKQEA
                     QEQGGVPGLAEKDANAAKGITTIHGEILQFDRDQYLIRKYDGDIVRLHIDDKTHVSGE
                     GERSGSGADDSIRPITAHPFDGPCTNLRYNFVAKGLPQFVQ"
     gene            complement(1520..3673)
                     /gene="glgX"
                     /locus_tag="VLL06_05680"
     CDS             complement(1520..3673)
                     /gene="glgX"
                     /locus_tag="VLL06_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HSS30489.1"
                     /translation="MRVLPGRPYPLGAGWDGEGVNFALFSENATAVELCLFDQSDQAQ
                     ESHRIRIEERTDQVWHAYLPEVRPGQMYGYRVHGPYEPMAGHRFNPSKLLIDPYAKAL
                     SGTVRRSDAMFAYPIGDLQADLVRDDRDNAASIPKSVVIEQAFTWGSDRLLQTPWERT
                     IIYEVHVKGLTMRHPEVPEHLRGTYAGLASPAVVEYLQNLGVTAVELLPVHHFVSDKV
                     VTDRGLSNYWGYNTIGFFAPDVRYAVSHDWGRHVNEFKTMVKTLHSAGIEVILDVVYN
                     HTAEGSHLGPTLSFRGIDNATYYRLVPDQPRYYMDFTGCGNTLNVRHPRTLQLIMDSL
                     RYWVREMHVDGFRFDLAAALARELHEVDRLSAFFDTIQQDSVLQQVKLIAEPWDLGEG
                     GYQVGNFPAGWAEWNGKYRDTIRRYWKGDGGQVAELAYRLSGSSDLYETGGRRPHASI
                     NFVTAHDGFSLNDLTTYNQKHNEANGENNRDGTDDNLSWNCGAEGPTDDPAIVELREQ
                     QKRNMMTTLLFSQGVPMICGGDEISRTQRGNNNAYCQDNELSWFDWKLDRRARELFAF
                     TRQLISIRREHPVLRRRQFFQGRRIRGSEVKDLVWFRPDGKEMTDDDWSQGYLRSLGL
                     RLGGDAIEERDEKGRPIQDDTLLLLLNAHHEPLPFTLPAHKRSVRWLMLLETSETERI
                     TKRGTLLRGGTLLTLHARSVAVLREERGVRPGSKS"
     gene            3846..5741
                     /gene="treZ"
                     /locus_tag="VLL06_05685"
     CDS             3846..5741
                     /gene="treZ"
                     /locus_tag="VLL06_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HSS30490.1"
                     /translation="MRREEGWELDLGARPLDSSRVHFRVWAPHAKQVSINLMGQSELA
                     IRPPVQMNPNERGYFELMVSGVEPGARYRYVLDGQKERPDPASRFQPEGVHGSSEVID
                     PEAFQWSDDGWAGIPFHQLIIYELHVGTFTREGTFQAIIPFLEYLRHDVGITAIELMP
                     VAQFPGRRNWGYDGVYPFAVQASYGGSDGLKTFVNTCHQKGIAVVLDVVYNHLGPEGN
                     YLGDFGPYFTDRYRTPWGGAINYDGPESDEVRAYFINNALYWVTEFHIDALRLDAIHG
                     IFDFGACHILRELTEAVHAQADRLGRAIHVIGESDLNDARVISPMVEGGYGLDGQWND
                     DFHHALHTVLTGERRGYYEDFGRLDQLATALCEGFVYSGQRSTFRRRHHGNSSKSRPL
                     SQFIAFSQNHDQVGNRAQGERLSTLVPHEALKVAAATYLLAPKTPLLFMGEEYGERAP
                     FQYFTDHEDPALVEAVRKGRRAEFASFAWDSDVPDPQNPGTFEHSRVYPGNQTLVEHN
                     QILRWYHRLIHLRKSIPALGSAQCGQHEHRVWAEESKQLLFLHRTLHRQPAALIILSF
                     NREPISACLQKPIGTWELMMDSQDVEFGGSGLNGFPATLVIGLKVQTISVPAYGVAVY
                     LDRTPSA"
BASE COUNT         1395 a         1554 c         1596 g         1348 t
ORIGIN      
        1 cgcgtcaggc gcatcctccc agtccggccg ggtgtcgagc agcgcgactt gcaggcggca
       61 catgggcaag caggcaggtc cggcaagata atctttcagc aaggagggaa gacccatgcg
      121 ctatatcagc gtcatatgtg gagtcgtcgc cgcgctcgct ttcggttcga tcttctactt
      181 ctagattcca ggaggaaact cgtaaagttt ccaattgagc ctgaagaaaa gtaattcctg
      241 tgccgaaacc cagcgaccag gagcgaagaa atccaatagg aggtgaggca tgaatcgaag
      301 aatcatcgta tcggtattgg cggtagcatg ggtcctcagt ctgcacagct tgtcggtttc
      361 agctgagggg acggcgcaag gtgagacgtc cgatgagaag gggcagcaag gccttggtct
      421 cactgatggg agtcagtcct ccaatattat tttgggcgga cccgatattg tgctgggaca
      481 gatcgtgaag attcagggag agcagttctc agtcaaagga gaccgggggc aggaaatcag
      541 cctgcgggtg accaaggata ccaataaaat ctgcggggag ggcgcgggga ccaatgtgtc
      601 gaccggacaa gacagtgcga acgagcagca agagatccct ccgacttcct atatggacca
      661 acaagctgct caagggggta aggtcttgtc tgagcaagac atgctgaagc aagtgcatga
      721 agggagccgt gccctttcaa atgatcccaa tacattaaaa gaggttgttg gctcaacgga
      781 cccgaatgcc aaagaggaca cagcgagagg gtctggcttc atggtcggag ggcgggactg
      841 tacattcaaa gaaggcgatc gagtacgcat tgaggcatcc gatatgggaa cggctaccac
      901 gatcaaacaa ttgcagcacg caatgaagta acacgatttc ttccgcatgc aatattcaag
      961 gagattccca tgaattccca tgaaatcgac tcgcaaaatc cttcttctcg tgtcttgggc
     1021 ctttgtgcta tgcgtaggat tgcccaattt ggctcaggcc ataaataatt tgtctgaaga
     1081 attggaagaa cagcaaagga aacaagaggc tcaggagcaa ggaggcgtcc ctggtcttgc
     1141 agagaaagat gcaaatgcgg cgaagggcat cactaccatt catggcgaaa tcttacagtt
     1201 tgaccgcgac cagtatttaa tcaggaagta cgatggggat atagtccgcc tgcatatcga
     1261 cgacaagacc cacgtgagtg gcgaaggtga acgatcagga agtggcgctg acgattcaat
     1321 ccgcccaata acagcccacc catttgatgg gccgtgcaca aacttgcgtt ataacttcgt
     1381 cgcgaaaggt ttaccgcagt tcgtacaatg atactttgcc agtccaagag ccgctgtcac
     1441 tcacttccac gtcaggatgc tcaagtagcc gggagccaac aatctgcctg gataattcgc
     1501 cttaggctga gcctgttcac taggattttg agcccggcct gacacccctt tcctcccgca
     1561 ggaccgcgac agatctagcg tggagagtga gcagggttcc accccgcaac aatgtccctc
     1621 gcttcgtaat tcgttctgtt tcagacgttt ccagtaacat cagccagcga acactccgct
     1681 tgtgagccgg cagagtgaat ggcagcggtt catgatgggc attcagaagc aggaggagcg
     1741 tgtcgtcctg gattggtctg cctttttcgt ctctttcttc aatggcatca ccacctaggc
     1801 gtaatcctag agagcgaaga tagccttgac tccaatcgtc atctgtcatc tcctttccat
     1861 ccggacggaa ccagacgagg tccttgacct ccgatcctcg aatgcgtcta ccctggaaga
     1921 attggcgccg cctgagaacc ggatgttccc gtcgaattga gatcagttgg cgagtgaagg
     1981 cgaaaagttc acgagctcga cggtccaact tccaatcaaa ccaactgagc tcattatcct
     2041 ggcagtatgc gttattgttt ccccgttgcg tgcggctgat ctcgtcgccg ccacagatca
     2101 tcggaacccc ctgagaaaat agaagggtgg tcatcatgtt gcgtttctgc tgttctcgca
     2161 attcaacgat agctggatcg tcggtcgggc cttcggctcc gcaattccaa ctgagattgt
     2221 cgtctgtacc atcgcggttg ttctcaccat tggcctcgtt gtgtttctgg ttatacgtgg
     2281 tgagatcgtt caacgagaat ccgtcgtgtg cagtcacaaa attgatgctg gcgtgagggc
     2341 gccgcccacc cgtctcatag agatcgctgc tgccggagag acgataggcc agttcggcca
     2401 cctggcctcc atctcccttc caataacgcc taatggtgtc acggtacttt ccattccact
     2461 cggcccaacc agcaggaaaa tttccgactt ggtacccgcc ttcacccaaa tcccacggct
     2521 ccgcgatgag cttgacctgt tggaggaccg agtcttgttg aatcgtgtcg aagaacgcgc
     2581 tgagacgatc gacttcatgt agctctcggg ccaaggcggc cgcaaggtcg aatcgaaacc
     2641 catccacgtg catttctctc acccaatatc gcaagctatc catgatgagc tgaagggttc
     2701 gaggatgccg tacgtttaga gtgttgccac agcctgtgaa gtccatgtag tagcgaggtt
     2761 gatcaggaac caggcggtaa taggtggcat tgtcgatccc gcgaaacgag agcgttggcc
     2821 ccaggtggct gccttccgct gtatggttgt acaccacatc aaggatgact tcgattccgg
     2881 cactgtgcag cgtcttgacc atggtcttga attcattgac atggcggccc cagtcgtgcg
     2941 acacggcata ccggacgtcg ggcgcaaaaa agccgatggt gttatagccc caatagtttg
     3001 agagtccccg atccgtcacg accttgtcgg agacaaaatg gtgtaccggg agcagctcga
     3061 ctgcagtcac tcccaggttc tggagatatt ccacgacagc gggagaagct aacccggcat
     3121 aggtgccacg aaggtgttcc ggcacttccg gatggcgcat cgtcaaacct tttacatgta
     3181 cctcatagat aattgttctc tcccaaggcg tctgcagtag tcgatcactt ccccaggtaa
     3241 atgcctgttc gatcacgacc gatttcggga tactggccgc gttgtcccgg tcatcacgaa
     3301 ccagatcggc ttgtaggtcc ccaatcgggt aggcgaacat ggcatcggat ctcctaaccg
     3361 taccgctcag ggccttggca tagggatcga tcaatagctt cgacgggtta aaccgatgcc
     3421 cagccatggg ttcataggga ccatgaaccc gatacccata catctgtccg ggccgcactt
     3481 cgggaagata cgcgtgccag acttgatcgg tgcgctcctc gattctgatc cggtgtgact
     3541 cctgtgcctg gtccgactgg tcaaacaggc agagctccac tgcggtggcg ttctctgaaa
     3601 acaatgcgaa gttgacgcct tccccatccc atcctgctcc caatggatag ggacgacccg
     3661 gtaagaccct catatctcct gctctcaaat tcgtgaatta gggtgctttg agtagggtaa
     3721 tcgtgggcgc ccgcttattc aagagtgagt tgatggggca gggcgttgat ccctggcata
     3781 gctatgtaca ctctggcaca ctgaggttga gaggtttttc tgttgaaatt gaaaaggtga
     3841 ggagagtgag aagggaagaa gggtgggaac tggatctggg ggcacggccg ctcgattcat
     3901 ccagagttca ctttcgcgtg tgggcgcctc atgccaaaca ggtgtcgatc aatcttatgg
     3961 ggcaatccga gctcgcaata cgcccacccg ttcagatgaa tccgaacgaa cgcgggtact
     4021 tcgaacttat ggtgtcgggg gttgaaccag gtgcacgtta ccggtatgtg ctggatgggc
     4081 agaaggaacg tccggatccg gcatctcgtt ttcagcctga aggcgtgcat ggatcttctg
     4141 aagtcatcga tcctgaagcg ttccagtggt ccgatgacgg ttgggcggga atcccatttc
     4201 accaattgat tatctatgaa ctgcatgttg gcacgtttac ccgagaggga acatttcaag
     4261 ccattattcc gttcctcgag tacttgcggc acgacgttgg gattacggca atcgaactca
     4321 tgcctgtggc tcaatttccc ggtagacgca attgggggta cgacggggtg tatccctttg
     4381 cagtccaagc cagttatggt ggatctgacg gacttaaaac tttcgtcaat acttgccatc
     4441 agaagggcat cgccgtggtg ctggatgttg tgtataacca tcttggacct gaaggaaact
     4501 atctaggcga ctttggtcca tacttcacgg accggtacag aactccttgg ggtggcgcca
     4561 tcaactacga tggaccggag agcgacgaag tccgcgcgta tttcatcaac aacgctctct
     4621 attgggtcac cgagttccac atcgacgccc tccgattaga tgccatccat ggtattttcg
     4681 actttggtgc ctgtcacatc ttgcgtgaac tgacggaagc ggtgcatgcc caagccgatc
     4741 ggttaggcag ggctattcat gtgatcgggg aaagtgactt gaatgatgca cgagtcattt
     4801 ctccgatggt cgaaggaggc tacgggctgg acggtcaatg gaacgacgac tttcaccatg
     4861 cgttgcatac cgtgttaaca ggagagcgac gaggatatta cgaggatttt ggacgacttg
     4921 atcaattggc gactgccctg tgtgaaggct ttgtctattc cggacagcga tccaccttcc
     4981 gtcggcggca ccatgggaac tcgtcaaagt cccgtccgct ttcccaattt atcgcctttt
     5041 ctcaaaacca cgaccaggtt ggaaatcggg cccagggaga gcgactcagc accttagtcc
     5101 cacatgaagc cctgaaagta gcagcagcga cctatctgtt ggcgccaaaa actccactgc
     5161 tcttcatggg agaagaatat ggagagagag ccccattcca gtatttcact gaccacgaag
     5221 acccggcctt ggttgaagca gtccggaaag gacgacgagc cgaattcgct tcatttgctt
     5281 gggacagcga tgtgcccgac ccacagaatc ccggcacgtt cgagcactca cgggtttatc
     5341 ccggaaacca aacactggtc gaacacaatc aaatccttcg ctggtaccat cggctcatcc
     5401 atctccgtaa atccattccg gcgctagggt cagctcaatg tggtcagcat gagcatcgcg
     5461 tctgggccga agaatcgaag cagctattgt ttctccatcg cacgcttcat aggcagccgg
     5521 cggcccttat catcctgagc tttaaccggg aaccgatctc cgcctgcctg cagaagccga
     5581 ttggaacttg ggagctgatg atggattcgc aagatgtaga attcggagga tctggtctca
     5641 acggttttcc cgccacacta gttatcggac tcaaggtaca aaccatttcg gttccagcct
     5701 acggcgtggc tgtctatctg gaccgcaccc cttcagcgta attccgcagc aaagagccgt
     5761 ctactcagcc agatcgattc tttgacgccc accacggtct tggtagaagt cgtacctcca
     5821 cagcttctcg aacccgtctc aatcaaagga aaactctctg gccaactata ttgatgtaat
     5881 acacaaataa gag
//
LOCUS       DATIPF010000224         5168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_661910, whole genome shotgun sequence.
ACCESSION   DATIPF010000224 DATIPF010000000
VERSION     DATIPF010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661910"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..404)
                     /locus_tag="VLL06_05690"
     CDS             complement(45..404)
                     /locus_tag="VLL06_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30491.1"
                     /translation="MGPTKAVVKENKLYEVMGGKLIKDGFANRQELDDYVNHHYVTLP
                     VLDNAGNPWLLDGKPVYCLRGTRYETVNDERVHLARCPDCGGMGIRSDEFTVESDCIH
                     CTACGHEFDARLEMMET"
     gene            complement(544..1311)
                     /locus_tag="VLL06_05695"
     CDS             complement(544..1311)
                     /locus_tag="VLL06_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSS30492.1"
                     /translation="MATRTTPSKPRASLDRSIERMRKQLAEFAPFLGRGKPTRSLEEF
                     DMETERLIEELLGQASDLLHAYEYAEVGEAAGLVNMTEEAPEDTGIDSQRQSLLQRGR
                     VLESCVSELEARRSVEPKNARIRSMLTGPQIAEHMSTEVRSVSQDDSLREAAQLMQHW
                     KLGSLFVTNSQSYVGVITDSALTRDVVAKGLDPNTTRVKTCMRTPVVTIEADRPIIDA
                     VRMMKEQATRHLAVTQDGAIIGVFSVSNVLRYYSGAV"
     gene            complement(1431..1880)
                     /locus_tag="VLL06_05700"
     CDS             complement(1431..1880)
                     /locus_tag="VLL06_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30493.1"
                     /translation="MATHTLEAMLSHCTAVSLRERQVGNREFDQTSSRLPVGAIPLSR
                     RCEERMEYRGICSYEMLEVINEDSLIIEQGEAIAVNRSTEGMLLFMGIAPHVKQLIEV
                     HTSRFGWGRTANVFEARWVSQVRVESLGDLYLVGCRRIFGPCHYLSF"
     gene            complement(2341..3681)
                     /locus_tag="VLL06_05705"
     CDS             complement(2341..3681)
                     /locus_tag="VLL06_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HSS30494.1"
                     /translation="MSSISLALLIFGVSYLVILTERIHKTIVALCGAAVMIGLGVVSQ
                     EEAFYSHEFGVDYNVVFLLIGMMVIINIVRETGLFEVLAIWAAQRADAKPFRLLVLLA
                     LLTAGLSAMLDNVTTVLLMAPVTLAITKRLELNPIAFLMTEALASNIGGTATLVGDPP
                     NIMIASKAELGYLDFLVILGPIAVLIMAVFMAALWVMFGRNMTVAPHLRTAVLALSSR
                     EAVLDRPFLHRCLWLLGIVNVGFCIHSLIHLEPATIALLGASLFMLIGHARRKQEDAD
                     ALSYLTDVEWKTIFFFIGLFILVGGLVKVGIIQFLADQLVAVTRGNLAGSTMAVLWGS
                     AMLSAAVDNIPYVAAMNPLIVDLARSLHPEISDYETLVHQPDIMPLWWALALGACLGG
                     NGTIIGASANVVIVDIARKAGYPISFWQFFKFGFPVMLGSVLMSAVYLWLLFLR"
     gene            4374..>5168
                     /locus_tag="VLL06_05710"
     CDS             4374..>5168
                     /locus_tag="VLL06_05710"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HSS30495.1"
                     /translation="MSHRHRKFLSAGLAGVLCSTALFGMTAAYADGKNNLHLPGMRDR
                     DRPNTPENSFTRMFPDLPSFDPQTDAAREQAKKLGEKGGPIDALDDLTDPTLSITDPL
                     RSESNPDNPNMTAGVTFFGQFLDHDVTLDPRSPLLGRSNPKNTTNFRTAAFDLDSVYG
                     NGPEGSPELYDTSSGDIKMRVEPIPGSDLVSRKGAMRFDLPRDSNNNAILGDGRNDEN
                     IIISQFHVAMLRFHNAVVDRLRVDPANVGQSAERIFKMAQRQVRWHY"
BASE COUNT         1257 a         1498 c         1261 g         1152 t
ORIGIN      
        1 gatcgtgcac agtctccgag caaggggcta gatcaggtct cccctcacgt ctccatcatt
       61 tccaatcgtg cgtcaaattc atgcccgcag gccgtacaat ggatgcaatc tgattcgact
      121 gtaaattcat ctgatctgat acccatgcca ccgcaatccg ggcagcgtgc aaggtggacc
      181 ctctcatcat ttacagtttc gtatcgagtg ccacggagac aatacaccgg cttcccatca
      241 agcagccacg gattccccgc gttgtcgagc accggcaacg tcacataatg atggttcaca
      301 tagtcgtcaa gttcctgccg gttggcaaac ccatccttaa tcagtttccc ccccatcacc
      361 tcatagagct tattctcctt cacgaccgcc ttggttggac ccatcacgca cctcccccgt
      421 tctgactagg agcgtatgat tcactgtctc tggcacaaaa caagaaacgt gttcaataaa
      481 gcagctctcg gattttgcca aatcattgcg cttcacgtgt tacgaacgac gagcgatgta
      541 tcgttagaca gccccggagt agtatcggag aacgttgctc acagaaaaca ctccaatgat
      601 ggccccatct tgtgtcacgg ccagatgccg tgtggcctgt tccttcatca tgcgcacggc
      661 gtcgataatc ggccgatcag cttcgatggt caccacgggc gtgcgcatgc aggttttgac
      721 acgggtcgta ttgggatcca gccccttggc taccacatcg cgggtcaagg ccgaatcggt
      781 gatgaccccg acgtacgatt ggctgtttgt gacgaacaga gaccccaact tccaatgctg
      841 catcagctgc gcggcctctc gcaggctgtc gtcttgcgac acgctccgta cctcggtgga
      901 catgtgttcg gcgatctgcg ggccggtgag cattgacctg atcctggcat tcttcggttc
      961 gacagaccgg cgtgcttcca attctgacac acagctctcc aacacgcgac cacgctgcaa
     1021 caagctttgc cgctgactat ctatgccggt gtcttccggc gcctcttccg tcatgttgac
     1081 taatcccgcc gcctccccta cctcagcgta ctcatacgcg tgcagcaagt cagacgcttg
     1141 tccgagcagc tcttcgatca gccgttcggt ttccatgtcg aattcttcaa gactgcgtgt
     1201 cggcttgccc ctgcccaaga acggggcgaa ttcggcaagc tgcttccgca tccgttcaat
     1261 gcttcggtcg agagatgcac ggggttttga gggagtagtg cgggtcgcca tggcgtctcc
     1321 tatcgatgta ggtgagcgtg gacgcccgat cttacccttg ctggattgca agggcaagtc
     1381 cctggaagaa cgagcggcat tccttcgggg tgcggaataa cttgatgggg ctagaacgat
     1441 agatagtgac agggaccgaa tatccgtcgg catccgacca ggtacagatc tccgagtgac
     1501 tccacccgta cctggctaac ccatcgggct tcaaagacat tcgcggtccg gccccatccg
     1561 aagcgggagg tatgcacttc gatcaactgc ttcacgtgag gagcaatgcc catgaagagg
     1621 agcatccctt ctgtgctccg attgacagca atggcctctc cttgctcgat gatgagcgaa
     1681 tcttcattga tgacttcgag catctcatac gaacatatac cccgatactc catccgctcc
     1741 tcacacctgc ggctaagagg aatagcccca acaggcagcc tgctggaagt ttggtcgaat
     1801 tctctatttc ccacctgtcg ctcccgcaat gagacagctg tgcagtggga gagcatcgct
     1861 tcgagagtgt gcgtcgccat gaccaagcct ccagtctcat tgagagaagt gctatctttc
     1921 ggttctttct atcgaataga caggagcaaa catcgtacct cgcaagagga agagcggaca
     1981 cgcttcatag agtgtgtttc ggtatattcc acccccttat atacttaggg ggtaaatacc
     2041 tgcgaagggg aacgaattca ctcttcgaga taaaaatctg tatgcatttg attttccagg
     2101 gatccatgta ctgtagccca tgacctacag tacagcgata ttccttcacc cgactggtga
     2161 taactgcgat gaatgactgg agacaccttc gtgagctctg aatagtccac gcgatcacat
     2221 tgcggctcta cctgatgacc cggtaagtcc aatcctgaaa tctcaaccga tgtggttttg
     2281 tgggacaggc tattcaatca gttccatata ggattgtgag cccgttttat ccgagctagg
     2341 tcagcggaga aagaggagcc agagatacac cgcactcatg agtaccgacc ccaacatgac
     2401 aggaaaccca aacttgaaga actgccaaaa tgagatggga tagccggctt ttcgcgcgat
     2461 atccacaatc accacattcg cacttgcacc gataatcgtc ccattccccc ccagacaggc
     2521 tccaagtgcc aacgcccacc acagcggcat gatgtctggc tgatgtacca aggtctcata
     2581 atccgaaatt tcgggatgaa gcgaccgggc gaggtccaca atcaatggat tcattgccgc
     2641 aacgtaggga atattatcca ctgccgctga gagcattgcc gatccccaga gcaccgccat
     2701 ggtcgacccc gccagattcc ctcgcgtcac tgccaccaac tgatctgcca gaaattgaat
     2761 tatgccgacc ttcaccaatc caccgaccag aataaacaac ccaataaaaa agaagatggt
     2821 tttccattca acatcggtga gatacgacaa cgcgtctgcg tcctcctgct tgcgccttgc
     2881 atgcccgatc agcatgaaca ggcttgcgcc aaggagagca atcgtggccg gctcaagatg
     2941 aatgagcgaa tggatacaga accccacgtt cacgatcccc agcagccaga gacagcggtg
     3001 caagaacggt cgatctaaca cggcctcccg tgaactcaat gccaaaaccg ccgttcgaag
     3061 atgaggcgcc acggtcatgt ttcgcccgaa catgacccac agcgcagcca tgaacacggc
     3121 catgatcaac accgcgatgg gacccaatat cacgagaaaa tcgaggtagc ccaattcggc
     3181 cttgctggcg atcatgatat tgggtggatc cccgactagc gtcgcagtcc cgccgatatt
     3241 cgacgcaagc gcctcggtca tcagaaatgc aatgggattg agttccaatc gcttggtaat
     3301 ggccaacgtg accggcgcca tgagcagcac ggtggtgacg ttatcgagca tagccgagag
     3361 cccagccgtc aatagggcca gtaacaccag cagacgaaac ggctttgcgt cggccctctg
     3421 ggccgcccag atggccaaga cctcgaagag accggtctcc cgaacaatgt taatgatgac
     3481 catcattccg atcagcagaa acaccacgtt gtagtcgact ccaaactcat gggagtagaa
     3541 tgcctcctct tgcgacacca cgccgagccc gatcatcacc gccgccccgc acagcgccac
     3601 aatcgttttg tggatccgtt cagtgaggat gaccagatag gagacaccga agatgaggag
     3661 agccaaagaa atggaagaca tctcgtgcag atttccgcct gaagaataac gctggactca
     3721 aaacctttca cgcattatct ggagaccact gctgcaatgc aatcagagtc gtctttttcg
     3781 atcctactca tgtcgagcta gctatagaga acactgcctc tttccttcgc tatgccatcc
     3841 gctctgaaat tgatctcacc ccgtcgcaga ctctcgagaa atatgatctc gatcgtctta
     3901 tcaaaaatcc ccaagtgtga gaccatcaca gcgtgtaaaa accagatgcc gccgccgaat
     3961 tggccaagaa caatgcagcc cgacggcacc ataacagaaa tttgcaattc tcccaatgtc
     4021 ttctctccac ttctgacaga cttggcagta cgtttagttg ccgaacggat cttctatcca
     4081 aggcaatcct gatgcctatg tccgtaacat taggttagga accaggctgt gtgagaagcc
     4141 acgttgtatc tttcttaagg ccgatgacca cattcgaata caatgtgctt ttgatggtgc
     4201 actctctaga cagggctcaa ttcttttatt tcgcctgaaa aaaacacgcc atacgtcttg
     4261 tgcgcttccc acggttcgga tcttgctcct gcctgactgc atctcgttct ggccgggaaa
     4321 ctattgaatc ccggatctca agaagacctg aacatcatca tgaggaggat acgatgtctc
     4381 atcgtcacag gaaatttctg tctgccggtc tagcaggagt gctgtgttct acagcactgt
     4441 tcggcatgac cgcggcttac gctgatggaa aaaataattt gcaccttccc ggtatgcggg
     4501 atagggatcg acccaacact cccgagaatt catttacccg tatgttccct gacttgccgt
     4561 ccttcgaccc gcagaccgat gccgctcgtg aacaggccaa gaagttgggc gaaaaaggcg
     4621 gccccatcga tgcgttagat gacttgaccg atcccactct atcgatcaca gatcccctgc
     4681 ggagtgaaag caatccagac aaccccaaca tgactgccgg cgtgacattt ttcggtcagt
     4741 ttttggacca tgatgtgacg ctcgatccga ggtccccatt gctcggacgg agcaacccaa
     4801 aaaatacgac aaactttcgc accgcggcgt tcgatctgga cagtgtgtat gggaatgggc
     4861 cggaaggatc acctgagttg tatgacacga gttctggcga tattaagatg cgcgtggaac
     4921 caattccagg ttcagacctc gtctcacgaa agggcgcaat gcgttttgac ctaccgcgcg
     4981 attccaacaa taacgccatc ctgggcgacg gccggaacga tgagaatatc attatcagtc
     5041 aattccacgt cgcgatgctg cgctttcaca acgctgtggt tgaccggctc cgcgtcgacc
     5101 cggcgaacgt cggtcaatct gctgagcgga tcttcaagat ggcccaacgc caggtgcgct
     5161 ggcactat
//
LOCUS       DATIPF010000225         5835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1103988, whole genome shotgun sequence.
ACCESSION   DATIPF010000225 DATIPF010000000
VERSION     DATIPF010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103988"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            637..897
                     /locus_tag="VLL06_05715"
     CDS             637..897
                     /locus_tag="VLL06_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30496.1"
                     /translation="MPGPQNPDTFEHSRVHPGIQTLVEHNQILRWYHRLITLRKSILP
                     LESAQSSQHKHHVWIEESEQVLFLYRTLHRQRAALIIRTPSG"
     gene            complement(1004..1447)
                     /locus_tag="VLL06_05720"
     CDS             complement(1004..1447)
                     /locus_tag="VLL06_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSS30497.1"
                     /translation="MPYYYFDSTALVKRYSMERGTRIISRLMVKRSKVAILPTWSVTD
                     FYTVFANRAHHGEITRDDCYSVIHKFERESHEGLFQFITPTMQTYLATKELALEYPAL
                     RSPQVLHLALALELKPLRLTVVSADTQLLTACHSAGLHTINPEED"
     gene            complement(1452..1730)
                     /locus_tag="VLL06_05725"
     CDS             complement(1452..1730)
                     /locus_tag="VLL06_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30498.1"
                     /translation="MAKSKSEAGRTPTRAPRSRGRGLTVTKQRVPSRLAKRRLGERLK
                     NDTASFNQGNLADKFRKEGYDELPLDELQDRLSKMSGPLAAIILKGRV"
     gene            complement(1753..2916)
                     /locus_tag="VLL06_05730"
     CDS             complement(1753..2916)
                     /locus_tag="VLL06_05730"
                     /inference="COORDINATES: protein motif:HMM:NF014248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HSS30499.1"
                     /translation="MRKGSRFVSRIGVVSTMLVVLSGCAWFGGQSKPDWIDGVSAAYP
                     ASQYLVGVGQADSLATAEDRAYAAVARIFKAEVSAQAKDWESYLLIEQRGHSSAERRL
                     TLDNLTRVSTDKVLENVRIVDRWGDVRKGLHYALAGMHRPQAETSFMERITELDRSIS
                     DDVEEAQRPADKLAKVKALRRAARNLVLREAYNADLRVVRPSGQGTAAAYRVSELTHE
                     LEEFLATNLVLAVAVTGDQAEPVQRALTEGLLKEGLQVTSRPLGGDRSVGGDSSGPSP
                     ELLVRGIVRVWPIDVRDPQFKFVRWCSDLEVVDVASHRVVGALSKGGKEGHLSEREAT
                     ARVVRVMQQEFSADVAKSIAAHVYGETELPTQAGQSAGCPRDGLVSAPALAPH"
     gene            complement(2913..4409)
                     /locus_tag="VLL06_05735"
     CDS             complement(2913..4409)
                     /locus_tag="VLL06_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30500.1"
                     /translation="MSPLAPGPTANDIPKRWGCFLSIHSLLAVLALVLLAGCGPSVNR
                     YLLIETSLRANDPKGADAIVQRDEKEYGEKSRVLYGMDRGMTLQLAGDYQQSNAVLEE
                     AEEEVDRLYTRKIRTETLAFMTNDTALAYEGDPYEQVMINVLKALNYAVLGQWQDALV
                     EARRIDHRLNVLSDRTKEKNAYRDDAFARYLSGILYESTGDVNNAFIAYRNAYESFDA
                     TRSWSHTAVPSQLRADLLRTAEALHFTQELVAYRSLFPDTRWETSQALQQLAQVVVIS
                     YNGRAPRKEDQFLDLPISLDALQLVLLNRGFSQSNRQPNRGVDTVLYGLNGHVVRVAL
                     PRLVPQKTQVPVDRVSLVPDDGKPVILNTEVVHNVTALADKALAERMAGITVKALARA
                     ATKFALAEGAMRGAQHAAGKDAGPWVGLLVGLLAKGLAVASEEADKRSWQTLPDEIHL
                     ARVWVPPGHYQVQNRLAGELNDALTPEGMRTLTLGPGETVLFLQRVMP"
     gene            complement(4425..5030)
                     /locus_tag="VLL06_05740"
     CDS             complement(4425..5030)
                     /locus_tag="VLL06_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HSS30501.1"
                     /translation="MMDRGRIGLVALAVVVLAGCAQETRITRVDSEVITDLSGRWNDT
                     DSRMVAETMVKEALEYPWLNNFSGSTHRQPVVVVGTILNSSHEHINVNTFVADLEREL
                     TNSQKVTFVAGKGDRDELRTERKEQAMYAREDTQKAPGKEIGADFMMKGTIATILDEV
                     DGTKAVFYQIDLQMVDLESNAKVWYGQKKIKKVVEKKRSIF"
     gene            complement(5087..5653)
                     /locus_tag="VLL06_05745"
     CDS             complement(5087..5653)
                     /locus_tag="VLL06_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30502.1"
                     /translation="MNRVLGFKSRLVGGGVAIALLVGLAACGGPPKWVKQGSGAFNEK
                     DIKGFYGVGAVAGVRNEPLAWDTAENRARAEIAKSFETYTGYLMRDYAASTTAGDFTK
                     NSEEQNVERAIKTVTTTTLSGVRPIDRYKDEKTNTYYVLTRLNLEEMKENLEKAKELN
                     AQVRDYVRRNADKLFERLEKEEDKRAPR"
BASE COUNT         1237 a         1780 c         1461 g         1357 t
ORIGIN      
        1 ggcggccatc caatgggcca cagggccacc cacgcaacat gggccttaga agtcgagggg
       61 aagcggcgaa ggtctgaaac ggatgctttt tttcttttgc ctctatgcct ctaccactac
      121 cgcatttgtc catttgtccc cgtcaacagg tgcctatcga taaccgcgcg cccagtggcc
      181 tcgagccgat aggtcgcggt gggaatcctc ggatgggaga ttggcattgg atcctcggac
      241 tggttctcgg atcgtttgga gcattcaacg cctctgatcc gctaataatg attgttgacc
      301 acacccaggc cttattcaac cctcttcttt gtgtgttaca tctgccagag cgtttttctc
      361 gtttgagatg gattggagaa gctgtcgagg tacgacttct acgaaacccg tgggggctgt
      421 caaagaatcg atctggctga gtagacggct ctttgctgcg gaattatgct ggaggggtgc
      481 ggtccagata gacaacaacg ccgtaggctg gaaccgaaat ggtttgttcc ttgagtccga
      541 gaactagcga gtactgacca cggagaccca gccttagttg aaacagttcg gaaaggacga
      601 cgggcagaat tcgcctcatt tgcttgggag ggcgagatgc cgggcccaca gaatcccgac
      661 acattcgagc actcacgggt tcatccagga atccaaacac tagtcgaaca caaccaaatc
      721 cttcgctggt accatcggct catcacgctc cgcaaatcca ttctgccgtt agaatcagct
      781 cagtccagcc agcataaaca tcatgtatgg atcgaggagt cggagcaggt attgtttctc
      841 tatcgcacgc tccatcggca gcgggcggcc cttatcatcc gcacgccttc aggctgaaaa
      901 cgggatgccg gatccggacg ttccttctgc ccatccaaca catagcgata acatcgaagt
      961 tgttaatcga cccctacgca aaggtaccca ccatggtctc tcatcaatcc tcttcagggt
     1021 taatagtatg gaggcccgcc gaatgacagg ccgtcagcag ctgcgtatcg gcactgacga
     1081 cggtcagccg caacggcttg agttccaacg ccagtgccaa atgcaatacc tgtggtgatc
     1141 ggagcgcagg atactccaat gcaagttcct tcgtcgctaa gtacgtctgc atcgtcggag
     1201 taataaactg gaagagaccc tcatgtgatt ctcgttcaaa tttatgaatc accgaatagc
     1261 aatcatcccg ggtaatctct ccatggtggg ctcgatttgc gaacaccgtg tagaaatcgg
     1321 tcacggacca tgtcggcaga attgccactt tgctccgctt gaccatcagc ctgctgatga
     1381 tgcgtgtacc gcgttccatg ctgtaccgtt ttaccagagc tgttgagtcg aaatagtagt
     1441 atggcatgcg atcacactct ccccttcagg ataatggcgg ccaacgggcc cgacattttc
     1501 gaaagccgat cttggagttc atccagcggc agctcgtcat agccttcctt ccggaacttg
     1561 tcggccaagt tgccttgatt aaacgacgcg gtgtcgttct tgagccgctc acccaggcga
     1621 cgtttcgcca agcgactggg aacccgttgt tttgtgaccg tcaacccgcg gccacgactt
     1681 cgtggtgcac gagttggtgt acgtcctgct tccgatttcg atttggccat acccttcctc
     1741 tctccttctg aattagtggg gtgctaacgc cggcgcagag accagcccgt cccgcggaca
     1801 tcccgcagac tggcccgcct gtgtcggcaa ttctgtctct ccatacacat gtgccgcaat
     1861 ggatttcgcc acatcggcag aaaactcttg ctgcatgaca cgcacaactc tggccgtcgc
     1921 ctcacgctcc gacagatggc cttccttccc acccttcgac aaggcgccga ccacccgatg
     1981 gctagccaca tccacaactt caagatcgct gcaccatctc acaaatttaa actggggatc
     2041 ccgcacatcg atcggccaca ccctgacaat gccacgcacg agcagctccg gagatggtcc
     2101 gctggaatca ccaccgactg aacgatctcc tcctaacggt cgactcgtca cttgcagccc
     2161 ttccttcagc aatccttccg tcaaggcgcg ttgcaccggt tcagcctgat cgccggtcac
     2221 cgcaaccgcc aacaccagat ttgtggccag gaactcttcg agttcgtgcg tgagttcgct
     2281 cacacggtag gctgcggcgg tgccctggcc actcggacga accactcgaa gatcagcgtt
     2341 gtaggcctcg cgcagcacca gatttctggc agcccgtctc aatgctttga cttttgccag
     2401 cttatcagct ggccgctggg cctcttcgac atcatcgctg atcgatcgat ccaactcggt
     2461 aatccgttcc atgaacgagg tctctgcctg aggccggtgc atccctgcca aggcatagtg
     2521 gagccctttg cgcacatcac cccatcgatc aacgatccgg acgttctcca atactttgtc
     2581 ggttgataca cgagtcagat tgtcgagggt caaacgtcgt tccgcactgc tgtgtccgcg
     2641 ctgctcaatg agcaagtacg actcccagtc tttcgcctgc gcgctgactt cggctttaaa
     2701 aatacgggcc accgctgcat aggcgcgatc ctcggctgtc gcaagactgt ccgcctgccc
     2761 cacgccaacg agatattgac tggcaggata ggcggcgctc acgccatcga tccaatccgg
     2821 tttcgattgc cctccaaacc atgcgcagcc ggacagcaca accaacatcg tcgacaccac
     2881 cccgattcga ctcacgaacc gcgacccctt tctcatggca tcacccgttg aaggaacagg
     2941 accgtctcgc caggcccaag cgtcagggtc cgcatgcctt caggtgtcag cgcgtcattt
     3001 aattcaccgg ctaatcgatt ctgcacctgg taatgcccag gtggaaccca gacccgcgcg
     3061 agatggattt catcgggtaa cgtctgccag ctccgcttat cggcttcttc cgatgccacc
     3121 gccaaccctt tggctagcag ccccacgaga agaccgaccc atggccccgc atccttcccc
     3181 gctgcatgct gagcccctct catcgctccc tcagccagcg caaacttcgt ggcagcacgg
     3241 gccagagcct tcaccgtgat tccagccatt cgttccgcta gcgctttatc tgccaacgcc
     3301 gtcacgttat gcacgacctc cgtattcagg atcaccggct tgccatcatc cggaacgaga
     3361 cttaccctat cgacaggcac ctgcgttttc tgcggaacga gccgtggcaa ggccacgcga
     3421 acgacatgtc cgttaagccc gtataagacc gtatccacac cccgattggg ctgccgattc
     3481 gactgcgaga atccgcggtt gaggagcacc aactggagtg catcgagact gatcggcaag
     3541 tcaagaaact gatcctcctt gcgtggtgcg cggccattat agctgatcac gacaacttga
     3601 gcgagttgtt gcaatgcctg actggtctcc catcttgtat cgggaaatag agatcggtag
     3661 gcgaccaatt cctgtgtgaa atgcaatgcc tcggccgtcc gtaatagatc tgcccgcagc
     3721 tgggacggta cggccgtgtg cgaccacgaa cgggtggcat caaaactctc atacgcattc
     3781 cgatacgcaa taaacgcatt gttcacgtcg ccggtgctct catagagaat gccgctcaga
     3841 tagcgagcaa aggcgtcatc ccgataggca tttttctctt tggttcgatc cgacagaacg
     3901 ttgagccggt gatcgatacg ccgggcctcg acgagcgcat cttgccactg acccaacacg
     3961 gcatagttga gcgccttcag gacattgatc atcacttgtt cgtaggggtc gccctcatag
     4021 gctaatgccg tgtcattcgt catgaacgcg agggtttcag ttcgaatctt tcgagtatag
     4081 agacgatcca cttcctcctc agcttcttcc aatacggcgt tgctctgctg atagtcgccg
     4141 gcaagttgaa gggtcattcc gcgatccatg ccatagagca cacggctttt ctcgccatat
     4201 tccttttcat cccgctggac aatcgcatca gcgcccttgg ggtcattggc ccgaaggctc
     4261 gtttcaatga ggagatagcg gttgaccgag gggccgcatc cggcaagtag caccaacgcc
     4321 aggacggcta agagggaatg gatggaaaga aaacagcccc accgttttgg aatatcgttg
     4381 gcggtggggc ctggggcaag agggctcaac ggaggtgttg gagcctaaaa gatggaacgt
     4441 ttcttttcga cgactttctt aatcttcttc tgaccgtacc agaccttggc attactctcc
     4501 aaatccacca tctgcagatc gatctggtag aaaaccgcct tggtcccatc tacctcatcg
     4561 aggatcgtcg caatggtacc tttcatcatg aaatctgcgc cgatttcctt gcccggcgct
     4621 ttctgcgtat cctctcgtgc atacatggct tgctcttttc gctcagtgcg cagttcatca
     4681 cgatctccct ttcctgccac aaaggtgacc ttctgcgagt tcgtcagttc ccgttcaagg
     4741 tcggccacaa aggtgttcac gttgatatgt tcatggctgc tgttcaggat ggtcccgacg
     4801 actaccaccg gctggcggtg ggtcgaaccg gagaagttgt tgagccacgg atactccagc
     4861 gcctctttga ccatcgtctc tgcgaccatc ctggaatcgg tatcgttcca gcggccgctc
     4921 agatctgtga tgacctcgga gtcaaccctc gttattctcg tctcctgggc acagccggcc
     4981 aacaccacta ccgccaatgc gacaagcccg attcgtcctc gatccatcat aatcacgcga
     5041 tacctcctcg gaaatgcagc gattggcggt cgatcagtca gagcctttac cggggtgccc
     5101 gtttgtcttc ttctttctca agccgctcaa agagcttatc ggcattccgt ctgacgtagt
     5161 cacgaacctg ggcgttgagt tccttcgcct tctcgagatt ttccttcatc tcctcgagat
     5221 tcagcctggt caacacatag tatgtgttgg tcttctcgtc cttgtagcga tcgatgggac
     5281 gaacaccgct gagggtcgtg gtcgttacgg tcttaatggc ccgttcaacg ttttgctctt
     5341 ctgagttttt cgtaaaatct ccggccgtcg tggaggccgc atagtcgcgc attaagtagc
     5401 cagtataggt ttcaaagctc ttggcaattt cagctcgggc acgattctca gcggtgtccc
     5461 aggccagcgg ctcgttccga acaccggcta ccgccccgac accataaaag cctttaatgt
     5521 ccttttcgtt aaacgcccct gacccctgtt tcacccactt cgggggaccg ccgcaagcag
     5581 caagtccgac caatagggcg atagccactc ctcccccgac caaccttgac ttgaacccca
     5641 ataccctatt catgtgcatg cctccttggt aatagtcggg acggtaatac ggctgcgcac
     5701 cagcggtagg tgccaatgac acgcgttcgt gaaattgcgt gaattcatgc taacatgcac
     5761 ccccaattcc gtcaacgaaa ccgacggctg atggttggcc gtcttattct tccaccaacg
     5821 gaggataccc gtggc
//
LOCUS       DATIPF010000226         4809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_130625, whole genome shotgun sequence.
ACCESSION   DATIPF010000226 DATIPF010000000
VERSION     DATIPF010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130625"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VLL06_05750"
     CDS             <1..256
                     /locus_tag="VLL06_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30503.1"
                     /translation="IADVSDAPEILERAIAFVSQFSGTDFQSRRFLGGLIGYVRAYHP
                     DIAVYLTKQREPGHIILPGQQFMPPPAAEPHTHGPGCHHH"
     gene            241..615
                     /locus_tag="VLL06_05755"
     CDS             241..615
                     /locus_tag="VLL06_05755"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS30504.1"
                     /translation="MSPPLTESAPDNTRPHTMTGEKERKIRKARRVELRCTLGFSSGE
                     VEGDATITNISTAGCRAESTINMAEGLDVQVALQLPGQSSPVKVERASVRWVSGHAFG
                     LSFVLFFPSERARLRTFIEKIK"
     gene            complement(638..1258)
                     /locus_tag="VLL06_05760"
     CDS             complement(638..1258)
                     /locus_tag="VLL06_05760"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSS30505.1"
                     /translation="MRRDAVVMNITILCGTLLVAGLLVGCGGKTDMTPITPPPSPGPR
                     AVIKTKFGDIHIKLYPDVAPNHVKNFIQLAKSGFYNGTIFHRVIPGFMIQGGDPNTKN
                     SLHKELYGQGGPKDENGNPVLLKAEFSDIPHKRGIVSMARANEPDTAGSQFFVVVESS
                     HFLDGKYTAFGEVTKGIGVADKIVMMPRNDRDLPNERVEMTVTILE"
     gene            complement(1280..1918)
                     /locus_tag="VLL06_05765"
     CDS             complement(1280..1918)
                     /locus_tag="VLL06_05765"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSS30506.1"
                     /translation="MRSVIIPGRQMMERRIFVKMVAVTCVLGGSLLLGMGTLFAADKA
                     PVGKAEMTKGPKATIKTKFGDMDIVFFPEKAPKHVENFMTLAKSGFYNGTIFHRVIPG
                     FMIQGGDPNTKDLNKPETYGQGGPSQRLKAEFNDIPHRRGILSMARTSDPNSAGSQFF
                     IVVKDSNFLDGQYTVFGEVVKGMEVADKIVSLPRNSQDLPTDRAEMTVVVVE"
     gene            complement(1948..2655)
                     /gene="rnc"
                     /locus_tag="VLL06_05770"
     CDS             complement(1948..2655)
                     /gene="rnc"
                     /locus_tag="VLL06_05770"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HSS30507.1"
                     /translation="MTSASSADSLQSLLGYRFKVLGLLEEALTHSSLVNEQKSVSLQH
                     NERLEFLGDAVLSLVVSEYLASTLPQSSEGALSKLKAQLVSESSLAQVARRLGLGEYL
                     KLGRGEDRSKGREKDSLLADALEAVLAAVHLDGGFDASRTVTRRIFAEELTHIVAQQE
                     QPGAGDYKTQFQEWCQKRHDTLPRYATVRETGPDHQKSFEVELSIQGEVVGVGSGRSK
                     KEAEQQAAKQALQQLGV"
     gene            complement(2686..3936)
                     /gene="fabF"
                     /locus_tag="VLL06_05775"
     CDS             complement(2686..3936)
                     /gene="fabF"
                     /locus_tag="VLL06_05775"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HSS30508.1"
                     /translation="MSVRSTRRIVVTGLGVITPLGTGVDKTWKAICAGESGIGRITKF
                     DPADYDAQIAGEVKDFDPAQFIEKKEIKKMDTFIHYAVGAAQMAVDDAGFSVAPEEAT
                     KVGVYIGSGIGGLGSIEHYHKILQEKGPGRVSPFFIPMTIINLASGQVAIRLGAKGPN
                     SCAVTACATGNHCIGDAFRLIQSGDADVMIAGGAEAAITPLGVAGFAASKALSFRNDD
                     PTKASRPFDKDRDGFVLGEGAGVVVLEELEHARRRGVRIYAELIGYGMNSDAYHITAP
                     SEDGEGAVRCMELALKDAGIGKDQVGYINAHGTSTMADATETKAIKQVFGERAFRIPV
                     SSTKSMTGHLLGAAGGIEAVFSVLALHHGILPPTINLDHPDPACDLDYIPHRARPVAV
                     AVALSNSFGFGGVNACLLFKKPGV"
     gene            complement(3951..4184)
                     /gene="acpP"
                     /locus_tag="VLL06_05780"
     CDS             complement(3951..4184)
                     /gene="acpP"
                     /locus_tag="VLL06_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HSS30509.1"
                     /translation="MGTVDERVKKIIGEQLGVEEDEVTPEASFVEDLGADSLDTVELV
                     MALEEEFGIEIPDEDAEKILTVGKALDYIKEKA"
     gene            complement(4277..>4809)
                     /gene="fabG"
                     /locus_tag="VLL06_05785"
     CDS             complement(4277..>4809)
                     /gene="fabG"
                     /locus_tag="VLL06_05785"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HSS30510.1"
                     /translation="MVDQVLKEWGKVDILVNNAGITRDGLLLRMKEEDWSLVLQVNLN
                     GTFNCTKAVLLPMTRQRYGRIVNIASIVGVMGNVGQANYAASKAAVIGLTKTVAREYA
                     SRSVTVNAVAPGFIDTAMTQGLSAEVKDALQKQIPLGRLGTPADIAAAVHFLVSDEAA
                     YITGHVLHVNGGMLMS"
BASE COUNT         1046 a         1515 c         1149 g         1099 t
ORIGIN      
        1 gatcgccgac gtatccgatg caccggaaat actggaacgt gccatcgcgt tcgtgtccca
       61 attttctggt acagatttcc aatcacgacg gtttctcggt gggctcatcg gctatgtgcg
      121 tgcctaccac ccggacatcg cggtatacct gaccaagcaa cgtgaaccgg gccacatcat
      181 tctgcccgga cagcagttca tgccgccccc ggcggctgaa ccacataccc acggaccggg
      241 atgtcaccac cactgacgga atccgctccc gataacacta ggcctcacac catgaccgga
      301 gaaaaagaac gaaagatcag aaaagcacgc cgagtggagt tacgctgcac cctgggattt
      361 tcttccgggg aagtggaagg cgacgccacg attaccaaca tctcgacggc cggctgccgg
      421 gcagaatcta cgattaatat ggcggaaggg ctcgacgttc aggtcgccct tcagcttccc
      481 ggccagtcat ccccggtgaa agtcgagcgg gcatcggtac gttgggtctc aggccatgcc
      541 tttggcctga gcttcgtgct gttctttccg tccgagcgag cccgcctgcg cacgttcatc
      601 gaaaaaatca aatagcattg tcccctccac actcctctta ttcgagaatc gtcaccgtca
      661 tctcaacccg ttcattcggc aagtcgcgat catttcttgg catcattacg atcttgtctg
      721 cgacaccaat gcctttggtc acctcgccaa aggccgtgta tttcccgtcg aggaaatggg
      781 acgactccac gacgacaaaa aactgtgacc ccgcggtatc cggctcgttc gctcgtgcca
      841 ttgacacaat accgcgcttg tgaggaatgt cgctgaactc cgccttcaat aagaccggat
      901 taccattctc atccttcggc ccaccctgtc catacagctc cttgtggagc gagttcttcg
      961 tattgggatc gccgccctgg atcataaaac ctggaatcac tcggtggaaa atcgtcccgt
     1021 tgtaaaaacc tgacttggcc agctggataa agttcttcac atgattcgga gccacatccg
     1081 gatacaactt gatatggatg tccccgaact tcgtcttgat aacggccctt ggaccagggc
     1141 ttggcggagg agtaatgggg gtcatatcgg tcttcccacc acaaccaacg agaagaccgg
     1201 ccaccaacag cgtgccgcat agtatggtga tgttcatgac aacagcgtca cgcctcatag
     1261 cccaccaccc ttgcatcggt tactcgacca ccacaacggt catctctgcc cgatcggtcg
     1321 gaaggtcctg gctgttcctt ggtaagctga caatcttgtc cgccacttcc atccccttca
     1381 ccacttcacc aaagaccgta tattgcccat cgagaaagtt cgaatccttg acgacaatga
     1441 agaattgcga accggcacta ttgggatcgc tcgtacgtgc catagagaga atgcctcgtc
     1501 tgtgagggat gtcgttgaac tccgccttga gtcgctggct gggcccccct tggccgtaag
     1561 tctccggctt attcaggtct ttggtgttgg gatcgccgcc ttggatcata aagcctggga
     1621 tgactcgatg aaagattgtg ccgttataga agccggattt cgccaatgtc atgaagttct
     1681 ccacatgctt gggggccttc tccgggaaga acacgatgtc catgtcacca aactttgttt
     1741 tgatagtggc tttaggacct ttcgtcattt ctgctttccc aactggagcc ttgtcagcgg
     1801 cgaagagggt acccatcccc agcaatagac tcccaccgag cacacacgtc actgccacca
     1861 ttttcacgaa gattcgtcgc tccatcatct gcctccctgg aataataact gatctcatgg
     1921 cgcagtcatc ctatcacggt gaccggatca aacccctaat tgttggaggg cctgtttcgc
     1981 cgcctgttgt tctgcttctt tcttgcttcg gccggatccc acaccgacga cttctccctg
     2041 aatcgacagt tccacctcaa atgatttctg gtggtcgggt cccgtttccc gaacggtcgc
     2101 atagcggggc aatgtgtcat gccgcttttg acaccactcc tgaaactgtg tcttgtagtc
     2161 gcccgctccc ggctgttcct gttgtgcgac aatatgcgtg agttcctctg cgaagatgcg
     2221 gcgcgtcacc gtacggctgg cgtcgaatcc accgtcaaga tgaaccgctg ccaacaccgc
     2281 ctccagcgca tcggccaaca aggagtcttt ttcacgtccc ttcgaccggt cctctccgcg
     2341 tccgagtttg agatactctc ccagtccaag cctacgggcg acctgcgcca atgatgactc
     2401 actcactaac tgtgctttca acttcgagag agctccctct gaagactgcg gcaacgtgga
     2461 agccagatac tcgcttacca cgagcgacaa cacggcatca ccgagaaact caagccgctc
     2521 gttatgttga agcgacaccg atttctgttc attgacgaga gaggagtgcg tcaaggcttc
     2581 ttcgagcaag ccaagaacct tgaatcgata gcccaacaag gactggagtg agtcggcgga
     2641 ggaggccgaa gtcatagcgg cgagtacacc cctcgcacag gtctgttaga cgccgggctt
     2701 tttaaagagc agacaggcat tgaccccgcc gaacccaaaa gaattcgaca gtgcaaccgc
     2761 gactgcaaca ggcctggccc tgtgcggaat gtagtccaga tcgcacgcag gatccggatg
     2821 atccagattg attgtcgggg gtagtatccc gtggtgaagc gccagaacgc taaacactgc
     2881 ctcaatccca ccggccgccc caagtaaatg gccggtcatc gacttggtcg aactgaccgg
     2941 aatgcggaag gctcgctcac cgaatacctg cttgatggct ttggtctcag tggcatctgc
     3001 catcgtcgac gttccatgcg cattgatgta cccaacctgg tccttgccga tacctgcatc
     3061 cttgagagcc aattccatac accggaccgc accttcacca tcttctgacg gggcggtgat
     3121 atggtaggcg tcgctgttca tgccgtaacc gatgagttcg gcatagatcc gaacccctcg
     3181 tcgacgtgca tgttccagct cctccaggac cacgactccg gcgccttcgc caagtacgaa
     3241 tccatcgcga tccttgtcga acggtcggct tgctttcgtc gggtcatcgt tacgaaacga
     3301 caaggctttc gacgcggcaa accctgcaac ccccaacggt gtaatggcag cttccgcccc
     3361 acccgcgatc atgacatcgg catcaccgga ttgaatcagt cgaaacgcgt caccgataca
     3421 gtgattgccc gtcgcacagg ctgtcaccgc acaggagttc ggccctttgg ctcccaatct
     3481 gatcgcgacc tgcccggagg ccaggttaat aatggtcatc ggaataaaaa acggcgacac
     3541 acgccccggg cccttttcct ggagaatctt atggtagtgc tcgatggatc caagcccccc
     3601 gatgcccgac ccgatataca caccgacttt cgtcgcctct tccggcgcga cgctgaaccc
     3661 tgcatcatcc accgccatct gggcagcgcc cacggcataa tggatgaacg tatccatctt
     3721 tttgatttct tttttttcga tgaactgggc ggggtcaaaa tctttgactt caccggcgat
     3781 ttgggcatcg taatctgcag ggtcaaactt cgtgattcga ccgatgccgg attcgccggc
     3841 acagatggcc ttccacgtct tgtcgacacc cgtccccaga ggcgtgatca cccccaggcc
     3901 agtcacgaca atccgtctgg tcgaacgaac actcatgacg tcgatgactg ctaggccttt
     3961 tccttaatat agtccaatgc tttcccgacc gtcagaatct tctccgcgtc ttcgtcggga
     4021 atctcgatgc cgaattcttc ttcgagggcc atcaccagct caaccgtatc aagcgagtcg
     4081 gcaccaaggt cttccacaaa gctggcttcg ggcgtgacct catcttcttc gactccaagt
     4141 tgctccccaa taattttctt cactcgttca tccaccgtgc ccattgattt tcctgcctcc
     4201 ttccccatcc tagaccctcc ttatcgctgc aagcctgcca gccacagcat cgtacaataa
     4261 tctacccctc catccgttag gacatcaaca ttcccccatt cacgtgcaac acatgcccgg
     4321 tgatataggc ggcctcgtcc gacacgagaa aatgcaccgc cgccgcaata tcggccggtg
     4381 tgcccaaccg tcccaacgga atttgttttt ggagcgcgtc tttcacctcc gccgacaacc
     4441 cctgtgtcat ggcggtatcg atgaatcctg gcgcgaccgc gttcaccgtc accgaacggc
     4501 tcgcatattc ccgcgcaacg gtcttggtca atccgatcac cgccgctttc gatgccgcgt
     4561 aattggcctg gcctacattg cccatcaccc cgacaatcga cgcaatgttg acaatacgac
     4621 cataccgctg cctggtcatt gggagcaaga ccgccttcgt gcagttgaac gtcccattca
     4681 aattgacttg taacaccagg ctccagtctt cctctttcat ccgcaacaac aacccatcac
     4741 gcgtaatccc agcattgttc accagaatat cgaccttccc ccattccttg agtacctggt
     4801 cgaccatgg
//
LOCUS       DATIPF010000227         6359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_926956, whole genome shotgun sequence.
ACCESSION   DATIPF010000227 DATIPF010000000
VERSION     DATIPF010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926956"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VLL06_05790"
     CDS             <1..710
                     /locus_tag="VLL06_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSS30511.1"
                     /translation="VARDLFRALREGGASFFADLVRSTSHLPSEVENGLWELVAAGLV
                     TADGFDNLRALMDPHRRRAEGRERARRTRHAAGRWSLLRTATSHQPSAVSSAESVARQ
                     LLRRYGVVFRDLLGREVLALSWRDLLVQYRRMELQGEVRGGRFVGGFTGEQFALAEAV
                     ESLRAVRKQHEMSPGSHDIKLSASDPLNLAGVILPGPRVPAVPSNFVVIKDGVIVRTV
                     IGRGGDAQRIDQAQVAR"
     gene            complement(879..1274)
                     /locus_tag="VLL06_05795"
     CDS             complement(879..1274)
                     /locus_tag="VLL06_05795"
                     /inference="COORDINATES: protein motif:HMM:NF022666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HSS30512.1"
                     /translation="MTRPGYILSSLCSAVGLILTLSGCSITETINDILSSTSGRSWFT
                     EDGLVKADHRVDAFMTLNFENVKQDMARGQGEYLSSLSTLMRVPQDREASFFVHAQSR
                     YPFVIEHQSSPEEVIALLRSTLPTATALR"
     gene            complement(1485..2312)
                     /locus_tag="VLL06_05800"
     CDS             complement(1485..2312)
                     /locus_tag="VLL06_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSS30513.1"
                     /translation="MLPFRRIFHPTDFSAADSAAFAHAVKLTILVQGELTMMHVDPTI
                     ERKHFEDFPRIRPLLARWEILPEGSSKDDVAKLGVQIKRVRAVADNTTGALLQQLTTH
                     PTELLVLSTHQHAGLARLTHHGVAEPLARGTHARTLFLPVGVEGFISAETGRTNLTRV
                     LIPIAHTPHPQPAIDLAADLASTLGNETVLFELLHIGKESDLPKFTHPERPGWSWNML
                     VGKGDPVEWILAAGRDFDADLIVMMTEGHTSLFDLLLGSTTERVVRGTRCPLLAIPT"
     gene            complement(2338..2718)
                     /locus_tag="VLL06_05805"
     CDS             complement(2338..2718)
                     /locus_tag="VLL06_05805"
                     /inference="COORDINATES: protein motif:HMM:NF022666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HSS30514.1"
                     /translation="MKRVTVWVIFPMLILIAPGCTLKGTVKEITDTTSNITGTTSGRT
                     WFTEDGLLHPDHKLTAFMALNQMNVEQDLARGHGEYATSLGTLLGLPNNQQTNFHAKA
                     QEAFETLLTSDPEARREQLRLLAR"
     gene            3054..4049
                     /locus_tag="VLL06_05810"
     CDS             3054..4049
                     /locus_tag="VLL06_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30515.1"
                     /translation="MSAQGLTQVDPQQFFRLPSTNATGAQRLDTKVSGVAVSNDFSGR
                     LSVTTTEGDTITLSADLESDFRAVNYTSHSEGEGQAVDVRAKYAEYSLKQEFGVTIEG
                     DLNEQEVKDLEKLFRKVANIFKQYVSGQDKEALAKTAKLAERFGHLSSLSGLDFSVDV
                     ERSVTIFAAQAASEVTNPPPRVTDQQQQTPVMTSGTATPGVAPSTVAAIPQSSTGTSA
                     PTQPSTDAVVPTSAAATRPIAPAQNAAHPTSLVQQVLDALQASKVESHKVHKYLPDFF
                     NKLREDLYDELRGHREQTPRTPAAGPSESSATTISSALFAYQSVNLTSISLSIRT"
     gene            4269..5096
                     /locus_tag="VLL06_05815"
     CDS             4269..5096
                     /locus_tag="VLL06_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30516.1"
                     /translation="MTKQVPPRIYCIPATDAPVVAVFRRGPSNWAHVGRWDLAVGRYE
                     PGAWLGGRIFPWRSDLSPDGQYLCYFAHKPSATWEHGEAYVAIAKLPWLTALHSFSTC
                     GTWTRGYYFTQDSGSDEPQDPPLPIPYGLRSIPAIQFANERRCGWIEASDSPVRDPTD
                     AWDQHRNARMRKSQPQGTRVLCAESVGWAGGEFGIDQAVDGLRVRYALECNGNIELLD
                     DFQWADWSREGHLLVALRSGQIQIRSLEGDRPTILFEADLSNLEPNPTPAPRWAQNW"
     gene            complement(5286..5648)
                     /locus_tag="VLL06_05820"
     CDS             complement(5286..5648)
                     /locus_tag="VLL06_05820"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS30517.1"
                     /translation="MQPRCGLRIPVGYPASIQSDTGAGEGILLDLSPTGCRMRSDIAL
                     NAGTYLALQIAVAQEPTPLAVEVSVVRWCKDGHVGVEFLRYSQGVRERVTDLVKALPP
                     TEIHVRPDYAATILSAVS"
     gene            complement(5761..6111)
                     /locus_tag="VLL06_05825"
     CDS             complement(5761..6111)
                     /locus_tag="VLL06_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30518.1"
                     /translation="MSLHKALILAEQVTYERINGRVASTSELLQLVLSDPWFTWLHPL
                     SQLVIRIDELLDDESELLLVEVEHFLIEARALIRPSEEGDGFERSYYEALQREPDVIF
                     AHVEVKKLLTKVAA"
BASE COUNT         1377 a         1649 c         1915 g         1418 t
ORIGIN      
        1 atgtggcgcg cgatcttttc cgagccttgc gcgagggtgg agcaagcttc ttcgccgatc
       61 ttgtgcggag caccagccat ctgccgtcgg aagtcgagaa cggactatgg gagctggtcg
      121 cggcaggatt ggtgacggcg gatggctttg acaatttgcg cgccctcatg gatccacatc
      181 gtcggcgggc agaagggcga gaacgtgcac gccgaactcg ccatgcagcg ggccggtggt
      241 ccttgcttcg aactgctacc agccatcagc catcggctgt cagttcggca gagagcgtcg
      301 cgcgccagtt gttgcgtcga tatggcgtgg tgttccgcga tttgttgggg cgggaagtgc
      361 tggcattgtc ctggcgcgac ttgctggtgc agtaccgccg gatggaactg caaggcgagg
      421 tccgtggtgg gcggtttgtg ggcggcttta ccggggagca attcgccttg gcggaggctg
      481 tcgaatcgct acgggcggtg agaaaacagc acgagatgag tcctggctct catgacatca
      541 aactgtctgc cagcgatccg ttgaaccttg caggagtcat cttgcctggc ccccgtgtgc
      601 cagcagtgcc atcgaacttc gtggtgatca aggacggcgt cattgtacgg acggtgatcg
      661 ggcgaggagg cgacgcgcaa cggatcgacc aagcccaggt agccaggtag ttcgtagcgg
      721 acagtgaccc agcgtgtggg ctttcgttgt catcagatct ggtcacccat tcaggtggag
      781 actgcgacac ccaagctcct gtccattcta acagacaatg acagacgaga gtttgtagag
      841 cgaagctcca tcagtccact tgagaaatcg ttctgcggtc aacggagcgc cgtggccgtc
      901 ggcaacgtgc tacggagcag tgcgatgacc tcctcggggc tactctgatg ttcaatcaca
      961 aatggatagc gggattgagc gtgaacgaaa aacgaggcct ctcgatcctg cgggactctc
     1021 attaacgtgc tgagcgacga aaggtattca ccctgtcctc gcgccatatc ctgttttacg
     1081 ttttcgaagt tgagcgtcat gaaggcatcg accctgtggt ccgccttgac cagtccgtct
     1141 tctgtaaacc aagacctccc cgacgtggaa gacagaatgt cattaatcgt ttcggtgatg
     1201 ctgcaacccg acaatgtgag gatgagcccg acggcagagc agagagaact caggatgtat
     1261 cccggtcgtg tcatggctac ctcctagata gtgaaatgaa ccgagcatga atcgtagtca
     1321 aggtgatgtt ctactgtaga gcaggccagc ggcttgatca agttatgaat cgagatgggt
     1381 tatagcagcg atcctcgccg gcgcagtttg gcctcgacta ggctccggtc caggctgtga
     1441 acagaccgaa cgagcagatt aagtgggcgg acggatcagt cagattaagt aggaattgca
     1501 agcaagggac agcgtgtacc acggaccacc cgttcggtgg tgcttccgag aagcagatcg
     1561 aataggctgg tatgtccttc ggtcatcatg acaatcagat ctgcatcgaa atctctccct
     1621 gccgctaaga tccactcaac gggatcgccc ttcccaacga gcatgttcca cgaccagccg
     1681 gggcgctcgg ggtgagtaaa ttttggaaga tctgattctt tcccaatatg cagtagctcg
     1741 aataaaactg tctcgttgcc cagtgtgctc gccaagtctg cggcaagatc gatggccggt
     1801 tgggggtgtg gggtatgggc aatcgggatc aacacacggg tcaggttcgt tctgcccgtc
     1861 tccgctgaga taaatccttc gacaccgacc gggagaaaga gcgttctggc atgggtaccg
     1921 cgcgccaagg gctcggcaac cccgtgatgg gtgaggcgag cgaggcctgc gtgctggtgc
     1981 gtcgacagga cgagcaactc cgtggggtgg gttgtcagtt gttgaaggag ggcgcccgtc
     2041 gtattgtctg cgacggcacg gacgcgtttg atctggacgc ccaatttcgc gacatcgtct
     2101 ttcgaactgc cttccggcaa aatctcccag cgagcgagga gcggccggat gcgagggaaa
     2161 tcctcaaagt gttttcgttc gatcgtcggg tcgacgtgca tcatcgtcag ttctccctgc
     2221 acaagaatgg tgagcttgac ggcatgagcg aacgccgccg agtctgcagc ggagaaatca
     2281 gtcggatgaa aaatccggcg aaatgggagc atcaatacgt cgttcgttgt ttcgtagcta
     2341 gcgagccagc aaccgcaatt gttcacgccg ggcttcagga tcggacgtca agagcgtctc
     2401 gaatgcctcc tgcgcttttg cgtgaaagtt tgtttgctgg ttgtttggca gccccaggag
     2461 ggtgccgagc gatgtcgcat attcaccatg ccctcgcgcc aagtcctgtt ccacattcat
     2521 ctggttcaag gccatgaagg cggtgagctt atggtctgga tgcaaaaggc cgtcttcggt
     2581 gaaccaggtg cggccggagg tcgtgcctgt gatattggag gtagtatccg tgatttcttt
     2641 gactgtgccc ttcagtgtgc agccgggtgc gatgagtatg agcatcggaa atatgaccca
     2701 aacagtgacc cgtttcatat tgtacctcct cggtcggaag gggccggctc tcttcgtgat
     2761 tgactgatcc cactatatca caagcctatg acccacattg tccatgacgg gtcatggcaa
     2821 aatcgtagaa ccgcgtcgcg agaccggcgc gcggagccgt gagagagagg catacttgaa
     2881 actgtttgtt gaccgaccga ggggcgagtc cgttggtcaa aggctgtcat agcagcggat
     2941 cggtagttgg agcagaaggg ctcatgagtc atatgggtaa agttctgcag gatgtatacc
     3001 gatagagagc tgtgcgtacg cggtagtgat cctattcgtc ctggagacgc gtcatgtccg
     3061 cccaaggtct gactcaagtc gatcctcagc agttttttcg tcttccatct acgaatgcca
     3121 cgggggccca gcgattggat accaaggtca gcggagtcgc agtctccaac gacttctccg
     3181 gccggctgag tgtcaccacg acggaaggtg acaccatcac cctctcggcg gatcttgagt
     3241 ccgatttccg tgcggtgaac tatacatccc atagcgaagg cgagggccag gcggtggatg
     3301 tgagggcgaa gtatgccgag tattcactga aacaagagtt tggcgtgacg attgaaggtg
     3361 atctgaacga gcaagaggtg aaggatctgg agaaactgtt cagaaaagtc gccaatatct
     3421 tcaagcaata tgtcagtgga caggataagg aggctctggc caagacggca aagctggccg
     3481 agcggtttgg acacctgtca tcgctctcgg gtctggactt cagtgtggat gtggaacggt
     3541 ccgtcacgat tttcgccgca caggctgctt cggaggtgac taatcctcct ccgcgagtga
     3601 cggaccagca acagcagaca cctgtcatga catctggcac agctaccccg ggtgtggcgc
     3661 cttcgacggt tgcggcgatc ccgcaatcgt ccactggtac ctcggcgccc acccagccat
     3721 cgactgacgc agtggttccg acatcggccg cggcgacccg tcccattgcg ccggcgcaga
     3781 atgcggcgca cccaacgtct ctcgtgcagc aagtcttgga cgcactgcaa gcatcgaagg
     3841 ttgaatcgca caaggtccac aagtatctgc cggacttttt caataagctg cgcgaggatc
     3901 tatatgacga actgcgcggt catcgtgaac agacgcctcg gactcccgct gccggaccat
     3961 cggagtcctc cgccactacg ataagcagtg cgctgtttgc ctatcagtcg gtgaatctga
     4021 cctcgatatc cctatcaatc aggacctaat ataacagcgt cagccacagg gctggcgtta
     4081 agcaagacac ggcgaggtgt ggtgatccca cgcctcgcca ttgtcgttct ttcggccaat
     4141 gatgttacgc attctagacg caggatggcg tacctcgtac ttgatgcggc cgagacatgt
     4201 gatagtgttt gtcgtaggat agacgggtca atgttggtgc cgtcacaaat acgcatgaat
     4261 gccaggtgat gacgaagcag gttcctcctc ggatttactg cattccagca actgatgcgc
     4321 cggtggtggc ggtatttcgc cggggtcctt ccaattgggc ccacgtgggg cggtgggatt
     4381 tggctgtggg gcgatacgag cccggtgcgt ggcttggcgg gcgcatcttt ccgtggcgat
     4441 cagacctttc tccggatggg cagtacctct gttacttcgc tcacaagcct agtgccacgt
     4501 gggagcatgg cgaggcctac gtcgccattg caaagcttcc ctggcttacg gccctccatt
     4561 ctttcagcac gtgcggaacc tggacgcgcg ggtattactt tacccaagat agcgggagtg
     4621 atgagccaca ggacccgccg cttccgattc cgtacggctt gcgttccatt ccagctattc
     4681 aattcgccaa tgagcggcga tgcggctgga tcgaagcgtc ggactctcct gtccgcgacc
     4741 ctacagatgc ctgggatcag catcgcaatg cccgcatgcg gaaatcgcag ccgcaaggca
     4801 cccgtgtgct atgtgccgaa agtgtaggat gggcgggagg agaatttggt attgaccaag
     4861 cggtggacgg tctgcgtgtt cgctacgcgc tggaatgcaa tggcaacatc gaattattag
     4921 atgactttca atgggcggat tggagtcgag agggacatct gctggttgcc ttgcgttctg
     4981 gacaaataca aattcggagt cttgaaggcg atcgcccgac aatcctgttc gaagcggacc
     5041 tgtcgaatct ggaaccgaat cccacaccgg cgccccgatg ggctcagaac tggtagcgac
     5101 tgtgtcggtt tgcatgaatc tctttgcttg gaccgtggtg gtgagcgctc actgggggga
     5161 cggaatcatc tcgacaaata caatacattg gggtcaagtc ttgccatgcg atagtgaaag
     5221 gcagagagag cgccacgtga atctttacct acagtgggga cagggtctct gtgcgggaag
     5281 ctgtatcagc tgacggcgct tagtattgtt gctgcatagt cagggcggac atgtatttct
     5341 gttggtggca gggctttgac aagatctgtc acccgttcgc ggaccccctg actgtaccga
     5401 agaaactcta ctccgacgtg gccgtcttta caccagcgga ccaccgatac ttctactgcg
     5461 agtggggttg gctcctgagc caccgcgatc tgtagtgcga gataagttcc tgcattcagc
     5521 gcgatgtcac ttcgcattct gcatccggtg ggcgagaggt cgagtaagat cccttctcct
     5581 gcaccggtat cgctttggat actggcaggg tagccgactg ggattcgaag accacatcgt
     5641 ggctgcatcg cgagaacctc cgttctgagt atacaaaaca tatcggaaga ggcgaggaaa
     5701 acttgaataa cggtcgacgg aagtgaggca gaattcagat gaaccagaag ctggtgagga
     5761 ttaggcagcg actttggtga gaagtttttt gacctcgacg tgggcgaata tgacgtcggg
     5821 ctcacgctga agagcttcgt aataacttcg ttcgaacccg tctccttcct ccgaaggacg
     5881 aatgagggct cgagcttcta tcagaaaatg ctccacctca acaagcaaga gctcggactc
     5941 gtcatccaag agttcatcaa tgcgaatcac cagttgagat aacgggtgaa gccaagtgaa
     6001 ccacggatcg ctcagcacta attgcagcag ttcgctggtt gatgcgactc gaccgttgat
     6061 gcgctcatag gtcacctgtt ccgccaggat gagagccttg tggaggctca agagactgct
     6121 ccgcatatcg tggagtctct tgcggtgagg gcaagatttc tcggtcgaag tcttttgcga
     6181 tgccgtttcc ataaaataac tttagcaggt tattaaaaaa atggtgaccc tcggctcggt
     6241 gcacgtctcg atcccaggta tcatgtgaat atcggcatat tcaccggtgc actgtagcag
     6301 tgcttatgaa aaagtcctca ggctttctga aaagctaagg cccgggtcaa ggctgaggt
//
LOCUS       DATIPF010000228         3480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1368631, whole genome shotgun sequence.
ACCESSION   DATIPF010000228 DATIPF010000000
VERSION     DATIPF010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368631"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..958)
                     /locus_tag="VLL06_05830"
     CDS             complement(206..958)
                     /locus_tag="VLL06_05830"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HSS30519.1"
                     /translation="MKKDKTASLTGKVAIVTGASNGIGRAIAERVAQEGATVVVNYGK
                     SADKAKDVVAGIEANGGKALAIQADMSRVADVGRLVKDTVKTFGRLDILVNNAGMFLY
                     KPLVETTEEEFDNMFALNTKGPYFAMQEAAKVIHEGGRIVNISTDGTHIGFAGATSYL
                     GSKGALEQFTRGLAHELAPKGVTVNTVSPGYTATDMLPADPTFRQIGEQASPLKRLGV
                     PKDIADVVAFVVSEEARWLTGQNIHAGGGVVM"
     gene            complement(1035..1277)
                     /locus_tag="VLL06_05835"
     CDS             complement(1035..1277)
                     /locus_tag="VLL06_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30520.1"
                     /translation="MASVLEFTGDRSIMAGDWSSPLVRSKNHCPRCGGLMVHEWGADL
                     MGHASQRCVLCGELIDSVILENRRLQQAGTSSLGTK"
     gene            complement(1499..>3480)
                     /locus_tag="VLL06_05840"
     CDS             complement(1499..>3480)
                     /locus_tag="VLL06_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSS30521.1"
                     /translation="QLLQDLAQRLPQIVPFDYMNVILHEPTRNVMRLWLLVTSTPSTI
                     SPGLELPIDESPGGLVWKNQVPCLVEDVATDTRFPKLMAMLRENGVQSFGVVPLTTAQ
                     RRLGAMAFGSLQRRTYQEEEITFMQQVAKQVAVAVDNVLHAASALAAQQQLQYERDRV
                     QLLLEVNNAVVSHLDLRDVFTAVSGCLRKVIRHDGSALVLFDSTTNRCRVHVLDFAKQ
                     EDFVEDLPITPECKSPSDKAITTKKPVVYRKQDLEILAAESEITRQLLDQGVRSFCSV
                     PLLSHERVLGALNVGRTDDAEFTPAEIELLSQVAQQIAIAVDNGLAYQEIAQLKDTLT
                     KEKLYLEEEIQTEYNFEEIIGDSAILKRTLKQVQTVATTDSTVLILGETGSGKELVAR
                     ALHNLSNRRERTFVKLNCAAIPTGLLESELFGHERGAFTGAIATKAGRFELADRGTLF
                     LDEVGEIPLELQVKLLRVLQEQEFERLGSTRTIRVNVRVIAATNRDLPQLVEEKQFRN
                     DLYYRLNVFPVTVPPLRERTGDIPLLVRHFAQKLAQRMKKRIEAIPSDSMQALQQYAW
                     PGNVRELENFIERAVILTQGPDLFVPLAELKRAPKSSSHSAATLEQAEREHILKILRA
                     TNWVVSGTAGAASKLGMKRTTLQSKMQKLGISRLR"
BASE COUNT          671 a         1031 c          967 g          811 t
ORIGIN      
        1 gcgccgcgtc gtcgaggagg ccaagggccg tgtggtcgcg cccatacttc catgcgatca
       61 aaccgatacc cgatgtgatc accattgcga gaagcagagc cgatccgatg acattacgac
      121 gattcatgcg tcacaccctc cctcagatcg cagcccaaca gtccttctgt ttcgagatgg
      181 tctttcatga gtgtctcctt gccgattaca tcaccacgcc gccgccggca tggatgttct
      241 ggccggttaa ccaccgcgcc tcttcactga cgacaaacgc cacgacgtcg gcgatatctt
      301 tgggcacgcc gagtcgtttg agcggtgagg cctgttcgcc gatttgacgg aaggttggat
      361 cggcagggag catgtccgtt gccgtgtatc cgggagacac cgtattgacg gtcactccct
      421 tgggcgctag ttcatgcgcg aggcccctcg tgaattgttc gagcgcacct ttgctcccga
      481 gataggaggt ggcgccggca aaaccgatat gggtgccgtc ggtggaaatg ttcacgatgc
      541 gtcccccttc gtggatgacc ttcgccgcct cttgcatcgc aaaatacggc cccttggtat
      601 tgagggcaaa catgttgtcg aactcctcct cggtcgtttc gaccaggggt ttgtacaaga
      661 acatgccggc gttgttcacc agaatgtcca gccggccgaa cgtcttcacc gtatccttga
      721 ctaaacggcc gacgtcggcg acccggctca tatcggcctg gatcgcaagg gccttgccac
      781 cgtttgcctc gatcccggcg acgacgtcct tggccttgtc cgcactcttg ccatagttca
      841 ccacgacggt ggctccttcc tgggcgactc gttcggcaat cgcacggcca attccgttgg
      901 atgctccggt cacaatggca actttacctg tcaacgatgc tgtcttgtcc tttttcatgg
      961 tcatgtctcc ttattggtgg tctcgccgct cggctggcta gccttcagat tccctcctcc
     1021 ggggcgcggc tcgattattt cgtgcctagg ctggatgtac ctgcttgctg aagcctgcga
     1081 ttctccagga tgaccgaatc gatcagttca ccgcagagca cgcagcgctg gctggcatgg
     1141 cccatcagat ctgcccccca ttcatgaacc atgagcccgc cacagcgcgg acagtgattc
     1201 ttgctgcgaa cgagcggcga gctccaatct cctgccatga tcgaacggtc tcctgtgaac
     1261 tcaagtactg aagccatcga tccctccttg ctctacggtc tgtgatcttg ccttacccca
     1321 ttcatgacaa catgctatgg caacttgcat tccagccaag actccacccg atgccattcg
     1381 ctgatacctt tgaaactaga ggagattgcg cgatcttgat cggggattct tgtgtgagaa
     1441 gaaagaatgt gacggggacg gactgccgaa gtatcggcac cttgccgagc aatcggcact
     1501 aacgaaggcg ggagatcccg agcttctgca ttttggattg gagagtggtg cgtttcattc
     1561 caagtttgct cgcggcaccg gcagtgccgc tcaccaccca gttggtggca cggagaattt
     1621 tcagaatgtg ttcccgttcg gcctgttcca atgtcgccgc tgagtgactc gaactctttg
     1681 gtgcccgttt caattcggcg agcgggacga acagatcagg tccctgggtg aggatgaccg
     1741 cccgctcgat aaagttctcc aattcccgca cattgccggg ccaggcgtac tgctgcagcg
     1801 cctgcatgga gtccgacggg atcgcctcga tgcgtttctt cattcgttgt gcgagctttt
     1861 gggcgaaatg ccggacaagc aaggggatgt ccccggttcg ctcgcgcaac ggcggcacgg
     1921 taaccgggaa tacgttgagg cggtagtaca agtcgttgcg gaactgtttt tcctcgacca
     1981 gttggggcaa atcgcgatta gtcgcggcga tcacacgcac attgacgcgg atcgtgcggg
     2041 tgctccccaa tcgttcaaac tcctgctctt gcagcacacg aagaagcttg acttgtaact
     2101 ccaacggaat ctcccccacc tcgtcgagga agagggtccc gcggtcggct aattcgaacc
     2161 ggccggcctt ggtggcgatg gctccggtaa acgcaccccg ttcatgtccg aagagctcgc
     2221 tctccaacaa tcccgtcgga atggcggcgc aattcagttt gacgaacgtc cgttcccgcc
     2281 ggttgctgag attgtgaagc gcccgtgcaa ccagctcctt cccgcttcct gtctcgccca
     2341 gaatcagaac ggtggaatcg gttgttgcca cagtttggac ttgtttgagg gtgcgcttca
     2401 gtattgcgct gtctccgatg atttcctcga agttgtactc cgtttggatc tcctcctcga
     2461 gatacagttt ctcttttgtc aatgtgtctt tcagctgagc aatttcctga taggccaatc
     2521 cattgtccac cgcaatcgcg atctgttgcg cgacctggct caggagttcg atttcagccg
     2581 gcgtgaattc ggcgtcgtcc gtacgtccca cgttcagtgc accgaggact ctctcgtggg
     2641 acaacagggg aacggaacag aaagagcgaa ccccctggtc cagcagctgt cgggtaatct
     2701 cggattcggc tgccagtatt tcaagatctt gcttccgata gaccactggc tttttggtgg
     2761 tgatcgcttt gtcagatgga gacttgcact ctggcgtgat cggcaagtcc tcaacaaaat
     2821 cttcctgctt cgcgaagtcc agcacgtgca cacggcagcg attggtcgtg gaatcgaaca
     2881 agacgagtgc cgacccatcg tgccggatca cttttcgtag acagccgctg accgcggtga
     2941 acacgtcccg taagtccaag tgcgacacca ccgcgttgtt gacctcaagc agcagctgca
     3001 cgcgatcccg ttcatattgc agttgttgct gggcagcgag agcactcgcc gcgtgcaata
     3061 cgttatccac ggccaccgcg acttgcttcg ccacctgctg catgaaggtg atctcttcct
     3121 cctgataggt cctccgctgg aggctcccaa atgccatcgc ccccagacgg cgctgcgccg
     3181 ttgtgagggg aaccacacca aacgattgga cgccgttttc gcgcagcatg gccatcagtt
     3241 tcgggaagcg ggtgtcggtc gccacgtcct caaccagaca gggcacctga tttttccaga
     3301 ccaatccccc gggagattcg tcgatgggca gttcaagccc ggggctgatt gtggagggcg
     3361 tcgaggtcac caagagccac aggcgcataa cattgcgcgt tggctcatgc aaaattacgt
     3421 tcatgtaatc gaatggcacg atctggggga gccgttgagc gaggtcttga agcagttggt
//
LOCUS       DATIPF010000229         5532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1636146, whole genome shotgun sequence.
ACCESSION   DATIPF010000229 DATIPF010000000
VERSION     DATIPF010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1636146"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..704)
                     /locus_tag="VLL06_05845"
     CDS             complement(84..704)
                     /locus_tag="VLL06_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HSS30522.1"
                     /translation="MRILAIGAHPDDIEAGCGGTLLKYAQKGHRIFLMVMTAGDKGGS
                     SGVRKREQEQSAKQLGAEKIFWGGYEDTAVPMGRELIQEIEEVVKEVQPHFIFSHYHD
                     DTHQDHRHLAMSTITATRYTKNVLFYEGPTTQNFSPTVFVDIDQVVEEKIKSIEAHAS
                     QVKKTNIEGLSIVDVIRAGAHFRGIQGRVKNAEGFVPLRLFINIGA"
     gene            complement(753..1466)
                     /locus_tag="VLL06_05850"
     CDS             complement(753..1466)
                     /locus_tag="VLL06_05850"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HSS30523.1"
                     /translation="MRVTIHQPQFLPWLGYLDKIDQADLFIALDSVQFKKNEWQNRNR
                     IRTSDGCQWITVPVLHRFGQLVSEVCINQAVPWRSQHLRAIEMHYARAPFRDRYLPEL
                     RRLYETPWERLHDLNMAVIRWLLRAFGVTTPVRCASEWAAREEPTDRLIDLCHAVGAS
                     GYLSGPGGEQYLDTVQFESSGLKLEIQQFQHPMYRQVYEPFMPAMSAIDLLFCCGPAA
                     LARLREARAGQNVVTAVQD"
     gene            complement(1476..3029)
                     /locus_tag="VLL06_05855"
     CDS             complement(1476..3029)
                     /locus_tag="VLL06_05855"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HSS30524.1"
                     /translation="MTDAGWGVLIFATVLLCLCCAVGHAQASDDRQQLLATVHVHSTA
                     STGDLTIEALAERAEQLGLNVLILTDNFVLRYEYGLWPLPGLLKRQVRFPSVLEYGIE
                     RYVEEIAAAQIRHPNLVIVPGIEVAPYYYWTGSLWGRTLTMHNAQRNLLVFGLTEPED
                     YRSLPVSGNPGSYTFDWRGVINGAPILLAVPAVWLWRPLRRASDRQSLRRTTACILIV
                     LAGASVVYSWPLSQPAFSSYDDRLGYRPYQALIDDVRAKGGLVFWSMTEARDFREHAA
                     GPLGTVIIKTEPHPEALVQTTGYTGFGGLYQDGRTIVKPGELWDRLLQVPAQEQRQIP
                     VLIGELAFHGLNDAGKDLHRLVMGVQVKQRTVAGVLEALQSGHSYAVGEGDHHVQLRL
                     EEFRIVCQGGSKSASAGDRLDPGMERDLVIRLAVTASDRGRHPVKVRVIRSGQVLVQL
                     AGETPFLIDWPDTTVPTAERLTYRVEITGSGELLSNPIVVGPILGPNAVSSEPSMSSE
                     KPFNSKIES"
     gene            complement(3043..>5532)
                     /locus_tag="VLL06_05860"
     CDS             complement(3043..>5532)
                     /locus_tag="VLL06_05860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSS30525.1"
                     /translation="RGMNIVLLAEVTVEQVIGGAERVLRQQALGLAKLGHDVRVMTRA
                     PFESAAAELTIGTVTERRYAVTRTSEPAFVRSSVRNSLRVFDLMRQTSPIDIALVHQS
                     LAGLGPILFRYGMVGRWVYVCHSLAHEEYLTRAERAATTLEGVRRRLSVRARRWIERT
                     VIRRCHHVIVLSEFMKRQVVAAHGIAPQHITVVPGAADPVTFHPVDDRNEIRRELKLP
                     EGRTLMFTVRNLVPRMGLEYLLEAMTMLGEEQQNLMLLIGGEGQLRPRLEELIRSRGL
                     QDSVQLLGFVPEEQLAKYYQASDLVVLPTLQLEGFGLVTVEALACSTPVVGTPVGAIP
                     EVLSRVDPMLVTEGTDGVSLANTLRLVLNRFREIPHERERVSKKGREAVEQRYNWVAH
                     TADLDHLFMNLLKDRPVASHQAFRNLSNSGPSASTNGMVSPAPKSRRVIHVITRLDHG
                     GSAQNTMLTALGHDRAQFEPIVVAGHPGCWDAQGGQLATEENCRKLEKAAIRWVLLPS
                     LTRELHPIKDAQALWQLIRLFRHERPALVHTHTSKAGVLGRIAAWITRVPVIVHTPHG
                     HVFYGHFGPFQSWLFLQIERVLSVITDRLIALTDAERQDHLDRSVGRADRFAVVPSGI
                     DRERFGRARAQGKQQPDWFGCPPDALVVGSVGWLTDIKGHEYLITAVAKLKQDFPSLH
                     LVIIGSGDRYDALSRQAESAGLHDSVHLLGHRDDVEACLAGMDLFVLPSLNEGMGRAL
                     IEAMAAGLPVIASRVGGIPAVISHERTGLLVPPRDADALAEALRRLLDRPEWAKQLGV
                     AASHSVDSRYGSASMVHAIESIFAEALSAHL"
BASE COUNT         1218 a         1769 c         1429 g         1116 t
ORIGIN      
        1 ggaacgaagg acttcggccg tcgctcgcaa gtcttcctcg ccgatcgcag ggcgtgagtg
       61 gggaatgatc gtggccacag ttgtcatgcg ccgatattaa taaacaatcg aagcggaaca
      121 aagccttctg cgtttttcac ccggccctga atgccgcgga aatgcgcccc ggcacgaatc
      181 acatccacaa tactcagacc ttcgatgttg gtctttttca cctgcgaggc atgggcctcg
      241 atcgatttga ttttttcctc gacgacctgg tcgatatcga caaagaccgt cggcgagaaa
      301 ttctgggtcg tcggtccctc gtagaacaac acattcttgg tataacgggt cgcggtgatc
      361 gtgctcatgg ccaggtgccg atggtcctga tgggtatcgt cgtgatagtg agagaagatg
      421 aagtgcggct gaacctcctt cacgacctct tcgatctcct gaatcaactc acgccccatc
      481 ggaaccgccg tgtcttcata cccgccccag aaaatcttct cggctcccaa ttgtttcgcc
      541 gattgttcct gctcacgctt gcggaccccg ctcgaaccac ccttatcgcc ggccgtcatc
      601 accatcagaa aaatacgatg acccttttgc gcatacttga gcaacgttcc gccgcagcca
      661 gcctcaatat cgtccggatg ggcgccgatt gccagaatcc gcatcggtat tcttcgttcg
      721 ctcgccattt ttccccctcg tatccccttc gtttagtcct gcaccgccgt gaccacattc
      781 tgtcccgcgc gtgcctcgcg caatcgcgcc agcgcggccg ggccgcagca gaacaacaga
      841 tcgattgccg acatcgccgg catgaatggt tcgtagactt gccggtacat gggatgttgg
      901 aactgttgaa tctccaactt caatccggac gattcaaact gcacggtgtc cagatactgt
      961 tccccgccag ggccggaaag gtatcccgat gctccaacgg catggcacag gtcaatcaaa
     1021 cggtcggtgg gctcttcgcg tgccgcccac tccgatgcac agcgaacggg cgtcgtcaca
     1081 ccgaacgccc tgagcagcca acgaatgacc gccatattca aatcgtgcaa ccgctcccat
     1141 ggcgtctcat agaggcgacg caactccggc agatagcggt ctcggaacgg cgccctcgca
     1201 taatgcattt cgatggcgcg caggtgctga ctgcgccagg gcacggcctg attgatacag
     1261 acctcgctca ccagttgacc aaaacgatgg aggaccggca ccgtgatcca ctgacagcca
     1321 tccgatgttc tgatccgatt gcgattttgc cactcattct ttttgaactg cacggaatcc
     1381 aaggcaatga agagatccgc ctgatcgatc ttgtccagat aaccaagcca aggcagaaat
     1441 tgcggttgat gaatggtgac acgcatgagt cagcttcagc tctcaatctt tgagttgaac
     1501 ggtttctctg atgacatcga aggttccgag ctgaccgcgt tcggcccaag aatcggtccc
     1561 acaacaatcg gattactcag caactcaccg ctaccggtga tctccacccg ataagttagc
     1621 cgctctgctg tcggcacggt ggtgtcgggc cagtcgatga ggaatggtgt ctctcctgcc
     1681 aactgaacca gtacctgtcc ggatcgaatc acccgcactt tcacaggatg gcgtccacga
     1741 tcgctggccg tgacggctag gcgaatcacg aggtcgcgtt ccatgcccgg atccagccta
     1801 tcaccggccg aggcagattt gcttccaccc tggcatacga ttcgaaactc ttccagtcga
     1861 agttggacgt gatgatcgcc ttcgcccact gcataggaat gacccgattg aagcgcttcc
     1921 agtaccccgg caaccgtccg ttgtttcacc tggaccccca tcacaagacg gtgcagatcc
     1981 ttaccggcat cattcaatcc atgaaacgcc agttccccga tcaacacagg aatctgtcgc
     2041 tgctcctgtg caggcacttg tagcaaccgg tcccacagct cacccggctt gacgatggtt
     2101 cgtccatcct gatagagccc gccgaaacct gtgtagccgg tggtttgcac taacgcctcg
     2161 ggatgcggct ccgtcttaat gataaccgtt cccagtggtc cggcagcatg ctctcgaaaa
     2221 tcccgtgctt ccgtcatcga ccaaaaaacc agcccgccct tagccctcac atcatcgatg
     2281 agggcctgat agggtcgata cccaagccga tcgtcatacg aactaaacgc gggctggctt
     2341 agcggccaag aatagaccac cgatgccccc gctagaacga tcaggataca ggcggtagtc
     2401 cgtcgtaagg attgtcgatc ggatgcgcgc cggagcggcc tccagagcca cacggctgga
     2461 acagccaaca ggatcggcgc gccgttgatc acccctctcc aatcgaacgt ataggacccc
     2521 gggttcccac tcacagggag agagcgatag tcctccggct cggtcaggcc gaacaccaac
     2581 agattgcgtt gagcattgtg catcgtcaag gtgcgccccc atagagaacc ggtccaataa
     2641 taatagggag ccacctcgat gcccggcaca atgacgaggt ttggatggcg gatctgtgcg
     2701 gctgcgatct cctccacata tcgttcaatc ccatactcca gcacggatgg aaaacgaact
     2761 tgtcgcttca agagacctgg caagggccag agtccatact cataacggag cacgaagttg
     2821 tccgtcagaa tcagcacatt caaaccgagc tgttccgccc gttcggccag tgcttcaatg
     2881 gtcaagtcac cggtactggc agtactatgg acatgcaccg tggccagcaa ctgctgccga
     2941 tcatccgacg cctgcgcgtg cccaaccgcg cagcacaggc acaacagcac cgtcgcgaag
     3001 atcagtaccc cccaccccgc atctgtcatc cgcccgtatc gtctacagat gagcactgag
     3061 cgcctcggca aagatggatt cgatggcatg caccattgag gccgacccat atcggctgtc
     3121 tacgctatgg cttgcagcca cacctaactg cttggcccac tccggtcgat cgagcagccg
     3181 acgcaatgcc tctgccaacg catcggcatc acgaggaggc accagcaaac cagttcgctc
     3241 atggctgatc acggctggaa tgccgccgac gcgggaggca atcaccggca gtcctgctgc
     3301 catggcttcg atcagcgcac gccccatccc ttcattcagc gatggcagca cgaacaggtc
     3361 catccccgca agacaggctt cgacatcgtc cctgtgaccc aacagatgca ccgaatcatg
     3421 gaggccggcc gattccgctt gccgcgaaag tgcgtcatac ctgtctccac tcccgatgat
     3481 cacaagatgg agggaaggaa aatcctgctt caacttggca acagccgtaa tcaggtattc
     3541 atggccttta atgtcggtga gccacccgac ggatccgaca accagtgcat ccggtggaca
     3601 gccgaaccaa tccggctgtt gttttccctg agccctcgcc cgcccgaacc gctcacgatc
     3661 gatcccgctc ggcaccacgg cgaaccgatc ggctctgccg acagagcgat caaggtgatc
     3721 ctgccgttcc gcatccgtca gggcgatcag tcgatccgtg atcacactca gcacccgttc
     3781 gatctggaga aacagccatg attggaatgg accgaagtgg ccatagaaca catggccgtg
     3841 cggggtatgg acgatgacag gcacccgtgt aatccaggca gctattcgcc ccagcacacc
     3901 ggccttcgat gtatgggtat ggaccagtgc cggccgctcg tgccgaaaca ggcgaatcaa
     3961 ttgccacaga gcctgcgcat ccttgatcgg gtggagctct ctcgtcaacg aaggaagcag
     4021 cacccagcgg attgcggcct tctcaagttt ccggcaattt tcctcggtcg ccagctgccc
     4081 gccttgggca tcccaacagc caggatgccc agccaccact atcggctcga attgcgcacg
     4141 gtcatggccg agagcggtca gcatcgtgtt ctgcgccgac ccgccatggt caagtcgcgt
     4201 aattacatgg atcaccctcc ttgacttagg agccggtgag accataccgt tcgtcgaagc
     4261 agaagggcca ctgttggaca ggttcctgaa cgcctgatgc gaggctaccg gtctgtcctt
     4321 caataggttc atgaacagat gatccagatc agccgtatgc gccacccagt tgtagcgctg
     4381 ttcgacggcc tcgcggcctt tctttgacac acgctcccgc tcatgtggta tttcacgaaa
     4441 ccgattcagg accaatcgta gagtgttcgc aagagatact ccgtctgtcc cttccgtcac
     4501 cagcatcgga tcgaccctgc tcaacacctc tggaatcgcg ccgacgggcg tccccaccac
     4561 cggcgtgctg caggccagcg cctcgaccgt caccaacccg aatccttcga gttgcagcgt
     4621 cggcaacacg accaagtcag aggcctggta gtacttcgcc agctgttcct ccggcacgaa
     4681 tccgagcaac tgtaccgagt cttgtaatcc tctgctgcga atcaactcct ccaatcgcgg
     4741 acgcagttgg ccctcgccac cgatcaagag catgagattc tgttgctcct ctccgagcat
     4801 tgtcatggcc tcaagcagat attccaaccc catacggggt accaaattgc gaaccgtaaa
     4861 cattagcgtg cggccttcgg gcaacttcag ttcccgtctg atctcattcc tatcgtctac
     4921 cggatgaaac gtgaccggat cggcggctcc gggaaccacg gtaatatgct gcggcgcgat
     4981 tccatgcgca gccacgactt gtcgtttcat gaattcgctc aagacgatca catgatgaca
     5041 acggcggatc accgtgcgct caatccagcg gcgggcgcgc acgctcagtc gtcgcctaac
     5101 cccttccagt gtcgtcgccg cccgctccgc tctggtgaga tactcttcat gcgccaacga
     5161 atgacagaca tagacccagc gaccgaccat cccgtagcga aacagaatcg gaccgagacc
     5221 tgccaatgat tgatgcacta gggcgatatc gatcggagag gtctgccgca tgagatcaaa
     5281 tacccgaaga gagttccgca ccgaagaccg aacaaaagcc ggctcgctgg tccgcgtcac
     5341 agcataccgt cgttcggtca cggtcccaat tgtcagctcg gccgctgcag actcgaaggg
     5401 agcccgcgtc atgacgcgca cgtcatggcc cagcttggcg agtcccaatg cttgttgccg
     5461 gaggactcgc tcggcaccac cgatcacctg ttcgactgtg acttctgcaa ggaggacaat
     5521 gttcatgcca cg
//
LOCUS       DATIPF010000230         8076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_748918, whole genome shotgun sequence.
ACCESSION   DATIPF010000230 DATIPF010000000
VERSION     DATIPF010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748918"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VLL06_05865"
     CDS             <1..1015
                     /locus_tag="VLL06_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30526.1"
                     /translation="ELAHEAHVELQADEQAVLTRLGRIARALTELGLTDPEMGDCEQV
                     VTESTIQLKELAGRVRDYAQQLESDPDRQVVVEDRLDLIQRLKKKYGGTIEAVLAMGQ
                     GVQEELQLLDNHEARTAELTARLDEESRRLRTLAQQLSKKRIDAATRMTTLVEAELIA
                     LKMEQAIFQVTVASDESADGLGPIGRDRVEFLLSSNPGEPPRPLGRVASGGELSRIML
                     ALKTVLAEMDQVPVLVFDEIDTGVGGAVAAAMGTRLRKLGAFHQVFCITHLPQVASQA
                     EHHLLVEKGLEGQRTSTSVRVLKGIGREEEIARMLGGLTITKKVRETAAELIAGAQGK
                     RSE"
     gene            complement(1129..2268)
                     /locus_tag="VLL06_05870"
     CDS             complement(1129..2268)
                     /locus_tag="VLL06_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HSS30527.1"
                     /translation="MAPELTQKKPTLLVIDDEAGPRDALRVILRPFFNIQTAESASAA
                     INVLNSQTIDLITLDQKLPDRQGIELLQDIKQNHAGVEVIIITGYGSLRSAMEGIRHG
                     AAGYLLKPFNITELLSLVNQTLEKKQRLDFLRHFIETSTGLWGSEPECAHAWKELFSG
                     YRAIGKPASDKRSHTEDASDLLPMLSDLLEAKDRQLLNHCSRVSFYATLLANRINLTL
                     AEQKSLALGAFLHDIGKIGPEPYQFADDEIAVTGESFGNRHHPEQGAKLVLPLGLPTE
                     VGQIIAYHHERWDGSGYPFGLQEEGIPQLARIVCLAQTFDHLTAELPGRTPLSIDDAC
                     QQMLSYSGTHFEPKLTELFARVVQECKASLPAMAMATTPSDRSDS"
     gene            complement(2482..3897)
                     /locus_tag="VLL06_05875"
     CDS             complement(2482..3897)
                     /locus_tag="VLL06_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSS30528.1"
                     /translation="MKKRVLLIDDEARVRTSLKAVLEPTYETIQAADAQEGLELFRKE
                     APHLVLLDVILPGTDGLSVLQTMRAEGRTTPVIMLTGTKSVKTAVDAMKFGAADYLSK
                     PFDVEELRIIVERALNDQELQQEVKQLRAQVVRRYAFHNLIGKSPSMQDIYTKIEQVA
                     DSRTTVLIAGESGTGKELVAKALHYNSGRRERPFIALNCAALPETLIESELFGHEKGS
                     FTDATARRVGQFELANSGTLFLDEIGDLSAMTQAKLLRVLQEREFTRVGGVQSIKVDV
                     RIIAATNKNLEELVRKGHFREDLYYRINVIALYLPPLRERGEDVPLLAKHFLAKRIEE
                     DNRPPQEFAKDAVDLLSRYPWPGNVREMENIIEQAFIWSKGSETITSEHLPTILKNDT
                     RSSSLRDDTLAGRLSLEKAVMEFEREIILDALKRTNYVQTHAANLLGISRRMLKYRMD
                     TLGIGRTDQEVSTEPQAVVQE"
     gene            complement(3923..5200)
                     /locus_tag="VLL06_05880"
     CDS             complement(3923..5200)
                     /locus_tag="VLL06_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS30529.1"
                     /translation="MTSRPSNTNILAPSSDELLNIVTRFAADLGSMTDLMTLGTRIME
                     ELCRAGSTTHGALFLLDREHGYYRRTSTVGPVAPMLIPQTLVASHPLPRHLLATNRIL
                     TQIDLATDTQETDSGAGARAAMEAMHATRIVPHVNKGYLIAFSLLGTGEPLVEENALT
                     RSVLATLAQTATNALDTMVLYEDLHRSHTLIKRTDRLKSLETMAGGFAHEIRNPLTSI
                     KTFIQLAPERKDDPQFIQEFSKVVLDDVYRIERLIQEILDYARYMEPKLTDEDFNDIV
                     ASCLYFIDVKADNHGIKIEKELAPDLPRVMLDRQQIKQVLLNLLLNAMASMATSGGRL
                     RVQTRKLLKPGGKIWAHIEIEDTGEGIQDTNLEHIFDPFFTTKHESGEHEGTGLGLTI
                     VHQIIQEHHGEILVKSSVGVGTTFFVSLPALPA"
     gene            complement(5324..>8076)
                     /locus_tag="VLL06_05885"
     CDS             complement(5324..>8076)
                     /locus_tag="VLL06_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS30530.1"
                     /translation="LVRQLGGTLDVSVPMQGPLRIILRLPVASQLPLEALQKQDSVSP
                     GPPTLAPETKSAETVSVSAADSGRPQQERRHSPRIPTTLPATITVGATTWEGTISNLS
                     LGGTCITLPSDFPAVTPQDAYVILKTAVGILELHGEAQERPIWLPLGTPVSHLVIAFD
                     LPKREEAAVLASLIQAAQEQTVPFGLEVLLAAEPQVQLSATAPLVSSRGADYDLREAV
                     RVTVTLPVRLDLMDHTGRPHQLAALTINFSRDGVCLLLNARPELLSGLATLRFATTQT
                     QSHPGTHEPGAPDSALPARIIWSAPDPATPSEYRHQSSDPVIRVGLRFWGLTPYAERE
                     VTRVVRQHLTSPSESEQTSQQATVASIPRECRNPRGQAIMITDDHLSQSLAPNTPVVI
                     IAPGYGQTALDAITSSYYLAHHRLRVLRYDHTNHVGLSDGELQQTTLRSMQADLLKVV
                     EFVQHTWPTAPLIIMASDMTARVALKVAVQSRPLDLLLLINPVVDVQAMLMSVHGHDL
                     VADHRYGLRRGIANLLGLNINVDRFVGDIVAGHFTDLASTIADLRLLRSPSAILTIPS
                     NPLGPLPPADLPQTFLTALGTHTRLATVPAPLFGQDLPLNDQHPPAFRQLLDQISATV
                     ALPTIPAEFRTHSRRALARQQRIETERTRLRHNLSQIAREVLRVAHLQLPQLGNLHEH
                     WKLLDDLYRLLSPLEPGTMLVDVGVGHGDLVRATMVNQAYRSRQRGWSPDRPVQVIGL
                     EYSQDSLTQARQSLRALHRELDSDFAGTLTIHPPLTAEWVHTDWTQGLPFKDHSLYRI
                     VCNLSLPFVPSPLVTIRELYRVLHPQGRLVLTVFHPNTDLSVLYRRHLHRANQDEFSP
                     QAQIVLHYLGRLREAIRHGLLHTFDRSSLASFLQQAGILTPRILSALDGHALLAVIEK
                     GK"
BASE COUNT         1732 a         1983 c         2545 g         1816 t
ORIGIN      
        1 ggaactggcc cacgaagcgc atgttgagtt acaggcggat gaacaggcgg ttcttaccag
       61 actggggcgg atcgcgcgag cccttacaga gttgggcctg actgatccgg agatgggtga
      121 ctgtgaacag gtggtcacgg agtcgacaat ccaactgaaa gaactggcag ggcgggtgcg
      181 cgactatgct caacagttag agtccgatcc tgaccggcag gttgttgtgg aggaccgact
      241 cgatctcatc caacggctga agaaaaagta cggaggaacg atcgaggcgg tattggcgat
      301 gggtcaaggg gtgcaggagg aacttcaact attggacaat catgaagcga gaactgccga
      361 gttgaccgcg cggttggacg aagagtcccg ccgtctccgc acattggcac agcagttgtc
      421 caagaagcga atcgacgcgg ctacgcgaat gacgacgctc gtcgaagctg agctaattgc
      481 gttaaaaatg gagcaggcga tctttcaggt cacggtggcc agcgatgagt cggcagatgg
      541 ccttggaccg attgggcgcg atcgagtgga gttccttctc tcaagcaatc cgggtgaacc
      601 accgagacca ctgggccggg tggcatccgg tggggaactt tcgcgtatca tgctggcgct
      661 caagacggtg ttggctgaga tggaccaggt tccagtcctg gtatttgacg aaatcgatac
      721 gggagtcggg ggggcggttg cggcagcgat gggcacgaga ctacgcaagc tcggagcgtt
      781 ccatcaagtc ttttgcatca cccaccttcc tcaggtcgcc tcccaggcgg aacatcatct
      841 gctggtagaa aaggggctgg aggggcaacg gacatccacc tctgtcagag tgctcaaggg
      901 gattgggcga gaagaggaaa tcgcccgcat gctgggcggc ctgaccatta cgaagaaagt
      961 gcgtgaaacg gcagccgaac ttatcgcagg ggcgcagggg aagcggtccg agtgagctga
     1021 ttggtcttgt gtatccggtg atgtggtgag tctggttcaa ccaaagaaac aagacagacc
     1081 acacaagcca attgaacgaa acaggccggc ttgcgttccc agctgctgtt aggagtctga
     1141 tcggtcgctt ggagtggtcg ccatcgccat ggcagggagc gatgccttgc attcctggac
     1201 cactcgcgca aagagttcag tcaacttcgg ttcgaagtgg gtgccggaat acgagagcat
     1261 ctgttggcag gcgtcatcaa ttgaaagggg cgtccggcca ggaagttccg cagtcagatg
     1321 gtcgaacgtt tgggccagac agacaatgcg agccaattga ggaattccct cctcctgaag
     1381 tccaaatgga tagcctgaac catcccaccg ttcgtggtga taggcaatga tctgtcccac
     1441 ctcagtcgga agtcccagcg gcaagacgag tttcgcaccc tgctccgggt ggtgccgatt
     1501 gccgaaagac tctccagtca cagcgatttc atcgtcggca aactgatagg gctcgggccc
     1561 gatcttgccg atgtcgtgga ggaaagcccc cagcgccaac gatttttgtt cagcaagtgt
     1621 gagattgatg cggttagcta aaagcgtggc atagaaactc actcggctgc agtgattgag
     1681 caactggcga tctttggctt caagcaggtc ggagagcatg gggagtaggt cggacgcgtc
     1741 ttcagtgtgg gagcgtttat ctgacgcagg ttttccgatt gcccgatatc ccgagaataa
     1801 ctccttccat gcgtgagcac attctggttc tgatccccag agccccgtcg atgtttcaat
     1861 aaaatgtctg aggaaatcga gccgttgttt cttttcgagt gtctggttca cgagggaaag
     1921 cagttctgtg atgttgaagg gtttgagcag gtagccggca gcgccatgcc gtatcccttc
     1981 catggcggac cttaagctcc catatcctgt gataataatg acctcaacgc cggcgtggtt
     2041 ctgcttgatg tcctgaagga gttcgatgcc ctggcgatct ggaagctttt gatcgagtgt
     2101 gatgaggtcg atcgtctggg agttcagtac gttaatggct gcactggctg attccgcagt
     2161 ctggatattg aaaaatgggc ggagaatcac tcgcagagca tcacgaggtc ctgcttcgtc
     2221 gtcaatcacg agcagggtcg gtttcttctg agtcagttca ggagccataa tgacgttcat
     2281 cagaatattc gctaccgtca actacgtatg atcgacgcaa ttttcattcc atttcagtga
     2341 gttagcacag tatctcaaca gagtgtaggt tgatgacgac cgagccccat atgtagattt
     2401 cttgagcgaa acactacgta catcagcagg gaaacgtcat actcagttgt gcaaaaaaga
     2461 cgcagtaggg gcattgttgt gctattcctg cactacggcc tggggctcgg tgctgacttc
     2521 ctgatccgtt cgaccgattc ccagtgtgtc catgcggtat ttgagcatcc gacggctaat
     2581 tcccagcaga ttggcggcat gggtttggac atagttcgtt cgcttgagtg catccaagat
     2641 aatttctcgc tcaaattcca tcacggcttt ttcgagcgag agccgtccag caagcgtatc
     2701 gtcacggagg gaagaggatc tggtgtcgtt cttgagaatg gtcgggagat gttcggaggt
     2761 gatggtctcc gatcctttgg accaaatgaa tgcctgctcg atgatatttt ccatctcacg
     2821 cacattgcct ggccagggat agcggctcaa gaggtcgacg gcatctttgg cgaactcttg
     2881 gggcggtcga ttgtcttctt cgattcgttt cgcgaggaaa tgtttggcga ggaggggaac
     2941 gtcttcacca cgttcccgga gcggaggcag atacagtgca atgacgttga tgcggtagta
     3001 gaggtcctcg cggaagtgcc ctttgcgaac gagctcctca agattcttgt tcgttgccgc
     3061 gatgatgcgc acatccacct tgatggattg caccccgccg actctggtga actctcgttc
     3121 ttgtaagacg cgcaggagct tggcttgcgt catggcgctg aggtcgccga tctcgtcgag
     3181 gaagagcgtg ccactgtttg ccagttcgaa ttgtccgact cgacgggccg tggcatccgt
     3241 aaatgagcct ttctcatggc cgaacagctc actctcgatg agagtttcag gaagagcggc
     3301 gcagtttagc gcgatgaagg gacgctcgcg cctgccgcta ttatagtgga gcgccttggc
     3361 gacgagttcc ttccccgtgc cgctctcgcc ggcgatcagg acggttgtgc ggctatcggc
     3421 gacttgttcg atcttggtat agatatcttg catcgacggg ctcttgccga tcaggttgtg
     3481 aaaggcataa cgccgaacga cttgcgcacg gagttgtttg acctcttgtt gcagctcctg
     3541 gtcattgagc gcgcgctcga cgataatgcg tagttcttcc acatcgaagg gttttgaaag
     3601 atagtcggcc gcgccgaatt tcatcgcatc gacggcggtt ttgactgatt tcgtccccgt
     3661 caacatgatc accggtgtcg tacggccttc ggcgcgcata gtttggagaa ccgacagccc
     3721 gtccgtgccc ggcagaatga cgtccagaag gacgaggtgg ggggcttctt tccgaaagag
     3781 ctcaaggcct tcctgcgcat cggcagcttg aatcgtctcg tatgtcggct ccagcaccgc
     3841 tttcagtgag gtcctcaccc gggcctcatc atcgatcaac agcactcgtt ttttcatact
     3901 ggtgtccata gggtgttggc ggctatgcag gtaacgcggg taagctcacg aagaacgtgg
     3961 tgccgacacc gaccgagctc ttcaccagga tctcgccatg atgttcttga ataatttgat
     4021 ggacgatcgt caagccaagc cccgttcctt cgtgttctcc gctctcgtgt ttcgtcgtaa
     4081 aaaacgggtc gaatatatgt tcaagattcg tatcctggat tccttcgccg gtatcttcga
     4141 tctcaatgtg ggcccagatt tttcctcccg gcttcagcag cttacgggtc tggacgcgta
     4201 agcgtccccc cgatgttgcc atcgaggcca tggcattgag gagaagattg agcaggacct
     4261 gcttgatctg ctgccggtcc agcatgacac gaggcaggtc aggcgccaac tctttttcaa
     4321 tcttaatccc atggttgtct gctttgacgt cgatgaaata caagcacgac gcgacaatat
     4381 cgttgaagtc ctcatccgtc agcttaggct ccatatatcg ggcatagtcg agaatttcct
     4441 ggatgagccg ttcgatacga tacacgtcgt cgagcaccac tttgctgaat tcttgaatga
     4501 actgaggatc gtcttttcgc tcaggggcca attggataaa cgtcttgatc gacgtgaggg
     4561 ggtttctaat ttcatgggcg aacccgcccg ccattgtttc cagagatttt agccgatccg
     4621 tgcgtttgat aagcgtgtgt gagcgatgga ggtcctcata cagcaccatg gtgtctaacg
     4681 catttgtggc ggtttgtgcc agggtggcga gcacggatct ggtgagtgca ttttcttcaa
     4741 ccagaggttc ccctgtgcca aggagtgaaa acgcgatgag atacccttta ttgacatgtg
     4801 gcacaatccg tgtggcatgc atggcttcca tggctgcgcg tgcgccggct ccggagtcag
     4861 tctcttgtgt gtctgttgcc aagtcgatct gcgtcaggat ccggttggtt gcgagtaggt
     4921 ggcggggcag aggatggctc gccaccagcg tctgaggaat cagcatggga gcgactggac
     4981 caacagtgct ggtacggcga tagtatccgt gctcgcggtc caggaggaac agtgctccat
     5041 gggtcgtgga tcctgcgcga cacagctcct ccataatacg ggtgccgagt gtcatgaggt
     5101 cggtcatcga tcccaggtcg gcggcaaacc gcgtaacgat attcaataac tcatcggagg
     5161 aaggcgccaa gatattcgta ttggatggac gtgatgtcat aacgaacaga gtgcgcaagg
     5221 acgccgtgcg atccaccaga gaaaatacat atggtgcgtg aaacggtatg tgttcaaaga
     5281 tgtacgggtg ggagtataca cgggcgggac acgttcagct agctcattta cctttttcga
     5341 tgacggctaa aagggcatgc ccatcaaggg cggaaaggat tcggggtgtt aggatgccag
     5401 cttgctggag gaacgaggcc agtgaggacc ggtcgaaggt atggagcaat ccatgccgaa
     5461 tggcttcacg aaggcgcccc aggtagtgca ggacgatttg ggcctgcgga ctgaattcat
     5521 cctgattcgc tctgtgcaga tgccggcgat aaagcactga gaggtcggtg ttggggtgga
     5581 aaaccgtgag gactaagcgc ccctgtggat ggagtacgcg gtacagttcc cgaatcgtaa
     5641 ccagtggtga ggggacaaac ggaagggaca ggttgcagac gatgcgatag agggaatggt
     5701 ccttgaacgg aaggccctgc gtccaatccg tatggaccca ttcggcggtg aggggtgggt
     5761 ggatagtgag ggtaccagca aagtcactgt caagttcccg gtgcaaggca cgaagactct
     5821 gccgagcctg cgtcagggaa tcttgagaat attccaggcc gattacctga acgggacggt
     5881 ccggactcca tccccgctgt cgcgagcgat aggcttggtt gaccatcgtg gctcggacta
     5941 aatcgccatg gccgacaccc acatccacga gcatggtgcc tggttcgagc gggctgagta
     6001 gtcgatagag atcatccagg agcttccagt gttcatggag gtttccgagt tgggggagtt
     6061 gcagatgcgc gacccgtaag acctcgcgcg cgatctgcga aaggttatgg cgaagacgag
     6121 tgcgctccgt ctcgatgcgc tgttgacggg ccagtgcgcg acggctgtgt gtccggaatt
     6181 cagctggaat agtcggcagt gcaacggttg cggagatctg atccaggagt tgtcggaatg
     6241 ccgggggatg ttgatcattg agcggaagat cctggccgaa cagtggtgcg gggaccgttg
     6301 cgagacgggt atgggtgccc agcgcggtga gaaaggtctg tggaagatca gctggaggca
     6361 acggcccgag cgggttgctg ggaattgtga gaatggctga cggagagcga agaagacgca
     6421 ggtctgcgat cgtggaggcc agatctgtaa aatgtcctgc gacgatatcg ccgacaaagc
     6481 gatcgacatt gatattgagt ccgagcagat tggcgatgcc gcgtcggagt ccataacggt
     6541 ggtcggccac gaggtcatga ccatggacag acatcagcat cgcctgaaca tcaacgaccg
     6601 gattgatcag gagaagcagg tcgagggggc gggattgtac cgccactttg agcgcgacac
     6661 gtgcggtcat gtcgcttgcc atgatgatca gaggcgcggt tggccacgta tgttgcacaa
     6721 actctacaac ttttaggaga tcggcttgca tgcttcgcaa ggtcgtctgt tgcagttcgc
     6781 cgtcgctcaa tcccacgtga ttggtgtgat cataacgtaa gacacggagc cgatggtgcg
     6841 ccaggtagta tgacgaggtt atggcatcca acgcggtctg gccatagccg ggagcaatga
     6901 tcacgaccgg tgtgttcggt gcaagcgact gagaaaggtg gtcgtcagtg atcataatcg
     6961 cctggccacg cggattccgg cactcacgtg gaatactggc gacggtcgcc tgctgcgagg
     7021 tctgttctga ttcactcgga gatgtcagat gttgtcgaac gacacgtgtc acttcgcgct
     7081 cagcataggg tgtaagaccc caaaagcgga gcccgacccg aatgactggg tcggagcttt
     7141 ggtggcgata ttcgctgggt gttgcagggt ctggagcaga ccagatgatc cgtgcaggca
     7201 gcgcggaatc cggtgcgccc ggttcatggg tcccggggtg gctctgggtc tgtgtggtgg
     7261 cgaagcgtag tgtcgcgagg ccgctcagga gttcggggcg agcgttgaga agaagacaga
     7321 caccgtcccg gctgaaattg atcgtcaggg ccgccagttg gtgagggcga ccggtatggt
     7381 ccatcaggtc gagtctgacc ggaagtgtca ctgtcacccg aactgcctcg cgcaggtcgt
     7441 agtctgcccc tctgcttgat acaagggggg cagtagcact cagttggacc tgtggttctg
     7501 ctgcaagtaa gacctcaaga ccgaaaggca cagtctgttc ctgtgcagct tggatcaatg
     7561 aggccaggac ggcggcctct tctcgcttgg gcagatcgaa cgcgatgacg agatgggaga
     7621 ccggtgttcc gagagggagc cagatggggc gttcctgtgc ctcaccgtgt agttcgagta
     7681 tccccacggc agttttgagg atgacatagg catcttgtgg tgtcacagcg gggaagtcgc
     7741 tggggagtgt gatgcaagtg ccgccaaggc tgaggttgga aatggtgccc tcccacgtcg
     7801 ttgccccgac ggtaattgtg gcaggtaggg ttgttggaat tcgaggagaa tggcgtcgtt
     7861 cctgttgcgg tctgccagag tcggctgccg aaacagatac cgtctcagca ctctttgttt
     7921 caggggcgag tgtgggcggg ccgggagaga cggagtcctg tttctggagc gcttcgaggg
     7981 ggagctggga ggcgactggc agacggagaa tgatacgaag agggccctgc atcggtactg
     8041 acacatccaa cgtgccaccg agttggcgaa caagcg
//
LOCUS       DATIPF010000231         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1720822, whole genome shotgun sequence.
ACCESSION   DATIPF010000231 DATIPF010000000
VERSION     DATIPF010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1720822"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1482..>2212
                     /locus_tag="VLL06_05890"
     CDS             1482..>2212
                     /locus_tag="VLL06_05890"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HSS30531.1"
                     /translation="MALRGISQPMPSMDPQTGLVECVWRDPYILLVGEADQEDWVSGI
                     YLVKLTASGSGAQAYIIFVVRDDTRPSDWLFQSSVTTYQAYNNWGGKSLYDFNSREGR
                     AYKVSFNRPYAISSNPAAASGNGAGDFLTNNAVPPADASSASGWEYNMVRWLEREGYD
                     VTYSTNVDTHSNPRLLDRHKGWLSVGHDEYWTWQMRANVECARDCGLNLAFFSGNVCY
                     WQIRLEPNLSTNEPDRTIVSYKDDAP"
BASE COUNT          565 a          567 c          605 g          475 t
ORIGIN      
        1 gcggcctcac gatgagcgag acggccacgg cgagcccggc gaccagcagc agcgcgaccg
       61 cggtcgcgtg gccggacggg ccgaggcgcg acagcaggtc accaggcgtc atggacaccg
      121 ccggggctca gctataaatt cgtacgtaaa cttaaagaca gcaactggtg tgcctgtttc
      181 gtctcatcac ataattccac acatgcacat gtgaaatacc ctgatcgcat ggatggaaag
      241 caggacatca catcatcgaa aatggttacg aaaatatcgc ggattcggtc acggccatta
      301 tagataaaga aaaatgaagg gaggatgaag caatcatgaa gaaatgatca tgaaggcgcg
      361 tcctatactt actcccattt ctggctcatc aacaatgaac tcgggaagtc cacagggccc
      421 gtcattctcg cggaagcata caataccact gtcggcgtct cgtatgcttt cgtactgtgt
      481 ttaggtctgt cgaatgtaac gggagcagcc agaggaggtt cggtcgttac cgacaccatc
      541 gagggcaaag taataagcgt ggaaggcgat gattatttga ttcaactttt gacgggaggg
      601 aagtgcggtt gcatgctgat gcaaacactc aacggatggg accccattag gcaagtgatc
      661 gtattgaagc caaagtgaac gaaacgaatc aggagcaggc attgtctatt cgcccgacga
      721 aataaagaga gtcaagcgga agtgaaacgc tgaattgtcg ccgtcgagct tctctaagcc
      781 gctccgtcct ttgttagctg gcggaagcgt gtgcatgata aatatgtgtc cttcttggtc
      841 ctcgtgaacg agcatcacat cctagggaac tatcggcttc gcgaggagac cagcttgctg
      901 gagcggatgc tacagattag cctgacaact gggcacttga ctgtatggtg gcgtaaacca
      961 aaaacgatca atcacaaaac tgacggggcc actggggagg tccgatccgc agactcaaaa
     1021 gtccaacaga gatcacagaa ttacctcgta gacccagcaa tctagttgat cccaattaaa
     1081 tgtactcgta ctcccatatg tattaccaat ggtcaaatta attgaaattg gatgttggca
     1141 agagattggg ctgttgcgat cgtgcaaggt gtcggtgaag gcgtcgacga tcagttgtct
     1201 cctctcactc tccgcacacc aacgccacct cgttcgtgcc tcgggtagcg aggtcagaaa
     1261 ttaatccaat cgaacgagag aatgcacgac tcggtacatt cgactggcgg gtcaccaacc
     1321 ccgccctgca tcgcgagatt gaaggctatg cttcactcac aagcgtgaat cgaagccacc
     1381 agatccggtt ctacatccat acgcaggact cctcatatag cttggcgatt taccgaacag
     1441 gatggtatgg gggcgttggt gctagactcg tgcatggtcc tgtggctctc cgtggaatat
     1501 cgcaaccgat gccgtcaatg gatcctcaga ccggactcgt ggaatgtgtc tggcgggacc
     1561 cttacatcct cttggtcggt gaggccgatc aggaggactg ggtcagcgga atctatctcg
     1621 tcaaattgac ggcgagcggg agcggtgcac aggcttatat catttttgtc gtgcgcgacg
     1681 acacgcgccc atccgactgg ctcttccaat ccagcgtgac gacgtaccag gcctacaata
     1741 attggggcgg aaaatcactc tatgatttca atagtaggga agggcgagcc tacaaagtct
     1801 ccttcaaccg accctatgca atcagcagca atcctgctgc cgcttctggc aatggcgcag
     1861 gagactttct gaccaacaac gcggtgccgc cagcagatgc gtcctcagcc tcggggtggg
     1921 aatacaatat ggtgcgatgg ctcgaacggg aagggtacga tgttacctat agcacgaacg
     1981 tcgatactca ttcgaatccc cggctcctag accggcacaa agggtggctt tccgttggac
     2041 acgacgaata ttggacgtgg cagatgcggg cgaatgtgga atgtgcacgc gactgcgggc
     2101 tgaacctcgc ttttttctcg ggtaatgtct gctactggca gatccgctta gagccgaacc
     2161 tttccacgaa cgagccagat cgcacaatcg tctcgtacaa ggacgatgca cc
//
LOCUS       DATIPF010000232         4364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_217702, whole genome shotgun sequence.
ACCESSION   DATIPF010000232 DATIPF010000000
VERSION     DATIPF010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217702"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VLL06_05895"
     CDS             <1..383
                     /locus_tag="VLL06_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30532.1"
                     /translation="DLDTGSVVGQSFKELDVDAQLFAVEIGEIKTWYPPVTIIDFKVR
                     PGRPVLLKVTNKATAEHGFEMTDATNAGAPTVMKAQVVLKPGETKYIGIPTSDMFYAT
                     QGNTLTYRCHLHPGHVGGKILMIK"
     gene            complement(534..881)
                     /locus_tag="VLL06_05900"
     CDS             complement(534..881)
                     /locus_tag="VLL06_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSS30533.1"
                     /translation="MALLITDECISCGACLPECPNEAIFETRSDAETKGHHVGDGQGV
                     GDSIYVIAHDRCTECVGHFDEPQCAAVCPVDNCCISDPLYPETTEVLLERAKTLNPDK
                     AIDAAKVWSGVRN"
     gene            1083..1592
                     /locus_tag="VLL06_05905"
     CDS             1083..1592
                     /locus_tag="VLL06_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HSS30534.1"
                     /translation="MRKYPMATVSVSDGGQKKKRIITLVLMAMFLMSASFFLIDRKDE
                     TIIIVSFPNGRELETEVADTPEKLLVGLAFREALPANGGLLYIFESTGQNHVWTKEYR
                     FPVDMMWVDESHHIVGLKENIAPCREADCPKYSSSPEAVRYAIQTAAGFIKQEGVTIG
                     QELKYTLRM"
     gene            1650..1970
                     /locus_tag="VLL06_05910"
     CDS             1650..1970
                     /locus_tag="VLL06_05910"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HSS30535.1"
                     /translation="MLEGFQSVALVNELPPGQAKAVMVNNREIALFNIEGKYFAIHNI
                     CPHEGGPLCKGRVKGFVVACPWHDLAFDVRNGQGTDGGGYCVGSYHVQVEGEEIFVGP
                     RRKP"
     gene            2027..2377
                     /locus_tag="VLL06_05915"
     CDS             2027..2377
                     /locus_tag="VLL06_05915"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HSS30536.1"
                     /translation="MSGHDHHDSATRSDGAKVIETTLDHPIQIHLWEDRTRGELWVPT
                     FDPTGLELLADDYLRIAGNNAVDNGQRTFEFKAVRAGTHRVVFEKRMGWKFTAEDRRV
                     FDVTVSDASSRRSA"
     gene            2380..3219
                     /gene="dat"
                     /locus_tag="VLL06_05920"
     CDS             2380..3219
                     /gene="dat"
                     /locus_tag="VLL06_05920"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR01121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047810 - D-alanine:2-oxoglutarate
                     aminotransferase activity [Evidence IEA];
                     GO_process: GO:0019480 - L-alanine oxidation to pyruvate
                     via D-alanine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HSS30537.1"
                     /translation="MPNVAFINGAFVPLADAKVSIEDRGFQFGDGVYEVIRTYKGRPF
                     ALEAHLARLDHSATALDLTQPYSHAEWTDHILEGVRRAAFPEAKIYIQLSRGVAPRDH
                     AYSTELTPTVVMTVREFHPLDRSVQAAGVDALTTEDIRWGRCDIKSVNLLANVLARQQ
                     VKQAQVFEAILVRAGLVTEGAVSTVMVVQGGTVMTAPEGPHILSGVTRAVVLDLARSE
                     GIAIQERCVSQEDLYGADEVFLTGTTVEVLAVVRIDGKIIGDGRPGPITQQLAAGFTK
                     QVG"
     gene            3299..3814
                     /gene="rimP"
                     /locus_tag="VLL06_05925"
     CDS             3299..3814
                     /gene="rimP"
                     /locus_tag="VLL06_05925"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HSS30538.1"
                     /translation="MNEPSKDTGLGVSVKESRPVVERISEVVSPILWALGLELVDVVC
                     VGQGARSIVRVYIDKSGGVTVDDCGRAHLAIGPALDVADPFPHAYTLEVSSPGLDRAF
                     KRIQDYRRALGKQVSVKLRQPIDGQWRMVGVLTEVNEQGVTITVNDPRSERKTSLEFG
                     SIAEARRAVTF"
     gene            3895..>4364
                     /locus_tag="VLL06_05930"
     CDS             3895..>4364
                     /locus_tag="VLL06_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HSS30539.1"
                     /translation="MNRELISVIDELGRQKGIEKSRVIGAIEAALQMAAKKRFGQAEN
                     IQVEIDSKTGEISVVSKKTIVDAVVNPKTEVSLQEARQFDSEAEVGDEIGSLIEMSDL
                     GRIAAQTAKQVIFQKVREAEWEAVQKEYSTRQGDLVNGIILGMERRNYLVDLGKT"
BASE COUNT          997 a         1063 c         1326 g          978 t
ORIGIN      
        1 gggacttgga tactggctcc gtcgtaggtc agtcgttcaa ggaattggat gttgacgccc
       61 agctgttcgc agtcgagatt ggggagataa agacttggta tccgccagtc acaatcatcg
      121 atttcaaagt tcgacctgga cgtcccgtcc tgctcaaggt cacgaacaaa gccactgctg
      181 agcatggatt tgagatgacc gacgcgacaa atgccggagc cccgaccgtc atgaaggccc
      241 aagttgtgtt gaagccggga gaaactaagt atatcggtat cccaaccagc gacatgttct
      301 acgccacgca gggaaatacg ctaacctatc gctgtcacct gcacccgggg catgtcggcg
      361 gtaagattct tatgatcaaa tagtcgttct tgacagatgt gagtgaccat cgccctcttt
      421 gcgataccgc ggagagggcg atggtttttt agcaggatgc tagaggcagg aaggccctgc
      481 ttctcttatg agaagcaggg ccttccttat taagcaatga gcggatgaga tgattagttg
      541 cgtaccccac tccagacttt cgccgcatcg atggccttat ctggattgag tgtctttgcc
      601 ctctcgagca ggacctccgt tgtttccgga taaagcgggt cagaaataca acaattatcg
      661 accgggcaga cggcggcgca ctgcggctcg tcgaagtgtc caacacactc ggtgcaacgg
      721 tcgtgtgcaa tcacataaat actatcgcca actccctggc catcgcccac atggtggccc
      781 ttcgtttccg cgtcgctgcg agtctcaaaa atcgcctcgt tcggacattc tggcaggcag
      841 gccccacagg agatacattc atcggtaatc agcaacgcca tgaccctcgc ctccttctac
      901 acgacaatag agacacacac ggttgacaag ttcaccccgt cacgaccatt atgcgtggcg
      961 cgatgtgacc gccattctag tccgccgctc ttttccaagt caacagaacg gcacttgctc
     1021 agaaggccta gattccctgt attactgggc ctttgggctc agggcatcgt gacgagttat
     1081 ggttgagaaa atatcctatg gccactgtgt cggtgagcga tggagggcag aaaaagaagc
     1141 gcatcatcac gcttgtcctc atggcgatgt ttttgatgag cgcgtcgttc tttctcatcg
     1201 atcggaagga cgagaccatc attatcgtgt ccttccccaa tggccgtgag cttgagacgg
     1261 aagtggcgga tacaccggag aaactgctcg tcggtcttgc atttcgagag gcgttgccgg
     1321 caaatggtgg attgctttat atctttgagt cgaccggcca gaatcacgtc tggacaaagg
     1381 agtatcggtt tccggtggac atgatgtggg ttgatgaaag ccatcatatt gtgggactca
     1441 aggaaaatat cgctccttgc cgggaagctg actgccccaa atatagttcc tctcccgaag
     1501 cggtccgcta tgcgattcaa actgcagcag gttttatcaa gcaggagggg gttaccatag
     1561 ggcaggagct gaaatatacg cttcggatgt aacacgcgcg ggaggcgtgg cacagcgcat
     1621 gaagacatga agaaaggagt ggggagagca tgctagaggg gtttcaatcc gtcgccctgg
     1681 ttaacgagtt gccgcctggg caagcaaaag ccgttatggt gaacaatcga gagattgcac
     1741 tcttcaacat cgaggggaag tattttgcga ttcataatat ttgtccacat gaaggcggcc
     1801 ctttgtgtaa agggcgtgtg aagggctttg ttgtagcctg tccgtggcac gacttggcat
     1861 tcgacgttcg aaacgggcaa gggacggacg gcggtggata ttgtgtggga agttatcatg
     1921 tgcaggtgga aggcgaggag atttttgtcg ggcctcggcg caaaccatag cagaatcaga
     1981 caattaattt agggggcggg ttgccgcata catgacgatg agtgcaatgt ctggccatga
     2041 ccatcatgat agtgccacac gctcggatgg agccaaggtc attgagacaa cacttgacca
     2101 tcccatccag attcatctct gggaagacag aacgcgggga gagctctggg ttccgacctt
     2161 tgatcctacg gggctcgagt tgctggcgga tgactaccta cgcatcgccg ggaataatgc
     2221 ggtcgataat gggcagcgga catttgagtt taaagcggtc agggcgggaa cccatcgggt
     2281 cgtgtttgaa aaacgcatgg gttggaaatt caccgccgaa gatcggcgcg tctttgacgt
     2341 gacggtctct gacgcctcgt ctcggaggag tgcgtaatcg tgccgaatgt ggccttcatc
     2401 aatggagcgt tcgtgcctct ggccgacgcg aaagtctcga tcgaagatcg aggctttcag
     2461 tttggcgatg gcgtctacga agtgatccgt acctacaagg ggcgtccgtt cgcgctcgaa
     2521 gcccatctcg ctcggctcga tcacagcgcg acggcacttg acctgacaca gccctattcc
     2581 cacgccgagt ggacggacca tatcctggaa ggagtcagac gggctgcctt tcctgaagcg
     2641 aagatctaca ttcagctcag caggggtgtc gctccccgtg atcacgccta ttcgaccgaa
     2701 ctcacaccaa cagtcgttat gacagtacgt gagttccatc cgctcgacag gtcggtgcag
     2761 gcggccggtg tggacgcgtt gacgacagag gatatccgat ggggacgttg cgacatcaag
     2821 agcgtcaatc tcttggccaa tgttcttgcg cgacaacaag tcaagcaggc ccaggtcttc
     2881 gaagcgattt tagtcagagc gggattggtc actgaagggg cggtcagtac tgtcatggtg
     2941 gtccaggggg gaacagtgat gacggctcct gaagggccgc acattttgtc gggggtgacg
     3001 agggccgtcg tgttggattt ggctcggagc gagggtatag cgatccagga acgctgtgtc
     3061 tcgcaggaag atctttatgg ggctgacgaa gtttttctta cagggaccac ggtcgaagtc
     3121 ttggctgtgg tccgtatcga tgggaaaatt attggagatg gacggcccgg cccgattacc
     3181 caacaactcg ccgctggctt cacgaaacag gtcggctagc ccccttgctt tggcataggt
     3241 agcatgctat ggtcctcggg ctggaagtgg gctcaggccc acttttttgt ttgatctcgt
     3301 gaatgagcca tccaaagata cggggctagg ggtgtcggtg aaggagtccc gaccggttgt
     3361 cgaacgcatc agcgaggtgg tgtcgcccat cctctgggcg ttgggcttgg aattagtcga
     3421 tgtcgtgtgc gtggggcaag gggcgcggtc aatcgtccgc gtgtatatcg ataagtcggg
     3481 cggcgtcacg gtagatgact gcgggcgggc acacctcgcg attggtccag ccctggatgt
     3541 ggctgatcct ttccctcatg cctatacatt ggaagtctcc tcgccgggtc tggatcgggc
     3601 cttcaaacgg attcaggatt atcgccgggc cttggggaag caagtgagcg taaagctcag
     3661 gcagccgatc gacggacagt ggcgtatggt cggagtgttg acggaagtga atgaacaggg
     3721 agtcacaatt acagtgaatg atccgcgttc tgagcggaag accagccttg agttcggctc
     3781 aattgctgag gcgcggcgcg ccgtcacgtt ttaagcgtga ccgaaatcga ttaccgcggt
     3841 gcagtaatcg attattgtcg ggttgttgca ggggcagttg ttggagagtg agctatgaat
     3901 cgagaattga tttcagtgat cgatgagctt ggtcggcaaa agggcatcga gaaaagccga
     3961 gtaatcgggg cgatcgaagc cgcccttcaa atggcggcga agaagcggtt cgggcaggct
     4021 gagaatattc aagtcgagat cgactccaag accggtgaga tttccgtcgt gtcaaagaag
     4081 accatcgttg atgcggtcgt caatccaaaa accgaagtct ctcttcagga agctcgccag
     4141 ttcgacagcg aggcagaggt cggtgatgag attgggtcgc tcatcgaaat gagtgactta
     4201 ggacggatcg ccgcgcaaac cgccaagcaa gtcatcttcc agaaggtacg ggaggccgaa
     4261 tgggaggcgg ttcaaaagga atattccaca cggcaagggg atctggtcaa cggcatcata
     4321 ctcgggatgg agcgccggaa ttacctggta gatttgggca agac
//
LOCUS       DATIPF010000233         3515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_664474, whole genome shotgun sequence.
ACCESSION   DATIPF010000233 DATIPF010000000
VERSION     DATIPF010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664474"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VLL06_05935"
     CDS             <1..696
                     /locus_tag="VLL06_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSS30540.1"
                     /translation="AGCDNLTQSSWFPSILNQTEKLTERVQRAVTPSNALAKEYGDAD
                     ISNVFPANGSTDPGTEDYAEHVAKQFFEWTVEVGGLVQTPMSWSFAALKELPSRTQVT
                     RHDCVEGWSAIGKWTGVPLGDLMHRVEPLPSAKFAVFHCADVDDEGVAYYESMALADC
                     YHPQTILAYELNGNPLDVPHGAPLRLRFERQLGYKHAKYVMRIELVESLAGIGGGRGG
                     YWEDQGYEWYAGI"
     gene            849..1541
                     /locus_tag="VLL06_05940"
     CDS             849..1541
                     /locus_tag="VLL06_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HSS30541.1"
                     /translation="MAELRALNSGKKSATVKDPAALTTQLAAGLEKIGLAMKSRTWRR
                     EGRAGLGPLQRQVLTLLQSKPEQRATVSTVANELSVRLPTASEVIATLERKQLVRRRR
                     DMSDRRIVVAQLTAKGNRSCTPSSRMPDRLATATAALSPPEQVVLLTSLVKMIRSLQE
                     QGEISATRMCVSCRHFHPNQHANADRPHHCDYLKIPFGDGSLRLNCQVYEPASTAQVN
                     ETWANFTGSRTA"
     gene            1606..1830
                     /locus_tag="VLL06_05945"
     CDS             1606..1830
                     /locus_tag="VLL06_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30542.1"
                     /translation="MGDGKTTILFKKASTRPSEDEWLGPESHMPNLNRELRRREPRRM
                     GFSSGWMVGVMFAAGVAAGCLAMRLFDRRS"
     gene            complement(2104..2661)
                     /locus_tag="VLL06_05950"
     CDS             complement(2104..2661)
                     /locus_tag="VLL06_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30543.1"
                     /translation="MNDGRLLHQFSRIVLGGLLLISWGCVHRIQVTPVPPAVSSITIG
                     QSVEVIVPFLALKGADHRQGITLLEWPAQDLRSATITYIQSRQTFTSVSDTPADLTLT
                     VKAWLTMRSSGNYRYRLRLESALGPSGKLPVKSYLVEKEAIGSSVRWSTASDQAPIAE
                     TVQAALDDLLTQIEADHLLYRNGAR"
     gene            complement(2658..3146)
                     /locus_tag="VLL06_05955"
     CDS             complement(2658..3146)
                     /locus_tag="VLL06_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30544.1"
                     /translation="MTRPPFAPYRSILVIACCLILASCAHDTYQDRANLIKSHAESFY
                     DNLKTNRVESAIRNNEQIEAMASEMGDTVRKRAGQQGTTSVEREFALMNTASEAAATN
                     WLALGQYFTIKRQYPQARSTYRRVIDTYTNAADRPYREQALRALKDLDMLSPPTSTTT
                     NP"
     gene            3323..3400
                     /locus_tag="VLL06_05960"
     tRNA            3323..3400
                     /locus_tag="VLL06_05960"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3359..3361,aa:Glu,seq:ttc)
BASE COUNT          718 a          935 c         1073 g          789 t
ORIGIN      
        1 gccggctgcg acaacctgac gcagagcagc tggtttcctt cgatcttgaa tcagacggag
       61 aaactcaccg aacgagtgca acgggccgtc acaccctcga atgcccttgc gaaagaatat
      121 ggggatgccg acatctcgaa cgtgtttccg gcgaacggga gtaccgatcc cggcacagaa
      181 gactatgccg aacacgtggc gaaacaattc ttcgaatgga cggtggaggt cggcggcttg
      241 gttcagaccc cgatgagttg gtcgttcgcg gcgttgaaag aattgccctc gcggacacag
      301 gtcacccgcc acgattgcgt cgaaggctgg agtgcgatcg gcaagtggac cggcgtcccg
      361 ctcggcgatc tgatgcatag ggttgagcca ttgccgagtg ccaagttcgc cgtctttcat
      421 tgcgccgatg tggacgatga aggcgtggcc tactatgaaa gcatggctct ggcggactgt
      481 tatcacccgc agacgatctt ggcctatgag ctcaacggca atcctctcga tgtaccgcac
      541 ggggcgccgc tccggcttcg ctttgagcgg caactcggct ataagcatgc caagtatgtg
      601 atgaggatcg aattggtgga gtcgcttgcc ggcatcggcg gcgggagggg tgggtattgg
      661 gaggatcaag ggtacgagtg gtacgcgggg atctaaccag gatgttgaaa ctagccgcca
      721 actgcgttct cgttcttccc tccggcccgc tgatagagct ggtttcaggt agtgccatcc
      781 ggaatcttga cgtggaggca gtaagcgcac tacacttacg aaatactggt atttaaggag
      841 gagcttttat ggctgaactg cgggcgctca atagcgggaa gaagtcggcg acagtgaagg
      901 atccggctgc gttgaccact cagttggcgg caggcctgga aaagattggc cttgcgatga
      961 aaagccggac gtggcggcgc gaaggtcgag ccggtcttgg cccattgcaa cgtcaggtcc
     1021 tcacgctgct tcagtccaag cctgagcaac gcgcgacggt ctccacggtg gccaacgagt
     1081 tgtccgtcag gcttcccact gcgtcggaag taatcgccac gcttgaacga aagcagttgg
     1141 ttcggcggcg gcgggatatg agtgatagac ggattgtcgt tgcgcaattg acggccaaag
     1201 ggaatcggtc ctgcacgccc tcttcccgta tgcccgatcg gttagccact gcgacagcgg
     1261 cgctttcgcc tccggagcag gttgtcctcc tcacgtcctt ggtgaagatg attcgcagcc
     1321 tccaagaaca gggtgagatt tctgccacga ggatgtgtgt gtcttgtcgg cattttcatc
     1381 ccaatcaaca tgcgaatgcc gatcggccgc accactgtga ctatctgaag attccgttcg
     1441 gcgatgggtc gttacgtcta aattgccagg tctacgaacc agcttcgacc gcgcaggtca
     1501 atgagacctg ggcaaacttt accggttctc gtacggccta ggatccgtgg ctcccgctgt
     1561 gttgggagga tcggtatcca tgggcttgga gggaaggagg ggatcatggg tgacggaaag
     1621 accaccattc tcttcaagaa agcttcgacc cgtccatcgg aagatgaatg gctcgggccg
     1681 gaatcccaca tgccgaatct gaatcgggaa cttcgacgcc gtgaaccgag gagaatgggt
     1741 ttctcatccg gttggatggt cggggtcatg ttcgcggcag gtgtggcagc gggatgtctg
     1801 gctatgcgcc tattcgaccg acggtcttga acctctgaac aggatgctga aacgctccgc
     1861 cagcttcgtt ctcggctcat cgaaatcctc aacgtacccc cccgggggcc tccggtttca
     1921 actcgcctgc ggccttgctt gggagaagac gcgtcttggc gcgccagggt ttggcgggtg
     1981 agatgtccgg accttttgag catcctgcgg ggatgcagtt ctcttattcc agacgtgtag
     2041 gcgatcgaag atccgctccg ccacaatgat ttttttgtgc cggtccagtc tctgccgttt
     2101 tgttcaccga gccccatttc tatataacag gtgatcggct tcgatctgtg tgagcagatc
     2161 gtccagcgcg gcttggaccg tttctgcaat gggggcctga tccgaagccg tgctccaccg
     2221 aacactcgac ccgatcgctt ctttttccac cagataggat ttgaccggga gctttcccga
     2281 aggaccaagg gctgattcca ggcgcaaacg atagcggtag ttcccactgg atcgcatggt
     2341 cagccaggct ttgacggtca gtgtgaggtc ggctggtgtg tcgctcaccg aggtaaatgt
     2401 ctggcgcgat tggatgtagg tgattgtggc ggaacgaaga tcctgtgctg gccactcaag
     2461 caaggtaatg ccctgcctgt ggtcggctcc tttcagcgcg agaaagggca cgatgacctc
     2521 taccgattga ccgatggtga tagacgaaac ggctggaggc acgggggtga cctgaatccg
     2581 gtggacacac ccccagctga tcaacaggag cccgccgaga acgatgcgag agaattgatg
     2641 cagaagacgg ccgtcgttca cggatttgtc gtggtcgagg tgggaggact gagcatatcc
     2701 aaatccttca aggcgcgcag ggcctgctct cggtatggtc ggtccgcggc attcgtatac
     2761 gtgtcgatca cacgtcgata ggtggagcga gcttgggggt actggcgctt gatggtgaag
     2821 tattggccca gcgcgagcca gtttgtggcg gcggcttcgc tggcggtgtt catcaaggca
     2881 aactcccgct cgaccgatgt cgtgccttgt tgaccggccc gtttcctgac ggtgtctccc
     2941 atctcgcttg ccatcgcttc aatctgttca ttgttccgga tcgcggattc cacccgattg
     3001 gttttcaggt tgtcatagaa tgactcggca tgactcttaa tcaggttcgc tcgatcttgg
     3061 taggtatcgt gagcacagct cgccaggatc aggcagcagg caatgaccaa gatggatcgg
     3121 tacggtgcga atggaggtct tgtcatgaat cgtgctcctc gggtccttcc aacgtgaggc
     3181 tccaacatac ggtggagtca aatcatccta ccgaattcga agaagaaacg cgactgggtg
     3241 cagggaagcc tcccgtgtcc tctagtgaca cttgagtctc aagtatggta agagtccagt
     3301 tcatttttgt aggccctgct tcgtccccat cgtctagcct ggcctaggac attgcccttt
     3361 caaggcagta acacgggttc aaatcccgtt ggggacacca aggcctttgc acgacctgtc
     3421 accagcctac caatcgcagc cgtaccttta aattcatact tggagttccc ggcttcagga
     3481 agtggataga acggaaggcg agtaccattg aagag
//
LOCUS       DATIPF010000234         3776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1638383, whole genome shotgun sequence.
ACCESSION   DATIPF010000234 DATIPF010000000
VERSION     DATIPF010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638383"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="VLL06_05965"
     CDS             complement(<1..1299)
                     /locus_tag="VLL06_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HSS30545.1"
                     /translation="MNLVDLIEGADPLLVSGRVAQAVGIVIEGYGPMTSVGELCDVTR
                     EDGEGIVPAEVVGFRGDRVLLMPLGEMRGIGPGSRLLMKGQCASVSVGPQLLGRVLNG
                     LGEPLDGRGPFMTEHQYPLHAAPINPLMRTRIAEPLDLGIRAINACLTCGRGQKVGIF
                     ASSGVGKSVLLGMISRYTKADVNVIALIGERGREVNEFLERDLTPEALGRSVVIVATS
                     DQPPLVRLRGALIATTIAEYFRDCGKQVLLMMDSLTRLAHSQREVGLAIGEPPTTKGY
                     TPSVFTMLPKLLERVGTGPNSGAITGLYTVLMESDELSDPLAEAVRSILDGHIVLSRQ
                     LAARNHFPAIDLLNSTSRVMKDIVRRDHYTAVRSMIELQARYQQSEDLIMLGAYKAGT
                     NRDLDKAVEAQDGINAFLRQDIEESVSIGTSIQDVLTLMKT"
     gene            complement(1277..2335)
                     /locus_tag="VLL06_05970"
     CDS             complement(1277..2335)
                     /locus_tag="VLL06_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS30546.1"
                     /translation="MEHASNGHATLAQPRRNLSGRREGAILVVDDEQAIRELLVRLLQ
                     SAGHRVRAVGSAREAYAALKESPVSLLITDVKLPDEDGIALLQQACDFDHRMMGLVMT
                     GYGTVDLAVQAMKAGAAEFLMKPFETEALQLTVNRLLDLYYLRSENTVLKQAVIRASG
                     VRCRSLALTDFDTGDRFVGDQTISDFDQGIAEGERRADARVAAARHQEQMLLANAVRR
                     FDETWTSLHQTVEDDVTALAFSLATRILRDTMIERQDAVIALLKSALATVREAGVVTV
                     RTHPSDAPIFEAARSELCRDRPTAFAMHVEGDSSLSPGSCVVQSTNRLVDASLDTQLL
                     RLGEILQKRGRRESRRSH"
     gene            complement(2335..3333)
                     /gene="fliG"
                     /locus_tag="VLL06_05975"
     CDS             complement(2335..3333)
                     /gene="fliG"
                     /locus_tag="VLL06_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HSS30547.1"
                     /translation="MATHLSGEQKAAILLKAIGEDAAAMVMKSLTPKEIRRLGQSMNG
                     TANITREEESIVIDEFDKASASGDVTFQGKEYIKAILSKALGPEKANRMIESMTNKVY
                     PGLDAMKWVDAKAVSQMIKTEHPQTISVILAHMDPDQAGTVMVDLPEALRADVALRLA
                     TMEDLQPSVLEELSDALQSSLVASTGTRATSVGGAEAIANILTTLDKATEGGIMSKIA
                     ERDQILADTIRGLMFVFDDLIKLDDRGMQELMKEVSKEDLPRALRGAAPETKEKFLKN
                     MSSRAAEMLKDDMESMGPVKVADVERAQQNILKVCRKLEEEGRIVIAGAGGEEAML"
     gene            complement(3347..>3776)
                     /locus_tag="VLL06_05980"
     CDS             complement(3347..>3776)
                     /locus_tag="VLL06_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HSS30548.1"
                     /translation="VARPEEDMKRIEEIVKKAMGYSAERQDQVQVTNVQFDIATEEPP
                     AQGVEAAAETTSSFIPYIRYGVGGGLFLLILFMVVRPLMGMLGTIGSSGEGSTPTLPA
                     SVSQVEASLTGKPRSQIVDMARGNPDATAVVVKQWLKTNP"
BASE COUNT          742 a         1188 c         1026 g          820 t
ORIGIN      
        1 cgtcttcatg agtgtgagta cgtcctgaat cgaggtgccg atcgagacgg actcctcgat
       61 gtcctggcga agaaaggcat tgatcccatc ttgagcctct acggctttgt cgagatcgcg
      121 attggtgccc gccttatagg cgccgagcat gatgagatcc tcagattgtt ggtaccgggc
      181 ctgcagctcg atcatggacc ggaccgccgt atagtggtcg cgccgtacaa tgtccttcat
      241 cacccggctg gtgctgttta agagatcgat cgccggaaaa tggttacgcg cggccaactg
      301 tctcgacagc acgatgtggc cgtccaggat cgaccggacc gcctctgcaa ggggatcgga
      361 gagttcgtca ctctccatga gcaccgtata gagcccggta atcgcgccgg aattggggcc
      421 ggtcccgact cgttccagga gcttgggcaa catcgtaaag acggaggggg tgtacccctt
      481 cgtggtcggt ggttccccga tggcgagtcc gacttcacgt tgactatggg ccaggcgggt
      541 gagtgaatcc atcatcagga gcacctgctt gccgcagtcg cggaaatatt ctgcaatcgt
      601 cgtggcaatc aacgcacccc gtaatcgcac gagcggtggc tggtctgatg tcgctacgat
      661 caccaccgaa cgaccgagcg cctccggggt caaatcgcgt tcgaggaatt cgttgacctc
      721 ccgtccccgc tcgccgatga gggcgatgac gttcacgtcg gccttcgtgt agcggctgat
      781 catcccgagc aacacactct ttccgacacc ggagctggcg aagatgccga ccttttgacc
      841 acgtccacag gtcagacagg cattgatcgc tcggataccc aggtcaagcg gttctgcaat
      901 acgcgttctc atcagaggat tgatcggcgc ggcatgcaac ggatattggt gttcggtcat
      961 aaacggaccg cgcccatcca atggctctcc cagcccgttg agcacgcggc cgagcagttg
     1021 cgggccgacc gaaaccgagg cgcattgtcc tttcatgagc aatcggctgc cgggaccgat
     1081 cccacgcatc tccccaagcg gcatcagtaa cacgcggtcg ccgcgaaacc cgacgacttc
     1141 cgccggcacg attccctctc catcctcgcg ggtgacatcg cacaactccc cgaccgatgt
     1201 catggggcca taaccttcga tcacaatccc cacggcctgg gccacacggc ctgagaccag
     1261 aagaggatct gccccctcaa tgagatcgac gagattcacg gcggcctcgc ttttgtaaga
     1321 tctctccgag tcgtagcaac tgcgtgtcca gcgaggcatc aaccagtcga ttcgtggatt
     1381 gcaccacaca gctgcccggg gagagagacg aatcgccttc gacatgcatc gcaaatgccg
     1441 tggggcgatc gcggcacaac tccgatcgag ccgcttcaaa gataggggcg tcggatggat
     1501 gcgttcgcac ggtcaccacc ccggcttcac gcaccgtcgc caaggccgac ttcagcaatg
     1561 cgatcacggc atcttgacgt tcgatcatcg tgtcgcgcag gatgcgcgtc gccaggctga
     1621 aggccagcgc cgtcacgtca tcttcaacgg tctgatgaag cgatgtccat gtctcgtcaa
     1681 agcgccgaac ggcgttggcc aagagcatct gctcctggtg cctggctgcc gcgactcgtg
     1741 cgtcggctcg tcgttcaccc tccgcaatgc cttgatcgaa atccgaaatg gtctggtcgc
     1801 cgacgaatcg atcaccggtg tcgaaatcgg tcagcgcaag actccggcag cgaaccccgg
     1861 aagcacgaat caccgcctgc ttgagaaccg tattctccga acgcaggtaa tacagatcga
     1921 ggagacgatt cacggtcagc tgcaacgcct cggtttcgaa cggcttcatc agaaactcag
     1981 ccgctcctgc tttcatcgct tgcaccgcaa gatccacggt gccatatccg gtcatcacca
     2041 gtcccatcat acggtgatcg aagtcgcagg cctgctggag cagcgcgatg ccgtcttcgt
     2101 ccggcaattt gacgtcggtg atcaggaggg agactggaga ctcttttaat gccgcatagg
     2161 cttcccgtgc ggatcccacc gcacgcacac gatgccccgc cgattgcagc aaccgcacca
     2221 acagttcacg gatggcctgc tcatcgtcga cgacgaggat cgcaccttct cgtcgaccgc
     2281 tgagattccg gcgtggctga gcgagtgtcg catgtccgtt cgacgcatgc tccattacag
     2341 catcgcctcc tctccacccg cgccggcgat gacgatacgc ccttcctctt caagtttccg
     2401 gcagactttg aggatattct gctgcgccct ctcaacatcc gccaccttta ccggtcccat
     2461 tgactccata tcgtctttca gcatctctgc ggcccggctc gacatatttt tgaggaactt
     2521 ctccttggtt tccggagccg ctccacgcaa tgctcgggga agatcctctt tactgacctc
     2581 tttcatgagc tcttgcatgc cacggtcatc gagtttgatc aggtcgtcga agacgaacat
     2641 caagcctcga attgtatcgg ccaatatctg atcacgttcg gcgatcttcg acataatgcc
     2701 gccctctgta gctttatcga gggttgtaag aatattggca atcgcctcag cccctccgac
     2761 gctcgtcgca cgggtgccgg tactcgcgac cagtgaactc tgcaacgcat cgctgagttc
     2821 ttcgagcaca ctcggctgca agtcctccat cgtcgcgagg cgcagggcga catcggctcg
     2881 aagggcttcc ggcaggtcga ccatgacggt gccggcctga tccggatcca tgtgcgccag
     2941 gatcacggag atcgtctggg gatgctcggt cttgatcatt tgtgagacgg ccttggcatc
     3001 gacccacttc atcgcgtcga gtcccgggta gactttattg gtcatggact cgatcatacg
     3061 gttggctttt tcaggaccga gtgccttgga cagaatcgct ttgatatatt cctttccttg
     3121 aaacgtcaca tccccgcttg cgctcgcctt atcgaattca tcgatgacga tcgattcttc
     3181 ctcccgcgtg atgtttgccg tcccgttcat agactgtccc aaccgacgaa tctcctttgg
     3241 agtaaggctc ttcatgacca tcgcagcggc gtcttcccca atggctttaa ggagaatggc
     3301 cgctttttgc tcgccgctga ggtgcgtcgc catcgctata cgtgcctcat gggttggtct
     3361 tcaaccattg cttgacgact accgccgtgg catccggatt gccccgggcc atgtcgacga
     3421 tctgtgagcg cggctttccc gtcaacgacg cttcgacctg actcactgaa gcgggaagcg
     3481 tcggtgtgga cccctcaccc gatgacccga tcgtacccaa catccccatc agggggcgca
     3541 ccaccatgaa gagaatcaag aggaagagac ctcccccaac cccatagcga atgtagggga
     3601 tgaaggaact cgtcgtctct gccgcggcct ccacaccctg cgcgggaggt tcttcggttg
     3661 cgatgtcaaa ttgcacgttt gtcacctgga cttgatcctg ccgctctgcc gaatagccca
     3721 tggctttctt gacgatctct tcgatgcgct tcatgtcttc ctcaggccgt gccaca
//
LOCUS       DATIPF010000235         1530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_840684, whole genome shotgun sequence.
ACCESSION   DATIPF010000235 DATIPF010000000
VERSION     DATIPF010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840684"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VLL06_05985"
     CDS             <1..346
                     /locus_tag="VLL06_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HSS30549.1"
                     /translation="ERAIECSANATARAVAIRATRKWGKIAFVGEGGTVDFNPSPDII
                     HDQKTIYGSWVTSTWLMEELVERLVRWNLHPADIITHRFGLEHAAGAYALMASGKCGK
                     VAVCADEELEGK"
     gene            343..885
                     /locus_tag="VLL06_05990"
     CDS             343..885
                     /locus_tag="VLL06_05990"
                     /inference="COORDINATES: protein motif:HMM:NF016885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HSS30550.1"
                     /translation="MKTNWEETLKDRLEAFGHRNWLVIADSAYPAQSKEGIETIVADE
                     EQTAILDKVHMILSASQHVRATIYTDKELTFVREEDAPGVTSYRKRLASLFEGYEVQP
                     LPHETIIWKLDRVAAKFRVLLIKSNMRIPYTSVFFELGCGYWNAQAEKRLRAAMRSRD
                     RRRKTPRRTNGSSLLSEAHS"
     gene            1218..>1530
                     /locus_tag="VLL06_05995"
     CDS             1218..>1530
                     /locus_tag="VLL06_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30551.1"
                     /translation="MQRDGALTEFIVMPPEKLYPAKLTPKELCLVEPLTVGFHAVSRG
                     RVTGEDTVAIFGCGGVGLGAIAASTSRGARTICFDLDDEKLRVARLAGATHIVHSLRE
                     RV"
BASE COUNT          354 a          384 c          461 g          331 t
ORIGIN      
        1 cgagagggcg attgaatgtt ctgcaaacgc aacagcgcgt gccgtggcaa tacgggcgac
       61 gcgcaagtgg gggaagatcg cgttcgtcgg cgaaggtggt acggttgact tcaatccctc
      121 acccgacatc attcacgatc agaagacgat ctacggatcg tgggtaactt cgacatggct
      181 gatggaagag ctagtcgagc gcctcgtccg ctggaatctc catccggccg acatcattac
      241 gcaccgtttt ggccttgagc acgctgcagg cgcgtatgcg ctgatggcgt cggggaagtg
      301 cggcaaggtg gccgtgtgcg ccgatgagga gttggaaggt aaatgaagac aaactgggaa
      361 gagactttga aagatcggct ggaagccttc ggccatcgca actggctcgt gatcgcggat
      421 tcggcatatc ccgctcaatc caaagaggga atcgaaacca tcgtcgccga tgaagaacag
      481 accgcaattc tggacaaggt acacatgatt cttagcgcga gtcagcacgt tagggccacc
      541 atctacacgg ataaggaatt gacgtttgtc cgagaagagg acgcgccggg tgttacttcg
      601 taccggaaaa ggctcgccag tctttttgag ggctatgaag tgcaacccct tccacacgag
      661 accatcattt ggaagcttga tcgggtcgcc gcaaagttcc gcgtactgtt aatcaaaagc
      721 aacatgcgta ttccatatac gtcagtattt ttcgaactcg ggtgcggata ttggaatgct
      781 caggccgaga agcggcttcg ggctgccatg aggtccagag atcgtcgccg aaaaactccc
      841 aggcgaacga acggtagctc acttttgtct gaggcccact cttaaccatg aaggcgttcg
      901 tgctcaagaa acccgggcag gcatctatcg agactatccc tgatccggtg gccatgaatg
      961 ggaacatact gctcaaggtc cgaatggtgg gattctgtgg cagtgatcta aattcgtttc
     1021 gtgggagtaa tccacttgtc tcctatccac ggattcttgg gcatgaagtg tcggccacag
     1081 tcgcgcaagg atccgacccc gaccggggac tatcggttgg aacagacgtt gccatatctc
     1141 cttatacaag ctgcggaaca tgtgcgtcat gccagcgcgg acgctcgaat gcgtgtcagt
     1201 tcaacgagac tctgggtgtg caacgagatg gggcactgac cgaattcatc gtgatgccgc
     1261 cagagaagct ttatccggca aagctgacgc ccaaggaatt gtgtctggtg gagccgctga
     1321 cggtcggatt tcatgccgta tcgcgtggac gagtcacggg ggaggatacg gtcgcgattt
     1381 tcggctgcgg gggagttgga cttggtgcga tagcagcctc tacttctcgg ggtgctcgaa
     1441 cgatctgctt tgacttggat gatgaaaagc taagagttgc tcgacttgcg ggcgcgactc
     1501 acatcgttca cagcctcaga gagcgggtcc
//
LOCUS       DATIPF010000236         4329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_487307, whole genome shotgun sequence.
ACCESSION   DATIPF010000236 DATIPF010000000
VERSION     DATIPF010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487307"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VLL06_06000"
     CDS             complement(<1..552)
                     /locus_tag="VLL06_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSS30552.1"
                     /translation="MTSRVTRAAKAERMNESRRLLFARTASTVVLVCLLLAACGRGEE
                     PAVSNAAATTPPKVGLTVESHPRVETALVQLGSGAHDLTLVGRVAYAEDHYSRISSPL
                     QGRVLEVRAHLGEHVEAGSVLLVLDSQEIAQAYSEYAKEDSDLQFATRAHELAKDLYE
                     NRALALKDLKQAENELIKARAEFR"
     gene            complement(549..1853)
                     /locus_tag="VLL06_06005"
     CDS             complement(549..1853)
                     /locus_tag="VLL06_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSS30553.1"
                     /translation="MMPLICYMIVLSVLSSSAYLSHASASSTSPTPDHVTTAPKALRL
                     SLTDALGLFLSQNLDVLMAKYGIEYRKGQEVTARLFPNPVLSVGTQGSNTQGRTLSNS
                     GQLFTQASQLFELAGKRGYRIESAGFGTQSAEAAFEDAVRQLGFTVKDNYYRVQLAQR
                     RLVLAEENRERFSRILDINTIRFKKGYIAEVDLIRIRLQMVDFHSQVLQALQEAESAR
                     GDLRQLLRLSPATALELTTELGFRRVDPDIEKLQTAALAMRPDIRAKRHTLSQRESEL
                     KLAMAYRIPDVTIGAGYAIQGPRGPDNPGQFALNAGIPLPLFNRNQGGIHQAEVSLQS
                     AQADLDKTVNLVENEVEVAYRNLLQNRRLVEAYFGEVLEDARSTFTIVERAYERGGAT
                     ILDLLDAARTSRTIQQNYIEALFNYQHHLFQLESAVGQEILL"
     gene            2304..>4329
                     /locus_tag="VLL06_06010"
     CDS             2304..>4329
                     /locus_tag="VLL06_06010"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSS30554.1"
                     /translation="MQMPREKYIWIGLGIALLLFLSMTVVVYLSTTGFMQTASHVARS
                     HEIRLTFEHIRTLIDDIETGHRAYVISGEAILLEPYHGAVNLIRNKLKQARELTADTP
                     RHQEHLNRLDKLVASRLDVAEQSITIARTQGIATARTLLQSEQGSREIEEIHRLLTES
                     IDDEVGEISEHSKRSTALAESTIMFVGGGSLLVLAFVLGAGGVYKLDLSRRQRTEEAL
                     RASETKQALIMRTLSVASYSALASGNFGALWVSDNIDVITGFPGTTFLQDADLWASRL
                     HPADREQVLLAFQRIPNTGTVSTEYRWRVADGTYRWFLDNAVLQKNPDGTAKEILGIL
                     IDITLQKEIEDRLRQSNDRLTALIHASPIGIVILDADGLCQLWNPGAEHIFGWREDEV
                     LGRPLPTVAPEQSDEHQFLRERVMKDEAFTDLEVVRYKKDGTPVFISLSTAPLRDSSG
                     TICGVLGLMADMTQRKQVGLELSHSRDQLRALAKRLESVREEESSRIAREIHDELGQA
                     MTSLKLDLSWVARRLSIPETADTRRQLLERIQGTMQQLEVTIQTVRAIATTLRPSVLD
                     ELGLAAALDWQTRDFEKRTGIRCEWSMPLVPIPIGPDQATAIFRIYQEILTNVVRHAQ
                     ASNIRIHLGICAGWLVFEVCDNGRGIPDSVLVNQNSLGLLGMRERAAQWGG"
BASE COUNT          985 a         1222 c         1209 g          913 t
ORIGIN      
        1 gcggaattca gcgcgcgcct tgatcaattc attttccgcc tgcttgaggt ccttcaacgc
       61 cagtgctcta ttctcataca ggtccttggc caactcgtga gcacgtgtcg cgaactgaag
      121 atccgagtct tccttggcat attccgaata ggcctgggca atttcctggc tatccaacac
      181 cagcagcaca ctcccggctt ccacatgctc gcccaaatgc gcccgcacct caaggactcg
      241 tccttggagt ggggaggaaa ttctggagta gtgatcctcc gcgtaggcga ccctcccgac
      301 caatgtgaga tcatgggctc ccgacccgag ttggacgagc gccgtttcca cccgcggatg
      361 cgattccaca gtcaatccga ccttcggagg tgtcgtcgcg gcggcattgg agaccgccgg
      421 ctcttcccct ctcccacagg cagcgagcaa gaggcagacg aggacgaccg tcgaagccgt
      481 ccgcgcgaat aggagtctgc gagattcgtt catacgctcg gccttcgccg cacgcgtgac
      541 ccggctcgtc acaacagaat ctcctgccct acggcacttt ctaattggaa gaggtgatgc
      601 tgataattaa ataaggcttc gatatagttt tgctgaatcg ttcgcgaggt gcgagcggca
      661 tcgagcaaat cgagaatcgt cgcaccgccc cgttcatagg cccgctcgac aatcgtaaac
      721 gttgaacggg cgtcttccaa cacctcgccg aaataggctt cgaccaatcg ccgattctgc
      781 agaaggttcc ggtaggcgac ctccacctca ttttcaacaa ggttgacagt cttgtccaag
      841 tcggcttgcg ccgactggag agaaacctcc gcttgatgaa tccctccttg attccggtta
      901 aacaggggca acggaatacc ggcgttgagc gcaaattgac caggattgtc aggcccccgt
      961 ggtccctgaa tcgcgtagcc ggctccgatc gtcacatcag gaatccgata ggccatggcc
     1021 agtttcagct ctgattcccg ctgagacagg gtatggcgtt tggcgcggat gtccggacgc
     1081 atcgccagtg cggccgtctg caacttttcg atatcgggat cgacacggcg aaaacctaac
     1141 tccgtggtca attccagcgc cgtcgccgga gataaccgca gcaactgacg aagatcgcct
     1201 ctcgccgatt ctgcctcctg cagtgcctgg agcacctgag aatgaaaatc caccatctgc
     1261 aagcgaatcc gaatcaggtc aacttcggca atgtacccct ttttgaaccg aatggtattg
     1321 atgtcgagga tacgggaaaa acgttcgcga ttctcttctg ccagtaccag tctccgttga
     1381 gccaactgca cccggtaata gttatccttg acggtaaacc cgagctgccg caccgcatct
     1441 tcgaaagccg cttccgccga ttgtgtgcca aaccccgcac tttcgatgcg atagccccgt
     1501 ttcccagcca attcgaacaa ctgagaggct tgtgtaaaca gctgcccgct attcgacagg
     1561 gtcctccctt gcgtattgga gccctgtgtt ccgactgaca acaccggatt cgggaagaga
     1621 cgtgcggtaa cttcctgccc ttttctatac tcaatcccgt acttggccat gagcacatca
     1681 aggttttgac tgaggaacag tcccagcgcg tcggtcaagc tgagacgaag tgcctttggc
     1741 gccgtggtca catggtcagg agtcggagat gtcgagcttg cgctggcgtg ggaaagatat
     1801 gcagaactgc tcaacacgct gagaacgatc atatagcaga tgagaggcat catcgaccca
     1861 caaacacaga ttgggaccga gataaaccgt cgcatggacg agggcctgag cacaattgac
     1921 cgcaccatcc tctcaactaa gtctgtagta taccgtgcat gccaaacgaa gcaaagaacc
     1981 tcttcaaaag gacaagccca tgagcgcctc tccgtataga gacgttcttg tggagcggga
     2041 aatccaccta gaaacaatct tgtgcctaag caaagcggtg gtcgtcgtct atgcgcagtt
     2101 gtttgcattg taggacggcg cttacaatca gacttctcag atgctgatag cctgggcctg
     2161 ttcaaagcat tagggtactg tgctgaatgc gcgtgacacg tcgtacacag acacatgtgc
     2221 atgaggagcg agcgttctgc aagcagaggc gatattgact ggctacgcgg gatgtgagct
     2281 aacctacgca aagaagtgac atcatgcaga tgccccgaga gaaatatatt tggatcggct
     2341 tagggattgc gcttctgctg tttctcagca tgacggtggt cgtctacctc agcacgaccg
     2401 gattcatgca aaccgcctct cacgttgccc gaagccatga gatccgtttg acgttcgaac
     2461 atatcaggac cttgattgat gatatcgaga ctggccatcg tgcctacgtg atttccgggg
     2521 aagccatcct gttggaaccc taccacggag cggtgaacct gattcgaaac aaactcaagc
     2581 aggccagaga gctgaccgca gacacgcctc gccatcagga acatcttaac cgactcgaca
     2641 agttggtcgc gagccggctc gacgttgccg agcagtcgat cactatagcc cgcacccagg
     2701 gaatcgcgac ggcacgtacg ctcctacagt cagaacaagg cagccgagaa atagaggaga
     2761 tccatcggct cctcacggaa tctatcgatg atgaggttgg cgagatatcg gagcactcca
     2821 aacgcagcac ggccttggcc gaatcaacca tcatgtttgt gggaggagga agtctcttag
     2881 tcctggcctt cgtgctggga gcgggagggg tctacaagtt ggatctctcg aggcgtcaac
     2941 ggaccgagga ggcgctgagg gccagtgaaa cgaaacaggc gttgatcatg cggactttgt
     3001 cagtggcatc ctattccgct ctggcgtcag ggaatttcgg ggcactttgg gtgagcgaca
     3061 atatcgacgt gatcaccgga ttccccggca ccaccttcct gcaggatgcg gacctctggg
     3121 cttcgcgtct ccatccagcc gatcgtgagc aagtcctgct cgcatttcaa cgaatcccca
     3181 atacaggcac cgtgagtacc gaatatcgat ggcgagtcgc cgatgggacc tatcgctggt
     3241 tcctcgacaa cgccgtgctt cagaagaatc cggacggcac cgcgaaggag atccttggca
     3301 tcttgatcga cattaccttg cagaaggaga ttgaggatag gctccggcaa tccaacgacc
     3361 ggctcaccgc actcatccat gcgtcgccga tcgggatcgt gatccttgat gccgatggac
     3421 tctgccaact ctggaatcct ggggcggaac atatcttcgg ctggcgcgaa gacgaagtgc
     3481 tggggcgccc ccttccgact gtggcgccag agcaatcgga cgagcaccag ttcttgcggg
     3541 aacgagtgat gaaagacgaa gcgttcacgg atctggaagt cgtacgatac aagaaagatg
     3601 ggacacctgt ctttatcagc ctgtcgacag cgcctcttcg ggattcaagc ggcaccattt
     3661 gcggagtgct cgggttgatg gccgatatga ctcaacgcaa gcaagtcggg ctcgagctca
     3721 gccactcccg agaccaacta cgggccttgg cgaaacgatt agaatccgtg cgggaagagg
     3781 aaagttcgcg gatcgcccgt gagatccacg atgagttggg gcaggccatg accagtttga
     3841 agcttgatct atcctgggtg gcaagacggc tctccattcc ggagacggct gatactcgga
     3901 ggcaactgct ggagcggatc caggggacga tgcagcagct tgaagtcacg atccagacgg
     3961 tgcgcgcgat cgcgacgacc ttgcggccca gtgtgttgga tgaacttggc ctggccgccg
     4021 ccctcgattg gcagacgcgt gactttgaga agcgaaccgg catacgatgc gaatggtcga
     4081 tgccgttggt ccccattccg atcggaccgg atcaggccac ggcgatcttt cgtatctatc
     4141 aggagatcct caccaatgtg gtgcgtcatg cccaagcctc caacatccgt atccacttgg
     4201 gcatttgcgc gggttggctc gtgttcgaag tgtgcgataa tggtcgaggt attccggact
     4261 ctgtcctggt taaccaaaat tccttggggc tccttggaat gcgagaacgg gctgcgcaat
     4321 ggggtggcg
//
LOCUS       DATIPF010000237         2944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_133609, whole genome shotgun sequence.
ACCESSION   DATIPF010000237 DATIPF010000000
VERSION     DATIPF010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133609"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..888
                     /locus_tag="VLL06_06015"
     CDS             151..888
                     /locus_tag="VLL06_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HSS30555.1"
                     /translation="MLTGLSNAVQNQHILTSALFDDVAERQGQGSVAALFDVDNTLLP
                     GEASEIGFFRFLWRRGLVGWGELTRSAAWLAGHVPPFSLHALRERKVYLTGKCPADIE
                     SHAREFCQAEMIDKLSLQGRTRLDMHRQAGHQLILVTGAPEFLVVPLAKYLGISTVFA
                     AKPEERDSVYTGALIPPLPYGRGKRELILAHAQDMNLDLPGSYAYGDSPGDRDILELV
                     GHPLVVNPIRGMARTAQERGWPVTLWK"
     gene            995..1630
                     /gene="queE"
                     /locus_tag="VLL06_06020"
     CDS             995..1630
                     /gene="queE"
                     /locus_tag="VLL06_06020"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HSS30556.1"
                     /translation="MRITEIFHSIQGESSYAGRPCVFVRLTGCPLRCTWCDTDYAFYG
                     GRECSIEEVLAQVQNYGCRLVEFTGGEPLVQPESFPFLTRLCDAGYTVLLETSGAVDI
                     APVDPRTHVVLDVKCPGSGMTDRMHWPNLARLTAKDEAKFVLADRVDYDWAREILARY
                     DLASRCSVLFSPVFGSLEVRDLAEWILADRLPVRFQMQLHKYIWAPDMRGV"
     gene            1705..>2944
                     /locus_tag="VLL06_06025"
     CDS             1705..>2944
                     /locus_tag="VLL06_06025"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HSS30557.1"
                     /translation="MKIMQVTVQVGRAETVPAEVLVLTHCEGEGLAKQDAALLDRTLG
                     GALSNLVQSKEFEGKANEVLLYHTQGTVPAKRLALVGLGKRSEVTLEKIRQAMGSAAK
                     RARQTKVESFTVMLPTVTPSGMSSLEVTQAMVEGAILGSYQFTVYRSEAASGPDVSEM
                     KILIPRKSQLRQVTEGVRRGVAAAEATVFVRDLCNHPSNVLTPTRVADEAKTVAKAEG
                     ITIKILEQKEMERLGMGALLGVARGSQEPPKFIILEYNGAKKKDARPVVLVGKTITFD
                     TGGISLKPAENMEHMKADMTGGAEVLASIRAAARLKLPLRLISILPVAENMPGGRAMK
                     PGDIVTTLSGKTVEVQNTDAEGRLILADGLAYAMRYKPAALIDIATLTGACVVALGQF
                     AIGMFGTDQTLKEQVRKSGYK"
BASE COUNT          638 a          792 c          905 g          609 t
ORIGIN      
        1 gcttgcgcca cacgcccctc aaacgtgcgt gtctgccatt tcaccacttc ggcatatgaa
       61 attttcactt caacgacgag ggagccgcat tatagaagtg ggccaaaatc cttgtcaatc
      121 aacagacggt ccggtagaat cgggcggctt atgctcaccg ggttatccaa cgctgtgcag
      181 aatcaacaca ttctcacctc cgcactattt gacgatgtcg ctgaacgaca gggacagggt
      241 tccgtcgctg cgctgtttga cgtcgacaat acgctcctgc cgggggaggc cagtgaaata
      301 ggattcttcc ggtttttgtg gcggcgtggt ctagtgggat ggggcgagtt gactcgaagc
      361 gcggcctggc tggcggggca cgtgcctccg ttttcgctgc atgcacttcg agaacgaaaa
      421 gtgtacctga caggcaagtg tcccgcagac attgaatccc atgcgcgcga gttctgtcag
      481 gccgaaatga tcgacaagct gtctctacag ggtcgcacaa ggctcgacat gcatagacag
      541 gccggccacc agttgattct tgtgactggc gcgcctgagt ttttggtcgt accccttgca
      601 aagtacttgg gcatatctac agtattcgcg gcaaagccag aggagcggga ttcagtgtat
      661 accggtgcat tgattcctcc gttgccctat ggtcgtggca agcgtgagct gatcctcgct
      721 cacgctcagg acatgaatct cgacttgccc ggctcctacg cctatggcga cagcccaggt
      781 gaccgagaca ttcttgaatt ggtcgggcac cccttggtgg tcaacccgat ccggggcatg
      841 gcccgcacgg cacaagagcg aggttggcct gtcacactgt ggaagtagcg ccgtgaaccg
      901 tgaatcgcca actgatcgtt ttgtggtcat acagtatcca cacgtttcca gcaccgattt
      961 cacgattatt gcttcacgat tcacgtcttg aattatgcgc atcaccgaga tcttccatag
     1021 cattcaaggc gaatcgagct acgccgggcg gccctgtgtc tttgtacgac tgactggatg
     1081 cccactccgc tgcacctggt gcgataccga ctatgccttc tacggagggc gcgagtgctc
     1141 gatcgaagag gtcctggcgc aggtacaaaa ctatggatgc cgccttgtgg agttcacggg
     1201 aggggagccg cttgtccaac ccgagagttt cccgttcttg accagactgt gcgatgccgg
     1261 ctacacggtc ctccttgaaa ccagcggcgc cgttgatatt gcaccggtcg atccccgcac
     1321 ccacgtcgta ctggatgtaa agtgtccggg gagtgggatg accgatcgaa tgcactggcc
     1381 gaatcttgct cggttgactg ccaaagacga ggcgaaattt gtcctggccg accgagtcga
     1441 ttacgactgg gctcgcgaga ttcttgctcg atatgaccta gccagtcgct gttcggtact
     1501 gttcagcccg gtcttcggtt ccctcgaagt ccgtgatctt gcggagtgga tcctggccga
     1561 taggcttcct gtgcgattcc aaatgcagtt gcataaatat atatgggcgc ccgacatgcg
     1621 aggggtgtga tgagtcgcca tcgacgctcc gtgtgaactg aacgctgatg gctgaccgct
     1681 gacagcgtga acaaggagac acgaatgaag attatgcagg tcacggtaca ggttggacga
     1741 gcggagaccg tacccgccga ggtgttggtc ctcacgcatt gtgaaggaga aggattagcc
     1801 aaacaggatg cggccctgct cgatcgaacc ctcggaggcg cgttgagcaa tctggtgcag
     1861 tccaaggaat tcgaaggcaa ggcgaacgag gtcttgctgt accacacgca gggcacggtg
     1921 cctgcgaaac gactggcgct ggtcggcttg ggcaagagaa gcgaggtcac gcttgagaag
     1981 atccggcaag cgatgggctc ggcggcgaag cgcgcccggc aaacaaaggt cgaatcgttt
     2041 acggtgatgc ttccgacggt gacgcctagc ggaatgtcgt cgcttgaagt cacgcaagcg
     2101 atggttgaag gggcgatcct gggcagctat cagttcaccg tctatcggag tgaggcggcc
     2161 tcagggccag acgtgtcgga gatgaagatt ctcatcccga ggaaaagtca gttgcggcaa
     2221 gtcaccgaag gagtgcggcg cggcgtcgct gcggcagagg ctaccgtctt tgtccgagat
     2281 ctctgcaatc atccctctaa cgtcctaacc cccaccagag tcgccgacga agcgaagact
     2341 gtcgccaagg cggaagggat cacgatcaag attctcgagc agaaagaaat ggagcgcttg
     2401 gggatgggag cgctgctcgg cgtggcgcgc gggagccagg aaccgcccaa gttcatcatc
     2461 cttgaataca acggcgccaa gaaaaaggac gcacgcccgg tcgtgctggt cgggaagacg
     2521 atcaccttcg atacgggggg gatttcgctg aagcctgcag aaaatatgga acacatgaag
     2581 gccgatatga caggaggggc cgaagtcttg gcctcgatcc gcgcagcggc cagactcaag
     2641 ctcccattgc gtctcatcag tatcctgccc gtggcggaaa acatgccggg tggccgggcg
     2701 atgaagccgg gtgatattgt gacgacgctg tcggggaaga ctgttgaagt acagaacacg
     2761 gatgcagagg gccggctcat tctggcggat ggccttgcct atgcgatgcg ttataagccg
     2821 gcggccttga ttgacattgc cacgctcaca ggggcctgtg tcgtggcgct cggccagttt
     2881 gctatcggga tgttcggaac agaccagacg ctcaaagagc aggtccggaa gtcgggatac
     2941 aaag
//
LOCUS       DATIPF010000238         2831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_44198, whole genome shotgun sequence.
ACCESSION   DATIPF010000238 DATIPF010000000
VERSION     DATIPF010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44198"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /gene="metK"
                     /locus_tag="VLL06_06030"
     CDS             <1..730
                     /gene="metK"
                     /locus_tag="VLL06_06030"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HSS30558.1"
                     /translation="LTKRLAEVRKKNILPWVRPDGKSQVTVEYRNGKPVRIDTIVVST
                     QHSPNVTNKQIERDIMEKVIKPMMPKGLYDPTSVKHHINPTGRFVVGGPMGDTGLTGR
                     KIIVDTYGGHGSHGGGAFSGKDPTKVDRSASYMARYIAKNIVAAGLAQKCEVQLAYAI
                     GVADPVSVLVDTKGTEKVAVEHLDKLVRTHFPLTPRGIIDYLKLRRPIFRKTASYGHF
                     GRNEPEFTWEKTNKAKILRKDAGL"
     gene            829..2085
                     /gene="ahcY"
                     /locus_tag="VLL06_06035"
     CDS             829..2085
                     /gene="ahcY"
                     /locus_tag="VLL06_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HSS30559.1"
                     /translation="MEYDVKDIKLADQGKLKIEWAEATMPVLRLIKKRFQREKPFAGL
                     RVTACLHVTTETANLMKTLQAGGADVRLCASNPLSTQDEIAAALVKHDSIPTFAIKGE
                     DSKTYYQHIQSAIGHKPQITMDDGADVVSLIHSKRKDLLKHVIGGTEETTTGVIRLRS
                     MAEKKVLKFPVISVNDADTKHLFDNRYGTGQSTIDGIIRATNRLICGTSFVVAGYGWC
                     GRGIAMRAKGMGADVIVTEIDPVKAIEAVMDGFRVMPMEQAALIGDFFVTVTGNLKVI
                     RGEHFAAMKEGAIVCNSGHFNVELDIPALEKLSRKKRLIRPGVEQFTLKTGNRVSLLG
                     EGRLVNLATAEGHPSSVMDMSFANQALGAEYLVKNYQKLEKKVYPVPPVIDKEIARLK
                     LAGMGMKIDTLTKEQVKYLASWEMGT"
BASE COUNT          693 a          824 c          801 g          513 t
ORIGIN      
        1 gctcaccaaa cggctggctg aagtccggaa aaagaacatc ctgccttggg tccgtcccga
       61 tggaaagtcc caagtgaccg tggagtatcg gaacggaaaa ccggtgcgga tcgacaccat
      121 cgtcgtctcg acccaacaca gtcccaacgt gacgaacaag cagattgagc gcgacatcat
      181 ggagaaggtg atcaagccca tgatgccgaa aggactctac gacccgacga gcgtcaaaca
      241 ccacatcaat cccaccggcc gcttcgtggt cggcggtcca atgggcgaca ccggactgac
      301 gggccgtaaa atcatcgtgg acacctatgg cggccacggc agtcatggag gcggcgcctt
      361 ctccggcaag gatcccacaa aggtggaccg gtcggcatcc tacatggcac gctacatcgc
      421 gaagaacatt gtcgccgccg ggttggccca gaagtgcgaa gtgcagctcg cctatgcgat
      481 tggcgtagcc gatcccgtct cggtgctggt cgacacgaag ggtacggaga aagtggcagt
      541 cgagcatctg gataagctcg tacggacaca cttccctttg accccgcgcg gcatcatcga
      601 ctatctcaag ctccgccggc cgatcttcag aaagacagca tcatacggac acttcggtcg
      661 aaacgaaccg gagttcacct gggaaaagac caacaaggcc aaaatcttgc gcaaagacgc
      721 aggcctgtaa tagagaccga accgatcacc gggggacgga ttgaaaaatc cgcccccctt
      781 ttgctgacca cgcatgaccc gttactcatc actgtactgg aggtttctgt ggaatacgat
      841 gtgaaggata tcaagctggc agaccagggc aaactcaaga tcgaatgggc cgaggccacc
      901 atgccggtgc tgcggctcat caagaaacgg tttcagcgag aaaagccatt cgcagggctc
      961 cgcgtcacgg cttgtctcca tgtgacgacc gaaacagcca acctcatgaa aacacttcag
     1021 gccggcggag ccgatgtcag gctctgcgcc tccaatccgc tgagcacgca ggatgagatc
     1081 gcggcggcgc tcgtcaaaca cgacagcatc ccaaccttcg cgattaaggg cgaagacagc
     1141 aagacctact atcaacacat ccaatcggcc atcggccaca agccccagat cacgatggac
     1201 gacggggccg atgtcgtctc gctgatccat tccaagcgta aggatttgct caaacacgtg
     1261 atcggcggta cagaagaaac caccaccggc gtcatccgac tccgcagcat ggccgaaaag
     1321 aaagtactga agttcccggt gatctccgtc aacgacgccg acaccaagca cctgttcgac
     1381 aaccgctacg ggaccggaca aagcacaatc gatgggatca tccgcgcgac gaaccggttg
     1441 atctgcggca ccagcttcgt ggtcgccggc tatggctggt gcgggcgcgg gatcgcgatg
     1501 cgtgccaagg gcatgggggc cgacgtcatc gtcaccgaga tcgatccggt caaagcaatt
     1561 gaagcggtga tggacggatt tcgtgtcatg ccgatggaac aggccgcgct cattggagat
     1621 ttcttcgtca cggtgaccgg caacctcaag gtcatccgcg gtgagcactt cgccgcgatg
     1681 aaagaagggg ccatcgtctg caactcaggt cacttcaacg tcgagttgga cattcccgct
     1741 ttggaaaagt tgagccgcaa gaagcgcctc atcagacccg gcgtcgagca attcacgctg
     1801 aagaccggga atcgcgtcag cctactcggc gaagggcggc tcgtgaacct ggcgacggcc
     1861 gagggccatc catccagcgt catggacatg agcttcgcca atcaggcact cggtgctgag
     1921 tacctggtga agaattacca gaagctcgaa aagaaggtct atccggtccc gccggtcatc
     1981 gacaaagaaa tcgcgcgcct caagctcgcc ggcatgggga tgaagatcga tacgctgacg
     2041 aaggagcagg tgaagtatct ggcaagttgg gagatgggga catagcggtg ggcctgaggc
     2101 tcactgtcgc tcccaggctc ccggaatgac ccttccagcc tcaagcgaaa cgaaccttct
     2161 ggaattcctt cagctgcggt cgcttcgacg accgcagtcc ttcagtcagt tccagctttc
     2221 acttagcccg aggcttggtg gggccccatt cctccgccag ggtcgcgcca gaaagtctca
     2281 ggcccaccta gggaagaact ttaagatctg cgcaatgcgg accacgccgc accactcctg
     2341 ccagaaaatg gagagggagt aaagaaaagg ccaccggaaa cggtggcctt gttgtttgcg
     2401 ctcacgaaaa cgctgactga caccactcat cccacttaaa aagatggcga cgaatggttc
     2461 ccgccttgct tcccaccctc cgttttattc caagcgatag gccataggct atcagcccta
     2521 cgctctgtta atcccacgcc tctcccttca tctaacagac ctcctcacag agaggccgaa
     2581 tgtgccgact gagcgggcat gttttgccgg gtaaccggtg ctcagattca gctgaacaga
     2641 cagatcatgg gatttctgga agcttttgcc tctgtggagg gcggcacgaa agttgagaag
     2701 acttggcctg gtcttgaacc gtcaaggtgt ggatcgtgaa ttgaactgag gatacgaata
     2761 cgatgaacag tatgtcgggt ctatgggagt ttctgagtca ggatacggcg ttcttcggag
     2821 ggccccaaag c
//
LOCUS       DATIPF010000239         6391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1371489, whole genome shotgun sequence.
ACCESSION   DATIPF010000239 DATIPF010000000
VERSION     DATIPF010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371489"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VLL06_06040"
     CDS             <1..806
                     /locus_tag="VLL06_06040"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960674.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HSS30560.1"
                     /translation="MGVFFYCFRDREILMDWFDELCGARLTTSWYRIGGVERDFTPSL
                     LEKLKQFLDYFPPKIDEYVVFLEKNRIWVGRTKGVAIISAEDAVSFGLSGPTLRGSGV
                     DYDLRKAEPYSAYSKCEFSVPLGKNGDTYDRYWIRVQELYESVKIIRQCLEQMQDGPI
                     MADVPSVTFPPKQRVFTNLEAMIQQFKLFSQGFDAPPGEIYCGTEAHKGELGFYIVST
                     GGGKPYRLKIRAPSFIHMGAFDHMSRGYMISDAVTIFGTYDIVMGECDR"
     gene            892..3588
                     /locus_tag="VLL06_06045"
     CDS             892..3588
                     /locus_tag="VLL06_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSS30561.1"
                     /translation="MGLKPATNPDVEAAAIELSIDGKTVTAKNGVSLYDVISSTGKII
                     PAMCYHYTFDPFGSCGMCLVMQEGKKAPIRSCSAKATAGMIIRTEGDDLFQARKKAVE
                     KHLSVHPLDCPVCDADGHCELQDTAFQHGVTNLANAKQKFIPEDTRSPVLDFNMNRCI
                     ACAECINVCKDVLMIDALQFMKKGGFNQVVPKGDLALSCEFCGDCLAVCPVGAITNKF
                     SKYLYKPWQMKKTTTTCNYCGDGCQMYLETKDAEVVRVTSPLSWKNKWGDRADTAKGH
                     GGLCVRGRFGFEYIDSPARLKQPLLRKGNQLIETPWLETMHHVVDRLSDIQRKHGPDA
                     IAGLITARCTNEELYLFQKLMRAGFRTNQLDSSARYGHLNFVHASRHALEIGRSPNDW
                     EDLTKAKAVILIGSNITETNPLTAVRIKEAIRVYRAQVVVIDSAITNMAKLASHPVLI
                     KPGTEGLVIDGLVKATLELDLIDEDSVKKHPKAFEALKAALAHISLEQIAAQTGMSVE
                     SLKDTATIFAEAPRSIILCAEGIVRQADGYQNVLKLIDLAWVTGKLGQPGCGVNTVTE
                     EPNEQGAVDMGVAPEFLPGQASFHDQVVRDRVAKAWDVTLPAAGSGANLAEILKRCRS
                     GQIRALYVIGENPLATLPASMEVRAALDRLELLVVQDPFLTETARMAHAVLPACTFAE
                     KDGTFTNLEGRVLRVREAIDPIGESLPDWHIMTALANALGCQWAYESSNDIQAEIMKL
                     LPGYYNLGQPRKIVPTVDRYLSNGYVADVVARYRAPVPLDSKNMRPFALLMGQTLYHS
                     GKMSTRAPGLITIGPNTGRLRMNPQDMERLAVGEGTMVRLTSDRGSLQLMVQPDQSVA
                     PQTCFFPEHFNEPPVKDLMSVHVDPTTGVPSFKHTRVTIEKA"
     gene            3607..4194
                     /gene="nuoI"
                     /locus_tag="VLL06_06050"
     CDS             3607..4194
                     /gene="nuoI"
                     /locus_tag="VLL06_06050"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HSS30562.1"
                     /translation="MRVGTLIKQILEAALFDEIREAMKVTFKHMFHKPITFQYPREQR
                     TLPDTHRGALALLRYDDHQERCVGCDLCEAACPSRCIKVISSEDAERPLQRFASEFYI
                     DITKCVFCGYCVEACPVNALAMTKVYEFSTHDKRTLMFDKKRLYEIGERHLEDAKKYL
                     YAHNQETNVEESREYRYYFPQSVLKPTQPPPKHLG"
     gene            4199..4723
                     /locus_tag="VLL06_06055"
     CDS             4199..4723
                     /locus_tag="VLL06_06055"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HSS30563.1"
                     /translation="MVVVFFTYFALVSIAAGVLTVALRHPVHCGIALLALLVHVSGLF
                     VLLNAEFIWAMQVIAYAGAILVLYLFVLMLMNLKTDDRYFHSSYLYFLAPAVIGSLYM
                     LALLFRSPFGGAKGDATTAAVLQDGDTHAVGIKMFSEYLLQFEIVGVFLLGAVIGAVV
                     LAKTPKPISDKRDG"
     gene            4801..5103
                     /gene="nuoK"
                     /locus_tag="VLL06_06060"
     CDS             4801..5103
                     /gene="nuoK"
                     /locus_tag="VLL06_06060"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HSS30564.1"
                     /translation="MVPLSAYVAVSAVLFITGVLGVVIRRNFIIVLMSVEIMLNAANI
                     NLVAFSYYLESMAGQLVALFIIAIAAGEAAIGLAIIIVVFRGKISTNVDEMNLLKW"
     gene            5105..>6391
                     /gene="nuoL"
                     /locus_tag="VLL06_06065"
     CDS             5105..>6391
                     /gene="nuoL"
                     /locus_tag="VLL06_06065"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HSS30565.1"
                     /translation="MTDLLIKLIPLFPLLAVILNGFLGRRYSHEVAHRFAWGSVGLSF
                     LCTISVFIDILRTGTPHEVIAYQWIFGGDLTINLAYLVDPLTCIMLLVITGVGFLIHV
                     YSVGYMHGEDGFTRFFTYMNLFMVSMLLLVMGNNYAVLFIGWEGVGLCSYLLIGYYYD
                     KVSAAKAASKAFVVNRIGDAGFLLAIFLIFINFKTLDYTKVFAQVSQLSPEMATAIAL
                     CLLVGAVGKSAQLPLYTWLPDAMEGPTPVSALIHAATMVTAGVYMIVRNHAIFDASPT
                     AMTIVGIIGGLTALFAATIGLVQTDIKRVLAYSTVSQLGYMILGCGIGAYTAAIFHLM
                     THAFFKALLFLSAGSVIHALSGEQDIRKMGGLSAKIPWTYRLFLVGTVAIAGIPPLAG
                     FWSKDEILAHAFTHHHYLLYSLAAIGAFLTSFYMFRL"
BASE COUNT         1390 a         1785 c         1735 g         1481 t
ORIGIN      
        1 ccatgggcgt ttttttctat tgctttcggg atcgggaaat tctgatggat tggttcgacg
       61 aactctgcgg tgcacgcctc acgaccagtt ggtatcgcat tggcggtgtc gagcgggatt
      121 tcacgccctc gctgctcgag aaactgaaac aatttctgga ctactttccg ccaaaaatcg
      181 acgagtacgt ggtcttcctt gagaagaaca ggatctgggt tgggcgaacc aagggcgtgg
      241 ccatcatctc agcagaagat gctgtaagtt ttggtttgag tggaccgacg ctccgcggct
      301 caggtgtcga ctatgacctc cgtaaggccg agccttattc ggcctattcg aaatgtgaat
      361 tcagtgtacc gctcggcaaa aatggcgata cgtatgatcg gtactggatc cgcgttcagg
      421 aactctatga gagcgtaaaa attatcagac aatgcctgga gcagatgcag gatggcccca
      481 ttatggccga tgtgcccagc gtcacgtttc caccgaagca gcgggtcttt acgaatctgg
      541 aggccatgat ccagcagttc aagctcttct cgcagggctt cgacgctcct ccgggggaga
      601 tctattgcgg gaccgaagcg cacaaaggag aattgggatt ctacattgtc agcacgggtg
      661 gcgggaaacc ctaccgcttg aagattcgcg ccccctcgtt tattcacatg ggcgcattcg
      721 accatatgtc cagagggtat atgatctctg atgcagtcac gattttcggg acctacgaca
      781 ttgtgatggg ggagtgcgac agataaatgt gaaatgtgag atgtgaaacg ttaaaggtag
      841 cccggccatt gcggattata tttttcgttt cacggtttgc gaggaacaca tatgggatta
      901 aagccagcaa ccaatcctga cgtggaagca gcagccatcg aactgtcgat cgatgggaaa
      961 accgtcacgg caaagaacgg ggtctcgctc tacgatgtca tctcgagcac gggcaagatc
     1021 atcccggcca tgtgctacca ctataccttc gatcccttcg gctcctgcgg tatgtgtttg
     1081 gtcatgcagg aaggtaagaa agcgcctatc cgttcctgct ccgccaaagc gacggccggc
     1141 atgatcatca gaaccgaggg ggatgatctc ttccaagccc ggaagaaagc cgtggaaaaa
     1201 catctctcgg tccatccgct cgactgcccg gtctgcgacg ccgacggtca ttgcgaactg
     1261 caggacacgg ccttccagca cggggtcacg aacctggcca acgcgaaaca gaaatttatt
     1321 cctgaagata cgcgcagccc ggtgctcgac tttaatatga accgctgcat tgcctgcgcg
     1381 gaatgcatca acgtgtgtaa agacgtgttg atgattgatg cgctccagtt catgaagaag
     1441 ggcggattca atcaagtggt gccgaaggga gatctcgcac tgtcctgcga gttttgtgga
     1501 gactgtctcg ccgtctgccc agtcggcgcg atcacaaata agttctctaa atatctctat
     1561 aagccgtggc agatgaagaa aaccacgacg acgtgtaact actgcgggga cggctgccag
     1621 atgtacctcg agaccaagga tgctgaagtg gtccgcgtga cctcgccgtt gtcgtggaag
     1681 aacaaatggg gcgaccgggc cgacacggcc aaggggcatg gtgggctttg tgtgcgaggg
     1741 cgctttggct tcgagtacat cgatagcccc gcacgcttga aacaaccgct ccttcgcaaa
     1801 ggcaatcagc tcatcgaaac cccctggctc gaaaccatgc accacgtggt tgatcggctt
     1861 tccgacatcc agcgcaagca tggacccgac gcaattgccg gtctcatcac ggcccgctgc
     1921 accaacgaag agctctatct ttttcagaaa ctcatgcgag ccgggttccg caccaaccaa
     1981 ctggacagca gcgcgcgcta cggccatctc aacttcgtgc atgcctctcg acatgccttg
     2041 gagattgggc ggagtccaaa tgattgggaa gatctgacga aggccaaggc tgtgatactc
     2101 attggttcca acatcacgga gaccaaccca ctcacggccg ttcgcatcaa agaagcgatt
     2161 cgcgtctata gagcccaagt ggtggtcatc gattccgcca tcacgaacat ggccaagctc
     2221 gcatcccatc cggtcctgat caaaccaggt acagaagggc ttgtcattga cggacttgtg
     2281 aaggccaccc tggagttgga cttgatcgac gaggacagtg tcaagaaaca ccctaaagct
     2341 ttcgaagccc tgaaggctgc cctggcccac atatcgttgg agcagatcgc tgcacaaaca
     2401 gggatgtcgg tcgaatcgtt gaaagatacc gcaacaatct ttgccgaggc accccgttcc
     2461 atcattctct gtgcggaggg catcgttcgc caggctgacg ggtatcagaa cgtgttaaaa
     2521 ctgatagacc tcgcctgggt cacgggcaag ttgggccagc ctggctgcgg ggtgaatacg
     2581 gttacagaag aaccgaacga gcagggcgcc gtagacatgg gcgtggcgcc tgaattcctc
     2641 cctggccaag cctcgtttca cgatcaggtg gtacgcgacc gtgtggccaa ggcgtgggac
     2701 gtcacgcttc cagcggctgg gtccggtgcg aatctcgccg agattctcaa gcgctgcagg
     2761 agcggccaga ttcgtgctct ctatgtgatc ggagaaaatc cgctcgccac gttacccgca
     2821 tccatggaag ttcgcgccgc gctggatcga ctggaattac ttgtcgtcca ggatccattc
     2881 ctgaccgaaa ctgcccgcat ggcccatgcc gtactcccgg cttgtacctt tgcggaaaaa
     2941 gacggcacct tcaccaatct ggaaggtcgg gtcctacggg tccgtgaagc tatcgatcca
     3001 atcggggaga gtttgccgga ttggcacatc atgacggcac tggccaatgc actgggctgt
     3061 caatgggcgt atgaatcctc aaacgatatt caggccgaga tcatgaagtt gcttcctggg
     3121 tactacaact taggacagcc acgcaaaatt gtgccgactg tggaccgcta tctctcaaac
     3181 ggttatgtcg ctgatgtggt ggcgcgctat cgagcccccg tgccacttga ttccaaaaat
     3241 atgcgaccct tcgcgttgct gatgggacaa accttgtacc attcaggtaa gatgtccacc
     3301 agggctcccg gacttatcac gatcgggccc aacactgggc ggctcaggat gaaccctcaa
     3361 gatatggaac ggctcgcagt gggcgagggg acgatggtgc gcctcacgtc ggaccgcggc
     3421 tcgctgcaat tgatggtgca gcccgatcaa tccgttgctc cgcagacttg ctttttcccg
     3481 gagcatttta atgaaccacc ggtgaaggat ttgatgtcgg tccatgttga tccgaccaca
     3541 ggggttccct cgttcaagca cacccgcgtg acgatcgaaa aagcatagcg gaaacaggac
     3601 acccatatgc gtgttggcac actcatcaag caaatcctcg aggctgccct gttcgatgag
     3661 atccgggagg ccatgaaggt cacgttcaag cacatgttcc acaaacccat cacctttcag
     3721 tatccacggg aacaacgcac ccttcccgac acccatcggg gggcactcgc gctcttacgc
     3781 tacgacgatc accaggaacg ttgtgtcggc tgcgacctct gtgaggccgc ctgtccatcg
     3841 cgttgtatca aggtcatcag ttcggaggat gcggaacgtc cactgcaacg attcgccagc
     3901 gagttttaca tcgacattac gaagtgcgtc ttttgcgggt actgtgtcga ggcctgtcct
     3961 gtcaacgcgc tggcgatgac aaaggtgtat gagttctcga cccatgataa acgcaccttg
     4021 atgtttgaca agaagcgatt gtacgaaatc ggcgagcgac atcttgagga tgcaaaaaaa
     4081 tacttatacg cgcataatca ggaaacgaac gttgaggaaa gcagggaata tcggtattac
     4141 ttcccgcaat cggtactgaa gccgactcaa ccgccaccca agcatctggg ctgagtcgat
     4201 ggttgtcgtc ttttttacct atttcgctct cgtcagtatt gctgcaggcg tcttgacggt
     4261 cgcgctcaga caccccgtgc attgcggcat tgcactcctg gccctgttgg tccatgtctc
     4321 cggactcttc gtcctcctca acgccgagtt tatctgggcc atgcaggtca tcgcctacgc
     4381 cggtgcgatc ctcgtcttgt acctcttcgt cctgatgctc atgaacttga agaccgacga
     4441 tcgatatttc cattcctcat acctgtactt tctggctcca gcagtcatcg gatcattgta
     4501 tatgttggcc ctcctgttcc gttcaccctt cgggggcgca aagggcgacg cgactactgc
     4561 ggcagtactc caggacggcg atacccacgc ggtcgggatc aagatgttca gtgagtatct
     4621 gttgcagttc gaaatcgtcg gagtgtttct gttgggtgcc gtcatcggag cggtcgtgct
     4681 ggcaaaaacg ccgaagccga tctctgacaa acgtgacggg tgagtgttga attgactacg
     4741 gcaacatgcc gcagctcttc aaccgttcag cactaagctt caacataatc attgggatgt
     4801 atggttcctc tcagcgcata tgtcgccgtc agcgccgtgc tctttatcac cggagtgctc
     4861 ggcgtcgtaa tcagacgaaa ctttatcatc gtgttgatgt cggtcgagat catgttgaat
     4921 gcggcgaata tcaaccttgt agccttctcc tattacctgg aatcaatggc aggtcaactg
     4981 gttgccctgt tcatcatcgc cattgcggcc ggagaagcag ccatagggct cgcgatcatc
     5041 atcgtcgtgt tccgtggaaa gatctccacg aacgtggacg agatgaacct cttaaaatgg
     5101 taacgtgacc gatctactca tcaaactcat tccgctgttc cccctgctgg ctgtgatcct
     5161 gaacgggttt ctgggcaggc gatattccca cgaggtcgcg catcgattcg cctggggatc
     5221 ggtggggttg tcatttctct gcacgattag tgtgtttatc gatattctac ggaccggcac
     5281 ccctcatgaa gtcatcgcct atcaatggat cttcggcggc gacctcacca tcaatctggc
     5341 ctatctcgtc gatccgctca cgtgcatcat gctgctcgtc atcacggggg tcgggttcct
     5401 gattcacgtc tactccgtcg gctacatgca cggggaggac gggttcacgc gatttttcac
     5461 ctacatgaac cttttcatgg tctccatgtt gctcctcgtc atggggaaca actatgccgt
     5521 actctttatt ggctgggagg gggtagggct ctgttcctat ctcttgatcg gctattacta
     5581 tgacaaggtt tccgccgcga aggccgcctc gaaagccttt gtggtgaacc gcatcggcga
     5641 cgcaggcttc ctgttggcca ttttcctcat cttcattaat ttcaagacgc tcgactatac
     5701 gaaagtcttt gcccaggtga gccagctctc gccggagatg gcgacagcga ttgcgctctg
     5761 cctgctcgtc ggagccgtcg gcaaatcggc ccagctccct ctctacacgt ggctcccgga
     5821 tgcgatggag ggtcccacac cggtcagcgc gctcatccat gcagcgacga tggtgacggc
     5881 cggcgtgtac atgatcgtgc ggaaccatgc catcttcgat gcatcgccga cggccatgac
     5941 gatcgtcggc atcatcggcg gcctcaccgc cctgtttgcc gccaccatcg gcctcgtgca
     6001 gacggatatc aaacgcgtct tggcctattc gacggtgagc cagctcgggt acatgattct
     6061 cgggtgtgga ataggggcct ataccgccgc catctttcac cttatgaccc atgccttctt
     6121 caaagccctc ttgttccttt cggccggatc ggtgattcat gccctctcgg gtgagcagga
     6181 cattcgaaag atgggcggtc tcagtgcgaa aatcccatgg acctatcgac tctttctggt
     6241 cggtacggtc gccattgcgg gcattccacc gttggcaggc ttctggagca aagatgaaat
     6301 cctggcccat gccttcacgc atcaccacta tctgctgtat agcttggctg ccatcggggc
     6361 cttcttgacc tccttctata tgttccgcct g
//
LOCUS       DATIPF010000240         7798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1018968, whole genome shotgun sequence.
ACCESSION   DATIPF010000240 DATIPF010000000
VERSION     DATIPF010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7798
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018968"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="VLL06_06070"
     CDS             complement(<1..1148)
                     /locus_tag="VLL06_06070"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSS30566.1"
                     /translation="MDQAIVEVRDQEGTVVVQGVTNQAGEFSVSVPQEGTYSVSAIHE
                     TYRRECVVVTVGTDKPVSVTLTLALTQDIALEIRSPLPAIQYKASSETYSVSRKDIEV
                     LPLGNNNTVAQVLQTVPSVVYGALGQTHIRQDHSNQQFRIDGVPIPEGVSSTFTDVIS
                     PRMWERADIILGGMEAQYGNKTALVVDITSKSGTRPSFGSAQMFGGSNQTVNPSFEYG
                     GTIGEKVRFYALNSYTSTNRGIEPPTAGHSVFHDQSERNQTYLRGDYQHNNRNSLSWV
                     FLNAVAKYQIPTLPGLAVNPDVLPLLQAQAPTFSPAASQAVNQNQKENSQYTHLVWRH
                     DVDAEQSFQLAGFLRNSSASFTTDPFNTLAYTPDQQTANQKRKAYSLGT"
     gene            complement(1387..1719)
                     /locus_tag="VLL06_06075"
     CDS             complement(1387..1719)
                     /locus_tag="VLL06_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30567.1"
                     /translation="MMLPASKLRLPSVIRGTVAIGLVLLLLAIAPFAVAQDVHHELAA
                     ADHDGHEHSDTDLCQWVQHHTAGSLDDGSPLLTAQKLVDLLDLPVDSVLLSVELSTPG
                     PSRAPPCK"
     gene            1920..2978
                     /locus_tag="VLL06_06080"
     CDS             1920..2978
                     /locus_tag="VLL06_06080"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HSS30568.1"
                     /translation="MQRPLDNQHIIDIKPLPSPRTIKSKLPITDQAAALVVETRDAVR
                     LILHGQDRERLLVIVGPCSIHDPEAAYEYADRLKPVADALRDRLLIVMRTYFEKPRTT
                     IGWKGLINDPHLDGTCDIATGMELARTILLNINKRGVPCAAEALDPVSPQYIADLWSW
                     VAIGARTTESQPHREMASGLSMPVGFKNGTEGNLQVAWNAMVAAKQPHHFLGVNADGL
                     TSIIKTTGNPDRHIVLRGGGGKTNYEAEDVARAEAVVASEGIVRPIMIDCSHDNSSKD
                     HRRQGLVAREVLRQFREGRHSIMGLMLESNLNPGKQTWKQGISLAHGVSITDACLGWK
                     ETEALLSELADMIVTKPI"
     gene            3073..3852
                     /locus_tag="VLL06_06085"
     CDS             3073..3852
                     /locus_tag="VLL06_06085"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HSS30569.1"
                     /translation="MLFDTHTHLDDARYNEDREAMIARAREAGVEAFVTIGCDLATSR
                     AAVALAEQHPSIYASIGVHPHEVKHILDSWYDNFRRLAKNKKVVAYGEIGLDYHYNHS
                     SPKEQRERFREQIQLARELTLPVIIHTREAQEDTIAILKEERASEIGGVFHCFSGDAW
                     LAKDALDLGFYLSFSGILTFQNATMLRDIAKTVPLDRLLIETDCPYLTPMPHRGRRNE
                     PAYVSFVAQKLAALHADEPGMSADTIGHITTQNAKHLFKIA"
     gene            complement(3839..4372)
                     /locus_tag="VLL06_06090"
     CDS             complement(3839..4372)
                     /locus_tag="VLL06_06090"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HSS30570.1"
                     /translation="MARHLIVDGYNLLAQTGRIGGGTSLHSEMAREAMLRDLATYRQR
                     KSHAITVVFDGWQQGWGTEQREHRLGLEIIFSRRGEKADQVIQRLAADFGSSCAVVSS
                     DREIGDFVRAQGAFVMKAQEFVEKLREGSNSTRVSAHKELDTGEDLHPKRGSEKRGNP
                     RKLPKALRQRSRQLKRF"
     gene            4490..5773
                     /locus_tag="VLL06_06095"
     CDS             4490..5773
                     /locus_tag="VLL06_06095"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HSS30571.1"
                     /translation="MPSALWRIFTLAFLVGLFDLSGHALGLFHTGRALAATSPSHEEA
                     AAKSKRSRTSPIRMAPTALGLTTIQNLRTASSPGMTRLVLDLDAKARPSKHPQLRAEG
                     VTIEIPNATLSSSAKTKLAAGKIAKPFVITQSSERSVEVSLPAGSFQSYRLLTLANPY
                     RLVIDVVRPGEPLSAPSVESTPSLELPLPTPPQPVQPRAKSYTTIVIDPGHGGRDPGA
                     RGQRGTEEKDITLKVALKLRDLLRRQPGIRVLMTRERDQFIELEDRAKFANGQEADLF
                     VSIHVNSHPQRSVKGIEIYHFGEAKDQRALEVAARENGTPLSSTGVGWEYLVADLLTA
                     KKIEASLELAWTAKEAMVTNLNGHYALVDHGVKTAPFYVLRFTSMPSILAEIAYISNS
                     VEEDLLRTSHFSTRVAEALMDGINTFLASSKLSSR"
     gene            5802..6539
                     /gene="truA"
                     /locus_tag="VLL06_06100"
     CDS             5802..6539
                     /gene="truA"
                     /locus_tag="VLL06_06100"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HSS30572.1"
                     /translation="MPTVKLVLEYDGTRYAGWQRQPDHPTIQEAIERAIHQVSHATVS
                     IVGAGRTDSGVHARGQVASFCTDLDWPASNWMRALNAVLPKDIAVCASALMDDRFHAQ
                     HDARGKLYTYRILNRPARPTIDRAFVWHIYKPLNDAAMQQAAARLIGSQDFSSFEGSL
                     TDNTNPICHLERLAVIRHADQIHIEAYADRFLKHMVRAIVGTVVEVGLSKRTPGSLAE
                     ILQARTRSAAGQTAPPHGLCLMRVDYE"
     gene            6800..7111
                     /locus_tag="VLL06_06105"
     CDS             6800..7111
                     /locus_tag="VLL06_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HSS30573.1"
                     /translation="MTKTGSIVVVFLFVSGLCIVPIAIAAPTTQSKMAACNTQANDKG
                     FGEGKGEERTAFMKECLSEKPAKAGGTQQNKMKSCNKEAGAKSLKGEERKKFMSTCLS
                     S"
     gene            complement(7248..>7798)
                     /gene="epsC"
                     /locus_tag="VLL06_06110"
     CDS             complement(7248..>7798)
                     /gene="epsC"
                     /locus_tag="VLL06_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HSS30574.1"
                     /translation="VLAYRVSHWLKTHRVPFLPRFISQAARILTGIEIHPSAIIGSGF
                     FIDHGMGVVIGETAEIGNYVTLFQGVTLGGTGKERGKRHPTLGSHVVVGAGAKILGGI
                     TVGDNVKIGANSVVLKNVSANSTVIGVPARVIKTQGERLPDATMDHINLPDPISDRLL
                     AFEQELIELRKKLESQDSPRLF"
BASE COUNT         1812 a         2305 c         2082 g         1599 t
ORIGIN      
        1 gtcccgagtg agtaggcctt gcgtttctga ttggccgtct gctgatcggg cgtgtaggcg
       61 agtgtgttga acgggtcggt cgtgaagctc gcagacgagt tccgcagaaa cccggcgagc
      121 tggaaagact gttcggcgtc cacgtcatgc cgccagacca gatgggtgta ttgagaattt
      181 tccttctggt tctggttcac cgcctgggag gctgcgggtg aaaaggtcgg ggcctgcgcc
      241 tgaaggaggg gcagtacatc tgggttcacg gccaagccgg ggagcgtcgg aatctgatac
      301 ttcgccacgg cattcaagaa cacccagctc agactgttgc gattgttgtg ctgatagtcc
      361 cctcgcagat aggtttgatt gcgctcgctc tgatcgtgaa agaccgaatg cccggccgtc
      421 ggcggctcga tgccccggtt ggtcgacgta tagctgttga gggcataaaa gcggaccttc
      481 tcaccaatcg tgcccccgta ttcgaacgac ggattcaccg tctggttcga gccgccaaac
      541 atttgggccg agccgaacga gggacgcgtt ccgctcttgc tggtaatatc gaccacgagc
      601 gccgtcttgt tgccgtactg agcctccatg ccgccaagga taatatccgc gcgctcccac
      661 atgcgaggcg aaatcacgtc cgtgaaggtc gaacttaccc cttccggaat cggcacccca
      721 tcgatccgga actgctgatt ggagtgatcc tgacggatat gggtttgccc caaggcccca
      781 tacaccacac tcggaaccgt ctgtaggacc tgcgcgacag tgttattgtt gccgagaggg
      841 agcacttcga tatccttccg gctcacggaa tacgtttcgc tcgaggcctt gtattggatc
      901 gcgggtagcg gcgatctaat ttcgagcgcg atgtcctgcg tcagtgctaa cgtcagggtg
      961 accgataccg gcttgtccgt ccctaccgtt accaccacac attcacggcg gtaggtctca
     1021 tggatggcgc tcaccgaata ggtcccctcc tgtggcacgc tgacggaaaa ttcgccagcc
     1081 tggttcgtca cgccttgaac gaccacggta ccttcctgat cgcgcacctc cacgatggct
     1141 tgatccaccc gtcggagatc ctgattctgg acagatccgg tgatgaaatt gggctcggcc
     1201 gcaacagcat gaccctgata aagaacactc agacagagga cacagagaca taccgaacga
     1261 acattacggt ggccccatat attgggtcgc atgaaatcct cctacaatca cgcattaaaa
     1321 gacgtcacag tcggcaggca gtccgacgac gaccgtgcca gaatggtgag attcgaaata
     1381 acgtgactat ttgcagggag gagcgcggga gggaccaggc gttgagagtt cgacagagag
     1441 cagaacggag tccactggaa ggtccaacag atcgacaagt ttctgtgcgg tgaggagagg
     1501 ggagccatca tcgagagagc cggccgtgtg gtgctgcacc cactggcaga ggtcggtatc
     1561 ggaatgttcg tgcccatcat ggtcagccgc cgccaactcg tgatggacat cctgtgcgac
     1621 ggcgaagggg gcaatggcga gcaaaagaag gaccaagccg atcgccactg tgccgcgaat
     1681 aacggagggc agacgcaatt tggaagcggg tagcatcatt gagggcgagc taagtaacat
     1741 aggtttcagg gcttgtcaaa cagcggggtt atgacattct tcccagcacc ttcagacctt
     1801 cgactgttcc taatgtggat atttccttta atatacgcta tatcttaagc ggctaagtcc
     1861 gtttcgtacc catgtagggt atgccaacgt ggatgatttc cgggcaaaca ggcaggacca
     1921 tgcagagacc tctcgacaat cagcacatca ttgatatcaa gccgctgccc tcgccgcgaa
     1981 cgatcaagtc aaaactgccg attaccgacc aagccgccgc actggttgtg gaaaccaggg
     2041 atgccgtccg cctcattttg cacggtcaag accgtgagcg cctactcgtg atcgtcggtc
     2101 cctgttcgat ccatgatccc gaggccgcct acgagtatgc cgaccgattg aaacccgtgg
     2161 ccgatgcctt gcgcgaccgg ctactgatcg tcatgcgcac ctactttgaa aaaccccgca
     2221 ccacgattgg ctggaaaggc ctcatcaacg atccgcattt agacggcact tgcgacatcg
     2281 ccaccggaat ggaactcgcg agaacgattc tcctcaatat caacaaacgc ggagtcccct
     2341 gtgcggctga agccttagat ccggtatcac cccaatatat cgcagatctc tggagttggg
     2401 tcgccatcgg cgcccgcaca accgaaagcc agccgcaccg ggaaatggcc agcgggctgt
     2461 ccatgccggt cggcttcaag aacggcacgg aaggcaatct tcaggtggca tggaatgcga
     2521 tggttgccgc gaagcagcct caccattttt tgggcgtcaa cgccgatggg ctcacctcca
     2581 tcatcaagac gaccggcaac cccgaccggc atatcgtctt gcggggcggc ggcggcaaaa
     2641 ccaattatga ggccgaagac gtggcgcgcg cagaggccgt tgtcgcgagc gagggtatcg
     2701 ttcgtcctat catgatcgat tgttcgcatg acaactcgag taaagaccat cggcgccagg
     2761 ggttggtcgc gcgcgaagtc ctccgccaat tccgcgaagg ccgccattcc atcatgggcc
     2821 tgatgctcga aagcaatctc aaccccggca aacaaacctg gaagcagggc atctccctgg
     2881 cccatggcgt ctccatcacc gacgcctgcc tcggctggaa agaaaccgaa gctctgctct
     2941 cagaactggc cgacatgatt gtaaccaaac ccatctgaac ggttggtagg tttgtttcgt
     3001 ttatttggtt tatctggtta aattcgttca accaaataga ccagatagac cagaccaacc
     3061 agacgaaccc acatgctttt cgatacgcat acccatctgg atgatgcccg ttacaatgag
     3121 gatcgcgagg cgatgattgc ccgcgcccgg gaagccggtg tcgaagcctt cgtgaccatc
     3181 ggctgcgatc tagcgaccag ccgagcggcc gtcgcactgg cggagcaaca cccgtcaatc
     3241 tatgcctcca tcggcgtcca tccacatgaa gtgaaacata tcctcgatag ctggtacgac
     3301 aactttcgcc ggctggcgaa gaataagaag gtcgtcgcct acggcgagat cggcctcgac
     3361 taccactaca atcactcatc gccgaaagaa cagcgcgagc gattccgcga gcagatccag
     3421 ctcgcacgcg agctgacact tccagtgatc attcacaccc gggaagctca agaagacaca
     3481 attgcaattt taaaagagga acgcgcgtca gaaatcggtg gggtatttca ctgcttctca
     3541 ggggatgcgt ggctggcgaa agatgcgctg gacttagggt tctatctctc gttctcaggg
     3601 attctcacgt ttcagaacgc gacgatgctg cgggacatcg caaagactgt tccactcgat
     3661 cgactactga tcgaaacaga ctgcccatac ctgacgccta tgcctcaccg aggcaggcgt
     3721 aacgaaccag cctacgtgtc cttcgtcgca caaaaactgg cggcacttca cgccgatgaa
     3781 ccaggcatgt cggccgacac catcggacac atcacgaccc agaacgcgaa acacctgttc
     3841 aaaatcgctt gagctgtcgg ctgcgctgcc gcagcgcctt gggtaatttc cgcggattcc
     3901 cgcgtttctc agaccctcgt ttcgggtgaa gatcctcccc cgtatccagt tctttgtgtg
     3961 ccgatacacg ggtcgagttc gacccctccc gcagtttctc aacaaactcc tgcgctttca
     4021 tcacaaaggc tccctgtgcc ctcacaaagt cgccgatctc acgatcggaa ctcaccaccg
     4081 cacaggagga tccgaaatcg gcagccagac gttgaatgac ctgatcggcc ttttctcccc
     4141 gtcgagaaaa gatgatctca agaccgagtc gatgctcccg ctgttccgta ccccatccct
     4201 gttgccaccc atcaaagacg accgtgatcg catgggattt ccgttgacga taggtcgcca
     4261 agtcgcgcaa cattgcctcg cgcgccatct cggaatgcag gctcgtccct ccgccaatac
     4321 ggcctgtctg agccaagaga ttgtacccgt cgacgatcag gtgtcgtgcc atgactgaaa
     4381 ctaccgtccg aaccgcatcg tgtcaatcgg ttccattgcg ccttcctctt gacaatgaca
     4441 cacacatttg agaaaaaact ctatatccca tcacacgcaa cgaacaccca tgccatcagc
     4501 cctgtggcgt atctttaccc tcgccttctt ggtcggactc ttcgacctct ctgggcatgc
     4561 cctcggtctg ttccacaccg gccgggcctt ggcggcaaca agcccctccc atgaggaggc
     4621 tgcggccaag tccaaacggt cccgcacctc ccctatacgt atggctccca ctgcactcgg
     4681 cctgacgacg attcagaacc tccggacagc cagctcgcca gggatgacga ggcttgtgct
     4741 cgatctcgat gcgaaagccc gccccagcaa acacccgcaa ctccgggctg aaggggtgac
     4801 catcgaaatt cccaatgcta ctttaagttc atccgccaag acaaaacttg ccgccggtaa
     4861 aattgccaag ccctttgtca ttacccagtc gtctgaacga tccgttgaag tctccctccc
     4921 tgccggctca tttcaaagct acaggttgct caccttggcg aatccctatc ggcttgtcat
     4981 cgatgtggtg cgtcccggtg aacctctgag tgctccatcc gttgagtcaa caccaagcct
     5041 tgagcttcca ttacccactc cccctcagcc tgtccagccc cgagccaaat catatacgac
     5101 cattgtgatc gatccagggc atgggggaag agaccctggc gcacgaggac aacgtgggac
     5161 agaagaaaaa gacattacac tgaaagtcgc attgaaactg cgcgatctct tgaggagaca
     5221 gccgggaatc cgcgtgctca tgacccgtga gagggaccaa ttcatcgaat tggaagaccg
     5281 ggcgaagttt gccaacgggc aggaggcgga tctttttgtg tcgatccatg tgaattcaca
     5341 cccacaacgt tccgtaaagg gaattgaaat ctatcacttc ggagaggcca aggatcaacg
     5401 ggcgctggaa gtggcggccc gtgaaaatgg aaccccgctc agcagcaccg gcgtgggatg
     5461 ggaatatctc gtcgccgatc tcctaacagc gaaaaagatt gaagcatctc tcgaactcgc
     5521 ctggaccgcc aaggaggcca tggtgaccaa tctgaacggc cactatgcat tggtggacca
     5581 tggagtgaag actgcaccct tctatgtgct ccgcttcacc agcatgccca gcatcctggc
     5641 tgaaatcgcc tatatttcga actctgtcga agaagatctg ctccggacca gccacttcag
     5701 cacgcgcgta gccgaagcac tcatggacgg catcaacacc ttcctggcct cttcgaaact
     5761 ttccagtcga tgacgtccgc acccttccat ccccaacgtc gatgcccacg gttaagctag
     5821 tcttggaata cgatgggacc cgttacgccg gttggcagcg ccagcccgat catcccacga
     5881 tccaagaagc catcgagcgc gccatccacc aggtcagcca tgccacggta tccatcgtcg
     5941 gcgcaggccg tacagattct ggcgtccatg cgcgtggaca agtggcaagc ttttgtacgg
     6001 atctggactg gccggcgtcc aactggatgc gggcactgaa tgccgtgctg ccgaaagata
     6061 ttgccgtctg tgcctccgcc ctcatggacg accgctttca tgcccaacac gatgcgcgag
     6121 gaaaactcta cacctatcga atcttgaatc gcccggcccg ccctacgatc gaccgcgcat
     6181 tcgtgtggca catctacaag ccgctgaatg acgccgccat gcaacaggca gcagcacggc
     6241 tgattggatc acaggatttt tcctcctttg aaggaagtct cacggacaac accaatccca
     6301 tctgccatct cgagcggctc gccgtcatcc gccacgcaga ccagattcac atagaggcct
     6361 acgccgaccg ctttctgaag catatggtcc gcgcgatcgt tggaacagtg gtcgaagtgg
     6421 ggctgagtaa acggaccccc ggcagtctgg cagagattct acaggcccgg actcgttctg
     6481 cggcaggcca gaccgccccc cctcatggac tctgtctcat gcgcgtcgat tatgaataga
     6541 aggctgcagt agaagtccgt caggtgtgtc tcatatgtac ggctgaacaa aacaaactga
     6601 tggacgaaat gcgtcggatg aaccttcctc gctgtctcac tcaacgctct cagctgtttc
     6661 gaccttcgac ttccagataa tccaatcatt attactgggt ttgcagatag gctgcctaat
     6721 attattagag ataattttaa taaggtatgc tatcgtgcgc aagctcttca tacctcactc
     6781 ttaataaaag gagacggata tgacgaaaac gggcagcatc gtagtagtat tcctctttgt
     6841 gtccggattg tgcatcgtgc cgatcgccat cgcggcgccc acaacgcaaa gcaagatggc
     6901 tgcctgcaat acccaagcga acgacaaggg attcggtgaa gggaagggcg aggagcgaac
     6961 ggcattcatg aaagaatgcc tctccgaaaa accggcgaag gcgggtggga ctcagcagaa
     7021 taagatgaaa tcttgcaaca aagaagcagg tgcaaaaagc ctgaagggcg aggagcgtaa
     7081 aaagtttatg agtacctgcc tgtctagcta actcataatc gcattatccg acgtggtcca
     7141 cctgggccac gtcagctttc ctcggcaccg accgtttcag aaacgcgaca ggattctcct
     7201 cagtggcctg aggaaacatg gtgacagcta tgggagggaa tccgaggcta gaacaggcgg
     7261 ggggagtcct gactctccag tttcttgcgc agctcgatca actcctgctc gaatgccaag
     7321 agacggtcac tgatggggtc tggcaggttg atgtggtcca tggtggcatc gggaagccgc
     7381 tcgccttgag ttttaatcac acgggccggc actccaatga cggtggaatt ggcagagacg
     7441 ttcttgagca caaccgaatt ggcaccgatc ttgacattat cgccgaccgt gatcccgccg
     7501 agaatcttcg ctccggcccc gacgaccacg tgactgccaa gcgttggatg ccgtttgccc
     7561 cgctccttgc ccgtcccacc aagcgtcacg ccttgaaaga gggtcacgta atttccgatc
     7621 tctgccgttt caccgatcac cacacccatt ccatggtcga tgaagaaccc ggagccgatt
     7681 atagccgagg gatggatttc gatccctgtc agtatgcgag cagcctggga aatgaagcgg
     7741 ggaagaaacg ggacacgatg ggtcttgagc caatgggata cccggtatgc caatacgg
//
LOCUS       DATIPF010000241         5459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1722420, whole genome shotgun sequence.
ACCESSION   DATIPF010000241 DATIPF010000000
VERSION     DATIPF010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1722420"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1621
                     /gene="rpoN"
                     /locus_tag="VLL06_06115"
     CDS             110..1621
                     /gene="rpoN"
                     /locus_tag="VLL06_06115"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HSS30575.1"
                     /translation="MKLRLDLRLSQKLIMTPQLQQAIKLLQLSRLELQQSLTQHLMEN
                     PLLEDLPAEAEDSEATSAEEKAEDAAASADSEPSNVEESTPEERDTPDEASAAGWEEY
                     FGSDRRIGGSESQSSSQDEFPSYEQTMAKATSLEDHLLWQLSFSGLSDREKGIGRLII
                     GNLDDDGYLRMSLAEMVAGTDFQEQEAESVLKDIQSFDPTGVAARDLVECLLLQIGHL
                     RTSTMGSLGARPGALKGSIVEAIVQHHLKDLEKKQYARIAKALDVTVDEVFQATKVIE
                     ALEPKPGRPYTNTQNYVIVPDVFVVKNEGEWVVLLNDDGLPRMRISPYYKQLMGAGES
                     GSAETKAYLDEKLRAAQWVIRSIEQRNKTIVKVVSSIVKFQEQFFEKGVQHLKPLVLK
                     QVAEDIGMHESTISRVTANKYMYCPQGMLELKFFFNAGLQRADQPSDMMSSVTVREMI
                     RKMVAEEDASHPLKDEEIAARLLTQQVVIARRTVAKYRAEEHIPSATQRKRFF"
     gene            1814..2323
                     /gene="raiA"
                     /locus_tag="VLL06_06120"
     CDS             1814..2323
                     /gene="raiA"
                     /locus_tag="VLL06_06120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HSS30576.1"
                     /translation="MKITGRRLVITPALRQHIESRFERLVRYEVTLSHLEVTLGVSKL
                     QHSAEVVCTVQGRRIQAKASTQEMYATIDQLVDRLGAQIRKQKERQTDHKEPTKRSVR
                     KLPPPTLHKEEEELEVIRPVRVVLTLEDAKRRLDPLPGSLLVFTALASGKLQILHRID
                     RHRMVLIDP"
     gene            2333..3199
                     /gene="rapZ"
                     /locus_tag="VLL06_06125"
     CDS             2333..3199
                     /gene="rapZ"
                     /locus_tag="VLL06_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HSS30577.1"
                     /translation="MAGLKLVVLSGLSGSGKSHALKCFEDVGYFCIDNLPPALLPTFV
                     ELCNQQGGEIQSVALGIDVRERVFFSDLVGILERVKVLGHAVELVFFEAREEVLVRRF
                     SESRRPHPLLPHLPVLEGVRFEKERLAELRRHADRIIDTSDLTVHELRELLIRQFRRE
                     DAPHRLTISLVTFGYKFGVPYDIDLLFDVRFLRNPFFVPDLKPLTGEDPRVRTYVLTD
                     PDAVAFIGQLEGLFKFLIPLFERERRSYVNVGLGCTGGRHRSVAIAGRLQESFSALGY
                     QVTVAHRDLNRQ"
     gene            3569..4678
                     /gene="pilM"
                     /locus_tag="VLL06_06130"
     CDS             3569..4678
                     /gene="pilM"
                     /locus_tag="VLL06_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HSS30578.1"
                     /translation="MLSSFKGLAETDLFTMFTPRRQLVGLDIGSSGIKLVQLKESRGR
                     YILQKFGFKPLEPEVIVDGTVMDEGRVVSAIRELFEETKIKVKQVAVSISGHAVIIKK
                     ISLPPMPDEELEGQVRLAAEQYIPFDINEVNIDFSVLPSSEAGGDAQGEMSIILVAAK
                     KDKINELTELVKGAGLLPIVMDVDAFAIENMHAINYPVSQEETTALVNIGASVMNINI
                     VRGGTSLFTRDIPIGGNRYTEAIQREMGMSYEEAEETKKGERSGGNNQAAVTTVVDSV
                     NAEVASEIARTIDYFKSTMQDADVQQVLLCGGGAQVKGLLAQLKDRMHAVVEVANPFS
                     EIDTSGSDFDQNTLAELAPLAAVGVGLALRSVGDR"
     gene            4675..5226
                     /locus_tag="VLL06_06135"
     CDS             4675..5226
                     /locus_tag="VLL06_06135"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HSS30579.1"
                     /translation="MIRINLLPGPKGHKAKPQYDVRAQALLGVGLLFITLAGCWWYSA
                     SLDDEISVRQAEKLDKEKQVAQLKEQVKQVADFESRKKVLEDKNRIIDELEKSRVGPV
                     KVLDHVSQSLEPLKVWLVRVAVTGKEIELEGRAATNDDVVEFVNNLRRTDYFTDIYLQ
                     ESRAAVESQINVYQFKLGFRLKG"
     gene            5232..>5459
                     /locus_tag="VLL06_06140"
     CDS             5232..>5459
                     /locus_tag="VLL06_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30580.1"
                     /translation="MKLPTINLDLLRSIPLVQKVGLLLMVLAGIIVGFYYYVAEPKSA
                     EIEGLQVAIGSLDTEIQTLTIKVKHLDELIAA"
BASE COUNT         1317 a         1251 c         1619 g         1272 t
ORIGIN      
        1 ggggctgatt cttgaggccg ggtctcccga aaccatcgtc aagagtgaaa tggcccgggc
       61 tgtctatcta ggggaacggt tcaagctatg atgcggaagg atctgtacga tgaagcttag
      121 gctcgacctt cgactgagtc aaaagctgat tatgactcca caattgcagc aggcgatcaa
      181 gctcctgcaa ttgtcgagac tcgagcttca gcagagcctc acgcaacatt tgatggaaaa
      241 cccactgctg gaggatctcc ccgcagaagc tgaggatagc gaggccacga gtgcagagga
      301 aaaggcggag gatgcggccg catcagcgga cagtgaacca tcgaatgtgg aggagtccac
      361 cccggaagaa cgcgatacgc cggatgaggc ctcagcggct ggctgggaag agtactttgg
      421 gagtgatcgc cggatcggcg ggtccgagtc tcaatcatca tcacaggatg agttcccgtc
      481 ctatgaacaa accatggcga aagcgacatc cttggaagac catcttctct ggcagctgtc
      541 gttttctggt ttgtccgatc gtgagaaggg gattgggcgg ttgatcattg gtaacctaga
      601 cgatgatgga tacctgcgca tgtctctcgc agagatggtt gccgggaccg acttccagga
      661 gcaggaggcg gagtcagttc tgaaggatat tcagagcttc gatccgaccg gcgtggcggc
      721 acgagacctc gtggaatgcc tgctcttaca aatcggacat ttgaggacga gtaccatggg
      781 ctcattgggg gctcgtcccg gggccttgaa gggatcgatc gttgaagcca ttgttcaaca
      841 ccatctcaaa gacttggaaa agaaacaata tgcgaggatt gcgaaagcct tggatgtcac
      901 agttgatgaa gtgtttcagg cgaccaaggt catcgaagcc cttgagccga agcccggacg
      961 accgtatacg aacacccaga actatgtcat tgtccctgac gtgtttgtcg tcaagaacga
     1021 aggggaatgg gtggtcttac tgaatgacga tgggctaccc cggatgcgga tcagtccata
     1081 ctataagcaa ttgatggggg caggcgaaag tgggtctgcg gagacgaaag cctatctgga
     1141 cgaaaaactt cgggcggcac aatgggtcat tcgcagtatc gagcagcgga ataagacgat
     1201 tgtcaaggtc gtgtcgagta tcgtgaagtt tcaggagcag ttctttgaaa agggcgtgca
     1261 gcatctgaag ccgttggtgc tcaagcaggt ggctgaagat atcgggatgc atgagtcaac
     1321 aatcagtcgt gtcacggcga acaagtatat gtattgtccg caggggatgc tcgaactgaa
     1381 attcttcttc aacgccggcc tgcagcgggc cgaccagcct tccgatatga tgtcatccgt
     1441 aacggtaaga gaaatgattc gaaaaatggt ggcagaggaa gacgcgagtc atcccctcaa
     1501 agatgaagag atcgcggcgc ggttgctcac acaacaagtc gtgatcgcga gacggacggt
     1561 ggccaagtat cgggcggaag aacatattcc atctgccact cagcgtaagc ggttctttta
     1621 gcaggatgtt cacaaatccc tccagcggcg tgcccaccga gtgttcccgc agcctgttag
     1681 actttccctg tccaaccgtg cacgcacgcc tctcttattt ggtctctccc ctggtgatag
     1741 agatgatgag atccaatcgg catagtcgag taccacggag ccaaggttct tcagcttgaa
     1801 aggatgaatg cctatgaaaa ttacaggacg acgcctcgtc atcacaccgg cgctcaggca
     1861 gcatattgaa agtcgatttg agcggctcgt gcggtatgaa gtgacgctga gccatctgga
     1921 agtgaccctg ggtgtgagca agcttcagca tagtgcggag gtcgtctgta cggtgcaagg
     1981 gcgacggatt caggccaagg cttcgacgca agaaatgtat gcgacgatcg accagctcgt
     2041 ggaccgtttg ggtgcgcaga tcagaaaaca aaaggagcgc cagactgatc ataaggaacc
     2101 gacgaagagg tcagtgcgta agcttccgcc tccgacactc cataaggaag aggaagagct
     2161 ggaggtcatt cgcccagtca gggttgttct gactctggaa gatgcgaagc gccggctcga
     2221 tcctctgcct ggttcgctac tggtattcac tgcactggct tctggaaaat tacagattct
     2281 tcatcgtatt gatcgtcatc gaatggtcct gattgatcca taacaggcag gcatggctgg
     2341 actcaaattg gttgtgctca gcggtctttc cggttcaggg aagtcgcatg ccctgaaatg
     2401 tttcgaggac gtcgggtatt tctgtatcga taatctaccg ccagcccttc tgccgacatt
     2461 tgtcgagctg tgtaaccagc aggggggaga aatacaaagt gttgcgctcg gcatcgatgt
     2521 cagggagcgc gtattctttt ccgatctcgt gggcattctt gaacgggtga aggtgctggg
     2581 ccatgcggtt gagttagtgt tctttgaagc tcgtgaggag gtgttggttc gtcgattctc
     2641 ggagtctcga aggccccatc cgcttttgcc gcatctcccg gtattggagg gtgtgcgctt
     2701 tgaaaaggag cgtcttgctg aacttcggcg acatgccgac cgtattatcg atacctcgga
     2761 tttgacggtg catgagttgc gagagctgtt gatcaggcaa ttcaggcggg aggacgcacc
     2821 tcaccggctg accatctcgc tggtgacatt tggttacaag ttcggtgtgc cgtacgacat
     2881 tgatcttctg ttcgacgtcc ggttcttgcg gaatccgttc ttcgttccag acctcaaacc
     2941 cttaaccggg gaagacccgc gcgtgcggac ctacgtgctg acagatccgg acgccgtcgc
     3001 gttcatcgga cagctggaag gcctcttcaa atttctcatt ccactcttcg aacgagaacg
     3061 tcgtagctat gtgaatgttg ggttgggctg caccggtgga cgtcatcggt ctgtggccat
     3121 tgccggtcga cttcaagaaa gcttttcagc cctcggatat caagtgaccg ttgcccatcg
     3181 cgatctcaac aggcaataag cctggacgcc gtcagactct ctgcctcctg ccgccatcgt
     3241 cttcgtctca accacatgtg tgtgtacgaa aagtgatttc cttgacaggt agagcatctg
     3301 gactatactt agcatgtgca tgaagctaat ctgcagtgct ggtgaatcat taagatatct
     3361 gtcttacgta gagtatgcat aggaagcttc tcatcatcag gggaatgcag aatgggcagg
     3421 acgcaggtga atggccggag tcgaatcaat acacattctg aatttggaac gctgaagtgg
     3481 tcgggggtat ggtcatgatg gatcgtctgc gcaatattca cataaagatt tgtcctgcgc
     3541 ggattgtcat gtacagtcgg ggacacatat gttgagctca ttcaaaggtc ttgccgaaac
     3601 ggatttgttc acgatgttca ccccgaggcg acagctcgtg gggctggata ttggatcgag
     3661 tggtatcaag ctcgtccagt tgaaggaaag tcgtggccga tatattctcc agaagtttgg
     3721 ctttaaacca cttgagcccg aagtcattgt cgatggtacg gtaatggatg aggggcgagt
     3781 cgtgtcggcg attcgggagt tgtttgagga aacaaagatc aaggtcaagc aagtcgctgt
     3841 gtcgatctct ggccatgcgg tcatcattaa aaagatcagt ttgccgccga tgcctgatga
     3901 ggaattggag gggcaggtgc ggcttgccgc agagcagtac atccccttcg acattaatga
     3961 ggtgaacatc gactttagtg tgttgccctc ttccgaggcc ggaggcgatg cccagggaga
     4021 gatgtcgatt attctcgttg cggctaagaa ggacaagatc aatgagctga ccgaactcgt
     4081 caagggggcg gggctgcttc caatcgtgat ggatgtcgat gcctttgcga tcgaaaacat
     4141 gcatgcgatt aattaccctg tttcccaaga ggaaacgact gcgctcgtca atattggcgc
     4201 cagtgtgatg aatatcaata ttgttcgagg cggtacctca ttgttcacac gtgatattcc
     4261 catcggaggg aatcgctata cggaagcgat tcagcgagaa atgggaatgt cctatgaaga
     4321 ggcggaggag acgaaaaaag gcgagcgttc gggagggaac aatcaggccg cggtgacgac
     4381 ggtcgtcgac agcgtcaatg ccgaggtggc gtctgaaatt gcgcggacca tcgattattt
     4441 caagtcaaca atgcaggatg cggacgttca gcaggtactg ttatgcggag ggggcgctca
     4501 agtgaagggt ctgctcgcgc aactgaaaga tcgaatgcac gctgtggtcg aggtcgcgaa
     4561 tccgttcagc gaaatcgata cctcagggag cgattttgat caaaatacgt tagccgaatt
     4621 agctccgttg gctgccgtgg gagttggatt ggcccttaga tcggtggggg atcgatgatt
     4681 cgcattaatt tattgccggg gcccaagggg cataaggcaa agcctcagta cgacgtcaga
     4741 gcacaggcgc tgttgggtgt ggggctgttg ttcattaccc tggcggggtg ctggtggtac
     4801 tcggcgtcac tcgatgatga gatcagcgtg cggcaggctg aaaaactcga taaggaaaag
     4861 caagttgcgc aattgaaaga acaagtgaaa caggttgctg actttgaaag ccgaaagaaa
     4921 gtgctggagg acaaaaatcg cattatcgat gaattggaaa agtcacgcgt cggccctgtt
     4981 aaggtgctcg accatgtcag tcagagtctg gaaccgttga aagtgtggct cgtgagagtc
     5041 gcggtgaccg ggaaagagat cgaacttgag ggacgtgcgg cgaccaacga tgatgtggtg
     5101 gaatttgtga ataacctgcg ccgtaccgac tatttcacgg atatctacct tcaagaaagt
     5161 cgagctgcgg tggagagcca aattaatgtc tatcagttta aacttggatt tcgtctcaaa
     5221 gggtaagaat catgaagttg ccgaccatta atcttgacct cttgcggtca attccattgg
     5281 ttcagaaggt ggggctgctt ctgatggttc ttgcggggat tatcgtcggc ttctactatt
     5341 acgtggcgga gccaaaatct gctgagattg aagggctcca ggtagcaatc ggttcgcttg
     5401 acactgagat tcaaacattg accattaaag tcaagcatct tgacgaactc attgcggcc
//
LOCUS       DATIPF010000242         4161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1639970, whole genome shotgun sequence.
ACCESSION   DATIPF010000242 DATIPF010000000
VERSION     DATIPF010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639970"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VLL06_06145"
     CDS             complement(<1..361)
                     /locus_tag="VLL06_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSS30581.1"
                     /translation="MPIEKFTAQVDMIKDLTHDVRQLDLRLIEPKTIVFKPGQFISFE
                     MPHPRTGHLVTRAYSIASQPSRSDVITLLFNLVPGGPGSGLLFHLNAGEKTHFKGPAG
                     NFYLREDLGRDLLFIASG"
     gene            complement(432..1445)
                     /locus_tag="VLL06_06150"
     CDS             complement(432..1445)
                     /locus_tag="VLL06_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HSS30582.1"
                     /translation="MGNGRVTSAKIMQLGTGFWGSKTLLSAIELGLFTELAKGPLDGK
                     TLGERLGLHPRSARDFFDALVALGMLKRNGVRYSNTMETGRFLDRAKPSYVGGILEML
                     NARLYRFWGSLTEALHTGKSQNEAKTGEDFFGTLYADPQRLENFLKAMTGLSIGSAQL
                     IAKKFPWKKYHTFTDVGCAQGGVAVEIALAHTHLTGGGMDLPMVQPIFEAYAKTRGIK
                     KRLTFHRGDFFKDPLPKSDVIIMGHILHDWNLEEKMMLLRKAYDALPPGGAVIVHEAL
                     IDDARKHNAFGLLMSLNMLIETHGGFDFTGADCRKWMKDVGFTRTRVERLAGPDGMVV
                     GFK"
     gene            complement(1627..1926)
                     /locus_tag="VLL06_06155"
     CDS             complement(1627..1926)
                     /locus_tag="VLL06_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30583.1"
                     /translation="MAEATDVYADQFQLNLGPLGCTLNFQVSGANPVAPGTMPPIERV
                     ATIRLSLQHLKAMSFIFHKQIVAYETQTQLNIGLPVDVLRAMQIRQEDWEAFWRP"
     gene            complement(2094..2552)
                     /locus_tag="VLL06_06160"
     CDS             complement(2094..2552)
                     /locus_tag="VLL06_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30584.1"
                     /translation="MEEIRSYIKTLLRKMGLQIAEHSQDDIVLQRSKHHQSERRRETR
                     RPMISPCTYGLMRSTDHNGAIREEGYGTAVNDSPTGMRLLLGVALTKGQFLEIQTDDR
                     TLDHPVCLVEVCWTKPLRENPQEHTQENAQEALYLVGCRQSSGSTHDEAI"
     gene            2815..3396
                     /locus_tag="VLL06_06165"
     CDS             2815..3396
                     /locus_tag="VLL06_06165"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSS30585.1"
                     /translation="MPRPKTFNPDDALDKAMQVFWHKGYEATSMEDLLKAMDLNRGSL
                     YDTFGDKRQLFLNVMDRYCRTFVGPKLLLLDQPGPALPTLHRFINGMIEGGLADPQRR
                     GCFISNTVMELSPHEKEIAGTLRQALKMAEDAFFKVLVRAKEQGELKDEKDPRALARF
                     LTTMMQGIVVMIKAGTSAEVVTQTAETGLSILD"
     gene            3480..3692
                     /locus_tag="VLL06_06170"
     CDS             3480..3692
                     /locus_tag="VLL06_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30586.1"
                     /translation="MTCQRCDGLMVRERYDDLELGSAGHELPSWRCLNCGAIVDPVIL
                     AHHHTFDQAERDRLPRRRVKKPVMVG"
     gene            3771..>4161
                     /locus_tag="VLL06_06175"
     CDS             3771..>4161
                     /locus_tag="VLL06_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSS30587.1"
                     /translation="MRLEKKVAIVTGGGTGIGEAIAKVFAREGATVAITGRRKEELER
                     VVKAIEQNGGHALALQGSVTNDSDVREAVEATVRTCGRLDILVNNAGNLFYAGPLHET
                     PDQIWDETFDLFMKGPFRFIRAAIPDML"
BASE COUNT          941 a         1149 c         1146 g          925 t
ORIGIN      
        1 tgccgctggc gatgaacagc aaatcgcgtc ccagatcctc tcgcaaataa aaatttccag
       61 ccggcccttt gaagtgtgtc ttctcccctg cattgagatg gaacagcaac ccggagccag
      121 gtccgcccgg caccaggttg aatagcaggg taatcacgtc cgagcggctg ggttgtgagg
      181 caatggaata ggcccgggtc acgaggtggc cggtccgtgg gtgcggcatc tcgaacgaaa
      241 tgaactggcc tggcttaaaa acaatggtct tcggttcgat cagccgaaga tcgagctgac
      301 gcacgtcatg cgtcagatct ttgatcatgt caacctgtgc ggtaaatttc tcgatcggca
      361 tggaggaaga ttctagcagg atgctgaaaa gatctgccag cctggctgga cgctatctgg
      421 ccgagtgctc tttacttgaa tcccaccacc ataccgtccg gtccggccag tcgctcgacg
      481 cgcgtgcgcg tgaacccgac atctttcatc cacttccggc agtccgcgcc ggtgaagtcg
      541 aatccgccgt gggtttcaat gagcatattg aggctcatga gcaacccaaa tgcattatgc
      601 ttgcgtgcat cgtcgatcag ggcttcatgg acaatgaccg ctccgccagg gggcagcgcg
      661 tcgtaggcct tgcgaaggag catcatcttt tcctccaggt tccagtcgtg gagaatatgg
      721 cccatgataa tcacgtcgga cttcggcagc gggtctttga agaaatctcc cctgtgaaag
      781 gtcagccgct ttttaatacc tcgcgtcttc gcgtaggcct caaagatcgg ttggaccata
      841 ggcaggtcca taccgcctcc ggtcagatgc gtatgcgcaa gggcgatttc gactgccacc
      901 ccgccttgcg cgcagcccac gtccgtgaac gtatggtact tcttccaagg aaacttctta
      961 gcaatgagtt gcgcggaccc gatactcagg ccggtcatgg ctttgaggaa gttttccaac
     1021 cgttgtggat cggcatacaa ggtcccgaag aagtcctcac cggtcttggc ttcattctgc
     1081 gatttgcctg tgtgcaacgc ttcggtcagc gagccccaga atcgatagag ccgcgcattc
     1141 aacatttcca aaatgccgcc gacatacgaa ggcttggccc gatcgagaaa ccgtcccgtc
     1201 tccatggtat tggaatagcg cactccgttg cgtttcaaca tgcccagcgc gaccaacgcg
     1261 tcgaagaaat cccgcgcgct gcgagggtgg aggccaagcc gttcgccaag cgtcttgcca
     1321 tcgagcggac ctttggcgag ctcagtaaag agtccgagct caatggcgct gaggagcgtc
     1381 ttcgaccccc agaaccccgt accgagttgc atgatcttcg ccgaagtcac tcgaccgttg
     1441 cccattgaat cactccttgc ctagtcactc actccacacc cgatgatttt cagatattcg
     1501 gcctctgctc gtgcctcgca gaagccattg gtgaactaaa acatttgagg aatgtttggg
     1561 actgaaaacg acacagatat ctgtgaatcg tatgccgcaa tagaatggca tgactcgcac
     1621 agactcctaa ggccgccaga acgcctccca atcctcctgc cggatttgca tggctcgcag
     1681 cacatcgaca ggcagaccta tgttcagctg agtttgggtt tcataggcca cgatctgctt
     1741 gtgaaatata aaagacatcg ctttgagatg ctgaaggctc aaccgaatcg tggccacacg
     1801 ttcaatcggc ggcatcgtcc ccggagccac cggatttgct ccggagactt gaaaattcag
     1861 cgtgcatccg agcgggccaa gattgagttg aaattggtcg gcatagacat cagtggcttc
     1921 ggccatactt ccccctatga gtgtggttaa tgacgccggg gaatattatc ccccattgta
     1981 catttggtca attgactgaa cgcttaccag tggtcaaacg gctgggcgaa cacatgggcg
     2041 ggtcggaaag aaatctgatc acaggtgggt cctacgcaga atgccggtcg cgcttagata
     2101 gcctcatcgt gcgtgctgcc tgagctttgg cgacagccta cgaggtacaa tgcctcttgc
     2161 gcattttcct gcgtgtgctc ctgcggattt tctcgcagag gtttcgtcca acagacttct
     2221 acgagacaga ccggatggtc gagcgtccga tcgtcggttt gaatttccaa aaactgacct
     2281 ttggtcagcg cgacgccgag caggagccgc attccggttg gactatcatt cactgccgta
     2341 ccatacccct cttcgcgaat cgcgccattg tgatcggtcg agcgcatcaa gccataggtg
     2401 caggggctga tcatcggcct tcgtgtctcc cgccgacgtt cagattgatg atgcttactg
     2461 cgctgcagca caatgtcatc ttgagagtgc tccgcaatct ggagtcccat tttccgcagc
     2521 aacgttttga tgtatgaccg tatctcttcc acgatctgtt tctctgaatg ttccccgccg
     2581 tcacgcgaac ttttgtcgtt cccccgacag ttgacgcgtt attatactgc gtcacggtca
     2641 tggtatagaa gcgaggagcg atctctagaa cgataaaaat tacgttcgct atttcccatc
     2701 catactcata tgcaggcacc atccggtagg agggatgacg gggggtctat tattgaccga
     2761 tcgttccaat atatgctacc ttggcgcccg agtcaggaat accggagagc tcccatgcca
     2821 cgcccgaaaa cgttcaatcc ggacgacgcg ctcgacaaag cgatgcaggt cttctggcac
     2881 aagggctacg aagccacatc gatggaagat ttgctgaagg cgatggacct caatcgcggc
     2941 tccctctatg acacattcgg cgataagcgc caactgttcc tgaacgtgat ggaccgatac
     3001 tgtaggacct tcgtcgggcc gaagctcttg ctcctcgacc aacctggccc ggcactccca
     3061 accttgcatc gattcatcaa tggcatgatc gaaggcggtc tggccgatcc gcagcgccgc
     3121 ggctgcttta tctctaatac ggtgatggaa ctgtccccgc acgagaaaga aattgccggg
     3181 acactccgcc aggcactcaa gatggcggaa gatgcattct tcaaagtcct ggttcgtgcg
     3241 aaagaacagg gtgaactgaa ggacgagaaa gatccgcgtg cactggcgcg ctttctcacg
     3301 acgatgatgc aagggatcgt ggtgatgatc aaagccggaa cgtcggctga ggtggtaacg
     3361 cagacagcgg agaccgggct ctcgatcctg gattgagccg atctcttttt ttgacatatt
     3421 atggaacgat cgttccgcat gtgaagtggc agaggccacc ccggggaagg aggcgccata
     3481 tgacctgtca acgttgtgat gggctgatgg ttcgagaacg gtatgatgat cttgaattgg
     3541 gctctgcggg gcatgaactc cccagctggc gttgtctcaa ttgcggcgcg atcgtcgatc
     3601 ctgtcatcct ggcccatcat cacaccttcg accaggcaga gcgagatcgg ttgccgcgac
     3661 ggcgtgtgaa gaaacctgtg atggttggat aggggacatg tcaccattca tcgggtccag
     3721 agatatccat caagacaagc tcaactcaat tattccaagg agggcgcacc atgagactcg
     3781 aaaagaaagt ggccattgtg acgggcggag gaaccggtat tggagaagcg atagccaaag
     3841 tctttgcgcg ggaaggcgcc acggtcgcga tcacggggcg ccggaaggaa gaattggagc
     3901 gggtcgtcaa agccatcgag cagaacggcg gccatgcact cgcgcttcag ggcagcgtca
     3961 cgaatgattc agacgtccgg gaagcggtgg aagccacggt cagaacatgt ggccgtcttg
     4021 acatcctcgt aaacaatgcc ggtaacctgt tctatgccgg accactccac gaaacacccg
     4081 accagatatg ggacgagacc tttgatttgt tcatgaaggg tccgttccgg ttcattcgag
     4141 cggcaatccc cgatatgctg a
//
LOCUS       DATIPF010000243         3133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_666556, whole genome shotgun sequence.
ACCESSION   DATIPF010000243 DATIPF010000000
VERSION     DATIPF010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666556"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(699..1523)
                     /locus_tag="VLL06_06180"
     CDS             complement(699..1523)
                     /locus_tag="VLL06_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HSS30588.1"
                     /translation="MIRLLLTSIALFVLLVTAACGITVKPGERGLFWRPLSEGLSNEP
                     LKDGFYWQAPWNNLYRYDIRWQSHTEDIDALSKDDLQVSIKAAIILRPISKDLYFLQQ
                     ELGPDYYVRIVKPQFMAAVRSVVSGYNMVTVPEMSTEIASKVRAVVVENLKGRHLEIH
                     NVALSDVDFPPIVLRSIEQKQAKEQEKEQKEFELTIASKDAEIARTRAKGEGDAIRIR
                     AEGEAEGLRIRAIGQAQAQETITKTLTPAYLQYKLYDSSNSKMIILPENMGGPVIK"
     gene            complement(1620..2105)
                     /locus_tag="VLL06_06185"
     CDS             complement(1620..2105)
                     /locus_tag="VLL06_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30589.1"
                     /translation="MRSCLLRLYQVLILAEQVTYERIHGRVASPSELLRLVVNDSWFT
                     WLHPFSLLVQRIDQLLDRDSELSLVEVKQWLSHIRTFIRLSEEGDGFARSYYEALVRE
                     PDVVYAHIEVIAQAMLMSDYHPAAQDQTESDPHPLLDCNPQEQHDVSTRVFPRGNPSR
                     N"
     gene            complement(2472..3080)
                     /locus_tag="VLL06_06190"
     CDS             complement(2472..3080)
                     /locus_tag="VLL06_06190"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HSS30590.1"
                     /translation="MAIINHATSTWQNFMQDPDMSGFRSHVKGGQGVLIFPRLMKGAF
                     VLGAEGGNGVLLVRNSKTGSWSEPVFYEISSASFGLQAGMETSETILVIQTVKGVESL
                     LKNTIKLGTDVSAAIGPKGGGIEGVTSMSIGMDFVTYARAKGIYAGVSIEGASISTRD
                     DLNKAYYGSSVSPSDIVLVRSVKSHPQSQVLHQAVVKGTIGG"
BASE COUNT          775 a          828 c          790 g          740 t
ORIGIN      
        1 gccaggttta ggccatgcac agagtaggag gatttgtata tggcatcgac gtgatgagtc
       61 ttctcgtgtg tgtgtttgtt gggattcggt ctctccaacg cgcgctcgca atcgaatctt
      121 tggagacgga tgtaacagaa caaaatacca tgagaaacgg cagttatcct ggttcgttca
      181 aacaagagca cagacacacg aatgggaacg tttggaagat aatgagtatg tggtcaagcg
      241 gagtgatggg aagcaaggag cctgcatgtc gactgaagca cccaaatgcc aagagttgag
      301 gcgaaagtgt tccccaatcg gtagcgggaa gggtactcat ataccctaaa tggtgcctct
      361 tctgcagtcg ctcagtacga actcttcgaa aaagcacaat ctagaaacta tgatggaagg
      421 cccgatgctc ggcaccgggc ctttgttttg gctgactcat gccgaagggt tattggagaa
      481 gaatccgtgg ctgacatcgt ggcgaaacaa taaagcgagc cgtatcagga ccgactgtct
      541 agtcactcat ggatcaagag caaaatcctg agtacaccga gaaggaaggg cggcatgaat
      601 ggttcggtcg cttccgcaag aaaggaaagg aatgaacggt gggtggagaa agcattagca
      661 cggtgaaaca aggaaacccc gatgaacagc ttgagctgtt acttgataac ggggcctccc
      721 atgttctctg gcagaatgat catcttggaa ttcgaactat catataactt gtactgcaga
      781 taggcgggag tgagggtttt cgtaatggtc tcctgcgcct gcgcctgacc aatggccctg
      841 atacggagcc cctcagcctc cccctcggcg cgaattcgga tcgcatcccc ctcgccctta
      901 gcccgcgtgc gggcaatctc ggcatccttg gatgcgatgg tcaattcgaa ttctttttgc
      961 tctttttcct gttccttcgc ctgtttctgc tcaatcgaac gtaggacaat gggcggaaaa
     1021 tcaacatcgg acaaggcgac attgtgaatc tccaagtggc gacctttgag attctccacc
     1081 acgacggccc ggactttgct cgcaatctca gtgctcatct ccggcaccgt caccatgtta
     1141 tagccagaga cgacactgcg taccgccgcc ataaactggg gtttgacaat ccggacgtaa
     1201 tagtccgggc ccagctcttg ttgcaaaaaa tagaggtctt tcgaaatggg ccggaggatg
     1261 atcgccgcct tgatggacac ctggagatca tccttactca aggcatcgat gtcttccgtg
     1321 tggctctgcc accgaatatc gtagcggtac agattattcc agggtgcctg ccagtaaaat
     1381 ccatccttca gcggttcgtt agaaagccct tcggaaagcg gacgccagaa caaccctcgc
     1441 tcgcctggtt tgacggtgat gccgcaggca gcggtcacga gcaacacgaa gagcgctata
     1501 cttgtcagaa gtagtcgtat catagctccc cttagtgccg catgcatgac gtgtgaccct
     1561 gtcaaccgac cgatccctaa tgactgtcca gatgcggatc agcaagccga tcacttgttt
     1621 caattccggg atggattccc acgaggaaaa actctagtgc tcacatcatg ctgttcctga
     1681 gggttacaat ccagtaatgg gtggggatcc gactctgttt gatcttgggc agcggggtga
     1741 tagtcactca tcagcatcgc ttgggctatg acttctatgt gcgcgtagac gacatcgggc
     1801 tctcgtacca gggcttcata gtagctccgt gcaaacccgt ctccttcctc cgaaagtcgg
     1861 ataaaggttc gaatgtggct caaccattgc tttacctcaa caagcgacag ttcggaatca
     1921 cgatccaaga gttgatcgat ccgctgcacc agtaaagaaa agggatgaag ccaggtaaac
     1981 cacgaatcgt tcaccactaa tcgcagtagt tcgctgggtg aagcaactcg cccgtgaatg
     2041 cgctcatagg tcacttgttc tgcgaggatt aggacctgat agagacgcaa gagacagctt
     2101 cgcatgtcga gcagtttctt acgaaggcag gatgtcgcga tcgaactagt tggcaattcc
     2161 ataacgtaga cgagaggtta ccacgtcatt tgatccaggc tagctaggag actcccctgc
     2221 cataattcat gggcacaaga gaaccattat cgcggatgga tcgaatgcgg agaagaacag
     2281 ttactgccca aaggagatgt tcacatcgaa aaagccgcta ttgaaacgtt acgccaagga
     2341 tcatgccgat tttgagattc gtctcacccc gggacacaac cgaaaatctc cgtttcacgc
     2401 tcgctcacgc ctagctgatt caatgatgtg agcagaggat tggtccacag ttcctcactg
     2461 tcaccctacg gttacccgcc tatagtccct ttgacgaccg cttgatgcaa cacctgtgat
     2521 tgaggatgtg acttgacgct tcgaacgagc acgatgtcgc tcggcgacac cgaggagcca
     2581 tagtacgcct tgttcaaatc atctctcgta cttatggacg ccccttctat ggagacaccg
     2641 gcatatatcc cctttgcacg ggcgtaggtg acaaaatcca ttcctatact catcgaggtc
     2701 actccctcta tgccgccacc cttcggaccg atcgcagccg atacatcagt cccgagcttg
     2761 attgtattct tcaagagact ctcgactccc tttactgttt gaatcacgag gatcgtctca
     2821 gacgtttcca tgcccgcctg taatccgaag ctggccgatg atatttcata aaagaccggc
     2881 tcactccagc tgcccgtctt actattccga acaagtaaca ccccatttcc cccttccgca
     2941 ccgaggacaa atgctccctt catcaatctc gggaagatca gcacgccctg ccctcccttt
     3001 acgtgagagc ggaaaccaga catatcagga tcctgcatga agttttgcca ggtcgaggtg
     3061 gcatgattga tgatcgccat agcttcccgt tcttgagctt attcttgaag attttccgct
     3121 gctgtatgtt gcg
//
LOCUS       DATIPF010000244         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1019649, whole genome shotgun sequence.
ACCESSION   DATIPF010000244 DATIPF010000000
VERSION     DATIPF010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019649"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VLL06_06195"
     CDS             <1..230
                     /locus_tag="VLL06_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30591.1"
                     /translation="ASSVATRDLIGLRPYRSVGQHHEVADTIFFGAFLRPESNRAGTA
                     GDSGAKSLRLRAPVSLGDCAISQRTVMKNAG"
     gene            232..1032
                     /locus_tag="VLL06_06200"
     CDS             232..1032
                     /locus_tag="VLL06_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HSS30592.1"
                     /translation="MPHPSGLITLLTDFGDRDAFVASMKGVVLTINPQATLVDISHQV
                     PPHSIEEAAYLLKSCYRYFPDGTVHVAVVDPGVGSARRPLIAKSERYYFLAPDNGLLT
                     PILAEDRSMEVREIENANYRLTPPGHTFDGRDIFAPAAAWLTKGEPVSSFGRVIDDPV
                     TRSIAVPVWQGPSLVGTIEYVDRFGNLISNVTVKHLQEFLATVERQHPIMHIGTYTIH
                     GLVTSYSEGKSDIPSALINSCGKLEIFLDRHHAAQRLQLGVGGEIRLC"
     gene            complement(1044..>2170)
                     /locus_tag="VLL06_06205"
     CDS             complement(1044..>2170)
                     /locus_tag="VLL06_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSS30593.1"
                     /translation="QSKARIILIEKESTCGAHASGRNSGVLHAGFYYSPDSLKAKFTK
                     LGNEQLTAYCEEKHISLNKCGKLVVAKDEGDLKSLDELFRRGQANGIELQELTDIEAK
                     KIEPRVRTYQRALFSPRTSTVNPLHVVNAMQEDAKREGVQIRVDTAYVGKDEQRVRTS
                     HDSIEAGYVVNAAGLYADKIALEYGFSQKYRILPFKGLYLYSNEPPGSIRTNIYPVPD
                     LRNPFLGVHFTITADGKTKIGPTAIPAFWRENYEGLNNFKLSELIEVAGRGLGLLTGA
                     QFDYRRLAVEEIAKYSRSKMVSLASVLADGVVEKNYQAWGRPGIRAQLLDITKKKLEM
                     DFVLEGDHRSMHVLNAVSPAFTCSLPFASYVCDHIEKAIG"
BASE COUNT          441 a          578 c          616 g          535 t
ORIGIN      
        1 ctgcaagctc ggtggcgacg cgcgatttga ttggcctgcg accgtaccgt tcggtcggtc
       61 aacatcatga ggtggctgac accatttttt ttggggcctt tctccgtcct gagagcaata
      121 gggcaggaac cgccggagac agcggagcca agtccttgag actccgcgcg ccagtctcgt
      181 tgggcgactg cgcgatttcg caacgaaccg tcatgaagaa tgcgggctaa catgccgcac
      241 ccttccgggc tcatcacact gttgaccgat ttcggcgatc gggacgcgtt cgtggcgagt
      301 atgaagggag tggtcctcac gatcaatcca caggccaccc ttgtcgatat ctcgcatcag
      361 gtgccgcccc attcgattga agaagccgcc tatcttctga agtcctgcta tcgatatttt
      421 ccggacggca cggtgcacgt ggccgtcgtt gatcctggtg tcggcagcgc gcggcgccct
      481 ctcatcgcga agagcgagcg gtattacttt ctagcccccg acaatggtct gttgacgcca
      541 atccttgccg aggaccgcag tatggaagtg cgtgagattg agaatgcgaa ctatcgattg
      601 acaccaccgg gccatacctt cgatggccgc gatatcttcg ccccagctgc ggcgtggttg
      661 acaaaggggg agccggtttc gtcatttggg cgagtgattg atgatcccgt gacgcgttcg
      721 attgccgttc cggtctggca gggcccatcg ttggtcggaa cgatcgaata cgtcgatcga
      781 tttggcaatc ttatttcgaa tgtgacggtg aagcatctac aagaatttct ggcaacggtg
      841 gaacgacaac atccgatcat gcatatcggg acctatacca ttcacgggct agtgaccagc
      901 tacagtgaag ggaaaagtga tattccctcg gcgctgatca atagctgtgg aaagctggaa
      961 atctttctgg acagacatca cgccgcccag cgcttacaac ttggtgtggg tggggagatt
     1021 cggctgtgct aatggaggga gtactaccca atcgctttct cgatgtgatc gcagacgtag
     1081 cttgcaaatg gaagggaaca agtgaaggct ggtgagacag cattgagcac gtgcatagag
     1141 cgatggtcgc cttctaacac gaaatccatt tcaagttttt tctttgtgat gtcgagcagt
     1201 tgggccctga ttcctgggcg cccccatgct tggtaattct tttctaccac accgtcggcg
     1261 agcactgagg ccagtgagac catcttgctc cgggaatatt tggcaatctc ttctaccgcg
     1321 aggcgccggt agtcgaactg cgcacccgtg agcagtccta acccacgacc agcgacttcg
     1381 atcagttcac tcagtttgaa attgttgagt ccctcgtagt tctctcgcca gaaggccggg
     1441 atggccgttg gtccgatttt ggttttgcca tctgcggtga tcgtaaagtg gaccccaaga
     1501 aacgggtttc tgagatccgg gactggatag atattggtcc gaatggaacc gggaggctcg
     1561 tttgaataga gatacaggcc tttgaatggg aggattcgat atttctggga aaatccgtac
     1621 tccagagcaa ttttgtcggc gtagagcccc gccgcattga ccacatatcc agcttcgata
     1681 ctgtcgtggc tggtgcgaac gcgctgctcg tctttcccta cgtaggccgt atcgacgcgt
     1741 atctggaccc cttcccgctt ggcatcttcc tgcatcgcat tcaccacgtg cagtggattg
     1801 accgtggagg tgcgaggtga aaaaagtgct cgttggtagg ttctcaccct gggttcgatc
     1861 ttcttcgctt cgatgtccgt gagctcttgc aactcaatcc cattggcctg accccggcgg
     1921 aacaattcgt cgagtgattt caagtcccct tcatccttgg ctaccaccag tttgccgcat
     1981 ttattcagcg agatgtgttt ttcctcgcaa taggccgtca gctgctcgtt gccaagcttc
     2041 gtaaacttcg ccttgaggct gtcgggggaa tagtaaaacc cggcgtggag gacaccgctg
     2101 ttgcgcccgc tggcatgggc gccgcaggta gattcttttt cgatcaagat gatgcgggcc
     2161 ttgctctggc
//
LOCUS       DATIPF010000245         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_220014, whole genome shotgun sequence.
ACCESSION   DATIPF010000245 DATIPF010000000
VERSION     DATIPF010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220014"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VLL06_06210"
     CDS             <1..242
                     /locus_tag="VLL06_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30594.1"
                     /translation="FVEPHLKPQLTPGQRPENVRFGLYSNRSEKASQIVAVPLDMLMV
                     DRMPTRLDPKEPGSAVLLNVDLQTSATRTSSQPRP"
     gene            570..932
                     /locus_tag="VLL06_06215"
     CDS             570..932
                     /locus_tag="VLL06_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30595.1"
                     /translation="MKAILALSTGFIVLFTISLALANPILLPKHPGYPMGKAVDPVSG
                     QSLANDPGQPNATGENSLAQASKFDDVHVKQNLSINQNDQRLLEKPGAGLLPKVQGPN
                     IKIEPPVKEATKMSESPK"
     gene            1230..>2076
                     /locus_tag="VLL06_06220"
     CDS             1230..>2076
                     /locus_tag="VLL06_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30596.1"
                     /translation="MIMSEPVSKCLTRHIFSITVSALLGAGVAIGAVAPASAYEVWLT
                     DQSDTAKESGGFLYIYDGTALAANPAAAKPAVTIDLSGEINTFCEAATKKPVRRPHML
                     FFNKDQDHAILSFLSGHVLFMDAKTRKPEACLSMGKNVHAAWPTADQKMAIAANIAEK
                     KFIRIWTDYAKHSFSFDPEKDVVNLAALEGGERPDASPICPITESSSRYAFVTLRGGG
                     LLVFDVTTTPMRVITTLNNNEIHPAGCGGIQVGETMYINSGGGWPIAPLSYDVYALEL
                     STLPKS"
BASE COUNT          453 a          577 c          571 g          475 t
ORIGIN      
        1 gatttgtgga gccacacctc aaacctcagc tgacaccagg tcagaggcca gagaatgtcc
       61 ggttcgggct ctattccaat cgctcggaaa aggcttcgca gatcgtcgcg gtgcccttgg
      121 atatgttgat ggtcgatcgg atgccgaccc gactcgatcc caaggagccc ggcagcgcag
      181 ttctgctcaa tgtggacttg cagaccagcg ccacccgtac gtcgtctcaa cccaggccgt
      241 aggggtcagt tcgagtcatg ccttctaccc cggtacacga tctgccgaca tccgcgttgt
      301 gctcagtccg aaaaaatcat cgacactcct tgggcatcgg cctaccgtga tttcactgat
      361 ccaggccgat gccctgagcc gattctggcc cacttgactc tgtttaaacg gggagcgatg
      421 ctagaacacg ttccagaggt gtagtatgac accgggtcac ggtttccacc gctgttgtgt
      481 cctccctctc cgtgcttttc ttgtgttccc gccgctatca catcggcagg atgccgtggt
      541 cttccccctt ctcaccaagg aggtgcgtca tgaaggcgat tcttgcgcta tccaccggtt
      601 tcatcgtgtt gttcacaatc tctctcgccc tggccaaccc gattctgttg ccgaagcacc
      661 cggggtatcc catgggcaag gccgttgatc ccgtcagcgg ccaatcgctt gcaaatgatc
      721 ctggtcagcc caatgccaca ggagagaatt cgctggccca ggcatcaaag tttgacgatg
      781 tccatgtaaa gcagaatctg tcgatcaatc aaaatgacca gcggttgttg gagaagcctg
      841 gtgcggggct gctgccgaag gttcaaggac ccaacatcaa gatcgagccg ccagtgaagg
      901 aagccacgaa gatgagtgaa tcgccgaagt gaaggagcct gatgatacga tgaagaactt
      961 ggccgaccct ggctgatata cagtccaggg ttcggccttg aaatagggaa tggtgttatc
     1021 caatcatcgc gtcctcttcg cacgcaacga taatctgttt ggaggaataa acctaatgta
     1081 tttagcagtg cgacatcctg ctaggtgaat agtcattgat ctcttcttgg aatccttcta
     1141 ccctatgcct ccacgtaccc gcgttatgcc atggcttatc aacacacagc agtatgtgca
     1201 tcgctgaaat ccgattgaag gaggcccaga tgataatgtc cgaaccggtg agcaagtgtc
     1261 tgactcgtca tattttttcc atcacggtga gcgccctgct tggggcggga gtggcaattg
     1321 gtgccgtggc tccagcttcc gcctacgaag tgtggctgac ggatcaatcc gatacggcga
     1381 aagaaagcgg cggctttctc tatatctatg acggaacggc gcttgcggcg aatcctgctg
     1441 cggcaaaacc ggccgtcacc atcgatctgt ctggcgagat caacacattt tgcgaagcgg
     1501 cgaccaagaa gccggtccgg cgcccgcata tgctgttctt caacaaggac caggaccatg
     1561 cgatcctctc gtttctgagc ggccacgtgc tatttatgga tgcaaagacg cgaaaaccgg
     1621 aggcttgctt gtcgatgggg aagaacgtcc atgcggcttg gccgacggct gaccagaaga
     1681 tggcgattgc ggcgaatatc gccgagaaga agttcatccg tatctggacc gattatgcca
     1741 agcattcatt tagcttcgat cccgagaagg acgtggtgaa tctcgctgcg ctggaaggtg
     1801 gagagcgtcc ggatgcgtcg ccaatttgcc cgatcacgga gtcatccagc cggtatgcct
     1861 ttgtcacgct gcgtggagga ggattgctgg tattcgatgt caccaccact ccgatgaggg
     1921 tcatcaccac gcttaacaat aatgagatcc accccgctgg ctgtggcggt attcaggtcg
     1981 gcgagacgat gtatatcaat tccggtgggg gctggccgat cgcacctctc tcttacgatg
     2041 tgtacgcgct cgagctgtcg acccttccga aatcgg
//
LOCUS       DATIPF010000246         4218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_753351, whole genome shotgun sequence.
ACCESSION   DATIPF010000246 DATIPF010000000
VERSION     DATIPF010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753351"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2177)
                     /locus_tag="VLL06_06225"
     CDS             complement(<1..2177)
                     /locus_tag="VLL06_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HSS30597.1"
                     /translation="MHDYLHRQVLRNLSFAAFIGVCAFTLFSDQSSADSPPPSISSDS
                     DACETAEECFQAAALPKERLGTALNKEQVLSLKLSRLQRLMERFPATLWAKRAGLLSG
                     VLLLERNPAVAIQFLRTSQRDFPILDDYIRLWTGEALLNLGDAKQAADMFEGIPQAVP
                     DSYLLAKAAYRAGEAWYQASSCLEATAWFARAVELDDKEPGTPQAFLRRAACQLRDNN
                     IPKGRETLNQLWVRFAYSQEAKEAEALLVSNLGGEPWVVQSSDRLARAQVFLGQSFHT
                     EAIEEFKKFLAADPKSPRRAEVKLKIGIAQVRLKLYDQARETFYALVKDGGVGSLEAS
                     VWLARVYLRQGQGDKLLDLARTLPTSLLSAEQKGQITLFAGIWLEDQSRFDDAIVRYR
                     QVAKVGEPASQRAEARWRVGWVYYRTARYREAGDELRLLADQHDGDFEPQALYWMARA
                     AELTQQPYARDVYVQLCQRYVYTYYCQLARERIDLPLSESIVTEPISATAPANGGTPM
                     SGTATGSVSSRVEVEQQSAYRRAIELKTLGLDSDAARELEGLTDRYSRDPDVLMAVAT
                     MLNEVGAYHHALRLARAKFRDKLERTGGIVDPSLWKVAYPTGLLPTIKGQGANGVDPY
                     LVAAIIREESQYDWRAVSRVGAIGLMQVMPGTANTVAQRLGLSAVGRDDLFDQETNIR
                     IGVHYVEQLLEQFSGNLMHTIASYNAGPIVVGTWIALHRGRSQ"
     gene            2336..3400
                     /gene="rlmN"
                     /locus_tag="VLL06_06230"
     CDS             2336..3400
                     /gene="rlmN"
                     /locus_tag="VLL06_06230"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HSS30598.1"
                     /translation="MNLLSLDEVGMCRLVQQFGWPRYRTGQILRWLYQRRAKDINQMT
                     DLGQAERTALAALATIPRAVGCTVFRSIDHTRKLILTLNDGLSVETVMIPDEDRLTLC
                     VSTQVGCTLDCGFCLTGTMGLKRNLKAHEIMEQVLTAQDRLDSGERITNLVFMGMGEP
                     LANMESLSEAIRRLTNKPWGLGWSPRRITVSTAGLAARLKDVAPLGVNLAISLNATTD
                     DQRASLMPAVNGIASLKTLLAACRRYPLPPTRRLTFEYVLLGGVNDHADDARRLIKLV
                     RGIACKINLIPFNEFPGSSFCRPSEDNIAAFQAIVRQAGLDVFVRKSRGRDVLGACGQ
                     LGNLAPEYATHALTQIESRC"
     gene            3456..>4218
                     /locus_tag="VLL06_06235"
     CDS             3456..>4218
                     /locus_tag="VLL06_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSS30599.1"
                     /translation="MHIGCLIAASSLAFVFIASSAVAAEPREFILSNGMKVLLVEVPK
                     APVATVQVWYKVGSRNEVMGRAGLSHMLEHMMFKGTAKYPKGTFSRLIRKNGGTDNAF
                     TSQDFTAYFENLAADRVELALELEADRMQGLMLDMSELTTEREVVKEERRLRTEDDPQ
                     GALVEALFAQAYLSHPYHWPVIGWFSDLDAMTLDDLQRHYDTYYSPNNATLVVVGDIK
                     ADSLLAMIKHLFEPIPRGPEPKPIATMESEQKGERR"
BASE COUNT          943 a         1286 c         1104 g          885 t
ORIGIN      
        1 tcctgacttc ggccgcgatg cagtgctatc caggttccga ccactattgg ccctgcattg
       61 tatgaggcga ttgtatgcat cagatttccg gaaaattgtt cgagtagctg ttcgacataa
      121 tgaacaccaa tccgaatatt ggtctcttga tcgaagaggt catcccttcc gaccgcggac
      181 agcccgagcc gctgcgcaac agtattcgcg gttcccggca taacctgcat caggccgatc
      241 gcaccgaccc gcgacaccgc gcgccaatca tactgactct cttctcgaat gatcgccgcc
      301 accagatagg ggtcgacacc gttcgcaccc tgccccttga tcgtaggaag gagaccagtc
      361 ggataggcga ccttccacag cgacggatca acgatgccac cggtccgctc caatttatcg
      421 cggaacttgg ccctcgcaag tcttagcgcg tggtggtagg cccccacctc gttcaacatc
      481 gtcgcaaccg ccatgaggac gtctgggtct cggctatagc gatcagtcaa cccctccaac
      541 tctcgcgcgg catccgaatc aagccccagc gtcttgagtt caatagctcg tcggtaggcc
      601 gactgctgct cgacttccac ccggctcgac accgagcctg tggccgtgcc actcatcggc
      661 gtgccgccgt tcgcaggggc ggtcgccgag attggttctg tcacgatgga ctccgacaag
      721 ggaagatcga ttcgttcgcg tgccagctgg caatagtagg tgtagacata tcgttgacag
      781 agctgtacat acacatcgcg agcataaggt tgctgggtca attcggctgc acgtgccatc
      841 caatacagtg cctgcggttc aaaatcgccg tcgtgctgat cggcgagcag gcgcagttcg
      901 tcacccgcct cacgatagcg cgccgtacgg taatacaccc aacccacacg ccaccgggcc
      961 tcggcgcgct gagacgccgg ctctccaacc tttgccacct gacggtaccg cacaatcgca
     1021 tcgtcaaagc gactctgatc ttccaaccaa atccccgcga acaaggtgat ctggcccttc
     1081 tgctccgcag aaagcaagga tgtcggcagc gtacgggcaa gatctaacaa cttgtccccc
     1141 tgcccctgtc ggagatagac acgggcgagc cagactgacg cctccaggga ccccacccct
     1201 ccgtctttta ccaacgcata gaacgtctct cgggcttggt catatagttt cagacggact
     1261 tgtgcgatac ctattttcag cttgacctcg gcgcgccggg gcgactttgg atcagccgca
     1321 agaaatttct tgaattcctc gattgcctcg gtatggaagg attgaccaag aaatacctga
     1381 gcccgggcaa gccgatcgct tgactgaacc acccaaggtt cgcccccaag atttgagacc
     1441 agtagcgctt ccgcttcctt cgcttcctga ctataggcaa atcggaccca gagttgattc
     1501 agtgtctcgc gacctttcgg tatgttgttg tctcgtagtt ggcaggcggc ccgccgaaga
     1561 aacgcctggg gagtccctgg ctccttatca tccaactcca ccgcccttgc aaaccacgct
     1621 gtcgcttcca gacaactcga cgcttgatac catgcttccc ctgctcgata cgcggccttg
     1681 gccaatagat acgagtcggg cactgcctgc ggaatgcctt cgaacatgtc cgccgcctgc
     1741 ttggcatctc cgaggttcaa cagcgcctcc ccagtccata gtcggatata atcgtccaag
     1801 atcggaaagt ctcgctgtga tgtgcggagg aattgaatgg ccacggcagg atttcgttca
     1861 aggagcaata cgccggacag taatcctgcg cgcttggccc agagtgtggc ggggaatcgc
     1921 tccatcaacc gttgtagccg actcagcttg agcgatagaa cttgctcttt attaagggcc
     1981 gtgccgagcc gttccttcgg cagtgcggca gcctgaaagc attcttcggc cgtctcacat
     2041 gcgtcgctgt cacttgatat cgacggaggg ggagaatccg cggacgattg gtcggagaac
     2101 agagtaaatg cgcagacgcc aataaatgcc gcgaaggaca gatttcgcag gacctgtcga
     2161 tgtagatagt cgtgcacccg tcatctccca ttagatctat accatatata gcagtgcgcc
     2221 cccacggttg ccagcagagc tgcacaagcc ctatcccttg ttccagtttt tgatgccatg
     2281 gtaggctgcc ctcatgcggt atcaaaaaga cactctccca tcatccacat ctcccatgaa
     2341 cctcctgtcc cttgatgaag tagggatgtg ccggctcgtg cagcaatttg gatggcctcg
     2401 ctaccgaacc ggtcaaatcc tacggtggct ctatcaacgt cgagcgaaag acatcaatca
     2461 gatgacggac ctcggccaag cggagcgaac agcattggct gcactggcaa cgattccacg
     2521 ggccgtaggt tgcacggtgt tccgttcaat tgatcacacg agaaaactga tactcaccct
     2581 caacgatgga ctctccgtcg aaacggtgat gattcccgat gaagaccgcc tcaccctctg
     2641 tgtgtcaacg caggtcggct gtacgctgga ttgtggattc tgtctcacgg gcacgatggg
     2701 attgaaacgt aacttgaagg cacatgaaat catggagcag gtgctgacag cccaagatcg
     2761 cctcgactcc ggtgaacgga tcaccaacct cgtctttatg gggatgggcg agcctttggc
     2821 caacatggag tccttgtcag aggccattcg acgactcacc aacaaaccgt ggggtctcgg
     2881 atggtcgcca agacgcatca cggtctcgac cgcaggcctg gcggcacgat tgaaagatgt
     2941 cgcaccactt ggcgtcaatc tcgccatctc gctgaatgcg acgacggatg accagcgcgc
     3001 aagcctgatg ccggccgtca atggtattgc ctcactcaaa acgctgctcg cggcctgccg
     3061 ccgctatcct ttacccccga ccagacggct caccttcgag tatgtgctgc tcggtggcgt
     3121 gaatgatcac gccgacgatg cgcgccgact catcaagctt gtccgaggaa tcgcgtgcaa
     3181 aatcaatctg attccattca atgaatttcc cggaagttct ttttgccgcc catcggaaga
     3241 caacattgca gcgtttcaag ccatcgtgcg acaggcaggc ctcgatgtgt ttgtccgcaa
     3301 aagccgggga cgcgacgtcc tcggcgcctg cggccaatta ggcaacctcg cacccgaata
     3361 tgccacacac gccttgacac aaattgaatc ccgttgctag catgtagagt gcttgccgcc
     3421 taaacatgac ctggctgtat tctcgacaca tcacgatgca tataggctgc ttgattgccg
     3481 caagcagtct tgcgttcgta ttcattgcgt catcggccgt cgccgcggaa cctcgtgagt
     3541 ttatcctctc caatgggatg aaagtcctgt tagtcgaagt tcccaaagcc ccggttgcca
     3601 ccgtccaagt ctggtacaaa gtcggctcac gcaatgaagt catgggccgt gcgggcttat
     3661 cccacatgct tgaacatatg atgttcaaag gcaccgcgaa ataccccaaa gggacatttt
     3721 cacgcctcat tcgaaagaat ggcggaacgg acaacgcctt cacgagccag gactttaccg
     3781 cctacttcga aaatctcgca gccgaccgtg tcgaactggc cctggagctc gaggcggatc
     3841 gaatgcaggg gctcatgctc gacatgagtg agttgacgac agagcgagaa gtggtaaagg
     3901 aagaacgacg tcttcggacg gaagacgacc cacaaggggc gctcgtcgaa gcgctcttcg
     3961 cccaagccta tctcagccat ccataccatt ggccggtgat cggctggttc agcgatctgg
     4021 acgcgatgac cctggatgac ctgcaacgac actacgacac ctattactca ccgaacaatg
     4081 cgaccctcgt cgtggtgggc gacatcaaag ccgacagcct gctcgccatg attaaacatc
     4141 tgttcgagcc gatcccacga ggacccgaac ccaagccgat cgccaccatg gaatcggaac
     4201 aaaagggtga gcgccggt
//
LOCUS       DATIPF010000247         3454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_931917, whole genome shotgun sequence.
ACCESSION   DATIPF010000247 DATIPF010000000
VERSION     DATIPF010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931917"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..640
                     /locus_tag="VLL06_06240"
     CDS             323..640
                     /locus_tag="VLL06_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30600.1"
                     /translation="MSSLPLLFKKEGLIEKHQVEGIDPSDRYFNRTVLVNRTPSGYAA
                     KVMYEALTVEGHSHPTIAAAVQELIELMQGFGFTKLRTRANFKGTKYLAEKETWLDYQ
                     DLA"
     gene            complement(657..1793)
                     /locus_tag="VLL06_06245"
     CDS             complement(657..1793)
                     /locus_tag="VLL06_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HSS30601.1"
                     /translation="MATSHVIQAALQSAVHDGVFPGAQLAVRLRGELQCLVVAGRLSS
                     EPPGLPVQPTTIYDLASLTKPLATVTSVLLLIQRAQVSLEDSVQEILPELEGATIGRV
                     TVRDLLTHRSGLPGWRPFYERLDARGHVSGLPGGDPFIKQQVLQMIRDEPLIYVCGER
                     SVYSDLGFILLGFLVERLSGMALDLWCDDAIVRPLEADPLMFCPTAGRVQPGIGRPAV
                     DASRIAPTEQDEWRHRLLCGEVHDENAAVMGGVAGHAGLFGTAESVLVVSGAWLHGYH
                     GRESMLDEKLVRQFTARQESAARSSWALGWDTPSSPSSAGSRFSERSFGHLGYTGTSL
                     WIDPLCELEVVLLSNRVHPSRKNEKIKAFRPSIHDLVYREFVSA"
     gene            complement(1893..2423)
                     /locus_tag="VLL06_06250"
     CDS             complement(1893..2423)
                     /locus_tag="VLL06_06250"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HSS30602.1"
                     /translation="MTMGDAMRITPLDIQQMVFKVGMRGYDKEEVNRFLEELAQTVES
                     LNRDNAVQREKIMFLEQQLAELKRTEATLSSTLLSAQSLADDVKRNAQREAELVIKEA
                     ELKAGELIRQARVELTDTQRELLSLQKQRLLMIERLRASLRMFERLFEVEEQEAFQDA
                     ANSSAEKLEGESSPAL"
     gene            complement(2425..2721)
                     /locus_tag="VLL06_06255"
     CDS             complement(2425..2721)
                     /locus_tag="VLL06_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HSS30603.1"
                     /translation="MFVAGNALSGIATILDYVLWLYMWVIIARALISWVNPDPWNPIV
                     QFLNRATEPVLAPIRRLIGLRIGMDLSPIIAILIITFLQIAVVQSLKEFAMRMN"
     gene            complement(2721..>3454)
                     /gene="proC"
                     /locus_tag="VLL06_06260"
     CDS             complement(2721..>3454)
                     /gene="proC"
                     /locus_tag="VLL06_06260"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HSS30604.1"
                     /translation="QVCSAETIWATDPIAERRDRLKGQFGVQVGSGNPEAVTWAEVVV
                     LAVKPQILPAILRELGPILAHPLVISIVAGVTIRTIAEQMGGGTRVVRAMPNTPALVR
                     EGMTALAMGVGVSDKDVHLARTIFETVGLVVAVEERLMDAVTGLSGSGPAYVFQAIEA
                     LADGGVMMGLPRQTAELLAAQTVLGAARLVLESGVHPAQLKDRVASPGGTTIAGLHQL
                     EQGGFRAALMAAVEAATTRSKELGR"
BASE COUNT          826 a         1055 c          877 g          696 t
ORIGIN      
        1 gccccgagcc gtgtatactg attcttcact cgggcgattg caccatcaag tgcatcagtg
       61 agtccggcca cgatcagcac gatgagcgcc tgatcatatt ttccgtagat cagtaaccca
      121 atgtagaagg gaatgaggag aatacggagg atggtcaggc tgttggggat attcatatct
      181 tccggaacat agcggcctac cgcagcgggg gggaatgata ggaggccgtt acacagttgt
      241 caacggcttg cagtcggcac ggccccaccc tataatcaat cgggatcctg aaccattcca
      301 gttacgtgtc atggaggtcc tcgtgagttc ccttccgttg ctctttaaaa aagaaggcct
      361 gattgaaaaa catcaggtcg aaggaatcga cccgagcgat cgatatttca atcgcacagt
      421 cctcgtcaat cgaacaccct ccggctacgc cgccaaggtc atgtatgaag ccctgacggt
      481 tgaagggcac tcgcatccga ccatcgctgc tgcggtacaa gaactcatcg agttgatgca
      541 gggattcggc ttcacaaaat tgagaacccg cgccaatttc aaaggcacga agtatctcgc
      601 cgaaaaagaa acctggctcg actaccagga cctcgcgtag actcaacaat cacgaatcac
      661 gcagacacaa actcccgata gaccaggtcg tgaatagacg ggcggaaggc cttaatcttc
      721 tcgttcttcc ggctcgggtg tacccgattg gataacaaca ccacctccaa ttcacacagc
      781 ggatcaatcc acagcgatgt cccggtgtaa ccgagatgcc cgaacgaccg ctcggagaaa
      841 cgggatcccg ctgaggacgg cgatgacgga gtgtcccatc ccaatgccca actcgatcgc
      901 gcggcagatt cctggcgggc cgtaaattgc ctgacaagtt tctcatccag cattgactct
      961 cttccatgat agccgtgcag ccaggctccg gagacgacca gcaccgattc ggcagtccca
     1021 aacagcccgg catggcctgc gacaccaccc atcaccgcgg cattctcgtc gtgcacctcc
     1081 ccacataaga ggcgatggcg ccactcgtcc tgttcggtcg gagcgatacg tgatgcatcg
     1141 accgccgggc ggccaatccc aggttggact cgacctgccg tagggcaaaa catcagtgga
     1201 tcagcctcta gtggtcggac gatggcatcg tcacaccaga gatccagtgc catcccactc
     1261 agtcgctcca ccagaaatcc cagcaatatg aatccaaggt cgctatagac acttcgctct
     1321 ccgcatacat agatcaatgg ttcgtcgcga atcatctgca agacttgttg tttgatgaat
     1381 ggatcacccc cagggagccc cgagacgtga cctcgtgcgt caagccgctc atagaacggc
     1441 cgccatccgg gcagacccga acgatgggtt aagaggtcgc gcaccgttac ccgaccgatc
     1501 gtcgcccctt caagttcagg taatatctct tgcacggaat cttcaagaga aacctgagcg
     1561 cgctgaatga ggagcaagac ggatgtgacc gtcgccagcg gtttcgtgag agaggcgagg
     1621 tcataaatcg tcgtgggttg gacgggaagg cccggaggct ctgacgacaa tcgcccggct
     1681 acaaccagac attgcaactc gccacgcaga cgtaccgcca gttgggcacc gggaaagaca
     1741 ccgtcgtgaa cggctgattg cagggccgcc tggatgacgt gactagttgc cataggcgaa
     1801 agacctgtca tggggcggga aagttgctgc tgggtggcgc ggagcaaatc aagcgccgaa
     1861 tcagtgcaca agagccgacg agatccaaca cgtcataagg ccggacttga ttctccttcg
     1921 agcttttccg ccgatgagtt cgcggcgtct tggaatgctt cctgctcttc cacttcaaac
     1981 agacgctcga acatccgcaa cgacgcgcgc aaccgttcaa tcatcaacaa tcgctgtttc
     2041 tgaagtgaca agagttcgcg ctgcgtatcg gtcagttcaa ccctggcctg acggatcaac
     2101 tcgccagcct tcagctcggc ctccttgata acgagctccg cctctcgctg agcattccgc
     2161 ttcacatcat ctgccagcga ttgagcagac agcagggtac tcgacaatgt ggcttctgtc
     2221 cgtttcaact cagcgagctg ttgttccaga aacatgatct tttcccgctg aacggcattg
     2281 tctcgattca atgactcgac cgtctgcgcc aattcttcga ggaagcgatt gacctcttct
     2341 ttgtcatacc cgcgcatgcc gaccttgaac accatttgct ggatatcgag cggtgtaatt
     2401 ctcatggcat cccccatggt catctcagtt catcctcatg gcaaattctt tcagcgactg
     2461 cacaacggcg atttgcagga acgtgatgat caatatcgca atgatcggcg acagatccat
     2521 tcctatccgt aatccgatca atcgacgaat cggcgccaag acgggttcgg tcgctcgatt
     2581 gagaaattgc acaatggggt tccatggatc cgggttgacc cacgaaatca acgcacgagc
     2641 aatgatcacc cacatataaa gccataacac ataatcaagg atggtcgcga tacccgaaag
     2701 agcattgccc gcgacaaaca tcagcgcccc agttctttcg atcgcgtggt ggctgcttcg
     2761 actgccgcca tcagtgcagc acggaatccc ccctgttcga gctgatgaag ccctgcgatg
     2821 gtcgtgcctc caggagacgc cacgcgatct tttagttgag cggggtgcac ccctgactcg
     2881 agcaccaatc gagccgcgcc gagcaccgtc tgggctgcaa ggagttcggc tgtctgtctc
     2941 ggcaagccca tcatcacccc cccatccgcg agcgcttcaa tagcctgaaa tacatacgcc
     3001 ggcccactgc cactcaatcc ggttacggca tccattaatc gttcctcaac cgccaccacc
     3061 aggccgaccg tttcgaatat cgttcgagcc aggtgaacat ccttgtctga aaccccaaca
     3121 cccatcgcaa gagcggtcat gccctcccgg accaatgctg gagtattcgg catcgcacgg
     3181 accactcgcg tccccccgcc catctgttct gcgattgtcc gaatcgtcac ccccgccaca
     3241 atagaaatga ccaggggatg cgccaaaatc gggccaagct ctctcagtat ggcaggaaga
     3301 atctgaggct tgaccgccaa gacaaccact tcggcccagg tgacagcctc agggttgccg
     3361 gatccaacct gaacaccaaa ctgacccttt aagcgatcgc ggcgctcggc aatgggatct
     3421 gtcgcccata tcgtctcagc agaacacacc tgcc
//
LOCUS       DATIPF010000248         2889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_489326, whole genome shotgun sequence.
ACCESSION   DATIPF010000248 DATIPF010000000
VERSION     DATIPF010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489326"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1058
                     /locus_tag="VLL06_06265"
     CDS             150..1058
                     /locus_tag="VLL06_06265"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HSS30605.1"
                     /translation="MRQLSSMMTSEGWIDVQIRTMVDAAELLGRLADPVIQGGWEENG
                     VIHLYWPKQQWSLDAHVHLSRTLLGLDPDLSTERDIRVEELPDQDWNRQWAQSVRPIR
                     IGRRILIRPSWETVALQAQDIEIVLDPKQAFGTGHHATTRMLLEWLEDLIGGGEFVLD
                     VGAGSGILAMVALRLGAASALGVECDPVAVDCARDYAAGNGFGDNLEFRCGTLEDIDR
                     HGALRPDLVLANLDCQTLLLLCDELARYVSRGARLLLSGILLDQESEIVEAFSKVGAM
                     LLQRREQEGWVALELLMAESCEGAGA"
     gene            1055..1546
                     /locus_tag="VLL06_06270"
     CDS             1055..1546
                     /locus_tag="VLL06_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HSS30606.1"
                     /translation="MTAPGRPPYPHVHQINVSDGGVPKLPVLEAVIATTGVLGDRQRN
                     LKFHGGPDRAVCLFSQELIERLQDEGHSIEAGSYGENLTLAGLDWEKLKLGARLQIGP
                     DVRVELMSYTVPCDKNARWFKDGDFKRVSQKINPGWSRLYARVLVEGVVRPGDAVVIE
                     QTD"
     gene            1624..2283
                     /locus_tag="VLL06_06275"
     CDS             1624..2283
                     /locus_tag="VLL06_06275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSS30607.1"
                     /translation="MKRTLEPELMDDPAQALAYSRADFEKENQGFIDRFREYFPEFTE
                     GHILDLGCGPGDIPVRFARALPLCRITGVDASEPMIGLAGVVVKQAGLVDRIAFRCER
                     FQAVSLAEPADAAVSNSLLHHVPNPLQLWYRLRQVVKSGSPVLVMDLLRPNSPEEAQA
                     IVDRYAAQEPEILRRDFYRSLLASFTEDEVAAQLAEMNMSRLMIDVVDDRHWVVSGII
                     H"
     gene            2502..>2889
                     /locus_tag="VLL06_06280"
     CDS             2502..>2889
                     /locus_tag="VLL06_06280"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSS30608.1"
                     /translation="MQFSLGISFLLLLLMGTLASPEFSQAQLELLRKDRPVEQTAPAE
                     TPMVEIPAGEFAMGLDGVQALEDERPMHRVWLDRFSMDLHEVSTAQYAEFLAATHRPA
                     PWQWNEGELSQTRNRPVIGADWSDADA"
BASE COUNT          582 a          739 c          920 g          648 t
ORIGIN      
        1 cgccagcgcg tgaaggtgga actcgaggca gtggccaggg caatgaggcg gtcgagtctc
       61 gcgaccaggc gtgtcagcga atgggctttc ggtacgggca tcacggaccg tttctggagc
      121 atagcgccga ccttactgga agggggagag tgcgtcaact ctcgtccatg atgacgtctg
      181 agggatggat cgatgtccag attcgaacca tggttgatgc ggcagagtta ctcggcaggc
      241 tggctgatcc cgtgattcaa ggcggatggg aggagaacgg agtgatccat ctctattggc
      301 cgaaacaaca gtggagtctg gacgcacatg ttcatctgag ccgtacgttg ctgggactcg
      361 atccagacct ctcgaccgag cgcgatattc gtgttgagga attgccggat caggattgga
      421 atcggcagtg ggctcagtcg gtcaggccga ttcggatcgg tcggcggatt ctgatcaggc
      481 ctagttggga gactgtggca ctccaggctc aggacatcga gatcgtgctc gatccgaagc
      541 aggcattcgg cacgggccac catgcgacga ctcgaatgtt attggaatgg cttgaagacc
      601 tcattggcgg cggcgagttc gtgctggatg tgggggcggg gagcggcatt ctggcgatgg
      661 ttgcattgcg gcttggagcc gcttcggccc ttggcgtgga atgcgatcct gtggcggtgg
      721 attgcgcacg agactatgcc gcagggaacg gatttggaga caatctcgaa ttccgatgcg
      781 gaacgttgga ggatatcgat cggcacggcg cattgcgacc ggatctggtt cttgccaatc
      841 tcgattgcca gacgctgttg ttgttatgcg atgaactggc gcggtacgtc agccgcggtg
      901 cgaggctctt gctgtcgggc attctgttag atcaagagag cgagatcgtc gaggcgttct
      961 ccaaggtcgg cgccatgctg ttacaacggc gcgaacagga gggttgggta gcgttggaat
     1021 tgctgatggc ggaatcctgc gaaggagccg gtgcatgacc gcaccaggac ggccacccta
     1081 tccccacgtg catcagatta atgtgtcaga cggaggcgtg ccgaaacttc cggtgctgga
     1141 ggcagtcatc gcgaccaccg gagtcttggg tgaccggcaa cggaatctca aattccacgg
     1201 cgggcccgat cgggccgtct gtctgttctc gcaagaactc atcgagcgac tgcaggacga
     1261 aggccattcg atcgaggcgg gttcttacgg agaaaacctg acgctcgccg ggcttgactg
     1321 ggagaaactg aagttgggtg cccgactcca gatcggtccg gacgtccggg tcgagctcat
     1381 gagctacacg gttccctgtg ataagaatgc ccggtggttc aaggatggtg atttcaagcg
     1441 ggtctcacag aaaataaatc caggatggag tcggctctat gcccgagtgc ttgttgaagg
     1501 agttgtacgg ccaggggacg cagtggtcat tgaacaaaca gactagctct cagccatcag
     1561 cgttggccgc ggaatcggat gactgcggat ggctgaaggc tggttgctga aagcaaggaa
     1621 tctatgaaac gcacacttga gcctgaactg atggatgatc ccgcgcaggc gctcgcatat
     1681 tcacgggcag atttcgaaaa ggagaaccaa ggatttatcg atcgcttccg cgaatacttt
     1741 ccggaattca ccgaggggca tattctcgac ttggggtgtg gccctggcga tattcccgtc
     1801 cggtttgcgc gggcgcttcc cctctgtcgt atcaccggcg ttgatgcatc ggagccgatg
     1861 attggactgg ctggtgtggt agtgaaacag gcaggtctgg tcgaccggat tgcatttcgc
     1921 tgcgagcggt ttcaggctgt gtcgctggcc gaacctgccg acgccgcagt gtccaacagt
     1981 ctgttgcatc atgtgcccaa tcccttgcag ctttggtatc ggcttcggca agtagtgaag
     2041 tcgggttccc ctgtgctcgt aatggatcta ctcagaccga actcgcctga agaggcacag
     2101 gccattgtgg atcgctatgc ggcacaggaa ccggagattt tgcgccgcga tttttaccgc
     2161 tccctgctgg catcgttcac cgaagatgaa gtggcggcgc agttggcgga gatgaatatg
     2221 agccgattga tgattgatgt ggtggatgat cggcattggg ttgtcagcgg catcatccac
     2281 taaccggtag gatgctgaaa acgatcccca acttcgttct cgtctcgtca gaatcctcag
     2341 cgtacctcag agggtacgcc tccggttctg actcgcctgc ggcctcgttg gatgatcgtt
     2401 ttgagcatcc tacccacgct gtaccggttc cctcttgagc ctcaacaccg atctcttatg
     2461 accctcgcac gatggtgatt gcttgtgtat gctactgcgt catgcagttc tcgctcggaa
     2521 tctcattcct tctcctgctc ttaatgggga cgcttgcaag tcctgagttc tcccaagccc
     2581 agcttgaact cctgcgaaaa gatagaccgg tcgaacaaac ggcaccagct gaaactccga
     2641 tggtggagat tccggccggt gagtttgcta tgggattgga tggggtgcag gcgttggagg
     2701 atgaacgtcc catgcaccgg gtgtggctcg atcgcttttc gatggatctt catgaggtgt
     2761 cgaccgcgca atacgcagag tttctcgctg ccacacaccg tccagcgccc tggcaatgga
     2821 acgaaggaga actgtcgcag actcgcaacc gtccagtgat tggcgcggac tggtcggacg
     2881 ctgatgcct
//
LOCUS       DATIPF010000249         3940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1724108, whole genome shotgun sequence.
ACCESSION   DATIPF010000249 DATIPF010000000
VERSION     DATIPF010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724108"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..829
                     /locus_tag="VLL06_06285"
     CDS             227..829
                     /locus_tag="VLL06_06285"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSS30609.1"
                     /translation="MDKAPRLATSTIDPSLLARVAKGDQQAYSQLYDHSSTLLFTLAV
                     RILGNPEEAAELLQDVYLEVWRKVSRYDVGRGTPIAWLVTLTKSRAIDRLRARTVRGY
                     RATSSLEAGQAAQVADLGPSPFETQADQELRIAVGAAMAGLPQAQQQAIELAYYEGLS
                     HAEIAERLNQPLGTVKTRIKLGMSKLRDGLQQWRTHGDLP"
     gene            826..1842
                     /locus_tag="VLL06_06290"
     CDS             826..1842
                     /locus_tag="VLL06_06290"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HSS30610.1"
                     /translation="MTHEELEAAVPLYAAGALDRVERQALEAHLLSGCTSCHSALKDY
                     QSVASLLPFSLSPTKPPRSLKAKIMTERNPAPIPADATANEPVRPSLEPGEWMNHLFP
                     PVAPARSLSLPWALGLAALLIVTVGGYLAWTVSTQRSADTSNIMQLEASLKEKTEKVA
                     GMQREVNDQAKVLDELRNALLQRTNEAAEVKEQLAQREEELEESRVQLSQRSVGRIVG
                     TPQDEFATLLRIPNVKAVSLTGSDMAKRAVGFLLYDPRTQNAWLYSVNLPESPTGTTY
                     QLWAIYDKPVSIGTFQMDRGDTAHLLMKKMPTFSDARTFAVSLEPSGGRPQPTGPIFL
                     LSRS"
     gene            1847..2332
                     /gene="tadA"
                     /locus_tag="VLL06_06295"
     CDS             1847..2332
                     /gene="tadA"
                     /locus_tag="VLL06_06295"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HSS30611.1"
                     /translation="MGTVQTDEHHQDVAFMRIALEEAARAPAIGEVPIAAVIVRGSQI
                     LAQAHNCRELWQDPTAHAEVIAIRAAANTLGTWRVTDTTLYVTVEPCSMCIGAIILAR
                     IPRIVFGAWDLKAGACGSVFNLPSEPRLNHRVAVTGGVLEQESQALIQKFFKQLRETP
                     L"
     gene            complement(2438..3166)
                     /locus_tag="VLL06_06300"
     CDS             complement(2438..3166)
                     /locus_tag="VLL06_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HSS30612.1"
                     /translation="MAGWSTPIAMEDRTIHVWGFSLEGSLSFLEQCRSSLSEDERGRA
                     ARFIRQQDHVRFVLAHGGLRAVLSRYLGVGSDAVAMNRNEAGKPSLVRIRKDQPTISF
                     NMSHAHGRALIAVSKGQEIGVDLERVRSDVEAEKLSQRYFAPSEHATIMRLAQEQRAA
                     RFFRYWVAKEAVLKAQGVGLRGLSQCEVLMSADGIGAEALVPTGVPFQDDWRIRFLSC
                     GEGWEAAVASHGVDWVAQCGVAGC"
     gene            3403..3492
                     /locus_tag="VLL06_06305"
     tRNA            3403..3492
                     /locus_tag="VLL06_06305"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3437..3439,aa:Ser,seq:cga)
     gene            complement(3565..>3940)
                     /locus_tag="VLL06_06310"
     CDS             complement(3565..>3940)
                     /locus_tag="VLL06_06310"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HSS30613.1"
                     /translation="LAAALPSLRPALSRIVSRLWDLFPIIRDHYYHPAFGGSYSIKSV
                     LPAIVPSLAYDDLAIQEGGHAASQYYRMVFVETDWVERATIEEALLRYCARDTLAMVE
                     LRRVLMEKAQMKSGTGWSGLSG"
BASE COUNT          910 a         1200 c         1046 g          784 t
ORIGIN      
        1 ctggggcgca cggtgcgacg aataaggagc accaagtctg tgcgcgccgc cgagttggtg
       61 aggtggccag gtccccgttg aggatctgat cgatgcgaga acgccgctgg aggacttttt
      121 cagcattctg ctgaaacaca catccttttg cccgttctct ccgtaggatg ctatagtcgt
      181 ggcggacaag aattgacgcc gacgtttcct cgcagcccgc tctcgtatgg ataaagctcc
      241 acgactcgcc acctcaacga tcgatccaag cttgctcgct cgtgtcgcca aaggcgatca
      301 gcaggcctac agccaactct atgatcattc gagtacgctc ttgttcaccc tggcagtcag
      361 gattttgggc aatcccgagg aagcagcgga gctcttgcag gatgtctatt tggaagtctg
      421 gcggaaggtg tcccgctatg acgtcgggcg tggaaccccg atcgcatggt tggtcactct
      481 caccaagagt cgggccatcg accggctacg ggccaggacc gtacgaggct atcgggccac
      541 gagttccctg gaagcaggac aggctgcgca ggtagccgac ttgggaccaa gcccttttga
      601 gacgcaggcc gatcaagaac tgcgcatcgc ggtcggggca gccatggcgg gattaccgca
      661 ggcacagcag caagcgattg aactcgcata ttatgaagga ctctctcatg ccgagatcgc
      721 cgagcggttg aaccaaccgc tcgggaccgt caagacacgg atcaaactcg ggatgtccaa
      781 attgcgggat ggccttcaac agtggcggac tcatggtgat ctcccatgac gcatgaagaa
      841 ctcgaagcag cggttccctt gtatgctgct ggcgcgctgg accgggttga gcgacaagcg
      901 ctcgaagccc acttactctc cggctgcacg tcctgtcaca gtgccctgaa ggactatcaa
      961 tccgtcgcct cactcctccc attcagcctc agcccgacca agcctccgcg ctccctcaaa
     1021 gccaagatca tgaccgaacg aaaccccgca ccgattccag cggacgcgac cgcgaacgaa
     1081 ccggtccggc cgagtcttga accgggcgaa tggatgaacc acctcttccc tcccgtcgcc
     1141 cctgctcgat cgttatcact gccatgggcc cttggcttgg ccgccctcct catcgtgacg
     1201 gtcggcggat atctcgcgtg gactgtgtcg acacaacgtt ccgccgacac atcgaacatc
     1261 atgcagcttg aagcatcgct caaggagaag acggaaaagg ttgccggtat gcaacgtgag
     1321 gtcaacgacc aagccaaagt cctcgacgaa ttgcgcaatg cactgctgca acgaacgaac
     1381 gaagcggcgg aggtgaagga acaactggcc cagcgcgaag aggaactgga agagtcgcgt
     1441 gtgcaattaa gtcagcgaag cgtcggtcgc atcgttggga ctccgcaaga cgaattcgcc
     1501 acactcctgc gaattcccaa cgtcaaagcc gtctctctca ccggatcgga tatggccaaa
     1561 cgagccgtgg gattcctgct gtacgatcct cgaactcaaa acgcctggct ctactccgtg
     1621 aacctgccgg agagtcccac tggcacgacc tatcagttgt gggccatcta cgacaaacct
     1681 gtaagcattg gcacgtttca gatggatcga ggagacaccg ctcacctcct gatgaaaaag
     1741 atgccaacct tctcggatgc gaggaccttt gccgttagcc ttgagccctc aggcggccgt
     1801 ccgcagccga ccggaccgat ctttctcctc agccgatctt aacaagatgg gcaccgtgca
     1861 gacagatgaa caccatcagg atgtggcctt catgcgaatc gcactggagg aggccgcgcg
     1921 cgcgccagcg atcggtgagg tgccgattgc ggcggtgatt gtgcgtggaa gtcagattct
     1981 ggcccaggca cacaattgcc gggaactctg gcaggacccc acggcccatg cagaggtcat
     2041 tgccatccgc gccgcagcga acacacttgg cacgtggcga gtgaccgata cgaccctcta
     2101 cgtgaccgtc gaaccctgct cgatgtgtat cggtgcaatt atcctcgcga ggattccccg
     2161 aatcgtcttt ggagcatggg acctgaaagc cggcgcctgt ggatcggtgt tcaacctgcc
     2221 ctctgaaccg aggttgaatc accgggttgc tgtaactggc ggggtcctcg aacaggagag
     2281 tcaagctctc attcaaaagt ttttcaaaca gctccgagaa acgccgctct agcccggtct
     2341 gtctagtcca tctggtgtgt ctggtctatc tgttcgtttc gttcaaccgt tcacacgaga
     2401 tagacagaac agaccgaata gaccagacag accattttca gcatcctgcg acaccgcact
     2461 gcgcgaccca gtcaacccca tgcgaggcta cggctgcttc ccagccttct ccgcaagaga
     2521 ggaaacgaat cctccaatcg tcttggaacg gaacgccggt cggaaccaga gcctccgcgc
     2581 cgattccatc cgctgacatg agaacttcgc actgactcaa gccccggagt ccgacacctt
     2641 gcgctttgag caccgcctcc tttgcgaccc agtagcggaa gaaccttgct gcacgctgct
     2701 cctgagccaa ccgcatgatc gtggcatgtt cggatggagc aaagtaacgt tgggagagtt
     2761 tttccgcctc tacgtccgaa cgaactcgtt cgagatcaac tccaatctct tgcccctttg
     2821 aaaccgcgat gagggcacgg ccatgcgcat gcgacatatt aaaagagatc gtgggttgat
     2881 cttttctgat tctcaccagg gaaggcttac ctgcctcgtt gcggttcatc gctaccgcat
     2941 cagaaccgac cccaagatac cggctgagca cggctctcaa acccccgtga gcaagcacaa
     3001 atcgaacatg atcttgctga cggataaacc tggccgccct ccctcgttca tcttcgctca
     3061 acgaggaacg gcattgctcc agaaaagaaa ggcttccctc cagagagaag ccccagacat
     3121 gaatggttcg atcctccatc gcaatcgggg ttgaccaacc agccattgcg atcgactcaa
     3181 tgggcggaaa aaccatgact ggctcaccta ttcccgtacc tatattcaac agtaccctcc
     3241 tctagccccc atcaacctcg tcggcgcgac aatcgtactg gctcttcgat cgaaaagaaa
     3301 accgattgat aaatcacacc ccccaaggta agctgccccc acagtcgatc atagcgataa
     3361 tagatttccc cttaccctgc cgtgggatga gttctatcat gcggagaggt gcgagagtgg
     3421 ccgaatcggg cagtctcgaa aactgctgtc ctggcaacgg gaccgtgggt tcgatcccca
     3481 ccctctccgc caaactaagc tcgctgttgt ttggtttgtc tggtgtattt tgtttgtgag
     3541 atacaaagaa cggaaaaaac cagtctaacc agataaacca gaccaaccag tcccgctttt
     3601 catctgcgcc ttttccatta acacccgcct gagctcgacc atcgccaagg tatcccgcgc
     3661 gcagtatcgc aacagggcct cttcaatcgt cgcccgttcg acccaatccg tttcgacaaa
     3721 gaccatacgg tagtactgac tggctgcatg gcccccctcc tgaatcgcca aatcatcata
     3781 ggccagtgag ggaactatgg caggcagtac agatttaatg gagtaggacc cgccgaacgc
     3841 aggatggtaa taatggtctc gtatgatcgg aaagagatcc cagagtcggc tcacgatacg
     3901 cgaaagcgct ggcctgagag acggaagcgc ggcggcgagc
//
LOCUS       DATIPF010000250         7373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_489910, whole genome shotgun sequence.
ACCESSION   DATIPF010000250 DATIPF010000000
VERSION     DATIPF010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7373
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489910"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1015
                     /locus_tag="VLL06_06315"
     CDS             170..1015
                     /locus_tag="VLL06_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HSS30614.1"
                     /translation="MTKTSAQSTPAYRLAVRVGTLCAVGFAAVLFVGCGELEPMREPE
                     VVELELTTDTLKASVREAQRTVTELRADLEARRRELAEAQVARAQLEGRVREAERRVI
                     EARQVIELQREELAAARTERERVFRSSLQLQSQMKQLRRQRVNTGKLGSAQGAQEATP
                     LPSSGATRKSQKAIQVPVQQTQPSEFLETPQMVAAPASARQVPADREPSSGVEQPRTP
                     MRHVSVKPGDTLWRIAQNNRVSVEYLRTFNQLTDNRIVIGQALWLPDDRPMSEVDADK
                     GSATP"
     gene            1060..2271
                     /locus_tag="VLL06_06320"
     CDS             1060..2271
                     /locus_tag="VLL06_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSS30615.1"
                     /translation="MPVFAYRVARPDGSTLDGQIEGEEEQLVRAKLEGQGFLVFRLQR
                     RGAATTGLGLDWHAFGKLPLQEFLVFNQELLALVKAGLPVLRVWDLLIERASHSGFQQ
                     ALRTVRQDIRGGASASEALAKHPIHFSELYIATIKAGEQSGNLAEVLQRFIAYLKLMI
                     GLRQKVSKALAYPAFLVMVGISVIGFLLTYVVPTFVSVYAESSKSLPVATQLLLDVVA
                     GGNVYLLPGLVGLAVLVLVGRAYYVTPAGRLVVDQLLLRVPVLGPIFVKHYTVQLTRT
                     LATILAGGTPLVDALSIARGAISNRYVSFGVAGAVGEIREGTTLAAALDRPKVFPKLA
                     VEMLSVGEETGSLPAMLHDVAEFYEGDLDLRLTQLTTWIEPALLLIMGILVGAIVIIM
                     YLPVFQMAGAV"
     gene            2334..4061
                     /locus_tag="VLL06_06325"
     CDS             2334..4061
                     /locus_tag="VLL06_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HSS30616.1"
                     /translation="MARTVTRPTLADVLIQQGVLPKQTLDQVLGRLDGVTAALGQTLV
                     GEGIISEDQLARALAAQFDLPCDLLTEFRVDQSFYNSMSVKLMQRHPFIPIAEHQGRL
                     TIAISDPQNLLALDELELLLGRPLDLVVSSRSAILAALERSEGSSQALRELEAEYRSV
                     LVKEDERGEEVFTIDHEGEDQSPAVKLLDSILLSAMQRRASDIHIEAADRATKVKLRV
                     DGILVPAMEPLDIRLHAPLVSRLKVMSDLDIAERRVPQDGSFRMRLERKTVDFRVSIL
                     PSVFGESVVIRILDREAITTGVAALRLERLGFNVEDLKRFRRAITRPYGMVLVTGPTG
                     SGKTTTLYAAITEMNIKEDKLITIEDPVEYQLSGVVQIPVNEKKGLTFARGLRSILRH
                     DPDKIMVGEIRDAETAQIAIQSSLTGHLVLTTVHANNVFDVIGRFASMGIDAYNFLAA
                     LNCVLAQRLVRMVCLGCRVAVKLDRALAEESGLDFEEYKDVPFYEGKGCQECHGTGYR
                     GRKCITEFLDLNDEIKEMILAERPLSEVRYRAVTSGMITLRQSALRKVLEGSTTLREI
                     NRVTFSEEG"
     gene            4065..5066
                     /locus_tag="VLL06_06330"
     CDS             4065..5066
                     /locus_tag="VLL06_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30617.1"
                     /translation="MWGLSTSRPQHCLKIGAQALVWGEAVRTWRGRYRYHCVLSPTPA
                     GAIKLSPMDLNLTDMSTVESRLRSLAGPSRDIRFAGRAWLSDVPRPIVLLLSDLSVRA
                     TVLQLEQFPAQVEEQEALIRWRLGQEQLLPMTGAKIVWQGFPSRGAGDERSHAIFVVA
                     IQETILKQYESLCESVGLLPQEVGVTSFRLFNLWLRAAGGRKRLGHDLFWVSISDGGL
                     TCFVMQGGRLVFVRTKLLIGERLQGEEDLEEDLVNTIAEECAVSLRTCLEHHPGLNVT
                     EIVLASDSPFPTLEQALKRELGLSTERLDWEAVKRLGWTHDGGSTSWATLPVVAGVM"
     gene            5070..5699
                     /locus_tag="VLL06_06335"
     CDS             5070..5699
                     /locus_tag="VLL06_06335"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HSS30618.1"
                     /translation="MAPERTWLRALAGTLSSFMVIPGGARYFHIELSSRYRWYLAPAR
                     LMLAALSWVIGASIFLSVAQTVITMRDLRDMQARIDHVRMQDRQLVAEAQQEGIDLSG
                     PSLQQLSAEVGLANQLLTKRNFSWTQFLSALEAAIPQHVSIQSIRLDPGSAVVHMIGL
                     AATVEDVTALTVTLQDHTAFHDPVLGQHRVGPNGLVEFDLTLRYRQPGV"
     gene            5706..6266
                     /gene="pilO"
                     /locus_tag="VLL06_06340"
     CDS             5706..6266
                     /gene="pilO"
                     /locus_tag="VLL06_06340"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HSS30619.1"
                     /translation="MTDFIFQQLRKPYGILIPWFALVMLLAGVLWAVHTVGFDGAEQN
                     RAKLESEWNQARQDLKHHKEAQKARKDLAQVWSVLPVERDFAPLALGITEEAKRDRVI
                     LPALSYKTEPTAVANTSKGLLQGTMTGRYEDLRRFLYDVETAEELVYIEDLELVQSDA
                     QENQKLTFNIKIATYLRGDSGKAAVQ"
     gene            6276..6860
                     /locus_tag="VLL06_06345"
     CDS             6276..6860
                     /locus_tag="VLL06_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30620.1"
                     /translation="MMTTNQKKAATLATLGLLWGGIVAWEWQTMEEPVRVPLANVSGP
                     VSTSRAARGTGEGLHVQLDLLAAARTQREMTFTPPRNIFAPPRPNDPMTTHANQSEFD
                     PGLDQTLRQQEALAGLTQFHYLGFVRMEEGWRNKGELAVLTKNDDLHVVRTGETVEDH
                     VVVKAITQESVTLQDRQLRVEHTVLLSEEAPVQP"
     gene            7119..>7373
                     /locus_tag="VLL06_06350"
     CDS             7119..>7373
                     /locus_tag="VLL06_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30621.1"
                     /translation="MTYLMLMFAIVLIGIATTAAAKQWKAIVQRELEADLLAKGIEIQ
                     NALALYSATIKAGRVMPGEVYPQTLADLTRLPKPFLRKVYL"
BASE COUNT         1578 a         1851 c         2278 g         1666 t
ORIGIN      
        1 gaggaatccc aaaggatcct ttcaccggtg cgacgaccac gtggcaagag attttggatc
       61 ctatcgagaa aggcgtgatc gatgtctttt caggcgctga atttgtgggg acggacggca
      121 ccccatataa tcgttggtag cgaaatccat gctcaatgtt cacgatagtg tgacaaagac
      181 ctcagcccaa tcgactcctg cataccgttt ggcagttcga gttgggactc tctgtgcagt
      241 cggcttcgcc gcggtcttgt ttgtaggatg tggtgaactt gagccgatgc gtgagccgga
      301 agtggttgag cttgagctga ctacagacac gttaaaagcg tcggtgcgcg aggcgcagcg
      361 gacggtgacc gagttgcggg ctgaccttga agcacgccgt agggaattgg cagaagctca
      421 ggtcgctcga gcgcagctgg aaggacgggt cagggaggcg gaacggcggg tgattgaggc
      481 ccggcaggtg atcgagttgc aacgggaaga gctggccgcg gcgcggacgg agcgggagcg
      541 agtgtttcga agcagtctcc aattacagag ccagatgaag cagcttcgga ggcaacgcgt
      601 caacacgggg aagttgggtt ccgctcaggg tgcacaggag gcgaccccat taccttctag
      661 cggtgcgacg cgcaagagcc agaaggctat acaggttcca gtacaacaga ctcagccttc
      721 agagtttctg gaaacacctc agatggtagc ggctccagct tcggctaggc aggtgccggc
      781 agaccgagaa ccctcgtcag gtgttgagca gccgaggact cccatgcgac atgtttctgt
      841 gaaacccggt gatacgctgt ggcgcatagc gcagaataat cgtgtctccg tggaatatct
      901 gcggacgttt aatcaattga ccgacaatag gattgtcatt ggccaggcgc tgtggctacc
      961 ggatgatcgg cccatgtctg aggtcgatgc agacaagggc tcggcgacgc cgtaggtaca
     1021 catttctgct ctcggcctga cattcatgca cgtcatacca tgcctgtatt tgcttacaga
     1081 gtggctcgtc ctgatggctc aaccctcgat ggacagattg agggagagga agaacagctc
     1141 gtccgggcca aactggaggg gcagggattt ctcgtatttc ggttacagcg acggggtgcg
     1201 gccaccaccg gcttaggtct ggattggcac gcatttggaa agctcccgct tcaggaattc
     1261 ctggtgttca atcaagagct gctggccctc gtgaaggccg gtcttcccgt gttgcgagtg
     1321 tgggatctct taatcgagcg agcgagccat tcagggtttc aacaggcttt gcggacggtc
     1381 cgacaagata ttcgtggagg ggcgtcggcc tcggaagccc tggccaaaca cccgatccat
     1441 ttttcagaat tgtatattgc gacaatcaaa gccggagaac aatcggggaa tttggcggag
     1501 gtgctgcagc ggttcattgc gtatttaaaa ctcatgatcg ggttgcgtca gaaggtttcg
     1561 aaagcgcttg cgtatccggc gttccttgtg atggtcggaa tttcggtcat cggatttctg
     1621 ttgacgtacg tcgtgccgac attcgtgtcg gtctatgcgg aatcgtcaaa gtccttaccg
     1681 gtcgccacgc aactgttgct cgatgtggtg gcgggaggca atgtgtatct gttgccagga
     1741 ttggtagggc ttgccgttct tgtgctcgta ggtcgcgcgt actatgtgac accggctggg
     1801 cgactcgttg tggatcagct cctcttacgt gtgcctgtct tggggccaat atttgtgaag
     1861 cattatacgg ttcagcttac gcggaccctt gcgacgatac ttgccggagg caccccgctt
     1921 gtcgatgcat tatcgattgc acggggcgcc atctccaatc gatatgtatc gttcggagtt
     1981 gccggtgcgg tcggtgaaat tcgcgaaggg acgaccctgg ccgccgcatt ggaccgcccg
     2041 aaagtatttc caaaattggc agtggaaatg ttgtcggtag gggaggagac cggctcgctg
     2101 cccgccatgt tgcacgatgt ggctgaattc tatgaagggg acctggatct ccggctcacc
     2161 caattgacaa cgtggattga accggcactg ctgctgatca tggggatttt agtgggtgcg
     2221 atcgtcatta tcatgtatct accggtgttt cagatggcgg gggccgtgta gcgctgaatg
     2281 cttcattgag cggttcggct tgcgctcttg ggtgaaggag cgagtaggaa actatggcac
     2341 gaacagtgac ccgaccgaca ttggcggatg tgctcataca gcagggggtt ctgccgaaac
     2401 agacgcttga ccaagtgctc ggcagactgg acggtgtcac tgccgctctc ggtcagactt
     2461 tagtaggcga agggattatc tctgaggatc agctggcccg tgcattggcg gcccagtttg
     2521 acttgccttg tgatcttctg acggagttcc gggttgacca gtcattctat aactccatgt
     2581 cggtcaaact catgcagcgg catccgttca taccgattgc agagcaccaa ggccgcctga
     2641 ctattgccat atctgatccg cagaatctcc tggcattgga tgagttagaa ctgctcttgg
     2701 ggcgcccgtt agacctcgtg gtgagctcgc gaagcgcgat tctggcggca ctggaacgta
     2761 gtgaaggctc cagtcaagcg cttcgggagc ttgaagccga gtatcgatct gtgttggtca
     2821 aggaggacga gcgtggagag gaagtcttca cgatcgacca cgagggagag gatcaaagcc
     2881 ctgcggtcaa actattggat tcgattctct taagcgcgat gcagcgccgg gccagcgaca
     2941 ttcacattga agccgccgat cgtgctacca aggtgaaatt gcgggtagat gggattctcg
     3001 ttccagcgat ggagccgctc gatatccgtc tccatgcgcc gttggtctcg aggttgaaag
     3061 ttatgtcgga tttggacatc gctgagcgac gagtaccgca agatggaagc ttccggatgc
     3121 ggctcgagcg aaagacggtc gacttccgag tctcaatcct cccgagcgtg ttcggtgaat
     3181 cggtcgtcat tcgtattcta gaccgtgagg cgatcaccac gggtgttgcg gcactacggt
     3241 tggaacgact cgggttcaat gtggaagatc tgaagcgttt tcggcgtgcg atcacgcgac
     3301 cctacggcat ggtgctggtg accggtccaa ccggcagcgg aaagacgacg acgctgtatg
     3361 cggcgattac ggagatgaac atcaaggagg acaagctgat cacaatcgaa gatccggtcg
     3421 agtatcaact gtcgggggtg gtacaaattc ccgtgaatga gaagaaaggc ctgaccttcg
     3481 cgcgagggct ccgatcgatc ttacgtcatg atcctgacaa gatcatggtc ggtgaaattc
     3541 gcgatgcaga gaccgctcaa atcgctatcc aatcgtcgct caccggccac ttggtcttga
     3601 cgacggtgca cgcaaacaac gtgttcgatg tgatcggacg atttgcctcg atgggaatcg
     3661 atgcctataa cttcttggct gcgctgaact gtgtgctggc gcagcggctt gttcgaatgg
     3721 tctgtcttgg ctgtcgagtg gcggtcaagc tcgaccgtgc gttggcggag gagtccggtc
     3781 tcgatttcga agaatataag gacgtcccat tttatgaagg caaaggatgc caggaatgcc
     3841 atggcaccgg gtatcgcgga cgaaaatgca tcacggagtt tctagatctg aatgatgaaa
     3901 tcaaagaaat gattcttgcc gaacgtcccc tgtcagaagt ccgctatcgc gcggtgacca
     3961 gtgggatgat cacgttgcgg cagtctgcgc tcagaaaggt gctggaaggc tccaccaccc
     4021 tgagggagat caatcgagtg acgttcagcg aggagggcta agggatgtgg gggctcagca
     4081 ccagccgtcc gcagcactgc ttgaaaatcg gcgcgcaagc actcgtctgg ggagaggccg
     4141 tccggacatg gcgggggcgg taccgctatc actgtgtcct ctctccgaca ccggccgggg
     4201 ccatcaagtt gtctccaatg gatttgaatc tgacagacat gtctacagtt gaaagtcgtc
     4261 ttcggtcgct ggctggacca agtcgagaca tccgcttcgc agggcgtgca tggctatccg
     4321 acgtccctcg ccccattgtg ttgttgctat cagatctgtc ggtgcgcgcg acagtgttgc
     4381 aactggaaca gttcccggca caggtagaag aacaagaggc gctgattcgc tggcgccttg
     4441 ggcaggagca gctgttgccc atgactgggg cgaagattgt ctggcagggg ttcccctcgc
     4501 gtggggcagg agatgagcgc tcccatgcga tttttgtcgt ggcaattcaa gaaaccatct
     4561 tgaagcaata cgaatccctc tgtgaatcgg tggggctact cccacaagag gtcggtgtga
     4621 ccagctttcg gctgtttaat ctgtggctga gagcggcagg cggtcggaaa cggttgggtc
     4681 atgacctgtt ctgggtgagt atttcggacg gcgggttgac ctgcttcgtg atgcagggag
     4741 gccgattggt atttgtccgg accaaactgt tgatcggcga aaggctacag ggtgaagaag
     4801 accttgagga agacctggtg aacaccattg ccgaggaatg cgcggtttct cttcgtacct
     4861 gtctcgaaca tcatcctggt ctgaacgtga cggagatcgt tcttgccagc gatagcccat
     4921 tcccgacgct tgagcaggcg ctgaaacgcg aacttggcct gtcgactgag agactggatt
     4981 gggaagctgt caaacggttg gggtggactc atgatggggg aagtacgtca tgggcgacgc
     5041 tccctgtcgt cgcgggggtg atgtagacga tggcgcctga acgaacatgg cttcgtgcgc
     5101 ttgccggcac gctaagtagc ttcatggtca taccaggagg ggcacggtat tttcacatcg
     5161 aattgagcag tcgataccgg tggtatctcg cacctgcccg tctgatgctg gccgcactca
     5221 gctgggtcat aggggcttca atatttttga gcgtggctca gaccgtgata accatgcgtg
     5281 acctgcgcga catgcaagca cgcatcgatc acgttcgcat gcaagatcgt cagctcgttg
     5341 ccgaggccca acaggaaggc attgatctct ccggtccgtc attgcaacag ctatcggccg
     5401 aagtcgggtt ggcgaaccag ttgcttacaa aacggaattt ttcctggaca cagtttttgt
     5461 ccgcgcttga agcagccatt cctcaacatg tgtcgattca gagcatacgc cttgatcccg
     5521 gtagcgcggt cgtacacatg atcgggctgg ctgcgacggt cgaagatgtg acggcattga
     5581 cggttacctt gcaagaccac accgcgttcc atgatccggt gctgggccag catcgggtgg
     5641 ggccgaatgg tttggttgag tttgacttga cgctgcggta tcggcagccg ggtgtataga
     5701 acgtcatgac ggatttcatc ttccaacagt tgcgaaaacc ctatgggatt ttgattccat
     5761 ggttcgcatt ggtgatgctc ctggccggag tcttgtgggc ggtccatacc gttgggtttg
     5821 atggagccga gcagaatcgg gccaaactgg aatcggaatg gaatcaagcc cgtcaagacc
     5881 tcaaacacca taaggaggca cagaaggcac gaaaggatct tgcacaggtg tggtcggtct
     5941 tgcctgttga acgggatttt gctcccttgg cactgggcat tacagaggag gcgaagcgtg
     6001 atcgtgtgat tctccctgcc ttgtcataca agactgagcc cacggctgta gccaacacga
     6061 gtaaggggtt gctgcagggc acgatgaccg gccgttacga ggatcttcga cgctttctct
     6121 atgatgtgga gacggcggaa gaactggtct acatcgagga tttggaactt gtgcaatcag
     6181 atgctcagga gaatcagaag ctcaccttca acatcaagat tgccacatac cttcgaggcg
     6241 attctggtaa ggcggcggtt cagtagcgcc taaatatgat gacgacgaat caaaagaaag
     6301 ctgcgacatt ggccacgctt ggcctactct ggggtgggat tgtggcttgg gagtggcaaa
     6361 ccatggaaga acccgtgcgt gtgccgctgg cgaatgtgag tggccctgtc tccacatccc
     6421 gggcagcccg tgggacaggc gaggggttgc acgtgcagct cgatctcctc gcagctgcgc
     6481 gaactcaacg cgagatgacg ttcacgccgc cacgcaatat ctttgcgccg cctcgcccta
     6541 atgacccgat gactacgcat gccaaccagt cggagttcga ccccgggttg gaccaaacat
     6601 tgcgccaaca ggaggccctt gcaggattga cacaattcca ctacctcggg tttgtccgca
     6661 tggaagaggg gtggaggaac aagggagaac tcgctgtgct cacgaagaac gacgatctcc
     6721 atgtcgtccg gaccggagag acggtggaag atcacgttgt ggtgaaggcg attacacaag
     6781 agagtgtgac gttgcaagat cgacaattgc gggtggagca tacggtgctg ctctccgagg
     6841 aagctccggt acagccttag caggatgctg aaaaagtccg ccagcttcgt tctcacctcg
     6901 aaagcatcct caacgtagcc cacagaggct acgcctccgg tgctttcatc ggctgcggcc
     6961 ttgctggacg gcctttttga gcatcctgcg cgatgttctc ccgtcgtact agatgtgtgg
     7021 accatcgaaa cttgagcgcg ccaaatagtt ttttcaccgt ctgtgatagg gttgctatgg
     7081 tcgtcagccg ccggtatcac tgcatgaatg agcgaggggt gacctacctc atgctcatgt
     7141 ttgcaattgt gctcattggt atcgccacga ccgctgcagc gaaacaatgg aaggccatcg
     7201 tccagcggga gcttgaagcg gatctcttgg ccaaggggat cgaaatccag aatgccctcg
     7261 ccttgtattc tgcgacgatc aaagcgggcc gtgtgatgcc gggggaggtc taccctcaga
     7321 cattggctga tctcacgaga ttacccaaac ccttcctacg gaaggtctat ctc
//
LOCUS       DATIPF010000251         1603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_136840, whole genome shotgun sequence.
ACCESSION   DATIPF010000251 DATIPF010000000
VERSION     DATIPF010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136840"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..116
                     /locus_tag="VLL06_06355"
     CDS             <1..116
                     /locus_tag="VLL06_06355"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HSS30622.1"
                     /translation="FTLTVAAAEVAVGLAIVIALYRSKSTINIDEFQLLKW"
     gene            220..>1603
                     /gene="nuoL"
                     /locus_tag="VLL06_06360"
     CDS             220..>1603
                     /gene="nuoL"
                     /locus_tag="VLL06_06360"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HSS30623.1"
                     /translation="MTYALIPLLPFAAFLILGIFGHWIKDKAHLVAVPAVVVSWLLAL
                     MVFFDVADGHQTSFPLYTWLTSGPLDIHIGFSIDRLTAVMLLLVTTVSSLVHIYTIGY
                     MHGDSGYARFFSYIALFTFSMLMLVMADNLLQLFVFWEAVGLCSYLLIGHWYERPAAC
                     SGATKAFIVNRVGDFGFMLGLLLVWYSFGSLDYSEIFVRAHESAGDVMNVLGPFGGTW
                     DVSVLTLICILLFTGAVGKSAQVPLHVWLPDAMEGPTPISALIHAATMVTAGVFMVAR
                     LAPLYNLSPTAMTLVAITGGVTMVVGATIALTQTDIKRVVAYSTVSQLGYMIMACGLG
                     AYAASMYHLLTHGAFKALLFLGCGSVIIALHHEQDMRRMGGLKHKLPITYWTFVVGSL
                     ALAGFPLTSGFFSKDHLLVSAWSSGPLGQFLTVLGLVTALMTAFYSFRLVFVTFWGPS
                     HVDPHHADHVH"
BASE COUNT          271 a          480 c          425 g          427 t
ORIGIN      
        1 tctttaccct caccgtcgca gcagcagaag tcgcggtcgg gttggccatt gtcattgcct
       61 tgtatcgatc aaaatccacg atcaacatcg acgaatttca attgctgaaa tggtaggacc
      121 ggatactatg ttgaatgatg aatgttcaaa gttcagtgag cggaatggat taggggcctt
      181 gtattcgaca gtacgcattg agcattgaac gttcctttga tgacctacgc actgattcca
      241 ctgcttccct ttgccgcctt cctgattctc gggatcttcg gacattggat caaggacaag
      301 gcccatcttg tcgccgtccc agcggtcgtt gtgtcgtggc tgctcgcgct catggtcttc
      361 ttcgatgtgg ccgacggcca tcagacgagt ttcccgctct acacctggct gacctcgggc
      421 ccgctcgata ttcacatcgg tttctccatc gaccgactca ctgccgtgat gttgctgctc
      481 gtcacgaccg tgagttcgct tgtgcatatc tatacgatcg ggtacatgca cggagattcc
      541 ggctatgccc gcttcttcag ctatattgcc ctcttcacct tttcgatgct gatgttggtc
      601 atggcggaca acctgcttca gctcttcgta ttctgggaag ccgtcggtct ctgttcctat
      661 ctactcatcg gccattggta tgagcgtccg gctgcctgct ctggcgccac gaaggccttc
      721 atcgtcaacc gcgtcggcga cttcggcttt atgctcggcc tcctgttggt ctggtacagc
      781 tttggctcac tcgactattc agaaatcttt gttcgtgcgc acgagtccgc aggcgacgtc
      841 atgaatgtcc tcggtccgtt tggaggcacc tgggacgtct ccgtgctcac actcatctgc
      901 atcttattat tcacgggcgc agtcggcaag tcggcccaag tgccgctcca cgtctggctg
      961 ccggatgcga tggaaggtcc cacccccatc tcggcactca ttcatgccgc cacgatggtc
     1021 acggcgggcg tgttcatggt tgcgcggttg gctccactct ataacctgtc tcctaccgcc
     1081 atgacgctcg tggcgattac cggtggagtg acgatggttg tgggggcgac catcgccttg
     1141 acccagaccg atatcaaacg tgtcgtcgcc tactcaacag ttagccagct cggctacatg
     1201 atcatggcct gcggtctggg cgcctatgcc gccagcatgt accatctact gacccacggt
     1261 gccttcaaag ccctcctgtt tctcggttgc gggtccgtca tcatcgccct gcatcacgaa
     1321 caggatatgc ggcgtatggg cgggttgaaa cacaaactgc cgattaccta ttggacgttc
     1381 gtggtgggtt cgctcgcgct cgcaggattc cccctaacgt cgggattctt cagcaaggat
     1441 catctgcttg tgtcagcgtg gtcgtccggc cccctcggtc agttcctgac cgtgctcggc
     1501 ctcgtgacgg cgttgatgac ggcgttctac agtttccggc tggtcttcgt cacgttttgg
     1561 ggtccttcgc acgtcgatcc tcatcatgca gaccatgtgc acg
//
LOCUS       DATIPF010000252         2876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_843342, whole genome shotgun sequence.
ACCESSION   DATIPF010000252 DATIPF010000000
VERSION     DATIPF010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843342"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            359..892
                     /locus_tag="VLL06_06365"
     CDS             359..892
                     /locus_tag="VLL06_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30624.1"
                     /translation="MKYVLQVVTVTCLVMAGVGAWVPPGYGEPYELSKNDVMDPKALK
                     GAEVSLFGVKLGDSEAKALDSLVNEKIPGVKAEQEGLFIFLLDQRKPTGPMAGVRIQD
                     GRVDLIFINNRFSYKTRGIFRNVLNSESPDDVRKLLGQEDYGDENVMGAILAYDKQGF
                     LINYLGKDVNVEFSPIR"
     gene            complement(1147..2010)
                     /locus_tag="VLL06_06370"
     CDS             complement(1147..2010)
                     /locus_tag="VLL06_06370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HSS30625.1"
                     /translation="MLIGLLLISAATVAPSIADAKSAATHPVQVRTAIVPHQFPPWRV
                     VPAHGILLKELNSGRVLYEHDAGKRMSPASLTKIMSALVILERGKLDDLVTISPNAAR
                     AHKMHLRVKAGQIFRLEDLLKAMLIMSANDACLAAVEHVGGDEAQFVTFMNEKAAALG
                     LEDTHFRNGCGFDNPDHYSTAEDLAALSVIALDQPIFRQLVREEHAIITPVSGHRAYI
                     LHNTNRLLGRVPGVEGIKTGFTSKAGRCLIAKVSQNGSDLLLVILNSKRRWNTATHLI
                     TYGLQAAEVSH"
     gene            complement(2313..2513)
                     /locus_tag="VLL06_06375"
     CDS             complement(2313..2513)
                     /locus_tag="VLL06_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30626.1"
                     /translation="MRSFRWWFSIGALLTLSVLMVQGGVRDLFDGAPPGSGASAYLSF
                     GTTIFGGGLLAGCLALVMNRLR"
     gene            complement(2645..>2876)
                     /locus_tag="VLL06_06380"
     CDS             complement(2645..>2876)
                     /locus_tag="VLL06_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30627.1"
                     /translation="QSSDLSILFGRDDGTFEYPPRNYRTKPGPFALATFRVTTKEAEE
                     PGLVTADNGNGSVSVFLHRGLKAAPAPVAIPP"
BASE COUNT          708 a          807 c          717 g          644 t
ORIGIN      
        1 ctacttccaa agacttaggc gcctcggaac ccttctcatt actacccatc acacgaccta
       61 acctatccct atggaaacga ccgggaacgg atcgttccat agacattgtc gcaaggggat
      121 tcggtatcct ccgtcgaaca accatttccg ccgcctcgcc cgcccaacgc acagtcaccg
      181 cggcactata accacgtaca agaccgaggg tcttgattcc aatcacctct tcagccgtgc
      241 cttgcaagtt ctcaggtcgt tcgataaaat gtctggacac cgctttggtc ccgtcaattt
      301 tgccaaataa acttcacaca ccatacatct cttggaaccc atgggtaagg aacaatccat
      361 gaagtatgta ttgcaagtcg tcaccgtcac ttgcctggtg atggccggag tcggtgcatg
      421 ggtgcctcct gggtatggcg agccctatga attgagcaag aacgatgtga tggaccccaa
      481 ggccctgaag ggtgccgagg tttcactgtt tggcgtcaag ttgggagact ccgaagccaa
      541 agccttggac tctcttgtga acgaaaagat tcctggagtg aaagcggaac aggaaggctt
      601 atttattttc ttactcgacc aacgcaaacc gaccggtcct atggctggcg tgcgcattca
      661 ggacggcagg gttgatctga tctttatcaa taatcgattt tcttataaga ctcgcggcat
      721 ctttcgcaac gtgttgaaca gcgaaagccc cgatgacgtg cgaaaactat tagggcaaga
      781 agattacggc gatgaaaatg taatgggcgc catactggct tacgataaac aggggtttct
      841 catcaattat ctggggaaag atgtcaacgt cgagttttcc ccgattcggt aatctcgtgg
      901 accggatgtt caacccccac tacacacctg cagaactaga actcttccac ccttctcctc
      961 agttgccggc aggaattcgt tcctgttccg tttctccgca tctgttccca caagtctccc
     1021 caagtcggta agtcctcgcc cgtgctcttg gagggattcg aacggtcaga ctgcagatag
     1081 ccatcaggct gcgatggcat tctcatattc agacgcgccc atttcgactt gagtaagcag
     1141 gaagcttcag tgcgaaactt cggctgcctg taatccgtag gtgatgaggt gcgtcgcggt
     1201 gttccagcga cgcttggaat tgaggatcac gaggagcaga tcgctcccat tttgagaaac
     1261 tttggcaatc aggcaccgcc cagccttgga cgtaaatccg gtcttgatcc cttcgacacc
     1321 ggggacacgt cccaggagtc tgttcgtatt gtgcaaaata tacgcacgat gaccactgac
     1381 aggcgtgatg atcgcatgct cctcacgaac caactgccgg aagatgggct gatcgagcgc
     1441 aatgacgctt aatgccgcga ggtcttcggc cgtagagtaa tggtccgggt tatcgaaccc
     1501 gcagccattc ctaaaatgtg tatcctccaa gccgagggcc gcagccttct cattcatgaa
     1561 cgtcacaaac tgtgcttcgt caccccccac atgctccaca gcggcaagac aagcatcatt
     1621 cgcggacatg atcaacatgg ccttcaacag atcttccagc ctaaagatct gccctgcctt
     1681 cacgcgtaga tgcatcttgt gagcccgtgc ggcgttggga ctaatcgtga cgagatcatc
     1741 caactttccc ctttccagaa tgaccaaggc cgacatgatc ttcgtaaggc tggcgggaga
     1801 cattcgcttc ccggcatcgt gttcgtataa cacacgacca ctattgagtt ccttaagtag
     1861 aatgccatgg gctggcacga ctcgccacgg agggaactgg tgcggaacaa tcgccgttct
     1921 gacctgtaca ggatgagttg ctgcgctctt tgcgtcggcg atggatggag cgacagtcgc
     1981 ggcactgatc aatagaaggc caataagcat ctccgcagcg cggatgagat ataacggtcg
     2041 acgggatctc gtgcagtctc ttgtcatcat gaaaccgtgg ctcctactca taatactcat
     2101 agggtatatg attcccgcac tttccgaccg cgaaacccgc tgtcaattac tttatgaaaa
     2161 aagcgaagta gatcggcaag gtcgatccag aacccgcaat tcaaacagcg ggcatagaga
     2221 cgccgattca ccagcgtata gtgacgctcg accatcatga aacccttgca tcttaaacaa
     2281 tgcattgacg cgcctgtctc cagactctct ctctatcgga gtcgattcat caccaatgcc
     2341 aagcacccgg ccaacaatcc tccgccaaaa atcgtggtgc caaatgaaag atacgcgctg
     2401 gctccactgc cgggaggggc cccatcaaac aggtcacgta cgcctccctg taccatcaac
     2461 acggagaggg tgagtagtgc gccaatagag aaccaccatc gaaatgatct cactggtatc
     2521 cccaacacag agcgccaatg ggcctgaaaa cagcgcccat cggccactgt agcggagggt
     2581 ccagagactg tcaagaagac tgactattga ctctctcaac ctcgggatag acgtcttaca
     2641 atgcttacgg aggaatcgcg acgggagcag gcgctgcttt caatcctcga tggagaaaga
     2701 cggacacgct tccattgccg ttgtccgccg tcacgagtcc gggctcttca gcctccttgg
     2761 ttgtcacgcg aaacgtggcg agggcgaagg ggcctggctt tgtccgatag tttctcggcg
     2821 gatattcgaa ggtcccatcg tcacgaccaa agagaataga gagatcactc gattga
//
LOCUS       DATIPF010000253         3696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_136958, whole genome shotgun sequence.
ACCESSION   DATIPF010000253 DATIPF010000000
VERSION     DATIPF010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136958"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..180)
                     /locus_tag="VLL06_06385"
     tRNA            complement(105..180)
                     /locus_tag="VLL06_06385"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Cys,seq:gca)
     gene            complement(265..1446)
                     /gene="ispG"
                     /locus_tag="VLL06_06390"
                     /gene_synonym="gcpE"
     CDS             complement(265..1446)
                     /gene="ispG"
                     /locus_tag="VLL06_06390"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HSS30628.1"
                     /translation="MHITRRKTRQIKIGKLKIGGDAPVSVQSMCSTDTRDAAATIEQI
                     RQLEAAGCEVIRVAVPDDEAAAVLPKIKAAMTVPLIADIHFDHRLALKAAEIVDCVRI
                     NPGNIGAWWKVEEVIKAVNDHGIPIRVGVNGGSLERDLLDKYGWPSPEALAESALNAV
                     HALEDVGFTNMKVSLKASDVHHAIDAYWLFAHQSDYPLHIGITEAGTAMTGAVKSTMG
                     LGYLLSQGIGDTLRVSLAADPVEEVKVGFEILKSLELRHRGINVIACPTCGRVEIDVV
                     KLANELEKKLGHIKTPLNVSVLGCVVNGIGEGKEADIGIAGGEGKGILFKKGKLVRKV
                     PIEELMDTLIHEVELMAKEKDAEENGEVAVHSNEGWESMGPQSSQPATLGKEIPVLPN
                     R"
     gene            complement(1496..3445)
                     /gene="dxs"
                     /locus_tag="VLL06_06395"
     CDS             complement(1496..3445)
                     /gene="dxs"
                     /locus_tag="VLL06_06395"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HSS30629.1"
                     /translation="MSILKTIQSPADLKRLPPTLYPALCQELREQIIGVVSNVGGHLA
                     SNLGVIELTVALHSLLNTPKDKIVWDTSNQAYAHKLLTGRREGFHTLRQYGGLSGFCK
                     REESEYDTFNAGHAGTGVSAAYGMVEAREQLKQRHKVVCVVGDGAMTAGMTLEGLQHA
                     GGLGKDFLVILNDNQMSISKNVGAISSYLNRTITGDFYGKMREETGQLLGKIPHIGQD
                     MQKWARRAEELAKGAILPGLLFEELGFRYAGPIDGHNFEHLLPTLENVLKMKGPVLLH
                     VITKKGLGYEPSVNNPVWFHACPPFVRETGAPAKKAARPSYTQLAIESLVKLARKDKR
                     IVAITAAMCEGTGLNIFEKEFPDRFYDVGIAEQHAVTFAAGLAAQGLRPVVALYATFL
                     QRAYDQVVHDVATQNLPVTFCIDRGGLVAEDGTTHHGAFDYAFLRHVPNMVVMAPKDE
                     NELQHMMKTCVQYDGPASVRYPRGLSLGVTMDQEQKAFPIGKGDLLCDGTEVAIMAIG
                     VPVSHAVAAAKRLEEEGISTAVVNARFVKPLDQDLIVEVAKRVRYVVTVEEGCKMGGF
                     GSAVLEALSDGGVTDVMTKVIGLPDWYIEQGPQDFLRERYGLTADGIYQSVKELIDRI
                     PATSREKFTFASSADRLPHGDEQGS"
BASE COUNT          778 a         1134 c          923 g          861 t
ORIGIN      
        1 tgaggggtag agaactgccg gtaccgggaa gagggatagc ctttgagggt tttatcccgt
       61 taccttgaat aatcatacgg gatccgagcc gagcaaactc tgtttggagg cgccgagcgg
      121 ggtcgaaccg ctgcatcgca gttttgcaga ctgctccctt aaccacttgg gtacggcgcc
      181 ccgggcacat tatagcgagc cttctttcca tatctcaatg gataggttgg aaggtgagga
      241 cgaagaaatt cggcaggagc gtgactatcg gttgggcaac acaggaattt cttttccgag
      301 agtggcaggc tgactcgact gcggacccat cgattcccat ccctcgttcg aatgaaccgc
      361 gacctcccca ttctcttcag cgtctttttc cttagccatc agctcaacct catgaatcaa
      421 cgtatccatc aactcctcaa tcggcacctt ccggacgagc ttgcctttct tgaacagaat
      481 gcccttgcct tcgccgccgg cgatcccgat atccgcttcc ttcccttcgc cgataccgtt
      541 cacgacgcaa ccgaggaccg acacattgag gggcgtctta atatggccta gcttcttttc
      601 cagttcattg gccaacttca ccacgtcgat ctctacgcgg ccgcaggtgg gacaggcgat
      661 gacattgatc cctcgatggc gaagttcgag cgacttcaag atttcgaacc ccaccttcac
      721 ttcctcgaca ggatcagcag cgagtgacac gcgcaacgtg tcgccgatcc cctgcgagag
      781 caaatacccg agacccatag tggatttgac ggctccggtc atcgccgttc cagcctctgt
      841 aataccgatg tgcagcggat agtccgactg gtgcgcaaac aaccagtagg catcaatcgc
      901 atggtgcaca tccgacgcct tcagcgacac cttcatgttc gtgaatccca cgtcttctaa
      961 tgcatggacg gcgttgagag ctgattcggc cagcgcttcc ggcgagggcc acccgtactt
     1021 gtccaacaga tcccgttcca gcgagcctcc attgacaccc acgcgaatcg gaatgccgtg
     1081 atcgttcacg gccttgatga cttcttcaac tttccaccag gctccgatat tgcccggatt
     1141 aatcctgacg cagtccacga tctcggcagc tttcaatgcc agccgatgat cgaaatgaat
     1201 atcggcgatc agcggcacgg tcatcgccgc tttgattttt ggtagcacag ctgccgcttc
     1261 gtcgtccggc acggccaccc gaatcacttc acaccccgca gcctccagct ggcgaatttg
     1321 ttcgatcgtc gcggcggcat cgcgtgtgtc tgtcgaacac atcgactgga cggacacggg
     1381 cgcgtctccg ccgattttga gcttgccgat tttaatctgc cgcgttttcc gtctcgtgat
     1441 atgcatggag tctcttcgct accttgatct caggtctagc gtttcatgtc ttcgatcagc
     1501 tcccctgttc atctccgtgt ggaagccgat cggccgatga ggcaaaggtg aacttctctc
     1561 gtgacgtagc cggtatcctg tcgatgagtt ctttcacgct ctgatagatc ccgtccgccg
     1621 tcaacccata tcgctcgcgc aggaagtcct gcggtccctg ctcaatgtac caatccggca
     1681 acccaatgac ctttgtcatg acatccgtca cgccgccatc ggacagagcc tcgagaacgg
     1741 ctgagccgaa cccacccatc ttgcaacctt cttcaaccgt caccacgtag cgaacccgct
     1801 tcgccacctc gacgatcaag tcctgatcca gaggcttcac aaaacgcgca ttgaccaccg
     1861 ccgtggaaat cccctcttcc tcaagacgtt ttgccgctgc cacggcatgc gagaccggca
     1921 caccgatggc catgatcgcc acctccgtac catcgcacaa taggtcgccc ttcccgatcg
     1981 ggaacgcctt ctgctcctga tccatggtga cgccaagact cagtcctcgc gggtaccgca
     2041 ccgaggcagg cccgtcatat tggacgcagg ttttcatcat atgctgcaac tcattttcgt
     2101 ccttcggcgc catgacgacc atgttcggaa cgtggcgcaa aaacgcataa tcgaatgccc
     2161 cgtggtgcgt cgtcccatct tctgctacca gcccgccgcg atcgatgcag aaggtcaccg
     2221 gtagattttg cgtggcgaca tcgtgtacca cctggtcata cgcacgctgt aagaatgtgg
     2281 cgtacaaggc cactacggga cggaggccct gcgccgcaag acccgccgca aatgtcaccg
     2341 catgttgttc ggcaattccc acgtcataga accggtccgg aaactctttc tcaaagatat
     2401 tgaggccggt cccttcgcac atcgcagccg taatcgcgac gatccgcttg tccttgcggg
     2461 ccagtttgac caatgattcg atggcgagct gcgtatagga cgggcgtgcc gcttttttcg
     2521 ccggtgctcc ggtttcccga acaaaaggag gacaggcatg gaaccagacc ggattattca
     2581 ccgatggctc ataacccagg ccctttttcg taatgacatg gagtaacacc ggccctttca
     2641 tcttcaacac attttccaat gtcgggagca gatgctcgaa attatgtccg tcaatcggcc
     2701 ccgcatacct gaatccaagc tcttcaaaga gcaacccggg aaggatcgct cccttggcca
     2761 actcttcagc ccttcgagcc cacttttgca tgtcctgacc gatgtgaggg attttcccta
     2821 acagttgccc tgtttcctct cgcatcttgc cataaaaatc tccggtaatg gtccggttca
     2881 aataggagga aatggctccg acgttcttcg agatggacat ttgattgtcg ttcagaatga
     2941 cgaggaagtc tttccccagc cctccggcat gctgcagacc ttccagcgtc atcccggccg
     3001 tcatcgcgcc gtcgcctacg acgcagacga ccttgtgtcg ttgtttcaac tgttcacgcg
     3061 cctcgaccat gccgtaggcc gcggaaacgc cggtccccgc atggcctgca ttgaacgtat
     3121 cgtattcact ttcctcacgc ttgcagaagc cgctgagccc tccatactgc cggagcgtat
     3181 ggaacccctc gcgacgcccg gtcaataact tatgcgcata ggcttgatta ctcgtgtccc
     3241 acacgatctt gtccttggga gtgttgagga gagaatgtaa agccaccgtg agttcaatca
     3301 caccgaggtt cgaggccaag tgccctccga cgttggatac cacgccgata atctgttccc
     3361 gcagttcctg gcataaggcc ggatacagcg tcggtggtag ccgtttcaga tcagctgggc
     3421 tttgaatggt cttcaaaatc gacatggaga tgctcctttg cgtcaatgcg tacgcgcgta
     3481 cgctggtaca acgagttaag ccctgtgacc actggagaga aagtgtggca gcttggcgct
     3541 cgcaggggtg agtttcacat gggaatccca tattgtcaag gggttatgcc aatttcatta
     3601 tcgcatttcc cgcagcactc atgatgtata tcgataggtc aacaatgtcg agagtgtgaa
     3661 atccgctgcg ctcccggtaa tgaagtcaag attctc
//
LOCUS       DATIPF010000254         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1280998, whole genome shotgun sequence.
ACCESSION   DATIPF010000254 DATIPF010000000
VERSION     DATIPF010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280998"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /gene="uppS"
                     /locus_tag="VLL06_06400"
     CDS             <1..624
                     /gene="uppS"
                     /locus_tag="VLL06_06400"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HSS30630.1"
                     /translation="GIKSVREMITGCLELGIHALTIYAFSQENWNRPIQEISSLMGLL
                     EYYLSTERAKLIEQGVRLRTIGRLDTLPASALQWVRAAEQETAHLDRLHLTVALSYGG
                     RSEIVDAVRELLHEAQDGKLQPDDVDESRIQQYLYTHELPDPDLLIRTSGETRISNFL
                     LWQLAYTELYFTPTLWPDFRRRELLLALLEYQRRERRFGRVLSTISS"
     gene            645..1520
                     /locus_tag="VLL06_06405"
     CDS             645..1520
                     /locus_tag="VLL06_06405"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HSS30631.1"
                     /translation="MQEYKTTGQQQAEPTPVTAPRFDPRRIYTILVLAPLVYAVIRYL
                     PPLAFSGVVVLAGGLALFEFYRICFSGRNQFWLMGIGLTGFAALIFETHQIDLIGPTL
                     LVTLVCIISVPLFSHSPLEESLKDGAMTLFGVLYLGLTLGMLSMTRLLPQGEWLIFFL
                     LLVTWASDTGAYYIGTLYGRHRLAPRISPKKTVEGLVGGLIGAIIVAYVARWWFLPEL
                     SGLDCLVLAILLTIAGLWGDLTESAMKRSVGMKDSGGILPGHGGMLDRLDSLLFAAPA
                     FYYYVTLASRLRVTE"
     gene            1531..>2484
                     /locus_tag="VLL06_06410"
     CDS             1531..>2484
                     /locus_tag="VLL06_06410"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HSS30632.1"
                     /translation="MKTIVILGSTGSIGTSTLDIVDRFPEEFRVAGLTAGSNDEKLEE
                     QIRRYRPRVVALSDASAAARLRPRCAGLSVEILAGEEGLIQVASAPDAELVISAIVGG
                     AGLVPTLAAIRSGKQIALANKEPMVMAGKLMQDEAQRHGVRIFPVDSEHSAIFQSLEG
                     HRKEDVRRLILTASGGALWTLGREELQEVTPERALQHPNWKMGAKITIDSATLMNKGL
                     EVVEARWLFDIPETQIEVMIHRESIIHSLVEYEDRSMIAQLGLPDMRTPISYAMRYPG
                     RLPLDLPSLDLTEIAKLTFCKPDHDRFPCLGLGYESLRIGGT"
BASE COUNT          521 a          736 c          659 g          568 t
ORIGIN      
        1 gggatcaagt ccgtccggga gatgatcacc ggctgcttgg aactcggcat ccacgcactc
       61 acaatttacg cattttcaca ggagaattgg aaccgtccca ttcaggaaat ttcctcgctc
      121 atgggcctgc tcgaatacta cctctcaacg gaacgagcga aactcattga gcaaggtgtt
      181 cgattacgca caatcggccg actcgacact ctcccggcct ctgcccttca gtgggttcgg
      241 gccgccgagc aagaaaccgc ccatctcgac agacttcatc tcacagtcgc cctcagctat
      301 ggcggccgct cagaaatcgt agacgccgtg cgtgaactcc tgcatgaggc acaagacgga
      361 aagctgcaac cggacgatgt ggatgagtcg cgtattcagc aatatctcta tacccatgag
      421 ctgcccgatc ccgacctgct gatccgcacc agtggcgaaa cgcgaatcag taactttctc
      481 ctctggcagc tcgcctacac cgagctctac ttcactccaa ccctgtggcc cgacttccgt
      541 cgccgcgaac tcttattagc cttgctggaa taccagcgac gcgagcggcg attcggtcgc
      601 gtcctcagta ccatttcttc atagtggttg acctcaatct ccacatgcag gaatacaaaa
      661 ccaccggcca acaacaggct gaaccaacgc cggttacggc accccggttt gatccccgcc
      721 ggatctatac cattctggtt ctcgctccac tcgtatatgc cgtgattcgc tatctccccc
      781 ccctcgcatt ttcaggtgtc gtcgtgctgg cggggggact tgccctcttc gaattctacc
      841 gaatatgttt cagtggccgg aaccagttct ggctgatggg gatcggcctg acgggattcg
      901 ccgccctgat tttcgaaacg caccagattg acctcatcgg tccaacactc ctcgtcacac
      961 tggtgtgtat catttcagtc ccactcttca gtcactcacc cctcgaagag agcctgaaag
     1021 atggggccat gaccttgttt ggcgtgctct acttgggcct cacactcggc atgctttcga
     1081 tgacgaggct cctgcctcag ggtgaatggc tcattttctt cctcttactg gtgacctggg
     1141 cctccgatac cggcgcctac tatattggca cactgtacgg acgacatcgc ttggctccca
     1201 ggatcagtcc aaaaaaaacc gtcgagggct tggtcggtgg cttaattggt gccataatcg
     1261 tcgcgtatgt cgctcgttgg tggtttctcc ctgaattatc cggtcttgac tgcctggtcc
     1321 tggctatttt attgaccatt gcaggtctct ggggtgacct gaccgaatcg gccatgaaac
     1381 ggagcgtcgg catgaaggat tccggcggga tactgccggg gcatggtggt atgctcgacc
     1441 gtttggatag tctcttgttc gccgcccctg ccttctacta ctatgtaacg ttggcaagcc
     1501 ggctacgagt cactgagtga aggagcccct atgaaaacga tcgtcatcct cgggtcgact
     1561 gggtcgatcg gcaccagcac cttagatatc gtagaccggt tccccgaaga attccgcgta
     1621 gcaggcctga ccgccggcag taacgacgaa aaactcgaag agcaaatccg ccggtatagg
     1681 cctcgcgtgg tggccctatc ggatgcctcc gctgcagcca ggctccggcc gcgctgcgca
     1741 ggtctgtccg tcgaaatttt ggctggggaa gagggcttga ttcaggtcgc ttctgcaccc
     1801 gacgcagaac ttgtcatctc cgccatcgtc ggtggagccg gtctggtgcc tacgcttgcg
     1861 gcgatccgaa gcggaaagca aatcgccctg gccaacaaag agccgatggt catggcaggg
     1921 aaactaatgc aggacgaagc acaacggcat ggtgtccgta tcttcccggt cgacagcgaa
     1981 cacagtgcca tcttccagtc gttagaaggc catcgcaaag aagatgtccg ccgtctgatc
     2041 ttgactgcat ccggaggggc gctctggacc ttgggacggg aggaactgca agaggtcaca
     2101 cccgaacggg cactccaaca tcccaactgg aaaatgggcg ccaagatcac gatcgattct
     2161 gctaccctta tgaacaaagg tcttgaggtg gttgaagccc ggtggctctt cgatatcccg
     2221 gaaacccaga tcgaggtcat gatccatcgg gaaagtatca ttcactctct ggtagaatat
     2281 gaagatcgtt caatgattgc gcagttggga ttgccggata tgcgaacacc tatttcttat
     2341 gccatgcgat atccgggccg cctgccgctg gaccttcctt cgttggatct gacggagatc
     2401 gcgaagttga cgttctgcaa accggatcac gaccggttcc cctgcttggg tcttggctat
     2461 gaatctctgc ggatcggcgg tacg
//
LOCUS       DATIPF010000255         3057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_580342, whole genome shotgun sequence.
ACCESSION   DATIPF010000255 DATIPF010000000
VERSION     DATIPF010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580342"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /gene="argJ"
                     /locus_tag="VLL06_06415"
     CDS             complement(<1..1001)
                     /gene="argJ"
                     /locus_tag="VLL06_06415"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HSS30633.1"
                     /translation="MTENTPTASPRRGITAPQGFRAAGIHCGIKKPGLLDLALVASEQ
                     SGPIAGVFTRNQVIAAPVIVDRLHLRKGIGRAILINSGNANACTGAKGLAAAKLTATS
                     IARRMNIPSHQVFIGSTGVIGRVLPVNRIIKGIPQLLSQLSDHGGLDAAQAIMTTDLR
                     PKSVALQDTIGGRLITIGGMAKGSGMIHPNMATMLAYFTTDASITKSALQQALRLAVN
                     ESFNCISVDGDTSTNDTVLCLANGFAKNRTIKRGTVAFRRFLLLLTEACQALALAICR
                     DGEGVTKVVNIEVTGGTTVAQARQVAETIATSNLVKTALFGEDANWGRVMAAIGRAGV
                     "
     gene            complement(1038..2111)
                     /gene="argC"
                     /locus_tag="VLL06_06420"
     CDS             complement(1038..2111)
                     /gene="argC"
                     /locus_tag="VLL06_06420"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HSS30634.1"
                     /translation="MKKVRIAIAGASGYAGAELVRLAALHPYFELQTVTSEKSAGTPV
                     AAVFPSLAGLVSLSFQALSPEALADKADAIFLALPHTKSLAPVSACLQTGKLVVDLSA
                     DYRLKDPAIYEQWYHTPHTHAGLLKDAVYGLPELHRAAIARAKLVASPGCYPTAAVLQ
                     LAPLFTQDLVQPHTVVIDAKSGISGAGRSPALPFHFPEAHESLEPYNIGQHRHTPEIE
                     QELGGLLQRLSQSSGTIAIQPIVTFTPHLVPMNRGILSTAYCRLKSPMTLSDLRMLYR
                     TYYKDERFVRVQDNIMPNPRYIKGTNYCDIGIYMDPRSESVITVAALDNLVKGAAGQA
                     IQAMNLMLGLPEETGLTAPAAYP"
     gene            complement(2193..2585)
                     /gene="rpsI"
                     /locus_tag="VLL06_06425"
     CDS             complement(2193..2585)
                     /gene="rpsI"
                     /locus_tag="VLL06_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HSS30635.1"
                     /translation="MAVATHYATGRRKCAIARAWVTGRAGDITINDQPIEKAFPRLTL
                     RQIIQFPLEIGGVVGQYSIKATVHGGGPTGQAGALRHAIARALVELNQPLRTPLKKEG
                     LLTRDSRVKERKKYGQKGARKRFQYSKR"
     gene            complement(2620..3048)
                     /gene="rplM"
                     /locus_tag="VLL06_06430"
     CDS             complement(2620..3048)
                     /gene="rplM"
                     /locus_tag="VLL06_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HSS30636.1"
                     /translation="MLTYMQKPLDVQEKWYLVNAEGKTLGRLAARVAGILRGKHRPTF
                     TPHVDMGDHIVIINAEKIHLTGNKMATKLYRRHSGFPGGLKTATAQHVFRKDPTVLIT
                     KAIEGMLPKNPLGNHMAKKLRVYAGPTHPHQAQGPEPITL"
BASE COUNT          601 a          817 c          907 g          732 t
ORIGIN      
        1 acccccgccc gtccaatcgc tgccatcaca cgaccccaat tggcatcctc gccgaacagg
       61 gcagttttca ctaagttcga ggttgcgatg gtttcggcca cctgccgtgc ctgcgccacg
      121 gtggttcctc ccgtcacctc aatattcaca accttggtta cgccttcccc atcgcgacaa
      181 atggccaacg cgagtgcctg acaggcttct gtcaggagca gcaggaaccg acggaaggcc
      241 accgtaccac gcttgatcgt ccggttcttc gcaaagccat tcgccaaaca caggacggta
      301 tcgttggtac tcgtgtctcc atcgactgaa atgcaattaa acgattcatt gaccgctaat
      361 ctgagagcct gttgtagggc gctcttcgtg atcgaggcat ctgtggtgaa ataggccagc
      421 atcgtggcca tgttgggatg gatcattcct gagcctttgg ccatccctcc aatcgtgatc
      481 aagcggccac cgatggtgtc ctgcagtgcg acggatttcg ggcgcaggtc ggtcgtcatg
      541 atcgcctgag cagcatccag gcctccgtgg tcgctgagct gagagagcag ctgtggtatt
      601 cccttgatga ttcgattgac cggcaacaca cgaccgatta cgccggtgga tccaatgaac
      661 acttggtgtg acgggatgtt catgcgtcgc gcaatcgatg ttgcggttaa ctttgcggca
      721 gccaatcctt tggcgccggt gcaggcgttt gcgttaccac tgttgatgag gatggcccgt
      781 ccgattccct tgcgcagatg caggcgatcg acgataaccg gtgctgcgat gacctgattg
      841 cgggtaaaga cgccagcgat aggaccactc tgttccgagg cgacgagcgc aaggtcgagc
      901 aggcccggct ttttgatccc gcaatggata ccggctgcgc ggaacccttg cggtgcggta
      961 atcccgcgtc gtggtgatgc ggttggtgtg ttctcggtca taatcgtgaa cgaggcgtca
     1021 tgctcaagca ctgtggatta gggataggca gcgggagcgg tcaagcctgt ctcttcgggg
     1081 aggcccagca tgaggttcat ggcctgaatg gcctgacctg cggcgccttt cacaagattg
     1141 tccagcgcgg ctaccgtaat gactgattct gatcgagggt ccatgtaaat tccgatgtcg
     1201 caatagttgg tgcccttgat atagcgagga tttggcataa tgttgtcctg gactctgacg
     1261 aagcgctcat ctttgtagta ggttcgatag agcatgcgta ggtctgacag cgtcattgga
     1321 cttttcaagc ggcagtaggc ggtactcaag atgccgcgat tcatgggtac gaggtgtggg
     1381 gtgaacgtca cgatcggctg tatggcaatc gtcccggatg attgagatag gcgttgaagt
     1441 aacccgccga gttcttgttc gatctcaggg gtgtggcggt gctgcccgat attgtaaggc
     1501 tctaaggact catgggcttc aggaaaatga aaggggagtg ctgggcttcg tcccgcacca
     1561 gaaatgccgg atttcgcatc gatgactacg gtgtgaggtt gcacgagatc ctgcgtgaac
     1621 agcggggcca actgtagaac agccgcagtc gggtaacagc cgggcgatgc caccaacttt
     1681 gctctggcaa tggcagctcg gtgcaattcg gggagtccgt aaactgcgtc cttgagtaag
     1741 cctgcatggg tgtgcggtgt gtgataccac tgttcataga tcgccgggtc cttgagtcga
     1801 taatcagcac tgaggtccac caccagctta ccggtttgga ggcaggcgga gacaggcgcc
     1861 aatgatttgg tatggggaag ggccaagaag attgcgtctg ccttatccgc taaggcttct
     1921 ggtgaaagtg cttgaaacga gagagacacg aggccggcca gacttgggaa gaccgctgcg
     1981 acgggagtcc ctgctgactt ctccgatgtc acagtctgaa gctcaaaata gggatggaga
     2041 gctgccaggc ggacgagctc cgctccggcg tatccactgg cccctgcaat ggcgatgcgt
     2101 acttttttca tctgtatcac cagatctgta cgcacagaca taaaaaaggg aaggcttgga
     2161 gccttccctt tttctgactg tggctccgat gtctatcgct ttgagtactg gaaccgcttt
     2221 ctcgcgccct tctgtccgta ttttttccgc tctttgacgc gcgagtcgcg tgtcagcaag
     2281 ccttccttct tcaagggggt acggaggggt tggttgagct ccaccaacgc gcgagcgatg
     2341 gcatgacgta aggcaccggc ttggccggtc ggtccaccac cgtggaccgt ggccttgatc
     2401 gagtactgcc cgacgacacc gccgatttcg agcgggaatt gaataatttg ccgcagggtc
     2461 agccgtggga aggctttctc gatcggctga tcgttgatcg taatgtcgcc cgccctcccg
     2521 gtgacccagg ctctggcaat tgcacacttt cttcgtccgg ttgcatagtg agtcgcgact
     2581 gccatgctac aacgtctcct tcttcgttca cggtgatgat tacagggtaa tcggttccgg
     2641 tccttgcgcc tggtggggat gcgtgggccc ggcgtagacg cggagtttct ttgccatatg
     2701 atttcccagt gggttcttgg gaagcatacc ttcgatcgct ttggttatga gcacggtcgg
     2761 atcctttcgg aagacatgct gcgccgtcgc ggtcttgagc ccacccggaa atccgctatg
     2821 gcggcgatag agtttcgtgg ccattttatt gccggtcaga tgaatcttct ccgcattgat
     2881 aatcacaata tggtccccca tgtccacatg gggggtaaag gttggccggt gtttgccacg
     2941 caagatgcca gcgactcggg ctgccaatcg gcccaacgtt ttcccctccg cattgacaag
     3001 gtaccacttt tcctgcacgt ccaaaggctt ttgcatgtag gtcaacatcg ttctctc
//
LOCUS       DATIPF010000256         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_403361, whole genome shotgun sequence.
ACCESSION   DATIPF010000256 DATIPF010000000
VERSION     DATIPF010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403361"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VLL06_06435"
     CDS             <1..317
                     /locus_tag="VLL06_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30637.1"
                     /translation="MLGCTRGPTAVLDPVSRDPGQDHWAIASYYARQATASRHQAEEM
                     TNQAVVYERLFGRESDWVSGTRLLVQFYEEAAREQDRLADLHLELARVRSSNQPTQSR
                     GH"
     gene            355..1224
                     /locus_tag="VLL06_06440"
     CDS             355..1224
                     /locus_tag="VLL06_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HSS30638.1"
                     /translation="MKRLTLTTIALAVLLLTAACGTTIQPGQRGLFWHPFTEGLSTQP
                     LKDGFYWRAPWNNVYRYDIQWHSFTEDIDALSADDLQVLIKAAIIIRPIPDELYFLAQ
                     EVGQDYYPRIVKPQFMAAVRSVVSGYNMVKVPEKSAEIASKVRAVVVENLKGRHLELQ
                     NVALSDVDFPKIVLRAIEQKQAKEQEKEQKEFELTIASKDAQIARTRAKGEGDAIRIR
                     AEGEAEGLRIRAIGQAQAQTTISKTLTPAYLRYKLYDSPNSRMVLLPENLKAPIIINS
                     GESPGAEQRELTP"
     gene            1340..>2443
                     /locus_tag="VLL06_06445"
     CDS             1340..>2443
                     /locus_tag="VLL06_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30639.1"
                     /translation="MLFLPLSGCGAFLPYMYDAGKLDDRLAVSTPKEQVLKSFGKPDR
                     VVQDDGQQAIWEYRLYPKGEWVAYLLHCPFFPNCYFPAEPAQPYYLVLQDNQLCMWGT
                     PDVVRTLAWKVCAKAARPEGSQPIQALVRRGLQISVIPVFMPPTIFPLPQRLAVLPVA
                     GSTDARVISWLDFALNFLRTRHRELVLVEREDLRTVLQEVGIQYTGRVDEATTIRVGK
                     LVGADSLLTYRLAISGTGEPMSASFEIRLLTVESGITVFRQIASSTINLTMAEAATGR
                     PYELSPSARRLVIEEAAAYGLAALTAAFGDNPLGMVADYNWPGEGIKLLGLLQGGPAF
                     LAGLKPGDQILALNGQPLGSWIDPISLPIHVTVE"
BASE COUNT          536 a          667 c          709 g          531 t
ORIGIN      
        1 tcatgttggg ctgtacccga ggccccacgg ctgtactgga tcctgtctcg cgtgatcccg
       61 gccaagacca ttgggccatt gcgtcctatt acgcccgcca ggcgacggcg tcacgccatc
      121 aggccgagga gatgaccaat caggcggtgg tctacgaacg gctctttggg cgagagtctg
      181 actgggtgtc cggcaccagg cttttagtgc aattctacga agaagccgca cgcgaacagg
      241 accggcttgc tgatctgcac ctggaacttg ctagggttcg gtcgtctaat cagcccacac
      301 aatcgcgagg tcactaaccc gtcacgtttg gactggctat ttcgggaggt cactatgaaa
      361 cgactcacgc tgacgaccat tgcactcgcc gtactgctgc tgaccgcggc ctgcggcacc
      421 actatccaac cgggccaacg ggggttgttc tggcatccct tcacagaggg actttccacc
      481 caaccgctga aggacggatt ttactggcgg gcaccctgga ataatgtgta tcgctacgat
      541 attcagtggc atagttttac cgaagacatt gatgccttga gtgcggacga tctccaggtg
      601 ctcatcaagg cggccatcat tatccggccc attccggatg agctgtattt cctcgcccaa
      661 gaagtgggcc aggactacta ccctcggatc gtcaaacctc agttcatggc ggcggtgcgc
      721 agtgtggtct ccggctataa tatggtgaag gtgccggaaa agagcgcgga gattgcgagc
      781 aaagtccggg ctgtcgtggt tgagaatctc aaaggccgtc acttggaact tcaaaatgtc
      841 gccttatctg atgtcgattt ccccaaaatt gtcttgcgtg cgattgagca gaaacaggcg
      901 aaggaacagg aaaaagaaca aaaagaattc gaactgacca tcgcctccaa ggatgctcag
      961 atcgcccgca cgcgggccaa aggcgagggg gacgcaatcc ggattcgggc cgagggtgag
     1021 gcagaggggc tccggattcg ggccatcggg caggcccagg cgcagaccac catctcgaag
     1081 acactcacgc cggcctacct tcggtacaaa ttgtatgaca gtccgaattc taggatggtg
     1141 ctcttgccgg agaacctgaa ggcacccatt atcattaact ccggagagtc acccggcgca
     1201 gagcaaagag agctcactcc ctaagggcag ggcaggggag caaggattca gagacatgga
     1261 ccaatcacgt tcttccgcat gccaggggag agggcagacg aggagaaaac catattggtg
     1321 ttgtagactg ctctctctca tgttgttcct tcccctgtcc ggctgcggtg cgtttctccc
     1381 ctatatgtac gatgcaggaa aacttgacga ccgtttggcc gtgtcgactc cgaaggaaca
     1441 ggtgttgaag agtttcggga aaccggaccg tgtggttcag gatgatgggc agcaagccat
     1501 ttgggagtac cggctctatc caaaaggcga gtgggttgcc tatctgttgc actgtccctt
     1561 ctttccgaac tgttacttcc cagcggagcc tgcccaaccg tattacctgg ttctacagga
     1621 caatcagctg tgcatgtggg gcactcctga tgtggtccga acattggctt ggaaggtctg
     1681 tgcgaaggct gcgagaccgg agggatctca gccgatacag gctctcgtga gaagaggact
     1741 acagatatct gtgattcctg tgttcatgcc tcccaccatt ttcccactac cacagcggct
     1801 ggcggtgttg ccggtggctg gatcaacaga tgcgcgggtc atctcatggc tcgatttcgc
     1861 cctaaatttt ttgcgcacac gacatcgaga gttggtactt gtcgaacggg aggatctgcg
     1921 aacggtgctt caagaggtgg gcattcaata taccgggcga gtggatgaag ctacgacgat
     1981 tcgcgtgggt aaactcgtcg gtgcggatag tctgctgacc tatcgtctgg ccatatcggg
     2041 aacaggtgag ccgatgtccg cgtccttcga aattcggctt cttacagtgg aaagcggtat
     2101 cacggtcttt cgccagatcg cctcgtcgac aatcaatctc actatggcag aagcagccac
     2161 tggccggccc tatgagttat cccccagtgc ccgtcgtctt gtgatagaag aagccgcggc
     2221 ctatgggctc gcagccctca cggcggcatt cggcgacaat cctctgggaa tggtcgcgga
     2281 ctacaactgg cccggtgagg gaatcaagct gctcggcctc ctccaaggag gcccggcctt
     2341 cctggcagga ctcaaaccag gcgaccagat tctcgcattg aatggtcagc cgttaggaag
     2401 ttggatagat ccgatctcac tcccgattca tgtgaccgtt gag
//
LOCUS       DATIPF010000257         3894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_755306, whole genome shotgun sequence.
ACCESSION   DATIPF010000257 DATIPF010000000
VERSION     DATIPF010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755306"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VLL06_06450"
     CDS             complement(<1..536)
                     /locus_tag="VLL06_06450"
                     /inference="COORDINATES: protein motif:HMM:NF014923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HSS30640.1"
                     /translation="MRCAIVHYHELALKGRNREYFEERLVHNIQWTLKDLGIRRVENL
                     RSRIRVVLPEAVPDQTIITRLSRVFGIANFSLAHGLPLDLAHPNLGALSKAIIEALRS
                     ESFSTFRVSAKRADKRLTLTSMGVAREVGAAICDETGKKVSLKDPDLTVYLELLSKEV
                     YYATNKIQGPGGMPVGVSG"
     gene            complement(611..1537)
                     /gene="trxB"
                     /locus_tag="VLL06_06455"
     CDS             complement(611..1537)
                     /gene="trxB"
                     /locus_tag="VLL06_06455"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HSS30641.1"
                     /translation="MRNIVIIGSGPAGLTAAVYAARANLSPLLIEGWQSGGQLTTTTE
                     VENYPGFAKGIMGPELMKEMRAQAERFGTEFLTGDVSAVDLKTGPFSLTIDCEHTVHT
                     KTIIIATGASAIQIGLKNEVRLTGHGVSTCATCDGFFFKGKELIVVGGGDSAMEEATF
                     LTKFAAKVSIVHRRDKLRASKIMQDRAMKNEKIAFVWNSAVEDILGNDGVTGVRVKNL
                     VTGKVTEMPCAGVFVAIGHRPNTTLFAGQITMDEKGYIRTEKGTATSVPGVFAAGDVQ
                     DSVYRQAVTAAGSGCMAAIDAERFLESGSHNL"
     gene            complement(1583..2185)
                     /gene="coaE"
                     /locus_tag="VLL06_06460"
     CDS             complement(1583..2185)
                     /gene="coaE"
                     /locus_tag="VLL06_06460"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247713.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HSS30642.1"
                     /translation="MIVVGLTGGVATGKSTVAKMFKQCGAVVIDADELARDVVKPGKP
                     AWHAIVNTFGKTVLTPDRTINRQALGAIVFSNRVKLRRLERIIHPRVARQQAKLIRHA
                     VRNDPKAVVIYDVPLLFEVGIDKRVDKIIVVIADQETQLARLKKRNGLSRAEALRRIR
                     SQLPLAKKIQQTDHVLDGTLSRTSLRRQVGRLLKSLRLLA"
     gene            2273..3313
                     /locus_tag="VLL06_06465"
     CDS             2273..3313
                     /locus_tag="VLL06_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSS30643.1"
                     /translation="MKHVALLGLITSLVFWSFGPGGADEGPRLGPRTLVVALDGTGDY
                     ISLQEAVDAAMKGDTVFVKAGRYPQDVTIHSKEKIKFVGAGVDQVTILGRDIVVGALH
                     VGKWPYGATDIEISDMSINDRGGHAVGLFNGQGIVLRRVRINGMLFSQQVHDVRIEDS
                     VIGGSETTGAQFADSEAVLIGNFIHDNDHGVSIAGKSIVRLERNVITRSLFEAVVVSD
                     RAQAVLVSNTLVKNGGGAAFLGQSQSDVTGNVVGLNRVGFVIAPSSQISTAFNAFYNA
                     DGDYLRVGNPNQPAPELRAQSDITGDPHFVDPGHDDFRLRLDTPLLNIGCFSYLGALE
                     PASPSTPRSTKK"
     gene            3455..3640
                     /locus_tag="VLL06_06470"
     CDS             3455..3640
                     /locus_tag="VLL06_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30644.1"
                     /translation="MASYRQELEKRGADGIPSLDDVESGKLVGAVLPMSEKAQIQMRN
                     SIEVIDYLLNQERVVWS"
BASE COUNT          828 a         1036 c         1107 g          923 t
ORIGIN      
        1 ccgctgactc ccaccggcat tccacccggc ccctgaatct tgttggtcgc gtagtagacc
       61 tctttggaga gtagttcaag atagacggtg agatcggggt ctttcaggct gacctttttg
      121 ccggtttcat cgcagatggc cgcgccgacc tcccgcgcga ctcccatgga ggtcagcgtc
      181 aaacgcttgt cggctcgttt cgccgataca cgaaaggtgg aaaaggattc cgatcggagc
      241 gcctcgataa tcgccttgct gagcgcgccc aggtttggat gggccaaatc gagcggcaat
      301 ccgtgggcca gtgaaaagtt ggcgattcca aacactcgtg agagtcgtgt gataatggtc
      361 tggtcaggaa ctgcctcagg caggactacg cgaatccggc tgcgaaggtt ttcgacgcgt
      421 ctgatgccga gatctttcag cgtccattgg atattgtgta caagtcgttc ctcgaagtat
      481 tcccggttgc ggcctttgag ggcgagttcg tggtagtgga cgatcgcgca gcgcatggga
      541 ttaacaggat gctgaaaatg gccgccaacg tcgttctcgg ctcaacacaa tcctcaacgt
      601 acccctgagg ttagaggtta tgactacctg actccagaaa tcgttcagcg tcgatagctg
      661 ccatacatcc tgagcctgct gcagtgaccg cctgacgata gaccgagtcc tgcacatcgc
      721 ctgcggcaaa gacaccaggg acactggtgg cagttccctt ctccgttcgg atgtagcctt
      781 tctcatccat ggtgatttgg ccggcaaaga gcgtggtgtt tggccggtgc ccgatcgcga
      841 caaagacgcc ggcacaaggc atttccgtga ccttgccggt cacgagattt ttgactcgca
      901 cgcctgtcac cccgtcattc ccaagaatat cttcgacggc ggaattccag acgaacgcga
      961 tcttctcgtt cttcatggca cggtcttgca tgatcttgga ggcccgcagc ttatcgcgcc
     1021 ggtggacgat cgagaccttg gccgcgaact tggtcaggaa ggtcgcttcc tccattgcac
     1081 tgtcgccccc accgacaaca attagttcct tacccttgaa aaagaacccg tcgcaggttg
     1141 cacaggttga gacaccgtgt ccggtaaggc gcacctcgtt tttcaacccg atctgaatgg
     1201 ccgatgcccc ggttgcaatg ataatggttt tggtgtgcac ggtgtgttcg cagtcgatcg
     1261 tgagcgagaa cggtcccgtc ttgagatcaa ccgcactcac gtcaccggtc agaaactccg
     1321 tgccgaaccg ttctgcttgg gcccgcattt ccttcatgag ctccgggccc atgatgccct
     1381 tggcaaagcc aggatagttt tcgacctccg tcgtcgtagt cagttgtccg ccggattgcc
     1441 agccttctat gagcagaggc gagagattcg cccgtgcagc atagaccgcg gcagtgagtc
     1501 cagccggccc cgagccgata atcacgatgt ttcgcatacg ggaaaattta ccacaagggg
     1561 tgagggaatg cacggttgcg agttaggcga ggaggcgcag gctcttcaat aatcgaccga
     1621 cttgtctgcg gagtgaagtc cgagagagcg tcccatctaa tacgtggtcc gtttgttgga
     1681 tcttctttgc caacggcagt tggctgcgga tgcgtcgaag cgcttcagca cgggaaagtc
     1741 catttctttt cttgagacgc gcgagttgtg tttcctggtc agcaataacc acgatgattt
     1801 tatctacccg cttatcgata ccaacttcga agaggagagg tacgtcatag atcaccaccg
     1861 ccttcgggtc gtttcgtaca gcgtgcctta tcaatttggc ctgctggcgg gcgacgcggg
     1921 gatgaatgat ccgctcaagg cggcgtaact tgactcgatt gctaaagacg attgcaccca
     1981 gggcctggcg attgatggtg cgatcggggg tgagtacagt cttaccgaac gtgtttacga
     2041 tcgcatgcca tgcaggtttg cctggtttga cgacgtcccg cgcaagttca tcggcatcga
     2101 tgacgacggc gccgcattgc ttgaacatct tggcgacggt acttttgcct gtggcgacgc
     2161 cgcctgtgag gccgaccaca atcatgggtg cggagcataa cacgcgagtt catgcctcac
     2221 aatcaacaac tccgattgac ttctgacagg ggaggtctgt aagagattct gcatgaaaca
     2281 cgtcgcgtta ctaggtctca tcacgtcgct tgtgttctgg agtttcggcc cagggggggc
     2341 tgatgaaggg ccgcgactcg gtccccggac tctcgtggtt gcgctggatg gaaccggcga
     2401 ctacatctct cttcaggaag cggtggatgc cgccatgaaa ggcgacacgg ttttcgtcaa
     2461 ggcgggccgc tatcctcagg acgtgactat tcacagcaaa gagaaaatca agttcgtcgg
     2521 agctggagtg gatcaggtga ccatcctggg acgtgatatt gtggtgggcg cactccacgt
     2581 gggcaagtgg ccctatgggg cgacggatat tgaaatcagt gatatgagca tcaatgaccg
     2641 gggtggtcat gctgtcggct tgttcaatgg gcaagggatt gttctccgac gggtcaggat
     2701 caatggcatg ttattcagcc agcaagttca cgatgtgcgg atagaagata gtgtcatcgg
     2761 gggaagcgag acgaccggcg ctcagtttgc cgattcagaa gccgtcctca tcgggaattt
     2821 cattcatgat aatgaccatg gtgtttcgat tgcaggcaag tcgattgtgc ggttggaacg
     2881 aaacgtaatt acccgaagtc tatttgaagc cgtcgtggtg agtgatcgtg cccaagccgt
     2941 gctggtgagc aatacgttgg tcaagaatgg tggcggtgcg gcgttcctag gacaatccca
     3001 gagtgatgtc accggtaatg tcgtcgggct taatcgagtc ggttttgtga ttgctccatc
     3061 cagtcagatc agcaccgcgt tcaatgcctt ctacaacgcg gatggtgatt accttcgggt
     3121 aggtaatccg aaccaaccgg ccccggaact aagggcccag tcagatatta ccggggaccc
     3181 gcactttgtt gaccctggac atgacgattt tcgcctgcgt cttgacacgc cgcttctgaa
     3241 tattggttgt ttctcttacc tcggggccct cgaacctgcc tcaccatcaa cgccgcgatc
     3301 gacaaaaaag tagcattaaa tcaataagat gtcacactct caccagagca tggtttgcta
     3361 tgtgctatgc tacttgtaca tcgcacgcag ctgatgcgat gagtctctgg cgtaggactt
     3421 gctgatctca ttgccagaga aacaggaggc gagagtggca agttatcgac aagaactgga
     3481 aaagcgtggc gctgacggaa ttccgtcgct ggatgacgtc gagtcaggta agttggttgg
     3541 tgctgtcctt cccatgtcag aaaaggcaca gatccagatg cgtaatagca tcgaggtcat
     3601 cgattacctc ctcaatcaag agcgcgtcgt ctggagctaa cgaccgcgtt ctccgtttcc
     3661 tgaatccccc aatccaccgt cgccctcttg acagtcctct cacgccttcg tacgctcctt
     3721 gccgatatct ttattattag gagacacacc gatggcatac atgcacttgc atcgtacgcg
     3781 ttgcgtgacg atagcgaatc ggctcatgat cgccatggca ttcatgacgc tgatgccata
     3841 taccctgccg gctgctgatg agttgccgaa agagctggtc ctgccgatgg gtct
//
LOCUS       DATIPF010000258         2632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_490898, whole genome shotgun sequence.
ACCESSION   DATIPF010000258 DATIPF010000000
VERSION     DATIPF010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490898"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="VLL06_06475"
     CDS             <1..1418
                     /locus_tag="VLL06_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30645.1"
                     /translation="TTLDVVVTFDHRRALMIAPGIPAHLTLDFNLGVSNTADLTVTPP
                     VVTVKPFLVADVDPQNPKTMRLRGPLESVNQQNHTYQVFLHPMFGPLGSVPLSIFGSV
                     TVHTDATTIFEIDEVTYQGDAGLAALAAKPTGTATVANGEWKADTKRFKATEVLAGSS
                     VPGGTRDVATGVVIARSGNQLTVRGATLVRSDGTFTFRDTVTVNVGPTMKVRQQALLT
                     TGLTRDDISVGQHLTAFGTLNTSINTLDATTGLVRLLVTSVAGTVNTTGPGPLEMNVQ
                     RIQGHRITLFNFAGTGTSTAMDADPTQYQVATGALSLLGLTSGTPVRVLGFVTRFNTA
                     PPDFTAQTIVNLVDHPATLVVNWSPPTATPFSSNTDAELVLDLAGVGTTHYVWRGPVA
                     TDLLSLSVSPTIAPANPIQGLFAVGSSGQVQVFTQFHDYSLALQQRLAQGQMANVVGA
                     HGGYSDLSVTITADQIYTVLQ"
     gene            1559..1807
                     /locus_tag="VLL06_06480"
     CDS             1559..1807
                     /locus_tag="VLL06_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30646.1"
                     /translation="MRAKLARNSSVVILIQRPMIKRNLTGIVEDSGKPAIPADSLSLD
                     TLQWAFGNPRVDLSVLASMQGKCLSQLIDHESSNITDT"
     gene            2343..2612
                     /locus_tag="VLL06_06485"
     CDS             2343..2612
                     /locus_tag="VLL06_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30647.1"
                     /translation="MTFRHTAIALMFSVPLAASVSAQTVGTEVQRDVNQESRIERGLQ
                     SGQLSTGEAVPTGLHSRTQHDPRRLAPMQAIATLDQRDALFWTPG"
BASE COUNT          595 a          731 c          744 g          562 t
ORIGIN      
        1 tcacgacgct cgacgttgtc gtcacatttg atcatcgccg tgccctcatg atcgcgcctg
       61 gtattcccgc gcatctgacg ctggacttca atcttggagt ctcgaacacc gcagatctca
      121 cggtcacccc cccagtcgtg accgtcaaac catttctggt agccgacgtg gatccgcaga
      181 atccaaagac catgcgactg cgcggtcctc tcgaatcggt caaccaacag aatcacacct
      241 accaggtgtt tctccacccg atgtttggtc cgttgggtag tgttccgctg agtatatttg
      301 ggtcggttac tgtgcatacg gatgccacta cgatctttga gatcgatgag gtgacgtatc
      361 aaggagacgc ggggcttgca gccttggctg ctaagccgac gggaacggcc accgtggcaa
      421 atggagaatg gaaggccgac accaaacgat tcaaagccac tgaggtcttg gccggctcca
      481 gcgtgcctgg cgggacgaga gacgtcgcga ccggtgtggt catcgcgaga agcggcaatc
      541 aactgactgt acgaggcgcg acattggtgc ggagcgacgg cacgtttacg ttccgcgata
      601 ccgtcacggt gaacgtggga ccaaccatga aagtccgaca acaggcgctt ctgacaacgg
      661 gactgacgag ggatgacatc tcagtgggtc aacatctcac cgcgttcgga acactcaata
      721 cctccatcaa taccttggat gccacgaccg gcctggtccg gttgctggta acatccgtgg
      781 caggaaccgt gaacacgacg ggacccggtc cgctggaaat gaatgtgcag cgaatccaag
      841 gacaccgaat tacgctcttt aacttcgccg gcacgggaac gtcgacggcc atggatgccg
      901 atccgacaca atatcaggtg gccacggggg cgttgagcct cttgggactc acatccggaa
      961 cgccggtgag ggtgttgggt ttcgtgacgc ggtttaacac cgcaccaccc gatttcaccg
     1021 cgcagaccat tgtgaatctc gtggatcatc ccgccacatt agtggtgaac tggagtccgc
     1081 cgacggcgac gccattcagt agcaacacgg atgcggaatt ggtgctcgat ctagcagggg
     1141 tgggtacgac tcattacgtt tggcgagggc cggtcgcaac ggatcttctc agtctcagcg
     1201 tgtcgcccac gatcgcgccg gcgaatccaa tccagggact cttcgctgtg ggatcaagtg
     1261 gacaggtgca ggtcttcact caatttcatg attacagctt ggcgctccag cagcgcctgg
     1321 cacaaggcca gatggcgaac gttgttggag cccatggagg ctatagcgat ttatcggtga
     1381 ccatcaccgc agatcagatc tacactgtgc tgcaataagt cgactcaggc tcaggcggta
     1441 ttgtcaacgg ccccggtcga tcggaggttg atggacggac cgtacccctt cgcgccttga
     1501 gagtggtgag taccgaagga tgcgaggatg ttgctcaaaa gtctgatagg gaccaagtat
     1561 gagggccaag ctggcgcgca attcctctgt tgtgattctg attcagcggc ccatgattaa
     1621 gagaaacctc acggggatcg tcgaagatag cggaaagccg gcgattcccg cagactcctt
     1681 gagcctcgac acgttgcagt gggccttcgg caaccccagg gtggatctgt ccgtcctggc
     1741 atccatgcag ggcaaatgtc tttctcagct gatagaccac gagtcatcta acattacaga
     1801 tacttgaacg tgctacccat caatgtttcc acgatggtcc aaccattgca ctatcgtaag
     1861 tcagtgcatg cattggcgta gaggtaagtc cctgtcaaaa cccaggagga cctcaagata
     1921 gcttggttcg ccggtgcgat cctactttcg gtctctgcca ttgcgctgac ggcggtaacc
     1981 gcttattcgc acggtggtgg gctcgatgga taagggtgcc atcacgatcg taaacacgac
     2041 ggctattatt gccatcaggg gacatttgcg gggcagtcgt ttgcatcgaa aaaccagatg
     2101 ctggctgccc tggaaacact taaccaagga ccaaacgatc tgcggcaccg ggtgcaattt
     2161 gcttgtcgat cggggatgtc atctgaggcg agtacgacct tttcggtctc gcactagcca
     2221 gagaacaatt ggaatatcga atagcacaga gggcgaagaa tcactcagca ctttcatacc
     2281 ccatagtctc gtgaccgatg tttttcaacc atgcaagagc tcttgtcacc aaaggaggat
     2341 ttatgacgtt tcgccatact gccatcgcac tgatgttcag cgtcccgctt gcggcttcgg
     2401 tgtctgcaca gactgtgggc actgaggtac aacgtgatgt gaatcaggag agccgcatcg
     2461 agcggggctt acaatccggt cagctcagta caggtgaagc agtcccaacc ggtttgcact
     2521 cgaggaccca gcacgacccg aggaggctcg ctccaatgca ggccatcgcc actctcgacc
     2581 agcgcgatgc tcttttttgg acgccaggat aaccacatcc gatagcgttc ac
//
LOCUS       DATIPF010000259        11720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_755950, whole genome shotgun sequence.
ACCESSION   DATIPF010000259 DATIPF010000000
VERSION     DATIPF010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 11720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11720
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755950"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VLL06_06490"
     CDS             <1..444
                     /locus_tag="VLL06_06490"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HSS30648.1"
                     /translation="NMLYLWIFGNNIEDVMGHAKYVAFYLLCGILAALGHAFTDPSST
                     IPMVGASGAISGVLGAYVLLFPRARVLVLMPGFGATRVAAGFVLGMWFVMQLLSGGMS
                     VGREGGGVAFFAHIAGFVAGMALIGLFKRPDVPFFAPSQRDRWDG"
     gene            complement(474..2324)
                     /locus_tag="VLL06_06495"
     CDS             complement(474..2324)
                     /locus_tag="VLL06_06495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS30649.1"
                     /translation="MGSLAITSESHVEPSSEDSCDESTISRPRYVVLQSLVGIMLAYQ
                     LLSGAELIASRPTIGGIVVGLVAMVLCLWYVPHSILQASWFSGAVIGIDTVLVTATIY
                     LSGNARSELYLSYFILMLIAASVRRLSHIIGLSLLLCVGYGVILYQGVVQTGALSPGH
                     LLGVPVLLVMATFYALALQTIGAERQQKTQLLGSIETLQETERALQISRDQLEVRIKG
                     LKGDLSRANEHVRQEKKEREGLEQQLHEAQKMEAIGRIAGGIANELGHLVSMIGRQTG
                     VVLSGLKPDDPLYGPVDDIFRSGGQAAAVTAQLAGLGLHDGHVRQVLSIKAVLEEIRG
                     VMRGLLPTSIDFNMVIEDAPMDVEVDREGLEQVLLHLAVNARDAMPNGGRLLIEAKQA
                     FRGQENGPASGRGGFLPKVLIQVTDTGSGMSLETQSHMFEPFFSTKEMNVGLGLTAVY
                     GIVKRSAGHLDVVSQPGEGTVVRIALPLVRQGRPPAPSIQAHVAAKGQETVLLVEPNE
                     IDRKLALSTLLRHRYQVLEASSSVEALLLTQKHSGAVHVAVSDLMMPEISGRDLAKRL
                     LKHHPMMKPLFVSGYDDETMVSHRLNPRYLLRRPYRQAGLVEKVRELLDV"
     gene            complement(2728..3906)
                     /locus_tag="VLL06_06500"
     CDS             complement(2728..3906)
                     /locus_tag="VLL06_06500"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HSS30650.1"
                     /translation="MKAVVMEPECVLGVLGGGQLGAMFTMAARRLGYHVAVWDPDPEA
                     PAHRIATHSFSASFNDQQIFERFADLVSVVTYEWENIPAALCRALEQRKPVRPSSTIL
                     SVIQDRLEQKGFLTSNGFPVPPFAGLSVPDQLATTVQQVGYPALCKTATAGYDGKGQW
                     RIIRESDLLGAERALAESVRPGMRWIVESLVPFERELSILVVRGADGQSRTYPLVENE
                     HEEGILRMTKVPAPGSSAVAQRAATLACEVVDRLEGVGVFCLELFQVPSGELLINEIA
                     PRPHNSGHYTLDACSVSQFEQQVRVTCGMPLGEVRLLSPAVMVNLIGNDAATLMKEPS
                     CRALLDIPGAVLHLYGKRTIRPRRKMGHVTFLGETLSLALDHAKQFRDGLANGSTLSA
                     "
     gene            complement(3903..4412)
                     /gene="purE"
                     /locus_tag="VLL06_06505"
     CDS             complement(3903..4412)
                     /gene="purE"
                     /locus_tag="VLL06_06505"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HSS30651.1"
                     /translation="MLGGSKSDFPILEKAVAILTDLGIPSELTVVSAHRTPDRLFAYA
                     EQAPGRGIEVIIAGAGGAAHLPGMLAAKTHLPVIGVPIPTENLRGLDSLLSIVQMPRG
                     IPVATVAIGGAENAGLLAAQILAGRYPAIAARVKSFRKAQTDGVLQSPEAKGLVMGTK
                     GHKRPSRRS"
     gene            complement(4825..5196)
                     /locus_tag="VLL06_06510"
     CDS             complement(4825..5196)
                     /locus_tag="VLL06_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS30652.1"
                     /translation="MPSILVVDDEQQIRQLIRETLEQAGYHVTEARDGKEAVQLYRLA
                     PADVVIMDILMPDQDGLETTATLRREFPNVKVIAITGGSDMIGMLNFLDVAKMLGAHR
                     ALQKPFEMKTLLDTVQSALQG"
     gene            complement(5543..6289)
                     /locus_tag="VLL06_06515"
     CDS             complement(5543..6289)
                     /locus_tag="VLL06_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSS30653.1"
                     /translation="MHRRLEGGLPSFQRSTRAHASYSIYRRRPLLSATKTKPIRLLLV
                     DDHEVVRVGLRTVLHNHHGITVVGEAGTKIAAVQAVNRFQPDIVLMDVRLPDGSGIDA
                     ACQILASHPTTRIIFLTSYSDDNSALAAVLAGAQGYVLKNIDSSLLIRSIRAVFNGQS
                     ILNPALAHRALNWIKVWPTQAGAVRGQSLSPQEERVLALVAEGLTNKEIATTLQLSDK
                     TVKNYLANMFQKLHISRRAQAATFFVKRQA"
     gene            complement(6186..10913)
                     /locus_tag="VLL06_06520"
     CDS             complement(6186..10913)
                     /locus_tag="VLL06_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSS30654.1"
                     /translation="MSSAEHLSELEFLRLRVAELSRALAERDQSMRAPNHHLEETMQD
                     LREQSHLLRTIIEATAAETGEEFFASLVRHLTSTLHVQYALIGEVCEGRFKKIRTLAV
                     SAGGTLADNFEYELAHTPCATALTQTVAFFDRNVQATFPQFQRLADLGAESYCGVPLR
                     TKGGAVIGLLVVMDTKPLQHGDYLQSLLGILTSRIVVEFERRRAEQERAQALADLHNV
                     IETIPDIMFALDTQGNLMKWNRRLGDVTGYSPEELLNKPALAFVPPEEQPRTAAAIQQ
                     VFTEGYAELDGQLLTKDLRTIPYHWTGALLKNAQGESLGITGVGRDVSGKKRAEEELK
                     QQQRHLVDAQALAHLGSWDWDVETGEVQWSDELFRIFGYEPGAIAVTYDRFLASLHPD
                     DHASVLAAIKDAHLGTCPYDMEYRIVRPNGEVRFIYTRGDVHRDMTGHPLSMAGTVLD
                     ITERRHVEAALRASEERLALAVAGSTDILWDAHRLLGEPWYAPQTPIWWSPRVRELLG
                     IEDTEPFETLEQWAVRLHPDDKDHVFGQLAAHIEHRVPYDVEYRLRTNQGDHRWVRGR
                     GQAMWDEQGEVRRMSGSCQDITDHKWAEQALRTSEARFRTLAEETTDWMWEINERGIY
                     TYCSPKIFDLLGYEPAEALGKTPFDFMPAEEAQRVAGLFGTIAAARRPFAGIENTNRH
                     KDGRMVVLECRGVPIIDVSGAFRGYRGFDQDITDRKEAEEKLRLSEQRQELALSGASL
                     GLWDWDVSSGKTVFDTRWCSMIGYAVDELEPHVSTWQSLLHPEDVRTIQIILESHLKN
                     ERPDYEVEFRMRHKAGRWVWILARGRVVERDHAGAPLRMAGTHMDITDRKRTEDAIRE
                     AELRYRTIFMQAGAGVAQIESRTGRFVQVNRQYCEIVGLTEAEMLATTFGAITHPDDL
                     EKNLENRSRLLSGEISSFTMEKRYVRKNGSIVWVNLNVAQLWRPGEMPVHHIAIVQDI
                     TERKRAEEALQRSERQLRTVLDALPVGIWLTDQSGKPVLSNPAAKQIWAGIKHIRIET
                     AANAPGWGETIEPSNELHRWAVSHALTKGVSSLNETLDLKCLDGTKKIIRNTSVPVQD
                     EAGVVLGAIVLNEDITALRQAQEAFQLTQFSVDHAVEGFFWIGHDARILNVNNAACRM
                     LEYTRDELTTMTMYDIDPNLPSDFWSAHWKGLKQKRSMTFESKLWSKIGQVLDTEVTI
                     NYLDYEGTEYNCVIMRDIGERKRAEASLRQSEERYRFLVDNAPIGIFLSEGGRFAYVN
                     REMQRILNATRVEQLIGTPVFERIAPEFHTVVKDRIHELMDNGQPVPSLDEQYVRLDG
                     TRVDVAVTAIPASIGDRSMIQVLVLDIAERKRMEEALRQRERDLRAAVEERERISQDL
                     HDGILQSLFATGLTLETAKSMMSPRNRQMLSPSIDQVIDQLNRVMHEIRNFIAGLGLD
                     LLKGKDLTTALQHMLETLTQHHATRVRLAIDERAAQALSAEQSLHLVLVIQEAVSNCI
                     RHGRAEEATVSLKILKQGLRLSIRDNGGGFNMETAKGIGHGLSNMAARAQKIGGRFTV
                     FSKINKGTRIVFDIPKEASTVRY"
     gene            complement(11303..11386)
                     /locus_tag="VLL06_06525"
     tRNA            complement(11303..11386)
                     /locus_tag="VLL06_06525"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11351..11353),aa:Leu,seq:gag)
     gene            complement(11423..>11720)
                     /locus_tag="VLL06_06530"
     CDS             complement(11423..>11720)
                     /locus_tag="VLL06_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HSS30655.1"
                     /translation="DRPNVRYRVVEKDNARQQLMEFLDGRAGESGIVYCQSRKKVEET
                     AQWLSDEGIVALPYHAGLDADVRRRHQDRFLREDGLVMVAPDRLLIDKDFGPLL"
BASE COUNT         2565 a         3402 c         3160 g         2593 t
ORIGIN      
        1 aatatgctgt acctctggat cttcgggaac aatatcgaag acgtcatggg gcacgcgaaa
       61 tacgtcgcgt tctacctgct atgcggaatt ctagccgcct tgggccacgc ctttaccgat
      121 ccctcctcga caattcccat ggtcggagca agcggtgcta tttccggtgt cttaggcgct
      181 tatgtcctgc tgttcccgcg agcccgtgta ctcgttctga tgcctgggtt tggggccaca
      241 agggtagcag cgggttttgt cctcggtatg tggttcgtca tgcagctatt gagtggggga
      301 atgagcgtcg gccgtgaggg gggcggcgtc gcattttttg cccatatcgc cggatttgtc
      361 gctgggatgg cactgatcgg tctgttcaaa cgcccggatg tgcccttctt tgccccaagc
      421 caaagagaca ggtgggacgg ttagcgctca ccggagcgac cccacgtcgt tcatcataca
      481 tccagcaatt cacgcacttt ttccaccagg ccggcttgtc gatagggacg cctcaagaga
      541 tatcgaggat tgagacgatg agataccatc gtctcgtcgt catacccaga gacaaagagc
      601 ggtttcatca tcgggtgatg cttcaacaac cgtttggcta agtcacgccc gctaatctcc
      661 ggcatcatga gatcgctcac ggcgacatgg acggctccag aatgtttctg cgtgagtagc
      721 aacgcttcaa cagacgaact cgcctccaat acctgatagc gatgccgtaa caatgtggaa
      781 agcgcgagct tgcggtcgat ctcatttggt tccaccaaca gaaccgtttc ctggcctttg
      841 gctgcaacgt gggcctggat tgatggggca ggcggccggc cctgccgtac aagaggaagc
      901 gcaatccgca ccaccgtgcc ttcgccaggc tggctgacaa catcgaggtg gcctgcactc
      961 cgtttcacaa tgccatagac cgcagtgagt ccgagcccca cattcatttc ctttgtggaa
     1021 aaaaatggtt caaacatgtg tgactgggtt tctaggctca tcccgcttcc ggtatcggtg
     1081 acttgaatca acaccttggg gagaaatcct ccccttccac tcgcaggccc attctcctgt
     1141 ccacggaacg cttgcttcgc ttcaatcaag agccgaccgc catttggcat ggcatctcgc
     1201 gcgttcaccg cgagatgaag taagacctgt tcaagtcctt cacgatcgac ctcgacatcc
     1261 atcggcgcat cttcaatgac catgttgaaa tcgatggagg tgggcagcag gcctctcatc
     1321 actccacgaa tttcctccag cacggcctta attgagagta cctgcctgac atggccgtcg
     1381 tgaagaccca acccagccaa ttgagcggtc acagctgcgg cctgcccacc gctccgaaat
     1441 atgtcatcga ccggcccata gagtgggtcg tccggcttga gaccagatag gaccacgccc
     1501 gtctgcctcc cgatcatcga aacgagatgg ccaagctcat tggcaatgcc cccggcgatc
     1561 cgaccgatcg cctccatctt ctgcgcctca tgcaactgtt gctcaagacc ttctcgttcc
     1621 ttcttctcct gccgaacatg ctcattcgct cgtgacagat cgcccttcag acccttgata
     1681 cgaacctcca actgatcgcg cgatatctgt aatgcccgtt cagtttcttg cagagtctca
     1741 atactcccca gcaattgtgt tttttgctgc cgttcggccc caatcgtttg cagcgcgagc
     1801 gcgtagaacg tcgccatcac cagtaacacc ggcactccca gcagatgccc gggcgacaat
     1861 gctccagttt gaaccactcc ttgatagagg atgacgccat agccgacaca gagcaataga
     1921 gataacccaa taatatggga gagccggcgt actgatgcgg caatgagcat caggatgaag
     1981 tacgacagat agagttccga ccgagcatta cccgaaagat agattgtcgc ggtcacaaga
     2041 accgtatcga tcccgatcac tgccccactg aaccacgagg cctgtaagat cgagtgtggc
     2101 acataccaca ggcagaggac catcgcaacc aatcccacaa cgatgccccc gatcgttggg
     2161 cggcttgcga tcaactccgc tccagacaac aactgatagg ccaacataat gccgaccagc
     2221 gactggagca caacatatcg tgggcgagaa atcgtactct cgtcgcacga atcttccgaa
     2281 gagggctcga catgactttc agaagttatg gccagactgc ccatttgtta tgatctcctg
     2341 ttcagacaac caaaggctaa tggaccgaca ttttgaagat tctgaagagg gcttctgcat
     2401 gaggtgttct cattttctaa aacgtgccgg ctatcgaagg cctgctgtac cgggtgattt
     2461 ttcatgcagc ccggtaggtg ttgagcaagt cctgtaccga tgacacgaca aggcggagcc
     2521 atgtttttcc aatagactat gccttgaaaa gccaaatcga ttgatccatc ttcctacagc
     2581 acgtcacaaa atagaacggc acgtgatggc caaaaaatgt cttattgacc ggatgtatct
     2641 aggtagtgcg gcagaagaga cggaatcctg ttcacagcat cctgttcgtt aatactcgat
     2701 aggaacgaga tagatgaagg aaggaggcta tgcagacagg gtagagccgt tcgccagacc
     2761 atcacgaaat tgcttggcat gatcgagggc taaactgagc gtttctccaa ggaacgtgac
     2821 atgtcccatc ttgcgacggg ggcgaattgt acgtttaccg tagagatgga gtactgcacc
     2881 aggaatatca agcagagctc gacagctcgg ctccttcata agagtcgcgg catcgttccc
     2941 tattaaattc accatgacgg ctgggctcag cagacgcact tcaccaagcg gcatcccgca
     3001 cgtgacacga acctgttgct caaactggga cacgctacag gcgtcaagcg tgtaatggcc
     3061 tgagttatga ggcctcgggg caatctcatt gatgagcagt tccccgcttg gtacctgaaa
     3121 caattcgaga caaaataccc caaccccttc caaccgatcc acgacctcac atgcaagagt
     3181 ggccgctcgc tgggctacag ccgaagatcc tggagccggc accttggtca ttcgaagaat
     3241 tccctcttca tgttcatttt caaccaaggg gtatgtgcga ctctgtccat cagccccacg
     3301 gaccaccagg atcgagagtt cacgttcaaa cggcaccaag gactccacga tccatcgcat
     3361 accaggacgg acggattccg ctaacgcccg ttccgccccc agcagatcgg actctcggat
     3421 gattctccac tgtcccttgc catcataacc ggccgttgct gtcttacaga gggcgggata
     3481 cccgacctgt tggacggtgg tcgccaactg atcgggaacg ctcaggccgg cgaaaggggg
     3541 aacgggaaat ccattggagg ttaagaaccc cttttgttcg aggcgatcct gaatcacact
     3601 caggatagta ctcgacggac gaaccggctt ccgctgctcc aatgctcgac agagcgccgc
     3661 cggaatattt tcccattcat aggttacgac gctgactaag tcggcaaaac gctcgaaaat
     3721 ctgctggtca ttgaatgaag cggaaaaact gtgcgtggca atacgatgag ccggagcctc
     3781 tggatctgga tcccagacgg cgacgtgata cccgagtcgg cgcgctgcca tggtgaacat
     3841 ggcacctaat tggccgccgc ccaggacgcc caggacacat tctggctcca taaccacggc
     3901 cttcacgacc ggcgactcgg gcgcttgtga cctttcgttc ccatcaccag accctttgcc
     3961 tcaggagact ggagcacacc gtccgtctgt gccttgcgga atgacttcac acgagcggca
     4021 atcgctggat accggcccgc caggatttgc gctgcaagca aaccggcgtt ctccgcccct
     4081 ccgattgcca ccgtggcaac gggaattcct cttggcattt gcacgatgga gaggagcgaa
     4141 tcgagtccac gtagattttc ggttgggatc ggaaccccga tgaccggcag atgcgtcttc
     4201 gcagcgagca tcccgggaag atgggccgcc ccacctgccc cggcaataat gacttcgatc
     4261 ccccggccgg gagcttgttc tgcataggcg aataagcgat ctggagtccg gtgcgcagag
     4321 acaaccgtca attcgcttgg aatcccgaga tccgtcagaa tcgcaacggc cttctctaag
     4381 atcggaaaat ctgatttact tccgcccaac accccgatca agggacgcgg acgatccgtt
     4441 cctcgccgtc gtgattgtag tcctctactg cgacgagaca tctgtgcgcg aacccttctc
     4501 caaccgctct atagctctcg acggagcccc caccttagcg attctctgac tgctggtcaa
     4561 gcgcgcactc tctccacatg aaccggttct cgaagaaaac ctgaccggat aagaagagca
     4621 cctcgtccag catcctgaca ggagcctgtc cgacattgcc gtttgtcatg aggcgaatga
     4681 ggtgggggca gatgcgaggc cgcaggcccg gaaaaaccgg aggtgtgttc actggaatac
     4741 attacgtgtt ggttttggac cgtaaacaat gcagatggtc gcggatcgtt cgccgcagta
     4801 gaagggtccg tgtcggacag gcccctaccc ctgcaatgcg gattggactg tgtcgaggag
     4861 ggtcttcatt tcaaagggtt tctgtagcgc gcggtgggct cccaacattt tggcgacatc
     4921 gaggaaattg agcataccga tcatgtcact cccgccagtg atcgcgatga ctttcacgtt
     4981 cgggaattcc cgtcgcaacg tagctgtggt ctccaggcca tcctgatcag gcatgagaat
     5041 gtccataatg acgacgtcgg ctggcgccaa ccgatacagc tgaacggcct cttttccatc
     5101 acgtgcctcc gtgacgtgat agccggcctg ctccaacgtt tcacgaatca attgacggat
     5161 ttgctgctcg tcgtcaacga caagaataga tggcatcgta cctcagcctc gatgcatgac
     5221 gctggacatc agtttcccac aggctgggtc ttcaaggaaa gttttccgaa ctgtaccatc
     5281 cctacggtga gcagacaaac aaacaatgcg gcatcattac tgccgatgcc ctatcggcag
     5341 cgatgcgaac acgcgtagag agccattcat cacaagtgtc cagaggcgtg atgccctgca
     5401 gcatagctgc atgagacacg caagactggc aggacgggag tgggctgttt cattaacctg
     5461 gtcagtctgg ttggcagaat cggttcacga aagacaccgg atcgacccgc tcgtgacaca
     5521 ctgccacctt ccgtgccaag tatcaggcct gacgtttgac gaagaaggtg gcagcttgtg
     5581 cgcgtcgtga gatatgcagt ttttggaaca tgttggcgag gtaattcttg actgtcttgt
     5641 cgctgagttg cagcgtagtc gcaatttcct tgttggtcaa accttccgcc accaaagcca
     5701 gcacgcgctc ttcctgagga gaaagggatt gtccccgcac cgcgccggct tgagtcggcc
     5761 agactttgat ccaattcagc gcccgatggg cgagggccgg attgagaatc gattgaccat
     5821 tgaaaacggc acgaatcgat cggatcagca ggctggagtc gatattcttg aggacgtatc
     5881 cttgtgcacc ggccagcacg gccgccaggg cggaattatc atcggaatag gatgtgagaa
     5941 agatgattcg tgtcgtgggg tgactggcga gaatctgaca agcggcgtcg atgccggagc
     6001 catccgggag tcgaacgtcc atcaggacga tgtccggttg gaagcgattg accgcctgca
     6061 ccgcagctat tttggttcct gcttcaccga caacggtaat gccgtgatgg ttgtgcaaga
     6121 cggttcgcag gccaacacgg accacttcgt gatcgtcgac caggagcagg cgtatcggtt
     6181 tggttttagt agcggacagt agaggcctcc ttcggtatat cgaatacgat gcgtgtgccc
     6241 ttgttgatct ttgaaaagac ggtaaaccgc cctccaatct tctgtgcacg ggcggccata
     6301 ttgctcagcc catgaccgat ccccttggca gtctccatgt tgaacccacc accgttgtca
     6361 cgaatgctca accggagacc ctgtttcagt atcttcagcg acaccgtggc ctcttccgcg
     6421 cggccatgcc ggatacaatt gctcaccgct tcctggataa cgagaaccag atgcagggac
     6481 tgttcagctg aaagcgcttg cgcggcacgc tcatcgatag caaggcggac gcgcgtcgcg
     6541 tgatgctgcg tcaacgtttc caacatgtgc tgcagcgctg tcgttaagtc cttccccttg
     6601 agcaggtcta aacccaatcc cgcgatgaag tttcggattt cgtgcatgac acggttcaat
     6661 tgatcaatga cttgatcgat tgatgggctc aacatctggc gattccttgg ggacatcatc
     6721 gatttggccg tttcgagggt gaggcccgtg gcaaaaagcg actgcaaaat cccatcatgc
     6781 aaatcttggc tgatccgctc acgttcttcc accgccgcgc gcagatcacg ctctcgttga
     6841 cggagcgctt cttccatgcg cttccgctcc gcgatatcga gcaccaacac ctgaatcatc
     6901 gatctgtcgc cgatggaggc gggaattgct gtcacggcta cgtcaacccg tgtcccgtca
     6961 agcctcacat attgctcatc caaagaagga actggctgcc cgttatccat caactcgtgg
     7021 atgcggtctt tcaccacggt gtgaaactcc ggggcgatcc gttcgaagac cggcgtgcca
     7081 atcagctgtt cgacccttgt cgcgttgaga atccgctgca tctcccgatt cacgtaggcg
     7141 aatcggccac cctcgctcaa gaagatacca atcggggcgt tgtctaccaa gaaccgatag
     7201 cgttcttcgc tctggcgcag ggatgcctcc gctctctttc gctctccgat atcccgcatg
     7261 atgacgcagt tgtattcggt cccctcgtag tccagatagt tgatggtgac ctccgtatcc
     7321 aggacctggc cgatcttcga ccaaagtttg gactcgaagg tcatggaccg cttctgtttc
     7381 aatcctttcc aatgggccga ccagaaatcc gaaggaagat tggggtcaat gtcgtacatg
     7441 gtcatcgtcg tcagttcgtc gcgggtgtat tccaacatcc tgcaggccgc gttgttgacg
     7501 ttcaggatcc tggcatcgtg accaatccag aagaacccct cgacagcatg atcaacggag
     7561 aactgagtca gctggaaggc ctcctgcgcc tgacgcagcg ccgtaatatc ttcgttgagc
     7621 acgatcgcgc cgagaacgac tccggcctcg tcctggaccg gaacgctcgt gttgcgaatg
     7681 atcttctttg tcccatcgag gcatttgagg tcaagcgttt cgttcagtga agaaacgcct
     7741 ttcgttagcg catggctcac ggcccagcga tggagttcat tcgatggctc aattgtctcc
     7801 ccccagccgg gagcattggc agctgtctcg atcctgatgt gtttgattcc ggcccaaatc
     7861 tgtttggcgg cggggttaga gaggaccggc ttgcccgatt gatcggtaag ccagataccc
     7921 accggcagtg cgtccagcac cgtgcggagc tggcgttcgc tgcgttgcag tgcctcttcg
     7981 gcccgcttac gttccgtgat gtcctggaca atcgcgatat gatgaaccgg catttcgcca
     8041 ggccgccaca attgtgcgac gttcaggtta acccacacaa tgctaccatt ttttcgcaca
     8101 taacgttttt ccatcgtgaa agaggagatc tcgccggaga ggaggcgtga cctgttctcg
     8161 aggttcttct ccaaatcgtc aggatgggta atcgctccga acgtggtggc caacatctca
     8221 gcctccgtca ggcccacaat ctcacaatat tgacgattga cctggacgaa tcggcccgtg
     8281 cgcgattcaa tctgtgcgac tcccgctccg gcctgcataa agatggtccg gtaacgcagc
     8341 tcggcctccc gtatggcatc ttccgtccgc ttgcggtcgg tgatatccat gtgggttccc
     8401 gccatgcgga gcggtgcacc ggcatgatca cgttccacca ctctgccccg ggccaagatc
     8461 cacacccagc ggccagcctt gtgccgcatc ctaaattcaa cttcataatc cggcctctcg
     8521 ttcttcaggt gagattcgag aatgatttga atggtccgca catcctccgg atgcaacaga
     8581 gattgccagg tgctgacatg aggctccagt tcgtccaccg catagccgat catcgagcac
     8641 cagcgtgtgt cgaagacggt cttcccgctg gacacatccc aatcccacaa cccaaggctg
     8701 gcaccggaca gagcgagttc ctggcgctgt tcgctcagtc tgagcttctc ctccgcctcc
     8761 ttgcggtccg tgatatcttg atcaaagccg cgataccctc gaaacgcacc gctgacatcg
     8821 atgattggca cacccctgca ttcaagcacc accatccggc catccttgtg acgattcgtg
     8881 ttttcaatgc cggcaaaggg ccttcgtgca gcggcaatcg ttccgaacag gccagcgaca
     8941 cgttgggctt cttccgccgg cataaaatca aatggtgtct tgccgagcgc ttcggctggt
     9001 tcatatccta agagatcgaa aatcttggga ctgcagtagg tatagatgcc ccgttcattg
     9061 atctcccaca tccagtcagt cgtctcttcc gctaatgtac gaaatcgtgc ctcgctggtg
     9121 cgtaaggcct gctccgccca cttgtggtca gtgatatctt gacatgagcc ggacatgcga
     9181 cggacctctc cctgctcatc ccacatcgcc tgcccacgcc cgcgaaccca gcgatggtct
     9241 ccttgattgg tgcgtagccg gtattcaaca tcgtagggaa ctcgatgctc aatgtgcgcc
     9301 gcgagttggc cgaacacgtg atccttgtca tcgggatgaa gtcttaccgc ccattgctca
     9361 agcgtctcga acggttctgt gtcctctatg cccaacaatt ccctgactcg aggagaccac
     9421 cagatcggtg tctgtggtgc ataccagggc tcaccaagca gccgatgcgc atcccatagg
     9481 atatccgtcg agcctgctac agccaacgcc aaacgttctt cgctggcgcg gagcgcggct
     9541 tcgacatgtc tacgttcagt gatgtcgagg acggtaccgg ccatgctgag cgggtggccg
     9601 gtcatatccc gatggacatc cccgcgagta tagataaacc tgacttcgcc gttcggacgg
     9661 acgatacgat attccatatc gtaggggcag gttccgagat gtgcgtcctt gatggcggct
     9721 aatacagagg cgtgatcgtc cgggtgcaac gaggccagga accggtcgta tgtcaccgca
     9781 atggcccccg gctcatagcc gaaaattcga aacagttcat cggaccattg cacctcgccg
     9841 gtctcgacgt cccaatccca gctcccaaga tgtgcgaggg cttgtgcatc gacgagatgg
     9901 cgctgttgct gctttagttc ctcctccgcc cgcttcttgc cggatacatc ccgtccgact
     9961 ccggtaatgc cgagggattc gccctgtgca ttcttgagta aggcgccggt ccaatgatag
    10021 ggaatagtac ggagatcttt ggtgagcagt tgaccgtcaa gctcggcgta cccttctgtg
    10081 aagacctgct gaatggcggc cgcagtccgg ggctgttctt ccgggggcac gaaggccaag
    10141 gcaggcttgt tcaacagttc ttccgggcta tatcccgtga catccccaag acgccgattc
    10201 catttcatca ggttgccttg ggtgtcgagg gcgaacatga tatcgggaat ggtttctatg
    10261 acattgtgta aatcggccaa ggcctgggca cgttcctgct cggctcgtcg ccgctcgaac
    10321 tccacgacaa tacgtgaggt caggattccc agcaaggatt gcaaatagtc gccgtgttgc
    10381 aacggcttgg tatccatgac gacaagtaac ccaatgacgg ctccgccttt cgtccggagc
    10441 ggcaccccgc aatagctttc ggctccaagg tctgccaacc gctggaattg aggaaaggtt
    10501 gcctggacat tccggtcaaa aaaggcgacg gtctgtgtca gcgcagtcgc acaaggcgta
    10561 tgggcaagtt catactcgaa attgtcggcg agggtgcctc ctgccgagac cgcgagcgta
    10621 cggattttct tgaaccggcc ttcacacact tcgccgatca acgcatattg cacatgtaac
    10681 gtcgaggtca aatgcctaac gagtgaagca aaaaactcct ctccggtctc ggctgccgtg
    10741 gcttcgatga tggtacgcaa gagatgcgat tgctcgcgga gatcctgcat cgtctcttca
    10801 agatggtgat ttggcgctcg catcgattgg tctcgctcgg cgagtgcgcg cgagagctct
    10861 gccactcgca atcgcagaaa ttcgagttcc gacagatgtt cggcactgct catgctgcaa
    10921 ctccttgtga catacaatgg ccttgtgaga gggcaacgga cgattcatga aggggctgaa
    10981 cctggagggc actaaggcaa agacaacagg cgcatgtgct gcgatattcc atgagacacc
    11041 atgacaaact atgtgttggg tgcagatcgg gttgcaagaa ccacattgcg cccgccctag
    11101 ctggttggtt ctgcacagtc acttgggagg gcgacagtgt cctatggaag gccgtcaaaa
    11161 gtctagatcc aaatgggtgt gacgagcggg cacagaagcg cgaggggcat gagaggcata
    11221 gagtgggcta ggtgggaggg ctcccgcacg atagatgtgc gagcccacct cactgggatt
    11281 tgggatacgc gcatcgggag tttggtgccg aaggcgggac ttgaacccgc acggcttgcg
    11341 ccacacgccc ctcaaacgtg cgtgtctgcc atttcaccac ttcggcatat gaaattatca
    11401 cttcaacgac gagggagccg cattatagaa gtgggccaaa atccttgtca atcaatagac
    11461 ggtccggcgc gaccatcacg agcccatcct cgcgcaggaa gcggtcctgg tggcggcgcc
    11521 gcacgtcggc atcgagcccg gcgtgatacg gcagcgcgac gatgccttcg tcggacagcc
    11581 actgcgccgt ctcctcgacc ttcttgcgcg actggcaata gacgatgccg gattcgccag
    11641 cgcggccatc cagaaattcc atgagttgtt ggcgtgcgtt gtctttttcg acgacgcgat
    11701 agcgaacgtt cggacgatcg
//
LOCUS       DATIPF010000260         1805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_581627, whole genome shotgun sequence.
ACCESSION   DATIPF010000260 DATIPF010000000
VERSION     DATIPF010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581627"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1295)
                     /locus_tag="VLL06_06535"
     CDS             complement(138..1295)
                     /locus_tag="VLL06_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HSS30656.1"
                     /translation="MSFWQSLARPVIGMAPMDGVTDATFRHTVAMHGKPDVSFTEFTH
                     VHDVCRGPEFLLDSLIYHEVERPIIAQLYGKEPDLFYQAAHAVCELGFDGLDINMGCP
                     SRSVASSGSGAGLIRTPDVAQAIMQAARQGITDWAAGQTLEGAGLKPGRVAAIWRMNE
                     QRKPARPLVRHTIPLSVKTRLGYDVVVVDRWIQHLLMEQPAVISLHGRTLQQMYRGEA
                     DWNAIARAAEIMRGSGTLLFGNGDVHSYHDVVRRVRDTGVDGVLVGRAALGSPWFFRQ
                     KEEVRFVLQQRLPMEGPEPWVPSLDVRFEMLLDHARRFEAACGQGQFRRMRKHLGWYC
                     KGFPHAASLRADMFRVSSVTDLEQVLADFRIRVASLIEASDFSSAETSPIL"
     gene            complement(1292..>1805)
                     /locus_tag="VLL06_06540"
     CDS             complement(1292..>1805)
                     /locus_tag="VLL06_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30657.1"
                     /translation="RETVVTQLIELMPYQVPVQEPLCRSDRRQLRDDYARYAQVISGQ
                     VSVTGPEWQDVAAEASGLTRYRTSYVPHEILHWGGDLTGMFPQTCRILADRDICLTEF
                     VSFWFRSPRPLTHSYDFFLLKIERFLEFVTERVPESRVCVEEEGAMLRLAYAQANEMG
                     ESVMEMERTR"
BASE COUNT          410 a          560 c          490 g          345 t
ORIGIN      
        1 gacatgacct gttcgatcgc cggacggtcc gccaccagcc gctcctcgaa cgatgggctt
       61 ttgacgtcga ataggaggcc caacaaggca agcaactcgc ggcggcgtgg agatgtggaa
      121 gccagaatga gtgacatcta caatatcgga gaggtttcgg cagaggaaaa gtcagaggcc
      181 tcaatgagac tcgcaacacg gatgcgaaaa tcggcgagca cctgctccaa atcggtgacc
      241 gaagagactc ggaacatatc ggctcgaagc gaggcggcat ggggaaatcc tttgcagtac
      301 caccccagat gtttgcgcat tcgccgaaac tggccctgtc cacaggctgc ttcgaaccgt
      361 cgagcatggt cgagcaacat ctcgaagcga acgtccagcg acggcaccca cggttcgggc
      421 ccctccatag gcagacgttg ctgcagaacg aaccgcacct cctctttctg ccgaaagaac
      481 cagggagatc cgagcgcggc tcgcccgacc aacacgccat cgactccggt gtcccgcacg
      541 cgacggacga cgtcatgata actatgcacg tctccattgc caaacagcaa cgtcccgctt
      601 ccccgcatga tctccgccgc ccgagcaatg gcattccaat ccgcttcccc tcgatacatt
      661 tgttgcaacg tgcggccatg cagggaaatg accgccggct gctccatcag aagatgttgg
      721 atccaacgat caacgaccac cacgtcgtag ccgaggcgtg tcttgacaga caaagggatt
      781 gtatgtcgga cgagcgggcg ggcaggcttc cgctgttcgt tcattcgcca gatcgctgcg
      841 actctgcctg gcttgagccc cgcgccctca agcgtctggc cagctgccca atcggtgatc
      901 ccctgtcgag cagcctgcat aatggcctgg gccacatccg gcgttcggat gagccctgct
      961 cctgatcccg atgaggcgac actccgtgaa ggacaaccca tgttgatgtc caatccatca
     1021 aaccctaact cacacacggc atgggcggcc tgatagaaga ggtccggctc ttttccatag
     1081 agctgcgcga tgatcggtcg ctcaacctca tgataaatga gggaatccaa caagaactcg
     1141 ggtccgcgac acacgtcatg gacatgcgtg aactcggtga aggagacgtc cggcttcccg
     1201 tgcatcgcca cggtatggcg aaacgtcgca tcggtgaccc catccatcgg cgccatcccg
     1261 atgaccggtc tggcgagtga ttgccagaaa ctcatcgggt tctctccatc tccatcaccg
     1321 actctcccat ttcatttgcc tgtgcgtagg ccaggcgcaa catggcacct tcctcctcga
     1381 cgcagacccg tgactccggt acgcgctccg tcacaaactc gagaaatcgt tcgatcttga
     1441 ggagaaaaaa atcgtacgaa tgcgtgagcg gccgtggtga tcggaaccag aaggacacaa
     1501 actctgtcag gcagatgtcc cgatctgcca gaatccggca ggtctgcggg aacatcccgg
     1561 taaggtctcc tccccaatgg agaatctcgt gaggaacgta ggacgtgcga taccgtgtta
     1621 acccgctcgc ttccgcggcc acgtcttgcc actctggacc tgtcacagag acttgtcccg
     1681 aaataacttg cgcgtagcgc gcatagtcgt cgcgtaactg ccgtcgatca gatcgacata
     1741 atggctcctg gactgggact tggtacggca tcagttcaat caactgtgtg acaacggttt
     1801 ctctg
//
LOCUS       DATIPF010000261         2643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_49161, whole genome shotgun sequence.
ACCESSION   DATIPF010000261 DATIPF010000000
VERSION     DATIPF010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49161"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VLL06_06545"
     CDS             <1..886
                     /locus_tag="VLL06_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HSS30658.1"
                     /translation="IGGAILTSYWICGGAASGGLYGVAVAAVSMLSMAGIVVAIDAFG
                     PITDNAGGIAEMAHLGKEVRDITDPLDAVGNTTKAVTKGYAIGSAGLAAVVLFAEYAR
                     EVAKGSGASTFDLSNPSVLVGLFLGGMLPFIFGALCMKAVGEAAGLIVEEVRRQFRTI
                     KGIMEGTGKPEYGTCVDIVTQAAIKKMMVPGLIPVISPILVGVILGPQALGGMLVGSI
                     VTGLFVAISMTSGGGAWDNAKKYIEEQGLKGTDTHKAAVTGDTVGDPYKDTAGPAINP
                     MIKVINIVALLIVSLIIK"
     gene            1078..1275
                     /locus_tag="VLL06_06550"
     CDS             1078..1275
                     /locus_tag="VLL06_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HSS30659.1"
                     /translation="MEKQDRKQEPRREPQGKEEVKANPKVVEAGKKLKEDIDKLVDEI
                     DDVLETNAEEFVKNYVQKGGE"
     gene            1476..>2643
                     /locus_tag="VLL06_06555"
     CDS             1476..>2643
                     /locus_tag="VLL06_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HSS30660.1"
                     /translation="MKSKLWVFRDINLFQRASLAQALSISQATASLFLSRGVTTPDEA
                     TTWMSLQVPHDPFLIPDMEQAVERLYRAVTTREPICFYGDYDVDGITATSVYLSFFGG
                     LGAQVRAYVPHRLREGYGLNIGAVQRLHDEGISLLVTSDCGTTSHKEIALAAQLGMDV
                     LVTDHHQTDEDMPPAVAVLNPHRAGARYPFRGLCSAALAYKVAQAYQLRYGVAGVPLE
                     SLLDLVALATVAEVVPLHDENRSFVREGLIQLSRGSRCGVQALKQVAGVTRDCTAETI
                     AYKLAPRINAAGRLDDAMLGVRLLTTNNPAEAQQLADRLEQLNRERQRIEVDIMSEAL
                     AFLKEGALPPALVLASRRWHLGVVGIVAARLVDRFQRPTIVMAVDDQGVAKGSAR"
BASE COUNT          541 a          658 c          851 g          593 t
ORIGIN      
        1 gatcgggggt gcgattctca cgagctactg gatttgcggc ggcgcagcat cgggcggttt
       61 gtacggcgtg gccgttgcgg cggtgtcgat gttgtcgatg gccggtatcg tggtggcaat
      121 cgatgcgttc ggtccgatca cagacaatgc aggtgggatt gcggagatgg cccacctcgg
      181 caaagaggtg cgtgatatca ccgatccatt ggatgcggtt ggtaacacga cgaaagcggt
      241 gaccaagggc tatgcgatcg gatcggctgg tcttgcggcg gtggtgttgt ttgccgaata
      301 tgctcgtgag gtggcaaaag ggagtggggc gagcacgttc gacctctcga atccctccgt
      361 cctggtcggc ctctttttag gtgggatgct gccctttatc tttggggccc tctgtatgaa
      421 agcagtggga gaggcagccg gtctcattgt ggaagaggtg cgcaggcagt tccggacgat
      481 caaagggatc atggagggaa ccggtaagcc tgaatatggg acttgcgtgg atattgtgac
      541 gcaggcggca atcaagaaga tgatggtgcc gggattgatt cctgttatct cgccgattct
      601 cgtcggcgtc atcctggggc cgcaggcatt gggcggcatg ctcgtcggca gtattgtcac
      661 cggtctgttc gtcgccatct cgatgacaag cggcggcggc gcctgggaca atgccaagaa
      721 gtacattgaa gagcagggct tgaaggggac ggatacccat aaggcggcgg tgacggggga
      781 cacggtcggc gatccctata aggatacggc tggtccggcg atcaatccga tgattaaggt
      841 cattaacatt gtggcattgc tgatcgtgtc gctgatcatc aagtaggaga gacgacacag
      901 gttttacacg tgtgcgaggg attttgcgca gcgatcttct gcgaaatatg aacgagggac
      961 acgtcacggt ttccgcactg gcttgacggc caaaagaatc ctggagtaag gtaatcgtac
     1021 gggatcatgc tgctggctga tccacgagag ttccccataa taacgggagg tgctcggatg
     1081 gagaaacaag accgtaagca ggaacccagg cgagagcctc aggggaagga agaggtcaag
     1141 gccaatccga aggtcgtcga ggccggcaag aagttaaaag aagacatcga taagctggtg
     1201 gatgaaatcg acgacgtgct tgaaacaaac gctgaagaat tcgtcaaaaa ctacgtgcaa
     1261 aagggggggg aatagcccca gtcatctggt gggcctattc ccctcacttt ccttccttct
     1321 catcacactc cgttgtttac ggtccaacct ccgttcgtcc ctgacgtgcc acgtcacgtt
     1381 ttctagtttg acaacacagg gctgagagac tagtatccct ttaattctca gatagttagc
     1441 ttcagtcgag atgcgagatc gttcacgagg tccttatgaa gtccaagctc tgggtcttcc
     1501 gcgatatcaa tctctttcag cgggcttcgt tggcccaggc cctctcgatt tctcaggcca
     1561 ccgcttcact gttcttatct cgtggtgtga ccaccccgga tgaagcgacg acatggatgt
     1621 cgctgcaggt accacatgac ccgtttttga ttcccgatat ggagcaggcg gttgagcgat
     1681 tatatcgtgc cgtgacgact cgggagccga tttgcttcta tggcgattac gatgtcgacg
     1741 ggattacagc gaccagtgtg tatctgtcat tttttggagg gttgggtgca caggttcggg
     1801 cctacgtccc gcaccgtcta cgggaaggct atggactcaa catcggggcc gttcagcgac
     1861 tgcatgatga aggcatttcg ctgttggtga cgtccgattg cggcaccacc tcgcataaag
     1921 aaatcgcctt ggcagctcag ctcggcatgg atgtgctggt gacagaccat caccagacag
     1981 acgaggacat gccgccggcc gtggcggtgc tgaaccccca tcgagccggc gcccgctatc
     2041 catttcgcgg tctctgttcc gcggcgctgg cctataaggt ggcgcaggcg tatcagctgc
     2101 gctatggagt cgccggggtg ccgctggagt cgttgctcga tcttgtggcc ttggcgacgg
     2161 tcgccgaggt cgtgcccttg cacgatgaga atcgaagctt tgtgcgtgaa ggactgatcc
     2221 aattgtcacg tggcagccgc tgcggcgttc aggcgttgaa gcaggtcgca ggggtaaccc
     2281 gggattgcac ggcggaaacg atcgcctaca agctagcgcc gcgcatcaat gccgcagggc
     2341 gattggatga tgccatgttg ggtgtgcggt tattgacgac caacaatccg gccgaggcgc
     2401 agcagttggc agataggctt gagcagctga atcgggagcg gcagcgtatc gaagtggata
     2461 tcatgtcgga ggcactggcc ttcctcaagg agggagcatt gccgccggca ctcgtgttgg
     2521 cctctcgtcg ctggcatctc ggggtggttg gcatcgttgc agcgcgctta gtcgaccggt
     2581 ttcagcgtcc gaccattgtc atggctgtcg atgatcaggg tgtggccaaa ggctcagcac
     2641 gga
//
LOCUS       DATIPF010000262        20360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1201825, whole genome shotgun sequence.
ACCESSION   DATIPF010000262 DATIPF010000000
VERSION     DATIPF010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 20360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20360
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201825"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /gene="purH"
                     /locus_tag="VLL06_06560"
     CDS             <1..955
                     /gene="purH"
                     /locus_tag="VLL06_06560"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HSS30661.1"
                     /translation="KFPKILSLQFERAEMLRYGENPHQQGAFYRELHSSEPSVSRGKI
                     LHGKAMSYNNFLDANSALELAKEYDEIAVAIIKHNNPCGVALGATPVDAYVKARETDP
                     ISAFGGVIAFNRSVDLATAKEITSTFVEVVIAPGFADDALAELKRKKDLRLLDVGPLM
                     KVKQEGFDLKKLVGGLIVQDRDLGVLTEIRALQVPTVRKPTDEEYAACAFAWKVCKHV
                     KSNAIIYAKPGQTVGIGAGQMSRVDSVKLAAMKAQMPVKGCVMASDAFFPFRDGLDAA
                     AQAGITAVIQPGGSIRDGEIIKAADEHGLAMIMTGMRHFRH"
     gene            1089..2363
                     /gene="purD"
                     /locus_tag="VLL06_06565"
     CDS             1089..2363
                     /gene="purD"
                     /locus_tag="VLL06_06565"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HSS30662.1"
                     /translation="MRILIVGSGGREHAMVWALGQSPRTPTLYCAPGNAGIESLATCV
                     PIKSDDIAGLKGFVQSEKIDLTVVGPEAPLALGIVDEFRKSKLKIFGPTKAAARIEAS
                     KVFSKEIMASAKILTATAQSFDRVASALAYLDRHEVPVVVKADGLAQGKGVVVATTQE
                     EARQAVRDSMEHAVFGQAGERVLIEQFLDGEELTIMAFTDGRTVVPMLPAQDHKRLGD
                     GDTGPNTGGMGAYCPAPLGTMTLREQVTKQVLYPAVEALSRIGCPFQGVLYAGLMVVN
                     GTPYVLEFNARMGDPETQVVLPLLKTDLLDVIEAVVEHRLDQLTVNWHDETAVCIVMT
                     SGGYPGAYHTGHPIQGLPTTTDEKTMVFHAGTRRTGTEVVTAGGRVLGITGRGKTLAD
                     AQEEAYRVTRSIAFEGSHYRTDIAHRALSRHR"
     gene            2390..3046
                     /locus_tag="VLL06_06570"
     CDS             2390..3046
                     /locus_tag="VLL06_06570"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HSS30663.1"
                     /translation="MATIEDYATSTVPVLADLVRQVLGESGLIALPTESFYGLAAAPF
                     DEQALAKLWHIKGRSQGKPILLLIGDRFQLEPFVRNVPRAADVLMNAFWPGPLTIVFA
                     AARGLSNPVTAGTGSVGIRLSAWQPLVDLLRRVGPVTGTSANREGMPPPRTAEEVQDN
                     LGDAVNLIIDAGPTPGGPPSTVIDVRGPIRIIRNGSIERAVIAARLADHGLQLDTDSR
                     "
     gene            3084..3500
                     /locus_tag="VLL06_06575"
     CDS             3084..3500
                     /locus_tag="VLL06_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30664.1"
                     /translation="MIHTVMVRNLVAVAAITIVSMGLSGCDYWPPALQTQIEQLRSEL
                     QMVTMEKTQMQNQLASFSKVKDDLQAQVDDLTRANRDKSTMINSLQNSLSAAQERLLK
                     SAKAGTSKASGVKPSANVKQTAPAKKKAPAKATRTR"
     gene            3552..3743
                     /locus_tag="VLL06_06580"
     CDS             3552..3743
                     /locus_tag="VLL06_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30665.1"
                     /translation="MAVFEVPAAVFDGVLGGADAGGEVGVEAGVGIVWSEVLGVASFF
                     SPVVDAGASLPAEGFILSE"
     gene            complement(3986..5179)
                     /locus_tag="VLL06_06585"
     CDS             complement(3986..5179)
                     /locus_tag="VLL06_06585"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HSS30666.1"
                     /translation="MKLAARAGRITPSPTLAMAATAKAMAARGIDVADFSSGEPDFDT
                     PEPVKAAAEAAIRAGFTKYTPSSGIDELRQAIIDKLHAEQGLRYEKSQVLVSCGAKHS
                     LYNLAEALLESGDELIIPVPYWVSYADQTLLNDATPIFLPTREEDGYAIDSHELERLI
                     TPRTKAIILNSPCNPTGATYDRTTFERLAELALRRDLLLISDEIYEKVLYDGATHTSI
                     ASLGPEVAARTVVINGVSKAYAMTGWRIGYAAGPKDLLTAMANIQSQSTSNPCSISQK
                     AAVVALREGDAFTEKMVAEFDRRRRVIVDRLNAMPGVRCRMPSGAFYAFPNVSGLLGR
                     QSSSGTIATPTDLANYLLQGSQVAVVPGEPFGSTQHIRLSYATSMETIVRGMDRLDAA
                     FKQLT"
     gene            complement(5176..5664)
                     /gene="coaD"
                     /locus_tag="VLL06_06590"
     CDS             complement(5176..5664)
                     /gene="coaD"
                     /locus_tag="VLL06_06590"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HSS30667.1"
                     /translation="MKIGIYPGTFDPITHGHTDIITRSLRVFDKVVVAVAPNPSKHPL
                     FTLTERLEMIRIVMEKLTQVEVTHFDGLLVDYVQRYGGHAIIRGLRAISDFEHEFQMA
                     LMNRKIAKQVETVFLMPSEEYSYLSSTIIKDVANHGGSLTEFLHPEVARQLQERIRSL
                     KL"
     gene            complement(5661..6233)
                     /gene="rsmD"
                     /locus_tag="VLL06_06595"
     CDS             complement(5661..6233)
                     /gene="rsmD"
                     /locus_tag="VLL06_06595"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HSS30668.1"
                     /translation="MRVIAGSHRGRRLNGPEGTALRPTSDKVRQAIFSILGAHVAGGR
                     FLDLYAGTGAVGIEALSRGATSVTFVESDPKAVRLLRRNLQICELLDRGQIRIEKTAA
                     FLDQKECWGGPYDVLFADPPYAALKELEIVTHVWRPGLLSEHATVMIEQDSRTEMPTL
                     IDHASLVRRYLYGDTALYLYSVSTSEPSAS"
     gene            6876..7592
                     /gene="radC"
                     /locus_tag="VLL06_06600"
     CDS             6876..7592
                     /gene="radC"
                     /locus_tag="VLL06_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HSS30669.1"
                     /translation="MASKKGGLSITQWPETERPRERLLTQGPQTLTNAQLLAILLRVG
                     RHGSSAVQVGMDVLDRLGGVSGLAQCGIGELCAVPGVGEAKAAQLKAALELGKRALAG
                     PLTKGTKITSSRDLFTHYHPALRDLRHEVFKVILLDAKHAIMRDVTVSEGSLTLSIVH
                     PREVFTLAVRESAAAVIFLHNHPSGDPTPSREDRVLTARLVSAGEVLGIRVLDHLVVG
                     DGRYISFADQGWLTSQDSDV"
     gene            7676..9775
                     /gene="fusA"
                     /locus_tag="VLL06_06605"
     CDS             7676..9775
                     /gene="fusA"
                     /locus_tag="VLL06_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HSS30670.1"
                     /translation="MNEQRAKSLRNVAIISSAGAGKTSLAEAMLFAAGAVPHLGSIAQ
                     GTTVSDFEPEEMHRRSSVSTSLLHCSWNHTTINLIDPPGALSLQGETIMALRAADAVI
                     LVVRASSGIRSELTRAWSRVKALGLPCLLFLNELDNNGVSLDEVVTMCHRDLGIMPLP
                     MSLPFRNGVLLEGVLDLTQQRLMTSRTDVSTIQQLPIPDHASTVVGDARKRIQEAVAE
                     RDDRLLEQYLAAGELAQEDLINELRLGVQAGTILPLYSGSALKNIGTVPLLNAIVDFL
                     PSPMERAAKTPLEGLHPDTGEAASRRAVHDDPFSGIVFKTLIDPFVGRLSYMRVLSGI
                     LHADSTVFNSSRRSKEKSGHLYTVLGKKHTAVPSAGAGEIVAIGKLKDTQTGDTLCDE
                     HAPICYPKTPLPRPVLSFAIEPKSTADIEKISLGLHKLIEEDPTLEFSRHADTKEMVL
                     SGMGQLHIDLALEKLRRKFGAEVVLHMPKIPYRETIRATAQAQGKYKKQTGGHGQYGD
                     CWLELAPLPRGEGFKFENKVVGGAIPRNFIPAVEKGVAEAMHHGSLAGFPVVDCRVAV
                     YDGSYHAVDSSEMAFKIAGSMGFKKALETAHPVLLEPIMTVEVVAPVDYMGGVIGDLN
                     ARRGRILHVEAKDHTEQITALAPMAELLTYATALNSLTAGQGSYAMEFARYDEVPREL
                     AARIIETQKVEAHSAAH"
     gene            complement(9780..11174)
                     /locus_tag="VLL06_06610"
     CDS             complement(9780..11174)
                     /locus_tag="VLL06_06610"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HSS30671.1"
                     /translation="MMRLRQVLICLVCLAWLDIHSAGAVESAAPKPGDSPGLRLLDEI
                     QTVITDLAESAKPSVVNLAPLTAAGRGRELPQERVPNAPGSGSGVVIDPDGHIITNNH
                     VIGDATEIEVRFSDKSKLIAQVVGKDLDTDLAVLKVTADHPLPSAKFGDSSTVRVGQW
                     VLAVGNPFGLDRTVTLGVVSGIGRENINLSRYENFIQTDASINPGNSGGPLFNLRGEI
                     IGINTAIINFAQGIGFAIPSNMAKQVMEQLLTQGRVTRGWLGVSIQSLTTELARKFGV
                     KEGDGVLVNEVFDKDPAAAAGIKPGDILTRIDGTLIDTPSRLSRVVAGLLPGARTKVE
                     IIRDQQRLVLDVALIERRDRTVTTSLPQARPELKEVKIGLEVQALTPGLADQFKVRES
                     RGVLITKVDPGGLAQTEGLQEGDLIKEVNRVDVGSVEEFNGVISKVRPGETVLLRVLR
                     ESRAFYVVLRSTQP"
     gene            complement(11277..11909)
                     /locus_tag="VLL06_06615"
     CDS             complement(11277..11909)
                     /locus_tag="VLL06_06615"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HSS30672.1"
                     /translation="MSLELLGRIQQELSITGSAIYETVLALAERANRKVQVLRLHSHA
                     SSLLSQIEEVHGELGRQIAILCARRPPYSHEPPLRSDQLESVLGQAGDRVQHLKRTLL
                     NVDSQIRELKLETIHHELLTLQQDLSLRAAAIERLPLARGSPMIGRALAEVALPSSVR
                     LVTVLRGPFLVPPDQTLVLRVDDILVMIGLQEDLAQVTSEFTQARHTTLV"
     gene            12199..13848
                     /gene="pgm"
                     /locus_tag="VLL06_06620"
     CDS             12199..13848
                     /gene="pgm"
                     /locus_tag="VLL06_06620"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HSS30673.1"
                     /translation="MANHPLAGQPAPASILVNVPRLISAYYTEKPDVSMREQRVSFGT
                     SGHRGTSFKRNFNEFHIIAVTQAIYEYRKMEGITGPLYVGIDTHALSESAFATVLEVL
                     AANGVEIMVDQAGGYTPTPVISHAILAYNRDRSSGFADGIVITPSHNPPEDGGIKYNP
                     PHGGPADTQVTKWIEDRANALLAADLEGVKRVSYDQARRAGTTHRHDYLGAYLGDLPQ
                     VVNMDVIQSAKLKLGIDPLGGSGVAYWQPLAERYGLDIEIVNPAVDPTFRFMPLDWDG
                     KIRMDCSSPYAMANLIALKDRFDVAFGNDADNDRHGIVTRSAGLMNPNHYLAVSILYL
                     FSNRPGWKPGAGIGKTLVSSGLIDRVAVKLKQKLVEVPVGFKWFVSGLLDGSLGFGGE
                     ESAGASFLRRDGSVWTTDKDGIIMDLLAAEMMAKTGRNPGELYRDLTNELGEPVYERK
                     DAPATPQQKTILSKLSPDQVHATELAGDKIVAMMTTAPGNGASIGGLKVVTDHGWFAA
                     RPSGTEDVYKLYAESFRGKDHLRRIQEEAQSLITKALAAGT"
     gene            13897..14103
                     /locus_tag="VLL06_06625"
     CDS             13897..14103
                     /locus_tag="VLL06_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30674.1"
                     /translation="MDSTAVLLWGLLFGSIGFGFLLYGRKQKSVVPIIAGIALCAVPY
                     FIANVYILVTVGVVLMAIPFFVRI"
     gene            complement(14131..15753)
                     /locus_tag="VLL06_06630"
     CDS             complement(14131..15753)
                     /locus_tag="VLL06_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HSS30675.1"
                     /translation="MESLHQLQTVILLLIAVLALAAIARKLVVPYPILLVIGGLVLSF
                     IPGVPTLRLDPDIVFLVFLPPILWSAAYFTSWRDFRSNIRPITLLAVGLVLATTAAVA
                     MVAHFCLPGLGWAGAIALGAIISPPDAVSATAVAKRIGLPRRVVTILEGESLVNDATA
                     LILYRTAVVAAVSGTYTLSDTVLDFFMAAAVGVAVGLSVSVVARWALKFVEDSFSEIA
                     ITLLAPYIAWVLAEQAHSSGVLACVAGGLYLRRHFSEAVAPMTRIQARAVWDFLVFVL
                     NGVIFILIGLQLDALREAIPSAMFLPLMLSGALVSVTAIVVRLIWVPLAAWIPRLLSP
                     SLRARDPMPAWSTLFIIGWTGMRGIVSLAAALALPLTTASGAPFPFRAEIILITFSVI
                     LATLVLQGLSLAPVIRAIKLREDGSLEHEEMRAREHAATAALVRLDEIANEGWPVAEH
                     LDQMRVHYGDRVERYAKDGTVAECTAEAGEAFRRLRHETLTAERLAMIGLRNDGAISD
                     DVLHRLEHELDVEALRVGIGERRVSSGGGDPG"
     gene            complement(15868..16545)
                     /locus_tag="VLL06_06635"
     CDS             complement(15868..16545)
                     /locus_tag="VLL06_06635"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HSS30676.1"
                     /translation="MNHHNVVIISAHPDDMEIGMGGTVPKLVESMAVITSVVVTNGGR
                     SSNPFALTEQRMAEVRRGEALNAAGVLGIHDVIFCDEPDAAEEINAPAVKQKLVEILT
                     RLRPTEVYTLDEELDRHPAHRLAGKLARECVLEAGLTPSGGIWAYEIWEPFATWDRLE
                     YIDRHVAKKMAAIAEHHSQVATIPYGEGVLGLNRWRAVFADPKASAPAGMYAEVFRRI
                     TISPLST"
     gene            complement(16562..17458)
                     /gene="lipA"
                     /locus_tag="VLL06_06640"
     CDS             complement(16562..17458)
                     /gene="lipA"
                     /locus_tag="VLL06_06640"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HSS30677.1"
                     /translation="MSFIPLDQLRPSPAINRPRLPAWFKVQAKTGPDYLDIKQTMDRL
                     KLHTICEEARCPNRWECWNARTATFLILGDICTRRCHYCSVETGRPGAIDYEEPGRVA
                     TAVQALGLRHAVITSVNRDELDDGGAAIFADTIRRTRQLSPGCTIEVLIPDFEGNEAA
                     LAAVCMEMPEIVNHNIETVRRLFPAIRPQGKYQRSIELLGKAKALGMRTKSGLILGMG
                     ETIDEAREVMHDLRSVGCDIMTIGQYLQPTRQHLTVARFYDPSEFALLKEEGMAMGFT
                     HVESGPLVRSSYHAEQQAASRV"
     gene            complement(17455..18657)
                     /locus_tag="VLL06_06645"
     CDS             complement(17455..18657)
                     /locus_tag="VLL06_06645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HSS30678.1"
                     /translation="MASRVVMPKLTDTMEEGVLLAWKKREGDTVQAGEALAEIETDKA
                     VMDLEAFASGILRKILVQEGATVMSGTLIGVIGEADEDITSALTDRITAAPTASGGAQ
                     TSAPPAAGPAPASSRSQEGTRVPASPRAKALAAERGINLSTISGTGPGGRIVEEDVVK
                     AQAAAPQILPAGTDQPLSQMRKAIARATTQSKAPVPHFYLTSEIDMEQAERFRDQFKQ
                     NRISHPSVTDLLIKAAALALTRHPEINVSFTGQAIRRHAHIDIGIAVGIEDGLITPVI
                     RNCGAKSLDSISSESRSLIERARNKRLQPQEYADATFSISNLGMFDVENFIAVLIPPQ
                     AASIAVGSIRDAAIVKDGSVKVGRRMKVTLSCDHRALDGLQGAAFLKEFKRILEHPSE
                     FLPSKETP"
     gene            complement(18829..19191)
                     /locus_tag="VLL06_06650"
     CDS             complement(18829..19191)
                     /locus_tag="VLL06_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30679.1"
                     /translation="MSEHTEEKHQLAGFDARERGFSRPVVFEQADGGYHAILRYETTH
                     VVSTTQPTPGAALEELIRMLQGQGYSQLRSQLNFRKGAYLGSQEPWIEYPDPVRQSET
                     PDGLIGKFFSWFNRSTSS"
     gene            complement(19222..19578)
                     /locus_tag="VLL06_06655"
     CDS             complement(19222..19578)
                     /locus_tag="VLL06_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30680.1"
                     /translation="MSSHNHIEITETMVRLYVFLTQYLDRCLDEAARKAYPDEELHMH
                     LATARATMADILAVNPVVKSKVEKECKDVLALGAAILRGGHERASSMEPMQAQRAILR
                     NKTIALSDLLAVFRAL"
     gene            complement(19600..>20360)
                     /locus_tag="VLL06_06660"
     CDS             complement(19600..>20360)
                     /locus_tag="VLL06_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HSS30681.1"
                     /translation="LHPIVELMTMNFGILALDQIVNNAAKIHYMSGGQLSVPLVIRGP
                     GSAAHQLGAQHSQSLEAWFCHVPGLKVIAPSTPHDAKGLLKSAIRDNNPVIFIEAQLL
                     YATKGNVEDGEYTIPLGVAEVKRAGHDVTIVAYSKMLLLALEAADQLSHEGIEVEVID
                     PRTLKPMDIPTIVASVKKTGRLVIVEEGWHFCGLGAQIAESVYREAFDYLDAPIQRVT
                     GEDVPMPYSRPLEVAAIPNKGRVIEVVKFMCGTP"
BASE COUNT         4408 a         5557 c         5895 g         4500 t
ORIGIN      
        1 caagttcccc aaaatattgt ccttgcagtt tgaacgggca gagatgcttc gctatgggga
       61 aaacccgcat cagcaagggg ccttctatcg ggagctgcat tcgagcgagc catcggtgtc
      121 ccgtgggaag atcctgcatg gcaaggcgat gtcgtataac aattttctcg acgcgaattc
      181 ggcactcgag ctggcgaaag aatacgatga gatcgccgta gccattatca agcacaacaa
      241 tccctgcggt gtggcgcttg gggctacgcc ggttgacgcc tatgtgaaag cccgggagac
      301 cgatccgatt tcggcctttg gcggtgtgat cgcgtttaat cggtccgtag atttggccac
      361 agccaaagaa atcacctcca cgtttgtcga agtggtcatt gcacctggct ttgcagatga
      421 tgcgctagcc gaattgaagc gcaagaagga tttgcgtttg cttgatgtcg gacccctcat
      481 gaaggtgaag caggaaggct tcgatttgaa gaagttagtc ggcggtctga ttgtacagga
      541 tcgcgacctg ggtgtactga ccgagatacg cgccctgcaa gtgccgacgg tgcggaagcc
      601 gacggacgag gagtatgcag cctgtgcctt cgcctggaaa gtctgcaaac atgtgaagtc
      661 caacgcgatc atttatgcca aaccgggcca gacggttggg attggcgccg gtcagatgag
      721 ccgagtcgat agcgtgaagt tggcggccat gaaggcgcaa atgccggtga aaggctgtgt
      781 catggcgtcg gatgcgttct tcccgttccg ggatggattg gatgccgcgg cacaggcagg
      841 gatcacggca gtgatccagc cgggtggctc gattcgtgat ggcgaaatta tcaaagcggc
      901 agatgagcat gggttggcga tgattatgac gggcatgcgg cattttaggc attgaaatga
      961 ccgtgaatag tgatgagtga tcagttcagc gggacatgta agtccttcgc catcttcgtt
     1021 ttctgttcgt ttccgccacc agtcaacgca ccaagcatca cccacgatgc atcatccaca
     1081 ggttccttgt gagaatcctc atcgtcggca gtgggggacg cgagcatgcg atggtgtggg
     1141 cgcttgggca gagtcctcgt acacccacct tgtactgtgc accgggcaat gcgggaatcg
     1201 aatcgcttgc gacctgcgtg ccgatcaagt ccgacgacat cgctgggctg aaaggctttg
     1261 tgcagtcgga gaagatcgac ctgacggtcg tggggccgga agccccgctt gcgttgggca
     1321 ttgtcgacga gtttcgcaaa tccaagctga aaatattcgg accgactaaa gcagccgcac
     1381 gtatagaagc cagcaaagtc ttttccaaag agatcatggc cagcgcgaag attctcaccg
     1441 cgacggcgca aagttttgac cgggtcgctt ctgccttggc ctatctggat cgccatgagg
     1501 ttcccgtggt cgtgaaggcg gacggactgg cgcaaggaaa gggtgtggtg gttgcgacga
     1561 ctcaggagga agcgagacag gccgtccgcg attcgatgga gcatgccgtg tttggtcagg
     1621 ccggcgagcg tgtgttgatc gaacagtttc ttgacggcga agagctgacg atcatggcct
     1681 tcacggacgg acgaacagtg gtaccgatgt tgcccgcgca ggatcataaa cgacttgggg
     1741 atggcgatac gggacccaac accgggggta tgggcgccta ttgtcctgca ccgctcggca
     1801 ccatgacgct gcgcgagcag gtgaccaagc aggttctcta tcccgccgtc gaagcattgt
     1861 ctcgcatagg ttgtccgttt cagggcgtgt tatatgccgg ccttatggtt gtgaatggga
     1921 cgccatatgt cttggagttt aatgcccgca tgggagatcc agaaactcaa gttgtcctcc
     1981 cattgctcaa aaccgatttg ctggatgtca ttgaggccgt ggttgaacat cggctcgatc
     2041 aattgaccgt gaactggcat gatgagactg ctgtctgtat cgtcatgacg tccggagggt
     2101 atcccggggc ctatcacaca ggccacccga ttcaaggatt gcccacgacg accgatgaga
     2161 agaccatggt atttcatgca ggtacaagac gaaccggtac agaggtggtg acggccggag
     2221 gccgggtgct gggaatcaca ggacgtggaa aaactctggc agatgcgcaa gaggaagcct
     2281 atcgtgtgac ccgatcgatc gcatttgaag gttcccacta tcgaacggat attgcgcatc
     2341 gtgccctttc acgccacagg taatatggca gggggttgct aattcctgca tggcaacgat
     2401 cgaggactac gcgacttcca ccgtccccgt acttgctgat ctggtgcgcc aggtgctcgg
     2461 agaaagcggg ttgattgccc tgccgacgga aagtttctac ggacttgcgg cggctccgtt
     2521 cgatgagcag gcgctggcca aactgtggca tatcaaaggc cggtcccaag gaaaaccgat
     2581 tctcctgctg atcggggaca ggtttcagtt ggagccgttc gttcgaaacg ttccccgtgc
     2641 agcagacgta ttgatgaacg cattctggcc cgggcctctc accattgtgt ttgccgcagc
     2701 gcgtggactc tccaatccgg tgactgccgg caccggctct gtcggcattc gcctcagtgc
     2761 ctggcagccg ctggttgatc tcttgcgtcg agtggggccg gtgaccggta ccagtgcgaa
     2821 tcgtgaaggc atgccgccac ccaggaccgc cgaagaagtc caggacaacc ttggagacgc
     2881 ggtcaatctg atcatagatg cggggccgac ccctggcggg ccaccgtcca cggtcattga
     2941 cgttcggggt ccaatcagaa ttattcgcaa tggatcaatc gaacgagccg tcattgcagc
     3001 acggctggct gatcacggcc tgcagctgga cactgatagt cggtgaagtc gattatatcg
     3061 tgactatgga aaggagaacg ttgatgattc acacagtgat ggtgcgtaac cttgttgcgg
     3121 tcgccgccat aacgattgtt tcgatgggtc tgtccggatg cgattactgg cccccggcgc
     3181 tccagacaca gatagaacaa ctgcgctcgg aactccagat ggtgacaatg gaaaagaccc
     3241 agatgcaaaa tcaactggcg tccttttcga aggtcaaaga cgatttgcag gcgcaagtag
     3301 acgatctcac gcgggcgaat cgcgataagt cgaccatgat taacagtttg caaaatagtt
     3361 tgtccgcagc acaggaacgg ctgttgaagt ctgcgaaagc cggaacatca aaggcgagcg
     3421 gtgtcaagcc gtccgcaaat gtcaagcaga cagccccggc caagaagaaa gcaccggcaa
     3481 aggccacacg cacacgctag cctgcgtttg tctctactgt ctatttggtc gtagaagacg
     3541 caggcgcggt cgtggctgtg tttgaggtac cggcggcagt gttcgacggg gtgcttggcg
     3601 gggcagatgc gggaggagag gtcggggtcg aggcaggcgt aggtattgtc tggtcggagg
     3661 tgcttggtgt tgcctctttt ttttccccag tcgtggatgc cggtgcttca ctgcctgcgg
     3721 agggcttcat tttgtcggag tagtccgtca cgtaccatcc gcttcccttg aacatgatgg
     3781 caggcgagga aatgagtcga cggacctgct tcccgcaccg ggggcaggcg gtgaggggat
     3841 cgtccttcat gctctgcttg atctcaaact tctccgtgca gacatcacac tggtattcgt
     3901 agataggcac gttttatctc ctcctgctac aacggaatcc cgtcgtgtct acgtggattg
     3961 gccccatatg aggtaggccc catatctagg taagctgttt gaatgcggcg tcaaggcgat
     4021 ccattcctcg gacaatcgtc tccatgctgg tcgcatacga cagtcgaatg tgctgggtgc
     4081 ttccaaacgg ctcaccggga accaccgcaa cctgagatcc ctgcaacagg tagttagcaa
     4141 ggtcagtcgg agtggcgatg gttccgcttg aactctgccg tccgagcaac ccactcacgt
     4201 tgggaaacgc ataaaatgct ccactgggca ttcgacagcg aacgccaggc atcgcattaa
     4261 ggcgatcgac tatgacgcgc cggcgtcgat cgaattccgc gaccatcttt tccgtaaatg
     4321 catcaccttc gcgcaaggct acaacggcag ccttttggga gatcgaacag gggttggagg
     4381 tactctggct ttgaatgttt gccatcgcgg ttaacaggtc tttcggcccg gctgcatagc
     4441 cgatgcgcca gccggtcatc gcataggcct tcgagacgcc attgatcacg acggtacggg
     4501 ctgcgacctc cggccctaac gaggcgatgc tggtgtgtgt cgctccgtcg tagagaacct
     4561 tttcataaat ttcatctgaa atcagcagga gatcacggcg gagagccagc tcggcaaggc
     4621 gttcaaacgt cgtgcgatcg taggtcgctc cggttggatt gcacgggcta ttgaggataa
     4681 tcgccttcgt acgcggtgtg atgagccgtt ccaactcatg ggaatcaatg gcgtacccat
     4741 cctcttcccg ggtcggcaag aaaatcggcg tggcgtcgtt cagcaaggtt tggtcggcat
     4801 aggagaccca atagggaacg ggaatgatca gttcgtcgcc cgattcaagg agtgcttcag
     4861 caaggttata gagcgaatgt ttggcgccac aggagaccag cacttgagat ttctcgtagc
     4921 ggagtccttg ttctgcatga agcttatcga taatcgcttg gcgcagttca tcgatgccgg
     4981 atgagggcgt gtacttcgta aatcccgcac gaatggcggc ttcggcggcg gctttgaccg
     5041 gttctggagt gtcaaagtcc ggttctccgg atgagaaatc ggccacgtcg atgccgcgtg
     5101 cggccatggc ctttgcggtt gccgccatgg ctaaggtggg ggagggggta atgcgccccg
     5161 ctcttgcagc aagcttcata gtttcagact cctgatacgt tcctgcagtt ggcgcgcaac
     5221 ttcggggtgg agaaactccg tgagcgaccc gccgtggttg gccacgtctt taatgatggt
     5281 cgacgagaga taggagtatt cttcgctggg catgaggaat acggtctcaa cctgtttggc
     5341 gattttccga ttcatgaggg ccatctgaaa ttcatgttcg aagtccgaaa tcgcccgcaa
     5401 accacgaata attgcatgac caccgtatcg ctgaacataa tccacaagga gaccatcgaa
     5461 atgggtcact tcgacctgtg tcagcttctc catcacgatg cggatcatct ccagccgttc
     5521 ggtcaaagtg aagagtggat gcttggacgg attgggtgcg acggccacga caactttgtc
     5581 aaacacgcgc aagcttcggg tgatgatgtc ggtgtgcccg tgggtaatgg ggtcgaacgt
     5641 tcccggatag ataccaattt tcatgacgcg ctcggttccg aggtcgagac actatataaa
     5701 tagagtgctg tatcgccata caggtatcgt cgcacaagcg aggcatggtc gatcaatgtc
     5761 ggcatctcag tgcgggagtc ttgttcgatc atcaccgtcg catgctccga caacagaccg
     5821 ggcctccaga catgggtcac aatttccagt tccttcagcg cggcataggg cggatctgca
     5881 aacagtacgt cgtagggacc accccagcat tctttctgat cgaggaacgc ggcggtcttt
     5941 tcgatacgaa tctgaccccg gtcaagtagc tcacagatct gtagatttct ccgtaacagc
     6001 cgcacggcct tggggtctga ttcgacaaat gtgacggatg tggctcctcg gctcagtgct
     6061 tcgatcccga cagcgcccgt acctgcatac aaatcaagaa agcgacctcc tgctacatgg
     6121 gctccgagaa ttgaaaaaat ggcctggcga actttgtcag aggtgggacg cagtgcagtc
     6181 ccttctggcc catttaagcg gcgtcctcgg tgagacccgg caataacacg catactttca
     6241 ggtgagagac atttctagag cagatgaatc taacatagcg tttttgaagg ggtcaaggtg
     6301 actgtggtga aacgaggtgt gagcgtgcga agtggcgccg atgtgggaat gagaaaagct
     6361 ggaaaaaagg aatggaagtg ttttgacgac tttcgagctt gtgcctataa tgccgcctgg
     6421 tgatccagtg gcgcaataca tgagggacgc attagtgaga cacggtcgac catccatcga
     6481 tgttgagagg gtcgagtact ctatagatca ttcgcacgta ttctatgggg tcgccactgc
     6541 ctgcggttta cgagcggcca agctcttccg ccggttattt gaaatggtgc gatcaatgat
     6601 ggctccgcag ttgatgcatt tccacgcgta gaaaattagg aagaaatctg agaatcgctc
     6661 aagcatcatg aggcctttgc acttcggaca ttccattgaa tcctcccagg gtggctgtgt
     6721 actgctgcgg cactccaaag caattactgc accaatttgg tgccgtacgt aatttcaaca
     6781 gtttagaggg tacgtatttg ttagcaaggg ccaccggggg aggaagctct agtacgaaaa
     6841 cgttcagagt gtcacttttt gtgcagaggg gatgaatggc cagcaagaaa ggaggactaa
     6901 gcatcacgca atggcctgaa acagaacggc cgcgcgaacg attgctgacc cagggcccac
     6961 agacattgac taatgctcag ctcctcgcca ttctcctgcg ggttggtcgt cacgggtctt
     7021 cagccgtaca agtcgggatg gatgttctgg atagactcgg aggtgtctcc ggacttgcgc
     7081 aatgcggaat tggagaactc tgcgcggttc caggtgtggg ggaggccaaa gccgctcaat
     7141 tgaaggccgc gcttgaattg gggaagcgcg cccttgctgg cccgctgaca aaaggaacaa
     7201 aaattacatc gagccgtgac ctctttacgc attatcatcc ggccttgcgc gacctgcgtc
     7261 acgaagtctt caaagtcatt ttactggatg ccaagcatgc catcatgcgt gacgtgacag
     7321 tatccgaagg cagtttgacg ctcagcattg tccatcctcg cgaagtcttt accctcgcgg
     7381 tacgagaatc cgctgcggca gtcatcttct tgcataatca tccgagcggg gatccgacgc
     7441 cgagccggga agaccgtgtg ctgacggcac gattggtatc ggcaggggag gtgctgggga
     7501 ttcgggttct tgatcatctc gttgtcggcg atggacgcta tataagcttt gcggatcagg
     7561 gctggctgac gagccaggat tccgacgtat gaccgaaggg agagtcgcga tgtaatcagg
     7621 ctattttact gccggaagat gtgaccgtct tccggttttc atgaggaggg accccatgaa
     7681 cgaacaacgc gcgaagtcac tcagaaatgt ggccatcatc tccagtgccg gtgccgggaa
     7741 aacctcgctg gctgaagcca tgctctttgc cgccggcgct gttccccacc tcgggtcgat
     7801 cgcacaaggt acgaccgtct cggacttcga gccggaagaa atgcaccggc gcagttccgt
     7861 cagtacgagt ctcctccatt gttcctggaa tcacaccacc atcaatctta tcgacccgcc
     7921 cggcgcactc agtttgcagg gggaaaccat tatggcgctc cgggcagcgg atgccgtaat
     7981 ccttgttgta cgtgcctcat cggggattcg gagcgaactc accagagcct ggtctcgagt
     8041 caaagccctc ggtttgccct gtcttctgtt cctcaacgaa ctggacaata atggggtctc
     8101 cctcgacgag gttgtcacga tgtgccaccg cgatcttgga atcatgccat tgccgatgtc
     8161 gttgccattc agaaatggtg tcctgcttga gggggtgctg gatcttactc aacagcgcct
     8221 catgacttct cgtacggacg tgtccacgat tcagcagctg ccgatacctg accatgcaag
     8281 tacggtggtg ggggatgcgc ggaagcgcat tcaggaggcc gttgccgagc gtgatgatcg
     8341 gttactggaa cagtatctcg ctgcggggga attggcacag gaagacctca tcaatgaact
     8401 tcgccttggt gtccaagccg gcacgattct tccgctttac agtggttcgg ctctcaagaa
     8461 tatcgggacc gtcccgcttc tgaatgcgat cgtcgatttc ctgccctcgc cgatggagcg
     8521 ggcggcgaaa actccgctgg aaggcctgca tccggatacg ggagaagcgg cgagccggcg
     8581 tgccgttcat gacgacccat tttcagggat cgtcttcaag acgttgattg acccgtttgt
     8641 cggacgactg tcgtatatgc gggtcttgtc gggcattctt cacgcagatt cgacggtctt
     8701 caacagttcc cggagaagca aagagaaaag cggacatctc tatacggtgt tggggaaaaa
     8761 acatacggcc gtaccttcgg caggagctgg cgagatcgtg gcgattggga aattgaaaga
     8821 tacgcaaacc ggtgacacgc tctgtgacga acatgccccc atctgttatc cgaagacgcc
     8881 gcttccgcgg ccggtgttgt cctttgcgat cgagccgaaa tccacggccg atatcgaaaa
     8941 aataagtctc ggcctgcata agttgataga agaagatccc acgcttgaat tttcgcgaca
     9001 tgccgacacc aaagagatgg tgttaagcgg gatgggccag ttgcacatcg atcttgctct
     9061 cgaaaagctg cgacgaaaat tcggcgcgga agtggtgctt catatgccga aaattccgta
     9121 tcgcgaaacc attcgtgcca cggcacaagc ccaggggaaa tataagaagc aaacaggagg
     9181 acatggccag tacggtgatt gctggctgga gcttgcaccc ctgccgcgcg gagaagggtt
     9241 caagtttgag aacaaggtcg taggcggagc gattccacgc aattttattc cggcggtgga
     9301 gaagggggtg gctgaagcca tgcaccatgg gagtttggcg gggtttcccg tcgtggattg
     9361 ccgggttgcc gtgtatgatg ggtcgtacca tgcggtggat tcatcagaga tggcgttcaa
     9421 gattgcgggg tcgatggggt tcaagaaggc gttggagacg gcccatcccg tcctgctgga
     9481 acccatcatg accgtagaag tcgtggcgcc ggtggattac atgggggggg tcatcgggga
     9541 cttgaatgcg cgacgagggc ggatcctcca tgtggaggca aaggaccata ctgagcagat
     9601 cacagccctt gcccccatgg cggagctgct cacatacgcc acggcgctga attccttaac
     9661 ggcaggacag gggagttatg cgatggaatt tgcgaggtac gacgaagtgc ctcgtgaact
     9721 tgcggctcga atcattgaga cccagaaagt ggaagctcac tcggccgcgc attgaacgat
     9781 cagggctggg tcgatctgag caccacgtag aaggcgcggc tttctctcag cacacgaagc
     9841 agaacggtct cgccaggtct gaccttggag atcacgccat tgaattcttc gaccgatccc
     9901 acgtcgacac ggttcacttc cttgataaga tctccttcct gtagtccttc cgtttgggca
     9961 agcccaccgg ggtctacttt ggtgatgagg acgccccgac tctcccggac cttgaattga
    10021 tcggcaaggc caggcgtcag ggcttgtacc tcaaggccta tcttgacctc cttgagttca
    10081 ggacgtgcct gcggaagaga ggtggtcaca gtgcggtcgc gacgttcaat aagggcgaca
    10141 tccagcacca ggcgctgctg atcgcgaata atttcgacct ttgtcctcgc accagggagc
    10201 aatcctgcca cgacgcgaga aagccgactc ggcgtgtcga tcagggtgcc gtcgatacgg
    10261 gtcaggatgt cgccgggttt gataccggct gcagcagcgg ggtccttgtc aaagacttca
    10321 ttcaccaaca ccccgtcgcc ctccttgacg ccgaacttgc gtgccagctc cgttgtgagt
    10381 gattgaatgc tgacacccag ccagccccgt gtcacacgtc cctgagtcag caattgctcc
    10441 atcacttgct tggccatgtt ggagggaatc gcgaacccaa tcccctgggc gaagttaata
    10501 atggcagtat tgatgccgat gatttcgcca cgcaaattga agagcgggcc gccggagttg
    10561 cccggattga tcgacgcatc cgtctgtatg aagttttcgt agcgcgaaag attgatgttc
    10621 tccctaccga tcccgctgac gaccccgagc gtgactgtcc gatcgagtcc aaaaggattc
    10681 cccacggcga ggacccattg gccgacacgt acggtcgatg agtctccgaa ttttgcgctg
    10741 gggagaggat ggtcggcggt caccttgagg acggctaaat ccgtatccag atctttcccg
    10801 acgacctgtg caatcagttt ggatttatcg gaaaatctga cctcaatttc tgtcgcgtct
    10861 ccgatgacat gattgtttgt aataatgtgt ccgtccggat cgatcacgac accggatccg
    10921 gatcctgggg cattgggtac ccgctcttga ggcaattcgc ggcctcgtcc ggctgcggta
    10981 agcggtgcga gattgaccac ggagggtttg gccgattcgg ccagatcagt gatgaccgtt
    11041 tgtatttcat cgaggaggcg gagacctggc gagtcgcccg gctttggcgc ggcggactca
    11101 acggcgccag ctgagtgaat atcgagccag gccaagcaca cgaggcatat gagaacctgt
    11161 cgcagccgca tcatccgttg gctaacctcc catacaagca ttttatctca gagaaggcgg
    11221 agaagcctac tccgtctgaa agttcattca gtagaaaagc aggttgtcgc actgatttac
    11281 accagtgtcg tatgtcgagc ttgagtgaac tctgaggtaa cctgtgcgag atcttcctgc
    11341 aatccgatca tcacgaggat atcatcgacg cgaagtacaa gggtttggtc aggtggtacg
    11401 agaaatggtc cgcgaaggac tgtcaccaga cgaactgatg agggcagtgc cacctccgca
    11461 agtgctctcc cgatcatcgg tgatccccgg gcgagaggaa gccgttcgat ggcagctgcg
    11521 cgaagactca ggtcctgttg cagggtcaac agttcgtgat ggatcgtttc cagcttgagc
    11581 tcgcgtatct gactatcgac gttgagcagt gtccgtttca gatgctgaac ccggtcgccg
    11641 gcctgaccaa gaaccgattc caactgatcg gatcgtagtg gcggctcatg gctgtagggt
    11701 gggcgtctcg cacagaggat ggcaatctgg cgtcccagtt cgccatgcac ttcttcgatc
    11761 tggcttaaca aggatgatgc gtggctgtgc aatctaagga cctggacctt gcggttggca
    11821 cgttcagcga gcgcaagaac ggtttcataa attgcgctac cagtgatcga cagttcctgc
    11881 tgtatacgac cgagcagttc gagggacata aaagtaaatt ccgcaatgtc tgataagaac
    11941 tattatgtca acttacatgt taatcacatg acgcatgtac cctcacctgc ttcacctccc
    12001 tttactcttc ttattcaagg aagtaaatct ctttaccact ttgacacaca tcttacgacc
    12061 cactcattca ttgaatgcga tgcactcgaa ccggagatct cttctccata tacgttatct
    12121 gcccttgaca gtatgcctct cgcaggcata cacagcggca ggtctattgt ttccccgcct
    12181 caaccaaagg attagatcgt ggccaatcat ccgcttgcag gtcaaccagc accggcatcg
    12241 atcctggtta acgtcccgcg tctcatcagt gcctactaca cagaaaagcc cgatgtaagt
    12301 atgcgtgagc agcgtgtcag ttttggcaca tccggacatc ggggcacatc cttcaagcga
    12361 aactttaacg aatttcatat tattgctgtc acgcaagcga tttacgaata ccgcaagatg
    12421 gagggcatca ccggcccact ctacgtcggt atcgacaccc acgcgttgtc tgaatcggcc
    12481 tttgccacag tcttggaagt gttggccgcc aatggcgtcg agattatggt ggaccaggct
    12541 ggtggctata caccgacacc tgtgatttcc cacgcgattc ttgcgtataa ccgtgacagg
    12601 agttccggat tcgccgacgg catcgtcatc actccgtcgc acaacccgcc ggaggacggc
    12661 ggcatcaagt acaaccctcc gcatggcggg ccggccgata cccaggtcac gaaatggatt
    12721 gaggatcgcg cgaatgcgct ccttgccgct gacctggaag gcgtgaagcg tgtgtcgtac
    12781 gaccaggctc gtcgtgcggg cacgactcac cggcatgatt accttggcgc ctacctcggt
    12841 gatctcccgc aggtcgtcaa tatggacgtg attcaatcgg ctaagttgaa gctcggcatc
    12901 gatccactcg gcgggtccgg cgtggcttac tggcagccac tggcagagcg gtatggtttg
    12961 gacattgaga tcgtaaaccc cgccgtcgat ccgactttcc ggttcatgcc gctcgattgg
    13021 gacgggaaga ttcgtatgga ctgttcttcc ccttatgcga tggcgaatct catcgcccta
    13081 aaagatcgct tcgatgttgc gtttggcaac gatgcagaca acgaccggca tggcatcgtc
    13141 actcgatcag ccggcctgat gaaccccaat cactatctcg ccgtctccat tttgtacctc
    13201 ttttccaacc gtccggggtg gaagcccggt gccggtatcg ggaagacgct cgtgagcagc
    13261 ggcctcattg atcgtgtagc ggtgaagctt aagcagaagc ttgtcgaagt cccggtcggc
    13321 ttcaaatggt ttgtgagtgg attgctcgat ggttcgctcg ggttcggcgg agaagaaagc
    13381 gcgggcgctt cgtttcttcg tcgagatgga tcggtctgga ccactgacaa ggatggtatc
    13441 atcatggatc tgttggccgc ggagatgatg gccaagaccg gccgaaatcc tggcgagctg
    13501 taccgagacc tgaccaacga actcggcgag ccggtgtacg agcggaagga tgcacccgcg
    13561 acaccgcaac agaaaacgat cctctcgaaa ctgtcaccag atcaagtcca cgccaccgaa
    13621 ttagccggtg acaagatcgt cgcaatgatg accaccgcgc caggcaacgg cgcgtccatc
    13681 ggtggactca aagtggtgac tgaccacggc tggttcgccg cccgcccctc cggcaccgag
    13741 gatgtgtaca aactctatgc tgaaagcttc agaggaaagg accatttgcg gcggattcag
    13801 gaagaagcac aatcattgat cactaaagca cttgctgcag gcacgtgaga aggagcgatc
    13861 cctttcatcc tggcatgtgg gtcatgaatg tttgagatgg acagcacagc ggtcttgctt
    13921 tgggggttac tgttcggatc aatcgggttt ggatttttac tctatgggag aaagcaaaag
    13981 tcagttgtgc cgatcatcgc cgggattgca ctctgtgccg tcccctattt cattgccaac
    14041 gtgtatatac ttgtcacggt cggagtggtc ctcatggcca tcccgttctt tgtgcgaata
    14101 taaagcgaca tgacagcctg ccaggtttcc ctacccggga tcgccgcctc cggatgaaac
    14161 ccgcctctcg ccgattccta cacgcaacgc ctcaacgtcc aattcgtgct cgagacggtg
    14221 cagcacatca tcgctgatag cgccgtcgtt ccgcaggccg atcatggcaa ggcgctctgc
    14281 cgtcagcgtc tcatgccgca accgccggaa tgcctcaccg gcttcggccg tacattcagc
    14341 taccgttcca tccttggcat atcgttcgac gcggtcacca tagtgcacgc gcatctgatc
    14401 gagatgttcg gcaacgggcc acccttcgtt tgcgatctcg tcaaggcgaa cgagcgcggc
    14461 cgtcgcggca tgttcccgtg cccgcatttc ctcatgttcc aaactcccgt cttctcgtaa
    14521 ttttatcgca cggatcacag gggcaagtga caagccctgc aacactaatg tggcgagaat
    14581 caccgagaag gtgatcaaga tgatctctgc cctaaaggga aacggcgccc cggacgccgt
    14641 cgttagcggc agcgccaagg ccgctgcgag agaaacgatc ccacgcatac cggtccagcc
    14701 gatgatgaac agcgtcgacc acgccggcat cggatcgcgc gcccgcagcg aagggctgag
    14761 caatcttgga atccatgcgg ccagcggcac ccagatcagg cgcaccacga tcgccgtgac
    14821 gctcacgagt gccccgctga gcataagagg gaggaacatg gcagatggga tggcctcacg
    14881 aagcgcatcg agctgcagac cgatcagaat aaaaatgacg ccattgagta cgaacacgag
    14941 gaagtcccac acggccctcg cttgaatcct ggtcatcggc gcgacggctt cactgaaatg
    15001 tcgccgaaga tagagaccgc cggcgacgca agccaacacg cctgacgagt gagcctgttc
    15061 agccaagacc caggcaatgt acggtgcgag gagcgtgatg gcaatctcgc tgaaactatc
    15121 ctcgacgaat ttcaatgccc agcgagccac cacgcttacc gagagtccaa cagcgactcc
    15181 cactgccgcc gccataaaga agtccaggac ggtgtcgctc aatgtatagg tgccactgac
    15241 cgccgccacg accgcggtgc gatacagaat cagcgccgtc gcatcgttta cgagactctc
    15301 cccctccaga atcgtgacca cgcggcgtgg gaggcctatt cgcttcgcga ccgctgtcgc
    15361 cgatacggca tcgggaggtg aaataatcgc ccccagcgca atcgctccag cccagcccaa
    15421 tcccggcagg cagaagtgtg caaccatcgc gactgcggcg gtggtcgcca atacgagtcc
    15481 aactgctaac aaggtaatcg ggcgaatgtt tgaacggaaa tcgcgccacg aggtgaagta
    15541 ggcagccgac cagagaatag gaggaagaaa gaccaggaac acaatgtcgg gatcgagccg
    15601 cagcgtcggc acgccgggaa tgaagcttaa gaccaaccct ccgatcacca gcaagatggg
    15661 gtaagggacc acgagtttgc gtgctatcgc ggcaagggcg agtacggcaa tcaacagcag
    15721 aataacggtt tggagctgat ggagactttc catatcgcgc tcatggtacg cgaatcgtag
    15781 gtgcaaagaa cagggacatc ctggacaatg cccgttcgga ccgccattcg tatgcaggag
    15841 acaaaaggat gggtgacgaa acggagtcta tgtagaaaga ggagaaatcg tgatccgtcg
    15901 aaagacttcc gcatacatgc ctgccggggc gctagccttg ggatcggcga atacggctcg
    15961 ccagcgattc aatcctagga ctccttcccc ataggggatg gtggccacct gactgtgatg
    16021 ctccgcaatg gcggccatct ttttggccac atggcggtcg atatactcaa ggcggtccca
    16081 agtcgcgaac ggttcccaaa tttcataggc ccagatgccc ccgcttgggg tgaggccggc
    16141 ttcaagcacg cactcccgtg ccagtttgcc ggcaagacga tgggccggat gtcggtcaag
    16201 ctcctcatcc agcgtataca cttcagttgg ccgaaggcga gtgagtatct cgacgagctt
    16261 ctgtttgaca gccggagcat tgatctcctc cgctgcgtcc ggttcgtcac aaaagatcac
    16321 gtcgtgtatg cccaggacgc ccgcagcatt aagggcctcc cctcttctca cttccgccat
    16381 ccgctgctcc gtcagcgcga agggatttga tgaccgaccg ccgtttgtca cgacgacgga
    16441 ggtaatcaca gccatggatt ccaccagctt ggggactgtt ccgcccatgc cgatttccat
    16501 gtcgtccgga tgcgcgctga tgatgacgac gttatggtga ttcatggctg aagcatactc
    16561 ctcacactct ggaggctgcc tgctgttcgg cgtggtagga actgcggacc agcgggccgg
    16621 attcgacatg ggtgaaaccc atggccatcc cctcctcttt taagagggca aactcgctgg
    16681 gatcgtagaa gcgtgcgacg gttagatgct gtcttgtcgg ttgaagatat tggccgatgg
    16741 tcatgatgtc acagccgacg gagcggagat cgtgcatgac ttcgcgggcc tcgtctatgg
    16801 tttctcccat cccgaggatc aacccggact tggtgcgcat gcccaatgcc ttcgccttac
    16861 ccagtaactc gatcgaccgc tgatatttcc cttgtggtct gattgccgga aacagtcgtc
    16921 ggacggtttc gatgttgtga ttcacaatct caggcatctc catacagacg gctgcaagcg
    16981 ccgcctcatt cccttcgaaa tcaggaatca agacttcgat cgtgcagcct gggctgagct
    17041 gtctcgtccg ccggatcgtg tcggcaaaga tcgcggcgcc gccatcatcg agctcatccc
    17101 gattcaccga tgtgatcacg gcatgccgaa ggcccaacgc ctggaccgcg gtggcgacgc
    17161 gaccgggctc ttcataatcg atcgcccccg gccggcctgt ctcgaccgaa cagtagtggc
    17221 agcgtcttgt gcagatatcg ccaagaatca agaaggtagc ggtgcgcgcg ttccaacatt
    17281 cccaccgatt cgggcagcgg gcttcttcgc aaatggtatg gagcttgagc cggtccatcg
    17341 tctgtttgat gtcgagataa tccggcccgg ttttcgcttg aaccttgaac caagccggga
    17401 gacgaggacg gttgatagcg ggagaaggtc gaagctggtc gaggggaata aagctcatgg
    17461 agtctccttt gacgggagga actccgatgg gtgttcaagt attcgtttaa attccttcag
    17521 gaacgctgcc ccttgcaggc catcgagagc cctgtggtcg caggataacg tgaccttcat
    17581 gcgccgcccc accttgactg aaccatcctt gacgatagcc gcgtcgcgga tgctacccac
    17641 agcgatagat gctgcctgag gggggatgag gactgcaatg aagttttcca cgtcgaacat
    17701 tcccaagttt gagatcgaga aggtggcatc cgcgtattcc tgcggctgca ggcgtttatt
    17761 cctggcccgt tcgatcagcg accgcgattc gctcgagatg ctatcgagcg atttcgcgcc
    17821 gcaattgcga atgaccggcg tgatcaagcc atcctcgatg ccgactgcaa tgccgatatc
    17881 gatgtgcgca tgccgtctga tggcttggcc agtgaatgac acattgattt ccggatggcg
    17941 ggtcagtgcc agtgctgcgg ctttgatcag gagatcggtg acagagggat ggctgattcg
    18001 gttctgtttg aactgatcac ggaaccgctc tgcctgctcc atgtcgatct cgctcgtcag
    18061 ataaaaatgt ggcacgggtg ctttgctctg tgtcgtcgct cgcgcgatgg ccttccgcat
    18121 ctggctcagg ggctggtccg tgccagccgg cagtatctgc ggcgccgccg cctgcgcttt
    18181 cacgacatcc tcttccacga tgcgcccacc gggaccggtt ccgctgatag tagagaggtt
    18241 gattccacgt tcggcggcca gagcttttgc acggggcgat gctgggacac gagttccctc
    18301 ctggctcctg gatgaggcgg gtgccggacc cgcggcaggc ggcgcgctgg tctgtgctcc
    18361 accactggcc gttggcgcgg cagtgatcct gtcggtcaac gccgaggtga tgtcctcatc
    18421 cgcttcgccg atgacaccga tcaatgtccc ggacatcaca gttgcaccct cctgcacgag
    18481 gatttttcgg agtatgccgg aggcaaaagc ttcgaggtcc atgacggcct tgtccgtttc
    18541 gatttctgcg agcgcctccc ctgcctgaac cgtatcccct tcccgtttct tccacgcaag
    18601 gagcacaccc tcttccatcg tatcggttaa ctttggcatc acgacgcggc tagccataaa
    18661 attccctcac ggatgctgaa aaagtccccc aacttcgttc tcgggtcgac acaatcctca
    18721 acgtagctgt gaagctacgc ctccggttgt gtcatccctg cggcctcgtt ggatggcctt
    18781 tttgatcatc cttaagattt tgaattgtct ttcaatcatc atcactaatt aactactagt
    18841 agagcggtta aaccatgaga agaatttgcc gatcaatcca tctggcgttt cagactgcct
    18901 cacagggtcc ggatactcaa tccacggttc ctgagaaccc aaataggcgc cctttcggaa
    18961 attcagctgg ctgcggagct gcgagtagcc ctgtccttgg agcatacgaa taagttcctc
    19021 gagggcggct cccggtgttg gttgtgtcgt gcttaccacg tgcgtggtct catatcgcag
    19081 aatggcgtga tatcctccgt ccgcttgctc aaacaccacc ggcctgctga agccacgttc
    19141 gcgtgcatcg aagccggcca attgatgttt ctcctctgtg tgctctgaca tgactatcct
    19201 gccagggtgg tctcctattg gtcagagagc ccggaatacg gccaagagat cgctcaatgc
    19261 gatggttttg tttcgcagga tggcacgctg agcttgcatc ggctccatcg acgacgctcg
    19321 ctcatggccg cctctcaaga ttgcggctcc gagggcaagc acgtccttgc attccttttc
    19381 aaccttgctc ttgaccacgg gattcaccgc gaggatgtcc gccatcgtgg ctcgcgctgt
    19441 ggcgagatgc atatgcagtt cctcgtccgg gtatgccttc ctggccgcct cgtcgaggca
    19501 gcgatcgaga tactgcgtca gaaagacata gagccgaacc atcgtctccg tgatctcgat
    19561 atgattgtgt gacgacatgg gtaatcgtgc ctcccgcgtt tatggtgtgc cgcacatgaa
    19621 cttcacaacc tcgatgacgc gtcctttgtt cgggatcgct gcgacttcca atgggcggct
    19681 atagggcatc ggtacatctt cgccagtgac acgttggatg ggggcgtcca ggtagtcgaa
    19741 cgcttccctg tatacactct cggcaatctg cgcgcccaac ccacaaaaat gccagccctc
    19801 ttcaacaatc acaagacggc cggttttttt gaccgaggcc acgatggttg ggatgtccat
    19861 aggcttgagg gtgcggggat cgatgacctc gacctcgatg ccctcgtgac tcagttgatc
    19921 ggccgcctcc agcgccaaca ggagcatctt tgagtaggcg acgattgtca catcgtgtcc
    19981 ggcccgtttc acctcggcaa caccgagagg gatcgtatat tcaccgtctt caacattccc
    20041 ttttgtcgca tacagcaatt gcgcttcgat aaagatgacc ggattgttgt cccggatcgc
    20101 gcttttcagc aggcccttcg catcgtgcgg tgtggacggc gcgatcactt tcaaccccgg
    20161 tacgtgacag aaccatgctt ccagactttg cgaatgctgc gcgcctaact ggtgtgcggc
    20221 actgccgggt ccgcgaatca ccagtggcac cgatagctgg cctccagaca tgtagtgaat
    20281 tttggcggcg ttgttgacga tctgatcgag tgcgagaatg ccaaaattca tggtcatgag
    20341 ctcgacgatg ggatgtaacc
//
LOCUS       DATIPF010000263         1572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_317037, whole genome shotgun sequence.
ACCESSION   DATIPF010000263 DATIPF010000000
VERSION     DATIPF010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317037"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VLL06_06665"
     CDS             <1..705
                     /locus_tag="VLL06_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSS30682.1"
                     /translation="DPITGLPGTNFGLKPQKNLNTEIGTESKLTKDLTIQLVGFLTFF
                     KDEIITQTISGANTASVNADSSQYRGIEASYDWRPLTGLRLSGAYTHIQAMYINFSDR
                     TAAGFLVRDGNKVPNVPTDVVNSKVAYDHAPTGWGGWVEGSYYDSYFLNNSNTFSIPS
                     YVVLNVNIHKNVELSNSWFRFAKFYLELDNITDKKYAASGQVIGGETAAVAAGNQAFF
                     AGYGRAIYGGVTLGMF"
     gene            999..1424
                     /gene="exbB"
                     /locus_tag="VLL06_06670"
     CDS             999..1424
                     /gene="exbB"
                     /locus_tag="VLL06_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HSS30683.1"
                     /translation="MEGLKELVEYGVIGLLLGLSFWAIGVAVERWLFYRQVDVKQFST
                     LDLCEIALTRRLVVIGTVAANAPYIGLLGTVLGIMLTFHTMGTSGALAVTAIMIGLSL
                     ALKATAAGLLVAIPCVVMNNVLRRRVTELLAQYKAQHGG"
BASE COUNT          358 a          412 c          445 g          357 t
ORIGIN      
        1 gatcctatta cgggactgcc cggcacgaac tttggtctca aaccgcaaaa gaacctcaat
       61 acggaaatcg gcacggaatc gaagctcacg aaggacttga ccatccaact cgtcgggttt
      121 ctgacgttct tcaaggacga aatcatcacg cagacgatct caggggcaaa cacggcttcc
      181 gtcaatgcag actcctctca atatcgcggc attgaagcct cgtatgattg gcgaccattg
      241 acgggtctgc gattgtcagg ggcctatacc cacattcaag ccatgtacat taatttttca
      301 gatcgaaccg cagcgggctt cctggtccga gacgggaaca aggtgcccaa tgtgccaacc
      361 gatgtagtga acagcaaagt cgcctatgac catgccccga ccggatgggg cggttgggtc
      421 gaaggcagtt attacgatag ttatttcctg aacaacagca atacgtttag catcccatcc
      481 tatgtggtcc tgaatgtgaa cattcacaag aatgtcgaac tgagcaattc ctggttccga
      541 ttcgccaagt tctacctaga gctggacaac atcacggata agaaatacgc cgcatccggc
      601 caagtgatcg gaggtgaaac cgccgcggtt gctgcgggca accaggcctt tttcgccggt
      661 tacggccgag ccatctatgg tggtgtgacc ttggggatgt tctagctcgt ctgaatatgc
      721 ggagcaggct tgggctggga tgagggcact tcaatggcgc cctcatccat aatcggtatg
      781 cagagctggc ttcatcttct gtggcggtgc ctgtcccgtt gattctccgt cgggggcaag
      841 tgccaaaaaa aagcgccagt ccccagcagt agatcagggt cgaagagcga gccgactggt
      901 agacagctgg tcgtagtaat agatcggcaa tgacagcaga tatgctgata acccatgcgg
      961 gttaggacag tgatgactga aaggactgtg gtgtcttcat ggaaggactt aaggaactcg
     1021 tcgagtacgg cgtcatcggg ctcttgctgg gattgagttt ctgggcaatc ggcgtggcgg
     1081 tggagcgctg gctattctac cggcaggtgg acgtcaaaca gttttccacc ttggatctgt
     1141 gcgagatcgc attgacccgg cgactggtgg tgatcggtac ggtcgccgcc aatgcgccct
     1201 atatcggctt actcggcacg gtcctcggca tcatgctgac atttcacacg atgggcacat
     1261 ccggtgcgtt agccgtgaca gcaatcatga tcggcctgag cctcgcgctc aaggccacgg
     1321 cggcgggact gctggtcgcc attccttgtg tcgtcatgaa caacgtgctg cgacgccgtg
     1381 tgaccgaact tctcgcacag tataaggcac aacatggagg atgacccgaa ttgatctact
     1441 cctgtgcagg ctagacctct gtgctgtggt gtccatgcat agtcgtcgat agacattgct
     1501 tccagtgagg cgtacagaat ccatacgtcg cgccctcttt aagagggtag tctcgacagc
     1561 cacttggtgc tc
//
LOCUS       DATIPF010000264         7106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1644401, whole genome shotgun sequence.
ACCESSION   DATIPF010000264 DATIPF010000000
VERSION     DATIPF010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7106
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644401"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VLL06_06675"
     CDS             <1..262
                     /locus_tag="VLL06_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30684.1"
                     /translation="EGGCKLTTPEHLRAGDFVKVQLWLEGEEAFIDIRLAEVRKIHGH
                     WIAAEVIQVSPNDRMRLNRFIDVPAIGQIEESAFIDHLLIRA"
     gene            complement(311..1126)
                     /locus_tag="VLL06_06680"
     CDS             complement(311..1126)
                     /locus_tag="VLL06_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30685.1"
                     /translation="MLVVHTLSFYSKGSGMRMYWKWVIKSVPILGLTLLMLATTVTPR
                     QAHSNVEAWFNYFGIAEVPLWVATKSIDAWIFWIAFAGVGLWGTHLYVRWNIVKGKLS
                     MVIREGEPWVQVDPGLGHSEVVRTIGQLYTYRVALVNSHDSTLRNVEVKLIALEKKPQ
                     NFSAIGSHLKLRHDRAGATNFNVHPTKDPECMDAMFVDVFRFVSGPGGCSSLRITSLP
                     EESNRPIPMDNYDVKIMATSESGEMAIADMAFIPHPDHIPNFKLLNMQSFPGR"
     gene            1358..1720
                     /locus_tag="VLL06_06685"
     CDS             1358..1720
                     /locus_tag="VLL06_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30686.1"
                     /translation="MLSIPKIISVVLGSFVLCLSLSDASQARHNRGTDPCLDRRAPGI
                     EKCGAGKQQGIRTITGEVLHINGTRLLVKQTDGEEVICQINLSTKIGGDIRPGNRIEA
                     KVNEVEGERLVISIDLAR"
     gene            complement(1779..1913)
                     /locus_tag="VLL06_06690"
     CDS             complement(1779..1913)
                     /locus_tag="VLL06_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30687.1"
                     /translation="MPGSAQLKLTPLNSAAVIGVFVGGLFAIYGFIHDLISSIVDRLK
                     "
     gene            2522..2845
                     /locus_tag="VLL06_06695"
     CDS             2522..2845
                     /locus_tag="VLL06_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30688.1"
                     /translation="MKGLRQILGAISCSVLFCGGLGLSDVASSAENKTSESGKVIIGK
                     LLRFEGNDCIVKNREDGEEMRLYVDKTTKMNVVGVTVGDSVMAKVDDRNHVEVILTDQ
                     KNVFR"
     gene            complement(3003..3092)
                     /locus_tag="VLL06_06700"
     tRNA            complement(3003..3092)
                     /locus_tag="VLL06_06700"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3056..3058),aa:Leu,seq:taa)
     gene            complement(3485..3745)
                     /locus_tag="VLL06_06705"
     CDS             complement(3485..3745)
                     /locus_tag="VLL06_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HSS30689.1"
                     /translation="MSDPGRQVAKAAALVAGGAVIGAGMGLLFAPQAGAETRREVGRY
                     AKKAQVQATRWSRTVQSGVKEVMDRRKAITKKDEQKPLIEAV"
     gene            4092..4907
                     /gene="lgt"
                     /locus_tag="VLL06_06710"
     CDS             4092..4907
                     /gene="lgt"
                     /locus_tag="VLL06_06710"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HSS30690.1"
                     /translation="MTSLNAIPFPNISPVFLELGPLQFRWYGLMYLIGLGGAYFLILR
                     RVKSQGLSMTKDQVYNMIVWAAFGVFIGGRLGYTLFYNFSYYMQHPASILAVWEGGMS
                     FHGGLLGVIVALFWFSQRQGIPAYQVADLAAAATPIGLGFGRIGNFINGELYGRATDV
                     DWCMVFPSGGPACRHPSQLYEAGLEGVFLFILLWTIGKTTPPPGTVFWSFIAGYGLCR
                     MLIEFVREPDAHIGFIFASFSMGQLLSFPMIVVGISMLAFGYQRRALVQAKSQ"
     gene            complement(4901..5266)
                     /locus_tag="VLL06_06715"
     CDS             complement(4901..5266)
                     /locus_tag="VLL06_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HSS30691.1"
                     /translation="MRVSPEEFDVWIQEALAELPARFARLAEEVSIVVEEEPSAEILQ
                     SLELKSEDDLLGLYQGVPINQTSFFQPAGGLPARISIYRGPILRLCRTKAEVIQEVRD
                     TVVHELGHHVGLDDEEMPY"
     gene            complement(5347..6993)
                     /locus_tag="VLL06_06720"
     CDS             complement(5347..6993)
                     /locus_tag="VLL06_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HSS30692.1"
                     /translation="MPGPSAHGAYITDGEKHFYRRQEIIDLVNLLRVIDNPHDTIALV
                     GILRSPLGSMADRDLLALQQNDGLSYQQLERLSNWNHPQAGMIRRLYERLAELHQLAP
                     LRPVSDAIDLIFERLPILELAAASMHGEQAVANLRKIRDMAEALADRPHLTLTGFVDL
                     MMTRVSEQPDEAESALAEESLDAIRVLTIHKAKGLEFPVVILPGLHQGSKSPRKGPSI
                     HHDWSSRCYSLQMGGRSNLGAVLVDKKMAAREEAEQRRLLYVGMTRARDLLVLSGGAT
                     TKPGHDTVLSLLGEVMADEREPSAAAQICIGTSRIMRVITPATIAARRRRAEPLSKVI
                     TTVALGPILMRRQARQVEWEQRRTAPRRVTPTSQAGSRTEAISQRTVVGRDANLARLA
                     GVCAHAVLERWDFSRPRSDISTVIEQACHRYMTQDYLQLLGDVTEDLTAIFDRFLSSE
                     PYKRLQRATVLGREVPFVIPLGRDQVMEGVIDLIYRLDDRIWIADYKTDDVAAEDVQA
                     RADRYRSQADSYSRAVGSALGSPAVSFQFIFLRPGIAIDF"
BASE COUNT         1634 a         1916 c         1900 g         1656 t
ORIGIN      
        1 agaaggtggg tgcaagctca ccactcctga gcacctgaga gcgggggatt ttgtgaaggt
       61 ccaattgtgg ttggaaggcg aggaggcctt tatagacatc cggctggcgg aagtcagaaa
      121 gatccacggg cattggatcg cagcggaagt gatccaggtg agcccgaatg accggatgag
      181 actgaatcga tttatcgatg tccctgcaat tggtcagatc gaagaatctg ctttcatcga
      241 ccatcttctc attcgagcat agctcggcgt ccatcctcac agcagattca catacaccct
      301 cctgtaaaac tcatcgcccg gggaatgatt gcatgttaag taatttgaag ttcgggatgt
      361 gatcagggtg cggaatgaac gccatatcag cgatggccat ctcgccactt tcacttgtcg
      421 ccatgatttt cacatcataa ttatccattg gaatggggcg attactctct tccggcaacg
      481 aagtaatgcg aagggatgaa catccaccag ggcccgaaac gaatctgaat acatcaacaa
      541 acatggcatc catacattcc ggatccttcg ttggatgcac attaaaattt gtggctccgg
      601 cccggtcatg cctcagttta agatgactgc caattgcaga gaaattctgc ggtttcttct
      661 ccaaagcgat gagcttcacc tcgacattcc tcagggtaga atcatggctg ttcaccaatg
      721 ccacacgata ggtgtacagc tgtccaatgg tacgcacaac ctctgaatga cccaggcctg
      781 gatccacctg cacccagggt tccccttcac gaatcaccat ggacaattta cccttcacaa
      841 tattccatcg cacatataag tgcgtacccc atagaccgac acccgcgaac gcaatccaaa
      901 atatccatgc atcgatggac ttcgtcgcga cccatagtgg gacctccgcg atgccgaaat
      961 agttgaacca ggcctctacg ttggaatggg cttgtcgagg ggtgaccgtc gtagccaaca
     1021 tgagcaacgt caatcccaga atcggaactg atttgatcac ccatttccaa tacattcgca
     1081 ttccggaccc cttggaatag aaggagagcg tgtgtacgac gagcatcctt ccatgacacc
     1141 aagatgtacc agaaaatgcc ctgagaatcc tgccgagctg gtacgcctct gtcagtggca
     1201 atgaaaatta cgcgaggacc atagggaatg ggttttgggt catttcacgc ctgcaacctc
     1261 ccttagaaaa gactaggaac atccccagac ttgcaaccct tcggggggtg tataattatc
     1321 ctactcgctc tcttcccctt tcacggagga ggcccccatg ctatccattc caaaaatcat
     1381 cagtgtcgtc ttgggtagtt tcgtgctgtg cctcagtctg tccgatgcca gtcaggcgag
     1441 acacaatagg ggaactgacc catgtcttga ccgcagagcg cctggcatcg agaagtgcgg
     1501 agcaggaaag cagcaaggca tccgcaccat cacgggagaa gtgctgcaca tcaacggcac
     1561 gagacttctc gtcaagcaaa ctgacggcga ggaagtgatt tgccagatca acctcagcac
     1621 caaaattggc ggtgacattc gcccaggaaa tcggattgag gcgaaagtaa atgaagtgga
     1681 aggcgagaga ctcgtcatct caattgactt ggcccgataa agcctaggcc attaactgtg
     1741 cagaggcttg tggggatgtt gcggggttag ctcgttgatt acttgagccg atccacgatg
     1801 gaggagatca ggtcgtgaat aaatccatag atcgcgaaaa gccctccaac aaatactccg
     1861 atcaccgcag cagagttgag cggtgtcaac ttcagttgtg cgctacctgg cacaatcctt
     1921 agacactctc catgatatgg gcatagtcat gagctagccg cttgcggtct cgccatattc
     1981 acgggtatct gtcccatctg gatagaggtt gaactagcgt gagactgagg gacaagtgac
     2041 agttggtagg ttgagtaact gatttgggag agcaccaccc gtccgtccag cgacaatcga
     2101 cccacagcat cgaagacctc cccccaactg aagatcgaaa gggacgtgac cacgtcatcg
     2161 aggcggcacg gtccggttct ttgtaatgta tcaataatag ttgcatctat aggccgcatg
     2221 gcggtgacct ccggttgaaa tgaggtgcgt aagatgggga cgtatcgtga caggagatgg
     2281 gtgagatgcc cactaggaaa tgccctagta ttggtgtacg gagtcgccta cagacatcgc
     2341 catgagcctg gattcgtaaa ggctgaagac ctccagaaaa taattacgag ggtgagactt
     2401 gctgaacagg caggtctgca gtgaaaattt accagggacg ttttaggtca ccatggagga
     2461 tgatagggtg aagagtgcgt agcatatcat catccgtcga cccttaacca aggaggcctt
     2521 tatgaaaggt cttcgccaaa ttctcggtgc tatatcctgt agtgtcctgt tctgtggggg
     2581 tttgggactc tctgatgttg cgtcgtctgc agaaaataag acgagcgaat ccgggaaggt
     2641 tatcatcggg aaattgcttc gattcgaagg aaatgactgt atcgtcaaga acagagaaga
     2701 cggggaagag atgcgcttat atgtggacaa gacgacgaag atgaacgtcg taggggtgac
     2761 tgtcggagat agtgtgatgg cgaaagtaga tgatcggaat catgtagagg taatcctaac
     2821 ggatcagaag aacgtgttcc gttagtattt cacttcagag aatgtgattg cagatctgac
     2881 atgacctgtt tcacgtagtt gaacatttgg gccatatctg ggccatatcg aggcagtcga
     2941 tgccagattt ggccttgtca aaacgctgaa tctctgcaac cggagcgtgg tgtatgtgtt
     3001 gttggtgccg gaggccggaa ttgaaccggc atgcgccttt tgggcacgag ggattttaag
     3061 tcccttgcgt ctgccaattt cgccactccg gcagcggggg ccgcagtggc gggactctag
     3121 cattggggtc taggtcggtc aaggtggacg tcacacgcac tcctaccaca atcggaagtc
     3181 gtcgtatgca acaagcttag ctgcacgtta cgcagcaccc aatgagtggg gtcccgtttg
     3241 gtcggtacgc tcctcattca aggatcttca gtgcctttgc ccactcgtag gggccagcct
     3301 ccttctcgat tccgcacaaa gggggcatat cccttgcaga actcctggca ggatgctgct
     3361 ccgcatcagg gccccgcatt cgtctcgctg gggaactcca caatttcgtg acggaccgtt
     3421 atcaacaatg aggcccggtc aggcgcttgt acctccgatc tgatcgctaa gcgagccctt
     3481 caaactacac agcttcgatg agcggctttt gctcgtcctt cttggtgata gccttacggc
     3541 gatccatcac ttctttcaca ccggactgaa cggtccggct ccagcgagtt gcttgcactt
     3601 gagccttttt agcatagcga ccgacctcac gccgggtttc tgcaccggct tgtggcgcga
     3661 agagcaatcc cattcccgct ccgatcaccg ccccacctgc caccaacgct gcagccttcg
     3721 ccacctgtcg tcctggatct gacatgataa acctccttgg tacggttgga tgagaaatgt
     3781 gattgccgac atcctgcacg cacatgtctt actctctcta tcagcagtaa gcgtgccaga
     3841 ggaaaaacca tggaaatcgc ccatgattcg tcattgcact gttcctacga ttaagtgtca
     3901 ttaattttca gttgtgtttt cctcacaggt cacgacacag gcgtgacaga aaaccaacat
     3961 acgaaagtag taggccgctc gaactggctc tgagcgatga tcagagtcga tttctcgctg
     4021 ggcgttagcg tgcttgcaat ggttgagacc ctcccctatg attctccgac gctcgcccac
     4081 atgaggaact catgacttcg ttgaacgcaa tcccgttccc caacatcagt cctgtgttcc
     4141 ttgaacttgg ccccctacaa tttcgctggt atggtctgat gtatctaatc ggactggggg
     4201 gcgcctactt cctgatcttg cgccgagtga agtcacaagg cctctccatg accaaggacc
     4261 aggtctacaa tatgattgtc tgggcagcct tcggagtgtt tatcggagga cgtctcggct
     4321 acacactctt ctataatttt tcctactata tgcagcatcc ggccagtatt ctggcagtct
     4381 gggaaggagg catgtccttc catggcgggc tgctcggcgt catcgtcgca ctattttggt
     4441 ttagccaacg gcaaggcatt cctgcctacc aggtcgcaga cctggcagcc gctgccacgc
     4501 cgatcgggtt gggatttgga cggatcggta attttatcaa tggggagttg tatgggcgag
     4561 cgaccgacgt cgattggtgt atggtatttc cgagcggggg gcctgcctgc cgccatcctt
     4621 cacaactcta tgaagcaggg cttgagggtg tattcttatt catcctgtta tggaccattg
     4681 ggaaaaccac accaccaccc ggtacagtct tttggagttt tatagccggc tatggcctgt
     4741 gccggatgtt aattgagttt gttcgcgaac cggatgctca cattggtttt atctttgcct
     4801 ccttctccat gggccaactc ctttccttcc ccatgatcgt cgtagggatc agcatgctcg
     4861 ccttcggcta ccaacgacgg gctttagtcc aggcgaagtc tcagtaaggc atttcttcat
     4921 catcaagccc gacatggtgt ccaagttcat ggaccaccgt gtcacgaacc tcctgaatga
     4981 cttctgcctt tgttctgcag agccgcagga tagggcctcg atagatggag atacgggccg
     5041 gcagcccgcc ggcaggttgg aagaacgatg tctgattgat cgggacgcct tgatagagcc
     5101 ccaacagatc gtcttctgat ttcaattcaa gactctgcaa gatttcagcc gagggttctt
     5161 cttcgaccac gatagagacc tcttcggcga gcctcgcgaa gcgggccggc agttcggcga
     5221 gtgcttcttg gatccagaca tcgaattctt ctggtgagac tctcattgga cgttcatctg
     5281 gtttgtctgg tttgtctggt tagtttcgtt caaccaaaca aacgagacaa accaaataac
     5341 ggcctgctaa aaatcaatgg cgatacctgg cctcaaaaag ataaactgaa acgaaacagc
     5401 cggcgatcct aacgcactcc cgacggctcg cgaataacta tccgcttggg atcggtagcg
     5461 atcggcccta gcctgaacgt cctcggccgc cacatcatcc gtcttatagt cggcaatcca
     5521 gatccggtcg tcaagccggt atatcaggtc gatcaccccc tccatcacct ggtccctccc
     5581 cagggggatg acgaacggaa cttcccgccc gagcaccgtt gcacgttgca gtcttttgta
     5641 cggctcggag gataggaacc tgtcaaagat cgccgtgaga tcctcagtga cgtcacccag
     5701 caattgaagg taatcctgag tcatatagcg gtgacaggcc tgttcaatga cggtactgat
     5761 atcagaacgc ggcctactga agtcccaccg ttcgagcacc gcatgggcac agactcccgc
     5821 gagtcgagcc agattggcgt cacgccctac cacggtgcgc tgggagatcg cctcggtcct
     5881 gcttccggcc tggctcgtag gagtcactcg ccggggcgcc gttcgacgct gttcccactc
     5941 gacctgtcga gcctggcggc gcatgagaat ggggccaagg gcgacagtag taatgacctt
     6001 cgacagaggc tcggcgcggc gccgtctcgc ggctatcgtg gcaggcgtga tgacgcgcat
     6061 aatccggctg gttccaatgc agatctgggc agccgccgag ggctcccgct cgtcggccat
     6121 cacctctccg agcagagaga ggacggtgtc atggcccggt ttggtggtcg ccccgccgga
     6181 taggaccaaa agatcgcgtg cccgtgtcat cccgacatag aggagccggc gctgctccgc
     6241 ctcttcccgc gctgccatct tcttgtccac gagcaccgct ccaagattgg agcgaccacc
     6301 catctgcagg ctataacaac ggctcgacca atcatgatga atggacggac ccttccgcgg
     6361 actctttgac ccctgatgca gacctggaag aatgaccacg ggaaactcca atcctttggc
     6421 cttatggatg gtcaacacgc gaatggcatc caatgattcc tcagccagtg cgctttctgc
     6481 ctcatccggc tgctccgaca ctctggtcat catcaggtca acgaatcctg tcaaggtaag
     6541 atgggggcga tcggcaagcg cttcagccat atcccggatc ttccgaaggt tcgcaacggc
     6601 ctgctcgcca tgcatcgatg ccgctgccaa ctcgagtatg ggtagtcgtt cgaagattag
     6661 atcgatggcg tctgatacgg gtcgtaatgg agcgagttgg tgcaattcgg ccaatcgttc
     6721 atagagcctt cttatcatcc ctgcctgtgg gtggttccag ttggacaacc gttccagttg
     6781 ctggtagctc aacccatcat tctgctggag ggccagtaag tcccggtctg ccatgctacc
     6841 gaggggtgag cgtaagattc ccacgagcgc aatcgtatcg tgagggttgt cgatgactcg
     6901 gaggaggttg accagatcaa tgatttcctg ccgccgataa aaatgcttct ctccgtcagt
     6961 gatataggca ccgtgggctg atggccccgg aatccgacat cgatctgctg ttcatcctgc
     7021 cttacaaaca gacggcgtgg ggcgagcagg tcgccgaagc gatcctgtat tgtctgtggg
     7081 atatgggcct caaggtcggc cacgcg
//
LOCUS       DATIPF010000265         1995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_756977, whole genome shotgun sequence.
ACCESSION   DATIPF010000265 DATIPF010000000
VERSION     DATIPF010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756977"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VLL06_06725"
     CDS             <1..503
                     /locus_tag="VLL06_06725"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HSS30693.1"
                     /translation="NEAKLKDIAYPPLFVPSGKTADDLMKQFQQEKRHMAVVVNEFGG
                     VMGIVTLEDLLEEVVGEIMDETDITEELIKRLGKNHILVHSRTEVRKVNDFLKVDLGD
                     EAVTIGGLIQQELGRIPKVGEEVRIANCRIVIHEAEPRSIRSVQIFREEKVPAPVEAP
                     NLDLVR"
     gene            complement(496..>1995)
                     /gene="ligA"
                     /locus_tag="VLL06_06730"
     CDS             complement(496..>1995)
                     /gene="ligA"
                     /locus_tag="VLL06_06730"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HSS30694.1"
                     /translation="YMRLDDFQALNRRITERGDDAFANPRNAAAGSLRQLDSTITATR
                     PLVMTCYEIMVISTPIPSTHWDELERLAQWGLPVPTHRRLCASIEEVMEFHHETESMR
                     DQLPYEIDGVVVKVNRRDWQSRLGMKSRSPRWAIAYKFLPRKEITVVQDIVVSVGRTG
                     TLTPVALLKPVEVGGVTISRATLHNADEVMRKDIRIGDTVKVERAGDVIPAIAERVPI
                     DGEQRSAPFGMPDHCPVCGSKVGREGAYFYCTGQLVCGAQLKGAIEHFSSKHALNIEG
                     LGKKTVAQLVDEGLVRSLADLYHVTKANLVRLEGFADRSATLLLESIASSKTVSLDRF
                     LMGLGIRQVGQHIAKVLAREFGSLDDIMSADRERFQQIREIGPEISESLTVYWSEPHN
                     REVIAQLRESGVQVAPGLATGGRRNLPLAGKTFVFTGGLDHFSRDGAQQAVEAAGARV
                     SSSVSKKTSYLVVGRDPGSKLDQARALEVTILTEQEFAALIGKDEKDLT"
BASE COUNT          458 a          567 c          550 g          420 t
ORIGIN      
        1 aaaatgaggc aaaactcaag gatattgcct atcccccgct gtttgtgcca tcaggtaaaa
       61 cggcggacga cctcatgaag cagtttcagc aggagaaacg gcacatggcc gttgtggtca
      121 atgagttcgg cggggtgatg gggattgtga cacttgaaga tcttctcgaa gaagtggtgg
      181 gggagatcat ggatgaaacc gatattacgg aagagctgat caaacgctta gggaagaacc
      241 acatccttgt tcacagtaga accgaagtgc gcaaggtcaa tgatttcctg aaggtcgatc
      301 ttggcgacga agccgtgaca atcggcggcc tgattcagca ggagcttggc cgtattccca
      361 aggtcggaga agaagtgcgg atcgccaact gccggatcgt gatccatgaa gcggaacctc
      421 gatccatccg tagtgtccag atattccgtg aagagaaagt accagccccc gtcgaagctc
      481 cgaacctcga cctcgtcagg taaggtcctt ctcgtctttc ccaatcagcg cagcaaactc
      541 ctgctccgtc aaaatcgtca cctccagggc ccgggcttgg tccagtttgg agccagggtc
      601 tcgtcccacc acaagatagg acgtcttttt actcacgctc gaagaaactc gcgctcctgc
      661 ggcttccaca gcctgttgag cgccgtcccg cgagaagtga tccagcccgc cggtaaacac
      721 aaacgtcttt cctgcgagcg ggaggtttct ccggcctcct gttgccaggc cgggtgcaac
      781 ctgtacgccg gattcccgga gctgtgcgat gacctcccgg ttgtgaggct cagaccaata
      841 gaccgtcaaa ctttcggaaa tctcaggacc aatttcacga atctgttgaa accgttctcg
      901 atccgccgac atgatgtcat cgagagaccc aaactcacga gccaggactt tggcgatatg
      961 ttggccgact tgccggatcc cgagtcccat gagaaagcga tccagagaga cggtcttact
     1021 gctggcaatc gattccaaga gaagcgtggc ggaccgatcg gcaaaccctt caagccgaac
     1081 gagattggcc ttggttacgt gatagaggtc ggcgagtgag cgtaccaatc cctcatcgac
     1141 cagctgcgcc acggtcttct tccccaggcc ttcgatgttg agtgcatgct tggaggaaaa
     1201 atgttcgatc gcccccttca attgagcgcc acagaccaat tgaccggtgc agtagaaata
     1261 ggcaccttcg cgaccgacct ttgacccgca gaccgggcag tgatcaggca tgccaaaggg
     1321 ggcgctgcgt tgttcaccat cgatcgggac acgctcggca attgcgggaa tcacatcgcc
     1381 ggcccgctcg accttgacgg tatcgccgat ccgaatgtct ttccgcatca cctcatcggc
     1441 attatgcaac gtggctcggc tgatggtcac gccgccaacc tccacgggtt tcaacagcgc
     1501 gacgggcgtc agggttccgg ttctcccgac ggagacgacg atatcctgca cgacggtgat
     1561 ttccttgcga ggaagaaatt tataagcgat tgcccagcgg ggactacgag acttcatgcc
     1621 aagacggctc tgccagtctc ggcgattcac ctttaccacc acaccatcga tttcataggg
     1681 cagctgatcc cgcatcgact ccgtttcgtg atgaaactcc atgacttctt cgattgacgc
     1741 gcagagtcgc cgatgcgtcg gcacagggag accccactgg gccaaccgct ccagttcatc
     1801 ccaatgtgtg gatgggatgg gtgtagagat aaccatgatt tcgtagcagg tcataaccaa
     1861 tggtcgggtc gcggtaatcg tagaatccag ttgtcgaagc gaacccgcgg ctgcattacg
     1921 tggattggca aacgcgtcgt cgccccgttc cgtgatgcgc cgattcagcg cttggaaatc
     1981 gtcgagtcgc atata
//
LOCUS       DATIPF010000266         3074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_317360, whole genome shotgun sequence.
ACCESSION   DATIPF010000266 DATIPF010000000
VERSION     DATIPF010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317360"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="VLL06_06735"
     CDS             complement(<1..1258)
                     /locus_tag="VLL06_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30695.1"
                     /translation="MPSDPRRAVSTLSAHLRFSTNRSFAQDLEQEGGRVAHLSTGHVV
                     FYRPDGRRFLATDPDGNPLHECEWGSDATGGAVLLRARIRLDWGQWIGLRPTGLVNET
                     SLNLASTPGWQRLTTDDLRMMAAQALRVPIEDVRFFYRDDDLQIGPTGRATIRQRKDA
                     LYVLRDGDFEQASFMSCMGAMSWSAIDFLPVVELFQSLLPGTGSAVFELIRGLYDDQN
                     EGQPNPRPLRYRGIPTYPSKAAFRLFSSFFTPQASGGDNPFALFMNPERANHVTWLPA
                     EMPPIRYFDQRTEVCLTFHGSCLHKVTLASDSSGLSYMSSKGRRCGPYDRSAEIVGTH
                     VILKDRDRETTLAVPILQGKQEPSRERLAMSPVDWRSVFAQGVPVIPPADAYGAVLLY
                     PDDESEIGECAAQPFVADYVQDLTEQD"
     gene            complement(1259..2593)
                     /locus_tag="VLL06_06740"
     CDS             complement(1259..2593)
                     /locus_tag="VLL06_06740"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HSS30696.1"
                     /translation="MLSDKNNQAEASIKPIESAGVEVVAPIPIPEYVNEMVMRAKSAS
                     GRLATLSTAVKNQALLAMAEALEEQKEALLAANELDLEAFVATPEKKAMRDRLRLTTE
                     RIVEMAAGLREVAALADPLGDMPKMWTRPNGMQVGRVRVPIGVIGIIYESRPNVTADS
                     AGLCLKSGNACVLRGGSEAIHSNAAIAAVLSEAAEKAGVPAGAISFVARPDRELVPLL
                     LKQDRYIDLIIPRGGESLMRVVTEHATIPVMKHDAGVCHIYVDADADPAMAERICFNA
                     KVQRPSTCNAMETLLVHQGIARQWLAPFIEKLVAAKVEVRGCQKTCELSSSAKPASDE
                     DFGKEFLDLTVAVKVVKNVDEAMEHIAKYGSQHTEAIVTSDYPKAMRFLREVDASAVL
                     VNASTRLNDGYQFGLGAEIGISTSRIHARGPMGLEELTCFKFIVLGSGQIRE"
     gene            complement(2769..>3074)
                     /locus_tag="VLL06_06745"
     CDS             complement(2769..>3074)
                     /locus_tag="VLL06_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30697.1"
                     /translation="PMGHGEWEYLRAPTGGIMGVRSKSLGKPFRQNEFPPAVRHFEGR
                     TSYRDWVFQHPNPSGNSIVPPGTMPSSVTPSMTGSSTPPGGIPPVAPPVQGQPAMNP"
BASE COUNT          655 a          915 c          858 g          646 t
ORIGIN      
        1 aatcctgttc ggtaagatcc tgcacgtaat cggccacaaa gggttgtgcc gcgcactcac
       61 cgatttcaga ctcgtcatcc ggatagagca gcaccgcgcc gtaggcgtcg gcaggtggga
      121 tgaccggaac cccctgggca aagaccgacc gccagtcgac tgggctcatg gccagtcgct
      181 cgcgtgatgg ttcctgtttc ccttgcagaa tgggcacggc caacgtcgtc tcacgatctc
      241 gatccttcag gatgacatgc gttcccacaa tctctgcact ccgatcgtac gggccacatc
      301 gacggccttt cgagctcata taggagagcc cggaggaatc tgacgccagc gtcaccttgt
      361 gcaagcagct gccgtggaag gtgaggcaca cctccgtgcg ctgatcgaaa tatcgaatcg
      421 gcggcatctc tgccggcagc catgtgacgt gattggcccg ttcagggttc atgaagagcg
      481 cgaacgggtt atcgccacca gacgcctgcg gggtaaagaa gctactaaat aagcgaaagg
      541 ccgctttcga tggataggtg ggaatgcctc ggtaccgcaa gggtcgggga ttcggttgtc
      601 cctcattctg atcgtcgtaa agtcctcgaa tcaattcaaa gactgccgac cccgttcccg
      661 gcaacaacga ctgaaagagt tcgacgacgg ggagaaaatc gatcgccgac caactcatcg
      721 ctcccataca ggacatgaaa ctggcctgct cgaagtcccc atccctcagc acatacagag
      781 catcttttcg ctgacgtatg gtcgcccgac cggtcggccc gatctgtaga tcgtcatcgc
      841 gatagaaaaa tcgcacgtcc tcgatcggga cccggagcgc ctgagcagcc atcatgcgca
      901 aatcgtcggt tgtgagccgc tgccaaccag gtgtgctggc cagattgagg ctggtttcat
      961 tcaccaatcc agtcggcctc aatccgatcc attgccccca atctagacgg atgcgagcac
     1021 ggaggaggac tgcgccaccc gtagcgtcag acccccactc acattcgtgc aacgggtttc
     1081 cgtctggatc ggtcgccaga aaccggcgac cgtccgggcg atagaagacg acatgcccag
     1141 tggataggtg ggcgacccgc ccgccctcct gttcgagatc ctgtgcgaag gaacgattag
     1201 ttgaaaatcg gagatgagca gaaagtgtcg atacagcacg acgtggatca gagggcatct
     1261 actcgcggat ttgcccgctc cccaacacaa taaacttgaa gcaggtcaat tcttccagcc
     1321 ccatcgggcc tcgggcatgg atacgggatg tgctgatacc gatttcggct cccaatccga
     1381 attgatagcc atcgttcaac ctggtcgacg cgttcaccag cacggcgctg gcgtcaacct
     1441 cacgcagaaa ccgcatggcc tttggatagt cggatgtcac gatggcttct gtatgctgtg
     1501 agccatactt ggcaatgtgc tccatcgcct catcgacgtt cttgaccact ttcaccgcca
     1561 cggtgagatc gaggaattcc tttccaaaat cttcgtcgct cgccggcttg gccgatgacg
     1621 acagttcaca ggttttctgg cagccgcgga cctcgacctt ggccgccacc aacttctcga
     1681 tgaagggcgc aagccactga cgcgcaatgc cctgatggac taagagggtc tccatcgcat
     1741 tgcaggtcga cgggcgctgc acctttgcgt tgaagcagat ccgctccgcc atggccggat
     1801 cggcatcggc atcgacatag atgtgacaga caccggcgtc gtgcttcatc acagggatcg
     1861 tggcgtgctc ggtgacgacg cgcatcagcg attcgccgcc tcgcgggatg atcagatcga
     1921 tatagcgatc ttgcttcagg agaagaggaa ccaattcacg gtcaggtcgc gcgacaaaac
     1981 tgattgcccc agccgggaca ccggctttct ctgccgcttc cgagaggacc gccgcaatcg
     2041 ccgcattcga atggatggcc tcactccctc cccgcaacac gcaggcattc ccagacttca
     2101 aacagaggcc agcagaatcc gccgtcacgt tcgggcggga ctcataaata atgccaatga
     2161 cgccgatcgg aacccgcaca cgccccacct gcataccgtt cggccttgtc cacatcttcg
     2221 gcatatcccc gagtggatca gcgagcgcag ccacctcgcg taagcctgcc gccatctcga
     2281 caattctctc ggtcgtcaga cgcaaccgat ccctcattgc cttcttctcc ggcgtagcta
     2341 cgaatgcctc aagatccagt tcgttcgcgg cgagcagcgc ttccttctgt tcttcgagcg
     2401 cctctgccat tgcgagaagg gcctgattct tcactgctgt cgaaagcgta gccagtcggc
     2461 ccgaggcact tttggctcgc atgaccattt cgttgacata ctctgggatc gggatgggcg
     2521 cgaccacttc cacgcctgcc gattcgatgg gtttgatcga cgcttccgct tggttatttt
     2581 tgtcagacag cataggaaag acgcctactt tagggcctcg gattactgac aggatacagt
     2641 gggagtttct acgcggtcaa ggcgggggga ggattggttt ggttaatctg gattgttgtg
     2701 ttcatctggt agttgagtgc aaccaaacaa gccaaataga tcaaagtcga tgcggacacc
     2761 ggcaggggtt acggattcat ggccggctgt ccttgaaccg gcggtgcgac tggagggatt
     2821 ccaccaggag gggtcgatga tccagtcatt gatggcgtca cagaacttgg catagtccct
     2881 ggtggcacga tcgagtttcc tgaggggttc gggtgttgga agacccaatc acgataggag
     2941 gttcgtcctt cgaaatggcg gacggcagga ggaaattcgt tctgccggaa tggcttcccg
     3001 aggcttttgc ttcggacccc catgatgcca cctgtcggcg cccgaagata ttcccattct
     3061 ccatgcccca ttgg
//
LOCUS       DATIPF010000267         3845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_582615, whole genome shotgun sequence.
ACCESSION   DATIPF010000267 DATIPF010000000
VERSION     DATIPF010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582615"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1700)
                     /locus_tag="VLL06_06750"
     CDS             complement(<1..1700)
                     /locus_tag="VLL06_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HSS30698.1"
                     /translation="MSDGLIFADSQDRFLAETTWDRNVVVVAGAGTGKTTILVNRILN
                     LLMREPNPLAITEIVALTFTNKAATEMKQRLRTQLLHLTEQTDDTIAVLCARYRLSTE
                     QVSERAKMALEQMEKAQIGTLHSFAAHLLRLHPMEAEIDPLFQEDDGSRFKELFHSDW
                     DRWLDDELGLAGPQHDRWRRVLAGANLDDLQQFATALSGDLVDLDELERQCRSRALEG
                     ALRDWVVAMQGRAATLLAEQDRPKRRKAEQMLAAAVQLLALLLEQGLRGLDQVTQDER
                     LVLHKDLGKAPAGWEEAAFQEAASIIGLAQDFLTVDQVYFQEVVTLVRPFLDHVRHGF
                     HNSGWIAFDGLLARAKILLRDHPAVRARIKQAYRAILVDEFQDTDPVQYEIILYLAER
                     LGSHQIAWQDVDLEPGKLFIVGDPKQSIYAFRRADIEAFERVVAKIRAGGGGVYSLVT
                     NFRSDGTVLDVVNNVFDRLFQPVDHVQPTNERLAARPQRKPEVSVSGAQLRLVTPGED
                     DEEFDVQAATRAEAEALARWLKEELLADTTVTDRDRRQGPLQPGHIALIFRKLTQAQV
                     "
     gene            complement(1693..>3845)
                     /locus_tag="VLL06_06755"
     CDS             complement(1693..>3845)
                     /locus_tag="VLL06_06755"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HSS30699.1"
                     /translation="LVKTLLRLASLPLNDFDRAAMLDVVTSPFYGQGPGSGSANLRPD
                     LWRSLVYTLGIAKGEAEWRRLAVPASAAILRHAGAEFDEDDQTMVGTSETSQLTYLWD
                     IVSGLIHDCRALPAQGSIGTLTDAFLTLVKSHMQVPALFDGSSADLTDPTGLTRVASL
                     IRVSLARLQQLDSLGGDLSWEEWIELFRRVLDETSIPIEADSHQGVQVLDAMTARGRT
                     VRALFVLGMNEKLFPRYVREDPFLRDRQRVVLESTLGYKIDEKLAGHEEELLLFELLS
                     RSVSHRLYLSYQRADETGRVMVPSSFIAMALRDSRFIGKPEVTVPRRLTQRISEQPAI
                     QDLLPAEELALGCLLQGHDARPVFNAMGRDQALFEQGLAALRIIERESPELGPFDGMV
                     GAPALASSGATERSFSPTALERYATCPFQYFAEKVLRVKPVQRLGHDHLPPLMLGTLL
                     HESLRVSYERLVLLQWPDDQLTEARVQSTVNEAVTDTFAAHAASQGTGHALLWTLAHE
                     QVTGLVAAAVFSDQAEYGSTGFRPLAFEAAAQGSVLLESETSSVSLKIHGTVDRVDFR
                     AEPPAIRIVDYKFKQGTEITAVDRNLALSAVRGFRLQPPLYARMTVSSLPAPTDVHLL
                     FLAPQWKQPISRSTFDAGLWTGHTGDMIGQTLSTLIQGIARREFFILPDGYCDYCEFS
                     GACRRHDAMAWWRSYRSPQARVLRRLRRQKVHDE"
BASE COUNT          848 a         1131 c         1091 g          775 t
ORIGIN      
        1 tagacttgtg cctgagtcaa ctttctgaag atcagggcaa tatggcctgg ttggagtgga
       61 ccttggcgtc gatcgcgatc agtgaccgtc gtgtcggcga gaagttcctc cttcagccag
      121 cgagccaacg cctccgcctc cgcccgagtc gcagcctgga catcgaactc ctcgtcgtct
      181 tctcccggcg tgacgaggcg aagctgtgca ccggacactg agacttccgg cttgcgctga
      241 ggcctggctg ccaaccgttc gttggttggc tgaacatggt ctacgggttg gaaaagccgg
      301 tcaaagacat tgttgacaac gtcgagcacg gtcccgtcgc tgcgaaagtt cgtcaccaga
      361 gaatagacac ctcccccacc cgcgcgaatc tttgcgacca ctcgttcgaa agcttcgata
      421 tcggcccggc ggaaggcata gatcgattgc ttgggatctc ccacgataaa taatttcccc
      481 ggctctaagt ccacgtcctg ccaagcgatc tgatggctac ccagccgttc agcaaggtag
      541 agaataatct catactgcac cgggtcggtg tcctgaaact catcgaccag gatagctcga
      601 taggcctgtt tgatccgcgc gcggacggca ggatggtcgc gcaacagtat tttagcccgg
      661 gccaataacc cgtcgaatgc gatccacccg gagttgtgaa agccatgacg aacgtgatcg
      721 agaaacggtc gtacgagcgt caccacctct tgaaaataca cttggtcaac agtgaggaaa
      781 tcttgggcca gtccaataat ggaggctgct tcctggaacg cagcttcttc ccaaccagcg
      841 ggggcctttc ccagatcttt gtgcaggaca agccgttcat cctgagtgac ctgatcgagc
      901 ccccggagcc cttgctccaa cagcaacgcc agtaactgca ctgcggccgc aagcatctgt
      961 tccgccttac gccgtttcgg ccgatcctgt tcagcaagga gggtggcagc acggccctgc
     1021 atggcgacaa cccagtcgcg caaggcgccc tccagggcac gggatcgaca ctgacgttcc
     1081 agctcatcca gatctacaag gtccccgctg agggcagtgg caaactgctg gagatcgtcg
     1141 agattggccc cggccagaac tcgtcgccag cgatcatgct gtggtccggc caatccgagt
     1201 tcatcatcca gccaacggtc ccaatctgag tgaaacaatt ccttgaagcg tgatccgtca
     1261 tcctcttgaa agagtggatc aatttctgcc tccataggat gaagtctcaa gaggtgcgca
     1321 gcaaaactat gcaatgtccc aatctgggcc ttttccatct gctcaagtgc catcttggcc
     1381 cgttcgctta cctgttcagt ggaaagacga tagcgggcac acaagaccgc gatcgtgtca
     1441 tcggtctgct cggtcagatg aaggagttgc gtacgaagcc gctgcttcat ttcggtcgcg
     1501 gctttgttgg tgaaggtgag ggccacgatc tccgtgatgg ccaaggggtt cggctctctc
     1561 atgaggaggt taagaatgcg attgaccagg atcgtggtct tgccggtccc ggcacccgca
     1621 accaccacga cattgcggtc ccaggttgtc tccgccagga accgatcctg ggaatccgca
     1681 aatatcaatc catcactcat cgtgtacttt ctgccttctt agccggcgta acactcgtgc
     1741 ctggggagac cggtatgagc gccaccaggc catggcatcg tggcgccggc acgcaccaga
     1801 gaattcacag tagtcgcaat agccgtcggg aagaataaaa aactctctcc tcgcaatgcc
     1861 ttggatcaac gtggagagtg tctgtccaat catgtcaccg gtatggccgg tccagagacc
     1921 tgcgtcaaac gttgagcgag agatcggctg cttccactgg ggagccagga agagcaagtg
     1981 gacatcggtt ggtgcgggca gcgaagagac cgtcatgcgg gcatagagag gaggttgcag
     2041 tcgaaatcct cgcaccgccg acagtgcgag gttgcgatcc acggcggtga tctcagtccc
     2101 ctgcttaaac ttatagtcca cgatacggat tgccggaggc tcagcccgaa aatcgacacg
     2161 atccaccgtg ccatgaatct tgagggaaac cgaggacgtc tcggattcca gcaggacgct
     2221 gccctgggcg gcagcctcaa acgcaagagg gcggaatcca gtcgacccat actccgcctg
     2281 gtcggaaaag acggcggccg caaccaatcc agtcacctgt tcgtgggcca atgtccagag
     2341 taacgcatgc cccgtcccct ggcttgcagc atgagcggca aacgtatccg tcactgcttc
     2401 attcaccgtg gattgcaccc tggcctcagt cagctggtca tccggccact ggagcagcac
     2461 caatcgctcg tagcttactc gaagtgattc atgaagaagg gtccccagca tgaggggagg
     2521 taggtggtcg tgaccgagcc gttgcacagg cttcactcga agaacttttt ctgcgaaata
     2581 ctgaaaggga caggtcgcat accgttccaa agcggtgggc gaaaaactcc gctccgtcgc
     2641 ccccgatgac gcaagtgccg gtgcgccgac cataccgtca aaggggccaa gctcggggga
     2701 ttctcgctcg atgattctca gtgcggccag gccctgttca aaaagggcct ggtcccgtcc
     2761 catcgcatta aatacgggtc gtgcatcgtg gccctgcaac agacagccca aggccaattc
     2821 ctcggccggt aatagatctt ggattgccgg ctgttcactg atccgctggg tcagccgtcg
     2881 agggaccgtc acttccggct ttccgatgaa tcgcgaatcg cgcagggcca tggcgataaa
     2941 gctggacggg accatgacac gtcccgtttc gtcggctcgc tgataggaga gatacaacct
     3001 atgacttacc gagcggctga ggagttcgaa taggagcaat tcctcttcat gcccggcaag
     3061 tttctcatcg atcttgtagc ccagggtcga ttccaggacg actcgttgac ggtcccgtag
     3121 aaatgggtct tcacggacgt accggggaaa gagtttttcg ttcatgccaa gaacaaagag
     3181 cgcccgaacc gttctgccgc gggccgtcat ggcgtccagc acctgaacac cctggtgact
     3241 gtccgcttcg atgggaatgc tggtctcgtc caagacccgc cggaacaatt caatccattc
     3301 ctcccaggag agatctcccc cgagcgagtc cagttgctgt aggcgggcca acgatactcg
     3361 gataagggag gcaaccctgg tgagcccggt cgggtctgtc aggtccgccg aagatccatc
     3421 aaacaaggca ggcacctgca tatgggattt caccagtgtg agaaatgcgt ctgtcagtgt
     3481 gcctatcgat ccttgcgcag gaagagcgcg gcaatcgtga atcagtcccg aaacgatgtc
     3541 ccacaagtag gtcagctgag acgtctcgga cgtcccgacc attgtctggt catcttcgtc
     3601 aaactccgct cctgcatggc gaagaattgc cgcactggcc ggcactgcca ggcgcctcca
     3661 ttccgcttct cctttagcga tacccagggt gtagacgaga gagcgccaga gatcaggcct
     3721 gagattcgcg ctcccggaac cgggtccctg gccatagaaa ggcgacgtga ccacatccag
     3781 catggccgca cggtcaaagt cattcagtgg gagcgaggca aggcgcaaga gggttttgac
     3841 cagag
//
LOCUS       DATIPF010000268         5236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1644586, whole genome shotgun sequence.
ACCESSION   DATIPF010000268 DATIPF010000000
VERSION     DATIPF010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644586"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VLL06_06760"
     CDS             complement(<1..735)
                     /locus_tag="VLL06_06760"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HSS30700.1"
                     /translation="MSRTISEDEDKRQHLLHHLDERVKELTALHGVVRLLQDSSVPLN
                     EVLHHVVLLLPPAWQYPDIAVACIRIDGVVAMTPRFRETPWMQRAALTTSAGRQGSLE
                     VGYLEEKPQEHEGPFLVEERNLINSLADSLSSYLTRREAEEGLRDTHAQLQALSRQLM
                     YLQEQERRQLAHDLHDEVGQALTALKMNLQTMQRVADMSAVQNSLLDSFGILDTLLAR
                     VRDLSLDLRPSLLDHLGVVPAIRWYAI"
     gene            complement(1061..1432)
                     /locus_tag="VLL06_06765"
     CDS             complement(1061..1432)
                     /locus_tag="VLL06_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30701.1"
                     /translation="MRSRNRIIRALAMLPLVGLLSLTGNNGAMAASLEEKLAATGTAE
                     DHLAAARLYQNEARDLDAEAAKFETAVSKIGRYDDTKGFKRAALTAAAQEKRVDAKEM
                     QELYAAHLRQANALHGKVQPQ"
     gene            complement(1727..2125)
                     /locus_tag="VLL06_06770"
     CDS             complement(1727..2125)
                     /locus_tag="VLL06_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS30702.1"
                     /translation="MPRILVIDDEQLLRSTVVTILTRAGFSVEDASDGQAGIAMFHKN
                     PPDIVLTDIFMPNRDGIEIIKELKQSNPQTKIIAMTGGGHLRMMEIASAAKVLGADHV
                     LDKPFDSESLLAAINAVLGGYPPAKNQLGA"
     gene            complement(2159..2575)
                     /locus_tag="VLL06_06775"
     CDS             complement(2159..2575)
                     /locus_tag="VLL06_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS30703.1"
                     /translation="MLPYKVLIVDDEDSIRTLVQTMLKQESRHVALAAKGKDAITVFK
                     MERPHLTILDIDMPDIDGLTVLREIRTIDPQAKVIIFTGGDSPSVEREARALGVTDFL
                     HKGFALPPISELQSRGSTSTVLEAPSSTSPVLRPKV"
     gene            complement(2656..3030)
                     /locus_tag="VLL06_06780"
     CDS             complement(2656..3030)
                     /locus_tag="VLL06_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS30704.1"
                     /translation="MDKKKILIVDDNADTRLLLSARLKAHQYHTVFAADALQAMSVAQ
                     KERPDAMLLDLGLPGGNGMVVLQRFKANTSLNGIPIIIVTAEDPLVAEAGTIEAGAVA
                     FLQKPVDQDKLLAAVQQAIGTT"
     gene            complement(3049..>5236)
                     /locus_tag="VLL06_06785"
     CDS             complement(3049..>5236)
                     /locus_tag="VLL06_06785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS30705.1"
                     /translation="SPDEDLRQVFVTAGSQLAQFFERKRAEASVHTYAKELEQKNRNL
                     DSALTEAQAATQAKSAFLAVMSHEIRTPMNGIMGMNGLLLETHMTPEQRDYAETVQRS
                     SEALLNIINDILDFSKIESGRMKLEMIDFDLRTTVEETLDLFAEPAQRKGLELGCLLH
                     AEVPTALRGDPGRLRQILANLIGNALKFTQQGEVMIHVARREEDAERSLIEFAVIDTG
                     IGITPEVQGILFKPFTQADTSTTRKFGGTGLGLAICKQLVEQMGGQIGIESVPGQGSA
                     FRFTVWLTQQPAQAQPMPMPRGSLAGRRLCIVDDNATNRRILEQYAFQWGLQSATASD
                     GYQALALLKDAAARGEPFDLAILDLQMPRMDGLELGRTITADPVLAGTRLVLLTSMGI
                     RGQAEKAKQAGISAYLSKPVHRSHLYDCLTMIVDLPAKSSMDSLEAGAASGPGHVLVT
                     KHVLKEAAVAARVRILVAEDNVVNQKVAVCQLEKLGYRADIVANGLEAVEAVSRVSYA
                     LVLMDCQMPEMDGLEATALIRKREGEQGSRRLPIIAMTANAMRGDREKCLAAGMDDYL
                     AKPAKLGQIETALARWLSSLSTPGEQKGPVPLVRQESAHECVDATVLAELRQLDMSCG
                     LLSTLITNFLRDAPTRLAVLQEALRQGDAQALARVAHDLNGSSGNLGVRKMRELCIEL
                     QALGRAKDLGKAGALLTQLVNEFELVRQRLMAEHATITPDTLADDI"
BASE COUNT         1083 a         1525 c         1403 g         1225 t
ORIGIN      
        1 gatggcatac cagcgaatcg caggcaccac ccccagatgg tcgagcaatg acggacgcag
       61 gtccaacgag agatcgcgga ctcgagcgag cagcgtgtcc agaatcccga aactgtcgag
      121 caacgaattc tgtaccgccg acatatccgc aacccgctgc atggtctgaa gattcatttt
      181 gagtgcggtg agcgcttgac ccacttcgtc gtgcagatcg tgggcgagct gccgacgttc
      241 ttgttcttgt aaatacatca attgccgtga aagcgcttgc aactgcgcgt gagtgtctcg
      301 tagcccctcc tctgcctccc ggcgagtcag ataggacgag aggctgtcgg ccagtgaatt
      361 gatcagattg cgttcttcaa caagaaatgg tccttcatgt tcctggggct tctcttccaa
      421 atacccgacc tctaaagatc cttggcgtcc cgcggaggtc gtcagtgccg cgcgttgcat
      481 ccagggtgtc tcccgaaatc gcggcgtcat tgcgaccact ccatcgatcc ggatgcatgc
      541 gaccgcaatg tccggatatt gccaggccgg cggtagcaac agtaccacgt gatgcagcac
      601 ctcattgagc gggacgctac tatcttgtaa cagccgcacc accccatgta acgccgtgag
      661 ctccttgacc cgttcatcaa gatgatgcaa caggtgttgg cgtttgtctt cgtcctctga
      721 aattgtccgt gacatactgc tatccgattg cgcgagggca acggctcgtt tccgtgcttg
      781 cctgcgcggg ggactcagtc agagaagcgc gtcgagccct cgcggaaggt ctgtgttctc
      841 actttatcct acatggaagg gatcggcagg aacaagtttc cgacagtacc ggagcctttg
      901 tgatcaatga agcgacatga gaaggacgag ccggctggat aaagcgaacc gacgtccgat
      961 ggacccctat cccgtcgtgt gacgcgatgg aatggtacca tcggaacgtc ggtgcggccc
     1021 ttcattggaa tcgtacaaac cccctatggt cagttcgttc ctactgaggc tgtacttttc
     1081 catgcagagc gttcgcctgt ctcaagtgtg cggcatacag ctcctgcatc tccttcgcat
     1141 caacccgctt ttcttgcgca gcggccgtta atgctgcacg cttaaacccc ttggtgtcgt
     1201 catagcgacc aatctttgag acggcggtct caaacttggc ggcttcggca tccaagtctc
     1261 gtgcctcatt ttgataaagc ctggccgccg cgagatgatc ctccgcggtg cctgtcgccg
     1321 ccagcttctc ctccaaggat gcggccattg ccccgttatt cccggtgaga ctgagcagcc
     1381 ccaccagtgg caacatggcc aatgctctga ttatgcggtt cctggatctc atcgctctac
     1441 ctccttgttc ggctgtccaa gacagccagt tgatctgttc ccgttcatgt gctccacctg
     1501 ctacccgatt ggtttgtccg cgatggattc acatgaccct ttcagttata cgtatacaca
     1561 aagggtctat gaatgcgtat caggggattt ctgagagttc caggaaaaat acctgaggtt
     1621 tgggcctatg gaaggagcgg atacatcgat gctctgactt ccgaagaagt catgtgttcg
     1681 caggacgagc tgtctcagaa aggggcaacg tcacagaatg tgagcgtcaa gcaccaagtt
     1741 gattcttcgc cgggggataa cctcccagca cagcgttgat cgctgcgaga agagactcac
     1801 tgtcaaaggg tttgtcgagg acatggtctg caccgagaac tttcgccgca gatgcgattt
     1861 ccatcatgcg caagtgtcct cctccggtca tggcaataat cttggtctga ggattcgatt
     1921 gtttcaactc cttgatgatc tcgatcccat cccggttcgg catgaagatg tcggtcagga
     1981 ctatgtccgg cgggttcttg tggaacatgg cgatgccggc ctgaccatcc gaggcgtcct
     2041 cgacgctgaa ccccgctcgg gtcagtatcg tcaccactgt cgaacggagc agctgttcat
     2101 catcgatgac gagaattcgc ggcataggac tcctccgagt aatagaaaga gcgccgggct
     2161 atacctttgg tcgtagcacg ggtgacgtag atgatggtgc ctcgaggact gtggatgtcg
     2221 atccgcggga ctggagctcg gaaattggag gcaaggcaaa ccctttgtgg agaaagtcgg
     2281 tcactcccag cgcccgcgct tcacgttcga ctgatgggct atcccctcct gtaaagatta
     2341 tgactttggc ctgagggtcg atggtacgga tttccctcaa caccgtgagc ccatcgatgt
     2401 ccggcatgtc gatatccaga atcgtcagat gcgggcgctc cattttaaat accgtgatgg
     2461 catcctttcc cttcgctgcc agtgccacat gacggctctc ctgtttgagc atggtctgga
     2521 ccaacgtacg gatactatcc tcatcgtcga cgatcaacac cttatatggc aacatagcct
     2581 gctccctttt attttataag gctccagcca agatcaactt cttaccaaca gctctccagg
     2641 ctttcagatg aggcgtcagg ttgttccgat ggcttgttgc accgctgcca ggagcttgtc
     2701 ttgatctact ggtttttgaa gaaacgcgac tgcgccggcc tcgatggtgc cggcttcagc
     2761 gaccagtggg tcctcagcgg tcacgatgat gatggggatt ccattcagag acgtattcgc
     2821 tttgaatctc tgcagaacca ccataccgtt ccctccagga agccctaaat cgagcaacat
     2881 cgcatccggc cgctccttct gcgcaacgga catcgcttgg agcgcgtcgg cggcaaagac
     2941 ggtgtgatac tgatgggctt tcaaccgcgc gctgagtagg aggcgggtgt ccgcattgtc
     3001 atcgacgatc aggattttct ttttatccat gggcatgcct caccttcctt atatatcatc
     3061 ggccagcgtg tcaggcgtga tcgtcgcatg ttcggccatc agacgctgac gtaccaactc
     3121 gaattcattc accagctgtg tcaaaagagc tcctgccttg ccgagatcct tggctctgcc
     3181 aagcgcctga agttcgatac agagctcgcg catcttccgt acgcctaagt tgccgctgga
     3241 gccgttcaaa tcatgagcca ctcgtgccaa agcctgggca tcgccctgcc gtagcgcctc
     3301 ctgcaagaca gccagacggg tcggcgcatc tctcaaaaaa ttcgtaatca gcgtggacag
     3361 gagaccacag gacatatcaa gttggcgcaa ttccgcgagc accgtggcat ccacacactc
     3421 atgagccgat tcttgtctga ccagcggcac cggtcctttc tgttcgccag gtgttgacag
     3481 gctggaaagc catctggcaa gggcagtctc aatttgcccc agcttcgccg gcttggcaag
     3541 atagtcgtcc ataccggccg ccaaacattt ctcacggtct cctcgcatgg catttgcggt
     3601 catggcgatg atgggcagtc gtcggctacc ctgttcccct tcacgcttcc gaatcaacgc
     3661 cgtcgcttct aatccatcca tctcgggcat ttgacaatcc atcaacacca gggcataact
     3721 cacccgtgag actgcttcga ccgcttcgag accgttggcc acgatatcag cccggtaacc
     3781 gagtttttcc agctgacaca ccgcgacttt ctggttcacg acattgtcct ctgccacaag
     3841 gatgcggacg cgcgccgcaa ctgcggcttc cttgaggaca tgcttggtca ccaacacgtg
     3901 gccgggccca ctcgcagccc cggcctccag cgagtccatg gaagattttg caggcagatc
     3961 cacaatcatg gtcaaacagt catataagtg cgagcgatgt actggtttgc tcagataggc
     4021 tgagatcccc gcttgcttcg ctttttcggc ctgcccacga attcccatcg aggtcagcag
     4081 gacaaggcga gtcccagcca gaacaggatc agcggtgatc gtgcgaccca attccagtcc
     4141 gtccatgcga ggcatctgta aatcgaggat ggccaaatcg aagggctctc ccctggccgc
     4201 cgcgtctttc agtagagcaa gggcctggta tccgtcggaa gcggtggcac tctggaggcc
     4261 ccattgaaac gcgtattgct ccaggattcg tcggttggtg gcattgtcgt ccacaataca
     4321 caaccgccgt cccgcgaggg accctcttgg catgggcatc ggctgagcct gcgccggttg
     4381 ttgagtcagc cacaccgtaa accggaaggc gcttccttga ccaggtacac tctcaattcc
     4441 gatctggccc cccatctgtt ccacaagctg cttgcaaatg gcgaggccca agccggtacc
     4501 gccaaacttt cgcgtcgtcg aggtgtccgc ctgagtaaac ggcttgaaca gaatcccctg
     4561 tacctcgggg gttattccga ttcccgtgtc aatcacggca aattcgatca ggctgcgctc
     4621 ggcatcctct tcccgtcgag ccacatgaat catgacttcg ccctgttggg taaacttcag
     4681 cgcgttgccg atgagattgg cgagaatttg acgtagacgg cccgggtcgc ctcgtaatgc
     4741 cgtgggcact tcagcgtgca gcagacatcc caattccaga cccttgcgct gcgccggctc
     4801 ggcaaagaga tccaatgtct cctcgactgt cgtccgaaga tcaaagtcga tcatttctaa
     4861 cttcattcga ccggattcga ttttcgagaa gtcaagaata tcgttgatga tgttgagcag
     4921 ggcttcactg gatcgctgca cggtctcggc gtagtcgcgc tgctcaggcg tcatgtgcgt
     4981 ttcgagcaag agtccgttca ttcccatgat gccgttcatg ggggtacgaa tttcatggct
     5041 catgacggca agaaaggcgg acttggcttg tgtggcggcc tgagcttcgg tcaaagcact
     5101 gtcgaggttg cgattcttct gctcaagttc cttggcatag gtgtgaacgc tcgcctccgc
     5161 ccgtttccgt tcgaagaact gagcgagctg gctcccagcg gtgacgaata cctgccgcag
     5221 atcttcatcg ggagag
//
LOCUS       DATIPF010000269         3830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_140160, whole genome shotgun sequence.
ACCESSION   DATIPF010000269 DATIPF010000000
VERSION     DATIPF010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140160"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(385..1380)
                     /gene="bioB"
                     /locus_tag="VLL06_06790"
     CDS             complement(385..1380)
                     /gene="bioB"
                     /locus_tag="VLL06_06790"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HSS30706.1"
                     /translation="MVDYQDFAAKALRGETLTRRECHAVLDSPDEQLLGLLQAAFTVR
                     SHYFGKTVRLQMLQNAKSGACQEDCHYCSQSAISTAPIERYNLLPRRQMIDGARQAAA
                     SKAQRYCIVISGRSPLDREIDEIAGAVRSIKEEVPIQICCSLGLMNEAQAKRLKAAGV
                     DRVNHNLNTSEAFHASICTTHTFQDRLTTIKNARAAGLEICSGGIVGMGEKNEDLIDL
                     AMALREVKPDSIPLNMLHPATGTPLEQCDNLTPQRCLKVLCLFRFLHPHTELRIAGGR
                     EHNLRSLQPLALYPADSVFVNGYLTTPGQPAPEVWSMIEDLGFTIEVDYRQPVNT"
     gene            complement(1464..2864)
                     /locus_tag="VLL06_06795"
     CDS             complement(1464..2864)
                     /locus_tag="VLL06_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS30707.1"
                     /translation="MLRLLTTIFLISVGIFLYSYFRELNPGTVAVRTGPAIQFELSPV
                     TLVVFSMAVGAVLVALAVGMRQTAHVIGNWRSTRLLRRKEKIDALHREGTHAFMSKRT
                     VEAIGLFEKALAMDPNRVDSLLWLGNIYRAENNFAEAIRLHQRANRVDDRNIEILLEL
                     ANDLEGAKRYEDALQALHKMLKIEPDNLTALIRKRDLLIRLEKWSEALEIQHRLLKAN
                     LPEPERLAVAHLLTGCTYEVGRQLLERGHPDKARRYFRGAIKKDRTFLPAYIGIGEIL
                     IREGKTKQAVEILKKIYAKTHSIIILHRLEELFLEQGEPSEIIRVYQEALRQDPNNPA
                     LQFYLGKLYYRLEMVDEAFDILSTVEGIQDQLIDYHKIMANLYLRKQHMEQAVAELKK
                     ALHFKKRVLVPYICTACQQESTEWSGRCRRCGTWNALVALPWPNAAQAASGQESGPLS
                     ISTVPYQGIASPFETV"
     gene            complement(2869..3330)
                     /gene="rsfS"
                     /locus_tag="VLL06_06800"
     CDS             complement(2869..3330)
                     /gene="rsfS"
                     /locus_tag="VLL06_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HSS30708.1"
                     /translation="MYFDIISTKRSAIVRTARSTALAIARAMRDKKATDALILQVAPL
                     TSVADYLVIGSADSDRQASAIADHISGVLSRSGSKPLSVEGRGASQWVLMDFGDVIVH
                     IFRQDVREHYALERLWADAKRITIPGEPPTTKPVVAPRLMRRKKVSAKMKT"
     gene            complement(3278..>3830)
                     /locus_tag="VLL06_06805"
     CDS             complement(3278..>3830)
                     /locus_tag="VLL06_06805"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30709.1"
                     /translation="TDTLKLFVKYAGGTANAVSDLVAALAISDVEIRRPGKSYSIDTI
                     RQLQQEYGPHTQLFFLIGLDAFLDFPSWRDPRTLLELCSFVVLSRPGLSFRSLSTVSL
                     LPPIPYPSLADLDAGRTFRIEAQIGGQRLICLKQPPCPISASDIRARIRQGLPVANLL
                     PPLVESYILRHHLYQEECNRTNS"
BASE COUNT          801 a          973 c         1189 g          867 t
ORIGIN      
        1 attcacctcc cctgcatcga gccagagtcc gtgtccggag gagcaaacat caagttccgg
       61 accttggctg gtcggaatct caacaagttc ctgctgacag tcggggcatt tcgtggtgag
      121 cattgtgtcc aaactgtagc aagaacctgg caggtgtcaa cgcgattgcc tcataaccga
      181 tttgaaaaac agaggattcg gcaggattgt cagagagtaa tcatgtggca gaccgaacga
      241 gctgacggct catcaacatg ctgcagcaga ccatcgctag agccgggtat ggtcgttctg
      301 gtagccagcg actggctccc ggctgggcaa caagcagccg atgacagggg ctgcgagatg
      361 aatgataaga agttggcgtg tgaattatgt gttgaccggt tgccggtagt cgacttcgat
      421 cgtgaagccc agatcctcga tcatgctcca gacctcgggt gccggctggc ctggtgtcgt
      481 gaggtacccg ttcacaaaga cggagtcggc tgggtagagc gcgagaggtt ggaggctgcg
      541 gagattgtgt tcccgtccgc cggcgatgcg gagttcagtg tgtgggtgga gaaatcggaa
      601 gagacagagc acttttaaac agcgttgcgg ggtgaggttg tcacactgct ccagtggagt
      661 gcctgttgct ggatggagca tgttcagtgg aatcgagtcc ggcttgactt cgcggagtgc
      721 catggcgagg tcgatcagat cttcgttctt ctcgcccatg ccgacgatcc caccggaaca
      781 gatttccaat cctgccgccc gcgcattctt gatcgtggtc aagcgatctt gaaatgtgtg
      841 ggtggtacag attgaagcat gaaaggcttc gctggtgttc aagttatggt tcactcgatc
      901 gacgccggct gctttcaagc gtttggcttg tgcttcgttc atcaagccga gcgaacagca
      961 gatctgaatc ggaacttcct ctttaattga gcgcacggct cccgcgattt cgtcgatttc
     1021 tcgatccaac gggcttcgcc cgctgatgac gatacaataa cgttgggcct ttgaggctgc
     1081 cgcttgtcgg gcgccgtcaa tcatctgtcg ccggggtaag aggttatagc gctcaatggg
     1141 tgcggtcgaa atcgccgatt gagaacagta atggcagtct tcttggcagg ctccgctctt
     1201 ggcattctgc aacatctgga gacggacggt cttgccgaaa tagtgagatc ggacggtaaa
     1261 ggctgcctgg agaagtccca gcagttgctc atctggacta tcgagcacgg cgtggcactc
     1321 gcgtcgtgtc agcgtctcgc cgcgcaaagc tttggcagca aaatcctggt agtcgaccat
     1381 gggacactcc atcatctgta gaggttcatt gacagcgatg cggatggctg cacgtgcgtc
     1441 aggcagccgg actggccaaa aacctacacg gtttcgaagg gagaagcaat gccctggtag
     1501 ggaaccgtgg agattgagag cgggccgctc tcttgaccgg aggctgcctg agcggcattt
     1561 ggccatggca gcgcaactaa ggcattccac gtcccacagc ggcggcagcg cccagaccat
     1621 tcggtcgact cttgttgaca ggcggtacag atatagggga cgagcactcg tttcttgaaa
     1681 tgtagggctt tcttgagttc ggccacggcc tgctccatgt gctgcttgcg aagatagaga
     1741 ttggccatga tcttatggta gtcgatcaat tggtcctgta tcccttccac ggttgagagg
     1801 atgtcgaagg cttcgtcgac catttcgagt cgatagtaga gcttgcctaa atagaactgc
     1861 agggctgggt tattcggatc ttgcctgagg gcctcctgat agacccggat aatttcgctg
     1921 ggttcaccct gttcgagaaa gagttcttcc aggcggtgca ggatgatgat gctgtgggtc
     1981 ttggcataga tcttcttgag aatttctact gcctgcttgg tcttgccctc tcggatcaag
     2041 atttcgccaa tgccgatata ggccggcagg aacgtccggt cttttttgat ggcgccacga
     2101 aagtaacgcc gagccttgtc ggggtgccct cgttccagta gttggcgccc gacctcatag
     2161 gtgcagccgg tgaggaggtg ggccacggcc aggcgctccg gctcaggcag gttggccttg
     2221 aggagacgat gttggatttc gagtgcctcg ctccattttt cgagtctgat caacagatcg
     2281 cgcttgcgga tgagcgcggt gaggttgtcc ggttcaatct tgagcatttt gtgcagggct
     2341 tgcaaggcat cctcatagcg cttggcgccc tccaagtcgt tcgccagttc cagtagaatt
     2401 tcgatgttgc gatcgtcgac gcgattcgca cgttgatgaa ggcggatcgc ttctgcgaag
     2461 ttgttttccg cgcgataaat gtttcccagc cagagcagtg agtcgacacg attggggtcc
     2521 atggccagtg ccttttcgaa taggccgatg gcttcgacgg tacgtttcga catgaaggca
     2581 tgcgtccctt cacgatggag cgcatcaatt ttttccttgc gccgcaacag ccgggtgctg
     2641 cgccagttgc cgatgacatg ggcggtttgc cgcatgccga ctgccaacgc cacgagcacg
     2701 gcgccgacag ccatggagaa gacgacgagt gtgacaggac tcagttcgaa ctggatggcg
     2761 ggaccggtcc ggactgcgac cgttccagga ttcaactcac ggaaataact gtagaggaag
     2821 atcccaacgc tgatgaggaa aatggtggtg agcagtcgga gcatcggtct aggtcttcat
     2881 tttggctgac acttttttcc gcctcatgag ccgtggggcg acaaccggtt ttgtggtcgg
     2941 cggttcgccc gggatagtaa tgcgcttcgc gtcagcccac agccgttcga gtgcgtaatg
     3001 ttcccggaca tcttgcctga aaatatggac aatgacatcg ccaaagtcca tcagaaccca
     3061 ctgcgaagcc cctctgcctt cgacgctcag cggtttggag ccggaacgag agaggacgcc
     3121 gctgatatgg tccgcgatgg cactcgcttg gcgatccgaa tccgccgaac ctataaccag
     3181 gtagtcggca acggaggtca gtggggctac ctggaggatg agagcatcgg tggccttctt
     3241 atcgcgcatg gcccgggcaa tggcgagggc cgtagaccta gctgttcgta cgattgcact
     3301 cctcttggta gagatgatgt cgaagtatat aggattccac taagggcggc aacagatttg
     3361 ccacaggaag tccttggcga atgcgggccc gaatgtccga tgcggagatc gggcagggcg
     3421 gttgcttgag gcagatgagg cgttggcctc caatctgcgc ctcgattcgg aaagtccgtc
     3481 ctgcatccag gtctgccagc gacgggtatg gaatcggcgg gagcaatgag actgttgaga
     3541 gggagcgaaa cgacaggcct ggcctggaga ggacaacgaa cgagcagagt tccaataacg
     3601 tccgagggtc acgccaggag ggaaaatcca gaaaggcgtc gagcccgatc aagaaaaaca
     3661 attgggtgtg tggtccatat tcctgctgca gctggcgaat ggtgtcgatg gagtaggact
     3721 tgcctggccg acgaatttcc acatctgaga tggcgagagc ggcgaccaga tcgcttaccg
     3781 cattcgcggt accgccggcg tacttcacga acagcttcag cgtgtcggtt
//
LOCUS       DATIPF010000270         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1645441, whole genome shotgun sequence.
ACCESSION   DATIPF010000270 DATIPF010000000
VERSION     DATIPF010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645441"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="VLL06_06810"
     CDS             complement(<1..955)
                     /locus_tag="VLL06_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HSS30710.1"
                     /translation="MPVQNFPPPRRLLLGPGPSMVHPRVLRALSTPLLGHLDPAFLAI
                     MDDVQTLLRSVFQTKNRFTIAVSGTGSAGMEASIVNIVEPGDSVIVGVNGVFGTRWAT
                     MVERCGGKVIQVEAPWGQVIEPEAVEQALRRSGPVKAVAIVHAETSTGAWQPLEAIAH
                     LCRDHDTLFIVDAVTSLGGAPVEIDRWGIDVCYSATQKCLSCPPGLSPFTLSDRALAA
                     VKARRTPCHSWYLDMALITDYWAEGTRAYHHTAPISMLYALREALRLVEEEGLPARFI
                     RHQLNSEALIAGLTELGLLPLSPTGQRLPMLTCVTVPAPIPE"
     gene            1220..2599
                     /locus_tag="VLL06_06815"
     CDS             1220..2599
                     /locus_tag="VLL06_06815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HSS30711.1"
                     /translation="MSALFLTVAFAVVAWLSASESKMDRIESPEQALSLMVSRTRDVQ
                     EGLKRAPQWEQQLFAWTSGNTETEQAHEIQWYQELAETSEDPLVPLQLAILQAEAGHV
                     SQALLSAHEWTGAEGPPPQFADLVVAAYGEGPVQDADRYGVWQAELAEVLPAGWFYDR
                     LAERLARRANNAELVARIQGEAAMRADRQFVWSHRLTLVELGAMALGTVVCALLWLRR
                     NEPSSFVRLHEPGVPPPWPGGVGAAVLLRGGAIGAMLSGLFLIFVPPDNVLLRVFVIP
                     MANAPLLLLAYRHLFRPSGMTFDAGFGLEIGWARVGGLLAAVSIAVAAGLWGEWVMDR
                     LSEPFHLTSHWAEWFDADLVWASPSLTAMSLIEYVIFAPLFEELAFRGLLFAILRRKF
                     SFLPAALISAGIFGVAHGYGLVGLLSVCWSGVLWAWIYEKTGSLLPGILAHAINNLLV
                     CLAVMALLR"
BASE COUNT          506 a          728 c          844 g          602 t
ORIGIN      
        1 cctcaggaat aggagcgggg actgtgacac aggtcaacat cgggagtcgt tgtcctgtcg
       61 gagaaagggg aaggagcccg agctcggtca agccggcgat caaggcttca ctattgagtt
      121 gatggcgaat gaagcgggca ggcaatccct cttcttcgac gagtcgcaac gcctcccgca
      181 gggcgtagag catggagatc ggcgccgtat ggtgataggc tcgagtccct tcggcccagt
      241 agtccgtgat cagggccata tccaggtacc aactatggca gggggtgcgc cgagctttga
      301 cagcggccaa cgcacgatcg ctgagggtga agggagacaa cccgggcgga cagctcagac
      361 atttctgcgt ggcgctatag cagacatcga taccccaacg atcaatctcg accggagcgc
      421 ctcccagcga tgtgaccgca tcgacaatga agagcgtgtc atggtctcga cagaggtggg
      481 cgatcgcctc aagcggctgc caggcaccgg tcgaggtttc ggcatgcacg atcgccacgg
      541 ctttcaccgg gccggagcgg cgcaatgctt gttcgacagc ctccggctct atgacctgtc
      601 cccatggggc ttccacctga atcactttcc cgccgcaacg ttcgaccatg gtggcccatc
      661 gtgttccaaa cacgccgttg accccgacga taaccgaatc gcccggctca acgatattca
      721 cgatcgatgc ttccatgccg gccgaaccgg taccggagac tgcgatggtg aagcgatttt
      781 tggtctgaaa gaccgagcgc aacagagttt ggacatcgtc catgatcgca agaaaggctg
      841 ggtccaagtg gccgagcaag ggagttgaga gggcgcgcag cactcgcgga tgcaccatgc
      901 tcggccccgg gccaaggagc agccggcggg gagggggaaa gttctgaact ggcatatcac
      961 tccggatgtg agaggggtat agtatagcgg agttcagtgg cattcagcca cgggtattcc
     1021 gaacgctctc cttcgctgcc gaccctctcc gagggaagtt cgagggtaaa aaaatccgca
     1081 tgttatagtt tcacggacga tggaacctac ccctccttct ctcaatgaac cgcatgagtc
     1141 tcggtcttgg tggcgggcct cagacgtacc accgccgagt ggggtctact ctgagcaatt
     1201 ctcacctacg gtcacgcatt tgtctgcact gttcctcaca gtggctttcg cggtggtggc
     1261 atggctgtcg gcatcggaat ccaagatgga ccggatcgag tcgcccgagc aagcgctgag
     1321 tctgatggtc agtcgaacta gggatgtgca ggaaggactc aagcgtgcgc cacagtggga
     1381 acagcaactt tttgcctgga cgtccggcaa tacggagact gaacaggcgc acgagattca
     1441 atggtatcaa gaactggcag agacatctga agacccgtta gtaccccttc agttggcgat
     1501 tctccaagcc gaggccggtc atgtttcgca ggcgctcctg tccgcgcatg agtggaccgg
     1561 agcagagggt ccgcctcctc aattcgccga tttagtggtg gccgcctatg gtgagggacc
     1621 agttcaagat gcggaccggt atggtgtgtg gcaagcagag ttggcagagg tattgcccgc
     1681 cggctggttt tatgatcgtc tggctgaacg cctggcacgt cgtgccaaca atgccgagct
     1741 tgtcgcgagg attcagggag aggctgcgat gcgggccgac cgtcagttcg tctggtcgca
     1801 tcgtctgacg ctcgttgaac tcggcgcaat ggctctcggc accgtggtgt gcgccctgtt
     1861 gtggctcagg cggaatgagc cgtccagttt cgttcggtta catgaaccgg gtgtgccgcc
     1921 tccgtggccg ggcggcgtcg gcgcagcggt gttgctgcgt ggaggcgcga tcggtgcgat
     1981 gctctctggg ctgtttttga tctttgtgcc accagacaat gttctactca gggtattcgt
     2041 cattccaatg gccaatgcgc cgttactcct cttggcctac cgtcatctct tccggccttc
     2101 tggcatgacg ttcgatgctg gatttgggct ggaaatcgga tgggctcgag tcggtggtct
     2161 gttggcggcg gtttcgatcg cggtggctgc tggtttgtgg ggcgagtggg tgatggatcg
     2221 gttgtcggag ccgttccatc tgacgagtca ctgggccgaa tggttcgatg cagatcttgt
     2281 ttgggcttcg ccctccctga cggcgatgag tttgatcgaa tatgtgatct ttgcgccgct
     2341 gttcgaagaa ctcgcgtttc gtggattgct gttcgccatt ttacgccgca agttcagttt
     2401 tctgccggct gcgctgatca gcgccggcat atttggggtc gcccatggtt atggattggt
     2461 gggactactg agtgtgtgtt ggagtggagt gctctgggca tggatctatg aaaagaccgg
     2521 aagcctcctt ccaggtattc tcgcccatgc gatcaacaat ttacttgtct gtctcgccgt
     2581 catggccctc ctccgctagc aggatggtga aaatgctttc cagcttcgat ctcggctcgc
     2641 tggaatcctc aacgtaccct cgagtgtacg cctccggttc
//
LOCUS       DATIPF010000271         3673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_846416, whole genome shotgun sequence.
ACCESSION   DATIPF010000271 DATIPF010000000
VERSION     DATIPF010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846416"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VLL06_06820"
     CDS             complement(<1..374)
                     /locus_tag="VLL06_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HSS30712.1"
                     /translation="METPYAILEIEESATPEEIKNAHRLLLQVWHPDRFHQQPKLLAK
                     AEEKTGKINKAFETLGNPGVKQQYDEWLRANRNWGVQPVGTVTCPSCQTSFSSGPERG
                     EKREFRANRHRGGKDSGHAAGA"
     gene            1033..1254
                     /locus_tag="VLL06_06825"
     CDS             1033..1254
                     /locus_tag="VLL06_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30713.1"
                     /translation="MSTRLTLPARRNHVTQKVKIASQRTLYISVHDDEQPAEIFLRLP
                     AEIESRISASARPIPDPINNAPNGVPKHT"
     gene            1680..2261
                     /locus_tag="VLL06_06830"
     CDS             1680..2261
                     /locus_tag="VLL06_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30714.1"
                     /translation="MNGIAKQKCMFYLLHGSRWAILVGLLVTGCTSDPPHYEGAFSAS
                     QQVKGNSDSVGAPLDLTWGVVLEVLAQQGFLIQQADTKSHIIMAHREMRSKEDKDLSH
                     TVSGTITVLPSGDPMTRILFAANQTTELHKKSYTWWHLLWLIPIFPTGTEYTTVVVER
                     DTVRSPQFYSDFFGAVKRAAEEKKEAAQLVPRS"
     gene            complement(2627..3256)
                     /locus_tag="VLL06_06835"
     CDS             complement(2627..3256)
                     /locus_tag="VLL06_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30715.1"
                     /translation="MRTRSLMLLVLLVTISGCIVTYRNFPNATLESLPKNHEPKPLYY
                     HIEPMGGTHLVWMPSLYPGFPHIDPIGQPGYHQVAQTLETSGLFSALIVVPDLPETGV
                     YCSIGFEKREPSALWFQAGGGDLRIAFLILLGLLPYYTGDGGTSVVYTLYRDGQLMQT
                     YRYPITKKGAAWIVLLPFAWLNFFTNDLKDAVRGTSLQFLIDTQRDGNF"
     gene            complement(3405..>3673)
                     /locus_tag="VLL06_06840"
     CDS             complement(3405..>3673)
                     /locus_tag="VLL06_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30716.1"
                     /translation="SAAQIQDSEDASSRGKAEVVIILCGAGGPPPMNASITVAAVSGS
                     GGAPTIQDGIECAQGLASVLSAGFHLRDVESGNVGGVLYTLIKR"
BASE COUNT          850 a          960 c          995 g          868 t
ORIGIN      
        1 cttgcaccgg cagcgtggcc ggaatccttt ccccctcgat gtcgattcgc gcggaactcc
       61 cgtttctcac ctcgctcggg accggaggaa aaggatgtct ggcaggaagg gcacgtcacg
      121 gtgccgacag gttgtacgcc ccagttacgg ttggcacgga gccattcatc atattgctgt
      181 tttaccccag ggttaccaag tgtctcgaag gccttattga ttttcccggt cttctcctca
      241 gctttggcga gaagcttggg ttgctggtga aagcggtccg gatgccagac ttgaagcaag
      301 agtcgatggg cgttcttgat ttcttcggga gtcgcggatt cttcaatttc tagaatggcg
      361 taaggggttt ccatactaaa cgctcccatc acgctccccg cagcgagcta cgggatattc
      421 ggcgaaagaa catacactga tcatggacat agccaccaag tcgcaatgga acaaggacaa
      481 tcaaagtttc atgggatatt ctagcgtagg atcgactcga actggaagga tatcgattgt
      541 aagaagcaca acccagctgg gtcatgggct gaacggttcc cctactgcac ctgtcgaaca
      601 gccctttctt cttccacccc actcctaaaa ccatgggctg aggcggttct gctctcaact
      661 gcgcacgtcc aacgagggcc tcctgaggcc gcgcgtctta gaatttcttc tcttcccatt
      721 ctcttacact attaacaacg gggtagggtc tgggtgtgtt tcgattgcgc gcattgaggg
      781 agcaccgccc gtaatttcac agtttaatca ttcttgtgtg cgcgctcagc gagcatggaa
      841 acacgcccag gccctacccc atcaccgccg acccatgatg atgaatcaag ccgataccct
      901 acctgaggac acccatgttg atccatcaag cgattcagag caggagaagc ggtcatgatc
      961 ggtgccacta gattagccta tatcatggtt gtttggcacc aacagagtgc gcgtcctgga
     1021 gaggagaggt ctatgagcac tcgcctgact cttcccgccc gtcgcaatca cgtcacgcag
     1081 aaagtgaaaa tcgccagcca acgtacgctc tatatcagcg ttcatgatga cgagcagccg
     1141 gcagaaatct tcctgcggct tccggccgag atagaatcgc gcatcagcgc gagtgcacgg
     1201 cccattccag accccatcaa caatgcgcca aatggcgtac ctaaacatac gtaatacacc
     1261 ttccactgtt atacgttcac tactaaccct taagatgggc ttgctaacgt taagtgcttt
     1321 tgagtatctt cccatccatt attagctagg aagacaatgc atgagaagta agaattctca
     1381 gcagagtggg cagcgcagtg ggtagagatc agggactaat gtgcggcatc gtcttgcgga
     1441 tgagaatttc ccggaagagt cgtgtgactc ttccgggaaa cctacaggct ggaaatgacc
     1501 tagcgggctt tggcaggggc atcacccctc gttgccgatg aagtctttga tagaaaattg
     1561 gtctctgggc tcattattgg gcaatcgatc cgtcttgcga tgtagtcact gcattgcacg
     1621 ccgcaatagg ccttgaatgc agaaaattca ctggtaacgg tgctagttag gagaacttca
     1681 tgaacgggat agctaagcag aagtgtatgt tctatttgtt acatggaagc cgttgggcca
     1741 ttcttgttgg tttacttgtt acgggctgta cctcggatcc gccgcattat gagggggcgt
     1801 tttccgcgag tcagcaggtc aaaggcaatt cggattcagt cggggcgccg ctggatctga
     1861 cgtggggcgt ggtgctggag gtgctcgcgc agcaggggtt tctgattcag caggctgata
     1921 ccaagagcca cattattatg gctcatcgag agatgcgatc caaggaagac aaggacctct
     1981 ctcatacggt gtccggcact atcactgtcc tcccgtctgg cgatccgatg acacggatat
     2041 tgtttgcggc aaatcaaacc actgagctcc ataaaaaatc ttatacctgg tggcatttgc
     2101 tctggctgat tccgatcttt ccgaccggca cggaatatac gaccgtagtc gttgaacgtg
     2161 atacggtgcg cagcccgcag ttctacagcg acttttttgg agcggtgaaa cgagccgcgg
     2221 aagagaaaaa ggaagccgct caattggtgc ccagatcgta gattcaacga gggcgaaaga
     2281 gctggtccat gagcaccacc ctgccttcac cagtcgtgat tgatattggt cccgtgaggc
     2341 acgagcacgt cggcttgaga gtgtgagcgt gacttggttc ctatacacga aagcagctgc
     2401 ggcatgcttg gctcgcgtgc cgtaggcctg ccccttctcc ggacagtcga tgccgctgag
     2461 gcggatacgt tcagggcgtt ggttatggag gacttcgatc gtctcgccgt cgagaacgga
     2521 gacaacgggg ccggagaagt tggcagcaca gacaatagtt gggatgagca aggattccag
     2581 cagcaggatt ggggtcctgt gcgggatcat ggtcgttaca gggctgttag aaattgccat
     2641 cacgttgggt gtcgatgaga aattggagcg aggttccccg caccgcatct ttcagatcgt
     2701 tggtgaagaa gttgagccag gcgaaaggca atagcacgat ccacgctgca cctttcttgg
     2761 tgataggata gcgataggtc tgcatgagtt gaccatcgcg gtacagggtg tagacaacgg
     2821 atgttccgcc gtcccctgta taataaggta aaagaccaag cagtattaaa aaagctattc
     2881 ggagatctcc tccgcccgcc tgaaaccaca gagctgaggg ctccctcttt tcaaagccga
     2941 tgctgcaata gactcctgtt tctgggagat ccggcaccac aatcaaggca gagaacaacc
     3001 cactggtctc caacgtctgt gccacttggt gatatccagg ttgccctatt ggatctatat
     3061 gaggaaatcc aggatacaag cttggcatcc aaaccaagtg ggtgcccccc ataggctcta
     3121 tgtgatagta cagtggcttc ggctcgtggt tcttcggcag tgattccaac gtcgcgttgg
     3181 ggaaattccg ataggtcacg atacagccgg agatggtgac cagcagtaca agaagcatga
     3241 ggctgcgcgt tctcacggat tatttctgct tcaccacaat cttgtacttg atgtgttcat
     3301 aagtcgctcg cggaagaatc ctcttgggcg ggtctgtttc ctccagtgcc ccggatgttt
     3361 gatcgcctga tcccccttgc accaccggca ttcctctcct agcgttacct tttgattaag
     3421 gtgtatagaa ctccgccgac attgccgctt tccacatcgc gtagatggaa acccgcagac
     3481 aacaccgatg cgagtccttg agcacactca atcccgtctt gaatcgtggg tgcgccacca
     3541 ctgccactga cagcagcgac agttatgctt gcattcattg gagggggtcc acccgcccca
     3601 cagagaatga ttacgacttc tgccttacct cggctggaag cgtcttcgga atcctggatt
     3661 tgggcagcag acg
//
LOCUS       DATIPF010000272         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_51060, whole genome shotgun sequence.
ACCESSION   DATIPF010000272 DATIPF010000000
VERSION     DATIPF010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51060"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VLL06_06845"
     CDS             complement(<1..237)
                     /locus_tag="VLL06_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30717.1"
                     /translation="MRLKSLVWACLLYFPVGVVGQTTTVSIAPDAPSKEDVRKLFDIM
                     SSKEQIHQMMEQMFQQMRTMSREQMKKRRPEVTDE"
     gene            complement(272..652)
                     /locus_tag="VLL06_06850"
     CDS             complement(272..652)
                     /locus_tag="VLL06_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSS30718.1"
                     /translation="MKVLLDTHAFLWAITDESRLSTKARAVIANADSWFSVASVWEVV
                     VKVQSGKLSLPQPVGEFLTSELALSRVQILPIALNHVLRVESLALHHRDPFDRILIAQ
                     SLEEKLPLVTADPLFDRYRVELIW"
     gene            complement(653..880)
                     /locus_tag="VLL06_06855"
     CDS             complement(653..880)
                     /locus_tag="VLL06_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HSS30719.1"
                     /translation="MEVNIHEAKTHLSRLLERVAMGEEVVIAKAGKPVAKLVPLSSGK
                     GKFKFGSAKGKFAIPDDFNDPLPKEIEDLFW"
     gene            complement(925..>2033)
                     /gene="eno"
                     /locus_tag="VLL06_06860"
     CDS             complement(925..>2033)
                     /gene="eno"
                     /locus_tag="VLL06_06860"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HSS30720.1"
                     /translation="GKGTLKAVENVNGEIAEALANLDGFDQRGLDAKMIELDGTENKG
                     RLGANAILAVSMAAARASAVSLDIPLYRYLGGAGANTLPTPMMNILNGGAHADNNVDF
                     QEFMVMPVGAPSFSEALRWGVEVFHTLKGVLKKRGYNTAVGDEGGFAPSVKSNEEAIE
                     VVLEAITKAGYKPGAEIAIALDPAASEFFQDGKYVFKKSDKSVKSSDEMIKVWAKWVN
                     NYPIVSLEDGLAENDWDGWQNLTKELGGKIQLVGDDIFVTNIQFLQEGIDKGVANSIL
                     VKVNQIGTVSETLDAIDLARRNGYTSVISHRSGETEDTFIADLAVATGAGQIKTGSAS
                     RTDRISKYNQLLRIEEELGGTARFLGIKALNYKG"
BASE COUNT          457 a          606 c          470 g          500 t
ORIGIN      
        1 ctcgtctgta acctcgggac gccgtttctt catctgctca cggctcatgg ttcgcatttg
       61 ctggaacatc tgctccatca tctgatgaat ctgttccttg ctcgacatga tgtcgaagag
      121 cttcctgaca tcctctttag agggggcgtc gggggcgatt gagactgtcg tggtctgccc
      181 taccacgccg accgggaaat atagcagaca cgcccagacc agtgacttca atctcataaa
      241 tcctccgact acaggaattc gtttctcgca gttaccaaat caactcgacc cgataccggt
      301 cgaacaatgg atccgccgtg accaaaggaa gcttctcttc gaggctttgc gcgatgagaa
      361 ttcgatcgaa tggatcacgg tggtgaagag caagactttc cacgcgcagc acatggttca
      421 aggctatggg aagtatttga actctgctta aggccaattc agaggtaaga aactccccca
      481 ccggttgcgg tagagacagt tttccagact gaaccttgac gacgacctcc cagacgctgg
      541 ccacgctaaa ccacgaatcc gcattggcga ttactgcccg tgcttttgtt gataaacgcg
      601 actcgtccgt tatggcccaa agaaatgcgt gggtgtccag taagactttc atttaccaga
      661 acagatcttc aatctccttc ggcaacggat cattaaaatc gtcggggatt gcgaattttc
      721 cttttgccga accgaacttg aacttacctt tcccagatga gagaggcaca agtttggcta
      781 cgggtttgcc cgctttggcg atgacaacct cttcacccat cgccacccgt tccaacagcc
      841 tggaaaggtg ggttttggcc tcgtggatat tcacttccat ttaactatgg tagactaagt
      901 ccttagctaa gtctacatca aagatcagcc cttgtagttc agcgccttga tccccaaaaa
      961 tcgcgccgta ccgcccaatt cttcctcgat tcgcaacaat tggttgtact tggagatacg
     1021 atccgtacgc gatgctgacc ctgtcttgat ttgacccgct cccgtggcga ccgccagatc
     1081 cgcgatgaat gtgtcttcgg tctcgccgga gcgatgcgaa attaccgagg tgtatccatt
     1141 gcgacgcgct agatcgatcg cgtccagcgt ctcgcttacg gttccgatct ggttgacctt
     1201 gaccagaatt gaattggcga cgcccttatc gattccctct tgcagaaact gaatattcgt
     1261 cacaaagatg tcatcgccaa cgagctgaat cttcccaccc agctctttgg taagattctg
     1321 ccaaccgtcc cagtcgttct cggccagacc gtcttcgagc gacacaatgg gatagttatt
     1381 cacccacttc gcccagacct ttatcatctc gtcggaactc ttcaccgact tatccgattt
     1441 cttgaagacg tacttaccat cctggaagaa ctcgctggcc gcgggatcga gcgcaatggc
     1501 aatctccgca cccggcttgt agccagcctt ggtaatggct tccagcacga cttcgattgc
     1561 ctcctcattc gacttcactg aaggcgcaaa cccaccctca tcgccgactg ccgtgttgta
     1621 accgcgcttc ttcaacacgc ccttcaaggt atggaaaacc tcaacgcccc aacgcagcgc
     1681 ttcagaaaac gaaggcgcgc ccaccggcat caccataaat tcctggaagt cgacgttgtt
     1741 gtcggcgtgt gctccgccgt tcagaatgtt catcattggc gtcggcaacg tattcgctcc
     1801 cgcaccgccg agataccggt acagtgggat atccaaactc accgctgaag cacgagcggc
     1861 agccatggag acagcaagaa tcgcattcgc accgagccgg cctttatttt ctgtgccgtc
     1921 cagttcgatc atcttggcat ccagccctcg ctgatcgaat ccatccaagt tcgcaagtgc
     1981 ctcggcaatc tcgccattga cgttctctac tgctttcagc gtgcccttgc cga
//
LOCUS       DATIPF010000273         4345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_225330, whole genome shotgun sequence.
ACCESSION   DATIPF010000273 DATIPF010000000
VERSION     DATIPF010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225330"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(358..1158)
                     /locus_tag="VLL06_06865"
     CDS             complement(358..1158)
                     /locus_tag="VLL06_06865"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HSS30721.1"
                     /translation="MNKPKPAPLRERDITRQIAREYYKEFDQLIESDVIIVGAGPSGL
                     ICAHDLADMGFRTVIVEQSLALGGGFWHGGYLMNKATICAPAHKILEEIGVPCKKIKD
                     CDGMYIVDPPHATGALVAAAYRGGAKVLNLTRVVDLILRREGVLEGVVVNNTTAEMAG
                     HDFIHVDPIGLESKIVVDATGHDAVVVNLLHKRNLYTAVPGNGAMWVSRSEEDVMNHT
                     GEVYPNCFVIGLAVSAVYGTPRMGPAFGSMLLSGRYGAELIKKKLKQE"
     gene            complement(1297..3210)
                     /gene="thiC"
                     /locus_tag="VLL06_06870"
     CDS             complement(1297..3210)
                     /gene="thiC"
                     /locus_tag="VLL06_06870"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HSS30722.1"
                     /translation="MSNKTIGNGAATGNGHRSAESALTTTPFPASRKVYVQGTQVGVR
                     VPMREISLTATKSMNGGTPTPNEPITIYDTSGPYTDPDVTIDAQAGLAPYRRNWVIGR
                     NDVVELSDISSQYGRARAADPKLDELRFQHIRKPFRAKPGMNVTQIHYARKGIVTPEM
                     EFIAIRENQSHEVARELASRNGHDGGVIQHPGQSWGASIPSVITPEFVRDEVARGRAI
                     IPANINHPESEPMIIGRNFLVKINSNIGNSAVASSIEEEVEKMIWSIRWGADTVMDLS
                     TGKNIHETREWIIRNSPVPIGTVPIYQALEKVGGKAEDLTWELFRDTLIEQAEQGVDY
                     FTIHAGVRLAYVPMTAKRMTGIVSRGGAIHAKWCLAHHEENFAYTHFEEICEIMKAYD
                     VSFSLGDGLRPGSIADANDEAQFAELDTLGQLTTIAWRHDVQVMIEGPGHVPMHMIQV
                     NMEKQLKACREAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLG
                     LPDREDVKTGVITYKIAAHAADLAKGHPGAQVRDNALSKARFEFRWEDQFNLSLDPDT
                     SRLFHDETLPDNAAKVSHFCSMCGPHFCSMKITQDVRDYATQLQVDEQTAIQIGMKEK
                     SEEFKKAGSEIYR"
     regulatory      complement(3307..3409)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3577..>4345
                     /gene="nadA"
                     /locus_tag="VLL06_06875"
     CDS             3577..>4345
                     /gene="nadA"
                     /locus_tag="VLL06_06875"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HSS30723.1"
                     /translation="MQTLSTLPKPITDYQGLSAEELFRRTAEAKRVLGDRVMILGHNY
                     QRDEVIEHADFRGDSLLLSKLAAERSERPYVVFCGVHFMAETADILSRSKQTVILPDM
                     AAGCSMADMAAIEQVDQCWETLGRILPVEETVMPAVYVNSAAVLKAFCGEHGGITCTS
                     SNAKAVIEWSWARREKVLFFPDEHLGRNTANKMGLPREAMIVWDPYQPNGGNTREAIQ
                     RAKLILWKGHCSVHQMFQPVHVDNFRKRYPDGKVIVHP"
BASE COUNT          844 a         1218 c         1271 g         1012 t
ORIGIN      
        1 tcaaggacct cttgatgcaa ctgatcgcgg ccttggttgg aaagcccgat ttcagcgcca
       61 tccaattcgc gcttatcttc ttcgcggcct tcgccttgga tcaagaaatc ctgtacgtcc
      121 atagtaatca ggctgatgaa aaatgtcccc agcgtcgttc tcagtcgtgc gaactcctca
      181 acggggaccc ggccgcctca ctaactcggc ggcgcgcaca gacgtggtgc tccttattcg
      241 tcgcaccgtg cgccccagag ggtacgcctc cgggttcgca ctccctgcgg ccttgctgga
      301 tgaccttttt gatcagcctg taggccgccc gttgattccg actagaacta actataccta
      361 ttcctgcttc agcttcttct tgatcaactc cgcgccgtag cgcccggaga gcaacatcga
      421 gccgaaggcc gggcccatgc gcggtgtgcc gtagacggca gacacggcca atccgatgac
      481 gaaacaattg ggatagacct cgccggtatg gttcatcacg tcttcttccg agcgcgagac
      541 ccacatggcg ccgttcccgg gaacggcggt gtagagattg cgcttgtgca acagattgac
      601 cacgacggca tcatggccgg tggcatcgac gacgatcttg ctttcgagcc cgatcgggtc
      661 gacgtgaatg aaatcgtggc cggccatttc ggcagtcgtg ttgttgacga caacgccttc
      721 caggactccc tcccggcgga gaatgaggtc gacgacgcgc gtaaggttca gaaccttggc
      781 gccgccgcga taggctgcgg caacaagggc gccggtggca tgcggcggat cgacaatgta
      841 catcccgtca cagtccttga tcttcttgca agggaccccg atttcctcga ggatcttatg
      901 tgcgggggcg cagatcgtcg ccttgttcat cagatacccg ccgtgccaga accctccgcc
      961 gagtgcgagg ctctgttcga cgataacggt acgaaagccc atgtccgcca agtcatgggc
     1021 gcaaatcagg ccggatggac ctgcgccgac gataatgaca tcgctctcga tgagctgatc
     1081 gaattctttg tagtactcgc gtgcgatctg tcttgtaatg tcgcgttcgc gtaagggggc
     1141 tggttttggc ttgttcatac tatattctcc ttcggcggct gctgaaaatg gcttccggct
     1201 tcgttctcag ctccaccaaa tcctcaacgt accccagagg gtacgcctcc ggtttggtct
     1261 cgcctgcggc cttgccggaa accattttga gcagccttat cgataaatct cggagccggc
     1321 ttttttgaac tcttccgact tttccttcat gccgatttga attgctgtct gttcatcgac
     1381 ctgtaattgc gtggcgtagt cccggacatc ctgcgtgatt ttcatcgagc agaaatgagg
     1441 gccgcacatc gaacagaagt gtgacacctt cgcggcgtta tccggcaagg tttcgtcgtg
     1501 gaagagcctt gaggtgtcag ggtcgagtga aagattgaac tggtcttccc agcgaaactc
     1561 gaaacgggct ttggacaggg cgttgtctcg aacctgcgcg cccggatggc ccttggccaa
     1621 atccgctgcg tgggcggcaa tcttgtaggt gatgacgccg gtctttacat cttcgcggtc
     1681 ggggagtccc aggtgttcct tgggcgtcac atagcagagc atcgcacagc caaaccatcc
     1741 gatcatggct gcgccgatgc cggaggtaat gtggtcgtag cccggtgcga tgtcggtcgt
     1801 gaggggtccc agtgtataga agggcgcttc ccggcaggcc ttcaactgtt tctccatgtt
     1861 cacctggatc atgtgcatgg gcacgtggcc tggtccttcg atcatgacct gtacgtcgtg
     1921 gcgccaggca atcgttgtga gctgccccaa ggtgtccagc tcggcgaatt gtgcttcatc
     1981 attggcatct gcaatggagc ctggacgcaa cccatcgccc aaactgaacg acacatcgta
     2041 ggccttcatg atctcgcaga tttcctcgaa gtgggtgtag gcgaagtttt cttcatgatg
     2101 agccagacac catttcgcat ggatcgctcc gccgcgggac acgatgccgg tcatgcgttt
     2161 ggcggtcatc ggcacatagg cgaggcgcac gcccgcgtgg atggtgaagt agtcgactcc
     2221 ctgttctgcc tgctcgatga gcgtgtcgcg aaagagctcc caagtcaggt cttcggcctt
     2281 gcctccgact ttttccagcg cttgatagat gggtacggtg ccgatgggca ccggcgagtt
     2341 acggataatc cattcgcggg tctcgtgaat gttcttgccg gtggagagat ccattacggt
     2401 atcggcgccc catcgaatgg accagatcat cttttcgact tcttcttcga tggaggaggc
     2461 gaccgcagag ttgccgatgt tggaattgat cttcaccaaa aaatttcggc cgatgatcat
     2521 gggctcgctt tccgggtggt tgatgttggc cgggatgatc gcgcggccac gggcgacttc
     2581 atcgcggaca aattccgggg taatcacaga gggaatactg gcgccccatg actggcccgg
     2641 gtgttggatg accccgccgt catgtccatt ccgtgaggcc agttcacgtg ccacttcgtg
     2701 ggattggttt tcccggatgg caatgaactc catttccggt gtgacgatac ccttcctggc
     2761 atagtggatc tgagtcacat tcataccggg tttcgctcgg aagggcttgc ggatgtgttg
     2821 aaaccgcaat tcgtccagtt ttgggtccgc tgcacgtgcg cggccatact gtgaggaaat
     2881 atccgagagc tcgaccacat cgttcctgcc gatcacccag tttcgtcgat agggcgccaa
     2941 tccagcctgc gcgtcgatgg tgacgtccgg atctgtatag gggccggagg tatcgtagat
     3001 cgtgatcggc tcatttgggg tgggggtacc cccattcatt gacttggtgg cagtcaggct
     3061 gatctctctc atcggaaccc gcacgccgac ctgcgtcccc tgcacatata ctttgcgtga
     3121 ggcagggaaa ggtgtagtcg tcagggctga ttcggcagac ctgtgtccgt taccagtggc
     3181 agctccattg ccgatggtct tgttgctcat ggcggagatc ctttctccaa aacgtgaact
     3241 gtgttcagtc gtcaaaaata aaaaaccgca cctgatgttg aggggtgcgg tagagagacc
     3301 aacggtgtct ggttgttctc gcttccctac gctggtatta cccaggtcag gttctgaggg
     3361 tcgtccgttt gggcggactc tcagccgtgt ggccccccta gcttcgacgg ggaatcgtat
     3421 cgccggcctg cttcgttgtc aacggggtat taggcctacc atagccgaac cgaccggact
     3481 atcgaggggc taggactcct tcgcagcccc tgggttcatt gattgtgggg aatccctatc
     3541 gtagaatagc cctatacgcc aattcgcaag gacacgatgc agacactttc gacacttcca
     3601 aaaccgatca cagactatca gggactgtct gcggaggaac tgttccggcg gacggctgag
     3661 gcgaaacgtg tgctcggtga tcgagtcatg atcctgggcc ataattatca acgggatgaa
     3721 gtcatcgaac acgcggattt tcgtggggac tcccttctac tctcgaagct ggcagctgag
     3781 cggtcggagc ggccctacgt cgtcttttgt ggcgtccact tcatggccga gaccgccgat
     3841 attctcagcc gctcgaagca aacggtgatc cttcctgaca tggctgccgg ctgctccatg
     3901 gcagacatgg cggccatcga acaagtcgat caatgctggg aaacgctggg gcgaatcctt
     3961 cctgtggaag agaccgtgat gcccgcggtc tatgtgaact ctgctgcagt gctcaaagcg
     4021 ttttgcggcg aacatggcgg gattacctgt acgtcctcca acgcgaaggc ggtgattgaa
     4081 tggtcatggg ccaggcgaga gaaggtcctc tttttcccgg atgagcactt gggccgcaat
     4141 acggccaaca agatggggct gccgagagag gccatgatcg tctgggaccc ctatcaacct
     4201 aacggaggca atacgcgtga ggcgatccag cgcgcaaagc tcatcctctg gaagggccat
     4261 tgcagcgtgc accagatgtt ccagcccgtg cacgtagaca atttccgcaa gcggtatccg
     4321 gatgggaagg tgatcgtcca tccgg
//
LOCUS       DATIPF010000274         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_51293, whole genome shotgun sequence.
ACCESSION   DATIPF010000274 DATIPF010000000
VERSION     DATIPF010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51293"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..1395)
                     /locus_tag="VLL06_06880"
     CDS             complement(247..1395)
                     /locus_tag="VLL06_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HSS30724.1"
                     /translation="MKASSSPQSQRATVFIAASETDANLYYATKFIAPDPFIYLEIKG
                     ERILVMSDLEMDRAKSQSSVDRVLSYSEVEGRAKTQGIAEPGTVEVVHILLRESGVTQ
                     IQVPVNFPFGHAVKFQSLGYQLFPKRDPFYEQRVVKTAEEVRFIEVAQRATEQAVAAA
                     HDMLRQASINHDQLWFDGMPLTSERVKKLINVRLMECDCVAQHTIVAGGEQACDPHNE
                     GSGPLPAHRSIIFDVFPRSATNRYFADMSRTVVRGTPSPELKRLYQTVLDAQEEAITK
                     VKDGADGKRIHQGICARFEKAGYQTGLVNGRMQGYFHGTGHGVGLDIHEMPRISRTGS
                     LLQDGHVVTVEPGLYYPGLGAVRIEDMVLVTKDGCRNLTNSPKTFELG"
     gene            complement(1434..>2754)
                     /locus_tag="VLL06_06885"
     CDS             complement(1434..>2754)
                     /locus_tag="VLL06_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HSS30725.1"
                     /translation="SMSRVPEWIAKGGFEAGNIELSLDMKGKDLDWKTWRTTGWFALT
                     NGLMNAKGAQGPIQDLYLRVHLVKNGVDIKRLSFRLLDSDLVLDGTVRNWAVKPMITA
                     KIESNQMDLDLLIPKGERSPIREFLETLAATSKVQATASIARGHYKHLKFGSLSARIM
                     IQDGVLDLDRVSGQSTNGEVAGRVVVQLPRKQPADAEISLRATGLPVEDVLKLLGPKG
                     EFMTGEARMTGTIRGHGRNPHGLYPTLNGKVDILLENGRILKSQERAVWKIISILNLP
                     AVLQGKVDLEKEGLPYNKITATVVAQNGLFETENLIIDSPIVKITAAGNFDLPTDQVD
                     MVWAVSPFGSYSQFLKTIPLFGRLFAGDRKGIATALFSVKGAIEDPEVTYLPMKSFAT
                     GVTGLAQLAFDILKNTVMLPIDLMTPEEDKGTSKDTIPAPEPVPSVP"
BASE COUNT          588 a          806 c          725 g          635 t
ORIGIN      
        1 gatcgcggag cggcaggaat agcggagcgc cacactcccg tcacactcgt tcttgatgcg
       61 aatgagggca tcgaggacgg tggtcccacg tgcaacctcg aggcggaagt cttgaggatg
      121 ggcggcggaa tcgacctcgg gattgaatcg ttggatcgtg aaggtcaggc gcataggacg
      181 cgttcagcct ttcgcgatca gccatcagca agctgaaagc tgatcactga gagctgatcg
      241 ctcatcttaa cccaactcga atgtctttgg agagttcgtc agattgcggc atccatcttt
      301 cgtgacaagc accatgtcct caatgcggac ggccccgaga ccgggatagt acaggcccgg
      361 ctcaaccgtc acgacgtggc catcttggag tagtgacccg gtgcggctga tacgcggcat
      421 ttcatgaatg tcgagcccga caccatgacc ggttccgtga aagtatccct gcatgcgacc
      481 gttcaccagc cctgtctggt acccggcttt ctcaaacctg gcgcagattc cttgatgaat
      541 tctttttcca tcggccccgt ctttcacttt cgtgatggct tcttcctgtg catcgaggac
      601 cgtttggtac agtcgcttca actctgggct tggagtcccg cgcaccacgg ttctggacat
      661 gtcggcgaaa taccggttgg tggctgagcg aggaaacaca tcaaagataa tgctgcgatg
      721 cgccggtaat ggtccactac cttcgttgtg cgggtcgcag gcctgctctc caccggcgac
      781 aatcgtatgc tgggcaacac agtcgcactc catcaatctc acattgatca gtttcttcac
      841 tcgctccgac gtcagcggca tcccatcgaa ccacagttga tcgtgattga tcgaagcctg
      901 acgcagcatg tcgtgggctg ccgcaaccgc ctgttcggtc gctcgctggg cgacctcgat
      961 aaagcggact tcctcggcgg tcttcaccac gcgctgttcg tagaacgggt cccgcttcgg
     1021 aaacagctga taccccaggg attggaactt gacggcatgg ccaaagggaa agttcacagg
     1081 aacctgaatc tgcgtgacgc cggactctcg cagcaggata tggacgactt caaccgtgcc
     1141 tggctctgcg attccttgcg tcttggcccg cccttcgacc tcagaatacg agagcacccg
     1201 atcgacggac gactggctct ttgcccgatc catttcgaga tcgctcatga cgagaatccg
     1261 ttctccttta atctccaaat agataaaggg gtccggcgca ataaacttcg tcgcgtaata
     1321 taaattggcg tcggtttcgc tggccgcgat aaagacggtg gcacgttgag attgcggact
     1381 ggatgaggcc ttcatgaacg gtcctcggaa tctagcacgg agggggaggt cggtcaaggt
     1441 acggacggaa ccggttcagg cgctggaatc gtatccttgc tcgtaccttt gtcctcctcg
     1501 ggtgtcatca aatcgatcgg cagcatcacc gtgttcttca agatgtcgaa cgccagttgc
     1561 gccagacccg tcacgccggt ggcgaacgac ttcatcggca ggtaggtcac ctccggatcc
     1621 tcgatggccc ctttgacaga gaacaaggcc gtggcaatac ctttacgatc accggcaaag
     1681 agccgtccaa agagtggaat cgttttcaga aattgagaat aggatccgaa cggactgacg
     1741 gcccagacca tgtcaacttg atccgtcggc aaatcaaaat tgcctgcggc ggtaatcttg
     1801 acgatcggac tatcgataat caggttctca gtttcgaaga gcccattttg cgcaacaacc
     1861 gtcgccgtaa tcttgttata gggcagccct tccttctcca ggtccacctt cccctgcagc
     1921 accgccggca aattcaatat gctgatgatt ttccagaccg cacgttcttg tgatttcagt
     1981 atccgcccat tttcaagcag gatatccacc ttgccgttca acgtaggata cagcccgtgc
     2041 ggattcctcc catgcccgcg tatcgtccct gtcatccgag cttcccccgt catgaattct
     2101 cctttggggc caagtaattt cagcacatcc tccaccggca aacccgtcgc tcgaagcgag
     2161 atctccgcgt cggctggctg cttacgtggc agttgcacca cgacacggcc ggcgacttct
     2221 ccattggtcg attggccgga tacacgatcc aaatcgagca cgccgtcttg aatcataatg
     2281 cgggcggaga ggctcccaaa tttgagatgt ttgtaatggc cgcgcgcaat cgatgcagtt
     2341 gcctggacct tgctggtggc ggcaagggtt tccagaaatt cccggatagg cgaccgctcg
     2401 cctttcggaa tgaggagatc gagatccatt tgattggact cgatcttggc ggtaatcata
     2461 ggctttaccg cccagttgcg gacggtcccg tccaacacta gatcactgtc gagcaggcga
     2521 aacgacagcc gtttaatgtc tacgccattc ttcacgagat ggacccgcaa atacagatcc
     2581 tggatgggtc cttgggctcc tttcgcattc atcagaccgt tggtcaacgc gaaccatcct
     2641 gtggtcctcc aggttttcca atcaagatct ttccctttca tgtcgagcga caattcaata
     2701 ttccccgcct caaaccctcc cttcgcaatc cattccggca cgcgggacat tgac
//
LOCUS       DATIPF010000275         2130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_225565, whole genome shotgun sequence.
ACCESSION   DATIPF010000275 DATIPF010000000
VERSION     DATIPF010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225565"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="VLL06_06890"
     CDS             <1..1088
                     /locus_tag="VLL06_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSS30726.1"
                     /translation="AARFHQSNRSFLSTTMKAIQVSSLGSSHMEVIGVVGVAAIIWYG
                     GFLVINGSMTPGAFFSFLTAMFMAYAPIRKLSGSNNQIQQALAAAERVFGVLDLKTEQ
                     EAERGQLELPRITQSVTFDDVTFHYESQAVPALNDINLTIRAGEMVALVGSSGSGKTT
                     LVNLIPRFYEPTAGRILIDGVDIQSYTLRSLRSQIGMVSQDVVLFDDSIRNNIAFGRE
                     HATDEDIIRAARLAYAHDFVERFPQGYQTIVGEKGVKLSGGERQRLAIARAILRDPPL
                     LILDEATSALDTESERVVQLALANLMKNRTTVVIAHRLSTIQQADRIMVLARGSIVEV
                     GTHDELLRRGGQYQRLHAMQFQDVPDA"
     gene            1081..1713
                     /locus_tag="VLL06_06895"
     CDS             1081..1713
                     /locus_tag="VLL06_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HSS30727.1"
                     /translation="MRNPALKRVETWVKLSVFPPIGAAVIRGLGWSMRMDSQGHEQVD
                     ALYRQGRHIILAFWHAQQLMIPTGYRGTGANVLISQHQDGEIIARIISRFGHWAVRGS
                     STRGGAFALRELIRRGRSGGDLVVTPDGPKGPRQVAKLGVVQLAKATGLPIVPLAFSC
                     SKKNSSRAGIDSWSRTHGPEASICGAPPSGSRETPMNRHWRRHAWSLRRS"
     gene            1835..>2130
                     /locus_tag="VLL06_06900"
     CDS             1835..>2130
                     /locus_tag="VLL06_06900"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HSS30728.1"
                     /translation="MWRLFYNILLLAVSPVIVAVLLAKQRCRRGLLYRLGLKNSLSGL
                     SGLSGAPGRDRPDRPARPVIWIHAVSLGEVVAVTPLVIELHRRHPECRLVVSTVT"
BASE COUNT          413 a          581 c          651 g          485 t
ORIGIN      
        1 aagcggcacg atttcaccag agcaaccgtt cgtttctcag tacgaccatg aaagcgattc
       61 aggtgtcttc ccttgggtcg tcgcatatgg aagtgattgg tgtcgtgggg gttgcggcga
      121 ttatttggta tggcgggttc cttgtgatca acggctcgat gacgccgggg gcatttttct
      181 catttctgac ggcgatgttc atggcctatg cgccgattcg gaaactatcc ggttccaata
      241 atcagattca acaagccttg gctgcggcgg aacgggtctt cggcgtccta gatctcaaga
      301 ccgaacagga agcggaacgg ggtcagttgg agttgccgcg gattacacag tcggtgacct
      361 ttgacgatgt gacgtttcac tatgagagcc aggcggtacc ggctctcaac gacatcaacc
      421 tgacgattcg cgccggagaa atggtggccc ttgtcggcag cagtggcagt ggaaagacga
      481 cactggtcaa tttaatcccg cgcttctatg aaccgacagc cggccgcata ctgatcgacg
      541 gggtcgatat tcagtcatac acgctccgct cgctccggtc gcagatcggg atggtgtcgc
      601 aagatgtcgt cttgtttgac gacagcattc gcaacaacat tgcctttgga cgtgaacatg
      661 ccaccgacga agacatcatc cgagcagctc gattggcgta tgcccatgac tttgtcgagc
      721 gttttccgca gggataccag acgatcgtgg gtgagaaagg cgtcaaactc tccggagggg
      781 agcggcagcg cttggccatt gcccgggcga tcttgcgcga tcctcccttg ctcatccttg
      841 atgaagcgac gtcggcattg gacaccgagt cagaacgggt cgtccagttg gcgctcgcca
      901 atttgatgaa gaatcgaacg accgtcgtca ttgcccatcg gttgtccact attcaacagg
      961 ccgatcggat tatggtgttg gcccgaggct cgattgtcga agtggggaca catgacgagc
     1021 tgcttcgacg tgggggacag taccagcggc ttcatgcgat gcagttccag gatgtgccag
     1081 atgcgtaacc ccgccctgaa gcgggtcgag acatgggtga aactttctgt gttcccgccg
     1141 atcggggctg cggtgattcg aggactgggg tggtcgatgc ggatggattc gcagggccac
     1201 gagcaggtgg atgcgttgta tcgccagggc cggcacatca ttctcgcgtt ttggcacgcg
     1261 cagcagctca tgatccccac aggttatcgc ggaacaggtg ccaacgtgtt gatcagccag
     1321 catcaagacg gtgagattat cgcgcgcatc atctctcgat tcgggcattg ggcagtccgt
     1381 ggatcgagca ccaggggcgg cgcgttcgcc ctgcgtgaat tgatccgccg tggccgctcc
     1441 ggtggcgatc ttgtggtgac ccccgatgga cccaaggggc ctcgccaggt tgcgaaactc
     1501 ggagtggtcc aactggcaaa ggcaaccggg cttccgattg tgccgttggc gtttagctgc
     1561 tcaaaaaaaa actcttcgcg agctgggatc gattcatggt cccgtaccca tggtcccgag
     1621 gcctctatct gtggggcgcc cccatctgga tcccgcgaga ctccgatgaa caggcattgg
     1681 agaaggcatg cctggagctt gagacggtcc tgaatcgact gacggatcag gcggatgccg
     1741 cctgcaaggt ctagtaggag ggcccaccct tctcacctgt cttgcacttc ttgcgtcacg
     1801 gtccatggtt gatggctgac cactgacagc tcatatgtgg cgtctcttct acaatattct
     1861 cttgctggcc gtttcgccgg tcatcgtggc ggttctgctg gccaagcaac gctgccgccg
     1921 cggattgctg taccggctgg gactcaaaaa tagtctatct ggtctgtccg gtttatctgg
     1981 tgctcctgga cgagacagac cagacagacc agcccggcca gtcatttgga ttcacgctgt
     2041 gtctctcggt gaagtggtcg cagtgacacc gcttgtgatc gagctgcatc gccgccatcc
     2101 cgagtgtcgc ctggtggtct cgacggtgac
//
LOCUS       DATIPF010000276         2635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_407258, whole genome shotgun sequence.
ACCESSION   DATIPF010000276 DATIPF010000000
VERSION     DATIPF010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407258"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VLL06_06905"
     CDS             complement(<1..219)
                     /locus_tag="VLL06_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30729.1"
                     /translation="MNHQPSAHYSMDYCGGAIGAAGILSELLWPTGGTMIAGVGFAET
                     DEDSVLDGVVGVTGIVVSTAGIGAPLSVE"
     gene            306..2624
                     /gene="priA"
                     /locus_tag="VLL06_06910"
     CDS             306..2624
                     /gene="priA"
                     /locus_tag="VLL06_06910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HSS30730.1"
                     /translation="MISTQAIPPHTQPEFLFADIIVPRHLAGPFTYAVPLFLRPTLRI
                     GHRVLVPFGRSILQGAVIALSHVLPQGLDPARLKEIHSLAPDGAAPDVSSNLFQLSRQ
                     VAEQYVAPWGQCLRLVLPPAPIARVQSGRYELTEKGRAALATREPCSVKARALLTRLG
                     KKPSGLHGSSRNPIPTDVLHDLKARGWVMEVQDLSSASTVPLVQPFGQASHSSITAPL
                     IHDPTLSWAEPLFKALDGQGPVRVLVQAPWADRLKLLQQTMLLVLDRRQTVLIIVGEA
                     ERAQRVADLVRDDEAGISPICFHSGLSDRARAEMWDQIHRQAVRVVVGTRSAIFLPLT
                     AIGAIWIEGEEDAALKEAQEPRYHAREVAWLRVQDDPAVLVLSSSHPSLEIKAAVEQR
                     GIVVSKSIASDARPSIQVVDLRDHGRGTVLSHPLLQAVGRAIGRRAGVLLFLNRKGYA
                     GALVCRDCGQVPRCLTCRVALMYSRQAGRLLCSYCGYRAPIPDTCVSCSSPRMQVIGE
                     GTERVEEDAKRLFPHAAVIRVDGDTMRRPAQAEAVWRRIEQGEWDIIVGTQLLLRHGT
                     LPTMGLVGIVQADAGLSVPDFRSAERTYHTLLDAVDLTDSTQAGGQVILQTYLSSHHA
                     IQAVAQNNESIFISEELSHRMALGYPPAVYLIALLVSGTNEKIVRDAALAWVARLTDF
                     ASSSMAGHTVMGEAPSTAQSMNPPDHLTVLGPVPSPVPRLRGRYRWQILVKSLKRETG
                     LAAVRVTVNDMERTYQRRAIKFDVDVDPVEMW"
BASE COUNT          551 a          767 c          721 g          596 t
ORIGIN      
        1 ctcaacggat agcggcgctc cgatccccgc agtagacact acaatcccag tgactccaac
       61 gactccgtcc aagacagagt cttcatctgt ctctgcgaat cctacgccgg cgatcattgt
      121 gccaccagtt ggccagagca attcagataa gatccccgct gctccaatcg ctccaccaca
      181 gtaatccatc gagtagtgtg cggaaggttg atgattcaac gtcaaccctc cgcggttccc
      241 cctcagaact tttccatccc ctctctctca ttgttcccgc ttttccggtg ctgttacaat
      301 gccgcatgat ttcgacgcag gcaatccctc cacacacaca gcctgaattc ctatttgcag
      361 acatcatcgt cccacgtcat ctggcaggcc cgttcaccta cgctgtgcct ttgtttctcc
      421 gacccacgct gcgaattgga catcgagtcc tcgtaccctt tggacgatcg atcctccaag
      481 gggcggtgat cgcactctct cacgttcttc ctcagggtct tgacccggca cgcctcaagg
      541 agattcattc gttagcccct gacggggccg caccagatgt atcctcgaat ctattccagc
      601 tctcacggca ggtcgccgaa cagtatgtcg cgccatgggg acaatgcctc agattggtgc
      661 tacctccggc acctattgca cgagtacaga gcggacgcta tgaactcacg gagaaaggac
      721 gggccgcact tgcgactcgc gaaccctgtt cggtgaaagc acgagcactt ctcacacgtc
      781 ttgggaaaaa accttctgga ctgcacgggt cgtcccgtaa tcctattccc accgacgtgt
      841 tgcacgatct caaggctcgt ggatgggtca tggaggtcca ggacctctca tctgcttcga
      901 cggtgcctct cgtccaacca ttcggacagg cgagccattc cagtatcaca gcaccactca
      961 ttcatgaccc gacactatct tgggcagaac cactattcaa ggcgctggac ggccaaggac
     1021 cagttcgggt tttggtccag gctccctggg ctgatcggtt gaagctgttg cagcagacta
     1081 tgctcctggt gctcgaccgt agacagacgg tccttatcat cgttggcgaa gcagaacggg
     1141 cccaaagggt tgcagacttg gtacgtgatg acgaggcagg catctccccg atttgttttc
     1201 atagcggtct ctcagaccgg gcgagagccg agatgtggga tcagattcat cggcaggccg
     1261 ttcgagtggt cgtggggacg aggtcggcca tctttctccc cctgactgcg atcggggcaa
     1321 tttggatcga gggagaagaa gacgcagcgc tcaaagaagc acaagaacca cgctaccatg
     1381 cacgggaagt ggcttggtta agagtacaag atgacccggc agtgctggtc ctgagctcat
     1441 cccatccctc acttgagatc aaagcagcag tggaacaacg aggaatcgtt gtaagcaagt
     1501 ctatagcgtc tgatgcgcga cccagcatac aggttgttga tttacgcgat catggtcgcg
     1561 gtacggtact gagccacccg ctccttcaag ccgtcgggcg ggcgatcggt cgccgggccg
     1621 gtgtcctgtt gtttctcaat cggaagggct atgccggtgc cttggtctgt cgtgactgcg
     1681 gccaagttcc ccgctgtctt acctgtcgtg tcgcgctgat gtactcccgg caagcaggtc
     1741 gtttgctctg ttcctactgc ggatatcgtg cacccatccc cgatacctgt gtctcctgtt
     1801 ccagtcctcg gatgcaggtg attggggagg gcacagagcg ggtggaagaa gatgcgaaac
     1861 gattgttccc tcatgcggca gtaattcggg ttgacggaga taccatgcga cgaccagcgc
     1921 aggctgaggc cgtctggcga aggatcgagc aaggtgagtg ggatatcatc gtcggaacac
     1981 agctgctgct gcggcatggg actctcccga cgatgggcct cgttgggatt gtgcaggcgg
     2041 acgcaggact cagcgtgccg gatttccgat ctgccgaacg tacctatcat acactcctgg
     2101 atgcggtcga tctcaccgat tcgacacagg ctggcggtca ggtgatccta caaacctatc
     2161 tgtcatcgca tcatgcgatt caggctgtgg ctcagaacaa cgaatctatc ttcatatcag
     2221 aggagctgtc ccatcgaatg gcattagggt atccaccggc ggtttatctg attgccctct
     2281 tagtatcggg caccaacgag aaaatagttc gtgacgctgc tttggcctgg gtcgctagac
     2341 tcaccgattt tgcttcatca tccatggctg gacacacagt catgggagag gccccttcga
     2401 cggctcaatc gatgaatccg ccagaccatc tcactgttct ggggccagtc ccttcaccgg
     2461 ttccgagact gcgtgggcga tatcgatggc aaattcttgt gaaatcgctc aaacgggaaa
     2521 cgggactcgc cgcggtgcgg gtcacagtca acgacatgga gcgaacgtat cagcgccgag
     2581 ccatcaagtt cgatgtcgac gtcgatccgg tcgagatgtg gtaggtgtta cttgt
//
LOCUS       DATIPF010000277         3296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_141609, whole genome shotgun sequence.
ACCESSION   DATIPF010000277 DATIPF010000000
VERSION     DATIPF010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141609"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..537)
                     /locus_tag="VLL06_06915"
     CDS             complement(109..537)
                     /locus_tag="VLL06_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30731.1"
                     /translation="MEHLLEYAGSLHIYLGPYRGNPIALYLKRTEAGCQIGPKVYPWN
                     GVVGNGETPNKAAADFEEKWKSNGLSTDMYSGASWEGGIKPERPAPPKPPAAPKPAAP
                     AATSAPAGQPSAPTAPPAAPTDNTPASAPTLVPEPADTSS"
     gene            808..2187
                     /gene="rimO"
                     /locus_tag="VLL06_06920"
     CDS             808..2187
                     /gene="rimO"
                     /locus_tag="VLL06_06920"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HSS30732.1"
                     /translation="MVSRLITPSRSGSKKTTIGFVNLGCSKNQVDSEIMLGTLVTDGF
                     ALTADPQKAEVVIINTCGFIEEAKQESIDTIIAHGKLKQSGSCRILIAAGCLAQRYQG
                     DLLKQLPELDGVVGTGEFGKIAEICRDLLAPKKRQQRLWISEPPYLYDADAPRLRLGQ
                     AHSAYVKIAEGCNRNCAFCAIPLMRGKQRSRPVESIVAEVTRLAAEGVKEVNLISQDT
                     VNYGVDLGVRHGLVTLLRELVKVKDLRWIRPFYLYPQQVSNELLDLYAGEEKITKYID
                     MPLQHINDRMLKRMHRLGDRAAITSLVDRIRTRIPGVTFRTAFIVGFPGETEQAFEEL
                     RNYVEEAEFDRVAVFLYSDEEDTPAVGLDDKIERSVMDERRNEILAVQEGIAAARGQA
                     RIGTVMEVLVEGVSEETGLLLEGRHEGLAPEIDGVVYINDGSAAAGDLVKVEITDAAL
                     YDLVGHIVS"
     gene            2377..3240
                     /gene="purU"
                     /locus_tag="VLL06_06925"
     CDS             2377..3240
                     /gene="purU"
                     /locus_tag="VLL06_06925"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HSS30733.1"
                     /translation="MAHRKESVVLLIHCKDRRGIVARVSGFIHDFGGNILDSDHHTDE
                     ETNEFLMRMEFAAEGLQIPPDEIAAAFAPVAKTFEMRFEVRPSSRRTKVAILVSKQDH
                     CLADLLQRHKRDELHIEIPVIISNHDTCAGWAELFKIPFSVCPVTKETKPQQEQHVLS
                     LLKEHRVELVVMARYMQILSAGFLSQVGCPVINIHHSFLPAFIGANPYRQAYDRGVKI
                     IGATAHYATDDLDEGPIIEQDVIRVGHRDTVDDLVRKGRDLEEIVLARAVRRHVERRV
                     LVYGRKTVVFD"
BASE COUNT          733 a          896 c          944 g          723 t
ORIGIN      
        1 tctcgctcga cgatcctgcg agatcgagac ctgtcgttcc agacatgcgg ataattagat
       61 tgccggaagt ccgacatcat tttccacagc ctgcttatgc aacgtgagtt agctcgatgt
      121 gtctgctggc tcaggcacca aggttggagc agaagccggt gtattgtcgg taggagcagc
      181 cggaggggct gtaggcgcgg aaggttggcc tgctggcgcc gatgtcgcag cgggtgccgc
      241 cggcttcgga gctgcaggcg gcttgggagg agcaggtctt tctggcttga tacctccctc
      301 ccacgaagcg ccggagtaca tatccgtcga gagaccgttt gacttccact tctcctcaaa
      361 atccgcggcc gccttattcg gcgtctctcc gtttccgacg acgccgttcc acggatagac
      421 cttcgggccg atctggcacc cggcctccgt ccgctttaaa tacaaggcga tcgggttccc
      481 gcggtacggt ccaagataga tatgcaggga accggcgtac tcgagcaaat gctccatcct
      541 cgccctccag gaggggggca tgatgccata agagagaacg ggagggcaag gaggttgctc
      601 cccttgctcg cgcaacacgc gccctcagaa gggcctcgtt ggacgcgcgc agtcagggag
      661 caaacctccc cgccctctag ggagtggaga agatgaaaat atgcatcttg aactgagcag
      721 atcatgtgtc cactgtagga aaatcggccc ctgtctcaag gtgctattga ttgacaggct
      781 tttcaaccgt cccgtagact gccccctatg gtttcacgat taatcacccc atctcgctca
      841 ggcagcaaga agacgacgat cggtttcgtc aacttgggtt gttcgaaaaa ccaggtcgat
      901 tctgaaatca tgctgggtac cttggtcacg gacggatttg ctttgaccgc tgacccacag
      961 aaagccgaag tggtcatcat caatacctgc ggtttcatcg aagaggccaa gcaggagtcg
     1021 atcgacacga tcatcgcaca cggcaagctg aaacaatcag ggtcttgccg tatattgatt
     1081 gccgccggtt gtttggcgca acgatatcaa ggagacctgc tcaagcaatt gcctgaactc
     1141 gacggggtgg ttggaaccgg tgagttcgga aagatcgcgg agatttgccg ggatctgttg
     1201 gcgccgaaaa agcggcagca acggctctgg atcagcgaac ctccctacct gtatgacgca
     1261 gacgcgccgc gtctgcgtct cggacaggcc catagtgcct atgtaaaaat tgcggaaggc
     1321 tgtaaccgca attgtgcctt ctgcgcgatc cccctgatgc ggggtaagca gcgaagccgt
     1381 ccggtagaat cgatcgtggc tgaagtgacc cgtctcgctg ctgaaggtgt gaaagaggtc
     1441 aatctcattt ctcaggatac ggtcaattat ggcgtcgatc ttggtgtgcg ccacggcctg
     1501 gtcacgctgc ttcgcgagtt ggtgaaggtg aaggacttgc gctggatcag gccgttctat
     1561 ctctatccgc aacaggtatc gaacgaactg ttggatctct atgcaggaga agaaaagatc
     1621 accaagtata tcgacatgcc actccaacat atcaacgatc ggatgcttaa acgcatgcac
     1681 cggctcggtg atcgggccgc tatcacctcc ctcgtcgatc ggatccggac acgcattccc
     1741 ggcgtcacgt ttcgtacggc cttcattgtc gggtttccgg gagagacgga acaggcgttt
     1801 gaggagctca ggaattatgt ggaagaggcc gagttcgatc gggtcgcggt atttttgtat
     1861 tcggacgagg aagacacacc ggccgttggg cttgacgaca aaatcgaacg gtcggtcatg
     1921 gacgagcgaa ggaatgaaat cctggctgtg caagaaggga ttgctgcggc acgaggccaa
     1981 gcccgtatcg gcacggtgat ggaggtattg gtcgaagggg tctcggagga gacagggtta
     2041 ttgctcgagg gacgccatga agggctggct ccggagatcg acggcgtcgt gtatatcaat
     2101 gatggatcag ccgcagcagg ggacctcgtc aaggttgaga tcacggatgc agctttgtac
     2161 gatcttgtag gccacatcgt ctcctaagca ggatcctcaa aaagtccgcc agcggcgttc
     2221 tcgcttcctt cagaccctca acgcaccaca agggtacgcc tcgggccctc actcgctgcg
     2281 gccttgctgg acagcctttt tgaggatcct gttagcacca ttttcctcgc ctgctacaat
     2341 tgaattgacc cctctcattg gaaagaaagc cccacaatgg cacaccgaaa agaatctgtg
     2401 gtcctcctga ttcattgtaa ggatcgcaga ggtattgtgg cccgtgtctc aggcttcatt
     2461 cacgacttcg gcggaaacat tctggattcg gatcatcata ccgatgaaga gacaaacgag
     2521 tttctcatgc gcatggagtt tgccgccgaa ggtttgcaga ttccacccga tgagatcgcg
     2581 gctgccttcg ctccagtcgc caaaacgttc gagatgcgct ttgaggttcg cccttcaagc
     2641 cgccgaacga aggttgcgat attggtctcg aaacaggacc attgtctggc agatctactc
     2701 caacggcaca aacgcgacga gctgcacatt gaaattcccg tgattatatc caatcacgat
     2761 acctgtgccg gctgggctga gctgtttaaa atccccttct ccgtctgtcc cgtgacgaaa
     2821 gaaaccaagc cgcagcagga acaacacgta ctctccttgc tgaaggaaca ccgtgtcgag
     2881 ctcgtggtga tggcccggta catgcagatc ctcagtgccg gctttctttc acaggtcgga
     2941 tgtccggtca tcaatattca tcactccttc ctgccggcct tcatcggcgc taatccctat
     3001 cgacaggcct atgaccgggg tgtgaaaatc atcggagcga cggcgcacta tgccacggac
     3061 gatctggatg aaggtcccat catcgagcag gacgtcatac gtgtgggaca ccgggacacc
     3121 gtcgatgatc tcgtgaggaa gggacgggat ctggaggaga ttgtcctggc ccgggctgtg
     3181 cgccgccatg tcgaacggcg ggtcttggta tacggcagaa aaaccgtggt attcgactaa
     3241 ccggatgctc caaaagtcta ccagtttcgt tctcgcatcg ttcagaccct caacgt
//
LOCUS       DATIPF010000278         6970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_759344, whole genome shotgun sequence.
ACCESSION   DATIPF010000278 DATIPF010000000
VERSION     DATIPF010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759344"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VLL06_06930"
     CDS             complement(<1..257)
                     /locus_tag="VLL06_06930"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS30734.1"
                     /translation="MKLGHPILKDNNQKAHSLLDRRYAERVPIRYRITYSGEEGARIV
                     RGEGALKDLSKTGCKILGAPTISLGSRLTLFLDLEDGQAPMR"
     gene            complement(571..1110)
                     /locus_tag="VLL06_06935"
     CDS             complement(571..1110)
                     /locus_tag="VLL06_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30735.1"
                     /translation="MSRRSYAAPWIRGTSLLCLLFSIILQNTLVFAVPMQNDPNGFEG
                     IPWGATFSETDTFKKVEDAGPSQTYELKTGVPSLGPVKVDSLRFVTSEGKFARVTVRY
                     QGKATHDQILAYLQSLYGLLDRTPGQIASGPVKFYSWTGFETDVTLRYETGTDRGIIF
                     FESQEFRRKITEGNSPTVF"
     gene            complement(1112..1645)
                     /locus_tag="VLL06_06940"
     CDS             complement(1112..1645)
                     /locus_tag="VLL06_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30736.1"
                     /translation="MIGRQSHHANRYLVLLGFLLGMGCPLLAHAVPMVNDPNGFQSLA
                     WGTALTTRPDLEVARAGPHINEYQLRDALPVFDGIPVESLRFLSVDEQFARVTIRYQG
                     NDRHKQILTHLEEQFGFLERVPGQMMRGLTQQYNWRGTNTEINLTYQANTERGFIFID
                     SRTLAPRFNDHITDSAE"
     gene            1768..2265
                     /locus_tag="VLL06_06945"
     CDS             1768..2265
                     /locus_tag="VLL06_06945"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HSS30737.1"
                     /translation="MIESDAFVQALQDIGVDFFTGVPDSILGGIIAELMERQLYTPAV
                     REDEAVAMAAGAYMSGKVPAVLMQNSGLGTSLNALISLNMIYCQPCILIVSWRGYQGK
                     DAPEHVVMGEVMPEFLDTMKIPHRTLSEKTAIDDLKWLAETFMKQRIPVALLMTKGVV
                     RGLHP"
     gene            2262..2840
                     /locus_tag="VLL06_06950"
     CDS             2262..2840
                     /locus_tag="VLL06_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSS30738.1"
                     /translation="MRPEQGTMQSRAMAMAALLELLTDQPVIICNGFPSREAYKIADR
                     PTHFYMIGSMGNAPAIALGVALAKPNKQVVVFDGDGNVLMGMGTLATVGALKPKNFIH
                     VVFDNEVYGTTGNQPTISNVVPLEKVAKAAGYVNVERVREREDLVYEFKDMLKKDGPS
                     MLLVKVNEMVEDVGRVLLDPPDITKRFMKAIE"
     gene            3011..4093
                     /locus_tag="VLL06_06955"
     CDS             3011..4093
                     /locus_tag="VLL06_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSS30739.1"
                     /translation="MVLLNPGPVNVSERVRQALLHPDVCHRESEFTELLHGIQAKLLK
                     LFVPGAEADYAAVVLTGSGTAAVESAVMSSLPHGKRMLVLNNGVYGERISQMIGLYRL
                     GVSELKYDWTTRPDPERLRLALRQHPEAHAVAMVHHETTTGLINPVKEIADVVDSQNR
                     VFILDAVSALAGEQLDIAGSHIYMVAGTAGKCIQGFPGVSFVLVNKGFLERMRTYARR
                     SIYLHMTQYVDDEGRGTIPFTPAVQVYYAFDEALNELMEEGIAKRIQRYRKVATLIRD
                     RMAKLGVKPLLTPDKQSSTITAYFLPEGMTYQVLHDRLKEEGYVIYAGQGQLQSKIFR
                     VANMGALTEAQFVGFLDAFERICGTA"
     gene            4090..4809
                     /locus_tag="VLL06_06960"
     CDS             4090..4809
                     /locus_tag="VLL06_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HSS30740.1"
                     /translation="MKAIILAAGVGKRLWQVTQHRPKCLIEIGGQTLLHRYLTSLRSV
                     GVQSADMVVGYKQEMIRAAVTADSCGVRVNFLVNEQFHRGSISSLWIAREALDDDAIV
                     MDADVLFHQEILRRLVQSPYENALLMDDTVKQTGEECMVVVEGGRVIALTKKMPLRYD
                     YAGEGVGFLKVRHADTPYVVASLKTHVDHEAWQMEYEDALLGFFRDVKVGHEKIGGLP
                     WTEIDFIEDVSKAERDVLPKL"
     gene            4912..6339
                     /locus_tag="VLL06_06965"
     CDS             4912..6339
                     /locus_tag="VLL06_06965"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HSS30741.1"
                     /translation="MSESVVQKQTGLQGLSTAILLPSVSLFGEPAGLYTDEVGPLTSV
                     VGIGLFQRAVLTLQRAGIRQLMVLVGPEESQLRQALAKGPRVTIPVRWMPIREFPLDD
                     PRTWEALAAEVRGFCLLSGVQAVFASPLIESLRQSVQEGQAIVVARADSGRDRQPRRS
                     NLRVKVQSGRLLSITSRQDDTALVVTDLVVLPASFMSVVGQASGEVGTVPIRRWLERA
                     AVDGHVRVLSTETNRSHWYQDVHDAADVKAAEKKLFNSLKGEFEGFVDRFFNRKVSRW
                     FTRIFLAAGFSPNAITIVASLIGLAAAAGFGLGTYSAGIIAALLFQLAAIIDCCDGEV
                     ARLTFTESPFGAWLDIAMDNVVHMAIFAGIAVGSYLHLAGSDGAWVSLALGAAAVLGN
                     GLSFWLVTRAQKIKAASGWKTPVQAAWSDFMLKNVASRDFSVVVLIFAVLGKLDWFLW
                     MAAIGSVVFSILMLWVIRPSATSRA"
     gene            6332..>6970
                     /locus_tag="VLL06_06970"
     CDS             6332..>6970
                     /locus_tag="VLL06_06970"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HSS30742.1"
                     /translation="MLKGFLLVVGLLTLGFLVWHIGPERIYDAASQLGPVALLILLIP
                     STIMYVVEAYGWKITLGPSAKHIPFWRVLAIRTAGEVVNMTTPTAYVGGEPVKAYLLN
                     RHHVPMVKGLASVVIAKTTMTIAEVVFILLGLALAFWRVGADGSSGQTVAAALVSVGL
                     LVLVTAALVFVQQQGLFTWLLGVQRKLGLKIHYLEKREKKLLSLDRTILDFYR"
BASE COUNT         1425 a         1771 c         2202 g         1572 t
ORIGIN      
        1 cgcatggggg cttgcccatc ctcaaggtcg aggaacaggg tgaggcggct ccccagcgag
       61 atcgtgggcg cgcccagtat cttgcacccg gttttggaga gatccttcag ggctccctct
      121 cctctaacga ttcgtgcgcc ctcttctccg gaataggtaa tgcggtagcg aattggcact
      181 cgttcggcgt agcgccggtc caaaagcgaa tgcgctttct ggttgttgtc cttgagaatt
      241 ggatgcccca gcttcatcgg tttctcctca gcggttccac ggcggttctt ctgtcccccg
      301 cgccagcctg agaataccgc atccaggaat ttcgaccatc cctcaggagg ggggaatgat
      361 gtcccaccag aagggggggg ggagtttagg gatgagcctt aactggcgcg ggtttcctgg
      421 cttttgagcc gcacgtgaca cgcgagactg gcggaaaggt gaaaacggat gaactagggt
      481 tggcgcgtcc cgcacatcag tcactggcag gatttcccag catcctgcca tacgtttcgg
      541 agggtgaggc gagagatgga gtgacgagga tcaaaacacg gtgggtgaat taccttcggt
      601 gatttttcgg cggaattcct ggctctcgaa aaagataatg ccgcgatcgg tgcccgtttc
      661 gtatcggagg gtcacatcgg tttcgaagcc ggtccagcta tagaatttga cagggccgct
      721 ggcgatctgt cccggtgtgc ggtcgagcaa gccatagagg gattggagat aggcgagaat
      781 ctgatcgtgg gtcgccttgc cttgatagcg gacggtgaca cgcgcgaatt tgccttcaga
      841 tgtcacgaat cgtaaggagt caaccttcac cgggccaagc gaaggaaccc cagttttcag
      901 ttcataggtt tgtgacggcc cggcatcctc gactttcttg aatgtgtcgg tttccgaaaa
      961 tgttgcaccc caggggattc cctcgaatcc gttcgggtcg ttctgcatcg gcacggcgaa
     1021 taccagggtg ttctgtagga taatggagaa gaggagacag agaagggagg tgccacgaat
     1081 ccagggggct gcgtaggaac ggcgagacat gttactccgc agaatcagtg atatggtcgt
     1141 tgaatcgagg ggccagggta cggctatcga taaaaataaa tccccgctcc gtgttcgcct
     1201 ggtaggttaa gttaatctcg gtattggtcc cgcgccaatt atactgctgc gtgaggcccc
     1261 gcatcatctg cccggggacg cgctcaagaa aaccgaactg ttcttccagg tgggtgagga
     1321 tttgtttatg acggtcgttg ccctggtagc ggatggtcac acgggcgaat tgttcatcga
     1381 ccgataagaa gcgaaggctt tcgactggga ttccgtcaaa caccggcagg gcgtctctca
     1441 attggtattc gttgatgtgt ggacctgccc gtgcgacttc caagtccggt ctcgtggtca
     1501 gggcagtgcc ccaagccagg ctctgaaaac catttggatc gttcaccatc ggcaccgcat
     1561 gggcgagaag ggggcagccc atacccaaca agaatcccag taggacgagg tatcggttcg
     1621 cgtgatggct ctgccgtccg atcatgatga gaacgctacc atggtcgatc ctatggcgca
     1681 actccctcag ttttgttgag aatgccttga ggcatagctt ataatgcgcg ggtttttcct
     1741 ggtctgttgc agcggggaac cgacacgatg attgaaagcg acgcattcgt ccaagcatta
     1801 caggatatcg gggtggattt ctttacggga gtcccggatt cgatcctggg aggtatcatc
     1861 gccgagttga tggagcggca gctctatacc ccggccgttc gggaagatga agcggtcgcc
     1921 atggctgctg gggcctatat gtccggtaag gtccctgccg tgctcatgca gaactccgga
     1981 ctgggtacgt cccttaacgc cctcatttcg ctcaacatga tctactgtca gccctgtatt
     2041 ctcattgtgt cttggcgcgg ctatcaaggg aaggatgccc cggagcatgt ggtcatgggc
     2101 gaagtgatgc ctgagtttct cgatacgatg aagattcccc atcgcaccct gtcggaaaag
     2161 acggcaattg acgatctgaa atggctggct gagacgttta tgaaacagcg catccccgtc
     2221 gcccttttga tgacgaaagg tgttgtaaga gggttacacc catgaggccc gagcaaggaa
     2281 cgatgcaaag tcgggccatg gccatggcgg ccttgttgga actcctcacc gatcagccgg
     2341 tgattatttg caacgggttt ccctcccgtg aggcctacaa gattgccgat cgtcccaccc
     2401 atttctatat gatcgggtcc atgggaaatg cgccggccat tgcgcttggt gtggcgctgg
     2461 ccaagccgaa caagcaggtg gtcgtgttcg acggggacgg taacgtgttg atgggcatgg
     2521 ggacgttggc gacggtcggc gccttgaaac caaagaattt tatccacgtg gtcttcgata
     2581 acgaagtgta cggcacaacg ggtaaccaac cgacgatctc caatgtggtg ccacttgaga
     2641 aggtggcgaa ggcagccggg tatgtgaacg tcgaacgggt gcgggaacgg gaagatctcg
     2701 tctacgagtt taaagacatg ctgaagaaag acgggccgag catgttgctg gtgaaagtca
     2761 atgaaatggt ggaagatgtc gggcgtgtgt tgcttgatcc gccggatatt accaagcggt
     2821 tcatgaaagc gatagaataa ggaggattga tcagactgtc ctgtgtgctc gcagagcacg
     2881 cacgctcaga aggtggtcgc ttgggaaaag agcgcgtctc ggcgcgctcg gggttgggtg
     2941 ggtgaaaaag ttgcgcgcag tgaagggcaa tccgattatt cattggtgag tgggggacga
     3001 ttcgcaagat atggttctct tgaatccagg accggtgaat gtctcggagc gggtgcgaca
     3061 ggcactgttg catccggatg tctgccatcg ggagtcggaa tttaccgaac tcttgcacgg
     3121 gattcaggca aagctgctga agctcttcgt gccgggggct gaggcagatt atgctgccgt
     3181 agtgttgacc gggtctggaa ctgcggcggt ggagtccgcg gttatgtcgt cgctgccgca
     3241 cggcaaacgg atgttggtgc tcaacaacgg cgtgtatgga gagcgcattt cacagatgat
     3301 cggcctttat cgcctgggtg tgtccgaact caaatatgat tggacgacca ggccggatcc
     3361 tgaacggttg cgactcgcgc tacgacagca tccggaagca cacgctgtcg caatggtcca
     3421 ccatgaaacg accaccgggc tcatcaatcc ggtgaaggaa atcgcggatg tcgtcgacag
     3481 ccagaaccgg gtgttcattc tcgacgcggt cagtgccttg gcaggggagc agctggatat
     3541 cgccgggtcc cacatctata tggtagctgg tacagccgga aaatgtattc aaggattccc
     3601 aggcgtgtcc tttgtgctcg tcaacaaggg atttctggaa cgaatgcgta catatgccag
     3661 acggtcgatc tatcttcaca tgactcaata tgtggatgat gagggccgcg ggacgattcc
     3721 gttcacaccg gctgtgcagg tttactacgc ctttgacgaa gcgttgaacg aactcatgga
     3781 ggaagggatc gccaagcgaa ttcaacggta caggaaggtc gcgaccctca tccgagaccg
     3841 tatggcgaaa ctcggcgtga agccgctcct gactccggat aagcagtcca gcacgatcac
     3901 cgcctatttt ctccctgagg gcatgaccta tcaggtgttg cacgatcggc tgaaagaaga
     3961 gggttacgtg atctatgcgg gccaaggcca actccagagc aaaattttcc gtgtggcgaa
     4021 catgggtgcg ctcacagagg cgcagttcgt cgggtttctc gatgcatttg aacggatttg
     4081 cggcacggca tgaaagcgat tatcctcgca gccggggttg ggaagcgcct gtggcaggtc
     4141 acgcaacatc gtcccaagtg cctcatcgag atcggcgggc agaccctctt gcatcgctac
     4201 ctcacgtcgc ttcggagcgt cggcgttcag tctgccgaca tggttgtggg atacaagcag
     4261 gagatgattc gagcggctgt gacagcggac tcttgtggag tccgcgtaaa tttcctcgtc
     4321 aacgagcagt tccaccgcgg cagtatctcc tctctctgga tcgcgcggga ggcgttggat
     4381 gatgacgcga tcgtcatgga tgccgatgtg ctcttccatc aggaaatctt gcgtcgtttg
     4441 gtacaatcac cctatgagaa cgcattgctt atggatgaca cggtcaagca gacaggggaa
     4501 gagtgcatgg tcgtggtcga aggcgggcgc gtgattgcgc tgaccaagaa gatgccattg
     4561 cgctatgact atgcgggaga aggcgtgggg tttctcaagg tccggcacgc cgatacgccg
     4621 tatgtggtgg cgtcgctcaa gacccatgtg gaccacgagg cctggcagat ggaatatgag
     4681 gatgctctgt tgggattttt tcgtgacgtg aaagtcgggc atgaaaagat cggtggactc
     4741 ccctggaccg aaatcgattt tatcgaggac gtgtcgaagg cggaacggga tgtcttgccg
     4801 aaactataaa agcggagggg agaaggataa cggggtaaac gggataccag cttttaggat
     4861 gccggggtga gggggagagc ccttcactcc gaagtcaacg atcggccgat catgagtgag
     4921 agcgtagttc agaaacagac gggtctccaa ggattgagca cggcgattct cttgccgtcg
     4981 gtgagcctgt tcggcgaacc tgccggattg tataccgatg aggtgggacc gctgactagc
     5041 gtcgtaggca tcggcctgtt tcagcgggcg gtcttgacgc tgcagcgagc cggtattcga
     5101 caactgatgg tgctggtcgg gccagaggag tctcagctca ggcaggcctt ggccaaaggg
     5161 ccacgtgtga cgatccccgt gcgctggatg ccgattcgtg agttcccact ggatgaccct
     5221 cgcacctggg aagcgctggc tgctgaagtt cgtggcttct gtctcctgtc cggggtgcag
     5281 gccgtgtttg cgagcccctt gatcgagagc ctacgccagt cggtacagga ggggcaagcg
     5341 atcgtagtgg cgcgggccga tagtgggcgg gatcgacagc cacgccggtc gaatttgcgc
     5401 gtgaaggtgc agtcgggccg gctgctctcg atcacatcga gacaggatga caccgcgctg
     5461 gtggtcacag atttggtggt gcttccggcc agtttcatga gcgtggtcgg tcaagccagc
     5521 ggggaagtcg gcacggtgcc gattcgtcga tggctcgaac gggcagccgt ggatgggcat
     5581 gtgcgggtgt tgtccacgga aacgaaccgg tcccactggt atcaggacgt gcacgatgcc
     5641 gccgatgtga aggccgctga gaaaaagctc ttcaattccc tcaagggcga attcgaaggc
     5701 ttcgtcgacc ggttcttcaa tcggaaggtc tcccgctggt tcacgcgcat ctttctcgcc
     5761 gcaggctttt cgcccaatgc cattacgatc gtggccagcc tgatcgggtt ggccgcggcc
     5821 gcaggttttg gtctcgggac ctacagcgcc gggattattg ctgcactgtt gttccaactt
     5881 gcggcgatta tcgattgctg cgatggtgaa gtagccaggc tgacgttcac cgaatcacca
     5941 ttcggggcct ggctcgatat tgccatggac aatgtcgtcc acatggcgat ctttgccggc
     6001 atagccgtgg gctcctacct gcatctggct gggagcgacg gggcttgggt ttctctcgcc
     6061 ctcggcgcgg cagcggttct ggggaatgga ctctcgttct ggctcgtcac cagggcacaa
     6121 aagatcaaag ctgcgagtgg atggaagacg ccggtgcagg ctgcctggtc ggacttcatg
     6181 ctcaagaatg tcgcgagccg agacttttcc gttgtcgtgc tgatctttgc ggtgctgggc
     6241 aagctcgact ggttcctctg gatggccgcg atcggatccg tggtgttctc gatcctgatg
     6301 ttgtgggtca tcaggccttc cgccacgtcc cgtgcttaag ggcttcctcc tcgttgtcgg
     6361 ccttctcacc ctcggattcc tcgtctggca tatcggtccc gagcgtatct acgatgcggc
     6421 atcgcaactc gggccggtcg cgctcctcat tctgctgatt ccctccacga tcatgtatgt
     6481 cgttgaagcg tacggttgga aaatcacgtt gggtccatcg gcgaagcata ttccattctg
     6541 gcgtgtgctc gcgattcgga ctgccgggga agtggtgaac atgaccacgc cgaccgccta
     6601 tgttgggggc gaacccgtca aggcctatct gctcaataga catcatgtgc caatggtgaa
     6661 ggggcttgcc tcagtcgtca ttgcgaaaac gacaatgacg attgctgaag tggtcttcat
     6721 cctcttgggt cttgcgctcg cattctggag agtcggcgct gacggctcat cagggcagac
     6781 cgttgcagcg gcgctggtca gtgtcggcct gctggtactc gtgaccgcgg cgttggtgtt
     6841 cgtacagcag caaggactct tcacgtggct attgggagtt cagaggaagc ttgggctgaa
     6901 gatccactac ctcgagaagc gtgagaagaa actcctgtcg ctggatcgaa cgatcttgga
     6961 tttttaccgc
//
LOCUS       DATIPF010000279         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1467473, whole genome shotgun sequence.
ACCESSION   DATIPF010000279 DATIPF010000000
VERSION     DATIPF010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467473"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..858
                     /locus_tag="VLL06_06975"
     CDS             226..858
                     /locus_tag="VLL06_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HSS30743.1"
                     /translation="MAQSQSSNGTIAGVVTILGLGLVWVTVATNFPSEEELSSAELPA
                     ETRLAAVSSSSPSVPPTLDLTIPTSPPLPSEAAMVRPGDERSTDSHVDAPVVARLAPS
                     AVQATRLKCEANMEQLCPDSLDGAARTRCLKQRAKLLPPMCQSQLQERFVKWKEDRSR
                     LIAACREDVKRFCTVVKPGGGEILQCLQSHDQEVSDRCYQALPKDTFFFK"
     gene            complement(989..1693)
                     /locus_tag="VLL06_06980"
     CDS             complement(989..1693)
                     /locus_tag="VLL06_06980"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HSS30744.1"
                     /translation="MPETLPQPAKLLVKLHGKGSQHIELTLDTVTIGRKTDNVLVIED
                     PAVSGHHARIVKIQAVFFLEDLKSTNGTALNGRPITRHQLRDADVITIGQHRLVFQEN
                     VAPSTSAPAPFVDLDQTTVLRGTDRTPDGPTLSAKVLIIAGKTDRVEYPLTKQVNVIG
                     SQDGAVIRLTGWFAPKSAATIARRGQSYSISPSRDAKYLLVNGTDVAGQQELKDGDQI
                     EVAGVKLTFCILSGTK"
     gene            complement(1728..>2384)
                     /locus_tag="VLL06_06985"
     CDS             complement(1728..>2384)
                     /locus_tag="VLL06_06985"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HSS30745.1"
                     /translation="EAICTHLAEAAQRADLPLIGSCDATVSPTANRLASAIRAANDVI
                     HRESWSRPDYVGMGTTVVAALLHDEVLAIAHVGDSRLYLIRNGAIQSLTTDHSWVTEQ
                     MLKGFITEEEAERSPRRNIVTRALGVENSVDVELTEVPVKSGDMLLLCSDGLTRGVHP
                     NDILHILGGSDSLPVMSNRLIAMANEAGGDDNTTVVIVALRDDLQAGLWERLKKLFAA
                     "
BASE COUNT          447 a          661 c          708 g          568 t
ORIGIN      
        1 actcgctcta tccgggtatg tgtgtgctcg gctcggcaca ttctcaatgg gtttccgcga
       61 atacgcctcg ggggttttca ggaccagcgg tcgcgcctct gccaggcctc atccgcctca
      121 tcatacaccg caccgtctgg cagacatctg gtagaaaccg gacaattcgt cgattttttt
      181 gacggcttat cctatgctgg gtctggtcga atatagggag cagtcatggc tcagtcacag
      241 tcttcgaacg gtaccattgc cggcgtcgtc accattcttg gccttggact cgtgtgggtg
      301 acggtggcca ccaactttcc ctcggaagaa gaattgtctt cagccgagct gcctgctgag
      361 actcgtttgg cggccgtgtc atcgtcttct ccgtccgtgc cacccacgct ggatttaacc
      421 atccccactt ctcctccgct gccttcagaa gcagccatgg tgaggcctgg agatgaacga
      481 tcgaccgact cacatgttga cgctccggtt gtcgcgcggt tggccccttc ggcggtgcag
      541 gcgactaggc tgaagtgcga agcgaatatg gaacaattgt gtcccgactc tctggatggt
      601 gctgcccgca cgcgctgttt gaagcagcgt gccaaactgc ttccgccgat gtgccaaagc
      661 cagttgcaag agcgattcgt caagtggaag gaggaccgga gtcgattgat cgcggcgtgc
      721 cgtgaggatg tcaaacggtt ctgcaccgta gtcaaacctg ggggcggaga gatcctccag
      781 tgcctacaat cgcatgacca ggaggtttcg gaccgatgtt accaggctct tccgaaggac
      841 acgttcttct tcaaatagtc gggtgcgtat cagcgagagg cgctctggca cgatactgaa
      901 tagtccgtcg gcgcgtagac tgtgctgtgt gattggctag gcgggcaggg tgcaattcga
      961 agtgtgggcc tgtcaaattt tccgcaacct acttggttcc tgagaggata cagaacgtca
     1021 gcttcacacc tgctacttca atctggtccc catctttgag ttcctgttgt cccgcaacat
     1081 ccgtcccgtt cacgagcaga tacttcgcat cacgagacgg actgatggaa tagctctggc
     1141 ctcgtcgagc gatcgtcgcc gcagattttg gcgcaaacca gccggtcaac cgaatgaccg
     1201 ctccgtcttg cgacccaatg acattcacct gtttggtgag cgggtattcc acccgatcgg
     1261 ttttcccagc aatgatcagg accttggctg agagcgtcgg tccgtccggc gtgcggtccg
     1321 ttcctctaag cactgtggtt tgatccagat cgacaaatgg ggcaggtgcc gacgtgctag
     1381 gggccacatt ctcttggaac acgagccggt gctgtccgat ggtgatgaca tccgcgtcac
     1441 gcagttggtg gcgggtgatt ggccggccat tgagggcggt gccgttggtg ctcttcaaat
     1501 cttcgagaaa gaagacagcc tggattttca cgatccgcgc atgatgccct gagacggcgg
     1561 gatcttcgat cacgaggaca ttgtcggtct tccgaccgat cgtcacggtg tcaagcgtca
     1621 gttcgatatg ctgcgagcct ttcccgtgaa gtttgacgag cagtttggct ggttgtggca
     1681 gagtttccgg catggcgttc cctcctgtgc taggctcctg tgattcgtta cgcggcaaat
     1741 aattttttta gccgctccca cagtccggct tggaggtcat ctcgcagggc cacgatgacc
     1801 acggtggtgt tgtcgtcgcc gccagcttcg tttgccatgg cgatgagacg attggacatg
     1861 acggggaggc tgtccgatcc gccgaggatg tgaagaatgt cgttcgggtg gacaccacgg
     1921 gtgagtccat ctgagcaaag caacagcatg tctccactct tgaccggtac ctccgtcagt
     1981 tccacatcga cgctgttctc gacgccgagg gccctcgtca cgatgtttct gcggggcgag
     2041 cgctcggctt cttcttctgt gatgaatccc ttgagcattt gttcggtgac ccaggaatgg
     2101 tctgtggtga gggattggat ggccccgttt cgaatgagat agagccggct gtctccgaca
     2161 tgggcaatgg cgagcacctc atcatggagc agtgcggcga ctacggtggt acccattcca
     2221 acatagtcag gcctgctcca tgactcgcga tggatgacat cattcgcggc acgaatcgcg
     2281 ctggcgagac gattggccgt aggagatacc gtggcatcac aggaaccgat cagcggcagg
     2341 tctgcgcgtt gggctgcttc ggccagatgt gtgcaaattg cctc
//
LOCUS       DATIPF010000280         5623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_226349, whole genome shotgun sequence.
ACCESSION   DATIPF010000280 DATIPF010000000
VERSION     DATIPF010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226349"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VLL06_06990"
     CDS             complement(<1..852)
                     /locus_tag="VLL06_06990"
                     /inference="COORDINATES: protein motif:HMM:NF040057.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HSS30746.1"
                     /translation="MAVDFRRFGADMAGIATRLGMCLVGWLMLWMVLPLTGGAAGNGG
                     SSEATIASTGGVPLDITAERIDYLQEQEIYEADGSVVVKQGALRLTADHMTIQSLPGV
                     LIATGHVRLTDPKADLATERLELNVNTEAGVVTHGRVYLKATNTLVEGRLLQRFSEDH
                     YRVKEGRFTNCDAQEGETPAWRFTFKDLDMNAGDNVAFKGGWLCVNDVPMIPIPTLTY
                     PLSKRHSGFLIPNVGYDNRFGMRLQESFFWVINPSQDLTISPIYYSGLGYGSDFQYRY
                     VLDRQSKG"
     gene            complement(821..2116)
                     /locus_tag="VLL06_06995"
     CDS             complement(821..2116)
                     /locus_tag="VLL06_06995"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HSS30747.1"
                     /translation="MTYSTAVAYLYRLQKHGIKLGLETMTALMVRLGMPQILYRTLHI
                     AGTNGKGSTAAMAAAMLQAAGYRVGLYTSPHLVEFRERIRVNGEMITESQVAQLTEQL
                     QAFCQPDLSPTFFEYTTAMALQHFADSGVDVAVLEVGLGGRFDATNVVTPTACAITTI
                     SLDHQEYLGTTLSSIAFEKAGIVKPRVPVVVGRLDDDAWATIEQVARERQVPVFRLDK
                     DFRTEGKTPQQFSYRGLGRQYDGLTCALEGRHQLDNAACALALLGAAASQGITVEAEA
                     VRAGLRTVHWAGRLEVVDRRPTILLDGAHNPAAARALADYLTCSDRSFRSHPIVLVLG
                     MMRDKDHRGFVEPLKCLVDAVVLTQADLPRAATALELQASLEGLLPHPHVVPSPGEAM
                     ALAKQLATPDGLVCVTGSLMLVGECKAWFRGCGLSPLRG"
     gene            complement(2334..3170)
                     /gene="accD"
                     /locus_tag="VLL06_07000"
     CDS             complement(2334..3170)
                     /gene="accD"
                     /locus_tag="VLL06_07000"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HSS30748.1"
                     /translation="MAWFKKQKSTEPEPPKRSKISEGMWLKCNHCREIVYRKEVERNN
                     KVCPKCDYHFPISVMERIALLVDLGTFKEWDAELSPQDPLNFQDTKSYKDRVKAHQGK
                     TGRKDALVIGEGMMNGRRVVLCVFDFSFMGGSMGSVVGEKLCRAIDRALETRLPVILV
                     TTSGGARMQEGILSLMQMAKTSAAVAKLGEAKLPFICLLADPTFGGVTASVAMLGDVI
                     LAEPKALIGFAGPRVIEQTIKQQLPDQFQRAEFLLEHGMLDMIVERKQLKETLSTLVA
                     HF"
     gene            complement(3309..4016)
                     /locus_tag="VLL06_07005"
     CDS             complement(3309..4016)
                     /locus_tag="VLL06_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30749.1"
                     /translation="MLDTDFLTILGLGLVLGLRHALDTDHLAAVSTVLAQRPSLRACG
                     MIGFSWGLGHTVVLLLVGAVVLVLHMPIPEPIALAAEFGVGTMLVFLGGILGLRLIRE
                     RWHVHTHDHDGEQHVHLHSHALVEDHGHGHWWSDSIRPFCIGMAHGLAGSAALLLIVL
                     SSAQSVSAGLMYIAVFGLGSIVGMMLVGIAVSLPVLWSVTLGRPVFLAVQGLASLGSV
                     AIGLTMMVNIALGGQPI"
     gene            complement(4067..5131)
                     /gene="moaA"
                     /locus_tag="VLL06_07010"
     CDS             complement(4067..5131)
                     /gene="moaA"
                     /locus_tag="VLL06_07010"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HSS30750.1"
                     /translation="MDHAIVGDSVVPVGDMFGRPLRSLRLSVTDRCNLRCKYCMPEED
                     YVWLPREDVLTHEEMAGLTGYFTDLGVDRVRLTGGEPLLRRNLSQLIRLLLQNRRLTD
                     IALTTNGILLADQAEALYEAGLHRVTVSLDTLRPERFHQLTRRDEYARVMEGIESVVR
                     AGFTGLKLDTVVIRGFNDDELVALIEFGKQVQAEVRFIEYMDVGGANDWSQPQVLSRA
                     AMLTQLSEHYGVIEPMPERGAAPAQRYLLPDGTIFGIIPSTTTPFCGTCDRSRVTSDG
                     MWYHCLYATAGTDLRTPLRAGASADEMRALLRAGWEGRRDRGAEERKALERDGLRTGG
                     LIGIEKLRENPHLEMHARGG"
     gene            complement(5183..>5623)
                     /locus_tag="VLL06_07015"
     CDS             complement(5183..>5623)
                     /locus_tag="VLL06_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HSS30751.1"
                     /translation="DPASDDSQFVRVQREDFSVDAEISRVRGRSKRIGGISIFLGTAR
                     DRSKGRDVASITFEHYEGMAQKKLREIRERALKDFDIIEVAILHRYGDIGIGDNIVLI
                     VVGAEHRAEAFRACKWAIDELKHITPIWKLEHTPEGEVWVEEHP"
BASE COUNT         1283 a         1778 c         1448 g         1114 t
ORIGIN      
        1 ccccttcgac tgccggtcca atacataccg atattggaaa tcgctcccat agccgaggcc
       61 gctgtaataa atgggagaga ttgtcaggtc ctggctagga ttgatgaccc aaaagaagct
      121 ttcttggaga cgcatgccga aacgattgtc gtacccgaca ttggggatga gaaacccact
      181 gtgccgcttg gagagaggat aggtcaacgt aggaatcgga atcatcggga catcattcac
      241 acagagccat cctcccttaa atgccacatt gtcaccggca ttcatgtcaa gatccttgaa
      301 cgtaaatcgc caggccggcg tctcaccttc ctgcgcatcg cagttcgtaa accggccctc
      361 cttcacccga taatggtctt ctgagaaccg ctgcagaagg cgcccctcca ccaaagtatt
      421 tgtggccttg agatacaccc gtccgtgcgt gaccacgcca gcctcggtat tcacattgag
      481 ttccaaccgt tcggtcgcga gatcggcttt gggatcggtg agtcgaacat ggccggtcgc
      541 gatcagtaca ccgggcaacg actggatggt catatgatca gccgtcaagc gcagggcacc
      601 ctgtttgacc accactgagc catcagcttc gtagatttct tgttcttgga ggtaatcgat
      661 tcgttccgca gtgatatcga gcggaacccc accggtagat gcgatcgtcg cttcggaact
      721 tcccccattc cctgcagcac cccctgtgag tggaagtacc atccacagca tcagccaacc
      781 gaccagacac atccccagcc gcgtcgcgat gcctgccatg tcagccccga agcggcgaaa
      841 gtccacagcc acggaaccat gccttgcact cccccacaag catgagcgat cctgtgacac
      901 agaccaaacc gtccggtgtc gccagttgtt tggccagcgc catcgcctca ccgggagatg
      961 gcacgacatg cgggtgagga agtagacctt cgagggacgc ttggagttcc agagcggttg
     1021 ccgcgcgggg gaggtccgcc tgtgtcaaca ccaccgcatc aacaaggcac ttcagcggtt
     1081 cgacaaagcc tcgatggtct ttgtcgcgca tcattccaag aacaaggacg atagggtggg
     1141 atcggaaaga ccggtcagaa cacgtgaggt agtcggccaa ggccctcgcg gcagcaggat
     1201 tgtgcgcgcc gtccaataga atcgtcggac ggcgatcaac cacttccaat cgtccggccc
     1261 agtggaccgt gcgcaagcca gctcgcaccg cctcggcctc gaccgtgatc ccttgagatg
     1321 ccgccgcccc taacagcgcg agggcacagg ccgcgttatc aagttgatgg cgaccctcca
     1381 gggcacatgt cagtccgtca tattgcctac ccaaaccgcg ataggaaaat tgctgaggag
     1441 tctttccctc tgtgcgaaaa tccttgtcga gacgaaacac tgggacctgc ctctcccttg
     1501 ccacttgttc gattgtcgcc cacgcatcat cgtcaagcct ccctaccacc acgggaaccc
     1561 tcggtttcac gatcccggcc ttttcgaacg cgatcgatga gagtgtggtt cccaaatact
     1621 cctgatgatc gagagaaatt gtcgtgatcg cacaggccgt gggcgtgacc acgttcgtgg
     1681 catcgaatct tccacccaat ccgacctcca gcaccgccac atcgaccccc gaatcggcaa
     1741 agtgctgaag cgccatcgcc gtcgtatatt cgaagaacgt cggtgacagg tccggctggc
     1801 agaaggcctg aagctgttcg gtcaactgtg ccacctgcga ttcagtaatc atctcgccat
     1861 tcacgcggat gcgctctcga aactccacga gatggggcga ggtatagagc cctactcgat
     1921 accctgccgc ctgcaacatg gcggcggcca tggccgcggt agatcccttc ccattcgttc
     1981 cagcgatgtg cagagtacga tacaggatct gtggcatccc gagtcgcacc atcaatgccg
     2041 tcatcgtctc aaggcccagt ttgatgccgt gcttttgcag acgatacaga taggccacag
     2101 cagtggaata cgtcatgggt aaccaggcgg gaagagcact agccagaatt ctttatgaat
     2161 ctcatggcga ggttatcaac actgcggtct accgaaacgt gtcgcatgcg aagacggcag
     2221 aggctggcgt ctcgtgggcc atcgggaagc ccacacatgc acacgtccct gggatgaaag
     2281 gatcgactgc catcggggtt gtagacacta tagacattca tagcggatcc ggcttaaaaa
     2341 tgggcaacga gcgtgctcag tgtttctttg agctgtttgc gctccacgat catgtcgagc
     2401 atgccatgct ccaacagaaa ttcggcccgc tgaaactgat ccggcagctg ctgtttaatg
     2461 gtctgctcga tcacgcgggg gccggcaaac ccgatcagcg ctttcggttc ggccagaatc
     2521 acatcgccca gcatggcgac gctggcggtc acgccgccga atgtcgggtc ggctagcagg
     2581 catataaatg gaagcttggc ctcgcccagt ttggcgacag cggcagacgt cttggccatc
     2641 tgcatcagag aaagaatccc ctcttgcatt cgggctcctc cggacgtcgt gaccaaaatg
     2701 actggtaacc tcgtctccag tgcgcgatcg atcgcccgac acaacttttc cccgacgacc
     2761 gatcccatgc tgccgcccat aaagctgaag tcgaacacac agagcaccac acgacgaccg
     2821 ttcatcatcc cttcgccgat caccagggca tctttgcggc cagtcttccc ctgatgcgcc
     2881 ttgacgcgat ccttatacga tttcgtgtcc tgaaagttca gcgggtcctg agggcttaat
     2941 tccgcatccc actctttgaa cgtcccgaga tctaccaaca gcgcaatgcg ctccatgaca
     3001 gaaataggga aatggtaatc gcacttcgga cagaccttat tattgcgctc gacttctttg
     3061 cgatagacaa tctcccggca gtgattgcat ttcagccaca tcccttccga aatcttggat
     3121 cgtttcggag gttcaggctc ggtcgacttc tgctttttaa accaggccat tgtgcgctcc
     3181 tctcagtccg acaccggacc aacgcacctc acgtgtaaga tgctgaatgg tccgccagca
     3241 gcggcagaat accatagccg ccctcatgac gcacgtagat ttcccattga gaaaagagcg
     3301 ttcgaacgtc agatgggttg cccgcccaag gcgatgttga ccatcatcgt cagtccgatg
     3361 gccacgcttc caagacttgc cagcccctga accgcgagaa agacgggacg cccgagagtc
     3421 acagaccaca agacaggcag gctcacggcg atcccgacca gcatcatccc cacgatcgag
     3481 ccaagtccga agacagcgat atacatcaac cccgccgaca ccgattgtgc agaggacagc
     3541 acgatcaata acagtgccgc cgacccggcc agaccatggg ccatgccgat acagaagggg
     3601 cgaatggagt cactccacca gtgtccatgt ccatgatcct caaccagcgc atggctatgc
     3661 agatgcacgt gttgttcacc gtcatggtcg tgcgtatgca catgccagcg ttctcggatt
     3721 aaacgcaacc cgagtatgcc tccgaggaag accagcatcg taccaacacc gaactcggcc
     3781 gctaacgcga tgggttcagg aatcggcatg tggagcacca acaccactgc cccgaccaac
     3841 aacaacacca ccgtatgtcc gagcccccag ctgaatccaa tcataccgca ggcacggagc
     3901 gaaggccgtt gcgcaagtac agtggagaca gcagccagat ggtccgtgtc caacgcgtga
     3961 cgaagcccca gcaccaaccc aagtcctaat atggtaagga aatctgtatc caacatgcgg
     4021 gcatcaacac gaattggccc tgtgacgtcg gcggcaagat gggacgtcag ccgccccgag
     4081 cgtgcatttc aagatgcgga ttttcacgaa gtttctcgat accgatgagc cccccggtac
     4141 gcaacccatc ccgttcaagc gccttgcgct cttcggcgcc tcggtctcgt cggccttccc
     4201 agccggcccg gagcaaggcc cgcatctcat ccgcagacgc accagcgcgg aggggcgttc
     4261 gcagatccgt ccccgccgtg gcgtacagac agtgatacca cataccgtcg gaggtgacgc
     4321 gactacgatc gcacgtgcca cagaacggtg tggtcgttga aggaataatc ccgaatatcg
     4381 tcccatccgg caacagatat cgctgagccg gcgccgctcc ccgctcaggc atcggctcaa
     4441 tgaccccata gtgttcgctc aactgcgtga gcatggcggc tcgggacagc acttgtggtt
     4501 gagaccagtc gtttgcacca ccgacgtcca tatattcgat aaaccgaacc tctgcctgta
     4561 cctgcttgcc aaactcaatc agtgcgacca actcatcgtc attgaaacca cgaatcacca
     4621 ccgtatccag tttcaagccg gtgaatcccg cgcgaaccac cgactcaatt ccttccatca
     4681 cgcgggcata ctcatcacga cgggtcagtt ggtgaaatcg ttccggcctg agcgtatcga
     4741 gactcaccgt gacacggtgg agccctgctt catacagcgc ctcagcctga tccgccagaa
     4801 gaatcccatt cgtcgtcagc gcaatatccg tcaatcgccg gttttgaagt aataagcgaa
     4861 tcaactgcga cagattgcgt cgaagcaacg gctctccacc ggtcagccgc acgcgatcca
     4921 cgccaaggtc ggtaaaatag cccgtcaggc ctgccatttc ttcatgggtc agcacgtcct
     4981 cgcgcggcag ccagacgtaa tcctcctccg gcatacaata cttgcagcgg agattgcatc
     5041 gatccgtcac ggacaaacgt aagctgcgaa gcggccggcc aaacatatcg ccgactggca
     5101 cgacagaatc gccgactatt gcgtgatcca acagcgtcct cctcgcctta gaacccgatg
     5161 cacgccgtgc gtgtcacatc attcatgggt gttcctccac ccacacttcg ccttccggcg
     5221 tgtgttcgag tttccagatc ggcgtgatgt gtttcaattc gtcgatcgcc cacttgcagg
     5281 cacggaacgc ctcggccctg tgctcagccc ccacgacaat cagcacgatg ttgtcaccga
     5341 tgccgatgtc accataccgg tggaggatcg cgacttcgat gatgtcaaaa tccttcaacg
     5401 cccgctcgcg aatctcccga agcttcttct gcgccatccc ttcatagtgc tcgaaggtga
     5461 tactggcgac atcacgccct ttggagcgat cacgcgccgt cccgagaaat atcgagatcc
     5521 cgccgatacg cttcgatcgg cctcgcaccc gactgatctc cgcatcgacg gagaaatctt
     5581 cccgttgcac acggacaaat tgggaatcgt cactggccgg gtc
//
LOCUS       DATIPF010000281         7302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_585619, whole genome shotgun sequence.
ACCESSION   DATIPF010000281 DATIPF010000000
VERSION     DATIPF010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7302
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585619"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VLL06_07020"
     CDS             <1..624
                     /locus_tag="VLL06_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HSS30752.1"
                     /translation="RPLASGELSPAHAFLVLGCFLSLAGMLVLFLDPLTMLISPIAIL
                     LAGLYPFAKRVIHIPQAMLGIAFGWGTIMAWTASRGTIEAPAWCLFAATVCWAIGYDT
                     IYALQDREDDRRIGVKSSALLFGSLTWIAVGTVFCAMLLLLGFAGWLAHIGWIYYGAL
                     AAIGLWCLRQALQLRQTVPTPTAFHMFQQHVWVGAAVFIGMVAGFLL"
     gene            complement(627..1655)
                     /locus_tag="VLL06_07025"
     CDS             complement(627..1655)
                     /locus_tag="VLL06_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSS30753.1"
                     /translation="MKAAPLYVSGVVGALIGIGVLVAGGCANEQQKKGHELYGHYCSH
                     CHGESGRQNEGYNWSSMPDPKPKDLTNKSEMSTLKDKDIFDTIYRDMKDTGEGGDEIG
                     DDEFGVPTMPTFKYTLSEDEIWAIVGYVRGLHGTKLEFNVEERKKQLATALQTAQATL
                     EQATKTYEEAEKKASEEAEKKNVDVDDAAYANELAAMAKAKKELDAAQAAMNNFTARP
                     GKGQSVARPDLTVKPDQVPKLVELGKRYYEDKYGCNGCHAIGGEGGKVGPALDRAGFR
                     LNPTWVYRWMKNPQAMNAETRMPALGLSDADAKAVTMYLSTLRAPANPEGSTTKPEEP
                     LPVSKPAS"
     gene            complement(1652..2518)
                     /locus_tag="VLL06_07030"
     CDS             complement(1652..2518)
                     /locus_tag="VLL06_07030"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HSS30754.1"
                     /translation="MKRGLTQIFPNAIAGVGLVLGLLIVQPVFAEEKAPASAPPEAAS
                     ESNAVSAPLVKGALPADDPTAAIWNTAPQARFPMSPQVHWPNRILDATVKDLSVRALH
                     DGTNVAILLEYADPTEDADDSAAVEFMVGDKKAHFAHGQPMAQVEGGPVNIWFWRNKD
                     AKGVDMNAKGFGTLKVQEHQDVKAKGEYSNGTWKVVFTRPLATGHPEEDTQIEMGGFI
                     NIAFAVWDGKKDISGAVKEKGSQKAVSSWWNFRAEAPPNYTGYYYAAIAVGLALGFQF
                     VLIRNLKKGNRA"
     gene            complement(2663..3382)
                     /locus_tag="VLL06_07035"
     CDS             complement(2663..3382)
                     /locus_tag="VLL06_07035"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSS30755.1"
                     /translation="MKGSLVTPVIVIVVVYLICKFIVPIIPGSAPLPSSVVILYMMLT
                     ISGIGIFYTLSKESKDAFWGPMERFLTGENIGGMQALRYGVLILFPLLVGWQTYSSTA
                     VSDQPPGENRTIHPAPPGEYAGLSNPVAKTPDAIQQGKGFYAAYCSPCHGGNFDGKGP
                     AARGFNPPPANFADPTTIAMLQESYLFWRIKKGGVGLPIEGMPWKSAMPRWEVELPDE
                     WIWKIIMGEYDGAHQSPRTWE"
     gene            complement(3393..4019)
                     /locus_tag="VLL06_07040"
     CDS             complement(3393..4019)
                     /locus_tag="VLL06_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSS30756.1"
                     /translation="MSEVVRLQLIGLSVVGVGILALLFIRGTFIRVVGFVSIVLGLFT
                     LVSLSIPQMASLPPVEEKFDIATVKTPTDLATIGQKVFFSKGQCALCHTIGPSESARC
                     PDLKGIGAKLSREFLFESLTQPQAYIYQDYRVEGMAKEYPATMPYINKNPIGLSRNEI
                     LAVIAFLQQMSGEPISIAVSELAAPEPAPAAPVQKAAESGPVAVAQVH"
     gene            complement(4085..5950)
                     /locus_tag="VLL06_07045"
     CDS             complement(4085..5950)
                     /locus_tag="VLL06_07045"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HSS30757.1"
                     /translation="MQHQGGWIQRLLQTGRDHGGTLSMLAIAAAFLSLPMLAFGADAT
                     PPAPTEYRDVPYVGARNVIWVVAQLHLLLAGFVLGVPLFAWVCEIVGWKGGEKRYDKL
                     AKEFTKLLTSAYSTTALFGGILLFLLIGFYPKLMNYLSDIFFPSFIVYCILFLLETVT
                     LYLYWYGWDTMQDGGKKTFHIFLGFMLNFFAFFIMIIPNSWATFQASPVVLAEGSDIA
                     RAWAATWNPTWWPVNIHRLIANVVLGGYICGAYAGIRYLSAKTPEERSHYDWMGYVGN
                     FIGVFGLLSLPFAGYRLMREIYQYNQQMGITLMGGFLSWLFILQAMLIGVLFLGSNYY
                     FWIGMTYRIPGSDVKYKKLMLAMLVALLVCLGIWMTPHSLVASMEEARKMGGSHHPLL
                     GVFGVMSAKMTVANLMILVTFMSFIMYWRAGKQETVGWAKVAKAFMGLVLALSSIAVI
                     VLGVWGYFVPAIIRINYFSTSQVLVVLGVLLTITPLTSVLLKSAKTTTEMVWGVMPPR
                     AGYSLVLNAIMIILLMTLMGYARSSSRVHWHVYGVLRDTSQYAFSPALGYASGIMALC
                     TFLFCMIVAFIFWVATMGDKAKAPSVSASKAELPHGLPAMAGASNALDKPNTRKF"
     gene            complement(5976..6980)
                     /locus_tag="VLL06_07050"
     CDS             complement(5976..6980)
                     /locus_tag="VLL06_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30758.1"
                     /translation="MNDHLSPTKLGFGILWPACWTGLPFKLALAVLFLAFGIVDFESR
                     IALAFLMLLASPVTVFALPTITMGLGSHIGEGAGIGLLFMLAIPIDIWALGLTGRTFF
                     LERCRREPPARLGLTLWWKCAFVGAIFLPLLWVAERATTHVSISAAHSIFEDGFLEGT
                     PIAERISIELTLWGTVATVVLSCLFVIGLSLVGRMLRGTIQAAPHAAENYEGLITRWD
                     LMRVPADPGLMLTALSCVAVVLSLLFWSSLPVLTPHPHECCKKAEVRAVPEFKPLETL
                     NKDEKMISQLAARVEFLIEQQAAAELEKEKSKKKGGADHEAVKEPDATGPVPAAVMAP
                     "
BASE COUNT         1623 a         2173 c         1920 g         1586 t
ORIGIN      
        1 cgtccgctcg catcgggtga actcagcccc gcccatgcct tcctcgtcct cggttgcttc
       61 ctttcactgg ccggaatgct cgtgctattc cttgacccgc tcaccatgct catcagtccg
      121 atcgccatcc tgttggccgg cctctacccc tttgcgaaac gagtcatcca catcccacaa
      181 gccatgttgg gtatcgcctt cggctggggt actatcatgg cctggaccgc ctcgcgggga
      241 accattgaag cgccggcctg gtgcctattc gccgccacgg tctgctgggc aatcggatat
      301 gacacgattt acgcgcttca agatcgtgag gacgatcgtc ggatcggcgt gaaatcctcg
      361 gcgttgctat tcggttcgtt gacgtggatc gccgtcggga cggtcttctg cgcgatgctg
      421 ctgctgctgg gattcgcagg ctggcttgcc cacatcggct ggatatacta tggcgcgctc
      481 gcggccattg gtctatggtg tctgaggcaa gccctgcagc tcaggcagac cgtaccgact
      541 cccactgcct tccacatgtt tcagcaacat gtgtgggtag gagccgccgt cttcattggg
      601 atggtcgcag gatttctact ctgaggtcag ctcgccggct tgctgacggg cagaggctcc
      661 tcgggtttag ttgtcgaacc ctctggattt gcaggtgcac gtaacgtgct caggtacatc
      721 gtcacggcct tggcatcggc atcgctgagc ccgagcgccg gcatcctggt ttccgcattc
      781 atcgcctgcg ggttcttcat ccaccgatag acccatgtcg gattcaatct gaatcctgca
      841 cggtcaagcg ccggtccgac cttgccgccc tcaccaccga tggcgtggca gccattgcag
      901 ccatacttgt cttcgtaata ccgcttgccg agctccacca acttcggaac ctggtccggt
      961 ttcacggtga gatccggcct ggctacactc tggccttttc ccggtctggc cgtgaaattg
     1021 ttcattgcag cctgtgccgc atcgagttct tttttcgcct tcgccatggc tgccagttca
     1081 ttggcatagg ccgcatcatc gacatcgaca tttttctttt cggcctcttc gctggccttc
     1141 ttttcagcct cttcataggt cttcgtggcc tgctctaatg tagcttgcgc cgtttggagc
     1201 gccgtcgcca actgcttctt ccgctcctcg acattaaact ccaacttcgt gccatggaga
     1261 ccgcggacat acccgacaat cgcccatatc tcgtcttcag acagggtgta cttaaatgtc
     1321 ggcatggtgg gcacgccgaa ttcatcgtca ccgatctcgt cgcccccttc acccgtatcc
     1381 ttcatgtcgc ggtaaatcgt atcgaagatg tctttgtctt taagtgtgct catctctgac
     1441 ttgttcgtga gatctttggg cttcggatcg ggcatcgagg accaattgta tccttcgttc
     1501 tggcgtccac tctcaccgtg acagtgcgag caatagtggc cgtacagttc atgccctttc
     1561 ttctgctgct cattggcaca gcccccggca accaagaccc caatgccgat cagtgcaccc
     1621 accactcccg atacgtatag gggggccgcc ttcatgctcg attccctttc ttgagattgc
     1681 ggatcaggac gaattggaat ccgagcgcca ggcccacggc gatggccgca tagtagtagc
     1741 cggtataatt cggtggcgcc tctgcacgga aattccacca ggaggagact gccttttgag
     1801 agcccttctc cttcaccgct ccgctgatgt ccttcttgcc gtcccagacg gcaaaggcaa
     1861 tattgataaa cccgcccatt tcaatctgcg tatcttcttc cgggtgtccg gtagcgagtg
     1921 gccgcgtaaa gacgactttc caggtgccat tgctatactc gcctttggcc ttcacgtctt
     1981 ggtgctcttg caccttgagt gtgccgaagc ccttggcatt catgtcgaca cctttggcgt
     2041 ctttgttcct ccaaaaccag atattgacgg gtccgccttc cacctgcgcc atcggctggc
     2101 catgggcaaa gtgggctttc ttatcaccca ccataaactc aactgcggct gaatcgtctg
     2161 catcttccgt tggatcggcg tactccaaga gaatggccac attggttcca tcgtgcaagg
     2221 ctcgcacgct cagatctttg accgtggcat ccagaatacg attcggccaa tggacttgag
     2281 gcgacatcgg aaaccttgcc tggggtgccg tgttccaaat cgccgcagtc gggtcatcgg
     2341 ccggcagagc acccttcacc aatggagcgc tgaccgcatt gctttccgag gctgcttcgg
     2401 gtggcgcact ggccggagct ttttcttcgg caaagacggg ctgcacaatc aacaatccca
     2461 gaacaagtcc gacacccgca atggcattcg gaaagatttg ggttagtcca cgtttcatac
     2521 gtttcgtcct ctctctactc acttctcagc gggtggggcc atctgccttc cacgatgcat
     2581 acaaaatagg gcaccgtttt ctcgtggacg ttctgcgagc aaatatgagc cctttagcga
     2641 catagacgga tcttccgctc tgctattccc acgtcctcgg tgattgatgg gctccatcgt
     2701 actcgcccat gatgatcttc cagatccatt cgtccggtag ttccacttcc caacgtggca
     2761 tcgcggattt ccatggcatc ccttcgatcg gaaggccgac gccgcctttc ttaatgcgcc
     2821 agaagagata gctttcctgg agcatggcga tcgtcgtggg atcggcgaag ttggccgggg
     2881 gaggattgaa tcccctggct gctggcccct tgccgtcaaa gtttccacca tggcaggggg
     2941 aacaatatgc cgcatagaag cccttcccct gctggattgc gtctggtgtc tttgcaacgg
     3001 gattcgacaa gccggcatat tcgcctggcg gggccggatg aatcgtccgg ttttctccgg
     3061 gaggctgatc gctgaccgcc gtgctgctat aggtttgcca gccgacgagc agcgggaaca
     3121 gaatcaagac gccgtaacgc aacgcctgca tcccgccgat attctctccc gtcagaaacc
     3181 gttccatcgg tccccagaaa gcatctttgg attctttgct cagtgtgtag aaaatcccaa
     3241 tccccgatat cgtcaacatc atatagagaa tcacgacact ggacggcagc ggcgccgagc
     3301 cagggataat ggggacaata aacttgcaga tgagatagac gaccacaatt acaatgaccg
     3361 gcgtgaccaa tgaacccttc atacgttcct cctcagtgca cttgggccac ggctaccggg
     3421 ccagactcgg ctgccttctg caccggagcg gcgggggccg gttccggcgc tgccagttca
     3481 gataccgcga tggaaatcgg ctccccactc atctgctgca agaaggcaat gaccgccaga
     3541 atctcattcc tggacagccc gatgggattc ttattgatgt acggcatggt ggccggatac
     3601 tccttcgcca taccttcaac gcgatagtct tgatagatgt aggcctgcgg ctgcgtcaag
     3661 ctttcaaaaa gaaactcgcg gctcagtttg gccccgattc ccttcaagtc cgggcaacgg
     3721 gccgattcac tgggaccaat ggtgtgacac agggcacact gacccttact aaaaaagact
     3781 ttttgcccaa tggtcgccag atcggtcggc gttttgaccg tggcaatgtc gaacttctct
     3841 tcaaccgggg gtaaggatgc catttgcgga atcgacaatg atacgagggt gaagagaccc
     3901 aggacaatcg agacaaatcc cacgactcga atgaacgtac cgcgaatgaa gaggagggcc
     3961 agaattccca cccccaccac gctgagaccg atcaactgta atctgacaac ttcgctcata
     4021 tatccccttc gggtagttag aagtgcctgg tcctgagggc tgcgttaccg tccaaattca
     4081 cctttcaaaa cttgcgcgta ttcggtttat ccagcgcatt cgacgcgccc gccatcgccg
     4141 gcaggccgtg cggcaactct gccttgcttg ccgagacact cggcgctttc gccttatcgc
     4201 ccatggtggc cacccagaag atgaaggcga caatcataca gaataagaat gtgcacaagg
     4261 ccatgattcc agatgcatag ccgagcgccg gtgaaaacgc atattgcgac gtgtcgcgaa
     4321 gcacaccgta aacatgccaa tggacgcgtg aggacgaccg tgcatagccc atcagcgtca
     4381 tcagaaggat gatcatgatc gcgttgagca cgagggaata gccggcacga ggaggcatca
     4441 ccccccagac catttccgtc gtggtcttgg cgctcttcag caacacgctc gtcagcggag
     4501 tgatcgtcaa caagacaccg agcacaacca acacctgcga tgtcgaaaaa taattgatgc
     4561 ggatgatcgc aggaacgaaa tatccccaca cgccgaggac gatcacggcg atcgaagaga
     4621 gggcaagaac gagacccatg aaggcttttg caaccttcgc ccagcccacc gtttcttgct
     4681 tgccggcccg ccaatacatg atgaagctca tgaacgtgac gaggatcatg agattcgcga
     4741 cggtcatttt agccgacatc acgccgaaga caccaagaag tggatggtgg ctccccccca
     4801 tctttctcgc ttcttccatg ctggccacaa gtgagtgggg cgtcatccag atgcccagac
     4861 acaccaatag agcgactaac atcgccaaca tcagcttctt atatttcaca tcggagccgg
     4921 ggatgcggta ggtcatcccg atccagaaat aatagttcga tccgagaaag agcacgccga
     4981 tgagcatggc ctgcaaaatg aacagccatg acagaaagcc ccccatgagc gtgataccca
     5041 tctgctggtt gtattgatag atttcacgca tgagcctgta gccggcaaag gggagggaca
     5101 acagaccaaa gacgccgatg aagttcccga catagcccat ccagtcatag tggctgcgtt
     5161 cttccggggt cttggcggac aaatagcgga tcccagcata ggcgccgcag atgtacccgc
     5221 cgagcaccac gttggcaatg aggcggtgaa tattcacggg ccaccaggtc ggattccatg
     5281 tcgcggccca ggctcgcgca atatccgatc cctcggcaag caccacggga ctcgcttgga
     5341 acgtcgccca ggagttcggg atgatcatga tgaagaacgc aaaaaagttg agcatgaagc
     5401 cgaggaaaat atgaaaggtc ttcttgccgc cgtcttgcat cgtatcccag ccataccaat
     5461 agagatacaa cgtcacggtc tcgagcagga acaggatgca atagacgata aacgagggga
     5521 agaaaatgtc gctcaagtag ttcatcaact tgggatagaa cccgatcagt aagaacagca
     5581 gaatgccgcc aaacaatgcc gtcgtggaat aggcggaagt caggagcttg gtgaactcct
     5641 tggcgagttt gtcatagcgc ttttcgccgc ccttccagcc gacgatttcg cacacccagg
     5701 cgaacagtgg cacacccagc acaaatcccg cgagcagcaa atggagctgc gcgacaaccc
     5761 aaatcacgtt acgagcgccg acgtatggaa catcacgata ttcggtcgga gccggcggag
     5821 tggcatctgc gccaaaggcc agcatgggaa ggctgaggaa ggccgcagcg atcgcgagca
     5881 tcgagagcgt ccctccatga tccctgccgg tctggagaag ccgctgaatc catccaccct
     5941 gatgttgcat tgccctcacc tcatcatctc ccacgttatg gtgccatgac ggcagccgga
     6001 accggccctg ttgcgtccgg ttctttcacg gcttcgtgat ctgccccgcc ctttttctta
     6061 ctcttttcct tctccagctc ggcggccgcc tgctgttcta tcaaaaactc aacccgcgcc
     6121 gcaagctgag aaatcatctt ttcgtcttta ttcagcgtct caagtggttt gaattctggt
     6181 accgccctca cttccgcctt cttgcaacat tcgtgcggat gtggtgtgag caccggaagg
     6241 gaagaccaga ataggagcga taatacgacg gccacacagg ataatgctgt cagcatcaat
     6301 cctggatctg ccggcacccg catcaaatcc cagcgggtaa tcagcccttc ataattctct
     6361 gcggcatgcg gtgctgcctg tatcgtgcca cggagcatcc gtcccacaag cgagagacca
     6421 atgacgaata aacatgaaag cactacggtc gccacagtgc cccagagagt cagttcaatg
     6481 ctgattcgct ccgcaattgg agttccctcc aggaaaccat cttcaaatat cgaatgagcg
     6541 gcagagatcg agacatgcgt ggtcgcacgt tcagccaccc agaggagcgg cagaaagatg
     6601 gcgccgacga atgcacactt ccaccaaaga gtcagcccca atcgagccgg cggctcgcgc
     6661 cgacaccgtt ccagaaaaaa tgtccgcccc gtgagcccga gcgcccagat gtcgatcgga
     6721 atggccagca tgaacagcag gcctatgccc gcgccttcac cgatgtgtga gcccaatccc
     6781 atcgtgattg ttggcagggc gaacaccgtg accggactcg ccaggagcat caaaaacgcg
     6841 agggcgattc tggattcaaa atccacgatt ccgaatgcca gaaagagcac ggcgagggca
     6901 agcttgaatg gcaagccggt ccagcaggcg ggccagagaa taccgaaccc aagctttgtg
     6961 ggggacaggt gatcgttcat ccagataccc ggcagtgcct gtcaaacgat gcacgatgaa
     7021 ctacagcacg tgcgaatgac ctcggcgttg tgcatccttt gcacgtcacg gacagcagag
     7081 atgcaccggc aggatgctca aaaagactgc tcagcaaggc cgcagcgagc gaggaggcga
     7141 ggcgtacgct tcggtacgtt gagcctctga gagcctctga acgaagtgag aacgaagctg
     7201 acggactttt tcagcatcct gcctaatcca aaaaatctcg attaataatg gcagacaccg
     7261 tgaacaggag gatcgaaacc gtgagcacca cccatccgat aa
//
LOCUS       DATIPF010000282         5627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1205442, whole genome shotgun sequence.
ACCESSION   DATIPF010000282 DATIPF010000000
VERSION     DATIPF010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205442"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VLL06_07055"
     CDS             <1..217
                     /locus_tag="VLL06_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSS30759.1"
                     /translation="WGAPAADREPAADSLTPREREVLQWLAGGKTDRDIGDILGISPR
                     TVHKHLEHIYRRLEVTGRRQAVAVAFS"
     gene            complement(234..935)
                     /locus_tag="VLL06_07060"
     CDS             complement(234..935)
                     /locus_tag="VLL06_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSS30760.1"
                     /translation="MANQTYESPSHAATSNASVARVDMKLEIVVISVSDIDRAKEFYG
                     GKLGWRLDADYDNGKDFRVIQFTPPGSGCSVIFGKNVTAAAPCSTQGLYLIVSDLEAA
                     RKQLLGRGIQISQVFHDAGGVYAGTDEPYLFGQIRVSGPDPEHRSYRSFASFQDPDGN
                     GWLFQEITTRLAGRIDSTTTSFASMNDLASAFRRAEAAHGQHEKRMGGQRDENWPDWY
                     AAYMVAEQAGAELPT"
     gene            1216..1680
                     /locus_tag="VLL06_07065"
                     /pseudo
     CDS             1216..1680
                     /locus_tag="VLL06_07065"
                     /inference="COORDINATES: protein motif:HMM:NF022458.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
     gene            1722..2876
                     /locus_tag="VLL06_07070"
     CDS             1722..2876
                     /locus_tag="VLL06_07070"
                     /inference="COORDINATES: protein motif:HMM:NF023352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3443 family protein"
                     /protein_id="HSS30761.1"
                     /translation="MVLLGCGNSGSSGPSGPSGSNVLPITVNPAAGALCRFANALCTH
                     VTICQPSTSNCQTIADVLVDTGSSGLRIFASTLTITLPSSTMGECLYFGSGTDWGRVQ
                     SADVVLGGEPAVFVPIHVIDPTFAGQYTSGGQPASNVCGVGGVDSSPSEVGFNGILGV
                     GLFPSDGGFYYNCASLPCTAVAVAPSQQIQNPVGLLPVDNNGVIVTLPSVPASGSPAV
                     TGSLILGIGTASNNQPSGVTVLPTNALGHIVTHFHPSPGVSTQNVGIIDSGSSVLFFS
                     DASLSIPQCPPPLPPDPDLSFLYCPAAPLNFAAVNTELSGVPSSTVSFQIVDPRPLLG
                     SGNGAIGNIGGPAFAPGVFDWGLPFFFGRTVYVGLSGRTSVLGTGPFWAY"
     gene            complement(2944..3195)
                     /locus_tag="VLL06_07075"
     CDS             complement(2944..3195)
                     /locus_tag="VLL06_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30762.1"
                     /translation="MIVTRPRQHVVTGTYYIGKDAGITKMSWQAARFRSYAEAKQFAA
                     ENRIALNGSTYISIKNSTDLAFQNQELFNNSGQITRNTS"
     gene            complement(5000..>5627)
                     /locus_tag="VLL06_07080"
     CDS             complement(5000..>5627)
                     /locus_tag="VLL06_07080"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HSS30763.1"
                     /translation="LDQEPWDFERTPPTKYPLLLYFHPLTIYRYQVIKQADLVLVMLL
                     LGHEFSSDLKRRNFEFYDPITTGDSSLSPCIQSIMAAEVGDMGKALEYARVATLMDLG
                     DVAGNVKDGCHIAAMGGVWMVFVYGFAGLRDYHGELHFRPTLPDVLDCLRFELMFQGQ
                     ILEVLIGQETTRYTLKTGNGLQLRHDEKEILLSPEVPTFDCPNRKPSE"
BASE COUNT         1192 a         1603 c         1535 g         1297 t
ORIGIN      
        1 gtggggcgcg cccgccgcgg accgcgagcc cgccgcggat tcgctcacgc cgcgcgaacg
       61 cgaggtcctg caatggctcg ccggcggcaa gacggatcgc gacatcggcg acatcctggg
      121 catcagcccg cgcaccgtcc acaagcacct cgagcacatc taccgcaggc tggaggtcac
      181 cggccgccgg caggccgtgg cggtggcctt ctcctgactc cctgagccgg cgttcaggtc
      241 ggcagctccg cgccggcctg ctcggccacc atgtatgcgg cgtaccagtc gggccagttc
      301 tcgtcgcgct gcccccccat gcgcttttcg tgctgtccat gggcagcttc cgcacgccga
      361 aacgcgctcg ccagatcgtt catcgatgca aagctagtcg tcgtggagtc gatgcgaccg
      421 gccagccgcg ttgtgatctc ctggaacagc cagccgttgc catccggatc ctgaaacgag
      481 gcgaacgagc ggtagctgcg atgctcggga tccggcccac tgacccggat ctgcccaaac
      541 aggtagggtt cgtccgtgcc ggcgtataca ccgccggcgt cgtggaacac ctggctgatc
      601 tggatgccgc gaccaagcag ctgtttgcgg gcagcctcga ggtcggagac gatcaagtac
      661 aggccctggg tggaacaggg agccgccgcg gtgacgttct tgccgaagat gaccgagcac
      721 ccggagccgg ggggtgtaaa ctggatcacg cggaaatctt taccattgtc gtagtcggcg
      781 tcgaggcgcc acccgagctt cccaccgtag aactctttgg cacggtcgat atccgagacg
      841 gagatgacaa cgatctcgag cttcatgtcg actctcgcga cgcttgcatt gctggttgcg
      901 gcgtgactgg ggctttcata cgtttgattt gccatgttct cctcctatgg gttcacctag
      961 atcgaaagtt agattataga tgcgagcatt ggaaaagctg catgcactcg caaagggggg
     1021 aagaattatc agtgaaatac cattaaaagg caagatggta ttgttgtgcc acgtgaagcg
     1081 gcattggcat tgagcatagc tatcgtatgc atgttcgcct acgacaacgg cgcgtctctt
     1141 gagccagtcc acaagcacac gtttgttccg agtctgcagt ataatgttct attacttttc
     1201 tgccagaggc atgtgatgaa acatcagcga gctcttgagc tggcgattgc attcggtttg
     1261 atcctgatcc tcgatctccc aaaaacctgg gctgtgttgg gagaaccagc gagttctgtg
     1321 gacacggatc gcgtggcgct tgccggacag gtgagcataa ccacagcacc gctctatacg
     1381 atccaggaga tcaccacacc ggagctcgtt gtgcgggaat atgtgtcgag agatatggtc
     1441 tttgctgtgg cctggacagg acgaagacct ccaaacttag tctcgctgtt tggttcctat
     1501 tttcaggagt aacaggaggc agcgaccgta gcatcaaatg ggcctaggat gcgcgggatg
     1561 acccacctcc aggggcccca tgtcgtggta gaaacaggag gtcatccggg agatatacgg
     1621 ggaagagcct acctaccttc actcctccct tcgggcgtga caaaagagat gatccaatga
     1681 ttcggaaaag gtgcaaatca gttcttttcg ggatggcttt catggtgctt ctgggttgtg
     1741 gaaattctgg aagctcaggc ccctccgggc cttcggggtc caatgtcctc ccgatcaccg
     1801 tcaatccagc tgctggcgcc ctctgcagat ttgccaatgc actctgcact cacgtcacca
     1861 tctgccagcc cagtacttca aactgtcaaa ccattgccga tgttcttgtg gataccggct
     1921 cgtcggggct caggattttt gcttcgacac tgaccatcac actcccatcg agcaccatgg
     1981 gggaatgcct ctattttgga agcggcactg actgggggcg tgttcaatcc gcggacgtcg
     2041 tgttgggtgg ggagccagcg gtattcgttc cgatccatgt gatcgatccc accttcgcgg
     2101 ggcaatacac ctccgggggc caacccgctt cgaacgtctg cggcgtgggg ggcgtcgact
     2161 cgagtccttc agaagtcgga ttcaatggca tcttgggcgt cgggttattt ccgtccgacg
     2221 gtgggtttta ctacaactgt gcatcgcttc cttgtacggc tgtcgcagtg gctccctctc
     2281 aacagatcca gaatccggta ggacttctac cagtggataa caacggggtc atcgtgacct
     2341 tgccgtctgt gcccgccagc ggatcccctg ccgtgaccgg ttctctcatc ctcggcatcg
     2401 gaacagcatc gaataaccaa ccttccgggg tcacggtgtt gccgaccaat gcattgggcc
     2461 acatcgtgac acactttcat ccgagcccgg gcgtcagcac ccagaatgtg gggatcatcg
     2521 attctggctc cagtgtcctg tttttcagtg acgcgtcgtt gtcgattccg cagtgtcccc
     2581 cacctctccc accagatcca gatttatctt ttctgtactg tccggctgct ccattaaact
     2641 tcgcagcggt caacacggag ttgtcagggg ttccctcaag cacagtcagt ttccagatcg
     2701 tcgatcccag gcccctgctg ggatcaggga atggagccat cggcaatata ggcgggccag
     2761 catttgctcc gggggtgttt gactgggggc tgccattctt ttttggcagg accgtctatg
     2821 tcggcctgag tggacgaaca tccgtgctgg gcacagggcc attttgggcg tattagagga
     2881 cggtctcaga cgacctcggc tggatcggtc tatcttgtcg cgcagccgcc tgattcgttg
     2941 tcatcatgat gtgttcctcg tgatctggcc tgagttattg aacagctcct gattctgaaa
     3001 tgctaagtct gtactgttct ttatgctgat gtaggtcgat ccattcaacg cgatgcggtt
     3061 ctctgcggca aattgttttg cctcggcata gctgcggaat ctggccgctt gccaactcat
     3121 cttggtgatg ccggcatcct tgccaatata gtatgtcccc gtcacgacat gctgccgcgg
     3181 tcgagtcacg atcacaaaac acatctcctc tgtcggcaac atcccttccc ccctgccgtt
     3241 gaagtcgcgt gcctcagcgc accctctcta cgccgccaga ctcgatggcg tcctcctctg
     3301 ccttgtcgcc gaagtaaggg gcgcaccctg tgggcggaga gtaatctggt aggccgaaca
     3361 accgaggtcg agcaatgaaa cccgtccgtc ccgcgacagt cgatccacgg cgaggaacac
     3421 ctctccccaa ctgaaatggg gaagggatgt agtaacatcg tcgaggtggc agggaccgcg
     3481 tctctgcagc gtttcaacca cagcctcttc tatgagtctc atggcgcaga acctccgggt
     3541 gaagtgaagt gctcgtgtgc gcacacatca tgacagcaag atgtcacgtg ggaattgggt
     3601 tataacctgg tatgggcaga ggtgattacc tgacattcaa tcatctggag tcgtcaacca
     3661 aggcactcgc agggatgctg cgcggctagt cgctcggcgg tagtctatca aggtcggaac
     3721 ttctcagagg ggttctgaac aatggcgcag gggtcattgc tagggcggag atattgtctt
     3781 acacggcttg tgccttcctc agtagtcgtg cgtctcaagt cgcttcagag tgcgcatgcc
     3841 tgtttttacc agacgcacac attcggcgaa tatttgggaa aagctatctg gcttactctc
     3901 gcctttcgag aagggcgggg ataagtcgat gtaaataatc gcttccccgc attccctgca
     3961 gtgataggtg cccatcagta caccatcatc actgcggttc aagcgaacca attcttgaat
     4021 cggatgctca cagaggggtt cgctcgggat agttccattt tttactgcct cgctcacttg
     4081 cggcctgccc aatacttcgt tctctccagc ccagtccgaa ggcgctcatt gatcctcccg
     4141 cattcgaaat ccgcgacgat tccccgcact tgaacgaggc gcttcgtggc tgggacgggg
     4201 gctgtggtgt cgaggtgttg ctgttgggga tacagcccaa tatattcgct gtggagttcg
     4261 ttgaagaaga ttacgacctc ctccaagttg cgcccagaca accaacagat caagcagcta
     4321 ccatgtcaca ctcctctcgc tccatgcatg gcaaaggcgg tcaaaaccgg agcccaattc
     4381 atttgcgctt ctgatctcaa catcagctta acagtggaac tgcgcgcagc cgactaggaa
     4441 atcccccagc atgacagcat ctggtttctg tccaaaaggc acacgaccag ctcagcttct
     4501 ttcccacatc aaggtgctga tttatcccac acgcgatccc ggcgcacgca acacccatgg
     4561 tgagggctca tcgcgtcagg tgttcatgtt ttcccacagg caaccctaaa taaagattgc
     4621 gcaatacttc gtggcggaat ggggctccac ttactttcga gcggggcagt gaaaacctca
     4681 ctaccttctg ccatatggtc cgagaaccag atgacctcaa tacgtcgttg gagtgctgcc
     4741 gcgcagttcg attgcaattc tgtccggtta gataagtatg ggaaccctga ggataactgg
     4801 atggtcttat aatcgtacag gacccgaatc ctcaccaaat ccccttttct gcgaatagta
     4861 cttcgatcaa catacacatc atatccacct tcactctggg cagaggaaat ccacacccac
     4921 tccgcatagg tgggtccagt attcagcacg aggagcaaga atagaaagag tccttgtctc
     4981 atcatcagcg agagcgctct cattccgagg gtttccgatt tggacaatcg aaagttggca
     5041 cttcgggtga caaaagaatt tctttctcat catgacgcag ctggagtcca ttgccagttt
     5101 tcaatgtata tcgagtagtt tcctgcccaa taaggacttc gagaatctgc ccttgaaaca
     5161 tgagctcaaa tcggaggcag tccaacacgt ccggtaaggt tggtcgaaaa tgcagttcac
     5221 catgataatc gcgcaggccc gcaaacccat agacgaatac catccagact cctcccatcg
     5281 cggcaatatg acacccgtct ttgacgttcc ctgccacatc gcccaaatcc atcagggtcg
     5341 ccaccctggc atactctaac gcttttccca tgtcgccgac ctcggcggcc ataatgcttt
     5401 gaatgcacgg agagagggag gagtccccgg tggtgatggg gtcataaaat tcaaagttgc
     5461 gtcgtttcag atccgatgaa aactcatgac cgagcagcag catgacaagc acgagatcgg
     5521 cttgcttgat gacttgatac cgatagatcg tcaacggatg aaagtagagc agcagcggat
     5581 actttgtggg cggggtccgt tcgaaatccc aaggttcttg gtccaga
//
LOCUS       DATIPF010000283         6053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_938470, whole genome shotgun sequence.
ACCESSION   DATIPF010000283 DATIPF010000000
VERSION     DATIPF010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938470"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VLL06_07085"
     CDS             complement(<1..1059)
                     /locus_tag="VLL06_07085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSS30764.1"
                     /translation="MTQADEKSLAGRWSPAMLLMLAGALGLLARAWTILHTERQGYAV
                     DDLFDALAWNLATTGSFSMDGMTPSAHAGPLYPAILAAFYAIVGHRPEWVVYVHVGFD
                     IITGLCIYLVGARLFHPWIGALAAAALFLYPAYWTYDPRIRSESLLTCLLSAWLWAMV
                     VCRSSARMFSFVGAGVLAGLTILCKPVTILLAIALIGLACLGPDSVRAKTVRAGLYLL
                     ACLLVVLPWTMRNYRAFDQVIPVSAGVGAGLWMGSDPLSRGSWPMPEAMERQIWESAG
                     ITPLPYAHAMYDVSTDRLLRAKGWARIREEPVRYLWLSLTRAFDFWIGNSFYLVNSGQ
                     GFGRGLTADASVRGWLIAS"
     gene            complement(1056..1796)
                     /locus_tag="VLL06_07090"
     CDS             complement(1056..1796)
                     /locus_tag="VLL06_07090"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSS30765.1"
                     /translation="MERRDAYPTDTQHIVNLSGYVWAARQVPHLDRLRVLDLACGTGY
                     GSDYLSGKAGRVVGVDCAPQIVARNRARYGHSGASFLAMDGCALGFLDESFDCILSQD
                     TIEHIMDDRRFVAEVARVLRKTGTLVIFTPHGKGVAYKPEDPYHVREYNQEEFHGILS
                     PYFSTIRWFGRRQGARLKAAERSMDAVRQFDPGGIRNLVPRPIRHWLGGQVSRLQGGP
                     SLKAITPEDIEYEEGVASDTNLIGICMK"
     gene            complement(1813..2808)
                     /locus_tag="VLL06_07095"
     CDS             complement(1813..2808)
                     /locus_tag="VLL06_07095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSS30766.1"
                     /translation="MTPTDTQDSTLPAIDLSIALVSYNTKDILLDCVRSVRAHTTAIA
                     YEVIVVDNYSRDGTVPAIKKIYPDMMIIANPDNRGFAKAVNQALAVSRGRHVLLLNSD
                     TIVRDQALATMVAHLDDHPDIGAVGCKQWTGDGYLNQTCFPFPSIRDHLFYSALFQRV
                     APTMQAAAAAMHAVDCTQSQDVDWANGACLMVRRSLLLELGGLDEDFFMYFEDVDLCR
                     RLRQQGYRVRHLAEAEIIHFIGRSSGRDHERLQLVWEFSRIRYIEKHFSPIKRRVMKG
                     WIAAGTGWRLMQSIWCAPAAQRRRRMQSCLTTMRRLWSGPQSVEQILAYSSGRQR"
     gene            complement(2821..3564)
                     /locus_tag="VLL06_07100"
     CDS             complement(2821..3564)
                     /locus_tag="VLL06_07100"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSS30767.1"
                     /translation="MTKTMMQGVQDWWNQRYPEWVPEIALRYLPVVESICAEPSRGLI
                     LEVGVNRSGLTTYLRRPVIGVDCVMSAERVPLVTAVLAKGQALPFQSGTFDFTVCMDT
                     LEHIECSDRNHFLRELIRVTTRRVYIGCPMGRQSEREDHALQQYYRAQNGKSFPFLDE
                     HIANGLPRLEAVLAEIEGIAKESGRPMRLHCEPNLNVWVHRILLRLWMRTDRVSYVLH
                     RLAVALVHVRRWLNAGPCYRQIIVAEFDA"
     gene            complement(3568..5064)
                     /locus_tag="VLL06_07105"
     CDS             complement(3568..5064)
                     /locus_tag="VLL06_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HSS30768.1"
                     /translation="MITSRDMVLEETQVKMDRAEPAPPRLRHRILQAGGWAGVGFVLD
                     KVIAAVQLMVVARLLVPADFGVMAASATIVLAFMTISELGLESALIAKAKVDREDLAV
                     AWTVAIVRGVAMAACLWAAAEVIGQAMQMPQLASLLRVHAWLLVLQGLQSPAMAIVLK
                     NLNLRRRVTMDLIRRVIEATAIIILALWLRSVWALLLGQMVGMCIGSLLSYWVAPFRP
                     RLSLYRPACDYFLRYGKQLNVATLCIFGVMSGGELVIGRVLGQEALGFYQIALAIPLL
                     LGVRVTVLIHQISMPTYALLQQDRPGVARVFELQMGLVGLIFIPLAASVAVLAPVIVP
                     LAFGPQWLAIVDPLRMLCVYVVFAGYSSVMTALHYGANRPDLQMKSWGAQFLFYAMVI
                     VPLTMGFGLTGAAAALVASYAVGVLLQSMGTRSLMGTAVNATFWSAGRSALLAGFLAG
                     IILLMSETTANLPTGWVLVSTCLGALGLYGWYLWSMEVPRLKVLWEHR"
     gene            complement(5061..>6053)
                     /locus_tag="VLL06_07110"
     CDS             complement(5061..>6053)
                     /locus_tag="VLL06_07110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSS30769.1"
                     /translation="AGLRPFSFLIASWHVLRQELAAWRPEVLLWFDSPGQIAPLLALS
                     DHACATVLFVNGLPEEEVQGVWRLPFVRWILRAALKKAACRVQAVVSICREIVAQMEN
                     AWGIPPESCHVIRNGVDEDRFHPQPHDAARTALGLSVDGPYIGFVGGFFPWHGLETLI
                     DAVPFVHAKVPSATFLLVGEGQTKFALEQLVQQQGLAERVRFVGRADWARVPTWIAAC
                     DVCVVLNRQTRSYPGDSMKLWEYLSCGRPVVTTIGPGYGETVEAIGCGLAAKPGDASD
                     VARQLVRLLEDPVERMKMGERGRMAVIQAHTWDARAKELEQVCSAAHAARQRTT"
BASE COUNT         1464 a         1908 c         1579 g         1102 t
ORIGIN      
        1 gctggctatc agccagcctc ggacggaagc atccgccgtg agtccccgtc caaatccctg
       61 acccgagttg accagataga acgagttccc gatccagaaa tcgaatgctc gtgtcaggga
      121 gagccagaga tatctgaccg gctcttctct aatacgcgcc cagcccttcg cacgcaagag
      181 ccgatcggtg gacacgtcgt acatcgcatg ggcatagggc aacggtgtaa tcccggcact
      241 ttcccaaatc tgccgttcca tggcctccgg cataggccaa ctcccacgag acagcggatc
      301 gctccccatc cagagaccgg caccgactcc ggcagacaca ggaatgacct ggtcgaacgc
      361 tcgatagtta cgcatcgtcc acggcaacac cacaagcaga caggcaagca gatacaggcc
      421 tgcacgaacg gtctttgcac gaacagagtc tggcccgagg caggcaagac caatcagcgc
      481 aatggctaac aggatggtca ccggcttgca cagaatagtg aggccggcca gcacaccggc
      541 ccccacaaag gagaacatcc gtgccgagga acggcatacc accatggccc acaaccaggc
      601 actgagaaga caggtcaaca gactctcgct ccggattcga ggatcgtagg tccaataggc
      661 ggggtagaga aacagcgccg cagccgcgag tgcgccgatc caaggatgaa agagcctcgc
      721 ccccaccagg tagatgcaga ggccggtgat aatgtcaaaa ccaacatgta catacaccac
      781 ccactcaggc ctgtggccaa ctatcgcgta gaatgcggcc aggatcgctg gatagagggg
      841 accggcatgg gccgacggcg tcatcccgtc catgctaaag gagccggtcg ttgccagatt
      901 ccaggccaac gcatcgaaga gatcatcgac ggcatacccc tgtcgttccg tatggagaat
      961 ggtccaggct cgtgccagca gcccaagcgc accggccaac atcagcagca tggcgggaga
     1021 ccatcgcccg gctagactct tctcgtcagc ctgcgtcatt tcatgcaaat cccaatgaga
     1081 ttcgtatcgc tggccacacc ttcctcatat tcaatatctt ctggagtaat cgccttgagc
     1141 gatggccctc cttgaagccg actgacctgg ccgccaagcc aatgccgaat cgggcgtgga
     1201 acgagattgc gaatgccacc aggatcaaac tgccgcacgg catccatcga ccgctctgcc
     1261 gctttcaatc gggcgccctg acggcgaccg aaccagcgaa tggtcgagaa gtagggggac
     1321 aggatgccgt gaaactcctc ctggttgtac tcccggacgt gataggggtc ttcaggctta
     1381 tatgccacac ccttcccgtg aggtgtaaaa atgacgagcg tgccggtctt gcgcaagacg
     1441 cgtgcgactt cggcgacaaa ccgtcgatcg tccataatat gttcgatcgt atcttgcgac
     1501 agaatacagt cgaaggattc gtccagaaac ccaagcgcac aaccatccat ggccaaaaaa
     1561 gacgcccccg aatgaccgta acgggcacgg ttgcgggcga cgatctgtgg tgcgcaatca
     1621 accccgacga ctcgaccggc cttcccgctt agataatcgg atccataccc cgtcccgcag
     1681 gcaagatcga gcactcgcaa ccggtcaagg tgtgggacct gcctggctgc ccacacatat
     1741 cccgacaaat tcacgatgtg ttgtgtgtcg gtcggatagg cgtcgcggcg ttccatgaga
     1801 tggtccttct ccctaccttt gccgtcctga tgaataggca agtatctgct cgaccgattg
     1861 cggcccactc cacaatcggc gcatggtcgt cagacaactc tgcatccggc ggcgccgttg
     1921 cgccgccggt gcacaccata tcgactgcat caatcgccat ccggtgccgg cggcgatcca
     1981 ccctttcatg acccggcgct taatcgggga aaaatgtttc tcgatataac gaatgcgact
     2041 gaactcccac acgagctgca gacgttcgtg atcgcggccg ctgctgcggc cgatgaagtg
     2101 aataatttcc gcctcggcga gatgacgcac acgatacccc tgctgacgca gacgccggca
     2161 cagatcgacg tcttcaaagt acataaagaa atcttcgtcc aatcccccca attccaatag
     2221 gagcgatcgt cggaccatca aacaggcccc gttcgcccaa tcgacatctt gggattgcgt
     2281 acaatcgacc gcgtgcatcg ccgcggcggc cgcttgcatg gtcggcgcga ctcgttggaa
     2341 cagggctgaa tagaagaggt gatcgcgaat ggagggaaaa gggaaacagg tctgattcag
     2401 gtatccatct cccgtccatt gtttacaacc caccgccccg atgtcaggat gatcgtccaa
     2461 gtgcgcgacc atggtcgcaa gcgcctgatc tctgacaatc gtatcgctgt tgagcaacag
     2521 cacatgccgg ccacggctga cagccaaggc ctgattcacg gccttcgcga atccccgatt
     2581 gtccggattc gcaatgatca tcatgtcagg atagattttc ttgatcgcgg gcaccgtccc
     2641 gtcccgcgaa taattgtcga caacgatcac ttcgtaggcg atggccgtcg tgtgagcccg
     2701 cacggaacgc acacaatcca ataggatgtc cttcgtgttg taactcacaa gcgcgatcga
     2761 cagatcgatc gccggcagag tcgaatcttg tgtatcagtc ggtgtcatac ccactcattg
     2821 ttacgcatcg aactctgcca cgatgatctg ccggtagcag ggaccggcat tgagccagcg
     2881 ccgcacatgt accaaggcaa cggccagacg atgcaacacg taactgaccc ggtccgtccg
     2941 catccacagg cgtaggagta tccgatgcac ccacacattc agattcggtt cgcagtgcag
     3001 tcgcatgggt ctcccgctct ccttggcaat accttctatc tcggccaaga ccgcctccaa
     3061 tctgggaagg ccgttggcaa tatgctcatc gagaaatgga aaggatttgc cgttctgtgc
     3121 ccgataatat tgttgcaagg catgatcttc ccgttcggac tgacgtccca ttggacagcc
     3181 gatgtaaacc cggcgcgtag tgacccgtat cagctcccgc aggaaatggt tccggtcgga
     3241 acactcaata tgttccagcg tatccataca gacggtgaag tcgaacgttc cggactgaaa
     3301 cggtaacgcc tgccccttcg caaggacagc ggtcacaagt ggcacgcgtt cggcactcat
     3361 gacacaatct accccgatga ctggccgacg aagataggtt gtcaagcccg aacggttcac
     3421 ccctacctcc agaattaaac ctcgggacgg ctcggcacaa atcgattcga ctaccggcaa
     3481 gtaacgcaat gcaatttcag gcacccattc gggatagcgt tgattccacc aatcctgtac
     3541 accctgcatc atcgtcttcg tcatgtgtca gcgatgctcc cagagaactt tcaaccgtgg
     3601 cacctccatc gaccagagat accagccata gagtccaagg gctcccaggc aggtggagac
     3661 cagcacccag cctgtaggta agttcgcggt tgtctcggac atgaggagaa taatcccggc
     3721 aaggaagccc gcgaggagag cagaccgtcc agcagaccag aatgtggcat tcaccgccgt
     3781 tcccatgagg gacctggtcc ccatactttg caacagcact ccgaccgcgt agctggcaac
     3841 cagcgcggca gcggcgccgg taaggccaaa tcccatggtc aacggcacaa tcaccatggc
     3901 atagaagaga aactgtgccc cccaactctt catctggaga tcgggccggt tcgccccata
     3961 gtgcaaggcg gtcattacgc ttgaataccc cgcaaagacg acatacacgc agagcattct
     4021 cagtgggtcg acaatggcga gccactgagg accaaaggcc aaaggcacaa tgaccggggc
     4081 gagcacggcc acgctggccg ccagtgggat gaagatcagt ccaaccaggc ccatttgaag
     4141 ttcgaataca cgggccacac cgggtcgatc ctgttgaagt aacgcgtagg taggcatgct
     4201 gatctgatgg atcaacaccg taacccgcac acccaacaag agcggaatgg ccaacgcgat
     4261 ctgatagaaa cccaacgcct cttgcccgag cacgcgaccg atgaccagct ctcccccact
     4321 catgacaccg aagatacaga gcgtcgcgac atttaattgt tttccatagc gcagaaagta
     4381 atcgcatgca ggccggtaga gcgacaggcg tggccggaac ggtgccaccc aatatgataa
     4441 gagactacct atacacatcc cgaccatttg acccagtagc agcgcccata cactgcggag
     4501 ccacaacgcg agaatgatga ttgcggtcgc ctcaatcacg cgccggatga gatccatggt
     4561 gacacgccgc cgcaaattta agttctttag gacaatggcc atcgccggac tctgcagccc
     4621 ctgcagcacg agcaaccagg cgtgtacccg caataatgac gccaactgag gcatctgcat
     4681 ggcctgacct atcacctcag ccgcagccca gagacaggcg gccatggcga ctccccgcac
     4741 gatcgcgacc gtccaggcga cagctaaatc ctcccggtca accttcgcct tggcaatcaa
     4801 agccgattcc agaccgagct cactgatggt catgaatgcc aggacaatgg tggcggatgc
     4861 ggccatcacg ccgaaatcgg ccggaactaa caggcgtgcc accaccatga gctgaaccgc
     4921 cgcaatcact ttatccaaca caaaacctac cccagcccat ccaccagcct ggagaatccg
     4981 gtggcgcaga cgaggcggtg ctggttcagc ccggtccatt ttgacctggg tttcttccag
     5041 caccatgtca cgactggtga tcatgttgtc cgttgcctcg cggcatgcgc tgcgctgcac
     5101 acctgttcca gttctttggc tcgggcatcc caggtatggg cctggatgac cgccatacgt
     5161 cctcgttcgc ccattttcat ccgctcaaca ggatcttcaa gaagacggac caattgccgg
     5221 gccacatccg atgcatcgcc tggttttgct gcaagcccac agccgatggc ctccactgtt
     5281 tccccatatc ccggcccgat ggtcgtgaca acggggcgac cacacgacag atattcccac
     5341 aatttcatgg aatcaccagg ataggatcga gtttgccgat ttaacaccac acacacatcg
     5401 caggccgcaa tccatgtcgg gacccgggcc caatcggccc gaccgacaaa gcggaccctc
     5461 tctgccaatc cttgttgctg aaccaactgt tcgagcgcga atttcgtttg gccttctcct
     5521 accagcaaaa atgtggcgga ggggaccttg gcatgcacga aaggcaccgc atcgattaac
     5581 gtttccaacc catgccaggg aaagaatccg ccgacaaatc cgatgtaggg accatcgaca
     5641 gaaagtccga gagctgttct cgccgcgtcg tgcggttgag gatgaaagcg atcctcgtcg
     5701 actccattgc gaatcacatg acaactctcc ggtggaatac cccaggcatt ttccatctgc
     5761 gccacaatct cgcggcagat gctcaccacc gcctgcaccc ggcacgcagc cttcttcaac
     5821 gccgcgcgaa gtatccatcg tacaaatggg agccgccaga ccccctgcac ctcttcctcc
     5881 ggcagaccat tgacaaaaag caccgtcgca caagcatgat cggacagggc cagcagtggg
     5941 gcgatctgcc ctggcgaatc gaaccacagc aggacctccg gccgccacgc cgccagttcc
     6001 tgcctcaaga catgccagga agctatcaga aatgagaagg gccgaagacc agc
//
LOCUS       DATIPF010000284         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_938638, whole genome shotgun sequence.
ACCESSION   DATIPF010000284 DATIPF010000000
VERSION     DATIPF010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938638"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VLL06_07115"
     CDS             complement(<1..904)
                     /locus_tag="VLL06_07115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSS30770.1"
                     /translation="MGTTCGLSRNGPFREQLHAIAVPTHALSLPPWRKTFALFQRGSA
                     VRCLGALVEQLDPAMIHVNDIWWVPHTVKAVARHKSNSVPIVAHVRQEIEPAKVRRYG
                     LDRVDAVIAISRQVEQSLIAGGVSAGHVRTVYSGIELSGRSLAQDSLAIRSMIGVTNE
                     SVLLGTIANLFPRKGYEVMLRALPAIVRAVPTVHYVIVGSDDHDYADRLKGLAQELKI
                     ADRVHIVGFQDPVQPFLASLDLYVHPALMEGFGIAVVEAMAMGKAVVATTTGGLPEVV
                     AQGETGLLVPPGDAESLAQAVISLL"
     gene            complement(823..999)
                     /locus_tag="VLL06_07120"
     CDS             complement(823..999)
                     /locus_tag="VLL06_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30771.1"
                     /translation="MATKILYVHGIEAIGGAERDLIALLKGLDRQKWEPHVVCPGMAR
                     SENSSTRLQFRLMR"
     gene            complement(999..1793)
                     /locus_tag="VLL06_07125"
     CDS             complement(999..1793)
                     /locus_tag="VLL06_07125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSS30772.1"
                     /translation="MSVASGLSTVACVVITKNEEANIQDCLKSAQWTNELLVVDAESR
                     DRTVELARACGAKVSVRPWAGFGLQKNFGMAQASSDWILILDADERVTEELRREITDR
                     LKGWSSGAPVAYRIPRRNFFYGAWVQRGGVYPDYQVRLFRRGMARYNDVAVHENLLVD
                     GEIGTLAGHIDHFTERRIQDHFKKFGLYTTLAAQEKAKTVQTVRWTDLVFRPLVVWVK
                     TYGLKQGYRDGVRGLIVCVFASMYTFVKYAKLWDTTRQAASQLDRR"
     gene            complement(1790..>2447)
                     /locus_tag="VLL06_07130"
     CDS             complement(1790..>2447)
                     /locus_tag="VLL06_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30773.1"
                     /translation="LTDWMQARWAAIGQGRRSSGKGIEQLRAMTQAMYGDWERYCEEA
                     HRAFAGTMFDGIVGSCVMIRRSTYDVLGGLDERIQSADWDLYYTLRQREQLMGDVKRC
                     MVVGASFVHHFIRATIKSKREPFACTHPRLSIPEKWSIEQQEALWCKPNYFRSASDER
                     EFQQILRRRVGKPLRKLAQEFNRAFSWRWRYVSPEKIVALHRRQFQQAATMLPSGVSS
                     "
BASE COUNT          543 a          775 c          634 g          495 t
ORIGIN      
        1 ccaggagtga gatcacggct tgagccaatg attcggcatc tcccggcgga acaagcaagc
       61 cggtctctcc ctgcgccacg acttcgggaa gccctccagt cgtcgtcgca accacggcct
      121 ttcccatcgc catcgcttcg acgactgcga tcccaaagcc ctccatgagt gccggatgca
      181 catacagatc aagggaagcc agaaacggct gaaccgggtc ctgaaagccg acaatatgca
      241 cgcgatcggc aatcttcaac tcctgcgcaa ggcccttcaa tcgatcggca tagtcgtgat
      301 cgtcactgcc gacaatcacg taatgcaccg tcggtaccgc acggacgatg gccggaaggg
      361 cccgaagcat gacctcgtag cctttgcgtg gaaacaggtt cgcgatggtg cccagcaaga
      421 cggattcgtt cgtcaccccg atcatcgagc gaatcgccag gctatcctgt gcaagtgatc
      481 tccctgaaag ctcgatgccg ctgtagacag tccggacatg gcccgctgag accccaccgg
      541 caatcagcga ctgttcgacc tgccgagaga ttgcaatgac ggcatcgaca cgatcaagcc
      601 catagcgccg caccttcgcc ggctcgattt cctgtctcac atgggccacg atcggcacgg
      661 aattagactt atggcgcgcg accgccttca cggtgtgggg cacccaccag atatcgttca
      721 catggatcat cgccggatcg agttgttcca cgagagcgcc cagacaccga actgccgatc
      781 cgcgctgaaa cagtgcgaag gttttcctcc aggggggaag gctcaacgca tgagtcggaa
      841 ctgcaatcgc gtggagctgt tctcggaacg ggccattccg ggacagacca catgtggttc
      901 ccacttctgg cgatcgagtc ccttcaacag tgcgatcaga tcgcgttcgg ccccaccgat
      961 ggcttcaatc ccgtgcacat acagaatctt cgtagccact acctgcggtc cagttgagag
     1021 gcggcctgcc tggttgtatc ccacagcttg gcatatttca caaaggtata catgctggca
     1081 aacacacaga caatgaggcc acgcactcca tcgcgatagc cctgtttcaa tccataggtt
     1141 ttgacccaga ccaccagcgg gcggaacacc aggtcggtcc agcgaaccgt ctgcaccgtc
     1201 ttggccttct cttgtgccgc cagtgtggta tagagcccga acttcttgaa gtgatcctga
     1261 atgcgccgct ccgtgaaatg atcaatgtgt ccggccaatg tcccaatttc tccatcgacg
     1321 aggagatttt catgtacggc cacgtcgtta tatcgagcca tccctcgccg aaacagccgc
     1381 acctgataat caggatagac tccgcctcgt tgcacccaag ccccgtaaaa aaagttccgc
     1441 ctgggaatgc gataggccac aggagcgccg gacgaccacc ccttcagacg gtccgtgatt
     1501 tcccggcgta gttcctcagt gacacgttcg tccgcatcga ggatcaaaat ccagtcagac
     1561 gacgcctgtg ccatcccgaa gttcttctgc agaccgaagc cggcccaggg acgcacagac
     1621 acctttgccc cgcaggcacg ggccaactcc acagttcgat ctcggctctc ggcatcgacc
     1681 acgagtaact cattggtcca ctgagccgac ttcagacagt cttggatatt ggcttcttcg
     1741 tttttggtga tcaccacgca cgccacggtg gaaaggccgg aggcaacact catgacgaca
     1801 ctccactggg cagcattgtc gccgcctgct gaaactgccg gcgatgcaac gccacaatct
     1861 tttcggggct gacatagcgc cagcgccacg agaatgcacg gttgaattct tgagcaagct
     1921 tcctgagagg cttcccaacc cgtctgcgaa gaatctgctg gaattcacgc tcgtcggacg
     1981 cggaccgaaa ataattgggc ttgcaccaga gtgcttcttg ttgctctatt gaccatttct
     2041 caggaatgga gagtcgcggg tgggtacagg caaacggctc ccgtttactc ttaatcgtcg
     2101 cccggatgaa atgatggacg aatgacgctc cgacgaccat gcatcgcttc acatccccca
     2161 tgagttgttc gcgctggcgc aaggtatagt ataagtccca gtcagcggac tgaatccgtt
     2221 cgtccaaacc gccgagtaca tcgtaggtcg atcgtctgat catgacgcag gacccgacga
     2281 tcccgtcgaa catcgtgccg gcaaatgcac ggtgcgcctc ctcgcaatag cgttcccaat
     2341 cgccatacat cgcctgagtc atcgctcgta gttgctcgat gccctttcca ctggaacgcc
     2401 gtccttgtcc aatcgccgcc catcgcgcct gcatccagtc ggtcaaa
//
LOCUS       DATIPF010000285         4050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_409258, whole genome shotgun sequence.
ACCESSION   DATIPF010000285 DATIPF010000000
VERSION     DATIPF010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409258"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VLL06_07135"
     CDS             complement(<1..927)
                     /locus_tag="VLL06_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSS30774.1"
                     /translation="MQEPRPFLIGGLWRHGDTVVPVNNPFTGRLLAEVSNASSADAED
                     AIQSTVDAAVAMGVLPSHARYHALQKIAGGLYDRREEFARLMTAEAGKPIADAKREVN
                     RAVQTFTVAAEEARRIPGEVIPLDWTPGTDSHLGILRRVPIGPVLGITPFNFPLNLVA
                     HKVAPALAAGNSILIKPAPQAPLTALLLGEVALGAGLPPGALNILPCNNTIAEQLVAD
                     PRFKLLSFTGSAAVGWMLKAKSGKKKVVLELGGNAGVIVEPDADLEVAAQRCAVGGFG
                     YAGQTCISVQRIFVHHSIVDLFTTKLLLQVARM"
     gene            complement(955..1224)
                     /locus_tag="VLL06_07140"
     CDS             complement(955..1224)
                     /locus_tag="VLL06_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30775.1"
                     /translation="MSATQSFWSVPQRAGEPAYWVCMSCLSEVFYCKVPMPDCPTCQG
                     VSTYEAFTLEAIRDWGTEDLIAKAGIAQQEASSEAVPAASVESAE"
     gene            complement(1234..1941)
                     /locus_tag="VLL06_07145"
     CDS             complement(1234..1941)
                     /locus_tag="VLL06_07145"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSS30776.1"
                     /translation="MMGTRWFYAVVGALLLALVQLAETEGGAVADDGRIRGRADAPIT
                     LIEYSDFTCGYCGKFFRETWPRLQAKYIETGKVRFVYRDYPRSDQGTGVEAAVAARCA
                     GAQGRYWQMHDRLFSERGRLDSGSFKSYAKVIGLDETSFAKCFDEREYLESIFRDRQE
                     ANRWGFHGTPGFILIRTVGGPTEKEPAVAIPGAVPFEDFAEEIDRMLATAPRTQGELT
                     EPQGSTAVAQSSQSIIY"
     gene            complement(2688..3335)
                     /locus_tag="VLL06_07150"
     CDS             complement(2688..3335)
                     /locus_tag="VLL06_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSS30777.1"
                     /translation="MNRHNREAKPSATRGARVKDALIVDDHPIVRAAIKDLLEKSFPD
                     IRVTEFDGSTSVVNKICGGNWAFVVLDINLPKKNGLDILKAVKVAGNHTPIVVFSLYA
                     EEHYAARALRAGAKAYISKNRASLDLVNTINTLLKGGRVKPSPSVIAPRPVLSDREIQ
                     VLSLLTKGMSRKEVAQTLNIHEKTVSSYRARILVKLNARTLVDLIHYATEEGFVD"
BASE COUNT          946 a         1173 c         1047 g          884 t
ORIGIN      
        1 catccgcgca acttgtaaca ggagcttcgt ggtaaagagg tccacgatgg aatgatggac
       61 aaagattcgc tgaacggaga tacaggtctg ccctgcatac ccgaatccgc cgactgcgca
      121 acgctgcgcg gcaacttcaa gatcggcatc gggttccacg atcacgcccg cattcccgcc
      181 aagttccaat accaccttct tcttcccaga cttggccttc aacatccatc ccaccgcagc
      241 actgccggta aagctcagga gtttgaagcg aggatcggcc acaagctgtt ctgctatcgt
      301 attgttgcag ggcagaatgt tcagtgcgcc agggggaagt ccggccccta gagccacctc
      361 acccaagagc aatgcggtca gaggcgcttg tggcgccggc ttgatcagaa tggaattgcc
      421 ggcggccaga gccggagcaa ccttgtgagc cacaagattc agaggaaagt taaagggggt
      481 gatcccaagc accggaccaa ttggaacccg gcgaagaata ccgagatgag aatccgtccc
      541 tggcgtccag tcgagaggga tcacttcccc gggaatccgc ctcgcctctt cagccgcaac
      601 cgtaaatgtc tggacagccc gattcacctc acgcttcgcg tcggcaatcg gtttgccggc
      661 ttcagccgtc atcaggcggg caaattcctc acgtcggtca tagagcccgc ccgcgatctt
      721 ttgcaacgca tgatagcggg catgggacgg caaaaccccc attgcgacag cggcatcgac
      781 cgttgattgg atcgcatcct cggcatcggc ggagctcgca ttcgacacct cggcaagcag
      841 gcgccctgta aaaggattgt tcacgggaac aacggtatcc ccgtgccgcc acagacctcc
      901 aatcaaaaat ggacgtggtt cctgcacgag agatgcctcg cgagtggaga aggcttattc
      961 ggctgactca accgatgcgg caggcaccgc ctcggagcta gcctcctgct gggcgatacc
     1021 ggccttcgca atcaagtcct ccgtacccca atcacgaatc gcctcaagcg tgaaggcctc
     1081 gtaggtcgag acaccttggc aagtcggaca atccggcatc ggcaccttgc aatagaagac
     1141 ttctgacaag catgacatgc agacccagta agcaggctcc cccgcacgct gcggaacaga
     1201 ccagaacgat tgtgttgcag acataggtaa tccttagtaa atgatggact gactgctctg
     1261 tgctaccgcc gtcgacccct gaggctctgt gagttctcct tgggtgcgcg gggccgtggc
     1321 caacatgcga tcgatctctt cagcaaaatc ttcgaacggg actgcgccgg gaattgcgac
     1381 cgccggctct ttctcagtgg gcccaccaac cgttcggatc agaataaaac cgggagtgcc
     1441 gtgaaatccc caccggttcg cctcttggcg atcccgaaaa atggattcga gatattcccg
     1501 ctcgtcgaaa catttggcaa acgacgtctc gtctaacccg atcacctttg cgtaactttt
     1561 gaacgatccc gaatctaagc gacctcgttc gctgaacaac cgatcatgca tctgccaata
     1621 tcgaccctgg gctccagcac agcgtgctgc gaccgccgct tcaacaccgg ttccctggtc
     1681 cgatcgagga taatcccgat aaacaaaccg caccttgcct gtctcgatat atttggcctg
     1741 caagcgaggc caggtttccc tgaagaattt tccacaatag ccacaggtaa agtcagaata
     1801 ctcgattaag gtgattggtg cgtcggcgcg tcccctgatc cgtccatcat ccgccaccgc
     1861 ccctccttct gtctcggcta actgaacgag ggccagcagc aatgcaccga caactgcgta
     1921 gaaccaccgt gtgcccatca ttcgaaagac ctaatctgct ccggatcgag aggctcaatc
     1981 gcttccaggc catctttcgc tccatcgtga aattcacgcg tacccataaa agcgcgtcta
     2041 cctgcattga agtgaatgga gaaacggcgc attctttata cacaaccaac gatagaggcg
     2101 tcaacgaagt tgggccacga taccctgacg ctgcatcctg cgcggcagta cacatgacct
     2161 gttcatagaa ccacaaccgt cggcacaatc ttgaaagcac gagacgtggg ttcagcgatc
     2221 tctacgacag tactcaggaa agcagcctgc ctcactggca gtgatcatac acggaacgca
     2281 caggagaggt tatcccagcg caatgtactc tattaatagc tctgagccct gcgagcttgg
     2341 tgtggtggtc ccaacgggat ttgaacccgt gtttaagcct tgagagggat agtcagaggc
     2401 aaatatcgca aacactcaat tgggcggtct tcccacttta ttccgtattt tccagttgtt
     2461 atgaacggaa aaatcccgat ccccgattct atgaattctc accaatgctg taaaatctat
     2521 gcgttctgtc acgctggtgt cacggtccat cgcagaaatt tgtaacccgg tccagacttc
     2581 caagagggac tgatgccgac gtgtaatgcc tcaaaaccca gtcttgccaa ggcgcgcact
     2641 tctcatggaa caaagtgtgc caactttcaa ccgtcgttca ctcaccctca gtcgacgaac
     2701 ccttcttccg ttgcgtagtg aatgagatct accagcgtgc gcgcattcag tttcacaagt
     2761 atccgtgcgc gatagctgct gacagttttt tcatgaatgt tgagcgtctg ggctacttct
     2821 ttccgggaca tccccttagt caagagcgaa aggacctgga tttcgcggtc cgacaggacg
     2881 ggccgaggcg cgatcacgga gggagagggc ttcacccggc cgcctttcag gagcgtgttg
     2941 atggtgttca ccaggtcgag cgatgcgcgg tttttcgaga tatacgcctt ggcacccgca
     3001 cgtaatgcac gggctgcgta gtgctcctcc gcataaaggc tgaatacaac tattggggta
     3061 tggtttccgg cgaccttgac cgctttcaat atgtcgagac catttttctt gggcagatta
     3121 atatcaagga ccacaaacgc ccaattgcct ccacaaatct tattcactac tgaagtgctt
     3181 ccgtcaaatt cagtgacccg aatgtcaggg aacgactttt ccagtaagtc tttgatggct
     3241 gcccggacaa tgggatggtc gtctacgatt aacgcgtctt tcaccctggc ccccctggta
     3301 gcgctgggtt tggcttcacg attgtgtctg ttcattgagc tcggtgcatg cgacctagac
     3361 cggctggatc atgaggaggt gactcttaat aacaatctga tcgaccatgc ttattgtgct
     3421 ccgtgcttcg ggagtgctcc tcgagctctg cgagtggctc gagcatgaga tatgtattgt
     3481 gtcgggcata ccatgcctcc gaccagtcca gcttcgcgag caggcccgaa acgcggcaat
     3541 aaggaaatgg tttatacaga agaagagaga tcgtgtctgt aagcagccgc ctacatttct
     3601 gccgatccac gtgactatca ggaccgctga gcttcctcgc catacccgag accttcgaaa
     3661 ataacgaagc gcattccttg agccaagaga agatcctgga ggaggggtgt ggaggaacgg
     3721 atgggccacc agaaataact tttcagtgat ccctggaagt ccggtctgag cgcaagtcat
     3781 tgtcggcaaa atgcggtctc gtacggcttt ctacttcccc tcactccgcg tcagaggtcc
     3841 tctccgcctc gaagccttga ggggaaatgc accaaacatt cctatggggt tatctctata
     3901 acggtctgaa tggcttccct gatcgatcga atgggcaaaa cctgcatggc tgaggacccc
     3961 gctccagggc gctccaccgc cctttgctga gtggaaatga gaacccgtcg gatattggct
     4021 gcatcggctg ccgcaagctt cagagacacg
//
LOCUS       DATIPF010000286         6945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_321707, whole genome shotgun sequence.
ACCESSION   DATIPF010000286 DATIPF010000000
VERSION     DATIPF010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321707"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VLL06_07155"
     CDS             complement(<1..195)
                     /locus_tag="VLL06_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30778.1"
                     /translation="MQVGAAMELLRPLLADITADGLSLVGEATAEELGLTEDDAVVVG
                     PLAVSLELTNVEGLVAVTGVL"
     gene            complement(217..471)
                     /gene="rpsR"
                     /locus_tag="VLL06_07160"
     CDS             complement(217..471)
                     /gene="rpsR"
                     /locus_tag="VLL06_07160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HSS30779.1"
                     /translation="MEREQGGDREGGGRFFQRRRPCRFCIDQGSIDFKDVGLLRNFLT
                     ERGRIVPRRISGNCMRHQRELTAAVKRARHIALISFAEER"
     gene            complement(490..891)
                     /locus_tag="VLL06_07165"
     CDS             complement(490..891)
                     /locus_tag="VLL06_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HSS30780.1"
                     /translation="MAGFNKVVLLGNLTRNPELRYTPNGTPVASLGLAVSRRYKQGEE
                     LKEEVCFVDIVVFGKQAEHCGQYLSKGNGIIVDGRLQQRRWETEDGQKRSKHEVVAQT
                     VTFMPKRQDSGGGGIEAPVHDDAGYEPEEHV"
     gene            complement(878..1225)
                     /gene="rpsF"
                     /locus_tag="VLL06_07170"
     CDS             complement(878..1225)
                     /gene="rpsF"
                     /locus_tag="VLL06_07170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HSS30781.1"
                     /translation="MELYESLFIIRSSVSDQETAALIEKMKAVAEKTGAQFIKSENWG
                     KKKLAYEVKRERKGTYVYFYFRAPNVTVGELERSYRLEDSIIKFLTVHLKKELVPPRP
                     IETSSEEFAGGRI"
     gene            complement(1973..2869)
                     /locus_tag="VLL06_07175"
     CDS             complement(1973..2869)
                     /locus_tag="VLL06_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase response regulator"
                     /protein_id="HSS30782.1"
                     /translation="MIRVLLVEENEAEARDACSMLQQAGEGQMEVIRVAKLSAALRRL
                     SSETFDVILLDGALVDTHGLTTLDLVQAALASMPLVLLSNQDDPALERKAIQQGVQDV
                     IVKKNWTREHLIRSVHHAVDRKRAEKSLAYLAQYDPLTALANRALFRDRLTHALALAK
                     RKKQEVGVVLLGLDRFKNMIEQLGSEIGDALLSVTADRLKQCMRDVDTVARLSGNEFT
                     CLLEGITCKNDMEIVARRILASMDKPVQMQDQTVGISASLGLAVFPLDGQEADGLLDC
                     AKQAMVCAQEAGGAGYQLYTVS"
     gene            complement(3044..4135)
                     /gene="ychF"
                     /locus_tag="VLL06_07180"
     CDS             complement(3044..4135)
                     /gene="ychF"
                     /locus_tag="VLL06_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HSS30783.1"
                     /translation="MGLCCGMIGLPNVGKTTVFNALTGSGALAANYPFATVDPNTGIA
                     LVPDPRLSVLTDLFKSKKTTYSTLEVRDIAGLVEGASKGEGLGNQFLGHIREVDALLH
                     IVRCFQGTDVVHVSGGVNPLRDIGVIETELLLADLDTLDRRKQKTEKKVRAGDKKAAF
                     ELTFIDKLIGLLDKGEWLGNLTYTPEERMVLDECQLLAAKPVLFVANVSEQAGADEAM
                     VKSVREFAEKRGARVVTICGQLEAELSTLPESERVDFLKEMGLMESGLVRLTREAYTL
                     LDIITFFTAGEIESRAWPIPKGMKAPQAAGKIHSDMERGFIRAELYHYNDLLACGSEA
                     KVKEKGLFRLEGKDYVIKEADIVYFRFNV"
     gene            complement(4289..4888)
                     /gene="pth"
                     /locus_tag="VLL06_07185"
     CDS             complement(4289..4888)
                     /gene="pth"
                     /locus_tag="VLL06_07185"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HSS30784.1"
                     /translation="MHLIVGLGNPGTAYAQTRHNVGMWVIEQAAARWSIRLAKRGVAH
                     RGTGRLGSEFLELAGTLDWMNITGLPLKGLLRECSLTADDLILVHDDLDLDLGRLRIK
                     QAGGHGGHNGIKSVIEAIGTPQFVRIKIGIGRPAPRQDSADYVLQPFTREEIEILNPC
                     LDHAVDALECLIHRGTAVAMNQFNVREKVGGEEEEGEPG"
     gene            complement(4891..5676)
                     /locus_tag="VLL06_07190"
     CDS             complement(4891..5676)
                     /locus_tag="VLL06_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HSS30785.1"
                     /translation="MKFELAVSVREKTGKGAARQLRREGKVPGVLYGQGECLLLTIEP
                     DSLIKILKAQAGGAALVSLTLSGAKSKPNRTALLRDFQVDPVEGNVLHADLFEISMNK
                     PIRVKVPLHLTGGMPAGVKEGGILHHNMRELHIECLPAVLPDFIEVDASGLAIAQGLH
                     LKDLAVREGIRYLDDPEQMVVSVAAPMTEAKLESLLASGAAAPDAAKEPEVATKGKVA
                     GEVPEGGEAAKPGTAAVAADAKGGEKKGAPAPKAEKKEAEKKK"
     gene            complement(5727..6656)
                     /locus_tag="VLL06_07195"
     CDS             complement(5727..6656)
                     /locus_tag="VLL06_07195"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HSS30786.1"
                     /translation="MKIFSGNANPALAHEICAYLGTKLGEATVGSFSDGEIRVKIEEN
                     VRGGDIFVVQSSCQPVNDSLMELLIIIDALKRSSANRITAVIPYFGYARQDRKDQPRV
                     PISAKLVADLISTAGTDRVLTMDLHAGQIQGFFNVPVDHLYALPVLLDYITKKKVSDL
                     VVVSPDAGGVERARAFAKRLEASLAIIDKRREGPNQTAIMNIIGDVAGKSALLLDDMI
                     DTAGTIVKGAQACMDRGAREVWTACTHAVLTGPALDRIQQSCLKQVIVTNSIPLRGKE
                     LICPKLYQLSVAPLLGEAIRRIHEDESVSSLFA"
BASE COUNT         1489 a         2156 c         1730 g         1570 t
ORIGIN      
        1 aagcacaccg gtcacggcga ccaacccttc gacgttggtc agttccagac tgacagccag
       61 cggaccgacg acgacggcat catcctcggt caggccgagt tcttcagccg tcgcctcgcc
      121 gaccagcgaa agcccatcgg cagtgatgtc agccaacagc ggcctgagca attccatcgc
      181 ggcacctacc tgcatcccta tccccaacgt gaccggttat cgctcttccg caaagctgat
      241 gagagcaatg tgacgagcac gtttgacggc agccgtcaat tcgcgctggt gcctcataca
      301 attgccggag atgcggcgcg gaacaatccg accccgctcg gttaaaaagt tcctcagtag
      361 cccgacatct ttaaaatcga tcgatccttg atcaatacaa aacctgcacg gccgtctgcg
      421 ctgaaaaaac cggccgccgc cctctcgatc tccgccttgt tcacgctcca tcaccgtctc
      481 cctctctgtt tagacatgct cttcaggttc gtacccggca tcatcatgca ccggcgcctc
      541 aattccgcca ccgccgctat cctggcgttt cggcatgaag gtcaccgttt gggcgaccac
      601 ctcgtgcttg ctgcgctttt ggccgtcctc tgtttcccac cggcgctgct gtagccgacc
      661 atcgacgata atcccatttc ccttactcag gtattgtccg caatgttccg cctgcttgcc
      721 gaagacgaca atgtcgacaa aacagacttc ttcttttaat tcctcaccct gcttgtatcg
      781 ccggctgact gcaagaccca aactggccac cggcgttccg ttcggcgtgt atcgcagttc
      841 aggattccgc gtgaggttcc ccaggagaac gactttatta aatccggcca ccggcaaact
      901 cctctgatga cgtctcgatc ggccgtggcg gcaccaactc ttttttgaga tggacggtca
      961 agaacttgat gatggagtct tccagccgat aggatcgctc gagttcacca accgtgacgt
     1021 taggcgccct gaagtagaag tacacgtacg tgcccttccg ctcacgcttc acctcgtagg
     1081 cgagcttctt cttaccccaa ttttctgact tgatgaactg cgcgccggtc ttctctgcca
     1141 ccgccttcat cttttcgatc aaggcagcag tctcttggtc ggagacagac gaacgaataa
     1201 tgaacaggga ctcgtagagc tccatgcagc aatcctcctg gacaaaggcc cccaatcttg
     1261 gagcctatcg gataatcccc tgcaactacg gcgaatgatt caaggccatc tgcttcgttc
     1321 tcggcctgaa aaaatcctca acgtattaca gagaatacgc ttccggtgtt tttcaggcct
     1381 gcggccttgc agctagcctt ccctcattcg cctcattacg aaggagatat ccgacaggct
     1441 ccattgggag cgaggtgtag gcgcctctat atgaggctga aagaacggtg gacggtatca
     1501 cagccacaga gaaactgtca atttattgag gcttcacact ggacgaaaca gagcctgccc
     1561 aatctcatct gattcccggc ttccgagtac agtcccacgg gttgccccga gcggagtcaa
     1621 tctggaattc cctttgccgt tatctcatga tcgaggactc tgcatatcac tccgtcgagg
     1681 ctgctcaaaa atgcggataa caaggccgca gggagcgcag agactgacgc gtacctttta
     1741 gagcgcacgg tgcgacgaat aggagcacca cgtctatagc gcgccgccga gtcggtgaag
     1801 cggccgggtc cccttcgagg atttcgacga gccgagaacg gcgcatgagg agagatgcgt
     1861 cctggggtta agaggctgtc ttggcagact cagggctggg tcggtgaaat aatcgcctgg
     1921 gtcgggcggg tgcgaagagt gctttttcaa cagcctgata gcggggttcc cgtcacgaaa
     1981 cggtatagag ttggtaccca gctccaccgg cttcttgggc acagaccatg gcctgcttgg
     2041 cgcaatccag taacccatcg gcctcttggc cgtcgagtgg aaacactgcg aggcccaagc
     2101 tggctgagat acccacggtc tgatcctgca tctgaaccgg cttgtccatc gaggcaagaa
     2161 ttctcctcgc cacaatctcc atgtcattct tacaggtgat tccctccaac aagcaggtaa
     2221 attcattccc gctgagcctc gccaccgtat cgacatccct catgcactgc ttgagacgat
     2281 ccgctgtcac ggaaagcagg gcatcgccga tctcagaccc caattgttct atcatgtttt
     2341 tgaagcgatc caatcccaac agcacaaccc ccacctcttg tttcttgcgt ttggccaacg
     2401 ccagcgcatg cgtgaggcga tcacggaaca aggcccgatt ggccaaggca gtcaatggat
     2461 catactgcgc gaggtaggcc aagcttttct cggcccgctt gcgatccacg gcatgatgca
     2521 ctgaacggat gaggtgttcc cgtgtccagt tctttttcac aatcacatcc tgcacgccct
     2581 gctgaatggc ttttcgttct aacgctggat cgtcttgatt gctcaacagc acgagcggca
     2641 tactagccaa cgcggcttgg accaaatcca gagtggtcag gccatgcgta tcgacaagtg
     2701 caccatccag caaaatcaca tcgaatgttt cgctgctcaa acgccggagc gccgcgctaa
     2761 gtttcgccac acgaatgact tccatttgcc cttcccccgc ttgctggagc atagagcagg
     2821 catcccgggc ctccgcctca ttctcttcaa ccagtaggac tctgatcatg gaatctcttc
     2881 tccttcaaaa ctgccagcct cctagactgc cctgaaacac tagcattcta gtaattcgtg
     2941 ttgagagaag ataccagttg aggcaccaac agaaagcaca agacccatcg agcccgtctt
     3001 gtgttcgaat atggtgcaga ccgttgaata gggaggcgag aggtcacacg ttaaatcgaa
     3061 aatatacgat gtccgcttcc ttgatgacgt aatccttgcc ttcaagccgg aacagcccct
     3121 tttccttcac cttcgcttct gacccacaag ccagcagatc attgtagtga tagagttcgg
     3181 cgcggatgaa tcctcgttcc atgtcggagt ggatcttgcc cgcagcttgg ggggccttca
     3241 tacccttcgg aatcggccag gctcgtgatt cgatttctcc ggccgtgaag aaggtgatga
     3301 tgtccagtaa cgtgtaggct tcacgggtca accgtacaag gcccgattcc atgagcccca
     3361 tctctttaag gaagtccact cgttcactct cggggagcgt ggaaagctcc gcttcaagtt
     3421 ggccacaaat cgtcaccacc cgtgcgccac gtttctcggc gaactcacga accgacttca
     3481 ccatcgcctc gtcggcgcca gcctgttcag acacattcgc cacgaagagc accggcttcg
     3541 cagccagcaa ttgacattca tcgagcacca tccgctcttc gggagtgtac gtcaaattcc
     3601 ccagccattc gcccttatcg agcaatccga tcagtttgtc gataaacgtc aactcaaacg
     3661 cggccttctt atcgccggct cggactttct tttccgtctt ttgctttcgc cgatcaagcg
     3721 tgtcgagatc ggccaagagc aattcggttt cgatgacacc gatgtcacgg agagggttca
     3781 cgcctccgct gacatgcacg acatccgttc cttgaaaaca gcggacaata tggaggagcg
     3841 catcgacttc acggatgtgt ccaagaaact gattgccgag accttcaccc ttgctcgccc
     3901 cttcgaccag ccccgcaata tcgcgaacct ccagcgtgct ataggtcgtt ttctttgact
     3961 tgaagagatc ggtgaggaca ctgagtcgcg gatccggcac cagggcgatg ccggtgttgg
     4021 gatcgacggt tgcgaaggga tagttggctg ccaacgcccc gctccccgtg agcgcattga
     4081 agacggtggt cttccccacg ttcgggagtc cgatcatgcc gcaacagagt cccacttctt
     4141 cgcccccttc aatctagcga gtaagggaag ccaggttgac ctatgatgcg cgcgtccaat
     4201 gagggcctgc cctcgttctc acccgcccac cctcgcggcg ccgagacgcc tcgctccccg
     4261 gtaggccgcg cgttgcacga gcagcagatc aacctggctc cccctcttct tcctcgcctc
     4321 ccactttctc tcgcacatta aactgattca ttgccacggc tgtcccccgg tggatgagac
     4381 attccaatgc atcgacagca tgatcgagac aaggattcag tatctcgatc tcttctcttg
     4441 taaatggctg caacacatag tccgctgaat cctgacgtgg ggccggtcgg ccaatcccta
     4501 tcttaatccg tacaaactgt ggcgtgccga tcgcctcaat gaccgacttg atcccattgt
     4561 ggcccccatg tccaccagct tgtttgatcc ggaggcgccc cagatcaaga tcaagatcgt
     4621 catggacaag aatcaggtcg tcggcggtca gcgaacactc acgcagaagg cctttgagcg
     4681 gaagaccagt gatgttcatc cagtcgaggg tgccggcgag ttcgaggaat tctgacccaa
     4741 gccgacccgt cccacgatga gccacgccgc gcttggcgag acggatggac caccgagccg
     4801 cagcctgctc gatgacccac atgccgacat tgtggcgggt ttgggcatag gcagtacctg
     4861 gattgcccag cccaacgatg aggtgcaagg ttacttcttc ttttcagctt ctttcttctc
     4921 agcttttggc gctggagcgc ctttcttctc gccgcctttt gcatcagcag ccacagcagc
     4981 cgtcccaggt ttcgcggctt caccaccctc aggaacttca cccgccactt tgcccttcgt
     5041 ggccacctcg ggctccttcg cagcatccgg tgccgctgcg ccgctcgcga ggagcgattc
     5101 gagcttggct tccgtcattg gtgccgcgac actgacgacc atctgttcag gatcatcgag
     5161 atagcgaatc ccttcgcgca cagccaagtc cttcagatga agcccttgcg caatggcgag
     5221 cccggaggca tcgacttcga tgaaatccgg caacaccgcc ggtaaacact caatatgcag
     5281 ctctctcatg ttgtggtgaa gaatgccgcc ttctttgacc ccggcaggca tgccgccggt
     5341 caggtgcaat ggcaccttca cgcggattgg cttgttcatc gatatttcga acagatccgc
     5401 atgcagcaca ttcccttcga ccggatcaac ctggaaatcg cgtaggagcg ccgtccggtt
     5461 tggtttggac tttgcaccac taagggtgag cgacacgagt gcggcccccc ccgcctgtgc
     5521 cttcaaaatc ttgatcaggc tatcgggttc aatcgtcaaa agcaggcatt ctccctgccc
     5581 atacaacacg cctggaacct tgccctcccg ccgaagctgg cgcgctgcac cctttcctgt
     5641 tttctctctc actgacactg caagttcaaa cttcatgata cacctccgcc cccttctccc
     5701 tggtattcag atatcacgga ttcaaattag gcaaataatg aacttaccga ctcatcttca
     5761 tggattcgtc ggatcgcctc acctaacagt ggggcaactg ataattggta taacttcggg
     5821 caaatcaact ctttgccccg taatggaatc gaattggtga caatcacctg cttcagacag
     5881 gattgttgga tccgatccaa cgccggtcca gtgagtaccg cgtgggtaca agcggtccac
     5941 acttcgcgag ctccacgatc catacaggcc tgcgccccct tcacaatggt cccagccgta
     6001 tcgatcatgt catccagcaa gagcgcgctc ttcccggcga cgtcaccgat gatgttcata
     6061 atcgcggtct gattcggtcc ctcacgccgc ttgtcgataa tcgccaaact ggcttcgagc
     6121 cgcttcgcga aggcgcgggc ccgctcgact cccccggcgt ctggcgagac caccaccaag
     6181 tcgcttacct tctttttagt tatgtagtcc aacaacacag gcagtgcgta aagatggtcc
     6241 accggcacat tgaagaaccc ctggatctgc cccgcgtgga ggtccatcgt cagcacccgg
     6301 tcggtaccgg ctgtactgat aagatcggcc acgagcttcg ccgaaatcgg cacacgcggc
     6361 tgatctttac gatcctggcg agcatagccg aaatacggga tgaccgccgt gatacgattc
     6421 gccgaggacc gtttgagcgc atcgataatg atcaataact ccatcaggga gtcgttgacc
     6481 ggctggcaac ttgactgcac gacaaatata tcgccgccac gaacattttc ctctatcttg
     6541 acgcgaattt ctccgtcact gaatgatccg accgtggcct cgccgagctt ggtcccaagg
     6601 taggcacaaa tctcatgggc cagcgccgga ttcgcgttgc cagagaatat cttcaactct
     6661 ccgctcatcg attcctcaga ggattcttgg atgcgactgc atcatattct gatttcgtat
     6721 agggttcgtc tcgacggcgg gcgtcggtgg gcgtactcat gacccgccta cctacattta
     6781 cctgcgcgct gttgaggaag ccaactaagg ggtcaaactg taacggaatc cgcggggagt
     6841 tgtcaaccga ggcacggctt accttggttg ttcttctcgc caagccagcg ggcctgaaca
     6901 ggtgggaacc acaaacacct tcatgagatt ctctctcgca aactg
//
LOCUS       DATIPF010000287         6360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_849741, whole genome shotgun sequence.
ACCESSION   DATIPF010000287 DATIPF010000000
VERSION     DATIPF010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849741"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VLL06_07200"
     CDS             <1..854
                     /locus_tag="VLL06_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS30787.1"
                     /translation="TGFSWSNAKPDELWTARAQEPDLPKPIEPVLSTHDQFQSYMKAG
                     QQAEAEEWLSRLVTAQPDDAEARELFGSLLETKGDTAGAALQYSRALELLLAYPQDEN
                     STRPASLYAKVKELAPASPLLAKWASTFSPTPQAPVSAAETPVSAPPVHEINPETHYT
                     LGVAYKNMGLLDEAKEEFCLSLQSQDFFSDSCLMIAVCLKEQGECRSASAQLELLLKD
                     PNCQGAKALAIRYELGLLYEAQEQWSQAAAIYESIPTFHDVPKRLESARAHEATGEPT
                     SAFRYGN"
     gene            complement(1085..2050)
                     /locus_tag="VLL06_07205"
     CDS             complement(1085..2050)
                     /locus_tag="VLL06_07205"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HSS30788.1"
                     /translation="MTDTRPTLYVSRLLPDPVMVIVRERFQLIQGPSDALPSPATLRE
                     GLGQADAAILTLGDRIDAETIHAATRLKILANYAVGYNNIDLATARQCGLIVTNTPDV
                     LTDATADLTWALILATARRVVEGDALVRSGTWTGWSPTQLLSAEVTGKTLGIIGMGRI
                     GQAVAQRSIGFRMPVRYHTRQPQTVSSLPSEWEYRSLQDILGEADIVTIHVPLTQATH
                     HLIGTRELAWMRPTAFVINTARGPIVDEAALVDALNTGIIAGAGLDVYEQEPAIHSTL
                     AQLKQVVLLPHLGSATLHARVQMGMVCLENIQAVLEGRPAPNQIH"
     gene            2256..5081
                     /locus_tag="VLL06_07210"
     CDS             2256..5081
                     /locus_tag="VLL06_07210"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HSS30789.1"
                     /translation="MTAPTLILPERSRAVASDLRAAIGAHKVKDDYSTLTAYAVDASI
                     YRVEPKAVVLVESEEDIAATVRYAVARGIPLTPRAAGTNLTGSAIGSGIILDVSRLNR
                     VLELNREERWARVQPGIVLAELNKGLGRHGLLFGPDPSSGDMCKLGGMLANNSSGPHT
                     LRYGAVKDNVQALRVCLQSGDWLDAKAYGLDDSRCTDLLKAHRPLHDLRSLLQDHAAL
                     IAQKRPTVSKNSCGYNLFGLADGLARGCFDLPKLFVGSEGTLGLFSEATIRLVEKPRA
                     TLTALIHFHRLEDVGDAVPKLLELSPSALEVMDANTLNLIGRAKHGIPADAAATLLVE
                     LDGDAIGSDLRAQAEQMIDISRRYPLTSDPVIAFDPEQREQLWKARKALYPTLYRFDP
                     KKKPINFVDDVVVPAERISELIRYLEDFFAGQHVPVAIFGHIGNGNAHIIPLLDVNDH
                     DDFQKMVEGYHEIHQTVLGRFGGSICGEHGDGRVRAEYVKAMFGPELYDLFVRVKQSF
                     DPTGVLNPGIKISDRPFTDHIDHVRLSKSCATCAKCNAVCPVYDVFQSEDMSSRGWFE
                     IVTDKNYSYLNSKRVVEACLNCKSCRTACPAGVDVSQLILDRRAENPNEMAGVIFRFH
                     AQTARFEQFLKFLARYQSIWDRPLIRRLLDRLTKPFMHRLAETARLSPQLVLPRLAQR
                     HLRDRYPELIPQAGKTARAPVAYFHGCAANYFDDGVGDAVIAVLRKHGVEPALPPQRC
                     SGTPIETYGHRALAKEGARVNLASMAGYDKVVTGCASCTLMLKDYPTLFAGEPEQAAA
                     QALAKRVTHISEFVAQSSVKPAMAGSQSAKCKVAYHSSCHLRAAGVTKEPRAILAGLP
                     GVDYVEMPDADRCAGGAGTYLVKDFDTSQRIVERKRRAVEQSGAKVVATSCPACMIQL
                     RTGLQGAAEVKHVAQLIQEAYEAADRS"
     gene            5178..5456
                     /locus_tag="VLL06_07215"
     CDS             5178..5456
                     /locus_tag="VLL06_07215"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HSS30790.1"
                     /translation="MVTVLVFGLTLRDALGESELELEVSAPTTVRKLIQSNQDRMGPL
                     LQFLTNREVMITVNKKVSAEDTVVKDGDIVKLSHQSRVSYDGTRDIPI"
     gene            complement(5774..>6360)
                     /locus_tag="VLL06_07220"
     CDS             complement(5774..>6360)
                     /locus_tag="VLL06_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30791.1"
                     /translation="PLAWANPEDALSPPTVRAPFDEKDEATLQTEVTKQALTFLQRVE
                     AHWGQQPEALRLSMAGTVERDGEGKLIYLRNLADHGVLEGYEFHHESLVRGQYILLQR
                     PLNGLNEFIGYYRTLKEQLTTTYGSPTNDQTIWDNDLYKPVPDYWGVAVLIGHLHYRA
                     TWETADGTITLELTGNQHSRLFLEYRSHNEAART"
BASE COUNT         1330 a         1780 c         1869 g         1381 t
ORIGIN      
        1 gtactggatt ctcatggtcg aatgcgaagc ctgacgagct ctggacggcg cgtgcgcaag
       61 aaccagattt gcccaaaccc attgagcctg tcctctcaac acacgatcag ttccagagct
      121 atatgaaggc cggccagcag gctgaggcag aagaatggct cagtcgcttg gttacggcac
      181 agccggatga tgcggaggcc cgagaactct tcggctccct gcttgaaacc aagggcgata
      241 ccgccggagc agcgttgcaa tattcccgcg ccttggaact gctcctggca tatccccagg
      301 atgagaactc gacgcggccg gcgagtctct atgcgaaagt caaagaactc gctccggcca
      361 gtccgttgct ggccaaatgg gcctcgacgt tttcgccgac gccacaggcg ccggtctctg
      421 ccgccgagac gccggtttct gctccacccg tgcacgagat caatccggag acccactaca
      481 cgctgggagt ggcctataag aatatggggc tgctggacga agcgaaggaa gagttttgcc
      541 tttcgctgca gagccaggac tttttctcgg actcctgtct catgatcgcc gtctgtttaa
      601 aagaacaggg agaatgccgg tcggcgagtg cacagctcga gttgctgctg aaggatccaa
      661 actgccaggg ggccaaggcg ctggccattc gatatgaact cggcttactc tacgaagccc
      721 aggaacaatg gagccaggct gccgcaatct acgaatcgat tccgacgttc cacgacgtac
      781 cgaagcgact cgaatcagct cgtgcacacg aggccacagg tgagcccacg tcggccttcc
      841 gctacggaaa ctagccgcag ggtgctgaaa aagtccgcca tctcacccgc ccagccctgg
      901 cgcgccgaga cgcgcctttt cccatgcttc gttctcacct tgaaagcatc ctcaacgtag
      961 cccatccggg aaagaactgc gctagcagtt cggggagggg ggtgagaatg ttacgcctcc
     1021 ggtgctttca tcggctgcgg ccttgctggc gaactttttg agcatcctgc agtccgtctc
     1081 cttcctagtg aatctggttt ggggcgggac gtccttccaa gacagcttga atattctcca
     1141 aacagaccat tcccatctga accctcgcat gtagggtggc cgagccgaga tgcgggagca
     1201 gcaccacctg tttcaactgt gccagtgttg agtggatggc cggctcctgt tcatagacat
     1261 cgagtcctgc gccggcaatg attccagtgt tgagagcgtc gaccaacgcg gcttcatcga
     1321 caatcggacc acgggccgta ttgatgacga aggcagtggg tcgcatccag gccaattcgc
     1381 gcgtaccgat cagatggtgt gtagcctgcg taaggggcac atgaattgtc acgatatcgg
     1441 cctccccgag gatatcctgc agcgatcgat actcccactc actaggtaac gatgaaacgg
     1501 tctgaggttg gcgtgtgtga taacgcaccg gcatgcgaaa ccctatcgag cgctgggcca
     1561 ccgcttgccc gatccgtccc atcccgataa ttcccagtgt cttgcccgtg acttccgcac
     1621 tcaagagttg cgtgggactc caccccgtcc aagtgccaga ccgaacgagg gcgtcgcctt
     1681 cgacgacgcg gcgtgccgtt gccaggatca aggcccaagt cagatccgcg gtcgcatccg
     1741 tcaggacatc cggagtattg gtgacgatca gaccacactg tcgcgctgtg gcaagatcga
     1801 tgttgttata ccccacggca tagttcgcca gaattttcag ccgggtggcc gcatgaatag
     1861 tctcggcgtc aatgcgatcg cccagggtca ggatcgcggc atcggcttgg ccgagcccct
     1921 cacgcaatgt cgccggcgag ggcaacgcat cgctgggacc ctgtatcagt tgaaaccgtt
     1981 cccgcacgat aaccatcact ggatcgggga ggaggcgtga gacataaaga gtgggacgtg
     2041 tgtcggtcat aggcctgtgt tcctatccgc gcggatcata gcaggatgct gaaaaattcc
     2101 gccagcagaa gaagaccgtt atttggtttg tctggtctat ctcgttattc tggttctttg
     2161 cttgaacgaa accaactaga tgaaccaaat caaccagaca aaccagactt gtgctcgtct
     2221 taatggaccg tctagaatga acgacgcctt caactatgac cgcacccact ctcattctgc
     2281 cagaacgttc ccgcgccgta gcctcagatc ttcgcgccgc aattggcgcg cataaagtca
     2341 aagacgatta ctccacgctc actgcctatg cggtggatgc cagcatctat cgggtggagc
     2401 ccaaggcggt tgtgcttgtc gagtccgagg aagacattgc ggcgacggtg cgttacgccg
     2461 tcgcgcgagg tattccactg acgcctcgtg ctgccgggac gaatttgacc ggctcggcga
     2521 tcgggtcggg gatcattctg gacgtgtcgc gcttgaatcg ggtgctggag ctgaatcgag
     2581 aagagcgttg ggctcgggtg cagccgggga tcgtgctcgc cgagttgaat aaaggattgg
     2641 ggcggcatgg tctattgttt gggccggatc cctccagcgg cgacatgtgc aagctcggtg
     2701 ggatgttggc gaacaactcg tccggtcccc acacgctgcg gtatggcgcg gtcaaagata
     2761 atgtacaggc gcttcgtgtc tgcctgcagt ccggtgactg gctggatgcg aaggcctatg
     2821 ggctcgacga ttcacgatgc acggatctgc tgaaggcgca caggccgctt catgatctgc
     2881 ggtcgttgct ccaagatcat gctgcgctga ttgcgcagaa acgtccgacg gtcagcaaga
     2941 acagttgcgg ctataatctt ttcggtcttg ccgatgggct tgcgcgaggc tgcttcgatc
     3001 ttcctaaact cttcgtgggc agcgagggca cgctcggtct cttcagcgag gcgacgatta
     3061 gactggtcga gaaaccccgc gcgaccctca cggcgctcat ccactttcac cgtttggagg
     3121 atgtgggcga cgcggtgcca aaactgttgg agttaagtcc cagtgccttg gaagtgatgg
     3181 atgccaacac gctcaacctg atcggacggg cgaagcacgg cattccggca gacgcggccg
     3241 ccactctttt ggtagaactg gacggcgacg cgataggaag cgatctccga gcacaggcgg
     3301 aacagatgat cgacatctcc cgccggtatc ccctcacctc cgatcccgtg atcgccttcg
     3361 atcccgagca acgggagcaa ttgtggaagg ctcgcaaggc cctctatccc acactctatc
     3421 gattcgatcc caagaagaag ccgatcaact ttgtggacga cgtggtggtg ccggctgaac
     3481 gaatcagcga actcatccgc tatctggaag acttctttgc cgggcagcac gtgccggtcg
     3541 cgattttcgg tcatattggc aacgggaacg cccatatcat tcccttgctc gatgtgaacg
     3601 accacgacga tttccagaag atggtggagg gctatcacga gatacaccaa acggtcttag
     3661 gccgcttcgg cggttccatc tgtggggagc atggggatgg ccgggtgcgc gccgaatatg
     3721 tgaaggcgat gttcggcccg gagctgtacg atctgtttgt ccgtgtgaag cagagctttg
     3781 atcccaccgg agtgctcaac cccggcatca agatcagcga ccggcccttc acagaccata
     3841 tcgatcatgt gagattgtca aaatcctgtg cgacctgcgc caagtgtaac gccgtctgtc
     3901 cggtctacga tgtgtttcaa tcggaagaca tgagttcgcg tggttggttc gaaattgtca
     3961 cggacaagaa ctatagctat ctgaattcga agcgagttgt ggaagcctgt ctcaactgta
     4021 agtcctgccg tacagcctgt ccggcgggag tcgacgtgtc acaactcatt ctggaccgac
     4081 gtgctgagaa cccgaacgag atggcaggtg tcatatttcg tttccatgca cagaccgcac
     4141 gatttgagca gttcttaaag tttctcgcga ggtatcagtc gatatgggat cgaccgctga
     4201 ttcggcggtt actcgatcgg ctaaccaagc cgtttatgca tcgtcttgcc gaaacagcac
     4261 gattgtcgcc tcaactggtg ttgcctcgac tggcccagag gcatctccgt gaccgctatc
     4321 cagaactgat tccccaggca gggaaaactg cccgcgctcc ggtggcctat ttccatggct
     4381 gcgccgccaa ctactttgac gatggagtgg gtgatgccgt gatcgccgtg ctgcgaaagc
     4441 atggggtcga gccggctctg ccgccgcaac gctgttccgg gacgccgatc gaaacatacg
     4501 ggcatcgcgc gttggccaaa gaaggggcac gggtcaatct cgcgtccatg gcaggatatg
     4561 acaaggtggt gaccggatgt gcctcctgca ccctcatgtt gaaggactac ccaaccctat
     4621 ttgccggtga gccggagcaa gcggcggccc aggccttggc caagcgggtc acacacattt
     4681 ctgaatttgt ggcacagtcg tcggtgaaac cagctatggc tgggtcgcag tccgcgaagt
     4741 gcaaggtggc ctatcattcg tcctgtcatc ttcgagccgc gggagtgacc aaggagcctc
     4801 gtgcgattct cgcaggctta ccaggcgtcg actatgtcga gatgccggat gccgaccgct
     4861 gtgccggtgg agcagggacc tatctcgtga aggattttga cacgtcgcag cgtattgtcg
     4921 agcggaagcg gcgggcggtc gaacagagcg gtgccaaagt tgtcgccacg agttgccccg
     4981 cctgtatgat tcaattgcgc acgggattgc agggcgcagc ggaggtcaaa cacgtggcgc
     5041 agctgattca agaagcctat gaggcagcag accggtcgta ggtgtatctg gtctatttgg
     5101 tttgtctggt tgatctggtt tgtggtgctc aaccaaacaa acgagacaaa ccaaacaaac
     5161 tacataacga ggttttaatg gtcacagtgc tggtttttgg actgacgctt cgagatgcgc
     5221 ttggtgaaag tgaactagaa cttgaggtat cggcgccgac cacagtcagg aagctgattc
     5281 aatcgaacca ggatcgtatg ggcccgctgt tgcagtttct gacgaatcga gaagtcatga
     5341 ttacggtcaa caagaaagtg agtgccgaag acacggtggt caaggatggc gatatcgtga
     5401 agttatccca tcagtcgagg gtctcctacg atggcacgcg agacattccc atctagccga
     5461 atgctgaaaa agccacttgt ctcacccgcc caacccctgc gcgccaagac gcgcatcttc
     5521 ccatgcttcg ttctcacctc gaacgcatcc tcaacgtagc caatcgggac agaactgcgc
     5581 tagcagttcg gggagggcgg gtgagaaagt tacgcctccg gtgctttctt cggctgcggc
     5641 ctcgctgcgg aacttttcga gcattcggtt gggctgcacg atctgcgaga ctgactcgac
     5701 gtggattgat tggacgccgt cctcggttta aatggctcgc tcatccttca tgtaccgccc
     5761 atcgcgttgg ctactacgta cgtgccgctt cgttatggga ccggtattcc aggaagagcc
     5821 ggctgtgctg attaccggtg agttctagcg tgatggttcc atctgcggtc tcccatgttg
     5881 cacggtagtg caggtggccg atgaggaccg cgacgcccca ataatcggga accggtttgt
     5941 agaggtcgtt gtcccagata gtctggtcgt tcgtcgggct tccgtatgta gtggtgagct
     6001 gttctttcag cgtccggtag tatccgataa attcattcag tccattgagg ggccgctgca
     6061 gcaggatgta ctggccgcga acgagtgatt catggtggaa ctcgtatcct tcaagcacac
     6121 catgatcggc gagattacgc aggtagatca gtttgccttc cccgtcgcgt tcgaccgttc
     6181 ctgccataga caggcgaagg gcttccggct gttgccccca atgagcctcc accctctgca
     6241 aaaatgtcag agcctgtttc gtcacctccg tctgtaatgt ggcttcgtct ttctcgtcga
     6301 atggggccct gactgtcgga ggcgagaggg catcctctgg gttagcccat gcgagagggg
//
LOCUS       DATIPF010000288         3749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1117407, whole genome shotgun sequence.
ACCESSION   DATIPF010000288 DATIPF010000000
VERSION     DATIPF010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117407"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VLL06_07225"
     CDS             <1..712
                     /locus_tag="VLL06_07225"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HSS30792.1"
                     /translation="AAGGTTLVAKRLLDAGILHAGQPTVTGRTIGEEAKDAKETSGQQ
                     VLRPLSNPIKQTGGLVILKGNLAPDGCVVKVAGHSIMTFRGPAKVYNREEDAFVAVQS
                     RQIKAGDVVVIRYEGPSGGPGMREMLGVTAAIVGAGLGDSVALLTDGRFSGATHGLMA
                     GHVAPEAIKGGPIGAVKNGDMIAFDIAKRTLSVELSKKEITSRLKKVKQPLPRYTSGV
                     MGKYARHVSSASEGAITT"
     gene            complement(896..1840)
                     /locus_tag="VLL06_07230"
     CDS             complement(896..1840)
                     /locus_tag="VLL06_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HSS30793.1"
                     /translation="MAAGTDLSPREIYEQDSSAVVMVMGYADGGKKGSGGTGSIIQSD
                     GLVLTNAHVAIEEQTGKPFARLFVFLKPARVTGESKSDLSRMVRAKVVAYSQPLDLAL
                     LKLDSVTESLPVINVSESGRVRIGDRVVAIGHPEQGGLWTLTTGVISAEVDNFNGVKG
                     KHVFQTETGLNRGNSGGPLLDGQGQMIGVNTAIARVASDGLPITSISFSLKSSVAAQW
                     LREQGVGGRAGRETPLPTGQMEPAPDRPLAPSVPPQGKPIQPPQSDLSLRPTMKPEVV
                     QPVPARPYNLDQLISDRAKAEADLDSMMSEMRGRMKGR"
     gene            complement(2251..3372)
                     /locus_tag="VLL06_07235"
     CDS             complement(2251..3372)
                     /locus_tag="VLL06_07235"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HSS30794.1"
                     /translation="MNIPLLDLKAQFQPLRAEILAAMQAVCDEQGFVLGPRVVAFEES
                     IAQYIGSRYAIGCASGSDALLLSLMAMGVQAGDEVVTIPFTFFATASAVSRLGAKPVF
                     VDIQADTFNIDPGVIEQAITPRTKAIIPVHLFGQCADMVAINEIAKRKRIRVIEDACQ
                     AIGAAQGGKRAGVLGDTGCFSFFPSKNLGGFGDGGLITTNDKALADSMAMLRVHGSQV
                     RYLHEAVGINSRLDALQAAVLQVKLKYLDQWTTGRRRNAERYQQMFARTKHADRVALP
                     PTQPGNFHVYNQFTVRAPKRDELRAFLKEKGVGTEVYYPLPMHLQNCFRDLGHQKGSF
                     PLSEQAAEEVMSLPIYPELTEAQQIYVVEMIAEFYRRTS"
     gene            complement(3469..>3749)
                     /locus_tag="VLL06_07240"
     CDS             complement(3469..>3749)
                     /locus_tag="VLL06_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30795.1"
                     /translation="LSTIFTLAMYVIGHLTADLKVFGAKMDELSRGILDGIYYLLPNL
                     ERFNLKGHVVHHLEVSGSDLTLIVAYGATYTAVVLFAASLIFHRRDFR"
BASE COUNT          857 a         1102 c         1010 g          780 t
ORIGIN      
        1 tgcagcagga ggaaccacgt tggttgccaa acgcctgctt gatgccggta ttttgcacgc
       61 cggccagccg accgtgaccg gccggacgat tggggaagag gccaaggacg ccaaggaaac
      121 gtccgggcaa caagtcctca gaccgctgtc gaatccgatc aagcagaccg gcgggctcgt
      181 cattttaaag ggaaaccttg ctccagacgg ctgcgtcgtg aaggtggccg gacattcgat
      241 catgacgttc cgtggtcctg cgaaggtcta caaccgggaa gaggacgcgt ttgtcgccgt
      301 gcaatcgcgt cagattaaag cgggcgatgt cgtggtcatt cgatatgaag gcccgtccgg
      361 cggacctggt atgcgcgaaa tgctcggcgt cactgcagcc atcgtcggcg cggggttggg
      421 cgactccgtc gcattgctca ccgacggccg cttctccggc gccacccatg gcctgatggc
      481 cggccatgtt gcaccggaag cgatcaaagg aggccccatc ggcgccgtga agaacggcga
      541 catgatcgcg tttgatatcg ccaagcggac gctgagcgtg gaactctcga agaaagagat
      601 tacatcccgc ctcaagaagg tcaaacaacc attgccacgc tacacctccg gcgtaatggg
      661 caaatacgcc cggcacgtgt cttcggcctc ggaaggggcc atcacgacat aaataggacg
      721 ctgaaaaagc ccgccagctc agaggctcaa cgtaccgaag cgtacgccac gcctcttcgc
      781 tcgcctcagg ctgttctggc accagcacca ttcgtaatat ttcagcaggg tattgcctcg
      841 agactgaaat tttaagcggc ctggcaggaa aaatgcgcaa gcgattttct cgattctatc
      901 tccccttcat cctcccacgc atctccgaca tcatgctgtc aaggtcggct tctgctttcg
      961 cacgatcact gatcaactga tcgagattat atgggcgagc cggaaccggc tgaaccacct
     1021 caggcttcat cgtcggcctg agcgacagat cagactgagg aggctggatt ggtttgccct
     1081 gtggcggaac tgatggagcc agtggtcggt cgggagcagg ctccatctgt ccagtgggta
     1141 atggtgtctc ccgtccagct cgtcccccca cgccttgctc ccgcagccat tgtgcggcaa
     1201 cactcgactt gagcgagaag ctgatgctcg taataggaag gccatccgag gccacacggg
     1261 caatcgccgt attgactccg atcatctgcc cttgtccatc caagagtggc ccacctgaat
     1321 ttccacggtt caatccggtc tcggtctgaa agacgtgctt gcctttcaca ccattaaagt
     1381 tatcgacttc ggcgctgatc acgccagtgg taagggtcca cagtccaccc tgttcgggat
     1441 gaccaatggc caccactcga tccccaatcc tgacccgccc cgattcactc acattgatga
     1501 cgggcagcga ttcggtgacg ctatcgagtt tcaatagggc aaggtccaac ggttgtgaat
     1561 aggccacgac tttggcacgt accatgcggg agagatcgct ctttgactct cctgtgactc
     1621 gagccggttt gagaaatacg aatagacggg cgaagggttt tccggtctgc tcttcgattg
     1681 caacgtgggc attcgtgaga accagtccgt ccgattgaat gatcgaaccg gtcccaccac
     1741 tccccttctt gccgccatcc gcatacccca tcaccatcac cacggctgaa gagtcttgtt
     1801 cgtagatttc acggggcgat aagtccgtac cggcagccac agtgctccct atcaaggaga
     1861 ttacgaacag tcccccccat atcattgacc tcatcatggc ctccctcttc tggctgctat
     1921 gccatccgtc acgttcatcg actgttgaac catgcatgca ctatacgacg gaatgcttga
     1981 gcggacaacg cattgacaga aacacgagcg tcctggaaag caggtgtgcg accgcatcag
     2041 ggcgagcaga ctgcgggaat gccattaaat acaacagaac ttggatggcc tgcgcggctg
     2101 ggctgtcgcg catgatagag gatcgaagct ggcgggacgc ggttgatctg cttagtctag
     2161 atcaaacaaa tagaccaaag aaacaagcga caccagcagg gctcctgccc ccgcgccacg
     2221 gtctgtgccg caggaaaaca ttcccgtatc tcacgaggtg cgccgataga actccgcaat
     2281 catctcaaca acgtagatct gctgcgcttc tgtcaattcg ggatagatcg gaagagacat
     2341 cacttcttca gccgcctgtt ctgacagggg gaagcttccc ttctgatgtc ctaaatcgcg
     2401 gaaacagttt tgaagatgca tggggagcgg atagtacact tccgtgccga cccctttctc
     2461 tttgaggaac gcgcgtaact catcgcgttt cggcgcgcgc acggtgaatt gattgtagac
     2521 gtgaaaattg ccaggctgtg ttggtggaag cgcgacgcga tccgcatgct tcgttcgggc
     2581 aaacatctgc tgataccgtt cggcattccg acgtcgcccc gtggtccact ggtcaagata
     2641 cttcagtttg acctgtaata cggcagcctg cagcgcatcc agccggctat tgatccccac
     2701 cgcctcgtgg agatagcgga cctggcttcc gtgaacacgg agcatggcca tggaatcggc
     2761 aagggctttg tcattggtcg tgatgagccc tccatctccg aacccgccca aattcttgct
     2821 gggaaaaaaa ctaaagcacc ccgtgtctcc aagtacccct gcgcgcttcc ctccctgtgc
     2881 cgccccaatg gcttggcacg catcctcaat gacacgaatc cttttccgct tggcaatctc
     2941 gttgatcgca accatgtccg cacattggcc gaaaaggtgc acgggtataa tcgctttcgt
     3001 gcggggtgtg atcgcctgtt caatcacacc gggatcaatg ttgaaggtgt cagcctgaat
     3061 gtccacaaat accggcttcg cgcccaatcg tgacaccgcg ctcgccgtgg caaaaaacgt
     3121 aaatggaata gtgacgactt catcgccagc ctgcactccc atggccataa gagagagcag
     3181 cagcgcatcg cttcccgacg cgcaaccgat cgcatagcga gacccgatgt actgggcaat
     3241 ggactcctcg aatgccacaa cgcgtggccc caatacgaag ccttgttcgt cacagacggc
     3301 ctgcatcgct gccagaatct cagccctgag tggctggaat tgagctttga gatcaaggag
     3361 aggaatattc atggatcaaa gatcgccaca cgtgacacgc catccccatt gccgttcata
     3421 tgaatacctt ggccctagga agattgcaag cagagaatca gagtgaggct agcggaaatc
     3481 gcgccgatga aagataaggc tggccgcgaa gaggacgact gccgtgtacg tcgcaccgta
     3541 ggcgacgatc agcgtcaggt ctgagccact gacttcgagg tgatgcacca catgtccctt
     3601 caagttgaaa cgctcgaggt tcggcaagag ataatagatc ccgtccaata tcccccggct
     3661 aagctcgtcc atcttcgctc cgaatacttt cagatcggca gtaagatggc caatcacata
     3721 catcgctagc gtgaagatgg tgctcagag
//
LOCUS       DATIPF010000289         3493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1117429, whole genome shotgun sequence.
ACCESSION   DATIPF010000289 DATIPF010000000
VERSION     DATIPF010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117429"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..1250)
                     /locus_tag="VLL06_07245"
     CDS             complement(219..1250)
                     /locus_tag="VLL06_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HSS30796.1"
                     /translation="MIDLRSDTVTKPTEAMRQAMARADVGDDVYGEDPTVNRLQDMVA
                     TMFGKKAALFVPSGTMANQLAIRLHTQPGQEVIVESKSHIVRYEQGAAGALAGVQLHW
                     VVGARGLISAEQVEAAVRPNEPHTIQTALICLENTHNSGGGTIYPLATIERIRAVANT
                     HGIPMHLDGARLFNAIAATTLPPGSYAQHFETLSVCLSKGLGAPAGSLLMMNDLTLLD
                     KAKRLRRMYGGAMRQSGILAAAGIYALEHHIGRLKVDHDHAKQLARRLQQIPTVKINP
                     QDVDTNIVIFDVIGHRLTPPAIVAALKQEGVLINAIGGASFRAVTHLNISSAMTEEAC
                     EIFTRVLAN"
     gene            1590..2954
                     /locus_tag="VLL06_07250"
     CDS             1590..2954
                     /locus_tag="VLL06_07250"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HSS30797.1"
                     /translation="MHEVPVKLYIDKLLKECRDIARHLAMLPGPIKEKSLRAMADRLA
                     ADQEKILAANEKDVDAVGKSFEADVMKDRMKAAVARLRLTADDIKEMVEQLRLIADLP
                     DPVGAVTSRWERLGGLQVSRVRVPIGVIGVVSELSPLITVESIALCFKSGNLCVFRGA
                     PEWGLTQQAIGTSLREAAEQNGVPAGAWIIIERPEKEVAVELIRSGKSLDAIIPRGGA
                     GLRKAVIEQAKMPVLCYDGGLTHMYVDADTDMPMAQNVAINSKVQKAMAANSLDTLLV
                     QQVIGRQFLPPLINRMLVEFKIEVRACPKTLALMGQMAMTGHTSIIAAKEEDWRTQFQ
                     APVLAVKMVANLDEALAHIVAHGPCLTAVIATTNYESAMRFTREVDASAVFVNASARL
                     NAGESYGMGADIGLSGSRIHAKGPIGLEQLTCEKYVAFGSGQLRYPHPVPETYFDAIM
                     LKRP"
     gene            3095..3493
                     /locus_tag="VLL06_07255"
     CDS             3095..3493
                     /locus_tag="VLL06_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30798.1"
                     /translation="MMNDSLLQSLHSHLAEWGLRQFTSDESYFQWQRDTLSPSEITAL
                     HQRIEGKRSGSSVDEVSLYDAAAHPKILPQHFAHVMERTTRAAAGGRLDAQALHRQFT
                     GLAILDNGSAARIAHALLGHFPTWDRHTLC"
BASE COUNT          735 a          965 c         1038 g          755 t
ORIGIN      
        1 tcaacgacga tttccaattt gccattggtt aatcgccgga ccgcaccggg gctttctggg
       61 gacaagggaa tttcgaccca ctcgcggttc agtccgagcg aatcggtgac cccaaggatc
      121 cggctgatgt gatggaatga cacaacgtgt aacggcatgg ctgctccact atgcggcatt
      181 ctagatttca gtgggggagg tgtcaatgag cgaacgactt agttcgccaa cacacgtgta
      241 aagatctcgc aggcttcttc cgtcatggca ctggagatat tcagatgagt gacggcacga
      301 aaactggcgc cgccgatcgc attaatcaag accccctcct gcttcaatgc ggcgacgatc
      361 gccggaggag tcagacggtg gccgatgaca tcgaagatga cgatgttcgt gtcgacatct
      421 tgagggttga tcttgaccgt cggaatctgt tggagtcgcc gtgcgagctg tttcgcgtgg
      481 tcatggtcca ccttcaatcg cccgatgtga tgctcgagcg catagatgcc ggcggcagcg
      541 aggatgccgg actgccgcat cgcaccccca tacatgcggc gcagtcgctt ggccttatca
      601 aggagcgtca gatcattcat catgagaagc gagcctgccg gcgccccgag tcctttagag
      661 agacacaccg acaaggtttc aaaatgctgg gcgtaggagc ctggcggcaa ggtcgtcgcg
      721 gcgatggcat tgaacagccg tgcgccgtcc aaatgcatgg ggatgccatg ggtgttcgcc
      781 accgcgcgaa ttcgttcaat cgtggccagc ggataaatcg tcccgccgcc gctgttatgg
      841 gtgttctcaa ggcagatcag tgcggtctga atcgtatgcg gttcgttggg acggacagcc
      901 gcttcaactt gctcggctga aatcagaccc cgagcgccca cgacccaatg gagttggacg
      961 cctgccaggg ctccggcagc accttgctca tatcgtacga tatgggattt gctttcgacg
     1021 atgacctctt ggcctggttg cgtatggagg cgaatggcga gttgattggc catcgtgccg
     1081 gacggcacga agagtgccgc ctttttccca aacatcgtgg ccaccatgtc ctggaggcga
     1141 ttgacggtgg gatcttcgcc gtagacgtcg tctcccacgt ctgcccgtgc catcgcctgg
     1201 cgcatggcct cggtgggttt tgtcactgtg tcgctacgta ggtcgatcat cacacatcca
     1261 tgaggccgtc acacgatcgt gagcctcgcg cattctagca aattttctct gtgtgtggga
     1321 gatgtgctcc cggtgaggtg ccgcgtatca tgagatcctt cagagcttgc tcgcgtctct
     1381 tctagagagg gcagccagac ggtcattccc tgcatgcctg gagcgagcac cgttttattg
     1441 tggaggctct gcgagcagga atgatggccc ggttgtcccc tccctgcttt acgcgagaaa
     1501 aaactgcgtg ctatagtcgc gtcacgtccc cgagagagtt gttgttgaag acggttcacg
     1561 tgtcatcctg aacagatcac gggtgaataa tgcatgaagt tccagttaag ctctatattg
     1621 ataagctatt gaaagagtgt cgtgacatcg cgcgtcatct tgccatgtta ccaggcccga
     1681 tcaaagagaa atccttgcgg gcaatggcgg atcgattggc agctgatcaa gaaaagattc
     1741 tggcggcgaa cgaaaaggat gttgatgcag tcgggaagtc cttcgaagcc gacgtcatga
     1801 aagaccggat gaaggccgct gtggcccgct tgcgtctgac ggcggatgat atcaaggaaa
     1861 tggttgagca actccgcctg atcgcagatc tgccggatcc ggtcggtgca gtcacctcac
     1921 gatgggaacg gttgggcgga ttgcaagtca gccgagtccg cgtcccgatc ggggtgatcg
     1981 gcgtcgtgtc tgaactgagc ccgcttatta cggtggagtc gatcgcgctc tgcttcaaat
     2041 cgggtaacct gtgtgtcttc cgcggcgctc cggagtgggg actgacacag caggcaatcg
     2101 ggaccagcct gcgcgaagct gcggaacaga acggggtgcc agccggtgca tggatcatca
     2161 tcgaacgtcc ggaaaaagaa gtcgcggtcg aactgatccg gtcaggcaaa agcctcgatg
     2221 ccattattcc gcgtggtggg gcaggactcc gcaaagccgt cattgaacag gcgaagatgc
     2281 cggtgctctg ctacgacgga ggactcacgc acatgtatgt cgacgccgac accgacatgc
     2341 cgatggccca aaacgtggca attaattcca aggtacagaa agccatggca gcgaactcgc
     2401 tcgacacgct gctcgtgcaa caggtgattg ggcgacagtt cttgcctccc ttgatcaacc
     2461 ggatgctcgt agagtttaag atcgaggtac gggcctgtcc gaagaccctg gcccttatgg
     2521 ggcaaatggc catgaccggg catacctcga tcattgcggc aaaggaggag gattggcgta
     2581 cccaattcca ggcccccgtg ttggccgtca aaatggtcgc gaatttggac gaagccttgg
     2641 cacacattgt ggcgcatgga ccctgtctga ccgcggtcat tgccaccacg aattatgagt
     2701 cagccatgcg gtttactcgt gaagtggatg ccagcgcggt gttcgtcaac gcgtcagccc
     2761 gactcaatgc cggcgagagc tacgggatgg gcgccgacat cggactcagt ggttcacgga
     2821 tccatgccaa gggtccgatc ggtcttgaac aattgacttg tgaaaagtac gtcgccttcg
     2881 gctcgggcca actgcgctac ccgcatccgg tgccggagac gtatttcgac gcgatcatgc
     2941 tcaagagacc gtaatcgcct gctgaaaacg ctctccagct tcgttctcag tcgctcgaac
     3001 cactcaacgt accctcaccg cgtacgcctc gtggttcgca ctccttgcgg cctcgctgga
     3061 aaacgtgttg agcaggcgtg ccgaggctga atcaatgatg aacgactcat tgttgcaatc
     3121 gctccattcg cacttggccg agtggggcct ccggcaattt acgtcggatg aaagctattt
     3181 ccagtggcag agggacaccc tctcaccgtc tgagatcaca gcactgcatc agaggatcga
     3241 gggaaaacgt agcggatcct ctgtcgacga ggtctcgttg tacgatgctg cggcacatcc
     3301 caagattctg ccgcagcatt tcgcgcacgt catggaacgg acaacccgtg cggcggccgg
     3361 cggtcggctc gacgcgcaag cgctccaccg gcaattcacc ggtttggcta ttctcgacaa
     3421 cgggtctgcg gctcgtatcg ctcatgcttt actcggtcac tttcccacat gggatagaca
     3481 cactttatgt tag
//
LOCUS       DATIPF010000290         4759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_54464, whole genome shotgun sequence.
ACCESSION   DATIPF010000290 DATIPF010000000
VERSION     DATIPF010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54464"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VLL06_07260"
     CDS             complement(<1..820)
                     /locus_tag="VLL06_07260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HSS30799.1"
                     /translation="MQPFRVLRDLVRSFLRHGCASLAASLAFFSLVSLFPLVFLLLYG
                     LSFIVSHDVIGEQVLLNFLKGFLPALGEHVAAELHRVSALETVRWAVFLTFAWFGTLV
                     FYELDYALNVVFESTWRRHPLISTAIAVASLGMTGFVLILSYIATQTVNFLTGYAPRL
                     WGLDLLALAAHDLFLTYTLPFALAFLMVTGLYRLVPRRRPQWREAMIGALTFSLLWVA
                     AKLLFVTFSTYSNVYGDLYGSLLEVILLLLWVYYSAALLLIGAEVAHHLQQQAQV"
     gene            complement(906..2393)
                     /locus_tag="VLL06_07265"
     CDS             complement(906..2393)
                     /locus_tag="VLL06_07265"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HSS30800.1"
                     /translation="MSLYGADLLALLPELVVVVAACLVIALDPITPASKRDLLAWLSV
                     GALVLCLGLTGWQISMQNVRVSTFSDLVVIDAYARFWKILLYSVTGVTIVLSLPYLKV
                     ERIHLGEYYGFLLLALSGMMVMVSGVDLLTIYLGTELMSISLYILTGFNRSKPRSLEA
                     SAKYFVLGAFSSGILLYGISLLYGLAGSTKLISIAGAIGTRGTDDPLVLIATILLAVG
                     FGFKLAAVPFHLWTPDVYHGAPTSVTAFMAVAAKAASFAAFIRVFVEGLGGLKADWSV
                     LFLVISIITLVLGNVVAIVQTNIKRMLAYSSIVHAGYALIGFVAAGRGVGASGGTSGL
                     ASVMIYLAIYSFMTLGAFAVIGMLRKGGVEGEEIEDFTGLAKRQPLAAFLMLVFMVSL
                     AGIPPTAGFIGKFYVFMAAVEAGMAWLAAIALVFAAVSAYYYMRVVMVMYMRDPDPSS
                     VVPPRLVHSPALSFVLACAVAGVILFGIFPNPLVSFALQSVLTLK"
     gene            complement(2390..3949)
                     /locus_tag="VLL06_07270"
     CDS             complement(2390..3949)
                     /locus_tag="VLL06_07270"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HSS30801.1"
                     /translation="MTSSFPWLSLLLVLPLAGVVVLLFATESAARWIALVATLADLCV
                     ALPLWWLFDPQASQMQFTEHVAWITAPPIYYSLGLDGISLPLVLMTVGIMPLCVLISW
                     TAITTRVRSFMAMVLIMETAMVGVFVALDFVLFYVFWEAMLIPMYLLIGVWGGANRLY
                     AAIKFFLYTLVGSLLLLVAIFVLYYYGGHTFDILALSQGTYPASLQMWLFLAFFAAFA
                     VKVPMFPFHTWLPDAHVEAPTAGSVILASILLKMGTYGFLRFSLPMLPDASRAFTPVM
                     VVLSIIAIIYGAYMALAQVDLKKLIAYSSVSHMGFVTLGLFVLNQQGIEGAVLQMVNH
                     GITTGALFLCVGIIYERTHSRLIADNVGLAKPMPMYATLLVIFGLSSLGLPGTNSFVG
                     EFLVLVGTFYWSKIATAFASLGIILAATYLLWMLQRVVFGVPDPHHLPKLKDLNQREF
                     ATLVPLVLLIFWIGLYPNPLVSRMHQSVTNTIASMSRAKVAPTAHPSAGTTAPLLANN
                     GEQLKTQGPSR"
     gene            complement(4040..>4759)
                     /locus_tag="VLL06_07275"
     CDS             complement(4040..>4759)
                     /locus_tag="VLL06_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HSS30802.1"
                     /translation="LAGFPLTSGFFSKDHLLVSAWSSGPLGQVLTILGLVTALMTAFY
                     SFRLVFVTFWGPSHVDPHHADHVHEPSNTITLPLIVLAVLSIVTGYLGIPEFLGPMFE
                     SDIAEAAHAGGASIGIMMVATAMGLLGIAGAYYVYVRNPALPDQFARRWQSLYRASLN
                     KWYVDEAYDRTIVRPTFSGATELWKRVDVNVIDGAVNGVARAIAWGGWLLRFTQSGQA
                     QHYALGMALGAVVLLTMYLFL"
BASE COUNT         1230 a         1287 c         1435 g          807 t
ORIGIN      
        1 gcacttgcgc ctgttgttga agatggtggg cgacctcggc accgatcaat aagagtgcgg
       61 cggaatagta cacccataag agcagtagaa tgacttccaa gagggatccg tatagatcgc
      121 cgtatacatt cgaataggtg ctaaaggtca cgaataggag tttggctgcg acccacagca
      181 gactaaacgt gagtgccccg atcatcgcct cacgccattg cggccgccgc cgtgggacga
      241 gccgatagag tcccgtgacc atgaggaacg ccagcgcgaa ggggagcgta taggtgagaa
      301 acagatcgtg ggctgcaagg gccaagaggt cgaggcccca aagccttggc gcatagcccg
      361 ttaagaagtt gacagtttga gtcgcgatat aggagagaat caggacaaat ccggtcatac
      421 cgagcgaggc cacggcgatg gcggttgaga tcaggggatg gcggcgccac gtgctctcga
      481 atacgacatt taacgcatag tcgagttcat agaacaccaa cgtaccgaac caggcgaacg
      541 tcagaaacac ggcccacctg acggtttcca aggcactcac ccgatgtaat tctgccgcga
      601 catgttcccc gagcgctggg agaaacccct tgaggaaatt gaggaggacc tgttccccga
      661 tcacgtcatg actgacgata aagctcaggc cgtagagtaa gagaaagacg agggggaaga
      721 gggagaccaa cgagaagaag gcaagagagg cagccagact ggcgcatcca tggcgcagaa
      781 atgatcgtac gagatctcgg agaacacgaa agggttgcat agagcacgat gggtgaaggg
      841 cttgtcacca acgtagcacg agtcgccttc tcgtgcacct gttcagcgcc ttccacgtac
      901 aaagactatt tgagggtcaa gaccgattga agtgcgaagc tgacgagtgg gttgggaaag
      961 atgccgaaca gaatcacacc ggcgaccgca caggccaata cgaacgataa ggccggcgag
     1021 tgcacgagac gcggcggcac gacggaagag ggatccggat ctcgcatata catgaccatc
     1081 accacccgca tgtagtagta ggccgatacg gcggcaaaga ccagggcaat ggcagcgagc
     1141 caagccattc ctgcttccac tgccgccatg aacacataga atttaccgat gaagcctgcg
     1201 gtcggcggaa tcccggcgag cgaaaccatg aagaccagca tgagaaaggc tgcgagcggt
     1261 tggcgttttg cgagccccgt gaaatcttcg atttcttctc cctccacccc gcctttcctg
     1321 agcatgccga tgaccgcgaa ggcgccgagc gtcatgaacg aatagatggc gagataaatc
     1381 atgacgctcg cgaggccaga cgttcccccg gatgcgccaa ccccacggcc tgccgccaca
     1441 aaaccaatca aggcgtagcc tgcgtgcacg atgctggagt aagccagcat ccgtttgatg
     1501 ttggtctgca cgatggcaac gacattgccc aacaccaggg tgataatgga gatgaccagg
     1561 aacaggacag accaatccgc cttgagcccg ccgagtcctt cgacaaagac acggatgaat
     1621 gcggcaaaac tggcagcttt tgccgccact gccatgaacg ccgtgaccga ggttggggcg
     1681 ccgtgataga cgtcgggcgt ccacaaatgg aatggcacgg cggcgagctt gaaaccgaat
     1741 ccgacggcaa gcaatatcgt cgcgatcagc acgagggggt catccgtccc gcgagtcccg
     1801 atggcgccgg caatcgatat cagcttggtg ctaccggcca gtccgtagag caacgagatg
     1861 ccgtacaaca gaattcccga ggagaaggcg cccagaacga agtatttggc tgaggcctcc
     1921 agcgaacgag gcttagaccg gttgaaaccg gtcaatatat agagcgagat ggacatgagc
     1981 tcggtgccga gataaatcgt gagcaagtcg acaccggaga ccatgaccat catgccggag
     2041 agggcgagga gaaggaaccc ataatattcg ccgaggtgta tgcgctccac cttcagatag
     2101 ggcaacgaca ggacgatggt cacgccggtc acgctgtaga gcagaatttt ccaaaacctg
     2161 gcataggcat cgatgaccac caagtcgcta aatgtggaca cccggacatt ctgcatgctg
     2221 atctgccagc ctgtaagtcc aaggcagaga acaagggcgc ccacactgag ccaagccagg
     2281 agatctcgtt tcgaagccgg agtgatgggg tccaacgcga tgacgagaca ggccgcgacg
     2341 accacaacca gttcgggaag caaggccaag aggtctgcgc cgtatagact catcgcgatg
     2401 ggccctgggt ctttagctgc tctccgttgt tcgccagcag tggtgctgtc gtaccggcgg
     2461 aggggtgagc agtcggcgcg accttcgcgc gggacatgga ggcaatggtg ttggtcacgc
     2521 tctggtgcat ccgactcacg agtggattcg gatagagccc gatccaaaat atcaacagga
     2581 cgagtggcac cagcgtggcg aattcccgtt ggttgaggtc cttgagcttc gggagatggt
     2641 gcgggtctgg gaccccgaag accacacgct ggagcatcca gagaagatac gtggcggcga
     2701 gaatgatgcc caatgacgca aaggcggtcg cgatcttgct ccaatagaac gtaccgacga
     2761 gcacgaggaa ttccccgaca aaactattgg tgccggggag gccgagggag gacaagccga
     2821 aaatcaccaa cagggtggca tacattggca tcggcttggc caatccgaca ttatcggcga
     2881 tcaaccggct gtgggttcgc tcatagatga tgccgacgca gaggaataac gcgccggtcg
     2941 taatcccatg gttgaccatt tggaggacgg ctccttcgat gccctgttgg ttcaagacaa
     3001 acaggccaag ggtgacgaac cccatgtggc tgacgctgga ataggcgatc agtttcttaa
     3061 gatcaacttg ggccaacgcc atataggcac catagatgat cgcgatgatg gagaggacga
     3121 ccataacggg cgtgaacgcc cgactggcat ccggcagcat cggtaagctg aatcgcagaa
     3181 atccataggt gcccattttc aagaggatgc tggcaaggat gacgctgccc gcagtcgggg
     3241 cctcgacgtg ggcgtccgga agccacgtat gaaatggaaa catggggacc ttcaccgcga
     3301 acgcggcgaa gaacgccagg aagagccaca tctgtaggga agcagggtag gtgccttggc
     3361 tgagggcgag gatgtcgaat gtgtgccctc cgtaatagta caggacgaag atggcgacga
     3421 gcaggagcag acttcctacg agggtgtaca gaaagaactt gatggccgca tagagtctgt
     3481 tggcccctcc ccagaccccg atgaggaggt acattggaat cagcatggct tcccaaaata
     3541 catagaacag gacaaagtcc agcgcgacga agactcccac catggccgtt tccatgatga
     3601 gcaccatggc catgaagctc cgaactcttg tggtaatcgc agtccaggat atcaagacac
     3661 agaggggcat aatcccgacg gtcatcaaca cgagtgggag gctgattccg tcgagaccca
     3721 ggctgtaata aatgggaggg gcggtgatcc aggcaacatg ttcggtaaat tgcatctggc
     3781 tggcctgtgg gtcaaagagc caccagagcg gaagggcgac gcacagatcg gccaaggttg
     3841 cgaccagcgc aatccatcgc gcggccgatt ccgtcgcgaa caacagcacg acaactcccg
     3901 caaggggaag cacgaggagc agtgagagcc agggaaagga actggtcatc gattgttggt
     3961 caatagtcca aggtcatcgg cacgagaaca ggtgttcgtt gttcccatcg gcccatgacc
     4021 gctgaccatt ggcactcctt cacaagaata agtacatcgt caacaggacc actgccccca
     4081 gtgccatacc aagtgcataa tgttgcgcct gcccgctttg cgtgaacctc aagagccacc
     4141 ctccccaggc gatggcacgg gccactccat tcaccgcgcc atcgatcacg ttcacatcca
     4201 cacgcttcca cagttcagtt gcgccagaaa aggtcggacg gacgatcgtt cgatcgtagg
     4261 cctcatcgac ataccatttg ttcagtgagg cccgatacaa cgattgccac cgacgagcaa
     4321 attgatccgg gagcgccggg ttgcgcacat agacatagta ggccccggcg attccaagca
     4381 atcccatagc cgtggccacc atcatgatgc ctatggaggc tcctcccgcg tgagccgctt
     4441 cggctatgtc tgactcgaac atgggtccaa gaaactccgg aatgcccaga tagcctgtga
     4501 cgatgctgag cactgccaag acgatcagcg gcagagtgat ggtgttcgac ggttcgtgca
     4561 catggtctgc atgatgagga tcgacgtgcg aggggcccca gaacgtgacg aagaccagcc
     4621 ggaagctgta gaacgccgtc atcaacgccg tcacgagacc gagtatggtc aagacctgac
     4681 cgaggggtcc ggacgaccag gccgagacga gcagatggtc cttgctgaag aatcccgatg
     4741 tcagggggaa tcctgcgag
//
LOCUS       DATIPF010000291         3410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1206955, whole genome shotgun sequence.
ACCESSION   DATIPF010000291 DATIPF010000000
VERSION     DATIPF010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206955"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VLL06_07280"
     CDS             complement(<1..544)
                     /locus_tag="VLL06_07280"
                     /inference="COORDINATES: protein motif:HMM:NF019454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30803.1"
                     /translation="MDRIVECVPNFSEGRNRTTVCALVHAVESVPGVWLLDQTMDRDH
                     HRSVLSFAGEPAAVAEAAFRAIRVATDLIDLRKHKGVHPRVGATDVVPFVPLRGATMQ
                     DCIHLAKRLGQRVGTELEIPVFLYEQAALHRDHAPLESVRRGGLQGLAFRMASDPDWT
                     PDFGPPHLHKTAGALVIGARP"
     gene            complement(638..883)
                     /locus_tag="VLL06_07285"
     CDS             complement(638..883)
                     /locus_tag="VLL06_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HSS30804.1"
                     /translation="MIQADVKLDTLGYFCPMPIIMTLKKIKELTLGQVLEVVSDDEGI
                     KNDMPAWCDTTGHQMVGLEEEQTKSGTIYKAFVKKTK"
     gene            complement(962..1492)
                     /locus_tag="VLL06_07290"
     CDS             complement(962..1492)
                     /locus_tag="VLL06_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HSS30805.1"
                     /translation="MTTAQLDPAVALADLQISKPDKVTIVLLSGDMDRAMAAFIIATG
                     AAAMGMQVTVFFTFWGLNAIRKKGATSSAKDWLRQMFGLLNTGGADSLPLSRFHFWGL
                     GTKMMKQVMRQNRMPGVPELMETALGLGVHFIACTTTMGLMGITKDTLIEGIDQFAGV
                     TTYLAEAKQGSVNLFI"
     gene            1655..2743
                     /locus_tag="VLL06_07295"
     CDS             1655..2743
                     /locus_tag="VLL06_07295"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HSS30806.1"
                     /translation="MNSDLITEIKSSRNAPLLGFWYPACLSSELAPNSLKGTILLGLP
                     ILVCKTSQGAIAAMRDICPHRGMPLSFGKMKGDCVECAYHGWQFDQAGRCREIPALVE
                     NSPIRVDKIGITTYACRERDGYVWVFIPDPQQPNQITPDVPPLPLPSSPYRMIHISTL
                     LDCTIDDGIVGLMDPAHGPFVHQSSWWRSQASIHEKAKTFEPIPNGFRMVPHAPSTNS
                     GPYKLLNLFYGGPLTTTIDFVLPNQRFEFVQCGSLFVSSRATVTPISEQQCRIDFAAA
                     WNCLSWVPFSKSIFRYFANIFMTQDREAMERQAVGLRYKPALMLIDDADTPAKWYYKL
                     KAAHLASTQTGEPLDHPLKERVTLRWKS"
     gene            2758..>3410
                     /locus_tag="VLL06_07300"
     CDS             2758..>3410
                     /locus_tag="VLL06_07300"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HSS30807.1"
                     /translation="MTDGRATRKTPHLILLLSAILLCTLVSPLWSADSRRVVTVPALG
                     VLTGGQTGVVHYIVLQIDKDSRQEGPTVQFNEISLGGGSIVSEDWKEGVKHAVVAATK
                     TVGENGRGWLITIKNRSYNAWTEGMSASSAVAVGLVAAWRGDDIRSDVVLTGTIMPDG
                     QIEPVGSLVTKIEAAARAQFKTILVPRGQLDKADWDLSQLALRWNITVIEVATLEEA"
BASE COUNT          750 a         1028 c          873 g          759 t
ORIGIN      
        1 gaggtcgcgc gccgatcacc aaggcaccag ccgtcttgtg aaggtgagga ggaccaaaat
       61 ccggtgtcca gtccggatcg gaggccatgc ggaaggcgag gccttgaagc cctcctcgac
      121 gaaccgattc gagtggggca tgatcgcggt gcaatgccgc ctgttcgtag aggaataccg
      181 ggatctccaa ctccgttcct actcgttgcc ccaatcgctt cgccaagtgg atacaatcct
      241 gcatcgtcgc gccgcgcagc ggcacaaacg gcacaacatc cgttgctccc acccgcggat
      301 ggacgccttt gtgcttccga agatcgatga gatcggtcgc cacacgaatg gcgcgaaagg
      361 ccgcttcagc gacggcagcc ggttcgcccg caaaactcaa gacggagcgg tgatgatccc
      421 ggtccatcgt ctgatccaat agccagaccc ctggcacaga ttccacggca tgcaccaatg
      481 cgcacaccgt tgtccggttt cgcccctcac tgaagttcgg tacacattcg acgattcgat
      541 ccacgttcct cctcaccaag aatcaacaca acaaagccgg aggtctgatc atcatccctc
      601 cggcttcttt ctcttcactg aatccctgtc tgtgtaatta cttcgtcttt ttcacgaacg
      661 ctttatagat cgttcccgac ttcgtctgct cttcttcaag acccaccatc tgatggccgg
      721 tcgtgtcgca ccaggccggc atgtcattct taatgccttc atcgtctgag acgacttcaa
      781 ggacctggcc caaggtcagt tctttgatct tcttcaaggt catgataatc ggcatcgggc
      841 aaaaataccc gagcgtatcg agcttcacgt cggcttgaat catcgcaacc tgctgatctg
      901 gttagttggg gttattaggt ttcgccggtc ttgcattcat gaaccagtca gaccagatga
      961 actaaatgaa caaattcacg ctgccctgtt tggcctccgc aagataggtt gtcacgcccg
     1021 cgaactgatc gataccctcg atgagcgtat ctttcgtgat ccccatgagc cccatggtcg
     1081 tcgtgcaggc aataaagtga acaccaaggc ccagcgccgt ctccatgagc tccggcacac
     1141 ccggcatccg gttttgcctc atcacctgtt tcatcatctt cgtccccagc ccccagaaat
     1201 gaaaccggga gagaggaaga ctgtctgcgc cccctgtatt cagcaggccg aacatctgac
     1261 gaagccagtc tttcgcagaa ctggtcgcgc ctttcttgcg aatggcattc agaccccaga
     1321 atgtgaagaa gaccgtgacc tgcatcccca tggcggcagc ccctgtggca ataatgaacg
     1381 ccgccatcgc tcggtccata tcgccgctca acagcacgat cgtcactttg tccggcttgg
     1441 agatctgcag atctgccaac gcgaccgctg gatccagctg tgcggttgtc atgacgccct
     1501 ctgcccctcg ggtccgcata aatgtggcga tttcactggg gtttttacta tagtctccgc
     1561 ttctttttct tgtcaaggca atgaccttga ccccactcat tacgccggat atagttccag
     1621 acgaaccgat gtcacctgcc atcccattct tttcatgaac agcgacctca ttaccgaaat
     1681 caagtcatcc aggaatgcac cgctcttggg cttttggtat cccgcttgcc tgagcagcga
     1741 acttgcccct aacagtctga aagggactat tctgttgggc cttccgatcc tcgtttgcaa
     1801 gacatcgcaa ggtgcgattg cagccatgcg agacatctgc ccccatcgtg gcatgccgct
     1861 gtccttcgga aaaatgaagg gcgactgtgt cgaatgtgcc tatcatggat ggcagttcga
     1921 tcaggcaggc cgttgccgtg aaattcccgc gctggtcgaa aactccccca tccgtgtcga
     1981 caagatcggc atcactacct acgcctgccg tgagcgggat ggttatgtgt gggtgttcat
     2041 tcccgaccct caacaaccga accaaatcac cccggatgtg ccgccgctac cgctaccctc
     2101 gtctccctac cggatgattc acatctcgac gctattggat tgcactatcg acgacggcat
     2161 cgtcggcctg atggacccgg ctcacggccc ctttgtgcat caaagctcct ggtggcgcag
     2221 ccaggcgagc atccatgaaa aggccaagac ctttgagccg attcccaacg gctttcgcat
     2281 ggttccacat gctccctcaa caaacagcgg accgtacaaa ctcttgaacc tgttttacgg
     2341 cggaccgctg acgacgacca tcgactttgt actgcccaac cagcgattcg aattcgtcca
     2401 atgtgggtcg ctcttcgtgt ccagccgcgc cacggtcacg ccgatcagtg aacagcaatg
     2461 ccgcatcgac tttgccgcgg cgtggaactg cctcagttgg gttccatttt ccaagagtat
     2521 ctttcgttac ttcgccaaca ttttcatgac gcaggaccga gaggcgatgg aacgccaggc
     2581 ggtggggtta cgctacaaac cggctttgat gctcatcgat gacgccgata cgccggcgaa
     2641 atggtactac aagttaaaag ccgcgcatct tgcatcgacg caaaccggag agccactcga
     2701 tcatccgctc aaagaaagag tgacgcttcg ctggaagagt taattgcgtg taacatcatg
     2761 acggacggca gggcgactcg gaaaacacct caccttattc tgcttctctc ggcgatcctt
     2821 ctttgcacat tggtttctcc gctctggtct gcagacagcc gtcgtgttgt cacggtgccg
     2881 gcactgggtg tgctcaccgg tgggcagacc ggagtcgtcc actacatcgt cctccaaatc
     2941 gacaaagatt cccgacaaga gggaccaact gtccaattca atgagattag cctgggtggt
     3001 ggctcaatcg tcagtgagga ctggaaagaa ggtgtcaagc acgccgtcgt ggctgccaca
     3061 aagacagttg gagagaacgg acgagggtgg ctaatcacga tcaagaaccg gtcatacaat
     3121 gcgtggaccg agggaatgag tgccagcagc gctgttgcgg tcggcctcgt tgcagcctgg
     3181 agaggcgacg acatcaggtc ggatgtcgtc ttgactggaa ccatcatgcc ggacggtcag
     3241 atcgaaccgg tgggttccct ggttactaaa atcgaagcgg cagcccgtgc acagttcaaa
     3301 actattctgg tccctcgcgg gcaactagac aaagctgact gggatctctc ccagctggca
     3361 ctccgatgga acatcaccgt cattgaagtg gccacgctgg aagaggccta
//
LOCUS       DATIPF010000292         3788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_55133, whole genome shotgun sequence.
ACCESSION   DATIPF010000292 DATIPF010000000
VERSION     DATIPF010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55133"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VLL06_07305"
     CDS             complement(<1..417)
                     /locus_tag="VLL06_07305"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HSS30808.1"
                     /translation="MIPLSTLLPTYATINLAALAHNLSRITHYLSPGCETMAVVKANA
                     YGHGAVETAQALARLGIGRFAVASLAEGIELRQAGLSTPIIVLGALFEEQVSDLVAHQ
                     LTPVVSDGRILPTLVKAALSQQTPYPIHLKVETGMGR"
     gene            complement(414..977)
                     /gene="frr"
                     /locus_tag="VLL06_07310"
     CDS             complement(414..977)
                     /gene="frr"
                     /locus_tag="VLL06_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HSS30809.1"
                     /translation="MSNPAAIHHRVTEKMESALEHLKRDLASLRTGRASVALFDGVRV
                     DYYGTMTPLKQVANISTPEARLITIQPWEPSLIKEIEKSLSGSGLGVTPSNDGKIIRI
                     PLPPLTEERRKDLSKICKKHGEDTKVVVRGFRRDANEELKKLQKDAKLTEDELRKGEA
                     DTQKLTDLYIHKIDDVVKKKEHEIMEV"
     gene            complement(996..1721)
                     /gene="pyrH"
                     /locus_tag="VLL06_07315"
     CDS             complement(996..1721)
                     /gene="pyrH"
                     /locus_tag="VLL06_07315"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HSS30810.1"
                     /translation="MNPAKYRRILLKVSGEMLAGEQGYGIQPSILEGLASEIASVVAL
                     DIEVAVVIGGGNIFRGIAASASGMERASADYMGMLATVLNALALQNALERAGIMTRVQ
                     SAIEMRQLAEGYIRRRAIRHLEKKRVVIFAGGTGNPYFSTDTAAALRAMEIGAQVIMK
                     GTKVDGIYDADPVKNPSAKKYQTISFLSILNQNLQVMDATAISLCMDNKLPLIVFNLK
                     EFGNFKRVATGEEIGTMVIPDRR"
     gene            complement(1718..2314)
                     /gene="tsf"
                     /locus_tag="VLL06_07320"
     CDS             complement(1718..2314)
                     /gene="tsf"
                     /locus_tag="VLL06_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HSS30811.1"
                     /translation="MAGSSQLVKELREKTGAGILDCQKALTENNDDVEKAIDYLRQKG
                     LAAALKKSGRETNQGLVHAYIHMGGKIGVLIEVNCETDFVARNEEFKTFVNDLALQVA
                     AGKPSFVKREDIPTSLIEKERTIYEGQAKEMGKPPAAWPKIVEGKLEKFFQESCLIEQ
                     SFVKDPAITIKDLLALKISKIGENMNIRRFTRYQLGEA"
     gene            complement(2375..3148)
                     /gene="rpsB"
                     /locus_tag="VLL06_07325"
     CDS             complement(2375..3148)
                     /gene="rpsB"
                     /locus_tag="VLL06_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HSS30812.1"
                     /translation="MGVVSIKELLEAGVHFGHQTNRWNPKMKKFLFGERNGIYIIDLQ
                     QTLARMEQAYAFVRDTVAGGQSVLFVGTKRQAAEILQEEALRANMFFVNQRWLGGMLT
                     NFQTIRKSIDKMKKLEAKLADPSQYGLKKKEVMKMQKEIVKLQKYLSGIKDMRGVPGA
                     VFVLDTRIEHIAVQEANRLEIPVIAILDSNCNPDSITFPIPGNDDAIRSIKLITSRIA
                     DACIEGAHLRTQREEAEFKPIPPVGDKTAAVSFASAPAS"
     gene            3424..3687
                     /locus_tag="VLL06_07330"
     CDS             3424..3687
                     /locus_tag="VLL06_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSS30813.1"
                     /translation="MPNILLLVSPSCGACPSAKSLWKQLRVKYSFSYREVDITTEDGR
                     SLADRHSVRAVPATIIDGRLTFVGVPSRESAEKALMLKMKPRE"
BASE COUNT          741 a          993 c         1099 g          955 t
ORIGIN      
        1 ccgacccatc cctgtttcaa ccttgaggtg gattggatag ggagtctgct gcgagagagc
       61 ggctttgacc aaggtgggaa ggatccggcc atcgctgacg accggggtca gctggtgagc
      121 gaccaggtcc gagacctgtt cctcaaagag cgcaccgagc acgataattg gagtcgaaag
      181 gccggcttgc cggagttcga tgccttccgc aagcgaagcc acggcaaaac gcccgatgcc
      241 aaggcgggca agcgcctgag cggtttccac cgctccatgc ccgtaggcat tggccttgac
      301 gaccgccatc gtctcacagc ccggtgacag gtagtgcgtg atgcgggaga gattatgggc
      361 gagggccgcg agattgatgg tggcgtacgt gggtaacaag gtcgagagtg ggatcacact
      421 tccataattt cgtgttcttt tttcttaacc acgtcgtcga ttttgtgaat gtacaggtcg
      481 gtgagtttct gcgtgtcggc ttcgcccttt cgcagctcgt cttcagtgag cttggcatct
      541 ttttgcagtt tcttcaactc ttcgttcgca tctcgacgaa acccacgcac gacgactttc
      601 gtgtcctcgc catgcttttt acagatcttg ctcagatctt tgcgccgttc ttcggtcagg
      661 ggaggaaggg gaatgcgaat gatctttccg tcgttggatg gggtgacgcc gaggcccgat
      721 ccggacagtg atttttcgat ttccttgatg aggcttggct cccagggctg gattgtgatg
      781 aggcgggcct ccggagtcga aatatttgct acctgcttca gcggggtcat cgtgccgtag
      841 taatcgactc gaacgccgtc gaagagggcg accgatgctc gcccggttcg taggcttgcc
      901 aaatcgcgct tgagatgctc cagcgcggac tccatcttct ccgtaactct gtggtgtatg
      961 gcagcagggt tggacatgag agactccctg gaaagtcagc gtcgatcagg tatgaccatg
     1021 gttccgatct cctcgcccgt ggcaacccgt ttgaaattac cgaattcctt caggttgaag
     1081 acgatgagcg gaagtttgtt atccatgcag aggctgatgg ctgtggcatc cattacttgc
     1141 aaattctgat tcaaaatcga gagaaaggag atcgtctgat atttcttggc tgagggattc
     1201 ttcacagggt cggcgtcgta gatgccgtcg accttggtcc ctttcatgat gacctgggcg
     1261 ccgatctcca tcgccctgag tgccgccgcg gtatcggtcg agaagtaggg attccccgtg
     1321 ccgccggcga agatgaccac gcgttttttt tcgagatggc gaatggctcg ccggcggata
     1381 tacccctctg ccagctggcg catttcgatc gcggattgga cccgcgtcat aatgccggcg
     1441 cgttcgagcg cgttttgcag ggctaaggca ttgagtacgg tggcgagcat ccccatatag
     1501 tcggcggagg ctcgttccat gccggacgcg ctggcggcaa taccgcggaa gatgttgccg
     1561 ccgccgatga ccacggcgac ttcgatgtcg agtgccacga ctgaggcaat ttccgaggcg
     1621 aggccttcaa ggatagaggg ttggataccg tagccctgct caccggccaa catctctccg
     1681 ctcactttga ggaggatgcg ccgatacttg gctgggttca tgcttcgcct aattggtacc
     1741 gggtaaagcg gcggatgttc atgttctcgc cgattttgga gatcttcagg gcgaggagat
     1801 ctttgatagt gatggctggg tctttgacga aggattgttc gatcagacag ctctcctgga
     1861 agaacttttc caatttacct tcgacgattt ttggccatgc ggccgggggt ttacccattt
     1921 ctttggcctg tccctcataa atcgttcgtt ctttctcgat gagcgaggtg ggaatatcct
     1981 cccgtttgac aaaggagggt tttccggcag cgacctggag ggccaggtcg ttaacgaacg
     2041 tcttaaactc ttcgttccgg gcgacgaagt cggtttcgca gttgacttcg atgagcacac
     2101 cgatttttcc acccatatgg atataggcgt gtacgaggcc ttgattagtc tcgcggccgg
     2161 atttcttgag tgcagcggcc aagccttttt ggcgcaagta atcgatggct ttctcaacat
     2221 cgtcgttatt ttcggtgagc gctttctggc aatcgaggat gccggctccg gtcttttctc
     2281 tcagttcctt aacaagctga cttgatccag ccatacagta ctatccttct cgctaagcag
     2341 tgagtgtggt cgttgatccc tgcgatgccg tgcgttatga agccggtgcg ctcgcgaagc
     2401 tgacggccgc ggtcttatct cctactggag gaatgggttt gaattccgct tcttctcgtt
     2461 gtgtccgtag atgggcccct tctatgcagg catcggcaat gcgagacgtg atgagcttga
     2521 tggagcgaat ggcatcgtcg tttccaggga tggggaaggt gatggagtca ggattgcagt
     2581 ttgagtcaag gatcgcgatc acagggatct ccaaccggtt tgcttcctgc acagcaatgt
     2641 gttcgatgcg agtgtccagc acgaatacgg cacctggaac cccgcgcatg tccttgatcc
     2701 cgctcagata cttctgaagc ttgacgatct ccttctgcat cttcatgact tcttttttct
     2761 tcagtccgta ttggctggga tctgccagct tggcctccaa ctttttcatt ttatcgatgc
     2821 tcttccggat ggtctggaag ttcgtaagca ttccgccgag ccaacgttga ttgacgaaga
     2881 acatgttggc acgtaaggct tcttcttgaa ggatctcggc ggcctgccgc ttggtcccga
     2941 caaacaggac cgattggccg cccgcaacgg tgtcgcggac gaaggcatag gcttgttcca
     3001 tacgggccag tgtttgttga agatcgatga tatagatgcc gttgcgttct ccaaagagga
     3061 acttcttcat cttggggttc catcgattcg tctggtgccc gaaatggaca cctgcctcta
     3121 acaattcctt gatcgaaacc actcccatgc cttcacctcc cctcaagact tgcagagcac
     3181 ctactgacgc aggcgagggg gtgtggcccc cttattctca agtcgagcga cgtccgtgac
     3241 gccgatatga attttatata tgtccttcac gtacgagatt atacgagaag gaggcggtct
     3301 cctgaccctg ttacagcctt ggcacgctag catgatgacg gtcagtttgc aagaccagcg
     3361 ccccctcgct tgccgcccta ttcaacccgg tgttaagatc ctttggctat ttggaggatg
     3421 cctgtgccga acattttact gttagtgtcg ccgagctgtg gcgcctgccc atcggcgaag
     3481 agtttgtgga aacagttgcg ggtgaaatat agcttcagct accgcgaggt ggatatcacg
     3541 acagaggatg gacggagcct cgccgatcgg cattcggtca gggcggtgcc ggccaccatt
     3601 atcgatgggc gactgacgtt cgtcggcgtg ccgagccggg agagcgcaga gaaggccctc
     3661 atgctcaaaa tgaaaccccg cgagtaaggg aaggaccgct ctaatggatg aagatgaagg
     3721 ttgtgaactc cctgaactcc ctgttatcga caagggacct ccgccggact gtcccatgtg
     3781 cggagacc
//
LOCUS       DATIPF010000293         2250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_588156, whole genome shotgun sequence.
ACCESSION   DATIPF010000293 DATIPF010000000
VERSION     DATIPF010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588156"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VLL06_07335"
     CDS             <1..287
                     /locus_tag="VLL06_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HSS30814.1"
                     /translation="GDTFALRVKGESMKDDGILPGDLVVVRKQKTARNGQTVVALVNH
                     EATIKTYFKKDSHIELHPANTTMQPIIVQPSDTFHIEGILIGVIRHCMVL"
     gene            303..554
                     /locus_tag="VLL06_07340"
     CDS             303..554
                     /locus_tag="VLL06_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30815.1"
                     /translation="MMLTASKSVEADRLVDLERTVDSLNGQLREMQWELYRAKAEPLS
                     MRLWRNLVSPKGRREFMSAVCNRRARPNTRMVVHADPRA"
     gene            complement(811..1284)
                     /locus_tag="VLL06_07345"
     CDS             complement(811..1284)
                     /locus_tag="VLL06_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30816.1"
                     /translation="MNERGVGGNGFVPCPVAAATHQMKPWSRAIAAILIMTVMGLTML
                     PAGVTFAQDQTSSSDDSDSTGIQVASWLLTIPYCAGKSAFAVAGSVVGSLGYAFSGGN
                     SETAQAIWTKSVYGTYILRPAHLRGEEPIHFVGQADEHKSESVKRAAASSNHADK"
     gene            complement(1307..1813)
                     /locus_tag="VLL06_07350"
     CDS             complement(1307..1813)
                     /locus_tag="VLL06_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30817.1"
                     /translation="MGISRRNRAGLVALVTGLGCLGLLWKHNVGAESSRIIDGSNVTY
                     FYQITDPSEAGLEVRKIGQFVQGRHQLPPIVERAVTGMKPGDKKNVELSGEDVFGPYD
                     AKKQTAVPKSDLPPGTKEGDVLADRAGREATVTRLSERFAVLDYNHPLAGKSLRMKIT
                     ILRVDDPS"
     gene            complement(1895..2221)
                     /locus_tag="VLL06_07355"
     CDS             complement(1895..2221)
                     /locus_tag="VLL06_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30818.1"
                     /translation="MKRTMFALTTIYLLWTAVVAVLTGEAATTFAGAIVGMDQAQRTI
                     TFQTLEGQTWALALSDSTMLKREQVAKGDRVRIEVDLTGKITKITKISEKPLSEPTQF
                     LDEVRP"
BASE COUNT          477 a          615 c          627 g          531 t
ORIGIN      
        1 gtggggacac gtttgcactc cgcgtcaaag gggaatcaat gaaagacgat ggcatcttgc
       61 caggcgattt ggttgtggtg cggaagcaga aaacggcaag aaatgggcaa acggtcgtgg
      121 cattggtcaa tcatgaagcc accatcaaga cctatttcaa aaaagactcg cacattgaat
      181 tgcatcctgc caacacgacg atgcagccga ttatcgtgca accgtcagac acgtttcata
      241 ttgaaggcat tctcatcggc gtgattcgtc attgcatggt cttatgaaaa gagaggaggg
      301 atatgatgct tacagcaagt aaatcggtag aggcagaccg gcttgtggat ttggaacgaa
      361 cggttgattc tttgaatggg cagttacgcg agatgcaatg ggagttgtac cgagccaagg
      421 cggagccgct atcgatgcgg ttgtggcgga atcttgtgag cccgaaaggt cgccgggagt
      481 ttatgtcagc tgtctgcaat cgacgcgcac gtccgaacac aaggatggtg gtccatgctg
      541 acccaagagc gtaggacgaa catgcgaccc gccggcacct gttccgactg tggaacgcct
      601 gcccagcggc ggactacatg tgtcgatgag atgactcatt caaccgtcga actgtgtgag
      661 cgctgttgct gtatctttcg acagaggcag gcgttcgcaa acggttgctg cgggtagaga
      721 gcacataggt taatccagat tgcgactgca atatcgcttg cccctcatgc tgcccaccgc
      781 aggctcaacg tgtacacacg gatttgctcc tcacttgtcc gcgtggttgg acgatgctgc
      841 ggcacgcttg acagactcgc ttttgtgctc atcagcctgg ccgacgaagt ggatcggttc
      901 ctcgcctctg aggtgggctg gccggaggat gtaggttcca tacacgcttt tggtccaaat
      961 cgcctgggcc gtttctgagt ttccccctga aaacgcgtat cccaagctac cgacgacact
     1021 gcctgcgacg gcgaatgcgc ttttgccggc acaataagga attgtcagca gccaactggc
     1081 tacttgtatc ccggtactat ccgaatcgtc agagctggac gtttgatctt gtgcgaaggt
     1141 cactccagcc gggagcatgg tcaagcccat gacggtcatg atcaggatag cggcaatggc
     1201 tctgctccat ggtttcattt gatgagttgc cgcggcgaca gggcatggta caaatccgtt
     1261 tcctcccaca cctcgttcgt tcatggcgca cctcctcatg gaatggtcac gatggatcat
     1321 ccactcgcaa gatcgtaatc ttcatgcgca gggactttcc ggccaacgga tgattgtaat
     1381 ccagcaccgc gaacctttct gacaaccggg tcacagttgc ctcccgaccg gcgcggtccg
     1441 cgaggacgtc tccttccttt gtcccgggtg gtagatcgct ctttgggacg gcagtctgtt
     1501 tcttcgcatc ataagggcca aagacgtctt cgcccgacag ttcgacgttc tttttgtcgc
     1561 cgggcttcat cccagtgacc gcccgttcca caatcggtgg cagttgatgt cgcccctgaa
     1621 cgaattggcc gatctttcgc acttcgagtc ccgcttcgct gggatcggta atctgataaa
     1681 agtacgtgac gttcgagcca tcaatgatgc gtgatgattc agcgccgacg ttgtgtttcc
     1741 aaagaagtcc caggcaccca agcccggtta ccagtgccac gaggcccgcc ctattccggc
     1801 gggagatgcc catgactcga ttccctccct gactcttccc gggtggggcg attctagtca
     1861 gaagcaacgg gccgtcattc cctcgtctgt gacgtcatgg tctgacctca tcgaggaatt
     1921 gagtcggttc ggatagaggc ttttcggaga tcttcgtgat cttggtgatt tttccggtca
     1981 aatcgacctc tatcctgacc cgatcgccct tagcgacctg ctcccgtttc aacatcgtgg
     2041 aatcggacag cgcgagtgcc caggtttgtc cttccaatgt ctggaatgtg attgtccgct
     2101 gggcttgatc cataccgacg atggcaccag caaaggtggt cgcggcttca ccggtgagga
     2161 cagccacgac cgccgtccag agcagataga tcgtcgtgag tgcgaacatc gtcctcttca
     2221 tagtgcacct ccgagccata acgatatggt
//
LOCUS       DATIPF010000294         4814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_411314, whole genome shotgun sequence.
ACCESSION   DATIPF010000294 DATIPF010000000
VERSION     DATIPF010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411314"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VLL06_07360"
     CDS             complement(<1..193)
                     /locus_tag="VLL06_07360"
                     /inference="COORDINATES: protein motif:HMM:NF015699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HSS30819.1"
                     /translation="MTGFGRRQAPWQDGSVTVEMRSVNHRFLEIACRLPRPLSHLEDS
                     FKKAIQQRCARGRIDITVTV"
     gene            complement(202..864)
                     /gene="nth"
                     /locus_tag="VLL06_07365"
     CDS             complement(202..864)
                     /gene="nth"
                     /locus_tag="VLL06_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HSS30820.1"
                     /translation="MATKSLLSSDHRKRLREIAETLERTMQHPKMELDHRSPWELLVA
                     TILSAQCTDQRVNQVTPSLFQRYRTPTEMAEADPPELEELIRSTGFFKNKAKHLVACG
                     KAVTERFSQLVPRTMEELITLPGVGRKTANVILGNAFGQPSVVVDTHVKRVAKRLGLT
                     KSDNPDLVEQDLQRLMPQSQWTAFSQRLLLHGRYVCLARKPQCRTCQVYRHCPWKEKG
                     PQ"
     gene            complement(899..2635)
                     /gene="hflX"
                     /locus_tag="VLL06_07370"
     CDS             complement(899..2635)
                     /gene="hflX"
                     /locus_tag="VLL06_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HSS30821.1"
                     /translation="MIRCTGHRTSYYKETTPIPEIYGQVLGLRAGQLAMLERLYRRRV
                     PVDQVLSLEAAKTLAQFTHEIRRPIGLVLNRRGAVQDVLVGAGTEPSPTTLAAHRASP
                     RSLRGLRLIRSQVKDQPLSQEDLTILGLLRLDMIGVLAVTPKGEPGLLSLAHLNPPTP
                     QGQLYTLLKPTLVHQCPVSFETFIHELESDLQRQSGSHMTAQGQTAILVSASPKSKPE
                     QEERLTELAELASSADLTVIDRLVQRTHSGQHRFQLGSGKLKDVLMHAMQKGADLLIF
                     DQDLPPAQVREISEITDLTVIDRTQLILDIFAQRAHSREGKVQVELAQLRYLLPRLSG
                     QGTSLSRLGGGIGSRGPGETKLETDRRRIRERIDHLENEIKGFARHQDQRRSRRVRQE
                     FPVLSIVGYTNAGKSTLLNALTKSQVSTAPRVFETLDTTSRRLHFPQGREVIITDTVG
                     FIRDLPKDLLAAFRTTLDELRDADLLLHVVDAGTKDLDAHIAAVEVVLQSLTLDQLPR
                     VLIFNKCDQLPTGQAETLCRRYDAIGISSLHQETLPPLIHHLEERLAALVPAGSSSQK
                     SAAQTFTLASRP"
     gene            complement(2632..3669)
                     /gene="tsaD"
                     /locus_tag="VLL06_07375"
     CDS             complement(2632..3669)
                     /gene="tsaD"
                     /locus_tag="VLL06_07375"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HSS30822.1"
                     /translation="MDTVERSTSVWTPGPILGIETSCDETAASVLAEDGVVLSNIITS
                     QHAIHQRFGGVVPELASRAHIESIEGVSAEALQQANLSWTDLSGIAVTQGPGLAGALL
                     VGVNYAKALAYALNIPVVGVSHLEGHIASAWLQDPTFPLPCVVLVVSGGHTHLYYKEP
                     LGHCSLLGSTRDDAAGEAFDKGAQLLGLDYPGGPAIDRLARQGSPNAIAFPRSYLNKG
                     SLDFSFSGLKTSLLYKLQAMDEQSRTTRAADLSAGYQEAIVTVLVEKAFAAVRSSGVR
                     ALAVVGGVSANSRLRALLQQRAISEDLRLSLPALAYCTDNAAMIAAAGRQALRAGLRA
                     PLDIDAQASLA"
     gene            complement(3822..>4814)
                     /locus_tag="VLL06_07380"
     CDS             complement(3822..>4814)
                     /locus_tag="VLL06_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSS30823.1"
                     /translation="KMTGIPLFKLEEEESNKLLRMEEFLHKRVVGQNEAISAVARAIR
                     RSRAGLKDARKPIGSFIFLGPTGVGKTELARTLAEFLFNSEDALIRIDMSEYQEKFTS
                     SRLFGAPPGYVGYEEGGQLTERVRRRPYSVVLFDEIEKAHPDVFNILLQVLDDGVLTD
                     SLGRKVDFKNCVIIMTSNIGTKMIQKGVSLGFQSTEGDQERHKKEEVLGELRRSFSPE
                     FLNRIDEIVVFHQLDKTHLYNILEILLGELNLRLLDKGVELEVEDDVKQWLIKEGYEP
                     LYGARPMRRTIQRAIGDPLSEEMIRGRFKECRKIRVVLRDGAPAFIEQEAMAGV"
BASE COUNT          996 a         1284 c         1431 g         1103 t
ORIGIN      
        1 ggacggtcac cgttatatcg atgcgcccgc gggcacagcg ttgctggata gcctttttga
       61 aggagtcttc caagtggctc aatggccggg gaagtcggca ggcgatttcg agaaaacgat
      121 ggttgacgga tcgcatttcc accgtcacgg acccatcctg ccagggggcc tgtcgtcgac
      181 cgaaaccggt catactacga atcattgggg acctttctct ttccatgggc aatgccggta
      241 gacctgacag gttcggcatt ggggttttct cgccaggcag acatagcggc cgtgtaacaa
      301 taaacgttgc gagaaggccg tccactggga ttgtggcatg agccgctgca ggtcttgttc
      361 gacgaggtcc ggattgtccg atttcgtcag gccgagtcgt tttgccacgc gtttcacatg
      421 ggtatcgacg acgacgctgg gctggccgaa ggcgttgccg agtatgacat ttgcggtttt
      481 tctgccgaca cctggcaagg tgatcaactc ttccatcgta cgcgggacca gttggctgaa
      541 tcgctcggtc acggctttgc cgcaggcgac gagatgtttc gctttgttct tgaagaagcc
      601 ggtggaacga atgagttctt cgagttcagg gggatcggcc tcagccattt cagtcggggt
      661 tcgataccgc tgaaagagtg atggcgtgac ctggttgact cgctggtcgg tacattgggc
      721 ggagaggatc gtagccacga gcagttccca tggggaacga tggtcaagtt ccatctttgg
      781 gtgttgcatc gtccgttcga gcgtctccgc aatctcacgc agacgcttcc gatgatccga
      841 tgaaagcagg gatttcgttg ccattgctgg taccaaaggg ggccaattgt gcagctggtt
      901 agggtcgaga tgcaagtgtg aatgtctggg ctgccgattt ctgggaagag ctgcccgctg
      961 ggacgagggc ggccaaccgt tcttctaggt ggtggattag agggggcaag gtctcctgat
     1021 ggagggacga aattccaatc gcatcgtagc gccggcatag ggtctctgct tgcccggtcg
     1081 gaagctgatc gcatttattg aatatgagga cccgtggaag ctgatcgagc gtgagacttt
     1141 gaagtacgac ctcgaccgcg gcaatatgtg cgtcgagatc tttcgttccc gcatcgacga
     1201 catgtaagag gagatcggca tcgcgcagct cgtccaatgt cgttctaaag gctgcgagga
     1261 gatctttggg caggtcgcgg ataaacccca ccgtatcagt gatgatcacc tcacggcctt
     1321 ggggaaaatg cagtctgcgg ctcgtggtgt cgagtgtttc gaagacacgt ggggccgtgc
     1381 ttacctgact tttcgtcaag gcgttgagca atgtggactt gccggcgttc gtatagccca
     1441 cgatggagag gaccgggaat tcctgtcgga cgcggcgtga tcgccgttgg tcttgatggc
     1501 gggcaaaacc cttaatctcg ttctccagat ggtcgatgcg ttctctgatg cggcggcggt
     1561 ccgtttcgag cttcgtttca ccggggcctc tactgccaat gccaccgccc aagcgtgaca
     1621 ggctcgttcc ttgaccggag agacggggga gcagatagcg gagttgcgcc aattcgacct
     1681 gtaccttgcc ttctcggcta tgcgcccgtt gcgcgaagat gtcgagaatc agctgggtcc
     1741 gatcgatcac cgtcagatcg gtgatctccg aaatctcgcg cacctgagcc gggggcaaat
     1801 cctgatcaaa aatgaggagg tctgcgccct tttgcatggc gtgcatgagc acgtctttga
     1861 gcttgccgct tcccagttga aaccggtgct gaccgctgtg cgtgcgttgc actaaacggt
     1921 cgatcaccgt gagatccgca gaggaagcca gttctgccag ctcggtgaga cgttcttctt
     1981 gctcgggctt actcttgggc gacgcgctta cgaggatggc ggtctgaccc tgggccgtca
     2041 tgtgcgatcc gctctgacgt tgaaggtcgg actccagttc gtgaatgaat gtctcgaaac
     2101 tcaccggaca ttgatgcacc agtgtcggtt tgagcaatgt atataactgc ccctggggag
     2161 tgggtggatt cagatgggcc agagagagca accccggttc gcccttgggt gtgacagcca
     2221 agacaccgat catgtcaagt cggagcaggc ccaggatggt gagatcttct tgactcaggg
     2281 gttggtcctt cacctgagag cggatcaggc gaaggcccct cagcgatcgt ggacttgcac
     2341 gatgcgcggc cagtgtagtc ggcgagggtt cagtgcctgc gccgaccaga acatcttgta
     2401 cagccccacg tctgttcagt accagtccga tgggacggcg gatctcgtgt gtgaactgcg
     2461 cgagggtttt tgcggcttca agcgagagga cttgatccac gggcacacgt cggcgataca
     2521 gtcgctcaag cattgcgagc tggccggcgc ggagaccgag gacctgtcca taaatttctg
     2581 gaatgggcgt cgtttcctta tagtagctgg tgcgatggcc tgtacaacgg atcaagccag
     2641 cgaggcttgg gcatcgatgt ctaggggtgc acggagccct gcacgcagag cttgcctccc
     2701 tgctgctgcg atcatcgcgg cgttatcggt acagtaggcc agcgcgggaa ggctgaggcg
     2761 caggtcttcg gatatggctc gttgttggag cagcgctcga agccgtgaat tggcggatac
     2821 ccctccgacg acagccaacg cacggactcc ggatgagcgt actgcggcaa aggccttctc
     2881 aaccaagaca gttacgatgg cctcttgata gccggccgag aggtcagcag ctcgcgtggt
     2941 gcgagactgc tcatccattg cctgaagttt atagagcaaa gatgtcttta gcccactaaa
     3001 actaaaatca aggctcccct tattcagata ggaacgggga aatgcaatgg cattcgggct
     3061 cccctgtctg gctaaacgat cgatcgctgg tccgccaggg taatctaatc caagtaactg
     3121 ggcgcccttg tcaaaggcct cccccgcagc atcgtctctg gttgagccga ggagactgca
     3181 gtgaccgagg ggctccttat agtacagatg ggtatgtcca cccgagacaa ccaagaccac
     3241 gcagggcaac ggaaacgtcg gatcttgcaa ccaggcggag gcgatgtggc cttcgagatg
     3301 gctcacaccg accaccggaa tatttaaggc gtaggccagt gcttttgcat agttaacccc
     3361 gacgagtaac gcgccggcca ggccaggtcc ttgggtgacg gcgattccgc tgagatccgt
     3421 ccaggagaga ttggcctgtt gcagtgcctc cgcggagacc ccttcaatcg attcaatgtg
     3481 ggcacgagag gcaagttcgg gaacgacacc gccgaatcgc tgatgaatgg catgctggga
     3541 tgtaataatg ttcgaaagga cgacgccgtc ttcggcaagc accgatgcgg cagtttcatc
     3601 acaggacgtc tcgatgccaa gaataggccc aggtgtccaa acggaggttg atcgctcaac
     3661 agtgtccatc ttgatatctc tgccgattac cctgactggg cggataacac gtgcatgaac
     3721 atggtcgaag gaccaaataa cacagccctt gtggccgttg gcgccacaag ggccgtatcg
     3781 tactctatgc gatggcgtcg cgcaggtaca gggctaggaa attacacgcc agccattgcc
     3841 tcttgctcga taaatgccgg agctccgtca cgcagcacca cgcggatctt tcggcattcc
     3901 ttgaatcgtc ctctgatcat ctcttcggag agtgggtcac cgatcgcgcg ctgaatcgtc
     3961 cggcgcatcg gtctggctcc gtagagcggt tcataacctt ctttgatcaa ccattgcttg
     4021 acgtcgtcct caacttcgag ctcgacaccc ttatcgagca gacgaaggtt aagttcaccc
     4081 aagaggatct cgagaatatt gtagagatgt gtcttgtcca attgatggaa gaccacaatc
     4141 tcgtctatcc gatttaagaa ctccgggctg aacgatcggc gtaactctcc caacacttct
     4201 tccttcttat gccgttcctg atcgccttcc gtgctctgga atccgaggga gacccccttc
     4261 tggatcatct tggtcccgat attggacgtc atgatgatga cgcaattctt gaagtcgact
     4321 ttgcgtccga ggctgtcggt aaggaccccg tcgtccaata cttgtaacag gatattgaat
     4381 acatcggggt gggccttttc gatttcatcg aacaggacca ccgaataggg ccgacgccga
     4441 acccgttcag tcaattggcc gccttcttca tagccaacgt atccaggagg ggctccgaag
     4501 agccgagaac tggtgaactt ttcctggtat tcggacatgt cgatccgaat gagtgcatct
     4561 tcactattga agagaaattc cgctagcgtt cttgccagct cggtttttcc gaccccggtc
     4621 ggtcccagga agataaagga cccgataggc ttccgtgcgt ccttcaatcc agcgcgtgac
     4681 cgccgaatgg cccgggccac agctgagatc gcttcgtttt gtccgaccac acgtttatgg
     4741 aggaactcct ccatccgaag cagtttgttc gattcttctt cttctagttt gaagagcgga
     4801 atgcccgtca tctt
//
LOCUS       DATIPF010000295         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1208224, whole genome shotgun sequence.
ACCESSION   DATIPF010000295 DATIPF010000000
VERSION     DATIPF010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208224"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..686)
                     /locus_tag="VLL06_07385"
     CDS             complement(9..686)
                     /locus_tag="VLL06_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30824.1"
                     /translation="MKSLRGVISQSALILMGGLLLVFSVLIYVGGDALLHRFIDGRLL
                     GLAETLATIVERNPDIIKNSGDDPSLAAEVIRHENQRHELHEVTHSLMVFSPEGRVVW
                     KGPEAVAQHSAPDETVFERARRGITVFETVKAADGKPIRHLFLPIPREGQVRYVLHAE
                     ASMLLYQETLKGFVVLLTLGSGVILLIAWIGSGWLAKKVLTPIDVLSTGAETMSEADL
                     GKRLTLD"
     gene            complement(683..1555)
                     /locus_tag="VLL06_07390"
     CDS             complement(683..1555)
                     /locus_tag="VLL06_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSS30825.1"
                     /translation="MIFAGQVAVERPCSQTFDDIPTDLGLSDLPVERCHNSISQDIRS
                     VDDGSSCLPVIRKVGTTEAMRILLVEDDLNLAQFIRKGLKEEQYAVDFAADGEQGLEL
                     ALLNPYDLLILDIMLPKLDGLTICRRVRAKGNATPVLLLTARNTVEDKVSGFDTGADQ
                     FLTKPFAFAELLARVRALLRRGAPLQLVHLKAADLELDPASHRVWRAGQEIALTNKEY
                     ALLEFLLRNKNRVLTRTAIIEHIWDISYDPMTNIVDAHIKALRAKVDRDFSPQLIATV
                     RGAGYMLEEPEAIA"
BASE COUNT          500 a          630 c          584 g          494 t
ORIGIN      
        1 tgatagggtc agtccagggt aagtcgtttt cccaggtcgg cctcagacat cgtctcggcg
       61 ccggtactta acacgtcgat tggggtcagc actttcttcg ccagccatcc actgccgatc
      121 caggcgatga gaaggatgac cccggaccca agagtcagga gcaccacaaa gcccttgagt
      181 gtttcttggt acaggagcat ggaggcttcc gcatgcagaa cataccgcac ctgaccttcc
      241 cgaggaatgg ggaggaacag atgccgaatg ggcttgccgt cggcagcctt gacggtttca
      301 aacaccgtga tcccgcggcg agcccgttcg aacaccgtct catcaggagc agaatgctgt
      361 gcgaccgcct caggcccttt ccagaccacc cgaccctccg gcgagaagac cataagagaa
      421 tgggtgacct catgcaattc atgccgctgg ttctcgtgtc ggatcacctc agcagcgagc
      481 gaagggtcat caccggagtt cttgataata tcgggattcc gctcaacgat cgttgccaga
      541 gtctccgcca atcctaacag ccgaccatcg ataaaacggt gcagcaacgc gtcgcctccg
      601 acgtagatca gcacagaaaa gaccaggagg agtcctccca ttaaaatgag cgcagactgg
      661 ctgatcacac cacgtaggga cttcatgcga tcgcttctgg ttcctccagc atgtaccccg
      721 ctcctcgcac tgtggcaatc agctggggtg aaaaatctcg atcaaccttc gcacgtaggg
      781 ctttaatatg ggcatccaca atgttcgtca tggggtcgta actgatgtcc catatatgtt
      841 cgatgatcgc cgtccgcgtc aatactcgat ttttattccg caggaggaac tcgagcaggg
      901 catattcttt attggtcagt gcgatttctt gacctgcccg ccagacgcgg tgagacgcag
      961 gatccagctc caggtctgcg gctttcagat gaaccaattg gagcggagct cctcgacgca
     1021 gcaaggctcg gactcgggcc aacagttccg cgaaggcgaa cggtttcgtc agaaattggt
     1081 ccgcgccggt atcgaacccg gacaccttgt cctctacggt gttgcgtgcg gtcaacagca
     1141 acaccggagt cgcgtttccc ttggcccgga cacggcggca gatcgtgagc ccatcgagct
     1201 tgggtaacat gatgtcgaga atcaaaagat cgtaaggatt cagcaaggcc aactccaatc
     1261 cttgctcacc atcggccgcg aagtccaccg catactgctc ttccttcaac cccttgcgga
     1321 tgaattgagc gaggttcaga tcgtcttcaa ccagcagtat gcgcatggcc tccgtggttc
     1381 ccaccttccg aatgaccggc aagcacgacg atccgtcatc gactgaacgg atgtcttgac
     1441 tgatactatt atggcatctc tcaaccggca ggtcactcag ccctaagtcg gtcgggatgt
     1501 cgtcgaacgt ctgggaacag ggtcgttcga cagctacttg cccagcaaat atcatcaact
     1561 catctccctc gcatcagatg ccatccgtgt attcgccgca tatatccgag agggatcatc
     1621 tcctcaaggc cgcctccatg cacaatccca caactccaac caaacagaag ataaaaaccg
     1681 ctccataata catggcatca attaaagcgg cgtgcatcat gctgtgtctc ctttctcgaa
     1741 aatccttcgg ccgtttggac cgagggggta ttcactatca gttgccccag aacggcctca
     1801 ggccccgctt cgtccaacgt cttttcgtcg tgaggctagt gtaatgattg aggatgaatt
     1861 gaggatgaag ccaacgtgaa gaacctctaa tggtttggac cggccgtttt catatgggcc
     1921 gttcgccgat cgaatgggat gtggcgaatg gacacacccc tctatggatt ctggtgttga
     1981 agaagcgttc ttacattgag catcggaatg aagcgggagc gcggtatggg aaaggtaggt
     2041 ccctgggcaa agcggccctc ggcaaccgca gggcgctagg gaagcagatt ttttagcatt
     2101 ttgctagagc cgctcgccgt acttgcgaaa gtgctctttt ttggcttcgt cgatccattt
     2161 gtcccgcttg attcgttcag gggcggtgta gagcttcttc gcgacctt
//
LOCUS       DATIPF010000296         1997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_941136, whole genome shotgun sequence.
ACCESSION   DATIPF010000296 DATIPF010000000
VERSION     DATIPF010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941136"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VLL06_07395"
     CDS             <1..290
                     /locus_tag="VLL06_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30826.1"
                     /translation="LFLSVGVLASALTENQIVAAFVSIGLLLVLWLLAGLGSLLGDTA
                     LGQAVSYLSFMEHYDHLVRGLVDTKDLVYFFSGLVLMLFLAHRVVDSARWK"
     gene            287..1810
                     /locus_tag="VLL06_07400"
     CDS             287..1810
                     /locus_tag="VLL06_07400"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HSS30827.1"
                     /translation="MNFKTIPLGAIGMVLAVAGAVGYSLAPEKLWLVTLLEGSALFCL
                     ILFVVVHFISLKAFSARRSTRMGANSLLMTLLFVGILAIINFLAASHSIRWDLSENQN
                     FSLAPQTHRVLRSLSREVLITVFTREKDPGYQAYKERLDSYRQASPKISVEFIDPERQ
                     PKIAQNYGISRTDTAVFESSGHTVRVTAPSEVELTGALIRVSQDSKKRVLFLEGHGEP
                     SLDDRERTGLSAAKEILIKQGYDVGTLSLLKEEAVPDRTAILVVAGPRRPVTSDEQER
                     IHHYVEKGGHLLLLIDPDTQADLNPLLNQWGLGVGPGALVDLQDRLAQGDLTSLLVRT
                     FTEHEITQDLSAAVLFPLARHITFDEQVGNAWDYVPLARTSPNSWAETDLKGRVVNLN
                     EREDIKGPLPMAAALTPKVAPEEGKPRPAIVVIGNSTFATNAFVNFPGNSDFFLHTAA
                     WLAEERDMMAIVPRDSALRLFTPNPLQDRALLYLQVILLPATMLFSGIMVWRKRRRL"
BASE COUNT          412 a          603 c          519 g          463 t
ORIGIN      
        1 ccctcttcct gtccgtcggc gtgttggcct ctgcactgac cgaaaaccag atcgtcgcgg
       61 cctttgtcag cattggtcta ttactggtcc tgtggctcct tgccggctta ggctcactgc
      121 tcggcgatac ggctctcgga caggctgtct cgtatctgtc atttatggag cactacgatc
      181 atttagtgcg cgggctggtc gacacaaaag acctcgtata ttttttcagc ggtttggtat
      241 tgatgctctt tttggctcac cgagtcgtgg actcggcacg gtggaaatga acttcaagac
      301 cattccactt ggcgcgattg gaatggttct ggccgtggcc ggagcggtcg gctatagcct
      361 tgctccggag aaactctggc tggtcaccct cctggaagga tcggcactct tctgcctcat
      421 actctttgtc gtcgttcatt tcatcagtct gaaggccttc tccgccaggc gctctacgcg
      481 tatgggtgca aacagtctgc tgatgaccct cctcttcgtc ggtattctcg cgatcatcaa
      541 ctttctcgcc gccagccact ccattcgttg ggatctgtcg gagaaccaga atttttccct
      601 cgctccgcag acccatcgcg tactgcggag tctttcgcgc gaggtactta tcaccgtgtt
      661 caccagggag aaagatccag gttatcaagc ctacaaagaa cgcctagata gctatcggca
      721 agccagtccg aagatttccg tggaattcat cgacccggaa cgccagccga aaatcgccca
      781 aaactatggc atttccagaa ccgatacggc cgtgttcgaa agctcaggcc atacggttcg
      841 ggttaccgct ccttctgagg tggaactgac aggggcattg atccgcgtgt ctcaggacag
      901 caagaaacgt gtgctctttc tcgaaggaca cggtgagcca agcctcgacg atcgagagcg
      961 aaccggattg tctgctgcaa aagaaatctt gatcaagcag ggctacgatg tcggtaccct
     1021 cagtttgctc aaagaggaag ccgtgccgga tcgcaccgcc atcctcgtcg tggctgggcc
     1081 ccgtcgtccg gtgacttccg acgaacaaga acgcattcac cactatgtgg aaaagggcgg
     1141 ccatctcctg ctgctcatcg atccagatac gcaggctgac ctgaatcctc tgctcaacca
     1201 gtggggcttg ggagtcggcc ccggagcctt agtcgatttg caagatcgac tagcccaagg
     1261 ggatctgacg tccctgctcg tccgtacctt cacagaacat gaaattaccc aggacctctc
     1321 cgccgccgtc ctgtttcccc ttgcgcggca tatcaccttc gatgaacagg tcggcaacgc
     1381 atgggactat gtgcccctcg ccagaacgtc accgaatagc tgggcggaaa ccgatctgaa
     1441 aggccgcgtc gtgaacctca atgagcggga agacatcaag gggcccctgc cgatggccgc
     1501 ggccctcaca ccgaaggtcg caccggagga aggcaagcct cggcctgcga ttgtggtcat
     1561 cggcaattct acatttgcga ccaatgcctt cgtgaatttt cccggcaaca gcgatttttt
     1621 tctccatacg gctgcttggc tggctgaaga acgggacatg atggcgatcg tccccaggga
     1681 ttccgcgctt cgcctgttta ctcccaaccc cttacaggac cgagcactcc tctatctcca
     1741 agtcatcctt ctgccggcca ccatgctgtt ttccggtatc atggtttggc gcaaacgccg
     1801 acgcctctag atcaatgcgt tactggccca cactcgtcat ggtgctcgta ttggctggac
     1861 tcggcctcta tctctacatg atcgaactcc cgcagaaagc gtcgcaggag cgacaagaca
     1921 ccatcgatac gagggtgctg ctcttcgacc aagatacgct ttccggactt accatgaaga
     1981 ccgaccggca cgaactc
//
LOCUS       DATIPF010000297         2601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1733382, whole genome shotgun sequence.
ACCESSION   DATIPF010000297 DATIPF010000000
VERSION     DATIPF010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733382"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VLL06_07405"
     CDS             <1..596
                     /locus_tag="VLL06_07405"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class GN sortase"
                     /protein_id="HSS30828.1"
                     /translation="LLAYSLVLVGLWQAGSGAWIYVKAQLAQVLLQRAWAGTLAGQQN
                     VKPWPWADTWPIARLVVPRLGIDQIVLEGAYGRTLAFGPAHVESRSDAREGTVILTGH
                     RDTHFAFLEHVQTGERFALQSSNGHIRSYRVGDHRIIDSRSGRIPIDREHPALVLVTC
                     YPFHTAIPGGPLRLVVTAAAEDSEMERKVRTVGTSGG"
     gene            complement(583..1074)
                     /locus_tag="VLL06_07410"
     CDS             complement(583..1074)
                     /locus_tag="VLL06_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HSS30829.1"
                     /translation="MVKRYVMTGLVLALVAACPAISWADFLARVVTIHEGDRLTIRHD
                     GRNETIYIKDIDCPELKQAYGKQAKHAISAYVGSRDVMVRALKRGRNGLGTAEILLQD
                     GRNVGHELLKEGLAWARPERGQDQSLEDMEQLAKAERKGLWSDPNPVAPWKWKPTSKV
                     IHR"
     gene            1453..>2601
                     /locus_tag="VLL06_07415"
     CDS             1453..>2601
                     /locus_tag="VLL06_07415"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HSS30830.1"
                     /translation="MITRRFASQAGAALLAAVLGVTQASAEATPPHQHTAVMNPETEA
                     AATVIHRPAGPVLQDKMARAIEQIERQVQSKGPFQGAGSHAMQQGVLLVAEDQDKVKV
                     GQGSRCPASAPVRTFDISAINVEITVNRFGDFYPGYMYALTENVPGIRTEEEKNKTAR
                     ESDDVTFSAGAVSNGLQGDLIQPLVIRANQGDCMRVTLRNQIADEPTNMIINGSQMLV
                     RSTGKPANSNNPEALVETGKVGEFEWYIQPDLQEGGHAFHSHTTREQYSLGMLGSLVV
                     EPRGARHLSPFTGEEMTSGWEAIIDLAKGSDFREFVIFYHEAGDETFRLLSRTGDMLP
                     QRDPHTDTYRPAARLLNYRSEPHGTRLEQQAHLIGFSDESQGYGSYTFG"
BASE COUNT          552 a          744 c          763 g          542 t
ORIGIN      
        1 ccctcctggc atattctttg gtcttggtcg gcctatggca ggctggtagc ggagcctgga
       61 tctatgtgaa agcccaactg gcccaagtcc tgttacaacg ggcttgggcc ggcacgttgg
      121 ccggacaaca aaacgtgaaa ccgtggccct gggccgatac ctggccgatc gctcgattgg
      181 ttgtgcctcg tctgggcatc gatcagatcg tcttagaagg cgcctatggc agaaccttgg
      241 cgtttggacc agcgcatgtg gagtcacggt ccgatgctcg tgaaggaacg gtgatcctga
      301 ccgggcatcg tgatacccac ttcgcgtttc tggaacatgt tcagactggt gagcgattcg
      361 ctctgcaatc gtcgaacggc cacatccgtt cttatcgagt cggcgatcac cggatcatcg
      421 atagcagatc aggcagaatc ccgattgacc gggaacaccc ggcactggtc ttggtgacct
      481 gttacccctt tcacacagcg attccaggtg gtccgttgcg cttggtcgtc accgcagcgg
      541 cagaagatag tgaaatggag aggaaggtaa gaacggtcgg gactagcggt ggataacctt
      601 cgacgtcggc ttccacttcc acggcgctac gggattggga tccgaccaca ggcccttgcg
      661 ttcggctttg gccagctgct ccatatcctc aaggctctgg tcctggcctc tctcaggtcg
      721 tgcccatgcc agcccttctt tgagtagttc atgccccaca ttgcgtccgt cctgaagcag
      781 aatttctgcc gtgcccaggc cgttccggcc ccgcttgaga gcccggacca taacgtcgcg
      841 gctcccgaca taggctgaga tcgcatgctt ggcttgtttc ccgtacgcct gcttgagttc
      901 gggacagtcg atatctttga tatagatcgt ttcatttcgg ccatcgtgac ggatcgtcaa
      961 gcgatctcct tcatgaatcg tgacgactct cgcgagaaaa tccgcccatg aaatcgccgg
     1021 acaggcggcc accaaggcga gtacaaggcc ggtcatgacg taacgtttca ccatggtccg
     1081 tatgctagcg cgtctcgcct atcctgccaa ctctttctcg atttttgacc acgaggcact
     1141 ccgtagaggc tgaagagtct cacaggctgc ggaaaatcta ttctcattct tttcgcccat
     1201 accgtatttg atcgagcaag tcttcctatg ttagtatcgg accgtttcag tgattgagat
     1261 gtgaagacct gcctggtgaa agctgcgcga tgaatgaacc ggcctcttga attcgcaacg
     1321 acgaagggcg gagccttcat cagaccactt tgggagacag gacgcggacg cgcatcacgg
     1381 cgcgggcgtt gagagtacag agctgctgcg gatagaatgt attcaccgga tcttatttag
     1441 gaggagacga ggatgattac cagaaggttt gcaagtcagg caggggccgc tttgctcgcc
     1501 gccgtgttgg gcgtgaccca ggcgtcggca gaagcgacgc ctcctcatca acatacagcc
     1561 gtcatgaacc ctgaaacgga ggcggcggca accgtgatcc atcgacccgc aggaccagtg
     1621 ttgcaggaca agatggcgcg ggcgatcgag cagatcgagc ggcaagtgca gagcaagggc
     1681 cccttccagg gagcaggctc gcacgcaatg cagcaaggcg tcttgctggt ggcggaggac
     1741 caggacaagg tgaaggtggg tcaggggtcc cgctgtccgg ccagcgcacc agttcgcaca
     1801 ttcgatattt ccgcgatcaa cgtcgaaatt accgtgaacc gcttcggaga tttctatccc
     1861 ggatacatgt atgccctcac agagaatgtg ccgggcatcc ggaccgagga agagaagaat
     1921 aagacggcgc gtgaaagcga cgacgtcacc ttctcggccg gtgcggtgtc caacggtctc
     1981 cagggtgatt tgattcagcc gctcgtcatt cgagcgaacc agggcgattg catgcgggtc
     2041 acactgcgca accagattgc cgacgagccg accaacatga tcatcaatgg ctcccaaatg
     2101 ctcgtgcgca gcacgggcaa gccagccaac agcaacaatc ctgaagccct cgtggagaca
     2161 ggaaaggtcg gggagtttga gtggtacatt caaccggatc tgcaggaggg cggtcatgcc
     2221 ttccacagcc atacgacgcg cgagcaatat tcgctcggga tgctcgggtc actcgtcgtg
     2281 gagccccgcg gcgcccgcca cctgagcccc ttcaccggcg aagagatgac cagcggctgg
     2341 gaagccatca tcgacctggc caaggggtcg gacttccgtg aattcgtgat tttctatcat
     2401 gaggccggcg acgaaacatt ccgtttgttg agccgcacgg gcgacatgct gcctcagcgt
     2461 gacccccata ccgatacgta tcggccagcc gcgcgcttgc ttaactaccg gagtgaaccg
     2521 catgggacca gacttgaaca gcaggcgcat ttaatcggat tctccgacga gtcccagggc
     2581 tatggatcct atacgttcgg c
//
LOCUS       DATIPF010000298         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_763345, whole genome shotgun sequence.
ACCESSION   DATIPF010000298 DATIPF010000000
VERSION     DATIPF010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763345"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VLL06_07420"
     CDS             <1..235
                     /locus_tag="VLL06_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30831.1"
                     /translation="HPQCRRADPARRGQLPEYGLCRFATKEKAESYRAYLCSPACPEP
                     LKEQDLTIEPYDLAAHEDLINNHPLHSAWESPT"
     gene            complement(307..825)
                     /locus_tag="VLL06_07425"
     CDS             complement(307..825)
                     /locus_tag="VLL06_07425"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS30832.1"
                     /translation="MGRVLSCPLCQKDGLLRSHPQSIRERLASFFFVAPFRCPSCSHR
                     FLASRLGLDHPTHPIDRREHLRIPVRLFLSFSGGKVCGEGTVLDLSMGGCIIKSKAQV
                     HTDDIFYLQLSLGEGESPLEVAAMVRSVSARGIAFQFLRAAHGDKRLLAFVQAHTPAQ
                     PDKSSLNAGVAA"
     gene            complement(963..2258)
                     /locus_tag="VLL06_07430"
     CDS             complement(963..2258)
                     /locus_tag="VLL06_07430"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSS30833.1"
                     /translation="MSPHDDKYDVVVIGAGAAGLAASISAAETAAKGDRPQRIVLLDG
                     AKAIGAKILVSGGGRCNVTHDVVTPADFFGNRHILKNVLAAFSIQATIDSFASLGVEL
                     KCEETGKLFPMSDKARTVLTALADRCHELGLAIRLDHRVTDIEGPSESGSGFVLHHTH
                     GTLHTRKVILATGGRSIPKSGSDGVGYGLARRLGHRVTATAPALVPLVLEDMMFHKTL
                     SGLSLDVELTTVVNGRTVDCRTGSLLWTHFGMSGPVVMDASRFWTLAHEQGELGEMYG
                     NFLPDQTQEQVRSWFLEQVRDSPRRSVGKTLSQALPQRFSQTVIRHIGCDAHTTICQL
                     PRKDRDRLIEAFTKFRFPIVQNRGWNYAEVTAGGVSLEEISFRTMESKLVPGLYLIGE
                     ILDCDGRIGGFNFQWAWATGFLAGRAVSGCVRVDGEACL"
BASE COUNT          584 a          707 c          658 g          505 t
ORIGIN      
        1 tcacccgcaa tgccgacggg cagacccagc acgtcgtggc cagctaccgg aatatgggtt
       61 gtgccgattc gccacgaagg agaaggctga atcgtatcgc gcctacctct gctctcctgc
      121 ctgccctgaa ccgctcaagg aacaagacct caccattgaa ccttatgatc ttgccgcgca
      181 cgaggatcta atcaacaatc atccgcttca ctctgcctgg gaaagtccga cctaacggat
      241 tctaagacag ttggccagcg ctcacgcttt atggtgtgcg gtgaggttcg agaagcaatc
      301 gcctgctcag gcggcgacac cggcgttgag ggaagattta tccggctgag ccggtgtgtg
      361 ggcttggaca aaggcaagta agcgcttgtc tccatgggcg gcccgaagga actggaacgc
      421 aatgccgcga gcgctcacgg aacggaccat cgcagccacc tcaagcggag attcaccttc
      481 gccgagcgac aattgcagat aaaaaatgtc gtccgtatgg acctgagcct tgctcttgat
      541 gatacagccg cccatcgaga gatcgagcac cgttccctca ccgcaaacct tacctcctga
      601 aaacgagaga aagagtcgca cgggaatgcg aaggtgttcg cgtcggtcta tcggatgcgt
      661 gggatggtcc aatccaagac gggatgccag aaagcgatga ctgcacgatg ggcaacggaa
      721 tggcgccacg aagaagaagg aagcgaggcg ctcccgtatc gattgcggat gagaccgcaa
      781 aaggccatcc ttctggcaca gcggacaact taggacgcgg cccatatcat ctattccttc
      841 ttattcccat tgctcggacc cttcgaaaga ccaaggctct agtccacgga taaatgaacc
      901 tctcatcgct gtcaagaaaa caacggttcg gtgcgaagcc cgcattgaga gaaagagcat
      961 gttcatagac acgcctcgcc gtctactcgg acacatccgg aaaccgcccg gccagccaaa
     1021 aatccagttg cccacgccca ttggaagttg aatccgccga tccgaccatc gcagtcgagg
     1081 atttccccaa tcaggtagag gcctggcaca agcttggatt ccatcgtcct gaaactgatt
     1141 tcttcgagag agactcctcc cgctgtcact tctgcgtagt tccagccgcg attctgtaca
     1201 atgggaaatc ggaacttcgt gaatgcctca atgagacgat ctcgatcctt tctcggcagt
     1261 tggcagatcg tcgtatgagc atcgcagccg atatgtcgga tgacggtttg cgagaatcgc
     1321 tgaggaagtg cttgcgacag cgtcttccct actgagcgtc tgggactatc tcgcacttgc
     1381 tccaggaacc aggaccgcac ctgttcctgc gtttgatcgg gcaggaaatt cccgtacatt
     1441 tctcccaact ctccttgctc atgcgccaat gtccagaatc ggctcgcgtc catgaccacc
     1501 ggcccgctca tgccaaagtg tgtccaaagt aaactccccg tcctgcagtc gacagtgcga
     1561 ccattcacca ccgtggtcaa ctcgacgtca agtgagagac cggagagtgt cttatgaaac
     1621 atcatgtctt cgagcaccaa tggcacgagt gccggcgcgg tggcagtcac gcgatggccg
     1681 agccgacggg caagaccgta cccgaccccg tcacttcccg acttaggaat cgatcggcct
     1741 ccggtcgcca gaatgacctt ccttgtgtgc agggttccat gagtgtggtg gagaacaaat
     1801 ccagatcctg attcgctggg accctcaatg tctgtcacgc gatgatccag gcgaatagcg
     1861 aggcccagtt catgacagcg atcggcgagt gccgttagga ctgttcgcgc cttgtcgctc
     1921 atcggaaaga gcttaccggt ctcttcgcat ttaagttcca ctcccaacga ggcaaacgag
     1981 tcaatcgtgg cttgaattga aaacgcggcc agcacgttct taaggatgtg gcgattgcca
     2041 aagaagtctg ccggagttac gacgtcgtgc gtgacattgc aacgtccgcc gccggagacg
     2101 aggatcttgg ccccaatagc cttggcacca tcgaggagca cgatcctctg cgggcgatcg
     2161 ccttttgcgg ccgtttctgc agcggaaata gaggccgcca gcccagcggc acccgcaccg
     2221 atcaccacaa cgtcatattt gtcatcatgt ggcgacatta gagagtttta actgccagtt
     2281 aacaccccat taatcttgca atgctagagt tccccacatg caaggatgac tcgtcgatgc
     2341 tgaaatacat aagagcagga ggtgagttcg atgacctgtc aaaaatgcaa aggactgatg
     2401 gtgaaagagt ggcgacctga attttcacag gaagccgcgg tgctccgctg tatc
//
LOCUS       DATIPF010000299         3211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1119361, whole genome shotgun sequence.
ACCESSION   DATIPF010000299 DATIPF010000000
VERSION     DATIPF010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119361"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="VLL06_07435"
     CDS             <1..1193
                     /locus_tag="VLL06_07435"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30834.1"
                     /translation="NNLLPLLTQYRDAPLIKPQATLRKELDRAVARAKTDEGRKEALN
                     RFKDNELFRIDMKHIVDASPSLTDFSLALTQLAEVIMARSVVDCQTKLGRLYGRPLLR
                     NRKPCPFAVLGLGKFGGRELGYASDIELLFVYGGEGSTNGKTKLENSEYFERLVQELL
                     QWIEAKQEGIFHLDVRLRPHGGKSSLANVFEAGRDYCSPTGYAAPFERQALIKLRHIV
                     GDVSLGRQMEAHRDSFVYGTDPWDIPGAVELRRQQLRQMVKPGQMNLKYSAGGLIDIE
                     YAVQYLQVIHGHDHPSLRTPNTLQALAALVKEGLVSRTDGEMLRKAYIFTRILIDGLR
                     MVRGNAKDLVLPPPDSEEFVSLARRVGYTTDDWRACARHLQADIAHHMQATGEFFTQH
                     FGIL"
     gene            complement(1344..2753)
                     /gene="glnA"
                     /locus_tag="VLL06_07440"
     CDS             complement(1344..2753)
                     /gene="glnA"
                     /locus_tag="VLL06_07440"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HSS30835.1"
                     /translation="MNPREVLEFAKKNKVQVVDLKFVDLLGTWQHFSIPVDELSEGLF
                     KDGSGLDGSSIRGWKAINDSDLLMVPDSTTACLDPFCAVPTLSIVGNIFDPITREPYD
                     RDPRFVAQKAEKYLQSTKIGDISYWGPEAEFFIFDQVRYDQTGHSGFYFIDSEEGAWN
                     IGQDGHNLGGKIRHKEGYFPVPPTDTQQDIRTEMVLEMQKAGIYIEKHHHETATAGQA
                     EIDMRFDSLVKTADKLMMYKYIVKNVARRHGKTVTFMPKPIFGDNGTGMHTHQSIWKD
                     GKPLFAGKEYAGISQLCLYYIGGILKHAPALAAFTNPTTNSYKRLTPGFEAPVLLAYS
                     SRNRSAGVRIPMYSPSPKAKRIEVRFPDPTANPYLAFPAMLMAGLDGIENKINPGEPA
                     EKDLYDLEPEEAAKIRTLPGSLDEALRNLEQDHQFLLKGGVFSKDLIESWIRYKRTRE
                     VDAMRLRPHPYEYVLYYDV"
     gene            complement(2786..>3211)
                     /locus_tag="VLL06_07445"
     CDS             complement(2786..>3211)
                     /locus_tag="VLL06_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30836.1"
                     /translation="AVERLMSRNTRLSSDRQLPATREATEVQIDNETCDRSTIIDVFA
                     DDTQGLLYAITHSIFQLGLSVEAARISTRLDDDIQQSLRTGGRHQVADVFYVTDRRGT
                     KIEDPARLETIRSTVKEDIDRFMDRQVGASERVATGSPS"
BASE COUNT          698 a          923 c          867 g          723 t
ORIGIN      
        1 ataacaatct ccttccactg cttacgcaat accgggatgc acccctcatc aagccccagg
       61 cgaccctccg gaaggagctc gaccgtgcag tcgctcgcgc caaaacagat gaagggcgaa
      121 aggaggcctt gaatcggttt aaagataacg agctgttccg aatcgacatg aaacatatcg
      181 tcgatgcgtc acccagcttg accgattttt ccctggccct cacgcaactc gccgaggtca
      241 ttatggcccg cagcgtcgtc gattgccaga cgaagctggg ccggctctat ggccgcccgc
      301 tccttcgaaa ccgaaaaccc tgcccctttg cggtcctagg cttgggcaaa ttcggcggac
      361 gggaactcgg ctatgcgtcg gacatcgaac tcttgttcgt ctatggtggg gaaggctcca
      421 cgaatggcaa gacgaagctg gagaactcgg aatacttcga gcggctcgtt caagaactgc
      481 ttcagtggat cgaagcgaaa caggaaggca tctttcatct cgacgtacga ctacgtccgc
      541 acggaggtaa gagttcgctg gccaatgtat ttgaggcagg ccgtgactac tgtagtccga
      601 ccggctacgc cgcgccattc gaacggcaag ccctcatcaa actccggcat atagtcggcg
      661 atgtgtcgct gggccgacag atggaggcac accgggactc cttcgtgtat ggaacagatc
      721 cctgggatat tccaggggcg gtggagctcc gaaggcaaca gctcagacaa atggtcaaac
      781 cgggacagat gaatctcaag tatagcgcgg gagggctcat cgacattgag tatgccgtgc
      841 agtacctgca agtgatacat ggtcacgacc atccaagctt gcgtacgccg aatactttgc
      901 aggcgctcgc cgcactggtg aaagagggct tggtttcccg taccgacgga gaaatgcttc
      961 gcaaagccta catcttcacc cgtatcctga ttgacggctt acgcatggtt cggggaaacg
     1021 ccaaggatct tgtcctcccg ccgccagatt cggaagagtt tgtcagtctg gcccgccgcg
     1081 tcggctacac gacggacgat tggcgggctt gcgcgcgcca tttgcaggca gatatcgcgc
     1141 accacatgca agcaaccggt gagttcttca cacaacactt cggcatcctc tagcagattg
     1201 cggggaaacc cgtcacaaca aaagagcgtc cgtctcccag tcacgagaaa cggacgctct
     1261 tatctttctg tgctttctct cgaatgcctt atcgagatgt gattcttgcg tgaaccagca
     1321 tctcacatgg ctggctggtt gccctagaca tcgtaataca agacatactc gtatggatgc
     1381 ggccgtaacc gcatggcatc gacttccctg gtgcgcttat agcgtatcca ggactcgatc
     1441 aggtccttac tgaagactcc gcccttaagc agaaactgat ggtcttgctc gaggttgcgc
     1501 aatgcttcgt caagactgcc tggcagcgta cggatttttg ccgcctcctc cggctcgaga
     1561 tcgtacagat ccttttcagc cggttcacca ggattgatct tattctcgat cccgtccaac
     1621 cctgccatga gcatagcggg aaaggcgaga tagggattcg cggtcgggtc aggaaagcgc
     1681 acctcgatcc gcttggcttt ggggctcggt gagtacatgg gaatcctgac gcccgccgat
     1741 cgattccggc tggaataggc cagcaggacc ggcgcttcga atccgggggt cagccgcttg
     1801 taggagttcg tcgtcggatt ggtaaatgcg gccagggccg gcgcatgctt caggatacct
     1861 ccaatgtaat agagacagag ctgcgagatg ccggcatatt ccttcccggc aaacagtggc
     1921 ttcccatcct tccagatgct ctggtgcgta tgcatgccgg tgccattgtc gccaaagatc
     1981 ggcttaggca tgaaggtcac ggttttccca tgtcggcggg cgacgttctt gacgatgtac
     2041 ttgtacatca tcagcttgtc tgccgtctta accagcgagt cgaagcgcat gtcaatttct
     2101 gcctgaccag ccgtcgccgt ctcgtgatgg tgcttctcaa tgtagatacc ggccttttgc
     2161 atctcgagga ccatctcggt acggatatct tgttgtgtat cggttggtgg gaccggaaag
     2221 tagccttcct tgtgacggat ctttccgccc aagttatggc catcctgtcc gatattccag
     2281 gcgccttctt cggaatcgat gaagtaaaag ccgctatggc ccgtctgatc gtaccggacc
     2341 tgatcgaaaa tgaagaactc cgcctccggc ccccagtatg agatatcgcc gatcttcgtg
     2401 ctctggagat atttctccgc cttctgcgcc acgaaccgcg gatcacgatc atagggctcg
     2461 cgcgtaatcg gatcgaagat gttgccgacg atgctcagcg tcggaaccgc acagaacgga
     2521 tcgagacagg ctgtggtcga atcgggaacc attaacaggt cgctgtcatt gatcgccttc
     2581 cagccccgga tcgacgaacc atctaacccc gaaccgtcct tgaacagccc ttcactcaac
     2641 tcgtccacag gaatcgagaa gtgctgccag gtgccgagca gatcgacaaa cttcaagtcc
     2701 acaacctgga ctttgttctt cttcgcaaac tccaatacct cgcggggatt cattccaacc
     2761 actcctcctt ctcatgctcg atatattatg acggggatcc tgtcgcgacg cgttccgatg
     2821 caccaacctg tcggtccatg aaccgatcga tgtcttcctt cacagtcgaa cggatggtct
     2881 cgagacgagc ggggtcttcg attttggttc ctcgcctatc cgtcacatag aacacgtccg
     2941 ccacttggtg tctgccaccg gtacgcaagg actgttgtat gtcgtcatcc agtctggtcg
     3001 agatccgagc ggcttcgacc gacaaaccga gttgaaagat actgtgggta atggcataca
     3061 aaagtccttg cgtatcatcg gcaaacacgt cgatgatcgt ggatctgtca caggtctcgt
     3121 tatcgatctg tacctcggtc gcctcgcgag tggcaggcag ctggcgatcg gaggacagac
     3181 gggtatttcg gctcatcagc cgttcgaccg c
//
LOCUS       DATIPF010000300         4299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1288422, whole genome shotgun sequence.
ACCESSION   DATIPF010000300 DATIPF010000000
VERSION     DATIPF010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288422"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..395
                     /locus_tag="VLL06_07450"
     CDS             168..395
                     /locus_tag="VLL06_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30837.1"
                     /translation="MSLSFHGAVRSVTVSRPMMIETPGFRLLLDCGLFQGPRREEVPH
                     RDCELDFDQKLIGTMLLSHAHVPHVGSSYDV"
     gene            388..1590
                     /locus_tag="VLL06_07455"
     CDS             388..1590
                     /locus_tag="VLL06_07455"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HSS30838.1"
                     /translation="MSNGTVRPTITLLILAIGICTTSCVSAQASESEDSRSRARSLGI
                     VVGLYVPGPLNAITDVAGVTVGQTTRISGDGPLKPGFGPVRTGVTVVIPRDDVWHKKV
                     PAGSFVLNGTGEMTGLAWISESGFLEYPIALTNTLNVPRVANGVMNWMIARYPEIGIT
                     DDTLTPVVAECDDGRLNDIQGRHVSEADVIQALDSASSGAVQEGTVGAGTGMVSYGFK
                     GGIGTSSRRLPEAEGGYTIGVLVNANHGRRPELVIGGVPVGRLYETPSPVAQGISPGQ
                     SEGSIIVLIATDAPLDSRQLTRLAKRAALGLARTGSTARHGSGDFMLAFSTANIIPHY
                     PKEPTFQHLHLADTHLNPLIMATVEATEEAILNALTMATTVVGRDGHRIEAISLTRLR
                     MVLNHYTK"
     gene            1693..1920
                     /locus_tag="VLL06_07460"
     CDS             1693..1920
                     /locus_tag="VLL06_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30839.1"
                     /translation="MGGYQLGGGLSLITVLGKTNAFAEFLESRMVHALETQDPAELHY
                     LLAQLDDYHSYMWRYYNKLAKDRPERMDPGV"
     gene            1955..3178
                     /locus_tag="VLL06_07465"
     CDS             1955..3178
                     /locus_tag="VLL06_07465"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HSS30840.1"
                     /translation="MTTQSTSTTTPGTLQFATALSRKTDTEAATRDLADAIRMQIGTT
                     PIDLAFVFFSTHHATKIAMISAMLQKELRAKVCLGCSGEGVIAGSEEIETSAALTVWV
                     ACLPEVTVTPLQISVSHLQDQIRMPGWPEPGLEDSIFLLLADPFSTPMQEVLSLMTDR
                     YPNGIVGGGLAGGGQDSGENRLLLNDQVFDEGVVGVQLTGPIAVRTVTSQGCRPIGER
                     FVVTRAEQNLIFELGGVSALKRLQDVFESLGGPQRRDAHRALHLGVVIDEHGSHFERG
                     DFLVRNLIGADQQAGAVAIGDVVQEGQTVQFHLRDAKSASDDLHILLAADRTTHRNPP
                     LGALLFSCCGRGEGLFGRPHHDSGVVQERLGPIPTAGFFAQGEIGPVGGRNFLHGYTA
                     SVVIFSEPDSRKECQ"
     gene            3314..3682
                     /locus_tag="VLL06_07470"
     CDS             3314..3682
                     /locus_tag="VLL06_07470"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSS30841.1"
                     /translation="MPFQEAGAEGKEVTTTTGLQYVDLAIGTGATAEAGQTVTVHYTG
                     WLENGTKFDSSVDRGQPFSFPLGAGRVIKGWDEGVKGMKVGGKRKLTIPAKLGYGAQG
                     AGGVIPPHATLIFDVELLGL"
     gene            3691..>4299
                     /locus_tag="VLL06_07475"
     CDS             3691..>4299
                     /locus_tag="VLL06_07475"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30842.1"
                     /translation="MQRTPLIAHHQTCGAKLVDFTGWEMPIQYSGVLDEYHTVRSHVG
                     LFDVSHMGRVRIAGSGAVPFLQRVTTNDVGRLAVSQAQYSMVCNENGGIKDDIFVYRL
                     ASDEFLLCVNASNREKILTWLQSQLTQDKTVQLEDRSVELAQVAVQGPKSRELLMSLG
                     GIALEELKLHHTCNGMIGGLSCLLARTGYTGELGYEIYIDADK"
BASE COUNT          907 a         1215 c         1240 g          937 t
ORIGIN      
        1 gtgatggtcg tgctggtcgg acgggacttt tgggaccgcc tgatcaactg gcaatggctc
       61 gtcgagaatg ggttcattgc tcagcaggac ctccgcctct ttcactatgc cgaaacggct
      121 caagaagcct gggatttgat cgcgcgccat aatggagtgt cctcatcatg agcctctcct
      181 tccacggtgc agtacggtcc gtcaccgtaa gtcgccctat gatgattgaa acaccaggat
      241 ttcgactgct gctggattgc gggctctttc aaggaccacg tcgagaagaa gtgcctcatc
      301 gggattgcga attggacttc gatcaaaaac tgatcggaac catgttgcta tcccatgccc
      361 atgttccaca tgtcggatcg agctacgatg tctaacggca ctgtccggcc gacaatcacg
      421 ctgctgatcc tggccattgg catctgcaca acctcgtgcg tgtccgccca agccagtgag
      481 tcggaagatt cccgatcgcg agctcgttct cttggcatcg ttgtcggcct ctatgtccct
      541 gggccgctga acgcgatcac tgacgtggct ggcgtcaccg tgggccagac cacccgtatc
      601 tccggagacg gaccgcttaa gcctggattt ggtcccgtac gaaccggtgt gacagtcgtc
      661 atccctcgcg acgatgtctg gcacaagaaa gtaccggccg ggtcgttcgt cctcaatgga
      721 accggcgaga tgacggggct tgcctggatc agcgaatcgg gcttcctcga gtatccgatc
      781 gctctgacca atacgttgaa tgtgccacga gtggccaacg gggtgatgaa ttggatgatc
      841 gcgcgctatc cggagatcgg tattaccgac gatactctga cgcccgtggt cgccgaatgc
      901 gatgatgggc gactcaatga cattcaaggg cggcatgtct ccgaggcgga tgttatacag
      961 gcactcgaca gcgcctcctc cggagccgtg caggaaggaa cagtcggtgc tggaacaggc
     1021 atggtgtcct atggattcaa aggtggtatc ggaacctcgt cacggcgatt gccagaggcc
     1081 gaaggggggt atacgatagg cgtgctcgtc aatgcgaacc acgggcgacg gcccgagctc
     1141 gtcattggcg gagtgccggt cggtcggctc tatgagactc cgtcacccgt cgcgcagggt
     1201 atttcaccag gccaaagtga gggttccatc attgttctca tcgccaccga tgctccgctc
     1261 gatagccgcc agctgactcg attggcgaaa cgtgccgcgc tgggactggc gcgcacagga
     1321 tccaccgcgc gccacggcag tggcgatttc atgctggctt tttccacggc taacatcatt
     1381 ccccattatc ccaaagagcc gacctttcag catctgcact tggccgacac acacctgaac
     1441 ccattgatta tggccacggt tgaagccacg gaagaagcga ttctcaacgc actgaccatg
     1501 gccacgacag tcgtgggccg tgacggtcat cgcattgaag ctatctccct taccagactc
     1561 cgtatggtcc tcaatcacta caccaagtaa cggaggagga tcaggatgcc gtgagacgaa
     1621 tattctctca cttgcaattc cctctcttgc ctcgctatcc tctcgcgact ctcttttgtc
     1681 tggaggatta atatgggtgg gtatcaactc ggtggtggtt tgagtctgat aaccgtgctg
     1741 gggaaaacga atgcgttcgc cgagttttta gaatcccgca tggttcatgc cttggagaca
     1801 caagatcccg cagaactgca ctatctcctg gcacaactcg acgactacca ttcttacatg
     1861 tggcgctact ataacaagct ggcgaaggac cgcccggaac ggatggaccc aggggtctaa
     1921 cgtatcggcg accgattggc gataggtgca tgctatgacg actcaatcca caagcacgac
     1981 cactccgggc acgcttcagt ttgcgaccgc tctctctcgc aagaccgata cggaagccgc
     2041 cacgcgcgac ctcgcagacg cgatacggat gcaaatcggc acaaccccca tcgatctggc
     2101 attcgtcttt ttctctaccc accatgcaac caaaatcgcc atgatctcag ccatgcttca
     2161 gaaggaactg cgggcgaagg tctgtctggg ttgttccgga gaaggtgtga tcgccggctc
     2221 ggaagaaatt gaaacctctg cggccctcac tgtctgggtc gcatgccttc ctgaggtcac
     2281 agtgactccg cttcagattt ccgtgtctca cctgcaggat caaatccgca tgccagggtg
     2341 gccggagccg gggctcgagg attcaatctt tcttctcctt gctgatccct tttcgactcc
     2401 gatgcaagaa gtcttgtctc tcatgaccga ccgatatccc aacggcatag ttggcggggg
     2461 ccttgccggt gggggccagg attcaggaga aaatcgactc cttctcaatg accaggtctt
     2521 tgacgaaggc gtagtggggg tgcaactcac gggaccgatc gctgtccgga ccgtgacgtc
     2581 gcaaggctgc cgtccgatcg gcgaacggtt tgtcgtcacc agggccgagc agaacctcat
     2641 ttttgagctc ggcggcgtct cggccttgaa acgcctccag gacgtctttg aatcgctggg
     2701 gggaccccaa cgtcgcgatg cgcaccgcgc gttgcatctg ggagtcgtga tcgatgagca
     2761 cggcagtcat ttcgagcggg gtgacttcct ggttcggaac ctcatcggag cggatcagca
     2821 ggctggagcc gtcgcgatcg gcgatgtcgt gcaggaaggt cagaccgtgc agttccacct
     2881 tcgtgatgca aagtcggcaa gcgacgactt acacattctc ttggctgcgg atcgaacgac
     2941 acacagaaat cccccgctgg gagcccttct attcagctgt tgcgggcgtg gggagggact
     3001 ctttggccga ccccatcacg atagcggggt cgtgcaagag cggctcggcc ctatccccac
     3061 ggcaggattt tttgcgcaag gtgagattgg tccagtcgga gggcgcaatt tcctgcacgg
     3121 ctacaccgcg agtgtggtca ttttttccga gcctgactct cgcaaggaat gtcagtgaga
     3181 gccgcacgtg ttgggagacc ccaaacaggg tgtcgaccct gacgtcggag aggtttccat
     3241 acacacgaat gacggtattc tattgtcatc ggtcaccgtg gttgagtgat tgacgattag
     3301 gaaaggaacc atcatgccgt ttcaagaagc aggtgctgaa gggaaagaag tcacgacgac
     3361 gacgggattg cagtacgtcg atcttgcgat cggcaccggc gcgacggcag aggcggggca
     3421 gaccgtcacc gtgcattaca caggctggct tgaaaatggg accaagttcg acagttccgt
     3481 ggaccggggc cagccgttct cttttcctct tggagctggg cgagtcatca aaggatggga
     3541 cgagggtgtc aaaggcatga aagtgggtgg aaagcgaaag ctgaccattc cggccaagct
     3601 cgggtatggt gcacagggtg ccggcggcgt gattcctccc catgcgacgt taattttcga
     3661 cgtcgagctg cttggtctct aatccacgtc atgcaacgca cgccactcat tgcacaccat
     3721 cagacctgtg gcgcaaaact tgtcgacttc accggatggg agatgcccat ccaatacagc
     3781 ggtgtgctgg acgaatacca cacagtccgt tcccatgtcg ggctctttga tgtgagccat
     3841 atgggacggg tgcgaatcgc cgggtcaggt gcagtgccgt tcctccagcg agtcaccacc
     3901 aatgatgtcg gcagactcgc cgtttctcaa gcccagtatt ccatggtctg caacgagaat
     3961 ggtggtatca aagatgacat ctttgtctac cggctcgcct ccgatgagtt cctcttatgc
     4021 gtgaatgcct caaaccgcga gaagatcctg acgtggctgc aatcccagct tacccaggac
     4081 aagacagttc aactcgaaga ccgatcggtc gaattggccc aggtagccgt tcaaggtccc
     4141 aaatctcgcg agctgctcat gagtcttggt gggatagcgc tcgaagagct caagctccat
     4201 cacacgtgta atggcatgat cggcggcctg tcgtgcttgc tggctcggac cggttacact
     4261 ggtgaactgg gctatgagat ttacatcgac gcagataag
//
LOCUS       DATIPF010000301         6906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1029869, whole genome shotgun sequence.
ACCESSION   DATIPF010000301 DATIPF010000000
VERSION     DATIPF010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029869"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VLL06_07480"
     CDS             complement(<1..320)
                     /locus_tag="VLL06_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30843.1"
                     /translation="MMWTRKIGATMLISLLSVVWIPSGLAQANEALLVKPEPSSAPAP
                     TKPRGAASEEKPTGKRHTETSHKLASKPQPPAQVMGKDGAPMVLIPAGEFTMGSDKGD
                     DDEEP"
     gene            complement(622..971)
                     /gene="ssrA"
                     /locus_tag="VLL06_07485"
     tmRNA           complement(622..971)
                     /gene="ssrA"
                     /locus_tag="VLL06_07485"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1129..1401)
                     /locus_tag="VLL06_07490"
     CDS             complement(1129..1401)
                     /locus_tag="VLL06_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HSS30844.1"
                     /translation="MTKEELIAKMAASAGITKVAAGTALQAFTGAVTTSLKKGQRVSL
                     VNFGTFTVAKRKARLGRNPRTGEALRIPAAKVPKFSAGKILKSAVR"
     gene            complement(1504..2097)
                     /locus_tag="VLL06_07495"
     CDS             complement(1504..2097)
                     /locus_tag="VLL06_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSS30845.1"
                     /translation="MSPTSSSAFSILLLSSDQDIQSQFKHAFKDASVTVAKDVSALPK
                     DVTRRTFDAVIVELKPGSSDRATAIPLDIDLAHTMVITGSRTVLKRASKFMHMMARQN
                     AARTNGKDTLSLENFLEWKMGDFVKGMRNGSGRNLHPMLIAAIERPLITRALQETKGN
                     QIQAAELLGLNRNTLRKKIHDLHILVKRNRTTQIRGA"
     gene            2505..2954
                     /locus_tag="VLL06_07500"
     CDS             2505..2954
                     /locus_tag="VLL06_07500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSS30846.1"
                     /translation="MKVSLRATYGIMAAVDLAMQLGTAPVQAKSIARRQAIPARFLEQ
                     VLHSMKKAGLVSSLRGAQGGYVLSKKPSEVSIAEILEALDGPLLPPLGGAGRAQVRRL
                     GKPELLLGKVWEQVYQAERQVLEAISVEKLAGQQRVLEQERSLMYHI"
     gene            2963..5458
                     /locus_tag="VLL06_07505"
     CDS             2963..5458
                     /locus_tag="VLL06_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HSS30847.1"
                     /translation="MNHTPLSPVSIKTEPIPPAILEEIETFEAEARRMVSGDVSGDLF
                     RPFRLQYGIYGQRQAGVQMVRIKIPFGGLTANQLRRIAELAEHYATGVGHVTTRQDIQ
                     LHFVELTDVPTIMRGLAEVGVTTREACANTVRNVTACHLAGICQGEVFDVTPYAKTVA
                     LHLLRNPLNQSLPRKFKIAFSGCKQDCALTPIHDIGLLAAKRADGAIGFRMVAGGGLG
                     SAPRIAQVLREFTPMDELIPSIEAVIRVFDTLGNRKNRTKARMKFVIDKLGFEEFKRR
                     WEEAYVSMGHARPTHEPIKLLSHPDDPVPLIMPTSNGSKQAGIQTNGHAQSPREETPF
                     EMWRRTNVVRQKQDGYVAAVVKLFMGDLTAEQMLFIADLADHYSNGNIRTTINQNMII
                     RWVPESRIADLHADLTSQGLADPGAELVEDIIACPGTDTCGLGITSSKGLARALAEVF
                     PAGRVPEDLKDVSVKISGCHNSCAQHHIATIGLHGVGKRIGEHVAPFYELHLGGRVNG
                     TAKIGQMVVKLPAKSVSAAITHLIDVYRRDRLSGEGLPAFIDRVGKVKLKDELIPYTI
                     VPSFQDDSMFYYDWEGEEEFVLEDLGPGECAGGALEMIENGILEADQELYQAKLLVEK
                     HQYSVSVNKSYRAVLAAAKALLVTEGLEPSTDAETFVEFDSRLAQKGIVPAMYRDLRM
                     RVGDLGPKETTGESARDKMEFAKGFVDACRAATEQMGKDLKLAPVGKAAVAETSTTPE
                     VKPVVSSATTPAASGVPVYDLRGVACPMNYVKTKLKLEMMDAGEHLEVWLDAGEPIKN
                     VPLSLKNDGHKLLLQEPLEAAASHYRILVEKVE"
     gene            5764..6471
                     /locus_tag="VLL06_07510"
     CDS             5764..6471
                     /locus_tag="VLL06_07510"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HSS30848.1"
                     /translation="MESLSSKELAAWGESFEEKSPQELLAAVIERYATKIVLACSFGA
                     EDVVLVDMVHRINPSVPLFYLDTDFLFPETYATRDRVIERYGLQPQQVIQVKSLLTPE
                     QQAVQHGDALWTRHPDQCCELRKVEPLIRVLKGFDAWITGIRRDQSPTRANAKLIEWD
                     QKFQLVKVNPLAKWTWEDVWTHIRVHEVPYNELHNRNYPSIGCTHCTSPVMPGEDSRA
                     GRWKNFSKTECGLHKAS"
     gene            6447..>6906
                     /locus_tag="VLL06_07515"
     CDS             6447..>6906
                     /locus_tag="VLL06_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30849.1"
                     /translation="MWITQGLVMSMQEFIAKIGKGPKTAKDLTWDEAKRAMKVLIEGE
                     ATPAQVGGFLMAMRIKMESVTELASMTATARSYVAPVPIPRELGVVDLPSYAGKQETF
                     HALAAAAIVAASAGASVLMHGYDGIPGRPGNAGALKTLGLPIDLEPKAVAE"
BASE COUNT         1524 a         1779 c         2042 g         1561 t
ORIGIN      
        1 ggctcctcgt cgtcatcgcc cttgtcgctg cccatcgtga actcgccggc cgggatcaag
       61 accatgggcg cgccatcttt tcccatcacc tgggcaggag gttgtggttt gctggcgagc
      121 ttgtgtgagg tttctgtgtg cctcttccct gtgggtttct cttcactcgc ggctccgcgg
      181 ggtttggttg gtgccggagc cgagcttggt tcaggtttaa cgaggagcgc ctcattggct
      241 tgtgcaagac cactaggaat ccagaccacc gataacaggc tgatgagcat cgtagcccct
      301 atttttcgag tccacatcat cgtgagagat tctccggtac cgtcaatggt agggtgattc
      361 gatcactcgt ttgattccgt tcagggtacc caaaatcgcc aggacaaagc tagcatcggg
      421 tgtggccgtc agggccatgc atgactgtcc ccatggaagg gtgggcttgg cctgcagcct
      481 aggacgttta aagcctgtta ggaatcggga tattgatacg ggaagaactt gtgttgtgac
      541 gtctctcggc tgccgcagtg ccagtgagat tgtcttaccg aaagaagcgg gcccctcggt
      601 aaggtgaccg aggggcgggt ttggtggagg cgccgggagt tgaacccggg tccgaagacc
      661 ttcagtgcaa cggatctaca tgtgtagccg atcgttttag tttcgcagcc acccacgccc
      721 atcggctggc ttagactggc tgctagccca gtattgtctc gccctcagcc gctgagcacc
      781 agctttggac cagcccgctg tatcgcgctc cgaccaccgc cgcgggcctc aggtggagga
      841 acgtcgcagt taattaagct gcgagtgcca gttcttgatt ggcagttaca ttttcccgga
      901 ggatttacga gaccccgaga gctcgacatg ccccgctgac cttagtatcc ccgtcgattc
      961 cggtcgcccc ctttcccatt tcacggttta catcgctcat tctagaggct catcataccg
     1021 cagaggtcaa agagatgtca taataaacgc aaaagaaaag agggcatact cgtgagctgg
     1081 agtatgccct cccttctttg tagaggtacg atccgtagat cgtatgtgct atctgacggc
     1141 tgattttagg attttccctg ccgagaactt cggaaccttg gcagcgggaa tccgcagcgc
     1201 ctcaccggtt ctgggattcc ttccaagccg ggctttccgt ttggcaacgg taaaggtccc
     1261 aaaattcacc agcgagacac gttgtccctt tttcagggac gtcgtcaccg ctccggtgaa
     1321 agcctgcaat gcagtgcctg ctgcaacctt ggtgatgccc gcggatgccg ccatctttgc
     1381 gatcaactct tcttttgtca tcgtgcccct cctgttattg ggttgaactg cgcgactcct
     1441 tgccaccaac gtattctgtc atcacaccca catggatcaa cgttcccagg cacgcccaag
     1501 gcgtcaagct ccacgaatct gcgtggtccg attccgtttc acaagaatgt gcaagtcatg
     1561 gatctttttc ctgagggtgt ttcgattcag tcccagtaat tctgctgctt ggatctggtt
     1621 gcctttcgtt tcctgaaggg ctctggtgat gaggggacgc tcaatcgcag cgatcaacat
     1681 gggatgcaga ttacgtcccg aaccgtttcg catgcccttc acgaagtctc ccatcttcca
     1741 ctcaagaaag ttttcaagtg acagggtgtc tttcccgttt gttcgagcag cattctgccg
     1801 cgccatcatg tgcatgaact tggaggctcg cttgagtaca gttcgagaac cggtgatgac
     1861 catggtgtgt gcgaggtcga tgtcgagggg gatggcggtg gctctatcgg atgaacctgg
     1921 tttcaattcg acaatcaccg cgtcaaacgt gcgacgcgtc acgtccttcg gcagtgcgga
     1981 cacatctttc gccacagtca cagacgcatc tttgaaggcg tgcttgaact gactctggat
     2041 gtcctgatca ctgctgagca acaaaatact gaacgcggat gatgatgtag gcgacatgaa
     2101 cattccagga gaagggcgaa gaagtggtgc ggattattgc ccagggccaa tttgatgtca
     2161 atgaggaaaa tataggtggc gcagtttttt tggtccggat acagggagca tgatggtttt
     2221 ttcttgttgg atgagcaact tacctccatg agtgaggatc gaagtatgaa tcgagaactt
     2281 ctgtataata cccggcatac ccggctgctt ccctgccatg cctatctgat acagagaggt
     2341 gtatgaatga gtcacgatct tctcggcagg tttgtccctg attagtaccg gcaaaacttt
     2401 taggactata ctctttgaac cgccagagag agatggcttg acagcagggc ctatagggta
     2461 gtattaaata ggctatgttt taattcctac cggaatactc atgtatgaag gtatctctca
     2521 gagcaaccta tggaattatg gcggcagttg acctagcgat gcagctcggg acagcgccag
     2581 tccaagcgaa atcgattgca cgtcgacaag ctattcctgc tcgatttctt gaacaggtcc
     2641 ttcacagtat gaagaaggcc ggtcttgtat cgagtcttcg cggtgctcaa ggggggtatg
     2701 tactgtcgaa gaaaccctcc gaggtatcga tcgcagagat ccttgaggcc ctcgatggtc
     2761 cgctcttacc tcctctcgga ggagctggtc gggcgcaggt gcgacggctc ggtaaaccgg
     2821 aactgttgct tgggaaggtg tgggagcagg tctatcaagc ggaacgccaa gtgcttgagg
     2881 ccataagcgt ggaaaaatta gccgggcagc aacgagtgct tgagcaagaa cggtcgttga
     2941 tgtatcacat ttgaggtcac ccatgaacca cacaccactc agtccagttt caataaagac
     3001 cgaacccatt cctcctgcca ttttggaaga gatcgagacc tttgaggccg aggccaggcg
     3061 catggtttcc ggtgacgtgt cgggcgatct gttcaggcct ttccgtctgc agtatgggat
     3121 ctacggccaa cggcaagccg gtgtccaaat ggttcgtatc aagatcccat tcggaggact
     3181 gacggcgaat cagcttcgcc ggattgcgga actggccgag cactatgcca ccggtgtcgg
     3241 gcatgtgacc acgcgccagg atattcaact gcattttgtt gaattaacgg atgtgccgac
     3301 catcatgcga gggctggccg aagtcggtgt gactaccaga gaggcttgtg ccaacacggt
     3361 ccgcaatgtg acggcctgtc accttgcagg aatctgtcag ggggaagtgt tcgacgtgac
     3421 gccctatgcc aaaaccgttg cactccacct tttgcggaat ccgctcaacc agagccttcc
     3481 tcgcaaattt aaaattgctt tctctggctg caaacaggac tgcgcgctca caccgattca
     3541 cgacatcggg cttctggccg cgaagcgtgc ggatggagcc atcggctttc gaatggtcgc
     3601 aggcggcggt ctcggttcgg cccctcggat cgcgcaggtc cttcgggaat tcactccgat
     3661 ggatgagctc atcccgagta tagaggcggt gattcgagta ttcgacacct tgggtaatcg
     3721 aaaaaaccgc acgaaggccc ggatgaaatt tgtcatcgac aagctgggtt tcgaggagtt
     3781 caaacgccgc tgggaagagg cctatgtgtc gatggggcat gcccgtccga cccatgaacc
     3841 catcaagtta ctgtcgcatc cggatgaccc tgttccgctc atcatgccga cgtcgaatgg
     3901 atccaagcag gcagggatac agactaatgg gcatgcccaa agtccgcgag aggagacgcc
     3961 gtttgagatg tggcgacgta ccaacgtggt gcgtcagaag caagacggct atgttgccgc
     4021 agtggtcaaa ttgttcatgg gcgatctcac ggcagagcag atgttgttca ttgcggatct
     4081 tgcggatcac tactccaatg gcaacattcg aacgacgatc aatcaaaaca tgatcattcg
     4141 atgggtgccg gagtcacgca tcgccgatct ccatgccgat cttacctcac agggcttggc
     4201 cgatcccggt gccgaattgg tcgaagatat tattgcctgt cccggtaccg atacgtgcgg
     4261 cttggggatc acgtcttcaa agggccttgc gcgagctctg gcagaggtat ttccggcagg
     4321 gcgggtccct gaggacctga aggatgtcag cgtcaagatt agcgggtgcc acaactcctg
     4381 tgcacagcac catattgcga cgatcggctt gcatggagtg gggaaacgca ttggcgagca
     4441 tgtcgcgcct ttctatgagt tacatttggg cgggagggtc aacgggacgg cgaagatcgg
     4501 acagatggtg gtgaagttgc cggcaaaaag cgtgtcggcg gcgatcacgc acttgattga
     4561 cgtgtatcgg cgtgaccgcc tctccgggga aggactgccg gccttcatcg atcgtgtcgg
     4621 caaggtcaag ctcaaggatg agttgatccc gtataccatc gtgccgtcct ttcaggatga
     4681 ttcgatgttc tactacgact gggaaggaga agaagaattt gtcttggagg atctcggacc
     4741 tggcgagtgt gcaggtggtg cactggaaat gatcgagaac ggcattctcg aagccgacca
     4801 agagctctat caggcgaagc tgcttgtcga gaagcatcaa tactccgtct cggtgaacaa
     4861 gtcctatcgc gcggtgttgg ccgccgctaa agccttgttg gtgacggagg gacttgaacc
     4921 gtcaactgac gcagagacct tcgtcgagtt tgacagccgg cttgcgcaaa aggggattgt
     4981 tccagcaatg tatcgagact tgagaatgcg ggtcggcgat ttagggccca aggagacaac
     5041 gggcgaatca gctcgcgaca agatggaatt tgccaaggga tttgtcgatg cctgccgtgc
     5101 cgcgacggag cagatgggga aagatctgaa attggcaccg gtgggcaagg cggcagtggc
     5161 cgagaccagc acgacaccgg aagtcaagcc ggttgtatct tctgccacga cgcctgctgc
     5221 gtccggcgta ccggtctatg acctgcgcgg ggtggcctgt ccgatgaact atgtgaagac
     5281 caagctgaaa ctcgagatga tggatgcagg cgaacatttg gaggtctggc tcgatgcggg
     5341 ggaacccatc aagaacgtgc ctctgagttt gaaaaacgac ggacataagc tgttactgca
     5401 ggaaccgctc gaagcagccg cgtcacatta tcggattctg gtcgaaaaag tggagtgaag
     5461 cggcgaagga atgtttcctt gctcgcgcaa cgcgcggcct cagaaggaga gaggggagcg
     5521 gccaggtgcc cttcttgctc acagaacgcg cacgataaga atgtgctcgt tcgatgcgcg
     5581 cagtaaaggg cagcctcggc cactccccta aagaagggta tagagaaata gaggattgga
     5641 aggccctcgc ccgggctaat ggcacgtctc ggcgtgccag tgggtgggcg ggtgagaaag
     5701 tcgcgcgcag ttggaaatca tcccctcgcc gcttcaagtg tgagtggggg agaaaagatt
     5761 ctaatggaaa gcctctcgtc caaagaactt gcagcttggg gcgaatcgtt cgaggaaaag
     5821 tcgccacaag agttactggc ggcggtgata gagcgttacg cgacgaagat cgtacttgcc
     5881 tgcagttttg gtgcggagga tgtggtgctt gtggacatgg tccaccgcat caatccatct
     5941 gtgccgctct tctatcttga tacggacttt ctctttcccg agacctatgc cacgcgcgat
     6001 cgtgtgatcg agcgttatgg gttgcagccg caacaggtca tacaggtgaa gtcgttgctg
     6061 acgccggaac aacaggccgt tcagcatggc gatgccctgt ggacgcgcca tcctgatcaa
     6121 tgttgcgagt tgcgcaaagt ggagccgctc atccgcgtgc tcaagggatt cgacgcatgg
     6181 atcactggaa tcaggcggga tcagtctccc acgagagcca atgcgaagtt gatcgagtgg
     6241 gaccagaaat ttcagttggt caaagtcaat ccgcttgcga agtggacgtg ggaggatgtc
     6301 tggacccaca ttcgagtcca cgaagttcct tacaatgaat tgcataaccg caactacccc
     6361 agtatcggct gcacccattg cacgagcccg gtgatgcccg gtgaggactc ccgcgcgggc
     6421 cggtggaaaa atttttcaaa aacagaatgt ggattacaca aggcctcgtg atgtcgatgc
     6481 aggaatttat cgcaaagatt ggcaagggcc caaagactgc caaggatctc acctgggacg
     6541 aagcaaagcg ggcgatgaag gtgctcattg aaggagaggc cacgccggca caggtgggcg
     6601 gcttcctcat ggctatgcgg atcaagatgg agtctgtcac cgagctggct tcaatgaccg
     6661 caacggcgcg ttcctatgtg gcgcctgtgc caatcccacg agaactcggc gttgtggatc
     6721 tgccgagtta cgcagggaaa caggaaacct tccatgccct tgccgccgct gccatcgtag
     6781 ctgcgtcggc aggcgcgtcg gtcttgatgc atgggtatga cgggattcct ggccgcccag
     6841 ggaatgccgg ggcgctcaag accttggggc tgccgattga cctagagccg aaagcggtgg
     6901 cagaaa
//
LOCUS       DATIPF010000302         4520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1288874, whole genome shotgun sequence.
ACCESSION   DATIPF010000302 DATIPF010000000
VERSION     DATIPF010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288874"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VLL06_07520"
     CDS             complement(<1..235)
                     /locus_tag="VLL06_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30850.1"
                     /translation="MRHIFFAVFLVVACAAVAPAASIEEAKSAYDRGDYTQAARLYAP
                     LAEQGIAAAQFNLGVMYRYGEGFPQETQEVVKWY"
     gene            420..659
                     /locus_tag="VLL06_07525"
     CDS             420..659
                     /locus_tag="VLL06_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30851.1"
                     /translation="MKIRITVNQSADRSPCAHRDLTLAGRSTRLQGLALKRSTERYTC
                     NACGKPLVRTFKTSWSYQPVPGHQTTETYPSDTAN"
     gene            complement(714..1325)
                     /gene="thpR"
                     /locus_tag="VLL06_07530"
     CDS             complement(714..1325)
                     /gene="thpR"
                     /locus_tag="VLL06_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HSS30852.1"
                     /translation="MIRAFLAVELSEELRATLATLQQELKQSIEPDLKRGTRISWVQP
                     ASLHLTIKFLGETDEQVIDPLRAAVEQAIGRQMAVNVPLERLSGFPRPQSPRVLWVGP
                     SENWEKGIDAKRITEIYATIEQACEGLRFLRETKPFNPHLTVARIKTGERHVGVSLAQ
                     SGVLDRPILLGSLAVESVALMKSELKPTGSVYTKLWSVRLSGK"
     gene            complement(1322..2623)
                     /locus_tag="VLL06_07535"
     CDS             complement(1322..2623)
                     /locus_tag="VLL06_07535"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HSS30853.1"
                     /translation="MSKASVPLLLTAETIAIGTELLIGGKSDFNSLFLADELGTLGIP
                     VRFKSVVGDERPDIVMAIHTAVKRAQVIVMTGGLGPTVDDCTREAVAQATGHRLCRRK
                     DALEGMTARLAQWGRRPNKGQLRQAMIPSGATVLKNPVGSAPGFCLRWKKAFIITLPG
                     VPREMEEMMRQEVLPLLRAEYGASGRSLRAPIIRQVFHTFGLPEADVDTKLTGLIPKG
                     VPVDLGLLASPMGVLVSLTTKGSQSAHRTDRDLLPKLADDLRSRLTDWLFAEGRDTME
                     EVVGRALTKQGLMLAVAESCTGGLIGHRLTQVAGSSAYMDRGAICYSNRAKTEMLGVP
                     PELIGRWGAVSKEVAAAMACGIRSRANVSVGLSVTGIAGPGGGTETKPVGLVYIGLDD
                     GTGRPTAQEFRFHGDRNVIKQRSSQAALDLLRRWLLDRASK"
     gene            complement(2826..3461)
                     /locus_tag="VLL06_07540"
     CDS             complement(2826..3461)
                     /locus_tag="VLL06_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30854.1"
                     /translation="MIWYCHSMGVMQAHSQKRMLWYMGTVALSVLSHVSAYAIDVHPT
                     AKQIKTAIEQGNAAAEMRTTPESFYVWFGVSDAVTPRGFLVTKLGALSVMVSHMALRG
                     HQPSESDVRQVLESQTMLVSTVIFGNAPNFAVDSYIVLEQSGKTIKPVTVRFDAQADR
                     SVVYPKTPRFKAKVVGSFNYADFDPLAETTITVYPATGGEASFSMNFLDIH"
     gene            3720..4442
                     /locus_tag="VLL06_07545"
     CDS             3720..4442
                     /locus_tag="VLL06_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HSS30855.1"
                     /translation="MPTPEQTPAPQAPKRQYINFAFYKIDPAWRRLPESERTKGKQEF
                     MRAVEDYTGKVLVVPYSSIGIRGDCDIMLWRIGYELELFQDMTTKILASGLGQYLTTP
                     YSYLSITKRSVYVDHHTHENQESKRLTVVPGKAKYIFVYPFLKTREWFLLTKAARQGM
                     MDEHIEVGHRFPSVKLNTSYSFGLDDQEWVVAFESDKPEDFLDLVMALRETEGSRYTL
                     RDTPIFTCIRKSLKEMLDTLGG"
BASE COUNT         1013 a         1371 c         1200 g          936 t
ORIGIN      
        1 ggtaccactt cacgacttcc tgagtctcct gagggaagcc ctctccatag cgatacatca
       61 cacccaaatt gaactgcgct gctgcgattc cttgctccgc gaggggagcg tagagccgtg
      121 ccgcctgtgt atagtcacca cggtcatagg cggatttcgc ttcctcaatg gaagctgctg
      181 gtgcaactgc cgcgcatgct acgaccaaga acaccgcaaa gaagatatgc cgcatggttc
      241 acctacttct caatcgtgac aacagagcgt cggaaccgag gtgcaatacc ctgtcacagc
      301 tcgccgagat tggcaaatgt tatgaatggg ggaaaactgt tgccggctga tcgcagaccg
      361 ctgccatagt tgaagaaagg cacggagatt tgttacaagg taggcatgag cgaacaccga
      421 tgaagatacg cattacggtg aaccagtcag cagatcgctc accctgtgcc catcgagacc
      481 tgaccttggc agggcgatcc acgagactcc aagggcttgc actgaagcgt tcgacggaac
      541 gctacacatg caatgcgtgc ggcaaaccgc ttgtccgcac gttcaaaaca tcctggtcct
      601 atcagccagt ccccggtcat cagaccactg aaacgtatcc atccgacacc gcgaattgat
      661 ttcactggac acaggaccta tccaccttgt tacgccccac gtcactacgg aactcatttc
      721 ccgctcagcc gcacgctcca caacttcgta tagacggaac cggtcggctt caattcactc
      781 ttcatcaatg cgaccgattc cacggccagc gatcccaata aaatcggacg atccaacact
      841 ccgctttgtg ccaatgagac tcctacgtgc cgctcccccg ttttgatccg cgccacggtt
      901 agatgaggat tgaacggttt ggtctcgcgg agaaagcgca agccctcaca agcctgttcg
      961 atcgtggcat agatctccgt gatacgcttc gcatctattc ccttctccca attctccgac
     1021 ggcccaaccc atagtacgcg cggactttgc ggacgaggaa agccgctgag tcgttcaagc
     1081 ggcacgttca cagccatctg ccttccgatt gcctgctcaa ccgcagcgcg cagtggatcg
     1141 atcacctgtt catctgtctc gccgagaaac ttgatcgtca gatggagtga cgccggctgc
     1201 acccaggaga tgcgtgtgcc tcgcttcaag tccggctcaa tactctgctt caactcctgt
     1261 tggagagtgg caagcgtggc ccgaagctct tcagagagtt cgacggcgag gaacgcccgg
     1321 atcatttcga tgccctatcg agcaaccagc ggcgaaggag gtctagcgcg gcctgtgaag
     1381 accgttgttt gatgacgttc cgatcaccgt gaaaccggaa ctcctgtgcg gtcggtcggc
     1441 cggttccgtc atccaacccg atatagacca gcccgaccgg cttggtctcg gtcccgccgc
     1501 ctggcccggc aataccggtg acactgagtc ccaccgagac gttggcgcga ctgcgaatgc
     1561 cgcaggccat cgcggccgcg acctccttgc tcactgcgcc ccatctgcca atcagttccg
     1621 gcggcacacc cagcatctcc gtcttcgccc gattgctgta gcagatggct ccacgatcca
     1681 tgtaggctga tgaaccggcc acttgcgtga gtcgatggcc gattaatccg ccagtgcagg
     1741 actcggcgac cgccagcatg agcccctgtt tggtgagtgc ccgcccgaca acttcttcca
     1801 tcgtgtcacg cccttcggca aagagccagt cggtgagtcg cgaacgaagg tcgtcggcca
     1861 gcttcggcag cagatcacga tctgtcctat gggccgattg actccccttc gtcgtcagcg
     1921 agaccagcac acccatcggc gatgcgagta accccagatc taccggcacc cctttgggaa
     1981 tgagaccagt taatttggta tcgacgtccg cctctggcag gccgaaggta tgaaatacct
     2041 gacggatgat cggcgctcga agagaccggc ccgacgcgcc atactctgcg cgcagcagcg
     2101 gcagcacttc ctgccgcatc atttcttcca tttctcgcgg cacgccaggc aaggtgatga
     2161 taaacgcctt cttccatctc aggcaaaatc ccggcgcaga gccgaccgga ttcttcagca
     2221 ccgtggcacc ggacggaatc atcgcctgcc gcagctgtcc tttgttgggt cgtcgtcccc
     2281 actgcgcgag tctggccgtc atcccttcca gcgcgtcctt gcggcgacag agccggtgtc
     2341 cggtcgcctg ggccaccgcc tctctcgtgc aatcgtccac ggtaggaccg agtcccccgg
     2401 tcatgacgat cacctgggct cgcttcacgg cagtgtgaat ggccatgacg atatccggcc
     2461 gttcatcacc gacgactgat ttgaatcgaa cggggatacc gagcgtgccg agttcgtcgg
     2521 ccaggaagag agagttgaaa tcggattttc cgccgatcag cagttcagtc ccaatcgcga
     2581 tggtttctgc cgtgaggagc agaggtacgg atgctttgga catggatagc aggaagcggt
     2641 cctagcccac attattcatg aaggttcgtt gcgaaagcgt gcagacgcaa agcgagacgg
     2701 gcactcggaa gcgtgaagcc ctgaggcata ctcgggcagt atgtcgaagg gctgaacggc
     2761 gagactgctc gtcacagccg cgtatccgcg cttgtagcag aagcttcatg aataatgtgg
     2821 gctgactagt gaatatcgag aaaattcatg gagaagctgg cctctcctcc ggtcgccgga
     2881 tacaccgtaa tcgtcgtctc ggccagcgga tcgaaatcgg cataattgaa cgaccccacg
     2941 accttcgcct taaaccgagg agtcttcgga tacacgacgc tgcggtcggc ctgtgcatcg
     3001 aatcgcaccg tcacgggctt aatagtcttc ccactctgtt cgagcacgat gtaactgtcc
     3061 accgcgaagt tgggagcatt cccgaagatg acggtgctga ccagcatagt ctggctttcc
     3121 agtacttgcc tcacatcact ctcactcggt tggtggcccc tcagagccat atgcgagacc
     3181 atgaccgaca gggctcctaa ttttgtgacc aagaatcccc tgggagtgac cgcgtcgctc
     3241 actccgaacc acacatagaa actctccggt gtggtccgca tctctgccgc ggcgttcccc
     3301 tgctcgattg cagtcttaat ctgcttcgca gtgggatgca cgtcgatggc ataggccgac
     3361 acatgggaga ggacactcaa cgcaaccgtc cccatgtacc acagcatgcg cttctgacta
     3421 tgggcctgca tgacccccat actatggcag taccagatca tccttgttgt gtcaacgaac
     3481 ggccgagttc ccggtagtgg gtaataaccc actaccccag ttcccttcac ggttgacaat
     3541 aacaaaggtg aagtgataaa aaaccgagcc gttacggggt cagagttcgc tatggctcca
     3601 gccgcgagac gctcgagact gaagcccgcc tggacctctt gcattacgac cgacgtcgga
     3661 gctatgcatg aataatccgg gttagtgtcc ttttcaagaa gttatacgga ggagtttcga
     3721 tgccgacacc tgaacaaacc ccagctcccc aagcacctaa acgccaatat atcaacttcg
     3781 ccttctataa aatcgacccg gcctggcggc gcctccccga gagtgaacgc accaaaggta
     3841 agcaggaatt catgcgggcg gtcgaagact ataccggcaa agtccttgtg gttccgtact
     3901 cttcgattgg aatcaggggc gattgcgaca tcatgctatg gcggattggt tacgaattgg
     3961 aactcttcca ggacatgacc acgaaaatct tggcctcggg gttgggacag tacctcacca
     4021 ctccctactc ttatctctcg atcaccaagc gctcagtcta cgtggatcat cacacccacg
     4081 aaaatcaaga gagtaagcgt ctgaccgtcg ttccgggaaa agccaagtat attttcgtct
     4141 acccgttcct gaaaacgcgc gaatggttcc tgctcacgaa ggccgcgcgg caaggcatga
     4201 tggacgagca catcgaggtc ggccatcgct tcccatcggt aaaattgaac acgagctatt
     4261 cattcggctt ggatgatcag gaatgggtgg tggcgtttga gagcgataag ccggaagact
     4321 tcctggatct tgtgatggcg ctccgcgaaa ccgaaggctc ccgctatacg ctgcgcgata
     4381 caccgatttt tacctgtatt cgcaagagcc tcaaggaaat gctcgacacg ctcggcggct
     4441 gacacggcat caaagagaca cacacaacaa ggccttcggt cggaagaccg aaggccttgt
     4501 tgttgagaga gcaggcaccc
//
LOCUS       DATIPF010000303         5006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1384855, whole genome shotgun sequence.
ACCESSION   DATIPF010000303 DATIPF010000000
VERSION     DATIPF010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384855"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VLL06_07550"
     CDS             <1..215
                     /locus_tag="VLL06_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30856.1"
                     /translation="VAHLADVVAAASTWMEKYVIYGGLNVIGYGNHLAARQWRQLQSG
                     MVHHYAAIIVAGLFLLAVIIQLIMQL"
     gene            225..1784
                     /locus_tag="VLL06_07555"
     CDS             225..1784
                     /locus_tag="VLL06_07555"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HSS30857.1"
                     /translation="MLEELASTFPILTCILFLPVIGAVVLWLFDDEDMVRISALAVAL
                     IELVLSVFVLIRFTPESAAMQFSEHVPWIPTLGISYHLAVDGISVLFVGLTAFLTVLI
                     VIYSWDTVRHQVKLYMMALLALETTTMGVFLSLDLILFFVFWELMLIPSYFLIKLWGG
                     GAERHYAALKFVLYTLLGSVFMLVGIVLLDINYHQWATLRHIEPTYSFDLLELLSVPI
                     PLGQQVLIFWLLFLGFAFKAPVFPFHTWLPDALFDGPSGMAVVLAGLKLGTFGFIRFS
                     IPLLPDASKSHTVVTIIMVLGLAAILYGGIMALIQQDFRRLLAFSSISHLGFVVIGLF
                     ALNYQGLQGSLLTMINLGFSTAGLFFIAGFLYSRQQSTQLGSFGGMAKQTPLLASYFL
                     FLGLASIGLPGTNGFVGEFLILLGTFKANWLYGGIAVTGVIFSAAYFLWYYERSMLGP
                     IGKSVKDSISDLHPRELLIAVSLSVMILWIGLYPSPFLKMMNGSVQALVDRLERGTVA
                     SIESAQPVRVD"
     gene            1790..3502
                     /locus_tag="VLL06_07560"
     CDS             1790..3502
                     /locus_tag="VLL06_07560"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HSS30858.1"
                     /translation="MGDYTLLYILFAPFVGAIALIFVSNRQAMLVRGIATGSALVSLL
                     ASLYLFFAYDHVTGGFQFVNKFEWSRQLGISLHLGVDGIGTPLVLASSILLFAGIFVS
                     WHIKDRAKEFYIWLLILAAATIGVFMSLDLFFLFFFYEMSVIPMYLLLGMWGSHTKKY
                     NEMTDPEGIKQRDSVGFILNFESNSKEYAAMKLVLFLSAFAVVALMGILLIYKYSGLN
                     TFDILVLREHAKVFMNIPVLGTTLDKIIWLLIFFGFASIAPLWPLHSWSPVGHAAAPA
                     ATSMLHAGVLMKLGHFSIIRVAFEILPETTRELMPIAAVLCMFSIVYGGFVAFYAKDT
                     KYVIGYSSSSHMGYVFLGMAALDYISLTGAVMYMFAHAMATGMLFAMAGWVYDQTHSR
                     DIPSLGGLSNRMPFISGCFVIACMASVGVPGTVNFIAEVMIIVGSWNKYPFQVIVAVV
                     GIVLTLAYLFKMMRGLFYGPMDQKYSHAHDAVAVVDRLPLLIMITVSIGFFFFPMHLY
                     NVVRSGVDPLIAKIIRVVPVAGLDRETVEFTRDTTNTPGTPDALLLTNTSSPLAPTSS
                     RGLE"
     gene            3499..5001
                     /locus_tag="VLL06_07565"
     CDS             3499..5001
                     /locus_tag="VLL06_07565"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HSS30859.1"
                     /translation="MTFVLPMSSSDLLLLLPEILLTSWLCLLLIIDFSFPRLPNEQLA
                     YFSIAGLVATLGCLAWFDYTGVTGTLFSNMFALDRMALFFKMFIVGSTILVILASIDY
                     IHRFKFFRGEYYFLTVMSALGMMFMASANDLLSVFVALEFSTLGFYVLVAYLREDTAS
                     NEAGIKFFILGIFAAGLLAYGISLVYGETGKLVFSDMTAAQATPGLVIGFLLIFGALG
                     FKIGAVPFHSWIPDTYHGSPTPVTAFLSIAPKAAAFAILLRMFFVALATFKPIWALLL
                     IAASILSMTYGNIVAIAQKNMKRLLAYSGIAQIGNILIGLATGTKMGSDALLFYLVTY
                     LFANLGAFAIVIAVSEAIGSEEIDDYSGLGRRSPFLAFSMLIFLLSLAGVPPLAGFIG
                     KLYIFVAAIKEGLYLLIAVGLINIVISMYYYLIVVKKMYVTEPHDRSPVKVSGPIKVV
                     VYVCLIGTLVIGVYPQPVTDWVVSAILIFSHFSDPTASLPVPPAVIPLGG"
BASE COUNT          893 a         1432 c         1284 g         1397 t
ORIGIN      
        1 ctgtcgcaca cttggctgat gtcgtcgcgg ccgcttcgac ctggatggaa aaatatgtga
       61 tctatggcgg gctgaacgtg atcggatacg gcaaccatct tgccgcccgt cagtggcggc
      121 aactgcagag cgggatggtg catcattacg ccgcgatcat tgtggccggg ctcttcctgt
      181 tggcggtcat tattcagctc atcatgcaac tataacgcac ggtcatgtta gaagaactgg
      241 catcaacgtt tccaattctc acctgtattc tttttcttcc cgtcatcggg gccgttgtcc
      301 tgtggctgtt cgacgacgag gacatggttc ggatctccgc gttggcggtt gcgctcatag
      361 agttagtcct ttccgtattc gtcctcatac gattcactcc agaatcggct gccatgcagt
      421 tttccgaaca cgtgccatgg attcccactc ttggaatcag ctaccacctc gcggtcgacg
      481 ggatcagtgt cctcttcgtc gggctcaccg ccttcctcac ggtcctgatc gtgatctatt
      541 cctgggacac cgttcgccat caagtgaagc tctacatgat ggcactcctc gcacttgaaa
      601 cgaccacgat gggggtcttc ctctctcttg acttgatcct cttcttcgtt ttttgggagc
      661 tcatgctcat ccccagctat tttctgatca aactctgggg tggaggagcg gagcggcact
      721 acgccgcact gaaattcgtg ctctatacct tgcttggtag cgttttcatg ctggtgggca
      781 ttgtcctctt ggatattaat tatcaccagt gggcgacgct acggcatata gagccgacgt
      841 actcatttga cttattggaa ctgctgtccg ttccgattcc gctcggccag caagtgctca
      901 ttttctggct gttgttcctc ggatttgcgt tcaaggcccc agtgttccct ttccacacct
      961 ggctcccgga cgctctcttc gacgggccga gcggcatggc ggtggtgctg gccgggctga
     1021 aactcgggac gttcggcttc atccggttta gcattccgtt gctcccggac gcatcaaaaa
     1081 gtcacaccgt cgtcaccatc atcatggtgc tgggattggc ggcgattctc tatgggggga
     1141 ttatggcgct gatccagcaa gattttcgcc gcctccttgc gttcagcagt atcagccacc
     1201 tcggattcgt agtgatcggc ctgttcgccc tgaactatca aggactccaa ggcagcttgc
     1261 tcaccatgat caacttggga ttcagcacgg cgggtttgtt ctttattgcg ggttttctct
     1321 attcacggca gcagagtacc caactgggct catttggggg gatggcgaaa cagactccct
     1381 tgctcgcaag ctatttcttg ttcctcggat tggcctcgat cggtttgccg ggcaccaatg
     1441 gcttcgtcgg tgaattcctg atcctcttgg gaacgttcaa ggccaattgg ctgtacggtg
     1501 ggattgcggt caccggtgtc atcttcagcg cggcatattt tctctggtac tacgaacggt
     1561 cgatgctggg accaatcggc aaatccgtca aggattcgat cagtgatctc catccccgcg
     1621 aactgctcat cgcggtgtct ttgtccgtga tgatcctctg gatcggcctc tatccctctc
     1681 ccttcttgaa aatgatgaac ggatccgtgc aggcgttggt ggatcgactt gaacggggaa
     1741 ccgtggcgtc aatcgagtcc gcacagccgg tcagggtgga ttaattcgca tgggtgacta
     1801 cacactgctc tatattctgt ttgccccctt cgtgggggcc atcgcgctga tctttgtctc
     1861 caaccggcaa gcgatgcttg tgcgaggcat cgctacggga tcagcccttg tctccctgct
     1921 tgcctccctc tacttattct tcgcctacga tcatgtgacg gggggattcc aattcgtcaa
     1981 caaattcgaa tggtctcgcc agttgggcat ctcgctccac ctcggggtgg atgggatcgg
     2041 gaccccgctg gtgctggcat cgagcatctt gttgtttgca ggaattttcg tctcttggca
     2101 catcaaggat cgggccaaag aattctatat ctggttgctc atcctcgccg ccgccacgat
     2161 tggcgtcttc atgtcgttgg atctgttctt tctcttcttc ttctacgaaa tgtccgtgat
     2221 ccccatgtat ctcctgttgg gcatgtgggg aagccacaca aaaaagtaca acgagatgac
     2281 ggatccggaa ggcatcaaac agcgggattc ggtcgggttt atcctcaact tcgaatccaa
     2341 cagcaaagaa tacgccgcga tgaagctggt gctctttctt tccgcctttg ccgtcgtggc
     2401 gctgatgggt atattgctga tctacaagta ctcggggctc aataccttcg acattttggt
     2461 cctgcgtgaa cacgccaaag tattcatgaa cattccggtc ttgggtacga ccctggacaa
     2521 gatcatttgg ctactgattt tcttcggctt tgcctcgatc gcaccgttgt ggccgctgca
     2581 ctcttggtct cctgtgggcc acgctgccgc gccggccgcc acaagtatgt tgcatgcggg
     2641 tgtgctgatg aagctcggcc atttttccat catccgggtg gcgtttgaga ttctgccgga
     2701 gaccaccaga gaactcatgc ccatcgcggc cgtgctctgt atgttcagta ttgtgtatgg
     2761 cgggttcgtt gcgttctatg cgaaggatac caagtatgtc atcgggtatt cgagttcaag
     2821 tcacatgggc tatgtcttct taggcatggc cgcgttggac tacatcagcc taaccggcgc
     2881 ggtgatgtat atgtttgccc atgctatggc cacgggcatg ctctttgcca tggccgggtg
     2941 ggtctacgac cagacgcaca gccgcgatat tccttcgctc ggcgggctct cgaatcgcat
     3001 gccgttcatc tccggctgtt tcgtgattgc ctgcatggcc tccgtcggtg tgcccggcac
     3061 cgtgaatttt atcgccgaag tcatgattat cgtcggcagt tggaacaagt atcccttcca
     3121 agtcatcgtg gctgtggtcg gtatcgtgtt gaccttggcc tacctgttca agatgatgcg
     3181 cgggctcttc tacggaccca tggaccagaa gtatagtcat gcgcacgacg ccgttgctgt
     3241 ggttgatcgt cttccgctct tgatcatgat caccgtcagc atcgggttct ttttctttcc
     3301 catgcatctc tataatgtgg tgcgttctgg cgtcgatccc ttgatcgcaa agatcatccg
     3361 cgtcgttccg gtggctgggc tcgatcgtga aaccgtagag ttcacacgtg acacgacaaa
     3421 tactccgggg acgcctgatg ctctgctgtt gacgaacacc tcctcccctc tcgcccctac
     3481 ctcctcacga gggctcgaat gaccttcgtg ctaccaatgt cctcctccga tctcttgctg
     3541 ctactaccgg aaatcctgct cacgtcctgg ctctgtcttc tcctgatcat tgatttctcg
     3601 ttccctcggc ttcccaacga gcaattagcc tacttcagca ttgcggggct cgtcgccacc
     3661 cttggctgtt tggcttggtt cgattacacc ggcgtcaccg ggacgctctt ctcgaatatg
     3721 ttcgccctcg accggatggc cctcttcttt aaaatgttca tcgtcggatc aacgatcctc
     3781 gtcattctgg cctcgatcga ttacatccat cgattcaagt tcttccgtgg agagtattat
     3841 ttcctgaccg tgatgtcggc tctcggaatg atgtttatgg cttccgcgaa tgatctgcta
     3901 tcggtcttcg tggccctgga attttccacc ctaggattct acgttctcgt tgcgtatctc
     3961 cgcgaggata cggcctcaaa tgaagcaggt atcaagtttt ttatcttggg tatcttcgcc
     4021 gccggcctcc ttgcctacgg catcagcctg gtctatgggg aaaccggcaa gctggtattc
     4081 tctgacatga cggcggcaca ggccacaccg ggattggtca ttggtttcct cctaattttc
     4141 ggcgcattag gattcaaaat cggggccgtg cccttccact catggattcc agacacctat
     4201 catggatcgc cgacaccggt gacagccttt ctttcaattg ccccaaaggc agcggctttc
     4261 gccatcctcc tcagaatgtt cttcgtcgcc ttggcgacct ttaagccgat ctgggccctg
     4321 ctgcttatcg cggcgtccat cttgtccatg acctacggca acattgtggc catcgctcaa
     4381 aaaaacatga aacggctctt ggcctattcc ggtattgctc agatcggcaa catcctgatt
     4441 ggtctcgcaa ccgggacgaa gatgggcagt gatgcgctgc tgttctacct cgtgacctat
     4501 ctgttcgcca acctcggtgc ctttgccatt gtcatcgccg tcagcgaagc catcggtagt
     4561 gaggaaatcg atgactacag cggcctggga cgccgatcgc cgtttctcgc cttttcgatg
     4621 ctgatttttc tcttatcact cgccggggta cccccgttgg ccggctttat cggtaagctg
     4681 tatatttttg tcgcggccat aaaagaaggg ctctatctgc ttattgcggt tgggctcatc
     4741 aatatcgtga tttccatgta ctactacctc attgtcgtga agaagatgta cgtgaccgag
     4801 ccccatgacc ggtcgcccgt caaggtctcg ggtccgatca aagtggtcgt ctacgtctgt
     4861 ctcatcggca cactcgtgat cggggtctat ccgcaacctg tcacggactg ggttgtttcg
     4921 gccatcttga tattttcaca cttctcagat ccaaccgcat cccttcccgt tccccctgct
     4981 gtcatccccc tcggaggcta aggcca
//
LOCUS       DATIPF010000304         6191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_147622, whole genome shotgun sequence.
ACCESSION   DATIPF010000304 DATIPF010000000
VERSION     DATIPF010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147622"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VLL06_07570"
     CDS             complement(<1..327)
                     /locus_tag="VLL06_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30860.1"
                     /translation="MRTLVTLLFLGVYLLFDNTVPAQMPIDGQSQADVLKLMGELPPE
                     TMKNVETLGKILQQDLKEGKLTQTRIQEELKSGTFEQTLRNINPEAGPLLDDIGQQMK
                     NHPNAEN"
     gene            complement(654..2075)
                     /locus_tag="VLL06_07575"
     CDS             complement(654..2075)
                     /locus_tag="VLL06_07575"
                     /inference="COORDINATES: protein motif:HMM:NF039904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HSS30861.1"
                     /translation="MSELSHQSTAPYDGPALIADPIHQYVSFTVPFSIADSHERTEKD
                     LIDSPWVQRLRYIYQLQSARWVYPSAEHTRFVHSLGTMHVAGRFAQHLYPFLKKAVKE
                     LPSANYVEEFLRVTALVHDIGHGPFCHFFDDNYLHAFHASHEKLGQIIIRDHLGPIIR
                     KIRRSPSGPFAKGEELNPDQIAHVILKEKGKDNSRIPRWLNMLQPVISGSYTADNLDY
                     VLRDSYMCGVAVGPVDLSRLIHYTIITDKGFTIHKTGLPALQMFLNTRMYLYSNVYYH
                     RTTRAIDIHLRDIFGDTMKLLFPHDPRKKMDEYLTLTDWSLLEQVRGWKTSRHTTERR
                     LSAEWQRILVRDVKWKMAYSAVLKEKGQERGMDFPSHAQFEEQIKKELPARLQKVEFR
                     VDMAPLDPRPDPKDRRGSPLYVYDPSTKGISTEPLEEFLDLLPTRLVQFRIYSLDHQH
                     DEALSRAAATVLNKTPSSFESNF"
     gene            complement(2090..2578)
                     /locus_tag="VLL06_07580"
     CDS             complement(2090..2578)
                     /locus_tag="VLL06_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HSS30862.1"
                     /translation="MATIPSFRSLLKQVLHLQESPQRTALAFAIGVFIGFSPVYGLHT
                     VMVLFLSWAFGLNLLALMVGAFLNNPWTLVPILGATYWVGALLLGRSETPSFDWHDVS
                     FGAIYEQVMPYAVPFFVGGFVLSLLGAALAYPLAYYVVAKYRQSHPPHQTAPLPPQQD
                     VR"
     gene            complement(2600..3655)
                     /gene="thiL"
                     /locus_tag="VLL06_07585"
     CDS             complement(2600..3655)
                     /gene="thiL"
                     /locus_tag="VLL06_07585"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HSS30863.1"
                     /translation="MASTVRPMARPTIHEFDLIRALHRRHGRRSPSVIQGIGDDAAVI
                     TSQADKWTVLTTDLLTEGIHFDLRTATMADIGFRAAAANLSDIAAMGATPQYLLVSLA
                     IPRTGTSRHVYQLYRGMMAACRPHHVGLIGGDTSASSSGWFLSVTLIGIVPPRKALFR
                     SGARIGDFLYVTGTIGDALAGLRLLNEPSPGTKRDPGTATLSIKHRQFLIERHLRPTA
                     RIAEGQWLSTHRLATSAMDISDGLSGDLRHICEESHVGADIDLSALPLSPACRAYATS
                     RKLDPVYIALTGGEDYELLFTVSPRQRQRLERTAIEKGFSLTCIGTIHPLRFGMQALS
                     SRGQHRRLINTSYEHFT"
     gene            complement(3838..>6191)
                     /gene="lon"
                     /locus_tag="VLL06_07590"
     CDS             complement(3838..>6191)
                     /gene="lon"
                     /locus_tag="VLL06_07590"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HSS30864.1"
                     /translation="GNRMIFLATQKALDVENPSPEDIHAVGTVGIIMRMLKLPDERIK
                     ILVQGLSKAKIAGYIQAEPYYSVRIDKLLEHKPASTALETEAAMRTVKEQIERIVSLG
                     KVLIPDVMIVIENLEDPGRLADMVASNLGLKVEVTQAVLEVIDPIKRLRHVSEILGKE
                     VEVLSMQQKIQAQAKGEMDKTQREYFLREQLKAIQKELGELDERAEEITEFRKRIAEL
                     KMPEKVLKETEKQLKRLEKMHPDTAESATVRTYIEWIVELPWSKKSKDNLDLKAAAKV
                     LNEDHYDLEKVKERILEYLAVRKLKDKMKGPILCFVGPPGVGKTSLGKSIARALGREF
                     VRISLGGVRDEAEIRGHRRTYVGALPGRIIQGMKQAGTNNPVFMLDEVDKVGMDFRGD
                     PSAALLEVLDPEQNNSFTDHYLGVPFDLTEVMFITTANLIDPILPALRDRMEVIGIPG
                     YTEEEKLGIAQKYLIPRQLTEHGITEKHVRIAEPAVRQIITNYTREAGVRNLEREIAN
                     VMRKVAKKVAEGKGVGFPVNPANLHTYLGVPKFVPEEELETDEIGVATGLAWTESGGD
                     VLYIEATAMKGKGQLTLTGQLGDVMKESAQAALSYVRSRERTLGINPDVFTSQDLHIH
                     VPAGAIPKDGPSAGITMAIAIASTLSQIPVRRDLAMTGEITLRGRVLPIGGLKEKLLA
                     AKRAKLTTVILPKRNKKDLDEIPKHILRGIRLVFADTMDDVMKVALRRGSKSTRQTGK
                     PHTPQPHSRKHATRAKTGRAARSLPVHAATTATPSLPHPLAGY"
BASE COUNT         1219 a         1587 c         1922 g         1463 t
ORIGIN      
        1 gttctctgcg ttgggatggt tcttcatttg ctgaccgatg tcgtccagca gagggccggc
       61 ttcgggattg atgtttctca acgtctgttc gaaagtgccc gacttcagtt cctcctggat
      121 tcgtgtttgg gtcagcttgc cctccttcaa atcctgttgc aggatcttcc ccaaggtttc
      181 aacgttcttc atcgtctcag gcggcagttc gcccataagt ttcagaacat cggcttggct
      241 ctgtccgtcg attggcattt gtgcggggac agtattgtcg aataatagat agacccccag
      301 gaaaagcagt gtgacgagtg tccgcatgtt catcctccgt gcagatggtg gtgtcgcatt
      361 tgaggcgatg tcaagagtat tacagtgcag tggcaggctg tcagcaaaat ctagtagttt
      421 gagcactgat cctccatatc cacttagggt tatgtcggtt ggctgagcga acgctgccga
      481 aggagcgggg tgcccaccga ggcttggccg agaggaaagc tggaactgac tgaagctctg
      541 cgatcgttag atcgcagctg aaggaattcc ggaaggtttc cgctcggccg agcccggtag
      601 ggccgctctg agaggcacgg aagctaagct gcccggcaca gcccgccatc cttctaaaaa
      661 ttcgactcga agctcgacgg ggtcttgttg agtactgtgg cggcagcgcg cgagagggct
      721 tcgtcatgct ggtggtcgag cgagtagatg cggaattgga cgagtctggt cggcaggaga
      781 tcgaggaatt cctcaagcgg ttcagtcgag atgcctttgg tgctggggtc atacacatag
      841 aggggactgc cgcgccggtc tttcgggtct ggccgtggat cgagcggtgc catgtcaacg
      901 cggaactcta ccttttggag ccgcgccggc aattctttct tgatctgttc ttcgaactgc
      961 gcatggctcg gaaaatccat gccgcgttcc tggcccttct ccttgagcac ggcgctgtag
     1021 gccatcttcc atttgacgtc gcggacgaga atgcgttgcc attctgcaga gaggcgccgc
     1081 tcggtggtgt gacgggaggt cttccatccc cgtacctgtt caagtaagga ccagtcggtc
     1141 agtgttaaat actcatccat cttcttgcgg ggatcgtggg gaaacaggag cttcatggta
     1201 tcgccgaaaa tgtcccggag atggatgtcg atcgcgcgcg tggttcggtg atagtacacg
     1261 ttggaataga gatacatgcg ggtattgagg aacatctgca gggcgggaag gcccgtctta
     1321 tgaatcgtaa atcctttgtc cgtgatgatc gtgtagtgaa tgagtctgga gagatcgacc
     1381 gggccgactg ccacgccgca catataggaa tcgcgcagga catagtcgag attgtctgcg
     1441 gtatagctgc cggaaatgac cggttggagc atgttgagcc atcggggaat gcgggaattg
     1501 tcttttcctt tctccttgag gatcacatgc gcgatttgat cggggttgag ttcttcccct
     1561 ttggcgaatg ggccggaggg gctgcgccgg attttgcgga tgattggacc cagatggtcg
     1621 cggatgatga tctgcccgag cttttcgtga ctggcatgga aggcatgcaa atagttatcg
     1681 tcaaaaaaat ggcagaaggg gccgtggccg atgtcgtgca cgagcgccgt gacgcggaga
     1741 aattcttcga catagttcgc tgacggaagc tccttgacgg cttttttgag aaatggatac
     1801 agatgttgtg caaagcggcc cgccacatgc atggtgccga gtgagtgcac gaagcgggtg
     1861 tgttccgccg aggggtatac ccagcgggcg ctttggagtt gatagatgta tcggaggcgt
     1921 tggacccaag gggagtcgat caggtctttt tccgtgcgtt catgcgaatc tgcaatggag
     1981 aagggcacgg tgaatgacac gtattggtga atcggatcgg cgatcagcgc aggcccgtca
     2041 tacggagcgg tggattgatg actcaactca gacatggcga gcatgcattt tagcgtacat
     2101 cttgctgggg cggcaatggc gcggtctgat gcggtgggtg cgattgccga tatttggcga
     2161 cgacatagta ggcaagtggg taggcgagcg ctgcaccgag gaggctcagg acaaacccac
     2221 caacaaagaa aggtacggcg tagggcatga cttgttcata gatcgcgccg aaactcacat
     2281 cgtgccagtc gaatgaaggg gtctccgatc ggccgagcaa gagtgctccg acccagtagg
     2341 tggcgccgag gatcggaacc agagtccagg ggttgttgag aaaggcgcct accatgagtg
     2401 cgagaaggtt gaggccgaat gcccaggaca agaacagcac cattacggtg tgtaatccgt
     2461 agaccgggga aaagccgatg aagactccga tggcaaaggc cagtgcagtt cgttgcggcg
     2521 actcctgcaa atggaggacc tgtttcaaca gggatcgaaa tgaagggatc gtggccatca
     2581 ccgtcgtggc tgaagctggt tatgtgaaat gttcgtaact ggtattgatc aaccgacgat
     2641 gctgtccgcg agaggacagg gcctgcatcc caaatcgaag agggtgaatt gtcccaatgc
     2701 aggtcagaga gaatcccttc tcgatggccg tccgttcgag tcgttggcgc tgtcggggcg
     2761 ataccgtaaa gagcaattca taatcttcac cgccggtcag cgcgatgtat accgggtcca
     2821 gcttccgaga ggttgcgtag gcacggcagg ccggcgataa cggaagcgca ctcagatcga
     2881 tatctgcccc gacatggctt tcctcgcaga tatggcggag atccccggag aggccgtctg
     2941 aaatatccat cgcggaggtc gccaagcgat gggtactgag ccactggcct tccgcgatac
     3001 gtgcggtcgg gcgcaggtgc cgttcgatca gaaattgccg gtgctttatc gagagtgttg
     3061 ctgtccctgg gtcacgtttc gtaccaggcg acggttcatt gaggagcctc aggccggcca
     3121 atgcatcgcc gattgtgccg gtcacataca ggaaatctcc gattcgtgcg ccgcttcgaa
     3181 acagggcctt gcggggaggg acgatcccga tcagggtcac gctgagaaac catccgctgc
     3241 tcgaagccga ggtatctcct ccgatcagtc ctacatggtg cgggcggcat gcggccatca
     3301 tccctcgata cagttgatag acatggcggc tggtgccggt acggggaatg gccaaggata
     3361 cgagcaggta ttggggggtg gctcccatcg ctgcgatgtc gctgaggttc gctgctgcgg
     3421 cgcggaaacc gatatcagcc atcgtcgcgg ttcggagatc gaagtggatc ccttccgtca
     3481 agaggtcggt ggtcagcacc gtccatttat cggcctgtga tgtgatgacg gcagcatcgt
     3541 cgccgatccc ttgaatcacc gagggagatc ggcggccgtg gcgccggtgg agggctcgaa
     3601 tgagatcgaa ttcgtggatc gtgggacggg ccatcgggcg gacggtggag gccatgctaa
     3661 cagcttcctg aaataccatc ctgggcgatg gtgacatcaa cgacgagcag atctaaattc
     3721 acgagaaaat gcctctgcgg aatatccgag cgcgacgagg tgggaatcca atccgcccac
     3781 gtcacgcttt tctcgcatgt ctcgtacgtc atgcgccgca gcctctggtg gactctttca
     3841 gtatcctgct aggggatgtg ggagggaagg cgttgccgtg gtggccgcat gcaccggcag
     3901 ggagcgagcc gctcgcccgg tcttcgcgcg tgtggcgtgt tttcttgaat gtggctgcgg
     3961 cgtgtggggc ttgcccgtct gacgcgtgga tttcgagccc cgccggagcg ccaccttcat
     4021 cacatcatcc atcgtgtcgg caaagacgag gcggatcccc ctcaaaatgt gcttgggaat
     4081 ttcatcgaga tcctttttgt tgcgttttgg aagtatcacg gtggtgagtt tggcacgttt
     4141 ggctgccaac agtttttcct tcaaccctcc gatggggagc actcggcctc gaagcgtgat
     4201 ctcgcctgtc atagccaagt cgcggcgtac ggggatttgc gatagcgtcg acgcaatggc
     4261 gatggccata gtaatgcctg ccgagggtcc gtccttggga atcgccccgg ccggtacatg
     4321 gatatggaga tcctgagagg tgaagacatc gggattgatg ccgagggtgc gctcgcgcga
     4381 tcggacatag cttaaggcgg cctgcgcgga ctccttcatc acatcgccca gctggccggt
     4441 cagggtcaat tgtcctttgc ccttcattgc cgtggcctcg atgtagagca cgtcgccacc
     4501 ggactctgtc caagctaacc cggttgccac cccaatttcg tccgtttcca gttcctcttc
     4561 ggggacaaac ttcgggacac cgaggtaggt atgcaggttg gccggattta caggaaaccc
     4621 cacacctttg ccttcggcca ctttcttcgc caccttacgc atgacgttgg cgatttcgcg
     4681 ctcaaggttc cggacgccgg cctcccgtgt atagttcgtg atgatctggc gcacggccgg
     4741 ttcggcaatg cggacatgtt tttcagtgat gccatgttcg gtaagttgtc gagggatcag
     4801 gtatttctgg gcgatgccca acttttcttc ttcggtataa ccggggattc cgatcacttc
     4861 catccgatcg cgtaaggcgg gcaaaatggg atcgatcagg ttcgctgtcg tgatgaacat
     4921 cacttcggtc aggtcgaagg gtacgcccag atagtggtcc gtgaatgagt tgttctgttc
     4981 cgggtcgagc acttcgagca atgcggcaga ggggtcgccg cgaaaatcca ttccaacctt
     5041 gtcgacttcg tccagcatga agactggatt attggtgccg gcctgcttca taccttgaat
     5101 gatgcggccg gggagcgcac cgacgtaggt gcgtcgatgg ccacggatct cggcttcatc
     5161 cctgacgccg ccgagactga tgcggacaaa ttcgcggccg agcgcacggg cgatcgactt
     5221 accgagcgag gtctttccga cgcccggggg gccaacgaaa cagagaatcg ggcctttcat
     5281 tttgtctttg agtttccgca cggcgagata ctcgagaatc cgttctttca ctttctccaa
     5341 atcgtagtgg tcctcgttga gtaccttggc ggcagccttg aggtcgagat tatccttgga
     5401 tttcttggac cagggtagtt ccacaatcca ttcgatatag gttctgacgg tcgcagactc
     5461 ggcggtatcc ggatgcattt tctcaagacg cttgagttgc ttttccgttt ctttcagcac
     5521 cttctctggc attttcagtt cggcgatccg tttgcgaaac tcggtgattt cctccgcgcg
     5581 ttcgtcgagt tcgcccagct ctttttgaat cgccttgagt tgctcgcgca ggaagtattc
     5641 gcgttgggtc ttgtccatct cccccttggc ttgcgcttga atcttttgtt gcatggagag
     5701 gacttcgact tctttgccga ggatctcgct gacatgccgg aggcgtttga tcggatcaat
     5761 gacttccagg acggcctggg tgacctcgac cttgaggccg agattggacg ctaccatgtc
     5821 ggcaagccgt ccggggtctt cgagattttc aatcacgatc atcacgtccg ggatcaagac
     5881 tttgccgaga ctcacgatcc gttcaatctg ttccttgacc gttcgcatgg cggcttcggt
     5941 ttcaagggcg gtactggccg gtttgtgttc gaggagcttg tcgatgcgca cagagtagta
     6001 gggctcagcc tggatgtagc ccgctatctt ggctttcgag agtccctgta cgaggatttt
     6061 gatgcgttca tcgggcaatt tcagcatccg catgatgatg ccgaccgtac cgacagcatg
     6121 gatgtcctcg ggtgaaggat tctcgacgtc caaggccttt tgtgtcgcga ggaagatcat
     6181 tcgattgccg g
//
LOCUS       DATIPF010000305         3428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1030534, whole genome shotgun sequence.
ACCESSION   DATIPF010000305 DATIPF010000000
VERSION     DATIPF010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030534"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VLL06_07595"
     CDS             complement(<1..548)
                     /locus_tag="VLL06_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30865.1"
                     /translation="MQAGEPVNASPAMAPQPPALSEKDLFPYKQKGRSTLTGQVFLGS
                     SSGKAITQAGAPVHLIPMTPYTRYWFDHAVRGTSCSATGNLASAETTAVPRPLTDCAQ
                     EALPQLRAEKRLAPYLRMTRANPTGHFWFTKIPAGRYYIVSLIQGGSGTHQDEEPTGL
                     AWLVLDLEAGEKVSNLVVTDCRT"
     gene            complement(626..1072)
                     /locus_tag="VLL06_07600"
     CDS             complement(626..1072)
                     /locus_tag="VLL06_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HSS30866.1"
                     /translation="MAEPSQQNLDKMWKYVKGYAEKSGTTMHPTPAVTEAVVKGLAMH
                     MDELGKPLCPCNFYKDKQEEAKLRRWMCACDEMQIYKYCHCLLFVREDGLPITEYLPD
                     GHEGREVYGVVTDPTPDKGRALKHKAPAAPADVDNTPSPASSSAAS"
     gene            1241..>3428
                     /locus_tag="VLL06_07605"
     CDS             1241..>3428
                     /locus_tag="VLL06_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30867.1"
                     /translation="MDLSDTYTRLIVRLTEELGPATAQHLVSRLFEANAKPNQMEGVL
                     LLLDELQEMSPKVARAAIESFSDLQQRDRLSDAVAWLDLGTALAESSGAVGLKFFKES
                     PLVLGLIEQASVRSFVLNTALELAEQDANIALEFLRVSPELVTVITPEQLGAWLEIGF
                     ELTHVDFVVAMEYIRQIPAVARVLPVQDARAWATFGLKLIAQNTFGKTDYFGTIEFLR
                     SSPLILGDLEDQSVRAKVVTVGSLLAERDPAAGIAWLSESPRLLLMVPNEAWRLKVLQ
                     YGALVAEHDAETALAYLRRAPELVHVIGDSAEAMARFTAWFTAGMEVLAYSVEGARAY
                     FALESQKALLSVEAALSGVPLRQVARTVKLFVQGLCGMDMTIQALPDSLSQETSARAT
                     VSRDERTISLPALLRRYPTAEENTRLYVVMAAHEAGHVEFGTYRLTLEPFADLVMVLR
                     QRYGLVKQAAPDTLASLFRLYPHPGLIQDLWTLVEDARVEFLLQQEYPGLQHDLKRFA
                     REAIATRSLTHGLTVKELVVDQLLQLSTAASQPIAIHEAIKDEIAILWPMCRAILVPT
                     ATAEESMRVAHALYVRLEELLAPKGAMIQVDRADDQSEDLGVGPSASEHYRPVTNWVY
                     RGAMNPEFIRQHDQDTGASNDEKHSVDIERMARASGGSQESSAQGQRNQEGVHRATES
                     DRLAGGRQLPSHVEELLDMKVEQPASVDDAGPGDRAVRYPEWDERIE"
BASE COUNT          667 a          904 c         1083 g          774 t
ORIGIN      
        1 gttctgcagt cggtcacgac aagatttgaa actttctcgc ccgcttcgag atcgagcacc
       61 agccaagcta gtcccgtagg ttcttcatcc tggtgcgttc ccgatccccc ttgaatcaag
      121 ctgacgatat agtagcgtcc ggcgggaatc ttggtaaacc agaaatgacc ggtggggttg
      181 gctctggtca tgcggagata gggagccaac cgtttttctg cccggagttg aggcagcgct
      241 tcttgcgcgc agtctgtcag aggtcttggt acagcagtag tttcagcgga ggcaaggttt
      301 cctgttgcgg agcaggatgt gcccctgacc gcgtgatcga accaatagcg tgtgtatggg
      361 gtcatcggga taagatggac cggggcaccg gcttgtgtga tggcttttcc tgatgatgaa
      421 ccaagaaaga cctggccggt cagagtgctt cgccctttct gcttgtaagg gaataaatct
      481 ttctcgctca gcgccggagg ctgtggcgcc atggcaggag aggcgttgac gggctcgccg
      541 gcctgcacct ggtggaacca gggacctccc aacagaagtg agatgactat tcgacatacg
      601 aattgtccgt ggagtggcat cgtcgttatg aagcggctga agacgatgcc ggtgacggcg
      661 tattgtcgac gtctgccggt gcggcagggg ctttgtgttt cagcgctcgc cctttgtccg
      721 gagtcgggtc ggtgacgaca ccgtacacct ctcgtccttc gtggccatcc ggaagatatt
      781 ctgtgatcgg caatccatct tcacggacaa ataacaggca gtggcagtat ttgtagatct
      841 gcatttcgtc gcaggcacac atccagcggc ggagcttggc ttcttcttgc ttatccttat
      901 agaagttgca ggggcagagg ggcttgccca gttcgtccat gtgcatggcg aggcctttga
      961 cgaccgcctc ggtgaccgcg ggggtaggat gcatggtcgt tccgctcttc tccgcatatc
     1021 cctttacata cttccacatt ttgtcgagat tttgttgact gggctcagcc attacatctc
     1081 cctctctgtt gtgggctcag gacgacgggt gagtggggta ggattatgac cgtgaacacc
     1141 ctgtcccaac cgattgcttc tcatcacctc gtagtttaca agcgctcggg cgtcctttac
     1201 aatccaacac ttccaaaaat tacacgggat tggatcgaga atggacctgt cggataccta
     1261 tacgaggttg atcgtcagac tgacggaaga gctgggaccg gctacagccc agcacctggt
     1321 ctctcgtctc ttcgaggcca acgcgaagcc gaatcagatg gaaggcgttt tgctgttgct
     1381 tgatgaacta caagaaatgt cgccaaaggt cgctcgtgcg gcgatcgaat cattctccga
     1441 tttacaacag cgggatcggc tcagtgacgc agtggcttgg ctggatctgg gaactgcgtt
     1501 ggccgaatcg tccggtgcgg tcgggctgaa gtttttcaag gagagccctc tggtgctggg
     1561 tttgatcgag caagcctcgg ttcggtcgtt cgtgttgaac accgccctgg aactggccga
     1621 gcaggatgcg aatatcgcgc tggagtttct ccgcgtgtca ccggaactgg tgacggtgat
     1681 cacgccggaa caactgggag cctggctgga gatcgggttc gaactgacgc acgtcgactt
     1741 tgtcgtggcc atggagtaca tccgccagat tcccgcggtc gcccgtgtct taccggttca
     1801 ggatgccaga gcctgggcga ccttcggact gaaactcatt gctcagaata ctttcggcaa
     1861 gacggactat tttggaacca ttgaattcct ccgcagtagc ccgctcatcc tgggcgatct
     1921 cgaagaccag tcggtgaggg ccaaggtcgt gacggtcggg tcgttattgg ctgagcgtga
     1981 tcccgcggca ggcatcgcct ggttgtcgga atcgcctcgc ctccttctca tggtgccgaa
     2041 tgaggcatgg cggctgaaag tcttgcagta tggggcctta gtggcggagc atgatgcgga
     2101 aacggcgctg gcgtatttgc ggcgtgcgcc ggaactcgta cacgtgatcg gcgactctgc
     2161 cgaggccatg gctcgtttca cggcctggtt cacagctggg atggaagttc tggcgtacag
     2221 tgtggaagga gccagagcct attttgctct ggagtcgcag aaggcgctgc tgtcggtgga
     2281 ggcggcctta agtggtgtgc cgcttcggca agtagcccgt accgtgaagc tcttcgtgca
     2341 agggctctgt ggaatggata tgacgattca ggcgctcccc gattcgctga gccaggagac
     2401 gtctgcgcgg gctacggtga gccgggacga gcgcacaatc tcgctgccgg cgctcctccg
     2461 tcgctatccc actgccgaag aaaacacacg attgtatgtg gtgatggccg ctcacgaagc
     2521 gggacatgtg gagtttggaa cataccggct gacccttgag ccgttcgcgg acttggtcat
     2581 ggtcctgcgt cagcggtatg gtctggtgaa acaggctgcg ccggacacgt tggcctcgct
     2641 gtttcgcctc tatcctcatc cgggactgat ccaagattta tggacgctcg tggaggatgc
     2701 acgggtggag ttcttgctac agcaggagta tcccggactt cagcatgatc tgaaacggtt
     2761 cgcgcgcgag gcgattgcga cacgctcgct gacgcacggg ctgaccgtca aagaactcgt
     2821 agtcgatcaa ttgctccaac tgtcgacggc cgcatcgcag cctatcgcaa ttcacgaagc
     2881 catcaaggac gagatcgcca tcctctggcc gatgtgccgg gcgattctgg ttccaacggc
     2941 aacggcggag gagtctatgc gggtggctca tgcgctctac gtgcggctcg aagagttgtt
     3001 ggcaccgaag ggtgcgatga tccaggtcga tcgggcggac gaccagtcag aagatctggg
     3061 tgtgggccca tccgcttccg aacactatcg ccccgtgacc aactgggtct atcgtggggc
     3121 gatgaaccct gagttcattc gtcagcatga tcaggatacc ggggcatcga acgatgaaaa
     3181 gcactcggta gacatcgaac gaatggctcg tgcctccggt gggtcacagg agtcctctgc
     3241 gcagggacag cggaatcaag agggtgtgca cagggcgact gagagtgacc ggcttgccgg
     3301 ggggcggcag ttaccctctc atgtggaaga actcctggat atgaaggtcg aacagccggc
     3361 ctcggtcgat gatgccggac cgggcgatcg tgcggtccgt tatccggaat gggatgagcg
     3421 gatagagg
//
LOCUS       DATIPF010000306         3263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1210483, whole genome shotgun sequence.
ACCESSION   DATIPF010000306 DATIPF010000000
VERSION     DATIPF010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210483"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..989
                     /locus_tag="VLL06_07610"
     CDS             273..989
                     /locus_tag="VLL06_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HSS30868.1"
                     /translation="MQAPRPVSRREFMWLSGLAAGSAGLALSTIGRAADPLVTGGDTD
                     AVLAQLFEGNKRFMKGELAHPRRKPEDFVSLAEGQAPLAVIVGCADSRVAPELIFDQG
                     VGDLFVVRVAGNVVSGTGAALKGSIEYAVAELGVRLILVMGHRQCGAVKAAIKHLDAH
                     DALPGSISGLVDIIKPAVVAVKGKPGNLLDNAIKANVERGVDRLKHLQPILAESVKKG
                     TLKVVGAVYDLHSGKVTAFA"
     gene            1304..1687
                     /gene="crcB"
                     /locus_tag="VLL06_07615"
     CDS             1304..1687
                     /gene="crcB"
                     /locus_tag="VLL06_07615"
                     /inference="COORDINATES: protein motif:HMM:NF010792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HSS30869.1"
                     /translation="MLSQIVAICLGASLGALLRWFLGMRLNFLFPTIPMGTLAANLIG
                     AYIVGLAVGYFSQAPEISPEWRLLIITGFCGGLTTFSAFSAEVMTLLQEAELGWAAAT
                     IVVHVSGSLILTFAGLFTWQFLKTT"
     gene            1697..2014
                     /locus_tag="VLL06_07620"
     CDS             1697..2014
                     /locus_tag="VLL06_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HSS30870.1"
                     /translation="MNGYQITFFTQQDRKHKGRPLGDWLVHLAKEMGLRGATLMAATE
                     GVGHDGRFHSAHFFELADQPVAVIMAVDEDESIRLLARLKAEGVHLFYVKTPVEFGIL
                     GEE"
     gene            complement(2145..2447)
                     /locus_tag="VLL06_07625"
     CDS             complement(2145..2447)
                     /locus_tag="VLL06_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30871.1"
                     /translation="MADKIPKAISNATVIKVLAKVFAFQAIEINLLRLHAGISDANWK
                     ALKTEAFKKTYAKQRSFYEDRLTGAFALERISQSERETRLLELWLKQSEDSPKDEP"
     gene            complement(2561..2908)
                     /locus_tag="VLL06_07630"
     CDS             complement(2561..2908)
                     /locus_tag="VLL06_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSS30872.1"
                     /translation="MALLITDECINCGACLPECPNEAIFETRGDAETKGNHVGDGQGV
                     GDSIYVITHDRCTECVGHFDEPQCAVVCPVDNCCISDPAYPETTDVLLDKAKKLNPDK
                     SIDPAKVWSGVRN"
     gene            complement(3050..>3263)
                     /locus_tag="VLL06_07635"
     CDS             complement(3050..>3263)
                     /locus_tag="VLL06_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30873.1"
                     /translation="DERTTFLRWVGTMDGHKLESLEVIVDNEQGLIVERTIALRPYPA
                     LKLFRDAMYASLKDKLPSDVWDYPTP"
BASE COUNT          731 a          834 c          907 g          791 t
ORIGIN      
        1 gacggttccg gtgcaccgcg acgaatagga aggggcaggc aatgggccta ggtaattgcc
       61 cctcgtaggg atattcctac gtgacgccct cagggggtga gactggggag aacccgagtc
      121 tggaaggaaa aaatcctgta ggaagacgta ggaagagatg gtatggaagt cgatttttcc
      181 tagggctttg acgtatcttt ccgccaattc caaaaaatag ttcggcatcc aagccgatcg
      241 aacctgactg ctgttcatga aggaggtatg tcatgcaagc tccgagaccg gtgtcacgcc
      301 gtgagttcat gtggctctct ggattggcgg ccggatcggc cggcctcgcc ctttcaacaa
      361 tcgggcgggc agccgaccca ctggtgacgg gaggggacac ggatgcggtg ctggcccagt
      421 tgtttgaggg aaacaaacgg ttcatgaagg gggagctagc tcacccgcgc cggaagccgg
      481 aggactttgt gtcacttgcg gaaggtcagg ccccgctcgc cgttatcgtc ggctgtgcgg
      541 actcgcgggt ggcgcccgag ctgatcttcg atcagggggt gggagaccta ttcgtggtcc
      601 gcgtggccgg gaacgtggtc tccggcacgg gcgctgcctt gaaggggagc atcgagtatg
      661 ccgtcgcgga actcggcgtg cggttgatct tggtgatggg tcatcgccag tgcggcgcgg
      721 tcaaggcagc catcaagcac cttgacgccc atgatgccct gcccggttcc atcagcggcc
      781 tggtcgatat catcaaaccg gccgtggtcg cagtgaaggg caaacccggt aacttgctcg
      841 acaacgcgat caaagctaac gtcgagcgag gcgtggatcg gctcaagcac ttacagccca
      901 tactggccga atcggtcaag aaggggacgc tgaaggtcgt cggtgccgtg tacgacttac
      961 acagtggcaa ggtcacggcg ttcgcgtgag ggggagtgag gcacactatc ttggccccaa
     1021 gtggactctc aggactaacg tgatacacag cagtatcacc tactcaagga ggcggcagga
     1081 tctaaatcgt cattgactca gaaacagttg gttttgatat cagcgaggtc tcgggaaaac
     1141 tccttttgtt ttcaggttgc gtgttaggta tcttgggaga ggattttgaa ccgggccatg
     1201 tggtgcattc aaagagggtg acagatgtat gaggcgatgc taacgccctg atcggtaggc
     1261 agatcacgca ccgtcccaaa ccgttcggag attgattgga tttatgttga gccagattgt
     1321 tgccatttgt ctcggcgctt ctttgggagc attgttacgc tggttcctcg gaatgagatt
     1381 aaactttctc tttccgacca tcccaatggg aacacttgca gccaacctca ttggggcata
     1441 catcgtaggt cttgcggtgg gctacttttc gcaagcgcct gaaatatcac ccgaatggag
     1501 actcctgatc attactggtt tctgtggcgg cttaacgaca ttttctgcgt tttcagcgga
     1561 agttatgact ctgctccaag aagccgaact aggttgggct gccgcaacaa tcgttgtcca
     1621 cgtgagcggc tcactcatct tgacctttgc aggacttttc acctggcagt tcttaaagac
     1681 tacatgaatc atgcgtatga acggctatca aattacattt tttacgcaac aagatcgcaa
     1741 acacaaaggg agaccattgg gggactggct cgttcatcta gcaaaggaaa tggggttacg
     1801 gggagcaact ctaatggcag caaccgaggg agtgggacat gatggacggt tccactcagc
     1861 tcatttcttc gaactcgccg atcaaccagt cgccgtgatc atggctgtgg atgaggatga
     1921 atctatccgc ctacttgctc gactcaaggc ggaaggtgtt catcttttct atgtcaaaac
     1981 acctgtcgag tttggaattt taggcgagga gtaaatcgag ttgattggat gccgttgtca
     2041 cggttctttc ccaggtctgt attcttgcaa acccccagtt tgaggttccc ggatctgccc
     2101 gatctgcagt ttagccgaaa cgagctgcag ttgagagcac tggttcaggg ttcgtctttg
     2161 ggagaatcct cactctgttt tagccagagt tcgagcagtc ttgtctcgcg ttcagactgg
     2221 gaaatgcgtt ctaaagcgaa cgcacccgtc aagcgatctt cataaaaaga gcgttgcttc
     2281 gcgtaggttt tcttaaaggc ttcggtcttg agggctttcc aattggcatc tgatattcca
     2341 gcatgtaaac gcaataggtt gatctcgatg gcttggaaag caaagacttt agctaacacc
     2401 ttaatgacag ttgcattaga aattgctttt ggaattttat cagccacgac tgatcgtttc
     2461 ggttatgccg aggcaaacaa gttaatcttc tcttcgtacc tcatccgcga aggcggcgtt
     2521 tttagcattc tacacgtggc gcggtacttc gaagtcacga tcagttccgc acgccgctcc
     2581 acacttttgc tggatcgata gacttgtcag gattgagttt cttcgccttg tccagcaaga
     2641 catccgtggt ttccggatag gcagggtcag aaatgcagca attgtcgacc ggacagacca
     2701 ccgcgcattg aggttcgtca aaatgtccga cacactcggt acagcggtca tgcgtaatca
     2761 catatatact gtctcccact ccctggccat cacccacatg atttcccttc gtttctgcgt
     2821 cgccgcgagt ttcgaagatg gcctcgttcg gacattctgg cagacaggct ccacagttga
     2881 tacattcgtc ggtaatcagc agcgccatta cactccctcc ttcttctcga ccaaaatagg
     2941 tgaatacctt atctagcacc caaaggcacc ggttggtcaa gtgctaactc acagctgacc
     3001 aactagattc ctgggtcgac ccgatctgaa acgccccgtc gtggtcacac tacggcgttg
     3061 ggtagtccca cacatcggac ggcagcttat ccttgagcga tgcatacatg gcgtcgcgga
     3121 atagtttcag agccggatac ggccggaggg caatagtgcg ctcgacgatt agcccctgct
     3181 cgttgtccac gatgacctcc aagctctcca acttatggcc atccatagtc cccacccagc
     3241 gcaaaaaggt ggtgcgctca tcg
//
LOCUS       DATIPF010000307         6398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_147995, whole genome shotgun sequence.
ACCESSION   DATIPF010000307 DATIPF010000000
VERSION     DATIPF010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147995"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..565)
                     /locus_tag="VLL06_07640"
     CDS             complement(44..565)
                     /locus_tag="VLL06_07640"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSS30874.1"
                     /translation="MTMQFQKIDPRVTITTPLGEIKIRFYPDDAPRHVENFIKLIKMG
                     FYNGTTFHRVVPGFIIQGGDPLSKLPDRSLHGTGSPGYWLNPETSDRPHKRGAIGMAK
                     VPRESNSTRDPNDNGSQFYICVDDNSGLDRTYTVFGEVFRGMDVVDKIVAAARDEADN
                     PLQPITITMTVKE"
     gene            complement(626..1909)
                     /locus_tag="VLL06_07645"
     CDS             complement(626..1909)
                     /locus_tag="VLL06_07645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSS30875.1"
                     /translation="MNTLTPRPSRIRWLILALLFLISVVTYIDRVNISVTARQMMPAY
                     GITDQQMGWVFSAFVVGYALFQIPGGWLADHWGVRAVLTVALLWWSICTAFTAIAAVS
                     PLVSLVGIVGALMVVRFCLGIGEAVALPAFNRAVTNWFPADARGFGIGIAIGGIGLGS
                     AMTPPIAAWVMVNWGWQTVFYLASLIGIVMGLLWWLLSRNHPHEHPWVHAGRQTPLTS
                     EPTTATAVPWRTFRRTPTIWWLVISYSCLGYVAYVYLSWFYLYLVNVREFGVLRGGMY
                     ASIPFLAMLVFCPLGGWVTDRLAARYGLTTGRVVAGMTGMFLAGTAIALGGVVESPYL
                     AIASLSLGAGWLYFTVGAYWASTTDLSKAHAGTLSGLMNTGANIGGAISPTLTPWLAH
                     QWGWPVSLGFAAVVAILGGVLWFKIDPGKGLNRES"
     gene            2283..2840
                     /locus_tag="VLL06_07650"
     CDS             2283..2840
                     /locus_tag="VLL06_07650"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HSS30876.1"
                     /translation="MASAFSHAVVALAMGKAVRNKELGWRELSLGALCSIVPDLDVLG
                     FPLGIEYGDMWGHRGITHSVVFAALLAGILVAVWYRTKPAIALTGLFIYFFLCTASHG
                     VLDALTDGGLGVAFFSPFDTTRYFFPMRPVLVSPIGMREFFTEYGARVLANEFLWIWL
                     PSCAVFMMLRAIQWLWPVKSVAPLS"
     gene            complement(2911..4620)
                     /locus_tag="VLL06_07655"
     CDS             complement(2911..4620)
                     /locus_tag="VLL06_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS30877.1"
                     /translation="MLLGLPFAAVLTIVPYSFAEDPPVHPEEQVAERAKGAWESGAIN
                     PALDILGQGIHDHPDALMLQKLRGDILATFRDPQEAVQAYQTVLAKMPAALDVRWAKW
                     SVLVRSGQGAEAIAELRRMALIDVQNPLIHLRLAQELRKLDRLEESLDSYKKAVDLVP
                     DLIGWRLALARARFDVLDYQGAEDDVQDVLHKMPIGSPLELPAKNLLSQINGTSIDRG
                     RRFDPVHTTDMTGAQRKEWAAIRAEAWKLFSTGHYQEAEPIYQRMLALNPNDALANYQ
                     LGLTLMQLGRCKDALTVFGKLSNLDPSDEDYADTVYRMGQCLTELEQWEDAFVHFQTL
                     YDAAVEFEKNNKDVQLPPDARVLDKKKIARWLDKVRPHVPELAQMADDEASNPAPSDD
                     LSPVEISPEEALYQKASERFKPQKPLDPRASLMGRDADFSWFRFVIPAGKVMRDDFPS
                     GAHEFIPLNPGDSFPATQQEIYLVFELVSASFDAIPLTGRCVPETSEMSGETPAVTQD
                     RVMTTMNDQSGYFMLTPPKVGWAPGLYHCGLYTGELTSADTLVDEVRFRIVGPARLST
                     QGS"
     gene            4814..5296
                     /gene="trmL"
                     /locus_tag="VLL06_07660"
     CDS             4814..5296
                     /gene="trmL"
                     /locus_tag="VLL06_07660"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HSS30878.1"
                     /translation="MLDYSPMFDVILYQPEIPPNTGNIIRLCANSGARLHLVKPLGFT
                     LEDKQLLRAGLDYHEFATITVYESWAECAECFKDRRLFAVSTKGTQRYDLVAYADGDV
                     FVFGPESRGLPSEILGTVDKQRCIRVPMVPESRSLNLSNAVAVVIYEAWRQIGFGTCR
                     "
BASE COUNT         1359 a         1723 c         1893 g         1423 t
ORIGIN      
        1 cgcgctccgg gagcaggggg tcagcgcggg tgtgactcca cgcttattct ttgaccgtca
       61 ttgttatcgt gatcggctgc agaggattgt cggcttcgtc gcgggcggcg gcgacgatct
      121 tgtcgacgac gtccattccc cggaagactt cgccgaagac ggtgtaggtt ctgtccagcc
      181 cgctgttgtc gtcgacacag atgtagaact gcgagccgtt atcgttgggg tcccgtgtgc
      241 tgttgctttc ccgtggcacc ttcgccatgc cgattgcgcc ccgtttatgc ggacgatcgc
      301 tggtttcggg attcagccag tagccgggac tgccggtccc gtgcagcgag cggtcgggaa
      361 gcttactcag cggatcgccg ccctgaatga tgaatccggg aacgacacga tggaaggtgg
      421 ttccattata gaatcccatc ttgatgagct tgataaagtt ctcgacgtga cgcggcgcgt
      481 cgtccgggta gaagcgaatc ttgatctcgc cgagcggtgt ggtgatggtg acgcgcggat
      541 cgatcttttg aaattgcatg gtcatgggcc gaacgtacca ggggcgagag gtcggaggca
      601 atgggttcga acgtaagggg gaagatcagg attcacggtt caaccccttg cccgggtcaa
      661 ttttgaacca taggactcca cccaagatgg ccacgactgc ggcaaatcct agcgagaccg
      721 gccagcccca ctgatgggcc agccacggcg tcagggtggg ggaaatggca ccgccgatat
      781 tggcgcctgt gttcatgaga cctgataacg tccctgcatg ggctttggac agatcagtcg
      841 tcgaggccca gtaggcgccg acggtaaagt agagccagcc cgcaccgagt gacaaactgg
      901 cgatcgccag atagggggat tcgacgacgc cacccagcgc aatggcggtc ccggccagga
      961 acatgccggt catgccggct acaacccgtc cggtggtgag accgtagcgc gctgcgagtc
     1021 gatcggttac ccagccgccc aggggacaaa acaccaacat agccagaaag ggaatggacg
     1081 cgtacattcc gcctctcagc acaccgaatt ctcgaacatt gacaagatac aggtagaacc
     1141 aggacagata gacgtaggcg acatagccga ggcaactgta gctgatgacc aaccaccaaa
     1201 tagtgggggt ccgtctgaat gttcgccaag gcacggcggt ggcggtagtt ggctcgctcg
     1261 tgagcggagt ctgtctacct gcatggaccc atggatgttc gtgcggatga ttgcgagaca
     1321 ggagccacca gaggagcccc atcacgattc cgataaggct cgccagatag aacacggtct
     1381 gccatcccca gttcaccatc acccatgcgg caatgggtgg ggtcatggcg gagcctaatc
     1441 cgatgcctcc gatcgcgatc ccgatgccga agcctcgcgc gtcagccggg aaccagttgg
     1501 tgacggcgcg attgaaggcc ggtagggcta cagcctctcc gatccccaag cagaaccgca
     1561 ccaccatcag cgccccgaca atcccgacga ggctcacgag cggcgacaca gccgcgatgg
     1621 cggtgaaggc cgtacagatc gaccaccaga ggagtgcgac cgtaagcacc gctcggactc
     1681 cccagtgatc ggccagccat ccaccgggaa tctgaaacag ggcgtaaccg accacgaagg
     1741 ccgagaagac ccagcccatc tgttgatcgg ttatcccata ggctggcatc atctgacgtg
     1801 cggtcacgga aatattcacg cgatcgatat aggttacgac gctgatgaga aagagtaagg
     1861 cgaggatgag ccagcgtatc cgtgaggggc gaggcgtcag tgtgttcaca aggttgagtc
     1921 gtggggtctg ttcatcggag tgccggtgcc ggattataca gactccgggc gtacgtcact
     1981 accggtacga ggagtggagg tggaggggag gggtagcctg atcggtctcc tttgctcgcg
     2041 caacgcgcgc cctaggaagg gcctcgttgg acgcgcgcag tgggagaccg attcagggca
     2101 ccctccttgg agaggataca agcgagcttg gagagacgat tagagtgctc gttcgaggcg
     2161 cgcaattgag gatcaatcag gctgccctgt gaggagtata actggacacg cacagtattc
     2221 ttgacgtgcc tgttacccgg gggtagggtc tcatggtggc tggatcgtag tagaggtatt
     2281 gaatggcgtc ggcattttct catgctgttg tcgcgctggc gatggggaag gccgttcgga
     2341 acaaggaact gggctggcgt gagctgtcct tgggggcact ctgttctatt gtcccggatc
     2401 tggatgtgct ggggtttccc ttgggcattg agtacggcga catgtggggc catcgtggga
     2461 taacccactc ggttgtgttc gcggcgctgt tagccgggat tcttgtggct gtctggtatc
     2521 gaaccaaacc ggcgatcgcg ttgacgggac tctttatcta tttcttcctc tgcaccgctt
     2581 cgcatggcgt gcttgatgca ttgacggatg gagggttggg cgtcgcgttt ttctctccat
     2641 tcgacacgac aagatatttc ttccccatgc gaccggtctt ggtgtctccc atcgggatga
     2701 gggaattctt caccgagtac ggtgcccgtg tcctggccaa cgaattcctg tggatttggc
     2761 tgccctcctg cgcggtgttc atgatgcttc gggcaatcca gtggctctgg ccggtcaagt
     2821 cggtggcacc tctctcatag ccgttgttac tcgctcgcaa ccaacgcgat cagttctcag
     2881 ccttcagctt ccatcgaaaa tagatacgtt tcacgagccc tgcgttgata atctggctgg
     2941 cccgacaata cgaaatctga cttcatccac aagggtatct gcggacgtca gttcgcctgt
     3001 gtataaccca cagtggtaga gaccgggtgc ccatccgact ttgggaggag tgagcatgaa
     3061 atagccggac tggtcattca tcgtcgtcat gacccggtcc tgtgtgacgg cgggtgtctc
     3121 tccactcatc tcggatgttt ccggcacaca gcgtcctgtg agagggattg catcgaatga
     3181 ggccgatacc agttcaaaga ccaagtagat ttcttgttgc gtggcaggaa aactatctcc
     3241 agggttcaac ggaataaatt catgggcgcc ggatggaaaa tcatcccgca tgaccttacc
     3301 cgccgggatg acaaagcgga accaactgaa atcggcgtct ctgcccatga gcgacgcccg
     3361 cggatccaga ggtttttgcg gtttaaaccg ctcggaggct ttctgataca gcgcttcctc
     3421 cggagaaatc tcaaccgggg ataggtcgtc ggaaggggct gggttgcttg cctcatcgtc
     3481 cgccatctgt gccagttcgg gaacatgtgg ccgcaccttg tcgagccatc gtgcgatctt
     3541 ctttttatct aacacgcgag catccggtgg aagctgaaca tccttattgt tcttttcgaa
     3601 ctcaacggcc gcgtcataga gggtttgaaa atggacgaac gcgtcttccc actgttccag
     3661 ctccgtgaga cattgaccca tccgataaac cgtatccgca tagtcttcat cgctgggatc
     3721 cagattggat aattttccaa atacggtcag tgcatccttg caccggccaa gctgcatgag
     3781 ggtcaaccct aattgatagt tggcgagggc atcgttggga ttgagtgcca gcattctttg
     3841 atagatcggt tcagcttcct gatagtggcc tgtcgagaaa agcttccagg cttctgcgcg
     3901 aatggcggcc cattccttgc gctgagcccc ggtcatgtct gttgtatgaa cggggtcgaa
     3961 gcgccggcct cgatcgatag aggtgccatt gatctgtgag agcaaatttt tggccggaag
     4021 ctccagtgga gagccgatcg gcatcttgtg caacacgtct tgcacgtcgt cttccgcacc
     4081 ttgataatcc agcacgtcaa atcgcgcccg ggccaacgcg agccgccagc cgatcaaatc
     4141 cggaaccaga tccacggctt tcttatacga gtcgagcgat tcctcaaggc gatcgagttt
     4201 tcgtagttct tgcgccagcc gtaaatggat caagggattc tggacatcga tcagcgccat
     4261 gcgccgtaat tccgcaatgg cttcggctcc ctgccccgat cgaaccaaca cactccactt
     4321 ggcccatcga acatccaacg cggccggcat cttggcgagg acagtctgat aggcctgaac
     4381 cgcttcttgt gggtctcgga acgtggcaag aatatcgccg cgtagtttct gcagcatgag
     4441 cgcgtccgga tggtcgtgga tcccttggcc aagtatatcg agtgcagggt tgatcgcgcc
     4501 gctctcccac gcccctttcg cacgttcggc gacttgctcc tccggatgta ctggtggatc
     4561 ttccgcgaac gaatagggaa cgatcgtcag cactgcagca aacggcaggc caaggagcat
     4621 ggagatggag tgatatcttg gccggatggg agcgtcttgc ctgtgaggtg gcagcgatct
     4681 atgcatgacc ctgtgggatt cccttcatag agattcacta taccaagaag ccatgtgagg
     4741 gagaaataaa ccagggtttc tttgtccgtc tctcagggtt cgtttctcct gcatcgagga
     4801 acgcgacatc gccgtgctag actactcgcc catgttcgac gtcatcctct atcagcctga
     4861 gattcctccg aacaccggta acattatacg actctgtgcc aatagcggag ccaggctgca
     4921 tctcgtgaaa cccctgggat ttacgctgga ggataagcaa ttgctgcgag cagggctcga
     4981 ctatcacgaa ttcgccacga ttacggttta cgaatcctgg gccgaatgtg ccgaatgttt
     5041 caaggatcgc cgtctgtttg cagtgtccac caaaggcacc caacggtatg acctggtcgc
     5101 ctatgccgac ggcgatgttt ttgtgtttgg cccggaaagc cgtgggttgc cgtctgagat
     5161 cttggggacg gttgataagc agcgttgtat ccgcgtaccc atggtcccgg agagccgcag
     5221 cctcaatctc tccaacgccg tggcggtggt catctacgaa gcctggcggc agatcggatt
     5281 tgggacctgc cggtaagtcc gagaaaggat gaagctggtt tgttctgtcc tggtagcagc
     5341 gtagtagcgg aggggacgag atttgaaaac cctccagcgc cagagactat agggcctgat
     5401 ctcaccgtat cggttcaatc gcaatcactt acagaagagt cggaggcatg cggggaaccc
     5461 attaaccccg attggccacg atagctctgc cacagttgag ccacagggat gttacgagcg
     5521 gaacggacga agttggttta cttcgattta tggcacctga cgaggagcag gaagttcaaa
     5581 gaaacaacga gtgcccgcca ggtacatctt gactcggtca cttactggaa tgggtcgcac
     5641 ccaacatact tccaccaatt tggtgcgcgg cgttttcctc gcctctgagg gcacctgaat
     5701 ttcaacaacc tgccgtctgt ttagtggcac aggaagtaag agtagcaggc ccctgacgct
     5761 cttattgatc gcatgcgcga atccctttgt cagcttcaca gtgttcttgt ccacaaattt
     5821 cgtcagtttg aaggaacaaa cgagcctgca gggcgcacgc atgctatctc tttgctctcg
     5881 tgcaacctga atcatctggc tgacgcgtac tagttgaccg ctcggtttac ccatagcatg
     5941 ttccctctcc ggcaggatgc ctttgtaagc catatgtgaa ggcctgcgcc agcgatttcg
     6001 gttgccaggc gcaaggttcg caatggaatc tgtgcaattc agtagaatct agccgagcaa
     6061 tgagggggga tcactctgcg aggggcttgc aacgtagcga tgctgcttgt ggatgcggat
     6121 gttagcactt cgaggtgaat tcaattccgg acctccaagt gtgaagtaga atatatactg
     6181 acgccgctgc acgcgctgcg tcaaggggta cattttcccc atcgcttcaa aactggaaac
     6241 gctagacaag atgcctggct cgcaccacac tggtgcggtg cgacatatga ctctatgggc
     6301 aagaaacggc ctagaaagtc atcgtgagtt gggagattat cgatgaagaa cgatcgctgg
     6361 tgttacatcg ttgcggcggc gtcgggagta cttggatg
//
LOCUS       DATIPF010000308         7324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1653385, whole genome shotgun sequence.
ACCESSION   DATIPF010000308 DATIPF010000000
VERSION     DATIPF010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7324
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653385"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1877)
                     /locus_tag="VLL06_07665"
     CDS             complement(138..1877)
                     /locus_tag="VLL06_07665"
                     /inference="COORDINATES: protein motif:HMM:NF006903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006847 - plasma membrane acetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HSS30879.1"
                     /translation="MIGLHRTYPLAARCGLTVVALCTVATSVYAAGPDVGQAQQQAIN
                     WTAIGMFGVFVLLTLFITKWAAARTKSAAVFYTAGGSITGFQNGLAIAGDFMSAASFL
                     GISAAVMASGYDGLIYSIGFLVGWPVLTFLMAERLRNLGKFTFADVAAFRFKQAPIRI
                     FAASGTLVVVAFYLIAQMVGAGQLIRLLFGLEYWMAVVIVGALMMVYVLFGGMTATTW
                     VQIIKACMLLGGATFMAFMVLLNFGFSLEAMFANAVEIKAGLATAAGKTSAEAAAAGQ
                     SIMGPGNFVKDPISAISFGMALMFGTAGLPHILMRFFTVPSAKEARTSVMWATGWIGY
                     FYLLTFIIGFGAITFVLTNPEFLDANGALKGGSNMAAVHLASAVGGNVFLGFISAVAF
                     ATILAVVAGLTLSGASAVSHDLYATVVKGGKPDGASELRVSRMTTVALGILAVLLGIV
                     FEKQNVAFMVSLAFAIAASANFPVLFMSVLWKDCTTRGAVIGGFLGLISSVLLTVVSP
                     SVWEATLGYPKGSALFPYASPALFSITIGFVGIWLFSILDRSSQAAKDRDGFLAQQVL
                     SETGIGATTASGH"
     gene            complement(1874..2182)
                     /locus_tag="VLL06_07670"
     CDS             complement(1874..2182)
                     /locus_tag="VLL06_07670"
                     /inference="COORDINATES: protein motif:HMM:NF016244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HSS30880.1"
                     /translation="MDTELVTRIANNPNYQALKARRTRFGCWLTLAMMIAYYGFILLV
                     AFNKSFLSQKLGVGVMTVGIPIGFGVIVFTIVITAYYVNRANREFDALSDAIAKEVLK
                     "
     gene            2181..2360
                     /locus_tag="VLL06_07675"
     CDS             2181..2360
                     /locus_tag="VLL06_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30881.1"
                     /translation="MVLPKRHGFDANDHAYLARIHDPYVRPNAQRRARTDLHPDFQWA
                     QSLDQQRGTHDGIVT"
     gene            complement(2611..5121)
                     /gene="uvrA"
                     /locus_tag="VLL06_07680"
     CDS             complement(2611..5121)
                     /gene="uvrA"
                     /locus_tag="VLL06_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HSS30882.1"
                     /translation="MHNSIVIKGAREHNLKNLDVTIPRDRLVVITGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLEQMGKPDVDSIEGLSPAISIEQKSTSHNPRSTVGTV
                     TEIYDYLRLLFARVGRPSCFQCGEEITAQTVQQMVDAIGSLPENSKFQLLAPIVRGRK
                     GEYRKELLDMRRAGYVRARIDGKNIDLGDDISLDKQKKHMIEIVVDRLVMKSGDALMR
                     RLADSVETSLKLSGGLVGVLTDNGHVSLYSEKLACIRCGISYPEITPRVFSFNSPHGA
                     CPACDGIGYDMTPGSPDDEDFTLLEPCDVCKGARLRPESLSVKVATQSIAQVTHLSVR
                     AAADFFSSVKFSERELVIAHRILKEIRERLGFLVNVGLDYLTLDRAAATLSGGEGQRI
                     RLATQIGSGLVGVLYILDEPSIGLHQRDNRRLLQTLLRLRDLGNTVVVVEHDAETMMA
                     ADHLLDMGPGAGTHGGQVIAQGTPMDVMGNLDSLTGQYLRGTQMVSLPRRQRKAKGFL
                     SIVGATKHNLKGMTAKIPLGLLTCVTGVSGSGKSTLVLEVLFHSLSQLLYHKKPKIDG
                     CKELLGADALDKVIDIDQSPIGRTPRSNPATYTGLFGFIRDLYSNLPESRVRGYKPGR
                     YSFNVKGGRCEACQGDGLIKIEMHFLPDVYVTCEVCKGQRYNRETMEIHHKGKSIADV
                     LNMTVDDAVEFFEHIPFIKRKLDTLHDVGLHYVKLGQSATTLSGGEAQRVKLSRELSK
                     RPTGRTMYILDEPTTGLHFADVQRLLDVLDRLVEAGNTDVVIEHNLDVIKNADWVIDL
                     GPEGGDRGGEIVAEGPPKEIAKSKRSYTGQVLKEAGVG"
     gene            5157..5975
                     /locus_tag="VLL06_07685"
     CDS             5157..5975
                     /locus_tag="VLL06_07685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSS30883.1"
                     /translation="MTNHKGRCYHALMVHVDIPPVQKGTDSPAAPVAVSTWSGQAREQ
                     AVQRMFTSIAGVYDLNNTLLSFGLHYRWKTITASYVPVTTGGRALDVGSGTADLALLI
                     EPRMGTGGRVIASDLNHAMLVEGLKKVTARGLGERITCLEANAEHLGFPDRTFHAVTT
                     GFCMRNVGDLRQALSDIHRIVQPGGRFVCLEFSRPVFGWLRALYDWYSFRLLPWIGTK
                     VAHDTTGVYEYLPASIRNFPDQERLKQMLLDAGFRQVTYRNLSGGIVAIHVATK"
     gene            6071..>7324
                     /locus_tag="VLL06_07690"
     CDS             6071..>7324
                     /locus_tag="VLL06_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSS30884.1"
                     /translation="MESILYIFLPCKKVYPIGVTYLADFIHRRKPEVRQRIVDLSLFP
                     DAQRIKAVRDAATEFKPDLVCFSWRDIQIFSPHEGDSSLEHAFNFYFASNPLKRVVAS
                     FAGLQQLYRYYSHIRAALSYPWLIAKEFPKAQIMIGGGAFTAFADQLILKLPEGTIGI
                     LGEGEDAILKVIEGQSLEKERYIIREGKTIRKGQQGSPALLDALTVDLPYLTSIFPQY
                     QEYLGESIGVQSKRGCPYDCAFCLYPYIEGKRVRYRPPAMVVKDISQHYHQWGARRFW
                     FTDAQFITGKEAYPQCIEILERILAEKLEIEWSGYIRTSLITPELAKLMVRSGVGDLE
                     VAITSGSQVVLNDLHMGFKLERLYDGCRYLAEAGFKGKVILNYSLNSPKETEESLLQS
                     VVVDVTVKRMGVARALMSVMYISFTS"
BASE COUNT         1565 a         2180 c         2079 g         1500 t
ORIGIN      
        1 gggtccgatg ggatttgccg gcacttgaac acgatactcg acgaaggcaa tgtccagtgt
       61 acgaccgctt cgacaagcca atcgagcacg tcgtccacca ttcgcctgtc gccgtgcccg
      121 gttcacagga gcccggttca gtgaccggat gcggtcgtgg cgccgatgcc cgtctccgac
      181 agtacctgtt gagccagaaa gccgtcacgg tcctttgccg cctggctgct tcggtcaagg
      241 atcgagaaca accagatgcc cacgaatccg atcgtgattg aaaacagcgc cggcgaggcg
      301 tacgggaaca acgcgctgcc tttcggatag cccaaagtgg cttcccacac cgagggcgaa
      361 acgaccgtga gtagcaccga tgaaatcagc ccgagaaaac cgccgatgac cgcgccgcgg
      421 gtcgtgcagt ctttccacaa cactgacatg aacaacaccg ggaagttggc cgatgccgcg
      481 atcgcgaacg ccagggacac catgaacgcc acgttctgtt tctcgaacac gatacccagc
      541 aacacggcca ggattcccag cgccaccgta gtcatgcgcg acacccgcaa ctcggaagcg
      601 ccgtcgggct tgccaccctt gacgaccgtt gcgtacaggt catgtgacac ggctgacgca
      661 cccgacagcg tgagaccggc aacgaccgcc aggatggtgg cgaatgcaac ggccgagatg
      721 aagccgagaa acacattgcc acccaccgcg ctggccagat gcaccgccgc catgttgctg
      781 cctcccttga gcgcaccgtt ggcatcgagg aactccgggt tagtcagcac gaaggtgatg
      841 gcgccgaaac cgatgatgaa ggtcagcagg tagaaatagc cgatccatcc ggtcgcccac
      901 atcaccgacg tgcgcgcttc cttggcgctc ggtaccgtga aaaaacgcat caggatgtgc
      961 ggcaggccgg ccgtgccgaa catcagcgcc atgccgaacg agatcgccga gatcgggtcc
     1021 ttgacgaagt tgcccggtcc cataatcgac tgaccagcag ccgctgcttc cgccgaggtc
     1081 ttgcccgcgg ctgtggccag gccggctttg atctcgaccg cgttggcaaa catcgcctcc
     1141 aggctgaagc cgaaattcag cagcaccatg aacgccatga aggtggcgcc gcccagtagc
     1201 atgcaggcct tgatgatctg cacccaggtg gttgccgtca tgccgccgaa caacacatag
     1261 accatcatca gcgcgccgac gatgaccacc gccatccagt attcgagacc gaacaggagc
     1321 ctgatcaact ggccggcgcc aaccatctgc gcgatcaggt agaacgccac caccaccagc
     1381 gtgccagagg ccgcgaagat gcggatcggc gcctgcttga accggaacgc ggcgacatcg
     1441 gcgaaggtga acttgccgag gttgcgcagg cgctcggcca tcaggaacgt gagtaccggc
     1501 cagccgacca aaaagccgat cgagtagatg aggccgtcgt agccgctggc catcaccgct
     1561 gctgaaatac ccaggaacga tgccgcagac atgaagtcac cggcaattgc cagaccgttc
     1621 tggaaacccg taatgctccc acccgctgta taaaaaacag cggccgactt ggttcgagcc
     1681 gccgcccatt tcgtgatgaa cagcgtgagc aatacaaaca caccgaacat gccgatcgcc
     1741 gtccaattga tcgcctgctg ctgtgcctgg ccgacatccg ggccagcggc atacaccgat
     1801 gtcgcaacag tacagagcgc aaccacggtc agtccgcaac gtgctgcaag gggatacgtg
     1861 cgatgcaagc cgatcatttc agcacctctt tggcaatggc gtcggacaac gcatcgaact
     1921 cgcgattggc gcgatttacg tagtacgcgg tgatcacgat cgtgaacaca atgacgccga
     1981 agccgatcgg aatgcccacg gtcatcacgc cgacgccaag tttctgtgac agaaagctct
     2041 tgttgaacgc caccagcaga atgaagccgt agtaggcgat catcatcgcc agggtcagcc
     2101 agcagccgaa gcgggttcgc cgggctttca gcgcctgata gttcggattg ttcgcaattc
     2161 gcgtgaccaa ctcagtatcc atggtgcttc ctaaacgtca tgggttcgat gctaacgacc
     2221 atgcgtacct cgctcggata cacgatcctt acgtgcgacc aaatgcccaa cgccgtgccc
     2281 gcactgacct gcatccggac ttccagtggg cgcaatcgct ggatcagcag cgaggcacgc
     2341 acgacggcat tgtcacgtga acgtagctgt gttgcaataa accgacgctt tctaaccgcc
     2401 gctacctccg ccacggttcc tcatcacgag tgtgtcggta ccgtgggttg gttggaggta
     2461 cctggcacct gggtgaggtg tatctgaaca tcaacggtca gttcaaattc cgatggtgtg
     2521 gcgttgatca agaagggcag gtcatcgata ttctggcgca gaaccggaga gattccattg
     2581 cggccgagcg atcccgtcga aggcttgtat ttatcctacc cctgcctctt ttagcacctg
     2641 tcccgtatac gaccgcttcg acttcgcaat ctcttttggc ggcccctctg cgacaatctc
     2701 accgccgcga tcgccaccct ccggcccgag gtcgatgacc caatccgcat tcttgatgac
     2761 atcgagattg tgctcgatca cgacgtcggt gttgccggct tcgacaagcc gatccaacac
     2821 gtccagcagc cgctgtacat cggcgaagtg aagcccggtc gtcggttcat ccaaaatgta
     2881 catggtgcgt ccggtcggac gttttgacag ttcgcgcgag agcttgactc gttgcgcctc
     2941 gcctccggac agcgtggtgg cagattggcc aagcttcacg tagtgcaatc cgacatcgtg
     3001 caaggtgtcg agcttccgct tgatgaaggg gatatgctcg aagaactcca cggcatcatc
     3061 caccgtcata ttcagcacgt cggcgatgct cttccccttg tggtgaatct ccatcgtctc
     3121 gcggttgtac cgttggccct tgcagacctc gcaggtcaca tacacatccg gaaggaaatg
     3181 catctcgatc ttgatcaacc catcgccctg gcaggcctca cagcgtccgc ctttcacatt
     3241 gaagctatag cggccgggct tgtacccccg cacgcgggac tcaggtagat tggagtagag
     3301 atcgcgaata aaaccgaaca gacccgtata ggttgccgga ttcgagcgcg gagtccggcc
     3361 gatcggggac tgatcgatgt cgatcacttt atccagcgca tcggcaccca acagctcctt
     3421 gcagccgtcg atcttgggtt tcttgtgata gagcaactgg gagagggaat ggaacagcac
     3481 ctcgaggacc agcgtacttt ttccggatcc cgacacgccg gttacacagg tgagcagccc
     3541 gagcggaatc ttggcagtca tccccttgag gttgtgtttc gtggccccca cgatggacaa
     3601 gaaccccttg gccttacgtt ggcggcgagg caggctgacc atctgcgtgc cacggagata
     3661 ctggcccgtc agtgagtcga gattccccat gacgtccatc ggcgtgcctt gggcaatgac
     3721 ctggccccca tgcgtaccgg cgccaggccc catatccagt agatggtccg ccgccatcat
     3781 ggtttctgca tcgtgctcga ccacgaccac tgtgttgccc aggtcacgca ggcgcagcag
     3841 ggtttggagc aagcgccgat tatcgcgctg atggagaccg atcgagggct catcgaggat
     3901 gtagagaacg ccgacgaggc cggaaccgat ttgcgtggcg agcctgatgc gctggccctc
     3961 tccaccggat agggtagcgg ctgcccgatc caatgtcagg taatccaagc cgacgttcac
     4021 cagaaacccg agccgttcgc ggatttcttt caagatgcga tgcgcaatca ccagttcgcg
     4081 ctcgctaaat ttcacagaac taaaaaaatc agcagccgct cgtacggaga gatgtgtcac
     4141 ctgcgcaatc gactgggtgg cgaccttgac ggacaggctt tcaggccgca acctcgcgcc
     4201 tttgcagaca tcgcagggtt ccagcaatgt gaagtcttca tcatctggag agccgggggt
     4261 catgtcgtaa ccgatgccgt cacaggctgg gcaggcccca tgtgggctat tgaacgaaaa
     4321 gacacgcggt gtaatttcag gataactgat gccgcagcgg atgcaggcga gcttctcgct
     4381 atagagtgag acgtggccat tgtccgtcaa cacaccgacc agtcctccgg agagcttcaa
     4441 ggaggtctca accgaatcgg ccaaccgccg catcagcgca tcaccagact tcatcaccag
     4501 ccgatcgacc acgatctcga tcatgtgttt cttctgtttg tcgagcgaga tgtcgtcgcc
     4561 caggtcgatg ttcttgccgt cgatgcgggc gcggacatac ccggcccggc gcatgtcgag
     4621 gagttctttt cgatactctc ccttcctgcc tcggacgatt ggagccaaga gctggaactt
     4681 gctgttctcg gggagtgagc cgatggcatc gaccatttgc tggacggtct gcgcggtgat
     4741 ttcctcgccg cactgaaagc aggaggggcg tccaacccgg gcgaagagaa gacgtaggta
     4801 atcgtagatc tccgtcacgg tgccgaccgt ggatcggggg ttgtggcttg tgctcttttg
     4861 ctcgatggaa atggcaggtg agagcccttc gatcgaatca acgtcgggct tgcccatctg
     4921 ttcaaggaac tgacgggcat aggccgagag tgactcgaca taccgccgtt gcccttcggc
     4981 atagatcgtg tcgaaggcca gcgacgattt gcctgacccg ctcagccccg tgatgacgac
     5041 gagtcggtcg cgcgggatcg tcacgtcaag gttcttgaga ttatgctccc gtgccccttt
     5101 gataacgatc gagttatgca tcaggggcgg gattatacca tgcagttcta cctgtcttga
     5161 caaaccataa aggccggtgc taccacgctc tcatggtaca tgtggatata ccgcccgttc
     5221 agaagggaac agactccccg gcggctccag tcgccgtgtc gacctggtcg ggccaagccc
     5281 gagaacaagc cgttcagcga atgttcacct cgattgccgg ggtctatgac ctgaacaaca
     5341 cactcctgag ttttggactc cactaccggt ggaagaccat tacggcctcc tatgtgcccg
     5401 tcaccacagg aggccgggca ctcgacgtcg gctccggcac agccgatctc gcgctcttga
     5461 tcgagccgcg catgggaacc ggcggtcgtg tcattgcctc cgacctgaac cacgccatgc
     5521 ttgtcgaggg actgaagaaa gtcaccgcgc ggggcttggg agagcggatc acctgtcttg
     5581 aagccaacgc cgagcacctg gggtttcccg atcggacgtt tcatgccgtg accacgggat
     5641 tttgcatgag gaacgtcggt gatctccggc aagcgctctc ggacattcac cgtatagtgc
     5701 agccgggtgg gcgattcgta tgcctggagt tttcacggcc ggtctttggt tggctccggg
     5761 cactgtacga ttggtactca tttcggcttc ttccctggat tggaacaaaa gtggcgcatg
     5821 atacaactgg cgtctatgaa tatcttccgg cctccattcg gaacttcccg gaccaagaac
     5881 gattgaagca gatgctcctt gacgcaggct ttcgtcaggt cacctatcgc aacctgtccg
     5941 gcgggatcgt cgcgatccat gtggcgacga aatagcaaac agcatggagc ttatggcata
     6001 ttgctgaaga cagatgactc tactcttttt tcggttcata gccctacgcc gttagcctcg
     6061 agttccgact atggaatcga ttctctacat ctttctcccc tgcaagaagg tctatcccat
     6121 cggtgtgacc tatctcgcgg attttattca tcggcgcaag ccagaggtgc ggcagcgcat
     6181 tgttgatctg tctctctttc ccgatgccca gcggatcaag gcggttcgcg atgccgcaac
     6241 cgagttcaag ccggacctcg tctgtttctc ctggcgcgac attcagattt tctccccgca
     6301 tgagggagac tcgtcgctcg aacatgcgtt taatttctac tttgcgagca atcctcttaa
     6361 gcgtgtcgtg gcctcgtttg cgggactgca acagctctat cgctactaca gtcatatcag
     6421 ggcggcgctc tcctacccct ggttgatcgc caaggagttc ccgaaggcgc agatcatgat
     6481 cggtggcgga gcctttaccg catttgccga tcaactcatc ctcaaacttc cggaaggtac
     6541 cattgggatt cttggggagg gtgaagacgc catcctcaag gtgattgagg gccaatcgct
     6601 ggagaaagag cgttacatca tccgggaggg aaagaccatt cgtaagggcc agcagggctc
     6661 gcccgctctg ctcgatgccc tcaccgtcga cttgccctac ctgacctcga ttttcccgca
     6721 gtatcaggaa tatctgggtg aatccatcgg ggtccagtcg aagcggggtt gcccctacga
     6781 ctgcgcgttc tgcctgtacc cctacatcga agggaagcga gtccgctatc ggccgcctgc
     6841 catggtcgtg aaggatatct cgcagcacta ccatcaatgg ggcgccagga ggttctggtt
     6901 tacggatgcc cagtttatta ccgggaaaga agcctatccc caatgcatcg agatccttga
     6961 acggatcctt gccgagaaac tggagatcga gtggtctggg tatattcgga cgtcgctcat
     7021 caccccggag ttggcaaaac tcatggttcg gtcaggcgtc ggcgacctcg aggtcgccat
     7081 tacgtcaggc tcccaagtgg tgttgaacga cctccatatg ggattcaagc ttgagcgact
     7141 gtacgatggt tgtcgctatc tggcagaagc gggattcaag gggaaggtca tcctcaatta
     7201 ctcgttgaat agtccaaaag aaaccgagga gtcattgctg cagagcgtgg tggtagacgt
     7261 aaccgtgaaa aggatggggg tggctcgcgc gctgatgagc gtgatgtaca tatccttcac
     7321 tagc
//
LOCUS       DATIPF010000309         4744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1653431, whole genome shotgun sequence.
ACCESSION   DATIPF010000309 DATIPF010000000
VERSION     DATIPF010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653431"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VLL06_07695"
     CDS             <1..232
                     /locus_tag="VLL06_07695"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS30885.1"
                     /translation="EGGPLEEIVEHLTVATQLDPTHIASHLALGRALIKLGKKDEARP
                     ILTAGIDAALSGRSNGGGDLVPELQQLLRTLG"
     gene            261..461
                     /locus_tag="VLL06_07700"
     CDS             261..461
                     /locus_tag="VLL06_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30886.1"
                     /translation="MNLDEARTRLETAVTQYGQHAAPAIDLVMSEVRSDLGKSAFNEL
                     VDEFDLELLYGIAPMETDHSSS"
     gene            482..1579
                     /locus_tag="VLL06_07705"
     CDS             482..1579
                     /locus_tag="VLL06_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30887.1"
                     /translation="MPLIWAAISAHGFGHAAQVVPVLNALGRFIPDLRAILRTTVPPS
                     FFSDRLIIPWELSASQQDIGCIQDGPLSINVEATWRAHERFHTTWPDRIQHEVTAMKA
                     AHPNVVLANTPYLAIEAGKAAGIPTVALMNFTWDLVLSEYIAPPPMDGSSLLGSIRRS
                     YANADRALRMTPAPKIDTFQHIDTIGPITEPASSVRDQLGSALSLAPGERTVLVGFGG
                     IPLTSLPLADLEQLTGYRFLFDGPILPGSTTFISIHSLPFSFKSLMASVDVIMTKPGY
                     GTIVEAVALHQPVVYVRRYNFADEPPLVDYLHRYGRGVELSIDDFTQGRWEPVLKQIL
                     ALPQPAFMPPASSGAKDAATILAPYLTKSGL"
     gene            1582..2412
                     /locus_tag="VLL06_07710"
     CDS             1582..2412
                     /locus_tag="VLL06_07710"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSS30888.1"
                     /translation="MRLFGLVTRYVSNGCKRIGGAFFLIAQRLDALLPALFSPTHLSR
                     LTHVHYEDSYRDASGRYSTEAYGTGLQEWEEEVLAHHKIVSGITMVLGAGVGRESVEF
                     ARRGLRVIGLDISRDALRLAALIAQKRSTHITLVQASFYDIPTLTARIDYIFLSGIMY
                     SSIPGRQARQAWLRRLQTHLRSGGLAVLNYSIYLDRTVPLTNRPAYTLNSWLAGLPGA
                     NPAYQPGDLVSHDHFLHSFLNEEELRSELTEAGATIVQLNWDKQFAVLTWLHEPVCNG
                     "
     gene            2484..2795
                     /locus_tag="VLL06_07715"
     CDS             2484..2795
                     /locus_tag="VLL06_07715"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HSS30889.1"
                     /translation="MNATAVYARHPDYVQRDVAGECILVPIRRSLAEANSIYVLNETG
                     AALWNRLDGRQRLQDITADFCVEYDVATDQLHRDLETLLADLLSIHAIEEVAVADGQT
                     G"
     gene            2779..3690
                     /locus_tag="VLL06_07720"
     CDS             2779..3690
                     /locus_tag="VLL06_07720"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSS30890.1"
                     /translation="MVRRDKLHHLPERFPVACQWEITCRCNLHCVMCYTDCFNRPNLI
                     REELTTPHILRIMDEMADAGTLELCLTGGEPMIRPDFFHIYDHAIRRGFLVTVFTNGT
                     LITEAAADRFAALRPHRIEISLHGLTKETFERITQGTGSYHRCLDGIKLLIDRQVPLT
                     LKSTAMTLNQHEILAIKQYVASLGSVSYKLGEEMRPELDGGAGPFHYALSEGDVRILN
                     QQDDDLWKESCRQEGTDPAPCRSGMQRFHIDAYGKLQLCSGNRLQSYDLRTGSFREGF
                     FEAMPTFACEWKAPLASEPIHPTVYHA"
     gene            3710..>4744
                     /locus_tag="VLL06_07725"
     CDS             3710..>4744
                     /locus_tag="VLL06_07725"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSS30891.1"
                     /translation="MPYGEFSRMAYERSLQQDRIIKAQLELTYRCNLHCRHCYTDPYN
                     SKEFFPRELSITDIHRLLREMRELGVVWLNLTGGDIFMHPDFFEIYEAAYQKGFLLQL
                     YTNGTLFTRAIIERLKSMPPFTIDISCHSVNEERFDWFTQVSGSYRAFMRGVTLLQES
                     GLPFMLKTKLMDWNKDELDDIRAFTESFGQRFGYTTSLSPRLNGDCTSLAYRIDPQDL
                     RKLEHIDAPREAEERCAAQEEIVGPDTERLYRCGCGTNSIHINAWGELGTCTLQYEHR
                     VSLRARPLKDAVAEVFAAVRSRHYHSDSPCRSCAIHRFCEKKPTDARWEQGDAEAPIP
                     YDCDTALVRAE"
BASE COUNT         1025 a         1472 c         1245 g         1002 t
ORIGIN      
        1 ggaaggcggg ccactcgaag agatcgtcga gcacttgacc gtggccacgc agctagatcc
       61 aacccacatc gcatcgcatc tggccctggg ccgcgcgctt atcaaactgg gcaaaaagga
      121 tgaggccagg ccgatcctca ctgccggcat cgacgccgcg ttatcgggcc gatccaacgg
      181 tgggggtgat ctcgtgccgg agctgcagca gttgctgcgc acgcttggat aaacaggctt
      241 tcacgactag ggagtccact atgaatcttg atgaagccag aacacgcctt gaaacagccg
      301 taactcagta tgggcagcat gccgccccgg cgatcgattt agtcatgagc gaagtccggt
      361 ctgacttagg caagagcgcg ttcaacgaac tggtcgacga gttcgatctg gaactcttgt
      421 acggtatcgc tccgatggaa acggaccact cgagcagcta acccgtaaga gtgacctcgc
      481 cttgccactg atctgggcag cgatttccgc ccatgggttt ggtcacgccg cgcaggtcgt
      541 gccggtcctc aatgctctcg gcaggtttat tcccgacctg cgcgccatct tgcgaaccac
      601 cgtccccccg tcattcttca gcgatcgcct gataatcccc tgggaactca gtgcttcaca
      661 gcaagacatc ggctgtatcc aggacgggcc cctgtcgatc aatgtggagg cgacctggcg
      721 tgcgcacgag cgatttcata cgacttggcc cgaccggatt cagcatgaag tgacggcgat
      781 gaaggctgcc catccgaatg tggtccttgc caacacaccc tatcttgcca tcgaggccgg
      841 aaaggccgca ggaattccca ccgtcgcgtt gatgaacttc acctgggacc tggttctctc
      901 ggaatacatc gctccgccac ccatggacgg atcgtcgctc cttggttcaa ttcggcggag
      961 ctacgccaat gccgatcgtg ccttgcgcat gacgcctgcg ccaaagattg atacctttca
     1021 gcacattgat acgattggac ccatcaccga gccggcctct tcggttcgcg accagcttgg
     1081 ctcagcgctt agtctggccc ccggggaacg gacggtgctc gtgggatttg gcggtattcc
     1141 actgacctca ctcccgcttg ctgatctgga gcaactcacg ggttatcggt ttctcttcga
     1201 cggacccatt ctaccgggca gtaccacatt catatcgatt cactcactac ccttttcatt
     1261 caaaagcttg atggcctctg tcgatgtcat catgacgaaa cccggctacg gcactatcgt
     1321 tgaggcggtc gcactgcatc agccggttgt ctacgtccgc cgatataact ttgccgatga
     1381 accgccgctg gtcgactatc tgcatcgtta cggacgcggc gtcgaattgt cgatcgacga
     1441 cttcacgcaa ggccgctggg agccggtgtt gaagcagata ctggcactac cccaaccggc
     1501 cttcatgccg ccggcgtcgt ccggcgcgaa ggatgccgcc actatccttg ccccctatct
     1561 caccaaatca ggactatgac catgcgtctg tttggacttg tgactcgata tgtctccaac
     1621 ggttgcaaac gaatcggagg ggccttcttt ctcatcgctc aacgactgga cgctctcttg
     1681 ccggcgcttt tctcccccac tcacctgtcc agactcacgc acgtgcatta cgaagacagc
     1741 tatcgtgatg cttccggacg ctattccact gaggcctatg gaacggggct tcaagagtgg
     1801 gaagaagagg tgctcgcgca ccacaagatc gtgtccggca taaccatggt gcttggagcc
     1861 ggagttggcc gtgaatccgt cgaatttgcc aggcggggcc tgcgtgtgat cggacttgat
     1921 atcagtcgag acgctctccg actggccgcc ctcattgcac agaaacgctc cacacacatc
     1981 acccttgttc aagcgagctt ctatgacatc cccaccctca cggcccgcat tgattacatc
     2041 tttctctccg gcatcatgta cagctcaatc cccggacggc aggcgcgaca agcgtggctc
     2101 cgaagactgc agacccatct gagatcgggt ggactcgccg tcctgaacta ttccatatac
     2161 cttgaccgga cagtacctct gacgaatcgg cctgcctaca cactcaattc ctggctggcc
     2221 ggcctgcccg gggcaaatcc cgcataccaa ccgggcgatc tcgtgtctca cgatcacttt
     2281 ctccactctt tcttgaacga ggaagagctt cgctccgagc tgacagaagc aggcgcaacg
     2341 atcgtccaac tcaattggga caaacagttc gccgtgctca cctggcttca cgaacccgtc
     2401 tgtaacggat aatcgagagg cggccgaatt cggcgagaaa tacctctgga agcggcctag
     2461 gggatttgct atcctcctgc tccatgaacg ccaccgccgt ctacgccaga catcctgatt
     2521 atgtgcagcg agacgtcgcg ggagaatgta ttctcgttcc catccgccgg tccctcgcag
     2581 aagcgaacag tatctacgtc ttgaacgaaa cgggagcggc cctctggaat cgccttgacg
     2641 gcagacaacg gctgcaagac atcacggcgg acttttgcgt cgagtacgac gtcgcaacag
     2701 atcaattaca ccgggatctt gagaccctcc tggcagacct actttcgatt catgccattg
     2761 aagaggttgc cgttgccgat ggtcagacgg gataaactcc atcacctccc cgaacggttt
     2821 cccgtcgcct gccagtggga aatcacctgc cgctgcaacc tgcattgcgt catgtgttac
     2881 acggattgtt tcaatcgtcc caacctcatc cgtgaagaac tcaccacccc tcacatcctt
     2941 cgcatcatgg acgagatggc cgacgccggc acgctcgaac tgtgcttgac cggcggcgag
     3001 ccgatgatcc gccctgactt ctttcacatt tacgatcacg ccatccgccg cgggtttctc
     3061 gtgacggtat ttacgaatgg cacactgatc acggaagctg ccgcggaccg ttttgcggca
     3121 ctccgtcccc atcggatcga gatcagtctt cacggcctga caaaggaaac attcgagcgt
     3181 attacccaag gcaccgggtc ctatcatcgc tgtctggacg gcatcaagct gctgatcgat
     3241 cgacaagtcc cgctgacact caagagcaca gcgatgacgc tcaaccagca tgagatcctg
     3301 gcaatcaaac agtacgtcgc ctcgcttgga tcggtcagct acaaactcgg ggaagagatg
     3361 cggccagaac tcgacggcgg agccggcccg tttcactacg cactctccga gggagacgtg
     3421 cgaatcctca atcaacaaga tgacgatctg tggaaggagt cctgccggca ggaaggtaca
     3481 gatcctgccc cctgccgaag cgggatgcaa cgatttcata tcgacgccta tgggaagctg
     3541 caactctgtt caggcaatcg tctgcaaagc tacgatcttc ggaccgggtc gttcagggaa
     3601 ggtttcttcg aggccatgcc cacgtttgcg tgcgagtgga aagcacctct cgcatcggaa
     3661 ccgatccacc cgacggtgta ccatgcctga attgagaccc ctacccacca tgccgtatgg
     3721 cgaattcagc cgcatggcct atgagcgttc actgcaacaa gaccgcatca tcaaagccca
     3781 actcgagctc acctatcgct gcaatttgca ctgccgccat tgctacacag atccctacaa
     3841 cagcaaggag ttcttcccac gggaattaag cataacggac attcatcgcc tcctcaggga
     3901 gatgcgagag ctgggtgtgg tctggctcaa tctcaccggt ggcgacatct tcatgcatcc
     3961 tgatttcttc gagatctatg aggcggccta tcagaagggc ttcctgctcc agctctatac
     4021 aaacggcacc ctctttaccc gcgcgatcat cgagagactc aagagcatgc ccccttttac
     4081 catcgatatc tcttgccact cggtgaacga ggagcggttc gactggttca cgcaagtgtc
     4141 gggatcgtat cgcgcattca tgcgcggggt gaccttgctg caggagagtg ggttaccttt
     4201 catgttgaaa accaaattga tggattggaa caaagacgaa ctcgacgaca tccgggcctt
     4261 caccgaatcg tttggccagc ggttcggcta taccacgtcg ctctccccac ggctgaacgg
     4321 cgattgcacg tcgcttgcgt atcgaatcga cccgcaagac ttacggaagc tggaacatat
     4381 cgatgccccc agagaagccg aagagcgttg cgccgcacaa gaggagattg tcgggcctga
     4441 caccgagcgc ctctatcggt gcgggtgcgg gaccaactcc attcacatta atgcctgggg
     4501 cgaactcggc acctgcacgc ttcaatatga acaccgagta tccctgcggg cacgtccact
     4561 caaagatgca gtcgcggagg tattcgcagc cgttcgttca cggcactatc acagcgacag
     4621 tccttgccgt tcctgtgcta ttcaccgctt ttgcgagaag aagccgaccg atgcccgatg
     4681 ggaacagggc gacgctgaag cccccatccc ctacgattgt gacacggcgc tggtccgtgc
     4741 ggaa
//
LOCUS       DATIPF010000310         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_944465, whole genome shotgun sequence.
ACCESSION   DATIPF010000310 DATIPF010000000
VERSION     DATIPF010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944465"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VLL06_07730"
     CDS             complement(<1..505)
                     /locus_tag="VLL06_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HSS30892.1"
                     /translation="MGIEIAITVAREETRVAVLDGGVVTDLFGDRAKHKDFVGNIYKG
                     KVAKVLPGMQAAFVDIGLAKAAFMHVSDLSLDAEPGDTLVDADEDDKDADKDGDMLGP
                     RRQSSKPIEQLLSEGQELMVQISKGPIGTKGSRVTTYVSLPGRYLVFMPNVEHIGVSR
                     RIARDEER"
     gene            complement(676..1791)
                     /gene="rodA"
                     /locus_tag="VLL06_07735"
     CDS             complement(676..1791)
                     /gene="rodA"
                     /locus_tag="VLL06_07735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HSS30893.1"
                     /translation="MIDRVITSRGVDNFDFRFIGLVFAILGLGVLSIYSVTHDQGVAF
                     PFYAKQLVWILLGTVAFLVMWLSDYHRIARLAYPAYAIILILLVIVLFEGKSSRGAQR
                     WIPMGPFAFQPSEFAKLILILTLAYYYSERPRVGWLQRVVLPGLLVLPGLLLILKQPD
                     LGSGLSFVAVYAAMLLMVGMRSKAVGVILLFSLMLFPFAWEMMWGSLHDYQRQRIMTF
                     VDPVSDPGGKGYHALQSQIAIGAGELTGKGLYGGTQSQLKFLPEGHTDFVFSVFAEEW
                     GFLGVLALLILFVALIWLSLEIVARAKDQLGALLAVGILCMLCFCVVINIGMTVGMFP
                     IVGIPLPLMSYGGSATVMTMASLGLLLNVKRRRLSFF"
     gene            complement(1788..>2988)
                     /gene="mrdA"
                     /locus_tag="VLL06_07740"
     CDS             complement(1788..>2988)
                     /gene="mrdA"
                     /locus_tag="VLL06_07740"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HSS30894.1"
                     /translation="EKRTVVVEQPHAGDNLYLTIDARLQKAAEHLLGEETGAIVALDP
                     RTGDVLAMASRPGFDPNVLSRELTPKQWADIVQDEGRPLNNRASQGQYPPGSVFKVMM
                     AAAALETKTVTPETSIFCNGGYQFGRRLYHDWKAGGHGAVDLRRALVQSCDVYFYTIG
                     QRMGIETMASFAHQFGLGEETGIELPSERVGIVPSEAWKRKAKNEPWLPGETISASIG
                     QGYVNVTPLQMASLIGTVANDGVTFRPRLVQAVLDRATGELQPRPAVPKRTVTLKPGI
                     LSRIREALAGVVKEGTGTRAQSALVTIGGKTGTAQVAALRTGPEKDIPKKFRDHAWFV
                     AFAPVEAPTIAVAVLGEHLGHGGSASAPLAKELIETYIKLDLPASSGMTDGAVARERD
                     AGGQGTR"
BASE COUNT          690 a          999 c          725 g          574 t
ORIGIN      
        1 cccgctcctc gtcacgggct atgcggcggg agacaccgat atgttccaca ttgggcatga
       61 acacaaggta tcgcccgggg agcgacacat acgtcgtgac ccgcgagcct ttcgtgccga
      121 taggcccttt ggaaatctgc accatcagct cttgcccctc gctcagcaac tgctcaattg
      181 gcttggagct ttggcgccgg gggcccagca tatccccatc cttatcggca tccttgtcgt
      241 cctcatccgc atcgacgagc gtgtcgcccg gttcggcgtc caacgataga tccgacacat
      301 gcatgaacgc cgccttcgca aggccgatat cgacaaatgc cgcctgcatc ccaggcagca
      361 ctttcgccac tttcccctta taaatgttcc cgacaaagtc tttgtgcttc gcacgatccc
      421 cgaagagatc cgtcacgact ccgccgtcca acaccgcgac gcgagtttcc tcgcgtgcga
      481 ctgtaatcgc aatttcgatt cccataccca ctccttccat tcagcaaaac cgtcgcgccg
      541 atgtgcagga cgaacgcggg gaatccccag gttccggcct ccactcgact gctcgtcggc
      601 tcgacggttt gcaccataat ataaaaccgc gtgcatcggt cccgatttat gcgagaccgt
      661 gcgatgaccc tctcactaaa agaaactcaa tcgacgacgt ttgacattca agagcaagcc
      721 gagcgacgcc atggtcatca ccgtcgcact cccgccataa ctcatgagcg gaaggggaat
      781 tcccacgatg gggaacatgc ctaccgtcat gccgatattg atcaccacac aaaaacacaa
      841 catgcagagg ataccgaccg ccagcagcgc cccgagctga tccttcgctc gcgccacaat
      901 ttccaacgac agccagatca aggcgacaaa taatatcaag agcgccagca ctccgagaaa
      961 tccccattcc tctgcaaaca ccgaaaagac aaagtctgta tgcccttctg gcaaaaactt
     1021 caattgactc tgcgtccctc catagagccc cttccccgtc aactctcccg ccccgatggc
     1081 aatctgcgat tgaagggcat ggtatccctt gcccccggga tcggagaccg gatcgacaaa
     1141 cgtcataatg cgctgccgct gataatcatg caatgacccc cacatcatct cccaggcgaa
     1201 gggaaacagc atcaacgaaa acaggagaat gacacccacc gccttcgaac gcatgcccac
     1261 catcagcaac atcgcggcat agactgccac aaagctcaac ccacttccga gatccggctg
     1321 cttcagtatg aggagcaggc ccggcagtac caacagcccc ggaagcacca ctcgctggag
     1381 ccaaccgacc cgcggcctct ctgaatagta gtacgccaag gtcagtatca ggatcagctt
     1441 ggcgaattcc gaaggctgaa aggcaaatgg ccccatcgga atccatcgtt gagcccctcg
     1501 gctgcttttg ccttcaaaca atacgattac gagcaagatt aagataatcg cgtaggcagg
     1561 atacgctaag cgtgcgattc gatgatagtc cgaaagccac atgacgagaa acgcgaccgt
     1621 gccgagcaag atccacacaa gttgtttagc ataaaacggg aacgcaaccc cctggtcatg
     1681 cgtcacgcta taaatggaca gaacacccag ccccaggatg gcaaagacca gtccgataaa
     1741 acggaaatcg aagttatcga cgccacggct cgtgatcacc cggtcgatca tctcgtcccc
     1801 tgccctccgg catcccgttc ccgcgccacc gcaccatcgg tcataccgga agaagcggga
     1861 agatccagtt ttatataggt ctcgatcagc tctttggcaa ggggcgcgga ggcagacccc
     1921 ccatggccca agtgttcacc cagcaccgca acggcaatgg tgggcgcctc gaccggagcg
     1981 aatgcgacaa accaggcatg atcgcggaac ttcttcggaa tatccttctc tggaccggtg
     2041 cgcaaggccg ccacttgagc cgtaccggtc ttcccgccga tcgtcaccag cgccgactgc
     2101 gcccgcgtgc ccgtcccctc tttcaccacc ccagccagcg cctctctgat ccgcgagaga
     2161 atcccgggct tcaatgtcac cgtacgcttg ggaaccgcag gccttggctg gagttctccg
     2221 gtcgctcgat ccaacacggc ctgaaccaat cgaggacgga acgtgacgcc atcgtttgca
     2281 acagtcccga tcaaactcgc catctgcagc ggggtcacat tcacatagcc ctgtccgatt
     2341 gatgccgaga tcgtttcgcc tggcagccac ggctcattct tggccttccg tttccacgct
     2401 tcagaaggca cgatcccaac ccgttccgac ggcagctcaa tccccgtctc ctctcctaac
     2461 ccaaattgat gggcgaagga cgccatcgtc tcgatcccca tccgctgacc gatggtgtag
     2521 aaatagacgt cgcacgattg cacgagcgcg cgcctgagat ccaccgcacc atgtcccccg
     2581 gctttccagt catgatacag gcggcgaccg aattgatacc cgccattgca gaagatggat
     2641 gtctccggcg tcaccgtctt ggtctctaat gccgcagcgg ccatcatcac cttgaatacc
     2701 gaaccgggag gatactgccc ttgcgaggct cgattgttca agggacgccc ctcgtcttgc
     2761 acgatatcag cccattgttt cggtgtgagc tcccgcgaaa gcacgttcgg atcgaaaccg
     2821 ggacggctgg ccatggccag cacatcgcct gtcctcggat caagcgcgac aattgcgcct
     2881 gtttcctctc ccaacaggtg ttcggccgct ttttgcagcc gcgcatcgat cgtcagatac
     2941 aagttgtccc ccgcatgggg ctgttccacg accacggttc gtttctcg
//
LOCUS       DATIPF010000311         3301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1562165, whole genome shotgun sequence.
ACCESSION   DATIPF010000311 DATIPF010000000
VERSION     DATIPF010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562165"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VLL06_07745"
     CDS             <1..298
                     /locus_tag="VLL06_07745"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HSS30895.1"
                     /translation="AQCGANRGTFGSRIMAEWGNLGKVFIGVGVCVALVGLLILIADR
                     VPGIGNLLGWFGKLPGDISIRRDNFSFSFPLVTSLLLSVILSVILYFITWIFRR"
     gene            295..1509
                     /locus_tag="VLL06_07750"
     CDS             295..1509
                     /locus_tag="VLL06_07750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HSS30896.1"
                     /translation="MKQLKANMYRTVVAGVCLFSLAFGAEAAEPIRVLLSADVLRLEI
                     RTDGPLWVTDAKGRGQTLRTPVQVTAAEAGFLMNGVRLQTEQVTLQGGEQGLTLTFPR
                     PVRKSNGAAVSSGDSGTEVSVSGLVQFVRRGKGFSVMNRVDLEEYVKGVVPSEVSSTW
                     HPEMLKAQAVAARTYALYQQMLSATREYDVAATVQDQVYRGKQGIDVGILRAVEETRG
                     LVVTYLGAPIYAAFSSTAAGLTEDAMNVWSKEYPYLKGVECPFDLASPYYKWKSSFKI
                     DTLEQNLRQQGFPVGTIATITPLVFSRGGRVAKLRVLHSGGELVLRGEELRKAVGYTI
                     IPSTQFAIESIGQDVVLSGFGAGHAVGMCQWGAKELAELGYPFSTILQYYYPGTELQN
                     LTLTKPPTPPSS"
     gene            1509..2528
                     /gene="queA"
                     /locus_tag="VLL06_07755"
     CDS             1509..2528
                     /gene="queA"
                     /locus_tag="VLL06_07755"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HSS30897.1"
                     /translation="MQLSEFDFPFDPSLIALQPCFPRDHARLLVLQALDGSWTHRRID
                     ELPDLLQPGDLLVVNNTKVLAARVVGRKCPSGTEVEILFVKDLGDAIWEVLIKGTFRP
                     GQMIEMGADVSALVVERGARRTTVRIESPIPWSEWLRQHGRMPLPPYMKRAPTEQDRQ
                     WYQTLFAEHEGAIAAPTAGLHFTQELLARLQRRGVGLATVTLHVGVGTFKPVTVDHIE
                     DHQMGAEWLEVGAEAVRAIEQVRAKGGRIVAVGTTVVRALETAARAEGRIRSYRGETD
                     MFMTPGSSFKVVDALLTNFHMPRTTLLMLVAALAGTEFLRRAYAEAVRERYRFYSYGD
                     AMLIL"
     gene            complement(2645..>3301)
                     /locus_tag="VLL06_07760"
     CDS             complement(2645..>3301)
                     /locus_tag="VLL06_07760"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HSS30898.1"
                     /translation="LLKSVMGVIALTFVITMGWWGFGEQTGTVVASVGDLTVSHDEFR
                     RAYENTYRFYKDKVPGEFKDETIKQLVVDQLIDNRTWLIAAKNMGLTVSNDDLREVIM
                     QIPDFQKNGTFDPEVYKRLLAANHLTPAIFEAMEAKEVLSNKARMIIRDSVALTPAEL
                     AEAQALTLRQTESDPAKAAAAKDRAMQDVLFQKQQRALMAYTQSLKTSIPIKIHRELL
                     "
BASE COUNT          659 a          842 c         1016 g          784 t
ORIGIN      
        1 cgctcaatgc ggtgcaaatc gcggaacatt tggctcaagg ataatggctg aatggggaaa
       61 tcttggcaag gtcttcatcg gagtcggggt atgcgtggcg ctggtcgggt tgttgatcct
      121 gatcgcagac cgcgttcctg gaattgggaa tcttctggga tggttcggca agctccccgg
      181 cgacatttct attcgacgag ataacttcag cttctcgttt cccctcgtca ccagcctgct
      241 gctcagtgtc atcctgagcg tgatactcta tttcatcacg tggatatttc gccggtgaag
      301 caattgaagg cgaacatgta tcgaaccgtt gtggcgggag tgtgtctatt cagcctggcc
      361 tttggggcag aggcggcgga gccgatcaga gttctactgt cggccgacgt gctacggctc
      421 gagatccgta ccgacggtcc gttatgggtg acggatgcaa aaggtcgtgg tcagactctt
      481 cgcacgccgg tccaggtgac agctgctgaa gcggggtttc tgatgaatgg tgtccgcctg
      541 cagactgaac aggtgacgct gcaaggcggg gaacaaggtc tcacgctcac gtttccacga
      601 ccagttcgga agtccaatgg ggcggcggtg tcatcgggcg actccggcac ggaggtctca
      661 gtgagcggcc tggtccaatt tgtgcgaaga ggcaagggat tttctgtcat gaatcgggtg
      721 gaccttgaag agtacgtcaa aggggtcgtt ccctcggagg tcagttcgac ctggcacccg
      781 gaaatgctga aagcgcaggc ggtcgctgca cggacgtatg cgctgtacca gcagatgttg
      841 agcgccacgc gggagtacga cgtggcggca acggtgcaag accaagtcta tcgtgggaag
      901 caggggattg atgtggggat cctgcgtgca gtcgaagaga cccgcgggtt ggtcgtcacc
      961 tatctgggtg caccgatcta cgcagccttt tcatcgacag ccgctgggct gacggaagat
     1021 gccatgaatg tgtggtcgaa agagtatccg tatctcaaag gtgtggagtg tcctttcgac
     1081 cttgcgtcgc cctactataa atggaaatct tccttcaaaa tcgacacgct cgaacagaat
     1141 ttgcggcaac agggatttcc cgtgggtacg atcgcgacga tcacgcccct ggtattcagc
     1201 cgcggggggc gagtcgcgaa actgcgtgtg cttcactctg gcggagaatt ggttcttcgt
     1261 ggagaagagt tacggaaagc ggtcggctat accatcatcc ccagcacgca gtttgccatc
     1321 gaatcgatcg gtcaagatgt ggtgttatcg gggtttggtg cgggtcatgc tgtcggcatg
     1381 tgccagtggg gggccaaaga actggctgag ttgggctacc ccttctcgac gattcttcag
     1441 tattactatc ctggtaccga actccaaaat ttgacgctga caaaacctcc gactccgccg
     1501 tcttcctgat gcagctctcc gaattcgatt ttccgttcga tccctcgcta attgcccttc
     1561 aaccatgctt cccgcgcgat cacgccaggc tgcttgtttt acaggccctt gatggttcgt
     1621 ggacccatcg tcgcatcgac gagttgcctg atcttcttca accaggcgat ctcctcgtgg
     1681 tcaacaatac gaaggtgttg gcggcgcgcg tggttggacg gaagtgtcct tctggtacgg
     1741 aggtcgagat cctttttgta aaagatcttg gcgatgcgat ctgggaggtg ctgatcaagg
     1801 gaacgtttcg gcctggacag atgattgaga tgggggcaga tgtatctgct ctggtggtcg
     1861 agcgtggcgc acgacgaacg acggttcgga ttgagagtcc aattccgtgg tccgaatggt
     1921 tgcgacaaca tggtcgaatg ccgcttcctc cctatatgaa acgggcaccg accgagcaag
     1981 atcgacaatg gtaccaaacc ctgtttgcag aacatgaggg tgccattgcg gcgccgaccg
     2041 cagggctcca ttttactcag gaactgctgg cacggctgca acggcgaggg gtcggtctcg
     2101 cgaccgtgac gctccatgtc ggtgtcggaa ccttcaagcc tgtgacggtc gatcacatcg
     2161 aagaccatca gatgggcgcg gagtggcttg aggttggagc agaggcggtt cgtgccattg
     2221 agcaggtgcg ggcgaaggga ggacgcattg ttgctgtcgg gaccacggtt gtgcgcgccc
     2281 tcgaaactgc ggctcgtgcg gagggccgga tccgatccta tcggggagag acagacatgt
     2341 ttatgacacc agggtcttcg ttcaaggtgg tcgacgccct ccttacgaat tttcacatgc
     2401 cacgtaccac gttgctgatg ctggtggccg cattggccgg gaccgaattt cttcgacgag
     2461 cctatgcgga agcggttcgc gaacgttacc gcttttacag ctatggtgat gcgatgttaa
     2521 ttctctagca ggatgctcct gttgtcccag atgtacagac aaatgaaatt ctggcgtgcc
     2581 ccaaagttcc tcaccgaggc ctgtcaaatc tcacgacccg acgaggatat tcgccgaatg
     2641 ggggttacaa caactcgcgg tggatcttga tggggatgct agttttgagc gattgagtgt
     2701 atgccatcaa cgcgcgttgc tgcttttgaa agaggacgtc ctgcatggcg cggtcctttg
     2761 ctgctgctgc cttcgcggga tctgattccg tttgccggag tgtgagggct tgtgcctcag
     2821 caagctcggc aggggtaagg gccacggagt ctcggatgat cattcgagct ttgttgctca
     2881 gcacttcctt cgcttccatc gcttcgaaga tcgcgggcgt cagatgattc gctgccagga
     2941 gccgtttgta gacctccgga tcgaacgtgc cgttcttttg gaagtcaggg atctgcatga
     3001 tgacttcacg caggtcgtcg ttcgacaccg tgaggcccat gttcttggcc gcgatgagcc
     3061 aggtccgatt gtcgatgagt tgatccacca cgagttgctt gatcgtttca tctttgaatt
     3121 ctccaggaac tttgtcctta tagaaacgat acgtattctc ataggcgcgt ctgaactcat
     3181 catgggacac ggtgagatct ccgactgagg cgacgacggt accggtctgt tcgccgaatc
     3241 cccaccagcc catggtgatg acgaaggtaa gggcgatgac ccccatgacg gacttgagga
     3301 g
//
LOCUS       DATIPF010000312         3495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_944812, whole genome shotgun sequence.
ACCESSION   DATIPF010000312 DATIPF010000000
VERSION     DATIPF010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944812"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..877
                     /locus_tag="VLL06_07765"
     CDS             80..877
                     /locus_tag="VLL06_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSS30899.1"
                     /translation="MHPILDIQHAMIYRGDRRVFSDFSFALHEGEHAAIVGPNGAGKS
                     TLLKLLAGEVHPVPLDETSIRLFGEEQANVWDVRKRLGMVSHDLQHQYMDQVTGLKVI
                     LSGFYASIGTYGHQDFSYAQIARADEMSEEMGVGSLKDRRFVEMSTGEQRRCLLGRAL
                     VHEPTALVLDEPTSGLDLTATFHYLDLVRMHMRKGKTLLLVTHQIHEIPPEVERVVLL
                     KQGRILQDGDKRAVLTDGNLSMLFDCPVALAQANGWYQALPGHSIPH"
     gene            complement(888..1334)
                     /locus_tag="VLL06_07770"
     CDS             complement(888..1334)
                     /locus_tag="VLL06_07770"
                     /inference="COORDINATES: protein motif:HMM:NF016798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUN14 domain-containing protein"
                     /protein_id="HSS30900.1"
                     /translation="MDDEYEQPHASSSQGIGAAILADAPWNAKSVIAAGIATAGSLAG
                     WIADYSSPVLVGIGASYLAGFFLGWASRRFLRMAALITGGLLASIAVLEETGWIDFDW
                     TTVETQINHAVAAAQQGAQGLKQILSGYLPSTGAAAAGMFFGFRKK"
     gene            complement(1341..2201)
                     /locus_tag="VLL06_07775"
     CDS             complement(1341..2201)
                     /locus_tag="VLL06_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HSS30901.1"
                     /translation="MRWEGQRESDNVEDRRGMGPARVGGGRLGIGGLVLVLAVSYFTG
                     INPLTLIDMLNGVQDMTESSGPSEPAPIGAPNDQLGKFAAIVLADTETTWRQLLGPQY
                     EYPRLVLFTGGVHSACGTTSSAVGPFYCPNDRKVYLDLSFFNAMARRFGAPGDFAQAY
                     VIAHEVGHHVQNFMGIAEKVTRLRQQASEREGNALSVQMELQADCFAGVWGYHAKRER
                     NLIELGDFEAGLNAAAAIGDDRLQKMGQGYVQPESWTHGSSEQRRTWLRKGLETGDPK
                     ACDTFAGNRL"
     gene            complement(2515..>3495)
                     /locus_tag="VLL06_07780"
     CDS             complement(2515..>3495)
                     /locus_tag="VLL06_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSS30902.1"
                     /translation="LCAIAVGGCVSTGTHTKTLTELEAEKKTSAQLQQQLAGLQKNLD
                     QEAAQRKAAEQEAASLAARSSQLQSRLDGLEKEKGQLNSELGNVRSRITDLEQKLTSG
                     NASSQEEIARLQKQASDLEADAARIAKEREQLRQEQSQLAATLERERLAKEEEIGRLT
                     RTQEALSKSLQDEISKGNITIQQVRDRLTINMVDRVLFDSGQAQVKPAGMKVLKQVGD
                     VLNQISDKQIRIEGHTDNVPISSKLQDRFKTNWELSTARATTVVRYLIDQGGVDRQYL
                     SAVGYADTHPLASNDSEEGRSSNRRIEIVLYPKDLKHIAGQLETATAPTK"
BASE COUNT          689 a          957 c         1006 g          843 t
ORIGIN      
        1 cttgacaggt tgcgcgctca ccctctaact ttgccaagct ttatcgtgcc tgtagtttcg
       61 gactggacga ggccctgcca tgcatccgat ccttgacatc cagcatgcga tgatctaccg
      121 gggagaccgt cgcgtcttct ctgatttttc cttcgccctt cacgagggag aacatgcggc
      181 gattgtcggt cccaacgggg ccggcaagtc gaccctgctc aaacttctag ccggagaagt
      241 ccatccggtt ccattggatg agaccagtat tcgcctgttc ggggaagaac aggcgaatgt
      301 gtgggacgtt cgcaaacgtt tggggatggt gtctcatgat cttcaacatc aatatatgga
      361 ccaagtgact ggactgaaag tgattctgtc cgggttctat gccagcatcg ggacctatgg
      421 ccatcaagac tttagctacg cgcagatcgc tcgggcagac gagatgtcgg aggagatggg
      481 ggtgggttca ttgaaagatc ggcgatttgt ggagatgtcg acaggagagc aacggcgatg
      541 tctcctcggt cgcgcgttag tgcatgagcc gacagccctt gtgctggacg aaccgacgag
      601 cggactcgat ctgacggcga cgtttcacta tctggatctg gtgcgcatgc atatgcgaaa
      661 ggggaaaacg ctgctactgg tcacgcacca gattcacgaa attccaccgg aagtggagcg
      721 agtcgtactc ttgaagcaag gcaggattct tcaggatggg gacaaacgcg ctgtcctcac
      781 agacggtaac ctcagcatgt tgttcgactg cccggtcgca ttggctcaag ctaatgggtg
      841 gtatcaagcg ttacctggac acagcatacc gcattgactt cctcttgcta ttttttccga
      901 aatccgaaaa acatgccggc tgctgctgcg ccggttgatg ggagataccc cgaaaggatc
      961 tgtttgagtc cttgagcgcc ctgctgtgcc gctgcgacgg cgtggttgat ctgcgtttcc
     1021 actgtggtcc aatcgaaatc gatccagccg gtctcttcca atacagcgat agaggccagt
     1081 aatccgcctg tgatgagtgc ggccattctc agaaatcgcc gggatgccca gcctaggaaa
     1141 aatcctgcca ggtaactggc gccaataccg acgagaacgg gtgaagagta atcagcgatc
     1201 catccggcaa gacttcctgc cgttgcgata ccggcagcga tgacggattt tgcattccaa
     1261 ggtgcatcgg ccagaatggc tgcacctata ccttgtgaag atgatgcgtg tggctgctcg
     1321 tactcgtcat ccatgatggg ctagagccgg ttgcctgcaa aggtgtcgca ggctttcggg
     1381 tccccggtct ccaagccttt tcgcagccat gtcctccgtt gttcggagga tccgtgtgtc
     1441 cagctttccg gttggacata gccttgtccc atcttttgga gccggtcgtc tccaatggcg
     1501 gcagcggcgt ttaagcctgc ttcaaaatcg cccagctcga tcaggttccg ctcgcgcttg
     1561 gcgtggtacc cccaaacgcc ggcaaaacaa tcggcttgca gttccatttg cactgacaag
     1621 gcattcccct ctcgttcaga ggcctgctgt cggaggcgcg tgaccttctc ggcaatgccc
     1681 atgaaatttt gcacatgatg gccgacctca tgcgcgatta cgtaggcttg cgcaaagtct
     1741 ccgggcgcac caaaccgtcg cgccatggcg ttgaaaaatg acaggtcgag atagacctta
     1801 cggtcattag ggcaatagaa ggggccgacg gctgatgagg tggtgccaca ggccgagtgg
     1861 acacctccgg tgaaaaggac gagtcggggg tattcgtact gcggaccaag gagctgccgc
     1921 caggtggttt cggtgtcggc gaggacaatt gctgcgaatt ttcccagttg gtcgttgggt
     1981 gctccgatcg gtgcgggctc ggagggccca gatgattccg tcatgtcctg cacgccattc
     2041 agcatatcga tgagggtcaa gggattgata ccggtgaaat agctgacagc cagtaccagc
     2101 accaggccgc cgatcccaag ccttccccct ccgaccctcg ccggtcccat gccccgacgg
     2161 tcttcgacat tgtcgctttc tcgctgtcct tcccagcgca tgaggacctc ctgacaaatc
     2221 gcagcatatc gagcgagcgg attaggtcgg ctctttctcc acggtcatca gtgcgagcgt
     2281 caccacgtac atctcagata cctcagtagg ggaaggggat caatttctcc gcagtggtct
     2341 caaagaccga tcacatgatg catcataacc gatgcaggga agagggggaa ggcttcgatc
     2401 aacgcacagt ggttgtgcat gttcgtgcga tgatcgagag aactgggatt gcgctgagag
     2461 gttcgattga agagggaggt gcccacctcg tgagcacctc caaaggacag atggctactt
     2521 cgttggagct gtggcggttt ccaattgacc ggcaatgtgt ttgaggtcct tgggatacag
     2581 cacgatctca attcgccggt tggacgatcg cccctcttcg gagtcgttcg aggcgagagg
     2641 gtgtgtgtcc gcgtagccaa ctgcggagag gtactggcgg tccacacctc cctgatcgat
     2701 caggtagcgc actacggtcg tggctcgcgc cgtggagagt tcccagtttg tcttaaagcg
     2761 atcctgtagc ttcgaactga tgggtacgtt gtcagtatgg ccttcgatcc gaatttgttt
     2821 gtctgagatc tggttgagca cgtcaccgac ctgtttcagg actttcatgc cggccggttt
     2881 cacctgagcc tggccagaat cgaataggac acgatccacc atgttgatcg tcaggcggtc
     2941 gcgtacctgt tggatcgtaa tgttcccctt cgaaatctca tcctgaaggg acttggatag
     3001 cgcttcttgt gtgcgagtca gtcgtccgat ctcttcttct ttcgccagcc gttctcgctc
     3061 taatgtcgcg gcaagttgtg attgttcctg ccgaagttgc tcacgttcct ttgcgatgcg
     3121 ggcggcgtcg gcctcaaggt cagacgcctg cttctgaagc ctggcgattt cctcttgcga
     3181 tgatgcattt ccgctcgtga gcttctgttc gagatccgtg attcgactgc gcacattgcc
     3241 gagttcgctg tttagttgac ctttctcttt ctccaagccg tccaggcgag actgcagctg
     3301 gctagaacgc gctgcgagcg atgctgcctc ctgttcggcg gctttccgct gagcagcttc
     3361 ctggtccaga tttttctgta gtcctgccaa ctgctgctgt aactgtgcgg atgttttttt
     3421 ctcggcctca agctcagtga gtgtcttcgt gtgagtgccc gtgctcacgc atccccccac
     3481 ggcgatcgca cacaa
//
LOCUS       DATIPF010000313         9327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_327876, whole genome shotgun sequence.
ACCESSION   DATIPF010000313 DATIPF010000000
VERSION     DATIPF010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 9327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9327
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327876"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1527
                     /locus_tag="VLL06_07785"
     CDS             61..1527
                     /locus_tag="VLL06_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30903.1"
                     /translation="MTRILPLSDLATSDLLHRCAVIRDQLSRAGLFPSLSDQAPMSGM
                     VAAATTWRIAPTPFPLTKDQLHFFHQLGPQLLSFYRALNRLYADSIRGLQPAWIAGYL
                     DQGKPEALLTYSRMKRFRDQLPAVIRPDIIPTQDGMVITELDSVPGGIGMTACLSALY
                     TDRENDHLSLVGGPEGMVRGFAAMLRSQLVQHTGCVAILVSEEAKDYRPEMTWLAAQL
                     RTIGLDAYCVEPRQIRFTEEGLRITSDDIDQPVALIYRFYELFDLLNIPKSELVQYAL
                     KKDRVTVTPPYKPALEEKLAFALLHHPMLRPFWEQALSSEVFLHLTAIMPRTWILDPA
                     PIPPSATIPGLVLGGRAVMNWRDLATATQKERHMVIKPSGFSELAWGSRGVSVGHDMP
                     QGEWAGAIDNALAAFPHTPYILQEFHKGRLYELDYFDPVTGEMNRMSGRARLSPYYFV
                     SGEKTELAGILATICPADKKVIHGMKDAVMVPCAVAGP"
     gene            1555..1869
                     /locus_tag="VLL06_07790"
     CDS             1555..1869
                     /locus_tag="VLL06_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HSS30904.1"
                     /translation="MAQCRSDKLPLNIEHSPFVSMAITLYHVAWCPDCKEVRQKLADL
                     HMEYEQIVVPDFRPMRKVVQEVSGQYYVPVLKDGDLVLTETDDILDHLEKTYGQERIA
                     GS"
     gene            1904..3028
                     /locus_tag="VLL06_07795"
     CDS             1904..3028
                     /locus_tag="VLL06_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HSS30905.1"
                     /translation="MEAWRRILADSVVKPKDLADRLGVDAKEIESIVGDYPMRITPTV
                     MATIKERGDAIWKQVVPDIAELDDFHAEADPLEEDLMSPVPHLVHRYPDRVLLMVTNQ
                     CPIYCRFCTRKRLVGKPGFLKKGELDQAIAYLREHTEVRDVILSGGDPLLLPDRLLER
                     ILKALRTIPHLELIRLGSRVPGTLPERITPELCTIVKRYHPIYMNLHFNHPDELTPAV
                     KAACGMLADAGVPLGAQTVLLKGVNDDPDIMKRLMHQLLLARVKPYYLYQADLTKGTN
                     HFRTTVETGLNIIKSLQGHTSGMAVPHFVIDAPGGGGKIPLLPGDYMVHMDAEGVLLK
                     NYENKAFHYPQPPQGSARELPMVGAGPSLGSCSTGEHDDL"
     gene            3025..4176
                     /locus_tag="VLL06_07800"
     CDS             3025..4176
                     /locus_tag="VLL06_07800"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HSS30906.1"
                     /translation="MSTRTTQGILPYQQIKQLIASRAIHTDVPIEDRQIQPASLDLRL
                     GRKAYRLISSFLPELSEITSRLNILDFYQSDLVMYEMDLTEGAILEKGHVYLVPLLER
                     VTLPKTIRARTNPKSTTGRLDVFTRVVTDLNTGFDEIRAGYSGPLYLEIVPRSFTIKV
                     KTGYSLNQIRFVRGDAAVSDRSLQTLHKREPLLYHNLPTKHALDTRDFRTDRGLFLRI
                     DLKGDNQSGSPIIGYRAKKNSHVIDLSKIGHYAALDFWEPLYRHRQDSLLLEPEEFYI
                     LASKERIRVPPGYASEMVAFEAACGELRTHYAGFFDPGFGYGRGELHGTQVVLEVRPH
                     DVPFLIHDGQTFFKVVYDRMLARPSQLYGTALGSSYQRQGLTLSKHFKV"
     gene            4267..4656
                     /locus_tag="VLL06_07805"
     CDS             4267..4656
                     /locus_tag="VLL06_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30907.1"
                     /translation="MPPEPTATNEPKRPERDDDSGGDNNLRVAGMIVGTALIFIGFLD
                     IFLSISGGFEIDFIPFLIYFGGIAVWSNAVVENATLRYSIIGAALILAAVFFHFGEVL
                     FWHKQVVFWGTVVVVMYFMFNEPKKPT"
     gene            4699..5460
                     /locus_tag="VLL06_07810"
     CDS             4699..5460
                     /locus_tag="VLL06_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HSS30908.1"
                     /translation="MTQPSSQSSDPVHRSPRAALVIFAKAPIPGEVKTRLCPPLTPDE
                     AATLHGSFVLDMLERSKTAVAKLKLPLDRYLACMPSSTLVFFKIMEERQGVKLVDQVG
                     DDLGTRIQHTFETMFMKRYQRVFIVGTDVPSLPLDYYVQALALLEKNDLVLGPALDGG
                     YYLIGLKHMVPDLFVGIPWSTARVLEITQEKATTLGLKTALLPPWRDVDTIEDLHALI
                     ETSALDAKKPKNEQSFSGRTAGALQLIAKRLQSRI"
     gene            5489..6223
                     /locus_tag="VLL06_07815"
     CDS             5489..6223
                     /locus_tag="VLL06_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSS30909.1"
                     /translation="MNERVALITGGAKGIGRGIALDLAARHWKIAICYRTSEAEAQKT
                     SLAITERGGQALAIRCDVSNPVAAKSLVDQVERTWGKIDALINGAGPYHRINLFEETV
                     EGWNEMFDGNLHPIFYLAQAVAPGMKARKAGHIISFSMANADHMISQPDVTAHYIAKA
                     GILILTRTLAKLLAPHGITVNAVSPGFIDSGSAPPGELAGMTKRIPAGYVGTVDDTVA
                     AVRYLLSDEARYVNGANIQISGAWGI"
     gene            6356..6760
                     /locus_tag="VLL06_07820"
     CDS             6356..6760
                     /locus_tag="VLL06_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30910.1"
                     /translation="MRSEQERIAHGRFVKALQHEHLTCAQPGCGGAMDIADHTLHNAH
                     IKTYEAECERCHVKEQIAGIEQPTPPWDVASFTMMAELHLLHEQPICPFDDTPITFTS
                     MPNPRRKARYRLSCFYCGRHAEMNWPPPESKR"
     gene            complement(7114..7518)
                     /locus_tag="VLL06_07825"
     CDS             complement(7114..7518)
                     /locus_tag="VLL06_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30911.1"
                     /translation="MNRFGPRWLLLLFSLTTVTGCDFATDPATRLAFDLEAGTGRLGR
                     GAGATYSVQHKQPSKSGECVGPYKVQLDKVGALIIWCNDASGNTVSSHSTSYHARFVE
                     TPQTYIVEKPAGTALTIDLERRNDRAVITNVR"
     gene            complement(7802..8125)
                     /locus_tag="VLL06_07830"
     CDS             complement(7802..8125)
                     /locus_tag="VLL06_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HSS30912.1"
                     /translation="MADPIQEEINKEVAAHKILIYGKGTKQMPMCGFTRETIQFFDKY
                     GYPFELIDVLSQPAKREALAKMTNWPTLPKVFIDGQFYGDTDILDPMAAKGEIEPLLK
                     KAFGK"
     gene            complement(8137..8376)
                     /locus_tag="VLL06_07835"
     CDS             complement(8137..8376)
                     /locus_tag="VLL06_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HSS30913.1"
                     /translation="MITPDVLTEYVRKVMPDAAVTVMDRTGTMNHLKVVVISAAFAGK
                     NLLDRHRLVYQALDVPMKDGRIHALEITAQTTEEA"
     gene            8746..9180
                     /locus_tag="VLL06_07840"
     CDS             8746..9180
                     /locus_tag="VLL06_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS30914.1"
                     /translation="MSDFKSGFFIGGEACEGRVLVVDDEPDIRKVVKMTLQKAGYEVL
                     EAENGEKAIEVINYGENRLLLDVVICDIRMPKINGADAVAYFRSHYPRVPLIVLTGFP
                     DTDMATSFLRQGVVDYLVKPVEGEKLRAAVARALEQRQLAQL"
BASE COUNT         2119 a         2786 c         2470 g         1952 t
ORIGIN      
        1 cctcttgtac gctcctaaag cccgtgttac agtcctggag cctcgcatga gaggcgctca
       61 ttgacacgga tactgcctct gtctgatttg gctacctccg acctccttca tcgttgcgcg
      121 gtcatccgtg accagttgag ccgagctggg ctctttccat ctctatccga ccaggccccg
      181 atgtccggaa tggtcgctgc cgcgacaaca tggcgaattg cgccgactcc attccctctg
      241 acgaaagatc agttacattt tttccaccaa ctcggaccgc aactcttatc tttttatcga
      301 gccctgaacc gcctctacgc cgacagcata cgcggactcc aaccagcctg gatcgcggga
      361 tacctggacc aaggcaaacc ggaagcgttg ctcacataca gtcggatgaa gcggtttcgt
      421 gaccaacttc ccgcggtcat ccgaccggac atcattccga cccaggacgg catggtaatc
      481 accgaactcg actcagtccc aggaggaatc ggaatgaccg cgtgcctctc ggcgctctat
      541 accgatcggg aaaatgacca cctctcactt gtcggaggcc ccgagggaat ggtgcgagga
      601 tttgcggcga tgttgagaag ccagctcgtc cagcatacgg gatgcgtcgc cattctggta
      661 tcggaagaag ccaaagacta tcggccggaa atgacgtggc tcgcggccca gctccgaacc
      721 atcggcctcg acgcctactg cgtggaacct cgtcagattc gattcaccga ggaaggcctc
      781 cggatcacca gcgatgacat cgaccagccg gtagccttga tttatcggtt ctatgagtta
      841 ttcgatctgc tgaacattcc caaatccgaa ctcgtccaat atgcgctcaa gaaagaccgt
      901 gtcaccgtca caccaccgta taagccggca ctggaagaga agctggcctt cgccctcctg
      961 catcacccaa tgttgcggcc gttctgggag caggccttga gcagtgaggt gttcctgcat
     1021 ctgaccgcca tcatgccccg tacatggatt ctggatcctg cgccgatccc tccgtccgcc
     1081 acaattcccg gcttggtcct tggcggacgg gccgtgatga actggcgcga tctggctacg
     1141 gcaacccaga aagaacggca catggtcatc aagccatcgg gcttctctga actcgcctgg
     1201 ggcagtcgcg gcgtatcggt tggacacgat atgccgcaag gagaatgggc cggtgccatc
     1261 gacaatgcac tggctgcctt tccccataca ccctatatcc tgcaagaatt ccacaagggc
     1321 cgcctgtatg agctcgacta cttcgatccg gtcaccggtg agatgaaccg gatgtccggt
     1381 cgcgccaggc tctcccccta ttatttcgtg agtggcgaaa agaccgagct ggctggtatc
     1441 ctggctacca tctgcccggc ggataaaaaa gtgatccacg ggatgaaaga tgccgtcatg
     1501 gtaccctgtg cggttgctgg accatgagca aggtcgaaga tgaatgctga atgaatggca
     1561 cagtgtcgtt cggacaaatt accactcaac attgagcatt caccatttgt atctatggcg
     1621 atcaccctct atcacgtggc ctggtgtccc gactgtaagg aggtgcgcca gaaactggca
     1681 gacctccaca tggagtacga gcagatcgtc gtgcccgact ttcgcccgat gcgcaaagtg
     1741 gtacaagaag tctcaggcca atactatgtt cctgtcctca aggatgggga cctcgtgttg
     1801 acggaaaccg acgacatcct cgatcatctg gaaaagacgt acggtcagga gcggatcgcc
     1861 ggcagttaat tgccgccgat cattcgacag taaaggagac tgcatggaag catggagacg
     1921 catactcgcc gacagcgtgg tgaaaccaaa agacttggcg gaccggctcg gtgttgatgc
     1981 gaaagaaatc gaatccatcg tgggcgacta tccgatgcgc attaccccca cggtgatggc
     2041 gacgataaaa gaacggggcg atgccatctg gaaacaggtg gtcccggaca tcgcagagct
     2101 ggacgacttc cacgcggaag ctgatccgct cgaagaagac ctcatgagtc cggttccgca
     2161 ccttgtccat cgctacccgg accgtgtctt attaatggtg acgaaccagt gcccgatcta
     2221 ttgccggttt tgtacgagga aacggctggt tgggaaaccg gggttcctca agaaaggcga
     2281 actcgaccag gccatcgcct acttgcgcga gcataccgaa gtgcgcgacg tcattctctc
     2341 cggaggcgac cccctgctcc tgccggatcg tctattggag cggatcctga aggccctgcg
     2401 taccatcccg catctggaac tgattcgcct cggctcccgg gtgcccggca cgctaccgga
     2461 acgaatcacc ccggaactct gcacgatcgt caagcggtac caccctattt atatgaatct
     2521 ccacttcaat cacccggacg aactgactcc ggcagtcaaa gccgcctgcg ggatgttggc
     2581 cgatgccggc gtgccgctcg gcgcgcaaac cgtactgctc aaaggcgtca atgacgatcc
     2641 ggatatcatg aaacgtttaa tgcatcagct cctgctcgct cgcgtcaaac cgtactatct
     2701 ctaccaagcg gacctgacca agggcacgaa tcacttccgc acgacggtcg agacggggct
     2761 gaacatcatc aaatcgttgc agggtcacac aagtggaatg gccgtcccgc acttcgtgat
     2821 cgacgcgccg ggcggcggcg gcaagattcc cttactcccc ggtgactaca tggtgcacat
     2881 ggatgccgag ggggtcctct tgaagaacta tgagaacaag gcattccact acccgcaacc
     2941 gccgcaggga tccgcgcggg aactgccgat ggtcggcgca ggaccttcac tgggatcgtg
     3001 cagtaccgga gagcacgacg acctgtgagc acccgaacca cgcaggggat tttgccctac
     3061 caacagatca agcagttgat cgcgagccgc gccatccaca ccgacgtgcc gatcgaagac
     3121 cggcagatcc agccagccag tctcgatttg cgtctcgggc gcaaggccta tcggttgatc
     3181 agcagctttc tgccggagtt gtccgagatc acgagccggc tcaacatcct ggacttctac
     3241 cagtccgacc tcgtgatgta cgagatggac ctgaccgagg gggccattct cgaaaagggg
     3301 cacgtctatt tggtgccgct gctggaacgt gtgacattac cgaagaccat cagggcgcga
     3361 acgaatccta aaagtacgac cggacggctg gacgtcttta cccgcgtcgt aaccgatctc
     3421 aacacaggat tcgatgaaat ccgagccggc tattctggac ccctctatct cgaaattgtc
     3481 ccccgctcgt tcacgatcaa ggtgaagacc ggatactctc tcaatcaaat tcgatttgtg
     3541 cgcggcgatg ccgcggtgtc agaccggtcc cttcagacgc tccacaagcg agagcccttg
     3601 ctttaccaca acctccccac gaaacacgcg ctggacaccc gcgatttccg aacggatcgc
     3661 ggcctgtttc tccgcatcga tctgaaggga gacaatcaaa gtggctcgcc gattatcggc
     3721 tatcgggcga agaagaatag tcacgtcatc gatctgtcaa agatcggcca ttatgcggcg
     3781 ctcgattttt gggaacccct gtaccgtcac cgccaggaca gcctcctctt ggaaccggag
     3841 gagttttaca ttctcgcatc gaaagaacgg attcgcgttc ccccaggcta cgcatcagaa
     3901 atggtcgcct tcgaagcagc ctgtggcgaa ttacggaccc actacgccgg attcttcgat
     3961 ccgggattcg gatatggtcg cggcgagttg cacggcaccc aagtagtctt ggaggtccgc
     4021 ccccacgacg tgcccttcct catccatgat ggacaaactt tttttaaggt tgtatacgac
     4081 aggatgctcg cacgccccag tcagctctat ggcaccgcac tcggatcctc gtatcaacgc
     4141 caaggtctga ccctgagcaa acattttaag gtttagagcg catctcgtga ggcgtcgctc
     4201 gctcctgatc ctcacatttc acgcttcacc tatcacgtgt cacgaacgac gaggaaccag
     4261 ctgaccatgc cgccagaacc gacagcgacc aatgagccaa agcggcccga gcgagacgac
     4321 gactccgggg gcgacaacaa tctgcgtgtc gccgggatga tcgtgggcac cgccctcatt
     4381 ttcatcggct tcctcgacat ttttctctcg atcagcgggg gattcgaaat cgacttcatt
     4441 cccttcttga tctatttcgg tggaatcgcg gtgtggtcga atgcggtcgt cgaaaacgcg
     4501 accctccgct acagcatcat cggcgccgcc ctcatactcg ctgccgtctt cttccacttt
     4561 ggagaagtcc tcttctggca taagcaagtc gtgttttggg ggactgtggt agttgtcatg
     4621 tatttcatgt tcaacgaacc gaaaaagcct acctagcagg atgctgaaac cgcccggcag
     4681 cggacggctc gatgatccat gacacaacct tcctcccagt cgtcagatcc cgtccatcgg
     4741 tcacctcgcg ctgctctcgt gattttcgcc aaagctccga tccccggaga ggtcaaaacg
     4801 cggctctgtc cgccgctaac ccctgacgag gccgccacgc tgcacggcag tttcgtgctc
     4861 gacatgctgg aacgatcaaa aaccgcggtg gcaaagttga aattgccgct cgatcgctac
     4921 ctcgcctgta tgccatcctc aaccctcgtg ttttttaaga ttatggagga gcggcaaggc
     4981 gtgaaactgg tcgatcaagt cggagacgac ttgggcaccc gtatccagca cacatttgag
     5041 acaatgttca tgaaacgata tcagcgagtg ttcatcgtcg gtaccgacgt gccctcgctg
     5101 ccgctcgact actatgtaca agccctcgcc ctactcgaga agaatgatct tgtgttaggc
     5161 cctgccctcg atggcggcta ctatctgatc ggtctgaaac acatggttcc cgatcttttc
     5221 gtgggaatcc cctggtcgac cgctcgggtt cttgagataa cccaggaaaa agccacaacg
     5281 ctcggactca agaccgcact gttgccccca tggcgggatg tcgacaccat cgaagatctt
     5341 cacgccctca tcgaaaccag tgcactcgat gccaaaaaac cgaaaaacga gcaatcgttt
     5401 tcaggccgta cagctggagc acttcaactt atcgccaaac ggttacagtc acggatataa
     5461 taggtgcatc caaccgtagc agacctacat gaacgagcga gtggcactga tcacgggcgg
     5521 ggccaaagga attgggcgtg ggattgccct cgacctcgcg gcacgacatt ggaaaatcgc
     5581 tatctgctat cggaccagcg aagccgaggc ccagaagaca tccctcgcca tcacggaacg
     5641 aggcggccag gcgctggcga ttcgttgcga tgtgtctaac ccggttgcgg cgaagagcct
     5701 ggtggaccag gtcgaacgga cctggggaaa aatcgatgcg ctgattaacg gagccggccc
     5761 ctaccaccga atcaatcttt tcgaagaaac tgtcgagggc tggaacgaga tgttcgacgg
     5821 caacctccac ccaattttct atctcgcgca agccgtcgca cccggcatga aagcccgtaa
     5881 ggccggtcac atcatcagct tcagcatggc caatgccgac cacatgattt cccaaccaga
     5941 cgtcacagcc cattacattg cgaaagccgg gatcttgatt ctcacacgca cgctcgcgaa
     6001 gttgctggca ccccacggca ttaccgtcaa tgcggtttcg ccaggattca tcgattcagg
     6061 cagtgcgccg ccgggagaac tcgccggcat gaccaaacgc atcccggccg gctacgtcgg
     6121 caccgtcgac gatacggtgg cagccgtccg ctatctcctg agcgatgagg cccgctacgt
     6181 caatggcgca aacattcaaa tcagcggcgc ctgggggata tagagggtag cctgagtgcc
     6241 cttcttgctc gcagaacgcg cacactcaga agtatgctcg ttcgatgcgc gcagtgaagg
     6301 acactgcagg ccaccctcag tagaggagtg aggaagagga cctgagtgag ggcttatgag
     6361 gtcggaacag gagcggatag cgcatggccg gtttgtcaaa gctttacagc acgaacacct
     6421 gacctgtgcc caacctgggt gcggcggggc gatggatatc gccgatcaca ccctgcacaa
     6481 cgcccacatc aagacctacg aagcagagtg tgagcgctgc catgtgaagg agcagattgc
     6541 gggaatagaa cagcccaccc caccatggga cgtcgcctca ttcaccatga tggccgaact
     6601 gcatctcctt cacgaacaac ccatttgccc ctttgatgac acccccatca cctttacctc
     6661 catgccgaat ccccgacgca aagcccgcta ccgcctctcc tgcttctact gcggccggca
     6721 cgcagaaatg aattggccgc ctccggaatc caagaggtag agggatgcgg ggcaaggtgc
     6781 caacctcagt tgctcgcgca acgcgcggcc tccgaaggat gggaagggag cggccaggtg
     6841 cccttcttgc tcgcagaacg cgcacggtga aactgtgctc gttcgatgcg cgcagtaaag
     6901 ggcatcctcg gccactccct taacgagaga tgaagaaggt tggaaggccc tcgcccaggg
     6961 ctaatggcac actccggcgt gccattgggt gggcgggtaa aaaattgcgc gcggatgagg
     7021 tgagcatcct tgcagggtgt cgttccgaga gagaaaacac gagcaagctt agaggaagca
     7081 ctaaacattt actcgactgc ctgttttccc acgctaccgg acattggtga tcacagcccg
     7141 atcattgcgt cgttcgagat cgatagtcag ggccgtacca gccggctttt ccactatgta
     7201 tgtttgagga gtctcgacaa agcgcgcatg gtacgacgtg ctatggctgg atacggtatt
     7261 gccggaagca tcgttgcacc agataattaa tgctcccacc ttatcaagct gcactttata
     7321 cggaccgaca cattcaccag atttcgatgg ctgcttatgc tgaacactat acgtggcacc
     7381 agccccacga cccaagcggc ccgtaccggc ctcaagatcg aacgctagcc gcgtcgcagg
     7441 atcggtagca aaatcacagc ctgtcacagt agtgagggaa aataggagga gcaaccaacg
     7501 cggaccgaat ctattcatgc gctctcctgg tcatgctgca tgacaaataa catgggcgac
     7561 ggtgatgctc tcactgcgcg catgcaccga tcaccttctg aggtgagggg tgcgcgagca
     7621 taagagcatc gccgtcgcct cttttctctc tctccctcgc ccacccgctg cctgaacaaa
     7681 ataggaaggg cgtctgtcga actcttgatt gcgcgcgttc aacgagggcc ttctgaggcc
     7741 gcgcgttgcg cgagcaggag agtttggcag acgcccttcc tactctttct ttccgaccga
     7801 tctattttcc gaatgctttt ttcagcagcg gctctatctc gcctttggcc gccatcggat
     7861 ccagaatatc ggtatcgccg tagaactgcc cgtcgatgaa tactttggga agcgtcggcc
     7921 agttggtcat tttagcgagc gcctctcgct tggccggttg agacaggacg tcaatgagtt
     7981 cgaacgggta tccgtacttg tcgaagaatt ggatcgtctc cctcgtaaac ccgcacatcg
     8041 gcatctgttt cgtgcccttc ccataaatca aaatcttgtg cgcggcgact tccttgttaa
     8101 tttcttcttg aatgggatct gccatgggtc ggcctcctag gcttcttctg tggtttgagc
     8161 ggtaatttcc aaagcgtgaa tccgaccatc cttcatcggg acatccaacg cttgatagac
     8221 caggcgatgg cgatccagca aattcttccc tgcaaaggcc gctgaaatca ccaccacctt
     8281 gagatgattc atcgtaccgg tccggtccat gaccgtgacc gccgcgtccg gcatgacctt
     8341 cctcacatat tctgtcaaca catccggtgt aatcatggtc tctccaatct cgtccctgtc
     8401 agaatacctc agcgccagac tgaaaggcaa ttcggtatgg tgagaagcgg ctggttttcc
     8461 ttgtttattt ggtgtctttg attgaaccca acgaaccaga ggaactcgat caaccaggca
     8521 aaccaactca acctcctcac attttggtgg ttcatttcag cccatgatgg tggatatcaa
     8581 ccattgcatg cgtattccag tttccataaa acccgtcttt caagggaatc tgaactgctt
     8641 ttcccacaaa ggctgctggc atgcgcattg cgcttccttg caacaagaac atatgagccg
     8701 accaatatac acacccacat ctactgttca accaaggagg atgccatgag tgatttcaaa
     8761 tccggattct ttatcggagg agaagcctgc gaaggacggg tgcttgtggt agacgacgaa
     8821 cccgatattc gtaaagtcgt caagatgacg ctgcagaaag ccgggtatga agtccttgag
     8881 gcggaaaacg gtgagaaggc gatcgaagtc atcaattacg gcgagaaccg gctccttctc
     8941 gacgtcgtca tctgcgacat tcgtatgcca aaaatcaatg gagccgacgc cgtcgcgtac
     9001 ttcagaagcc actaccctcg cgtcccgctc atcgtgctga ccggcttccc ggataccgat
     9061 atggcgacat ccttcctccg gcaaggtgtt gtggactatc tcgtcaagcc ggtcgaagga
     9121 gagaaattga gagcggccgt cgcgcgggct ctcgagcagc gacaacttgc gcagttgtaa
     9181 cggtgagcct ctatcctctc tgggccaggg agccgcatgc ccgtcattgc tctcctcggc
     9241 cacagagagg accccgttgc cacagcaaag ccgttgacat catttcaaaa gacccggcga
     9301 gtgatggact actcgccatc attgctc
//
LOCUS       DATIPF010000314         2965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_60603, whole genome shotgun sequence.
ACCESSION   DATIPF010000314 DATIPF010000000
VERSION     DATIPF010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60603"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /locus_tag="VLL06_07845"
     CDS             complement(<1..1417)
                     /locus_tag="VLL06_07845"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HSS30915.1"
                     /translation="MNYSTRGCFPSLVVFILLGIILVLLPIGHQAFAQSEEDHPVFLH
                     ALDSLSGERMLADVTRLSDPSFNGRQAGTDDDLSSATWMADQFRKSNLTLAIVPLDRL
                     NILGRDGAPSTGYMSKPYQFNRIAPEPTLQLSSLESPENQTLGTDFLPVFDSPSAHVR
                     VPIVFVGYGIVDATSGINDYAGIDVNNCIVMFLRGKPDHFKGTTTHADKVRTARHKGA
                     VGYLTATGPVLSSYEMRRGVTGLPSAFYGLSPTPDNLPGAWISTSLADKIIAGGDNPN
                     QEHLRKLQEQLNRTTESRSFSTAYVGALEWESRQEQSFLVNVIAILPGNDPQLKHEAI
                     IIGAHRDHFGRQAGLLFPGADDNASGTAVMLEVARTLSTLSAHVNRPTRPGRLSETSG
                     EPKRTILFVSFSGEEQGLIGSRLYVDRPLVPMTMTKAMINIDHAGIGNGRLTVGVTGL
                     EKTIAADAGVSAAVADKLDLCG"
     gene            1499..>2965
                     /locus_tag="VLL06_07850"
     CDS             1499..>2965
                     /locus_tag="VLL06_07850"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSS30916.1"
                     /translation="MADYQDEETMTDLTLQADRFSNRLITEASPYLRQHAKNPVDWYP
                     WGEEALSRSKAEDKPILLSIGYSACHWCHVMAHESFENAEIAGLMNRDFINVKVDREE
                     RPDLDDIYQKSAQVFLGRGGGWPLTMFLTPDQEPFYGGTYFPPAPRYNLPGFPEVLRG
                     VVEAYRNHRDDVRKNVEKVKTGLLRVGTPQPSTDPLTDRLLDEAAKNLGQFFDPVHGG
                     FGEGPKFPTVPPLSLLLRQTARTKDISHQEHVLLQLRTMAAGGIYDHLGGGFHRYSVD
                     GQWLIPHFEKMLYDNAQLVRIYLDAWRLTKEPRFREVVEETLEYVRRELTNQDGAFFA
                     AQDADSEGHEGAYFVWEPGEIAAVLGIDMAEEFCRYYGVTESGNFEGKNVLNRLGGIQ
                     LGPEAQQDVASSLRPARMKLLVAREQRMMPQRDDNILTSWNGMMVSAYLDAYHAFGVP
                     AYLAAAEQALTFLLDYAVENGRIYRTVRGGKGRLNGYLD"
BASE COUNT          656 a          746 c          898 g          665 t
ORIGIN      
        1 aaccgcacag atccagtttg tccgcaactg ctgccgacac gcccgcgtct gccgctatgg
       61 ttttctccag tcccgttact ccaacagtga gtcgtccgtt gccgatcccg gcgtgatcga
      121 tgttaatcat cgccttggtc atggtcatgg gtacaagtgg gcggtcgaca tagagtcgag
      181 aacctatgag accttgttct tctccactga acgaaacaaa gagaatggtc cgtttcggct
      241 ctcccgaagt ttcagacaac ctgcccggtc ttgtgggacg attgacatgt gcgctgaggg
      301 tggagagagt acgggccacc tccaacatca cggcagtccc tgaggcattg tcatcggctc
      361 ctggaaacaa gagtcctgct tggcggccga aatgatctcg atgtgctccg atgatgatgg
      421 cttcatgctt caactgcgga tcattgcctg ggaggatggc gatgacattg accagaaagc
      481 tctgttcttg ccgtgactcc cattcaaggg ctccgacgta tgcggtgctg aaggaccgag
      541 actcggtggt tcggttgagc tgctcctgca attttcgaag gtgttcttga ttgggattgt
      601 cgccgccggc aataattttg tccgctagcg aggtgctaat ccaggcgccg ggaaggttgt
      661 cgggtgttgg agaaagtcca tagaaggcgc ttgggagtcc tgtgacacca cgtcgcatct
      721 cgtaggaact gaggactggt ccggttgcag tgagatatcc cactgctcct ttatggcgag
      781 ctgtccggac tttgtccgcg tgggtagttg ttcctttgaa gtgatcgggt tttcctctta
      841 ggaacatcac gatgcaatta ttcacgtcga ttccggcata gtcattgata cccgaggtgg
      901 catcgacaat cccatagcct acaaaaacga ttgggacacg cacatgcgcc gacggtgaat
      961 cgaacaccgg gagaaagtcg gttccgagcg tttggttctc agggctctcg agcgatgaaa
     1021 gttgcagggt cggttcaggg gctatcctgt taaattggta gggcttcgac atgtaaccgg
     1081 ttgatggggc accatctcta ccgagaatat tcaaccgatc caagggcaca attgccaagg
     1141 tcaggtttga ctttctgaat tgatcagcca tccacgtagc ggaactcaaa tcatcgtcgg
     1201 ttccggcttg ccgtccgttg aaggatggat cgctaagtct cgtcacgtcg gccagcatgc
     1261 gttcgcctga taggctatcg agcgcgtgca ggaagacggg gtggtcttcc tccgattgtg
     1321 caaaggcttg gtgccctatg ggaagcagta cgaggatgat tccaagcaga atgaacacaa
     1381 caagagaggg aaaacagccg cgcgtggagt agttcatgcg gtgtagcctt tcgaggtcgc
     1441 gcggaactat agcacagtct gacaggttcg ttgcagagcg gcagggagcc aaggtactat
     1501 ggcagattat caggacgaag agacgatgac agatctaacc ctacaagcgg accgtttttc
     1561 gaaccggctc atcacagaag ccagccccta tcttcggcag catgcgaaga atcctgtgga
     1621 ttggtatccc tggggcgaag aagccttgtc gcggtcaaaa gcagaggaca aaccgattct
     1681 gttgtcgatc ggctactcgg cctgtcactg gtgccatgtc atggcacatg aaagtttcga
     1741 aaatgccgaa attgccgggc tcatgaaccg ggacttcatc aacgtgaagg tcgatcggga
     1801 agagcgcccg gacctggacg atatctacca aaagtcggca caggtgtttc tggggcgggg
     1861 cggtgggtgg cccctgacga tgtttctgac gcctgaccag gaaccgtttt atgggggcac
     1921 gtattttcca ccggcgcccc gctacaacct gccgggattt cctgaggtct tgcgcggtgt
     1981 cgtcgaggcc taccggaatc atcgagacga tgtccgtaaa aatgtggaga aggtgaaaac
     2041 ggggttgctg cgagtcggaa caccgcagcc gtcgaccgat ccattgacgg atcgtttgct
     2101 cgacgaggcg gcaaagaatt tagggcagtt ttttgatccg gtccatggcg gattcggtga
     2161 agggccgaaa tttccgacgg tccccccgct cagcctgttg ctccgtcaga cggcaaggac
     2221 gaaagacatc tcccatcaag agcacgtact gttgcagtta agaacgatgg cagccggagg
     2281 gatttacgat catctcggcg gcggattcca ccgctattcg gtggatggcc agtggttgat
     2341 tccccatttt gaaaagatgc tctacgacaa cgcgcaactg gtgcggatct acctggatgc
     2401 ctggcgcctc accaaggagc ctcggtttcg tgaggtcgtg gaagagacct tggagtacgt
     2461 tcgtcgtgaa ctcacgaacc aggacggcgc attcttcgcg gcgcaggatg ccgatagtga
     2521 agggcatgaa ggagcctatt tcgtctggga acctggagag attgccgcgg tgctgggcat
     2581 cgacatggca gaggaattct gccggtacta tggggtgacg gagagcggca acttcgaggg
     2641 caaaaacgtg ttgaatcgac tcggtggaat tcaactcgga cccgaggcgc aacaagatgt
     2701 ggcgtcctcg ctacggcctg ccagaatgaa gttgcttgtc gcacgcgagc aacggatgat
     2761 gccgcagcgg gatgacaaca ttctcacgag ctggaacggg atgatggtgt ccgcctatct
     2821 cgatgcctat cacgcgttcg gagtccccgc gtatttggcg gcagcggaac aagcactcac
     2881 attcctgctg gactatgcgg ttgagaacgg acgcatctat cgtacggtta ggggcgggaa
     2941 ggggcgtttg aacggttatc tagac
//
LOCUS       DATIPF010000315         6788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1655515, whole genome shotgun sequence.
ACCESSION   DATIPF010000315 DATIPF010000000
VERSION     DATIPF010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655515"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VLL06_07855"
     CDS             complement(<1..213)
                     /locus_tag="VLL06_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30917.1"
                     /translation="MEVHLFALKLGFLAFWGLWFSVVFLTNLSDGCRLLGILPTTWKF
                     ASQNFQPVAKATGRYLAGAWLPVVLFS"
     gene            527..1291
                     /locus_tag="VLL06_07860"
     CDS             527..1291
                     /locus_tag="VLL06_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HSS30918.1"
                     /translation="MIESSFCFLPGVGRRTERRWWQRGIGTWEDFLSASHSIPGIGPS
                     RKALYDAALDQAKVHRAQEDSRYFATTLHTRDQWRLYEWLRPRAVYLDIETNSFGQIT
                     VVGLYGQGSFTSLVEGELLTRRRLCDELAQYDLLVTFNGTSFDLPVLLASIQGLPLDQ
                     PHIDLCLLSRQLGYRGGLKAIELQLGIHRRADLWGLSGADAGQLWNRWRHSRAEEARE
                     RLLAYNEADCVNLQPMADLFYCQMVQRHRSELGESR"
     gene            complement(1377..2123)
                     /locus_tag="VLL06_07865"
     CDS             complement(1377..2123)
                     /locus_tag="VLL06_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30919.1"
                     /translation="MLYGAWCLIVLASVQACSQPQQVNNPPCAASNAVIQVKPSADLS
                     LQFGNDAIQIALDTITRHVRQNDITSTTELTGLGTDAAVRTAEANGKNLKPLDRAVLA
                     AFLREDVVPAIKQSPTCGFMTVSTKRDVSIEDVYLENFGDKPIVKVKLSNTGQVGARF
                     INVVVRNMIDGMKPQSGQADMVLSPGQWRNVSNPQATLPMSDIGSGKKALIVIVQLSY
                     AKEAGSQPFVYQEEWKYDAPSQTFKQSPIK"
     gene            complement(2391..3650)
                     /locus_tag="VLL06_07870"
     CDS             complement(2391..3650)
                     /locus_tag="VLL06_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSS30920.1"
                     /translation="MASNKPDDRAKDLRHQEDRPVQLPSRTVPDLTSVRGEDIPILVQ
                     QLQVQHVQLEMQNEELRQAQETLAEACEHYTQLYDFSPAGYVTLTPEGTIEEANLRFC
                     ALLGLNRKFVVQRPFSDLVVTEDSGIFQRHCQEVLDSGTTRTCKLHFPRHTGTALVMH
                     VESIRVKDRDSSQRKIQSAMLDITGMERAEQAVQASQQKLQYTAAKLMTAQDEERRRI
                     ARDLHDDYCQRLSVIILEIGLMTRRHPEGWSDPTHLQPLKAMLVTLLSDLRDLSHDLH
                     PEQTASITLDDALRAYVADFTERTQIVATYHAPLQPVPLLPAISTCLYRVTQEGLTNI
                     RLHAKATRASVAMNGLPDAIELLIKDNGQGFEPQTVSGSHHLGLTSMRERVEQLHGAM
                     KITSRPDWGTTIVIQIPLRQGKDHTQD"
     gene            complement(3673..6495)
                     /locus_tag="VLL06_07875"
     CDS             complement(3673..6495)
                     /locus_tag="VLL06_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HSS30921.1"
                     /translation="MGIGSSAGGIEALEEFFRHLPANPGAAFVVVSHQHAGHSSLLPE
                     ILRRWTALPVFEASDGLPVEPNTIYLPPPGSRLAILHAVLHVMDGGDGVRPLLPIDYF
                     LCSLAEDQQEKAVGIILSGTGTDGSVGLKAIRAESGMTMAQEPRSAKFAGMPQNAITL
                     GVVDMIRPPAELGVQLSNFVQHRTVLQPGQVVDGDGKRDLNKILVYLRDRIGNDFSLY
                     KSNTTLRRIERRMNLHQIATLPHYAQFLQSDPIEVHALFSDLLIGVTSFFRDPSAYDI
                     LMQVGLPQLLDKKPDGATVRIWVPACSTGEEAYTLAMLLQEFRSQQKRKFTIQMFATD
                     IDGEAIDKARHGLYPEGIANQISPERLKSFFVKEDSHYRVKSELRDCVTFATHNLLKD
                     APFIKLDLLSCRNFLIYVRPETQHGLIPLFHYALKPMGLLMLGAAETVDGFTNLFTAL
                     DRKARIFTRDVGPASLPIREMRLAPTGDHVTPESIPGRHRVPLLVESIQTYLLDRFVP
                     SAVFVNRQGQVVYIQGRTGDYLEPGPGAANHQVTDMVRPELRPDLLAALQLAHKQGGT
                     VVRKGVRLQTPGSVRRVTFTVTPLSQPGPLDGLMLVVFDTESARAVKLSTTLRKVRPL
                     SKGRGRSLELEYAQQRLHRTNTDLQVSNEEFKSSNEELQSTNEELQSTNEELETTKEE
                     LQSLNEELITVNAQLQNKFEEAANANDDLRNLVNSSEVATIFLDGELCIKRFTPGASR
                     VSKVIATDIGRPFGDIVSTVRYDGLLDDARTVLQTLVFTEREVQAEGGNWYLLRMVPY
                     RTSKNLIDGVVLTYLDITAYKQATHRAESIVDGVPTPLIVLDTDLRVVMANRAFYQVF
                     ARERREVERHPLDRILEGRFNHPALRTAVEKRFIDATGVDSIELELESLPVGDGRWRA
                     SINRLIPAGDEAPLMLLALQHGPNTR"
     gene            complement(6566..>6788)
                     /locus_tag="VLL06_07880"
     CDS             complement(6566..>6788)
                     /locus_tag="VLL06_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30922.1"
                     /translation="DLNQAIQWVEQSAQGRYFGRSYRGGGGVALPYRDSLSCFMMTLG
                     NGGNDPTQKIGQTDSETAAGRCNPSARAS"
BASE COUNT         1447 a         1888 c         1919 g         1534 t
ORIGIN      
        1 ggaaaacaaa acaacaggca accaggcacc ggccagatat ctgccagtcg ctttggcgac
       61 cggttgaaaa ttctgagagg caaacttcca tgtggtggga aggatgccca aaagccgaca
      121 cccatcagag agatttgtca aaaataccac agaaaaccac aggccccaga acgccagaaa
      181 gccaagttta agtgcaaaca gatgaacctc catggcgaat gtccggaata tagccggctt
      241 gggtatcaat ggacatcttt gacctcatct tgccgtcaaa acctgcagca aggtctaatt
      301 ctggcctcgg gaatggatca ggtctcctgg gaaagagccc agggctgagt cagcgtgaaa
      361 gggcacacga gcaggctggg gcagtacagg cgaaaagcat tgacagagat gccatagcgg
      421 aaagccggct agccatatat atctatataa tggtgaataa ccgttcagaa gggaatgcac
      481 cattgaccat tcaggtcact tcgcgattac ctcaaggctg tccaacatga ttgagtcgtc
      541 cttttgtttc ttacccggag tgggccggag aactgaacga cgctggtggc aacggggcat
      601 tgggacttgg gaggattttc tgtctgcttc tcactccatc ccaggtatag ggccctcccg
      661 gaaggccctc tatgatgccg cactggatca agcgaaggtc catcgagccc aggaggattc
      721 gcggtatttc gccactactc tgcacactcg cgatcaatgg agactctatg aatggctccg
      781 cccccgggca gtgtatctgg acatcgagac gaattcgttt ggacagatca ccgttgtggg
      841 actctatgga cagggcagct tcacatcgct tgtggaggga gaattactca cccgacgacg
      901 gctctgtgat gagctggccc agtatgatct actcgtgacg ttcaatggga cgtcattcga
      961 tcttcccgtc cttctcgcaa gcattcaggg gctcccgctg gaccaacctc atatcgactt
     1021 gtgcctgctt agtcggcagc tcggttatcg cggtggtctc aaagctatcg aacttcagtt
     1081 gggcatccac cgtcgcgctg atttgtgggg cctgagtggc gccgatgcgg gccagctctg
     1141 gaatcggtgg cgacatagtc gggctgagga agcgagagag cggctgttgg cgtataacga
     1201 ggctgactgt gtgaatttac aacccatggc ggatctgttc tattgccaga tggtccaacg
     1261 gcatcggagt gaactcgggg aaagccggtg atgtcagaag ctctcgaaat gaggcctcat
     1321 gggactggtg tgtcctggta gcagcgaggt ggcagagaac taggagattg cggaatctat
     1381 tttatcggag actgcttaaa ggtttgagac ggagcatcgt atttccactc ctcctgatac
     1441 acgaagggtt gactcccagc ttctttggca taactgagtt gaacaatgac gatcagcgcc
     1501 ttctttccag atccaatatc agacattggc aatgtcgcct gcgggttact gacattgcgc
     1561 cattggcctg gacttaagac catatccgcc tgaccacttt ggggtttcat gccgtcaatc
     1621 atgttccgga caaccacgtt gataaagcga gcccccactt gtccagtgtt ggaaagtttg
     1681 actttgacta ttggcttgtc tccaaagttt tcgagatata catcttcgat actgacatct
     1741 ctcttagtcg acaccgtcat gaagccgcat gtgggactct gtttaatcgc gggaacaaca
     1801 tcttcgcgca aaaatgctgc caaaactgcc ctgtctagtg gtttcagatt tttaccgttg
     1861 gcttctgcag tacgaacagc cgcatctgtt cccaaacctg tcagttcagt cgtcgatgta
     1921 atgtcattct ggcgcacatg tcgtgtaatg gtgtctaaag caatttggat tgcatcattc
     1981 ccaaactgga gggatagatc ggcactcggt ttcacttgaa tgactgcatt ggacgctgca
     2041 catggtgggt tattcacctg ctgcggttga ctacaggctt gtacggaggc cagaacgatt
     2101 aaacaccagg caccatacaa cactcgtccc gttctcattg tgataggcct ccaatctata
     2161 caaggcggag aaattctatg tcattattca atgcggtcgt gaaagatcaa ctatgaaaaa
     2221 cgccagacat aggccgagca gccacagcga cctgcagtat gtgagagcgg gctgattaga
     2281 ccagatggga cacgatagtt ctgccaccgg gatggtacga gcggaatggc acagactgtt
     2341 ggctcctttc gaagtcagcg cggcgctccc tcctttatcg tgtcaaacct tcagtcttga
     2401 gtatggtcct tcccttggcg caagggaatt tgaatgacta tggtcgtgcc ccagtcaggt
     2461 cggctcgtaa tcttcatggc cccatgtaat tgttcgactc gctcccgcat gctggtcagt
     2521 ccaagatgat gtgaaccgga aaccgtttgc ggctcgaaac cctgtccatt gtccttgatg
     2581 agcaactcga tcgcatcggg caagccattc attgcgacgc tggcccgcgt ggccttggca
     2641 tgcaaccgga tgttggtgag cccttcctgg gtcacgcggt agaggcaggt actaatggcg
     2701 ggaagcagag gtaccggctg gagaggggca tggtaggtgg ccacaatctg tgttcgctcg
     2761 gtaaagtccg cgacataggc ccgcaaggca tcatccaagg tgatggatgc agtctgttcg
     2821 gggtgtaagt catgtgagag atcacggaga tccgacaaca gggtgaccaa catcgccttt
     2881 aagggttgca ggtgcgtggg gtcgctccag ccttcaggat gccgcctggt catgaggccg
     2941 atctctaaga tgatgactga gagtcgttga caataatcgt catgcagatc tcgggcaatc
     3001 cgccggcgtt cttcgtcctg cgccgtcatc agcttggcgg ccgtatactg caatttttgc
     3061 tggctggcct gcaccgcctg ctccgcccgc tccatgccgg taatgtccaa catggcggat
     3121 tgaattttcc gctgggaact gtcgcgatcc ttcactctga tgctttcgac atgcatcact
     3181 agggcagtgc cggtatgtcg cgggaagtgc agcttacagg tgcgtgtcgt cccactgtcc
     3241 aggacctcct gacagtgccg ctggaagatc cctgaatcct cggtcaccac caaatcggag
     3301 aatggccgtt gcaccacgaa tttccgattc aggcccagta aggcgcagaa tcgcaggttg
     3361 gcctcttcga tagtcccctc gggggtgagc gtcacatagc cggcaggaga gaagtcgtag
     3421 agctgcgtat agtgttcgca ggcctccgcg agcgtctctt gcgcttgacg gagctcttca
     3481 ttttgcattt ccagttggac gtgctgcact tgtagctgct gaaccaggat gggaatatct
     3541 tcgccccgca ccgaagtcag atcgggaaca gtccgtgacg gcagctgcac tggcctgtct
     3601 tcctgatggc gcaggtcctt ggcacggtcg tctggtttat ttgatgccat aggagcctcc
     3661 cttcgctgtc aattaccgtg tgttgggtcc atgctggagc gcgaggagca tcagcggcgc
     3721 ctcgtcgccc gctggtataa ggcggttgat actcgcccgc caccggccgt ccccgaccgg
     3781 gagggattcg agctccagtt cgatgctgtc cactcctgtc gcgtcgatga aacgcttctc
     3841 tacggcagtt cgtaacgccg gatgattgaa ccggccttcc agaatccggt caaggggatg
     3901 gcgctcaacc tcgcgtcgtt cgcgtgcgaa tacctgatag aacgctcgat tggccatgac
     3961 cacccggaga tcagtatcca gcacgatcaa gggggtcggc accccatcca caatggactc
     4021 cgcacggtgg gtcgcctgtt tataggccgt gatgtccagg tacgtaagca cgaccccatc
     4081 gatgaggttc tttgaggtgc gatagggtac cattcgcaag aggtaccaat tcccgccctc
     4141 ggcttgcact tcccgctccg tgaagacgag ggtctgcagc acggtgcgcg cgtcgtccag
     4201 gagcccgtca tagcgcacgg tggagacgat gtccccaaac ggacgtccga tatcggtcgc
     4261 aatgactttg ctcacgcgcg acgcgcccgg tgtaaaccgc ttgatacaga gctcgccatc
     4321 caaaaagatc gtggcgacct cgctgctatt cacgagattc ctcaggtcat cgttcgcatt
     4381 cgctgcttct tcaaacttgt tctgcaattg cgcattgacc gtgatcagtt cctcgttgag
     4441 cgattggagt tcttccttcg tggtttccaa ttcctcattg gtgctctgga gctcctcatt
     4501 cgtcgactgc aattcttcgt tggaggattt gaattcttcg ttggacactt gcagatcggt
     4561 attcgtccga tggagacgct gctgcgcgta ttcgagctcc aggctcctgc cccgcccctt
     4621 gctcaacggc cgcaccttcc gcagcgtggt tgacaacttc acggctcggg cggactcggt
     4681 gtcaaagacg accagcatga gtccgtccaa gggacccggc tgcgagagtg gtgtcacggt
     4741 gaacgtcacc cgccgcacac tcccaggcgt ctggagccgc acgcccttgc gcaccacggt
     4801 tccgccctgt ttgtgggcga gctgcaacgc cgccagcagg tctggtcgca attccggtcg
     4861 gaccatgtca gtcacttggt gattggccgc accaggccct ggttcgagat agtcacctgt
     4921 tcggccctga atgtagacta cttgtccctg ccgattgaca aacactgcgg agggcacaaa
     4981 tcggtcgagt aagtacgtct gaatggactc gacgagcagc ggcacccgat gtcgccccgg
     5041 tatagattcc ggcgtcacgt gatcgccggt cggcgccagc cgcatctctc gtatcgggag
     5101 cgaggcagga cccacgtctc tggtaaatat tcgtgctttc ctgtctaggg ccgtaaacaa
     5161 attggtgaac ccatcgaccg tttcggccgc gccgagcatc aacagcccca tgggcttcag
     5221 cgcatagtga aacagcggga tcagtccgtg ctgggtctcc ggacgcacat aaatgaggaa
     5281 attgcgacac gataggagat ccaacttgat gaagggcgca tctttcagca ggttatgggt
     5341 ggcaaacgtc acgcaatcgc gcagctcact tttcacccgg tagtggctgt cttccttcac
     5401 gaagaacgat ttcagtcgct ccgggcttat ctggttcgca atgccttcgg gatagaggcc
     5461 atgccgcgcc ttgtcaatcg cctctccatc gatgtcggtc gcaaacattt ggatcgtgaa
     5521 cttcctcttc tgctgactcc ggaactcctg cagcagcatc gcgagggtgt acgcttcttc
     5581 tccagtagaa caggccggca cccatatacg caccgtagcc ccgtccggct tcttgtccaa
     5641 gagctggggg agacccacct gcatgaggat gtcataggcc gaggggtccc gaaaaaacga
     5701 ggtcacccca atcaggaggt cgctaaacag ggcatggact tcgatgggat cagattggag
     5761 aaactgcgcg tagtgcggca gcgtcgcaat ctgatggagg ttcatccgac gctcgatacg
     5821 ccgcagcgtg gtgttggatt tatagagcga gaagtcattc ccgatgcgat cacgcagata
     5881 caccagaatc ttattcaggt ctcgcttacc gtcgccgtca acgacctgcc ccggctggag
     5941 aacggtgcga tgctgaacaa agttgctgag ctgaactcct aactccgccg gcgggcggat
     6001 catgtcgacc accccgagag taatcgcatt ttgaggcatg cccgcaaact tcgccgaccg
     6061 tggctcttgg gccatggtca tccccgattc tgctcgtatc gctttgagcc cgacggagcc
     6121 gtccgtgccc gtgcccgaga ggatgatgcc gaccgccttc tcctgttggt cttcggccag
     6181 cgagcacaag aaataatcga tcggtagcag cggacgcacg ccgtcgcctc cgtccatcac
     6241 atgcagcacg gcatggagga tggccaaacg gctgccgggt ggaggcaggt agatggtgtt
     6301 cggttccacc gggaggccgt cagaggcttc gaacaccggt aaggccgtcc agcgccgtaa
     6361 tatttccggc agcaggctgc tatggccggc atgctgatgt gagaccacca cgaaggcggc
     6421 accgggattg gcaggcagat ggcggaagaa ttcttcgagt gcttcaatgc ccccagccga
     6481 cgacccgatg cccacgatag gaaacgtcgc gtgtgtggag ggctccctca cgggatggtc
     6541 tggcacgaga cggtctaacg gggaattacg aggcgcgggc gctagggttg caacgaccgg
     6601 cggccgtttc cgagtccgtt tgaccaattt tttgggttgg gtcattccca ccattcccca
     6661 aggtcatcat gaaacacgat aggctatctc ggtagggcaa tgcaacccca cctccgcctc
     6721 gatacgatct cccaaagtaa cggccctggg cactctgttc cacccactgg atggcttgat
     6781 tcagatcc
//
LOCUS       DATIPF010000316         5443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1738581, whole genome shotgun sequence.
ACCESSION   DATIPF010000316 DATIPF010000000
VERSION     DATIPF010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738581"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..423)
                     /locus_tag="VLL06_07885"
     CDS             complement(145..423)
                     /locus_tag="VLL06_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HSS30923.1"
                     /translation="MYNVAQIIDEKCVAKKGCRLCIMYCPEANCLDLNPTKMVAEIII
                     DRCKGCELCVVVCDAAKHYAITMQAVSATGQLMTQKGESAALGQAYQG"
     gene            complement(445..1152)
                     /locus_tag="VLL06_07890"
     CDS             complement(445..1152)
                     /locus_tag="VLL06_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HSS30924.1"
                     /translation="MIKKRLNVRMSGLGGQGAVTAAHVLAMAANRDGKFSISNPFFGA
                     EKRMAPAESYCRIGIERIYDRGELVFPEVIEVFHPQVITMGKSYTMPFYSGVKEGGLV
                     IINSAQPLLSEEDIQRLKDLNVALFYIAGTELAIEVAGTELSTNMSMLGSVAGITKCV
                     SMEALDLALQERFGKKFVASGGTASLDEAIKKKFAKKEMLLAKNLATVKAAYDIASEW
                     AEKNQYELRVGNPAVAA"
     gene            complement(1177..2082)
                     /locus_tag="VLL06_07895"
     CDS             complement(1177..2082)
                     /locus_tag="VLL06_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSS30925.1"
                     /translation="MSKERIKISEDLYDIMPSDYQDLVRSATYGKEDRGWKDIGNNKE
                     LIEQHSLCAGCPESMAFRYILASLPNPEDTVMVGSTGCTSLVFPMVAVHNIHSLFGNQ
                     NAIASGLKRALSVRFPGRTKDVVVLAGDGATVDIGLDMTLQAWFRQEKFTTICFDNEL
                     YANTGGQESGLMQKGFVAKMAPVGKLFDKVRLPEIARESGCHYVISCTVSKPSLVEKV
                     VRNAVIIAREIGPTYLQLYTPCILEIGKNSMEGLQEMRDSEKPTERFAYKEYISEPAK
                     QLLAEVAAKDKEKKAAAKQLASQGA"
     gene            complement(2144..3355)
                     /locus_tag="VLL06_07900"
     CDS             complement(2144..3355)
                     /locus_tag="VLL06_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HSS30926.1"
                     /translation="MADTHSVIGTKNKKGQTFTETWTMMHDAPRTPSFFTGSEVIKEA
                     LRRASCDVMIAYPITPQSEAAALIGELFAEGYIGDYFRGESEFAVMSQCAGAAFGGAR
                     VFTTTAGPGTMRAMENFPMWAGARLPIQMIVTCRGINSPLSIQPDTLEIAYLLNTGML
                     VWHAETAQDFFDWILKGFMVSEEPDVHLPLALCCDGFFVTHTKDVVNLTPADMCLPPY
                     DPYRSPVPCMDMECPPVRMMRDPFVMKSNYISYATHASWQQEVWAAVERSRKHTIKWI
                     NGLIETENTDAEVLIVSSGTAVSQGREAIRLLEDEGIRCGLVKIKALRPWPGEEIREA
                     TKNAKHIIVPEFNVTGWLAKEIKATIPNSERVNAGPHVCGGMTMPPEIIVSEIKTLLG
                     MRTMSLAGRGS"
     gene            complement(3396..4031)
                     /locus_tag="VLL06_07905"
     CDS             complement(3396..4031)
                     /locus_tag="VLL06_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HSS30927.1"
                     /translation="MTQYRVLPGPEHFLPPSAASMGIRLPNPGEAHINGVIVPEEKAY
                     EEAAKQFLMAKVPTLFPGPLVLWAWNEKAAKKATAIRHLYNTLKECVQAGQTPMLIPM
                     PDYRPKYPKINPEVEINPNHPNLTIWHNKIDCCMFIGVHCHQANLSLKIIRGGTSCYT
                     IAMCAQAGHEDAMLSFRDASVEKIMKLAEWVKKLKGTVQPRLTSAKSGASN"
     gene            complement(4375..>5443)
                     /locus_tag="VLL06_07910"
     CDS             complement(4375..>5443)
                     /locus_tag="VLL06_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30928.1"
                     /translation="LESTFHDVEKESGGRNTQTSSLEIRTIAAAVTETEILESRRSFE
                     LDRPVFFVRIRATINMSSLAEAIRRLQSDQQLAQHYRQLQQENHQLRSQLQELQQQPI
                     GVRMLAIDPNGKSASHQRARAMLETALQTSNLRDKIQLSSDAATLDDRYVDPLIVRGQ
                     TYLRLVSLAFSQRVQASDYADYLDKAKTDFDHAITLDSKNLWAWVGKGDVLKWLKQLD
                     EAAIAYEQVLELDPFADVARLRLIDIYTTQARRQAGTRSWHQALATLKKILNGQTPES
                     WIPYQKEAYVLRSEIYLKMNQPEQAMEDLSTIIRVDPNNAAALVIRAKLYRERLQGRL
                     AKDDLERACVLGSIPACEQLP"
BASE COUNT         1128 a         1458 c         1491 g         1366 t
ORIGIN      
        1 ggatcaggcc agcaatctct cggacgagct catcaatgac agtatcttcg gtttccattt
       61 ttccctccac agcaggacca acaaaaaacc ccatagcgca ggcgccatgg ggtcttgcgc
      121 gggagacccg cttacacgaa acggttatcc ttggtaagcc tgcccaaggg ctgccgattc
      181 acccttttgg gtcattagct gcccggtggc actcactgct tgcatggtga tggcataatg
      241 cttggccgcg tcacagacga ccacgcagag ctcacaacct ttacagcggt caatgattat
      301 ttcggctacc atcttggttg gattgaggtc gaggcagttg gcctcagggc aatacataat
      361 gcagagccgg caccccttct tggccacgca tttctcgtcg atgatttgcg ctacgttata
      421 catgcaaact ggttccttct ctcgttacgc cgcaacggca ggattgccga cccgcagctc
      481 atactgattc ttctctgccc attcactggc gatgtcgtag gccgctttca ccgttgccag
      541 gttcttggct aacaacattt ccttcttggc gaatttcttc ttgatggcct catccaggga
      601 ggcggttcca ccggaggcga cgaacttctt cccaaaccgt tcctgtaagg cgaggtccaa
      661 ggcctccatt gaaacgcatt tggtaatgcc cgcgaccgat ccgagcatcg acatattcgt
      721 cgataactcg gtgccggcaa cttcgatggc aagctcagtg ccggcaatat aaaatagggc
      781 gacgtttaag tcctttaacc gctgaatatc ttcctcagag agaagaggct gggcagagtt
      841 gatgataacg aggccgcctt ccttcacgcc ggagtagaag ggcatggtat agctcttccc
      901 catcgtgatg acttggggat gaaagacctc gatgacttcg ggaaatacca actcgccacg
      961 gtcatagatc cgttcgatgc cgatccgaca atagctctcc gccggcgcca tacgtttctc
     1021 ggcgccgaag aagggattag agatcgagaa cttgccgtcg cggttggcag ccatggcaag
     1081 gacatgcgcg gcggtgacgg cgccctgccc gcctaatcct gacatcctga cattcagtct
     1141 tttcttgatc atggtcgtct ccccttcaga atcggattag gccccctgag aagcgagctg
     1201 ttttgcagcg gccttctttt ccttgtcttt ggcggctacc tcagccagga gctgcttggc
     1261 tggttcgctg atgtactctt tgtaggcgaa ccgctcggtc ggtttttcag aatcccgcat
     1321 ttcctggagc ccctccatgc tgttctttcc gatttcgaga atgcaaggcg tataaagctg
     1381 gaggtaagtc gggccgattt cgcgcgcaat tatgacagcg ttccgcacca ctttctcaac
     1441 gagcgaaggt ttactgaccg tacatgagat gacgtaatgg cagcctgact cccgcgcgat
     1501 ttccggtaga cggaccttgt cgaatagctt cccaaccggc gccatcttgg ctacgaaccc
     1561 cttctgcatc aggccgctct cctggccacc ggtgttggcg tacagttcgt tatcgaaaca
     1621 aattgtggtg aacttctcct ggcggaacca ggcttgaagt gtcatgtcga ggccgatatc
     1681 gacggtggcg ccgtcgccgg caagcacgac cacgtctttc gtccggcctg gaaagcggac
     1741 gcttaatgcg cgcttcaagc cggaagcgat ggcgttttgg ttaccaaaga gggagtgaat
     1801 gttatggacg gccaccatcg ggaataccag gctggtgcaa ccggtggaac cgaccatgac
     1861 ggtgtcttcg gggtttggca atgaggccag gatgtatcgg aaggccatgg attccgggca
     1921 gccggcgcag agggagtgct gctcgatcag ttccttgttg ttgccgatat ccttccaccc
     1981 ccggtcttcc ttaccatagg tcgcgctcct gaccaggtct tggtagtcag acggcatgat
     2041 gtcgtatagg tcttccgaaa tcttgatacg ttccttgctc atgatggcct cctcagtgat
     2101 gcgtcttatt tcagtgcgtt cgattcttgc tgctcaggtg cgatcaactc ccacgaccgg
     2161 ccaacgacat ggtgcgcatg ccgagcaggg tcttgatttc tgagacaata atttcgggtg
     2221 gcatcgtcat gcctccgcag acatgcgggc cggcattcac acgctcgctg tttgggatgg
     2281 tggctttaat ttcctttgcc aaccacccgg tcacgttaaa ttccgggacg atgatgtgct
     2341 tggcgttctt tgtggcttca cggatttcct cacctggcca aggccgcaag gccttgatct
     2401 tgaccagacc acaacggatg ccttcgtcct caaggagccg gatggcttca cggccctgcg
     2461 acaccgctgt accggaggag acaatgagga cctcggcatc cgtattttct gtctcgatca
     2521 atccgtttat ccacttgatc gtgtgcttac gagatcgctc cactgccgcc cagacttctt
     2581 gctgccagga cgcgtgggtg gcatagctga tgtagttgct tttcatcaca aatggatcgc
     2641 gcatcatccg gaccggtgga cattccatgt ccatacaggg tacgggtgag cggtaagggt
     2701 catagggtgg aagacacatg tcggcaggag tgaggttgac gacatccttg gtgtgggtga
     2761 caaagaatcc atcgcaacac agcgccaagg ggaggtgcac atcaggctct tccgacacca
     2821 taaacccctt gaggatccaa tcgaagaagt cttgtgcggt ttctgcatgc cacacgagca
     2881 tgccggtatt cagaagatat gcaatctcta gcgtgtcagg ctggatcgac agcggcgagt
     2941 tgatgccacg gcaggtgacg atcatttgaa tcggcaaccg tgcgccggcc cacattggaa
     3001 aattctccat tgcgcgcatc gtgccgggtc ctgcggtcgt cgtaaagacg cgagctccgc
     3061 cgaaggctgc tcccgcgcat tgcgacatga ccgcgaattc actttcaccg cggaagtagt
     3121 cgccgatgta cccttcggca aagagttcgc cgatcaacgc ggccgcttcg ctttgtggtg
     3181 taatcgggta ggcaatcata acgtcacagc tggcccgccg cagtgcttcc ttgatgacct
     3241 cactgcctgt aaagaacgac ggcgtacggg gcgcgtcgtg catcattgtc caggtctctg
     3301 taaaggtctg tcctttttta ttttttgtgc cgatgactga gtgtgtatct gccatgggtc
     3361 tggctccttt cactgtcttc agaacctcaa acagcttagt ttgaagctcc gctcttggct
     3421 gacgtcagcc gtggttggac tgtccctttc aatttcttca cccactctgc caatttcatg
     3481 attttttcga ccgatgcatc gcgaaatgac agcatcgcat cttcatggcc ggcttgagcg
     3541 cacatcgcaa tcgtgtagca ggaggtgcca cctcgaatga ttttgagcga caaattcgct
     3601 tgatggcagt ggactccgat gaacatgcag caatcgattt tattatgcca gatggtcaga
     3661 ttcgggtggt tgggattgat ctcgacttca ggattgattt tcgggtattt ggggcggtag
     3721 tccggcattg ggatcaacat gggtgtttgt cccgcttgca cacattcctt gagcgtattg
     3781 tacaaatggc ggatggccgt ggccttcttg gccgccttct cgttccaggc ccataggacc
     3841 aaggggcccg ggaagagggt tggcaccttg gccatgagaa attgttttgc tgcttcctcg
     3901 tatgcctttt cctctgggac gatgacaccg ttgatatgag cttccccagg gttcggcaaa
     3961 cgaattccca tgctggcagc tgatggagga aggaaatgtt ctgggcctgg aaggacacga
     4021 tactgagtca ttacggctta ttctccggca aggggttaaa gaacgagaac cacaagtttt
     4081 ccaatgatct cagccatcct cactctaagc tttttccgtg gcccctgcaa tatcgcagaa
     4141 ggcccaccgc tgagaaattt tgggccatcg attgggagag actaggcctt ttgtcctaag
     4201 atggagagtt ttgccaaagg tagccactag cgctcgtcta gctcgcattt taggagtgcg
     4261 cattataggg cccgcccatc cagtctgtca aaccaaaggg gaggattcaa actcccatgg
     4321 actggctggc ccacacttga gatatgagca tcaaaccaat aggttgtgcg gtgtttatgg
     4381 taattgctca caggctggaa tggatccgag aacgcaggct cgctcaaggt catccttggc
     4441 gagccgcccc tgtaaacgtt ctcgatagag ctttgctctg atcaccagtg cggcagcatt
     4501 gttggggtcg acccgaataa tagtagacaa atcttccatc gcctgctccg gctgattcat
     4561 cttgagatag atttcactcc gcagtacata cgcctctttt tgatacggga tccaactttc
     4621 aggtgtttga ccattgagaa ttttcttcag cgtcgccaag gcctgatgcc aagatctggt
     4681 gcccgcctga cgacgggctt gcgtcgtgta tatatcaatg aggcgcagac gagcgacatc
     4741 ggcaaacgga tcgagctcaa ggacttgttc gtaggcaatg gcagcctcat ccagctgttt
     4801 cagccatttg agcacatccc ctttcccaac ccatgcccag agattcttgg agtcgagtgt
     4861 gatggcatga tcaaagtctg tcttcgcttt gtcgagatag tctgcatagt ccgatgcctg
     4921 gactcgttgg gaaaaagcca gtgagacgag tcgcagatag gtttgccctc gcacgattag
     4981 tggatcgacg tagcgatcgt caagggtggc ggcgtcggaa ctgagctgga tcttgtcccg
     5041 tagattcgag gtctggaggg cagtctcgag catcgcgcgg gcccgttggt gtgaggcaga
     5101 ctttccattc ggatcgattg ccagcattcg gacaccgatc ggttgttgtt gaagctcttg
     5161 cagttgcgat cgcaattggt gattctcttg ctggagctgg cgatagtgct gcgcgagttg
     5221 ctggtcggat tgaagtcggc gaatcgcttc cgccagactg ctcatattaa tcgtggcacg
     5281 aatgcgtacg aagaagaccg gacgatcgag ctcgaaggag cgcctggatt cgaggatttc
     5341 cgtttccgtg accgcggcgg caatcgtcct gatttccaga gaactggttt gtgtatttct
     5401 cccgccagat tctttttcca cgtcatgaaa tgtggattcg aga
//
LOCUS       DATIPF010000317         8869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_61150, whole genome shotgun sequence.
ACCESSION   DATIPF010000317 DATIPF010000000
VERSION     DATIPF010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8869
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61150"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(316..663)
                     /locus_tag="VLL06_07915"
     CDS             complement(316..663)
                     /locus_tag="VLL06_07915"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS30929.1"
                     /translation="MAGFVLRKGQRLKTFATVKYRGDMIAGEGIVKDLSLSGSYITGN
                     VPVSVGIELSLQMFVPGEPELLVIDRATVKWIKRAEFGVDFHDLQPEVTDRITQLIAT
                     LVKRQHGTPPAKN"
     gene            979..1323
                     /locus_tag="VLL06_07920"
     CDS             979..1323
                     /locus_tag="VLL06_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30930.1"
                     /translation="MWGVILLVLPLLFSLTPAFAEQGFDPKYERDYNIYNPINRYQPD
                     NPLNPINAYDPKNPLNPINQYDPGNPANPINQYNPSNPYNPLNQYHPNNPLNPMNEFN
                     PSVPFQPLNRSR"
     gene            complement(2186..2956)
                     /locus_tag="VLL06_07925"
     CDS             complement(2186..2956)
                     /locus_tag="VLL06_07925"
                     /inference="COORDINATES: protein motif:HMM:NF022142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HSS30931.1"
                     /translation="MEKCVLRQDKRWRSRLTGLLIGVVAWILVAGAGSEVLGAPDTLT
                     QDFRLWAPVFFTAPISQSFIGYAEVNPRLGDDVSDLNQLILRTAIGYKLNDQWSIWQG
                     YAWNTVYDNADNKDDFTGEHRIYQQLTYRDKFEFLEAFPFIKIQSRTRLEERWIDHAH
                     GTALRPRTMLRMDVPLPQLPDWSFVTFDEIFVNLNGVGGGPEAGFDQNRWFIGFNRQF
                     TKQINIDFGYQMQIINTRKENMLNQLNNCIMINFFINL"
     gene            3493..3981
                     /locus_tag="VLL06_07930"
     CDS             3493..3981
                     /locus_tag="VLL06_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HSS30932.1"
                     /translation="MLTEFLVEDHRRLDGLMHTAMVHSGTVDDCAYGQFRAGLLRHIG
                     LEEKILLPAAQRQRGGEPLSLAVKLRLDHGALASLLMPTPTAEILATIQGILASHNVL
                     EDGPAGLYETCDSLAGPEGETILAALRAAPEVAVMRYNDSPAVMNAVHRALERAGYHL
                     HH"
     gene            4006..4395
                     /locus_tag="VLL06_07935"
     CDS             4006..4395
                     /locus_tag="VLL06_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSS30933.1"
                     /translation="MRAAQRLGKQLQRLRTGRGLTQEQLAVNVGLSRTFITRLELGQH
                     DPTLSTLVRLAKALRVSVTDLLGESMSASLWWQVGEQRFATREEAEDHARPLGDMFIA
                     RYQNKPAGPVRRLFPVREKDTIGARKK"
     gene            complement(4457..4771)
                     /locus_tag="VLL06_07940"
     CDS             complement(4457..4771)
                     /locus_tag="VLL06_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HSS30934.1"
                     /translation="MKLQPLHDWVLIRTGEPSGKSYGGIIIPDSAQEKPVEGEVLAVG
                     AGRFVEDKDSKEKVKKKTFVKTTLKPGAHILYEKHAAREVEGDDEELVLVREEDVLGY
                     RL"
     gene            4974..5201
                     /locus_tag="VLL06_07945"
     CDS             4974..5201
                     /locus_tag="VLL06_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30935.1"
                     /translation="MPLLILLLVMMANVPACNPAPTPGPATQQSSKSSLPLSDRGNQP
                     GKLITIILAMDRVRRATGWETPASIPVQLHT"
     gene            5216..5551
                     /locus_tag="VLL06_07950"
     CDS             5216..5551
                     /locus_tag="VLL06_07950"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS30936.1"
                     /translation="MPFMLRPFRRFPVQCAVTYNVGPFQGHGTVWNLSCTGWRLSGDL
                     PMRPGETLSLIVTLPNEQRIEVLQAVVRWSRHQEFGVETVETSKHTEARLIHYVRRLA
                     HESVEILHE"
     gene            5558..6385
                     /locus_tag="VLL06_07955"
     CDS             5558..6385
                     /locus_tag="VLL06_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30937.1"
                     /translation="MTLLKEWWQWFKRQSWLNKVIIVGLSSSLVLFLIPLAVSWLKPT
                     PVHVGVAFYIYERAFILDGDTKTYFYENRNLVSECSLRRIEQTLPKSRAKTPAPQSWV
                     LSKFLLENVSGQDITNFRMGVRSPLLHPSTTVVTTRDIEATGTLDTTAQNAPPTYVIA
                     VPVMAASAASVLILKTPIDEKVRRFLYVDRGRVTIQVPFLSADQFGTFPLKISRLNAM
                     KILNRESVLRTGDETFADEKIEFEMLASDEPDLHNEDMSYQLLPKARACPEGTAGDW"
     gene            6659..7345
                     /locus_tag="VLL06_07960"
     CDS             6659..7345
                     /locus_tag="VLL06_07960"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSS30938.1"
                     /translation="MNDVTNNVSTHVLRHFSAAICRTLVILAALVALFGPSGTGLASA
                     ESTPTESVKRTIADVVRILDNAELKQPTSAIERRRQIEHVVKDRVSYEEMAKRSLGQP
                     WAELTKTQQKEFVALFVQLLRDTFAGRIDDYTGEQVVYLSEQREGQFAEVKTQLTGDK
                     VDTLLDFRLANRQGTWLVYDVVIDGASIVGNYHAQFTSLIRDLTYQGLVEKMKEKTLV
                     AKAFEMTPAP"
     gene            complement(7474..8043)
                     /locus_tag="VLL06_07965"
     CDS             complement(7474..8043)
                     /locus_tag="VLL06_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSS30939.1"
                     /translation="MESVMLPLGSPAAPFTLRDVATGQVYSLDSFAGKSAMLVMFLSR
                     HCPYVVHVQMEIARIGQDYANSTLGLIGISSNDPMHYPEDAPERLKEMAQRLHFRFPF
                     CFDDTQEVAKAYKAACTPDFYLFDSRQRLVYRGQLDGSRPGNNKPVTGLDLRAAIDAV
                     LTGSPVDGNQHPSVGCSIKWKPGNAPSYA"
     gene            complement(8259..8471)
                     /locus_tag="VLL06_07970"
     CDS             complement(8259..8471)
                     /locus_tag="VLL06_07970"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HSS30940.1"
                     /translation="MKTKKSHALLDEQDLSPEPESMQKYSNELRERIAKKAYELFEQR
                     GRQPERHVEDWLKAEEIIRKEMNDNR"
BASE COUNT         2028 a         2435 c         2294 g         2112 t
ORIGIN      
        1 atggcgaatc gagagagaga attccggact ttaagtatga atgcactggt ggacatgcta
       61 ttcatccatg gtgtgtatga tccgaccgaa acacggttcc ggcatccctg ctagaacggc
      121 atgtcgcact atagggcaat cgcttcgttg ggatcttatc cgagcgtctg agtctcttga
      181 cagggatagg ctaggcagca tgtgacttgc tttggcgtcc ggtacacttt gccaaagccc
      241 cctgctggca tcggacgagt attgttttat cgcgtgcgtc ctgagagcaa gaaggacacc
      301 tggccgctcc ggactctaat tctttgccgg cggtgtccca tgctgtcgct tgaccagcgt
      361 agcgatgagc tgtgtgattc ggtccgtcac ttccggctgg agatcgtgga agtccactcc
      421 aaactcagcc ctcttaatcc atttcactgt agcacgatcg atcactaaca actctggttc
      481 tcccggcaca aacatctgca gggaaagttc tattcccacg gaaaccggta catttccggt
      541 aatgtagctc ccgctcagcg aaagatcctt gacaatccct tcaccagcaa tcatgtcccc
      601 acggtacttc accgtggcga atgtcttgag ccgttgacct ttccgaagta caaaccccgc
      661 catactgact ccttgtctct atcttcggat ggttgcgctc aattcgttcg gctgcttatg
      721 agccataaca acccaggcac acggcggtcc gatgcgtgat ggccctttgg ctggggcgat
      781 tgtactacct aaataagatt tatcaacagc taattccccc aaccctcctg gctctggtac
      841 ctattgacac agtctttcac aacactctgc caataggaga gaccaggctg aaggctcaac
      901 catgagagga gaactgcacg acgttccagt aggggagtat gcttggggca tgggcaatcg
      961 catatttcga ggcccgcaat gtggggtgtt atccttcttg ttctaccact gctcttctcc
     1021 cttacacctg cgtttgccga acaaggattc gatccgaagt atgagcgcga ctacaacatc
     1081 tacaatccga tcaacagata tcaacccgat aatccgctaa acccgatcaa tgcctacgat
     1141 cccaagaatc ccttgaatcc catcaatcag tacgatccag gcaatcccgc gaatccgata
     1201 aaccaataca atccgagcaa tccctacaat cccctcaacc aatatcatcc caacaacccg
     1261 ctgaacccca tgaacgaatt taacccttcg gtgccgtttc agccgttgaa ccgttcacga
     1321 taaaccgttc aacctgttta tcgttcaaga aattctcgac aaaacaacag taccgtgagt
     1381 tatttctgac caaaaactcc gaatgaccat caaaaggtat cagagacagg gctggtggtg
     1441 tgtgttcgat ccgtaaaggg attacgacca gattactacg tatgtatttg attatacacg
     1501 ataaaatcat catgatttcg caagatattt catgttatga ttttcttgct gctttcttgt
     1561 ggctagggcg acttcccctg ccgcagtgga agtcggggag gtgatgtggc catcacctcc
     1621 ccaatttttt gctctgactg cttgttgtag tgatcttagt ctgccgaaat tcgtctctta
     1681 cgctgtctca ttcctcatct gactaaccaa cacgagtcct gcccttcgag agtagaacaa
     1741 gtcttgtccg tcgtcctgct ggattctgga gcgcgagaat tcgtcaattc ctggcacacg
     1801 agcttatgga atattcttcc gggaaagatt ccgattggtc agtcctgtgc gagattcaga
     1861 tcgagcgaga gggatcgagg aggagggaag ctgaggaggc gacgtggccg ccgcctcctc
     1921 atgaagcgcc gactacttct tcttcttcgc tggcttcttt gctgctgctt tcttcgtcgc
     1981 catgagactt ccacccccct tcgatatcta aatgtgtatg ctgcacatgg tatagcagag
     2041 ttttcgaaat gtgggaagtg ccagattttg gatttcttgg gcggaggtgc gagaactgca
     2101 catagaagac tgacgcacag aggatcgcct catgcgctga ccagtctgac tcatcatgaa
     2161 tgcgctcgtt atgaagccag tgctgctaca aattgataaa gaagttgatc atgatgcagt
     2221 tgttgagctg gttgagcata ttttctttac gggtgttgat gatctgcatc tgatagccga
     2281 aatctatatt gatttgcttc gtgaactggc gattaaaacc gataaaccat cggttctggt
     2341 cgaatcctgc ttcaggaccg ccaccaaccc cattcaggtt gacgaaaatt tcatcgaacg
     2401 tcacgaacga ccagtctgga agctggggca gcgggacatc catgcgcaac atcgttcgag
     2461 gacgcagtgc ggtgccatgc gcatggtcta tccatcgttc ttccagcctg gtgcgacttt
     2521 gaattttgat gaatggaaat gcttctaaga attcaaattt gtctctgtac gtcagttgct
     2581 gatagatacg gtgttctccg gtaaaatcat ccttgttgtc ggcgttatca tagacggtgt
     2641 tccacgcgta cccctgccat atagaccact gatcgttcaa tttgtatccg atcgctgtgc
     2701 gaagaatcag ttgattgaga tctgatacgt cgtccccgag ccgtggattg acctccgcgt
     2761 atccgataaa ggactgagag atcggagcgg tgaaaaagac cggcgcccac agacggaagt
     2821 cttgcgtcaa ggtgtcggga gccccgagca cctccgaccc ggccccggcc accaggatcc
     2881 atgccactac cccaattaat agaccggtca ggcgtgaacg ccatctctta tcttgacgta
     2941 ggacacactt ctccatgttc ttctcctctt ccatagcaag atgttaagac atccgtcagt
     3001 agatccagac gattagtgga tcgattctgg tcatgtaatt gagcggcacg agccagattc
     3061 accaagtcga tcacatcaac cggataaatg gcgtacgagg aggacagcga tggtgagcat
     3121 cttacgaagc gtactcctgt tctcgttggc ctgcagagcc atgcaaactt cagttcggac
     3181 acagaatatt tccgtagcct acctgacgag cggcacctct tatagcgaat ttcgtagcag
     3241 gatgcaatca tctgttcctt gacagtcaga agggctccag acggctctgg cagctgatgg
     3301 aggcatgcca agcgatacat ggttttcgca cagtgttgga aaaatcagac aggccaaaat
     3361 agccggacag atcaaacaaa ccatcctccc taagaagatg gctggcagtt cgggcaccag
     3421 attctatatt atgcgaggct gaatccaagt tggagtcggc tgatgggaca accagaaaga
     3481 gagagggcgg gaatgctcac ggaatttctt gtggaagacc accggcggct tgatgggctg
     3541 atgcatacgg ctatggttca cagcggcaca gtcgatgact gcgcctacgg gcaatttcgt
     3601 gctgggctcc ttcgccatat tggcttggaa gaaaaaattc tcttacctgc cgcccagcga
     3661 cagcgcggcg gggaaccact gtccctggct gtcaaacttc ggcttgacca tggagccctg
     3721 gcctcgctgc tcatgccgac gccgaccgcg gaaattctgg ccacgatcca aggcattttg
     3781 gctagtcata atgtattgga ggacggaccg gccggtttgt atgaaacctg tgactcgttg
     3841 gccggcccgg aaggggagac gatcctggct gccctccgtg ccgcgccgga agtggccgtg
     3901 atgcggtata acgacagccc tgccgtcatg aatgcggtac atcgagcgtt ggagcgagcg
     3961 gggtatcatc tccaccacta gtattcgttg caaacgagag gagccatgcg cgccgctcag
     4021 cgtctcggca aacaactcca gcgactccga accggccgtg gtctgacgca ggagcagctc
     4081 gccgtgaacg tgggcctgtc gcggacattc atcacgcggc tggagctggg acagcacgac
     4141 ccgacgttga gcaccttggt gcgattggcg aaggcgctga gggtgtcggt gacggacttg
     4201 ctaggggaaa gcatgagtgc aagcctgtgg tggcaggtag gggagcagcg cttcgcgaca
     4261 cgagaggagg ctgaagacca tgcccgccct ctgggcgaca tgtttatcgc ccgatatcag
     4321 aacaagcctg caggccctgt gcgtcgtctg tttcccgttc gcgagaagga tacgatcggt
     4381 gccagaaaga agtagtttgg gtggcagcca gatttccgga ggtaaattca gcccaaagct
     4441 tgtcaaagca ggtcaattat agacggtagc ccaagacatc ctcttcacgc accagcacca
     4501 attcttcatc gtcgccctcc acctctctgg cagcatgttt ttcatagagg atatgcgcgc
     4561 ccggcttgag cgtggtcttt acaaatgttt tcttcttgac cttctccttg gaatccttgt
     4621 cctcgacaaa gcgaccagcc cccacggcaa ggacctctcc ttcgaccggt ttctcttgcg
     4681 cgctgtccgg aatgataatc ccaccatagc tcttaccgct cggctcgccg gtacgaatca
     4741 atacccaatc gtgcaacggt tgtagtttca tagaggtcgc tcctttcatt cgtacgagga
     4801 atgtaccggg ctgtctccaa tcccggcata ccgcagacca tgatttgagg cggctccctc
     4861 agcttagcac gtctaccagt gataaacgca cgacaagaag tcaccgagtc ctccttgacg
     4921 cactaccagg ccaaggacta ggctgaagat tcggtacaac tgggaggtgc cttatgccac
     4981 tcctcattct actcctcgtg atgatggcca atgtgcctgc gtgcaatcct gccccgacac
     5041 ctggaccggc gacacaacaa tcctccaagt cttccctacc attatccgat cggggcaatc
     5101 agccgggaaa gctgattacc attattctgg cgatggatcg ggtgcgacgt gcaacagggt
     5161 gggagacgcc ggcttctatt ccagtccagc tccacaccta gactgagagg ccctcatgcc
     5221 cttcatgctc cgtcccttcc gccgctttcc cgtgcaatgc gctgtcacct ataacgtcgg
     5281 accattccag ggccatggca cggtctggaa cctctcctgc accggctggc gactttccgg
     5341 cgatctgccc atgcgaccag gcgaaacgct gtctctcatc gtgacacttc cgaatgagca
     5401 acgaattgaa gttctccaag cggtcgtgcg ctggtcgcgt catcaggagt ttggggtcga
     5461 gacggttgag acgtcgaaac atacggaggc tcgtttgatt cattacgtgc ggcgattggc
     5521 gcacgagtcg gtagagattc tccacgagtg accgggtatg acgctgctga aagaatggtg
     5581 gcagtggttt aagcggcaaa gttggctcaa caaagttatt attgttggac tatcgtcctc
     5641 gttggtgctg tttctcatcc cattggctgt atcctggctc aaaccaaccc cggtccatgt
     5701 cggcgtagcc ttctatatct atgagcgtgc cttcattctg gacggggaca ccaagacata
     5761 cttctatgaa aaccgaaacc tggtttccga atgcagtctc cggcgaatcg aacagaccct
     5821 gccgaaaagc cgtgccaaga cccccgctcc gcagtcttgg gtcttaagta agtttcttct
     5881 tgagaacgtg tccggccagg acatcacgaa tttccgtatg ggtgtcaggt ctccgctcct
     5941 ccatccaagt accacggtgg tcacgacaag ggatattgag gccacaggaa cgttggacac
     6001 gactgcgcag aatgccccgc ccacctatgt gattgcagtt ccggtcatgg cagcttcggc
     6061 cgcatcagtt ctaatcttga agactccgat cgacgagaag gtgcgacggt ttttatatgt
     6121 ggatcgtggc cgggtgacga ttcaggtgcc gtttttatcc gcggatcagt tcgggacgtt
     6181 tcccctcaag atatcccgcc tcaacgccat gaagatctta aaccgggaga gtgtcctgcg
     6241 aaccggcgat gaaacgttcg cggatgaaaa gattgagttt gagatgctag cctcagacga
     6301 accggatctt cacaatgaag atatgtcgta tcagcttctc ccaaaggcgc gcgcatgtcc
     6361 ggagggaacg gcgggggatt ggtaggaatc ggtaaccgga cacatgagcc cctctccaga
     6421 attgaggcaa taaggctaca cctgaggtct ttttcgtgtt tcctgccaca gcgctccagt
     6481 gcctcctcac cgctgctctg tattagacaa ttccaacctc tacgcataga tccgcggctt
     6541 catgtgtctt ttccccggtc gcaccttgga gaaatagtct cttggccact cagcatgggt
     6601 cttgctctcc tgtactatcc gtaacgtgtg cttgacactt cgtgagagga gacgaacgat
     6661 gaatgatgtg accaacaatg tttccactca tgttctacgc catttctccg ccgctatctg
     6721 taggaccttg gtgatcttgg cagccctggt cgcgctcttt gggccgagtg gaaccggctt
     6781 ggcttcagcc gaatcaactc ccactgaatc agtgaagcgc acgatcgccg atgtggtccg
     6841 tatcctcgac aatgcagagt tgaaacagcc taccagcgcg atagaacgcc gccggcagat
     6901 cgagcatgtg gtcaaggacc gagtgagcta tgaagaaatg gccaaacgtt ctctgggcca
     6961 accctgggca gaactgacca agactcaaca gaaggaattt gtcgccctct tcgtgcagct
     7021 acttcgtgat acatttgccg gcagaattga cgactatacg ggagagcaag tggtctatct
     7081 gtctgaacag cgagaagggc agtttgcgga agtgaaaaca cagctcacgg gcgacaaggt
     7141 ggacacactc ctcgatttcc gtctggcgaa ccggcaaggc acttggctgg tctacgatgt
     7201 cgtcatcgat ggcgcgagca tcgtcggcaa ttaccacgca caattcacca gcctaatccg
     7261 cgatctcacc taccagggtc tggtggagaa gatgaaggag aaaacgctcg tagccaaagc
     7321 gtttgaaatg acgccagcgc cttaagcgcc ggcgtcatcg ttcttcccgg tttacacatt
     7381 ctcctcggac ttttcactcc aatccagcaa aattcctgtc ttgtgaaaga acacaaggga
     7441 gccaacttcg atgtctatag gaggtgcccc ctgctaggca taggatgggg cgttgcctgg
     7501 cttccatttg atactacagc cgacactggg gtgttgattc ccgtcaaccg ggctcccggt
     7561 aagcacggca tcgatcgcgg cacgaagatc gagaccagtc accggcttgt tgttgccagg
     7621 gcgacttccg tcgagttgtc cgcgatagac taaccgctgc cggctgtcaa agaggtaaaa
     7681 gtcaggggta caggctgcct tgtaggcctt cgcgacttct tgggtgtcgt cgaagcagaa
     7741 tggaaaacga aagtggagac gctgggccat ctccttgagg cgttccgggg catcctcagg
     7801 atagtgcatg ggatcgttgc tgctgatacc aatgaggcca agcgtgctgt tcgcatagtc
     7861 ctgtccaatc cgggcaatct ccatctggac atgcacaaca tagggacaat gccgactgag
     7921 gaacatgacc agcatagcgg actttccagc aaacgagtcg agcgagtaga cctgacctgt
     7981 cgccacgtcg cgtaaggtaa agggggccgc tgggcttccg agcggaagca ttacggattc
     8041 cattgccatc gaaacctcgt gccatcattc ggggaaacac aattaccgcg aagcgtcagg
     8101 tggggacacc tagacctgtg gggcgcttgg gcaagaagct gatgctgatt gggtcggcgg
     8161 ggtctgcagg tcggtgtcat cgtcgatccg actgtcccgc acaacctata gcccagtcag
     8221 cacacacaat ggatcggcca gggcggggag tcatttcatc aacgattatc attcatctct
     8281 ttgcggatga tttcctcggc tttcagccaa tcttccacat gtcgctccgg ctgtcgccca
     8341 cgctgctcaa agagttcgta cgctttcttc gcgatccgtt ccctgagctc gttcgaatac
     8401 ttctgcatgg actcaggctc aggcgataga tcctgctcat caagtagggc gtgagatttc
     8461 ttggtcttca tggacatccc tccaatgacg gctgcgtttt ttatcctaag aagaaccaat
     8521 cggcctgtca agctgtgaca agttacaggt agtgggctag cttcaaaagt acatgcggcg
     8581 gatcacaaat cgcttaatgt ttagagtgac aattgaagag gctagttcat ggggcaaatc
     8641 caaaatagcc cactacccaa gttacgcgcc ggccttgcat cgtcaatctc tgccctccta
     8701 caatgtatat ccctgcgagg cccccatgca ttcccctatt cggcactacc gccgtgtcct
     8761 tctgccctgt tctgtgagtt cccacgccgg tgcatttttc tcacaaccgc aggcctcctt
     8821 cttgagtggt tacttcctgt tcatcctcct attactgaat cctgtgccc
//
LOCUS       DATIPF010000318         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_234996, whole genome shotgun sequence.
ACCESSION   DATIPF010000318 DATIPF010000000
VERSION     DATIPF010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234996"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..655
                     /locus_tag="VLL06_07975"
     CDS             242..655
                     /locus_tag="VLL06_07975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS30941.1"
                     /translation="MLKALVDQDQFRQVFWNMATNAFEAMPEGGQLTISTSSRHVDAG
                     GRKGDVIEIAFQDTGEGIPKQNLDKIFLPFFTTKKEGSGLGLAAVHRIVDLHGGWIKV
                     ESQERQGSRFVVCMPRSGDTGVRLWHEGRTPWKRS"
     gene            640..2022
                     /locus_tag="VLL06_07980"
     CDS             640..2022
                     /locus_tag="VLL06_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSS30942.1"
                     /translation="MEKILVVDDEKGLREVLSIMLKRTGYAVTEASDGEEAIGHINKE
                     IFDLVITDLRMPKADGMAVLKAVKSSSPETVVLVVTAFATADSAVDAMKQGAYDYLTK
                     PFQVDEVQLIIRNALEKRRLSTENILLKREMASQSSFAQLVGQSESMQKVFDVVRQVA
                     DAKSNVLICGESGTGKELVARAIHYNSARSSMPFVAVNCSAVPETLLESELFGHMKGS
                     FTGAMANKAGLFEVANGGTIFLDEIGDTTPTIQVKLLRVIQEREFRRVGGNQDVKVDV
                     RIVAATNKDLEKAVADGSFREDLYYRLDVIPIRLPPLRLRTGDIPLLVNHFLGKFSQE
                     SGKPVPVLTSEAMHVLLGHEWRGNVRELENLIERVVTFSTGAPVSDEDIRGWLHRVVT
                     PQQQGVPTDLPEEGLDLEELINGIEKDLLLKALERAMWVKKKAARLLKLNTRSFRYRL
                     EKYSIKGGRD"
     gene            2159..>2438
                     /locus_tag="VLL06_07985"
     CDS             2159..>2438
                     /locus_tag="VLL06_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30943.1"
                     /translation="MSIKICADRQGIQLRCDATQLATDHSNLVYRAAALVLSRAQQSI
                     GLDIELRKRIPMGAGLGGGSSDAAATIVGLNQLLRLEWSPKQMADIGQS"
BASE COUNT          550 a          604 c          742 g          542 t
ORIGIN      
        1 ctgactgtca gcgcggtcaa gagcgtgtcg gtgctgcacg cctcctaccg ggtcgccgcc
       61 cgccagggag gccaccaggc tcgacaacat catcaccgaa tttctccaat atgcgcgtcc
      121 gccggcgctc aatcttgcgg agcgtgatct caacaaagta cttgctgaga cgctcgatct
      181 cgttcaacat gaagcacgaa cacgggtcaa gatcaaaatt gtcacgtccc tggcaaccgg
      241 agtgctcaag gctctggtcg atcaagatca gttccggcag gtcttctgga atatggcgac
      301 aaatgcattt gaagcaatgc cggagggtgg acagttaacg atctcgacga gcagccgaca
      361 tgtggatgcc ggaggccgca agggcgacgt tattgaaatt gcgtttcaag acaccgggga
      421 aggtattccg aaacagaatc tcgataagat ctttcttcca ttttttacga cgaagaagga
      481 aggctcgggg ctgggtcttg ctgccgttca tcgcatcgtc gatttgcatg gaggctggat
      541 taaggttgaa agtcaggagc gtcaaggctc aaggtttgtc gtatgtatgc cgcgttccgg
      601 ggataccggg gtgcgattat ggcatgaagg acggacgccg tggaaaagat cctagttgtt
      661 gatgacgaga aggggctgcg cgaagtcctg agcatcatgc tcaaacgcac gggctatgcc
      721 gtgaccgaag cgtcggatgg tgaagaggcc atcggtcata tcaacaaaga gattttcgat
      781 ctggtcatca ccgaccttcg catgccgaaa gccgatggca tggcggttct gaaggcggtg
      841 aagtcttcct ctcctgaaac cgtagtcctc gttgtgacgg cgtttgcgac tgcggactcg
      901 gcggtcgatg cgatgaaaca gggtgcatac gactatttga ccaagccgtt ccaggtcgat
      961 gaagtgcagt tgatcatccg caacgccttg gagaagcggc gtctctcgac ggaaaatatt
     1021 ctcttgaagc gtgaaatggc gagccagtcg tcgtttgcgc agctcgtcgg gcagagcgaa
     1081 agtatgcaga aggtcttcga cgtggtacgc caagtcgcag acgccaagag caatgtgttg
     1141 atttgtgggg aaagtggaac ggggaaagag ctggtggcac gcgcgattca ttataatagt
     1201 gccaggagtt cgatgccctt tgtggcggtg aattgcagtg ccgtgccgga gacgttgctc
     1261 gaaagcgaac tctttgggca catgaaggga tcctttaccg gcgcgatggc caataaggcg
     1321 gggttgttcg aagtcgccaa cggtggcacg atctttctgg atgaaatcgg cgatacgact
     1381 ccaaccattc aagtcaagct gctgcgcgtc attcaggaac gagagttccg gcgtgtgggc
     1441 ggcaatcaag atgtcaaggt cgatgtgcgc attgtggccg cgaccaataa ggatcttgaa
     1501 aaagcggtgg ccgacggttc ttttcgagaa gatttgtact accgcttgga cgtgattccg
     1561 attcggctcc caccgttgcg gcttcggaca ggagacatcc cgcttctcgt gaaccatttc
     1621 cttgggaagt tttcccagga gagcggcaag ccggtgcccg tgttgacatc ggaggcgatg
     1681 cacgtcttgt tgggtcatga gtggcgaggc aacgtccgcg agcttgaaaa cttgatcgag
     1741 cgcgttgtga ccttttccac cggtgctcct gtgtcggatg aggatatccg tggctggctt
     1801 catcgtgttg tgactccaca acagcaggga gtgcccaccg atttgcccga agaggggttg
     1861 gatttggagg agcttatcaa cggcatagaa aaggacctgc tcttgaaggc gctcgaacgg
     1921 gccatgtggg taaagaaaaa ggccgcacgg cttctgaaac tcaatacgcg atccttccgc
     1981 tatcggttag agaaatattc tattaaggga ggccgcgatt aatccgcagc cgaatccctc
     2041 cgagatcacc gtcttcgctc ctgccaagat caacgtgatc ctgcgtattc tcgaccgccg
     2101 ccccgacgga ttccacaatc tctggtcaat catgcagacc gtggctcttg aggacgaggt
     2161 gtccatcaag atatgtgccg accgacaggg tattcagttg aggtgtgatg cgacgcagtt
     2221 ggcgaccgac cacagtaacc ttgtctatcg agccgctgcg ttggtgttgt cacgagccca
     2281 gcaatcaatc ggtctagaca ttgaacttcg gaagcgcatt ccgatgggag ctggcctcgg
     2341 cggtgggagc agtgatgccg ccgccaccat tgtagggttg aaccagttat tacggctgga
     2401 gtggtctccg aagcagatgg cggatatcgg gcaatcgc
//
LOCUS       DATIPF010000319         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1291998, whole genome shotgun sequence.
ACCESSION   DATIPF010000319 DATIPF010000000
VERSION     DATIPF010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291998"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /gene="hisZ"
                     /locus_tag="VLL06_07990"
     CDS             complement(<1..1253)
                     /gene="hisZ"
                     /locus_tag="VLL06_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HSS30944.1"
                     /translation="MVSAPPKRRPSSGQAPSFRERSLVPVGMATILPHAAKQVRRLEE
                     QLLAHVNGWGYEEIILPTFEYLDVLAPGLEPELIEHCYQFVDRTTGRTLLLRPDATAQ
                     IARTVAMGLTGATLPLRLSYRTSVFRYEREHAGRGREIFQVGAELIGLDDVTSDSEVI
                     GLMIECLHTIGLQSFTVSLGHVGFIKGLLMRSGLSAQGQKLAEQAAARKDLPRLEEVL
                     AHERISKKAARTIREAPELYGQDEVLARGRVLAAGDPALVAPLERLAQVYHLLCAAGH
                     RDSLLLDLGEFRGFDYYDGVVFDVFAEGMGAELGGGGRYDHLIGRFGRPLPSTGFGLD
                     IDRLFRTVEFPEEGSVSGRVDYLVVAPRRATRLLMAVAAQLRRTRSRVVQQTVKGSDA
                     ALVSTAVTAGLALQAAAVVVVGAPG"
     gene            complement(1281..>2300)
                     /locus_tag="VLL06_07995"
     CDS             complement(1281..>2300)
                     /locus_tag="VLL06_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSS30945.1"
                     /translation="ELFRRAEIISVHTPLTPETKGIINAQAIATMKPGVMIVNCARGG
                     IIHEGDLFEALKSKHVAAAAFDVFEEEPVKADNPLLTLENFICTPHIGAQTAEAQENV
                     AVGIAEQIVDYFTKGIARGAVNIPSVSPELLPRLQPYLSLAEQLGKFQTQLCEGGIES
                     LTVEYSGEVATLSVASLTIAVLKGLLTPIMEAPVNYVNAPIVAKERGIQVKEVKTSDA
                     GDFTSLIRVRVQAGKKPYQVAGTLYHKKDPRVVEIDQFHVEVVPEGHMLLIHNIDRPG
                     VIGMVGKVLGDNQINIVRMQCAIEKRGGNSMLIIGSDAAFPATVLETIKSSKDILSVK
                     VAALS"
BASE COUNT          515 a          720 c          601 g          464 t
ORIGIN      
        1 attcctggcg caccgactac caccacagct gcggcctgca gcgccaaccc cgccgtcact
       61 gcggtagaca caagcgctgc atcactcccc ttcacggtct gctgcacgac acgggatcga
      121 gtccgacgca actgtgccgc aaccgccatc aacaaccgag tcgctcgacg tggcgcgacg
      181 acaagataat ccactcgccc tgagaccgat ccctcctcgg ggaattccac ggtacggaag
      241 agccggtcga tgtccaagcc aaaaccggtt gacggcagtg gccggccaaa tcgcccaatc
      301 aagtgatcgt atcgtccacc accgcctaac tccgccccca tcccttcggc aaacacgtcg
      361 aagacgacac catcataata gtcgaacccc cggaattccc cgaggtccag aagcaatgaa
      421 tcccggtggc ctgccgcaca tagtaagtga tatacctgag caagccgttc gagcggagcg
      481 accaatgccg gatcgccggc cgcgaggacc cggccgcggg ccagtacttc gtcttgaccg
      541 tacagctcag gcgcttcccg aatagtgcgc gctgccttct tggatatccg ttcgtgtgca
      601 aggacctctt cgagcctcgg gagatctttt cgcgccgccg cctgctcggc caatttctgc
      661 ccctgcgccg acagtccgga tcgcatcaga agccccttaa taaagcccac atgtccaaga
      721 gatactgtaa aggattgaag accgatcgtg tggagacact cgatcatcaa accgatgact
      781 tcgctgtcac tggttacatc gtctagccca atgagctccg ctcctacttg aaaaatttct
      841 cggccgcgtc cggcatgttc ccgctcgtag cggaagacag atgtccgata agaaagacgc
      901 aacggcaacg tcgcgcccgt gagccccatg gcgactgtcc tggcgatctg tgccgtggca
      961 tccggacgaa gaagtagcgt ccgacccgtc gtacgatcga cgaattggta gcaatgttca
     1021 atcaattccg gttcaagccc cggagccaag acatcaagat actcgaacgt gggaagaata
     1081 atctcttcgt agccccagcc gttgacgtga gcgagcagct gttcttcaag gcggcgaact
     1141 tgcttggccg catgggggag aatcgtcgcc atgcccacgg ggacgagcga acgttcgcgg
     1201 aacgacgggg cctgtcccga agaaggacgg cgcttcggag gagcggagac catggatgac
     1261 ctgatggatg gcgcgcgggg ttaggataga gccgcgactt tgacagagag aatgtccttg
     1321 ctagatttga tcgtctcaag caccgtagct gggaacgctg cgtcggatcc aatgataagc
     1381 atggagttcc cgccccgctt ctcgattgcg cattgcatcc ggacgatgtt gatctggttg
     1441 tcgccaagca ccttccccac catcccgatc acccccgggc gatcgatatt atggatcaag
     1501 agcatgtgac cctccggcac aacctcaacg tggaattgat cgatctccac gacgcgcgga
     1561 tctttcttgt gatagagcgt gccggccacc tggtacggtt ttttaccagc ctgcactcgc
     1621 actcggatca aactcgtgaa gtctcctgca tcggaggtct tcacttcctt gacctggatc
     1681 cctcgctctt tggcaacgat cggcgcgttc acataattga cgggggcttc cataatcgga
     1741 gtcagtagtc ccttcaagac cgcaatcgtc aacgacgcca ccgacagtgt agccacttcc
     1801 ccgctgtact cgactgtcag cgactcgatc ccaccttcac agagctgggt ttggaacttg
     1861 ccgagttgtt cggccaaaga gagatagggc tgaagcctcg gcagcaactc cggagaaact
     1921 gacggaatat tgacggctcc ccgagcaatt ccctttgtga agtaatcgac aatctgctca
     1981 gcgatcccga cagccacatt ttcctgcgcc tccgcggtct gcgcgccaat gtgaggggtg
     2041 caaataaaat tttctaacgt caacaatggg ttgtctgcct tcaccggctc ttcctcaaac
     2101 acgtcaaacg ccgcagctgc cacgtgctta ctcttcaagg cttcgaacaa atcgccttcg
     2161 tgaataatcc caccgcgcgc gcaattcaca atcatgacac ccggcttcat ggtggcaatg
     2221 gcctgggcat tgatgatgcc cttcgtttca ggcgtcaatg gcgtatggac ggaaataatc
     2281 tccgcccgcc gaaacagctc
//
LOCUS       DATIPF010000320         4135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_681771, whole genome shotgun sequence.
ACCESSION   DATIPF010000320 DATIPF010000000
VERSION     DATIPF010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681771"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VLL06_08000"
     CDS             <1..245
                     /locus_tag="VLL06_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30946.1"
                     /translation="FMDIAPYINDARDMDLMLLGGVTGKETDGHDWNNILTMLGWLEY
                     DHRLAHLTYNFGILLMLASFSWGGMLLLKHYRRLPP"
     gene            complement(285..1205)
                     /locus_tag="VLL06_08005"
     CDS             complement(285..1205)
                     /locus_tag="VLL06_08005"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSS30947.1"
                     /translation="MTVQPALSTATRIGWIGTGVMGSSMCGHVLTAGYRVTLHSRTKA
                     KAQPLVDLGATWAENPRTVAAQSDMIFTMVGFPQDVRTVYFSETGILAGAQSGTVLID
                     MTTTDPTLSREIAECATAKGLSAIDAPVSGGDVGARNATLSIMVGGDRRAVQVVVPLF
                     ELLGKTIVHQGGPGTGQQAKLCNQIVIAGTMIGVCESLLYGFKAGLELNRMLDSIRGG
                     AAACWTLDHLAPRILQRNFDPGFFVEHFVKDMGLALEESQRMGLVLPGLTLVHQLYHR
                     VIALGHGRSGTHALMLALEDLCRVHGNRSS"
     gene            complement(1221..1628)
                     /locus_tag="VLL06_08010"
     CDS             complement(1221..1628)
                     /locus_tag="VLL06_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30948.1"
                     /translation="MASSSSASATEPITILEIMDAPKTFHLKQVTLRGTVRDVTPLDP
                     YKLQAGTMCYGAYLFYLEDETSSINVAVYGLCGIPTVKDPDVEDGQRIELTATIQSPS
                     HGGYYLSFQGVKVAREREGVIQAVADRITPLVE"
     gene            1791..3158
                     /locus_tag="VLL06_08015"
     CDS             1791..3158
                     /locus_tag="VLL06_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HSS30949.1"
                     /translation="MIDRTRLERYVTDRRPRFEDLLGQMVEIPSISMDPAKAPDIRRM
                     ADLARQVLTDLGAEVQIVETGGYPIVSGGWMTGAHHPTVTIYNHMDVQPAQEPEWTQA
                     PFAFKNENGLYRGRGATDDKGPALSALFGARYAIEQGFPINVRFLWELEEEIGSPHFA
                     DGLKNRSAMPRPDSVVVSDTIWLAKGKPAMPYGLRGLLGARLTLRTGTKDAHSGVTGG
                     AARNPLAELMDVVHACVDAKTGKVKIPGFYQDVVEPTKAEIKSFLASGFQVKSFKQAY
                     GFQTLRTQDPAEIMRRIWAAPTFEVHGLTGGYHGPGVKTVVPGHGELKVSMRLVPNQT
                     PEKAFALLRRHVARINPNVKVEREGMLHPFKGLFEGPYVEAVKRAAKAGFGKEPAFIR
                     EGGSIGAVVTMQKAWKVPILFMGLSLPEHGYHAPNEYFDWGQASGGMKAFAHYFAELA
                     MIRKQ"
     gene            complement(3171..3869)
                     /gene="aat"
                     /locus_tag="VLL06_08020"
     CDS             complement(3171..3869)
                     /gene="aat"
                     /locus_tag="VLL06_08020"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HSS30950.1"
                     /translation="MVRLSTNPRDLRFPPVDSASPEGLLAVGGDLQPERLLEGYRHGI
                     FPWYSDDQPILWWSPDPRTVLFPDTLHISRSLKRSLRPGLFSVTLDRCFREVMQHCAE
                     PRPQYPDGGTWITVEMLEAYTRLHQLGYAHSVETWQEGQLVGGLYGVALGGAFFAESM
                     FTRVSDASKVALVSLVRQLQAWEFRLMDCQQSSPHVMALGAEAIPRREFLDHLAAALT
                     LPERRGRWQFDSLY"
     gene            complement(3926..>4135)
                     /locus_tag="VLL06_08025"
     CDS             complement(3926..>4135)
                     /locus_tag="VLL06_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30951.1"
                     /translation="KAYSLLFTPNRDNQVVFERLTEAKRWFTEVPGQTPEIWQFFLGA
                     EALKAVVDADPVFQPLLAQAEEKKG"
BASE COUNT          872 a         1153 c         1258 g          852 t
ORIGIN      
        1 gcttcatgga tatcgccccc tatattaatg atgcgcggga catggacctc atgctgcttg
       61 gcggggtgac cgggaaggaa acggacgggc atgactggaa caacattctg accatgttgg
      121 gttggctgga gtacgaccat cgcctggctc atctcaccta caacttcggc attctactga
      181 tgctggcctc attctcatgg ggcgggatgc ttctcctgaa acattaccgt cggctccccc
      241 cgtaaccctg cctccgtgac cgcgaatatt ctctaaggat cgaattacga tgacctgttc
      301 ccgtggactc tgcagaggtc ttcgagcgcg agcatcaacg cgtgggttcc gctacggcca
      361 tgccccagag ctatgaccct gtggtagagc tggtgcacca acgtgagacc gggcagtacg
      421 agtcccatac gttgtgactc ctccagtgcg aggcccatat ctttgacgaa atgctcgacg
      481 aaaaatcccg ggtcgaagtt tcgttgaagg atacgcggtg cgaggtggtc caaggtccag
      541 caggctgcgg ctccgccacg gatggaatcc aacatccggt tcagttcgag ccccgctttg
      601 aagccataga gcaagctctc gcagacaccg atcatcgtgc cggcgatcac gatctggttg
      661 cagagcttcg cttgttgccc ggtgccgggc cctccctgat gcacgatcgt tttccccaat
      721 aactcgaaga gtggcaccac gacttgcacg gctcggcggt cgcctcccac catgatcgag
      781 agggtggcgt tccgtgcacc gacgtcgcct ccggataccg gggcgtcaat cgcagagaga
      841 cccttggccg ttgcacattc ggcgatctcg cggctgagcg tcgggtccgt cgtggtcata
      901 tcgatgagta ctgtgccgga ttgtgcgcca gccagtattc cggtctcgct gaaatagacg
      961 gtgcggacgt cctggggaaa gcccaccatc gtgaagatca tgtccgattg agctgcgact
     1021 gtgcgtgggt tctcggccca cgtcgcgccg agatcgacta acggttgggc cttggctttc
     1081 gtgcggctgt gcagggtgac tcgatacccg gcggtcagca catggccaca catcgacgac
     1141 cccatcacgc cggtgccgat ccagccgatc ctggttgcgg tcgagagggc aggttgaacg
     1201 gtcatcgttc gcgtgctcgg ttattcgacg agcggggtaa ttcgatccgc caccgcttga
     1261 atgacgcctt ctcgctctct cgccaccttc accccctgaa aactcaaata gtagcccccg
     1321 tgactcgggg actggatcgt ggcagtgagt tcaattcgtt gcccgtcttc gacgtcggga
     1381 tctttgacgg tcgggattcc gcagagcccg tagacagcga cgttgatcga ggaggtctcg
     1441 tcttccaggt aaaacaaata ggcgccatag cacatcgtgc cggcctgcag cttgtaggga
     1501 tcgagcggcg tgacgtcgcg cacagtgccc cgcagtgtga cctgtttcaa atgaaacgtc
     1561 ttcggcgcgt ccatgatttc gagtatggta atgggctctg tggctgaggc ggaggacgag
     1621 ctggccacag accatccaca ccagaggaca gcgaggagac tcacgaacat tcgcgcacga
     1681 aatgtgttgc cgtatatcac ggttctattt tacagaaggt cgcagcagaa gtcccttccg
     1741 ctcattcgac gaactcgata taatccgccg acctttcttg gagaatcacc atgatcgatc
     1801 gcacacgtct tgaacgctac gtgacagacc gccgtcccag gtttgaagat ctgctgggcc
     1861 agatggtgga gattccctcg attagcatgg acccagccaa ggcccccgac atccggcgca
     1921 tggcggatct cgcacggcag gtcctcacgg acctgggagc tgaggtccag attgtggaga
     1981 ccggcggcta tccgatcgtg tccggcggct ggatgaccgg agcccatcat ccgacggtca
     2041 ccatttataa ccatatggat gtgcaaccgg cgcaggaacc tgaatggaca caggcaccct
     2101 tcgcgttcaa gaacgagaac gggctgtatc gcggtcgtgg cgcgacggat gacaaagggc
     2161 cggcgctgtc ggccctgttt ggcgcacggt acgcgatcga gcagggcttc ccgatcaacg
     2221 ttcggtttct ctgggaactg gaagaagaaa tcggcagtcc acactttgcg gatgggctaa
     2281 agaatcgatc ggctatgcct cgaccggact ccgtcgtcgt gtcggatact atctggctcg
     2341 ccaaaggaaa acctgccatg ccctatggcc tgcgcggtct gttgggtgca cgcctcacgc
     2401 ttcgaaccgg aacgaaagac gcccattctg gtgtgaccgg aggcgccgcg cggaatccgc
     2461 tagcggaact gatggacgtg gtccacgcct gcgtcgatgc caagaccggc aaggtgaaga
     2521 ttcccgggtt ctatcaggat gtggtcgaac cgaccaaagc ggagatcaag agcttccttg
     2581 catcaggttt ccaagtcaag agcttcaagc aggcctatgg atttcagaca ttgcgcacgc
     2641 aggaccccgc cgaaatcatg aggcgaattt gggccgcgcc gacgttcgag gtgcatggac
     2701 tgaccggcgg ctatcacgga ccaggagtga agacggtcgt gccgggccat ggtgaactca
     2761 aagtgagtat gcggttggtg ccgaatcaaa ctccggagaa ggcctttgcg ttgctcagac
     2821 gccatgtggc gaggatcaat ccgaatgtga aagtggagag ggaagggatg ctccacccat
     2881 tcaaaggact gtttgaggga ccctatgtcg aagcggtgaa gcgcgcggcg aaagcgggat
     2941 ttggaaaaga accggcgttc atccgagaag gcggatcgat cggcgcggtg gtcaccatgc
     3001 agaaagcttg gaaggtgccg attttgttta tgggattgag tctgcctgag catggctatc
     3061 atgcgccaaa cgaatacttc gactggggcc aagcctccgg cggcatgaag gcttttgcgc
     3121 actattttgc ggagctggct atgatacgca agcagtgaat tctgaaagca ctagtataag
     3181 gagtcgaact gccagcggcc tcgccgctca ggaagcgtaa gtgcagcagc caggtgatca
     3241 agaaactccc ggcgggggat ggcttctgcg ccgagggcca tcacgtgggg agaggattgt
     3301 tggcagtcca tgaggcgaaa ttcccaggcc tgcagttggc gcacgagtga gaccagcgcc
     3361 accttcgagg cgtcggatac acgggtgaac atcgactctg cgaagaaagc gccgcccagc
     3421 gccacgccat agagtccacc gaccaactgt ccttcttgcc aggtttcaac agaatgggca
     3481 tagccaagtt ggtgcaagcg tgtgtaggct tcgagcattt ccacagtgat ccacgtaccg
     3541 ccgtccgggt attgcggcct tggttcggcg caatgttgca tcacttcgcg aaagcaccgg
     3601 tcgagcgtga cgctgaatag gcctgggcgt aggcttcttt tcaggctgcg cgagatatgg
     3661 agtgtgtcgg gaaagaggac ggtacgggga tcgggcgacc accacaagat cggctgatcg
     3721 tcgctgtacc agggaaagat tccatggcgg tagccttcga gcaatcgctc gggttgcagg
     3781 tcgcctccga ctgcgagcaa gccttctggc gaggctgaat caacgggcgg gaaacgcaga
     3841 tcccgagggt ttgtgctgag cctgaccatg gccgagcagg gtactccaag taacgtgcaa
     3901 gactatgagc gtgtgaatag gaccattacc ctttcttttc ctctgcctgc gccagcaacg
     3961 gctggaagac aggatctgcg tctacgactg ctttcaacgc ctctgccccg aggaagaatt
     4021 gccagatttc cggagtctgt ccgggaactt cagtaaacca tcgtttggct tccgtcagtc
     4081 gttcgaagac cacctgattg tcccggtttg gagtgaacag caaactgtag gcttt
//
LOCUS       DATIPF010000321         3441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_947410, whole genome shotgun sequence.
ACCESSION   DATIPF010000321 DATIPF010000000
VERSION     DATIPF010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947410"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VLL06_08030"
     CDS             complement(<1..403)
                     /locus_tag="VLL06_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30952.1"
                     /translation="MSLIALTQITLRSTLLGSLLLLSTAGTGIGQVSSVFPGGWVEHQ
                     GTATRAPFSASQIQSFVPPTRGPFTFPSPYNTKAIRITDASDCGGTDCVIYVGYAYWR
                     NTNAHEGSNTMWIFLGLNSAKGGSGPTLFQLD"
     gene            882..2264
                     /locus_tag="VLL06_08035"
     CDS             882..2264
                     /locus_tag="VLL06_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30953.1"
                     /translation="MMNILRMRATIAIPFILILSFPLLSLMLNASAVTAPSVYSGYTG
                     VDPKPIPPPPALGPANSIVTDPTFGSRILRVTDAYTAGGMSLIPGDAGFFRTWNADST
                     ALKLMSANGKSWWVTFDPVNFKVGGGSSQPSLHPLNINHFWEWSAVDPHVLYFLNGTQ
                     LGKYNISTDTTINLGGSPTNDPVTYHVAVVGRDNWACSVAGAGTQDTYTKLFCIDPGN
                     PTQNKFIDIVKRTINGIAQTDPNWPTAASGQTIGIHSIAGSAGGSWLAVSFLRQSWGK
                     GIAVLNLTTNTWSLAKGTWAGHISLGDGKLVNGSGSTNGRDSRGAMIRDPNNMTDVTK
                     YVYLMQPPGTVGWFDAEHSSWFNASTNPNAPILFSRYNITSPPAPVPWYGEIIMAAGD
                     GSNTVWRFAHNHNGGGGYFGEAFAQISNDGRWALFSSYWDGTLGPSKGDFGIGTRIDT
                     FIVELVKPGG"
     gene            2527..3432
                     /locus_tag="VLL06_08040"
     CDS             2527..3432
                     /locus_tag="VLL06_08040"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSS30954.1"
                     /translation="MPMFGIQRCTGTLPKRVLGKTGIQLPILGFGTTAAGQRLTMREA
                     VDLYETAYCQGITYFDTAPEFAGYGKAQIQLSHVLKHVRRHVFLVTKCFEPEGERALR
                     LLDRSLRELGTDYADLVFVHSVGDDRMDPQTVFSKSGSYAALMKAKALGLTRFVGLSG
                     HSRPSRVLSAIRHVDVGVLLNAVNFADRHTYNFEEHVWPAAAHDNIGLIAMKVFGGAK
                     HPPPATVNHSQMPPEYHDLAFRYALSQPQISCAVIGMATHDELKQNLRRARTFSPLSV
                     EEARRLHQSGAILARQWSDHFGPVI"
BASE COUNT          828 a          886 c          925 g          802 t
ORIGIN      
        1 tgtccaattg gaacagcgtc ggcccggacc ctccctttgc gctgttcagt ccgaggaaaa
       61 tccacatggt gttgctgcct tcgtgcgcgt tggtattgcg ccaatatgca tagccgacgt
      121 agatgacgca atctgtgccg ccacaatctg acgcatcagt aatgcggatg gctttcgtat
      181 tatatggaga ggggaatgtg aacggaccac gcgtgggagg gacgaacgat tgaatttgcg
      241 aagcgctgaa gggtgcgcgg gtcgccgtac cttggtgttc cacccaacca ccggggaata
      301 cactggacac ttgtccgatt cccgtgcctg ccgtcgaaag caggagtaac gaacctagca
      361 gcgtgcttcg aagcgttatc tgcgtcagtg ctataagcga cattatcttt ctccaggtcg
      421 ttgatcggtc gattttcaaa cgggaactgt aaaacgtgcg tgcaataaca gccacaagag
      481 ggcatgtcca acatgatcct gatgcctcac ctcatcgtgt gacagctgac gccactcgtc
      541 attcgagtcg acgactgtgg tgactacgca aaatatgtac catcgccgga cggtgaacat
      601 cggaaccaaa tacgctgaac ttaagcggtg aagaggaaga atacagagtg gtggtctccg
      661 gtaggaccgt aggagaaggg tcggacttct gagtcaaacg ttgtctttct gagaagatat
      721 gctgttagta actgcctaca gcgacaacag ttaaggtggg gttaagaaat ggtaaagctc
      781 aggcaccgac gtcggacctt gtgtgatcca cacggaattg taggaatctc ggccagcggc
      841 tgtccgtatg aatttgcaga ggttaccgaa cgctgaagga gatgatgaac attctgagaa
      901 tgcgcgccac aatcgcgata cccttcattc tgatcctgag tttcccacta ctaagcctga
      961 tgctcaacgc ttcagccgtc actgctccct ctgtgtattc cggttatacg ggagtggatc
     1021 caaaacctat ccctcctccg cctgccttag ggccagcgaa ctccatcgtg actgatccca
     1081 cttttggctc ccgcatcctg cgggtaactg acgcctatac agcaggaggg atgtccttaa
     1141 ttcctgggga tgccgggttc ttcagaacct ggaatgctga ttcgactgca ctgaaactca
     1201 tgagtgcgaa tggcaagtcc tggtgggtca cgtttgatcc cgtcaacttc aaggttggcg
     1261 gcggatcgtc tcaaccctca ttacatccgt tgaacattaa ccatttttgg gaatggtccg
     1321 cggtggatcc tcatgtcctc tattttctca acggcactca gctgggcaaa tacaacatct
     1381 ccacggatac cacgataaat ctgggcggct cacccactaa cgatcccgtc acctatcatg
     1441 tcgccgtggt tggtcgagat aactgggcct gttcggtggc gggagctggt acgcaggata
     1501 catatacgaa actattctgc atcgaccctg gcaacccgac ccaaaataaa ttcatcgaca
     1561 ttgtaaaacg aacgatcaac ggcatcgctc agaccgatcc caactggccg accgcagcct
     1621 ccggccagac catcggcatt cattcgatcg ccggcagtgc cggtgggtct tggctcgccg
     1681 tgagcttttt acggcagagt tggggaaagg ggattgcggt gctgaacctc accacgaata
     1741 cctggtcctt ggcaaagggc acttgggcgg gacacatttc attgggtgat ggcaaattgg
     1801 tgaatggatc tggatcaacg aatgggaggg attcgcgcgg cgcgatgatt cgagatccga
     1861 ataacatgac ggatgtcacc aagtatgtct atctgatgca gccaccggga actgttggat
     1921 ggttcgatgc cgagcatagt tcttggttca atgcttcaac aaatcccaac gcaccaattt
     1981 tgttttctcg ctacaacatt acctcgccgc cagctcccgt gccatggtat ggagagatca
     2041 ttatggcggc cggcgatgga tccaatactg tctggcgatt tgcccacaat cacaacggcg
     2101 gaggcggtta ttttggggag gcgtttgcgc aaatctccaa tgacggccgc tgggctctct
     2161 tttccagtta ttgggatggc acactcgggc cttcgaaggg agattttgga atcgggactc
     2221 gcatcgacac gttcattgta gagcttgtaa agcctggtgg ctgagaggcc atgccgtgaa
     2281 agcacaaccg atccttgtgc acaacgtgat ggcctcagcg gaatcagaag ggaatatggt
     2341 gcaagcgcag tagaccttgc gaaatccgaa gcaggctcga caccaacagg tgtcgagcct
     2401 tttttctggc cagacctttc aatgagggac cgtctggaga gtaaaaagaa catataagat
     2461 aagcagcgag cttgttcatt cactcgggga attccgagta tcgcaatacg cacgaagact
     2521 tatgctatgc cgatgttcgg tattcaaaga tgcacgggga ccctgcccaa acgtgtactc
     2581 ggcaaaaccg ggattcaact gcccattctc ggattcggca ccacagctgc cggacaacgt
     2641 ctaacaatga gagaggctgt cgatctctat gaaacagcat actgtcaagg aattacatac
     2701 ttcgacactg cccctgagtt cgccggctat gggaaggccc agatccagct gagtcatgtg
     2761 ctcaagcatg tccgtcgtca cgtatttttg gtgaccaagt gtttcgagcc tgagggagaa
     2821 cgagcgttac gattgctgga tcgcagcctg cgggagcttg gaacagacta tgccgatttg
     2881 gttttcgtgc atagtgtcgg cgatgacaga atggatccac aaacggtctt tagcaagtca
     2941 ggcagctatg ccgcgctcat gaaagctaag gccttaggcc tgacgcggtt cgtaggatta
     3001 tccggacata gtcgaccatc gcgagttctt tccgctatac gccatgttga tgtgggtgtg
     3061 ttattgaatg ccgtcaattt tgcagatcgc catacctaca atttcgaaga gcacgtatgg
     3121 ccggctgccg ctcatgacaa tatcggatta atcgccatga aggtcttcgg cggcgccaag
     3181 catcctcccc cagcgactgt aaatcattcc caaatgccgc ctgaatatca tgacctcgct
     3241 ttccgatatg ccttaagcca gccacagata agctgcgctg tgattggcat ggcgacccat
     3301 gatgaactga aacagaacct gcgccgtgcc agaacgttct cacccttgtc agtcgaggag
     3361 gcaagacggc tacatcaaag tggcgcaatc ctcgccaggc aatggtcaga tcattttggg
     3421 ccagtgatat agaggatagg t
//
LOCUS       DATIPF010000322         4736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1292543, whole genome shotgun sequence.
ACCESSION   DATIPF010000322 DATIPF010000000
VERSION     DATIPF010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292543"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /gene="gmk"
                     /locus_tag="VLL06_08045"
     CDS             <1..734
                     /gene="gmk"
                     /locus_tag="VLL06_08045"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HSS30955.1"
                     /translation="RTGPKRRMNSVTATSSTAPASGQPRSSTDRRGILFIISAPSGTG
                     KTTLCKQLTANLPGLWHSISYTTRKPRPGEEHGRDYYFIEEPAFRDMIERSEFVEWAH
                     VYGHLYGTPRKALTERIDQGIDVLLEIDVQGAMQVRKRFEDSVSIFILPPSMTVLRSR
                     LQTRASDTPEEIQRRLHKVKEEVWSYREYAYIVQNEDITRSLRDLESIFWSERLKTKR
                     LNMAWLETNFILDDEQKTGDRKDLP"
     gene            755..1111
                     /locus_tag="VLL06_08050"
     CDS             755..1111
                     /locus_tag="VLL06_08050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HSS30956.1"
                     /translation="MIDMLRLLPQYSPDEFDSRHRLTIVAAQRAKHILQGSRHAPSRF
                     NKETTIALDEVLRGQVKYLVGQEARDASKEAKRGKEGEMERIAVMTGDDAKEIKKELS
                     VYVDDSPAPAAPENEE"
     gene            1115..2377
                     /gene="coaBC"
                     /locus_tag="VLL06_08055"
     CDS             1115..2377
                     /gene="coaBC"
                     /locus_tag="VLL06_08055"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HSS30957.1"
                     /translation="MELSGSIGPILIGKRIVLGVTGSIAVYKAVALLRTLLREGATVY
                     VVMTQSATKFVTPLTFEVLSGHPVSTDLFEAHQEMKHLSLPEQADAIVIAPATANCLA
                     KSALGLGDDLLSTMLLTAQCPLIMAPAMDGGMWTHPSVKEHVGTLRARGAIVVNPEEG
                     SLASGQIGQGRLAEEGTILAAIQEALAPRRDWQGHRLLVSAGPTQEPIDPVRFISNRS
                     SGKMGYAIAEAAQARGAQVVLVTGPTAIPTPKGVEVVPVATAEEMMKALSTRLAWSTA
                     VIMAAAVADFRPKHLVAQKMRKQGRTDQTLDLEQTTDILASLSAKRTTQLMVGFAAET
                     HDLIAHAKGKLTAKGLDLIVANDVTTIGAGFGSDQNAATLIDRQGGITELPLMPKRAL
                     ADAILNRAIELFLTRQSSHAPKPMVRGR"
     gene            2384..3223
                     /locus_tag="VLL06_08060"
     CDS             2384..3223
                     /locus_tag="VLL06_08060"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS30958.1"
                     /translation="MAQNQIATENAMEIDRLASQLAKDPHSKVFMPLAEEYGKVGMWK
                     EAATVLEDGLKLYPGFITAMVALGRAYDHLGQAKQAKAILEESVKLSPENLRAHRTLI
                     KIYTSEGLGESAWKSCAVILAVNPRDEEALSVQAALGGPLKQSLQPQQPKLAPPVTPQ
                     VSTPTATVPSAPAELTASVTETSTTPDLPVSTPPEPEVADTDPRMTATEMLVTALRSR
                     DQTKQSLAEPEMELPAPDRVETPPPSPHAEIVAQLESWLRTIESRRHDRDSTDDESHP
                     KPS"
     gene            3330..3881
                     /gene="aroQ"
                     /locus_tag="VLL06_08065"
     CDS             3330..3881
                     /gene="aroQ"
                     /locus_tag="VLL06_08065"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HSS30959.1"
                     /translation="MLRIQVLHGPNLNLLGTREQSIYGTQSLDALDSAITKLAKELAI
                     KVDLRQSNSEGELVTWIQKARSKYDGIIINPAAYTHTSIAIRDAIAAVGLPTVEVHLS
                     NIHQREEFRHRSHIAGVAIGQICGLGPTGYLLALRGLHDHLTTSLREKKTAAPATSRR
                     TARGTRSLRSRSSDRVQSEGSIE"
     gene            3878..4438
                     /gene="efp"
                     /locus_tag="VLL06_08070"
     CDS             3878..4438
                     /gene="efp"
                     /locus_tag="VLL06_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HSS30960.1"
                     /translation="MISTTDFRGGVRLMVDNQPFYIVEFQHVKPGKGGAFVRTKLKSY
                     LSGNVLDRTFRSGERFEEPKLDERTMQFLYATDEAYTFMDTETFEQLTFEKSKLGDNA
                     DLLKEDMTVKILVYEHRPIGVELPNFIELKVSDTEPGFRGDTATGGTKLATLETGATI
                     KVPLYLETGTVIKIDTRTREYVERVR"
     gene            4435..>4736
                     /locus_tag="VLL06_08075"
     CDS             4435..>4736
                     /locus_tag="VLL06_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30961.1"
                     /translation="MKKQKPSKSKKGGTPIILPQAFAAPQRGQGAGSLSGQHTRHIQE
                     LIDLLKKNNLTELELEREGLRIRVRNEVDVNATATTVADRGTSGSTAVTPAPTANGA"
BASE COUNT         1176 a         1397 c         1291 g          872 t
ORIGIN      
        1 cgcgaacagg tccaaaacgt cgaatgaact cagtcactgc aacaagctcc acggcgccgg
       61 cctcaggcca accacgatcc tcaacggatc gacggggaat cctgtttatt atctccgccc
      121 cctccgggac gggcaaaacg acgctctgca agcaactcac ggcgaacctt ccgggactct
      181 ggcattcaat ttcctacacc acgagaaagc cgcggccggg cgaagaacat gggcgcgact
      241 attacttcat cgaagaaccg gcattccgag acatgatcga gcgaagtgaa tttgtcgagt
      301 gggctcatgt ctacggacat ctctacggga cgccgcggaa agcactcacc gaacggatcg
      361 atcaggggat tgatgtcctt ctggagatcg atgtgcaagg ggcgatgcag gtcagaaaac
      421 gattcgagga ttccgtgtcg atctttatcc ttcctccctc gatgaccgtg ctccggtccc
      481 gactccaaac cagagcctct gatacaccgg aggaaattca acggcgactg cataaagtga
      541 aagaagaggt ttggagctac cgggaatacg cctatatcgt tcaaaacgaa gacatcacca
      601 gatcgcttcg cgatctggaa agcatcttct ggtcagaacg cttgaagaca aaacgattga
      661 acatggcctg gcttgagaca aatttcattc ttgacgatga acagaaaacc ggagatcgca
      721 aagatcttcc ctaacctaaa ggagtactgg aaccatgatc gacatgttga gactcttacc
      781 acaatattca ccagacgagt ttgactcacg ccaccggctg acgatcgtcg cagcccagcg
      841 ggccaagcac attttgcagg gctcacgtca tgctccctca cgcttcaaca aagaaacgac
      901 catcgccctc gatgaagttc tccgaggaca agtcaaatat cttgtcgggc aagaagcgcg
      961 tgatgcctca aaggaagcga agcgtggcaa ggaaggcgag atggagcgta tcgccgtgat
     1021 gactggcgac gatgccaagg agatcaagaa agaactgagc gtctacgtcg acgattcccc
     1081 agcgcctgca gcgcctgaaa acgaggagta attggtggaa ctttccggct ctatagggcc
     1141 gatattgatc gggaagcgga ttgtactggg cgtgacgggc agcattgcgg tctataaggc
     1201 cgttgcgttg ctccgaaccc tgctccgtga aggcgcaacc gtctatgtgg tgatgacgca
     1261 gtcggccacg aagttcgtta ccccgctgac attcgaagtg ctctccggcc atcccgtctc
     1321 gacagatctg ttcgaagcgc atcaggagat gaaacacttg tcgttgcccg aacaggcaga
     1381 tgcgattgtc attgcaccgg ccacggccaa ttgtctcgca aagtctgcat tggggctggg
     1441 agacgatttg ctctcgacca tgctcctgac ggcacagtgc ccgctcatta tggcccctgc
     1501 catggacgga gggatgtgga cccatccatc tgtgaaagaa catgtcggga cattgcgcgc
     1561 gaggggagcc atcgtggtga atccggagga aggatcgctt gcttccggac aaatcggcca
     1621 aggccgcttg gctgaggaag gcacaatcct ggcagcgatt caggaggctc tggctccacg
     1681 gcgcgattgg caagggcacc gtctgctggt ttcggcgggc ccgacacagg aaccaatcga
     1741 tccggtgcgc ttcatttcaa accgctcctc cggcaaaatg ggctatgcca tcgcagaagc
     1801 agcccaggcg aggggagccc aggtggtcct tgtcaccggg ccgacggcta taccaacacc
     1861 gaagggcgtc gaggttgtac cggtcgcgac ggctgaagaa atgatgaagg ccctgtccac
     1921 gcgtctcgca tggtccaccg cggtgatcat ggctgccgcc gtggcggatt ttcgccccaa
     1981 gcaccttgtc gcgcaaaaaa tgcgcaaaca gggacgaacc gaccagaccc tcgatctcga
     2041 acaaaccact gacatcctgg cctctctctc tgccaaacgg accacacaac tcatggttgg
     2101 cttcgctgcc gagacccacg atctgattgc ccatgcaaaa ggcaagttga cagcaaaagg
     2161 gctcgacctc atcgtcgcca acgacgtcac gacaataggg gcgggattcg gcagcgatca
     2221 gaacgccgcc acgttgatcg accggcaggg gggcataaca gaactgccgc tcatgccaaa
     2281 acgagcactt gccgatgcga tcctgaaccg ggcaatagaa ctatttctca ccagacaatc
     2341 cagtcatgcc cccaagccca tggtgagagg acgctagcag gctatggctc agaatcaaat
     2401 tgccactgag aacgccatgg aaatcgatcg gctggcgagt cagttagcca aagatccaca
     2461 ttccaaagtg ttcatgccat tggctgaaga gtatggaaaa gtcggcatgt ggaaggaagc
     2521 cgcaaccgtg ctcgaagacg gcctgaaatt gtatcccggc ttcattaccg cgatggtcgc
     2581 acttggacgt gcgtacgacc acctgggtca ggccaagcag gcgaaggcga ttctcgaaga
     2641 atccgtcaag ctgagccctg aaaacctccg cgcgcatcga acgttgatca agatctacac
     2701 aagcgagggg ctgggtgagt cggcctggaa atcctgcgcc gtcatcttgg cggtcaaccc
     2761 ccgggatgaa gaagccctct ccgttcaagc tgccttaggt ggacccctca aacaatcgct
     2821 gcaacctcaa cagccaaagc tggcgccgcc ggtcacgccc caggtttcca ccccgacggc
     2881 cactgtgccg tctgctcctg ctgaactcac ggcttccgtc acggaaacga gtactacgcc
     2941 ggacttgccg gtctcaacgc cacctgaacc agaggttgct gacaccgacc cgcgtatgac
     3001 agcaacggaa atgctggtga cagccctccg ctcgcgcgat cagacgaagc aatcactcgc
     3061 ggagcccgag atggaactcc cggcacctga cagggtcgag acacctcctc cgtcgcctca
     3121 tgctgaaatc gtcgcccagc tggaaagctg gcttcgcacc atcgaatcgc gtcgtcacga
     3181 tcgtgacagc acagacgacg agtcgcaccc caaaccttcc taacagtttt ggtatgtttg
     3241 accggtctga ggcggactgc tacaatgccg cccgcaggac tccgtcccga tgaatcgtct
     3301 acgcacttcc agtctgtgaa tacaaagaca tgctacgaat acaagtctta cacggtccga
     3361 atctcaatct cctcgggacc agagaacagt caatttacgg cacccagtcc ctcgatgcct
     3421 tggattcggc cattacgaag ctggcaaagg aattagccat aaaagtggac ttgcgccagt
     3481 caaatagcga aggtgaactg gtcacctgga tccaaaaggc ccggtcgaaa tacgatggga
     3541 tcatcattaa tccggccgcc tatacccaca ccagcatcgc catccgcgac gccattgcgg
     3601 cagttggcct gccgacggtt gaggtgcatc tgtccaatat ccatcagcgg gaagaatttc
     3661 gccatcggtc ccacatcgcg ggagtcgcca tcggacagat ctgcggcttg ggtccgaccg
     3721 gctatctgct cgcactccgt gggctgcacg accatctgac cacatcgctg cgcgagaaga
     3781 aaacggctgc accggcgaca tcacggcgga cggcaagagg aaccagatca ttgaggagcc
     3841 ggtcgagtga ccgcgtacag tctgaaggga gtatcgagtg atttccacaa ccgattttcg
     3901 cggcggcgtg cggcttatgg tcgacaacca gccgttttat atcgtcgagt ttcagcatgt
     3961 gaagccaggc aagggcgggg ccttcgtgcg caccaagctc aaaagctatc tgtcaggcaa
     4021 tgtcctggat cgaactttcc gatccggaga acggttcgaa gaacctaaac tcgatgaacg
     4081 gaccatgcaa ttcctctatg ccaccgatga agcgtatacg ttcatggaca cagagacctt
     4141 cgagcaactc accttcgaaa agagcaagct tggcgataac gccgacctcc tgaaagaaga
     4201 catgacggtg aagattctgg tgtacgaaca tcgccctatc ggagtcgaat tgcccaactt
     4261 tatcgaactt aaagtcagcg ataccgagcc ggggttccga ggcgacacag ccacgggcgg
     4321 caccaagctc gcgacccttg aaaccggcgc cactatcaaa gtccctctct atttggaaac
     4381 cgggacagtg attaagatcg atacccgtac ccgagagtat gtggagcgtg ttcggtgaag
     4441 aagcagaagc cgagcaaatc gaagaagggt ggaaccccga tcatccttcc tcaggccttt
     4501 gccgcacccc agcgcggaca gggcgcaggc tcactctcag gacaacacac ccgccacatc
     4561 caagaactga ttgacctcct caagaagaat aacctaaccg aactggagct ggagcgcgag
     4621 ggcctcagaa tccgcgtacg caacgaagtt gatgtcaacg cgaccgcaac gaccgtcgcc
     4681 gaccggggga cgtccggttc gactgcggtc actcccgcgc ccaccgcgaa cggcgc
//
LOCUS       DATIPF010000323         4708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1389823, whole genome shotgun sequence.
ACCESSION   DATIPF010000323 DATIPF010000000
VERSION     DATIPF010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389823"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1287)
                     /locus_tag="VLL06_08080"
     CDS             complement(145..1287)
                     /locus_tag="VLL06_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HSS30962.1"
                     /translation="MTLLLDRSAHQALLELKRVLIIDDEEPIRRLLGYMLQTHGYETV
                     LACDAREARARLEEQPFALMMCDVNMPGESGMDLVRNILSTHPHTAVIMVTGLDSSVL
                     ANAALDMGAFGYIVKPFESNEVMIDVANALRRRRLELENRLHRENLEDVVRTRTMALQ
                     QALEWLEVREKELRLSREETIQRLAIAAEYRDSATAQHIQRMSHYCELLARKYGLSSE
                     RCDLIRTASPMHDIGKIGTPDHVLLKPGKFTPEEFTVISQHAEIGYRILSGSDAEILK
                     VAASIAWTHHERWDGNGYPRKLKGEAIPVEGRIAAIADAFDALTTQRVYKPAFTLDHA
                     VDLMTKHRGEHFDPELLDVFFASMNDITRIHDQYADQSPQKLIPKP"
     gene            1763..2506
                     /gene="rph"
                     /locus_tag="VLL06_08085"
     CDS             1763..2506
                     /gene="rph"
                     /locus_tag="VLL06_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HSS30963.1"
                     /translation="MSSAAGLLRVDGRRRDQLRPVKVTRDFIKHAEGSVLIEMGDTKV
                     ICTASIEEKVPPFLKGKGQGWVTAEYSMLPRSTHERSPREAVKGKQGGRTLEIQRLVG
                     RALRAVTDMSQMGERSIWLDCDVIQADGGTRTASITGAFIALADACTVLKKRGLIKKR
                     PLTDYLAAISVGKVGGEILVDLAYTEDSMADVDMNVVMTGQGRYVEVQGTAERTPFAK
                     QEMDEFMAMGWSGIQTLTALQRDLIGDLE"
     gene            2516..3148
                     /locus_tag="VLL06_08090"
     CDS             2516..3148
                     /locus_tag="VLL06_08090"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HSS30964.1"
                     /translation="MIRELVLATRNRHKAAELLMLLGDPRITIRTLDEFPGAPDVVED
                     GDTCEANAVKKACAIAEYTGLPAVADDTGLEVDALGGRPGLYAARYAGEHATYEDNCR
                     KLLLELAGVSPAQRTARFLTVAAIALPSGRVRVAQGVLDGMIAEEANGTLGFGYDPVF
                     FVPELGKTLAQLSADQKNTISHRAKAFTQAKDLLHEMEFEMLDSGRSAAR"
     gene            3126..3202
                     /locus_tag="VLL06_08095"
     tRNA            3126..3202
                     /locus_tag="VLL06_08095"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3160..3162,aa:Pro,seq:tgg)
     gene            3567..3902
                     /locus_tag="VLL06_08100"
     CDS             3567..3902
                     /locus_tag="VLL06_08100"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS30965.1"
                     /translation="MDVKMEDRRATPRFRVQFRTTVSGSSQPEGAGIILDLSRGGCRL
                     ESPLSILPGFSLELRIHVSGLEWPLMIDEADVQWVSERSSGLAFVRIRQTEQQRLDEV
                     LKNLSTHHE"
     gene            4047..4283
                     /locus_tag="VLL06_08105"
     CDS             4047..4283
                     /locus_tag="VLL06_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30966.1"
                     /translation="MSKAVIYKGYTIQPALRHLVDTGQWELNVFISWQADEEEESRHF
                     YSTGRYATEDEATAQCIAYGQQIVDGKIPGSSVG"
BASE COUNT         1067 a         1152 c         1401 g         1088 t
ORIGIN      
        1 gacgctggca gatgcgaagc tgtaggctcg aaaaaatcgg aggcgtattc gctggaattc
       61 gttgaggatg tgttcgagct gagaatgagg aaaatgcctg cggagtattg gtcgaaggag
      121 aagggcctgc cgggaatgga cttctcaggg tttgggaatc agtttttgcg gggattgatc
      181 ggcatactgg tcgtggatgc gcgtgatatc gttcatcgag gcaaagaaga cgtcgaggag
      241 ctcgggatca aagtgttcgc cacgatgttt ggtcataagg tcgacggcgt gatcgagggt
      301 gaacgccggc ttgtagaccc gttgtgtggt gagggcatcg aaggcatcgg cgattgcggc
      361 gatccgtcct tctacgggga tcgcttcacc tttcaatttg cgcggataac cgttgccatc
      421 ccaacgttca tgatgagtcc aggcgattga tgccgccact ttgaggatct ccgcgtccga
      481 tccgctgagg atacgatagc cgatttccgc atgttgagag atgactgtga attcttccgg
      541 tgtgaatttg ccgggcttga gcagcacgtg atccggcgta ccgatttttc cgatgtcgtg
      601 catcggactg gccgtgcgga tcaaatcaca ccgctcggag gatagtccat actttctggc
      661 gagtagttca cagtaatgac tcatgcgctg aatgtgctgt gcggtggcac tgtcacggta
      721 ttcggcggcg atggcgagtc gttgaatcgt ttcctctcgc gagagacgca gttctttttc
      781 gcgtacctcg agccactcga gcgcttgctg gagtgccatc gttctcgttc tgacgacgtc
      841 ttccaggttc tcccggtgca ggcgattctc cagctcaagg cggcggcggc ggagtgcatt
      901 agcgacgtcg atcatgactt cattcgactc gaacggcttg acgatgtatc caaaggctcc
      961 catgtccagt gccgcgttcg ccagcactga gctgtcgaga ccggttacca tgatgaccgc
     1021 ggtgtgggga tgtgtactca agatattgcg gacgagatcc atccctgact caccgggcat
     1081 gttcacatcg cacatcatca acgcaaaggg ttgttcctca agccgggctc gtgcctctcg
     1141 tgcatcgcag gcgaggaccg tctcataccc atgggtttgc agcatgtatc ccagcagtct
     1201 gcgaatcggt tcttcgtcat caatgatcag cacgcgcttg agttcgagga gggcttggtg
     1261 ggcagatcgg tcaagaagca acgtcatgat tcgtgaggct cgtgtctgtc taaatatgtg
     1321 aagaagggag ttgtctagag tcggtggatg ctcgttgtgt cacgatgttt attgacctta
     1381 agagaactgt acggtcatct gcaccttttg tgccacaata ttctaaaaat ctcacacctt
     1441 gtgggcctct tgctcaatca tatcttcttt gccgagaagc agtgggatgc agcgctgtga
     1501 ttggattttc atatgtgcag gttgcacgac tgcgcacaac cgaagcagga catgcgtctg
     1561 aagtatgggc tcaccctgta cagatttagc cgttcgcgct gcgtactttt gtacaaatcg
     1621 atcgacagaa atgcttcatt cagagcacac acgattttat tatcattcgg ctccacgcgt
     1681 cgatttgtgg cgccaggccg gtttcattgc gcttttgaag ggccttcact ataatgcgcg
     1741 gcatcattaa taggagtcat ctatgagcag tgcagcggga ctattgcgtg tcgatgggcg
     1801 acgacgggat cagcttcgtc cggtgaaggt gacgcgggat ttcatcaagc acgccgaggg
     1861 gtctgtcctg atcgaaatgg gcgacaccaa agtgatctgc acggcttcga ttgaggaaaa
     1921 ggtgcctccg tttctcaagg ggaaggggca aggatgggtg acggcagaat actcgatgct
     1981 cccgcgttcg acgcacgagc gatccccgcg tgaagcggtc aaagggaaac agggtggcag
     2041 aacgttggaa attcaacgcc ttgtagggcg agccctccgt gcagtgacgg acatgagcca
     2101 gatgggagag cggtcgatct ggctcgattg cgacgtcatc caggccgacg gagggaccag
     2161 aaccgcatct attacaggag cattcatcgc gctggccgat gcatgtaccg tcctcaagaa
     2221 gcgggggctg attaagaagc gtcctttaac ggattatctt gcggccatta gcgtagggaa
     2281 ggtgggtgga gagattcttg tcgacttggc ctatacagaa gactcaatgg ccgacgttga
     2341 catgaatgtg gtcatgaccg gacaaggacg ctacgttgag gttcagggta cagcggaacg
     2401 gacccctttt gccaaacaag aaatggatga attcatggcg atgggttgga gtgggattca
     2461 gaccctcacc gcgttgcaga gggatctgat cggggatcta gaatagagaa cgatcatgat
     2521 tcgtgaattg gtgctcgcaa cccgcaatcg gcataaagca gcagaactcc tcatgctctt
     2581 aggcgaccca cgcatcacaa ttcgtacact cgatgaattc ccgggtgctc cggacgtggt
     2641 cgaagacgga gatacctgtg aggccaacgc tgtaaagaag gcctgtgcca ttgccgagta
     2701 cacgggcctg ccggctgtcg ccgatgatac agggctggaa gtggatgcgc ttggcggccg
     2761 acccggtctc tatgcggcgc ggtatgccgg cgaacatgcg acctacgaag acaactgccg
     2821 gaagttgctg ctggagttag caggcgtatc tcctgcacaa cgaaccgctc gctttttgac
     2881 cgtcgcagca atcgcactgc cttcaggcag agtaagggtg gcacagggag tcttggatgg
     2941 aatgattgca gaggaggcga atgggacgtt aggatttggg tatgatcctg tattctttgt
     3001 tccagagtta ggcaagactt tggctcagct ctcggcggat cagaagaaca cgatcagcca
     3061 tcgtgccaaa gcattcactc aggcgaagga tctcttacac gagatggaat tcgagatgct
     3121 cgattcgggg cgtagcgcag ctcggtagcg cgcctgcttt gggagcagga tgtcggaggt
     3181 tcaaatcctc tcgccccgac caaagcgtag gaacgatacg attgagagtc gagagagttt
     3241 gaagccagtg agcgccgacc gctagaagga aaaggtcgcc atatttctgg cgaccgacag
     3301 cgagctggcc tggccgaagg ccaaatcctc tcgccccgac caataccatc aattgcttgt
     3361 acattttcga caccaccaca agacgacacc gcggtgattc atagtcgtgg gattccacgt
     3421 gaaacatttt cccccttaaa tgatagccgc gacggagtaa aagaccgtcc gagaattgtt
     3481 actcaagttg attgaggaca agattcacga gttggagaag aaaggccttc gcgggtcgtg
     3541 atgggaaact ggaggactac ttggtcatgg acgtaaagat ggaggatcga cgggctacac
     3601 cccgtttccg cgtccagttt cgcaccacgg tgtcaggttc atcgcagcca gaaggggcgg
     3661 gcatcatact tgatctctca aggggcggat gccggctgga aagcccgctt tccattttgc
     3721 cgggcttctc attggagttg cgcatccatg tatctggttt ggagtggccg ctgatgattg
     3781 atgaggctga cgtccaatgg gtgagtgagc ggagctccgg tctggctttt gttcgaatca
     3841 gacagacgga gcagcagcga ctcgatgagg tgctcaagaa tctcagcacg caccacgaat
     3901 aatccaatct gcaggcagtc tcatgcctga aaatggccgt cgccatcgaa gagaaatcag
     3961 tagccgatct tggtatgggc actgatcgag gaacgggttc aggagatgga gaaaaagggg
     4021 atcgtgccga agagcggagc acataaatga gcaaggcagt gatctacaaa gggtacacaa
     4081 ttcaaccggc gctccgacat ctagttgata ccggccagtg ggaattgaat gtcttcattt
     4141 catggcaagc cgacgaggag gaggaaagtc gtcatttcta ttcgaccggt cggtatgcaa
     4201 cggaggacga agccacggcc caatgcattg catatggcca acagatcgtt gatggcaaga
     4261 ttcccggctc atcggttgga tgaagcggac gtgttagcca gctcctctcc atcttatgca
     4321 taggggcgtt gtcatgcata aagctggcga tcttaaccgt atccagggcc tgtatcgttc
     4381 cgagtgctgc ggcgttgagc agtcgctgtc agagaacgag aaatttcccc cctgtccagg
     4441 aagaggcaaa tcgggtgaga gtcgatgctc tggccagaat gccgcatgga tccttgtgcg
     4501 tccgacgtag tctagataac ccgttgctta ccccttcgat acagtctttg ctgggatgta
     4561 cctagatggg gatgtgtatc ccaacctcgt ggcttccgag ccggtctacg ccctgtcgaa
     4621 caatacaatc atttctcagc tcatgagcct tgtggtttaa gtttccctct cctagaatat
     4681 acctctccgt gaggccccca tgcttttc
//
LOCUS       DATIPF010000324         3373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_947637, whole genome shotgun sequence.
ACCESSION   DATIPF010000324 DATIPF010000000
VERSION     DATIPF010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947637"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /gene="mutL"
                     /locus_tag="VLL06_08110"
     CDS             complement(<1..365)
                     /gene="mutL"
                     /locus_tag="VLL06_08110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HSS30967.1"
                     /translation="MPAASCSAKIHVLPGDVVSRIAAGEVIERPAAVVKELLENSLDA
                     GGLHVTIDVKDGGMTLIRVTDDGEGISRADLPLAFERHATSKLRSDRDLALVTTMGFR
                     GEALPSIASVSHVLVTTAT"
     gene            complement(432..1211)
                     /gene="ybgF"
                     /locus_tag="VLL06_08115"
     CDS             complement(432..1211)
                     /gene="ybgF"
                     /locus_tag="VLL06_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HSS30968.1"
                     /translation="MRKKTGSRGLVVFLAGTMLWLAGCANHADFVELRDHLSTVSRSQ
                     DQDQKRLDALQRRLEALERVRDSESARPKLDPRFDELSARIQKLEARLAKSEEAAGIP
                     VVPKVEPILGEPARLPKPQKPTDGPTMMPGVPTITPTSAFNLAYNDYLNGKYELAVAG
                     FQRFVKDFPGTSLTPNAYYWLGESSYQQKDYVRAMQSFEYLAMEYPGNEKVPAALFKS
                     GLAAGETGDLAKSKKNLKRVIEEFPTSDEAKLAKNKLAEIR"
     gene            complement(1217..2671)
                     /gene="ybgF"
                     /locus_tag="VLL06_08120"
     CDS             complement(1217..2671)
                     /gene="ybgF"
                     /locus_tag="VLL06_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HSS30969.1"
                     /translation="MWNCSATKGTWATIGVICTVLLTGCVAQQADLKQTEKILQQRIK
                     QTSDESAQTRARQSQEISVLREQELPQLRGELERALHQTQELQGKQEDLKQRAAQLEQ
                     QTKRLEQLAGKLEADSTTRYNQVRESLNAQDLKNKQDRDQLRMDVNTRLDDVIRQMEV
                     LRKEIIDVVQKTNSGLAKSLDVRLEEQHKELTDNQVRIEQLSAKFAQFSQSLTAFRDS
                     LTGLNERVTQEEQESKASTIHINEMNKAVTGHLGEVNKSVASVAQKFAARLDEQDRRL
                     DTLSKTVDQVAQDGHVRGGNKGGTRQSTTKPAPRSSVVPSADSPKVELESASTSLPVQ
                     GETVASAVESEGSLRPQFSQSAERSDKLEYERLLTLFRDGDLDGARQGFASFLRDYEN
                     SELSPNARFWLGEAHYGKRDYRQAIDSYDRVELDFPRSEKVPAAILKKGFAYLAMKDK
                     KRAFSAFKQVVTSYPRSPEAGKASDKLSQMKESR"
     gene            complement(2759..>3373)
                     /gene="pal"
                     /locus_tag="VLL06_08125"
     CDS             complement(2759..>3373)
                     /gene="pal"
                     /locus_tag="VLL06_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HSS30970.1"
                     /translation="MPGCSKKSVQSGGDSQSTQGMAKGGSADNSRSGVGSFPDTGVQS
                     GGGGLRGLDKNPSEERVGGGTLLAKVDPSSSAERQMAEVRAEQAASAAAGLRDVFFTY
                     DSWTISEEGRQALSRDAEWMKSTPSAVVKVEGHCDERGTSAYNLVLGEKRAKAARNYL
                     VELGVSANRLSVVSYGKERPSCNEHAESCYQQNRRGHLALKTGK"
BASE COUNT          676 a         1034 c          852 g          811 t
ORIGIN      
        1 ctcgttgcgg tcgtcaccag cacatgggaa acggatgcaa tgctcggtaa tgcctcgccg
       61 cgaaagccca tcgtcgtcac caaggcgaga tcccgatcgg acctgagctt gctggtcgca
      121 tgccgctcga aggccagggg aaggtctgca cggctgatcc cctccccatc gtccgtgact
      181 cgaataaggg tcatgccacc atcttttaca tcgatcgtga catgcagacc accggcatcg
      241 agactgtttt ccagtaattc ttttacgacg gcggcaggac gctcgatgac ttcaccagcc
      301 gcgatgcggc tgacgacgtc gcctgggagg acgtggatct tcgcggagca actggcggcg
      361 ggcatgaaat tagactccag ggaacaccgg gaataaggaa gccatcctga cccatcccgg
      421 tggacggccg atcatcgaat ctcggccagc ttattcttag ccagtttcgc ctcgtccgac
      481 gtgggaaact cttcgatgac ccgtttgaga tttttcttcg atttggcgag atctccggtt
      541 tcccctgcgg cgagccccga tttaaagagg gcagccggca ccttctcgtt cccgggatac
      601 tccatcgcga ggtactcgaa cgattgcatc gcacgcacgt aatctttctg ttgataggaa
      661 gactctccca accagtaata ggcattcggt gtcagtgatg taccgggaaa atctttgaca
      721 aatcgctgaa aacccgccac ggcaagctcg tatttcccgt tgagatagtc gttgtatgcc
      781 agattgaagg ccgatgtggg ggtaatcgtg ggcacaccgg gcatcatcgt cggaccatcc
      841 gtcggcttct gcggtttggg aaggcgagca ggttctccta ggattggctc caccttgggc
      901 acgaccggga tacccgcggc ttcctcggat ttggccagcc gggcctctaa cttttggatc
      961 cgtgcagaca gttcatcgaa cctcggatca agctttggtc tggcggactc gctatccctt
     1021 acccgctcca atgcctctaa acgtcgttga agggcgtcca ggcgcttttg atcttgatct
     1081 tgagatctcg acaccgtgga taagtggtca cgcaactcaa caaagtccgc atggttggca
     1141 catccggcga gccacagcat cgtcccggct agaaacacaa ccaaccccct tgaacctgtt
     1201 tttttgcgca tcacggttat cgcgattcct tcatctggga tagtttatcg gacgcttttc
     1261 ctgcctctgg actccgtgga tacgatgtca ccacctgttt aaaggccgaa aatgcgcgct
     1321 tcttatcttt catcgccagg tatgcaaacc ctttcttcaa gattgccgcc ggcactttct
     1381 cgctccgggg gaaatccagt tcaacacggt catacgaatc aatcgcttgt cgataatctc
     1441 tcttgccgta atgcgcctca ccaagccaaa agcgcgcgtt gggagacaac tctgagttct
     1501 catagtcgcg caaaaatgag gcgaatccct gccgggctcc atccaagtct ccatcacgaa
     1561 acaacgtgag cagacgttcg tactcgagtt tatcggaacg ctccgcactc tgactgaatt
     1621 gcggtcgcag cgacccttcg gattcgaccg ccgacgccac cgtttcgcct tgcacaggga
     1681 gcgaagtcga agccgattcc agctcaacct ttggagaatc agccgaaggt accactgacg
     1741 atcgaggggc cggcttcgtg gtcgactgac gtgttccacc cttatttccc cctcgtacgt
     1801 gcccatcctg cgcgacctga tcaacggtct tagaaagggt gtccagacga cggtcctgct
     1861 catccaagcg ggccgcaaac ttttgcgcga cagatgccac actcttattg acctcgccta
     1921 agtgaccagt caccgccttg ttcatctcat tgatatggat ggtcgacgct ttactctcct
     1981 gctcttcttg ggtgacccgc tcgttcaatc ccgtcagcga atctctgaag gccgttaaag
     2041 actggctgaa ttgtgcgaat tttgccgaca actgttcaat ccgaacctgg ttatccgtca
     2101 gctccttatg ctgttcctca agacgtacgt caaggctctt ggcaagcccg ctgttcgtct
     2161 tctgcacgac gtcgatgatc tcttttcgaa ggacctccat ctggcggatc acgtcgtcaa
     2221 ggcgagtatt cacatccatg cggagttgat cccgatcctg cttattcttg agatcttgcg
     2281 cattgagact ctcgcgcacc tggttgtagc gagtcgtgct atccgcctcc agcttgccgg
     2341 ccaactgttc aagccgcttg gtttgttgct caagttgcgc cgcgcgctgt ttgaggtctt
     2401 cctgtttgcc ctgaagttcc tgtgtttggt gcagcgcccg ttccaattcg ccacgcaact
     2461 gcggcaactc ctgttcccga agcaccgaaa tctcctgact ctggcgtgca cgggtctgtg
     2521 ccgactcatc gcttgtttgt ttgatgcgct gctgtagaat cttttccgtc tgtttgagat
     2581 ccgcctgttg agcaacgcat ccggtcaaca gcacggtgca gattacaccg atcgtcgccc
     2641 aggttccttt tgtcgccgaa caattccaca ttgttccacc cacaatgata aaaggaatcc
     2701 tttaccggct ctgtgctgac aacggagtca cagtcgccac ggatacagcg cgcgtcgcct
     2761 acttcccggt cttcaatgca agatgtcctc gccgattctg ctggtagcac gattccgcat
     2821 gttcattgca ggacggccgc tctttcccat acgacaccac ggatagacga ttggcactga
     2881 caccgagttc gaccagatag ttccgcgcgg ctttcgcacg tttttcaccg agcacgaggt
     2941 tgtaggcgga agtcccccgc tcatcacagt gcccttcgac cttcaccact gcactcgggg
     3001 tagacttcat ccactcagca tcgcgactca atgcctgccg cccctcctcg gaaattgtcc
     3061 agctatcgta ggtaaaaaac acatctcgca agcctgctgc agctgaagcc gcctgctcgg
     3121 ccctgacctc agccatttgt cgttcggcgc tgctggaagg atccaccttc gccaacagcg
     3181 tgccaccacc tacacgctct tctgaagggt tcttgtcgag gccccgaagc ccgccgccgc
     3241 cagactggac gccggtgtct gggaagctgc cgacaccaga tctagaatta tcggcactcc
     3301 cccccttcgc catcccttga gtggactggg agtcgccgcc agattgcacg gattttttgg
     3361 agcaaccagg cat
//
LOCUS       DATIPF010000325         2374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_594730, whole genome shotgun sequence.
ACCESSION   DATIPF010000325 DATIPF010000000
VERSION     DATIPF010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594730"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VLL06_08130"
     CDS             complement(<1..443)
                     /locus_tag="VLL06_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30971.1"
                     /translation="MLFAGLFAGTLCLGLILGDWLYFMRLTPDASRYGCGISRSHDRF
                     THTTLKQLAERFDAAGFLMLPHGTARLYQDLNQIVIRQKYRLFAMGFRTLWPLKGLIF
                     LSPEGDALSVLCRKLTPWSSALLTGFWFILVTVGTVGALISLFVEG"
     gene            676..1407
                     /locus_tag="VLL06_08135"
     CDS             676..1407
                     /locus_tag="VLL06_08135"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSS30972.1"
                     /translation="MELAKIERVYTSYAKVYDRVFGKVFHEGRQSIIKNLNVQCDEKI
                     LEVGVGTGLALPMYPRHCQIVGIDFSEGMLDQAKQRAAEHRMDHVQLHRMDAGAMEFK
                     DDSFDTVVAAYVVTAVPDYRKVVSEMIRVCRPGGRIIMLNHFSNDNKVIAAMEKVLSP
                     ITKHLGWRTDLALETVLEGTPLHVSRKQHVNPFRLWALVECVNEKYVNGKTHINGVKN
                     GNGAPAFAHINGTHLPKHTPGEALV"
     gene            complement(1501..2319)
                     /locus_tag="VLL06_08140"
     CDS             complement(1501..2319)
                     /locus_tag="VLL06_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30973.1"
                     /translation="MKYQVANIEDLRWLLGHTGGFRGGYVTDVQVSKRRLLDEASGRE
                     VPAGTTVTVVIRYHIRQMARVAKITMTGVTDFSMFEQEGADCSTLGVIQAELTDGRLR
                     FWFDPQGELYVVCEEAQLEEVAAPSLEPLSLEQVAQWTFQSTMPDWPTVTWVLAELDV
                     AGVPCIWRAIASSPGRHSAIQWEGELLPASMQGAVDARGVHCMLYGPHEGPGFGMVLR
                     VRGVQDRRTGQVLSLLADLVAQRFSGQCLVGNTIIPGGEWQNWKSFGQSHGSEE"
BASE COUNT          585 a          723 c          606 g          460 t
ORIGIN      
        1 ccttcgacga acagggatat cagagccccc accgtaccga cggtcaccaa gataaaccag
       61 aacccggtaa gaagggctga agaccaggga gtgagctttc ggcagagcac cgaaagtgcg
      121 tcaccttcgg gagagaggaa gatcaatccc tttaacggcc acaatgttcg gaagcccatg
      181 gcaaacagcc ggtacttctg acgaatcaca atctggttca gatcctgata cagccgcgca
      241 gttccatgcg gcaacatcag gaaaccagcc gcatcaaacc gttcagccag ttgcttcagg
      301 gtcgtatgcg taaatcgatc gtggctgcgg gaaatcccgc atccataccg actcgcatca
      361 ggcgtgaggc gcatgaagta gagccagtct ccgagaatga gaccgagaca cagcgtaccg
      421 gcgaacaaac cagcgaacag catagtggta cctatcccat aggggccgca ggtgaatcaa
      481 gcgacaggac cgagataatc tgttgactca ttgcagcgag ttggctagag tagcgcgagt
      541 gctattcagg cgggtcccct cgctccattc aggtgctccg ccgcacattt ttccccctcg
      601 gtcattgcgg cagtcggctc atccctcgcg ggtcgaggag cgcaagtcgc ctttaataat
      661 ggagtcggag gtgatatgga attagcgaag atcgagcgtg tctatacgtc ctacgccaag
      721 gtttacgatc gggttttcgg aaaggtcttt cacgaagggc gtcagtcgat catcaagaat
      781 ttgaacgtcc agtgtgatga aaaaatcctc gaagtcggag ttggaaccgg actggccctg
      841 ccgatgtatc cccgccattg ccaaatcgtc ggaatcgatt tttcagaagg catgctggac
      901 caagcgaaac agcgagccgc agaacaccgg atggaccatg tgcaactcca tcgaatggat
      961 gccggcgcga tggaattcaa ggacgatagt tttgacaccg tcgtcgcggc ctacgtcgtc
     1021 accgctgtcc ccgactaccg caaggtcgtc agcgagatga ttcgcgtctg ccgaccgggc
     1081 ggacggatca tcatgctgaa tcacttcagc aacgacaata aggtcatcgc cgccatggaa
     1141 aaagtgttat cccctatcac caagcactta gggtggcgga cagacctggc actcgaaacc
     1201 gtgctggaag ggacgccgct gcacgtctca cggaagcaac atgtgaatcc gtttcgcctc
     1261 tgggcgttgg tggaatgtgt gaacgagaaa tacgtgaacg gcaagacgca catcaacggc
     1321 gtgaaaaatg gaaatggcgc ccccgccttc gcacatatca acggcacaca tctaccgaaa
     1381 cacacccctg gcgaggcctt ggtctaagag cctgaccgat attgacctat tctaaacaat
     1441 ccgcaaccat ctgcatcgtt ctcgcccaga gacactcttc aatgtgttcc agtgaagacg
     1501 ctactcttca gacccgtgtg actgtccgaa tgacttccaa ttctgccatt ctccgccggg
     1561 aatgatcgta ttgcccacca gacattggcc ggaaaacctt tgggcgacca aatccgccaa
     1621 gagcgagagt acctgaccgg tacggcgatc ctgcacccca cgaacccgca gtaccatacc
     1681 gaatcccggt ccttcatgcg gtccatacaa catacaatgc acccctctcg catcgacggc
     1741 accctgcatc gacgccggga gcaattcccc ttcccactga atggcagagt gacgcccggg
     1801 ggacgaggcg atggcccgcc agatacaggg aacacctgcc acgtccagct cggccaatac
     1861 ccacgtcact gttggccagt ccggcatcgt actttgaaat gtccattgag ccacctgctc
     1921 taacgagagg ggttcgagac tcggcgcggc cacttcctcc agctgcgctt cctcgcacac
     1981 cacatagagt tcgccctgcg gatcaaacca gaaacgcagt cttccatcgg tgagttccgc
     2041 ttggatcacc cccagtgtcg agcaatcggc cccttcctgt tcaaacatcg aaaagtccgt
     2101 cacaccggtc atcgtgattt tcgccacacg agccatctgg cgaatgtgat agcggatcac
     2161 aaccgtgacc gtcgtccctg ccggcacctc ccggcctgac gcttcgtcta acagccgtcg
     2221 tttcgacacc tgcacatccg tcacatagcc accacgaaac ccgccggtat gaccgagcag
     2281 ccagcgcaga tcttcgatat ttgcaacctg atatttcatg gcatcaacac tcgcgaactc
     2341 cgaacgatct tagccgactg cggatcaact acct
//
LOCUS       DATIPF010000326         4542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_595113, whole genome shotgun sequence.
ACCESSION   DATIPF010000326 DATIPF010000000
VERSION     DATIPF010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595113"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1950)
                     /locus_tag="VLL06_08145"
     CDS             complement(<1..1950)
                     /locus_tag="VLL06_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS30974.1"
                     /translation="MIVCVLAQGLALHVASAVEPRESPAIPTATHAKPPQASAGTSLP
                     RNALPDDGPRYDRTVEGSEYLQFKEAVEAFTRGEYVVARSGFDALATLDSSSALIPPL
                     KAFLAELTVIEDPTDYGRREAITQYRSIIGSYPKDTNAFRALWRVGDLYVEIGWFQEA
                     LVAYEYALSRELPRHDADRSMLALGATLGKLGRWTEAERAFDTVRKRATEDRLAARAT
                     LDQADALYVQRRKQDAVPLYDFLYRRWPDLLKTNPDALQQYGDVLFAAQELRRARDID
                     MLLYNLFPLHRHAGTALVRLGDGQSRLGLRTPAELFYTAAKTQYADTAAAVVARMRLS
                     QIEQEIAASAGQDFLHKKVEGMIRGAGISYLEPSEGEEIHKAIAREHRDDMLGSEALF
                     HLAEQYELHGNPARAIQVFQDITRRTGIIPYDPWPQAAGLHLASILKPQLESAIASKK
                     DVQVLTLFHSHGQDPEQYYVGTRLLLEVADTHRRLGFSTEAIHLYQTFMRNRKVAVLH
                     EAALIGLGESYLDQSDPLAARNVFESFRLQYPRSPRTSRVSKQLATAMLEQGDRQSAV
                     RVMRQWAKLHPRDTSHNWIYVTLGRTLAEDRKREEAVAAFEDAVRHDALRTPQDFLLY
                     ADLLMKMNKPERAAELYSQVVRAWPG"
     gene            complement(2410..3819)
                     /locus_tag="VLL06_08150"
     CDS             complement(2410..3819)
                     /locus_tag="VLL06_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSS30975.1"
                     /translation="MSVLSILVVEDDEGLRNLLRRLLSQEGRLVTVVGSGQEAIRLAQ
                     EIPVHVLVTDLQLPDIDGLAVLERILQIDPKVIGVVMTGYGSIDCAVKAMKAGAFEFL
                     TKPFENEVVVGSIDKAVEAHRRRESRQSSRKAIREQYRLEQFVGTSEPIQRVLEFVAK
                     VADCDSTVLIHGESGTGKELVARMLHFNSEPKDRPLVPVNCGAIPENLLESELFGHEK
                     GAFTGAAHTRIGRFESAHGGTIFLDEIGELSLGLQVKLLRVLQERSFERVGGTRTIDV
                     DVRVVAATNQDLALAVQQKRFREDLYYRLNVIPVTIPPLRERRSDIPQLVNHFLGKMA
                     RGRQASTVGCSPEAMTRLMEYHWPGNIRELENMIERLVVLNRSGTIEVSDLPEHLQRR
                     PVSTDQSEAHFISFSDQGVNLSRELEQLENRLIVGALRQANGITSKAAQLLNVNRTTL
                     VEKLKRKGFATKTQGCPVF"
     gene            complement(4326..>4542)
                     /locus_tag="VLL06_08155"
     CDS             complement(4326..>4542)
                     /locus_tag="VLL06_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HSS30976.1"
                     /translation="LVAEGKTNKEIGSSLELSDKTVKNYIRFIFQKLKVTRRAQAAAF
                     FIRDSPSERLSDLQPSLRMDKKPVKSR"
BASE COUNT         1011 a         1299 c         1203 g         1029 t
ORIGIN      
        1 gccgggccag gccctgacta cctggctgta gagttccgcg gcacgttcgg gtttattcat
       61 cttcatgagc agatctgcgt acagcagaaa atcttgtggt gtgcggagcg cgtcgtgtcg
      121 cacagcatcc tcgaatgccg cgacggcctc ttctcgcttc cggtcttccg ccaacgtacg
      181 tccaagcgtg acgtagatcc aattgtgtga cgtatcacgc gggtgtaact tcgcccactg
      241 ccgcatcacc ctgacggcac tctggcgatc tccctgctca agcatggccg tcgccaactg
      301 cttcgagact cggcttgtcc gtggactccg ggggtactga agacggaaac tttcgaacac
      361 gttccgagca gccagaggat cagactggtc caggtaactt tcgcccaaac cgatgagggc
      421 cgcttcgtgg agcacggcca cctttcgatt tcgcatgaag gtttgataga ggtgtatcgc
      481 ttcggttgag aagcccagcc ggcgatgcgt atcggccacc tccagtaata ggcgtgtgcc
      541 gacatagtac tgctccggat cctgtccatg gctgtgaaac agtgtgagca cctggacatc
      601 cttcttactc gcgatggccg actccaactg aggtttcaag atggaggcca ggtggagtcc
      661 cgcagcctgc ggccaaggat catatgggat gataccggtc cgccgagtga tgtcttgaaa
      721 gacctggatc gctcgcgcag ggtttccatg caattcgtac tgctctgcca aatgaaacag
      781 cgcctcgctg ccaagcatgt catcccgatg ttctcgggcg atggccttat ggatctcctc
      841 gccttcggat ggctccagat aggagatgcc ggcccctcgg atcatgcctt ccaccttttt
      901 gtgcaagaaa tcctgtcctg ccgacgccgc aatttcttgt tcgatttgtg acaggcgcat
      961 gcgagcgacg accgctgctg ctgtatcggc atactgcgtc ttggcggcag tataaaaaag
     1021 ctctgccggc gtgcgcagac cgagtcgact ctggccgtcc ccaaggcgga cgagtgccgt
     1081 acccgcgtgc cgatgcaacg gaaagaggtt gtacaggagc atgtcgatat cgcgcgctct
     1141 tctcagctcc tgcgcggcaa aaagaacatc gccatactgc tgcaatgcat cgggattcgt
     1201 tttgaggaga tccggccagc gccggtacag aaaatcatac agcggtactg cgtcttgctt
     1261 gcgcctctgg acatacagag cgtcggcctg atccaacgtc gccctcgcgg caagtcgatc
     1321 ctcagtcgcg cgttttcgga ccgtatcgaa ggcccgttcc gcttctgtcc accgccccaa
     1381 ctttccaagt gtggccccca gagcaagcat ggatcggtcc gcatcatgac gaggtagctc
     1441 gcgggaaagc gcatactcgt aggcgacaag ggcttcctgg aaccatccta tttcgacata
     1501 caggtcaccg acccgccaga gtgccctgaa tgcattggta tctttcggat acgagccaat
     1561 gatgctgcga tattgtgtaa tcgcttctct ccgaccatag tcggtcggat cctcgatcac
     1621 ggtgagttcg gccaagaagg ccttcaacgg tgggatcaat gcgctggaag aatcgagtgt
     1681 ggcaagagcg tcaaagcctg agcgggcgac aacatattcg ccccgggtga atgcctctac
     1741 cgcttctttg aattgtagat actccgaccc ttccacggtt cgatcatagc ggggcccatc
     1801 atcgggcaga gcattccgtg gcaaggacgt ccccgcactc gcctgaggtg gcttcgcatg
     1861 agtggctgtt ggtatggccg gcgactcccg tggctcaacc gcagatgcga catgaagcgc
     1921 gagaccctgc gcaagcacac acacgatcac gccaacacat gatgcatgca ttctcataga
     1981 cacgtcagga gttcagcaat ttctgtgccc tttgtattgg atgaataaat gctgccggta
     2041 tgagagaagt ggcaactcgg cacgcgatga ttcggcaaaa catgtcaatc gtccgacgcg
     2101 gcgctcatat gttcgtcaat caattgacgg aatgcgcacc gaaatattcg atgcgaactg
     2161 gtcaaggggc tatcggaaca gagtcacggg aacgcccggt catcctggaa ggttgcatat
     2221 gaacaggcag gggaaagccc tgtatacgcg aaaatggcgg ggcgggcggg actggcccgg
     2281 ttgtctcgtt gatctggttt atttgatctg tttagttcat ctagttggtc tcattcaacc
     2341 aaacaaacga gaaaaaccag acaaaccaac caacggtctt ctaggccggc agactgtttc
     2401 agcactctgc tagaagaccg gacagccctg agtcttcgtt gcaaaccctt ttcgtttcaa
     2461 cttctcaact agggtcgtgc ggttcacatt gagtagctgc gccgccttgc tcgtaatccc
     2521 attggcttgt cgaagcgcac caacgatgag acggttctcc agctgctcga gctctcgtga
     2581 cagattgact ccctgatcgc taaaggagat gaaatgtgcc tcggactgat cggtagatac
     2641 cggacggcgc tggaggtgtt cgggaagatc ggacacttcg atcgttcctg atcggttcaa
     2701 caccacgaga cgctcgatca tgttttccaa ttcacgaata tttccaggcc agtgatattc
     2761 catgagacgg gtcattgcct ccggagagca gcctaccgtg ctcgcctgcc tgccccgagc
     2821 cattttcccc aagaaatgat tgactaattg cggaatatca cttcgccgct ctctcagcgg
     2881 tggaatggtg acagggatca cgttcagccg atagtacaga tcctcccgga agcgtttctg
     2941 ctgcacggcc agcgccagat cctgattggt cgctgccacg acacggacat ccacgtcgat
     3001 cgttcgagtc ccccctaccc gttcgaacga ccgctcctgc agcacccgaa ggagcttcac
     3061 ttgtagcccc agactcaact caccgatttc gtccaggaaa attgtcccgc catgtgccga
     3121 ctcgaagcgg cctattcgcg tatgcgctgc gccggtgaag gctcccttct cgtgtccaaa
     3181 caactccgat tcgagaagat tctctggaat ggcgccacaa ttaaccggaa cgagcgggcg
     3241 atcttttggc tcgctgttaa aatgcagcat ccgcgcaacc aactccttgc cggtcccgct
     3301 ctctccgtga atcagaacgg tactgtcgca gtccgcgacc ttcgcgacaa attccagcac
     3361 gcgctggatc ggttcgctcg tcccgacgaa ctgctccaac cgatattgtt cgcggatcgc
     3421 ttttcgcgac gactgtctgc tctcgcgacg gcgatgcgcc tccacagcct tgtcgatgga
     3481 gccgacgaca acttcgttct cgaatggttt ggtgagaaac tcaaaggccc cggccttcat
     3541 ggctttgacg gcacagtcga tcgagccata gccggtcatt acaaccccga tgacttttgg
     3601 atcgatctga agaatgcgct ccaggaccgc aagtccatcg atatcaggca actgcagatc
     3661 cgtcaccagc acatgaacag gaatctcttg ggccagacgt atagcctcct gtccgctccc
     3721 gacaaccgta actagacggc cttcctgaga caaaagcctc cgaagcaggt tccgcagccc
     3781 ttcatcatct tcgaccacaa gaatagacaa cacgctcata cggtgttcct cataatattg
     3841 gtggcacatg catatgaatc aatacgggca ccgagaatcc ctgcgattgc gggattggca
     3901 ggaaccatcc tgatcgatct cggctttcat gaaatatgcg cttcccatcg atacaaagcg
     3961 tataagagtt cttaaaatca gaaagtgcct aatggctctc ataaatttgg catttagagc
     4021 ccattgaaga atgcacatat gggagctgac atgctctatt gtgccctata ccatggacta
     4081 cagttcctgt aagcctcctg agcgagttct cactctcggt gtgccgtaag gttcacccct
     4141 gcatcaacag aagtgccctc tgccgaagcc atatggcacc aggaagactt ccataacgag
     4201 gaagatgcat gcccgatgat gagattggcg gagactgaga gctaacttct tgaacgaata
     4261 ggaacaggcg atgcgcggaa tagttactct ggtgtgaggg agtggaactt cccgattggt
     4321 gtccgttacc gagatttcac tggtttttta tccatcctca acgacggttg caagtctgac
     4381 agacgttccg agggagagtc gcgaataaaa aaagccgcag cttgggcgcg ccgggtgact
     4441 ttcagttttt gaaaaatgaa tcggatatag ttcttcacgg tcttatcgct gagttcaagc
     4501 gatgagccga tttccttgtt cgtcttcccc tcggcaacga gc
//
LOCUS       DATIPF010000327         5256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_948307, whole genome shotgun sequence.
ACCESSION   DATIPF010000327 DATIPF010000000
VERSION     DATIPF010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948307"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VLL06_08160"
     CDS             <1..478
                     /locus_tag="VLL06_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30977.1"
                     /translation="FGKDILMLGGTVIGFIAAMLSVIEKLLDFRNRMAPKKERKSPSP
                     SERPFSDPAPAPAIDFFSSKPFRGVSYLLLYETSVIIAAGLILNYIGLTLSRHLESIL
                     FLDMTGTALVAFLLGPWWGAIAALLSNSLVNWLLYPETGADVVIFPWSLVSMTGAC"
     gene            488..1354
                     /locus_tag="VLL06_08165"
     CDS             488..1354
                     /locus_tag="VLL06_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30978.1"
                     /translation="MARQAGFQKYLRTGRSSALSHAWFLYIFGVGSAIVMSLPGTFIQ
                     AALNEQTTYALNPAVAETVSLRVLQWEQTVHLYLESLFGFTWGESVSWWMVNWFQNWI
                     RYIPDKTMSAAIALVVLKYGYPLFERELIHGGPAGERPTDERILPLVLGLLYALPFAA
                     LLSSDTYGGAAYWPLWALPWLFIVAGYVYLRYWGTGEAALQVSRLQRADRYARALKQI
                     GREASHEFCKRLTFMTLIVSLVFALCLPILLTDFYRATFKFFCVVYGFLLVVHLVRIS
                     IAQNISVARANE"
     gene            complement(1418..2281)
                     /locus_tag="VLL06_08170"
     CDS             complement(1418..2281)
                     /locus_tag="VLL06_08170"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSS30979.1"
                     /translation="MEKEPPSPRAEFLIAMAGPLVSLTLGAACLGGALAVEYVLAQPG
                     FQGLVVLASLLGMVNVQLGLFNLIPGFPLDGGRVLRAGLWAWNKDFNRATSQAALAGL
                     GFGMGLGLIGAVLMVGVWSGALGESIATNGGWLIFIGTFLFSAALSSRRQAAQRTSLT
                     SVTVRQVMVHRVVTLPPDMSVQDAVDQYFVVHGFGGFPICSEGQVLGVLTVQDVQALP
                     TGLWPWRLVREVMRPTSPAFCIHPDWSIMQAMELMAQGEWDRLVVLENGEIVGLITRS
                     AITRFLQLHKA"
     gene            complement(2351..2650)
                     /locus_tag="VLL06_08175"
     CDS             complement(2351..2650)
                     /locus_tag="VLL06_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30980.1"
                     /translation="MLADFFSTLLGGKAELTLQFPHWYGGSMSLPNWRIGRALGIPIH
                     VHASWFVVFFFVTWSLAVGYLPGTLPDLSVPRYWGMGGVAAILLFLSVLLHELGH"
     gene            2847..3563
                     /locus_tag="VLL06_08180"
     CDS             2847..3563
                     /locus_tag="VLL06_08180"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HSS30981.1"
                     /translation="MQSHYLPGMLGLVLTMLLVPAIPTFTQAQVIKSGPSSCPGVALT
                     FDLCPVRNGSGYDQALIDYLIEQKIPATFFMSGKWITKHDQQVRALLQVPFFEVGTHG
                     EVHAHLPLHSADEQKQEILGPVRLLKAKYGHDATLFRPPYGEFNDDTVNVARALGLQF
                     ILWDVVSGDPDPTLTAIRIEDRLKRLVRKGSVIVMHANGKGRHTHEVVQDLHQQLLPQ
                     RNLTPMTMSDLLICNGQAVP"
     gene            3560..4033
                     /locus_tag="VLL06_08185"
     CDS             3560..4033
                     /locus_tag="VLL06_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSS30982.1"
                     /translation="MTDPIVRPMTVEDRTAVIELLTDSDPWKRLGYRASDWDDYFTPL
                     PQGRDSYVVDQNGRAAGIGVVRQKFLLGDYVELFGVAAWARDKGLGRLLLAHIEAVVF
                     ARAKNLFACVSDFNDHARHFYKKQGYLEIGPMPNFLIPGSAEILLRKTSGPARGK"
     gene            4099..4497
                     /locus_tag="VLL06_08190"
     CDS             4099..4497
                     /locus_tag="VLL06_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSS30983.1"
                     /translation="MQVKKLLHTRMRVSDMEQTIAFYTNVLGLEVLERKVSPRGSHLA
                     FLKVPNSEELIELTSFPPSGPVKVQEDLVHLAFQVESLDDTIASLNAKGVKVTDGPTQ
                     TSSGSRFIFIDAPDGYEVELIERPAGVKIV"
     gene            4581..>5256
                     /gene="dapF"
                     /locus_tag="VLL06_08195"
     CDS             4581..>5256
                     /gene="dapF"
                     /locus_tag="VLL06_08195"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HSS30984.1"
                     /translation="MKNGFFRGHGLGNDYIVMDPKELSFSLTPSRIKAICDRNWGLGS
                     DGILALVPSKKADFGLRIFNPDGSEAEKSGNGLRIFARYLHATGKTKKQHFTVETKGG
                     LVTIAIHVDRYGDASAVTVEMGRATFQPGALPCTLMINELIEQPIDAAGQSLTFTGVS
                     VGNPHCVVFKPVGQSWSREDLLKLGPALENHALFPRRTNVQLAVPTGPKELFILIWER
                     GAGETQA"
BASE COUNT         1173 a         1568 c         1387 g         1128 t
ORIGIN      
        1 cttcgggaaa gacatactca tgctcggcgg cacggtgatt ggcttcatcg cggccatgtt
       61 gtctgtcatc gagaaactgc tggatttccg gaaccggatg gcaccgaaga aggaaagaaa
      121 atcaccttcc ccaagcgaac gtccgttctc cgacccagca cccgctcctg caatcgattt
      181 cttttcctcg aaacccttcc gcggcgtctc ctatctcctc ttgtatgaaa ccagcgtcat
      241 catcgcagca ggcctgattc tcaattacat tggactcaca ctcagtcgtc atctggaaag
      301 cattctcttt ctcgatatga cgggaacagc actggttgcg ttccttctgg gaccctggtg
      361 gggagccatc gccgccctcc tctcgaattc tctggtgaac tggttactgt acccggaaac
      421 cggagcggat gtggtaattt ttccttggtc gctggtgagt atgaccggcg cctgttagtg
      481 gggttggatg gcccggcaag cagggttcca aaagtatctc cgcaccggac gcagttccgc
      541 cctgtctcat gcctggttcc tctatatctt tggcgtcggt agcgcgatcg tcatgagcct
      601 gccgggaacc tttatccagg cggcactcaa tgagcaaacg acctatgcct taaaccccgc
      661 cgttgccgaa acagtgagcc tacgcgtgct ccaatgggaa cagacggtgc atctctatct
      721 cgaatccctt ttcgggttca cctgggggga aagtgtgagt tggtggatgg tgaattggtt
      781 ccagaactgg attcgctaca ttcctgacaa aaccatgagc gcggccatcg cgctcgtggt
      841 cctcaagtat ggctatcctc tgtttgaacg ggaactgatt catggaggac cggctggcga
      901 acgtcccact gatgaacgca tcctgccgct ggtattggga ttgctttatg ccctcccttt
      961 cgccgccttg ctcagcagcg acacctatgg aggagccgcc tattggcctc tgtgggcatt
     1021 gccctggctg tttattgtgg ctggctatgt ctacctacgg tactggggaa caggtgaggc
     1081 tgccttgcag gtatccagac ttcaacgagc cgaccgttat gcccgagcgc tgaaacaaat
     1141 cggacgggaa gcctcccatg agttctgcaa gcgattgacc ttcatgaccc tgatcgtcag
     1201 cctggttttc gccctctgtc tccccattct tttgacagac ttttatcgtg ccaccttcaa
     1261 gttcttctgc gtcgtctatg gatttctgct cgtggtccat ctcgtacgta tctccatcgc
     1321 ccaaaacatt tccgtcgcgc gcgcaaatga gtgaccgtaa gatatggtgg gagaggcagg
     1381 gggtgaaaag actatcccct ttcaatcgct tgaggcctca agctttgtgc aactggagga
     1441 agcgagtgat agccgatcgg gtgatgagcc ctacgatttc tccattctcc agcaccacga
     1501 gacgatccca ctcaccctga gccatgagct ccatcgcctg catgatggac cagtccggat
     1561 ggatacagaa ggccggcgac gttgggcgca tgacctcgcg aacgagacgc cacggccaga
     1621 gtcctgtagg aagcgcttga acgtcctgca ccgtgagcac cccgagaacc tgcccttcac
     1681 tgcatattgg aaatccacca aatccatgca cgacaaagta ctgatcgacc gcgtcctgca
     1741 cgctcatatc cggaggcaat gtcacgacgc ggtggaccat gacttggcga acggtcaccg
     1801 acgtcaacga cgtcctctgg gccgcctgcc gtctgcttga cagagctgct gaaaagagga
     1861 atgtaccgat aaagatgagc cagcctccgt tcgtcgcgat agattctcct agcgcaccgg
     1921 accagacccc caccatcaag actgcaccaa tcaacccgag acccatacca aatcccagtc
     1981 cagcgagcgc ggcttgactt gtcgctcggt tgaaatcctt gttccaggcc cagagcccgg
     2041 cccgtaacac acgtccgccg tctaacggga acccgggaat caaattgaaa agtccgagct
     2101 gtacattcac catgccgagc aaactcgcga ggacgacgag tccttgaaac ccgggttgcg
     2161 ctaaaacata ttccactgct agggctccgc cgagacatgc agccccgagg gtcaaactca
     2221 cgagcggtcc tgccatcgca atcaaaaatt ccgctcgtgg actgggtggt tccttttcca
     2281 tatgtgccac ccctccgaaa ataaacaatg tgatctgccg gatcgggatg tggtagcgca
     2341 gcgccacata ctaatgcccc aactcatgga gcaggacgga gaggaacaac aagatggcgg
     2401 caacgccacc cataccccaa tagcgtggaa ccgacagatc gggcaacgtt cccggcaaat
     2461 accccaccgc caatgaccag gtcacaaaga agaacacgac aaaccaagag gcatgcacat
     2521 gaatcgggat gccgagcgca cgaccgattc tccaattggg taaagacata gatccaccgt
     2581 accagtgcgg gaattgaagc gtcaactcgg ctttgcctcc tagcagggtg ctgaaaaagt
     2641 ccgccagcat cgttctcgca tcgttcagat cctcaacgta ccccagaggg tacgcctccg
     2701 gtcttcactc gctgcggcct tgctggacga actttttgag cagcctgcga gttatcgctt
     2761 ccctgcccaa aacacatagg gtaccgagcg gctggcatac cacaatccgt ttttgcgaag
     2821 ccttcggatc gggtatactc atcaccatgc aatctcacta cttgccaggg atgctgggcc
     2881 tcgtcctgac gatgctgctg gtcccagcga tccctacctt cacacaggcg caggtcatca
     2941 agtcaggccc gtcttcctgt cccggagtcg cgttgacatt cgacctttgc ccggtgcgca
     3001 acggatcggg ctacgatcag gcgctgatcg actatttaat cgaacagaaa attcccgcca
     3061 ccttctttat gtccgggaaa tggatcacga aacacgatca gcaagtacgg gctctgctcc
     3121 aggtcccgtt tttcgaagtc ggcacccatg gggaggtcca cgcccatctt ccgctccatt
     3181 cagccgatga gcagaagcaa gaaattctcg gaccggttcg tctgctcaaa gccaagtatg
     3241 gtcatgacgc gacgttgttt cgtcctccct atggggaatt caacgacgac acggtcaacg
     3301 tggcccgagc ccttggcctc cagttcattc tctgggatgt cgtgtccggc gatcccgatc
     3361 cgacactcac cgccatccgg atcgaagacc ggctcaaacg gttggtcagg aagggcagcg
     3421 tcattgtgat gcatgccaac gggaaaggca gacatacaca cgaggtcgtg caagacctgc
     3481 atcagcagct cctgccccaa cggaatctca ctcctatgac catgagcgat ctcctaatct
     3541 gtaatggcca ggcagtccca tgaccgatcc gattgttcgg cccatgacgg tggaggatcg
     3601 aaccgccgtg atcgagttac ttaccgattc cgatccgtgg aagcgactgg gctacagggc
     3661 cagtgactgg gacgactatt tcacacccct cccacagggg cgtgatagtt acgtcgttga
     3721 ccagaatggc agggcggccg gcatcggagt cgttcgccag aaattcctgc tcggcgacta
     3781 tgtagaattg tttggggtgg cggcctgggc aagggacaag gggctaggaa ggttgttgct
     3841 cgcccatatc gaagcagtgg tcttcgccag ggcgaagaac ctcttcgcct gcgtatcgga
     3901 tttcaacgac cacgcccggc acttttacaa gaagcagggc tatctggaga tcggtcccat
     3961 gccgaacttt ctgatcccag gcagtgcaga aattctgctg cgcaagacca gcggcccagc
     4021 gaggggtaag taaaagtggt agttacttgt ataccgctgt tgccctttcc ctttgaactg
     4081 tttccttagc aggaacctat gcaagtcaag aaactcctcc acactcgtat gcgcgtcagc
     4141 gacatggagc agacgattgc cttctacacc aacgtccttg gattagaggt gctggaacgg
     4201 aaggtctcac cgcgcggctc gcacttggcg ttcctcaagg tgcctaacag cgaggagctg
     4261 atagagctga ccagttttcc tccgagcgga ccagtgaaag tccaggaaga tcttgtccat
     4321 ctcgcgtttc aggtggagag cctcgacgac accatcgcgt cgcttaacgc gaagggagtc
     4381 aaggtcacag acggacccac gcaaacatcc tccggcagcc gcttcatttt tatcgatgcc
     4441 ccggacggtt atgaagtcga actcatcgaa cgcccggccg gcgtcaagat cgtgtaagat
     4501 cccttcacat ccctctttcc ctccccacac cgttcatgct aggtatctct ccctgtctcc
     4561 ctctattcaa ggaactgcgc atgaaaaacg ggttctttcg cgggcatggg ctgggcaatg
     4621 actatattgt aatggacccc aaagaactgt cgttctcgct caccccgtca aggatcaaag
     4681 ccatctgtga tcgtaactgg ggtctgggta gcgatgggat ccttgcgctg gtcccgtcaa
     4741 agaaagcgga cttcggcctg cgcatcttca accctgacgg cagcgaagcc gaaaaatccg
     4801 gtaacggcct gcgtatcttt gcccgctatc ttcacgcgac cggcaaaacg aaaaaacagc
     4861 acttcaccgt cgagaccaaa ggcgggctgg tgaccattgc gatacatgtg gaccggtatg
     4921 gggatgccag cgccgtcacc gtcgaaatgg gccgggccac gttccagccg ggggcgctcc
     4981 cctgcaccct catgatcaac gaactgatcg aacagcccat cgacgcagca ggccaatccc
     5041 tcacctttac cggcgtgagt gtggggaatc cccactgtgt tgtcttcaag ccagtcgggc
     5101 agtcctggtc gagagaggat ctcttgaaac tagggccggc actggagaac catgcgctct
     5161 tcccgaggcg aacgaacgtt caactcgccg tgccgacagg tcctaaagag ctctttattc
     5221 tgatctggga acgtggggcc ggcgaaaccc aggcag
//
LOCUS       DATIPF010000328         5537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1036092, whole genome shotgun sequence.
ACCESSION   DATIPF010000328 DATIPF010000000
VERSION     DATIPF010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036092"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VLL06_08200"
     CDS             complement(<1..399)
                     /locus_tag="VLL06_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HSS30985.1"
                     /translation="MMTNLTQTPVRVSWLDRMLITAVVLLSSVFPVELFSNTSPAPRG
                     SDGQYSGRQGQVLVVKVKGEERATEVKGTFLNRTIPFFREFRSEEPPGYIGLLGIDMQ
                     DDPGTYELTVEVKQGEQTKHLSFNVLVAKEK"
     gene            complement(396..1790)
                     /gene="pafA"
                     /locus_tag="VLL06_08205"
     CDS             complement(396..1790)
                     /gene="pafA"
                     /locus_tag="VLL06_08205"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HSS30986.1"
                     /translation="MLQPIPLKRRIFGLENEYGLIFSPNGRVYLPMEKVLGYIFEGLI
                     PNSWPSNAFLVNGARFYQDTGCHPEYSTPECDNILDLVIHDKAGERLLEACLPAAEER
                     LREEGLSGEIYIFKNNTDSLGNTYGCHENFLMRRDVDFWKVTEQLIPFFVTRQIYSGA
                     GKVLKVSGKPQYFISQRAQHIHEKTSSSTTSSRSIINTRDEPHADAERYRRLHIIVGD
                     SNMSEFVTYLKVGTATLVLSMIEEGYVVQGMEFEDPVKAIREISRDPTLKRKIKLDDG
                     RQMTAVEVQRVYLDRAQEYLAQQVHDPILDDVWQRWAMVLDKLEEDPMQLVREIDWVT
                     KRYLIQSYIDKKGCGWDDPRVFLLDLQFHDVKRTRGLYYLMESRGLIERVVEEEAVQR
                     AMSTPPQTTRAKVRGDFIRFARAKNRSYTVDWTYLKLNGYWEETILCMDPFSAVNRRV
                     DELLSQVAGLRFYR"
     gene            complement(2068..2754)
                     /gene="prcA"
                     /locus_tag="VLL06_08210"
     CDS             complement(2068..2754)
                     /gene="prcA"
                     /locus_tag="VLL06_08210"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HSS30987.1"
                     /translation="MPMPYYVSPEQMMQDKAEYAKKGIAKGRSIIAIEYVSGILLVAE
                     NPSASLYKISEIYDNIAFAGAGKYSEFENLRKAGIRHADLKGFMYSREDVTARSLANG
                     YSQSLGTIFSQDLKPLEVEILVVQVGHNGQTNEMYRISFDGSIIDERAFAVIGGKSEA
                     VQALLKEKGFTQPPDLQAALGLCIAALDQIGNQKLSLESLEVAVLDRTRDGRRFRRLA
                     PADTKQLLAP"
     gene            complement(2757..3584)
                     /gene="prcB"
                     /locus_tag="VLL06_08215"
     CDS             complement(2757..3584)
                     /gene="prcB"
                     /locus_tag="VLL06_08215"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HSS30988.1"
                     /translation="MKLPFLPNADGPSFFDFLTQHHPTLTPTGRSGFIQAQASAEPMR
                     GAGLMSIPQATTVLAIKYQQGVVIAGDRRATEGFQIAERRIEKVFKIDEYSAMAIAGA
                     AGPCIEMAKLFQVELEHYEKLEGVQLSCEGKANKLGQMVKANLPMVFQGLVVMPLYVG
                     YDLKRGEGRIFKYDLAGGRYEESDYHAIGSGGKDARNAMREHFLKTLAEPDALKLALL
                     ALYNAADDDVGTGGPDLVRGIYPTAKIVNAAGIIDVPDQQIRGIYDGLIATRRSKEN"
     gene            complement(3613..5112)
                     /gene="dop"
                     /locus_tag="VLL06_08220"
     CDS             complement(3613..5112)
                     /gene="dop"
                     /locus_tag="VLL06_08220"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HSS30989.1"
                     /translation="MQEPTSLTTPRILGTETEFGIASRDPAAADPVSNSIAVIGHYPG
                     VTAPQAVWDYENENPLLDARGFEVEGERERPNPEYNRQLNKVLANGGRLYVDGAHPEY
                     STPECTNPREIVAFERASERILTQCLDQMLRATGRNHCVLYKNNSDGKGNSYGYHENY
                     LMSRAVPFERIVKVLAPFFVTRPIFAGAGKVGAENQTSPADYQISQRADFFECLVDLN
                     TMVRRPIVNSRDEPHAEYGKYRRLHVIVGDANMAELSTYLKVGTLAIVLDLLDAGADL
                     PQIELDDPVRSIKQVSRDLGMKETLKLTGGRSTTAVAIQRAYLKAAMGYYACRDLSQV
                     TKDILVRWEDVLDKLEQDPRLLVRELDWVAKRQMMESYMERKGCGWDDPRVRLMDLQY
                     HDVRSDKGLYFTLERSHLIERIVQDVEIARAEFTPPPGTRAYFRGRCVSKFAKSLYGV
                     SWTSVLFDVGNNQVKRVPLMDPLRGTASLTSDLLDHVDTVDALLEKLKA"
     gene            complement(5329..>5537)
                     /locus_tag="VLL06_08225"
     CDS             complement(5329..>5537)
                     /locus_tag="VLL06_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HSS30990.1"
                     /translation="GRGLRSEDLIRAIREEFKEHEDLPNTTNPDDWAKISGKKGEKIV
                     HLRTISGGPDDSRQIETVTTGHYL"
BASE COUNT         1190 a         1624 c         1442 g         1281 t
ORIGIN      
        1 cttctctttg gccaccagga cgttaaagct cagatgtttt gtctgttcac cctgcttcac
       61 ctcgacagtc agttcatagg tcccaggatc gtcctgcata tcaatcccga gcaatccaat
      121 atagccgggc ggctcctcag acctgaactc cctgaaaaat ggaatggtcc ggttcaaaaa
      181 cgtccctttt acctccgttg cccgctcttc gcccttgacc ttgaccacca gcacctgccc
      241 ctgcctgccg ctgtattgcc catcactacc cctcggcgca ggagacgtat tggaaaacaa
      301 ctcgacggga aagacgctgg acagcaagac aacggctgta atcagcatcc ggtccagcca
      361 tgagacccgc acaggcgtct gtgtgaggtt cgtcatcatc ggtaaaaccg cagccccgcc
      421 acctgcgaga gcaactcatc cacacgccga ttcacggcgc tgaatggatc catgcataga
      481 atcgtttctt cccagtaacc attgagcttg agataggtcc aatcaaccgt ataggaccga
      541 ttctttgcgc gggcaaaccg gataaaatcg ccgcgcacct tcgccctcgt agtctggggc
      601 ggggtcgaca tggctcgttg caccgcttcc tcttcgacca cccgttcgat caacccacgg
      661 gactccatca agtagtacaa cccgcgcgtc cgcttgacgt catgaaattg gagatcgagc
      721 aggaacactc gtggatcgtc ccacccgcac ccctttttat cgatatacga ctggataaga
      781 tagcgcttgg tcacccaatc gatctcgcgc acgagctgca tcgggtcctc ttcgagctta
      841 tccagcacca tggcccatcg ttgccacaca tcgtcgagga tcggatcgtg cacctgttgc
      901 gccaaatatt cctgcgctcg atccagatag acccgttgaa cttcgactgc cgtcatctga
      961 cgaccatcgt ccagcttgat cttgcgcttc aaggtgggat cgcgggaaat ctcgcgaatc
     1021 gccttgacgg gatcctcgaa ctccatcccc tgaacgacat acccttcctc aatcatggac
     1081 agcaccaacg tcgccgtacc gactttcaaa tacgtcacga attccgacat gttggaatcc
     1141 ccgacgataa tgtggagccg ccggtaccgt tccgcatccg catgcggctc gtctctggta
     1201 ttgatgatgc tacgtgagga ggtcgtcgaa gaagaggtct tctcgtgaat gtgttgtgcg
     1261 cgttgcgaga tgaagtattg tggtttaccg gataccttca ggacctttcc cgccccgcta
     1321 tagatctgcc gcgtgacaaa gaagggaatg agttgttccg tgaccttcca aaagtcaacg
     1381 tcacgccgca tgaggaagtt ttcatgacat ccataggtat tgccgagcga atccgtgttg
     1441 ttcttgaaaa tgtagatttc tcctgacaga ccttcttcgc gcaatcgttc ttcagctgcc
     1501 ggcagacagg cttcgagcag tcgctcaccg gctttatcgt ggatgacgag atcaagaatg
     1561 ttgtcgcact cgggggtaga atactcagga tggcagcctg tgtcttgata aaacctagcc
     1621 ccgtttacca gaaacgcgtt ggagggccag ctgttcggaa taagaccttc aaaaatatag
     1681 cccaacacct tctccatcgg gagatacacc cgtccgtttg gagagaagat gagaccgtac
     1741 tcattctcta aaccgaagat ccgccgtttc aggggaatgg gctgcaacat gctagtacca
     1801 acagaatagc agccagcctc gccttcttca acagaaggac acgatccgtc gatgaaggtt
     1861 ggccgtcagc cttacagaaa actattggta cgatggaccg tttgaggtca gcacgtgtgg
     1921 cacaatgaaa tataattccc gcaagctgct caaaaagccc gtccagcaag gccgcaggcg
     1981 agttgaaacc ggagacgtac cctcggaggg tacgttgagg atttcgacga gtcgagaacg
     2041 ttgctggcgg gctttttcag cggcttgcta gggagcgagg agctgtttgg tatcagccgg
     2101 cgctaaccgg cggaatcttc gtccgtcgcg agtgcggtct agaaccgcca cttccaaact
     2161 ctccagtgag agcttctggt tcccaatttg gtcgagtgcg gcaatacaga gtccgagggc
     2221 agcctggaga tccggaggct gtgtaaaccc tttctccttg agcaaggcct gcacggcctc
     2281 ggatttgcct ccgatcaccg caaaggcacg ctcgtcgatg atgctgccat caaacgatat
     2341 gcgatacatc tcattggtct ggccattgtg cccaacctgc accaccagga tttccacctc
     2401 cagcggtttc aaatcctggc tgaaaattgt gccgaggctt tgggaatagc cgttcgcgag
     2461 agagcgggcc gtcacatctt cacggctgta catgaacccc ttgagatccg cgtgtctgat
     2521 gccggccttc cgaagatttt caaactcgct gtacttaccg gctcccgcga atgcaatgtt
     2581 gtcatagatc tcggaaattt tatacaacga ggcgctgggg ttttctgcga cgagcagaat
     2641 gccactcaca tattcgatgg cgatgatgga gcggcctttg gcgatgcctt ttttggcata
     2701 ctccgccttg tcctgcatca tctgctcggg cgagacgtaa tagggcatcg gcatgtctaa
     2761 ttctccttgg agcgacgggt cgcgatcaac ccatcgtaga tgccacgaat ctgttggtcg
     2821 ggaacatcga taatgcctgc cgcgttcaca atttttgcgg tggggtatat gccccgcacg
     2881 agatcaggtc ctccggtgcc gacatcatcg tcagccgcat tatacagagc cagcagggcc
     2941 aacttcagcg cgtccggctc agccaaggtt ttgaggaaat gctcccgcat cgcattgcgt
     3001 gcatctttcc ctcctgaccc tatcgcatgg taatccgact cttcgtaccg gccacccgcg
     3061 agatcgtact tgaaaatgcg gccttcacca cgcttcagat cgtagccgac atagagcggc
     3121 atgacgacaa gcccttgaaa gaccatcggc agattagcct tgaccatctg tcccaactta
     3181 ttggccttgc cctcacagga cagttgcaca ccttctaatt tttcgtaatg ctccagctcg
     3241 acttggaaca gcttggccat ttctatgcag gggcccgccg caccggcaat cgccatcgcg
     3301 gaatactcgt cgattttgaa gactttctcg atccgccgtt cggcgatttg aaatccttcc
     3361 gtcgctcgac gatctccggc aatcaccaca ccctgttgat acttgatcgc caataccgtg
     3421 gtggcctgtg gaatgctcat caagccggcc ccacgcatcg gctcggccga agcctgggcc
     3481 tgtataaagc cactccggcc ggtcggagtc agcgtgggat ggtgttgggt caaaaagtcg
     3541 aaaaaactcg gaccgtcggc gttggggaga aatggcaatt tcatggtgtg aatcgcgagg
     3601 ggtccttgcg cattacgcct tgagcttctc cagcaacgcg tcgacggtgt cgacatgatc
     3661 gaggagatcg cttgtcagcg aagccgtccc tctgagcggg tccatcaatg gcacccgttt
     3721 gacctggttg tttccgacgt cgaagagcac cgaagtccag ctgaccccat agagcgactt
     3781 cgcaaacttg ctgacacatc gtccacggaa ataagcgcgc gtccctggag ggggggtgaa
     3841 ctccgctctc gcgatctcga cgtcctggac gattcgctcg atcagatgac tgcgttcgag
     3901 cgtaaaatag aggcccttgt cagaacggac gtcgtgatac tgaaggtcca tcagacgcac
     3961 ccgggggtca tcccacccac agccctttcg ctccatatag gattccatca tctgacgctt
     4021 tgcgacccaa tccaactcac ggaccaagag acgaggatcc tgctccaact tatccaacac
     4081 gtcttcccag cggacgagaa tatctttcgt gacttgtgac agatcgcgac aggcatagta
     4141 tcccatggcg gccttcaaat aggctcgttg aatcgccacc gccgtcgtcg aacggcctcc
     4201 tgtcagtttg agcgtctcct tcatgcccaa atcgcgagac acctgtttga tacttcggac
     4261 cggatcgtcc aactcgatct gcggcagatc cgccccggcg tcgagaagat ccagaacaat
     4321 ggccaacgtg ccgaccttga ggtacgtcga cagttccgcc atgttggcat ccccgacgat
     4381 cacgtgcaac cgccgatact tcccatattc cgcatgcggc tcatcgcgtg aattgacgat
     4441 gggacgcctg accatcgtat tgagatcgac gaggcactca aagaaatctg cacgctgcga
     4501 aatctgataa tccgcggggc tggtctgatt ctctgcgccg acttttccgg ccccggcaaa
     4561 aatggggcgc gtcacgaaga acggggccaa gaccttcaca atgcgctcga aggggaccgc
     4621 acgagacatc aaatagtttt cgtgatagcc gtagctgttc cccttgccat ccgagttgtt
     4681 cttgtacagc acacaatgat ttcgcccggt ggcgcgcagc atctgatcaa ggcactgggt
     4741 gaggattcgc tcgctagctc gttcgaaagc cacgatttct cgtgggttgg tgcactcagg
     4801 cgtggagtat tcgggatggg cgccgtcgac atagagacgg cccccattcg ccagcacctt
     4861 gttcaactgg cgattgtatt caggattggg ccgctcccgc tccccctcga cctcgaatcc
     4921 acgcgcatca agcagcggat tttcattttc atagtcccag acggcctgag gcgccgtcac
     4981 gccgggatag tgcccgatga ccgcgatgga attggagacg gggtccgcag cggctggatc
     5041 gcgcgatgca atgccgaatt ctgtttctgt tcccagtata cggggcgtcg tcagcgaggt
     5101 aggttcctgc atgggtgtga cgttttagca ggatgctgaa aaagcccgcc agcggcgttc
     5161 tcgcttcact cagaagctca acgtaccgaa gcgtacgcct cgcctcttcg cttgctgcgg
     5221 ccttgctgga tgtctttttg agcatcctgc gaatgtgtgc tcctcttgtt gtcccaaacg
     5281 tgtcgatcga tgtcgttcta ctgtgcaaat tggttttccc gcagcctgtt agagataatg
     5341 gcctgtcgtg acggtttcga tctgacgcga gtcgtccggg cctccgctga tcgtccgaag
     5401 atgcacgatc ttttcacctt tttttcctga gatcttcgcc cagtcgtcag gattggtcgt
     5461 attgggcagg tcttcgtgtt ccttgaattc ttcgcgaatt gcacggatca agtcctcgga
     5521 ccgcaggcca cggcctt
//
LOCUS       DATIPF010000329         6105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1478916, whole genome shotgun sequence.
ACCESSION   DATIPF010000329 DATIPF010000000
VERSION     DATIPF010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478916"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1048
                     /gene="ylqF"
                     /locus_tag="VLL06_08230"
     CDS             188..1048
                     /gene="ylqF"
                     /locus_tag="VLL06_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HSS30991.1"
                     /translation="MSIQWFPGHMNVARKEAAKTMEVIDVIVEVLDARIPNASCNPLI
                     EELRLARQRPSLKVLNKADLADPAVTQAWLSLYHRQPGVSAAALSCNHAGDAARVPQL
                     CQALAPHRNSSAKPLRMLIMGIPNVGKSTLMNTLLKRRIARVGDEPAVTKVQQRHKLN
                     DHMAITDSPGLLWGTITDPGVGLLLAATNAVGHSVVDDEVIAEFLAAILLVRYPARLT
                     TRYGFAVDGLTSAGLIDAIARKRGCLLTRSDGGLDRDKAARILLSDYRNGTLGRTSLE
                     TPESRSEDQH"
     gene            complement(1107..2570)
                     /locus_tag="VLL06_08235"
     CDS             complement(1107..2570)
                     /locus_tag="VLL06_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HSS30992.1"
                     /translation="MKHHHRTDLKHIARRAMIERGLLPDFSAEAMAELARIEAVPSDR
                     HSDIRHLTSLLWASIDNDDSRDLDQLTVAEPRSDRSVKILVAIADVDAFVTKDSALDA
                     HAKHNTTSVYTSGDMFPMLPEKLSTDLTSLGEGQDRLAIVVEMVIAEDGAVLSSTLFR
                     ALVRNQAKLAYNSVAAWLAGETPEPERVKAVSGLAAQLRLQSQVAQRLKTRRHQQGAL
                     SLETIEPRAVFDGEVLTALRVEQKNRAKELIEDFMIAANQATVSYLNGKGVPSFRRIL
                     RSPERWQRIMEVAARWGGHLPPEPDASALEAFLVTRRQADPIRFPDLSLAVVKLIGRG
                     EYVLDQSKDGAPEHFGLAVKGYTHSTAPNRRFPDLITQRLSKAALAGAPTPYRPDELK
                     YLADHCTEKEDDAEKVERQLRKSAAALLLESRIRQRFDAIVTGVSDKGTWVRLLEPPV
                     EGKMVTGANGLDVGDSVRVELVSTDVERGYIDFVRVR"
     gene            2948..3418
                     /locus_tag="VLL06_08240"
     CDS             2948..3418
                     /locus_tag="VLL06_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30993.1"
                     /translation="MRNNIAITYLRWIIAIPAGLLAWFGANHSIGMTFGFIHGYDVLD
                     SFWEAPDMDGMLITGTYIIVITRIVASASLVAVIIYMVPRYHKQVATVTTAVVSAAAV
                     ALLGFIWVQEFRADVALGPEGWYRYILDMLSIIIGAVFGAWMAYGSRRRRAPAS"
     gene            complement(3556..4071)
                     /locus_tag="VLL06_08245"
     CDS             complement(3556..4071)
                     /locus_tag="VLL06_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS30994.1"
                     /translation="MAGKVNSGSRRPAVEKKKATPGRRAKKPKPTSAAVLVLSGSTAL
                     RRRKSAEMLAETLKVDLFRVDVSAVVSQYIGETEKNLNDVFDKAEASGAILFFDEADI
                     LFKARGEVPDANDRYANAETAHFLQRIESHTGIVILTTNAKLPIERAFSPRTQVVVMV
                     GTTGIRRKRKQ"
     gene            4238..4552
                     /locus_tag="VLL06_08250"
     CDS             4238..4552
                     /locus_tag="VLL06_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30995.1"
                     /translation="MLIEQELAAAQAAQQNGNYGRARVCARRAVALAIEAWLARLPYP
                     PRHRDAMEHLRYIQQDGSLPLPIRQAAERLSTPVTRQLIAPFTTDPIADAQLIIAHLS
                     NH"
     gene            complement(4699..>6105)
                     /gene="zwf"
                     /locus_tag="VLL06_08255"
     CDS             complement(4699..>6105)
                     /gene="zwf"
                     /locus_tag="VLL06_08255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HSS30996.1"
                     /translation="LTRRKLLPALYELRDQGVLDTRNTLIVGAAMSELSEKEYRLWAI
                     EGLQQAGCGEEAELHAWCEECLHYQALQKEGVQDYGSLAKYIRRLERKHHMPENRVFY
                     LALPPDVVPIAMEKLDHVKLLKSRGWVRVVFEKPFGHDFHSARRLNTTLHQYIEEPQI
                     YRIDHYLGKETVQNLLAFRFANPIFESLWNRNIIENVQITVAEDLGMEHRGAYYQQAG
                     AFRDMLQNHLTQLLTVVAMEVPVSFDAASIQNERLKVLHSINPIAPQDVVFGQYTAWE
                     IVGQKIPGYREEEGVPSNSTTETYVALKAEVHNWRWKGIPFYLRTGKRFPRKVTQIAV
                     TFREAPTQVFRFLKPGSISPNQLLIMLQPNEGFSLCFSVKSPGRPFRLSDHALQFDYG
                     KAFGQLPEAYKTLLRDVMIGDQTLFVSADFIETAWRLYDPLLIGEKPLHFYTAGSWGP
                     SAADALLEKDGHRWGLGW"
BASE COUNT         1239 a         1836 c         1683 g         1347 t
ORIGIN      
        1 gaacagagcg atcgcctggt ctcggagcag agtccggaga ggtgttcgcg tgggccattg
       61 gtgcccctcg atgcacgaag agttaagcga tgtgttctat caatgggaag ctgattacga
      121 ctaagatgga cggatgaggc gatagccaaa ccgatgatag cgctgcgacc caggagtctg
      181 tccttctatg tccatccagt ggtttcccgg tcacatgaat gtggcgcgca aagaagcggc
      241 caagaccatg gaggtgatcg atgtcattgt cgaagtgctg gatgcgcgca tcccgaacgc
      301 gagctgcaat ccgctgatcg aagaattgcg cctggcccgc cagcgtccca gcctgaaagt
      361 cctcaacaag gccgatctcg ccgatcccgc cgtcactcag gcctggctca gtctctatca
      421 ccggcaaccg ggggtcagcg ccgcagccct ctcttgcaat catgcaggcg atgccgccag
      481 ggtaccccag ctctgtcagg cgctggcccc ccaccgaaac agcagcgcga aaccgctgcg
      541 catgctgatc atgggaatcc ccaacgtcgg taaatcgacc ctgatgaaca cactcctcaa
      601 acgccgcatc gctcgtgtgg gcgacgagcc agctgtgacg aaagtccagc aacgtcacaa
      661 actcaacgac cacatggcca tcaccgactc acccggactg ttgtggggaa ccatcacaga
      721 ccctggcgtg ggcctcttgc tcgccgcaac caatgccgta ggccactcag tcgtcgatga
      781 tgaagtgatc gccgaatttc tcgccgccat cctgcttgtg cgttatcctg cgaggctcac
      841 gacccggtac gggtttgctg tggacggatt gaccagcgca ggcctgatcg acgccatcgc
      901 cagaaagcgt ggctgcttgc tgaccaggag cgacggcggg ttggaccgag acaaagcggc
      961 gaggattctc ttgtcggact accgcaacgg aacactcgga cgcaccagct tggaaacgcc
     1021 ggagagccgg tcggaagatc agcactgacg acaacaaagt cacgcgtaca tcgcgaaacc
     1081 ggtaggacaa cccgacgact ctttcactat ctgactctga cgaagtcgat gtacccccgt
     1141 tcaacgtccg tgctcaccag ctccactcgc acgctgtctc cgacgtcgag gccattcgcg
     1201 cctgtcacca ttttcccctc gaccggcggc tccaggagcc ggacccacgt gcctttgtct
     1261 gacacgcccg tcacgatggc gtcgaaacgc tgcctgatcc gcgattcgag caagagggcg
     1321 gcggcggatt tgcggagctg tcgctcgact ttttctgcat cgtcttcttt ctcggtacag
     1381 tggtcggcca ggtatttcag ttcatcaggc cggtacggcg ttggtgcgcc ggccagcgcc
     1441 gccttgctta gccgttgggt gatgagatca ggaaatcgac ggttgggggc ggtggaatgg
     1501 gtgtacccct tcaccgcgag cccgaaatgt tcgggagccc catccttcga ctggtccagc
     1561 acatattcgc ctctgccgat gagcttcacg acggccagtg acaggtccgg aaatctgatg
     1621 gggtcggcct gccgacgcgt gacgagaaac gcttcgagcg cactcgcatc cggctccggg
     1681 ggtaaatgcc ctccccaacg tgcagccacc tccatgatcc gctgccagcg ttcgggagaa
     1741 cgcaggatgc ggcggaagga gggcacgccc ttcccattca gataggacac cgttgcctga
     1801 ttcgccgcga tcataaagtc ttcgatcagt tccttggcac ggttcttctg ctcgacccgg
     1861 agagcggtca acacctcacc atcaaacacg gctcgtggct caatagtttc aagactcagc
     1921 gccccctgct ggtgtcgtcg tgtcttgagt cgctgtgcca cctggctttg caggcggagt
     1981 tgcgcagcaa ggccggacac ggccttgacc cgctcaggct cgggcgtctc ccccgcgagc
     2041 catgcggcga cgctgttata ggcgagcttt gcctggttgc ggaccagggc ccgaaagagg
     2101 gtcgagctga ggacagctcc atcctccgca atcaccatct ccacgacaat cgcaagtcgg
     2161 tcttgccctt caccgagaga tgtcagatca gtcgacagtt tttccggcag catagggaac
     2221 atatcaccgg acgtatagac cgacgtcgta ttgtgtttgg catgcgcatc cagggcggaa
     2281 tccttcgtga caaacgcgtc cacgtcggca atcgccacca gaatcttcac cgaccggtca
     2341 gagcgtggtt ccgccacagt gagttggtcg aggtctcggg aatcgtcgtt atcgatcgat
     2401 gcccagagta gactcgtgag gtgacgtata tccgagtgcc ggtcgctggg aacagcctct
     2461 atgcgggcaa gctcggccat cgcctcggcg gaaaaatccg gcaacaagcc acgttcgatc
     2521 atggcgcgac gagcgatatg cttcagatct gtgcgatgat gatgtttcat ttccccctca
     2581 atcgtgaatg agacctcgat ccatctgctg aggctgtcgt atcagccctt gcctagaaaa
     2641 gacactttca gcttgctcgc cttcactttt aggatggttt cttcctgcac ttgccagaat
     2701 cgatcctgtg cgaggacaag acccgaatca gtgtgagtcc tggaatgtgc agtttcatgg
     2761 agggagtgtg cctacgaggt gcatggttct gattgcgttc tcagtttgta ccagctatat
     2821 gatagggacc taggtggtct cacactgagg ccgtccccgt cagaagaatc agtgagtcaa
     2881 gaaccccgcc ctaccgggcg gaggattctt atgcggccat ttgtaactct gatgtctggt
     2941 tcacccaatg cgtaacaaca tcgccattac gtaccttcga tggatcatcg cgattccggc
     3001 aggtctcttg gcatggttcg gcgcgaacca ttctattggc atgacattcg gctttatcca
     3061 cggctatgac gtactggatt ctttttggga agcgccggat atggacggga tgctgattac
     3121 aggcacctac attattgtga tcacgcgaat cgtggcgtct gcttcccttg tggctgtgat
     3181 tatctacatg gtcccgcgct atcacaaaca ggtggcgacc gtcacgaccg ctgtggtatc
     3241 cgccgccgct gtcgctctct tgggtttcat atgggttcaa gaattccgtg ctgatgtcgc
     3301 cctcggtccg gaaggttggt atcgatacat tctggacatg ctgagcatta tcattggggc
     3361 agtattcggt gcctggatgg cctatggaag tcggagaagg cgtgctcctg cttcgtaaat
     3421 gtttcgttcc cttggccacc tgctgcccca tctcaataat cccgccattc tcgtaggatg
     3481 ctaaggcatt ccgccggcct ctcccgagcc cttcctctcg cttcacccgg tgtgacctgt
     3541 cggactcccc cacgtctatt gtttcctctt ccgccggatc cctgtcgttc ccaccatgac
     3601 gactacctgc gtacggggag agaaggcacg ctcaatgggt agcttcgcat tggtcgtgag
     3661 gatgacgatt ccggtgtgac tctcgatacg ctggagaaaa tgagccgtct ccgcattcgc
     3721 gtatcggtcg ttggcatcgg ggacttcgcc gcgtgcctta aacaatatgt cagcctcatc
     3781 gaagaagagg atcgcgccac tggcttctgc tttatcaaat acgtcgttca gattcttttc
     3841 cgtctcgccg atgtattggc tgaccactgc cgacacgtcc accctgaaca aatctacttt
     3901 caatgtttcg gccaacatct cggcagactt ccttctgcgg agggccgtgg aaccggacag
     3961 cacgaggacc gcagcggatg tcggcttcgg cttctttgca cgacgccccg gcgtggcctt
     4021 cttcttctct actgccggcc tccggctacc actattcacc ttgcctgcca tgccttcttt
     4081 atcctcctcc cggtacgtcg aacagtaaca ggatattgca acactctgcg aacatgtgcg
     4141 gagacttgcc gatcttatcg tcaattgatc acaatggcca gcagcgtacc gttcagggat
     4201 tcagcaatct gacgggtgtg gcctcatgag accaacgatg ctgattgaac aggagctcgc
     4261 ggcagcccag gccgcgcagc agaacggcaa ctacgggaga gcgcgagtct gtgcgcggcg
     4321 cgctgtggct ctcgcgatcg aagcgtggct agcgaggctt ccctacccac caaggcaccg
     4381 ggacgcgatg gagcacctcc ggtacattca gcaggacggc tctctccctc ttcccatccg
     4441 ccaggcggcc gagcggttga gcacccctgt gactcgccaa ctgatagccc ccttcacaac
     4501 agatcccatc gccgacgcac aactcatcat cgcccatctg agcaatcact gaatgattga
     4561 atcagcaatg ctccctccaa attttctata cctctcttaa agggagcggc gaggctgccc
     4621 tttactgcgc gcatcgaacg agcacagttt catcgtgcgc gttctgcgag caaaaagggc
     4681 acctcgccgc tcccttcttc accagcccaa cccccaccga tgcccatcct tttccagcag
     4741 cgcatccgct gcacttggtc cccagctgcc ggcggtgtag aagtggagag gtttctcacc
     4801 gatcaagagc ggatcataga ggcgccacgc tgtttcgatg aaatccgcgc tgacgaagag
     4861 ggtctgatcc ccgatcatca catcgcgaag cagcgtcttg taggcttcag gaagctggcc
     4921 gaaggctttt ccatagtcga actgcagcgc atggtcgctc agtcggaacg gtcttcccgg
     4981 actcttcacg gaaaaacaga gtgagaaccc ctcgttgggc tggagcatga tcagcagctg
     5041 attgggcgaa atgctgccgg gcttgaggaa ccgaaatact tgggtgggcg cctcgcgaaa
     5101 ggtcaccgcg atctgagtca ctttgcgcgg gaagcgtttg cccgtcctca aatagaaggg
     5161 tatgcccttc catcgccagt tatgaacctc cgctttcaag gcgacatagg tttctgtggt
     5221 cgaattactc gggactccct cctcctcgcg atagcccgga atcttctggc cgacgatttc
     5281 ccaagctgtg tattgcccaa agacgacatc ctgaggagcg atcgggttaa ttgaatgtag
     5341 tacttttagc ctttcatttt gaatcgaagc ggcatcgaag gacaccggta cctccatcgc
     5401 cacgaccgtc aggagttggg tcagatggtt ctggagcatg tcgcgaaacg ccccggcctg
     5461 ctgatagtag gccccgcgat gctccatgcc gaggtcttca gccacggtaa tctgaacgtt
     5521 ctcgatgatg ttgcgattcc agagcgattc aaaaatcgga ttcgcgaagc ggaacgccag
     5581 gaggttctgc accgtctctt tgccgaggta atgatcgatg cggtagatct ggggctcctc
     5641 gatatattga tgcagcgtcg tgttcagacg tcgggctgag tgaaaatcgt ggccaaatgg
     5701 tttttcgaag acgacacgaa cccatccgcg gctcttgagc agtttcacat gatccagttt
     5761 ctccatggcg atgggcacga cgtccggtgg caatgccaag tagaagaccc ggttttccgg
     5821 catgtggtgc ttacgctcca accgtcggat atacttggcc aacgacccat agtcctgcac
     5881 tccctctttt tgcaaggcct gatagtgaag acactcctcg caccaggcat gcaattctgc
     5941 ttcctcaccg cagcccgctt gctgcagacc ctcgatggcc cagagccggt attccttctc
     6001 actcagctca gacatggcgg cgccgacgat cagcgtgttg cgagtatcca ggactccctg
     6061 atctcgcaat tcatagagcg cgggcaggag tttccggcgc gtcag
//
LOCUS       DATIPF010000330         1733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_63647, whole genome shotgun sequence.
ACCESSION   DATIPF010000330 DATIPF010000000
VERSION     DATIPF010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63647"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VLL06_08260"
     CDS             <1..483
                     /locus_tag="VLL06_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS30997.1"
                     /translation="VFKQEVARLLAKRGHTLILDRERLHAKLAEVEREIGYIMRAIKV
                     GILTVSTKAELEKAEAERGRLLSQLKARPAKAGNVTTMLPDLKERFKTLVDNLAATPH
                     DHVAKAREALKSLLGSTIALHPCADGAGRYLTAEVTGDYQGLLRLAINKNKAGGGQGS
                     "
     gene            complement(462..537)
                     /locus_tag="VLL06_08265"
     tRNA            complement(462..537)
                     /locus_tag="VLL06_08265"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(502..504),aa:Ala,seq:cgc)
     gene            complement(638..1312)
                     /gene="purN"
                     /locus_tag="VLL06_08270"
     CDS             complement(638..1312)
                     /gene="purN"
                     /locus_tag="VLL06_08270"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HSS30998.1"
                     /translation="MSTKRTDALRIAVLASGRGSNLQAVIDAIEAGMVQAKVVAVISN
                     KKEAPALERARRHGLFALFVDPKPYAGRPDSREAYDRELLDVLRQHDVELVLLAGYMK
                     IVTTALVEAFANRMMNIHPSLLPSFPGLDVQKKAIEWGCKLAGCTVHFVTEGVDEGPI
                     ILQAAVPILDDDTPDTLAARILEQEHKIYPRAVQLFAEGRLRVEGRHVFIEAGKSNGE
                     TVISPS"
     gene            complement(1302..>1733)
                     /locus_tag="VLL06_08275"
     CDS             complement(1302..>1733)
                     /locus_tag="VLL06_08275"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HSS30999.1"
                     /translation="LSMTSRLPELDQPLGDILLTPTRIYAKQILTLIQDSPIKGMAHI
                     TGGGITENLPRVFPSGVRAQVRRSAWSVPPIFQAIARLGQVERDEMYRVFNMGIGLIL
                     VVPSSAAQAVIARATALGDPACQIGAIVSSTGAEPLVEYVD"
BASE COUNT          382 a          501 c          503 g          347 t
ORIGIN      
        1 gtctttaagc aggaggttgc tcggctgctc gccaaacgtg gccacacact gatcttggat
       61 cgggaacggc tgcacgcgaa gctcgcggag gtcgagcgcg agattggtta cattatgaga
      121 gccatcaagg tgggcatcct gacggtctca accaaggcag aactggagaa agccgaggcg
      181 gaacgagggc ggcttctgag tcagctcaag gcgcgccccg ccaaagctgg caatgtgacc
      241 acgatgctgc cggatttgaa agagcggttc aaaacacttg tagacaatct ggcggcaacg
      301 ccgcacgacc atgtcgccaa ggcgcgcgaa gctctcaaga gtctgttggg aagcacaatc
      361 gcactccatc catgtgccga tggtgcgggg cgctacttga cggcggaagt gaccggggat
      421 tatcagggat tactgcggct ggccatcaac aaaaataaag ctggtggagg gcaggggagt
      481 tgaacccccg acccctacgt tgcgaacgta gtgctctccc aactgagcta gccccccact
      541 cgaccgacat gtaaccggta agacgaacgc ctcacggtcc gtcgcattat acacaaccgt
      601 ttcctgagtc tctaccgatg ttcgattcac gacgccgtta tgacgggctg atcactgtct
      661 caccgttgga ctttcccgcc tcgatgaaca cgtgacgccc ctcaaccctc aggcgccctt
      721 cggcaaagag ttggaccgct cgcgggtaga tcttgtgttc ttgctcgaga atgcgagcgg
      781 ccagtgtgtc gggcgtgtcg tcatcgagaa tcgggactgc cgcttgaaga atgatcggac
      841 cttcatcgac cccttccgtc acaaagtgca ccgtacatcc ggcaagtttg cagccccact
      901 caatcgcctt cttttgcaca tccagtccgg gaaacgaggg caagagggac ggatggatgt
      961 tcatcatgcg attggcaaag gcttccacga gggccgtcgt gacgatcttc atgtacccag
     1021 ccagtaacac caactccaca tcatgctgcc gaagcacatc gagaagctcc cgatcgtagg
     1081 cctcacgact atctggtctg cctgcatagg gttttggatc gacgaagaga gcgaacaatc
     1141 cgtggcggcg agctcgttct agcgctgggg cctctttctt gttgctgatg acggcgacga
     1201 ccttcgcttg aaccattccc gcttcgatcg cgtcgatgac tgcctggaga ttcgacccgc
     1261 gtcctgatgc caagacggcg atgcgaagtg catccgtccg tttagtcgac atactccacc
     1321 aatggctcgg cgccggtcga cgacacgatg gctccgatct ggcaagcagg atcacccaac
     1381 gccgtggcgc gtgcgatgac cgcctgggcg gcagatgacg gcaccacgag aatcaaccca
     1441 atgcccatgt tgaagacgcg atacatctcg tcacgctcca cttgtccaag ccgagcgatg
     1501 gcttggaaaa taggtggtac agaccaggca ctccgtcgca cctgcgccct cacgccggag
     1561 ggaaacactc gcggaagatt ctcggtgatg ccaccgccgg tgatatgggc catgcctttg
     1621 atgggagaat cctggatcaa tgtgaggatt tgtttcgcat aaattcttgt cggagtcagc
     1681 aggatgtccc cgagaggttg atcgagttca ggcaggcggc tggtcatgct gag
//
LOCUS       DATIPF010000331         2924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_771295, whole genome shotgun sequence.
ACCESSION   DATIPF010000331 DATIPF010000000
VERSION     DATIPF010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771295"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2042)
                     /locus_tag="VLL06_08280"
     CDS             complement(<1..2042)
                     /locus_tag="VLL06_08280"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HSS31000.1"
                     /translation="MVRTRVVVALIIILLIGGGAFLIFSPYLTGDDYLKDFFLQQLEQ
                     SIGRKIDVHRIKLVLFPRIRLELTQVAIHDRNSDEVLLSAKKIDLVLRFLPLLRKQIV
                     GKRLLIEEPTLTLRRSHNGHWNLLDQVVQGGSADQDAVQTLSRIFHIREATLVKGKLL
                     IIDEARPDGVRSMTLESVEMSLLIKAERGQADLQISAGHAGDKGLSAMSLAGTISIAQ
                     RKTLALDDPSASQTMLQFDGALEAANLSLQEVADFFGPRPVPPQVQGAVNMRSHIRAV
                     PGVAGYDVVLSDLSANLEQLKFAGQASLAGLLTPQPTFSLTLTAPPIQLSQLLTTFPA
                     EWVHPQLPVVIKDREIDGRVEIVSATVTGSVAAGPQLSLTGEFRVTQGQALIGDSRTP
                     SKDLTAVVSVEAGRLRVSKLSGLYGTIHMSESKALVSFLEAGPWLEMDITGEMAAADL
                     VQFLAKTVRSEQLSRLLAASREVEGEAIPTFRMVGPLNQPGGVTFAGGEITVQHVSLT
                     NPSLPERLTALQGRFVLSDGGTQFDQVTGQFGDVALQVSGGITGGSGSTFQDFLIRVK
                     GDAGHMVKLLPSKAISADVVDGLINGAMVLTGPTLSPHIRGEVVLTESKVTLPNLVEK
                     PIGAQATVEFEGAIPQGSGGVLLERLELIVPPIRLPVKGKIQLGERFSIDAALSTGT"
     gene            complement(2369..>2924)
                     /locus_tag="VLL06_08285"
     CDS             complement(2369..>2924)
                     /locus_tag="VLL06_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31001.1"
                     /translation="VLPEWAPSAHAHPIVMPAEEVARILGNISVKEHQIWLQKWLQGE
                     APLMPAFRPEELALLSQQIIEALAQARPDERVTFYLSQPRTSAKRVITTGGLYVHGTE
                     LHFILGNWQIVYGIPTYGMIYDRRYPMRPTAAKGFHLYFYPAEATIPQRHSLWDTLLA
                     NTDDELVIDLTKIASDPIAAIPGL"
BASE COUNT          651 a          863 c          809 g          601 t
ORIGIN      
        1 gtgccggttg aaagcgctgc gtcgatcgaa aaccgttcgc ccaattgaat cttgcctttg
       61 accggcagcc ggatcggcgg cacgatcagc tcgagccgct cgagcaggac ccctccggag
      121 ccctgtggaa tggctccctc gaattccacc gtcgcctggg cgccgatcgg tttctcgacc
      181 agattgggaa gcgtcacttt tgattcagtc aggacgacct caccccgtat gtgcggagac
      241 agggtcggac cggtcaacac catggcaccg ttgatcaagc catcgaccac gtcggctgag
      301 atggctttcg agggcagcaa tttgaccata tggcccgcat cgcctttgac tcgaatcagg
      361 aagtcctgaa aggtactgcc gctgccccct gtgattcctc cgctgacctg taatgccaca
      421 tcgccaaact gtcccgtcac ctgatcgaat tgagtcccac cgtccgacag cacaaaccgt
      481 ccttgcaacg ccgtcaatcg ctcgggcaac gacggatttg tcaaactgac atgctggacg
      541 gtaatctctc ccccggcaaa ggtcaccccg cccggctgat tgagcgggcc gaccatacga
      601 aacgtgggaa tagcctcgcc ttcaacctca cgcgaagcgg ctaacagacg cgagagctgc
      661 tccgacctga cggtcttcgc gagaaactga acgagatctg cagcggccat ctccccagta
      721 atgtccatct caagccaggg tcccgcctcc aaaaatgaga cgagcgcctt gctttcactc
      781 atatggatcg tgccgtagag cccgctcagt tttgacaccc tgagacggcc ggcttcaacc
      841 gacaccaccg cggtcaaatc cttggaaggg gtacgactgt cgccgatcag ggcttgaccc
      901 tgcgtcacgc gaaattcccc cgtcaacgat agctgcgggc ctgccgctac cgatcctgtg
      961 acagtggccg agacaatctc cactctccca tcgatctccc ggtccttgat caccaccggt
     1021 agctgcggat gcacccactc ggccgggaag gtcgtgagca actggctcaa ttggatcggg
     1081 ggtgccgtaa gggtcaatga aaacgtgggt tgaggcgtga gcagcccggc gaggctcgct
     1141 tggcctgcga acttcagttg ttcgaggttt gcggacagat cggacaacac cacatcataa
     1201 cccgcaacac caggcacagc ccgaatgtga ctgcgcatat tgacggcgcc ctgcacctgt
     1261 gggggaaccg ggcgtggccc gaagaagtcc gccacctctt gaagactcag attggccgct
     1321 tcgagtgcac cgtcgaattg aagcatcgtc tgcgaagcag aaggatcgtc gagtgccaac
     1381 gtcttccgct gggcgatgct gatcgttccg gccagggaca tggcagacag gcccttgtcg
     1441 cccgcatgac cggcagaaat ctgcaggtcc gcttgtcccc gctccgcctt gatgagcagg
     1501 gacatctcga ccgactccag cgtcatcgat cgtaccccgt ccggacgtgc ctcgtcgatg
     1561 atgagcagct tccctttcac cagcgtcgct tcacggatat ggaagatccg gctgagcgtc
     1621 tgcaccgcat cctgatcggc tgagcccccc tgaacgacct gatcgaggag attccagtgc
     1681 ccgttgtgac tgcgccggag cgttaatgtc ggttcttcga tgagcagccg cttccctact
     1741 atctgtttgc gcaacagggg gagaaaccgt agaaccaaat cgatcttctt ggccgataat
     1801 aagacttcat ctgagttccg atcgtgaatc gcaacctgcg tcagttctaa ccgaatgcgg
     1861 ggaaacagga ccagcttgat gcgatggacg tcaatcttgc gtccaatgct ctgctccagc
     1921 tgctgaagga aaaagtcttt gagataatcg tcgccggtta agtagggtga gaatatgaga
     1981 aacgcgccgc cgccgatcaa cagaatgata atcagggcaa caacaacacg agttcggacc
     2041 actcctactc cctggatcga tcaagcccgg tgggtaaatc gcatcgcgtc tgcgcgtgcg
     2101 tcatcttatc gaatcacctg catgaaatct actaagtctg tgtcttggca gggaattctc
     2161 aacttctcac atacgaggtt ctgctccacc gatccagact atgagacact gcagcggaca
     2221 aaccaagagg acatgaatga tacacagtgg ctgacctctt catgtatggc ttcaagcagg
     2281 tctggaacgc taaatggaaa agaaaatccc cgggcaaaga tgctgtcttg gcgggctcaa
     2341 cgcgagtggg tgccgaacgc ggctgtgtct agagtcctgg gatagcagcg attgggtctg
     2401 aagcgatttt tgtcagatcg atgacaagtt catcatccgt attggctaac aatgtatccc
     2461 agagactgtg ccgttggggg atcgtcgcct cggctggata gaaatataag tgaaatcctt
     2521 tggcggccgt cggtcgcatg ggatagcgcc gatcgtaaat catcccatag gtcggaatgc
     2581 catagacgat ctgccaattc cccaaaataa aatggagctc tgtgccgtgg acatacagtc
     2641 cacccgtggt aatgacgcgc ttggcggagg ttcgcggttg actgaggtaa aacgtcacgc
     2701 gctcatccgg tcgggcctgg gcgagggcct cgatgatctg ctgagacaaa agggcaagct
     2761 cctcaggacg aaacgccggc atcagcggcg cctcgccctg cagccatttc tgcaaccaaa
     2821 tctgatgttc cttgaccgag atgttgccga ggatgcgagc cacttcttcg gccggcatta
     2881 ctatcggatg tgcatgtgcg ctgggagccc actccggtag gact
//
LOCUS       DATIPF010000332         4103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_64196, whole genome shotgun sequence.
ACCESSION   DATIPF010000332 DATIPF010000000
VERSION     DATIPF010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64196"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VLL06_08290"
     CDS             complement(<1..399)
                     /locus_tag="VLL06_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSS31002.1"
                     /translation="MTTTASTKKLTGKVAMVTGASRGIGAAIATRLAQDGAAVAITYA
                     GDQSKAGEVVRGIESAGGKAVAIRADSADADAVKQAVAETVRSLGRLDVLINNHGASI
                     TTPSMNLSPNGWRAVVGINLDGVFFCSKSAA"
     gene            complement(541..1200)
                     /locus_tag="VLL06_08295"
     CDS             complement(541..1200)
                     /locus_tag="VLL06_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HSS31003.1"
                     /translation="MNALDQAWQTIVGEVVLWAPKAPVGLLVFFAFWLCSVVVRNIFS
                     RIGYRSESNSRYFLKVVGRALSLAILLFGGITALGTIGINVSALVAGLGLTGFALGFG
                     LKDILANLVSGLLILLYRPFSLNEQISVAGMEGCVTEIDLRYTRLENAGKAYLIPNST
                     MITNTIMLVSRGEKTGAGNSTATAAPLMTAAAASSINSEEPVAALDGGMEVSHHTDNS
                     R"
     gene            1678..2241
                     /locus_tag="VLL06_08300"
     CDS             1678..2241
                     /locus_tag="VLL06_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31004.1"
                     /translation="MGSNLKVIGVLSCGFLLCLGLSNAAKADGPPSAVADMNASQYER
                     EGGQAGLKGEEDKLTGDPIPSQSGRKGGQAGLKGAEDKLDGSHITIPEREGGQAGSRG
                     DQDKLKGLKGSRTIKGEVLSVEGDIYYLQEQNGKAVRLHTDKTTRKIGNIHEGISIEA
                     EVNDRGHALSIRSAESSDRRNEADRKG"
     gene            2287..2826
                     /locus_tag="VLL06_08305"
     CDS             2287..2826
                     /locus_tag="VLL06_08305"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSS31005.1"
                     /translation="MRRHGLTKMSFILVSFACMALLAEYSIAAKTPQIPTSRPQTAET
                     SPQIVDGSKVTLQYVAKVSDSTGIDYGNISEFIQGRHEIFPALEREVVGMKPGEEKSV
                     QLSPEEGFGPHDDGKKLNVPKTLLPFGAKEGDILRNDVGELATVAELSDTTAVLDYNH
                     PLAGKPLVVKVKILKVENP"
     gene            complement(2933..3547)
                     /locus_tag="VLL06_08310"
     CDS             complement(2933..3547)
                     /locus_tag="VLL06_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31006.1"
                     /translation="MDQHDHADGAVDMMTPRQKHLGPHMRWTGLRTANADDTQRADHI
                     VQALRQALAKYKDYRVAIDDGYVPMHPERKPRHYHFANKQQRFLAKVRFDPAEPTALL
                     YRKAGDGYELEGAMYTAPKGMSEDQLNERVPLSVAQWHAHVNICFTPDGSGRRMTRKE
                     FGFKGKIATESECQQAGGRFVPQAGGWMIHVYPFESTPVKIWTH"
     gene            complement(3760..>4103)
                     /locus_tag="VLL06_08315"
     CDS             complement(3760..>4103)
                     /locus_tag="VLL06_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31007.1"
                     /translation="SQEAIRTAVELILRSKGIRVLTQSESLSTPAAPYLYVKVSTYNH
                     SSDFYAYAVTVDVMQKVSLAQRPQHMMSGTTWERGAVGTTDHSNIRMVIDSVESKVKE
                     FASDFLAANPR"
BASE COUNT         1008 a         1121 c         1093 g          881 t
ORIGIN      
        1 ggctgccgac ttcgagcaga agaacacgcc gtccagattg ataccgacca ccgcgcgcca
       61 tccgttgggc gagagattca tcgacggtgt cgtgatactg gcgccgtgat tgttgatcag
      121 cacgtcgaga cggccgaggg atcgaaccgt ctcagccacg gcctgcttca ccgcgtcggc
      181 atcggcgctg tcggcgcgga tcgccaccgc ttttccgccg gccgattcaa tcccgcgcac
      241 cacttcaccg gccttcgact gatcgccggc ataggtaatc gcgacggctg cgccgtcctg
      301 cgccaaccgt gtcgcaatcg ccgccccgat cccacgcgac gcacctgtga ccatggcaac
      361 cttgcccgta agttttttcg tcgatgcagt ggtcgtcatt gcctactccg ttgttgtcct
      421 agtccatcgt tcggctatac cggaccgaat gtcgtcttga gatgaccggc catgataaga
      481 agaaattctg agctttcgta tggaggaatt gcaagcggaa ggaagggact ccgttgggtc
      541 ttacctcgaa ttatccgtgt gatggctgac ctccataccc ccgtcgagcg ccgcaaccgg
      601 ttcttcgcta ttgatcgatg acgcagcagc agccgtcatc agtggggcag cagtggccgt
      661 gctgttgccg gcccccgttt tctcccctcg tgataccagc atgatcgtat tcgtaatcat
      721 cgtcgaattc ggaatgagat aggctttccc ggcgttctcc agtcttgtat agcgtaagtc
      781 tatttcggtg acacaacctt ccattccggc cacgctgatc tgttcattca agctgaacgg
      841 acgatagaga agaatcagca atcctgagac cagattcgcc aagatgtcct tcaagccgaa
      901 tcccaaggca aaccccgtca ggcccaatcc cgcaacaagc gcggagacgt taatgcctat
      961 tgtgcccagg gctgtaatgc caccgaagag cagtattgca agagagaggg cgcgtccgac
     1021 aacttttaga aagtatctgg agttcgactc ggacctatag ccgatgcggg aaaagatatt
     1081 tctgacgacg acactgcaca gccagaaggc aaagaagacc agtaggccga caggagcctt
     1141 cggcgcccag agaaccacct cacctacgat cgtttgccat gcttgatcga gagcattcat
     1201 gcgaatctac gcctgaccct ctggagtatt ccatttttgc tacatcccac cgcaaggacc
     1261 atgcccctgc gatgaggaaa tggttcgaaa gatctgcaac aaatcgaatc cagaatcaat
     1321 agatctgtag aatcatctca agtccttggg agcaaaggaa gaatagagaa accttcgctg
     1381 gactatgtcc ccgcatgact ccacatacgc ttcctcatgc caatcatgat gtcagacaaa
     1441 ttgtccagag gaggacaact cgtcgtcatc acatgcgtat ataaaaacca gtcgcttaga
     1501 tgacgcctta ttcgctcccg ggacaagctg tgttgagagc ataaccctca cactgtccat
     1561 ccccgtttcg catctccttg aaagataacc ccaaagtaaa ctcggcttag atggtattgc
     1621 ccttgctcaa aagtacgcgt gtgagtcgtt gtgttcagcc ccaacgagga gggcccaatg
     1681 ggatccaatc tcaaagttat cggtgtccta tcgtgtggat ttctgctgtg tttgggtctg
     1741 tccaatgcgg caaaggcgga tggtccgcct tcggcggtag ccgacatgaa tgcaagtcag
     1801 tatgagagag aaggcggcca agctggtctc aaaggcgaag aagataaact gactggcgac
     1861 cccataccat cccagtcggg gagaaaaggg ggacaagccg gtctcaaggg cgctgaagac
     1921 aaactagacg gcagccacat aaccatacct gaaagggaag gcggacaagc cggttccaga
     1981 ggagatcaag ataagctgaa ggggctcaag ggcagccgga cgattaaggg tgaggtattg
     2041 agcgtcgaag gcgatattta ttatctccag gaacagaacg gtaaagccgt gcgcctacat
     2101 accgacaaaa ccaccaggaa gatcggaaat attcacgaag gaatttctat tgaggctgaa
     2161 gtgaacgata ggggtcacgc gctgtcgatt cgttcggccg aatcgagtga ccgccggaat
     2221 gaagccgatc ggaagggctg aagccttctc gaatgagaag cctcaacgtt attcaaggag
     2281 ggttgtatga gacgacatgg cctgacaaaa atgagtttca tcctggtcag tttcgcttgc
     2341 atggcgctct tggcggaata cagtattgcc gcgaagactc cacagatacc gacttcccgg
     2401 cctcaaacgg ccgagacttc gccgcagatc gtggacggat cgaaagtcac actccaatat
     2461 gtagccaaag tgtccgattc gaccgggatc gactacggca acatcagcga gtttattcag
     2521 ggtcgacacg aaatcttccc ggcgttggaa cgagaagtcg tggggatgaa acccggagag
     2581 gaaaagagcg ttcaactgag tccggaggaa ggatttggtc ctcatgatga tggaaaaaaa
     2641 ttgaacgttc ccaaaacgct tctaccgttt ggagcaaaag aaggagatat cctacgaaat
     2701 gacgtcggcg agctggcgac ggtggcggag ttgtcagaca cgaccgcggt attggattat
     2761 aaccatccgc tcgcagggaa gccgctcgtc gtaaaggtca agatcctcaa agtcgagaat
     2821 ccgtgacttc ccttccttcc caagcggaaa tgatcaaaag ggtgaacttt gatcggtacc
     2881 ttctgtgaga tgtaccgaag aaatcttcac gctgatccca gtctcgcaat tcttagtggg
     2941 tccagatctt tacgggagtc gattcaaacg ggtacacatg gatcatccac ccgccagctt
     3001 gcggcacgaa ccgtccgccg gcttgctgac attctgattc cgtggcgatc ttgcccttaa
     3061 acccgaactc tttcctggtc atgcggcgtc cgctgccatc gggcgtgaaa cagatattga
     3121 cgtgggcatg ccactgcgca acgctcaaag gcacgcgctc gttcaactgg tcctcgctca
     3181 tacctttcgg ggcggtgtac atcgccccct caagttcata accatccccg gctttccgat
     3241 acaagagtgc ggtcggttca gccggatcaa agcgaacctt ggctagaaat cgctgttgct
     3301 tgttggcgaa atgataatgc cgcggcttcc gctccggatg catgggaaca taaccatcgt
     3361 ctatcgcgac ccgatagtcc ttgtacttgg ccagggcctg ccgaagggcc tggacgatgt
     3421 gatcggcacg ctgggtatcg tctgcgttcg cagtccgtaa tcccgtccat ctcatgtggg
     3481 gaccaagatg tttttgacga ggggtcatca tgtcgacagc accatcggca tgatcatgct
     3541 gatccatggc tgcgccctct ccctgtaccg gctcatccgc ctgaaccaag ggcccaccac
     3601 ccagtagaac gacctggctc cctgataaag ccagcacaca gcccagaaac aatctaaagc
     3661 agcgcgttga atatcgaacg ctttggcaag tcatcgttgc ccccttagga cacggtggat
     3721 acaaaggact ctggaagaga gctcgcacca agcacaatgc taccgaggat tagcggcaag
     3781 aaagtcgctc gcgaattcct tgacctttga ctcaacagag tcgatcacca tccttatgtt
     3841 ggaatggtcg gtggtcccta cggcacccct ttcccatgtt gtcccagaca tcatgtgctg
     3901 aggtctttgc gccagcgaca ctttttgcat gacgtcaact gtgacagcat aggcatagaa
     3961 atccgaagag tgattgtacg tggagacctt cacgtacagg tacggcgcgg cgggcgtact
     4021 gagactttct gattgagtaa ggacccgtat acccttagaa cggaggatga gttcaacagc
     4081 ggtgcggatg gcctcctgag aca
//
LOCUS       DATIPF010000333         5372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_859822, whole genome shotgun sequence.
ACCESSION   DATIPF010000333 DATIPF010000000
VERSION     DATIPF010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859822"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VLL06_08320"
     CDS             complement(<1..488)
                     /locus_tag="VLL06_08320"
                     /inference="COORDINATES: protein motif:HMM:NF020257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase RsbU N-terminal domain-containing
                     protein"
                     /protein_id="HSS31008.1"
                     /translation="METLEDQYTAALRDSIAGMGESSLQQAYELGRKALAEGLGVLDM
                     AAMYHKALAAGLPRHLTPEETALMLQAGASFFAESLSPFEMTHRGSREANVGLRQLNQ
                     TLEHEIQRIAHALHDEAGQLLASVYLAVEEVARNLPPAREPLLKVKGLLDLIEGQLRR
                     LS"
     gene            complement(508..1107)
                     /locus_tag="VLL06_08325"
     CDS             complement(508..1107)
                     /locus_tag="VLL06_08325"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HSS31009.1"
                     /translation="MTTQVIEWGVASLAMPGQSQSGDRYVVHPCPNGIMVAAIDGVGH
                     GQEAARAADLAATILAKHVQESMIGIINCCHAQLRETRGVVLSLAVINAVEGDLTWMG
                     VGNVEGVVVRAAASANPRQESLLLRGGVLGHQLPSLYASIIPIARGDTLILVTDGIHS
                     NFADGLNVSDRPQLIADRILAEYSKGSDDALVLVARYLN"
     gene            complement(1104..1505)
                     /locus_tag="VLL06_08330"
     CDS             complement(1104..1505)
                     /locus_tag="VLL06_08330"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS31010.1"
                     /translation="MASETVVPITTDTDVVAARQKGRELAAELGFVSTDSTLLATAIS
                     ELARNIVRYAKHGEIVIAPIQSGDRVGITVVARDKGPGIASILLAMQDGYSTSGGLGL
                     GLPGVKRLMDEFHLASDANVGTTVTIKKWKR"
     gene            complement(1498..1869)
                     /locus_tag="VLL06_08335"
     CDS             complement(1498..1869)
                     /locus_tag="VLL06_08335"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSS31011.1"
                     /translation="MEVPILKQGLYLIASIQAALSDVDLLHLRDALAEKVGKYRARGV
                     IVDVTVLDVMDSFASRTLRDLAHMTRLRGAETVIVGIQPEVAFAMVQLGLTLEGVDTA
                     LDLEEGLAFLDKKTKRRTSRG"
     gene            complement(1885..2820)
                     /locus_tag="VLL06_08340"
     CDS             complement(1885..2820)
                     /locus_tag="VLL06_08340"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSS31012.1"
                     /translation="MGEQKMKKNENRGSGVSPAREMPPPDDESTVSLLRELVNHLRKN
                     RTQLREEWARRITAAKLLGAMTSEEMFAEATSVYDNYVEVLETGAVEALQAYARDLSE
                     RIIPRGVETDEVVGIVLLLRDVLARSLFAKYQTDFQLLNRILDAYEPAANRIANTVAV
                     GFVQERERIIRQQQEAIRELSTPVLQVRERLLILPIIGVIDSQRARQLTEQLLRGIRT
                     NRAKVVVIDITGVAAMDATVANHLVQTVEASRLLGATVIVTGLSPEIAQTLVTIGVDL
                     GKMNTVGDLQGGIEEAERLLGYKVAPLIEVSQQPI"
     gene            complement(3877..4233)
                     /locus_tag="VLL06_08345"
     CDS             complement(3877..4233)
                     /locus_tag="VLL06_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31013.1"
                     /translation="MEYEPTNIISRIQWCRLQQQLYSITPDERAGWRAEEAGLVDALG
                     CRDRLAFMRKEHRSHFIRYQCGLEDGRALLRLSTPMPSGMTRTEGSQAPLTTPAWVGR
                     RPSQAPSRVHVRESRR"
     gene            complement(4476..5231)
                     /gene="otsB"
                     /locus_tag="VLL06_08350"
     CDS             complement(4476..5231)
                     /gene="otsB"
                     /locus_tag="VLL06_08350"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HSS31014.1"
                     /translation="MSRLILFLDFDGTLTPIVSRPSQAKLSRSVRETLRKLAHRMPVV
                     VISGRAPKDLRRRVGLKEVCYVGHHGLSYLEAGRDVRWLTTPPRRTLVRRWVQAMRIA
                     AEGIEGAIVEDKGMTVALHDRLVSPKQRSRLRRRARRMLAPWIAQGSVSLLRGRRVVE
                     ARSSSAGNKGTAVSKLLRRPWARHRVPVYFGDDRTDFDAFSVVHGRGLAIRIGGRRGT
                     AGEDARIPHPHTLHSLLDWLAARKSGGETRSAS"
BASE COUNT         1141 a         1621 c         1434 g         1176 t
ORIGIN      
        1 tgagagagtc gccttaactg tccttcgatg aggtcgagca ggcccttcac ttttaggaga
       61 ggctcccgtg cgggcggcag gtttcttgcc acctcctcca cagcgagata gaccgaggcg
      121 agaagttgcc ccgcctcatc atggagggca tgggcgatgc gttgtatttc atgctccaat
      181 gtctgattca gctgacgcag cccgacgttg gcttcccggg acccacggtg agtcatttca
      241 aatggcgaca ggctctccgc aaaaaaactg gcccctgcct ggagcatcag cgcagtctct
      301 tcaggcgtca agtggcgagg caaacctgct gccaatgcct tatgatacat ggctgccatg
      361 tcgagcacac ccagcccttc ggccagcgct ttgcgtccca gctcatacgc ctgttgcaac
      421 gaggattctc ccattcctgc gatcgaatct ctcaacgcag ccgtatattg atcctctagt
      481 gtttccatgg tctcccgcat cgtgaactta attcaggtag cgtgcaacaa gtacgagcgc
      541 gtcgtccgag cccttgctgt attcggcaag gattcgatcg gcgatcagct gcggccgatc
      601 gctcacgttc agcccatcgg caaagttgct gtggatgcca tccgttacga gaatcaacgt
      661 atctccccgc gcaatcggaa taatggatgc atacagtgac ggaagttggt gaccgagcac
      721 gccgccgcgg agcagcaaag actcctgacg agggttcgca gacgccgcag cacgtacgac
      781 cacgccttcg acgtttccga cgcccatcca ggtcaagtct ccctccactg cgttaatcac
      841 cgcgagactc aagacgacgc cacgagtctc gcgtagctgg gcatgacagc aattgatgat
      901 tccgatcata gattcctgga catgtttggc caggatagtc gccgcgaggt cggcagccct
      961 ggctgcctcc tgcccatgcc ctactccgtc gatcgccgcc accatgatcc cgttcggaca
     1021 gggatgcacg acgtaccggt cgcccgattg ggactgccct ggcattgcca aactggcgac
     1081 tccccattcg attacctggg tcgtcatcgc ttccatttct tgatcgtgac agtggttcca
     1141 acattcgcat ctgaggcaag atgaaattcg tccatgaggc gtttgacccc tggcaagccc
     1201 aggcccaacc ctcctgacgt ggagtagcca tcctgcatcg ccaacaaaat actggcaatt
     1261 cccggcccct tgtctcgagc caccaccgta atgcccaccc ggtcaccaga ctgtatcggc
     1321 gcaataacaa tttcgccgtg cttggcatag cggacgatat tccgtgctaa ttccgaaatc
     1381 gctgtggcaa ggagcgtcga atccgtggag acaaatccta gctcagccgc taactcacgt
     1441 cccttctggc gagcagccac gacatcggtg tccgttgtaa ttgggacgac cgtttcgcta
     1501 gccacgggac gtgcgcctct tcgttttttt gtccaaaaat gcgagcccct cttccaaatc
     1561 cagagctgtg tccactccct ccagcgtcaa tccgagctga accatcgcaa aggcaacctc
     1621 aggctggatc cctacaatca ccgtttccgc cccacgcaac ctcgtcatgt gggccaggtc
     1681 gcgcagggta cgcgaggcaa aggaatccat cacatcgagt accgtgacgt ccacgatgac
     1741 tccccgagca cgatacttac cgactttctc agctaatgcg tcccgcagat gcaacagatc
     1801 gacgtcactg agtgcggctt ggatggaggc gatgaggtat agcccttgct tgagaatagg
     1861 aacttccacg gtcgcagact ccctttatat aggttgttga gagacctcga tgagaggagc
     1921 taccttgtag cccagaagcc gttcagcctc ctcaatgccg ccttggaggt ctccgaccgt
     1981 attcatcttg cccaggtcta cgccgatggt gaccaaggtc tgggcaatct ccggcgatag
     2041 accggtcacg atgacggtcg cccccaatag tcgcgacgcc tccacagttt gcacgaggtg
     2101 atttgccacg gtggcgtcca tggccgcgac accagtgatg tcaatgacga cgaccttggc
     2161 acggttggtt cgaatgcccc gcagcagttg ttcggtcaac tgccgggcac gctgggaatc
     2221 gatcacaccg atgatcggca ggatgagcag gcgttcgcgg acctgaagca cgggcgtgga
     2281 gagttcccgg atcgcttcct gttgttggcg aataatgcgt tcgcgttctt ggacaaagcc
     2341 cacggccacc gtatttgcaa tacggttcgc cgcaggctcg tacgcgtcga ggatgcggtt
     2401 gagcaactgg aagtccgtct ggtattttgc gaagagcgaa cgtgcaagca catcacgcag
     2461 cagcagcacg atacccacaa cctcatcggt ttccacccct ctcggaataa tgcgttccga
     2521 gagatcacgg gcataggctt gcaatgcttc gacggcgccg gtttccaaca cctccacata
     2581 gttatcgtag acggatgtgg cttcggcaaa catttcctca ctcgtcatcg ctccaagcaa
     2641 cttggccgcc gtaatgcgcc gcgcccactc ttcacggagc tgcgttcgat ttttccgaag
     2701 gtggttgacc agctcgcgca gcaatgaaac ggtggactca tcatcgggag ggggcatttc
     2761 cctcgcaggc gagacaccac tgcccctgtt ttcgtttttc ttcattttct gttcacccat
     2821 gtgtgcctcc cgcatccata atgatattcc cctccttcat cctcgtgatg gcctggtcca
     2881 agcaccgtga ctcataccat tgtcggtcgg atattcagtc gcccgcctcc cccctgtccc
     2941 aatgggccgt catgttgaaa acattcctcc attttttccc atgaccgagg ggcccaggaa
     3001 atgttgggag tgaacagaac gctcacaacc ggcttcatct gtaatattta gaatagcttt
     3061 tggtcaatcg gtcgtcaaag tagggcatag tcctgagggg agcaataggg taatcacctg
     3121 aaggcaggcc tggtaactac ctgaggagga gggaagtctg gctctcttgc gcggtaccgt
     3181 ggtgggactg tgcgaggggt accctctcca ttagtgttcc gcggtcttgg gacaatctgt
     3241 caacagcgac tcgatcaacc agaagtttcc tggtttcctg agttctagtg cgatggtcca
     3301 cgtcgtaaag tgcattcccg atccaatcgg atctgagtgt acgacacgcg cggtggtcgt
     3361 gcgattgccg tatagggcat ccagctgcag ttctgcgccg cacggataag gacggagagc
     3421 gtggatcacg cagccgtgac gactcacctc aatggtcctt gcattcccgc cgtgcacaaa
     3481 aggagcatcc acctccttca cgaagagggg cattgactga ggaatccgtt ggctacgtcg
     3541 acgttcttgt ttgttactag gatgtatcat gcttcgatcg tcctcctctt gggccatgag
     3601 cgtctggcct ccgatctagg agcaaacttg accatgtcat ttctctccga cattgcaatc
     3661 gctccaagga tgtccctctt cgagacgcga ggaccatccc ggcgacggca gaccggactc
     3721 gtttcagtga cgggggtacg cgccgattta tgtgaatgac gtcgtcgtgg gttgcgccac
     3781 agttcaaaca ccgaaacccg tgacagccgg acagggatcc ttctcgatga tccagcagat
     3841 gttcatggac caacaatcca tcacagcgtg gacagatcat cgtcgagact ccctcacatg
     3901 cactcgcgaa ggagcctgag agggacgtct gcctacccag gccggagtcg tcagcggggc
     3961 ctggcttcct tccgtacgcg tcatcccgct gggcatcgga gtactgaggc ggaggagggc
     4021 tcgtccgtcc tcgagaccgc attgataccg tataaagtgt gacctgtgtt cttttctcat
     4081 gaaggcgagc cgatctctgc aacccaacgc atccacaagg ccggcttctt ctgcgcgcca
     4141 acctgcgcgt tcatccggtg tgattgaata gagttgttgc tggagcctgc accattggat
     4201 gcgtgagatg atgttcgtcg gttcgtactc catggttccc tcctgtgcaa gatgtgatag
     4261 tgcggcaaca cacaccacgg tactggagac agggggaacg cactatcagg taaatcccag
     4321 catttgagga gaataaaaac ctgcaagcgg gcgcaggttt gtactgccct gcttacatgt
     4381 ggacaggtct catgtgccag aagggctcac tgaaaacagt gatgaaacca cgcgaaacct
     4441 tgctggcagt ccggcaggag aatctgacag cccgatcaag atgcggaccg ggtctctcca
     4501 cctgattttc ttgctgccag ccaatccaga agtgagtgga gtgtatgggg gtgaggtatc
     4561 cgagcatctt caccggcggt tccacgccgc ccaccgattc gaatcgcaag tcctcgtcca
     4621 tgtaccacgg agaatgcgtc gaaatccgta cgatcgtccc caaaatagac cggcacacga
     4681 tgacgggccc atggtcgacg aagcaacttt gatactgccg tgcctttgtt ccctgcactg
     4741 gaggatctcg cttctaccac acgacgtccc cgcagaagac tgacggaccc ttgagcgatc
     4801 catggggcaa gcatacgccg ggcgcgccgc ctcaatcggg accgctgttt cggactgacc
     4861 aaccgatcat gcagcgcgac ggtcatgccc ttgtcctcga caatggcacc ctcgattccc
     4921 tcagccgcga tccgcatcgc ttgaacccat cgacgaacca atgttctacg cggcggcgta
     4981 gtcaaccacc tcacgtccct gcctgcttcg aggtaggaca atccatgatg tcccacgtaa
     5041 cagacctcct tcaatccaac ccgccgtcga agatctttcg gggctcggcc gctgatgacg
     5101 acaactggca tccggtgagc aagttttcgc agcgtctctc tcaccgaccg gctcagcttg
     5161 gcctggcttg gccgtgagac gatgggtgtc aacgtgccgt cgaaatccag aaacaggatc
     5221 aggcgactca tcgatatcgc cgcaagtctg gcccgaagcg acgccatggc tttatcccga
     5281 cacgctctgc aagtgattga catcaatgtc gacgtcgcgt aaacaactct cggcccagtg
     5341 ataaatatca tgcgtgctca gatgcagccg ca
//
LOCUS       DATIPF010000334         8496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_332161, whole genome shotgun sequence.
ACCESSION   DATIPF010000334 DATIPF010000000
VERSION     DATIPF010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332161"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(716..1054)
                     /locus_tag="VLL06_08355"
     CDS             complement(716..1054)
                     /locus_tag="VLL06_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSS31015.1"
                     /translation="MFSLQISILDIKPDQLHDGEHWFVSCISAKPFCLYGKVSVSETA
                     KMRDGLSKREGEVLSWLQQGMSNKEIAIALEVSPRTVQKHLQRIYLHLDVPTRAEAIV
                     RMRRGLRDIQ"
     gene            complement(1348..2796)
                     /locus_tag="VLL06_08360"
     CDS             complement(1348..2796)
                     /locus_tag="VLL06_08360"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HSS31016.1"
                     /translation="MKHTQTDKTATSTNATRVERDTMGELAVPAAAYYGVQTARAIEN
                     FPISSLRFPRAMIRAMGMIKRAAATVNQSLGLLDKKSADAINQAATEVVEGKLDAEFP
                     VDIFQTGSGTSTNMNTNEVISNRATELLGGARGSKLVHPNDHVNLGQSSNDVIPTAIH
                     IAAGEMMQQQLIPALTCLQKSLARKAKEFDRVVKIGRTHLQDATPVRLGQEFGGYARQ
                     IELGIQRVKRSQDVLSEVALGGTAVGTGLNCHPKFSAKVLAIISKETGCTFLEAKNHF
                     EAQSTQDSLVEASGELKTIAVSLMKIANDIRWLGSGPRCGLGEINLPETQPGSSIMPG
                     KVNPVIAESVTMVCAQVIGNDVTVTVGGQAANFELIVMLPVMAYNVLQSIELLSTAAT
                     NFAVKCIDGIKANEERCRSLIEESLAMCTALAPEIGYEAAAKLAKDAYKSGKTVRQMA
                     KEQKVLPEKRLTELLDPWRMTEAGGPVGSAGG"
     gene            2886..3122
                     /locus_tag="VLL06_08365"
     CDS             2886..3122
                     /locus_tag="VLL06_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31017.1"
                     /translation="MAHVTVEAYAGYKGEETPRAFILEGTRLRVSEIVARWYTETHSY
                     FRVCASDQHRYVLRYDLDHMRWELVMCETTNKGV"
     gene            complement(3187..3636)
                     /locus_tag="VLL06_08370"
     CDS             complement(3187..3636)
                     /locus_tag="VLL06_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSS31018.1"
                     /translation="MTLVRWDPFRELEDMSDRLNRMFSRPALPRTNGKETMVVADWVP
                     SVDVSETDGGYQIKAEIPDVKKEDVKVTLEDGVLTIQGERRQEKEETGKKYHRVERSY
                     GSFVRSFTLPDLVDEGKVKAEFKDGVLNLQLPKSEKAKPKAIEVKVG"
     gene            complement(3786..4919)
                     /locus_tag="VLL06_08375"
     CDS             complement(3786..4919)
                     /locus_tag="VLL06_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HSS31019.1"
                     /translation="MPHDFMPGLAGVPAAKSAISDVDGQRGVLEYRGIRVEELCLRSS
                     YLETAYLLLFGHLPTEVQLAQWHKDIVHHRRIKFKIVDLLKCLPEHGHPMDALQAAVA
                     ALGMFYPGRNVQDIENNYWSAVRLVAKLPTIVAAWARLRHGNDYIPPRDDLGFSENFL
                     YMLTETAPLPLWGDIFDDCLILHAEHTMNASTFTGMVTASTLADPYTVVASSIGALKG
                     PLHGGANEEVVQMLKEIGEPKRVRSYLEEQVRAQQKLMGFGHRVYKVKDPRATILQGL
                     CERLFKECGSSPLYEVALDVERVAEELLKGKRIYPNVDFYSGIVYDKMGIETDLFTPL
                     FAMARVSGWLAHWLEQLRENKLFRPDQIYSGEHNRPYVPIDQR"
     gene            5291..5680
                     /locus_tag="VLL06_08380"
     CDS             5291..5680
                     /locus_tag="VLL06_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31020.1"
                     /translation="MTTRLPLLLMLFVLILMGTGSVIQADGEILGFAVVSEVPKDKAR
                     ISAKVSVHDAVSDMKLLASETILNNLIWKKLEICHAMKLEGHKVAEGFQVVTVHVIDA
                     SMLPMSLQSFAGDCLIKKALEIAPMVD"
     gene            complement(5691..6068)
                     /locus_tag="VLL06_08385"
     CDS             complement(5691..6068)
                     /locus_tag="VLL06_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31021.1"
                     /translation="MPTPTFGTSSTGKFSCATDTQHTLRDLRTKRKGQPVFVVGHVLA
                     RKGQEGTFDVFNDRLAIVKFHDGGVIGYDPLELLLPTHIDDKGTAYFEIRPCRQCEQL
                     FSLTTEECEAPDEPPTCPECRLA"
     gene            6164..6514
                     /gene="arsC"
                     /locus_tag="VLL06_08390"
     CDS             6164..6514
                     /gene="arsC"
                     /locus_tag="VLL06_08390"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248978.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HSS31022.1"
                     /translation="MAEVTIYQKPTCSTCRQAVQLLKESGKPFTTVNYYERPFSKAQL
                     KSLLKKAGLSPKEILRTKEDIYKELGLAKKDLSDDEWLEVLIAHPDLIQRPIVEKGEK
                     VILARPAESVKELL"
     gene            complement(6524..7831)
                     /locus_tag="VLL06_08395"
     CDS             complement(6524..7831)
                     /locus_tag="VLL06_08395"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HSS31023.1"
                     /translation="MPHLIGKSLLAVLFPGGLIILLAIGFLRPQGLPMWLQQPVAALP
                     YIVLMFGLIFGWYFSSSRMILSLLVLALADRALVFFPVTDADQAAPGQTMVAVTAFLV
                     PLNLLAFSILKEDSLSTLRGVMRVLLVLVQPFLLLWLCLPDQHDLASSFTREYIPPLY
                     TDWTPIPQPALVAFALALLLHFIRFALHRDPLEGGAIWVLFAVFAAYHTSRYGWQSTN
                     FFMAAGLILFVTLLQSFYQRTYRDELTGIPGRLAYEEAIRQLGKQFSIAVISIDQLTH
                     YANTHGKPVSEQILKLVAPRIQAACVNAQIFRTTGEDLTVLFPGKCATETLSTLEGVR
                     KAIDVISLFLRGQDRVWETQGGVKKAGSRDRELPVTLSIGVGEKLNDNATLSLVIKSA
                     YHALYEAKGIGGNVVKRGPVASAPPKRSHAGSGKIVASGEYET"
     gene            complement(8052..>8496)
                     /locus_tag="VLL06_08400"
     CDS             complement(8052..>8496)
                     /locus_tag="VLL06_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31024.1"
                     /translation="RGLRGVHSSEIDRHGEQLLAIITSALALPPSAWPKVPSERKPDP
                     DSTGIVELLQAVLKARAAEKGIAPTMLATSADLQTLVEAKQHRTALDIPILHGWRRQL
                     AGDLLLEVLGGGVTISVDPTSGVLRMAQDVPPMHQANKPSLIPLA"
BASE COUNT         1935 a         2187 c         2378 g         1996 t
ORIGIN      
        1 ggctcatggc ccgaagccgt acactggcat cgggagagtt gaggtctttg gttatcgagt
       61 cgggtatgag ggggaccgag ttagcgttgg gtatatcagc aggctgggat tgaatcccgt
      121 ctccagagcc tctgatgttc acacccggga cgtccccggt gcctgacgca gagccagtcc
      181 cattagctgg ggcaaaaggc gtagggttgg tcgccggagg agctgggtcg ttacgtggcg
      241 aagaactctt ctgaaccagg ggctgtgctg ccacgcccgc acgcggccca aggtttgttg
      301 cgttatcagg ggctggtcca cacgcggcca gtcccgcgag gaacgcgacg ctcaggagca
      361 gcgccaactg cgtggctagc agatgctggg acggttgcag aggaggcata ttttcaggct
      421 tttcagataa cgcgggcgat gagggcataa aaaagcgttg ttcccgtagg attatttttc
      481 gccaaaaata tcttttggtt gtctggtatc tgcctacaag ttgtgggatc tggtctgatt
      541 tgcatgcagc cagcctgagt cttgaagcga cacgcctctc acttcgctga gctcatttca
      601 tgcggtccca agctgggcaa tacttctagc tctttgactc atgataaaca aggtgcaaaa
      661 tggcgtacaa ataaacacgt aatccacttc ttaagcccta tccatacaac caccctcatt
      721 gaatgtctct gagacctcgc cgcatccgaa caattgcctc ggctcgtgta gggacatcta
      781 gatgcaaata gatacgctgc agatgctttt gaacggtgcg tggactgacc tcgagcgcga
      841 tcgcaatttc cttgttcgac attccctgct gaagccagga caggacttcg ccttctcttt
      901 ttgaaagacc atcacgcatc ttcgctgttt ccgacacact cactttgccg tacaggcaaa
      961 atggcttcgc cgaaatgcaa gatacaaacc aatgctcccc atcgtgcaat tgatcgggtt
     1021 tgatatcgag tattgagatt tgtaaggaaa acatgactcc actggaagga tagagatatg
     1081 agagaacgag gaatgatgga atatggtgaa tcaattcaga ttcatgctga atgcaaaatg
     1141 atcctcctat gtgccggaca caaacgatag gtctagcggc gaaattatta atcccatcac
     1201 ggaataactg tagccgagtg gaggactggc catacgcttc tggaaagaga tgcggacagt
     1261 atgagcaacc tatcatccgt ctgctcgtcg ggagcctgta ccacttcaca ctgcatgcgt
     1321 cgaatgagtg cagtagcctc gctgtctcta acccccagca cttccaacag gcccacccgc
     1381 ttcggtcatg cgccagggat caagcaattc agtgagccgc ttttctggta ggactttttg
     1441 ttccttggcc atctggcgca cggtcttgcc tgacttgtaa gcatctttgg cgagtttcgc
     1501 ggcagcctcg tacccgatct ctggagcgag ggccgtgcac atggcgagac tctcttcgat
     1561 caaactcctg catcgctcct cgttggcttt gatgccatcg atacacttga ccgcgaaatt
     1621 ggtcgccgcg gttgagagga gttcaatgga ttgtaatacg ttataggcca tgaccggcag
     1681 catgacgatc agttcaaaat tggcggcctg ccctcctact gtgacggtga catcgtttcc
     1741 aatcacttgc gcacagacca tcgtcaccga ttcggcaatg accggattta cctttcccgg
     1801 catgatagac gaacctggct gtgtctctgg cagattgatc tccccgagcc cacagcgagg
     1861 gccggagccg agccagcgaa tgtcattggc gatcttcatc aggctcacgg cgatggtctt
     1921 caactccccg ctcgcttcga ccaacgaatc ctgagtcgac tgcgcttcaa agtgattttt
     1981 cgcttctaga aatgtgcagc cggtctcttt tgaaataatg gccagcacct tggctgagaa
     2041 cttcggatgg caattgagcc ccgtgccgac tgcggtaccg ccgagcgcga cttcactcag
     2101 cacgtcttgt gatcgcttga cgcgttgaat gcctaattca atctgcctcg catacccacc
     2161 gaattcctgt cccaatcgca ccggcgttgc atcctgcaaa tgggtgcggc cgatcttgac
     2221 gaccctatcg aactctttgg ctttgcgggc gagcgatttc tgcaggcagg taagtgcagg
     2281 aatgagctgc tgctgcatca tttctcctgc ggcgatgtga atcgcagtgg gaatcacgtc
     2341 gttgctagat tggcccagat tgacgtggtc gtttggatgc accagcttgc tgccccgcgc
     2401 tccgcctagc aactcggtgg cccgattgga aattacctcg tttgtgttca tgttcgtcga
     2461 ggtcccggag ccggtttgaa aaatgtccac ggggaattcc gcgtcgagct tgccctcaac
     2521 cacttctgtc gcggcctggt tgatcgcatc cgctgatttc ttatcgagga ggcccagcga
     2581 ttgattgacc gttgcggcag cacgcttgat catacccatt gcgcggatca tggcacgggg
     2641 aaaccgcaaa gagctgatcg gaaagttctc gatggcgcgg gcggtctgca caccataata
     2701 ggccgcagcc ggtacggcaa gctcgcccat agtgtcccgt tcgactctgg tggcattcgt
     2761 tgatgtcgca gttttgtcgg tctgtgtgtg cttcatgcgt tccactccat ggtaagggtt
     2821 ctcactcgtt gcgggcgtca tgataaactc acgccgcttg cttgcacaag ggtatggaga
     2881 cgaacatggc gcacgtcacg gtcgaagcct acgctgggta taagggtgag gagacccctc
     2941 gggcatttat cttggaggga acccgattac gcgtgagtga gatcgtggcg cggtggtaca
     3001 cggagacgca tagttacttt cgggtgtgtg ccagcgacca acatcggtat gtgttgcggt
     3061 atgatctgga tcatatgaga tgggaactcg tgatgtgcga aaccacgaac aaaggggtgt
     3121 gagaaccaac atgaagaggc ccgccggagc gggcctctgt gcatgcttga tgtgcaagtt
     3181 agtgaattaa ccgactttga cttcgatcgc tttcggtttt gccttttcgg acttgggcag
     3241 ctgaagattc agcacaccat ccttgaattc ggccttcacc ttcccctcgt ctacgagatc
     3301 aggcaaggtg aagctccgga caaagctgcc ataggaacgc tctacccgat ggtacttttt
     3361 tccggtctct tccttttctt gcctgcgctc accttggatc gtgaggacgc catcttctag
     3421 cgtgaccttc acatcttctt tcttgacgtc agggatctcc gctttgatct ggtatccccc
     3481 gtctgtttcg ctgacatcca cagacggtac ccagtcggcg acgaccattg tctccttgcc
     3541 attggtccga ggcagcgcgg gacggctaaa catccgattc aaccgatccg acatgtcttc
     3601 gagttcacgg aacggatccc accgtacgag tgtcatgttc aacctccttc tcattcgtgt
     3661 tggctgtttc agaacgtcta ctggaggcgc gcgccgattc gtctatctgc ggtgatagat
     3721 aactcggtct gtcatggagt caagggggct gaccgctcat ggaagtcgcg gtgccagaag
     3781 aggaattagc gttgatcgat gggcacgtac ggccgattgt gctcaccgga ataaatttga
     3841 tctggacgaa atagtttgtt ctctcgaagt tgttccagcc aatgggctag ccacccggat
     3901 acccgtgcca ttgcgaacaa gggtgtgaac aggtctgtct cgatccccat cttgtcgtag
     3961 acgatcccgg agtagaaatc gacgttggga taaatcctct tccccttcag caactcctcc
     4021 gcgactcgct cgacatctag cgccacctcg tacaacggtg aactgccaca ctccttaaag
     4081 agtcgctcac agagcccttg taggattgtc gcacggggat ctttgacctt gtacacccga
     4141 tgaccgaagc ccatcagttt ttgttgtgcg cgaacctgtt cttccagata cgatcggacc
     4201 cgcttcggct caccaatttc cttcagcatc tgtacgactt cttcgttagc cccgccatgc
     4261 agtggccctt tgagggcgcc gattgaggac gcaacgaccg tatagggatc ggccagtgtt
     4321 gaggctgtga ccataccggt gaaggttgag gcattcatcg tatgttcggc atgcaggatc
     4381 aagcagtcgt cgaaaatatc gccccacaag ggaagcggcg ctgtctcagt cagcatgtag
     4441 aggaagtttt cagaaaatcc gagatcgtcg cgcgggggaa tgtagtcgtt tccatgccgc
     4501 agacgtgccc aggccgcgac gatcgtgggt aatttggcaa caagacggac ggcagaccaa
     4561 taattatttt cgatatcctg gacattgcgt cctggataaa acatacccaa cgcggcaacc
     4621 gcggcttgca acgcgtccat gggatgccca tgctccggaa ggcatttcag taaatcgacg
     4681 atcttgaact tgatgcgacg gtgatggacg atgtccttgt gccattgtgc gagctgaact
     4741 tctgtgggaa ggtgtccaaa aagtagcaga taggcggttt cgagatagga cgatcgcagg
     4801 caaagttctt cgacgcgaat gccgcgatat tccaacacgc cgcgttgacc gtccacatcg
     4861 ctaatggcag atttggctgc tgggacgcca gcgagccccg gcatgaaatc gtgtggcata
     4921 atcgtgctcc ttcacctcac gtagcagcct acgtgaagag aggagccagt ctatcatatg
     4981 ttcggagagc gaggatcttg aattggctca tatccgttgg catactccag ttgccggccg
     5041 ttcatgtcaa gattacggct catctcctca gtgggcgtga cctttattga catcgtgttc
     5101 atatcttgga ggtcatgatg ccttggccga tagcggtgac aacaggaacg gcaatgtcga
     5161 tagttcgatc ggtacgagtg tcgtgagcga cagggcaacc tcttccgcgg accagctgag
     5221 gtctcatacg gagcacggcc agggtactat ccgttcaccc gaggaattgg ctatgcagag
     5281 gaggtaaacg atgacgactc gtctgcccct tctgctgatg ctattcgttc tcatcctgat
     5341 ggggacaggt tccgtgatcc aggccgatgg cgagatcctt ggttttgccg tcgtgagcga
     5401 ggtgccaaaa gataaggcgc ggatatcggc gaaggtctct gtgcatgacg cagtgtcaga
     5461 tatgaagctc cttgcgtcgg agacgatcct gaataatctg atttggaaga agctcgaaat
     5521 ttgtcatgcg atgaagcttg aaggacataa agtcgctgaa ggctttcaag ttgtcacggt
     5581 gcacgtgatc gatgccagca tgctgccgat gtctttacag tcatttgcag gcgattgctt
     5641 aattaagaaa gcacttgaaa ttgcgccgat ggtcgactag cagggaggag ttaggcgagc
     5701 cggcattctg gacaagtagg tggctcgtcc ggtgcctcac actcttcggt ggtcaaggag
     5761 aaaagctgtt cacactgtcg acatgggcga atctcgaagt aggccgttcc cttgtcatca
     5821 atatgggtcg gcaggagcaa ttcgagaggg tcatacccaa tcacgcctcc gtcatggaat
     5881 ttgacgatgg ccaaccggtc attaaagacg tcgaaggtac cttcttgccc ttttctggcg
     5941 aggacatgtc cgacaacgaa tacaggctgt cccttacgtt tcgtgcgaag gtctcgtaaa
     6001 gtatgttggg tgtccgtcgc gcaagaaaat ttacctgtag agctagttcc gaaggttggc
     6061 gttggcatat gactgtcatc cgcgtaagta atataatacc cctgtatcat acgagtaaaa
     6121 atggcgtcaa ggaaggcatg acgcacagac aaggagtgtg aagatggctg aggtgacgat
     6181 ttaccagaaa ccaacctgta gcacatgccg tcaggcggtg cagctattaa aagagagtgg
     6241 caagccgttt acaacggtca actattatga gaggcccttt tcgaaggcgc aactcaagag
     6301 cctgttgaaa aaagccgggc tctcaccgaa agaaatactg agaaccaaag aggacatcta
     6361 caaggagctc gggctagcca aaaaggatct ttcggatgat gaatggctgg aagtcctgat
     6421 cgcccatccg gatctcattc agcgcccgat tgtcgaaaaa ggagaaaagg tgatcttggc
     6481 gagaccggcg gaatctgtga aagaactgtt gtagttgttg ggcctacgtt tcatattctc
     6541 cactcgcaac aattttccct gatcctgcat gagatcgttt tggcggggca ctggccaccg
     6601 gtcctcgttt cacaacgttg ccccctatcc ccttggcctc atagagggcg tgataggccg
     6661 atttaatcac caggctcagc gtcgcgttgt catttaattt ttcccccacg ccgatactga
     6721 gtgtcaccgg aagctctcgg tcgcgtgatc cagccttctt gacccctccc tgcgtttccc
     6781 agacccgatc ctgtcctcgg aggaataggc taatgacatc gatcgctttc cggacacctt
     6841 ccaacgtact caaggtttct gtggcacatt ttcccggaaa caggacggtg aggtcctctc
     6901 cggtcgtccg aaagatttgc gcattaacac aggcggcctg aattctgggg gcaactagtt
     6961 tcagaatctg ttcgctgaca ggcttcccgt gcgtattggc atagtgagtg agctggtcga
     7021 tgctgatgac cgcgatggaa aattgttttc caagttgtct gattgcctcc tcataggcca
     7081 ggcgaccggg aatgccggtc agctcatcgc ggtaggtccg ttgatagaac gattgaagta
     7141 aggtgacgaa gaggatgagc ccggccgcca taaagaaatt tgtcgattgc cagccatagc
     7201 ggctcgtatg ataggccgcg aatacggcaa agagtaccca aattgcccct ccttccagag
     7261 ggtcgcgatg aagagcgaat cgaatgaagt ggagcagcag tgctaaggca aaggccacga
     7321 gtgccggctg tggaatcggt gtccaatccg tatacagcgg gggaatgtac tcccgtgtga
     7381 aggacgaggc aaggtcatgc tggtcgggaa ggcagagcca gaggagtaga aagggttgga
     7441 ccaacacgag cagcacacgc atcacgccgc gaagtgtcga gagggaatct tctttgagga
     7501 tggaaaaggc caaaagattg agcgggacaa gaaaggctgt cacggccacc atcgtctggc
     7561 ctggggctgc ctgatctgca tcggtgactg ggaaaaatac cagggctcga tctgcaagtg
     7621 ccagaacgag caaggagagg atcatccgac tgcttgaaaa ataccagcca aagatgagcc
     7681 caaacatgag gacgatatac ggcagggctg caacgggttg ttgtaaccac attgggagcc
     7741 cctgaggacg gaggaatcca atcgcgagga gaataatgag ccctccgggg aagagtactg
     7801 cgaggaggga tttcccgatc agatgtggca tagattgcat ttgtgtaccg acggggggcc
     7861 tctagtggag tgccagatcc ataccaacag gggcttggct gtacaggaat attctagaaa
     7921 gcaaaacgca aatcacactg taaaataagg gcttttcagc tgaaatctga cttccgccag
     7981 tcaccttgct ggcagccgtg gccgcttggg caaagcaggg gcatttttga acagtcggtc
     8041 gaggggtatt gtcaggcaag gggaatgagg gacggcttgt tcgcttgatg cattggaggg
     8101 acgtcctggg ccatccgaag aacaccggat gttgggtcta cgctgatcgt gacccctcca
     8161 cccaacactt ccaacagtag atcaccagcc aattgccttc gccagccatg aagaataggt
     8221 atatcaaggg cggtccgatg ttgcttggct tccacgagcg tttgtaggtc ggcactggtc
     8281 gcaagcattg tgggggcaat tcccttctct gctgcacggg ctttcaatac ggcttgcaga
     8341 agttcgacga tgccggttga atcaggatcc ggtttccgct ccgatgggac cttgggccat
     8401 gccgatggtg gaagagccaa ggccgaggtg atgatggcca gtagctgctc tccgtgtcga
     8461 tcgatctccg aggaatggac gccacgcaga ccacga
//
LOCUS       DATIPF010000335         4517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1392444, whole genome shotgun sequence.
ACCESSION   DATIPF010000335 DATIPF010000000
VERSION     DATIPF010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392444"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1567
                     /locus_tag="VLL06_08405"
     CDS             <1..1567
                     /locus_tag="VLL06_08405"
                     /inference="COORDINATES: protein motif:HMM:NF027510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HSS31025.1"
                     /translation="GLALSAGVGTLIDNGNGTWSYTPAANDDTAVAFSYTITDGTATV
                     AGSATHDIIPVNDAPVNTVPGVQTVTENTPLALGGISVNDVDGNVSTVQLAVTNGTVT
                     VNLAGGATISLGSNGTNTLTLSGNQIQINAALGTLTYQGTLNYNGPDTLTVTTTDSNA
                     VTDMDTVAITVTPVNSAPTLVTNAGATVFQGLSDPITSGELQVTDANNTPAQLIYTVT
                     TPPVNGHLELLTAPGVAIMSFTQAEIDAGQVLYVNNGANSTSDSFIFTLSDGAGGTIG
                     ATTFNITVTPFFPPPVPVPVPVPPPLPIPVPTLLPPVITPPPGGGVVTPVLPPPVPLW
                     VQGGGSSDGAKGGGTTDEAVRRVESSGKTFIRVEQPVVGLDEPAILSLEPLSNPVKTI
                     VGMSHKLAEHLTRLADDLERAAQEREHQAHLLGRVASVSGFALSAGFVAWILRGGSLL
                     ASFLVSMPAWRHFDPLPVLGGGEGNRRKLDRKAREEQKEESQQFRGLDRVLNSSAKAV
                     TQQKTERMRRPKP"
     gene            1567..3486
                     /locus_tag="VLL06_08410"
     CDS             1567..3486
                     /locus_tag="VLL06_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS31026.1"
                     /translation="MRVTPLVWISCGLVSLTISVLMAGDWLFDLAPNHDRQIFEYRRD
                     LAESLAVQYSALAERDQIETVKFAMETLAKRIPDILSLALVQNSGMVVAQIGDHARVW
                     VQPPGEESTLDFLQVPIFSGEQRWGVLQVAFRQIQANVSEVQRFLTDPWVRFLAFVSV
                     VGFVGYLFFMKRTLRQLDPSGIIPSRVKAALDALAQGVVMIDTRDFIVLANDVFCQAV
                     GKPVTSLIGSDLSTLSWAPSAPSAPVLVHPWTEAIMDKLPHSDRALLLKLPDGESRKF
                     IVNTVPIMDDASTVRGALVSFHDVTELDRANVSLREANSELELSRFQILEKNQELETT
                     NTSLHVEMNERKKVQAEKEELHQKLVQASRQAGMADVASNVLHNVGNVLTSINVSTDI
                     LLKTLKKPMVGDVCRIASMFHEHQNNLEAFLTQDSKGKQIPAYLGMVAESLSGSHQTI
                     QSELDSLVIKIDHVKQVIMSQQDIAHADAVREATAVEDLMEQALMMGMPEPEKYGIRV
                     VREYASVPTIMTDRHHVLQILVNVITNAKNAMVEYPTTDHCLTVRILLPTDRKGSVRF
                     EVIDTGGGITAENFPRLFTQGFTTRKAGHGLGLHSAAISAKNLGGILQAQSAGAGCGA
                     TFMLDLPLTAMDAAA"
     gene            3483..4385
                     /locus_tag="VLL06_08415"
     CDS             3483..4385
                     /locus_tag="VLL06_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HSS31027.1"
                     /translation="MKNNPTAGNRRILVIDDNVSIHEDFRKILLPPKGADALDQARVA
                     LFGETLSLPPQERYELEFASQGREGFGLVDHALREGKPYAMAFVDMRMPPGWDGLETI
                     EHLWYVDPALEIVVCTAYSDHPWEDVSRRIGNTDKLLILLKPFNSIEVIQLANSLTKK
                     WNLARAVEYEIDILSSSVIKRTTELQEANGRLQGNISRRLTETVQGQSPEEAAAMFRQ
                     KEEFLAIISHEILTPMNELLDKVRLLLDDSLNPGQRESATTIQRCAQDLLALLKDMHE
                     FMAGKPSGGVVNVAGDPINANKRA"
BASE COUNT         1039 a         1235 c         1315 g          928 t
ORIGIN      
        1 aggcctggcc ctcagcgccg gtgtgggcac cctgatcgat aacggcaacg gcacctggag
       61 ctataccccc gccgcgaacg acgataccgc cgtggcgttc agctacacca tcaccgacgg
      121 aacggccaca gtggcaggca gcgcgacgca cgacatcatc ccggtcaacg atgcaccggt
      181 gaacacggtg ccgggcgtgc agacggttac ggagaatacc ccgctcgccc tcggtgggat
      241 cagtgtcaac gatgtggacg gcaatgtatc gacggtgcaa ctcgcggtaa cgaacggcac
      301 agtgacggtc aacctcgcgg gtggcgccac catcagtctg ggcagcaatg ggaccaatac
      361 cctcacgttg tctggcaacc aaatccagat caacgcggcc ctgggcacac tcacctatca
      421 agggaccctc aactacaacg ggccggatac gctgacggtg acgaccaccg acagcaatgc
      481 ggtgacggac atggacacgg tggcgatcac cgtgacgccg gtgaatagcg ccccgactct
      541 ggtcacaaac gctggcgcga ctgtcttcca aggattgtcc gatcccatta catcgggtga
      601 attgcaagtg acggatgcga acaacacgcc agcccaactg atctacacag tgacgacacc
      661 tcccgtgaat ggccatctcg aattactcac tgctcctggc gtggcgatta tgagctttac
      721 gcaagcggag atcgatgcgg ggcaagtgct gtacgtcaac aacggagcca atagcaccag
      781 tgacagtttc atctttacgc tcagcgacgg tgcggggggc acgattgggg caaccacctt
      841 taatattacg gtcacgccat ttttcccccc gcccgtgcca gttcctgtcc ctgttcctcc
      901 tcccctcccc atccccgtcc cgaccctcct acctccggta atcacgcctc cgcccggcgg
      961 cggtgtggtg acgccggtgt tgccaccacc agtaccgctg tgggtgcagg gtggagggtc
     1021 cagcgatggt gcgaaggggg gtgggaccac cgatgaggca gtgcggcgag tggaaagctc
     1081 gggcaaaaca ttcatcagag tggaacagcc ggttgtcggg ctcgatgagc cagcgatcct
     1141 ttcgctggag ccactctcga acccggttaa aacgatcgtg ggcatgagtc acaaacttgc
     1201 tgaacattta accaggctgg ccgacgatct cgagcgggcc gcgcaagagc gtgaacacca
     1261 ggcacacctc ttgggccgag tcgcatcggt atcggggttc gccttatctg ccggattcgt
     1321 ggcatggatt ctgcgcgggg ggtctttgct ggcaagtttt ctcgtgtcga tgccggcctg
     1381 gcgccacttt gatccactgc cggtgctggg tgggggtgaa ggaaatcgac ggaaacttga
     1441 tcgcaaagca cgtgaggagc agaaggagga gagccaacag ttccgcggct tggatcgcgt
     1501 gttgaactcg tcagcaaaag cggtcactca gcaaaagacg gagcgcatga ggaggccgaa
     1561 gccataatgc gcgtgacgcc gctagtctgg ataagttgtg gactggtcag tctgacgatc
     1621 agtgtgctga tggcgggtga ttggctcttc gatttggcac caaaccacga tcgccaaatt
     1681 ttcgaatatc gtcgagattt ggccgagtcg ttggccgtgc agtactcggc gctggccgag
     1741 cgtgatcaaa tcgagaccgt gaagtttgcc atggagacgt tggccaagcg gattccagat
     1801 atcttgtccc tggcactcgt ccagaacagt gggatggtcg tggcacagat tggtgaccat
     1861 gcgcgagtct gggtccagcc gcccggtgaa gaatctaccc tcgattttct ccaagtcccg
     1921 attttttccg gtgagcaacg ctggggcgtg ctccaggttg catttcgaca aattcaagcc
     1981 aacgtctcag aagttcaacg gttcctcacg gatccatggg ttcgcttcct ggcgtttgtc
     2041 agtgtcgtgg gattcgtcgg gtacctcttc ttcatgaaac gcacgctccg tcagctcgat
     2101 ccctcaggca ttattccctc gcgtgtgaaa gccgcgctcg atgcgctcgc gcaaggtgtg
     2161 gtcatgatcg atacgcggga tttcatcgtc ttggcgaacg atgtgttctg ccaggctgtg
     2221 ggaaagccgg tgacctcgtt gatcggatcc gacctgagca ccctgtcgtg ggccccgagc
     2281 gctccatccg ccccggtctt ggtacatcca tggacagagg caatcatgga caagctgccc
     2341 cattcagaca gggcccttct cctgaagctt cctgatgggg aatcgcgaaa gttcatcgtc
     2401 aacaccgtgc cgattatgga tgacgcatct acggtgagag gggccctggt ctcctttcac
     2461 gatgtcactg aactcgatcg agcgaatgtt tctctccggg aggcgaacag cgagttggag
     2521 ttgtcccgtt tccaaattct ggagaagaac caggaacttg aaacgacaaa tacgagcctc
     2581 catgtggaga tgaacgagcg gaaaaaggtt caggcagaga aagaagaact gcatcagaag
     2641 ctcgtgcagg catcgcgcca agccggtatg gcggatgtgg cgtcgaatgt gctgcacaat
     2701 gtagggaatg tcctcaccag cattaatgtg tcgacggata tattgctgaa gacgctcaag
     2761 aagccaatgg taggagacgt ttgccgaatt gcctcaatgt ttcatgagca tcagaacaat
     2821 ttagaggcct ttctgacaca ggactccaag ggcaagcaga ttcccgcata tctgggcatg
     2881 gtggcagagt cgttgagcgg gagccaccag accatccaga gcgaactcga ctccctcgtg
     2941 ataaaaattg accatgtcaa gcaagtgatt atgtcgcagc aggatattgc gcatgcagac
     3001 gcagtccgcg aggcaacggc agtcgaggac ctgatggagc aggctctcat gatgggaatg
     3061 cccgaaccgg aaaaatacgg catccgagtc gtgcgagagt atgcctccgt tcccacgatc
     3121 atgacggatc gacatcatgt ccttcagatt ctggtgaatg tgatcaccaa tgccaagaac
     3181 gccatggtcg aatatcctac gaccgatcat tgcctgacgg tcagaattct gttgcctacg
     3241 gaccgcaaag gatccgtgcg atttgaggta atcgatactg gtggcggcat tacagctgaa
     3301 aacttcccac gcttgtttac tcagggcttt acaacgagaa aggccggaca tggcttgggc
     3361 ctgcacagcg cggccatttc cgccaagaat ctgggcggaa ttctacaggc ccagagtgcg
     3421 ggagcaggat gcggtgcgac cttcatgctc gatctcccct tgactgcgat ggacgctgcc
     3481 gcatgaagaa caatccgacg gctggtaatc gacggattct ggtgatcgac gacaatgtgt
     3541 ccatccatga ggactttcgt aaaatcttgt tgccaccgaa aggtgctgac gcactcgatc
     3601 aagcgcgcgt ggccttgttc ggagagaccc tgagtcttcc gccacaggaa cgctatgagc
     3661 tggagttcgc gagtcagggg cgcgaagggt ttggtctcgt cgatcatgcc cttcgggagg
     3721 gcaaacccta tgcgatggcc ttcgtggaca tgcggatgcc accgggatgg gacggcctcg
     3781 aaacgattga acacctctgg tatgtcgatc ctgcccttga gatcgtggtc tgcaccgcct
     3841 attccgatca tccgtgggag gacgtgagcc gccgaatcgg gaacaccgat aagctgctca
     3901 tcctattgaa acccttcaac agcattgagg tgatccaact ggctaattcg ctcacgaaga
     3961 aatggaactt ggcacgtgcc gtcgagtatg agattgacat tctttcgtcc agcgtaatta
     4021 agcgaacgac cgaattacag gaagcgaatg gacgccttca gggaaacatc tccaggaggc
     4081 ttacggaaac ggtgcagggt caatctccgg aggaggccgc cgctatgttt cgtcagaaag
     4141 aagagttttt agcgatcatt agccatgaga tcctcacacc catgaatgaa ttgctcgaca
     4201 aggtgcgtct gctgcttgac gattcgctta atccaggaca gcgagagtcg gccacgacga
     4261 ttcagagatg cgcacaagat ctcctggccc tcctcaagga catgcatgag ttcatggctg
     4321 ggaaaccttc agggggagtc gtcaatgtcg ctggtgatcc gatcaacgcg aacaagaggg
     4381 catgaataat catggagacc aatcgtaaaa aaccacgcat tttggtgatt gatgacaatc
     4441 gggatattca cgaggatttt cgcaaaatcc tccaagcgaa cgccgaggac gagagactcg
     4501 atcaagcacg ggcggcc
//
LOCUS       DATIPF010000336         6140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1742485, whole genome shotgun sequence.
ACCESSION   DATIPF010000336 DATIPF010000000
VERSION     DATIPF010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742485"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VLL06_08420"
     CDS             complement(<1..289)
                     /locus_tag="VLL06_08420"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247209.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HSS31028.1"
                     /translation="MPKHELILLKNMMQPGYTGSLQEYERTGGYQALRKVIGKVTPTE
                     VTMAVMKSGLRGRGGAGFPTGVKWGFLPKDYEGPRYLCCNADESEPGTFKDR"
     gene            complement(339..869)
                     /gene="nuoE"
                     /locus_tag="VLL06_08425"
     CDS             complement(339..869)
                     /gene="nuoE"
                     /locus_tag="VLL06_08425"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HSS31029.1"
                     /translation="MLKEKYQKEIDEILSRYPVKRSALIPLLYLAQQDEGFVSEAAMK
                     EIAGLLRLTPPQVYETATFYTMINLKPVGKFHLQICKSLMCALAGSDTVIGWVKTKLG
                     IGPGETTADGLFTLTAVECLAACGTAPMMQVNEDYYERLTEDTFDRILTDLKKDGTCS
                     LKSGPFMWPEPVNNKG"
     gene            complement(977..2728)
                     /gene="nuoD"
                     /locus_tag="VLL06_08430"
     CDS             complement(977..2728)
                     /gene="nuoD"
                     /locus_tag="VLL06_08430"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HSS31030.1"
                     /translation="MQALLDSLKTKFPDAILATRVDAARGETAVSVAAAHLVDIARYL
                     HDAPEGAFDHLTDICSVDYPEDQLRFEIVYHLHSLPLGQRLRLKTRIAEDDPTIASVT
                     GVWKGAEFLEREVYDMMGIHFSGHPDLRRILLPEDYAEGYPLRKDFPTEGRGWRSQFD
                     FIPRFDEPSRDVPESEVTEEQRNTFRADPGVSGSQRKEELLLNMGPQHPSTHGVLRVV
                     LVLDGERVVKATPDLGYLHRGVEKLAEGLAYMQMIPHTDRLDYVCAMSNNYAYVRAVE
                     KLLEITVPIRAEYVRTIVAEMQRIIGHLFWLGTQALDIGAMTVFFWTFREREVLLDMF
                     EKLCGARLTLNYYRIGGVDSDFTPELVQRMKAFLEVFPEKVKEYDSLIASNRIWLGRT
                     RNVAVLSAEDAISFGCTGPVLRGSGVAYDVRKAEPYGVYDKVEWDVPIGKNGDTYDRY
                     WIRMEEMRQSARIIAQCFDQMPDGPIMAEAPQYIPPPKELVMRDMESLIHHFIIYTQG
                     IKPPKAETYCASEAPKGELGFFIVSDGSARPYRLKIRSPSFVHMGAFDHMARGYLISD
                     IITIFGTYDVVMGECDR"
     gene            complement(2808..3287)
                     /locus_tag="VLL06_08435"
     CDS             complement(2808..3287)
                     /locus_tag="VLL06_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HSS31031.1"
                     /translation="MSLLERQFEANILTTNLDALVNWSRKSALFPMTFGLACCAIEMI
                     ATVSSRYDMDRFGAGVFRASPRQADLMIIAGTVTRKMAPVIRRIYDQMPEPRYVISMG
                     SCATSGNHYNSYAVVQGVDQIIPVDVYVPGCPPRPEALLDGLLKLQEKIQREKVFVR"
     gene            complement(3526..3897)
                     /gene="ndhC"
                     /locus_tag="VLL06_08440"
     CDS             complement(3526..3897)
                     /gene="ndhC"
                     /locus_tag="VLL06_08440"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HSS31032.1"
                     /translation="MGNTAIPENYIPILLFFGIALIGVAGTLLVGSFIRPSRPYRAKM
                     APYESGSPLFSDARIQFPMRYYIIAMLFVIFDVEVIFMIPWAVRFQSLGLLGLIEMIV
                     FLLILLVGFWYAWKKGALEWD"
     gene            complement(3975..5153)
                     /locus_tag="VLL06_08445"
     CDS             complement(3975..5153)
                     /locus_tag="VLL06_08445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HSS31033.1"
                     /translation="MTTGHPELIAAIASEIASSGPIPFARFMELALYHPRFGYYMCQP
                     NRADRERIGWSGDFYTSSDVYPILGHALAAQARQMDELVGCPTPFTIVEMGAGKGLLA
                     RDCLSAIHAQQESFASRVRYILIERSPVMRELQRQNLGPWLSTPGLVTWVEGLDGMAP
                     QSVTGLFFSNELVDAFPVHRIQVTAGGTEELYVDYRDGQFVEYLKPPSTTLVGQYLER
                     LHTTWAEGYRTEVNLQALDWMEQVAQRIDRGFVVTIDYGHTAQDLYGPERKNGTFLCY
                     SQQLTNEDPFIRVGEQDMTAHVDFSSLAAVGEEQGLHVTGFTNQMSFLMGLGVERMIE
                     ELEPESPEFYAGIHLLKPDGMGSTFKVLIQHKGIHHPELDGLKFKPFFGSALAAHSAG
                     "
     gene            complement(5827..>6140)
                     /locus_tag="VLL06_08450"
     CDS             complement(5827..>6140)
                     /locus_tag="VLL06_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HSS31034.1"
                     /translation="YGQKDPLIEYKREGYDLFAGMMERVKADALDRLFHVQAVRNEAQ
                     PTAPPPPPPVISRPLPPLILNRGEEPVAPQTAQRSDEKVGRNDPCPCGSGKKYKKCHG
                     V"
BASE COUNT         1349 a         1764 c         1649 g         1378 t
ORIGIN      
        1 ggcggtcctt gaacgtgcct ggttcgcttt cgtccgcgtt gcaacataga tagcgcggac
       61 cctcatagtc cttgggcaga aatccccatt tcacaccggt cggaaagccg gcgccgccgc
      121 gcccccggag acccgacttc atcaccgcca tagtgacttc ggtcggcgtc actttcccta
      181 tgaccttgcg gagcgcttgg tagccgcctg tacgttcgta ttcttggagc gaaccggtat
      241 agccgggctg catcatgttc ttgagcaaga tcagttcgtg tttaggcatc tcaacctcaa
      301 ctcgcgaggg atgaagctat cccctcactt cttacatctt accccttatt gttaaccggt
      361 tcaggccaca taaacgggcc ggacttgagc gagcaggtcc cgtccttttt cagatcggtc
      421 aggatccggt cgaatgtgtc ttctgtgagc cgttcgtagt agtcttcgtt gacttgcatc
      481 atcggcgcgg tgccacaggc ggcaaggcat tcgaccgccg tgagggtaaa gagtccgtcg
      541 gccgtcgtct cacccggtcc gattccgagt ttcgtcttga cccacccgat aacggtatcc
      601 gacccggcca gcgcgcacat cagggattta caaatttgga gatgaaattt ccccaccggc
      661 ttaagattga tcattgtata gaacgtggct gtttcataga cttggggcgg ggtcagacgc
      721 aaaagccccg cgatctcctt catcgccgct tcggacacga aaccctcgtc ctgttgcgcc
      781 aagtacagca atgggatcaa cgccgagcgc ttcaccggat accggctcag gatttcgtca
      841 atctctttct gatacttttc ttttaacatc accctctcgc tgtgaccatg gcttgagaga
      901 gcctctattg cgcgcatcga ccgagcacct tctgagcgtg cgcggtctgc gagcagggaa
      961 gcttctcaag tcatggtcat ctatcacatt ctcccatcac gacgtcgtaa gtcccgaaga
     1021 tcgtgatgat gtcggaaatc agatagcctc gcgccatgtg atcgaaggcg cccatatgaa
     1081 caaatgaggg cgagcgaatc ttcagtcggt agggacgggc gctgccgtcg ctgacgatga
     1141 aaaatcccaa ttcgcctttt ggcgcttctg acgcacagta ggtctcggcc tttggcggct
     1201 tgatcccctg agtgtaaatg atgaaatgat ggatcaaact ttccatgtcc cgcatgacca
     1261 gctccttggg cggcgggata tattgcggcg cctcggccat gatcggtcca tccggcatct
     1321 ggtcgaaaca ttgtgcgatg atcctggcac tctgtcgcat ctcttccatg cggatccagt
     1381 agcggtcgta ggtatcgccg tttttgccga tcggcacgtc ccattcaact ttgtcgtata
     1441 cgccgtaagg ctcagccttg cggacatcgt atgcaactcc agacccgcgc aagaccggcc
     1501 ccgtacatcc gaaactgatc gcgtcctcgg cggaaagcac ggccacgttc ctcgtgcggc
     1561 cgagccagat gcgattcgat gcgatgagcg agtcgtattc cttgaccttc tcgggaaaga
     1621 cttcgagaaa cgccttcatc cgttggacca actcaggcgt gaaatcgcta tcgacgccgc
     1681 caatgcgata gtaattcaac gtcagtcggg ctccacagag cttttcgaac atgtccaaca
     1741 gtacttcgcg ctcccggaag gtccagaaga acacggtcat ggcgccgata tcgagggcct
     1801 gcgtgccgag ccaaaaaaga tgcccaatga tacgctgcat ctcggccacg atggttcgga
     1861 cgtactcggc acggatcggc accgtgatct cgaggagttt ttccaccgca cgcacgtacg
     1921 cgtagttatt ggacatggca cagacgtagt cgagccggtc cgtgtgcggg atcatctgca
     1981 tgtatgcgag cccctcggcc aatttctcga cacctcgatg cagatagccg aggtctggag
     2041 tagctttcac aacccgttca ccgtccaaca caagcacgac acgcaacacg ccgtgggtgc
     2101 tcggatgctg tggccccatg ttcagcaaca attcttcttt ccgctgggaa ccggacacgc
     2161 cagggtcagc gcgaaaggtg tttctctgct cctcggtgac ttcgctctca gggacatcgc
     2221 gcgagggttc gtcgaatctc ggaatgaagt cgaactggct gcgccagccg cgcccttcgg
     2281 tcgggaagtc ttttctcagg ggatatccct cggcataatc ctccggcaac aaaatgcgcc
     2341 ggagatcggg atggccggaa aaatgaatgc ccatcatgtc atacacttcc cgttccagga
     2401 actcggctcc tttccagact ccagtgaccg acgcgatcgt cggatcgtcc tctgcaatac
     2461 gcgtcttgag gcgaagacgc tgcccaagtg gaagcgagtg caagtggtac acgatttcaa
     2521 accgcagctg atcttcggga tagtcgaccg agcagatatc ggtgagatga tcaaacgctc
     2581 cttccggcgc atcgtgcaaa tagcgcgcaa tatccaccag atgcgcggcg gccacggaaa
     2641 ctgcggtttc gccacgtgcg gcatcgacgc gggtcgcgag gatcgcgtcg gggaattttg
     2701 tctttaggct gtcgagtaga gcttgcatcg ttcttgttcg agggaggtgc gagagacgca
     2761 caatgcgtcg atcatgcaca tctcgatttt cttcttcatc tcacggctta tctcacaaag
     2821 actttctccc gctgaatctt ttcctgtaat tttaacagcc catcgagcaa cgcttccggg
     2881 cggggcggac agccgggcac atagacatcg actggaatga tctggtcgac gccttggacc
     2941 actgcataac tgttgtagtg gtttcctgag gtggcacagg aacccatgga aatgacatag
     3001 cgtggttccg gcatttggtc gtagatgcgc cggatcaccg gcgccatctt gcgcgtcacc
     3061 gtaccggcga taatcatgag atcggcttgc cgtggcgagg cccgaaagac gccggcgccg
     3121 aaacgatcca tgtcgtaccg cgacgacacc gtcgcaatca tttcgatggc gcagcaggct
     3181 aggccgaacg tcatcggaaa caatgccgac ttccgagacc aattcacgag cgcgtcaaga
     3241 ttggtcgtca gaatattcgc ctcgaactgc cgctctagaa ggctcatctg ttctactcca
     3301 ccattaccag aaggggattg tcgtgatgcc cttcattgcg tgcgttatcc caatcctccc
     3361 atctcacttt caagggtagc ctggttgatc ctcgattgcg cgcgtcgaac gagcacattc
     3421 ctatcgtgcg cgttccgcga gcaggaggac gaccaggctg cccttcccat ccttccgagg
     3481 ccgcgcgctg cgcgagcatg gagcccgtcc tgcacacccc tacaatcagt cccattcgag
     3541 cgctcccttc ttccaggcat accaaaatcc gaccagcagg ataagaagaa agacgatcat
     3601 ttctataagc cccagcaggc caagtgattg aaatctgacg gcccagggga tcatgaaaat
     3661 gacctcgaca tcgaaaatga cgaacagcat cgcgatgatg tagtagcgca tcggaaactg
     3721 gatgcgcgcg tcggaaaaca atggactccc gctctcatag ggagccattt tggctcgata
     3781 gggacgactc ggtctgatga acgagccgac aaggagtgtg cctgcgacgc cgataagggc
     3841 aataccgaaa aacaacagaa tcggtatgta attctccgga atggcggtgt tacccatacg
     3901 ggctccatcc agcgcatcgc gtgaatcgtg aaacgttgtc caatcggtac aaggtcatcg
     3961 cgctacccta tcagttatcc tgccgaatgg gccgccagcg ccgaaccgaa gaacggtttg
     4021 aatttcaagc cgtccagttc cggatgatgt atgcccttgt gttgaatgag caccttaaac
     4081 gtactgccca taccatccgg cttcaggaga tgaatcccgg cataaaactc tgggctttcc
     4141 ggctccaatt cctcgatcat cctttcaacg ccgaggccca tcaagaaact catttggttc
     4201 gtgaatcccg tcacgtggag gccttgttcc tcaccaacgg cggccagact cgaaaagtcc
     4261 acgtgggcag tcatatcctg ctcgccgact cgaatgaatg gatcttcgtt ggtcagctgt
     4321 tgagaataac agaggaacgt gccgttcttc cgctccggcc catagagatc ttgtgccgta
     4381 tggccataat cgatggtcac cacaaatcca cgatcgatcc gctgcgcgac ctgttccatc
     4441 caatccagcg cttggagatt cacctcggtt cgatagccct ccgcccaggt ggtgtggagt
     4501 ctttctagat actgaccaac cagggtcgtt gacggaggtt tcaggtattc cacaaactgc
     4561 ccgtctcgat agtctacata gagttcttcg gttcctccag ccgtgacttg aatccgatgc
     4621 accggaaaag catcgaccag ttcattgctg aagaacaggc cggtcacgct ctgcggggcc
     4681 attccatcaa ggccttccac ccaggtgaca agacccggtg tgctgagcca gggacccagg
     4741 ttctggcgct gcagctcccg catgacaggg ctccgctcga ttaagatata acggacacgc
     4801 gacgcgaagc tttcttgttg agcatggata gccgacagac aatcccgtgc taataggcct
     4861 tttcccgcac ccatttcgac gatcgtgaag ggagtgggac atccgactaa ctcgtccatt
     4921 tgcctggctt gcgcggcaag cgcatgaccg agaatcgggt acacatcgga actggtatag
     4981 aaatcaccgg accagccgat acgttcacga tccgctctgt tcggttgaca catgtaatag
     5041 ccaaatcggg gatggtagag cgccagttcc atgaacctgg cgaaggggat cggacctgag
     5101 gaggcgattt cggacgcgat ggcggcgatg agttcagggt gcccggttgt cacaagtaac
     5161 tcctcctccc atcgacaata gacaaaaagt gacacccata cccgatgggg acacttcacc
     5221 ctccaaaata tgggcttaat gagatatagc gtagcgctca aaggcgtgct aagtcaaggt
     5281 gaaataagga agaaagcttg tcgctggccg agttcaagag gctgaaccta cctctgttca
     5341 gccacccgag ccgatcgaca gtgaagctga agcaagaact gccgaataag cacgattcat
     5401 ttctcccaaa cccatccggc aatacagtga acgtaccgaa ggagagatta agttgaaccg
     5461 aaagaccccc tggtcattga aatcaacttt cctgaatccc gtattccaca ggatgttgtc
     5521 attccctcct ttgttgccga cggtccgaca cttcacgatg agaagccgac gttcctatct
     5581 cgtctcgcga ctgtgctgag ctagtccgcc agagtctgcg agtttgacct agcttcgact
     5641 tccatctcac gacgctgaca ttgttcctca gcttttcgtt gcggctcgct gaaagcgagt
     5701 ggtgccgatg ttcgacgttc cgttcatgtg tagcatgtta cggaacaagt tttggcggca
     5761 agcaactgtg agatgtagaa cggtcgcatt gacgcgtggg agagaggcgg acgcagtgat
     5821 cctactctac actccgtggc acttcttgta ttttttcccg ctgccgcagg ggcatggatc
     5881 gttgcggccg accttttcgt cgctccgctg agcagtctga ggtgcgactg gttcttctcc
     5941 gcgattgagg atgagtgggg ggagtggtcg tgagatgact ggtggcggcg ggggaggcgc
     6001 cgtgggttgt gcttcgttcc ggaccgcctg cacatgaaag agccgatcaa gcgcgtcggc
     6061 cttgactcgt tccatcatgc cggcaaagag atcgtagcct tcgcgcttgt actcaatgag
     6121 cggatctttc tgcccatagc
//
LOCUS       DATIPF010000337         3787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_155440, whole genome shotgun sequence.
ACCESSION   DATIPF010000337 DATIPF010000000
VERSION     DATIPF010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155440"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(552..1013)
                     /locus_tag="VLL06_08455"
     CDS             complement(552..1013)
                     /locus_tag="VLL06_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31035.1"
                     /translation="MCRKIPWWLTFVLIILMTLSGCDKNRQPLQSQSNSGSVMDLWHT
                     YTHCNRSADLDEMRDDAQQLIQAANTFDSADDSIPLQRDEPAPSGPTTRFSVDPGAMA
                     AACTLHTGQAAQKMGHLNIAREMFQLVVLRFHQSRYQYYTTQARLGLEHLT"
     gene            complement(1094..1474)
                     /locus_tag="VLL06_08460"
     CDS             complement(1094..1474)
                     /locus_tag="VLL06_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS31036.1"
                     /translation="MERNNKSLLVVEDDRDLRYLLSVRLVSAGYIVYGAENGLEALAQ
                     MEKHSVDAVLTDYHMPKMDGLEFLSISRAKWPGIPVVVYSAEQDYMAHEAMDRDAFAW
                     VRKGNDFTMLLEILAAAVQQSVHA"
     gene            complement(1827..3179)
                     /locus_tag="VLL06_08465"
     CDS             complement(1827..3179)
                     /locus_tag="VLL06_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSS31037.1"
                     /translation="MLRQSRVMVVDDDADTLSLLREVVAKEGYQVETAEDAETALQKL
                     SEWQPDLVITDIHMPGKDGLALLAAVREKAPDILVILLTAYGSLKTAVDAIKAGAFDY
                     LSKPFVVDDIRLVVRRALEHKKLVRENRSLRDQLRERYRFDNLVGSSAGMVAVYKLIA
                     RVAETDSTVLIQGESGTGKELIARAIHANSTRSNGPFVAIDTGSLAETLLESELFGHE
                     RGAFTGAVASKKGLLEQAHLGTCFLDEIADLSPVMQSKLLRSLQEREVRRVGSETTRG
                     IDVRIIAASKKDLKPLVEAGTFRDDLFYRLEVVTIVLPPLRDRIEDIPLLSQNFVEKY
                     GRTREKPVTGFSPETIPLLTRYSWPGNVRELEHVIERAIALTPHPIITPEDLPETIRT
                     TSVQHQARARGWDTLEDMEKNYILRVLEAHHQDHGQAAATLGIHRKTLLRKLRQYGQG
                     "
     gene            complement(3179..>3787)
                     /locus_tag="VLL06_08470"
     CDS             complement(3179..>3787)
                     /locus_tag="VLL06_08470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS31038.1"
                     /translation="LAEDPTLSAESRQRLETVEAQIERTSKIIQDMLMYARRPEPIRS
                     PLDLNACVAECLALFGPEFERRKIALVAEWSREVEKVDADVQQLQEVCNNLIENALDA
                     MPAGGTLTVRTSPQAEPEQPHYRTSVKVEFADTGHGIDAEQMAQIFQPFFTTKKARRG
                     TGLGLAIALETVRAHGGQIKVDSHPGAGSRFMIVLPTNGGAR"
BASE COUNT          822 a         1001 c         1029 g          935 t
ORIGIN      
        1 ataacgatat ggtccttctt ttgcgataag aagccgatgg gattgctcct gtcgttcccg
       61 cattgtcatg gcatctttct aggggtgcct ctggtattcc caagcgcaaa tggtttgcca
      121 cagatcagga ggcgaacgaa cattaattat ttcaatgagt ttgaagaatt tgacaagaca
      181 ggaagagaga caaggagtca agtctgagag aacatgttgt ccttggcctg tctaatagag
      241 ccgtaattct acggagattt ggattgtgac attgtgtctc ggaaggataa ctcttcccgc
      301 tggccgatgg agcgttcttc tttcgaggcg gaagagtgtt tagagggccg atcggtgaag
      361 agggtctgaa tgtgataaac ccggagctct gatacaactt aatatgtgaa ttcatagccg
      421 gctggatcga ccacaaggct gatcaccatt ccaagtgtct ccgccattgg taagcgcaag
      481 ccttcgtcta gattcccgtc gacgccttct cacgcgatga ggtcggtaaa ggtgggatga
      541 ctcgctgcat tttaagttaa atgctcaaga cccagtcgag cttgggtcgt gtaatattgg
      601 tagcgtgatt ggtgaaagcg taagacgacc agttgaaaca tttctcgtgc aatgtttagg
      661 tgtcccattt tctgcgcggc ctgaccagta tgaagggtac aggcggcagc catcgcgcca
      721 ggatcgaccg agaagcgggt ggttggccct gatggagccg gttcatcacg ttgaagggga
      781 atggaatcat cggcggagtc gaacgtgttt gccgcctgaa tcaattgttg tgcatcgtcg
      841 cgcatctcat caaggtctgc gcttcggttg cagtgggtgt aggtatgcca caaatccatg
      901 acgctgccgc tgttggattg ggattgcagg ggttgcctat tcttgtcgca tccgctgaga
      961 gtcatcagga tgatcagcac gaatgtgagc caccagggta tctttcgaca cataagataa
     1021 gaccacttgc tccttatacc gaataccgat cattttctct gccagtacaa tcgagggggg
     1081 gcacctccat caattacgca tggactgatt gttgaaccgc tgctgcaagg atctccagaa
     1141 gcatggtgaa atcatttccc ttgcgaaccc aggcgaaggc gtctcgatcc atggcctcgt
     1201 gcgccatata gtcctgttct gcggaataga ccacaacagg aattccgggc catttcgcgc
     1261 gggagataga aagaaactcg aggccatcca tcttgggcat atggtagtcg gtcagcacag
     1321 cgtcaacgga atgtttttcc atttgtgcca aagcttccaa cccgttctcc gccccataga
     1381 ctatgtagcc ggcgctgaca agcctgacag agagtagata gcgcaaatcg cgatcatctt
     1441 cgacgaccaa caggctttta ttgttccgtt ccatagaatt cgtgcctcct tttctggcta
     1501 tccctctcta tggacatcag cgagagcttt ctccaacggg tgtccgtctg gcatattgaa
     1561 agcagactgg accgacaatg gggaggatta gagcaaacag cagaccgtgc tgcgccaaac
     1621 gcagttatcg gcagaaattc tattaagaat caaatcaata gacgattgga taccgacggt
     1681 attgaatgtg gcgcgtcggg gaattatggg aacaagttgt cgcaatggcc ggaagatatc
     1741 cgtgagaatg cgaggaggta tgcggacttc tggctttttg agacaagctg tctcgtgcat
     1801 gaacaggttg gccagtgatt gattggtcag ccttggccgt actggcggag cttgcggagc
     1861 agcgtcttgc ggtggatgcc aagtgtagcg gctgcctggc cgtggtcctg atgatgggcc
     1921 tctagtacgc gcagaatata attcttctcc atatcctcaa gagtgtccca tccgcgagcc
     1981 cgcgcctggt gttgaacgga tgttgtccgg atggtctccg gcagatcttc cggggtgatg
     2041 atggggtgcg gagtcaaggc aatagctcgt tcgatcacgt gctcgagttc gcggacattg
     2101 ccgggccatg aatatcgtgt cagcaaagga atggtttccg gtgagaagcc ggtcactggt
     2161 ttctccctgg tgcgaccgta tttctcgaca aagttctggc tcaggagcgg aatgtcttca
     2221 atccgatcgc gtaatggtgg aaggacaatg gtcacgactt caagccggta aaaaagatcg
     2281 tcccgaaatg ttccggcctc gacgagtggt ttcaagtctt ttttcgacgc ggcgatgatt
     2341 ctgacatcaa ttccacgagt cgtctcgctc ccgactcgcc ggacctcacg ctcctgaaga
     2401 gagcgaagca acttgctttg cataaccggt gagaggtccg cgatttcgtc caggaaacag
     2461 gtgccaagat gcgcttgttc cagcagtccc ttcttgcttg ccacggcccc ggtgaatgcg
     2521 cctcgttcat gcccaaagag ctcggattcg agaagcgttt cggccaacga accagtatcg
     2581 attgcgacga acggcccatt gctcctggta ctgttggcat ggatggcgcg tgcgatcagc
     2641 tcctttccgg ttccactttc tccctggatc agaaccgtgc tgtctgtttc cgcaacgcgt
     2701 gcgatgagtt tatacaccgc caccattccg gcactgcttc ccacgaggtt gtcgaaccgg
     2761 tatcgttcac gtaattggtc gcgcaatgaa cgattctccc gcactaactt cttatgttcg
     2821 agcgcccttc gaaccaccaa tcgaatgtcg tccacgacga acggtttact gagataatcg
     2881 aaggctcccg ccttgatggc atcgacggct gtcttgagcg atccatacgc ggtcaatagg
     2941 atcaccaaga tatccggcgc cttctcccgc acggcagcca gtaaggccag cccatccttc
     3001 cccggcatgt gaatgtccgt gatgacgaga tccggttgcc attcgctaag tttctgtaat
     3061 gccgtctccg cgtcctcggc ggtttcgacc tgatacccct ccttcgccac gacttcgcgg
     3121 agcaggctca gggtatcggc gtcatcgtcc actaccatca cacgagattg tcgcagcatt
     3181 agcgcgctcc tccgtttgtt ggaagtacga tcatgaatcg tgacccagcc cccgggtggc
     3241 tatcgacctt gatctgacct ccatgcgccc gtactgtttc cagcgcgata gccagtccca
     3301 gacccgtgcc gcgtctcgcc ttttttgtcg tgaaaaatgg ctgaaaaatc tgggccatct
     3361 gttcggcgtc gattccgtgg cccgtatcgg cgaattccac cttgaccgac gttcgatagt
     3421 gcggctgttc cggttcggct tggggagagg tccgcacggt cagggttcca ccagccggca
     3481 tcgcatccaa cgcattttca atcaaattgt tacacacctc ctggagctgc tggacatcgg
     3541 catcgacttt ctcgacttcg cggctccact cggcgaccag tgcaatcttc cggcgctcga
     3601 attccggccc gaagagcgca agacattcgg cgacgcatgc attcagatcg agcggagatc
     3661 gaatcggctc cggtctcctg gcatacatca acatgtcttg aataattttc gatgttcgtt
     3721 cgatttgagc ctccaccgtt tctaagcgtt gtcgagactc cgcgctcagc gtcggatctt
     3781 cggccag
//
LOCUS       DATIPF010000338         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1393539, whole genome shotgun sequence.
ACCESSION   DATIPF010000338 DATIPF010000000
VERSION     DATIPF010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393539"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..739
                     /locus_tag="VLL06_08475"
     CDS             176..739
                     /locus_tag="VLL06_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31039.1"
                     /translation="MMMPTHMPVYVLLGGLILGSGTSLWAQVPGRANISSGPVGASMA
                     VLAMLQDADVLPPEDTPEANHVIKVVIQFQSVFMKSGDPTVQAFLSQALAAKAGSGAD
                     DIRSQFQSTGWTSDVLESLGEQWDAMAIDQRERLAPGFRQFNVSLADFGLLRNLVAKA
                     RTALEQRGQNLHQVFAQRRREMPGSAP"
     gene            759..995
                     /locus_tag="VLL06_08480"
     CDS             759..995
                     /locus_tag="VLL06_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSS31040.1"
                     /translation="MATKAYILIKVKAGQTKDVLQSVKRLTGVEQAHSCFGRPDIILF
                     ISVADERALSDMVITKIHQIGGVEETDTHIVADT"
     gene            complement(1285..2262)
                     /locus_tag="VLL06_08485"
     CDS             complement(1285..2262)
                     /locus_tag="VLL06_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSS31041.1"
                     /translation="MPLRIGLIGAGRHGSRYIHHLLQDLPGVSLAALCRKRIEESLPS
                     LPTTEIPVYGDYRDLIADPTVEAVVVVTPPTLSPEICLEAVKARKPLLIEKPLAPTGR
                     EARAMVAAAEEAGLLLMTAQTMRFESAILLLKDHLPEIGRLRYATFTSRIEMKPEAKV
                     WSTTAGQRGALLEFGIHLLDVASFLTGEEVVAVRCELDQIPAIAPDTMAIVQAQTTSG
                     IHCVMDVARVVAGRVARTEWVGTQGQISADWFHHRVTGVIGDTAPHEWVVRPQQTILA
                     TLRAFVRAIETNTPPPITGRDGCRAVEVADACYRSAELGGASVTVSSLR"
BASE COUNT          548 a          764 c          696 g          534 t
ORIGIN      
        1 attccaaatg agagcccgct gtcttcatac agaatcgtcc cgccgcgtat ccgcgttttc
       61 agcagcaccg tcatgcagga tgtgggctag caccctctcc tccccttcta tataatccct
      121 ccctatgctt gtgtcctgcc gcgtccgatc gaacctcctg gaacgtgcct cacacatgat
      181 gatgcccacc cacatgccgg tgtatgttct tctcggtgga ctgatcttgg ggtctggcac
      241 ttcgctctgg gcccaggtac ctggccgtgc gaacatttca tctggaccgg tcggagcctc
      301 gatggccgtc ctcgccatgt tgcaagatgc cgatgtgctt ccgcccgagg atactcctga
      361 ggccaatcac gtcattaaag tagtcattca atttcaatcg gtgtttatga agagcggtga
      421 tccgaccgtc caagcatttc tgagccaagc attggcggca aaggccggaa gcggtgcaga
      481 cgatatccgg tctcagttcc aatcgacagg ctggacatcg gatgtgcttg aatcactcgg
      541 tgaacagtgg gacgccatgg cgatcgatca acgagaacgg ttggcgcctg gctttcgcca
      601 attcaacgtg agtctggcgg attttggttt attgaggaac cttgttgcaa aggcacgcac
      661 cgccttggag caacgaggac agaacctgca tcaggtgttt gcgcaacggc ggcgagagat
      721 gccgggtagc gcgccataac tctaagaggg aggacgcaat ggcaacgaaa gcctacatcc
      781 taatcaaggt caaggccgga cagacaaaag atgtgctgca gtccgtcaag cgccttactg
      841 gcgtcgaaca agcgcactcc tgctttggtc ggcctgacat tattctcttc atcagtgtcg
      901 cggacgagcg agctctctcc gatatggtca tcacaaagat ccaccagatt ggaggagtgg
      961 aggaaaccga tacgcacatt gtcgccgata cctagaaata tgatcgagag taagaagtgt
     1021 ctatcatcgc gcgatgctca aaaagtcggc cttctcaccc gcccaaccct ggcggatgta
     1081 tcacccaccc gccctgagtc tgccaagaca gcctcttcgc cccgggatgc gccttgtccc
     1141 aggcaggcgg cagcaaacta tcccttttaa aggggtggct gggatgatcc caactgcgcg
     1201 cgtccaacga gggccttctg aggccgcgcg ttgcgcgagc acggggatcg tcctagctac
     1261 cctgtcccat ttttcagcat cctgctagcg gagggaagag acggtgacgc tggctccgcc
     1321 aagctctgcc gaacggtaac aggcatcagc cacctcgacg gcgcgacatc catcccgccc
     1381 cgtgataggc ggcggagtat tcgtctcgat ggcacggaca aaggcgcgga gcgtggcaag
     1441 gatggtctgc tgcgggcgaa cgacccattc atggggagcc gtgtcaccaa tgacacccgt
     1501 cacccgatgg tggaaccaat cagcggagat ctgtccctgg gttcccaccc actccgttct
     1561 tgcaaccctt cccgctacga ctcgtgcgac gtccatcacg caatgtatgc cgctcgtggt
     1621 ctgggcctgc acaatcgcca tcgtatccgg tgctatcgcc gggatttggt caagctcgca
     1681 gcggaccgcg accacctcct cgcctgtgag aaaggacgcc acatccaaga ggtgaatccc
     1741 aaattccaag agcgctccac gctgaccggc cgtggttgac caaaccttag cctcgggttt
     1801 catttcaatc cgactggtga aggtggcata tcggagccgg ccgatctccg gcaagtgatc
     1861 ctttaacagg agaatcgccg attcgaagcg catagtctgt gcagtcatca gcaagaggcc
     1921 tgcctcttcc gccgcggcta ccatcgctcg tgcttcgcgg cctgtcggcg ctaacggctt
     1981 ttcaatgagg agcggttttc tggccttcac tgcctcaaga cagatttcgg gactgagtgt
     2041 tggaggggtc accacgacca cggcctctac cgtcggatct gcaatcaaat cgcgatagtc
     2101 gccgtataca ggaatttctg tcgtcgggag cgacggcagc gattcctcga tccgtttccg
     2161 gcagagagcc gcaagactca cacctggcag atcttgcagg agatgatgga tatatcggct
     2221 cccatgccgg ccggcaccga tgagaccgat tctaagcggc ataggcgcca tatccctctg
     2281 tgaaggtgag aaactgtagt cgccattgaa cgcagggtca actacaagtt gagccccata
     2341 gatagcaaga ttgacagcct ggcggcccgg tccctatagt acgcgcgcct acaaaacctt
     2401 gatggaaaag agattgccgc aatgacatcg accatcgctc cctttacctt ggatcaaatc
     2461 gggaccggca ccgctcgttt tccgagtggt gtcgccattc ccaccctgac ggatgccgcc
     2521 gtcgatccct acattcagac ac
//
LOCUS       DATIPF010000339         4992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_333621, whole genome shotgun sequence.
ACCESSION   DATIPF010000339 DATIPF010000000
VERSION     DATIPF010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333621"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            361..933
                     /locus_tag="VLL06_08490"
     CDS             361..933
                     /locus_tag="VLL06_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31042.1"
                     /translation="MARVHLSHAQKLSAPGHALGSLRVVDQLLEESITYSQVLIEELT
                     PAALRTSGLMSALDMLAVELRQGGYQAHVPTSPVNLRLSEPITAFLYRAIRSFLRQAR
                     GEKVSSLITVSMEGDPAGIVRVRAAINGEARFPGVEEALLLGSSMEMNISGIQALGTL
                     LGIAISVESSPGTQSALVFDIPLQGSSTLL"
     gene            1309..1641
                     /locus_tag="VLL06_08495"
     CDS             1309..1641
                     /locus_tag="VLL06_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31043.1"
                     /translation="MAIPISRQKSNRDHNIDPIGPLDYILDLAHARNCSWIEKAAPGE
                     AIKVLVYPARDPLAPRVVGLGIRTSNGESLMVSFTTLDGERGAYSLDYQELLRTISLL
                     KTKPAEIN"
     gene            complement(1796..4549)
                     /locus_tag="VLL06_08500"
     CDS             complement(1796..4549)
                     /locus_tag="VLL06_08500"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HSS31044.1"
                     /translation="MRAKLTSLQFVGIVLVLAAVYVIAAKLGLMLAFVHASATAVWPP
                     TGITLAAFLILGYRVWPGIFLGAFLANLLTAGSMMTSLGIAMGNTLEGLIGVYLMNRF
                     ANGQHAFSSAQGVCKFAVLAGLVSTTISATFGVTSLAGGGFASWANYPSIWTTWWLGD
                     IAGNILVAPTFVLWAQHPQVRWNRDQALEATLSMVVLVGIGLALFTNVVPGTLKNYPL
                     VFLAVSSLVWVAARFSERDTVTATFLLSGIAIWGTLQGFGPFTRESPNESLRVLQAFL
                     GLIGIMVLALAASMSERKRAEAAVRDAHDALEQKVTERTSDLSAANRELEQEVAERRH
                     TEARLHERTDALRESEARVTTYAQELEQKNRDLDKALVEAQAATQAKSAFLAIMSHEI
                     RTPMNAIMGFNGLLLETPMTAEQRDYAETVQRSSEALLDIINDILDFSKFEAGGLTFE
                     TIDFDLRTTVEEALDLFAKPAQRKGLELGCLLHAEVPTALRGDPGRLRQILINLIGNA
                     LKFTQQGEVMIHVTRSQESADRTLIEFAVVDTGIGIAPEVQGLLFKPFTQADTSTTRK
                     FGGTGLGLAICKQLVERMGGQIGLESALGQGSTFRFTVWLTQQQTHATPIPRGSLAGR
                     RLCIVDDNASNRRILEQYAFQWGLHSATASDGDEALALLKEAAAEGEAFDLAILDLQM
                     PRMNGLELARTIKSDPLLTATRLVLLTSMGMRGQAEQAKQAGIAAYLSKPVHRSHLYD
                     CLTMIVDMSAKSSTDRLEVGTASRLHDVLVTRHVIKEAAMAARVRILVAEDNIVNQKI
                     AVGQLEKLGYRADVVANGLEAVDAVARIRYALVLMDCQMPEMDGWEAAAMIRKHEGAR
                     GNQRVPIIAITANAMPGDREKCRKAGMDDYLAKPVTLEELQVAVARWIPDCSTPAAQK
                     ELV"
BASE COUNT         1089 a         1428 c         1386 g         1089 t
ORIGIN      
        1 gagcaaggga accgaaatag cgcattggat atgtgaacat tgctaccaag aagggagaaa
       61 atctcttctc cacaattcgc agaagcatga ccacggaatt atttatatgg tcaaatgttt
      121 tcattgcaaa ttcgacacgg agatacaagg tgaaactgag cgccgatatg tatagagagg
      181 tagggctgat tgatggattt ggaaggactg aacagagggg cgaagaaacc aaaatagccc
      241 actacctagc gtccgaaatg aactggaaaa ttttgtctat gtgcgaagca cggatctcat
      301 gctcctatag cgcctgccat tgatggcttc gtcacgaggg atgaagacgg cttcctcgtc
      361 atggcacgag tacatttgtc ccacgcacag aaactttcgg cgccaggaca cgcattgggt
      421 agcctcagag tcgtggatca gttgttagaa gaatcgatca cgtatagcca ggtattgatt
      481 gaggagttga ctccggccgc cctgcgaaca agcggcctca tgagtgcgct cgacatgttg
      541 gccgtcgagc tacgccaggg tggatatcag gctcacgtgc cgaccagtcc ggtgaatctc
      601 agattgtccg aaccaatcac cgcttttctc taccgagcta tccgtagctt tctccgacag
      661 gccaggggtg agaaggtgtc ctcgctgatc accgtgtcga tggaaggcga cccggcaggg
      721 atcgtacgcg tgagggcggc gatcaacgga gaggctcggt tccccggagt cgaggaggca
      781 ctcctgcttg gatcgagcat ggaaatgaat atttccggaa ttcaggctct cggcactctt
      841 ttgggcattg ccataagtgt ggagtcttcg cctgggacac aatcggctct ggttttcgat
      901 attcctctgc aaggatcttc caccctcctc tgatttcacg ccctccttcg ggttggtctt
      961 cgagccttgg cgaaccttgc gactcgaacc aaaacggccc tagtcacagt gggaaaacgg
     1021 agtctgaccc tctttcctat tgctcgaact gtaattgagg accacggctg cgccttcggc
     1081 ggccaagtgc cgggcgaggg aagcgccgac gcctttggat gtgccccgtg gcgaccgcga
     1141 cgcctgtctc cttttctaca tggaggccat cgccttgtct cagttcacgc gctaaccgcg
     1201 cggataaact cagcccgatg gaccgtcgcg gtcgtcgggc tacaacatgc ctgcgcgttc
     1261 aagaagtttg cttgctattt aatcgaattg agatatagtt ccctacagat ggcgatccct
     1321 attagtaggc aaaaatcaaa tagagaccac aatatagacc ccattgggcc gctcgactac
     1381 attctcgatc ttgcccacgc gaggaactgt tcatggattg agaaggccgc accaggcgaa
     1441 gccattaagg tgctggttta tcccgcacgc gatcccttgg ctccacgagt cgtaggcctg
     1501 ggcatccgca cttcgaacgg tgaatccctc atggtgagtt tcaccacgct tgatggggaa
     1561 cgtggcgcct attccttgga ttaccaagag ctcctgcgaa ccatctctct cctcaagaca
     1621 aaaccggcag aaataaatta ggccggcgtt cgcacgccaa ggaaatcttc cgtcgagact
     1681 acgatacaca gccgtcattt ctttctttat cttaatcagc gggccctgct gctatcacat
     1741 cgagactgaa aagtgttcgc gcgggacaca ccgcagcgtg tgcttgtggg tccagttaca
     1801 ccagttcctt ctgagcagca ggcgtggagc agtctggaat ccatcgagcc acggcgacct
     1861 ggagctcttc gagcgttacg ggtttggcga gataatcgtc catgcctgcc ttccgacact
     1921 tctcacggtc ccctggcatg gcgttcgcgg tgattgcgat gatgggaact cgttggtttc
     1981 cccgtgcccc ttcgtgcttg cggatcattg ccgctgcctc ccatccatcc atttcgggca
     2041 tttggcagtc catcaagacc aacgcatacc tgattcgagc gaccgcgtcg accgcttcga
     2101 gcccgttggc cacgacatcg gcccggtagc cgagcttttc cagctgaccc accgcgattt
     2161 tctgattcac gatattatcc tctgccacga ggatgcggac gcgcgccgcc attgccgctt
     2221 ccttgatgac atggcgggtc accaacacgt catgaagccg actcgcggtc cccacctcca
     2281 gccgatcagt ggatgatttg gcagacatgt ccacaatcat agtcagacag tcgtataagt
     2341 gtgaacgatg tacgggcttg cttaggtagg cggcaatccc cgcctgtttc gcctgttcgg
     2401 cctgccctcg catccccatt gaggtcagca ggaccaggcg ggtggccgtc agcaggggat
     2461 cagatttgat cgtgcgcgcc aactccagtc cgttcatgcg aggcatctgc aaatcaagaa
     2521 tcgccaaatc aaaggcttcc ccctcagccg ccgcctcctt cagcagcgcg agggcttcat
     2581 ctccatccga ggcggtggcg ctatggaggc cccactggaa cgcgtattgt tccaggatgc
     2641 gtcggttgct ggcattatca tccacaatac ataaccgacg gcccgcaaga gaccctcgtg
     2701 gaattggtgt cgcgtgagtc tgttgttggg tcagccaaac tgtaaaccgg aaggtgctcc
     2761 cttgacccag agcactctct agtccgatct ggcctcccat ccgttccacg agctgtttgc
     2821 aaatggcaag gcccagtccc gtgccgccaa acttgcgcgt ggtcgaggtg tccgcctggg
     2881 taaacggctt gaacagaagc ccctgtacct cgggggcaat gccgatcccc gtgtcgacca
     2941 ccgcaaattc gatcagagtg cggtcagcgc tctcctggct ccgtgtgaca tgaatcatca
     3001 cttcgccctg ttgggtaaac ttgagcgcat tgccaataag attgatgagg atttgacgca
     3061 atcggccggg gtcgcctcgt aacgccgtgg gcacttccgc atgcaggaga cagcccaatt
     3121 ctaggccctt gcgctgcgcc ggcttggcaa agagatccag agcctcttcg acggttgtcc
     3181 ggaggtcaaa atcgatcgtc tcaaaagtca acccgcccgc ctcgaacttc gagaaatcaa
     3241 ggatgtcgtt gatgatgtcg agcagggctt cgctggaccg ctgcacagtc tcggcgtagt
     3301 ctcgctgctc ggcagtcatg ggtgtctcga gcaagagtcc gttaaacccc atgatggcgt
     3361 tcatgggagt acgaatttca tggctcatga tggcgaggaa ggcggacttg gcttgcgtgg
     3421 cggcctgcgc ctcgaccaac gccttgtcga ggtcacggtt tttctgctcc agttcttgtg
     3481 cataggtggt tactctggct tcgctctccc ggagcgcgtc cgttcgctcg tgcaatcgcg
     3541 cttcggtgtg ccgacgctcg gcgacttctt gttccaattc ccggttcgcc gcggagaggt
     3601 cgctcgttcg ctccgtcact ttctgttcca atgcgtcatg cgcgtcacgc actgctgcct
     3661 cggcccgttt tcgttctgac atactcgccg ccagcgccaa gaccatgatg ccaatgaggc
     3721 ccagaaacgc ttgcaatacc cggagggact cattcggcga ctccctggta aatggaccga
     3781 agccctgcag ggtgccccag atggcgatcc ctgaaagaag gaacgttgct gtgacggtgt
     3841 cccgttcact gaagcgggct gcgacccaga caaggctcga gacggccaga aacacgaggg
     3901 gataattctt gagggtccct ggaaccacat tggtgaataa tgccaggcct ataccgacga
     3961 gcactaccat cgagagagtc gcttcaagcg cctggtcacg attccatcgc acttgtggat
     4021 gttgcgccca caggacgaat gtgggagcca cgaggatgtt ccctgcgata tcgcccagcc
     4081 accacgtcgt ccaaatcgat gggtagttgg cccaactggc gaagccgcca ccagcgagac
     4141 tggtcactcc gaatgtggca ctaatggtgg tgctcaccaa tccggcgagc acggcgaact
     4201 tacacactcc ctgtgcagag gaaaacgcat gctgaccatt ggcaaatcgg ttcatcaaat
     4261 acacgccaat gagtccttca agcgtgttgc ccattgcgat accgagggac gtcatcatcg
     4321 agccagcagt aagtaggttg gccaggaacg caccgaggaa aatacctggc cagacacgat
     4381 atccgaggat caggaacgcg gccagcgtaa tgcctgtggg tggccacacc gctgttgcgc
     4441 tagcgtgaac aaacgccaac atcagtccga gtttggccgc gatgacatag acggcggcca
     4501 gaaccaggac gatcccaaca aactggagac tggtcagctt ggccctcata ggatctgact
     4561 cctcatgaca agatagaagg cgatctctcg ctccgaggag ttcgagcgtt ggttcggcag
     4621 gtgacccaca tgatccggac gggtaccatt aatagcgcgg gcatactcac tggggaggtc
     4681 aggagagaaa acagagtgtg gaaaccggcc ttgaagaaaa gcggattcat cggttgtgcc
     4741 cggaagccga gtcatcaccc aaggcgccag ccacgcaggg ccccatagac gtgacggggt
     4801 gcgctgttga ccttgtagac gcatgaatgc ctctcgggga cagaggtcta aagcgtaacg
     4861 tcccggttcc ttagtgagaa taccacttgt aaaagttttg tctggacatc ccgtgcactg
     4921 gcaatatccg ctcggatttc ttacgcaata accagtagcg tatggctgat gcgaggattg
     4981 cccccccgat tt
//
LOCUS       DATIPF010000340         6016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_861522, whole genome shotgun sequence.
ACCESSION   DATIPF010000340 DATIPF010000000
VERSION     DATIPF010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861522"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VLL06_08505"
     CDS             complement(<1..275)
                     /locus_tag="VLL06_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS31045.1"
                     /translation="MSSNAMQPIGQAADPSAWAKSPSATILLVEDELVARTSMAARLT
                     RLGYRVLEAENGRVALDVARRERPDLFIVDWMMPEMDGPTFCEAVRC"
     gene            complement(302..640)
                     /locus_tag="VLL06_08510"
     CDS             complement(302..640)
                     /locus_tag="VLL06_08510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSS31046.1"
                     /translation="MQISERTVPQGIILDLVGDLTYANRAVFKTAVEKAKQAGCRHLI
                     VNLEQVRFVDSAGLGLLVLVSQNLKLLQTKVSMLKPQRYVLEIMSLANIPKLIPIYEN
                     EEDALMGRAA"
     gene            complement(814..1863)
                     /locus_tag="VLL06_08515"
     CDS             complement(814..1863)
                     /locus_tag="VLL06_08515"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HSS31047.1"
                     /translation="MAKIRVLTVDDSALMRQVLATLLAKDPDIEVVGSAPDPYIAREK
                     IKALNPDVLTLDVEMPKMDGLTFLEKLMRGHPMPVVMVSSLTEAGCQTTLRALELGAV
                     DFIAKPKVDLREGMEEVAFDLIAKVKAAAKATVRPKQALLPGEVVSPKLLGSAMIKTT
                     DTIIVIGSSTGGTEAVKDILEVLPPSTPPILITQHMPERFTKTWAERLNNLSRISVKE
                     AEDGDSVLPGHALVAPGNYHMTLVRSGARYSVKISQGEPVNRHRPSVDVMFDSVAQYA
                     GANTVGVILTGMGGDGAKGMLRMKQAGAYTIAQDEASCVVFGMPKEAIKLGGVDKVLP
                     LAEIPAAMMAYVCKL"
     gene            complement(1941..2552)
                     /locus_tag="VLL06_08520"
     CDS             complement(1941..2552)
                     /locus_tag="VLL06_08520"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31048.1"
                     /translation="MTQIDTDQFTHIRRMRDNRFPYEIASILPGEFFVSRDPMIVYTV
                     LGSCISACIRDPVAGVGGMNHFMLPEPKEKAHDSWGESTRYGSYAMESLINEILKRGG
                     LKRRLEVKLFGAGKIYESNIDIGARNAEWVVTFLRMEGLVPTKTDLGDVFPRKVYYFT
                     DSGRVLMKKIERIKNQTIFARESAYAAKIQSVKQQPEEDVTLF"
     gene            complement(2558..3376)
                     /locus_tag="VLL06_08525"
     CDS             complement(2558..3376)
                     /locus_tag="VLL06_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase"
                     /protein_id="HSS31049.1"
                     /translation="MDYGISTDEFERFRTLIYDESGISLSEQKRTLLASRLSKRLRDL
                     ELATFSDYYAKVTEDPTREEFTRMLDLISTNKTDFFREPKHFAFLRERILPELERTKR
                     IRIWSSACSSGEEPYTIAMTLFDTVRNPAEWDFKILASDLSTRVLAKAATGTYDEERV
                     RDIPPDVIRRHFLRGRGESAGLLKVKPHLAEMIQFRRLNLMDDRFPIKSALDLIFCRN
                     VMIYFDRPTQETLVNKFHHYLKPGGYLFIGHSESLQWVRQPFKSLAPTIYQKES"
     gene            3613..4005
                     /locus_tag="VLL06_08530"
     CDS             3613..4005
                     /locus_tag="VLL06_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS31050.1"
                     /translation="MTPRFDQNLYRYRVDLVANGLEVLNALTDIRYAAVLMDCQMPEM
                     DGFEATAEIRRKEGANDHLPIIAMTANAMAEDRDRCLAAGMDDYMSKPVQSSVLAEVL
                     SRWVSAPASYPRITGNSPLQAASVKRDL"
     gene            complement(4571..>6016)
                     /locus_tag="VLL06_08535"
     CDS             complement(4571..>6016)
                     /locus_tag="VLL06_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HSS31051.1"
                     /translation="YTHLKGALGYPGMNWSVLMAVDQVLAAADARAINRNVLIAIIVC
                     LVILLALGILIGRIGVRGISLVSQAAAKLAGGALDTRVSVTSKDEVGQLAQAFNQMGE
                     QLQAKVAAERAHAVKMEQFMGEAKRVLGAMAQGDLTDQMTQAAEGDLEQIKRSINEVV
                     ENLTQTISVVRSGVEHVSSGAEQITKGNEDLSQRTSEQASSLEETSSAMEEMTSTVKQ
                     NADNAKQANQLAIAARDVANKGGAVTTKAVEAMGAINQSSKKIADIITVIDEIAFQTN
                     LLALNAAVEAARAGEHGRGFAVVAAEVRNLAQRSATAAKEIKGLINESIQRVTDGSEL
                     VDQSGKTLAEIVSSVKRVTDIIAEITAASQEQASGIDQVNKAIMQMDETTQQNAALVE
                     ETTSASQSMKEQAKELIRSVEVFKTVQAKGHLTTHVGPRETQPATAIPTARVHEPKPM
                     DKKPTEKHEPVGVAASNGKDCSNTNDGFEEF"
BASE COUNT         1208 a         1848 c         1574 g         1386 t
ORIGIN      
        1 tcacagcgca ccgcttcaca gaacgtcggg ccgtccatct ccggcatcat ccaatcgaca
       61 atgaacagat ccggacgctc tcgccgggcc acgtcaaggg cgacccgtcc attctcggcc
      121 tccaggactc ggtagcccaa ccgagtcaac cgtgcggcca tactggtccg cgcgaccaat
      181 tcatcctcca ccaggaggat ggtcgcactg ggcgacttag cccatgcaga cgggtcggca
      241 gcctgtccaa tgggttgcat ggcgtttgaa ctcatcacac tccgtgactg acacaccgag
      301 cctatgctgc acgccccatg agcgcgtctt cttcattttc atagatcggg atgagcttgg
      361 gaatattcgc caggctcatg atctccaaca cgtagcgctg cggtttcagc atgctgacct
      421 tggtctgcag cagtttgagg ttctgcgaca ccagcacgag cagcccaagc cctgcgctat
      481 ccacgaagcg cacctgctcc aaattgacaa tgaggtgtcg gcagcccgcc tgtttcgcct
      541 tctcgaccgc cgtcttaaag acggcccgat tggcataggt caagtctcca accaggtcca
      601 agattattcc ttgtggcacg gtacgttcac tgatctgcat ggcgtcatcc tttcttgtgg
      661 ctattgacag aaggtctctc ttcttcgact gacccaaggc caggtttctc tggtctattt
      721 ggattgtgtt gttcagctgg ttggtctagt tcaactctcc aacgagaaaa accaaataaa
      781 ccagacaaac caaacaaacc cattaccggt cttctagagt ttgcacacat aggccatcat
      841 cgctgcggga atctcagcca gcggcaagac cttatccacc ccaccgagct tgatcgcctc
      901 cttcggcata ccgaacacca cgcaactggc ttcatcctgg gcaatcgtgt aggcaccggc
      961 ctgtttcatt ctcaacatcc ccttcgcgcc gtcgccgccc attcctgtaa gaatcacacc
     1021 gaccgtattg gctccggcat actgcgccac agaatcgaac atgacgtcga cggaagggcg
     1081 gtgccgattg accggctcac cctgagagat cttcaccgaa taccgagcgc cactccgcac
     1141 gagtgtcatg tgataattac ctggcgcaac cagcgcatgg cctggcagga cgctgtcccc
     1201 gtcttccgcc tctttgacgg agattctgga gagattgttc aaccgctccg cccacgtctt
     1261 ggtgaatcgc tccggcatat gctgggtgat gagaatcggc ggcgtgctcg gcggcagcac
     1321 ttccagtata tccttcacgg cttcggtccc accggtagag gaaccaatga caatgatcgt
     1381 atcggttgtc ttgatcatcg ccgacccgag cagcttcggc gatacgacct cgccaggtaa
     1441 gagggcctgc ttcggacgca cggtggcctt tgccgcagct ttgaccttgg cgatgagatc
     1501 aaaggcgact tcttccatgc cttcacgcag gtcgaccttc ggtttggcga taaaatccac
     1561 ggctccgagt tccaacgccc gtaaggtcgt ctggcatccg gcttccgtga gcgaactcac
     1621 catcacgacc ggcatcggat gcccccgcat gagcttctcc aggaaggtca gtccgtccat
     1681 cttcggcatt tcgacatcga gcgtgagcac gtcgggattc aacgccttga tcttctcccg
     1741 ggcaatgtac ggatcgggag cggacccgac cacctcgatg tcgggatcct ttgccagcag
     1801 tgtggccagc acttgccgca tcagagccga atcgtcgacg gtcaacacac gaatctttgc
     1861 catagtccct ctcgttactg agtttcaggt ttcttgtttc aggttcctgg ttcaacgtga
     1921 aacatgaaac ttcttcaccg ctagaacagc gtcacatcct cttccggttg ctgtttcaca
     1981 ctctgaatct ttgccgcgta tgccgactca cgggcaaaga tcgtctggtt cttgatccgt
     2041 tcgattttct tcatgagtac acgtcccgag tcggtgaagt aatacacttt cctgggaaac
     2101 acatcgccga gatccgtctt ggtgggcacc aacccttcca tcctgaggaa cgtcacgacc
     2161 cattcggcat ttctggctcc gatatcgatg ttgctctcat aaatctttcc ggcaccaaac
     2221 agcttgacct cgaggcgcct cttcagtccg cctcgcttca gaatttcatt gatcagggac
     2281 tccatggcat acgacccata ccgggtggat tctccccagg agtcgtgggc cttttctttc
     2341 ggttccggca gcataaagtg attcatacct ccgactccgg ccaccggatc gcgaatgcag
     2401 gccgagatgc aggagcccaa caccgtgtag acgatcattg gatcacggct cacaaaaaac
     2461 tctcctggta gaatcgacgc gatctcatag ggaaaacgat tgtctcgcat acgccggata
     2521 tgcgtgaact ggtccgtgtc aatctgtgtc atgcgcctca ggactccttc tgataaatcg
     2581 tcggggccag cgatttgaag ggttggcgga cccattggag gctttcggaa tgcccgatga
     2641 acaggtagcc ccctggcttc aggtagtgat ggaacttatt gacgagcgtt tcctgtgtcg
     2701 ggcgatcgaa atagatcatg acattccggc agaaaatgag gtcgagggca ctcttgatgg
     2761 gaaaacgatc gtccatcaga ttgagccgtc ggaattgaat catctcggcc agatgtggct
     2821 tgaccttgag cagaccagca ctctcgcctc gcccccgcag gaaatgtcgt ctaatgacgt
     2881 caggcggaat gtcgcggacc cgctcctcgt cgtacgttcc cgtggctgcc ttcgccaata
     2941 cgcgggtcga gagatcggag gccagaattt tgaaatccca ttcggcggga ttccggacag
     3001 tatcaaagag agtcatggca atcgtatagg gctcttcccc gctcgaacag gcggaagacc
     3061 agattcgaat gcgtttggtg cgttccagtt caggaaggat ccgttcacgc agaaaggcga
     3121 agtgtttggg ttcgcggaaa aagtcggtct tgttcgtcga gatcagatcc agcatccgcg
     3181 taaactcttc gcgcgtcgga tcttccgtca ccttcgcgta atagtccgag aaagtcgcca
     3241 gttccagatc gcgcagccgc ttcgacaggc gggacgccag caacgtcctc ttctgctcgc
     3301 tcagagaaat gccgctttca tcatagatca gcgtacgaaa ccgctcgaac tcatcggttg
     3361 agatcccgta gtccatggtg cacatagctc cgctggcaaa tgacgtggca tccccttcct
     3421 ccaccggaat ccggggtatt cctctatcgg tcggttgtta tcgattctta accactggaa
     3481 tacccggaac agggttcggt ctgacgagaa accatcgaca gagcatcaga tctacgcgcg
     3541 cttccaggta gccgatggct tgcgaacctc ccgactcgtg atcggccatc attaagggat
     3601 ccgtaaaatc aggtgactcc tagatttgac cagaatctgt acaggtatcg ggtggatctc
     3661 gtggccaacg gcctggaggt tctcaacgcc ctgacggaca tccgctatgc cgcggtctta
     3721 atggattgcc agatgcctga aatggacggg ttcgaagcca ctgcagaaat tcgccggaag
     3781 gaaggggcga acgaccatct tccgatcatc gccatgacgg caaatgcgat ggccgaagac
     3841 agggaccgtt gtctcgccgc cggcatggac gactacatga gtaagcctgt gcaatcttcg
     3901 gtcttagcgg aggtgctctc gcgctgggtc agcgcgcctg cctcctaccc tcgcataacc
     3961 gggaacagtc ctttgcaggc tgcgtctgtc aagagagacc tgtaaccatc acagagtcag
     4021 ggaacaaggg cctcgagaaa actgttctat cactccgtat ctctggcgac ccaccatcgc
     4081 actacccaac gccgcgcgag cgcgacagac gaacgctggc gaagactttt catcgataat
     4141 aacggaagac cttgtctaat cggcaaccca ggcctggctc gtgtgaggta agtaggttct
     4201 ggctgaaggc tcacgttgca gagggagtat gcgccactcg tcgtgcgtcc gttcagtaca
     4261 tgcgatctga atcaggctac gtcacttatc gcgctacgaa tcacgcgcgt gaataggtgc
     4321 actccagcaa attgacttag gaggcatgag aacgtttacg gtcggcgtcg agccaatcgt
     4381 ccacatcttg tcgtgtgtcg atgtcctctg ggcatggagt ccgtatgcgc gctgggcaat
     4441 cttctaccgt tgccactcag ccaagaatgc tgccgggatc gatgcgggct catgtagcac
     4501 agtgtggcac tgggtgactt tcgtatctct ccccaagcga catcacgtgt cgctcatggc
     4561 acgcatccgg ctagaattcc tcgaacccat cgttcgtatt gctgcaatcc ttgccattgg
     4621 atgcggcgac accaaccggt tcatgcttct cggtcggttt cttgtccatc ggtttcggct
     4681 catgaacgcg agccgtcggt attgccgtgg caggctgggt ctcgcgcggg ccgacatgag
     4741 tggtgaggtg gcctttcgcc tgaacggtct tgaagacttc cacgctccgt atcagttcct
     4801 tcgcctgctc cttcatggat tgactggcgc tcgtcgtctc ttccacgagc gcggcgttct
     4861 gctgcgtcgt ctcgtccatc tgcataatcg ccttattcac ctgatcgatc ccgctggcct
     4921 gctcctgtga cgccgctgtg atctcggcaa taatatccgt gacccgcttc accgagctca
     4981 caatctccgc cagcgtttta ccggattgat ccaccagctc gctcccgtcc gtcacccgct
     5041 gaatggactc gttgattaac cccttgatct ccttggccgc cgtcgccgac cgctgcgcca
     5101 ggttccgcac ctcggccgcc accaccgcaa acccgcgccc gtgttccccc gcgcgcgccg
     5161 cctccaccgc cgcattcaag gccaagagat tcgtttggaa cgcgatctca tcgatgacgg
     5221 tgatgatgtc ggcgatcttc ttgctgctct ggttgatcgc ccccatcgcc tcgacggcct
     5281 tggtagtgac cgcgcccccc ttgttggcca cgtcgcgagc agcaattgcc agctggttcg
     5341 cctgcttggc attgtcggcg ttctgcttca ccgtggaggt catctcttcc attgcgctgg
     5401 atgtttcttc cagcgaggag gcctgttcac tggtccgctg cgagagatcc tcgttgccct
     5461 tggtaatctg ctcggcgccg ctcgagacat gctcgacccc gctgcgcaca acagagatcg
     5521 tctgggtgag gttctctacc acttcattga tgctgcgctt aatttgctca agatctccct
     5581 cggcggcttg tgtcatctgg tccgtcagat ctccttgggc catggcgccg agcacgcgct
     5641 tggcttcgcc catgaactgc tccatcttga ctgcgtgtgc tcgttctgcc gccaccttcg
     5701 cctgcaattg ctctcccatc tgattgaacg cctgggccaa ctgcccgacc tcgtccttcg
     5761 aggttaccga gacacgagtg tcgagggcac ctccggccaa cttcgccgcc gcctgactga
     5821 ccaggctgat tcctcgtacc ccgatccgtc cgatgaggat cccgagcgcc aggagaatca
     5881 ccaaacacac gatgatcgcg atcagcacgt ttcggttgat cgcccgggca tcggcggccg
     5941 caaggacttg atcgaccgcc atgagtactg accaattcat gcccggatac cctaacgccc
     6001 ctttaagatg ggtata
//
LOCUS       DATIPF010000341         3911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_509396, whole genome shotgun sequence.
ACCESSION   DATIPF010000341 DATIPF010000000
VERSION     DATIPF010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509396"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..169)
                     /locus_tag="VLL06_08540"
     tRNA            complement(92..169)
                     /locus_tag="VLL06_08540"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(131..133),aa:Glu,seq:ctc)
     gene            401..937
                     /locus_tag="VLL06_08545"
     CDS             401..937
                     /locus_tag="VLL06_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31052.1"
                     /translation="MNNPLQSNTIGITDSPELIDIEKPEQGLELHVGSNVFRNTNGVV
                     KLQGKEQLVLETQPDPLLLLLTMDLYDEQGTRIGHIRRNRLSVHSAGRFTINVNARSE
                     TVQDLSPSVTVADRTTGHTVFEVWLIQGRKIRITVGNFYTHKGELMTVSPHYCRIGTV
                     LTLFGNVAESRGNAAAIG"
     gene            1080..1493
                     /locus_tag="VLL06_08550"
     CDS             1080..1493
                     /locus_tag="VLL06_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31053.1"
                     /translation="MSDVAKPSVQAFLVCDQVIEDSVTKKKSLIGLFTHLQAVSFPFQ
                     HSQMGLYFCLTDAEGTYHFDIDLIYLNNEQLVCRAALPNIAIGDRLQISDFGINIPSL
                     VFPAPGRYEFRLRMEGHLIAQKDFSVMQMPTHQTA"
     gene            1622..3316
                     /locus_tag="VLL06_08555"
     CDS             1622..3316
                     /locus_tag="VLL06_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HSS31054.1"
                     /translation="MSELSGASNFIRDIVAADHAAGKHGGRVVTRFPPEPNGYLHIGH
                     AKSICLNFGIANNTPGGICHLRFDDTNPSTEDPEYVQAIQDDIRWLGFDWHERMFYAS
                     DYFERLYEFAVQLIRKRLAYVDTLTAEEMRVYRGTLTQPGQNSPFRDRSIDENLHLLQ
                     GMRDGKFPDGTHVLRAKIDMASANINLRDPVLYRIRHAAHYRTGNTWCLYPAYDFAHP
                     LSDALEGITHSLCTLEFEDHRPLYDWVVEQAEAPHRPRQIEFARLNLTYTVMSKRMLL
                     ELVEKKLVNGWDDPRLPTIKGLRRRGYTPEAIRAFCDHIGIAKRDAIVEMQLLEHFIR
                     EDLNKRAPRVMAVLRPLKIILDNYPEDQAEELEAVNNPEDPSAGTRKVPFSRVLYIEQ
                     DDFREDPPKQFFRLAPGREVRLRYAYIIKCVGVTRDPRTGEVTELHCTYDPTTKSGAS
                     QEQRKVKSTIHWVSAAHAVRAEARLYNPLLVTELAKIPPDHDWTTYLNPHSLERITNC
                     LVEPSLRQTIPGARYQFERLGYFCADPDSASGTLIFNRTVSLKDAWAKIEKTQPAR"
     gene            3527..3844
                     /locus_tag="VLL06_08560"
     CDS             3527..3844
                     /locus_tag="VLL06_08560"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS31055.1"
                     /translation="MIECREYPRIPVDMQIFFSSTNKTDIREGTMFDLSAGGCAVTSM
                     SSVQLGSAVRILIRATDLGSPITIESAAVRWSEGGEFGVEFLGLSEIDQRRLHRLLQV
                     ATT"
BASE COUNT          955 a         1138 c          957 g          861 t
ORIGIN      
        1 tttgtcctat ggattgtcct atggacgatc tgccgaggat acgactgtta tcgtgaaggc
       61 ggctaactca cggagagatg aaggaaaagt ttggtggtcc caacgggatt tgaacccgtg
      121 tttaagcctt gagagggctc cgtcctaggc caggctagac gatgggacca gctatcgagg
      181 atgtgggatc gacctggcac agtagcacag cggcttttca tttatcaatt cctaccaatt
      241 ccgtaccgta tatttcggcc acggcaccgt tgattcaatc acgacttgac agagaccgtg
      301 gtatagccta ccttgcggaa aatatgtgcg gcgggcccct aggagaggga attcttaggt
      361 tgtcgagctc ctcttcccat cagcaaactc atggtggaac atgaacaacc cgcttcaatc
      421 caatacaatc ggcatcacag actctcctga actgatcgac atcgagaaac ctgaacaagg
      481 actcgaatta cacgtcggct caaacgtctt tcgaaacaca aacggcgtcg tcaagcttca
      541 aggcaaggaa cagttggtgt tggaaacgca acccgacccc ttgttattgc tcctgacgat
      601 ggatctgtat gatgaacagg gaacccgtat tggtcatatc agacggaata ggctttcggt
      661 gcatagtgcg ggccgcttca ccatcaatgt aaatgcccgc agtgaaacag tccaagacct
      721 ctctccttca gtcaccgttg cagaccgaac aactggacac acggtgttcg aagtctggct
      781 catacaggga agaaaaattc gcatcaccgt gggaaacttc tatacccaca agggcgaact
      841 catgactgtt agcccacatt attgccgcat cggcaccgtg ctcaccctat tcggtaatgt
      901 cgcggagagc cgcggcaacg cagcggcgat tggatagtcg cttcccgata gaccacactc
      961 tcagtcctta tgtgtccact cccaacaatc aagatcgcaa caaccagcgc cgttagctgg
     1021 ctccttgttg aaaagcacat cctttttgac aaggcactgg actcccgtac aatggcagta
     1081 tgtcggacgt cgcgaaaccc agcgtgcagg ccttcttggt ctgcgaccag gtcattgagg
     1141 acagcgtcac caagaagaaa agcctcattg gcctcttcac gcacctacag gccgtgagct
     1201 ttcccttcca gcattcacag atggggctct atttctgtct caccgatgcc gaggggacct
     1261 atcacttcga catcgatttg atctacctca acaacgaaca gcttgtctgc cgagccgctc
     1321 tcccaaatat tgctatcggc gaccgccttc aaatttccga tttcgggatc aatatcccct
     1381 cgctggtctt ccccgctcct gggcgctatg aattccgcct tcggatggaa gggcatctga
     1441 tcgcgcaaaa ggacttcagt gtgatgcaga tgccgactca tcaaaccgcg tagcacatca
     1501 cgtcgctccc acatccttcc cattctgaga ggtcgataag gacttctccc ctgtgccgtg
     1561 cgtatggtag aacagcctgc aacgatctgg ttgcgaatct cccgggaatc aatgacatcg
     1621 catgtctgaa ctgtctggtg cttcaaactt tatccgcgac atcgtcgccg ccgatcatgc
     1681 agccggcaaa catggcggcc gagtggtcac gcgttttcca cctgagccaa acggctatct
     1741 ccatatcggc catgcgaaat cgatctgcct gaatttcggt atagcgaata acacccctgg
     1801 cggcatctgc cacctgcgat tcgacgatac caacccaagc accgaagatc ctgaatatgt
     1861 ccaggctatt caggacgaca tccgatggtt ggggttcgac tggcatgaga gaatgttcta
     1921 cgcctctgat tacttcgaac gactctacga gtttgccgtg cagctgatcc gaaaacgtct
     1981 cgcgtacgtc gacacgttga cggccgaaga gatgcgtgtc tatcgcggca cactgactca
     2041 gcctgggcag aacagtccct ttcgcgaccg ttccatcgat gaaaatcttc acctccttca
     2101 aggcatgcga gacggaaaat tccccgatgg aacccacgtc ctccgagcca agatcgacat
     2161 ggcatcagcc aatatcaatc tacgcgaccc ggtcctctat cgcatcagac atgcagcgca
     2221 ttatcgaaca gggaacacat ggtgtctgta cccagcctac gactttgcgc atcccctctc
     2281 agacgcgctt gagggaatca cgcactcgct ctgcaccttg gaatttgaag atcaccggcc
     2341 actctacgat tgggtcgtcg aacaggctga agcaccgcac cgaccacggc aaatcgaatt
     2401 tgctcgtttg aacctgacct ataccgtcat gagcaaacgg atgctgctcg agttggtcga
     2461 aaagaaattg gtgaacggtt gggatgaccc acgactgccg acgatcaaag ggcttcgccg
     2521 ccgagggtat accccagagg ccattcgtgc attctgcgac catatcggca tagccaagcg
     2581 cgacgcgatt gtcgagatgc agctgctcga acactttatt cgcgaagact tgaacaaacg
     2641 agcaccccgg gttatggccg tgctgcggcc actcaaaatc atcctcgaca actatcccga
     2701 agaccaggct gaagaattgg aagcggtcaa caatccggag gatccgtcgg caggaacgag
     2761 aaaggttccc ttttcgcgcg tcctctatat tgagcaagac gatttccgtg aagatccccc
     2821 caaacaattt ttccggctcg ccccaggacg cgaagtacgg ctccggtacg cttatattat
     2881 caagtgtgtc ggagtgacaa gagatccgag aacgggcgag gtcaccgaac tccattgcac
     2941 ctatgacccc acgacaaaaa gcggggcttc acaggaacag cgcaaggtca agtccaccat
     3001 ccattgggtc tccgcagccc atgctgtgag agcggaagcc cgactctata accctctctt
     3061 ggtgacagaa ctggcgaaga taccacctga ccacgattgg acgacctatc tcaatcccca
     3121 ttcattggag cgaatcacaa actgcctggt tgagcccagc ttgcgacaga cgatacccgg
     3181 cgcacgatac caattcgagc gcttgggata cttctgtgca gacccagatt cggcttccgg
     3241 cacactgata tttaaccgca ccgtgtcact caaggacgcc tgggccaaga tcgaaaaaac
     3301 tcagccggcc cggtaacgga ctgacaatct tctcctgttc gccagaacgc tctcagaact
     3361 tccctctttt ttcgacctgt tggaccccct ttacagaccc agcatccctg ctactcttga
     3421 gataactaag ttccctcatg gtgatatggc aaaacactgc ggtcctaact ggcgggagca
     3481 tgtggtgtcg gtaaaacgag aaacctcgaa ccggatgggt gattgaatga ttgagtgccg
     3541 agaatatccg cgcatccccg ttgacatgca gatctttttc tccagcacca acaagacaga
     3601 tatccgtgaa ggcaccatgt tcgatctctc tgccggagga tgcgctgtca ccagcatgtc
     3661 gtcagttcag ctcggttcag cagtgagaat cctcatccgg gcaacagatc tgggttcgcc
     3721 cattaccatc gagtccgccg cggtccgttg gagcgaaggc ggtgagttcg gcgtcgaatt
     3781 ccttggcctc tccgagatcg atcagcgccg tcttcaccga ttactgcaag tcgccaccac
     3841 ctaatacatc cgtccgagtt gatcgctgac cgatctacgg attcagcatg gtacaatgcc
     3901 gagcatgatg t
//
LOCUS       DATIPF010000342         3426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1295669, whole genome shotgun sequence.
ACCESSION   DATIPF010000342 DATIPF010000000
VERSION     DATIPF010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295669"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..1357
                     /locus_tag="VLL06_08565"
     CDS             371..1357
                     /locus_tag="VLL06_08565"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HSS31056.1"
                     /translation="MSQPLVRGRPSSLILLGMMMLVTGCTGDGGTAGGGAKACQTNCP
                     TAKAGPEQAVVTGTAVTLDGSGSTSGTPGLITYQWALTSKPNGSAATLVGAMTARPTF
                     TADVAGTYDARLIVQEGGASSAPDTVRITCGPGNLAPMADAGPDRTELLGTPVTLDGT
                     GNRDPNGTPITYAWRVVTQPAGSQAVLLNATSKTPSFTPQVAGPYTLALTVSDGALMS
                     PADEVVITVTADNCPPIANAGPDQQVATGQLVTLTGTGSTDPNHDPLTFSWRFQSKPD
                     GSTATVAAATSVSPIFRPDLAGLYVLSLVVSDGMRTSAHDTVVVEAGLPRFN"
     gene            1394..1705
                     /locus_tag="VLL06_08570"
     CDS             1394..1705
                     /locus_tag="VLL06_08570"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HSS31057.1"
                     /translation="MAQDGTGDLYVGGEFTAYKGTVGNQLMRLHPDGTVAQTFGQGFD
                     NLVGGIFLAKTGGGELYVLGGFTQLDGQPVRRLIPLTRMGSLEAGFRVPAELSSIVSG
                     P"
     gene            1793..2596
                     /locus_tag="VLL06_08575"
     CDS             1793..2596
                     /locus_tag="VLL06_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31058.1"
                     /translation="MKHMVLLRFLSSFLLMATIHGCTQKTIQVTSSHSSPCATINPTV
                     QSKSSVELALLFGTDAIKAAMDTIKRQIAMLDSTSTAQLINLGTDAAVQRAQASGKHL
                     IPEDRTMMETYLRDEMVPAIRQNPTCFTGSSSSMSHFGVERIFIAPSQALEIVITNSG
                     QDEAEAEIFIRQFVNGSERARSQGSLTLGPGLSRSVFATSAHLPISEVLNGTAMLMLV
                     VSISYHPEPNGKRVELNEAWQYDISYNRFILVPMNQYGTTLLPKQGKPS"
     gene            complement(2788..>3426)
                     /locus_tag="VLL06_08580"
     CDS             complement(2788..>3426)
                     /locus_tag="VLL06_08580"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HSS31059.1"
                     /translation="VDPAFSTGNSFPSTSSMGCPQTIEALMPTSSGKLYVGGTLNVYN
                     GVSVSSLLRLNQDGTLDSTFMSGFGAICNIPTVGDLAPAGDGTGDLYATLWVGPLYRL
                     HDTGAGVANFKTNTSNARGSRIAVTQDGSGDVLLSADIRLFRYNSSGELLTAPTFIPP
                     TMEGGAFTIVPIPDDTGDVYIGGTVTTYNGAAVNHFARIHADGSLASIVSSP"
BASE COUNT          760 a          977 c          941 g          748 t
ORIGIN      
        1 aattcaaaac cagttgtttt tgactccgct ggctcctggt cttgatcgtc aaggaaatca
       61 cgagcatacc tgctaggaat ttcacagaac tttcggacac ctcagtgact ccttacatgc
      121 ccagcggtct tttcttttgc gggaatggtt ctctaccgcc ctgtttacaa ggtttttccc
      181 ctccagactc gggttcttcc cagtctcgct ccctgagggc gtcagatagg aatatcccta
      241 gaacgggcaa ttacctaggc ctatcgcctg tcctttcccc attcgtcgca gtgcaccgga
      301 accgtccatc ctatgccgtc catcctttga caggctacca gaacggcgcg tccctgaaag
      361 gagcgatctc atgagccagc cattagtgcg cggtcgaccc agcagcctga ttctgctcgg
      421 gatgatgatg ctcgtcactg ggtgcaccgg cgatggaggc accgcgggcg gcggggcgaa
      481 ggcctgccaa accaactgcc cgaccgcgaa ggccggacca gaacaagccg tggtcacggg
      541 aactgcggtc accctcgatg gcagtggcag tacttccggc acacccggcc tcatcaccta
      601 tcaatgggcc ttgacgtcga aaccaaacgg cagcgccgcg acactcgtcg gggccatgac
      661 cgcgcggccg acctttaccg ccgatgtcgc gggcacctat gatgctcggc tcatagtgca
      721 agagggcggc gcgtcgagtg cgcccgatac ggtcaggatt acgtgtggcc ccgggaatct
      781 tgcgcccatg gcggacgcgg ggccggaccg gaccgaattg ctcggcaccc cggtcacgtt
      841 ggatgggacg ggcaatcggg atccgaacgg cacgccgatc acctatgcct ggcgtgtcgt
      901 gacgcaaccg gccggcagtc aggcggtctt gctgaatgcc accagtaaga cgccctcgtt
      961 tacgccccag gtggccggcc cttacacact tgctctcacg gtcagcgatg gcgcactcat
     1021 gagtcccgcc gatgaggtgg tgatcacggt caccgccgat aattgtcccc caatcgcgaa
     1081 tgctgggccc gatcaacagg tcgcgacagg acaattggtg acactcaccg ggaccggcag
     1141 cacggatccc aatcacgatc ccttgacctt cagttggcgg ttccaaagca aacccgacgg
     1201 cagcaccgcg accgtagcgg cggcgacctc cgtcagtccg attttcaggc cagacctcgc
     1261 cggattgtat gtgctgtcgc tggtcgtgag tgacggcatg cgcaccagcg cgcacgatac
     1321 ggtggtggtt gaggcgggac ttcccaggtt caactaaggt tctggattca acgatcgcgt
     1381 cacaagcatc gttttggctc aggacggaac cggggatctc tatgttggtg gcgaattcac
     1441 ggcgtacaaa ggaacggtgg gcaatcagtt aatgcggtta catccggatg ggaccgtggc
     1501 gcagacgttc ggtcagggtt ttgataatct tgtcggtggc atcttccttg caaaaaccgg
     1561 cggcggggaa ctctatgtgc tgggagggtt cacccaatta gatggacaac cagtgcggcg
     1621 tctgatcccc ctaactcgaa tgggttcgct cgaagcaggg ttccgggtac ccgccgagct
     1681 ctcgagtatc gtcagcggtc cgtagtgact tacgccagat tgaggcgtgt cctcaccgta
     1741 tgggactcgc catcaggaga gattcatagc aacacgccga tggtcacggc aaatgaaaca
     1801 tatggtcttg ttacgttttc tatcttcctt tctgctcatg gcgaccattc atggctgtac
     1861 ccaaaaaacg atacaagtga ctagctctca tagctccccc tgtgccacca taaatccaac
     1921 tgtccagtcc aagtcgagtg tcgaactggc actcttattt ggtactgatg ctattaaagc
     1981 ggcgatggat accatcaagc gacagattgc catgttagat agtacctcca ccgctcagct
     2041 cataaattta ggaacagacg ccgccgttca aagggcacaa gccagtggca aacatctgat
     2101 accagaagat aggaccatga tggaaacata tttgcgagac gagatggttc cggcaataag
     2161 acagaaccca acctgtttca ctggttcgtc ttcatcgatg tcacatttcg gtgtggaacg
     2221 gatttttatc gctcctagcc aagcattaga gatcgtgatc acaaattctg gacaggatga
     2281 ggcggaggct gaaatattca ttcgtcaatt tgtcaacggc tcagagcgtg ccaggtctca
     2341 aggctcactc actctaggcc ccggcctctc acgcagtgtg tttgcaacga gtgcccatct
     2401 tcccatttct gaagtgttaa atggcacggc tatgctgatg ctggttgtat ccatttccta
     2461 ccatcccgaa ccgaacggta agagggtgga gttaaacgaa gcgtggcaat acgacatttc
     2521 atacaataga tttattttgg ttcccatgaa ccagtatggg accactctac taccgaaaca
     2581 gggcaagcca tcctgatcct gcatgagaat ggttcctcga ccgtaacgtg gctggattgg
     2641 ccgccgggtc cagggtacct tgaggaaatg ggccatcccc cgcaacggcg agtccgtggc
     2701 cacggtatgc ttggtcacca aagaaatccc agccgtcagg attggatcct tcacataggc
     2761 ccgctgcctc aatctggctg acgtcactta tggactgctc acgatactcg ccagtgagcc
     2821 gtcggcatgg atccgtgcaa aatggttgac ggcagccccg ttataagtgg tgacggttcc
     2881 tccaatatag acatctcccg tgtcatccgg gatggggacg atggtgaagg ctcctccctc
     2941 catggtgggt ggaataaatg taggagcggt gagcagctcg ccgctcgaat tgtagcggaa
     3001 taggcgtatg tctgccgaca gcaggacatc accactgcca tcttgagtga cagcgatcct
     3061 actaccgcga gcattactgg tgttcgtttt gaaattcgcg acccctgcac cggtgtcgtg
     3121 caagcgatac aatggcccga cccagagggt agcatagagg tcccctgtgc cgtcacccgc
     3181 cggagctaag tcccccactg ttggtatatt acatatagca ccaaagccag acatgaatgt
     3241 tgaatcgaga gtcccatcct gattcagacg gaggagactc gacacagata caccgttata
     3301 gacattcagg gttccaccga cgtagagctt cccgctcgat gtcggcatca gcgcttctat
     3361 agtctgaggg cagcccatac ttgaggtgct tgggaagcta tttcccgtgg agaaagccgg
     3421 atcgac
//
LOCUS       DATIPF010000343         3657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_66415, whole genome shotgun sequence.
ACCESSION   DATIPF010000343 DATIPF010000000
VERSION     DATIPF010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66415"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..1028)
                     /locus_tag="VLL06_08585"
     CDS             complement(216..1028)
                     /locus_tag="VLL06_08585"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS31060.1"
                     /translation="MSYRIKVPAKTLPVDEAHMLSWLDHTVHSSQAYLRPLLISLGVV
                     ILAAAVVGGVFYVDHQAAQKAQDLEREAMRFLTAPSASDPQKADQTLKEAIAKYRQIV
                     DQYPRTATAPLALYYLGNTLVQANDQSGAIEAYQRFLASYSSNPSMAGLVQQRLAYLY
                     LLKGDRDQAVKALTGILETPGTLNRDQALFELARLEESQSRPEAALARYQELIKTYPN
                     SPFTSEATIRTKIMDVKKSQDSAPVSTSTAPTSAPVQKTPSVPPSSPENKNP"
     gene            1180..2340
                     /gene="bioF"
                     /locus_tag="VLL06_08590"
     CDS             1180..2340
                     /gene="bioF"
                     /locus_tag="VLL06_08590"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HSS31061.1"
                     /translation="MHEKRLHELAAQHLTRHITPLHSGVGPVVEMAGRQILLLASNDY
                     LGMAMHPEVVQSAIEATQRFGTGAGAARLISGSLPPHQELESVLAQFKGTEAALTFSS
                     GYLANIGTIPALIGRGGLILADRLCHASLIDGCRLSAADFRVYRHNDTDHLQSLLSAR
                     RKSRRTLIVTDGLFSMDGDLAPLPELWRLAQNYEADLYIDDAHGTGVMGPHGRGTAEH
                     FGLDADLPFQMGTLGKAFGSSGAYLVGSSTLIHYLINTSRSFIFTTAPSPSTAAAVTA
                     ALRVMQREPERRARLWANRERLFSGLMQLGFRLSHSVSPIMPILVGNAKTAISFAEHL
                     FTEGVYAPAIRPPTVPDNTSRIRVTVTSEHTSNHIDHALTAFQRAGQSAGLI"
     gene            2501..3268
                     /locus_tag="VLL06_08595"
                     /pseudo
     CDS             2501..3268
                     /locus_tag="VLL06_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
     gene            complement(3291..>3657)
                     /locus_tag="VLL06_08600"
     CDS             complement(3291..>3657)
                     /locus_tag="VLL06_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HSS31062.1"
                     /translation="EVVVDDVVHGRVVFQNSELDDLIIARSDGTPTYNFCVVVDDMDM
                     QISHVIRGDDHLNNTPRQLNMLLALGQRPPVYAHLPMILGPDGAKLSKRHGAVSVLQY
                     RDEGFLPEARLLSSSTGSE"
BASE COUNT          816 a         1083 c          944 g          814 t
ORIGIN      
        1 cgactttctc atgatcagcc gctaacctcg cattgacttc atctctgtgc tcgcccgccc
       61 aaccccgacg gctacttcac ccacctaccc agagtctgcc aagagagcct ctttgccctg
      121 ggacgcacct cttcccaagc ctctcgcgtg cgcaatgggc acaactcgtc aagtattgat
      181 tttaattatc tcacctgagt tacagggcta tcccgctagg gattcttatt ttctggtgag
      241 gaaggaggaa ccgagggggt tttctgtacc ggagcactgg ttggagcggt cgacgtcgag
      301 acgggcgctg agtcttgcga tttcttgaca tccatgatct tggtccgaat cgtcgcttca
      361 ctggtgaaag gagaattcgg ataggttttg atcagttctt gatagcgagc caacgccgcc
      421 tcagggcgcg attgtgattc ctccagccgc gccagctcaa ataacgcttg atcccggttc
      481 aaggtaccag gggtctccaa gattccggtg agagccttca cggcttggtc ccggtctccc
      541 ttgagcaagt agaggtacgc caaacgttgt tgcaccagcc ccgccattga gggattcgaa
      601 ctgtaggaag caagaaatcg ctggtaggcc tcgatagccc cgctctggtc attggcctgg
      661 accaacgtat tgcccaaata atataaggcg agtggtgctg tggcagttcg tggatattga
      721 tcgacaattt gccgatactt cgcgatcgct tccttgaggg tctgatcagc cttctgagga
      781 tcgctggcag aaggagccgt caagaagcgc atggcctcac gctccaaatc ttgtgctttc
      841 tgcgcagctt gatggtcaac ataaaagacc cctccgacca cggcggccgc gagtatcacc
      901 actcccagac tgatgagcaa ggggcgtaga tacgcctgtg aactatgcac cgtatggtca
      961 agccagctca acatgtgggc ttcatcgact ggaagcgttt tcgccggaac cttgatacgg
     1021 tagctcatgg atggatacgt cggagagggt gagacctgtt tcctcggatt gtgtgcgcgc
     1081 ttatactaag gatgtcacga ttttgtcaat gaggcaactg ttacgtgtcg gtcaccggaa
     1141 gacacttttt ctcccctctt caacaatggg ccagatcaca tgcatgagaa acgactacat
     1201 gaattggctg cgcaacatct gaccaggcac atcactccgc tccactccgg tgtcggtcct
     1261 gtggttgaga tggcggggcg ccagatcctc ctactcgctt cgaacgatta tttgggaatg
     1321 gctatgcatc cggaagtggt tcagtcggcc atagaggcga cccaacggtt tggcaccggg
     1381 gccggcgccg ctcggttgat ctcaggctcg ctgccaccgc atcaagagtt agagtctgtc
     1441 ctggcacagt tcaaagggac cgaagccgca ctgacgttta gctccggcta cctggccaac
     1501 atcggaacta ttcctgcact gatcggacga ggcgggttga tcctggccga tcgtttatgc
     1561 catgcaagtc tgatcgacgg ctgccgcctg agcgcagccg actttcgagt ctatcgccac
     1621 aatgataccg atcatctaca gtcccttctg tccgcacgac gcaaatcgcg gcggaccctg
     1681 attgtgaccg acggactgtt cagtatggac ggtgaccttg caccgttgcc cgagctctgg
     1741 cggttagccc aaaactatga ggccgacctc tatatcgatg atgcccatgg cacaggtgtg
     1801 atgggaccgc atggacgagg aacggccgaa cacttcggtc ttgatgcaga cctcccgttt
     1861 cagatgggga ccctgggcaa agccttcggt agcagtggcg cctatctcgt tgggtcctcg
     1921 acattgattc actatctcat taacacaagt cggtccttca tctttaccac ggcaccctca
     1981 ccgagtacgg ctgctgcggt cacagctgcc ttgcgagtca tgcaacgaga accggaacga
     2041 cgtgcacgcc tgtgggccaa ccgtgaacgg ttgttctcag gattgatgca actgggattt
     2101 cgcttatccc atagcgtcag tccaattatg ccgatccttg tcggcaatgc aaaaaccgcc
     2161 atctcgtttg ccgaacatct gttcacagaa ggcgtgtatg cgccagcgat tcgtcctcca
     2221 accgttcctg acaacaccag ccgcatccgc gtcacagtga catccgagca cacctccaat
     2281 cacatcgatc acgctctcac cgcatttcag cgcgccgggc aatcggcggg actgatctga
     2341 tcaaaccctt cacctctcta cgtacgccat caaagaattc acgcaatcgc ttccggctat
     2401 tttcttgctt tctatttgcc ctatgacatg atgctcccac gtagtcttga aagccgacgg
     2461 gcatcacctc accattcact cttagcgagg agtcctctac atggatattt cgaagctcct
     2521 aaccttcacg gcaaaggagg gcgcgtcgga ttgccacatc agcgccggcg aaccaccgat
     2581 gatccgtatg aacggggacc tcaagaaact cgatcatccc gcactcacca cagaagagac
     2641 gcactcattg atctatgaca tgatgagcga tacccagcgg aagaattttg aagagaagcg
     2701 cgagtgtgac ttttcctttg aattaggcga catcgcccgg ttccgcgtca atgtcttcat
     2761 ccaaaaccgc gggcttggcg ctgtcttccg aaacattccc accgaaatca ttccgatgga
     2821 aaaactcggc atgcctccga tcgtgcgaca gctctgcgat cgggaaaaag gactgatcct
     2881 tgtgaccggc ccaaccgggt ccggtaaatc gacgacgctc gcgtccatgg tcgattatct
     2941 gaacaacacc ttcgaaggtc acatcattac catcgaagat ccgatcgagt tcatccacaa
     3001 gacgaaaaaa tgtctcgtga atcagcggga gatgggcgtc cacactctgt cgttcgcgaa
     3061 cgcgctgagg gcctcccttc gcgaagaccc ggacatcatc ctcgtcggtg aaatgcgcga
     3121 cctggaaacc atccagctcg cgctgactgc tgcggaaaca ggccacttgg tctttggcac
     3181 gctccacacg tccagtgcgc cgaaaaccgt cgaccgcatc atcgacgcct ttcctccaaa
     3241 ccaacagtcc cagattcgca cacagatcgc agaagctttg gaagcggtca tcactcagac
     3301 cctgttgaag aagaaagcag gcgggcctcg gggagaaacc cctcgtcgcg gtactgcagt
     3361 acgcttacag ccccgtggcg tttcgacagt ttggcgccat cggggcccag gatcatgggc
     3421 aaatgcgcat agaccggcgg ccgctgcccc aatgcgagca acatgttcaa ttgccgcggt
     3481 gtgttgttca agtgatcatc cccccgaatc acatgggaga tctgcatgtc catgtcgtcc
     3541 accacgacgc agaaattata ggtcggcgtg ccgtccgatc gcgcgatgat caaatcatcg
     3601 agttcgctgt tctgaaacac cacgcggccg tgcactacat catcgaccac cacctcg
//
LOCUS       DATIPF010000344         1546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_157120, whole genome shotgun sequence.
ACCESSION   DATIPF010000344 DATIPF010000000
VERSION     DATIPF010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157120"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VLL06_08605"
     CDS             <1..889
                     /locus_tag="VLL06_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase"
                     /protein_id="HSS31063.1"
                     /translation="CRVDPDMILVGNGSTELIHLLPRVLGIKSALIMGPTFEEYARAL
                     LDAGSSVRYVHAEQKKRFRPPLQEVLRQYSAKRSRFEAVFVCNPNNPTGQVMNRSALC
                     ELAEVVGRQQGWLIVDEAFIDYCQTQSVVSMLHAHPRMMVLRSLTKFYAMPGLRIGYL
                     VGPSKVVDQLKDRQPPWSVNSLAQEVALAVLGDEAYARKSRIFMTRERSRVMRGLRAL
                     SSVRVYPSAANFLLIELPVSTCAGEVTDRLAAERLLVRDCSAVPGLTTGMIRVAIRTE
                     KENRRLIAALGACVKKSQS"
     gene            886..>1546
                     /locus_tag="VLL06_08610"
     CDS             886..>1546
                     /locus_tag="VLL06_08610"
                     /inference="COORDINATES: protein motif:HMM:NF014060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HSS31064.1"
                     /translation="MSTLDGPVMQTAFRVTRKTLIVDLGGIRSVLSSAPRSGGMTRAR
                     YVLNHQVSANPIGRDESVPGASARCPDPARTLRALATSLGIRERFVGLMTAVSLEDFI
                     AVREADDQIWVEGFVTVGTSNAVRAGETVMPREVTNNRTHPGTINMILVTNARLSAAA
                     MVGVVQVATEAKTAVLLQAKVKSWTGRPGATGTGTDAVVVVSGNGPSQRYSGTHTVLG
                     AL"
BASE COUNT          293 a          407 c          502 g          344 t
ORIGIN      
        1 gtgcagggtc gacccggaca tgatcctcgt cggaaacggt tcgacggaac tgattcatct
       61 gctgccccgt gtgcttggga ttaagtctgc gctcattatg gggcccacat ttgaggagta
      121 tgcgcgtgcg ttgctggatg caggaagctc ggtccggtac gttcatgccg agcagaagaa
      181 acgttttcgc cctccgctgc aagaggtcct gcggcagtat tctgctaagc ggtcgagatt
      241 cgaggccgtg tttgtatgca atcccaataa tcccaccggc caagtcatga accgctcggc
      301 tctgtgcgag cttgccgagg ttgtcggccg gcaacagggc tggttgatcg tggatgaggc
      361 cttcatcgat tattgtcaga cacagtctgt ggtgtcgatg ctgcatgccc atccccgcat
      421 gatggtcttg cgtagtctga cgaaattcta tgccatgccg gggttgcgga ttggctatct
      481 ggttggcccg agcaaggtcg tggatcagct gaaagaccgt cagccaccct ggtcggtcaa
      541 ttcgctggcc caggaggttg cgctcgcggt actaggtgat gaggcctatg cgagaaagag
      601 ccgcatattt atgacgcgcg aacgttcccg agtcatgcgc gggctccgtg cattgtccag
      661 tgtacgtgtc tatccatccg ccgcgaattt tctgctgatt gagcttcctg tctcgacctg
      721 cgccggtgag gtgaccgatc ggctggctgc tgagcggctc ctcgtccgcg attgttcggc
      781 agtgccgggc ctgacaacag ggatgattcg tgtggccatc aggaccgaga aggagaatcg
      841 acgattgatc gcagcacttg gcgcttgtgt gaagaagagc cagtcatgag cacgctcgac
      901 gggcctgtga tgcagacggc ctttcgagtg acacgcaaga cgctgatcgt cgacttgggc
      961 ggtatccggt ctgttctttc ctccgctcca aggtccggtg ggatgacacg ggccagatat
     1021 gttttgaacc atcaagtttc cgccaacccg attggccgag atgaatctgt cccgggggcg
     1081 agcgcacgat gtcctgatcc tgccaggacg ctgagggcac tggccacctc gctcggtatc
     1141 cgcgagagat ttgtcggtct catgacagcc gtatcgttgg aggacttcat tgcggtgcga
     1201 gaggccgatg accaaatctg ggtcgaaggt tttgtgacgg ttgggacttc gaatgccgtg
     1261 cgggcggggg aaacggtgat gccgagagaa gtcacgaaca atcgcactca tcctggcacg
     1321 atcaacatga tcctcgtgac taacgcccgt ctgtctgcag ccgcaatggt cggggtggtg
     1381 caggtggcga cggaagcgaa gaccgcagtt ctgttgcaag cgaaggtgaa gagttggacc
     1441 ggtcgccctg gggcgacggg aaccgggaca gatgcggttg tggtcgtcag tgggaatgga
     1501 ccgtctcaga ggtacagtgg aacccacacg gttctcggcg ccctcg
//
LOCUS       DATIPF010000345         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_241096, whole genome shotgun sequence.
ACCESSION   DATIPF010000345 DATIPF010000000
VERSION     DATIPF010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241096"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VLL06_08615"
     CDS             complement(<1..657)
                     /locus_tag="VLL06_08615"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSS31065.1"
                     /translation="MTPVQAIIAKELRSYFVSPVVYAVGAVFLLIVGLLAYLYVVFAG
                     AQAIQLTQMQGSAQLNLNDLVFRNLFASTRFILLIILPILTMRLFAEERKLRTFEFLL
                     TSPIRINEIVVGKFMSVFIVFLGLLGLTGFMPLILALFSDFEWYPVLTGYLGLILLGA
                     LFLSVGVLASALTENQIVAAFVSIGLLLVLWLLAGLGSLLGDTAIGQAVSYLSFMEHY
                     D"
     gene            complement(654..1634)
                     /locus_tag="VLL06_08620"
     CDS             complement(654..1634)
                     /locus_tag="VLL06_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSS31066.1"
                     /translation="MIEVQQITKRYGQHTALDRVSFSVAKGEVLAFLGPNGAGKTTTM
                     RILTCFMPASEGVARVAGFDCADQSMEVKRRIGYLPETPPVYLELTVTEYLQFVGRLR
                     GLSGKTLISSMQREIERLGLGTVQHRLIGNLSRGYRQRVGLAQALLHDPQVLILDEPT
                     VGLDPKQIIEIRELIKNLAGSHSVILSTHILPEATAVCQRVVIISGGRIVAEDTPEQL
                     SARLRQSEKISLTLKSPTADTESRLKAVQGVQNVFLSGASGTFLLECELGRDVREEVA
                     RLAVASGWGLLELKAISMTLEDVFLQLTRHEDGLAHDAVVASTESAGQPV"
     gene            1838..2182
                     /locus_tag="VLL06_08625"
     CDS             1838..2182
                     /locus_tag="VLL06_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31067.1"
                     /translation="MTRVRQAWRAAIAISALMMLPSIPADSEAADGRTYRVHGQVVAV
                     NLVQTPHMIVIKTPLSKHDDMTVGAKVTAQTKIVRKGKRIMLQTISVGAPVWLTYIKK
                     KDGLFARVIQVQ"
BASE COUNT          624 a          693 c          741 g          556 t
ORIGIN      
        1 gtcgtagtgc tccataaatg acagatagga gacagcctgt ccgatagccg tatcgccgag
       61 cagtgagcct aagccggcga ggagccacag aaccagtaac agaccaatgc tgacaaaggc
      121 cgcgacgatc tggttttcag ttagtgccga ggccagcacg ccgacggaca ggaagagggc
      181 gccaagcaag atgagcccaa ggtagccggt cagcacggga taccactcga aatcactgaa
      241 gagggcaagg atgagcggca tgaaaccggt cagtcccagt aaacccagaa acacgatgaa
      301 tacactcatg aacttgccga cgacgatttc gttgatgcgg attggagagg tcagcaagaa
      361 ttcaaaggtg cggagtttgc gctcttcggc gaataaccgc atcgtcagga tcggcaggat
      421 aatcaggagg ataaatcgag tgctggcaaa tagattccgg aagaccaaat cgttcagatt
      481 cagttgggca ctcccctgca tctgcgtcag ctgaatcgcc tgagctccgg caaagactac
      541 atagagatag gcgagcagtc ccacaatcag aagaaacacg gcccctacgg catacaccac
      601 gggcgagacg aaatacgaac gaagctcttt tgcaatgatc gcttgaacgg gtgtcatact
      661 ggctggcctg ccgattctgt ggaggcgacc actgcgtcat gcgccaggcc atcctcgtgt
      721 ctggttaatt gaaggaatac gtcttccaac gtcatggaaa tggccttcaa ttcgagcagc
      781 ccccatccac tggcgactgc caggcgagcg acttcttccc gcacgtcccg tcccagctcg
      841 cactccaaca gaaaggtacc ggaggccccg ctcaagaaga cattctggac cccttgcacc
      901 gccttcaggc ggctttcggt gtctgccgtg ggtgacttga gggtgagcga aatcttttcg
      961 gactggcgga ggcgtgctga gagttgttcg ggcgtatctt cggccacgat ccgcccgccg
     1021 ctgatgatca ccacacgctg gcacacagca gtcgcttcgg gaagaatgtg ggtgctcagg
     1081 atgacagaat gggacccggc cagattcttg atcaactccc gaatctcgat aatctgtttc
     1141 ggatcaagcc cgacggtcgg ttcatccaag atcaagacct gtggatcgtg caacagtgct
     1201 tgggccagcc ctacgcgttg gcgatatcct cgcgacaggt tcccgatgag ccggtgttgt
     1261 acggtaccaa gaccaagccg ttcgatttcg cgctgcatgg atgaaatgag ggtttttccg
     1321 ctcaaacccc ggaggcggcc tacgaactga agatactcag tgactgtcaa ttccagatag
     1381 accggcggag tctccggcaa atagccgatc cggcgcttca cctccattga ctggtccgca
     1441 caatcgaacc ctgccacccg tgcgacacct tcactcgccg gcataaagca ggtcaggatg
     1501 cgcatggtgg tggttttccc tgccccattg ggaccgagga atgccaacac ctcgcccttg
     1561 gcgacggaga agctgacacg atcgagagcg gtatgttgtc cataccgctt cgtaatctgt
     1621 tgaacttcaa tcatatgtgg tgattggatc gcgtgagttg gggagtactg cctgcgggtt
     1681 ggtcgtcgga ccctaccagg caacacgtcg gcatcttact cagtcggatt gggccagtca
     1741 acaatatcat gttccgacca cctacaactt tacacgtttt cgccatcctg ctaaagtccg
     1801 ctcacgcatg ggcaagcctg ccagagggga tcactcgatg accagggtgc gtcaagcgtg
     1861 gagagcagca atcgcaatct ctgcattgat gatgctgccc tctattccgg cagatagtga
     1921 ggcagccgac gggcggacct atcgagttca tggccaagtc gtcgccgtga acctcgtcca
     1981 aactcctcat atgattgtga ttaaaacccc gttgagcaag catgacgata tgactgtggg
     2041 ggccaaagtg acagcccaaa ccaagattgt acgaaagggg aagcgaataa tgcttcagac
     2101 catcagtgtg ggggcgccgg tgtggctgac ctacattaaa aagaaggacg ggctgtttgc
     2161 tcgggtcatt caggttcaat agcacttctg ctcatagcac caaacttaaa tgtgcggggg
     2221 aactatgggt ccgggggaca atcgttaaga cagaaggctg cccatcaatc ccacctatcg
     2281 taagtcctag gttgactggc acaagtattt tcttctatac atagatagag tcctctaggt
     2341 taccagccaa gattgctggt tcattgttga acgggtcctt attgacctct ccttttccct
     2401 tcatccaaag gtaggggtta gggctacgca ctggggattg ttggactatc ggtcgggccg
     2461 tactgtgagg gacacaggcc aggatcctgt gtgacggttg gttcaaccag gaacaagagg
     2521 tgtttggtga tcaatcaagc gcatggaagc agtcggcagc tgttatggac agtgggtctc
     2581 agtctgggga tgctcctggg tggctgttcg tggg
//
LOCUS       DATIPF010000346         3842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1130611, whole genome shotgun sequence.
ACCESSION   DATIPF010000346 DATIPF010000000
VERSION     DATIPF010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130611"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VLL06_08630"
     CDS             <1..1108
                     /locus_tag="VLL06_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSS31068.1"
                     /translation="LRSQGAYGICLNLLDLLLLSGKLASKGCGLAPLAEENNDQGAVE
                     MGAVAELLPGALDLTDQAARAQMTRTWKEELPLTPGGTLIEMIDRASAGVIKALFVVG
                     ENPVGSLPPQIGVKEALGKLDLLVCQELFLTETAALAHVVLPAASAMEKSGTFTNTEG
                     HVQAVRPAIDPVGDSRPDWEILSAFSVFLGAPLEYGEAKEILKEIRSVIPGYGSLGPV
                     PTCPTVDPVAVDRYVTEGYRHDLATRYTLSERAVRQEGMVQLGLVQNLFHSGKLSTRS
                     KGLLQIEPSGYLQMNPVDAARFALGAGDRVRLSNKRGEFTTTVKVFDRVPEGLAWFPD
                     HFAQEALPLFDCVIDPDTKVPSFRTAAVSVTKIT"
     gene            1157..2248
                     /gene="nuoH"
                     /locus_tag="VLL06_08635"
     CDS             1157..2248
                     /gene="nuoH"
                     /locus_tag="VLL06_08635"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HSS31069.1"
                     /translation="MTELSLRLAVSFAQILAEMGIVMFTVAVMTLAERKVLGWMQDRM
                     GPMEVGPYGILQPLADGIKLFFKEDIVPAGANKAMFALAPMLTMVVSMIGFAVIPFGP
                     DMTLNIYGLTIKPFVVADINIGILYILAIASIGAYGIILGGWSSNSKYSLLGGLRAAA
                     QVISYELNVGLALVGVLLLAGSLSLVEITEAQAGGFWHWFIWGARPDGYFPIHTQLFA
                     FVVFIISGVAETNRIPFDLPEAESELVAGFFTEYSGMKFAFFVSAEYASMVLVSCVAA
                     SLFLGGWNAPYPGTIFEYVGLEQLAWVEHIAWFAAKVYFFLFMFIWIRATLPRLRYDQ
                     LMRFGWKVMLPIALANIIITAITVYIFPR"
     gene            2329..2868
                     /gene="nuoI"
                     /locus_tag="VLL06_08640"
     CDS             2329..2868
                     /gene="nuoI"
                     /locus_tag="VLL06_08640"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HSS31070.1"
                     /translation="MTFKTWVKTITFYELLVGMKATMSHLLHYKPITLQYPHEKRTLP
                     DNYRGMLALLRYDDGTEKCVGCDLCEAACPSRVIRVVSAEVPGEPTKRFAKEYYMDMT
                     RCLFCGMCVDACPVDALGMTREFEWSVYDKRQLDLNKEQLLAIGDRSFPVREKQLELQ
                     HPNVAFFNVAFKHLPQKPD"
     gene            3112..3630
                     /locus_tag="VLL06_08645"
     CDS             3112..3630
                     /locus_tag="VLL06_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HSS31071.1"
                     /translation="MEQAFFFYFAFVIAVTSVLVVALRNPIYSTLALLIMFFHVAGLY
                     VTLHAEFLAAVQIIVYAGAILVLYLFVVMLLNLKQDVRYHRQWPIAATVGVALVIEAI
                     ILTAMKGWTTPVVAADTETAVGGAGNTEAIGDVLYTTYLFPFEVASLILLVAMIGAVI
                     LAKKDLVEPAES"
     gene            3627..>3842
                     /gene="nuoK"
                     /locus_tag="VLL06_08650"
     CDS             3627..>3842
                     /gene="nuoK"
                     /locus_tag="VLL06_08650"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HSS31072.1"
                     /translation="MTVPLSYYLVLSGIMFLTGVIGVLLRRNIIIILLSVELMLNATN
                     INFVAFSQYFHNVTGQVFVFFTLTVAAA"
BASE COUNT          759 a         1062 c         1119 g          902 t
ORIGIN      
        1 gttacgcagc caaggcgcct atggtatctg tctcaatctt cttgatctgc ttctcctgtc
       61 tggaaagttg gcctcaaagg gctgcggcct tgctcccttg gcggaagaaa ataacgatca
      121 aggagccgtt gaaatgggag cggtggcgga actgctgcca ggagcgctcg acctcacgga
      181 tcaggccgca cgcgcccaga tgacccgtac ctggaaagag gaactccctc tgacacctgg
      241 gggcacgctc atcgagatga tcgaccgggc gagcgccggg gtcatcaagg ctctgttcgt
      301 cgttggagag aatcctgtcg ggagtcttcc gccccagatc ggagtcaaag aggccctggg
      361 caaactcgat cttcttgtct gccaggaact gtttctcacg gaaacggctg cgcttgccca
      421 tgtggtgctt cccgcagcct ctgccatgga aaaatcaggg accttcacca ataccgaagg
      481 ccatgtgcag gccgttcggc ccgcgatcga tccggtgggg gacagccgcc ccgactggga
      541 aatcctgtcg gcgttttcgg tttttctcgg cgcgccgttg gaatatggag aagccaaaga
      601 gatcctcaaa gagatccggt cggtgattcc aggctacgga tcgctcggtc cagtaccgac
      661 ctgtcccacg gtggaccctg tcgccgtcga ccgatacgtg accgagggct accgccacga
      721 tcttgcgact cgatatacac tgtccgagcg agccgtacgt caagagggga tggtccagct
      781 tggactggtc cagaacctgt ttcattcagg gaaactgtcg acccgttcga agggactgct
      841 acagatcgaa ccgagtgggt acctacagat gaatcccgtg gatgccgccc gatttgctct
      901 cggggcagga gatcgggttc gcctttcgaa caagcggggc gaattcacga ccacggtgaa
      961 agtattcgat cgggtacccg agggattggc ctggttcccc gaccatttcg cacaggaggc
     1021 cctaccactc ttcgactgtg tcatcgatcc ggacacgaaa gtcccatcct tccggacggc
     1081 ggctgtgtcc gtgacgaaaa taacgtgaga agacaagaac tcatggtttc tttacttgtt
     1141 atcctggagc gctgttgtga ctgaactaag cttgcgactt gcggtgtcgt tcgcccagat
     1201 actggcggaa atgggtatcg tgatgtttac cgtcgcggtg atgactctgg ctgagcggaa
     1261 ggtactcggc tggatgcagg atcgcatggg cccgatggaa gtcggtccct atggaatact
     1321 ccaaccgctc gccgacggca tcaaattgtt cttcaaagag gatattgtac cagctggcgc
     1381 gaacaaggcc atgtttgccc tggcgccgat gctgaccatg gttgtctcca tgatcggatt
     1441 cgccgtcatc cccttcggcc cggacatgac tctgaacatc tatgggctca cgatcaaacc
     1501 gttcgtcgtt gccgatatca acatcgggat cttgtatatc ctggcgattg cctcgatcgg
     1561 cgcctacggt attatcctgg gaggctggtc atccaacagc aaatattcac tgctgggagg
     1621 tctgcgcgcc gcggctcagg tcatcagcta cgagttgaac gtcgggctcg ccctcgtcgg
     1681 cgtgttgctc ttggcagggt ctctgagcct cgtagagatc acagaagccc aggcaggagg
     1741 gttctggcat tggtttatct ggggagcacg ccccgacgga tacttcccaa tccatacaca
     1801 actattcgcc tttgtcgtct tcatcatttc cggcgtggcc gagaccaatc gtatcccgtt
     1861 tgatctgccg gaggccgaaa gcgagctcgt ggctggattt tttacggaat atagcggtat
     1921 gaagttcgcc ttctttgtca gcgcggaata cgcgagcatg gttctcgtat cctgtgtcgc
     1981 cgcttcgctg tttttgggtg gctggaatgc tccctatccc ggcacgatct tcgagtatgt
     2041 cggcctcgaa cagctggcct gggtggaaca tatcgcctgg tttgcggcga aagtctattt
     2101 cttcctcttc atgtttatct ggatacgggc cacgctgcct cgactgcgct acgatcaatt
     2161 gatgcggttc ggctggaagg tcatgttgcc gattgccttg gccaacatca ttatcacggc
     2221 gatcaccgtg tatattttcc cacggtagag cagaagagct ggcagcttcg gatgtgaaac
     2281 tcgatgcagt cgatggctga aacttgatca gccgaggagg tcgcaaccat gacattcaaa
     2341 acgtgggtca agacgatcac cttctacgag ctcctggtcg ggatgaaagc cacgatgtcg
     2401 catctcctgc actacaagcc gatcaccctg caatatccgc acgaaaaacg gacgctgccg
     2461 gacaactatc gcgggatgct ggcgttgttg cgatacgacg acgggacaga aaaatgtgtc
     2521 ggctgcgatc tgtgcgaggc ggcctgcccg tcgcgggtga tccgcgtggt cagcgcggaa
     2581 gtgccgggtg agccgacgaa gcgcttcgcg aaagaatatt acatggacat gacccgctgt
     2641 ctattttgcg ggatgtgcgt cgatgcctgt ccggtggatg ccctcggtat gacacgtgaa
     2701 ttcgagtggt cggtgtacga caagcggcaa ctggacctca acaaggaaca attgcttgcc
     2761 atcggcgacc ggtcgttccc ggttcgcgag aagcagttgg aactccaaca cccgaatgtg
     2821 gcgtttttca acgtcgcgtt caagcacctg ccgcaaaaac ctgactgaat cgcctgcgct
     2881 cggccctcct cacggttcga ggtcgccgag caggatggcc gcgtaaggcg ggcgggatgg
     2941 gcaggagatg cacggcgggt ggaaccagag cgtgcacagt ggtcttgtgc gcttgcctcg
     3001 cgtggcctat tcgtcgcgcc cttggaccat gaagcgatct tggcaggctc ggtatgggtc
     3061 agaatggcgt tgtgttgaat ttccggctcg gggcgtagag ggaaccgtct gatggaacag
     3121 gcattctttt tctattttgc attcgtgatc gccgtgacct cggtgctcgt cgtcgcgctc
     3181 cgcaatccca tctatagtac gctagcgctg ctgatcatgt tttttcacgt ggcggggctc
     3241 tatgtcacgc tccacgctga attccttgca gccgttcaaa tcatcgtcta cgccggtgcc
     3301 atcttggtcc tctacctctt cgtcgtcatg ttgctgaact tgaaacagga tgtccgctac
     3361 catcggcaat ggcccatcgc tgcgacggtg ggagtggcgc tggtcatcga ggcgatcatc
     3421 cttacggcca tgaagggctg gacaactcct gtcgtggcag ccgacacaga aacggccgtc
     3481 ggcggagccg gcaataccga ggcgattggt gacgtcctct ataccaccta tttgtttccc
     3541 ttcgaggtgg cgtccttgat tttgcttgtc gcgatgatcg gcgcggtcat tctggccaag
     3601 aaagacctgg tggagcctgc ggaatcatga cggtcccgtt gtcctactac ctcgtcctga
     3661 gcggcatcat gttcctgacg ggagtcatcg gggtactcct gcggcggaat atcattatca
     3721 tcttgctgtc ggtggaattg atgttgaacg cgacaaatat taacttcgtc gctttttcgc
     3781 aatactttca caatgtaaca gggcaggtct tcgtcttctt taccctcacc gtcgcagcag
     3841 ca
//
LOCUS       DATIPF010000347         3060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_423843, whole genome shotgun sequence.
ACCESSION   DATIPF010000347 DATIPF010000000
VERSION     DATIPF010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423843"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="VLL06_08655"
     CDS             complement(<1..991)
                     /locus_tag="VLL06_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31073.1"
                     /translation="MSIQPWITCLQSGILIRHLEACYPEQASKVDLKRVMGVAESFQE
                     IQDARAFLTDSSNWIPHSVFRELIKSCELAAGQKDFTYQAALAYYETVKTHSPTIIET
                     IAILLNDVESVFRSVGDWASAYTNYLQLQSFVCPDEVQTLHILSRYLPPVDPGLGNMR
                     LVQGNMEGIAKLDPSVETAACEEAYSQMRLETLVNEFGDAYAITSKSDRITVARRSTG
                     DIVLAGRAITLVPELVPVKEDRAQVPQPRDEQLIARTQPQNHLAVWTVSDTSRPPKDL
                     SSNPESLCTTIRIERGGTLSVGSLSTTIKTGAIYGAPYTHYRLRWTNRPPLANN"
     gene            complement(1220..>3060)
                     /locus_tag="VLL06_08660"
     CDS             complement(1220..>3060)
                     /locus_tag="VLL06_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31074.1"
                     /translation="PVTKARSLIRLVEKIDRMIAQATASSSAFTRWRLILFIVGAICT
                     IAFYKMGWFQTGNGALTGFAVLFLIVAAYHNRLESRMHRLRLWKQIKLVHLARLRLDW
                     AHIPARPSSTPDQHPYAKDLDLVGPHSLLHLLDTTVSSYGQARLASWLLEQPPSSDQW
                     LMRRRLVQELMPRSLFRDRLGLDAQLIGEQEINGRRLEAVLQHAVGFRHLTTVLAIQT
                     LLAFTTIGLGLGALLDWIPGYWMWSFAAYVLLYFKTDQGEELLEHAVGVHFELEKLGA
                     VLGFIERHARHSHAALADLWAPLLVPGVNPPQLVRQAARTLHAISVKAHPLIHLSINA
                     LCPWDLWFTYQLQRIQAQVATHLPTWMDRLAEVEATSALATFAALHPSYRWPDSMTVD
                     PQRNGAQARLFAKNLAHPLIPDTQRIQNDLTLETLGTVILVTGSNMSGKSTFLRTLGI
                     NLCLAQAGAPVCADLFEWTWVRLATCIRVDDSLEAGLSYFYAEVKRLKCLLDETADRS
                     KPPVLFLIDEIFKGTNNRERLIGSRAYISALSQGNGFGLVSTHDLELTDLDKTVPGLR
                     NVHFQETVAAGALQFDYKLRPGPCPTTNALRIMELEGLPVPKGETG"
BASE COUNT          669 a          781 c          952 g          658 t
ORIGIN      
        1 cattgttcgc aagtggcggg cgattcgtcc aacgtaggcg atagtgggta tagggcgcac
       61 catagattgc cccggtcttg attgtagtgc tgagtgagcc aaccgagagg gtgccgcctc
      121 gctcaatgcg aatagtggtg cagagggact cggggttgga ggataggtct ttcggaggcc
      181 ggctggtatc actaaccgtc catacggcga gatgattctg cggttgggtt cttgcgatga
      241 gctgttcatc gcggggctga gggacttggg cgcggtcttc ctttaccgga accaattcgg
      301 gaacgagagt aatcgcgcgt ccggcaagca ctatgtcacc tgttgaccgg cgggcgacag
      361 tgatccgatc acttttcgag gtgatggcat aggcgtcgcc aaactcatta acgagggttt
      421 ccaagcgcat ttgggagtag gcctcttcgc acgcggcggt ttcgacggat ggatccagct
      481 tggcaatacc ttccatattg ccttggacca gacgcatatt tccaagtcca ggatcgacgg
      541 gcggcaagta tcgagacagg atgtgcaggg tctgtacttc atccggacag acaaatgatt
      601 ggagttgtag ataattggta taggcagagg cccagtcgcc gacggagcga aacaccgatt
      661 ccacgtcgtt caaaagaatc gcgatcgtct cgatgatggt tggagaatgg gttttgacgg
      721 tttcgtagta ggccagagca gcctgatagg taaagtcctt ctggcccgcc gccaactcac
      781 agctcttgat caactcgcga aacaccgaat gtggaatcca gttgcttgaa tcggtcagga
      841 acgcacgggc atcctgaatc tcctggaatg attcggctac gcccataacc cgtttgagat
      901 cgaccttcga tgcctgttca gggtagcaag cttcgaggtg gcggatgagt attccgcttt
      961 gcaggcaggt aatccaaggt tggatggaca tctatccctc cgcttggtaa acgaggcctc
     1021 aataacttgc agagtcatag cacaacgctg gcagcttagg aagccggcaa gcagtggcac
     1081 aagcttcaac atgccgtcga gctgactcga atcgatgacg ggatgcagca gcctgagtaa
     1141 cggattgcag cgggatttct tcagacgtgg gaggtgtctt ttgtattggg ggcttgccgg
     1201 ttcaaagatc gtgaaccttc tagccggtct ctcctttggg taccggcaat ccctccagtt
     1261 ccatgatccg caatgcgttg gttgtggggc agggacctgg tctgagcttg tagtcgaatt
     1321 gcagcgcgcc ggctgccacc gtttcctgaa agtggacgtt ccgtaatcca ggcaccgtct
     1381 tatcgagatc ggtcaactcc agatcgtggg tactcaccag gccgaagccg ttaccttggg
     1441 agagtgcgga aatgtaggca cgactcccga tcaaccgttc gcgattgttg gtgcctttaa
     1501 agatttcgtc tatcaggaag agtaccggtg gtttggaccg atccgccgtc tcgtcgagca
     1561 ggcattttaa ccgtttcact tcggcataga aatacgacag acctgcttcg agtgagtcat
     1621 ccacgcgaat acacgtggca agtcgcaccc aggtccattc gaaaagatcg gcgcacacag
     1681 gggcgcctgc ctgggcgaga cacagattaa tgcctagcgt tcgcaggaag gtgcttttcc
     1741 ccgacatatt ggaccccgtc accaagatga cggttcccaa tgtttcgagg gtaagatcgt
     1801 tctggattcg ctgtgtatca ggaatcagcg gatgggcgag gtttttggca aagagtctgg
     1861 cctgtgctcc gttgcgctgc ggatcgacgg tcatcgagtc aggccagcga tacgaaggat
     1921 ggagcgcggc aaacgtggcg agtgccgagg tcgcctcgac ctcggccagt cgatccatcc
     1981 aggtcggcag gtgggtggcc acctgagctt gaatccgctg gagttggtag gtgaaccaca
     2041 agtcccaggg acaaagcgcg ttgatgctca gatgaataag tggatgggct ttgacgctga
     2101 tcgcgtgtag ggtgcgggcc gcttgccgga cgagctgggg tgggttcacg cccggcacga
     2161 gcagcggagc ccacaggtcg gcgagcgccg cgtggctatg ccgggcatgt cgttcgataa
     2221 acccaagtac ggctcccagt ttctccagtt cgaagtgcac gcccaccgca tgttccaata
     2281 actcctcacc ttgatcggtc ttgaaataga gcaagacata ggctgcgaac gaccacatcc
     2341 aataacccgg aatccaatcg agcagcgcgc ccaacccgag cccgatggtg gtgaaggcaa
     2401 ggagcgtttg aatggctaag acagtcgtga gatgacggaa tccgactgca tgttgcagga
     2461 ccgcttccag tcgtcgaccg ttgatttcct gttcgccaat gagctgggca tcgagcccca
     2521 gccgatcacg gaacagcgat ctcggcatga gttcctgcac gaggcgacgg cgcatgagcc
     2581 actggtctga gctaggcggc tgctcaagaa gccacgacgc cagtcgcgcc tgtccgtacg
     2641 aggagacggt ggtgtcaagc agatggagca gcgagtgggg gccgaccagg tccaggtctt
     2701 tggcatacgg atgctggtca ggagtgcttg atggacgagc gggaatatga gcccaatcca
     2761 accggagacg tgccaggtgc acgagcttga tctgtttcca gagacgaagc cgatgcatgc
     2821 gactttcgag cctgttatga taggcggcga caattaggaa caggacggcg aagccggtga
     2881 gcgccccatt gccggtttga aaccatccca ttttgtagaa ggcgatggtg cagattgctc
     2941 cgacgatgaa caggatgaga cgccagcggg tgaatgcgga gctcgaggca gtggcctggg
     3001 cgatcatgcg gtcaattttc tcgacaagac gtatcagtga acgggctttt gtgactggcg
//
LOCUS       DATIPF010000348         5921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_953425, whole genome shotgun sequence.
ACCESSION   DATIPF010000348 DATIPF010000000
VERSION     DATIPF010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953425"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VLL06_08665"
     CDS             complement(<1..281)
                     /locus_tag="VLL06_08665"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HSS31075.1"
                     /translation="MPIIVSIASGKGGVGKSVIAANLALLLAKPGKRVTLADLDIGGA
                     DIHILFGHMNPPLTLTDFLNRRVERLEDIVQALPLHPGLTLIPGTGDTLA"
     gene            466..684
                     /locus_tag="VLL06_08670"
     CDS             466..684
                     /locus_tag="VLL06_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSS31076.1"
                     /translation="MSASTMFQPNGAPSTDNEILTVMDVARFLRVPKSTVYKLARVGE
                     LPASKIGKHWRFLRRDIHEWMHSRSQQA"
     gene            1185..1565
                     /gene="cheY"
                     /locus_tag="VLL06_08675"
     CDS             1185..1565
                     /gene="cheY"
                     /locus_tag="VLL06_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HSS31077.1"
                     /translation="MPADPNMKILVVDDMSTMRRIVKNIMKQLGYANVDEAENGKDAL
                     DKLKAESFGFVISDWNMPVMTGIELLRAIRADDQLKALPVLMVTAEAQKENLVEAIQA
                     GVSNYIVKPFTAEVLQEKMNKIFK"
     gene            1643..2230
                     /locus_tag="VLL06_08680"
     CDS             1643..2230
                     /locus_tag="VLL06_08680"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HSS31078.1"
                     /translation="MLNGNVKLYAELGELARYLDSAMKKIAEVGAPMVANSAQLPQAA
                     AHLLDLNKMTEAGTLEVMRLAELIQDNRARAAKELAAVVSTLEAVDCRTLAASLGKTA
                     QELALDDKHLMDIMTALSFQDLVAQRVKKLVTIVEDVHCKLLELVVVFGLKQEGTAPE
                     TQGKADEMLKQLEASKSTAIKQELADELLSEFGFK"
     gene            2377..3147
                     /locus_tag="VLL06_08685"
     CDS             2377..3147
                     /locus_tag="VLL06_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HSS31079.1"
                     /translation="MDIATIIGIVLALGSILGGQVLEGGHPGSIIQLTAFVIVMGGTI
                     GAVCVQNPLSVVLRGVSLLKLGITDPKHDNKGTITTIIDLANVSRKQGLLALEGKLKD
                     IHDPFFRKGVQLIVDGTDPKAVHEILEIDVEFQEEEGLKAAKVWEAAGGYAPTIGIIG
                     AVLGLIHVMENLADPSKLGSGIAVAFVATVYGVGAANLFFLPLASKLKFKLKEEAASR
                     VMIIMGLVGLAQGENPRLLQEKLEGYLPESERTKEDKK"
     gene            3245..4117
                     /locus_tag="VLL06_08690"
     CDS             3245..4117
                     /locus_tag="VLL06_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HSS31080.1"
                     /translation="MAKKKHEEHENHERWLVSYADFITLLFAFFVVMYSISSVNEGKF
                     RTVSESIKAALHPIVSTPSTALPFTVGQNRALTVNPAIEGAKEPAVRRLRQIMRSLKN
                     ETHFEIIELKELTNGDIVLTLPDTVLFRSGESALRPQARPFIQAISEVLIELNRHVRV
                     EGHTDNVPIMTVQFPSNWELSATRAVTVVRVLSEEYGVQADHLTAVGHADSRPLADNL
                     TPENRAKNRRVEIVVQERRPIPQPIETEEPLTALEMFAAPQDSVKREMGASPGNVPPA
                     LQPSSSPAEPAIRP"
     gene            4210..4473
                     /locus_tag="VLL06_08695"
     CDS             4210..4473
                     /locus_tag="VLL06_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSS31081.1"
                     /translation="METNDKLCKPSGELTIFEAEEFKKSLMRILAGEGLVCLDLSQVT
                     RVDTAAIQLMWAARKLGRLLVTGIPQELETKLTQLGFSEPLSE"
     gene            4546..5166
                     /locus_tag="VLL06_08700"
     CDS             4546..5166
                     /locus_tag="VLL06_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31082.1"
                     /translation="MTTPWVEYCLWALVGAVTGAALTAWTLREAYGQGRSETAGSETN
                     ELSRLKEHRQAWEAFAICATHLIPVLKKQMEGVTQQTERAAMDLMVHMKALTLPTPDS
                     TPEETSTNLTKVVMAMQFQDITQQKLEHVAQALDQWHTHLNALLKGPQDESARREIAL
                     LQQLEESYTMEEERRLHAAALAPDYQEPVPIETEPTEIKSESVTLF"
     gene            5235..5606
                     /locus_tag="VLL06_08705"
     CDS             5235..5606
                     /locus_tag="VLL06_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS31083.1"
                     /translation="MGKTALVVDDSPTMRQMVALTLTNAGFKVVEAEHGKDAVNKVAG
                     GSKMDIVVTDLNMPEMDGITLIKELRKMAAFKFTPILMLTTESAEDKKKAGKEAGATG
                     WIVKPFNPELMLKVIAKVLPA"
     gene            5686..>5921
                     /locus_tag="VLL06_08710"
     CDS             5686..>5921
                     /locus_tag="VLL06_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HSS31084.1"
                     /translation="MSNDFAQFQDAFFEEAAEHLAIVEEGLLALEQHPEDRDLLNKIF
                     RSAHSIKGTSGMFGFNAVAQFTHKMETLLDLLRNG"
BASE COUNT         1424 a         1494 c         1776 g         1227 t
ORIGIN      
        1 gcgagcgtat ctcccgttcc gggaatgagc gtgagacccg gatggagggg aagggcctgc
       61 acgatgtcct cgagccgttc cactcgccgg ttcaggaaat ccgtcagcgt gagcggggga
      121 ttcatgtgac cgaacaagat gtggatatcg gctccgccga tatccaggtc ggcgagtgtc
      181 acacgtttgc ccggttttgc cagtaacagg gccagattgg cggcgatcac actcttgccg
      241 acgccgccct taccggacgc gatagagacg atgatgggca ttaagggagc ttcctactgg
      301 ttcgtggcgg tttgtactcc gttgtcggct gttccagaaa aaacatgagc gatcgtgtca
      361 cgtacagagg acgccgggag aatcgcccat tgcggcaatg aacgttatcg gtaactcaag
      421 tttcctatcg gcagcgccga tatcccattc acgatgaaca acgcagtgtc tgcatcaacg
      481 atgtttcaac cgaacggtgc gccatcaacg gacaacgaga tcttgacggt catggacgtg
      541 gcccgttttc ttcgggtgcc caagtccacc gtgtacaagc ttgctcgggt cggtgaattg
      601 ccggcgtcga agattgggaa gcattggcgc tttctccgtc gcgacatcca tgaatggatg
      661 cacagtcgct cccagcaggc ttaacgatcc aatctctgtc tgaggtggtc gcctgtttgg
      721 aaagggtgaa gacgtgtgtc taccgaccgc ttgtcgtgtg cggttagggc aacagacatg
      781 ctcgtggcgg aggggagtca ttgatgagcc ggccagcctg catgattcaa gcagttcgtg
      841 gagaatccgt accggactca ttttgcgcga agcatctctc gcctctcgca tgcaatggcg
      901 taggcccact caattcgcca tacatggcac gcgttccgaa cgaggtgcgg caagcatgtc
      961 atggcgacga accacgaatg agtgagacag gcgtgtgatc atgcgggctg ccggatgaca
     1021 tggcaaccgg ccgtgtcgat gtgatggctc ggtcctgttg tcggctcgtg tgcggtgtat
     1081 tcgcgtgatc atggagaccg gttcatcccg cacgaagaag caagaaggcc cctgcggtat
     1141 cggtggggca atcgacgagg gagaagaata aggaggttga gaccatgccg gccgatccga
     1201 acatgaagat tctcgtggtg gacgatatgt cgaccatgcg aaggatcgtc aagaacatca
     1261 tgaagcaact tgggtatgcg aatgtcgatg aggctgagaa cggcaaagac gcgctcgaca
     1321 agctcaaagc ggaatcgttt ggctttgtca tttccgattg gaacatgccc gtcatgaccg
     1381 gcatcgaact gttgcgagcc attcgtgccg acgaccaatt gaaggccctc cctgtcttaa
     1441 tggtgacggc ggaggcacaa aaggaaaatc ttgtcgaagc gattcaggca ggtgtgagca
     1501 actatatcgt gaagccgttt accgctgaag tgttacaaga aaaaatgaac aagattttta
     1561 aatagctgac ggtgctcatt gatggaacac gcattgccga gggaactacg aagttacgcg
     1621 gtgggacatg aaggaggctg cagtgctgaa tggtaacgtg aaactctacg ccgaacttgg
     1681 cgagttggcc cgctatctcg attccgctat gaagaaaatt gcagaggttg gagctccgat
     1741 ggtggccaac agcgcgcaac tccctcaagc cgcggctcat cttctcgatc tgaacaagat
     1801 gacggaagcc ggcaccctgg aggtgatgcg cttggccgag ctcattcagg ataaccgcgc
     1861 tcgggccgcc aaggaacttg ccgcggtggt gtcaacattg gaagcagtcg attgccgaac
     1921 gcttgccgcg agcctgggga agaccgcgca agagttggca ctggatgaca agcatttgat
     1981 ggatatcatg accgccctgt cgttccaaga ccttgtcgcc caacgggtga agaagcttgt
     2041 caccatcgtc gaggatgtcc attgcaagct gttggaactg gtggtcgtgt tcggtctgaa
     2101 acaagaggga actgcaccgg agactcaggg aaaggccgat gaaatgctca agcaactgga
     2161 ggcgtccaag tccacagcga ttaagcagga gctcgcggac gaactactat ccgaattcgg
     2221 attcaagtag ccatctggtc tgtctggtct attcggtctg tcttgtctat ctggtctatc
     2281 ttgtctcttt cgttcgtcag accaaaggaa ccagatagac caaacagact ggacagacca
     2341 gacagaccgg acatgatgag cgggatgtag gacaaattgg atattgcaac gatcattgga
     2401 attgtgctgg cgcttgggtc gattcttggt ggacaggttc ttgaaggagg ccaccccggt
     2461 tcgatcatcc agctcactgc attcgtcatc gtgatgggcg gaaccatcgg agcggtctgc
     2521 gttcagaatc cgctttcggt ggtgctgaga ggcgtttcct tattgaagct gggtattaca
     2581 gacccgaagc atgacaacaa gggaaccatc accacgatca tcgatttggc caatgtgtcc
     2641 cgcaagcaag gattgctcgc actcgaaggc aagctcaaag atatccatga tccgttcttt
     2701 agaaagggtg tgcaactgat cgtggatggg acagacccga aggcggtgca cgagatcttg
     2761 gagatcgatg ttgagtttca agaagaagaa gggctcaagg cggcgaaggt ctgggaggcc
     2821 gccggagggt atgccccgac tatcgggatt atcggcgcag tcctcggttt gattcacgtc
     2881 atggaaaacc tggcggatcc gtccaagctc ggcagcggca tcgccgtcgc atttgtggcg
     2941 accgtctacg gcgtaggggc ggccaatctg ttctttcttc cgcttgcgag caaactgaaa
     3001 ttcaagctga aggaagaggc cgcctcgcgc gtcatgatca ttatggggct tgtgggattg
     3061 gcacagggcg aaaatccgcg ccttctgcaa gaaaaactcg aaggctattt gccggaatcg
     3121 gaacggacaa aagaggacaa gaagtgaggg ataacagggt aagggggtat cacaacacgg
     3181 tcacttctat ccggctgttc cttctcccgc tcatatgacg gttcctcatc cacgagtggt
     3241 cgctatggca aagaagaagc atgaggaaca tgaaaaccac gaacgctggc tcgtgtccta
     3301 cgcggacttc attacactcc tctttgcgtt ctttgtggtg atgtattcaa tctcgtccgt
     3361 aaacgagggg aagttccgaa cggtgagtga atcgatcaag gccgcgctgc accccatcgt
     3421 tagcacgcca agcactgcac tgcccttcac ggttgggcag aacagagcct tgacggtgaa
     3481 tccggccatt gaaggtgcaa aagaaccggc ggtgcggcgc ctccgccaga ttatgcggtc
     3541 actcaagaat gaaacacatt ttgaaatcat tgagctgaag gaactcacaa acggagatat
     3601 cgtgctgacg ctgcctgaca cggtattgtt tcgcagcgga gaatctgctc ttcggcccca
     3661 ggcccgccca ttcatccagg cgatcagtga agtgctgatc gagctgaatc gtcatgtacg
     3721 tgtggaaggg cataccgaca atgtgccgat catgacggtg caatttccat cgaactggga
     3781 gctgtccgcc acccgcgccg tgacggtcgt acgcgtattg tccgaagagt atggtgtaca
     3841 ggccgatcat ctcaccgcgg tgggtcatgc cgattctcgc cctcttgcgg ataatctgac
     3901 tcctgaaaat cgggccaaga accgacgggt cgagattgtc gtgcaggagc gcaggccgat
     3961 cccgcagccg atcgagacgg aagagccgct cactgccttg gaaatgtttg ccgctcctca
     4021 agactcagtg aaacgggaga tgggtgcgag tcccggtaat gttcctcccg cgttacagcc
     4081 gtcgtcatcc ccggcggaac cggcaatccg tccatgaagg ccgtcaggtg ttccagctct
     4141 caagaatcgc caagaatatc ccgataacga atccatcgac gagcaatcgg aaaggtatca
     4201 ataggcccta tggaaacgaa cgacaagctc tgtaagccat ccggcgagtt gacgatcttt
     4261 gaggcggagg aatttaagaa atcgctgatg aggatactcg caggtgaagg attggtctgc
     4321 ctggatctgt cacaggtcac gcgtgtggat acggctgcga tccagttgat gtgggcggca
     4381 cgcaagctgg gccgtctcct ggtgaccggc attccacaag aactggaaac gaaactgact
     4441 cagctcggat tctccgaacc gctcagcgag tagccgttcg caactggggg tttggcgaga
     4501 gaactatgcc gcccggctgg cgcactaaag ggtaaagatt tcgatatgac gacaccgtgg
     4561 gtggagtatt gcctctgggc actcgttggc gccgtgacgg gagcggccct cacggcttgg
     4621 acattgcgag aggcttacgg tcaggggcgc tccgagacag ccggcagcga gacaaatgaa
     4681 ctcagccggc tcaaagagca tcggcaggcg tgggaagcat ttgccatctg cgcgacacac
     4741 ttgattccgg tcttgaagaa acaaatggaa ggtgtgactc aacagacgga acgagcggcc
     4801 atggatttga tggtgcacat gaaagcgttg acgttgccga ccccggattc aacgccagag
     4861 gaaacatcca cgaatctcac gaaagtcgtg atggcgatgc aattccagga catcacgcaa
     4921 cagaagttgg aacacgtggc tcaggcattg gatcaatggc acacgcacct caatgcgttg
     4981 ctgaagggac cgcaggacga gagcgccagg cgggaaatcg cattactgca acagcttgaa
     5041 gagagctaca ccatggaaga agagcgccgc cttcatgcgg ccgcgttggc gccagattat
     5101 caagaaccag tgccaataga gacggagccg acggaaatca agtcggagtc agtcacgctg
     5161 ttttgagttc ggatcgactg gccatggggg cgacggatcc gctggcttcg agcgataggt
     5221 cgtgggagga agacatgggt aagactgcac tcgtcgtgga cgattcgccg accatgcggc
     5281 agatggtggc gctgaccttg accaatgccg gcttcaaagt tgtggaggcc gaacatggaa
     5341 aggacgctgt caacaaggtc gccggcggat cgaagatgga catcgtcgtg accgatttga
     5401 acatgcccga gatggatgga attactctta tcaaggaact ccggaaaatg gcagccttca
     5461 aattcacgcc gatcctgatg ctcacgacgg aatcagcgga agacaagaag aaagccggca
     5521 aggaggccgg ggcgaccggg tggattgtga agcccttcaa tccagaactg atgctgaagg
     5581 tcattgccaa ggtgttgccg gcgtaatagt catgacaggt gaagagtgct gggtgatcag
     5641 tcagctcctg tagagatgtc gcatcactcg tcataaaggt caactatgag caatgatttc
     5701 gcacaatttc aagatgcctt ttttgaggag gcggcggagc atctggccat cgtcgaggaa
     5761 ggattgctgg cccttgagca acacccggag gaccgcgatc tcctgaacaa aatcttccgt
     5821 tcggcgcatt ccatcaaagg cacgagcggc atgttcggct ttaatgccgt cgcgcagttt
     5881 acccataaga tggagacgct gctcgacttg ctccgcaatg g
//
LOCUS       DATIPF010000349         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1131018, whole genome shotgun sequence.
ACCESSION   DATIPF010000349 DATIPF010000000
VERSION     DATIPF010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131018"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VLL06_08715"
     CDS             complement(<1..480)
                     /locus_tag="VLL06_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HSS31085.1"
                     /translation="MHDTSVTIRTVEFWPVDVPITDPFVVATGARTMAENVFLRVTLA
                     NGARGYGEAAPFPEVGGETRESCLRVLHQLGKAILGRSAADYKEIGLGLSEQALTHPA
                     ARCGLETAVIDAYCRASNIPMWQLWGGAEVRARETDITIPIADLDKTVALARGWYAKG
                     "
     gene            complement(903..1550)
                     /locus_tag="VLL06_08720"
     CDS             complement(903..1550)
                     /locus_tag="VLL06_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSS31086.1"
                     /translation="MVDEPNRLTKTKEQWAKARRGGEEREVFYEGDDRLPPGQHLVET
                     FPVLDLGFKPEVPLTEWTLTIGGFVTNPVTWTWEQFLAQPQFKDVSDFHCVTSWSRFD
                     NEWEGVSFKHILSVVKPLSSTKFVLFKSFDDYTTNLPIEACDAQDVHLTYKWNGKPLT
                     KEHGGPVRVIVPKRYAWKGAKWVKEITFSDKDVKGFWEVRGYSNSAFPWENDRYG"
     gene            complement(1975..2343)
                     /locus_tag="VLL06_08725"
     CDS             complement(1975..2343)
                     /locus_tag="VLL06_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31087.1"
                     /translation="MVTDITIHVESAEGTLERIDHSVTVLVELLKVVVEDLTCLGIWR
                     STSSAITARVIPLRLLEHSMVREVWFRLDDNRWTPFYSCFPMATDLSWSPIGEGLTLC
                     DYSHRLARFSPSRLLGTCFG"
BASE COUNT          666 a          821 c          778 g          639 t
ORIGIN      
        1 ccccttcgca taccatccac gcgcgagggc gacggtcttg tcaaggtcgg caatgggaat
       61 cgtgatgtcc gtctctcgcg ctcgcacttc cgcgccaccc cagagttgcc acatcgggat
      121 gttcgacgcg cggcaatagg catcgatgac ggcggtttcg agaccacaac gtgcggcagg
      181 atgagtgagc gcctgttcag acagccccag cccgatctcc ttgtagtctg cggctgagcg
      241 accaaggatg gctttgccca gttggtgaag gactctaagg cagctctcgc gagtttctcc
      301 tccgacttcc ggaaacggcg ccgcttcgcc ataccctcgt gcaccattcg ccaaggtgac
      361 acgcaggaag acattctccg ccatcgttcg tgcgccggta gcaacgacga agggatcggt
      421 gataggcaca tccaccggcc agaactcgac tgtgcgtatg gtgactgacg tgtcgtgcat
      481 tggaaaagaa aggttctgct ccttagtaga ctgacaaacg ttggccgaat gagtgcatga
      541 ctctgttatg gttctcgtta atggaagggt aatgacaagg ggtctgtctc gtggtgattc
      601 caatggggga agatcggcct tgccagccgt tttgcggacg attaaccgta tggattaaat
      661 cataagggag cgatggaggc aatggccgaa cagcctccgg aaaaactgtt ctggcagacc
      721 agaccttcat tgacctacag gttcgaggta acaggataac atcacgcagg atgctcaaaa
      781 aggctgacca gcaaggccgc aggcgagtcg aaaaccggag gcataccccc gggggtacgc
      841 cgaggatttc gatgagctga gaacgaagct ggcaggcttt ttcagcatcc tgccagacgg
      901 attcacccat atcgatcatt ctcccagggg aaggcgctgt tcgaatagcc ccgcacttcc
      961 cagaacccct ttacatcctt atccgaaaag gtgatctcct tcacccactt ggcgcctttc
     1021 caggcatagc gcttcgggac gatcacacgg accgggcctc cgtgttcttt tgtgaggggt
     1081 ttgccgttcc atttgtaggt caggtggacg tcctgagcgt cgcacgcctc aatcggcaaa
     1141 ttcgtcgtgt agtcgtcgaa tgatttaaag aggacgaact ttgtcgaaga gagcggcttc
     1201 accacggaca gaatgtgttt gaagctgacc ccttcccact cgttgtcgaa tcggctccaa
     1261 ctcgtcacac agtgaaagtc cgagacgtct ttgaactggg gctgcgccag gaattgctcc
     1321 catgtccagg taacggggtt ggtcacaaat ccaccgatgg taagggtcca ttcggttaag
     1381 gggacttcag gcttgaatcc cagatccagt acgggaaagg tctcgacgag gtgttgtccc
     1441 ggtggtaacc gatcgtctcc ctcgtaaaac acttcccgct cttcacctcc acgacgggcc
     1501 ttggcccact gctcctttgt cttggttagt cggttgggtt cgtccaccat ggcctcctca
     1561 tcatagatta cggtaactag ggccttcctg acaactagga gccagtccaa atatgcaaaa
     1621 ataggcagat ttaggacacg cgatcttgcg caatccacga cgctgacaag tacctagata
     1681 gtagatcacc tgtctggagg tcatctgaca ggtgtaggaa ttctctctaa caatcagaga
     1741 cctgtgagtc attataaggc ctggtacact tgaatgattc tagaaaagtc agccgaggga
     1801 atgatggcgt cacgctcata catatgtcgg tttctcctgg caggcgtcct cgtgaccttt
     1861 ggtggtttcg gcgaattaga gccactcgtg ctggctgctt ctgccaagtc gaagaaggat
     1921 gccaaaccgc cagtgactaa gccaccctcg cccgatcgcc aaggttcggc agctctaccc
     1981 aaagcaggta ccaagcaacc ggctaggact gaatcgagca agcctgtggc tgtagtcgca
     2041 cagggtcaaa ccctctccga tagggctcca cgaaagatcc gtcgccatag gaaagcagga
     2101 gtaaaatggc gtccaccggt tatcgtccag ccgaaaccag acctctcgta ccatggaatg
     2161 ctcgagcagc cgcaacggta taaccctagc cgtgatcgcc gagctggtcg agcgccaaat
     2221 ccccaggcag gtgagatcct ccacgaccac tttcaagagc tcgacaagaa ccgtgacgga
     2281 gtgatcgatc cgttcgagcg tgccttcggc cgactcgaca tggatcgtga tgtcagtaac
     2341 catcagtggg aataatcccc ttccagcaaa accctcctac agatcccttt gaatcaaccg
     2401 aaaagaaggg tatcgccatc attctttgcc taggctccta tgtgcattgg tctacttgtc
     2461 atactcttga tcggtttcgg atgcgccggt ccgcaagccc cacaaacccg ggatgttgcc
     2521 aaatcccatg gcgatatctg tccttcccgc ccggtgtttc ctgaaaagga gcgtacgaca
     2581 cagaaaagat gcctttctgc gtcggtaggg tggagtacgg ccggcgaggc cctgagtgga
     2641 gccgccacag ccgtgcggat gccaaaccta tagcggtata cgatccctct cattcaggct
     2701 gtctcaatgg tcatcagcgt gaccagtggc gtttccgtaa atccatctcc ccatctacag
     2761 ctgcacgccc cgtaactgtc atgtgatgca tgggatacgt gcattcatgc tcggctgtgg
     2821 ttcacataac aaggccgatg ctcagcggcc aatctcatgc tcagtcctgt gtctgagaca
     2881 gccatgccga ctcagtcgaa tacc
//
LOCUS       DATIPF010000350         3461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_775723, whole genome shotgun sequence.
ACCESSION   DATIPF010000350 DATIPF010000000
VERSION     DATIPF010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775723"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1247)
                     /locus_tag="VLL06_08730"
     CDS             complement(159..1247)
                     /locus_tag="VLL06_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS31088.1"
                     /translation="MSSTLIPDTVDHKPTVLIVDDEAAPRAALTQILRQDFHILTAEN
                     AHVALAVLDNHGVDLVTLDLKLPDCSGSDLLHDIKHAHAEIEVIMVTAYGSLQSAMDC
                     IRQGAAGFLLKPFNASELLTISLQTAQKKLRLDQLRPLLTNSTTLWGPEPACTKAWDT
                     LVADYTTMNRTPSLSSPQNQETSPLIPLVSDLLEAKDRHLLNHGSRVSFYSTLVANRL
                     SLPIAEQQSLALGALVHDIDLISVQDRQVLNMESNPQLHHADLGAKMGRAMGLSTDAV
                     QIIALHHERWDGTGYPFGLQEELTPLLARIVGIAQVFDELTAEKSGHTSLSFNEALEQ
                     IEQQAGTAFDPLLTEIFCKTMREQPPLQ"
     gene            1512..2606
                     /gene="recA"
                     /locus_tag="VLL06_08735"
     CDS             1512..2606
                     /gene="recA"
                     /locus_tag="VLL06_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HSS31089.1"
                     /translation="MAEKDDKKRALDLALSQIEKQYGKGAIMKLGGADAPVDIPAIST
                     GSLGLDIALGVGGLPRGRVIEIFGPESSGKTTLTLHVIAEAQKAGGTAAFIDAEHALD
                     LTYAKKLGVQVDDLLVSQPDTGEQALEIAETLVRSGAIDVIVVDSVAALVPRAEIEGE
                     MGDAHMGLQARLMSQALRKLTAAISKSQTTLIFINQIRMKIGVMFGNPETTTGGNALK
                     FYSSVRLDIRRIESIKEGQEVTGSRVRVKVVKNKMAPPFKQAEFDIMFAQGISKPGEL
                     VDLGVEKKVVEKSGAWYSYQGDRLGQGRDAVRDFLLSNQPLAREIEGKLRDQAGLPGR
                     TPEKPVVVQVKDEKKTEDKREERRPHKVGV"
     gene            2628..3098
                     /locus_tag="VLL06_08740"
     CDS             2628..3098
                     /locus_tag="VLL06_08740"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HSS31090.1"
                     /translation="MRRVTGKAASSPDRWLQLAVRALARSDRTTAQIERSLSAKGASP
                     SQVRAVVRRLTSLKYLDDVSFAARWADRRLERMPMGRARLQEELLATGCPELIVHATL
                     RAAYRKVSERDLAMHVVTMAGRVSSPQARNRVARLLNQRGFDEDTIETVMGPLM"
     gene            3117..>3461
                     /locus_tag="VLL06_08745"
     CDS             3117..>3461
                     /locus_tag="VLL06_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HSS31091.1"
                     /translation="MKNSTHALRQAFIRYFEQHGHQAVPSSALIPQADPTLLFTNAGM
                     NQFKRVFLGEDTRAYKRAVTVQKCLRAGGKHNDLENVGYTRRHHTFFEMLGNFSFGDY
                     FKEDAIQFGWEFL"
BASE COUNT          747 a          845 c         1119 g          750 t
ORIGIN      
        1 tctcaaggaa ttcatcgaat tggcgcgatc ggtctatcga acgaacgacc gccgggcagc
       61 gatcaaacgg gcgttaaatg tcatgctgaa ctcggaactc atggaagaaa agagctatga
      121 ccggggagct caacaataaa gaacgcgggg ggtctcattc actgcagcgg gggctgctcc
      181 cgcatcgttt tgcaaaatat ttcggtcagc aaggggtcga aggcggtgcc ggcttgttgc
      241 tcgatctgtt ccagggcttc gttgaaggag agggatgtgt gtcctgattt ttctgcggtc
      301 agttcatcaa agacttgggc aataccgaca atgcgagcga gcaggggcgt gagttcttct
      361 tgtaacccaa aggggtatcc cgttccgtcc catcgctcgt gatggagggc gattatctgc
      421 acggcgtcgg tggatagccc catcgctcgg cccatcttcg caccgaggtc ggcatgatgg
      481 agctgcgggt tggactccat gttcagtacc tgacggtcct ggacgctgat caagtcaata
      541 tcatgcacga gcgcgccgag tgcaagcgac tgctgttcgg caatgggaag gctgagtcga
      601 ttggcgacga gtgtcgaata aaaactgacc cggcttccat gattgagcag gtggcgatcc
      661 ttggcttcta ggaggtcgga gaccagggga atcaggggtg atgtctcctg gttctgcggc
      721 gaggagagcg agggcgtgcg gttcatggtg gtgtagtcgg cgacgagagt gtcccatgct
      781 ttcgtacacg caggctccgg gccccagagt gtcgttgagt tggtgaggag cgggcgtagt
      841 tgatcgagcc gcagtttctt ctgggcggtt tgcagggaaa tggtcagcag ttctgaggca
      901 ttgaacggtt tcaggaggaa gcccgccgcg ccctggcgaa tgcagtccat cgctgattga
      961 agactgccgt aagctgtgac catgatgact tcgatttcgg catgcgcatg cttgatgtca
     1021 tgcaagagat ccgatcctga acaatcgggt aatttcaaat ccagtgtgac gagatcgacg
     1081 ccatggttgt cgaggacggc cagggctacg tgtgcatttt cagcggtgag tatgtggaag
     1141 tcctgcctga ggatctgtgt gagggctgcg cgaggcgctg cctcatcgtc gacaatcaga
     1201 acggtcggtt tatggtccac tgtgtcggga atgagagtcg acgacatagt gttatttgta
     1261 tcgtcatacc cgttagtggt caaggaaatc acgtgaatac agggagttgc accggcgatg
     1321 gctgatttat ctcatttatt taattggtcc gtttggtttg tttaattgat cgagacttac
     1381 cagatgaaca acacacacca gatgaaccag atcaactcgt tcccgaacat cgcgcggcta
     1441 tcctgcgggc taggtttctt tctctcaact tgggtataat ccgcgttctc tctaccacac
     1501 gaggagcaac catggctgaa aaagacgata agaagcgcgc gttggatctg gcgttgtccc
     1561 aaattgaaaa acagtatggg aagggggcga ttatgaagct ggggggggcc gatgccccgg
     1621 tggatattcc ggcgatatcg accggatccc tcggactcga cattgctctg ggcgttggcg
     1681 gtcttccccg cggtcgggtg atcgaaattt tcggtcctga gtcttcaggc aagacgacct
     1741 tgacgcttca tgtgattgcg gaggcccaga aggccggcgg cactgcggcc tttatcgatg
     1801 cggagcatgc gctggatttg acctatgcga agaagctggg tgtgcaggtg gacgatctgc
     1861 tcgtgtcgca gcccgatacc ggcgaacagg cgctggaaat tgccgagacg ctcgtccgga
     1921 gcggggcgat cgacgtgatt gtggtggact ccgtcgccgc gctcgtgccg agagccgaaa
     1981 tcgaaggtga aatgggcgat gcccatatgg gattgcaagc gagactgatg tcgcaggccc
     2041 ttcgcaaatt gacggcggcc atttccaagt cgcagacgac cctgattttc atcaatcaga
     2101 tcaggatgaa gatcggcgtg atgttcggca acccggagac gaccaccggc ggcaatgccc
     2161 ttaagttcta ttcgtccgtg cggttggata tccggcgcat cgagtcgatt aaggaagggc
     2221 aggaggtgac cggcagccgc gtgcgcgtca aagtggtgaa gaacaagatg gcacctccgt
     2281 tcaagcaggc ggagttcgac attatgtttg cacaggggat ttcgaaaccc ggcgagttgg
     2341 tcgatcttgg ggtcgagaag aaagtggtgg agaagtccgg ggcctggtat tcctatcagg
     2401 gtgacaggct ggggcaggga cgcgacgccg tgcgagactt tttgttgagc aatcagccgt
     2461 tggcgcgtga aattgaaggg aagctgcggg accaggccgg tctgccggga cgtacgcctg
     2521 aaaagcctgt tgtggtgcag gtgaaagacg aaaaaaagac cgaggacaaa cgcgaggagc
     2581 ggcgaccgca caaggtcggc gtctagtagg aaccgaatca gtctgtggtg aggcgagtaa
     2641 caggcaaggc ggcatcgtct cccgatcggt ggctgcaact tgccgtgcgg gctttggccc
     2701 gcagtgatcg cacgacagcc cagatcgaac gatcgctttc ggcgaagggg gcgtctccct
     2761 cgcaggttcg tgcggtggtt cgacggctga catcgttgaa atacctcgac gatgtctcct
     2821 tcgcggcacg gtgggccgat cggcggctcg aacgcatgcc aatggggcgt gcgcgcctgc
     2881 aagaggaact gctcgccacc ggttgtcccg aactcatcgt acatgcgacg ctgcgggccg
     2941 cctaccgcaa ggtgagtgaa cgagacctgg ccatgcacgt ggtcacgatg gctgggcggg
     3001 tcagttcgcc gcaggcgcgt aaccgagtcg caagactctt gaaccaacgg gggtttgacg
     3061 aagataccat tgaaaccgtg atgggaccat tgatgtgaga gggaagttag ggtggcatga
     3121 agaatagcac tcacgcattg cgacaggcct tcattcgata ttttgaacag catggccacc
     3181 aggccgtgcc cagttccgcc ttgattcctc aggccgatcc gacgttgctc ttcaccaatg
     3241 ccgggatgaa ccagtttaag cgggtattcc tcggcgagga cacgcgtgcc tacaaacgag
     3301 cggtgacggt gcagaagtgc ctgcgtgcgg ggggcaagca caacgatctg gaaaatgtcg
     3361 gctataccag acgccaccat acattctttg agatgttggg gaatttctcc ttcggcgact
     3421 actttaagga agacgcgatt cagtttggtt gggaatttct g
//
LOCUS       DATIPF010000351         4289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1483106, whole genome shotgun sequence.
ACCESSION   DATIPF010000351 DATIPF010000000
VERSION     DATIPF010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483106"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1099
                     /locus_tag="VLL06_08750"
     CDS             173..1099
                     /locus_tag="VLL06_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31092.1"
                     /translation="MNWVRAIAGMLVLWASTAESGMLTWSANGDPDLAGYRVYQCSLL
                     PCTPSSGHATLLATLGKITSFNFGTPAVTQYYFITAYDSSNNESPMSNLGTFTPTGGG
                     TIKLSVLGAPNLPLHGTTAPVTVGISGAMPTLVQLSLDSHPPFASWPSGTFFTLSPDR
                     QSLTGNWCISSACWQGVGGPHVLHVVATYANGATATAAVTINVADAALSLAVAGAPNL
                     PLHGTTAPVTVGISGTMPTLVQLSLDGYPPFATWPSGTFFTLSPDGQSLTGNWCISSA
                     CWQGVGGPHVLHVVATYANGATATAAVTVNVN"
     gene            complement(1749..2909)
                     /locus_tag="VLL06_08755"
     CDS             complement(1749..2909)
                     /locus_tag="VLL06_08755"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HSS31093.1"
                     /translation="MDIRWFRPYLLAILSVFLALQVLWFIQPYISISPPFITFLAAIM
                     VTAWFGGFRPALFATVLSAVIIDYYFIRPPSGFTLSPAEFGTLVFFGGVAAAMAYAID
                     YLQRARHDAAQQVLEAKGQEEELRRAVEDLEATQRGLITELADTEDRERRHLASELHD
                     SLAQLLTLGQMKLKLAERFIGHSPGKSVQYIRETSEAITHSLEYARTLMADLCPPELN
                     DSGLPAAVQWLAGQMSKHGLTVELTLPADPLALPLDRAVLLYRSIRELLMNVVKHAMV
                     NRAMVSMQVDANNTLILRVQDKGGGFDTSSTTGTGKGTHFGLASIRERMTMMGGWCRE
                     ESAIGFGTTITLGLPLSPEIEADSLRAARAPEQDRVKARPKQLPTQENLPLG"
BASE COUNT          972 a         1144 c         1211 g          962 t
ORIGIN      
        1 agccgtcata aacggatcgg tctgatcagc tttccaccag ccccagcagc cgtcgtcgtg
       61 ctggtaacgg aatcttttct gtaatctggc agaagtcatt ctacgtgaga actgagcttt
      121 tccggggatg gtgtacctac agcctggaag caatatcaat aggagtatct gtatgaactg
      181 ggtgagagcc attgcgggta tgcttgtatt gtgggctagt acggccgaga gtggaatgtt
      241 gacctggagt gcgaatggtg acccggacct ggcaggctat cgcgtctatc aatgcagtct
      301 tctaccctgt actccgtcgt ctggtcatgc gacgcttctg gccacattgg gaaagatcac
      361 aagtttcaat tttggcactc cggcagtgac tcagtactat ttcattacgg cgtatgattc
      421 gtcgaataat gagtcgccca tgagcaatct cggaaccttc actcccaccg gtggagggac
      481 cataaaacta tcagtcttgg gtgccccgaa tcttccgctc catgggacca cggccccggt
      541 gacggtgggc atctcgggcg cgatgcccac actcgtgcag ttatcgctgg atagccaccc
      601 gcccttcgcc tcctggccct cgggaacatt tttcacgttg tcgccagacc gccaatcgct
      661 cacaggaaac tggtgcatct cgagtgcgtg ctggcagggg gtcggcggtc cccatgtgct
      721 ccatgtcgtg gccacctatg cgaacggagc gaccgccacc gcggccgtca cgatcaatgt
      781 tgctgatgcc gcgttgtctc tagcagtggc tggtgccccg aatctcccgc ttcatggaac
      841 cacagcgccg gtgacggtgg gcatctcggg cacgatgccc acgctcgtgc agttatcgct
      901 ggatggctac ccgcccttcg ccacctggcc ctcgggaaca tttttcacat tgtcgccaga
      961 cggccaatcg ctcacaggaa actggtgcat ctcgagtgcg tgctggcagg gggtcggcgg
     1021 tccccatgtg ctccatgtcg tggccaccta tgcgaacgga gcgaccgcca ctgcggccgt
     1081 cacagtcaat gtgaactgat tgacgtcgcc ttcaagtatg caggccgctc cgcccgtgaa
     1141 atccttgagc gggcacgaga ggcacaattt ctcgtgcccc attctcgcgc catcgctccg
     1201 caaacctcac cctcgcattg tcgacagaaa ggtctccctt gggaccgact gaggatggga
     1261 attgtattcg ccgttaagcg tgcggtgacg tgtgcagaca gatgcccaag gaagatcggt
     1321 accggcccac ttagctggag gatgttacgc aacgccttgc ctgcgtcgtg ggattccttc
     1381 atactttcgg catgccacga cgcgcaagca agaacgcaat aatagagtat cccgctctga
     1441 tagccagggc ttcgaaagga agaaaggcga gagcaactaa gttgacagca aagaaacaga
     1501 gcctaatagc caaacgcgct gcccaagcaa gatgacaatc ccggtgaacg ccaccgtgct
     1561 tgatcttctg aaagatacat acgcggtacg tcctcggaca actgacctcg tgttctacag
     1621 tcacgcccat acaactctcg atggtgacgt ctttccgggc agtcgatgca gctaaagcgg
     1681 actcgttcgg ggtgggtgtt gtgcagaact tcgatggtgt cgccgtcgaa aaactgatcg
     1741 ttcagtgtct agcccagcgg aaggttttct tgagtaggaa gctgtttagg cctcgccttc
     1801 acacggtcct gttcaggggc acgcgcggcg cggagagaat cagcctctat ctccggtgat
     1861 aatgggaggc ctagcgtgat ggtcgtcccg aagccgatgg cgctctcttc ccgacaccat
     1921 ccacccatca tcgtcatccg ttcgcgaatg ctcgcgaggc caaaatgcgt gcccttcccc
     1981 gttcctgtgg tggaggacgt atcgaaccct cctcccttgt cttgtacccg aaggatgagg
     2041 gtattgttcg catcgacctg catggacacc atcgccctgt taaccatggc atgcttcacc
     2101 acattcatca gcagctcacg gatcgaccga tagaggagta ccgcccgatc taatgggagc
     2161 gccaatgggt cggcggggag ggttaactcc acggtcaatc catgcttgga catttgcccg
     2221 gccagccatt gcacggctgc cggtaaccct gagtcgttga gttcaggtgg acaaaggtcc
     2281 gccatcagcg tccgggcata ttcgagtgag tgggtgatgg cctccgaggt ttcccgaata
     2341 tactggactg attttccagg ggagtggcca ataaatcgtt cggcaagttt caacttcatt
     2401 tgcccgagtg tgagcagctg agcgagcgag tcgtggagtt cggaggccag atgtcgtcgc
     2461 tcacgatcct cggtgtctgc taattcagta atgaggccac gctgggttgc ctccagatct
     2521 tccacggctc ggcgcaattc ttcttcctgg cctttggctt ccaagacctg ctgagctgcg
     2581 tcatgtcgag cacgctgaag ataatcgatc gcataggcca tcgccgcggc gacaccccca
     2641 aagaagacga gggtgccgaa ttccgccgga cttagcgtga agcctgacgg cggacgaata
     2701 aaataataat caatgatgac cgcggacagc acggtcgcga atagcgcagg acggaatccc
     2761 ccgaaccatg cggtgaccat aatggccgca agaaacgtaa taaagggggg tgagatgctg
     2821 atatagggtt ggatgaacca gagcacttgg agtgcaagga aaacacttag aatcgcgagg
     2881 agatagggtc gaaaccagcg tatatccatg gcagcatcgt agaagtcgaa agcccgacgc
     2941 attctagtct gttcacaagt gttagtgcta tagggatcaa gaagggcatc atggcctcgc
     3001 aggggagcgt cctgtaggag gaaaccgaac ggcggcgcaa gggccaccgg cgcgggatca
     3061 ggcgagaatt acgggaaaat tattgtattt aataggcaaa tgtccagacc cgaacgggag
     3121 ggatggtgtg tgcaggtgcc ggattatgtg aggaaggttc agctgtcagt ggagggccta
     3181 ctgcaattca gatgactgtc tcataggtgg agcagcgcca gcctctctaa ccggggtgtt
     3241 cgggtgcgcg aactggggac cgagtgaaag ccgatagatc gagagaccga gatggcgaag
     3301 agaaacccgc ccgtcccgtg acatgcaatc aacggcatcg aatagttgtc cccaactgaa
     3361 atcggaaagg tcgatgacaa gttcgtcaaa ggcgcacggg ccgctccgca gcctgttaat
     3421 aatcgcgtct tctatggtga gcatggcgtc agacctcctg ttgaagtatg tgatacccgc
     3481 attgttgaca gaaatgtgcg ccttggtagc tagggagaac ccttggtctg agcaagggag
     3541 aatccacgcc agtaacctgt gcggattccc tgctggtcag acttccgacg tactgctgtc
     3601 aggaacatga tcaggacggg gatacctgag aaggtcactg tactgcttcg tctaatttct
     3661 ggctaaaggg acacgacaaa tttcgagggt caggtttcaa ccgagtaaga tatcgaagcg
     3721 taacggctgg gagaacgttc gtcaaagaaa cggcggttta aactcgggac gacttgcgaa
     3781 gaaatgtatg ccctaggcat attctctttc cgacgcaact cataggccgg tatttgaggt
     3841 gtccaaaatg tctgtgaatt ctctagctgg taaccttttg atttccttaa gggtcaaaac
     3901 tggaaatcgt aggggccaaa aggaactgtt attgccaacc gcttatgaag ctttaacgtg
     3961 tatcaaagac ctcaggggct tggtctaagt ttgcagacca agtcttctat gtatgagagg
     4021 ggcctttagt ttagatggtt ttctaggacg cacgtggacc gctgtcaccg ccccccactc
     4081 ccgaatgcct cttgcaattg ctagaactgg caggcctgca gctattattc ccaccatttg
     4141 aaacgttcgt tgccgctaaa ctgcaagcag tcaagatcgc ctgctctgag ttgagttcga
     4201 cgcgaaagac ctgaggaggt acataggcct tttttttcat tgcctcactc atggaatctc
     4261 ctctcttgtt cttcccatta gtttcaatc
//
LOCUS       DATIPF010000352         5430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_775952, whole genome shotgun sequence.
ACCESSION   DATIPF010000352 DATIPF010000000
VERSION     DATIPF010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775952"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VLL06_08760"
     CDS             complement(<1..791)
                     /locus_tag="VLL06_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31094.1"
                     /translation="MRHVATSVLFLLCVVSTGSKVYGQNKDACDLVSKADVEAILGQS
                     LYVKSENSQSCAYSNVAPTDPKDDYEALSNTLGGQIRQEGPDTVYMVSENRAKYRIIQ
                     HCKAMNDLTPFRIAFLTPAENSAMQRAAGAAKTKADVNACISEYDAKDISANRKAAIE
                     YCFQANDYTKASNSGVHFDACMNRYDMLQKMCRRQLELQEAYSRRKNPGQLRATQRCP
                     GIRLSPREILAIQAVPIARTMEELPPKFFAPPNVVIPAGPQLPIPA"
     gene            complement(815..2527)
                     /locus_tag="VLL06_08765"
     CDS             complement(815..2527)
                     /locus_tag="VLL06_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31095.1"
                     /translation="MLGRVVVIVVVAAASLWFVEAPSASAAADVTQARYMTPSQFLQS
                     LKQVSLTLHGGSDVLDEYLPVEKAQDMIQNALANRGIVVRPNSPVALEVTLSHERRST
                     KNLDEPVVRHDYFLTLQFFVRGAVWRNGKFHALPVAPASVWWATYVLEPNELQRLLLN
                     EETGKWLRQQFAQGVVDSLNTIDASSMVDTTPFPPHSWSDREKTQGNADFARVMRTDT
                     PMDSSLILELDVEPKLDLTAEFKESECAGGPTFRELWGHEFRQLKWTRQMAQPPLTVE
                     HEFHCRIQKDPRAVAVFHVIHHISLKEANAVFELNGELFRKPVTLQSSEVMTFVNAQD
                     ADEEWFAGLSRRAIFREFDSGLRHGAHERPSLPGAQLSDARRVLGVPTGRMILGEDVR
                     FEHGGWRYANGTQVPDSLLDKATGGPPLRPKSQRPVTQAPVSTYVTDITRTLGAIKSC
                     QLYASASRDNLLWSVLSPALEIDDSGVIVSRYVLSTRSEQGYAQGAAIANLSLIAAQI
                     QDRGGCKILSIPCKNGQCVRFEDNANPSLSVFVETAGQANTILSALKALAPLYPDGAG
                     ALRK"
     gene            complement(2700..3206)
                     /locus_tag="VLL06_08770"
     CDS             complement(2700..3206)
                     /locus_tag="VLL06_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31096.1"
                     /translation="MTDFRLLSWVAIIGVVAVFGSAGTAQGDQATQRAMIAKWAGSYN
                     TEGFLKEPIVRAELQKLLGKELDHLMRNLDVRGGVDLSGETLSVNGNAPHQGTEEEAI
                     VCVTVLPLNTIVEAAFLSKDAVTVYARAEKYENASICIKDWITLVNSGHADRFQQPAN
                     VRVTSPVR"
     gene            complement(3294..4424)
                     /locus_tag="VLL06_08775"
     CDS             complement(3294..4424)
                     /locus_tag="VLL06_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31097.1"
                     /translation="MKRISKVALVTAAILSAVFLTNPAVAGRKPPPDPLTKALQEAGL
                     KQLEDEVVRELGQALEVEAPLRLDRRTAFPPTTIDNFQPKKLAFTPEIMNQPLEPGDY
                     SIDVIGYCTKWSLHYPGRGLPYKLARLQGRQAEAIDALLWRGTFKGLDPRHLKAMAWR
                     IQGGVPISKWRPQEREWAHQLIPDHEASLDGDFVERTRAVYDKARTLGKLPPYETMLS
                     RLGPVGQSVLDLQRARRVLANQTLAAERMPDLLYGRPRDGLPKTLEAGPNEPPSPWSE
                     IRPGVFARVTIIKGDIGHNLLDLRITPQAGIPSVDKESRNRSGFAVIPAAVNIYESTE
                     ELRPSIPYKLFEWFRYLSLLIAYPEGRPAQALILVPYIGIRG"
     gene            complement(4493..>5430)
                     /locus_tag="VLL06_08780"
     CDS             complement(4493..>5430)
                     /locus_tag="VLL06_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31098.1"
                     /translation="AQQSSQPQQQPKYKPGGGTPLLPNVTPQPSPDFGTPEGTAKIAA
                     KAGFLDVVGVKLGMPAKEAVDALKGYNAGFKPTSNTLYEYEALPGVVMTPELVAPNPA
                     GPTASAGDYFNLFVTYAPNEAFLWGIVRNLGFGREENRPTVENTLAGLRKKYGLESTR
                     QPGGERLIWIYDTQGQQVMGAKADDIYSKCSQTWAVNSGGESGGGNRPNANHELSNSI
                     FDQQLKQGYYYGTGGYGSPGGMCHAHSVLDVYYLHATPAGSAADLMTSMTMGAFNRQL
                     EASGVMASHTHLMDAVDKLNEKRGAEESKRGGMKF"
BASE COUNT         1065 a         1622 c         1525 g         1218 t
ORIGIN      
        1 gccggaattg gcagctgtgg cccggcggga atcacgacgt tgggcggggc gaaaaacttc
       61 ggggggagtt cctccatcgt acgagcgatg ggcacagcct gaatagcaag gatctctctg
      121 gggctcaggc ggatgcccgg gcatcgttgt gtcgcgcgca gttgcccggg atttttgcgc
      181 ctcgagtacg cctcctgcaa ttcgagctgc cgcctgcaca tcttctgcag catgtcgtag
      241 cggttcatac aggcgtcgaa gtgaaccccc gagttcgagg cctttgtgta gtcgttcgcc
      301 tggaagcagt attcgatcgc agccttgcga ttcgcgctga tgtcttttgc gtcgtactcg
      361 ctgatgcagg cgttgacgtc cgccttggtc ttggctgctc cggcagcccg ctgcatcgcg
      421 ctgttctcgg cgggcgtgag aaacgcgatg cggaagggag tgaggtcatt catcgccttg
      481 caatgctgga tgatcctata cttcgcacga ttttccgaca ccatgtatac ggtgtccgga
      541 ccttcctgtc tgatctgccc gccgagagtg ttgctcagcg cctcgtagtc gtccttcggg
      601 tcggtcggcg ccacattgct gtaggcgcaa ctctgggaat tttcggattt cacatagagc
      661 gattgtccaa gaatcgcctc gacatccgct ttgctcacca ggtcgcaggc gtccttattc
      721 tgcccataca ctttggagcc ggtgctgacg acacataaga ggaacaggac actcgtggcc
      781 acatgtctca tcgtcacacc tcctgtttcg ctcatcactt ccgcaatgcg cctgcgccgt
      841 ccggatagag cggcgccaga gcctttaacg cgctcagaat tgtgttggct tgcccggctg
      901 tctctacaaa aaccgagaga gacggattgg cattgtcctc aaaccggaca cactggccgt
      961 tcttgcaggg aatgctcaga atcttgcatc cgccgcgatc ctgaatctga gccgcgatga
     1021 gcgacagatt ggcgatggcc gccccttgcg catagccttg ttccgatctg gtggacagca
     1081 catagcggga tacgatcacg ccggagtcgt cgatctccaa agcaggagag aggacggacc
     1141 acaagagatt gtcccttgac gcgctcgcat acaactgaca actcttgatc gcaccgagtg
     1201 tgcgcgtgat gtccgtcacg taggtcgaga ccggcgcttg ggtgaccgga cgttgtgact
     1261 ttggccgcaa cggcgggccg cccgtggctt tgtccagcag tgagtcggga acctgtgttc
     1321 cgttggcata ccgccaccct ccatgctcga accgcacatc ctcgccgagg atcattcttc
     1381 cggtcggcac tcccaacacg cgccgagcat cggaaagctg cgcaccaggg aggctcggcc
     1441 gctcatgtgc gccatgacga aggcccgaat cgaactccct gaagatcgct cggcgagaca
     1501 gaccggcaaa ccattcctcg tcggcatcct gggcgttgac gaacgtcatg acttcagacg
     1561 actggagcgt caccggtttc cggaacagct cgccgttcaa ttcaaagacg gcgttggcct
     1621 ctttcaggga gatgtgatgg atcacgtgga agaccgctac cgcacgagga tccttctgga
     1681 tgcggcaatg aaactcatgc tccacggtga ggggtggttg tgccatctgc cgtgtccatt
     1741 tcaactgccg gaattcgtgg ccccagagtt cacggaacgt tggcccaccc gcgcactcgg
     1801 attctttgaa ttcagccgtg aggtcgagtt ttggctcgac gtcgagctcc aggatcaggc
     1861 tggagtccat cggcgtatcg gtgcgcatga cacgcgcgaa atcggcgttc ccctgtgttt
     1921 tctcccgatc tgaccacgag tgtggtggaa acggcgtagt gtccaccatg gagctggcgt
     1981 cgatcgtatt caggctatcg acaacaccct gggcaaactg ctggcgaagc catttccccg
     2041 tctcctcatt caggagcagc cgctgaagct cgttcggctc caggacatag gtcgcccacc
     2101 agacactcgc cggggccaca ggcaacgcgt gaaatttccc gtttctccat actgcgccgc
     2161 gcacaaagaa ctgcagcgtc aggaaataat cgtgacggac gactggctcg tcgaggttct
     2221 tggtgctgcg acgctcatgc gatagcgtga cctccagcgc cacgggggag ttgggacgca
     2281 cgacaattcc gcgattggcg agcgcattct gaatcatgtc ttgcgccttc tccaccggca
     2341 ggtattcatc caggacatcg cttccgccat gcagggtcag cgaaacctgt ttgagggact
     2401 gcaagaactg actgggcgtc atataccggg cctgtgtgac gtcagcagcc gccgaagccg
     2461 aaggagcctc aacgaaccac aaggacgctg cggcaacgac gacgatcacc acaactcggc
     2521 ccaacatgac attcctcact caggcaagga cgaatcgaaa atggttcctg gtattctccg
     2581 gtctatcaac ttgtccttcg aaccgaatgc cgccggagga gtacggcatc cgttgcttgt
     2641 gacaggagta atcgtagtag ttgtcctcga gatctctgca gtgggctggt catgacacct
     2701 caacgcacag gactggtgac tcgcacattc gccggctgtt gaaatcggtc agcatggccg
     2761 gaattgacga gcgtgatcca atctttgatg caaatacttg cgttctcgta tttttcagcg
     2821 cgcgcgtaga cagtaacggc gtcctttgag aggaatgcag cttccacgat cgtgttgagt
     2881 ggcaagaccg tgacgcagac gatcgcttct tcctccgtcc cctgatgcgg ggcattccca
     2941 ttgacggata gagtttcgcc gctcaggtct acccctcctc tcacatctag gttgcgcatg
     3001 agatggtcca actctttgcc gagtaacttc tggagttctg cacgtacaat cggttccttc
     3061 aaaaatccct cggtgttata gctcccggcc cacttagcaa tcatggcgcg ttgagtggct
     3121 tgatcacctt gcgcagtccc ggcgctcccg aacacagcga cgacgccaat gatggcgacc
     3181 caactaagca atcggaaatc tgtcattgtg tgttgagatc cctgtccacc ctgcggctca
     3241 cctgtactga gctgcctatg actcccaccg accttcatgc ccttattcgg tcgtcatccg
     3301 cggatgccta tgtagggcac gagtatcaag gcctgcgcgg gacgtccttc tggatacgca
     3361 atgagcaagc tgagataccg aaaccattcg aagagcttat agggaatcga tggcctcagt
     3421 tcttcggttg attcatagat attcacggcc gctgggatca cggcgaagcc gcttctattc
     3481 cggctctcct tgtcaaccga aggaatgccg gcctgcggtg tgatacgaag gtccagtaag
     3541 ttgtgcccta tatcgccttt gatgatcgtc acccgcgcaa agacgcctgg acggatttcg
     3601 gaccagggcg agggaggctc gttcggacca gcttccagcg tcttcggcaa tccatctctc
     3661 gggcgaccgt acaataggtc tggcatccgc tccgcagcca gcgtctgatt ggcgaggacg
     3721 cgtcgagcgc gctgcaggtc gagcaccgac tgtccaacgg ggcccaatcg gctcagcatc
     3781 gtttcgtaag gaggtagttt acccaacgta cgcgccttgt cgtagactgc gcgggtccgc
     3841 tcaacaaaat ccccgtccaa cgaggcctcg tgatcgggaa tgagctgatg agcccactcg
     3901 cgctcttgag gcctccactt actaatcggt acgccgccct ggatgcgcca ggccatcgct
     3961 ttcaggtgac gagggtcgag gcccttgaac gttccgcgcc acaacagcgc atcgatggcc
     4021 tcggcctgtc gtccctggag gcgggccaat ttatacggca gaccgcggcc tggataatgg
     4081 agcgaccact tcgtgcagta cccaatcaca tcgatcgagt aatcgcctgg ctccagcggc
     4141 tgattcatga tttccggcgt gaatgcaagc ttcttgggct gaaaattgtc gatggtcgtc
     4201 ggaggaaacg ccgtgcgccg atcgagccgt aacggtgcct cgacttcgag ggcctgaccc
     4261 agttcgcgca cgacttcgtc ttcgagctgc ttgagacccg cttcctgaag agcttttgtt
     4321 aatggatcgg gaggtggctt tcttccggca acagccggat tcgtgaggaa aacggccgac
     4381 aagatcgccg cagtgacgag cgccaccttg gagattcgct tcatgtggct cctcttgttt
     4441 ggattccgcc gcgtatcgta aggcatctct gattccgcac agtcactcag tctcagaact
     4501 tcatgccgcc gcgctttgac tcttccgctc ctcgtttctc gttcaatttg tccaccgcat
     4561 ccatcaggtg ggtgtgggac gccatgacgc cgctcgcctc caattgtcga ttgaatgctc
     4621 ccatcgtcat gctcgtcatg agatccgccg ctgaaccagc cggagtggca tgcaagtaat
     4681 agacgtccaa taccgagtgt gcatggcaca tcccgcccgg agacccatac ccgccggttc
     4741 catagtagta cccctgtttc aactgctgat cgaaaatcga gttggacaat tcatggttgg
     4801 cgttcggacg attaccgcca cctgactctc ccccgctatt cacggcccaa gtctgagagc
     4861 actttgaata gatgtcgtcc gcctttgccc ccatcacttg ctgcccttgc gtatcgtaga
     4921 tccagatgag tcgctcgccg ccgggttgtc gagtgctctc gaggccgtac ttttttctca
     4981 gtccggccaa tgtgttttca actgtcggtc tgttttcttc cctcccgaac ccaagattac
     5041 gcacgatgcc ccagagaaac gcctcgttcg gtgcatacgt aacaaatagg ttgaagtaat
     5101 ctccggccga ggccgtggga ccagcgggat tcggagcgac gagttccggc gtcatcacca
     5161 cgccaggcaa cgcctcatac tcatacagcg tattggatgt cggcttgaac cccgcgttgt
     5221 atcccttcaa ggcgtccacc gcctccttag ccggcatgcc gagcttgacc cccacgacgt
     5281 ctaagaaccc agctttggca gcaatcttgg cggtgccttc cggcgtgccg aaatccggag
     5341 agggttgagg tgtcacgttg gggagaagcg gggtaccgcc acctggtttg tacttcggtt
     5401 gctgctgagg ctgtgatgac tgttgagcct
//
LOCUS       DATIPF010000353         6377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1663117, whole genome shotgun sequence.
ACCESSION   DATIPF010000353 DATIPF010000000
VERSION     DATIPF010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663117"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1305
                     /locus_tag="VLL06_08785"
     CDS             172..1305
                     /locus_tag="VLL06_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31099.1"
                     /translation="MAHSYTPGLTVTEQAVVRRRRMLSLPGNVLVTSGETVRANQAVA
                     RAELPGKVYPLNLANQLSVAPDEIHDYLIKKVGDPIQKDEILAENKPLMKWFKTEILS
                     PVTGVVESVSPVTGQVLLREPPRVFELLGYVDGRIVEVIPQQGVVVETNCSLVQGIFG
                     IGGETRGEIVMAVTSPDEELSTRLFTADMKGKVVVGGSFISSEALARAKELGVAGVVI
                     GGIHDKDLRALLGYDLGVAITGTEQVGFTLILTEGFGSIPMAGKTFALLSAHAGQPAS
                     ISGATQIRAGVIRPEIIIPKGTGAPIASGASAVPEREGIRIGDQVRIIRDPLFGKLGE
                     VASLPSNLQKIPTESEVRVMGVRFADGSTTMIPRANIELIEGA"
     gene            1311..2609
                     /locus_tag="VLL06_08790"
     CDS             1311..2609
                     /locus_tag="VLL06_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HSS31100.1"
                     /translation="MKRETLKVKGRRRRTWLASVLLLVVLLSQSWLPVFAQTDETKKI
                     VPPPQDVRVFDTPSDGGSSLTVLWSPAPDESAASKYQILLSEGTTVSDPAAMKVVVEF
                     AANQRYVRESKWPWWTKPTTGEQHQITLRNGKGVELKDGTAYLVTVARVVGDDRGIAT
                     PVQATPQPNWINWNQMNNLILALLFGGIVFYAIGTARKREIFLRRIPGLAAVDEAIGR
                     ATELGKPILYLTGAHDMHDPSTIAAAIILGRVAKRAAAYETDILVPHRDPITMAVCQE
                     ITKQAYLEAGKPDLYKDDSNFFITSDQFSYTAAVDGIMLRKKPAANFFMGSYFAEALL
                     LTETGASTGAIQIAGTDSDHQLPFFVTTCDYTLIGEELYAASAYLSREPVQVGTLRGQ
                     DIGKALILGVIGVGTILATLGVILGAQWPQLFLDLFKDVK"
     gene            2606..3256
                     /locus_tag="VLL06_08795"
     CDS             2606..3256
                     /locus_tag="VLL06_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31101.1"
                     /translation="MIFLRRQVPLLITLITGIVFAAQYYVPHPASEQMLTSATKWLQI
                     IGGFALVLGVSSLFHVHAVKIRRKEAGWGYSFVLYVSMLGTIAVGLWSNGKEAVDGAM
                     TAFGWIYNFMMVPLQGTMFAILALFIASAAYRSFRARSREASVLLIAAVIVMTGRVPL
                     GEYLIPISGDLSQWILNVLNASVRRAILIGVSLGTVALSIKIIFGLERSYLGGGKE"
     gene            3271..4098
                     /locus_tag="VLL06_08800"
     CDS             3271..4098
                     /locus_tag="VLL06_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31102.1"
                     /translation="MLKIDRRIIFLVIGVCTLLPLLYPVGLPIKISNEVRGVYDHIES
                     LPEGSVFLLSMDFDPASKPELQPQAISLLRHAFKKNLRVVALTLWVSGTGLADQIITQ
                     VAREMGKESGKDYVFLGWSPGGTAVIINMGQDLYGAFPTDYNGKPTKGLPVLQHVANL
                     RDVNYAVSLGAGNPGVEAWYVFGKDKYRFELGGGCTGVMAPGLYPLLRSGQLNGLIGG
                     LRGAAEYETLIGQKGKAIAGMDAQSATHLAIIVLVIMCNLFYFSTRKQGKQQGYSGT"
     gene            4105..4857
                     /locus_tag="VLL06_08805"
     CDS             4105..4857
                     /locus_tag="VLL06_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31103.1"
                     /translation="MSFEMIIGAWVAVGLTLFIFTFLYQDNPLFKLAENLYVGVSVGY
                     TIVKTYDTVIVQLIWKPMVENGEWALLVPVAIGLLMLTRYVPKAAWLSRYAFAFIVGV
                     GSGLAIPRTISSFILKQIEDTVRPLLTLVPGEGVTFTWSLLNPTSSLNTIIILVGVCS
                     VLFYFFFSVEHTGPGKVVARTGILFLMIAFGAAFGYTVMARMSLLIGRLTDLIEFSDP
                     SYGRPSLWLLGLTILILVVMGRLSGSKQPKEE"
     gene            5002..6243
                     /gene="amrB"
                     /locus_tag="VLL06_08810"
     CDS             5002..6243
                     /gene="amrB"
                     /locus_tag="VLL06_08810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HSS31104.1"
                     /translation="MTTGTAKDPTQYPTLRNLQFSPIKEGEEQYMVLWDPTGLSKDKL
                     VLPLNYFFIIQHFDGEHSLAEIGTLYLKRFGEFLGPSRMEQLVSDLNEKLFLEGQRAE
                     DARRLAREAYRQSPLRRAAFAGRGYEADGAKLKKQIDGFFTSKEGPDFKPSEHAGKKI
                     KGLVAPTYDLKQAGSIYAWAYKELQDAEQPDLFVIIGTASAGLDHVFAVTDKDFETPF
                     GIVTADQPILSQLKATLPTFFEADLCHQAEQAVEFQLPFLQDIVGSKKSFTIVPILSS
                     FSASSLEDPTVRQSVDQFLTGLREVLTQSGRAYCVIAAGELAHLGMRYGDKAPPTDFS
                     FHRCMPFDLEMLKPVEERQPEEFANYIRKEQDQRRISGFSPIYSLLRLIEAETGQVLR
                     YDRGIPDQYNSTVTYASMAFY"
BASE COUNT         1306 a         1783 c         1882 g         1406 t
ORIGIN      
        1 gggggcagga gtctcggtca cacgagaggt gcatggcggt gtggtggggc tcttacttga
       61 tggccgcggg cgtccgttgc aattgccgac cgaccacacg gccagggccg catcgctgac
      121 gaaatggtat caggcagtgg atctgtatcc tggctgaccg caatgtagat tatggctcat
      181 tcctacaccc ccggcttaac cgtcaccgag caggcagtag tccgccgccg tcggatgttg
      241 tcgctgcctg gcaacgtact ggtcacgagc ggcgagaccg tacgtgcgaa tcaggctgtg
      301 gcccgtgcgg aattgccggg taaggtctat cccttgaatc tcgccaatca gctgagcgtg
      361 gcgccggatg aaatccacga ctatttgatt aagaaggtcg gtgacccgat acagaaggac
      421 gagattctgg ccgaaaacaa gccgctcatg aagtggttca agacagaaat cctctcgccg
      481 gtcaccggtg ttgtggaatc ggtctccccc gtcacagggc aggtgttgtt gagagagccg
      541 ccgcgcgtat ttgaattact gggctatgtc gacgggcgca ttgtcgaagt gattccgcag
      601 caaggcgtcg tggttgaaac gaattgcagt ctcgtgcagg gcatctttgg catcggcggt
      661 gaaaccagag gtgagattgt catggccgtg acgtcgccgg acgaagagct ctcgacccgc
      721 ctattcaccg cagatatgaa agggaaggtc gtcgtaggcg gctccttcat ctcgtcggag
      781 gcactggctc gtgcgaagga gctgggcgtg gccggcgtgg tcattggcgg cattcatgat
      841 aaggatttgc gcgcactgtt gggctacgac cttggcgtgg ctattacggg tacggaacag
      901 gtcggcttta cgttgatcct cactgaaggg ttcggctcca ttcctatggc cggcaaaacc
      961 tttgcgctcc tctctgctca cgcaggacag ccggcttcga tctcaggtgc cacgcaaatt
     1021 cgagccgggg tcattcgacc ggagatcatc attcccaaag gcacaggcgc accgatcgct
     1081 tccggcgcat cagccgttcc ggagcgggaa ggcatccgga tcggcgacca ggttcgcatc
     1141 attcgagatc cgttgtttgg aaaactcggc gaggtcgcga gtctgccgtc caatctccag
     1201 aagattccga ccgagagtga agtgcgtgtg atgggagtcc gctttgccga cggcagtaca
     1261 accatgattc cgcgtgccaa tattgaattg attgaagggg cctagtgcgc gtgaaacgtg
     1321 aaacgttaaa ggtgaaaggt cgaagacgcc ggacctggct ggcatcggtg ctcctgctcg
     1381 tggttcttct gagtcagtca tggttgccgg tgtttgctca aaccgacgag acgaaaaaga
     1441 tcgtccctcc tccgcaagat gttcgagtgt tcgatacccc gagtgatgga ggcagcagtc
     1501 tgactgtgct ctggtcgccc gctcccgacg agagtgccgc aagcaagtat cagattttac
     1561 tcagtgaagg caccacggtg tcggaccctg ctgcgatgaa ggtggtagtg gagtttgccg
     1621 cgaaccaacg gtacgttcgt gaatccaagt ggccctggtg gacgaagccg acgactggag
     1681 agcagcacca gatcacgctg cggaatggga agggggtcga actcaaggac gggaccgcct
     1741 acctcgtgac tgtggcgagg gtcgtgggtg atgatcgtgg aatcgccacg ccggtgcagg
     1801 cgacgccaca accgaattgg atcaactgga atcagatgaa taacctgata ctggcgctcc
     1861 tgtttggcgg catcgtgttc tacgcgatcg ggacagcgag gaagcgggaa atctttctcc
     1921 ggcgcattcc cggcctcgct gcggtggatg aagccatcgg ccgcgcgacc gagttgggca
     1981 agccgatcct atacctcact ggcgcccacg atatgcacga tccctcgacc attgccgcag
     2041 cgatcattct ggggcgcgtg gcaaaacgcg ccgccgccta tgagaccgat atactcgtgc
     2101 cgcaccgcga tccgatcacc atggccgtgt gccaggaaat tacgaaacaa gcctacctgg
     2161 aagccggtaa gccagatctc tacaaggacg attcaaactt cttcattacg tcggatcaat
     2221 tcagctatac cgcggcggtg gacggcatca tgctgcgcaa aaagcccgca gcgaatttct
     2281 ttatgggatc atactttgcc gaggcgctgc tgttgacgga aacaggcgcg agcaccggcg
     2341 ccatccaaat tgccgggaca gactccgacc accagctccc gttcttcgtc accacctgcg
     2401 attacacctt gatcggggag gaactctacg cggccagcgc ctatctatcc agagaaccgg
     2461 tgcaggtcgg cacattgcgt ggacaggaca tcggcaaggc cctcatcctc ggggtgatcg
     2521 gggtcgggac catactggct accctcgggg tcatcctcgg tgcacagtgg ccgcaattgt
     2581 ttctggacct gttcaaggac gtgaaatgat ctttctccgc cggcaggtcc cgctcttgat
     2641 caccctcatc accggtattg tgttcgccgc gcaatactat gtgccgcatc cggcttcgga
     2701 gcagatgttg acctctgcca cgaagtggct ccagatcatt ggcggttttg cgctggtcct
     2761 gggcgtgagc agcctgttcc acgtccatgc tgtcaagatc cggcgcaagg aagcaggatg
     2821 gggctatagt ttcgtgctct atgtcagcat gctgggcacc attgcagtcg gcctgtggtc
     2881 gaacggcaag gaagccgtcg acggtgcgat gacggcattc gggtggatct ataacttcat
     2941 gatggtcccg ctccagggga cgatgtttgc gatcctcgcg ttgttcatcg cctcggccgc
     3001 ctatcggtcg ttccgcgcgc ggagccggga ggcatcagtg ctgctcatcg ccgcggtgat
     3061 cgtcatgacg ggccgtgtcc ccctcggaga atatctcatt cccatcagcg gcgacctgtc
     3121 tcagtggatc ttgaacgtgc tgaacgcgtc ggttcggcgt gcgatcctga tcggcgtgag
     3181 cctcggcacg gttgcgctct ccatcaagat tattttcggt ctggaacggt cgtatctggg
     3241 gggcgggaaa gagtgacctt ctctgaacgc atgctgaaga tcgaccggcg gatcatcttc
     3301 ctggtgatcg gcgtctgcac gctcttgccg ctgctgtatc cggtcgggtt accgatcaag
     3361 atttccaacg aagtgcgcgg ggtgtatgac cacatcgagt cgctccctga ggggtccgtg
     3421 ttcctgctct cgatggactt cgatccggca tcgaagcctg aacttcagcc tcaagcgatt
     3481 tcattactgc gtcacgcctt caagaagaat cttcgcgtgg tcgcgttgac cctctgggtc
     3541 tccggcaccg gtctggccga ccagatcatc acgcaggttg cccgcgagat ggggaaggaa
     3601 agcgggaagg attacgtgtt cctcgggtgg agccccggcg gcactgccgt catcattaat
     3661 atgggacagg atctctatgg ggccttccca accgactaca acggcaaacc gacgaaggga
     3721 cttccggtgc tccaacacgt cgcaaacctg cgcgatgtga attatgccgt cagtctgggc
     3781 gccggcaatc ccggtgtgga agcctggtat gtgttcggca aggacaagta caggttcgaa
     3841 ctcggtgggg gctgcaccgg agtgatggca ccggggctct atccgctctt gcgcagcggc
     3901 cagctcaacg gcctcatcgg cggcttgcga ggcgcggcgg aatacgagac cctgatcggc
     3961 cagaagggaa aagccattgc cggcatggat gcacaatcgg ctacccatct cgccatcatc
     4021 gtgctcgtca tcatgtgcaa tctgttttat ttttccacgc ggaagcaagg caagcagcaa
     4081 ggctattcgg gaacgtagga acctatgtcg tttgaaatga ttatcggggc ctgggtggcg
     4141 gtgggtctca ccctcttcat tttcacgttc ctgtaccagg acaatccgct gttcaagttg
     4201 gcggaaaacc tctatgtcgg tgtatcggtc gggtacacga tcgtcaaaac gtatgacacg
     4261 gtgatcgtac aactgatctg gaagccgatg gtcgaaaacg gggagtgggc gctcttggta
     4321 ccggttgcca ttggactgct catgctgacg cgctatgttc cgaaagccgc ctggctctct
     4381 cgatacgcgt tcgccttcat tgtcggtgtc ggatcggggc tcgccattcc gcgaacgatc
     4441 tcctccttta tcttgaaaca aatcgaggat acggtccgcc ccttgttgac gctggtgcca
     4501 ggagaaggcg tcacctttac ttggagcctg ctgaatccca ccagcagtct gaacaccatc
     4561 atcattttgg tcggggtctg ttcggtgttg ttttacttct ttttctccgt cgaacacacg
     4621 ggaccgggca aggtcgtggc ccggacaggc atcctctttt tgatgatcgc gttcggcgcg
     4681 gctttcggct acaccgtgat ggcacgcatg tcgctgttga tcgggcgatt gaccgatctc
     4741 atcgagttct ccgatccctc ctatggacgc ccaagtctct ggctcttggg tctgaccatc
     4801 cttatcttag tcgtgatggg ccgactgtct ggcagtaagc aacccaaaga ggaataggtc
     4861 tctctggtct gtcttgtcca tctggtctat ctcgtctgtt cggtctaagg acactaacca
     4921 gatagacaag acagaccgaa cagaccagat agaccacccc agcggttgca ggcgcttctg
     4981 tgcctcaact acaatggccc catgacgacc ggtacagcca aggaccccac acagtacccc
     5041 actcttcgta atcttcaatt ttcgccgatc aaagagggtg aggagcagta catggtcctg
     5101 tgggatccga ccggattgag caaggacaag ctcgttctgc cgctcaacta ttttttcatc
     5161 attcagcatt tcgacggcga gcactcgctg gctgaaatcg ggacgctcta tctcaaacga
     5221 ttcggggaat ttctcggtcc cagcaggatg gagcagttgg tctccgacct gaatgaaaaa
     5281 ttgttcttgg aaggtcagcg tgctgaggat gctcggaggc tggcgcggga agcctatcgg
     5341 cagagtccgc tacgccgcgc ggcctttgcc gggcgggggt acgaagcaga cggtgcgaaa
     5401 ctgaaaaaac agatcgacgg attctttacg tcgaaagagg gaccggactt caaaccgtcg
     5461 gagcatgcgg ggaaaaagat caagggcctc gttgcaccga cttacgatct gaaacaagca
     5521 gggtcgatct atgcctgggc ctataaggaa ctgcaagatg cagagcagcc ggatctgttc
     5581 gtcatcatcg gtacggcctc tgccgggttg gatcatgtgt ttgccgtgac ggataaggat
     5641 ttcgaaacac cgttcgggat cgtcactgcc gaccaaccga tcctgagtca gctcaaggcc
     5701 acgcttccga cgttcttcga agccgatctc tgccatcaag cggagcaggc tgtggaattt
     5761 cagctcccct ttcttcagga cattgtggga agcaagaagt cctttaccat cgtaccgatc
     5821 ttgagttctt tctccgcgtc gagcctggaa gatcccactg ttcgccaatc ggtcgatcag
     5881 tttttgaccg ggcttcggga ggttctcacg caatcgggca gggcctactg cgtgattgca
     5941 gcgggcgaac tggcgcattt agggatgcgt tacggagaca aggcgcctcc gacggacttc
     6001 tcctttcatc gctgtatgcc gttcgatctg gaaatgctga agccggtgga ggagcgacag
     6061 ccggaagaat ttgccaacta tatcagaaaa gaacaggacc aacgccggat ctccggcttc
     6121 tcacccatct attccttact gcggttgatc gaagcggaga ctgggcaggt cctccgctac
     6181 gaccgcggaa tccccgacca atataattcc acggtcacat acgcgtcgat ggccttctat
     6241 tgacagctgc tgaaaaagtc gcccttctca cccgcccaac cccaacgcgc cgagacgcac
     6301 cttttcccat gcttcgttct cgactcatcg aaatcctcaa cgtacccctg agggagctgc
     6361 tgggggttca cgaagga
//
LOCUS       DATIPF010000354         4667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_511826, whole genome shotgun sequence.
ACCESSION   DATIPF010000354 DATIPF010000000
VERSION     DATIPF010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511826"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /gene="pyk"
                     /locus_tag="VLL06_08815"
     CDS             <1..1067
                     /gene="pyk"
                     /locus_tag="VLL06_08815"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HSS31105.1"
                     /translation="GARILIDDGLIELIADRITGGAVECGVVTGGRLTSHKGMNLPDS
                     HISAPTLTDKDRDDLRFGIAQGADYVALSFVRGPDDVVAARKLVADWGDDIPIIAKIE
                     RPEAVERLDAILDQADGVMVARGDLGVEMGPEAVPLLQKRIIAEANRRRRLVITATQM
                     LESMTHHTRPTRAEASDVANAVLDGTDAVMLSAETAIGQYPVETVRVMDRIVRAAEEG
                     TRSWCRRSTDAPQGEGSFPEAICHSASSAASTIGASVIVAFSERGTTARLLSKQRPDA
                     SIISLTPFEDVRQRMALYWGVIPHTMRQIAQTDERIEEAERRLKTERLATTGQRIVIL
                     SGTRVGQPGETNFMKLHKVG"
     gene            1198..1761
                     /locus_tag="VLL06_08820"
     CDS             1198..1761
                     /locus_tag="VLL06_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31106.1"
                     /translation="MTPNIVGRLGFFLCQFLVLVLLAGDGLAQTGSLKHSPADVVKRY
                     LALDHKGARLDAMSFETVASYTSWHDEPTWGHVVVTRGFVVAEQYRQWEVIDSLEVVI
                     PVTFQVLGSVYLETAGFVQETETEEVRFRVKAVKNRWRIVEPMLPPHVGQKRMVNFVR
                     EALLKETDPAKRDRLGALQDELRKAKQ"
     gene            1758..2408
                     /locus_tag="VLL06_08825"
     CDS             1758..2408
                     /locus_tag="VLL06_08825"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HSS31107.1"
                     /translation="MMGKTSVELLIFDLDGTLIESKWDIATSVNLTLAELGLPQRPTE
                     EIFGFVGDGVKQLLRLSVGEGGRGTFDEALRVFRAHYLAHCLDHTTFYPGVGEMLAHF
                     AGKRKAVATNKSIEYTNVILQGLGANHFQYIVGGDNGFGLKPEPGMLLHVMERLNVPK
                     EQTVLVGDSTNDINGGHNAGIRVCAVGYGMGNRAKMEACRPDWFIERPEQLMELFI"
     gene            2594..4027
                     /locus_tag="VLL06_08830"
     CDS             2594..4027
                     /locus_tag="VLL06_08830"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSS31108.1"
                     /translation="MVERHQTPVVSLNMTFGVGGVNEQVGQTGLAHLYEHMAFKGTRT
                     VGTKNYEKEQPVLEEWYRVGTELEQRQRDLARRSQEKPGSPEDRAVIESLQKRFMELQ
                     EQAGQFVAGNEMALLYQRQGGVGLNAATGKDLTRYMINLPANRLPLWAALEADRMAHP
                     VLREFYKERGVVMEERRLRNDDSPSGLLYETFTSAAFRAHPYGIPTIGWESDILSLTP
                     ADTEAFFKAHYGPGNATIAIVGDIHPPDVMVLIEQTFGKIPSAPPQPPIVTVEPPQRG
                     ERRVEVEFDAEPSVVIGFHKPALGHPDDYVFDVIDAVLGDGLTSRLYTNLVRENRIAA
                     SVNSDSNYPGVRSPNLFVLSATPLAPHTTAEIEAAIYAELERLKTEPVSAKELEKVLS
                     NLDADLVRALRSNGGLASQLALYQTVAGDWRYVVKARDKIATVRPADIQRVAAEYFTK
                     SNRTVATLVKKAGDKRVVTALPIEVAR"
     gene            4024..>4667
                     /locus_tag="VLL06_08835"
     CDS             4024..>4667
                     /locus_tag="VLL06_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HSS31109.1"
                     /translation="MRGVIRDAQNLTRWGVVLCSIVIMFTIPVLAGCAGNVALSDPRQ
                     MTFPPVEFTPPEPARVVLENGMVVYFLEDHELPLINMTATIRTGSWLDPTDKIGLASL
                     TGSVMRTGGGGELSSKQVDEELEQFAGDISISIGRQSGSASLDVLSKDVKRGLQIFER
                     LIQTPAFEPARVELAKLQAIEGIRRRQDNPGSIVGREFLKLLYGANHPTARESS"
BASE COUNT         1031 a         1210 c         1482 g          944 t
ORIGIN      
        1 caggggcccg cattctgatt gacgatgggt taatcgagtt gattgctgat cggattactg
       61 gcggcgccgt cgaatgcgga gtcgtgactg gcggacggct tacctcgcac aagggcatga
      121 acctgccgga tagtcacatt agcgccccga ctctgacaga caaagatcga gacgatctcc
      181 ggttcggcat cgcgcagggg gcggactacg tagccctctc gtttgtcaga ggtcccgatg
      241 acgtggtggc cgcaaggaag ttagtggccg actggggcga cgacataccg atcattgcaa
      301 agatcgaaag gccggaagca gttgagcgct tggacgcaat tcttgatcag gcggacggtg
      361 tgatggttgc gcggggcgat ctgggagtgg agatggggcc ggaggccgtt ccgctcctac
      421 agaagcgtat tatcgccgaa gccaatcgtc gtcgccgcct cgtgatcacg gccactcaga
      481 tgttggagtc catgacgcac catacgcgcc cgaccagggc cgaagcatcg gatgtggcta
      541 atgcagtgct cgatgggact gatgccgtca tgttgtctgc agaaacggcg atcggacagt
      601 acccggtgga aacagtccgt gttatggacc gaatcgttcg agctgcagaa gaggggactc
      661 gatcgtggtg taggcgatcg accgatgcac cacaagggga aggctctttt ccggaggcca
      721 tttgccattc ggcctcttcg gcagcctcaa ccatcggagc gagtgtcatc gtggcattta
      781 gcgaaagagg gacgactgcg cgcttgctgt cgaagcagcg acccgacgcg tcaattattt
      841 cattgactcc atttgaagac gtacggcagc gaatggcgct gtactggggg gtgatccctc
      901 acacgatgcg gcagattgct caaactgatg agcggatcga agaagcggaa cgccgtctga
      961 agaccgagag actcgcgaca acgggacagc ggatcgtgat tctgtcgggg accagggttg
     1021 gtcagcccgg agagaccaat ttcatgaagt tacacaaagt cgggtgagct gaagcggctg
     1081 agcgggtcct ctatccggac agtgcctgcg gactgagagc tccattgctg gacccgcgca
     1141 agggaggcct cctccgttta tccaggagga gtgcagattg gaagaagaga tcaaacgatg
     1201 acacccaaca ttgtggggcg gttagggttc tttctgtgtc agttccttgt cctggtattg
     1261 ttagccggag atgggctagc acagaccgga tccctcaagc actcaccggc tgatgtggtg
     1321 aaacgatatc tggcgctcga tcataaaggg gcgaggctcg atgccatgtc gttcgagacg
     1381 gttgcgtcct acacgagttg gcatgacgaa ccgacatggg ggcatgtcgt cgtgacgcgg
     1441 ggcttcgtcg tggcggaaca gtatcgccaa tgggaggtca tcgatagtct ggaagtcgtg
     1501 atcccagtga cctttcaggt cctaggttcg gtgtatctgg aaaccgccgg tttcgtccag
     1561 gagactgaga cggaagaagt tcggtttcgt gtgaaagccg tcaagaatcg atggaggatt
     1621 gtcgaaccga tgcttcctcc ccatgtggga cagaagcgga tggtaaattt cgtgcgcgaa
     1681 gccctgttaa aagaaaccga tccggcaaag cgagatcgtc tcggggcgtt acaggatgaa
     1741 ttgcgaaagg ccaagcaatg atggggaaga cctcggtgga gttgttgatc ttcgatctgg
     1801 acgggacgct catcgagtcc aaatgggaca ttgccacgtc ggtgaacctg acgttggccg
     1861 agctggggct gccgcaacgg cccaccgagg aaatctttgg atttgtgggg gatggggtca
     1921 aacagttgtt gcgcttgtcg gtcggtgagg gcggtcgtgg caccttcgac gaagcattgc
     1981 gggtattcag ggcccactac ctggcccatt gcctggatca caccacgttc tatcccggtg
     2041 ttggagaaat gctggcgcat ttcgcaggca agcggaaggc agtggccacc aataagtcga
     2101 tcgagtatac gaacgtgatt ctccaaggat tgggggcgaa tcattttcag tacatcgtcg
     2161 gaggagataa cgggtttgga ttgaaaccgg aaccgggtat gctgttacat gtgatggaac
     2221 gattgaatgt gccgaaagaa cagacggtgc tcgtcgggga tagtactaac gatatcaacg
     2281 gcgggcataa cgccgggatt cgtgtctgtg cggtcgggta cggcatgggg aatcgcgcca
     2341 agatggaggc ctgtcgtccg gactggttta tcgaacgacc ggaacaactc atggagctct
     2401 tcatatgaaa aacaggtcaa ggttgagatt gagtcaggga gagtcatgtc atcagcatcg
     2461 cctgtgcgct cttgcagcag gtctgtgcat cgtccttagc cttagcccat ctcttcacct
     2521 gtcgcatgcg gcgtcgcttg gtctggcgga tcgtgtggtg gagcagaaat tggcaaacgg
     2581 aatgaccata ctcatggtcg aacgacatca gacgccagtg gtctcgctca acatgacctt
     2641 cggggtcggc ggggtgaacg aacaggtcgg tcaaaccggc ttggcccatc tctatgaaca
     2701 tatggcgttc aaggggacga ggacggtcgg gacaaaaaac tacgagaaag aacagccggt
     2761 tctcgaagag tggtatcgag tggggaccga gctggaacag cggcagcgag atctcgcacg
     2821 gaggagtcag gagaagccgg gcagcccaga ggaccgagcc gtgatcgaga gcctgcaaaa
     2881 acgatttatg gaactacaag aacaggcggg acaattcgtg gcgggcaatg agatggcact
     2941 gctctaccaa cgccaggggg gtgtcgggct caacgcagcg accggcaagg atctgactcg
     3001 ctatatgatc aacctcccgg cgaatcgtct gcccctctgg gctgcgctcg aagcggatcg
     3061 aatggcgcat ccggtcctcc gcgaattcta caaggaacgc ggagtggtta tggaggagcg
     3121 gcggttgcgg aatgacgaca gtcccagcgg attgctgtat gaaaccttca cctcggcggc
     3181 cttccgcgca catccgtatg gcattccgac gatcggttgg gagtcggaca tcctctcgct
     3241 cacgccggcc gacaccgaag ccttctttaa ggcccattac ggtcccggga atgcgaccat
     3301 cgccattgtg ggcgatatcc atccaccgga cgttatggtc ttaatcgagc agacgttcgg
     3361 caagataccg tctgcacctc cgcagcctcc gatcgtcacc gttgagccgc ctcaacgagg
     3421 agagcgccgc gtggaggtgg agttcgatgc tgaaccgtcg gtcgtcatcg ggtttcataa
     3481 gccggcgctg ggtcatcccg acgactatgt cttcgatgtg atcgacgcgg tgttaggcga
     3541 cggactcacg tcccggctct atacgaatct ggtccgcgag aatcggatcg ccgcctccgt
     3601 caactcggat tcgaattacc ccggtgttcg ctccccgaat ctcttcgtgc tcagtgccac
     3661 accgcttgcg ccccatacga cggcagaaat tgaagcggcc atctatgccg agctcgaacg
     3721 actcaaaacg gaaccggtgt ccgccaagga gcttgagaaa gtgctgagca acctcgatgc
     3781 cgatttggtg cgggcgctcc gatccaacgg aggtctggct tctcaattgg cgctctatca
     3841 gacggtcgca ggagactggc gttacgtggt caaggcccgg gacaaaatag ccaccgtgag
     3901 gccggcggat atccagcgag tggcagctga gtatttcacc aagtcgaacc gaacagtggc
     3961 cacgttggtg aagaaggccg gcgacaagcg cgtcgtgacg gcgctgccga ttgaggtggc
     4021 acgatgagag gcgtgatacg tgacgcgcaa aacctgacac gatggggtgt cgtgttgtgc
     4081 tcgatcgtca tcatgttcac gatcccggtg ttggccggct gtgccggaaa tgttgcactg
     4141 agcgacccac ggcagatgac gtttccacca gttgagttca caccgcccga accggctcgc
     4201 gtggtactcg agaacgggat ggtcgtctat ttccttgagg accatgagct gcccttgatc
     4261 aatatgaccg cgaccatacg aacgggcagt tggctcgacc cgacagataa gatcggcctg
     4321 gcttctttga ccggttccgt catgcggacc ggcggcggcg gcgagctgtc gtccaagcag
     4381 gtagacgaag aactcgaaca gtttgccgga gatatcagca tctcgatcgg gcggcaatcc
     4441 gggtctgcat cactcgatgt gctcagcaag gatgtgaaac gcgggctcca gattttcgag
     4501 agactgattc agacacccgc ctttgaaccg gcacgggttg aactggccaa gctgcaggcc
     4561 atcgaaggta ttcgtcgaag gcaggataac cccggttcga tcgtcggccg cgagtttttg
     4621 aagctcctgt atggagcaaa ccacccaacg gcgcgtgaaa gcagcat
//
LOCUS       DATIPF010000355         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_602230, whole genome shotgun sequence.
ACCESSION   DATIPF010000355 DATIPF010000000
VERSION     DATIPF010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602230"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VLL06_08840"
     CDS             complement(<1..324)
                     /locus_tag="VLL06_08840"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HSS31110.1"
                     /translation="MANQHVRITRHLETLVRERHPETSPRALRKAAHYLSTQFSKEGW
                     AASSQLVSAWGKVYRNVVAIKCPDRSGQQEELPPLLIGAHYDTVSGSPGADDNASGLV
                     VLLEVA"
     gene            complement(351..1247)
                     /gene="argB"
                     /locus_tag="VLL06_08845"
     CDS             complement(351..1247)
                     /gene="argB"
                     /locus_tag="VLL06_08845"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HSS31111.1"
                     /translation="MNRLIKKANVLIEALPYIRTFKGKTIVIKYGGHAMTDAPLKERF
                     AQDVVLLKYVGLNPVIVHGGGPQIDKMLDRLGIEAKFRHGVRVTDEATMEIVEMVLAG
                     KINMEIVDLLNRHGGLAVGLSGKDGGLMLSRPLTAKAWAASLEKDLDDGEGDEDFGLV
                     GEVQSIDPTLVRKLQQDHYIPVIAPIGTNREGNTYNINADLVAGAMAAALGAEKLVMM
                     TDVKGIRGTNGHHLSTVSRKDVQRMVKKGTISEGMLPKVHACLDALGGGVGKAHIIDG
                     RIPHAILLEVFTRKGIGTEITS"
     gene            complement(1369..2262)
                     /locus_tag="VLL06_08850"
                     /pseudo
     CDS             complement(1369..2262)
                     /locus_tag="VLL06_08850"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
BASE COUNT          505 a          737 c          656 g          525 t
ORIGIN      
        1 tgccacctcg agaagcacca ccaacccact tgcattatca tccgcaccag gtgatccaga
       61 cacggtgtcg tagtgtgccc cgatcaggag tggaggcaac tcttcttgtt gtccagacct
      121 gtccgggcac ttgatcgcca ccacattgcg gtagaccttg ccccaggcac tcacgagctg
      181 gctcgaggcg gcccatcctt ccttggaaaa ctgcgtcgac agatagtgcg ccgccttccg
      241 aagcgcgcga gggctcgtct caggatgacg ctcgcgaacg agggtctcaa gatggcgggt
      301 gattcggacg tgttggttcg ccacaaaaaa gagactggtt ggaagaacga ctacgacgta
      361 atctcggtcc cgataccctt acgtgtgaat acttcgagga gaatggcatg gggtattcga
      421 ccgtcaatga tatgggcttt gccgacaccg ccgccgaggg catccaagca cgcatgtacc
      481 ttgggcaaca tcccttcgct gatcgtgccc ttcttcacca tccgctgcac atctttgcgg
      541 gaaaccgttg agagatggtg cccgttcgta ccgcgaatgc ctttcacatc agtcatcatc
      601 accaactttt ccgcaccaag tgcggcggcc attgccccgg ccaccagatc ggcattgata
      661 ttgtacgtgt tcccttcccg gttggtaccg atcggtgcaa tgacgggaat ataatgatcc
      721 tgctgaagtt tccgcaccaa cgtgggatcg atggactgga cctctccgac caggccaaaa
      781 tcctcgtccc cctcgccgtc atcgagatcc ttttccagac ttgccgccca ggctttcgca
      841 gtcagcgggc gagagagcat gagccctccg tcctttccac tcaaacccac cgcaaggccg
      901 ccgtggcgat tcaagagatc aacaatctcc atattgattt tcccggccag caccatctcc
      961 acgatttcca tcgtcgcctc atctgtgacc ctcaccccat ggcgaaactt ggcttcgatg
     1021 cctaagcgat caagcatctt atcgatctga ggtcctccgc cgtgcacaat gaccggattg
     1081 agaccgacat atttcaatag cacaacgtcc tgcgcgaagc gctctttcaa tggtgcatcg
     1141 gtcatcgcat gaccgccata tttgatgaca atcgtcttgc ccttgaacgt gcgaatgtag
     1201 ggcaaggctt cgatgaggac gttcgccttc ttaatcagtc tgttcatatt ctggctcccg
     1261 tggcgagaaa agcgtgaacg gcaagacacg cagggcgagt cagttcgcca cccataccac
     1321 ttcccgagtt tcgcgtgaca cagcgtgtca tgagtaatcg catgatgatc acggcttgat
     1381 ggccttcacg acacgcgcac tggccggccg accggcccgc ggatgagtct tatcatagac
     1441 cgccaggatc tggtccgtgg ccagatgcgt atacttttgt gtcgtactca atgaggcatg
     1501 cccgagcatc tcttggatcg atcgcagatc ggccccttca tcgaggaggt gggtggcaaa
     1561 cgagtgacgc aacgtatggg gactcacggg tccgccggca agccggttcg aatagcgggc
     1621 cacaatgcga gcgacgctcc gggtcgtcaa ccgtcctcct cggctgtttc gaaaaaccgg
     1681 cgacggaacg cgcttcgtcg tcttccgatc tcgagacacc agaggcagag gctgctgatt
     1741 cagataggcc tgaatggccc gtagggctac ctctccgatc ggcacgatgc gctccttccg
     1801 gccttttccc cgcagatggg ccagcccctt ggacacacgg agatcttcga gattgatgtc
     1861 cacgagttca ctcacccgag ccccggtcga atagagtgtc tctagcaagg cgcggtctct
     1921 gagcgccccg cctgcctgat cggcgggaaa ctccatcagc gcagccgcat cgtccttggt
     1981 caagacacgc ggaagatgtt tcggccgttt tggggtcctg atggcctcgg cgggattgag
     2041 gccgaccagg ccttcccgtt gcaaatagcg aaagaagctg cggagagagg caagcttccg
     2101 ggccatcgac gtacttttct tgcgtttgcg atccaaccag tgaagatacg cccggctgga
     2161 ctccgtcttc acggtaagag gattgagcgc gagctcgctc ggatgctccg cattcataaa
     2221 ggcctgaaac tgtcgaagat ccgacacata gctacgcacg gtttcatgcg acgcatgacg
     2281 ttccagctca aggaacgtca tgaaagctcg aattgcgtca tccatgccgt caggtcctca
     2341 agcgcccgtt gtcctgtgag ccgtcgtttg cgctccttgt ctctcgtcgg tgtggccagg
     2401 ggaggaaaga gcccaaaatt cgt
//
LOCUS       DATIPF010000356         3224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_689485, whole genome shotgun sequence.
ACCESSION   DATIPF010000356 DATIPF010000000
VERSION     DATIPF010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689485"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VLL06_08855"
     CDS             <1..424
                     /locus_tag="VLL06_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hopanoid C-3 methylase HpnR"
                     /protein_id="HSS31112.1"
                     /translation="SAKIQTRDYRLFDIQHAVVPTRLPLVEFYEELVKTQQVLNMKHL
                     GWAALKTTATLAAGHLMRGQTNFIKMLWKFNSVYNPKLQMADHQRPVSYQMALPPPPR
                     ENLDSKLLYILPSKGRHGRSIDDSTEQFVETTRMGSSL"
     gene            475..681
                     /locus_tag="VLL06_08860"
     CDS             475..681
                     /locus_tag="VLL06_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31113.1"
                     /translation="MMNRPQTLYGIADSPVGLAAWILDHDAHSQAFFAHVFDGQPEGL
                     TRDDILDNGTLYWLTNTAVFLARP"
     gene            1359..2183
                     /locus_tag="VLL06_08865"
     CDS             1359..2183
                     /locus_tag="VLL06_08865"
                     /inference="COORDINATES: protein motif:HMM:NF026037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSS31114.1"
                     /translation="MSKAKIFTYWIVSMLMMELMGTGAGLAIAQPYQVVNVTDGGTIT
                     GVATWKGEVPELPPIEIEADTNVCGEEVPSQALRVNSKNNGVPYVLVYLEGVEKGKAP
                     ADKYWLRMANCQFQEHVFPFVRSQVMAMINHDPFFHNPHFFNAKNASLLNVPMPDPSQ
                     AIEHKFLRTPLKNERGVMRLQCDVHNNMNAYWAAFDHPYFAVTDADGKFEISGIPPGR
                     YTVVAWHEGYKVLIKEATGRPLYDRPHIRQQEIEVKPKETVKVGFEFPVRKVYIDY"
     gene            2253..>3224
                     /locus_tag="VLL06_08870"
     CDS             2253..>3224
                     /locus_tag="VLL06_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HSS31115.1"
                     /translation="MGRRRLVLTRVLVVVLLAGLPVTHSLANPPEADEVKDHPGMEPT
                     VTRGMPDLVRYWNFDPPKSPLFDIGRVTISGDARVRPEYRSNSNFGLASTSGVGRAEG
                     TNSQGDTAQFVQQWARLGLNYAMSPDVDTFFQMQYAANWGANSAPNTANDPNTLTSVG
                     VRQAYILIRNLGVDGLSMKAGRQLIVMGNHRLFGNFDWNNIGFSFDGITLQYSQRMYE
                     VWGGWVRLADAQANAGNSSCGTGTTGCAGGSGAVNPGTVAGGTTGSNNADLFFARIAI
                     KPMEGLSLEPLWVFLDNNISVTAPPVATTYYGTAAHAPGQQRHTLGGR"
BASE COUNT          778 a          900 c          868 g          678 t
ORIGIN      
        1 atcggccaag atccagacgc gggattaccg ccttttcgat attcaacatg ccgtggtgcc
       61 gacaagactc ccgcttgtcg agttctacga agagctcgtc aagacacaac aagtgcttaa
      121 catgaagcat ctcggctggg ccgccctgaa aaccaccgcc acactcgcgg ccgggcatct
      181 catgcgcggg cagaccaact tcatcaagat gctgtggaag ttcaacagcg tctacaaccc
      241 caaactccag atggccgacc accagcgccc ggtgtcatat caaatggcac tcccgcctcc
      301 cccgcgggaa aacttagatt caaagctcct ctacatcctt ccttccaaag gtcgccacgg
      361 acgttctatc gatgactcga cggagcaatt cgtcgaaacc acccgcatgg ggagcagcct
      421 ctaaatcaat ttgaacagag aggacggata ggttgccggg gctacggcca ggcgatgatg
      481 aatcgcccgc agacgctata cgggattgcg gattcaccgg tcggcctagc cgcctggatc
      541 ctcgaccacg acgcacacag tcaggcattc ttcgcacacg tcttcgatgg tcagcccgag
      601 ggcctgacgc gagacgacat cctcgacaac ggcacgctct actggttgac gaatacggcg
      661 gtctttttgg ctcgtccctg atttgggaaa gcaaactagc tttctttgcc ccgaaaggtg
      721 tcccgatccc tgctgcggtg agcgtctttc ccgatgagct ctatgcagcc ccgaggagct
      781 ggacagagag agcctatccc aaactgactc actacaatcg actcccgaaa agcagacact
      841 tcgccgtatg ggaacagctg aacggcgttc aaatcgctgc gttaattgga gcggagcgcg
      901 gcgcgcacac tgacccgatc ggcagagctc ctcaagcgcg tgactatgga gggggccatg
      961 catgaaggcc tcagttaatt caaaagagga aagaagggaa gacaaacatc ttaatcacgc
     1021 taaaccggcg agttgagagt cccgttgcat cttcatgcta taagacgaat aatgtccaag
     1081 atagctttta agttccatta actgctgtat gaggtcggcc tttttcggga gattgtctat
     1141 gcgaaagttt catcccagtg agtcatcgct ttcttcttcc ctcccgacgt tgaagggtag
     1201 aaggtctgat gccctcctcg ccaccgcacg ttctcctcta tatcctttag atccttgcct
     1261 tcgtcatcat atgactgact acgctgtgca accttgaaga cacgactcat tgtgctcccg
     1321 gaccatgaaa tcaggctatc ccataaggag gctcactcat gagtaaagca aagatcttca
     1381 cctattggat tgtgtcgatg ctgatgatgg aactgatggg gactggcgca ggcctcgcta
     1441 ttgcccaacc ctaccaagtg gtcaatgtca cggacggagg caccatcacc ggagtggcga
     1501 catggaaggg cgaggttcct gaacttccac caatcgaaat cgaggcagac acgaacgtat
     1561 gcggcgaaga ggtaccgtct caggcgttac gggtcaattc gaaaaacaac ggcgtgccgt
     1621 acgttcttgt ctatcttgaa ggggttgaga agggaaaggc ccccgcggac aagtactggc
     1681 tccgtatggc gaactgccaa ttccaggagc acgttttccc atttgtccga agccaagtca
     1741 tggcaatgat caaccacgat ccatttttcc acaacccaca ttttttcaac gcaaagaatg
     1801 cctcactcct gaacgtcccc atgcccgatc caagtcaggc aattgagcac aagttcctgc
     1861 ggacacccct caagaacgaa cggggtgtga tgcggcttca gtgtgatgtc cataacaaca
     1921 tgaatgctta ttgggcagca tttgatcacc cgtattttgc ggtcacggac gcagacggaa
     1981 agtttgagat tagcggaatc ccgcccggta ggtacaccgt ggtcgcctgg cacgagggct
     2041 ataaggtcct catcaaggag gcgaccgggc ggccactgta cgatagaccg catattcgcc
     2101 aacaagaaat cgaggtcaaa ccaaaagaga ctgttaaagt cggctttgag ttccccgtcc
     2161 gaaaggtcta tatcgattat tgaggatatg gttgagaaac ctcggagtgg tcgctgcctg
     2221 tgatgacggc ccaccatata taggagaagg tgatgggacg aaggagacta gttctaacga
     2281 gggtgttggt agtggtcctg ctcgcagggc tgcccgtgac ccactccttg gcgaatccgc
     2341 cagaagcgga cgaggtgaaa gatcatccag gcatggagcc gacggttaca cgcggaatgc
     2401 cggatctcgt tcggtattgg aactttgatc cacctaagag tcccttattt gatattggaa
     2461 gagtgacgat ttccggcgac gcacgggtaa ggccagaata ccgcagcaat tcgaactttg
     2521 ggctggcgag cacaagtggt gttggacgtg ctgagggtac gaattcgcaa ggagacaccg
     2581 ctcaattcgt ccaacagtgg gcccggctcg gactcaacta tgcgatgtcg cccgacgtcg
     2641 acaccttctt tcagatgcag tacgcggcga actggggggc gaacagcgcc cctaatacgg
     2701 cgaatgatcc caacacactc acgagcgttg gtgtccgaca ggcctacatc ctgatccgca
     2761 atctcggggt cgatggcctc agcatgaagg caggcagaca gctcatcgtg atgggaaacc
     2821 accgcttgtt cggcaacttt gattggaaca acatcgggtt ctcctttgac ggcatcaccc
     2881 ttcaatattc ccagagaatg tacgaagtct ggggcggctg ggtgcgccta gctgacgcac
     2941 aagccaacgc cggtaactca tcttgcggaa caggcacgac tggctgtgcg ggtggatctg
     3001 gggccgtgaa tcccggcacc gttgccggcg gcaccaccgg gagtaataat gcagatctct
     3061 tctttgcccg gatagccatc aagcccatgg agggcctctc gctcgaaccc ctctgggtat
     3121 ttctcgacaa taatatatcg gtgacagcac cgcctgtcgc taccacctat tatggaacgg
     3181 ccgcgcatgc ccctgggcaa cagcgtcata cgttgggggg gcgc
//
LOCUS       DATIPF010000357         2523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_865242, whole genome shotgun sequence.
ACCESSION   DATIPF010000357 DATIPF010000000
VERSION     DATIPF010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865242"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VLL06_08875"
     CDS             <1..307
                     /locus_tag="VLL06_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HSS31116.1"
                     /translation="PADYFDTRTPRHWMKDHHPALRLAGLVVKNVVGIVFLLAGFAML
                     FLPGQGVLTMLIGISLMDFPKKRELEAKMVGQPTVLGVINSMRQKFGKPPLTLAPGP"
     gene            310..>2523
                     /gene="polA"
                     /locus_tag="VLL06_08880"
     CDS             310..>2523
                     /gene="polA"
                     /locus_tag="VLL06_08880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HSS31117.1"
                     /translation="MPTTESSRKTLYLIDGSAYIYRAFFALPALNNSKGLQTNAILGF
                     TTMLLKILRERKPDGLVVAFDEKGPTLRHAEFKAYKAQRPPMPEGMSAQIPYIHRVVE
                     ALNIPAVRQAGYEADDLIGTLAHQAEQAGFEVVIVTGDKDMFQLLTSHVRIYDPVKDK
                     WLGEAECRERFGVEPARVVEIMGLMGDATDNIPGVKGIGEKTAMKLIAQFGTIEELLR
                     RVEEVTPPRVKTLLIDQAENARLSRRLATIQLDSPVTFDPETYHMKPPHQDQLTDLLR
                     ELGFTTLLKSFQPESKPAETNAAKTELVQDEPSAQRFVDGLPQGHALGLQYLLSSGPG
                     VQAEVQGIALSTGDKTAFIPLDVRTYMRPITTLLHDSARTKIVHDLKATLLVFHRIGL
                     TLAGPYLDTMVADYLLNPNRRDHQLETIAQEVLDHRLETGRKASAAPQSLFDEDTGSR
                     EGTAEAASVLAKLAPVLTERLSEQGSLTLFTDVEMPLVPVLAEIERNGFLLDVEGLHR
                     LSKELERDLDKMIETIGGLAGGEFNINSPKQLATVLFEKLGLKPIRKTKTGYSTDEDT
                     LMQLATQHELPAQIVNYRSLSKLKSTYVDALPELVNPETKRLHTSLNQTVAATGRLSS
                     TEPNLQNIPVKGEYGLRIREAFIAPTGHTLLCADYSQIEPRILAHLSQDPRLLAVFAK
                     GEDIHMATAMEIFSLPSSQITRDMRRVAKTVVFGIVYGISPFGLASNIGVSQAEAK"
BASE COUNT          564 a          730 c          724 g          505 t
ORIGIN      
        1 gccggccgac tattttgaca cgcgcacacc ccgccattgg atgaaagatc atcatccagc
       61 gctacgcctg gcggggttgg tcgtcaagaa cgtcgtcggc attgtcttcc tcttggcagg
      121 ctttgccatg ctcttcttgc ccgggcaggg tgtactgacg atgctgatcg ggatctcgct
      181 catggacttt cccaaaaagc gtgaactgga agccaaaatg gtcggccagc cgaccgtgct
      241 gggcgtcatt aattccatgc gccagaagtt tggcaaacca ccgctgacat tggcccccgg
      301 cccttagcta tgccgactac cgagtccagc cgcaagacgc tctatctgat cgatgggagc
      361 gcctatatct atcgggcatt ctttgccctg ccggccctga ataattccaa aggcctccag
      421 accaacgcca tcctcggttt tacgacgatg ttgttgaaga ttctccgtga gcggaagccg
      481 gacggcctcg tcgtggcgtt tgacgaaaag ggtccaacat tgcgccatgc ggagttcaag
      541 gcctacaagg cgcagcggcc gccgatgccg gaaggtatga gcgcccagat tccttacatc
      601 catcgcgtgg tggaggctct caacattccc gcagttcggc aagccggata cgaggcggac
      661 gatctgatcg gcacgcttgc gcaccaggcc gaacaggccg gcttcgaggt tgtcatcgtc
      721 accggggata aggacatgtt ccaactcctg acgtcccacg tgcgcatcta cgatccggtg
      781 aaagacaaat ggcttggcga agcggaatgc cgcgaacgtt tcggtgtgga gccggcgcgc
      841 gtcgtggaga tcatggggct tatgggcgat gcgacggaca atattcccgg cgttaagggc
      901 atcggtgaga agacagcgat gaagctgatc gcccagttcg gtacgataga agaactctta
      961 cgtcgtgtag aggaggtcac gccgcctcgc gtcaagacac tactgatcga ccaggctgag
     1021 aatgcccgtc tcagccggcg gttggcgacc atccaactgg acagtcccgt gactttcgat
     1081 cccgagacct atcatatgaa gcctcctcat caggatcagc tcaccgattt gttgagagag
     1141 ctgggcttca ccacacttct caaatccttt cagccggaat ctaaaccagc tgagacgaac
     1201 gcagcgaaga ccgagctcgt tcaagatgaa ccctccgcac agcgatttgt ggatggattg
     1261 ccgcagggcc acgctctcgg cctgcagtat ctgctctcct ctgggccagg agtgcaggcg
     1321 gaagtgcagg gaatcgcatt gtctacggga gacaagaccg cgtttattcc actcgacgtc
     1381 cgaacttaca tgcggcctat cacgacgttg ctgcacgact ccgcgagaac aaaaattgtc
     1441 cacgacctga aggcaaccct actggtgttt caccgcatcg gactgaccct cgccggcccc
     1501 tacctggata ccatggtggc ggactatctg ctcaatccca accgccgcga tcaccagctt
     1561 gagaccatcg cgcaggaagt gttggaccat cggcttgaga ccggacgtaa agctagcgct
     1621 gcaccgcaat ccctgtttga cgaggacact gggtcgcgcg aggggacggc agaggctgcc
     1681 tcggtgctgg ccaagcttgc tccggtcctg acggagcgac tgtcggaaca gggaagcctt
     1741 acgcttttca ccgacgttga aatgccgctg gtgccggtgt tggccgagat cgagcggaac
     1801 ggatttttac tggacgtcga aggcctgcac cggctcagca aagaattgga acgagatctc
     1861 gataagatga tcgagacgat cggggggttg gccggtggcg aattcaatat caattcgccg
     1921 aagcagttgg ccaccgtgct cttcgaaaag ttggggctca aaccgatcag gaaaaccaaa
     1981 accggctact cgaccgatga agacacgctg atgcagctcg ccacacaaca cgaattgccc
     2041 gcacagatcg tgaactatcg gagcctcagc aaactcaagt ccacctatgt ggatgcctta
     2101 ccagaattgg tgaatcctga gaccaagcgg ctccatacct cgctcaatca gaccgtcgcg
     2161 gcgaccggac ggctctcctc taccgagccg aatttacaaa atattcccgt gaagggggag
     2221 tacggattac gcatcagaga agcgtttatt gcgccgacag gccacacgct gctctgcgcc
     2281 gactacagcc agatcgagcc gcgcatcctt gcacatctat cgcaagatcc gcgcttgctc
     2341 gccgtgtttg caaaggggga ggacatccac atggcgacgg ccatggagat tttcagcctg
     2401 ccttcaagtc agatcacgag agatatgcga cgggtggcga agaccgtcgt gttcgggatt
     2461 gtgtatggga tcagtccgtt cggtctggcc tcgaacatcg gtgtatcgca ggcggaggcg
     2521 aag
//
LOCUS       DATIPF010000358         6838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1042408, whole genome shotgun sequence.
ACCESSION   DATIPF010000358 DATIPF010000000
VERSION     DATIPF010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042408"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VLL06_08885"
     CDS             <1..317
                     /locus_tag="VLL06_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31118.1"
                     /translation="LKDWIANDAALGPLGSRIAQEASTIPLLDIIADLANCTKHRVLT
                     KTIRAGADLESTSSTVSLHDGVMDRIITIGLEDGSEYTGIVLLDEAHEAWENLLTGHG
                     LL"
     gene            complement(679..960)
                     /locus_tag="VLL06_08890"
     CDS             complement(679..960)
                     /locus_tag="VLL06_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31119.1"
                     /translation="MPHRTICPKCNSDDVKPVTIWRMASANGSVQAPPQPMFACQDLS
                     CLHKWARPSDAATDCTHTVQRLIDLSLVDVGPTMGRYFCEECGTEIVRP"
     gene            complement(1178..1253)
                     /locus_tag="VLL06_08895"
     tRNA            complement(1178..1253)
                     /locus_tag="VLL06_08895"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1218..1220),aa:Met,seq:cat)
     gene            complement(1422..1670)
                     /locus_tag="VLL06_08900"
     CDS             complement(1422..1670)
                     /locus_tag="VLL06_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31120.1"
                     /translation="MGRRPALNVGLFLLGQILLIAGAGYLFSHIPLRVESLLWLILTS
                     VTWFGFLIVYTLIIGVDRAVDLLKDIARKSLPTSNTPT"
     gene            2066..2746
                     /locus_tag="VLL06_08905"
     CDS             2066..2746
                     /locus_tag="VLL06_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31121.1"
                     /translation="MDETTSNHRALEPEPSEYEMPKPCNPHSTTDQQQEQSDQPNRKG
                     ILKNPKYVLNGSIALFAFILIVASIILYLLRSTHQTAERARVTVQKATIVGPLIANNV
                     PRAKILFQNSGRSPALTTKIRLLMTVWTSNKLPDWEMPPILTTDVESVGVIGPGSVVS
                     QDLYLMTPLSEEQGVHLERKDWFIVTLGVVSYIDIFGNPHETKLCLIWRDPSTESLSL
                     CEKWNEAN"
     gene            2867..3436
                     /locus_tag="VLL06_08910"
     CDS             2867..3436
                     /locus_tag="VLL06_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31122.1"
                     /translation="MTHLLPPTCERAILEQFLKAEAMALWAVRSAQAQDVPPAVLSFL
                     RRHEEEEAQHLKQFELLLGTNSHGKTALPRMPSQWKVLAVHLYGYETLGLEFARLLVG
                     LRPDLTSILEDEEVHVGFFEQEVRMILVQGGPAANGARQAAHAWRRRLPRTVDRYLHD
                     ESLAPFCAELRQHILEVIDARFLAVGLTA"
     gene            complement(3541..4956)
                     /gene="sthA"
                     /locus_tag="VLL06_08915"
     CDS             complement(3541..4956)
                     /gene="sthA"
                     /locus_tag="VLL06_08915"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HSS31123.1"
                     /translation="MSTVIHYDIVVIGSGPAGQKAAIQGAKAGKHVAMIEREPGVGGA
                     CVYRGTIPSKTLRESALHMDQMKRSSTTLDRQPLSNTMVSTLMRRLDEVVTSHGDYMM
                     NQLTRNGVTLFHGRAQFRSDSLIEMLTVDGITQTLTADTIVIATGSRPRAPVDIPIDH
                     EHILDSDSILSMIYLPRSLTVLGGGIIASEYASIFAALGVQVTIIDRADRPLQFFDAE
                     LIRTFVQSFEQAGGRYHGAQAIRDVRWDGISQVVTTLQDGQVITGDKMLVALGRQANL
                     EDLNLSATGLTVTEKGTLAVNEHCQTAIPHIYGVGDLIGPPGLASSGMEQGRRAVCHA
                     LGLPEGRSADAIQIGIYTIPEMSSIGLDEAAARKRYREVMVGRSRFNEIARGQISGIQ
                     AGLLKMVADPTGEYILGVQIIGEGATELIHIGQMALQHAATVDSFVETIFNFPTLAET
                     YRVAALDIVGQRQKRLASIAA"
     gene            complement(4953..5822)
                     /locus_tag="VLL06_08920"
     CDS             complement(4953..5822)
                     /locus_tag="VLL06_08920"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HSS31124.1"
                     /translation="MNTDTDHTQNLNIPEQVERALVERIDKDRIDLPVLPQVAGKVIA
                     LANDPSADAARLSALIHQDQALAAHVLRIANSPAYMPRTPIASLQHAVAMLGVNQLSE
                     IAVTISLKSGTVKIPGHEADVRQLWRHALASGAYAKEIARMRRYNVESAYLCGLLHAV
                     GKPVVLKTVTTIAAEMHISLDSSAIIAFLDGYHSRIGNLIATEWALPPQVAEAITYYG
                     IYEQAPSHRLEAMMTCLADRLATYILIPDSFDDSTLREHSVFADLNLYPNDIDALLGL
                     KDKILILVDTMAQ"
     gene            6382..>6838
                     /locus_tag="VLL06_08925"
     CDS             6382..>6838
                     /locus_tag="VLL06_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSS31125.1"
                     /translation="MKPVRVLVVDDHEVTRVGLRTVLSRETSINVVGEATNVKDTVDE
                     ARRLQPDVVLMDVRLSGGSGVDACRAIRDLCPATRVLFLTSYQDDEAVLAAVIGGAAG
                     YLLKHVNAEALVRAIHAVAQGQSILDPAITQPLLTRMRLQKEEPESQRTT"
BASE COUNT         1541 a         1765 c         1946 g         1586 t
ORIGIN      
        1 atctcaaaga ctggatcgcg aatgatgccg ccctcggtcc gctgggttcc aggattgctc
       61 aggaggcgtc gacaattcca ttgcttgata ttattgccga tctcgcgaac tgcacgaagc
      121 accgcgtgct gacaaagacg attcgcgccg gtgcagacct ggaaagcacg agttcgacgg
      181 tcagcttgca tgatggggtc atggacagaa tcatcacgat aggcctggag gatgggtccg
      241 aatatacggg tatagtgctg ctcgatgagg cccacgaggc ctgggagaac ctattaaccg
      301 gacatggcct attatgaacc ctgagcattg acgcaggaac tcacgccact cattacgacg
      361 gccaaaagag gataccgacc agtatcagaa agacaggcgt cagtatgatg agcgcgtctt
      421 gtagcgtgaa acacagcggg taacgcgaaa aatcaaaccg ttccatgatg gggcacctcc
      481 atcaccatac atagtctaat gcttccacac gaggttgtaa cactaggtaa ttacacagca
      541 gtggtcacaa atgagcattt ggatgccctc aggttcatgc gaggggtcac taattcaatg
      601 actgtggagg gagtcaagac gggaggttgc gcacggtcct tcacttactg gcggtgaagg
      661 cattcaccaa aatggcgctt acggtcgaac gatctccgtg ccacactcct cgcagaaata
      721 gcgccccatc gtcgggccaa cgtcgacgag tgacaagtcg atgaggcgtt ggacggtgtg
      781 cgtgcaatcc gtcgccgcat ctgatggtcg cgcccacttg tgcagacagg aaagatcctg
      841 acaggcgaac atcggttgtg gtggtgcttg gacggaacca ttggcggacg ccatccgcca
      901 aatcgtgacc ggtttcacat cgtcgctatt gcacttgggg cagatcgtcc gatgaggcat
      961 ttgccttccc ttactgcaag ggtgagagca ggcatttgcc ttcgcaggac tccttcgcag
     1021 gactgttaat gagtagcaca actcgggagt acgtacaaga ataggaactt ccctagtctg
     1081 ctaagtgcct agctgcagtt tgttacctag gatgttacct gctagccaaa ttcacgtttc
     1141 cgggaacgtg aatatcgctt aaaatgccta tggatattgg tggcggtgcc ccggattgaa
     1201 cgggggaccc gcggcttatg agtccgctgc tctaccaact gagctacacc gccagaatcc
     1261 gcaaaattaa cgacttacga tggtagagtc aagcggggag tgagccattg tgcgtgaatt
     1321 atgctgccaa ccattttgca cgagacagct tggagcacat gaggcgctgc tctgacgaat
     1381 cgccagaaca ctctggtgag catacccatc cggttttcct gtcatgttgg ggtgttactt
     1441 gtgggcagcg atttcctcgc aatgtctttg agcaggtcga ctgcacggtc cacaccgata
     1501 attaaggtat agacgatcaa aaaaccaaac caggtcacag aggttaagat caaccacaat
     1561 agagattcaa cgcggagcgg gatgtgactg aacaggtagc ctgcacccgc aatgagtaaa
     1621 atctggccca ataagaatag ccctacattc agagctggtc ggcgacccat aatggccctc
     1681 cttggtgttg gcatcagata ccgtgcacgc ttgtccttgt ccgattactg taccagttct
     1741 cccactcacc aactatctct ctgtcagggg tgtttccccg cactcattca cccgtgaggg
     1801 ctcacccacc atcgcttatt gtcccatctg cgtgaagggg agactgatgc tcctacggac
     1861 ggcgaatcag tccgctggca ggcatgccac gcttgcgctc cgcgtccagt ttcgatacaa
     1921 cccgtgcctg aataaaggcg tactcggcgc agccatggca ggatgttgag gcgagtcctg
     1981 aaaggctcga tccagcataa actcatccga agaacaagtg cgatgcatcc ataagattgc
     2041 catacttgat tagactgagg gcaacatgga tgaaaccacc agcaatcatc gagcgctcga
     2101 acccgaaccg tccgagtatg aaatgcccaa accatgtaac ccccactcca ccaccgacca
     2161 acagcaagaa caatccgatc agccaaaccg gaagggtatc ctgaaaaatc ccaagtatgt
     2221 gttaaatggt tccatagcgc tctttgcgtt cattctgata gtggcttcca tcattctgta
     2281 tctgctgcga agtacgcatc agaccgctga gcgtgcacgg gtgacggttc agaaggctac
     2341 gatagttggc cccctcatcg cgaataatgt cccaagagcg aaaattctct ttcaaaatag
     2401 tgggcgctct cctgcactga cgacgaagat tcgtctgttg atgaccgtat ggacctccaa
     2461 caagctccct gactgggaga tgcctccgat actgacgact gatgtggaaa gcgttggcgt
     2521 tattggccca ggatctgtgg tgagtcagga cctgtacttg atgacacccc tgagcgagga
     2581 gcagggtgtt catttggagc gcaaagactg gttcattgtg acgcttggag tcgtgtcgta
     2641 catcgatatt ttcggcaatc cgcatgagac gaaactttgc ctgatatggc gagatccctc
     2701 caccgaaagt ctgagtctct gtgagaaatg gaatgaagcc aattaatatg gctaccaaca
     2761 ccgcgccgag tatgcctttg ctccgtgcgc gtaccaagaa tcaagtcccg gataatcgcc
     2821 ctactcagat gttggttccg ccccgtggtt tcgatagact gggtatatga cgcatctgct
     2881 tcctccgacc tgtgaacggg cgatcttgga acaattcctc aaggcggagg ccatggcctt
     2941 atgggcggta cgttctgcgc aagctcagga cgttcctccc gcggtgttgt cgttcttgcg
     3001 tcgtcatgag gaagaagaag cgcagcatct caagcagttc gagctgctgt tggggacgaa
     3061 ttcgcacggc aagacggcgc ttcctcgcat gcccagtcaa tggaaggttc tcgcggtgca
     3121 tctctatgga tacgaaacgt tgggattgga gttcgcgagg ttgttagtcg gtttgcggcc
     3181 tgatctgacg tcaatattgg aagatgaaga agtgcacgtg ggatttttcg aacaggaagt
     3241 tcgtatgatc cttgttcagg gcggacctgc cgcgaacggt gcgcgacaag cggcccatgc
     3301 ctggaggcgg cgcctgcctc gcacggtcga tcgctatctt cacgatgaaa gtctcgcacc
     3361 gttttgtgct gaattgcggc aacatattct cgaggtcatc gatgcacgct ttcttgccgt
     3421 cggattgacg gcatgatcgg tgaaacaggg tcggtctctg gtgaagatgg caagaggcga
     3481 ggcaggcgcg tctgacagcg attacttctt acttcgacct ggtatcccgc cgctcacgtc
     3541 tcatgcggcg attgaggcta gacgtttttg tcgttgtccc acgatatcaa gtgcggcgac
     3601 gcgataggtt tcagccaacg tcggaaagtt gaagatggtc tcaacaaatg aatcgacggt
     3661 ggccgcatgc tgaagcgcca tctggcctat atgtatcagt tccgttgcgc cttcaccgat
     3721 aatctggaca ccaaggatgt attcgcctgt cggatcagcg accatcttca gcaatccagc
     3781 ctgaatgcct gaaatctgac cgcgagcgat ttcgttgaac ctcgaccggc cgaccatgac
     3841 ctctcggtat cgtttccgag ctgctgcttc gtccagtccg atactggaca tctcggggat
     3901 ggtgtagatc ccgatctgaa tggcatcggc tgaacgtcct tccgggaggc ccaaggcgtg
     3961 gcacacagca cgccggcctt gttccatacc agaagaggcc agaccagggg ggccaatgag
     4021 atcgccgacg ccatagatgt ggggaatagc ggtctggcaa tgctcgttga cggccaacgt
     4081 tcccttttcg gtgacggtca ggccggtggc gctgaggttg aggtcctcca gatttgcctg
     4141 tcgccccaag gccacgagca tcttgtcgcc ggtgatcact tgtccgtcct ggagcgtcgt
     4201 caccacctga gagatgccgt cccatcggac gtctcgtatc gcctgcgccc catgataacg
     4261 gccgcctgcc tgttcaaagc tttgcacgaa tgttcgaatg agttccgcat caaaaaactg
     4321 taggggacga tcggcccgat cgataatcgt gacctgcacg ccgagtgcgg caaagatcga
     4381 tgcatattcc gatgcaatga tgcccccgcc taatacggta agggagcgtg gcagatagat
     4441 catggagaga attgagtcgc tgtcgagaat atgttcatgg tcgattggaa tgtcgaccgg
     4501 ggcacggggt cttgaccctg tggcgatgac gatcgtgtcg gcggtgaggg tttgtgtgat
     4561 gccgtcgacc gtcaacattt caatcaggct gtcggagcga aattgggctc ggccgtggaa
     4621 caatgtgacg ccgttgcgtg tcagctggtt catcatatag tcgccatgtg atgtgacgac
     4681 ctcatccaag cggcgcatca gcgtggagac catcgtgttc gaaaggggtt gtctgtccag
     4741 cgtggttgag gaccgcttca tctggtccat gtggagcgca ctttcgcgga gcgttttgct
     4801 tggaattgtt ccccgataca cacaggcgcc tccgacacca ggctcgcgtt cgatcatcgc
     4861 cacatgtttg cctgctttgg cgccttggat ggcggccttt tggccggcgg gaccgctccc
     4921 gatcaccaca atgtcatagt gaatgactgt actcattgcg ccatcgtgtc cacgagaatc
     4981 aggatcttgt ctttgaggcc aagtaatgcg tcaatatcgt tggggtagag attgagatcg
     5041 gcaaagacac tgtgttcacg aagcgtgctg tcatcgaagg aatcgggaat gaggatgtag
     5101 gttgccaatc ggtcggccag acaggtcatc atcgcttcca gtcgatgcga gggtgcctgc
     5161 tcataaatcc catagtaggt gatggcctca gccacctgtg gaggcaaggc ccattcagtc
     5221 gcgattaagt tgcctatgcg ggaatggtac ccgtcgagga aggcgatgat cgcactggaa
     5281 tcgagagaga tatgcatttc agcggcaatg gttgtgacag tcttgaggac gactggcttt
     5341 cccacggcat gcagaagtcc acacaggtag gcgctttcca cattgtatcg gcgcatgcgc
     5401 gcaatttcct tggcataggc cccgctggcc agtgcatggc gccagagttg ccgtacgtcc
     5461 gcttcgtgtc caggaatctt gacggtcccg cttttcagtg aaatcgtgac ggcgatctcc
     5521 gacagctgat tcacaccgag catggcgacg gcatgttgaa gcgaggcaat cggcgtccga
     5581 ggcatatagg caggcgagtt ggcaattctc aggacatgag ctgccaaggc ctggtcttgg
     5641 tgaataaggg ctgacagacg ggctgcatct gccgatggat cattcgcgag ggctatgacc
     5701 ttgccggcca cttgtggaag tacggggaga tcgattcgat ccttgtcgat ccgttcgact
     5761 aaggctcgtt cgacctgttc cggtatgttg agattctgtg tgtgatcggt atcggtattc
     5821 atgccgcagg tagttcttga gagcggttag ggtccactgc atgtcagtgc aatctcttgt
     5881 cggtcgacat ggttgggaac tttaatccat ggacctgggt gtcgggtcgt ggaatctgtg
     5941 caatcacaaa ggccttggtg agagtatgcc ggtcagaggg tggcctgtat tgtgtgtgcg
     6001 attgttggta cgtataaata tacgtagtct atatgatgtc agaattttat acactaatat
     6061 ttcgattatg aatttcatgt cagcattgtt agagccgatt agcattattg ctattaaata
     6121 gagaacagat gtaaatttca gcgcaatgat gcggtagtcg aaatgtgagg ctgcccatgc
     6181 atgacggttc tcgcctcccc ttcttataca tgaccgagcg cagtacctcc atatctggtc
     6241 tggcaggact caaacgggct cgaacgttgc tgggaatcca gcccatacaa aatagccatg
     6301 gcaggtttgc actccgtgct ctcgaccggc tcagagggta ttcacttaac cacccccacg
     6361 gaggaggcac ttttgtctaa catgaaaccg gtcagagtgt tggtcgtgga tgaccacgaa
     6421 gtcacgcggg tagggctgcg gacggtgctg agtcgagaga ccagcatcaa cgttgtcggc
     6481 gaagccacga acgtgaagga cactgtggat gaagcccgcc gccttcaacc tgatgtcgtc
     6541 ttgatggatg tccgcttgtc cggcggcagc ggagtggacg cgtgtcgtgc catcagggat
     6601 ctctgtcccg cgacgcgtgt gctctttctt acctcctatc aggacgatga ggcggtgctc
     6661 gctgcggtaa tcggaggggc ggccggttac ttattgaaac atgtgaatgc cgaggcgttg
     6721 gtgcgagcca tccatgctgt ggcacaaggg cagtcgatcc ttgatccggc cattacgcag
     6781 cctctgttga cccgcatgcg gttgcagaaa gaggaacccg aatctcaacg gacgactc
//
LOCUS       DATIPF010000359         5286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1042727, whole genome shotgun sequence.
ACCESSION   DATIPF010000359 DATIPF010000000
VERSION     DATIPF010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042727"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1706)
                     /locus_tag="VLL06_08930"
     CDS             complement(90..1706)
                     /locus_tag="VLL06_08930"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HSS31126.1"
                     /translation="MKRQLMGQIISHTNRTFLWRILFLLCMGLLVHANPSQAESKASV
                     DSLLVKGFSFSGNTVVSQSELEALTQPYVGRSLTLSDLEEVAATVAERYKQKGYTLAT
                     TYVPQQQIRFGVVTIAILEGRVGEISVTGNQHYSTKFIRGSFAQAMEDNVVRNVALER
                     ALLLLNEYPNLKVSAVLEPGASTGSTNVHVKAEDKLPVHASLDYNNYGANTISRNRFG
                     AGIEVGNVLFDGATLNFNGILGDHPDRLLFFTGAYAVPIGVHGTKLVLSGSDGRFDVG
                     AQLAALQIHGSIKTYDISVTHPYIKTRFQTLIAEAGFASKDNRLFILGNLTSDDRVRM
                     AKLGVNYDRLDLSGRTYFSLYGFQGLGEALGGMDNNTLRTTRQGSDNRFTKATLNTGR
                     IQSLGHDVLLVVRANAQATTGPVVVIEQMLLGGPDSVRGYQLGERFVDEGYTVSAETR
                     VPFFPSVLGSTQAAVFIDHGAGRLRNPQPGEHASSSLTGTGVGLQTELPYLSTRLRMD
                     IGFPIGPTPAGGTVTGDRSPIFYLQAIARF"
     gene            2110..2688
                     /gene="pyrE"
                     /locus_tag="VLL06_08935"
     CDS             2110..2688
                     /gene="pyrE"
                     /locus_tag="VLL06_08935"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HSS31127.1"
                     /translation="MTVRDDLSRAFRETQSFKWDPIKKFTLASGETSPFYVDCRSLLA
                     YPESRRLVGHLAHDALVGVTIDCLGGLEIGAISIATTISDYCYRATPRRDWRTFVVRK
                     TAKDHGLGKLIEGAVRAGDRALIVDDVLTSGGSLLKAIAAARQAGLVVSEALVIVDRK
                     EQDGRARVEQEGVSLISLLTIDDLTRQQVETP"
     gene            complement(2772..3077)
                     /locus_tag="VLL06_08940"
     CDS             complement(2772..3077)
                     /locus_tag="VLL06_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31128.1"
                     /translation="MKSWSSLICALLLLLAGCGEGAKLVQDTGDGGVTTYPFKGEQGY
                     LLSSFRQEALALMRDKCGRSYTIVREGEAKGRARVVGVEGSQEIVQERRWGIEFRCK"
     gene            3153..3578
                     /locus_tag="VLL06_08945"
     CDS             3153..3578
                     /locus_tag="VLL06_08945"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSS31129.1"
                     /translation="MNTRIAMIIAGLCMTLIATVRLVSADHSYLLTVQQLEAGLTKAP
                     SSTQKGFVLIDVRSPEEHAQGFIPGTDFNIDFREIVARHREFAAELDDHIVVYCQSGR
                     RSNIAAETLADLGYRHVYNVSGSMNAWLAAGFPVESGRR"
     gene            complement(3753..4640)
                     /locus_tag="VLL06_08950"
     CDS             complement(3753..4640)
                     /locus_tag="VLL06_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31130.1"
                     /translation="MMVQDNSVQWSFPVAQCLLGLIIVVFSGPSTTMAQTLAEDQLGD
                     GLSISVWNPRTQCPDVAPLVAVEIDPDRYRFTVHYYQQDGLSDPLDIRQWQERTGHDL
                     VFNAGLFRENYAYLGLLYGNGRSLGSKRHVTWLGLFVAEPAEHHSRRARVLDLSYDAF
                     DERQPPYREVAQSLMLLDRTGKIRVRQTGKRAHQTLVAEQGNGHLVILKTTEVVSLYA
                     LGECLRDAFPSLRQVMAMDGGSSSDVAVSPSLRQAMSRAAGVHTGIPLLEGITTAHVG
                     LPAVIGISPRRDHPDDKLR"
     gene            complement(4729..>5286)
                     /locus_tag="VLL06_08955"
     CDS             complement(4729..>5286)
                     /locus_tag="VLL06_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS31131.1"
                     /translation="QCGGAIDVTSRVGHGTRFDLYFPNVESDILTTASTQPAGQPQRG
                     TETILLVEDDPSVRTLVRDELRKLGYRVLEAKNGVEACLLATQQTGLVQLLLTDVVMP
                     GMGGRELAQHLSVIKPDLRTLFISGYMDDIGIMAGQEEGTSSFLQKPFTPEVLARAVR
                     NLLDVSTPSARTGASSSSTPQNTPC"
BASE COUNT         1152 a         1458 c         1502 g         1174 t
ORIGIN      
        1 cggggaaaac ccctgggttt taacgggtta caactgctct tatctgccgt cattggttca
       61 ctgtaggtat ctgccgtcat gcatctcgtt cagaaccgcg cgatggcttg caaatagaaa
      121 atcggggagc gatcgcctgt cacagttcct cctgccggcg taggcccgat aggaaaccca
      181 atatccatgc ggagcctcgt cgaaagatac gggagttcag tttgcagccc gaccccggtg
      241 ccggtcaggc tgcttgaggc atgttcgccc ggttgaggat tacgcaaccg tcccgctcca
      301 tggtcgatga agactgccgc ttgcgtcgaa ccgagcacag agggaaagaa cgggacgcgc
      361 gtttctgccg acaccgtgta gccctcatcc acaaaccgtt cgccgagctg atagcctcgc
      421 acagaatccg gtccacccaa gagcatctgt tcgatgacca cgaccggccc ggtcgtggct
      481 tgcgcgttcg ctcgtacgac cagcagcacg tcgtggccga ggctctgaat acggccggta
      541 ttcagcgtgg ctttggtgaa acgattatcg ctcccctgtc tggtggtccg aagggtattg
      601 ttatccatgc cgcccagtgc ctcccctaat ccttgaaatc catacaacga aaagtatgtg
      661 cgccctgata gatcgaggcg atcgtaattc acacccagtt tggccatgcg gactcggtcg
      721 tcactggtca gattgcccag gataaagagc cgattgtcct tcgaggcaaa tcccgcttcg
      781 gctatcaacg tctgaaatcg cgtcttgata taggggtgag tgacagaaat atcgtatgtc
      841 ttgatgctgc catgaatctg gagggctgcc agttgggcgc cgacatcgaa tcgtccgtcg
      901 gatccggaca gtaccagctt cgtaccgtgg acaccgatag ggacggcata ggcaccggtg
      961 aaaaacaaca gacggtcagg gtggtctccc agaatcccat tgaagttgag cgtcgcgccg
     1021 tcgaagagca cgttcccgac ttctatgcct gcaccgaacc ggttccgcga gatcgtgttg
     1081 gcgccgtaat tgttgtagtc cagcgacgca tgaaccggca gtttgtcctc agctttgaca
     1141 tggacattgg ttgagccggt tgaggcgccc ggttccaaca cggcagagac tttcaggttg
     1201 ggatactcgt tgagcaagag caaggcacgc tccaaggcca cgttccgaac gacgttgtcc
     1261 tccatggcct gagcgaaact gccacgaatg aatttggtcg aatagtgctg attgccggtg
     1321 acggagatct ctccgacccg gccttccaag atcgcaatgg tgaccacgcc aaatcggatt
     1381 tgttgttgcg gtacataggt ggtcgcgagg gtgtacccct tctgcttgta gcgctccgcc
     1441 acagtcgcag cgacctcttc gaggtcggac agagtgaggg agcgcccgac atagggttga
     1501 gtaagcgcct ccagctcaga ctgagagacg acggtgtttc cgctgaagga aaatcccttc
     1561 acgagcaggc tgtcaacaga ggccttcgat tctgcctggc tcggattggc atggactaag
     1621 agacccatac agaggagaaa gagtatgcgc cagaggaatg ttcgattcgt gtgactgatg
     1681 atctgtccca tgagttgtcg cttcactttg ctctcgagct atgccaagat aaccgcggcg
     1741 tgaagcgagc caacacatga ggtcgcaggc ttgttgaagt ggatcttgct catgagtctc
     1801 gtgggagtct ggtccggctg tgctatctac ccggatcctt cctcggaacc tcgcacgaag
     1861 acggattcgg tcgagaacca gcctcctccg gagattgaaa agacagaacc gccgaagcga
     1921 ccttccacca catgcccatc agcaatgcga ggatcttgct agcagtgctg ttgccagcaa
     1981 gattcgtgct actagggtca tttgaccgta acgtgaaagg aagagctagg caatgaaata
     2041 tctgtttttc tacgtaagac cttcagggca cacgcttcat ctttctgctg ctcccacgga
     2101 caatcgagca tgacagttcg agacgacctc tcgcgcgcat ttcgtgagac acagtcgttc
     2161 aagtgggacc cgattaaaaa gtttacgctg gcgtccggtg agaccagtcc cttctatgtc
     2221 gattgtcgat cgctcctggc ctatcctgag tcgcgccggc tcgtgggaca tctggcacat
     2281 gacgcattgg tcggagtgac catcgactgt cttggagggc tcgaaatcgg cgcgatatca
     2341 attgccacga ccatttctga ctattgctac cgtgcgactc cacgacggga ctggcggacc
     2401 ttcgtggtgc ggaagaccgc gaaggaccat ggattgggga agctgattga aggggccgtg
     2461 cgtgcgggag atcgcgcgtt gattgtcgac gatgtgttga caagcggtgg gtccctgctg
     2521 aaagcgattg ccgcggctcg gcaagccggc cttgtggtaa gtgaagccct cgtgatcgta
     2581 gatcggaaag aacaagatgg gcgcgcacgc gtcgaacagg aaggagtgtc gttaatcagt
     2641 cttctgacga tcgatgatct gacacggcag caggttgaaa cgccttagca ggatgctgga
     2701 acactatctt ggcgtgtgac gcttcggtac gttcgcccgt ctggctcaac aggagtacac
     2761 attcgcagca ctcatttgca ccgaaactca atcccccacc gccgctcctg cacgatttct
     2821 tgagatcctt caacaccgac gacacgtgcc cttcccttgg cttccccttc ccgcacgatt
     2881 gtataactgc ggccgcattt atcccgcatc aaggcgagag cttcctgccg aaacgaggaa
     2941 aggagatacc cctgctcccc cttgaagggg tacgtcgtca cccctccatc gcctgtgtcc
     3001 tgcacaagct tcgcaccctc gccgcagcca gccaggagca ggaggagcgc acatatgagg
     3061 cttgaccaac tcttcatacg actcctatca taataggcag aatcgttctc tatcgaatcg
     3121 cattcatcat cgctgtaatc aggagcccca tcatgaatac acgaattgcc atgatcattg
     3181 ccggcctgtg tatgacgctc atcgcgacag tgaggttggt atcggccgac cactcatatc
     3241 tgttgacggt ccagcaactg gaggctggat tgacgaaagc cccttcgagt acccagaagg
     3301 gttttgtgct gatcgatgtg cgctctcctg aagaacatgc gcaaggcttc atccccggaa
     3361 ccgatttcaa catcgatttc cgtgaaattg tggcgcgcca ccgtgagttc gccgccgaat
     3421 tggacgacca cattgtcgtc tattgccaat ccggtcgtcg cagcaatatc gccgcagaaa
     3481 cgttggccga tttaggttat cgccatgtct acaacgtgag cggcagtatg aatgcctggc
     3541 ttgcagccgg ctttccggtt gaatcaggcc gccgctaaag gatattgggg aaagtccacc
     3601 aacaccacgc atcatcctgc tagtctcatg aggctattct gggtggatgt ttgcctcatc
     3661 tccctgacgt atggatcatc gaggatcttc cgaatcgaaa aggttttctc caaccagctc
     3721 gttcgacatg catcgcacca cgctcatctg gtctaccgga gtttgtcgtc tggatggtcg
     3781 cggcgcgggc tgattccaat cacggctggc aatccgacgt gagcggtggt gatgccttct
     3841 aacagtggta tcccggtgtg cacaccggcc gccctagaca tggcttgtcg gagcgaggga
     3901 ctgacggcga cgtcggaaga ggatcctcca tccatggcca tgacctggcg tagagaggga
     3961 aaggcatccc gcagacattc tccaagggca tagagtgaca ccacttccgt ggtcttaagg
     4021 ataacgagat gtccattgcc ttgttctgcg acgagggtct gatgtgcgcg tttcccggtt
     4081 tgccgcacac ggatttttcc tgtccggtcc aggagcatga gggattgagc cacctctcga
     4141 tagggaggtt gtcgctcatc gaaggcgtca tagctcaagt ccaagacccg cgcgcggcgt
     4201 gagtgatgtt ctgccggttc tgccacaaac aatcccagcc acgtgacgtg cctcttgctc
     4261 cccaacgagc ggccgtttcc atagagcaag ccgagatacg cgtaattctc acggaaaaga
     4321 ccggcgttga ataccagatc gtggccggtt cgctcttgcc actgacgaat atcgagcgga
     4381 tctgataatc catcctgctg gtaatagtgt acggtaaagc gatagcgatc gggatcgatt
     4441 tcgacggcga ccaacggtgc gacatcaggg cattgcgtgc gaggattcca gacagaaatg
     4501 gataaaccgt ctccgagttg atcttctgcg agggtctgag ccatcgttgt cgaaggtccg
     4561 ctgaacacga cgatgatgag acccaggaga cattgggcga cagggaaaga ccattggacg
     4621 ctgttatcct gtaccatcat atttcaggat caagtataga gagggccggt acggagcgtc
     4681 aaatcctacg aggaagtgag gcaacggctg cgaggtgagc ctcgcaggtc agcatggagt
     4741 gttttgaggt gtcgatgaag aggacgcacc ggtcctggct gatggcgtgc tgacatcaag
     4801 caaattgcgg acggctcggg cgagcacctc gggagtgaag ggtttttgta agaaactgct
     4861 cgtgccctct tcttgtccgg ccatgatgcc gatgtcatcc atgtagccag atatgaacag
     4921 agttcgtaaa tcaggcttga tgacggagag atgctgtgcc agctcccgtc cgcccatgcc
     4981 cggcatgacg acatcggtca gaaggagctg gaccaatccg gtttgttgcg tcgccagcaa
     5041 gcaagcttcg accccatttt tggcttctag aacccggtag cccagcttcc gcagttcatc
     5101 tcgcacgagc gtacggacac ttgggtcatc ttccaccaag agaatcgttt ccgtgcctct
     5161 ctgcggttgt cccgcgggct gggtcgacgc tgtggtgaga atatcggact cgacgttcgg
     5221 gaaatacagg tcgaagcgtg tgccgtgtcc cactcgactg gtcacgtcga tggcaccgcc
     5281 gcattg
//
LOCUS       DATIPF010000360         6729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_337873, whole genome shotgun sequence.
ACCESSION   DATIPF010000360 DATIPF010000000
VERSION     DATIPF010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337873"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VLL06_08960"
     CDS             complement(<1..381)
                     /locus_tag="VLL06_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HSS31132.1"
                     /translation="MIRAMCTAATGMTAQQINVDTIAHNLANVNTSAFKRSRAEFADL
                     LYQVQRMPGTNASNIGVFPVGIQIGAGVRPVTVAKEWQQGNMRQTSNDLDLAIDGPGF
                     FQVTRPDGTAMYTRNGSFKRDNVGN"
     gene            complement(400..1170)
                     /locus_tag="VLL06_08965"
     CDS             complement(400..1170)
                     /locus_tag="VLL06_08965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HSS31133.1"
                     /translation="MNRAIYPILSGALAQERQMQVFANNMANVNTAGFKQDAQAFKSV
                     MAQVQVGAPVFAHTAGFGHQIGVRPAGPTERVFTAPHALRTSFEAGRIRITGNSLDAA
                     IQGSGFFEVKTPQGIRYTRSGMFSLDAQRRLVTNLGYPVMGTKGEIKIPPGSAQINAQ
                     GAIHVDDKPVANLKIMDFPMDDMPEKHLDGLFASDSAQPAKNPQVQGGHIEESNVNSI
                     GEMVKMIQSTRSYESAQKLIQTLDHMADVSIQSVGRVL"
     gene            complement(1425..2078)
                     /locus_tag="VLL06_08970"
     CDS             complement(1425..2078)
                     /locus_tag="VLL06_08970"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HSS31134.1"
                     /translation="MGLLVLGGLWLKGWYQNSGFRRKRSGEKSTELSSTYDHVTGLPT
                     SRLFGTLLDQSIGRAAKIGRPVALLVVELEHFRMVAESQGHANGNILVRVQAARVKGV
                     LRSTETVARLAQDQLAMILDNLTSIQDVSAIVEKLQATIGLPLTLDGHELFLTCRIGV
                     ALYPYDATEQEELIRKAMQAVKTAKSEGQAVQFASPIVAVPSSEPVSVPSSFAARLP"
     gene            2077..2241
                     /locus_tag="VLL06_08975"
     CDS             2077..2241
                     /locus_tag="VLL06_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31135.1"
                     /translation="MRQTPMNAPMGDITTLGSPALAVQPFSKGMEASLYMERLIVLRI
                     VISRDRSMEY"
     gene            complement(2404..3153)
                     /locus_tag="VLL06_08980"
     CDS             complement(2404..3153)
                     /locus_tag="VLL06_08980"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HSS31136.1"
                     /translation="MSNIATQHKRQIGPASETHRELIIKEFAHVVKAMAYRLAYRLPA
                     YMDAEDLVSVGIIGLMDAMDKYDPSREAKFKTYAEFRIRGAMLDEIRSMDWIPRSVHE
                     RVTVLQRAHTKLLNRLGRPPTDEEVATEMKLSPAELDEFLVRSRGAVLVSLDDINLRE
                     PDGPKILDMLADTHHPDPLAIILNDQERERVASAIQQLPEKERLVLTMYYYEELTMKE
                     IGRIFKVTESRVCQIHTKAVLHLKSKLEALS"
     gene            complement(3201..4058)
                     /locus_tag="VLL06_08985"
     CDS             complement(3201..4058)
                     /locus_tag="VLL06_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HSS31137.1"
                     /translation="MKMFSEQETATPTMDHGNPCTRVLTVTSGKGGVGKTNIVANLAV
                     ELARVGKRVLVLDADLGLGNIDVLLGLVPRHTLEDVLCGSRHLSDILLQGPAGIQVLP
                     AGSGLPHLTSLTDSQQLILQTELEHVANAVDVLLIDTGAGVSPNVTYFASAAQEIIVV
                     ISPEPTSLTDAYALMKVLCRQHRERRFKVLVNMVKSQREAMQTFRKLDVAAERFLSIR
                     LEYMGFIPLDDYLPMAVIEQKAVTERFPCSPASRAFVQVAKTVAEWPDPQLPKSTVQF
                     LWQQLIPMS"
     gene            complement(4073..5428)
                     /gene="flhF"
                     /locus_tag="VLL06_08990"
     CDS             complement(4073..5428)
                     /gene="flhF"
                     /locus_tag="VLL06_08990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HSS31138.1"
                     /translation="MKMKVFHADTMHDAIRAIKEELGPDALILSTKRVRRGSLPFSLF
                     GRPLLEVTAAIDASADQAPSVRQAASAIAQDEVHASESQREQAPAFQDTLSALLHYKK
                     HTPSESGDTNVRNPMPTPKPESRPVPQGRFRHLKNELLDLQQLLTSSLPDEVQTAGSG
                     LPAPLARACRLLVAQGLRPSTAESLCLTLRRDLSQEALRSDREIQDVLYRLCTEQIRV
                     SGPLLDGANDYKVALFVGPSGAGKTTTIAKLAAHYRLQEGRSVALITLDTCRLAAVEH
                     LRMYAKVLGLPLETAQTTAEVVEGIRRHRQASLIMIDTPGFGSHESAQLMNLGQLKDS
                     CESIDTHLVLSATTRMQDLRRTVARYGACTPSRLLFTKLDETEEYGNLFELAYQTTLP
                     LSYWSNGQRVPEDVELADAARLADLLLDRGSARFTSSGLSSSIGRISAVSMGGTTPQH
                     A"
     gene            complement(5437..>6729)
                     /locus_tag="VLL06_08995"
     CDS             complement(5437..>6729)
                     /locus_tag="VLL06_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HSS31139.1"
                     /translation="AATDADLGHQITGQMLLSPKAVGTAAGILLALGLVPGLPHLAFL
                     GLGGAAAWLAYRLSQEAKAPSEPAPVSVAPQSESTTPQLAPLDLMEVQVGYGLIGLVE
                     GKHGTALLDRIKGLRKQFADTMGFLLPPIHIRDNLQLRPNEYAVMLKGVELTKADVLP
                     SHVLAIDPGTAQRGLIHGVATKEPAFGLPALWVPESTREQAQLAGYTVVDASSAIATH
                     LSEIIKRHGHELLGRQEVQSLLDELAKAHPKLVEELIPTLLPLGAVVRVLGNLLHEGI
                     PIRDLRSIIEALADHASTTKDPELLTEMARQALGRTITRQYQAPDGTLPVITLDPRLD
                     RSLSEQAASVPQGGMLNLDPGLSHTLLAAMKQAVERVAARGHQPVLLCSPALRRHIRR
                     LTDRILHAVPVVGFNELDSMVRLQSLDTIRLDGDALLP"
BASE COUNT         1427 a         1962 c         1892 g         1448 t
ORIGIN      
        1 atttccgacg ttgtcacgct tgaaggatcc gttgcgggta tacatggccg ttccatctgg
       61 ccgtgtcacc tggaaaaacc cggggccatc gatcgccagg tccaagtcat tgctcgtctg
      121 ccgcatgttt ccctgctgcc attccttggc gacggtcaca ggacgaaccc ctgccccgat
      181 ctgaataccg acgggaaaaa caccgatatt cgacgcgttg gtgcccggca tacgttggac
      241 ctgatacagc aaatcggcga actccgcgcg gctgcgcttg aacgcgctcg tattgacgtt
      301 cgccagattg tgggcaatcg tatcgacgtt gatctgttgc gcggtcattc cggtggccgc
      361 cgtacacatc gctcgaatca tacgaaacct cctacgtcct tagagcactc gaccgacact
      421 ttggatcgag acgtctgcca tatgatccag tgtctgaatc agtttctgcg ccgactcata
      481 gctcctcgta ctctggatca tcttgaccat ctcaccgatt gaattgacgt tcgactcctc
      541 gatatgcccc ccctggactt gtgggttctt cgccggttgg gcggaatccg aggcaaacag
      601 tccatccagg tgcttctccg gcatgtcatc catgggaaaa tccataatct tgaggttcgc
      661 caccggttta tcatcaacat gaatcgcccc ttgcgcattt atctgggccg agccgggcgg
      721 aatcttgatt tctccttttg tgcccatcac cggataaccc agattcgtga ccaaccggcg
      781 ctgagcatcc agcgagaaca tgccgctcct ggtatagcga atcccctgcg gagtcttgac
      841 ctcgaaaaat ccgcttcctt ggatcgccgc atcgagagag ttcccggtga ttctgatccg
      901 gcctgcctca aatgacgtgc ggagagcatg gggggctgta aagacccgct ctgtcgggcc
      961 ggccggtcgg acgccgatct gatgaccgaa accggcagta tgcgcaaaga ccggcgcgcc
     1021 aacctgtacc tgcgccatga cagacttaaa cgcctgggcg tcctgcttaa atcctgctgt
     1081 attcacattt gccatgttat tggcgaagac ctgcatctga cgttcttgcg ccagcgcacc
     1141 ggacaggatg ggatagatcg ctcggttcat gtgtgctctt tcatgttgga ctgttcggcc
     1201 aagtcacttc gcaacatgca tgccatctgt ctccaaccgt agtgcaatga gtcgcgcgaa
     1261 tggcgagcca ggaagcaggc tgtaaaaaca gtatgatggc gcaaatcaca ttcatgtgaa
     1321 tcaggtggca ccgagagcga catgcggaag gaataggtcg cactaggaaa agattgcctg
     1381 aggggaagaa agttacgggt tgagtgcgaa cgaataaatg gggattacgg gagacgggct
     1441 gcgaaggaag agggcacgga taccggttca gaggagggca ctgcgacgat aggtgaggca
     1501 aattgaacgg cttgtccttc agacttcgcc gtcttcaccg cctgcatggc ctttctgatt
     1561 aactcctcct gttccgtcgc atcgtacgga tacagtgcca caccaatacg gcatgtcagg
     1621 aataattcat gtccatccaa ggtgagagga agccctatcg tcgcctgcaa tttttccaca
     1681 atggccgaca cgtcctgaat ggaggtgaga ttgtcgagaa tcatcgcgag ttgatcctgc
     1741 gccagccgcg caaccgtttc cgtggaacgc aagacgccct tgaccctggc tgcctggaca
     1801 cgcacgagga tattaccatt cgcgtgcccc tgactctctg ccaccatccg gaaatgttcc
     1861 agctccacca cgagcagcgc gacgggtcga ccgatttttg ctgcacgccc tatcgattga
     1921 tcgaggagtg tcccgaataa tcgactagtg ggcaatccag tcacgtgatc ataggtggat
     1981 gagagctccg tggatttttc gccagaccgt tttctacgga atccggaatt ctgataccag
     2041 cccttcagcc acagtccccc gagaacaagg agccccatga ggcagacccc catgaatgcc
     2101 ccgatgggcg acatcaccac actcggaagt cccgcgcttg cagtccaacc gttcagcaaa
     2161 ggtatggagg cgtcactgta tatggagagg ctcatcgtat tgaggatcgt gatttcccgc
     2221 gaccgatcaa tggaatacta accatggagt gtgtcggtcg atttcaggtc agacttgagc
     2281 ggagctggac ggatagaaac cggcattagc ctggcagaac ggatatggag ccggtgcgat
     2341 gcctcacgcc gagcctctgg cgggacagcc ggtgagctgg cgcaatgcgc tatgccacgc
     2401 ggttcatgac agcgcttcga gcttgctctt gaggtgaaga accgctttgg tatggatctg
     2461 acacacgcga gactccgtga ccttaaagat tcgcccgatt tccttcatcg tcaattcttc
     2521 atagtaatac atggtgagca cgagccgttc cttctccggc aattgctgaa tcgcactggc
     2581 gacccgctca cgctcttgat cgttgaggat aatcgccagg gggtcgggat ggtgcgtgtc
     2641 ggcaagcatg tcaagaattt ttggtccgtc cggctcacgc aagttgatgt cgtccaagct
     2701 gaccagcacg gcaccccgtg accgcacaag aaattcatcc agttcggctg gagacagctt
     2761 catctctgtc gcaacttctt catctgtcgg aggacggccg agcctgttca acagtttggt
     2821 atgcgcccgt tgcaacaccg tgacgcgctc gtgaaccgac cggggaatcc aatccatgga
     2881 gcggatttcg tccaacatgg cgccccgaat acgaaactcc gcataggttt tgaacttcgc
     2941 ttcacgactg gggtcgtact tatccatcgc atccatcagc ccaatgatcc cgaccgacac
     3001 caggtcctcc gcatccatat aggctgggag acggtaggcc agccgatagg ccatcgcctt
     3061 caccacatgt gcgaactctt tgatgatcag ttcgcgatgc gtctcggacg cagggccaat
     3121 ctgccgttta tgttgcgtcg ctatattgct cattatccgt gcgccttctg tttctgtatc
     3181 catatcgtca ggacgctttc ctaggacatg gggatcaatt gctgccatag aaactgcacg
     3241 gtactcttcg gcagttgagg atcgggccat tcagcaaccg ttttcgccac ctggacaaag
     3301 gcccggctgg ctggtgaaca gggaaaccgc tcggtcacgg ccttctgttc gatgacggcc
     3361 atcgggaggt aatcgtcgag gggaatgaat cccatatatt caagccggat gctcaaaaag
     3421 cgttccgcgg cgacatcaag cttgcggaat gtctgcatcg cttcacgttg acttttcacc
     3481 atattgacca gcaccttgaa tcggcgctca cgatgttgcc ggcataacac cttcatgagc
     3541 gcataggcat ccgtcaatga cgtcggctcc ggcgagatga ccacgataat ctcttgagcc
     3601 gctgaggcga agtaggtgac attgggtgag acaccggctc cggtatcgat caacagcacg
     3661 tccacggcgt tcgcgacatg ttccaactca gtctgtaaga tgagttgctg tgagtcggtc
     3721 aaggaggtga ggtgaggcag gccggagcct gccggcaaca cctggattcc agcgggacct
     3781 tggaggagga tgtccgacag gtggcgtgat ccgcacagca catcttccaa cgtatgccga
     3841 ggcaccaatc ccaagagcac atcgatattg ccaagtccca agtccgcgtc cagcaccagc
     3901 acgcgtttcc cgactcgcgc caactccacg gcaaggttgg caacgatatt cgttttcccc
     3961 accccacctt ttccgctggt cacggtcagg acgcgggtgc atgggttccc gtgatccatt
     4021 gtcggcgttg ctgtctcctg ttcgctgaac atcttcatgc ggagtctccc tattacgcgt
     4081 gttgtggcgt cgttccgccc atggacacag ccgagatgcg tccgatggat gacgagaggc
     4141 ctgatgatgt gaaccgggct gagcccctgt cgagtaacag atctgccaac cgtgccgcat
     4201 ctgccagctc gacgtcttcc ggtacccgct gaccattgct ccaatacgac aggggcaacg
     4261 tggtctgata cgccaattca aagagattgc cgtattcctc cgtctcatcc agtttggtaa
     4321 aaagcagccg agacggagtg caggcgccgt accgtgccac ggtgcgacgg agatcctgca
     4381 tccgcgtggt ggcggacaag accagatgcg tgtcgatcga ttcgcacgaa tctttcagtt
     4441 gaccaaggtt cattagttgc gccgactcat gggagccgaa ccctggcgta tcgatcatga
     4501 tcaggcttgc ctggcgatgg cggcgtatgc cttcgaccac ctcagccgtg gtctgagccg
     4561 tttcgagagg taatcccagg accttcgcat acatgcggag gtgttcgacg gcggccaggc
     4621 gacaggtatc gagggtaatc agggcaaccg atctgccctc ttgaaggcga tagtgggccg
     4681 cgagcttcgc gatagtcgta gtcttgcctg ccccgctcgg tccgacaaac agggcgacct
     4741 tgtaatcatt cgccccatcc agcaatggcc cgctgactcg aatctgctcc gtgcagagtc
     4801 ggtacagcac gtcctgaatt tcgcgatcag accggagagc ctcctgtgag agatcgcggc
     4861 gaagcgtgag gcacagactt tcggccgttg atgggcgcag tccctgggca accagaagac
     4921 ggcaggctcg tgccaagggt gcgggaagac cggatcctgc cgtctgcact tcatcgggta
     4981 acgaggacgt gaggagctgc tgcaggtcaa gaagttcgtt cttgagatgg cgaaagcgcc
     5041 cctgtggaac cgggcgcgac tccggcttcg gggtcggcat cggatttctg acgttcgtgt
     5101 ccccggactc cgatggcgta tgctttttat agtgaagcaa tgcgctcaat gtgtcctgga
     5161 acgcaggagc ctgttcccgt tgactctcgg acgcatggac ctcatcctgt gcgatcgcag
     5221 aagccgcctg tcgaacagac ggagcttggt cggcactcgc atcaatcgca gcggtcacct
     5281 ctaagagcgg ccgcccgaac agcgagaacg gcaggctccc gcgccggacc cgcttcgtgg
     5341 aaaggatgag cgcatccggc cccagctctt ccttgatggc gcggatggca tcatgcatcg
     5401 tgtcagcatg aaacactttc atcttcatcg ttcacctcac ggtagaagcg cgtctccgtc
     5461 cagcctgatg gtatccaacg actggagccg gaccatcgaa tcgagttcat tgaatcccac
     5521 caccggcacg gcatggagga tccggtcggt caatcgacga atatgccgcc tgagtgccgg
     5581 tgagcagagg aggacaggct gatgaccacg ggcagcgacc cgttccaccg cctgtttcat
     5641 tgccgccaac aatgtgtgag agagaccggg atccaggttc agcatccctc cctgagggac
     5701 ggacgcagcc tgctcgctca aggaccgatc aagccgtggg tcgagcgtga tcacgggcaa
     5761 cgtgccatcc ggagcttgat actgacgcgt aatcgtccgt cccaaggcct gccgtgccat
     5821 ttcagtcagc aactcaggat ctttagtcgt actcgcgtga tcggccagcg cttcgatgat
     5881 cgaccgaaga tcgcggatcg gaatgccttc gtgcagcaag ttacctagga cacggaccac
     5941 tgctccaagc ggcagtagtg tgggaatcag ttcttcaacc agtttgggat gggccttcgc
     6001 caattcgtcg agcagcgatt gcacctcctg ccggcccaat aattcatgcc catggcgttt
     6061 aatgatttca gacaaatgtg tggcgatggc cgaactggca tccacgaccg tataaccggc
     6121 cagttgcgct tgttcgcgcg tggactcagg cacccacagc gccggcaatc caaacgccgg
     6181 ttccttcgtg gcgacaccat gaatgaggcc gcgttgggcg gttcccggat cgatagccag
     6241 cacgtgcgat ggcagcacat cggccttcgt gagctccacg cctttgagca tcaccgcata
     6301 ttcgttcgga cgcagttgga ggttgtcccg tatatgaatg ggtggcagga gaaatcccat
     6361 cgtatccgcg aactgtttcc tgagcccctt aatacgatcg agtaatgcgg ttccatgttt
     6421 gccttccacc agaccaatga gtccgtaccc gacctgtacc tccatcaaat caagcggagc
     6481 cagctgcggg gtcgtgcttt ccgattgcgg agccacggaa acgggcgccg gttcggatgg
     6541 cgccttcgcc tcctgcgaca atcgataggc taaccaggcc gcggcacccc ccaacccgag
     6601 aaacgccaga tggggcaatc cgggaacgag tccgagagcc agcaggattc ccgcagccgt
     6661 accgacggct ttgggtgaga gcaacatctg ccccgtgatc tgatgtccga gatcggcatc
     6721 cgttgcagc
//
LOCUS       DATIPF010000361         5855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_70225, whole genome shotgun sequence.
ACCESSION   DATIPF010000361 DATIPF010000000
VERSION     DATIPF010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70225"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            353..898
                     /gene="msrA"
                     /locus_tag="VLL06_09000"
     CDS             353..898
                     /gene="msrA"
                     /locus_tag="VLL06_09000"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HSS31140.1"
                     /translation="MEQTGFSIATVAGGCFWCLEAVYDQVKGVVAVESGYIGGQIDHP
                     TYEAVCGGRTGHAEAVRITFDPAVVSYRELLEVFFVIHDPTTLNRQGHDVGTQYRSAI
                     FYHAPEQKRIAEEVIAGVTKEKLYANPIVTEITPAGTWYEAEPYHQEYFVRNPFQGYC
                     TAIVGPKVAKFRKQFAEKLKT"
     gene            complement(949..1638)
                     /gene="mtnC"
                     /locus_tag="VLL06_09005"
     CDS             complement(949..1638)
                     /gene="mtnC"
                     /locus_tag="VLL06_09005"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR01691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HSS31141.1"
                     /translation="MVRYVLMDIEGTLISVAFVREILFPFAKHRLAPFLQERRHDPSV
                     LQWTVVCQDTIEHETGTRPTYEELPVLLTIWIDQDRKLAGLKGLQGMIWDEGYRQGAF
                     EPELYGDVLPTLTIWRARGIRLGIYSSGSEQAQRLLFAHTTIGDVTSLFEHYFDTSVG
                     EKKASSSYRTISEQIGLLPSHILFLSDAEAELDAAALTGFRTAHIVRPGTNASTRHPI
                     YADLTRLLLEG"
     gene            complement(1646..2173)
                     /locus_tag="VLL06_09010"
     CDS             complement(1646..2173)
                     /locus_tag="VLL06_09010"
                     /inference="COORDINATES: protein motif:HMM:NF015065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31142.1"
                     /translation="MATLRIPDKNVTLGDPSAVQQFLDRRGIVHERWSADRNLPSGAS
                     NEQVLAAYDYLLNPFMKKCGYQTMDVIRVDGHTPNLEGIREKFIREHTHSEDEVRVFV
                     EGQGFFWFHKEAQDEEVFALLCEQGDLISVPANTKHWFDLGKSPKVRAIRLFTDQVGW
                     VPHYTNSGIDQRYRW"
     gene            complement(2148..2801)
                     /locus_tag="VLL06_09015"
     CDS             complement(2148..2801)
                     /locus_tag="VLL06_09015"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: protein motif:HMM:NF006672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HSS31143.1"
                     /translation="MTHYPDFKIVAEQLVQTAQWASRMGWAPATSSNYSVRLPDDAAP
                     AYCAITSSGVDKGTIEVEHILVVDQYGKPIDAAGLKPSAETLLHLLLYRTMGAGAVLH
                     THSLSAALLSQLACNEGQVLLSGWELLKGLSGTDTHDVEISLPVFPNSQDMQALSTRV
                     EQALSNQRSCYGFLLAGHGLYAWGKDISEAKRHLEVFEYLLQCQREVTQHGHSANTR"
     gene            complement(2960..3832)
                     /gene="ttcA"
                     /locus_tag="VLL06_09020"
     CDS             complement(2960..3832)
                     /gene="ttcA"
                     /locus_tag="VLL06_09020"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HSS31144.1"
                     /translation="MSHSASSSRISLKLDEATEQLQTKLLRLVGEAIAEYGLIENGDK
                     IMVCLSGGKDSYALLDLLLVLQQRAPIHFDLVAVNLDQKQPGFPAHVLPDYLSDMGVP
                     FHIEERDTYSIVKRLIPEGQTTCSLCSRLRRGILYRVATELGATKIALGHHRDDIIET
                     LFLNLLYTGKLKTMPPKLRSKDGRHLVIRPLSAVRERDLERYAELRAFPIIPCDLCGS
                     QEDLKRKQVKAFLREWEQRSPGCTNSIFAALSNVAPPLLLDPRLFDFVGLRASNQGEG
                     QEDGWLDQDPTPLS"
     gene            3886..4383
                     /locus_tag="VLL06_09025"
     CDS             3886..4383
                     /locus_tag="VLL06_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31145.1"
                     /translation="MMRANDHGSGGNLQSVLFVCTGNIFRSVTAEFALKACLGPDTSH
                     VISSAGIDVKPQSMHTWVQARLREKGADLTIHISRQLTRELVEGADLVIAMGRNHQRF
                     VQEQFGRGVPLFNQICLGHDQPILDLHEVMPDWETDHERAAAYVYSVIDSIWATAPAL
                     LSRIR"
     gene            4561..5622
                     /locus_tag="VLL06_09030"
     CDS             4561..5622
                     /locus_tag="VLL06_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31146.1"
                     /translation="MQRLQTHEMTASDSHDQEPDWVSWPNEKLLDLPMNQLGITLEGA
                     FLSGQIQQLYAELEVRQLIFRPHFWLSNEWFTPDGVPGIAVPFYLAHPRLEKLEMDQM
                     LEVEGGTPEWCMRILRHEAGHAIENAYRLRRLRHRQHVFGRSSDPYPKYYSPRPYSRS
                     FVRHLDVWYAQSHPDEDFAETFAVWLTPDSTWADRYKGWPVLKKLRYVDGLMAGLAAV
                     PPKVVTREEIDPLSELKKTLREHYARKRTHYGTERPSLYDPDLKRLFSDAPAHADKPS
                     AATFLNRFRKEVRRKVAAWTGEYQYTIDQVLEDMIQRCRQLHLRLPLAEEQAKLDFTI
                     LLTVHTMNYLRSGRHKVAL"
     gene            5619..>5855
                     /locus_tag="VLL06_09035"
     CDS             5619..>5855
                     /locus_tag="VLL06_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HSS31147.1"
                     /translation="MKRLRILVLMHEDLVPPDPLNGQDVTSAEWKTEYDVVTTLRKLG
                     HEVKPLGVKSDLGVLRSAIEDWKPHIAFNLLEEFD"
BASE COUNT         1345 a         1543 c         1630 g         1337 t
ORIGIN      
        1 ctggccttga ctggccttta cggatcgacg aagctcaagt gcggtgggtc aaagccggga
       61 catttgggat ccagttcacg aagattcgcc ctgaagaagc ggaaaaactc tatcaggtta
      121 ttgccagtct caacgaagaa cttgacgcgt agcccttctc atttcccttt ccttcctcta
      181 attcagacta tttgatgacc accacgcacc gtgaagtgtg aggagttctg gactcgctga
      241 cggaactgtt cgcagcccca ggtcgcctta tggtataagg cagccgtagg ctctgacaga
      301 cgtgaagttt tctaggactc gttatcaatc caggtaatca gcgagctgca cgatggaaca
      361 aacgggattc agtatcgcga ctgtagccgg cggttgcttt tggtgcctcg aagcggtcta
      421 tgaccaggtg aagggtgtgg tagccgtcga gtccggctac atcggtggtc agatagatca
      481 tccgacgtat gaggctgtgt gtggcggcag gactggtcac gcagaggcag tccgtattac
      541 attcgatccc gctgttgtca gctatcgcga gttgcttgaa gtgttctttg tcatccacga
      601 tccgacgacg ctcaatcggc aaggtcacga tgtggggact caataccggt cggcaatttt
      661 ctaccatgct cctgagcaaa aacggattgc cgaggaggtg atcgcaggcg tgacgaagga
      721 gaaactctat gcgaacccga tcgtgacgga aatcactccg gccggtacct ggtacgaagc
      781 cgagccctat caccaagaat acttcgtgcg gaatcccttc cagggctact gcacagctat
      841 agttgggccg aaagtggcca agttccgcaa gcagtttgcg gagaaattga agacatagcc
      901 tcgccctgcg ttgtggatgc gttccgaaca cgtcgtcaat agggaagtct atccttcgag
      961 gagaaggcgt gtcagatcag catatatagg atgccgagta ctcgcattgg ttccaggacg
     1021 cacgatgtgg gcggtcctaa atcctgtcaa cgcggcagca tccaattctg cttccgcatc
     1081 cgacagaaag agaatgtggc tgggcagcag gccgatttgt tcactgatcg tgcggtagct
     1141 ggacgaggcc tttttctcac cgacgctagt gtcgaaatag tgctcgaaca acgatgtcac
     1201 atcaccgatg gtggtatgtg caaagagcag ccgttgtgcc tgttctgaac cagacgaata
     1261 aattcccaag cggatgccgc gcgcccgcca tatagttaac gtcggcaaga catcgccata
     1321 cagctcaggc tcaaatgccc cctgccgata gccctcatcc cagatcattc cttgcagccc
     1381 tttaaggcca gccagttttc ggtcctgatc gatccatata gtcagcagga ccggtagttc
     1441 ctcataagta gggcgcgtac ctgtttcatg ctcaatggta tcttggcaga ctacggtcca
     1501 ttggagcacg cttgggtcgt gccgccgctc ctggaggaag ggtgcgagac ggtgttttgc
     1561 gaatgggaag aggatctcgc ggacaaaggc caccgaaatg agcgtgcctt cgatgtccat
     1621 gaggacgtac cggaccatgc ggcggctacc agcgatatcg ctgatcgatt ccggaattcg
     1681 tatagtgagg gacccagcct acttgatcgg tgaagagacg aatcgcccgt accttcggag
     1741 acttacctaa gtcgaaccag tgctttgtgt tggccgggac agaaatgagg tcgccttgct
     1801 cgcacaagag ggcgaagacc tcctcgtcct gggcttcttt gtgaaaccaa aagaagcctt
     1861 ggccttcaac aaagacacgc acttcatcct cgctatgagt atgctcccgg atgaattttt
     1921 cacgaattcc ttccagattc ggagtgtggc catcgacacg aatgacatcc atcgtctggt
     1981 acccgcattt tttcataaag ggattgagca ggtagtcgta ggctgcgaga acctgttcgt
     2041 tcgaagcgcc ggaaggcagg tttctgtccg cagaccatcg ttcatgaaca atgcctcgcc
     2101 tgtcgagaaa ttgctggacg gctgatggat cacccagcgt tacgttctta tcgggtattc
     2161 gcagagtggc catgttgtgt cacctctcgt tggcattgga gaagatattc gaagacctca
     2221 agatggcgtt tcgcctcgga tatgtctttg ccccacgcat acagaccatg tcctgccagc
     2281 aaaaatccat agcaggaccg ttgattcgaa agggcctgtt ccacgcgcgt cgacaatgcc
     2341 tgcatatcct gtgagtttgg gaagaccggc aaggagatct ctacatcgtg ggtgtctgtt
     2401 ccgctcagtc ctttcagcaa ttcccaccca gagagcagga cttgtccttc attgcaagcc
     2461 agctgtgaga gtaacgccgc tgaaagcgag tgcgtgtgaa gcacggctcc ggctcccatt
     2521 gtgcgataga gaagcagatg gagaagggtt tcggctgaag gcttcagtcc cgcagcatct
     2581 atcggcttgc catattggtc gacgacgagg atgtgctcaa cttcaatcgt gcctttgtct
     2641 acccctgaac tcgtaatcgc gcaataggca ggagcggcat cgtccggtaa ccgcacactg
     2701 tagtttgagc ttgtcgccgg ggcccagccc atacgagaag cccactgggc cgtctggacc
     2761 agttgctcag caacaatttt gaaatctgga taatgtgtca cggactacaa tctgccgcct
     2821 aaagaagaag gctttgtata gcgaaatcct gacttcgacg caatggcgga ttcgccagga
     2881 ggtgcataat tgtggagccc gtatcagagc gttcgtcaga cgtcgaaaaa cccattccca
     2941 ttccagaaga ttgtgagcct catgaaagtg gagtagggtc ttgatccaac cagccgtctt
     3001 cctgcccctc cccctggtta gaggcgcgca gacccacgaa gtcgaatagc cgtgggtcca
     3061 ggagaagcgg cggtgccaca ttcgacagag cggcgaagat actgttggta cagccaggcg
     3121 atcgttgttc ccactcacgc aaaaaggcct tcacctgttt tctcttgagg tcctcttgtg
     3181 agccacagag atcgcaggga ataatgggaa acgcgcgaag ctccgcatat cgctcgagat
     3241 ctctctctcg tacggcggac agcgggcgaa tgaccagatg ccggccatct ttcgagcgta
     3301 gtttcggggg catcgtcttg agcttgccgg tatagagtaa gttcaggaac agggtttcaa
     3361 taatgtcatc gcggtggtga cccaatgcga tcttggtcgc gcccaattcc gtggcaacgc
     3421 gatagagaat gccgcggcgg agccgggagc agagcgaaca ggtggtttgc ccttcaggga
     3481 tgagccgttt gacgattgag taggtgtctc gttcctcgat atggaatggc actcccatat
     3541 ctgaaagata gtcgggaagg acgtgggcag gaaacccggg ctgcttttga tccagattca
     3601 ccgcgacaag gtcaaagtga atcggagcac gctgttgtaa gaccagcaat aggtcgagca
     3661 gcgcatagct gtccttcccg cctgataggc agaccatgat cttgtcgcca ttctcaatga
     3721 ggccatattc ggcaatggcc tctccgacaa ggcgaagcaa cttggtctgc aactgttccg
     3781 tcgcctcatc cagtttgaga gatatgcgtg atgagcttgc tgaatgtgac atagacgggc
     3841 attctacgcg tgtagctatg cgactgtcga ccgtaagctg gaggtatgat gagggcgaat
     3901 gatcacggtt ccggtgggaa tctgcaatcc gttctatttg tatgcacggg gaatattttt
     3961 cgaagtgtga cggcagagtt tgcgctcaag gcctgcctag gccctgatac atcccacgtt
     4021 atcagctcgg ctgggatcga cgtgaagcct cagtccatgc atacctgggt tcaggcccga
     4081 ttgcgtgaga aaggcgctga tctcaccata catatctcac gacaattgac cagggaactg
     4141 gtcgaaggtg ccgacctcgt gattgcaatg gggcgaaacc accagcgctt tgttcaagaa
     4201 caatttgggc gcggcgttcc tctgtttaat cagatctgtc tcggtcacga tcagccgatt
     4261 cttgatctgc atgaagtgat gccggattgg gagaccgacc atgagcgggc tgccgcctat
     4321 gtgtactcgg tgatcgatag catctgggcc acggcaccag cccttctttc tcgcatccgt
     4381 taacccattt tcatgacaca gattgtcagc ggcggttcat tcttggactc gattggacac
     4441 tttttctcca cttattagca gtggtcagtg gaatagcctc ctcaatcgga gcaatattga
     4501 agtacgtgct atattttcca cagcacctct tccagattga tttcgaagaa tacggtatcg
     4561 atgcaacgac tacaaacaca cgaaatgaca gcgtctgaca gccatgatca ggaaccggac
     4621 tgggtctcgt ggccgaacga gaagctcctg gaccttccta tgaaccaact cggcatcacg
     4681 ctcgaagggg cattcttgtc aggacagatt cagcaactct atgccgaact cgaagtcaga
     4741 cagctcatat tccgtcctca cttttggttg tccaacgaat ggtttactcc ggatggtgtg
     4801 ccggggattg ccgtgccgtt ttacctggcc catccacgac tcgagaagct tgaaatggac
     4861 cagatgcttg aggtcgaggg cgggacaccg gaatggtgta tgcgaatttt gaggcatgag
     4921 gccggccacg ccatagaaaa tgcgtatcgc ctccgccgtc tccgccatcg gcagcacgtc
     4981 tttggacgct catccgatcc ctatcccaag tattacagcc cccggcccta tagtcggagt
     5041 ttcgtgcggc atctcgatgt gtggtatgcg caaagccatc cggatgaaga ctttgcggag
     5101 acctttgcgg tctggctcac gccagattcg acctgggccg accgctacaa gggctggccg
     5161 gtgctcaaaa agctgcgata tgtcgatggg ctgatggcgg ggctggctgc ggtgccgccc
     5221 aaggtggtga cgcgtgagga aatcgatccg ctgtccgaac tgaaaaaaac tctgcgcgag
     5281 cattatgcac gcaagcgtac acactatgga accgaacgac cgtcattgta tgatccggat
     5341 ttgaagcgcc tattttccga cgcccctgcc cacgccgata agccaagcgc tgccaccttc
     5401 ttgaaccgct ttcgaaaaga ggtacgtcga aaagttgccg cctggaccgg cgaatatcaa
     5461 tatacgatcg atcaagtgct cgaagacatg attcagcgct gccgccaact ccatctacga
     5521 ctgcccctcg cagaggagca agcgaagctg gatttcacga tcttgttgac tgttcacacg
     5581 atgaactact tgcgcagcgg acggcataag gtggcgctat gaaacggctc cgcatcctcg
     5641 tcttgatgca tgaagatctg gtgccgccag atcccctcaa cgggcaggac gtgaccagtg
     5701 cggagtggaa gacggaatat gatgtggtca cgacgctccg caagctcggg catgaagtca
     5761 agccgctggg ggtcaagagt gatcttgggg tgcttcgatc ggccatagaa gactggaagc
     5821 cgcacattgc cttcaatctg ctcgaggagt tcgac
//
LOCUS       DATIPF010000362         2895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1664988, whole genome shotgun sequence.
ACCESSION   DATIPF010000362 DATIPF010000000
VERSION     DATIPF010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664988"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..528)
                     /locus_tag="VLL06_09040"
     CDS             complement(247..528)
                     /locus_tag="VLL06_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31148.1"
                     /translation="MSILTVEKFMKSLPAPRFRSANWQQYENKELWVADAVAKTGQRE
                     WLWIDDAMPPKERLEYLALDPRRCVKVNPKGANELVTLQGTLLQLLSRS"
     gene            complement(971..1357)
                     /locus_tag="VLL06_09045"
     CDS             complement(971..1357)
                     /locus_tag="VLL06_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31149.1"
                     /translation="MMNRIGHIISVPTVCLAIMSLSLIGMTGYTFAGDQILSSDLVSR
                     VSSQGTADEHRGAALVYRERARQLEAEAAQYAKEAAGIKEDTKGFRRGALKMAAGSLQ
                     SQADEMKQLSINHQKQADSMTAHQKE"
     gene            complement(1961..2182)
                     /locus_tag="VLL06_09050"
     CDS             complement(1961..2182)
                     /locus_tag="VLL06_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31150.1"
                     /translation="MANWLNDVRTYVMIVEPDVNIGINLTDRLAAHGYRAVLVRSVEE
                     VIEELSDIRPQLVFVGGGPFQARSTDRNI"
     gene            complement(2194..2487)
                     /locus_tag="VLL06_09055"
     CDS             complement(2194..2487)
                     /locus_tag="VLL06_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31151.1"
                     /translation="MVQAQVSNVDSVICHEVARLVSCPLDELAERLPVYSWAQVFAAV
                     DRLSRKGTLRLNRTSRFGYALSVCSDPSLSSLRETGEARHCADRTTDSSRAGQ"
BASE COUNT          644 a          759 c          765 g          727 t
ORIGIN      
        1 ggtctgtagc cttttgctct gtatggagaa taggatcagg cagtgactag tgcacccgtg
       61 ggtataccac atcgaaatct ggaccagtta gccagttgtc aatcgccaga ataaccgttt
      121 ccttccgcat ctctggcagc tcgccaccaa gctggttgta tggccccgct cgcttctgcg
      181 cagcctcatc tgatacgcgt caatcgcgtg ggtgtttgag tgaaggaggg gggagagaga
      241 atggtcttag gaacggctaa gcagttggag cagcgtccct tggagggtta cgagttcgtt
      301 tgctcctttt ggattaacct tcacgcatcg acgcggatca agggcaaggt actcaagcct
      361 ttccttaggt ggcatcgcgt cgtcaatcca gagccactca cgctggcctg tctttgcgac
      421 tgcatcagca acccaaagtt ccttgttttc gtattgttgc cagtttgctg aacggaagcg
      481 aggggccgga agggatttca tgaacttctc tacggtgaga atagacaaca gcgtgtcatt
      541 cgacgtgtgt gaatcatagc tgcgagtttc catcatgtgt ttgagatggc ccaggaagag
      601 ggaattctgc aattccgttt gacgtatcaa tggatagttt ctggatgaca gaactgaaag
      661 aaacggaggg ccagcttccc gctacaacag agctacacat tcctggaagt tggttctcct
      721 cggcggtttt ccacgaggta gggaataaaa tccgtccaat cctggtgtcg atggaatctg
      781 ggatcgtgac agtctaagaa cgtgactctc aaaaagcaca ggtccagctg acctgctcct
      841 ttgtgatggc caagcctcat atccaacccg gacaggttta cagggagcag gtcagcttcc
      901 atactatctc atgtccccag gcaccaatcc gcgagaatca gacgagtaca agcgcgtcgt
      961 catgttacta ctactctttt tgatgcgctg tcattgaatc ggcttgcttc tgatgattga
     1021 tggacagctg cttcatctca tcagcctggc tctgtagtga ccctgcagcc atctttagcg
     1081 caccacgacg gaatcctttt gtatcctctt taatgcctgc cgcttccttg gcatactgtg
     1141 ctgcctcggc ttcaagttgg cgtgcgcgtt ccctatatac tagggctgcg ccacgatgtt
     1201 catcggcagt accctgagat gaaacgcggc ttacaagatc gctggatagg atctgatccc
     1261 ctgcgaacgt atagcctgtc attccgatca gtgagagcga catgatcgct agacaaacag
     1321 tgggcacgct tattatgtgg cctatcctat tcatcatcca cctccttgaa tcttgacggg
     1381 tgaatcacgc cgagactctt tggtgtctac ttccttctca ttttccgggt accaacatgc
     1441 atttatgacc catggcatca atcaatgtga tggccattgt gacatacctc ctttcgtaag
     1501 cattcatagg ctcctgggtc gaaagatggg ctaatgtcgc atatcgcaat ctgttcacat
     1561 agttcgccca ctcccacagt tcttccacgg gccaagcact cgttggacag gaaaaaggcc
     1621 cggcaccgtt cagtgtcgag cctcttctga gagcaagccg ggtctccgta tcacctgcac
     1681 gacattgtgc tttccccgtc gaaacgtctc gctgtgcctg cacgacgctg tgcctgcacc
     1741 gggtctccat ggcgatgttc tgagtcggtt gtgctgatag ttccacaacc atgtgtctgc
     1801 tctgacttat gaccgcttga acgatccacc tcagaccggc ttgactagaa cgtggtggac
     1861 ctttcggcga aacataccct gtgtcatgtc atcgttggtc tgatccgcga ttgtgatcat
     1921 cggtacggcc ggacatccgg tccggatcaa acggagtatt tcagatattt cgatctgtgc
     1981 tccgggcttg gaatgggccg cctccaacaa acaccaactg agggcggatg tcgctgagtt
     2041 cctcgatgac ttcctcgaca gatcgaacga gcaccgcccg gtagccgtga gccgcaagcc
     2101 tatcggtgag gttgatccca atgttcacat ccggctccac aatcatcacg tacgttcgaa
     2161 catcgttcag ccagttcgcc atcgcaagat ctcctactgt cccgcacggc ttgaatcagt
     2221 cgtcctgtcc gcacaatgcc tggcttctcc ggtctcacga agactggaga gggaaggatc
     2281 tgaacacacg gatagggcat agccaaaacg acttgtgcgg ttgagccgta gcgtgccttt
     2341 ccggctgagc cgatccaccg ccgcgaacac ttgtgcccaa gaatagaccg gtaatcgctc
     2401 agccagctca tcgagagggc aggatacgag cctggctact tcgtggcaaa tgacggaatc
     2461 aacgttggag acctgcgcct gtaccatcac atcctcctcg ttgagaatga actgggttct
     2521 ggattgattg gtcaggtggc ctcgagaagg tgttgaggag agtaccattg tcaatgtgag
     2581 atctgttcat tgctcgtgta tgagaagtta tagccgagtc ctggtctaag ccccatcagc
     2641 cgagggagaa ttttcttgtc ggattgagac cagcagcact gtcatggttt gtacgacaga
     2701 ggcgaatgac gcgctctgtt gtctatcgaa tatgcggttt ctcggcggac cgtcacatga
     2761 gtaatggtgg tgatcaagct ctatattctg agagaaagag tgtgggtggg tggatctgca
     2821 agaagaacgg ggcaaggcaa cgaaggccaa cgcgactatt cagaaattca tttactcagg
     2881 tcgcacgggg ggttg
//
LOCUS       DATIPF010000363         6384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1484788, whole genome shotgun sequence.
ACCESSION   DATIPF010000363 DATIPF010000000
VERSION     DATIPF010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484788"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VLL06_09060"
     CDS             <1..681
                     /locus_tag="VLL06_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HSS31152.1"
                     /translation="EETEREAQKGLKRKLSEQFSRARAEVQATVDAVKGEQKLIKAKA
                     AKQRLFELEVETRAELAPAGASIPLAQLGVGDQVEISGLGITGILLEVPQGKKRVRVK
                     VGEGEVLATVANLVGLAPGQGAASKPAPSSNARRFSSGGGLGLDEQTAVDVRGKAADE
                     ALDDVIAALDRATLAGVPFLRIIHGHGTGKLKVSLRDYLKYSPYVAEFRAGDRAEGGD
                     GVTVARLR"
     gene            complement(659..1342)
                     /locus_tag="VLL06_09065"
     CDS             complement(659..1342)
                     /locus_tag="VLL06_09065"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HSS31153.1"
                     /translation="MLRWSPSTQKHACLAMLLLAASLIEGQVFGAPREQLIPILGVTT
                     GQNQIGTVSYVRVSFDERQDQTGLMLHFRTTPGRFSRMAQTSIEQAIRRSARSLGVSI
                     DSWTVELSVPYAGMTIYGDSVSAMVSLSVAAMAQGKTVSTGHVLTGTVTPDGEIGPVG
                     SIPLKVQAARAAKLRRVVVSKHTPAGEHAGNLPVSIQVSPVQSVPEALDALTDSPPMK
                     STISEVEQR"
     gene            1877..2860
                     /locus_tag="VLL06_09070"
     CDS             1877..2860
                     /locus_tag="VLL06_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31154.1"
                     /translation="MNEGLIMIIFRAIMFFSIALFVVGVFKPEVFRFRQKQPNRMTIL
                     GVAIGLFIVGVTGSGTNQFAGKDNVEAVNRGKMIGQAVYEVDNSTRRCEPGTKPGNAG
                     SSKDEQTSAGIRYMVKTPANYNSNIAHPLLMVYAPGGRNRYESEDYMYLTQVGTTAGF
                     VVAYADHRTMTSKAIEELAEIPGLIEKKWCIDKQRIFLTGHSDGGTVAMGIAFLAGTK
                     HIPAAIAPSAVGIRGDDLKEQSCPGPLPVMVMHSSRDTHFPGYGKETIRWWAACNGCD
                     TAGPVRDGDGCVTYASCKNNVTTRYCEGTGTHPEWPGNNKAIIDFLRAGHK"
     gene            complement(2776..3555)
                     /locus_tag="VLL06_09075"
     CDS             complement(2776..3555)
                     /locus_tag="VLL06_09075"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSS31155.1"
                     /translation="MESLMEFLAGRRASNGEALLPFSAPTYEQIRRFFGDLVRAVYRL
                     EMIGADRIPVSGPIVVAPNHDSVLDGIVLGTAISRELRFVAKAELWRSRLLAWALNGL
                     GAIRIERGRSDHRALAQVRHALDVGQAVAIFPQGAVRGDRVWHRGAARVALVTGAPLV
                     PVRLIGTAQAFSRGRIAFPRLRVILGEPIGVARAPEDPVAAIPSCRVPKKPSRKLDCA
                     IADSIHYASIQRPGYLCPALRKSMIALLFPGHSGCVPVPSQ"
     gene            complement(3903..4394)
                     /locus_tag="VLL06_09080"
     CDS             complement(3903..4394)
                     /locus_tag="VLL06_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31156.1"
                     /translation="MDQDPCVRQAGESLVHFSAYQPQYELKDQYCTEIPQEGHTFLVV
                     DLVDPMLRNEPVGMRVVKGTGKDEAESQIVADVRPSYHPDGVLRGEVRLDEGLYTVTI
                     ASEKQNLLKRPQYLLRVHMIDYQKAVLSIAGPLIGVLLCAWLVLKLIRSKWLQRWWAA
                     PSS"
     gene            complement(4744..4938)
                     /locus_tag="VLL06_09085"
     CDS             complement(4744..4938)
                     /locus_tag="VLL06_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31157.1"
                     /translation="MRCARCNGLMVREKFEDLGGLGSGDHESAGWRCINCGAIVDHVI
                     IAHRRLASQAAASNPALTTV"
     gene            complement(4999..5193)
                     /locus_tag="VLL06_09090"
     CDS             complement(4999..5193)
                     /locus_tag="VLL06_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31158.1"
                     /translation="MKKAGKNNAVQCWYPKLIHTQKGGIPMTTTAAQNNDHTMKHIMF
                     IILGAVTVMCLALGTGYLQP"
     gene            complement(5171..5437)
                     /locus_tag="VLL06_09095"
     CDS             complement(5171..5437)
                     /locus_tag="VLL06_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSS31159.1"
                     /translation="MTIRIEKETHKLRTMIRLSGRLQSKHLDELKTQLEGTQSRIALD
                     LNGVTLVDVEIVRFLNACEKGGVEILHCWPYIQEWMSREKGREE"
     gene            complement(5692..>6384)
                     /locus_tag="VLL06_09100"
     CDS             complement(5692..>6384)
                     /locus_tag="VLL06_09100"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSS31160.1"
                     /translation="ADGGTILLDEIGDLPAETQIALLRVLQEKEIERVGGSQPIPIDV
                     RVLAATNRDLKAAMAAGTFRQDLFYRLNVFPIQIPSLRERTDDIPLLVTYMIERYAKK
                     AGKKIRNIQKETMKLFQAYNWPGNIRELQNVIERAVVLCDSETFSVDETWLKPEGHRL
                     SGSVVPLGATLAEHEKEIIEAALADCAGQVSGATGAAAKLGLPRQTLESKITTLGIDK
                     HRFKTRQTDYAR"
BASE COUNT         1376 a         1739 c         1852 g         1417 t
ORIGIN      
        1 gaagagactg aacgtgaagc acagaagggc ctcaaacgaa aactcagcga acagttcagt
       61 cgcgcgcgag ctgaagtaca agccacggtc gatgccgtca agggcgagca aaagctaatc
      121 aaagccaaag ccgcgaagca gcgattgttt gagctagaag tggagacgag agccgaattg
      181 gccccggctg gtgcgtcgat tccacttgca cagttggggg tgggggatca ggtcgagatc
      241 agcgggctcg gcatcaccgg tatactcctc gaagtgccac aaggaaagaa acgggtaagg
      301 gtgaaggtgg gcgaaggaga agtgctcgca accgtggcga atctcgttgg ccttgcacca
      361 gggcagggag cagcgtcgaa gccagcgcca tcgtcgaacg ctcgacggtt ctcgtcaggc
      421 ggtggattgg ggcttgatga acagacggcc gtggatgttc gaggaaaggc tgcggacgag
      481 gcgttggatg acgtgattgc ggcgctggat cgtgccacct tggccggcgt gccgtttctg
      541 cgcatcattc atggccatgg cacagggaaa cttaaagtgt cgttacgaga ctatttgaaa
      601 tactcaccct acgtggcaga gttccgtgcg ggagatcggg cagaaggcgg ggatggcgtt
      661 accgttgctc gacttcgcta atggttgact tcatgggtgg tgaatcggtg agggcgtcca
      721 gggcttcggg taccgattgc accggcgata cctggatcga gaccggaagg ttccctgcat
      781 gttcgccggc aggtgtgtgc ttcgagacca ccacccggcg gagtttagcc gcccttgccg
      841 cttgcacttt tagcggaata gatccgaccg ggccgatctc accatcggga gtcaccgttc
      901 ctgtcagtac gtgaccggtc gacaccgtct tgccttgtgc catggctgcg acgctgaggc
      961 tcaccatcgc cgatacgctg tccccataga tcgtcatgcc tgcatagggg acagaaagtt
     1021 cgacggtcca ggagtcgatg gagactccca gagatcgcgc gctccgtctg atcgcctgtt
     1081 cgattgaagt ctgcgccata cgtgagaatc ggccgggtgt cgtgcggaag tgcagcatca
     1141 gacctgtctg gtcctgtcgt tcgtcgaacg agacccgtac ataggagacc gtgccgatct
     1201 ggttctggcc ggtcgtcacg cccaggatgg gaatcaattg ttcccgcggg gcgccgaaca
     1261 cctgtccttc aatgagggag gccgccagta gtaacattgc gaggcaggcg tgcttctgtg
     1321 tcgacggcga ccagcggagc atggagccct cgtgctcgag tgattctgtg aatggacgtg
     1381 tgtgctatcg gttatgcagg gagagtcttg aggggtcgca taggcagtgc gcgaggatga
     1441 gcgagagcag atacggacag acagcattcg acagaaagaa gcgcggatcg tcaattctac
     1501 cgcacgtctt cgttgcgttc tgtttgtggt ggttacaaga gagggaagag ccaaccatcc
     1561 ttcttgctcg ccgaggccgc gcgatcagcg cgatcagatt gtgctccttc tctgcgtgca
     1621 gtgaaggagc agtcgcagct ttcttaaagc caatatgtgg ggaccatcgg agacacctca
     1681 tcagggctac gggcacgtta tggaggatcg attgcgaaat aatcggaggg cagggtggat
     1741 gaagaatatc tgtcagcgga cgggcgagtg agaaggtcgc gcgcagtttc gagattcaga
     1801 cagtggagcc gcgctcttac aatgaaacgc agatcagtat aacctcctat agggtgaacg
     1861 gtgtgagaga caaccgatga acgaagggct catcatgatc atcttcaggg cgatcatgtt
     1921 tttttcgatt gccctgttcg tggttggtgt cttcaaaccg gaggtgttcc gcttccggca
     1981 gaagcaaccc aatcgtatga cgattcttgg cgtcgcgatc gggctattca tcgttggtgt
     2041 gactggctca ggcacgaatc aatttgcggg gaaggacaat gttgaagctg tgaatcgcgg
     2101 caagatgatc ggtcaagctg tgtatgaagt cgacaacagt acgcgacgtt gcgagccagg
     2161 aacaaagccc ggcaatgcag gctccagcaa ggatgaacag acatcggcag gtattcgata
     2221 catggtgaaa acgccggcga actacaattc aaacatcgcc catcctttgc tcatggtgta
     2281 cgcaccagga ggaagaaatc gatatgagtc ggaagactat atgtatctca cccaagtagg
     2341 aacgacggcg ggttttgttg tggcatatgc agatcaccgc acaatgactt ccaaagctat
     2401 tgaagagctt gccgaaatac cgggactgat tgaaaagaaa tggtgcatcg acaagcaacg
     2461 gatttttctc accggtcatt ccgatggcgg aactgttgcg atgggtattg catttctcgc
     2521 cggcaccaaa catattcccg cggcgattgc tcccagtgcg gtgggtatca ggggtgatga
     2581 cttgaaggag cagagttgcc ctggcccgct tccggttatg gtcatgcaca gcagccgtga
     2641 cacccatttc ccgggttatg gaaaagagac gatacgatgg tgggctgctt gtaatggttg
     2701 cgatactgcc ggtccggtaa gagacggaga cggctgtgtc acgtacgcgt cttgtaagaa
     2761 taatgtcaca acacgctatt gcgaagggac agggacgcat cctgagtggc cgggaaacaa
     2821 caaggcaatc attgattttc ttagggcagg gcacaaatag cccggacgtt gaattgacgc
     2881 gtagtggatt gaatcggcga tcgcgcaatc caactttctc gaaggcttct tgggcacacg
     2941 acacgagggt atcgccgcga cgggatcttc cggggcgcgc gcgactccga tcggctctcc
     3001 gaggatgacg cgcaaccgcg ggaacgcgat tctgccacgt gagaaggcct gtgccgtgcc
     3061 gatgaggcga accggcacaa gcggtgcgcc tgtcacgagt gccacgcggg cggcaccgcg
     3121 atgccacacg cggtcgccgc ggacggcgcc ctgtgggaag atcgcgaccg cctgtcctac
     3181 atcaagggcg tgtcgtacct gcgcgagcgc gcgatggtcg ctgcggccac gctcgatcct
     3241 gattgcgccg agcccgttga gcgcccaggc gagtagtcgg gaccgccaga gttccgcctt
     3301 cgcgacgaat cgcaattcac gtgagatcgc tgttcccagg acgatgccat cgagcaccga
     3361 gtcgtgattc ggcgcgacga cgataggacc gctgacggga atacgatcgg cgccgatcat
     3421 ctcaagtcta tacacggcgc gcaccagatc gccaaagaag cggcgaattt gctcataggt
     3481 cggagcggag aacggcagga gggcctcacc gttgcttgcc cgacggccgg caagaaattc
     3541 catcagtgat tccatcagcc ctcctcctca tccttgactg ctgcgctcac gcgttgcggt
     3601 agtcatgtgg ccctgttctc tacaccacag ggttaggcat catgtctgtg tggtttctct
     3661 cgtctccaag ctccccaggc agagtaaaat cccaacggcg cacttatggc gcaaatccat
     3721 gccagagtgg gcgaatctga ttccgcgaac agccaaccgg caagtacgga atagagcacc
     3781 cctccgacac agtagacgta ccaaggaatc ttgctctttc tgctcaacgc cctgtcggtt
     3841 tgcgatgtca ttcgaatgcc ctcacgcatc cagtgatgtc ttgcgaccag atgactggcc
     3901 ttttacgaac tgggggccgc ccaccatctc tgcagccact tcgatcgtat aagtttgagt
     3961 acgagccagg cacagagcag cacacctatc agtggtcctg cgatggaaag caccgctttc
     4021 tgatagtcga tcatgtgtac ccgtaagaga tactgcggcc gcttcagaag gttctgcttc
     4081 tcggacgcga tggtgaccgt atagaggccc tcatccagcc ggacttctcc cctgagcact
     4141 ccgtccgggt ggtagctcgg acgcacgtcc gccacaatct gactttccgc ctcatcctta
     4201 ccggtaccct tcacgacacg catcccgacc ggctcgttgc gtaacatagg gtccacaagg
     4261 tccacgacta agaacgtgtg gccttcctgg gggatctctg tgcagtattg atctttgagt
     4321 tcgtattgcg gctgataggc gctgaaatgc accaggctct cccctgcctg gcgcacgcac
     4381 gggtcttgat ccatctccgt gcctccatgt gctgtggcta ggtctgggtg gtacagtgct
     4441 gcaacaagca ggagcatcgt tgcgcctgct cctatcacgt gccttatcat ggccggaccc
     4501 tcctgttttg aacgttacga tgagttcctg ctcgatcaac ccagggtgac gctgctccgt
     4561 gctgccttcc tcgtcactcc aggcagccat gctccttcat tctggtcgtc ctcttctgtt
     4621 cctcaccgag gagacaggcc tctccggggt gtaaggaggg gcctgtctac ctcagcggga
     4681 gacgccgtgc tgtcacggcg ccaccaagat gcaacatata cagtacgcat gaaggacgct
     4741 tccttacacg gtggtcaatg ccggattaga tgccgctgcc tgcgacgcga gccgccgatg
     4801 tgcaatgatc acatgatcga caatcgcccc acagttgatg caacgccatc cggcagactc
     4861 atggtcaccc gacccgagcc ctccgaggtc ttcaaacttt tcgcggacca tcaatccatt
     4921 gcaccgtgcg catcgcatcg catgcctcct tcattgacga gaccctgtgt ctcccgcttc
     4981 acacctttgc ttccagggct aaggctggag gtagccagtg ccaagtgcca ggcacatgac
     5041 agtcacggcg ccaagaatga tgaacatgat gtgcttcatg gtgtgatcgt tgttttgcgc
     5101 agctgtggtg gtcattggaa ttcccccttt ttgggtgtgg atcagttttg gataccagca
     5161 ctgcaccgca ttattcttcc ctgccttttt cacggctcat ccactcctgg atgtatggcc
     5221 agcaatggag gatctcgaca cctcctttct cacacgcgtt caggaatcga acgatctcga
     5281 catccacgag cgtcacgccg ttgaggtcca acgcaattcg cgactgagtg ccttcaagct
     5341 gcgtttttaa ttcgtccaga tgcttcgatt gcagccgacc gctcaaccga atcatcgtcc
     5401 tgagtttgtg ggtttctttt tcaatacgga tggtcatatg cgactcctac aacagatgac
     5461 aatacctagt gcaatgagga gaccacatat aagtatttga tatacttaca tgcagagtga
     5521 attattttgc cgaactatca gcaattgccg aatgttcggc aaaatttgaa gcgggagctg
     5581 cagaagttct aatttcccga atggacgatg atgtggagag cgataaagtg gggcgaagtc
     5641 agaacaacgg tcgacaggtt ttacagttga cctgcaaaca acatgtgatt tttaccgtgc
     5701 atagtcagtt tgtcgagttt tgaatcggtg cttgtcaatt cccagggtcg tgatttttga
     5761 ttccagcgtc tgcctcggga gccccagttt ggccgcggcc ccggtagcgc cgctgacctg
     5821 gcccgcacaa tccgccaatg cggcttcaat gatttccttc tcatgctcgg ccagtgtggc
     5881 gcctaaggga accaccgatc ctgatagcct gtgtccttcc ggtttcaacc aggtctcgtc
     5941 gacagaaaac gtttcgctat cgcacagcac gacagcccgc tcgatgacat tctgcaactc
     6001 gcgaatattg ccgggccagt tataggcctg aaacagcttc atggtctctt tctgtatgtt
     6061 ccggattttt ttcccggcct ttttcgcata acgttcaatc atgtaggtga cgagtaaagg
     6121 aatatcgtcc gtccgttcgc gcagagaggg tatctggatg gggaagacat tcagcctgta
     6181 gaaaagatcc tggcgaaatg tgcctgctgc catggccgcc ttcaggtctc ggttcgtggc
     6241 ggccagcacc cgcacgtcta tgggaatcgg ttggctgccc ccgacccgtt caatttcctt
     6301 ttcttggagc actctcaaca gggcgatttg tgtctcggcc ggcaggtccc cgatctcgtc
     6361 aaggaggatc gtcccgccat cggc
//
LOCUS       DATIPF010000364         6207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_956149, whole genome shotgun sequence.
ACCESSION   DATIPF010000364 DATIPF010000000
VERSION     DATIPF010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956149"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VLL06_09105"
     CDS             complement(<1..186)
                     /locus_tag="VLL06_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HSS31161.1"
                     /translation="MAEFTHFNESGRARMVDVSAKDSTERIATAQATVFLQPETLEKI
                     QRGKIAKGDVLSVAQVAG"
     gene            complement(604..1245)
                     /locus_tag="VLL06_09110"
     CDS             complement(604..1245)
                     /locus_tag="VLL06_09110"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HSS31162.1"
                     /translation="MEKLVRGFLKFRGEVFGRKKDLFTRLSQKQEPRALFITCSDSRV
                     DPTLMTQAEPGELFILRNAGNMVPPYGSMQGGSTATIEYAMAILKVPHIIVCGHTDCG
                     VMKALLHPEDVQDLPAFKEWVAQAETTRRLMREHYVDLKGNERLIKTTQENVRAQLDH
                     LRTHPSVALQLRKGKVDLHGWVYSILTGDIWVYDFDSEQFTSLLDEVSPRLNT"
     gene            complement(1260..1388)
                     /locus_tag="VLL06_09115"
     CDS             complement(1260..1388)
                     /locus_tag="VLL06_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31163.1"
                     /translation="MDDLVKIKWLARLILVVYEHFRLLITTMLMTVLMVEISSYGR"
     gene            2578..2850
                     /locus_tag="VLL06_09120"
     CDS             2578..2850
                     /locus_tag="VLL06_09120"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative inorganic carbon transporter subunit
                     DabA"
                     /protein_id="HSS31164.1"
                     /translation="MKSGGAGSDLRTGLPFQMVWMHEPMRMTFVFEGLPAIVSQIVQK
                     HCPLQKLFDNRWLHLIVLDIRTDQFVRYESQGQWAPVSIRQPALST"
     gene            3008..4708
                     /locus_tag="VLL06_09125"
     CDS             3008..4708
                     /locus_tag="VLL06_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSS31165.1"
                     /translation="MFSIILVPFLPLLAALIVLVGDENSRRRRARIAAYPIGAALLGA
                     IVTLWYVASHGAITIRFYDPSSPATLAFPIGFYIDRLSAIMMVLISGVGTIIYTYSVG
                     YLYQDPHETRYLSLIGFTTSVLLCMVSSGNLMMLFIFWQLLSYLLYILAHNHAHAATL
                     EGAFRTFTLLRVGDVAFLSGIVLAHSLYGTLEFQSLFVKAAETPVTLSPLPGWELNGA
                     TAVTLLLFIGGMSKSAQFPMHVWLPRSLYAPTPVHALLHAGIINAGGFLINRLAPLFG
                     LSSTTLHVAFAVGTLTAVLGASMMLAQNDIKKTLGFSTIGQMGYMIMECGLGAFSLAV
                     FHLIAHGLFKATVFLNCGNVIHKARQEPHFPHHDHQEKEEGFSRLTWSTGFVTTLFIP
                     LLILLVTHGVLHIPLLESQGTVIFLFFIWITSSQAIMTLTRLRAVASWKVSSVMLLTL
                     LFVVFVYLFAVESFTAFLYPNPQEVASYFKAAELPYWFFDLMIIMATLTTIAGWFYLY
                     MRAHGRSVWMPSWVEGVRIRLYVLFMNRLYADEVYQKVGGVAGRLLDRFDKSVRGWSR
                     "
     gene            4708..6015
                     /locus_tag="VLL06_09130"
     CDS             4708..6015
                     /locus_tag="VLL06_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSS31166.1"
                     /translation="MDGTVATWLLVGIPLLGAAFGFAVWSRPHYLRNWSVVLSILSLV
                     AAVSMSGSLVTSPENLLLVYLLPVAACASLLGQPVHKNLRLSWITTLLFLGLGFVILT
                     SQNVIGQVCLLVLLSSLIALLYRHHTPLWPISWLGIGAYALAAGCVTISLVVAPPLSS
                     VASLLACAVLLPLMPFHQGHITALTRLPGGLPSFIVLLFPVIGLHGATSVMPTVPDAA
                     AWTVSLLALAGTFYGAVKALAQSRARLLLSYSSLSFYSMLWWFGASTNATTPRAAVFV
                     GAVGFTTSGLLLAWQVIRTRYGDDVDPQSISGLAATMPRYAVLLSLLALAAIGLPPFG
                     LFAGFMGLLLLSKISFSIAIVIIVTAWLAASWYIMGMAQQWLFGTRRYDLRYTDLLPG
                     ELASLTILVVVLLALGVVPTHLFESGTLTAATGAITELFVWNK"
     gene            6003..>6207
                     /locus_tag="VLL06_09135"
     CDS             6003..>6207
                     /locus_tag="VLL06_09135"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative inorganic carbon transporter subunit
                     DabA"
                     /protein_id="HSS31167.1"
                     /translation="MEQVENRPDIEARRMELRGIVHLAGEVVAQYWPMRTFVHHNPLH
                     SLEYLPFEETVQRGKQFLGGNGYL"
BASE COUNT         1220 a         1743 c         1699 g         1545 t
ORIGIN      
        1 gccggcaacc tgtgcaacgg acaagacatc gcccttggcg attttcccac gctgaatctt
       61 ttcaagggtt tctggctgga gaaaaaccgt agcctgagct gtcgcgatcc gttcggtcga
      121 gtccttcgcg ctgacatcga ccatacgtgc ccgccccgac tcgttgaagt gcgtaaattc
      181 tgccattttg tgagctgaat cagccggttg tagaagaaaa gtgaggtgat tatagaagcc
      241 gctggaaagg gcagtcaagg gcgacctgga tggtccctct cgctcgcaga gtgcgcacga
      301 tcagaatgtg ctccctccat gcgcgcggta agggaccaat ccaggccacc cttgagggag
      361 taagtgaagg gaaggaaaaa gatggtctcg tgctcccgga acagaggcgg gattgagcct
      421 gtataatttt ttgtggtcgc gcaacgcgcg gtctgagaag gcactcgctc gggcaaatgg
      481 cacatctcgg cgtgccagtg ggtggacggg tgagaacgtc gcgcgcagtt ggggatctaa
      541 ctaggacacc ccctcccctt ccctgagaac atgagcatct tcggtcgcga tgaaaatgcc
      601 actctaggtg ttcaagcgtg gagagacttc gtccagcagt gaggtaaatt gttcgctgtc
      661 gaagtcgtag acccaaatgt cgccggtcaa aatcgaatag acccagccat ggagatcgac
      721 tttgcccttg cgcaactgga gggcaacgga gggatgggtg cgcaggtggt ccaattgggc
      781 gcgtacgttt tcctgtgtcg ttttgattaa tcgttcattc cctttgagat cgacatagtg
      841 ctcgcgcatc aagcgtctgg tcgtttctgc ctgagcaacc cattccttga aggccggcaa
      901 gtcctgtacg tcttcagggt gcagaagggc tttcatgacg ccgcaatcgg tatggccaca
      961 gacaatgatg tgagggactt tcaatatcgc catcgcgtac tcgatggtcg cggtactgcc
     1021 gccttgcatg gagccatagg gcggaaccat gtttccggca tttctcagaa tgaacagctc
     1081 gcccggctcg gcctgggtca tgagtgtagg atcgacgcga gagtccgaac aggtgatgaa
     1141 gagggctcgg ggttcctgtt tctgcgagag acgtgtaaaa agatctttct ttctcccgaa
     1201 gacctcgccc ctgaatttca gaaatcccct aacgagcttt tccatcgtcg ccttttagcc
     1261 tatcgcccgt aacttgaaat ctcgaccatg agaaccgtca tcagcatcgt ggtgatcagc
     1321 aggcgaaagt gttcatacac caccagaatc aagcgggcta accacttgat cttgacaaga
     1381 tcatccatcg tataggccct gctggttcct ctactcgcga tttctgggaa ctatattccg
     1441 ccacggttgt acggtcaaca cgaatgctgc tatcgggtca ggacaagcca gccccatctg
     1501 ttcctatttc aggagcattc tgcagaaaac catgatcgga atgcagttga taggaaatgt
     1561 tccgagtctg ccactagtat tttctttgct catctcctcg tagaaattct cctgtacgcc
     1621 tgcgatttat ccgagttcct ctccatgagc catggcggta tatgttatgg ggaatcaatt
     1681 cgccactcga gggtggtagt acatcttgaa caagattagg gggccacaat gaagatcttt
     1741 tggctcaacg attctttatg tctgaaaggt gaaaatgatg ccgagagaaa tgcgttagaa
     1801 ctagtacgca ccgcgtttct ccagactact gagatagaaa cagaacaagc ccatggggta
     1861 tgcccctggc taaaccttgt tgagatgact agcgtaagcg tggcccatga agcccaattg
     1921 atctgtaact cattaaccag gatgcagtca ttgttatccg tatatgactt gacccagtcg
     1981 cgaacatgtt aggcagtaga tgatgttcta ggtacccatt gagagtacag gaccttcttt
     2041 cccctccttt gccctctggt tccgtagggc gagataaagg ggttcctaat ttttccctgg
     2101 taatactatg acgtaagagg tgaggtaacg caccatgata tgaaagagca ctaccccgat
     2161 cgccgcgatc cagagggtaa aggcgacatt cagattcgat agtcccaaga tcatttcgca
     2221 gatgtcctgg ggaagtgcct atgatcacca gtgtaggcac aagtatttta cctgctcatt
     2281 tcctcgtata aattctcctg tacacctgtg atttatccga gttcctctcc atgagccatg
     2341 gcggtatatg tatggggaat gaactcgaga acacagctcg tcctcttcgc atgcgacgag
     2401 gtggctttca cggtattcga cattgtcgag tagcagttgc gattgaagaa gatcgatgcg
     2461 gcactcgaca cgatgagcag aattgctgat gcgacagtcc gatcgaagcc actgccatca
     2521 gtcaattcaa ggccagagac cagcggggcg ccctgcagac agcccacccg attccccatg
     2581 aaatcaggtg gagctgggag cgatctgcgc actggcctgc cgtttcaaat ggtctggatg
     2641 catgaaccga tgcgcatgac cttcgtcttc gagggccttc ctgccattgt cagccaaatt
     2701 gtgcaaaaac actgtccgct ccagaaactc ttcgataatc ggtggctgca cctgatcgtg
     2761 ttggacattc ggaccgatca gtttgttcgc tacgagtcgc agggacagtg ggcacctgtg
     2821 tcaatacgtc agccggccct atctacctag ttgtcattga gccggattac acagtagggc
     2881 gaccggcaat tcaggagaga ggccggttgc actacaagcc aaccggattg cctcgatcgg
     2941 tcgtcggggg aagccggttg gcttttctgt tttcttgggt tcctgcgtcg atcatcatga
     3001 ggtaccagtg ttctccatca tcctagttcc attcctcccg ctcctcgcgg cgctcatcgt
     3061 gctcgtcggc gatgagaact cccgacgccg acgggccagg atcgcggcct atcccatcgg
     3121 cgcagctttg ctgggagcca tcgtgaccct ctggtatgtg gcgtcacacg gggccatcac
     3181 cattcggttc tacgacccct cgtcccctgc gacgctggct tttcccattg gtttttatat
     3241 cgatcggctg agtgcgatca tgatggtgtt aatctccggc gtcggaacca tcatctacac
     3301 gtattccgtc ggatacctgt atcaggatcc gcatgaaacc cgataccttt cgctgatcgg
     3361 atttacgacc tccgtactgc tttgcatggt ctcgagtggc aatctcatga tgctgttcat
     3421 cttttggcag ctcctgagtt acctgctcta tatcctggcc cacaatcatg cgcatgcagc
     3481 gacgctggaa ggcgcatttc gaacatttac gcttttgcgc gtcggcgatg tggcgtttct
     3541 ttccggtatc gtcctggccc attcgctcta tggcacattg gagttccagt cgttgttcgt
     3601 caaggcggct gagacgccgg tcacgctttc gcctctgcct ggatgggaac tcaacggagc
     3661 cacagccgtc acgctgctgc tgttcatcgg agggatgagc aagtcggcgc agtttccgat
     3721 gcacgtctgg ctgccccgct cgctctatgc ccccacgccg gtacacgcgt tgctccatgc
     3781 cggcatcatc aatgccggcg ggtttctgat caaccggttg gcgccgctct tcggattgag
     3841 ctcgacaacc ctccacgtag ccttcgccgt cggcaccctg acagctgtcc tcggtgccag
     3901 catgatgctg gcgcaaaacg acatcaagaa aacgctcggt ttctccacaa taggccagat
     3961 gggttacatg atcatggaat gtggattggg cgcgttttct ctcgcagtct tccatctgat
     4021 tgcgcacgga ctgttcaaag cgaccgtgtt tttgaactgt ggcaacgtca tccataaggc
     4081 ccggcaggaa cctcatttcc cccaccatga ccatcaggag aaggaagagg gcttctcccg
     4141 cctcacctgg tcgaccggtt tcgtgacgac gctcttcatt cccctcctga ttctcctcgt
     4201 cacgcacggc gtgttgcata ttccactgct tgagtcgcag ggcacggtga tctttctgtt
     4261 cttcatctgg atcacctcct cgcaggccat catgacactg acacgattga gggccgtggc
     4321 ctcgtggaaa gtgtcgtccg tgatgctgct gaccctgctg ttcgtcgtgt ttgtctatct
     4381 attcgccgtg gaatctttta cggccttcct ctacccgaac ccgcaagagg ttgcgtccta
     4441 tttcaaggcg gccgaactcc cgtattggtt cttcgacctg atgatcatca tggcgacgct
     4501 gacgacgatc gccggatggt tctatctcta catgcgggcc catgggcgga gtgtttggat
     4561 gccgtcgtgg gtcgagggcg tgaggattcg cttgtacgtc ctgttcatga accggcttta
     4621 tgcggacgag gtctatcaaa aagtgggcgg ggtcgccgga cgactcctgg accgattcga
     4681 caaaagcgta cgaggatggt cgcggtgatg gatggtaccg tcgctacatg gctgctggta
     4741 ggtattcccc ttttgggagc agcgttcggc ttcgcggtct ggtcgcgacc gcactatctc
     4801 aggaactggt cggtggtcct gtcgatcctg agcctcgtag cggctgtcag catgtctgga
     4861 tccctcgtga cctcaccgga aaatctgttg ctggtctatc tcctgccggt tgccgcctgt
     4921 gcatctctcc tgggacagcc ggttcacaag aatctgcgac tgtcgtggat caccaccttg
     4981 ctgtttctcg gactgggatt cgtcatcctg acgagccaaa acgtcatagg gcaggtgtgc
     5041 ttgcttgtgc tgctcagttc actcatcgcg ttgctctacc gccaccatac gccattgtgg
     5101 ccaatttcct ggttggggat cggcgcctat gcactcgctg ctgggtgcgt cacgatttcg
     5161 ctcgtggttg ccccgccgct ctcttccgtc gcgtccttgt tggcctgcgc agttttattg
     5221 ccgcttatgc cgtttcacca gggacatatc actgccctta cgcggctgcc cggcggcctg
     5281 ccttcgttca tcgtgttgct cttccccgtg atcggactgc acggcgcaac atcggtcatg
     5341 ccgacagtcc ctgacgcggc agcctggact gtaagcctac ttgccctggc aggcacgttc
     5401 tacggtgcgg ttaaggcgct ggcacaatcg cgtgcccgac tcctcttgtc gtacagcagc
     5461 ctgtcgttct actcgatgct ctggtggttt ggtgcctcga ccaatgccac gacaccacgc
     5521 gctgcggtct tcgtcggggc agtcgggttc accaccagcg gactgctgct cgcttggcag
     5581 gtcatccgaa cgcgctatgg agacgacgtg gacccgcaat ccatcagtgg gttggcagcg
     5641 acgatgccgc gctatgccgt gcttctctcg ctattggcct tggctgcgat aggcctgccg
     5701 ccgttcggcc tctttgccgg tttcatgggc ctacttctgt tgtccaagat ttccttctcc
     5761 atcgcgatcg tcatcatcgt gacggcctgg cttgccgctt catggtacat catgggcatg
     5821 gcgcagcagt ggctcttcgg aacgcgccgc tacgatttgc gctacaccga tctgcttccg
     5881 ggcgaattgg cttcgctcac gatcctcgtt gtggtgttac tggctttggg cgtggtcccg
     5941 acccatctgt ttgaatcggg cacgctcacg gcggcaaccg gcgccatcac ggaattgttt
     6001 gtatggaaca agtagagaac cgacccgaca tcgaagcgag gcgaatggag ttgcgaggga
     6061 ttgtccatct cgcaggcgaa gtcgtcgcgc agtattggcc gatgcggact ttcgtgcacc
     6121 ataacccgct ccatagcctt gaataccttc cgttcgagga gaccgtccag cgcggcaagc
     6181 agttcttggg aggcaacggc tacctgc
//
LOCUS       DATIPF010000365         8538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1299308, whole genome shotgun sequence.
ACCESSION   DATIPF010000365 DATIPF010000000
VERSION     DATIPF010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8538
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299308"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..176
                     /locus_tag="VLL06_09140"
     CDS             48..176
                     /locus_tag="VLL06_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31168.1"
                     /translation="MGELQIHRMELDMQKNELRRAQFDLEVVRNRYVDLYDFPPAG"
     gene            308..1594
                     /gene="pfp"
                     /locus_tag="VLL06_09145"
     CDS             308..1594
                     /gene="pfp"
                     /locus_tag="VLL06_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HSS31169.1"
                     /translation="MHVPRTIVGILVGGGPAPGINSVISAVTIRSILGGCDVVGILDG
                     FKWLMEGSTSQVRPLSIEEVSRIHFRGGSYLGTSRANPTKKAESLANVLSSLTRLGIT
                     RLVTIGGDDTAFSALKLEEQAAGLVHIVHVPKTIDNDLDLPHGIPTFGFQTARHVAVE
                     IVKNLMVDARTTSRWYFAVTMGRKAGHLALGIGKAAGATLTVIPEEFGERPVRLQRLL
                     DLVIGTMIKRLDGGRSDGVVVLAEGLIEILDPQDLGGLDHVERDEHGHLRLADVDIGD
                     VLRREVTKGMKALGLVTSIVAKNVGYELRCADPIPFDMEYTRDLGYCAAQFLFDGGTA
                     AMVSIQNGRFTPIPFKQMVDPVSGRARVRMVDITAQSYQIARQYMIRLSDEDLKDPLV
                     LGRYAALAGLSPEAFHSRFQPVGSNSVTTQLKGCAT"
     gene            2229..3494
                     /locus_tag="VLL06_09150"
     CDS             2229..3494
                     /locus_tag="VLL06_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSS31170.1"
                     /translation="MPARLCNCVVLMTMLVAGTSGMLIPSVVQAKDPMPTFDLDHIID
                     LALERNPMIAGAQSVIAQNEGKRIEAGAYPNPTITVQTADATLRDPSLGRRMAERNMT
                     LSQPLEWPGKRAARQDAAEAGLAGATVGLEESRLNLIADVKTAFYDLLLAERNVDLLQ
                     QNLETVQEVARIVKARVRSGEGPQFEAVKADVEVLKAKQEMTKAKNAVRVKLVGLDTL
                     TAGGLGPRYKVQGDFRSLRDHLDPEQMATRDLSQHPTLKRQRKLVEQAEFTVSKERQS
                     RMPDVTLFGGYAREVGREAVVAGMSLPTPLWYRQQGHIATALGTQRKEEAELIRMRND
                     LARAINQHAREAETAQEQILVYEEGLLKQAQEALRIAQLSFRQGASSLLDVLDAQRVQ
                     RQITVDYNLARFDLSLALTRYERALGGPL"
     gene            3588..4757
                     /locus_tag="VLL06_09155"
     CDS             3588..4757
                     /locus_tag="VLL06_09155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSS31171.1"
                     /translation="MLCSVFSGMLLVAASTGCDRAPAPAEEGKPKHSSDHARLVRLPA
                     DEVTRSGVTVEPVGRTAFRTYRTFPGVVRPNENALANITTLVRGRVAEVHADLGQAVV
                     ANQLLAVLHSSDLGLAQSAYLKARARRHVAEQAYQRAEFLFKEKVIGQAEQQRREGEM
                     ISVRAEAQEAREGIRLLGMDDKEISVLERTQKIRSQISIVAPFAGRVIARDLTKGELV
                     ETTQKLFVVADLSTVWVVGNVSEKDISYMHRATLVPDQPVEVFVPAYPDEVFQGTVAY
                     VGDVLDTATRTMQVRLTLANPNGRLKPEMFATIRVLSEPAPDVLVVPEGAVQHDRDRS
                     FVFVQQQSGVYEARTVKLGDKNGTFVEVREGLQEGEVVVQEGAFTLKSELLKPKD"
     gene            4761..7946
                     /locus_tag="VLL06_09160"
     CDS             4761..7946
                     /locus_tag="VLL06_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HSS31172.1"
                     /translation="MVDKLIELSLRRPFLILTFAGLLTLLGFYAFRTIPIDAFPDVTS
                     VLVQVVTKAPGLSPEEVERLVTFPIEQQITGVPHLTELRSLTKVGLSLITVVFDDRMD
                     INLARQLVLERLIEVQEHLPPGSEPGMVPNSTGLGEVYQYYLEGPSHAGLNAAAVERE
                     LIEQRTVQDWVIRPFLKGTPGVIDVNSQGGFVKQFQVLVEPELLHKYSLSLDDVFTAV
                     ANNNANAAGNILETHAEKFIVRGVGLIRKLSDIEDIVVKEAGGTPVHIHDVAQVQIGH
                     AIRHGAVVLNGEREVVAGIVLMLRGGNARDVVEGIKGKIAQIQDKGMLPVGWRIVPFY
                     DRIELISAALKTVYKALAEGILLVVVVLFVFLGDTRSALIVAATLTVTPLVTFFVMDR
                     FDLTANLMSLGGLAIAIGMMVDASVVVVENIHRHLSDPRHQGLPRLALILNAAREVAR
                     PVVFGIIIIIIVFMPILSFEGMEGKMFKPMAYTIMIALMVSLILSVTLSPVLCLLTLR
                     PGHGADPWALRLAKRIYIPLLRWALGHRAVVLTATGLMLAGSIALFPFLGSEFVPILN
                     EGTMAPLTIRLPSISLEQSITIEREVQKAVLEFPEVQMMVSKIGRTELANDPQEANES
                     DSVAMLHPIETWTTASTMEGLKERVRERLARVPGANFLISQPIQQRVDELISGVRAEV
                     TVKLFGPDLETLRHTGDEIAGVLRTIRGVKDLKVEQLFGQPYITIDIDRSKIARHGIN
                     VSDVRDVIATAVGGESATRVYEEQRRFNLIVRFPEQYRNSIETIGNIRMSDGKGAFIP
                     LSDLGKIRMHEGPGRINRDQLQRYLPVSFNTHGRDIGSIVAEAQQRMAREVRIPEGYA
                     VLWGGSFENMERAMARIKIIVPITIGVIFLLLFSSFSSLKQAALIILNLPFALIGGII
                     ALWITGEYLSVPASVGFINLFGVAVLNGIVLVSYMNALRRQEGMDVRQAVLNGCVMRL
                     RPVLMTAAVALLGLVPLALSQGIGSEVQRPLAVVVIGGLVSSTFLTLVVLPVLYQWIE
                     ERTAVPSLSPSVANGEGHAMPDRHEEVKLTS"
     gene            8139..>8538
                     /locus_tag="VLL06_09165"
     CDS             8139..>8538
                     /locus_tag="VLL06_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31173.1"
                     /translation="MSDHKVEPHVFVIIGATGDLTRRKLLPALYDLRDQGVLDTRNTL
                     IIGAAVSEMSEEEFRLWAIEGLQQAGCGEEAELRAWCEECLHYQSVRKGGVQYYGSLA
                     KYIRRLERKHHMPENRVFYLALPPDVVPIAM"
BASE COUNT         1739 a         2297 c         2622 g         1880 t
ORIGIN      
        1 gccgcgcgca ttgctgggtc acgccggtca aggatgtgta acagctcgtg ggtgagttgc
       61 aaatccatcg gatggagctg gatatgcaaa agaacgagtt gcgccgagcc cagttcgacc
      121 tcgaagttgt gcgtaaccgc tatgtggacc tctacgattt tcccccggcg ggctgatcta
      181 cctccagttg ttaggcctcc ggttcagttc gttcacacca agtccttggc gggaccatcc
      241 cagtcgcgtt agtctatcgg tcagtgtact cgagacaggt ggattgcatc acaggagttc
      301 tcacattatg catgttcctc gcacgatcgt cggtatctta gtcgggggcg gaccggctcc
      361 cggcatcaac agcgtcatca gtgccgtcac gatccgcagt atcctcggcg gttgcgatgt
      421 cgtcggcatc ctcgacggat tcaaatggct gatggaaggg agcaccagcc aggtgcgccc
      481 tctctcgatc gaagaagtca gtcgcattca ttttcgaggc gggtcgtatc tgggcacctc
      541 gcgggcaaac ccgacgaaga aggcggagtc tctcgccaac gtcctgtcct ccttgacgag
      601 gctcggcatc acgcgcctgg tgaccatcgg cggtgatgat acggccttct cggcgctcaa
      661 attggaagaa caggcggccg ggctcgttca catcgtgcat gtgccgaaga ccatcgacaa
      721 tgacctcgat cttccccatg gcattccgac attcggcttt caaacggcca ggcatgtcgc
      781 cgtcgagatc gtgaagaatc tcatggtgga tgcccgcacc acatcgcgct ggtacttcgc
      841 ggtcaccatg gggcgcaaag ccggccattt ggcgctggga atcgggaagg ccgccggtgc
      901 cacgctcacc gttattcccg aggaatttgg agagcggccc gtcagactcc agcgcttgtt
      961 ggatctcgtc atcgggacga tgatcaagcg tctcgacgga gggcgctccg acggggtggt
     1021 cgtgttagcg gaaggactga tcgagattct tgatccccag gacctagggg gattggacca
     1081 cgtcgagcgg gatgaacatg gccacctgcg attggcagat gtggacatcg gcgatgtgct
     1141 ccggcgtgaa gtgacgaagg gcatgaaagc cttgggactt gtcacgtcga tcgtcgccaa
     1201 gaacgtgggg tatgagttac gttgtgcgga tccgattccg ttcgatatgg aatacactag
     1261 ggatctcggc tattgcgcgg cgcagtttct tttcgatgga ggcacggcgg ccatggtatc
     1321 gattcaaaat gggcggttca cgccgattcc tttcaagcag atggtggatc ccgtcagcgg
     1381 ccgggcaaga gtgcggatgg tagatatcac ggcacagtcg taccagattg cgcgtcagta
     1441 catgattcgg ttgtccgatg aggacctcaa ggatccgctg gttctaggtc gctatgcggc
     1501 tctcgcggga ttgtctccgg aggcgtttca cagccggttt cagcctgtcg gatcgaactc
     1561 ggtgaccact caattaaaag gttgtgcaac atgaactatg ctcggggcga cgattgggtc
     1621 taaacatggg cgatggggct cgagctgggt ggagggcaac gaacgatcat gaataatgcg
     1681 ggctaacggt ttttcttgta accgaggcct agagagatgt actatgggag tgattgtttc
     1741 gactgtgtca caaccgccat gggggctgtt cagaacatgt cagcgaacgt atcagcagcc
     1801 ttgccgtatt gatgggaagc gttgtggcta tcatccgttc atgtttctct gtcctcgtct
     1861 ttgcgttgct tctctgcagc gtattcccag tcgagcaggt caggtgggct gccgatagca
     1921 tcgtttgtgg ttcggccgga aagactggac atgagcaggc ttgcgattcc agggataccg
     1981 cggcggcgac cgagtccgga gacgattcgt cgcaagagat gcaggacgac tatgtcactt
     2041 ctggagtgag tgtgtcagat tgcgggtcga gccgtacgca tgcctcgatg aacgaaaatc
     2101 ctccttccac ccttctggtc tttcaactct tacatcctcc gaccgtacag agctagcacc
     2161 acgtcgacgg gctattcatt ttcgatctcg acgggacaac ttcattgtga ctccgaagga
     2221 ggcatcacat gccggcgcgg ctatgtaatt gtgtcgtatt gatgacgatg ttggtagcag
     2281 gaacaagtgg gatgttgatc ccgtctgttg tccaggcgaa agacccaatg cccacgttcg
     2341 atctcgacca catcatcgac ctggcgctgg aacgaaaccc gatgatcgcg ggcgcccaga
     2401 gtgtgatcgc acagaatgag ggcaagcgga tagaagccgg tgcctatccc aatcccacga
     2461 tcactgtcca gacggctgac gcgacacttc gagaccccag cctcgggagg cgtatggccg
     2521 agcgcaacat gacgttgagt cagccgctgg aatggccggg caagcgggcg gcccgtcagg
     2581 atgcggccga ggccggttta gccggcgcta cggtcggcct cgaagaatcc aggctcaatc
     2641 tcatcgcgga tgtgaagacg gcgttctatg acttgctgct ggcagaacgg aatgtcgacc
     2701 tgcttcagca gaatttggag actgttcaag aggtggcgcg cattgtcaag gcccgcgtcc
     2761 gatcaggcga aggcccgcaa ttcgaagcgg tgaaggcgga tgtggaggtg ctcaaggcca
     2821 agcaggagat gaccaaagcc aagaacgccg tgcgcgtgaa actcgtcgga ttggacacct
     2881 tgacagcggg agggttgggg cctcggtaca aggtgcaagg ggatttccgg tcactccgag
     2941 atcatctgga cccggagcag atggcgacgc gggacctttc gcaacatccc actctgaaac
     3001 gtcaacgcaa actggtggag caggctgagt ttactgtcag caaggaacgg cagagccgta
     3061 tgccggacgt caccctgttc ggcggatatg ccagagaagt aggccgcgag gcggtggtgg
     3121 cgggcatgag tcttccgacg cccttatggt atcggcagca aggacacatt gccacggcac
     3181 tcgggactca acgaaaagaa gaggcggagt tgattcgaat gagaaacgac ctggcccgcg
     3241 ccatcaatca acatgcgcga gaggcggaaa cggcacaaga gcagattctc gtatatgagg
     3301 aagggctcct caagcaagcg caagaagcgc ttcgtattgc ccaattgagt ttcaggcagg
     3361 gcgcctccag cctcctggat gtcctcgatg cccaacgggt ccaacgccag atcaccgtgg
     3421 attataatct ggcacgtttc gacctttccc tggcgctcac gcgatacgag cgagccctgg
     3481 gcggtccact ctagtggtga agggtacaga tgagacgcat ggcacttttt cctgaccgaa
     3541 tgatgacacg acgcagctgt cacacgcatc cgcgaagccg tctgagtatg ctgtgcagcg
     3601 tcttctccgg catgctgttg gtggcagcct cgactggctg cgaccgtgcc cctgccccgg
     3661 cggaggaggg caagccgaaa cattcgtcgg accatgcacg ccttgttcgg ctcccggcag
     3721 atgaagtcac tcggtccggc gtgaccgtgg agccggtcgg gcgaacggcc tttcgtacct
     3781 accggacctt tcccggcgtc gtgcgtccga acgaaaacgc gctggcgaac attacgaccc
     3841 tcgtccgtgg gcgagtggcc gaggtgcatg ccgatctggg acaggcagtc gtggccaatc
     3901 agctgctggc agtgcttcac agcagcgatc tcggactcgc ccagtctgca tacttgaagg
     3961 ctcgtgcgcg acgacatgtg gcggagcagg cctaccagcg ggcggaattt ctatttaaag
     4021 agaaggtcat cggccaggcg gaacagcagc gccgcgaagg agaaatgatc agtgtgcgtg
     4081 ctgaagctca ggaggcgcgc gaaggtatcc ggttgctcgg catggacgac aaggagatca
     4141 gtgtactaga acggacccaa aagatccgct cgcagatttc cattgtggcg ccttttgcgg
     4201 gtagggtgat cgcgcgtgat ctcaccaaag gcgagctggt cgaaaccacc caaaaactgt
     4261 ttgtggtcgc cgatctttcg acggtctggg tcgtgggcaa tgtctcggag aaggatatct
     4321 cctatatgca tcgggcaacg ctcgtgccgg atcagccggt cgaggtcttt gtccctgcgt
     4381 atcctgacga agtgttccag ggaacggttg cgtatgtcgg cgatgttctg gacaccgcga
     4441 cgcggaccat gcaggtgcgc ctcactttgg caaatcccaa cggtcgtctg aagccggaaa
     4501 tgttcgcgac aatccgcgtc ctatccgaac ctgctccgga cgttctggtc gttcccgaag
     4561 gagccgtgca gcatgatcgg gaccgcagct tcgtgtttgt gcagcagcaa tccggagtct
     4621 atgaagcacg gacagtcaaa ttgggcgaca agaacgggac cttcgtcgag gtccgcgaag
     4681 ggttgcagga aggggaagtg gtcgtccagg aaggcgcctt taccttgaaa tccgagttgt
     4741 tgaagcccaa agactgaact atggtcgata aactcatcga gttgtctctg cgtcgcccct
     4801 tcctgatcct tacgttcgcc ggccttctga cccttctggg gttctatgcc ttccgcacga
     4861 taccgatcga cgcatttccc gatgtgacaa gcgtgctcgt gcaagttgtc acgaaagcgc
     4921 ctggcttatc ccccgaagaa gtcgagcggc tggtgacctt tcccatcgaa caacaaatca
     4981 ccggagttcc gcatttgacg gaactccgtt cgctcaccaa agtcggactg tctctcatta
     5041 cggtggtgtt cgacgatagg atggacatca accttgcccg ccaactcgtg ctcgaacggt
     5101 tgatcgaggt gcaggaacac ttacctcctg ggtctgaacc gggcatggtg ccgaacagta
     5161 cgggactggg cgaggtctat cagtactatc tcgaaggccc ttctcatgcc ggtctgaacg
     5221 ctgccgctgt ggaacgtgaa ttgatcgaac agcgaacggt gcaggattgg gtcatccggc
     5281 cgttcctcaa gggcacccct ggagtgatcg acgtcaactc gcaaggcggg tttgtcaaac
     5341 agttccaggt gctcgttgag ccggagctgt tgcacaaata ttcgctctct ctggacgacg
     5401 tctttacggc cgtggcgaac aacaacgcga atgccgcagg caatatattg gagacccatg
     5461 ccgaaaagtt cattgtccgc ggtgtgggac tgattcggaa actgagcgac atcgaggaca
     5521 ttgtcgtgaa ggaagcgggt ggtactcccg tacacatcca tgacgttgcc caggtacaaa
     5581 tcggccatgc cattcgtcat ggcgctgtcg tcctgaacgg tgagcgtgaa gtggtggcag
     5641 ggatcgtact catgctgcgc gggggaaatg cccgtgacgt cgtagaaggc attaagggca
     5701 agatcgccca gatccaggac aagggtatgt tgccggtagg atggcggatc gtgccatttt
     5761 acgatcgcat cgaattgatc tccgccgcgt tgaagaccgt ctataaggcc ctggccgagg
     5821 gtattctcct ggtggtcgtg gtcttatttg tatttcttgg cgataccagg agcgcattga
     5881 tcgtggcggc gacgctcacc gtcacgcccc tcgtgacgtt ctttgtgatg gatcggttcg
     5941 atctgacggc caatctgatg tctttggggg gtctggccat tgccatcggc atgatggtcg
     6001 atgcctctgt ggtagtggta gaaaacatcc accggcacct ctcggatccc cgccaccagg
     6061 gcctgccccg attggcgctc atcctcaatg ccgcccggga ggttgcccgc cccgtggtct
     6121 tcggaattat cattattatt attgtcttta tgcccatcct ctcgttcgag ggtatggaag
     6181 gcaaaatgtt caagccgatg gcctatacca tcatgattgc cctgatggtg tcgctcattc
     6241 tgtcggtcac cctgtcgccg gttctgtgtt tgctgacgtt gcggccaggg cacggcgccg
     6301 atccctgggc gcttcgactg gccaagcgga tctatattcc tttgctgcgc tgggcgcttg
     6361 gacatcgggc ggtggttctg acggccactg gactcatgct ggcgggaagc attgcgctct
     6421 ttcctttttt ggggtctgaa tttgtcccca ttctgaatga aggcacgatg gctcccctta
     6481 ctatccgttt gccgagcatt tctttggagc aatcgatcac gatcgaacgc gaggtgcaga
     6541 aagcggtgct ggagtttcca gaagtgcaga tgatggtgtc caagatcgga cgcacggaat
     6601 tggcgaatga tccgcaagaa gcgaatgaaa gcgattccgt tgcgatgttg catccgatcg
     6661 agacatggac gacagcctcc accatggagg gattgaaaga gcgagtccgt gaacggctgg
     6721 cgcgggtgcc cggcgccaac tttctgatca gccagccgat ccaacaacgg gtagacgaat
     6781 tgatttcggg ggtgcgcgca gaggtgaccg ttaaattgtt cgggcccgat ttggagacgc
     6841 tgcgtcacac gggggatgag attgccggcg ttctccgcac gattcgaggt gtcaaagact
     6901 tgaaggtgga gcaactcttc ggccagccct atatcacgat cgatatcgac cgaagcaaga
     6961 ttgcgcggca tgggatcaac gtctcggatg tgcgcgatgt cattgccacc gcggtcggag
     7021 gtgaatcggc gacccgggtc tacgaagagc agcgacggtt caatctcatt gtgcgatttc
     7081 cggaacaata tcggaatagc attgagacca tcgggaacat tcgaatgtcg gacggcaagg
     7141 gggccttcat tccactcagc gatctcggca agatccggat gcatgaggga ccgggccgta
     7201 tcaaccgcga ccaacttcag cggtacctgc cggtcagctt taatacccac ggccgggaca
     7261 tcggaagtat tgtcgccgag gcgcagcaac ggatggctcg ggaggtccgg attccggagg
     7321 ggtatgcggt cctgtggggc ggatcgttcg aaaatatgga gcgggcgatg gctcgcatca
     7381 agatcattgt tccgattacc atcggggtga ttttcttgct cctgttctcg tcgttctcct
     7441 cgctcaagca ggcggcattg atcattctca acctcccgtt tgccctcatc ggcgggatta
     7501 tcgcgttatg gattacgggt gaatatctga gcgtgccggc ctcggtcgga ttcatcaatc
     7561 tgttcggtgt ggccgtcttg aacggcatcg tgttggtgtc ttacatgaat gcgcttcgtc
     7621 ggcaggaggg catggatgtt cggcaggcgg tgttgaatgg atgcgtcatg cgtctccgtc
     7681 ccgtcttgat gacggcggcc gtcgccctac tggggttggt gccgcttgcc ctttcccagg
     7741 ggatcggatc ggaagttcaa cggcctctgg ctgtcgtggt catcggggga ctcgtcagtt
     7801 cgacgttcct gactctcgta gtactgccgg tgctttatca gtggatcgaa gaacggactg
     7861 ccgtcccgag cctctcgccg agcgtcgcaa acggagaggg acatgcgatg ccggatcgtc
     7921 acgaggaggt gaagctgacc tcttgatcac gagacagcgc gcaaaaatgg ttttcgcata
     7981 ataccgctct cttcattcct cgttcctccc tcatttcaca accgcttgac atattccagc
     8041 gtgcgctcca agcttcacgg atgtcgatcg tgctgttggc ggattgattc agcatcctgc
     8101 tcacggattg ttcaccgttt atacggaggg ccttgcgaat gtcggaccat aaggttgagc
     8161 cccatgtctt tgtcattatc ggtgcgaccg gagatctgac gcgccggaag ctcctccccg
     8221 cgctctatga tttgcgagat cagggcgtcc tggacactcg caacacgttg atcatcggcg
     8281 ccgctgtgtc tgagatgagt gaagaggaat tccggctctg ggccatcgaa ggtctacagc
     8341 aagcgggctg cggcgaggag gcagagctgc gtgcctggtg cgaggagtgc cttcactacc
     8401 agagcgtgcg taaaggggga gtgcagtact atgggtcgtt ggccaagtat atccgacggt
     8461 tggaacgcaa gcaccacatg ccggagaacc gggtattcta cttggcattg ccaccggacg
     8521 ttgtgcccat cgccatgg
//
LOCUS       DATIPF010000366         6733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1748591, whole genome shotgun sequence.
ACCESSION   DATIPF010000366 DATIPF010000000
VERSION     DATIPF010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748591"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..423)
                     /locus_tag="VLL06_09170"
     CDS             complement(40..423)
                     /locus_tag="VLL06_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31174.1"
                     /translation="MDSASGSCNACGATGIALMKVSLGKDFFGRAYDRLSPSTDQSPK
                     WYCEGCSMQKNLQRDFRDVLGEFDKLTAGQGSILAAPEEFQRASLRLREISTILAGVT
                     GHSSFLTAADVTQLIGRMQTTTMQA"
     gene            599..1702
                     /gene="ald"
                     /locus_tag="VLL06_09175"
     CDS             599..1702
                     /gene="ald"
                     /locus_tag="VLL06_09175"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HSS31175.1"
                     /translation="MVIGVPKEIKDHEYRVSVTPNGVRALRQAGHDIWVEPSAGLGSG
                     YSDEDYRGAGATMADSKEQLFQNATLIVKVKEPLLSECHFFRPDQTLFTYLHLASLPD
                     LTKTLLETTITAIAYETIEAKDGTLPMLKPMSEIAGRMSVQIGARYLEKTQGGRGLLL
                     AGVPGVDPGKVVVLGGGVVGSSAIRIAVGMGAQVTVINLDLERLRALDDLYRGRIVTR
                     ASSQAAIDDSVMAADLVIGAVLVPGARAPKLVSRELVARMKPGAVVVDVAVDQGGCFE
                     TTKPTTHSDPVYVVNGVLHYCVANMPGIVPHTSTIALTNATLPYLLRLATQGIASAVR
                     ADAGLAKGVNLSGGKITCRGVADAHGLRFDPLM"
     gene            1741..1817
                     /locus_tag="VLL06_09180"
     tRNA            1741..1817
                     /locus_tag="VLL06_09180"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1775..1777,aa:Pro,seq:cgg)
     gene            2194..2490
                     /locus_tag="VLL06_09185"
     CDS             2194..2490
                     /locus_tag="VLL06_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HSS31176.1"
                     /translation="MTEPIEAATLRARLFVKGKVQGVGYRAFAARIASQRGLCGGVRN
                     LDDGRVELDVEGPKDQILILIEDAKTGPPASRVAAVDVEWSPATGRFSDFQVWY"
     gene            2644..3612
                     /locus_tag="VLL06_09190"
     CDS             2644..3612
                     /locus_tag="VLL06_09190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSS31177.1"
                     /translation="MARGGEELHDLGEARRHVVDHDDEDAAASPGVDESLAGEEKPSR
                     RSEGLDTLKCYLREIRHSTLLTFKQEQQLGKRVMAGDEQARQEMIESNLRLVISIGKR
                     YMHRGFPFSDVIEEGNLGLIKAVEKFNYKRGFRFSTYASWWIRQYIERAIINQGKLVR
                     LPVHVVERLNRYLAKAEQLVQELGREPTASEVAARMKTTDETVLDLKQVVRTTCSLDS
                     PINEGGDTFLRDVIEDPSCASPADTAEGVMRRAEMMEWVQELPEKEQIVIMSRFGLDG
                     SEAKTLEEIGREMGLTRERVRQIEMAALARLRHTIERRTMTQADLL"
     gene            3616..4140
                     /locus_tag="VLL06_09195"
     CDS             3616..4140
                     /locus_tag="VLL06_09195"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HSS31178.1"
                     /translation="MTAANYRTLIREVPDFPKPGILFYDITTLLKDPKALSSIADELT
                     AYYQGQRIAKVIGIESRGFIFGGVLANRLNAGFVPVRKPGKLPADSFEVKYSLEYGAN
                     SLAIHRDAIAMGERVLIVDDLLATGGTAAATVSLVRQLGGEIAGLDFLVELQGLKGRA
                     KLVGYPIHSTILYP"
     gene            4239..4805
                     /locus_tag="VLL06_09200"
     CDS             4239..4805
                     /locus_tag="VLL06_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HSS31179.1"
                     /translation="MATKTPAKKKPVAKAKVRATLAKPASKPKRAPVVIVEKPISMAV
                     SPLAAKPKESAKEREDRERRRQVLHQMLMRKRQDIIKEIEGSLGQSLIEDKQRRLESA
                     RDVGDQALMDLDRELGISLMEMRNRRRQAIDEALTRLTEGTYGICAECGVEISERRLE
                     AVPFAKLCVECQSKEELLERIEREEDRD"
     gene            4950..5654
                     /gene="queC"
                     /locus_tag="VLL06_09205"
     CDS             4950..5654
                     /gene="queC"
                     /locus_tag="VLL06_09205"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HSS31180.1"
                     /translation="MNPTSHRAVVLASGGLDSTVTAALAKQEGLELFFLTIAYGQRHG
                     VEVERARQIAAALGVTNHLVMNLDLRAIGGSALTGPAAVPKDRADNERSQSIPATYVP
                     GRNLIFLSIAAAHAEVVGAAFIYFGANVLDYSGYPDCRPEFIRAFESAVKEGTKAGTE
                     GSSLQVKAPLLMLTKAEIIRQGLALHVPFHLTHSCYDPVGDRACGRCDSCVIRREGFA
                     KAGVVDPIPYAITSGC"
     gene            5904..6299
                     /locus_tag="VLL06_09210"
     CDS             5904..6299
                     /locus_tag="VLL06_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSS31181.1"
                     /translation="MMTSKLQIGVCLLGLGLLVACDSTQPTQPTQAAVSGAVPAEFQT
                     GEAIFNANCSACHGKQAAGTDHGPPLVHKIYEPNHHGDPAFLRAATNGVTAHHWQFGN
                     MPKIESVTAGDVDQIVKYVRWLQRQAGIQ"
     regulatory      6441..6545
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
BASE COUNT         1500 a         1797 c         1966 g         1470 t
ORIGIN      
        1 gaaaatatgt cgggtgaatg tcgacaagaa tggtccctac taggcctgca tcgtcgtggt
       61 ttgcatacga ccgatcaatt gcgtgacgtc ggctgcagtc aagaatgaag agtggcccgt
      121 cacaccggcc agaatcgtcg agatctctcg cagccgtaac gaggcccgtt gaaattcttc
      181 cggggcagcc aggatcgaac cttggccagc cgtgagttta tcgaattccc ccaggacatc
      241 ccgaaagtcc cgctgaagat ttttctgcat cgagcaaccc tcgcagtacc atttagggct
      301 ttgatcggtc gagggggaca gccggtcata ggctcgaccg aaaaaatcct ttccgagcga
      361 taccttcatg agcgcgatgc cggtggcgcc gcaggcattg caggacccag acgctgaatc
      421 catagagcct ccctctgtcg atcgtatgct gaagagcggg cgaatcttac actaggcctg
      481 tgcgcactgt ccacttgctg aaaacatggc tgagggccaa atcgtgcatt ttcaccgccg
      541 ttgtagatag aatgaatttg agaattgact ggacggattg ttcaaggagg aaatggccat
      601 ggtcattggg gtccccaaag agatcaagga tcatgagtat cgtgtcagtg tgacgcccaa
      661 tggagtgcgg gcgttgcgtc aggccggcca tgacatctgg gttgaaccat cggctggact
      721 cggcagcggg tactcagatg aggactaccg aggtgccggc gcgaccatgg cagactcaaa
      781 agagcaattg tttcagaatg cgaccctcat tgtgaaggtc aaagagccgc tactttccga
      841 atgtcacttc ttccgtccgg accagaccct gtttacctat ctgcatttgg cttccttgcc
      901 ggatctcacg aagaccctcc tggagaccac gatcactgcg attgcctatg agacgatcga
      961 ggcgaaagac ggcacgttgc cgatgctcaa gccaatgagt gaaatcgcag gtcggatgtc
     1021 agtgcagatc ggcgcacgct atctggaaaa gactcaagga gggcgcggac ttctcttggc
     1081 tggcgtgccc ggtgtcgatc cggggaaggt cgtggtgctt gggggtgggg tggtcggtag
     1141 ttcggcgatc cggattgcag tcgggatggg ggctcaggtg accgtgatca atctcgacct
     1201 tgaacggctc cgggcgctcg atgatttgta tcgtggtcga atcgtcacgc gcgcctcctc
     1261 gcaagctgca atagatgatt cagtgatggc cgcagatctt gtcatcggag cagttctcgt
     1321 tcctggggct cgagccccaa agttagtgtc gcgtgaactg gtggcacgca tgaagccggg
     1381 agccgtggtg gtggacgtcg cggtcgatca aggcggctgc ttcgagacca caaaacccac
     1441 gacccattca gacccggtct atgtggtgaa cggggtgcta cattattgtg tggccaatat
     1501 gccgggaatt gttccacata cgtctacgat cgcattgacg aatgccacgc ttccatatct
     1561 tctgcgtctg gctactcagg gaatagcctc tgccgtgcgg gccgacgctg gattggccaa
     1621 aggggtaaac ctgtctgggg gcaaaattac ctgtcgaggc gtggccgatg cccacggctt
     1681 gcgtttcgac cctctgatgt aagacaatcc cacctcgctt tgtcgttctg ttgattttgt
     1741 cggggcgtag cgcagcccgg tagcgcactg cgttcgggac gcaggggtcg gaggttcgaa
     1801 tcctctcgcc ccgaccaaac caaacatgct cggcccggag cctattcttc tctcgagacg
     1861 gcacgatacc tctgcagggt tctttcctcc agctagttgc agactcttac gaccttcgac
     1921 atatcttggg tttctctcag acagcctata tacctgccaa tcttctcagg ggtcacttcc
     1981 acgggataac taccccactc tgattacgct gcgctcatca tgcggtgcgg aacactcgcc
     2041 ccagcagtta cacgtgatgt ccattttcca tcccggatcc gttgtacgaa attctaccgc
     2101 gaggaatcgc aaaacgcgtg acgcctctgc ggaggtatga gaatatccct tcgttggaca
     2161 gtcaccattg taatgcaggg attgcctgag tgtatgaccg aaccgattga agcggcaact
     2221 ctccgagcaa gactgtttgt gaaggggaag gtccaaggag ttgggtatcg agcgtttgcg
     2281 gctcggatcg catcacaacg gggactctgc ggaggtgtca ggaatctcga tgatggccga
     2341 gtcgaacttg acgttgaggg ccccaaagac cagatcctga ttctgattga agacgcaaag
     2401 actgggcctc ctgcatcccg agtggcagcc gttgatgttg agtggagtcc ggcaacaggg
     2461 cgattttcag attttcaagt gtggtactga aggagttgat ttgcagggtg ctcaaaaagg
     2521 ctgtccagca cggccgcagt gagcgaagag gcgaggcgta cacttcagta cgttgagcct
     2581 ctgagcgaag cgagaacgaa gctggcggac tttttcagca ccctgtcagg agaaacggaa
     2641 cgtatggccc gaggaggcga ggaattacac gatttgggtg aggcgcgccg acacgtcgtc
     2701 gatcatgacg acgaggacgc ggccgcatcg ccgggagtgg acgaatccct cgccggagag
     2761 gagaaaccga gtcgccgttc ggaaggattg gacacgctca agtgctactt gcgcgagatc
     2821 cgtcattcga ccttgctgac gtttaagcaa gaacagcaac tgggcaagcg ggtcatggcg
     2881 ggggacgaac aggcgcgtca ggagatgatc gaatcgaatc tccgtctggt catcagtatc
     2941 ggtaagcggt atatgcaccg cgggttcccc ttttccgatg tcatcgaaga gggcaatctc
     3001 ggacttatca aggcagttga aaaattcaac tataagcggg ggtttcgctt cagcacctat
     3061 gcctcctggt ggatccggca atatatcgaa cgcgcgatta tcaatcaagg aaagctcgtg
     3121 cggttgccgg tccatgtggt cgagcggctg aaccgttatc tcgccaaggc agagcagctc
     3181 gttcaggagc tcgggcggga accgaccgcg tcggaagtgg cggcacggat gaagacaacg
     3241 gacgaaacag tgttggatct caagcaagta gtccgtacga cctgttcgct ggatagtccg
     3301 attaacgagg gcggagatac attcttgcgg gatgtcattg aagacccttc gtgtgcctcg
     3361 cctgccgata cggccgaagg ggtgatgcgt cgggcggaga tgatggaatg ggtgcaggag
     3421 ttgccagaga aggagcaaat cgttatcatg tcacggtttg ggctcgatgg gagcgaggcc
     3481 aagacgctag aagaaatcgg ccgggaaatg gggttgaccc gtgagcgggt ccggcagatt
     3541 gaaatggccg ccttggcgcg attgcgccat accattgaac gcagaaccat gacacaagcc
     3601 gacttgctct gatcaatgac cgccgccaat tatcgcactc tcattcgcga agtccccgac
     3661 ttccccaagc caggtattct cttctacgac atcactaccc tgctcaaaga cccgaaggcc
     3721 ttgtcctcga ttgccgatga attgactgcc tactaccagg gacagcggat cgccaaagtc
     3781 atcggcattg agtcgcgcgg gttcattttc gggggggtgc tggcgaaccg gttgaacgcc
     3841 gggtttgtac cagtccggaa acccggcaag ctgccggccg atagttttga agtcaaatac
     3901 agcctcgaat acggtgcaaa ttcccttgcg atccaccgcg atgcgattgc gatgggagaa
     3961 cgggtactga tcgtggacga cttactggcc acagggggca cagcggccgc gacggtttct
     4021 ctggttcggc agttgggagg cgagatcgcc gggctcgact ttctcgtaga actccaaggt
     4081 cttaagggtc gagcaaaact ggtcggctac cccatccatt cgacaatcct ctatccctag
     4141 cctcttcccc acaacccctt gtcaatgatc atggcagcat gttatgaatg ccgaactttt
     4201 ccggcgcttc gacgttcacc aaaggaatag gatctgccat ggcaacaaaa acacccgcca
     4261 agaagaaacc agtggcgaag gccaaggtgc gggcgacgct cgcaaaacca gcgagtaaac
     4321 caaagcgcgc tccagtcgtg atcgtcgaaa agcctatcag catggctgtc tctcccctgg
     4381 ctgccaaacc gaaggagtct gcgaaagaac gcgaagaccg ggaacgccgc cgccaagtcc
     4441 tccatcagat gttgatgcgg aagcgccagg acattatcaa agagattgag gggagcctgg
     4501 gacagtccct cattgaagat aagcagcggc ggcttgaatc ggcacgagac gtcggtgatc
     4561 aagccctcat ggatctcgat cgcgagctag gcatctcctt gatggaaatg cgcaatcgac
     4621 gccggcaggc catcgatgaa gcgttaacga ggctcaccga aggcacttat ggtatctgcg
     4681 cggagtgtgg cgtagagatc agtgagcgga gattggaagc ggtcccgttc gcgaagctct
     4741 gcgtcgagtg tcagtcgaaa gaagagctcc tcgaaagaat cgagcgagaa gaagatcggg
     4801 attaggcgtt cctcctcgac atcggttcta tccctccccc cccacagagt cgcatcccgt
     4861 ttatcataac ctttgctcgt tcttcgtcgg gcaaggctcg cgttctgtgg cgatgattga
     4921 tatcctgcgt caatacagga gttcgtgtga tgaatccgac gagtcatcga gcagtcgtgt
     4981 tggcgagcgg aggactggat tccaccgtca cagcagcgct ggccaagcag gaggggttgg
     5041 agcttttctt cctcacgatc gcctacgggc agcgccatgg cgtggaggta gagcgggctc
     5101 ggcaaatcgc agctgctttg ggcgtcacca atcatctggt aatgaatctg gatcttcgcg
     5161 cgataggagg atcggccctc actggtccgg cggcagtgcc caaggatcga gccgacaacg
     5221 aacgcagcca gagcattcct gccacctatg taccgggacg gaatctcatc tttctctcga
     5281 ttgcagcagc acatgcggaa gtcgtgggag cagcattcat ctattttggc gcaaacgtat
     5341 tggattattc cggctatcca gactgccggc ctgaattcat tcgcgccttt gagtcggcgg
     5401 tgaaggaagg cacaaaagcc ggtacagaag ggagctccct ccaggtcaaa gctcccttgc
     5461 tgatgctgac gaaagcagag attatccggc aagggctcgc tctgcatgtc ccgtttcatt
     5521 tgacccatag ctgttatgat ccggtgggag atcgggcttg tggacgatgc gatagttgcg
     5581 tgatccggcg ggagggattt gcgaaggccg gggttgtgga ccctatccca tatgcgataa
     5641 cctcaggatg ttgaaacagg cggccagcgg cgttctcgac ccatcgaaat cttcaacgta
     5701 ccggttgaga ttttcagagg tcggaagtgc tggggggggg attccgttcg ctaagatcca
     5761 ttgtgcgggc gaacggcccc acggagtgcg gtcggtacct cctctggttt cgattcgcct
     5821 gcggccttgc tgaccatcct gtttgaacat tctatagtga cattcaattg aacgagatga
     5881 gcggtcctga ttctaggaaa atcatgatga cgtcaaaact tcagatcgga gtgtgtttgc
     5941 taggcctggg cctcctcgtg gcctgcgact ccactcaacc cactcaaccc actcaagcgg
     6001 ccgtgagtgg cgctgtgccg gctgagttcc aaacgggcga agccatattc aacgcaaact
     6061 gttcggcctg tcatgggaaa caagcagcgg gaaccgacca cggacctccg ttagttcaca
     6121 aaatttatga accgaaccat cacggagatc cggcctttct acgtgctgcg accaatggcg
     6181 tcacagcgca ccattggcag tttggcaaca tgccgaagat cgaaagcgtg accgccgggg
     6241 atgtcgatca gatcgtgaag tatgtgcgtt ggcttcaacg ccaggcaggc attcaataga
     6301 tcttctttct cctttcattt ttcacgtgta acgtttccaa ccctcccgcc tttcgcgtcc
     6361 atacctctcc tctcgtgcag ctccatctgc catgcggaag attttcgtga cttttccgcc
     6421 gcatggtcgt ataatcatgt cgaaggcgaa actagggttc cgattgcctt ttcaggcaat
     6481 gtctggtcca agagtttccg gtctcgcatg gcgaggctcc acggagggat aaaagcccgg
     6541 gagatcatgt actggtggta catggccttc ttggaattta acccgatcgc aaggagacgt
     6601 tgccatgtat ttccactcca tcgttccttc cacacctgat cagtctgatt tgccgccaag
     6661 tcagcaagga ctgtcatgct aatcaaagac agtccggagc cggaccttcc tgctactgct
     6721 gcgagttccc ccg
//
LOCUS       DATIPF010000367         6471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_514378, whole genome shotgun sequence.
ACCESSION   DATIPF010000367 DATIPF010000000
VERSION     DATIPF010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6471
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514378"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..1610
                     /locus_tag="VLL06_09215"
     CDS             339..1610
                     /locus_tag="VLL06_09215"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HSS31182.1"
                     /translation="MTLTKGVSNLGYNHVVQGGGGSGVGYVWTVSIGALPPGVTLISG
                     TPNATLRGIPTVTGTFNFTIRVQDSIGQSSTQDLVLQVTDAMVRAVAGTGNAGFSGDN
                     DFATSAELNTPAGVAVDSAGNILISDQLNHRVRRIDGQTKLITTVVGTGVPGFNGDPN
                     ALLTEFNSPVGVGLDASGNWLLIDSGNHLLRRVRVDVQPMLVSTLAGTGIGGFKDGPV
                     ATAQLNGPAGMAVEASGTVLIADSVNNRVRRVNLQTGQVTTVAGTGTGGFNGDNILAS
                     SAQLNLPTGVAVDFSGNIFIADTLNQRIRRIDGLTNEITTVAGTGAFGFNGDNPNATL
                     AQLSFPLGIAVDTAGNVLIADSLSHRVRLVNMQSGEIKTIIGTGAPSYNGDDQLATTA
                     QINAPMGLTVSAIGEVFFTEASGMRVRRIGQ"
     gene            2007..3323
                     /locus_tag="VLL06_09220"
     CDS             2007..3323
                     /locus_tag="VLL06_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31183.1"
                     /translation="MTRNDLLLRLPRERLTLLRELGGLADRGGVSLYLVGGVVRDLLL
                     KRENWDLDLTVEGDGIAFARAVSERYRAGLAIFERFATSRLVFPEGLKLDIATTRRES
                     YAQPAALPDVEFASLKEDLYRRDFTINAMAIQLNTAQFGRLYDPYGGRQDLKAKTLRV
                     LHTCSFRDDPTRIFRAIRFEQRFGFRLERTTMRLLTQAASTNLIQQLSGPRLQNEIVL
                     LLAERDPVRAIVQLAQLKLLRFLHRRLCYTRNVRPVVTAVPKALVWCARRFPESVIDR
                     PTVYLMALSSQSSQAVVATMIRRLALSREQARNLSVGGNRIDRVRKQLIDRETVRPSQ
                     VYRLLADFSNEALVLLLAKQVAIQHKVRLSLLKRRLMAYMKNRTFKTVLTGGDLQTMG
                     LEPGPQYKTILEGLLDARIDGMITTEAEERAFVQKRIGLQRRHAVS"
     gene            complement(3320..3991)
                     /locus_tag="VLL06_09225"
     CDS             complement(3320..3991)
                     /locus_tag="VLL06_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31184.1"
                     /translation="MTHTTPPKALSHDEKKAADAAFAGRPFNEAWSASARAVYDGIVK
                     ALPHTDIALPTPSNVEEPVEAPSMQSEPLQSQEPLAQDQATGQELVEGTTMPPAIRDR
                     EGAIQAGALIDVTPTALELGITFPVTVTRPLWEAGIVTSQPLPEENQTGRLRDILMAF
                     RLRLASLTTVSPLLDFPVLLTLPPSRVPQPVPLFALIQPDPMHQANVTLLLPQEVSLS
                     ITSLN"
     gene            4303..4944
                     /locus_tag="VLL06_09230"
     CDS             4303..4944
                     /locus_tag="VLL06_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HSS31185.1"
                     /translation="MARIDFYRVLGVSRDASDDAIKKAYRRLVFQHHPDLNPDSAHAE
                     PKIREINAAYEVVGNPDKRRTYDRLNWGDVTPGDEPADPAVILDEMEQKLFDEGRKEV
                     FAVLMKDVKRIKAELAMIRERTVADQGYDTFKDEIIRERASEVMEELTTVEMEGRRQR
                     LVEVATEILLSQGVVKRGDEGGVRSLCGGLDEVFRKGRLKGFAAALELFYERR"
     gene            complement(4971..6173)
                     /locus_tag="VLL06_09235"
     CDS             complement(4971..6173)
                     /locus_tag="VLL06_09235"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSS31186.1"
                     /translation="MTIRSVRIIDGTGQTTERATVIIHGSTIAAIGSDHDLSPPRGTC
                     KIDGRGLTLLPGLIDCHVHLCLGAEPDVVDAIAKEPPALTLLKSSQAARRTLEAGFTT
                     VRDVGSRDHSIFTLQRAIDEGLVPGPRIVAAGLVICMIGGHARFIGQEVEGAEQVRSV
                     VRAQIAAGAAVIKVIASGGVLTPGTSPDQAQMTVDELCAAVDESQRAGRKVAAHAHGS
                     SGMKNAIQAGVHSIEHATLMDGEAATMMKQQGVFMVPTLSALATTAACRLGCGIPESA
                     LDKAKSMTKRHQVSFKNALRDGIQIAMGTDAGTPFNFHGENAQELERMVAFGMSPMQA
                     ILASTSAAARLIGIQDKVGTIEKGKLADLLLVEGNPLRRIDLLRDRNRIIGVMQAGKL
                     VAGPLSKT"
     gene            complement(6179..>6471)
                     /locus_tag="VLL06_09240"
     CDS             complement(6179..>6471)
                     /locus_tag="VLL06_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HSS31187.1"
                     /translation="AEHRLPMLTCVTVPSHIPEAEIRTNLLSTYGIEIGGGLGPLKGK
                     VWRIGLMGQSSTEAHVLTLLNALEELFIRSGWLSTPGIALQAASRVYSRTAS"
BASE COUNT         1390 a         1642 c         1953 g         1486 t
ORIGIN      
        1 ttatgttcta tgagtaaaca taaaggtagc gtagggaagg atgcaggttc tggttcagca
       61 agccgtgaga gatggtggct acacgtggca actatcacga ttgcatctac gagttagata
      121 tcaacccatc aaggagaaca atgagaaaga tgagcaggaa tgatgcgatc tcacatgaaa
      181 gcaccaccac ctctatgaca ggaaaacgga tcagttggct aggagtagtg gcgagcagca
      241 tgctcggtat ggtgctcgtg gcaatgtctg cctgtggaga cggaaatgga agtggggcag
      301 tatcttcacg agcgtcttct gcgccgcaga tcgcgacgat gacattgaca aaaggggttt
      361 ccaatcttgg ctataaccat gtggtgcaag gaggtggcgg tagcggagtt ggctatgttt
      421 ggacggtaag catcggtgcc ctcccacccg gtgttacact gatcagcgga actccgaatg
      481 ccacactgcg gggtatccct accgtaactg gtaccttcaa tttcacgatc cgtgtccagg
      541 attctatcgg gcagagcagc acacaagact tggtccttca ggtaactgat gccatggttc
      601 gcgcggtggc gggaaccgga aatgctggtt tcagtgggga caatgatttc gcgacgtcgg
      661 cggagctcaa tacaccagca ggcgttgcgg ttgatagtgc gggaaatatc ttgatctctg
      721 atcaactgaa tcatcgcgtt cgtcggattg atgggcagac gaagttgatt acgacggttg
      781 tcgggacagg cgttccaggt tttaacggag accccaacgc tctcttaact gagttcaact
      841 caccagtggg tgttgggctg gatgcctcgg gaaactggct actgatagac tctgggaatc
      901 atctgctccg gcgagtacga gtagacgtgc agcccatgct cgtcagtacg ttagctggaa
      961 caggtatcgg tgggtttaaa gatggtccag tggctacggc tcaattgaat gggcccgctg
     1021 gtatggcagt agaggcctca ggaactgtgt tgatagccga tagtgtcaat aatcgtgtga
     1081 ggcgcgttaa tctacaaacc ggacaggtca cgactgtggc tgggactggt acaggtggat
     1141 tcaatgggga taacattctg gccagttccg ctcagttgaa cctccccaca ggtgtcgcgg
     1201 tcgatttctc gggaaacatt ttcatcgcag atactttgaa ccaacgtatt cgccgtatcg
     1261 atggtctcac gaatgagatt accacagtgg ctggcactgg agccttcgga ttcaacgggg
     1321 ataatcctaa tgcaacgttg gctcaattga gtttcccgct tgggatagca gtcgatacgg
     1381 ctgggaacgt cctcattgca gattcgctca gtcatcgcgt gagattggtt aatatgcagt
     1441 cgggagagat taaaaccatc atcggaaccg gtgctccgag ctacaacggt gacgatcaac
     1501 tcgctacaac ggctcaaatc aatgctccta tgggtctcac cgtgagtgcc atcggcgaag
     1561 tattctttac tgaagccagc ggcatgcggg ttcgacgcat tggccaatag ccgtggcctc
     1621 ttcttcggtt tgtcatctat atccgtgagg ctagtattgg tttcaaccct tctggttttt
     1681 ccttccgtgc agatcctcta atggattcca tattccgtta gtcttttctc cagcccctcg
     1741 tcgcactcat cagacgaggg gccggcagga tggttcgttt atgacagccc attcaaagct
     1801 tcctatggct gtttttcaat agcctccttt tatcgggctg aacctccgcg ttggttctgg
     1861 aagcagaagt ggcaccgaca atccaaggct gtgtggggat aataggggcg ctcgggatcg
     1921 catcctaagc gccttatctt gttgaggagg aacactgttc ctcctcaaca aggaggttgc
     1981 tacaatggcg tgccatgaat catcggatga cccgaaacga tctgttgctc cgtctccctc
     2041 gggagcgtct tactctgctt cgtgagcttg gcggtctcgc ggataggggt ggggtgtcct
     2101 tataccttgt cggcggagtc gtccgcgatc tcttgctgaa gcgggagaac tgggatctcg
     2161 atcttacggt tgaaggggac ggaatagcct ttgcgcgtgc ggtttccgaa cgatatcgag
     2221 ctgggctggc aatattcgaa cggtttgcga cgtcacggct tgtctttcca gaagggctca
     2281 agctagatat cgcgaccacg cgaagagagt cctacgcaca gcctgccgcg ctaccggatg
     2341 tcgagtttgc ctcattgaag gaagatctct atcgccggga tttcacaatc aatgccatgg
     2401 cgattcaact aaacaccgcg cagttcggac ggctctatga tccctatggt gggcggcagg
     2461 atctgaaggc gaagacactc cgtgtcctgc acacgtgcag tttccgagac gatcctacgc
     2521 gcatcttcag agcgattcgg ttcgaacaac gattcggttt tcgtcttgag cgcaccacca
     2581 tgcgtcttct cacacaagcg gcctccacga atctcatcca acaactctcc ggtcctcgat
     2641 tgcagaacga aattgtattg ctgctcgctg agcgggatcc ggtgcgcgcg atcgttcagc
     2701 tggcacagtt gaagttgctt cggttcctcc atcgccgtct gtgctacacg aggaatgtca
     2761 ggccagtggt cacagccgtt cccaaggcgc ttgtctggtg cgcccgtcgg ttcccggaat
     2821 ctgtaatcga caggcccacc gtctatctca tggcgttgtc gagtcaatcc agccaggctg
     2881 tggtggctac gatgatccgc cggctcgcgc tttcacgaga acaggccagg aacctgagtg
     2941 ttggaggaaa ccggatcgat cgtgtacgga agcaactcat cgatagagag acagtgcggc
     3001 catctcaggt gtatcgcctt ttggcagatt tttctaacga ggcgctcgtc ctacttctgg
     3061 cgaagcaggt tgcgatacag cacaaggtgc ggctgagtct gttgaaacgg cgtctcatgg
     3121 cgtatatgaa gaacaggaca ttcaagacag tactgacggg gggggatctt cagactatgg
     3181 gattggaacc aggtcctcag tataagacta tcttggaagg attattggac gcgcgcatcg
     3241 atgggatgat tacgacggag gcggaggaac gtgcctttgt tcagaagcgg atcggtcttc
     3301 aaaggcgcca cgcggtgagc tagttcagcg aggtgatgga caaggacact tcttgtggca
     3361 gcaagagcgt gacgtttgcc tggtgcatgg gatccggttg aatgagggca aacaatggta
     3421 ccggctgagg caccctgctc ggcggcaggg tcagcaggac cggaaagtcg agtaagggag
     3481 acaccgttgt caaactggcc aggcgcaaac ggaaggccat gagaatatca cgcagccggc
     3541 cagtttggtt ttcttctggg agtggttggc tcgtgacaat tcctgcttcc caaagtggtc
     3601 gggtaactgt gacagggaat gtgataccaa gttccagggc ggtcggcgtc acatcaatta
     3661 aggccccggc ctgaatcgcc ccttcccggt cgcgaatcgc cggaggcatc gtagtgcctt
     3721 ccaccaattc ttgcccggtg gcttggtctt gtgcgagagg ttcttgcgac tggagaggtt
     3781 cgctctgcat agacggagcc tccacgggtt cttcgacatt ggaaggagtg gggagtgcga
     3841 tgtcggtgtg aggtaaggcc ttcacaatac catcatagac tgctcgagct gaagcagacc
     3901 atgcctcatt gaatggacgg cctgcgaagg ctgcgtcggc ggctttcttc tcatcgtggc
     3961 tgagagcttt gggtggtgtt gtgtgtgtca tgctatgaac ataagccgct ttggtgggat
     4021 gtcaaggggg aatgtgtttg acgttgattc caaagctgtt attcaggcat cggtttcccc
     4081 ttgtgatata cagaaccagc cattggtaat gcgtcacacc atcgcgccac gagcctgccg
     4141 ctatagtttg ggtctgcatc aggtgtgtgg ttgtagagag aaggcggacg ttccctctca
     4201 tatcacgccg cgtgcctcag tggttgaagt cgcaccaggc taccgagctt gcgcatgcgt
     4261 gtcagggtgc agggctgaaa ggtgcatggt atagtcgccg cgatggcacg aatcgacttc
     4321 tatcgggtgc tgggagtctc gcgcgacgcg tccgacgacg cgatcaagaa ggcctatcga
     4381 aggctggtct ttcagcacca tcctgatctc aaccccgata gcgctcacgc cgaacccaag
     4441 atccgtgaga tcaatgcggc ctacgaagtt gtcggcaatc cggataagcg gcggacctat
     4501 gaccggctca attggggaga tgtaacacca ggggatgaac cggctgatcc agccgtcatc
     4561 ctcgacgaga tggagcagaa gctgttcgac gaaggtcgga aggaagtgtt cgcggtattg
     4621 atgaaagatg tgaaacggat caaagccgag cttgccatga ttcgtgagcg gaccgtggcg
     4681 gatcaagggt atgacacgtt caaggatgaa atcattcggg agcgggcgtc ggaggtcatg
     4741 gaggagctta ccacagttga gatggagggg cgcagacagc ggctcgtgga agtggcgacg
     4801 gagatactcc tgtcccaagg tgtggtcaaa cgaggggatg agggaggcgt cagatcgcta
     4861 tgtggagggc ttgacgaggt gttccgcaaa ggacgcctga agggtttcgc agccgcgctg
     4921 gaactattct acgaacggcg ttaaacgtga aatgttgtat gtgcaacgtt tcacgtcttt
     4981 gagaggggtc ctgcaaccaa cttgcccgcc tgcatcactc cgataatcct attgcggtcg
     5041 cggagcaggt caatgcgacg aagcggattc ccttcgacca acaggagatc agcgagtttc
     5101 cctttctcga tggttcccac cttgtcttga atcccaatta aacgtgcggc agcggaggtc
     5161 gaggcgagaa tcgcctgcat cgggctcatc ccgaatgcga ccatccgttc gagttcctgg
     5221 gcattctcgc cgtgaaaatt aaagggcgtg cccgcatcgg tgcccattgc aatctggatt
     5281 ccgtcacgca gtgcgttctt aaagctcact tgatggcgtt tcgtcatgga cttggctttg
     5341 tccagggcgc tttcgggaat gccgcatccg agtcggcagg cggcagtggt ggctaaggcc
     5401 gagagtgtcg gcaccatgaa gacaccctgc tgtttcatca tggtcgcggc ttctccgtcc
     5461 atgagtgtgg catgctcgat cgaatggacg ccggcctgaa tcgcattctt cattcccgag
     5521 gatccatggg catgggcagc gactttccgc ccagctcgct gcgattcatc tacggcggca
     5581 cagagctcat ccacggtcat ttgcgcctgg tccggcgagg tgccgggtgt cagaacgccg
     5641 ccagatgcaa tgactttaat gacggcggct ccggcggcga tttgtgcgcg cacgacggaa
     5701 cggacttgct cggccccttc cacttcctga ccgataaatc tggcgtggcc gccgatcatg
     5761 cagatcacca ggcctgctgc gacaatgcga gggccgggaa cgagtccttc gtcgatagcc
     5821 cgttgcaatg tgaagatcga gtggtctcgc gaccccacgt cgcgcacggt ggtgaagccg
     5881 gcctccaagg tccgacgcgc agcctggctt gatttgagca acgtgagagc gggcggttct
     5941 ttcgcgatcg cgtcgacgac atccggttcg gctccgaggc agagatgcac gtgacagtcg
     6001 atcaagcccg gtaatagtgt cagccctcgg ccatcgatct tgcaggttcc acgtgggggg
     6061 gagaggtcgt gatccgatcc gatggcggca atcgtgctgc cgtgaatgat caccgtggcc
     6121 cgttcggtcg tctgaccggt cccgtcgatg atccggactg agcgaatggt cacggacttc
     6181 atgatgccgt acggctataa actcgcgagg cagcctggag tgctattcca ggagtggaaa
     6241 gccagccgct gcggataaac agttcttcaa gcgcattgag taaggtcaag acgtgagcct
     6301 cggttgatga ttgacccatc aagccgatgc gccatacctt tcccttgagt ggaccgagcc
     6361 cgccaccgat ttcaatgcca taggtcgaga gcagattcgt ccgtatctcg gcctcgggaa
     6421 tatgagaggg gaccgtgaca caggtcaaca tcgggagtct gtgttcggcc g
//
LOCUS       DATIPF010000368         5815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_866723, whole genome shotgun sequence.
ACCESSION   DATIPF010000368 DATIPF010000000
VERSION     DATIPF010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866723"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VLL06_09245"
     CDS             complement(<1..336)
                     /locus_tag="VLL06_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSS31188.1"
                     /translation="MIEAAQGNAPSTGSSVKSAKRVGLRATGLVKSFRSRKVVKGVSI
                     EVHAGEIVGLLGPNGAGKTTIFDMIVGLCQPDEGAISLGEEVITDLAMYRRARKGIGY
                     LPQESSVFRR"
     gene            complement(333..914)
                     /locus_tag="VLL06_09250"
     CDS             complement(333..914)
                     /locus_tag="VLL06_09250"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HSS31189.1"
                     /translation="MWIWLLLLNLVPFGMASLGESADAALPKGGVDQAVSTTITAKKM
                     TVKNQDSQAVFEGTVILTRGSLVVYSDHMVVTFRAQDPAAIDNQKGHEAVKGAAPVKE
                     VGMMPAVSNRSVNKIEATGRVKIEKDSGSATCEKAIYYHDGDKIVLTGDPVAWDKGTR
                     VSGKQITMFLAEDRSVVEGGSHVRIEPDGGAGK"
     gene            complement(889..1434)
                     /gene="lptC"
                     /locus_tag="VLL06_09255"
     CDS             complement(889..1434)
                     /gene="lptC"
                     /locus_tag="VLL06_09255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HSS31190.1"
                     /translation="MLALSMVLACFLAYLLMTNSTTIPTPTAAAPGSISTADATISQF
                     TFTQTKGDTVLWQVQAQEARLFEGDKQAMLQAVAVTLFGQRGKELTVTGDEGTLNTET
                     KNFVLANRSEPLVIHTESGYVIYTNHLAWTDQTREIRTQDPVRIVGHGLEVTRRGLLG
                     HLDREEFEVLEDVHVDLAPAS"
     gene            complement(1651..2073)
                     /gene="rplQ"
                     /locus_tag="VLL06_09260"
     CDS             complement(1651..2073)
                     /gene="rplQ"
                     /locus_tag="VLL06_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HSS31191.1"
                     /translation="MRHRKKGRQLGRQTKHRWALFRSLVTSLLEHDRIETTEAKAKEI
                     RGFTDRMITLGKEGTLPARRQALAFIRSKDVVSKLFSDVAVRFKDRPGGYTRTVKTRR
                     RIGDAAKLVAIELVTRPDTTPAKKQASTVSTPASPAAD"
     gene            complement(2092..3093)
                     /locus_tag="VLL06_09265"
     CDS             complement(2092..3093)
                     /locus_tag="VLL06_09265"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HSS31192.1"
                     /translation="MIKAMKDFQIPARVEVDKDTQSKTFGRFTTEAYERGFGTTVGNA
                     LRRVLLSSLTGAAVTTVKIEGVLHEFSTIPGITEDVTSIILNVKGLRLALHTDKPKTI
                     RLKKKGPGEAKGSDIIHDADVTILTPNLHIATLDKDATFDMEMTVKHGRGYVPAERNK
                     EEGLPIGVIAIDSVFSPIKRVNFHVENARVGRMTDYDKLTLEVWADGTISPRDAVSTA
                     AGILRDHIDIFINPDERVEGRADLGGDEAQREVNKHLFRSVNELELSVRAANCLKNAN
                     IKTIADLVQKTEGEMLRTKNFGKKSLNEIKEILTEMGLGLGVKLDTVQQNSGSLKSE"
     gene            complement(3181..3807)
                     /gene="rpsD"
                     /locus_tag="VLL06_09270"
     CDS             complement(3181..3807)
                     /gene="rpsD"
                     /locus_tag="VLL06_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HSS31193.1"
                     /translation="MAKYRGPVCRLCRREGEKLFLKGSRCMTEKCAIERRSYPPGQHG
                     QKRPRNSEYSTQLREKQKLRRIYGLMECQFRGVFERAERQSGITGEVLLRLLECRLDN
                     VAYRLGFGASRKEARQLVSHGHLTMNGRKINVPGAQVKAGDIISVRERSRTLVSIQAA
                     LESVDGRGIPEWLELDKTAFKGMIRALPSKDQISLPVNEQMVVELYSR"
     gene            complement(3889..4272)
                     /gene="rpsK"
                     /locus_tag="VLL06_09275"
     CDS             complement(3889..4272)
                     /gene="rpsK"
                     /locus_tag="VLL06_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HSS31194.1"
                     /translation="MSIKKKKKKERRIVQSGVAHVQASFNNTIVTITDMSGNTIVWAS
                     SGNQGFKGSRKSTPFAAQRAGEAAARKAMESGMRQVDVYVNGPGSGRESAIRSLQAAG
                     LRIHMIRDVTPIPHNGCRPPKRRRV"
     gene            complement(4304..4690)
                     /gene="rpsM"
                     /locus_tag="VLL06_09280"
     CDS             complement(4304..4690)
                     /gene="rpsM"
                     /locus_tag="VLL06_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HSS31195.1"
                     /translation="MARISGVDLPREKRTDIGLTYVYGIGRASALAILGKAGIDGSIR
                     VKDLSEEHIVKIREIIQEGYQVEGDLRKTFSMNIKRLIDSGTYRGLRHRKGLPVRGQR
                     TKTNARTRKGRRSGVGSKPKPPVRSA"
     gene            complement(4823..4936)
                     /gene="rpmJ"
                     /locus_tag="VLL06_09285"
     CDS             complement(4823..4936)
                     /gene="rpmJ"
                     /locus_tag="VLL06_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HSS31196.1"
                     /translation="MKVRSSVKPICAKCKVVRRKGVVRILCENPRHKQRQG"
     gene            complement(4952..5170)
                     /gene="infA"
                     /locus_tag="VLL06_09290"
     CDS             complement(4952..5170)
                     /gene="infA"
                     /locus_tag="VLL06_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HSS31197.1"
                     /translation="MPKEDVIEIQGTVNETLPNAMFRVSLDNGHKILAHISGKMRMHF
                     IRILPGDKVTVELSPYDLTRGRITYRFK"
     gene            complement(5217..>5815)
                     /gene="map"
                     /locus_tag="VLL06_09295"
     CDS             complement(5217..>5815)
                     /gene="map"
                     /locus_tag="VLL06_09295"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HSS31198.1"
                     /translation="GARPAFKGYRSYPKTLCASVNDQVVHGIPSKRVLKEGDIIGLDL
                     GAIVGGFYGDSAVTVSVGQTTERIARLVQVTEEAMYLGIEQAVPGHRLSDISHAVQRH
                     VEAAGYSVVTEFVGHGIGRQLHEEPQVPNYGKPGQGPRLQAGMVLAIEPMVNMGGAAV
                     KILEDRWTAVTVDGSLSAHFEHTIAIEPSGPPRILSQL"
BASE COUNT         1233 a         1848 c         1324 g         1410 t
ORIGIN      
        1 gcgtcgaaac accgatgatt cttgcggaag atacccaatg ccttttctcg cgcgtctgta
       61 catggcgaga tccgtgatca cctcttctcc gagcgagatc gccccctcat caggctggca
      121 cagtccgacg atcatatcga aaatggtggt cttcccagcc ccattgggtc ccagcaaacc
      181 cacgatctcc ccggcatgga cttcgatcga aacgcccttg acgaccttac gcgagcgaaa
      241 acttttcacc aagcctgtgg cgcgtaagcc gacacgtttc gcactcttca ctgaagagcc
      301 tgttgacggc gcattaccct gagccgcctc gatcacttgc ccgcccctcc gtccggctcg
      361 atccgcacat gcgagcctcc ttctacgaca ctccgatctt ctgccaaaaa catcgtgatt
      421 tgtttgccgc tgacccttgt tcccttatcc caagctaccg ggtcacctgt caaaacaatc
      481 ttatctccat catggtagta gatcgctttc tcacaggtcg cactgccgga atccttctcg
      541 attttcactc gtcccgtcgc ctcaatcttg ttgaccgaac gattcgacac cgccggcatc
      601 atcccaacct ctttcaccgg tgcagccccc ttgacggcct catgcccttt ctggttatcg
      661 atcgcagctg gatcttgtgc gcgaaacgtc accaccatat gatcggaata gaccaccaat
      721 gacccacgcg tcagaatcac cgttccttca aacaccgctt ggctatcctg attctttacg
      781 gtcatctttt tggcggtaat cgtcgtgctg accgcttgat caaccccacc tttgggaagg
      841 gctgcgtcag ctgattcgcc caacgaagcc atcccaaacg gaaccaaatt aagaagcagg
      901 agccaaatcc acatggacgt cctcaagtac ctcaaattct tcgcggtcca aatgtcccaa
      961 cagcccacgt cgcgtcacct ctaacccatg cccaacgata cgaacgggat cctgcgtgcg
     1021 tatctctctc gtctgatcag tccaagccag atggttagta tagatcacat atccactctc
     1081 agtgtggatc acgagtggct ctgatcggtt tgcgagcaca aagttctttg tctctgtatt
     1141 caatgtccct tcatctccag tcaccgtcaa ttccttcccc cgctggccga atagtgtcac
     1201 ggcaaccgct tgaagcatcg cctgcttgtc cccctcaaag agccgagctt cttgtgcctg
     1261 tacttgccat agcaccgtat cgcccttcgt ttgggtgaac gtaaactgag agatcgtggc
     1321 atctgcggta cttatggatc caggtgccgc cgccgtcggg gttggaatcg ttgtcgaatt
     1381 ggtcatcagc aggtacgcga ggaaacaggc caacaccata ctgagggcta acaaacctcc
     1441 ccttacccag cgttgccaca cggtgttccc acttcctttc acgcagcatc aatatgactg
     1501 gcacgatagt agcatgcggg ttaaatcaag taaacgtatg ccagaaccgc ggcataatcg
     1561 actcggtgga ggtggagtga acgaaagaga gcaatcgtcg aagcgatatc acggcgtcac
     1621 accttcgact agatcccgtc aacaggagag ctagtcggca gcgggactcg caggagtcga
     1681 cacggtagat gcttgtttct ttgcaggcgt cgtatccggc cgcgtgacga gttcaattgc
     1741 gacaagcttc gcggcatcac cgatccgccg tctcgtcttc accgttctcg tatagccgcc
     1801 aggacggtct ttaaaccgaa ccgctacgtc gctgaataac tttgagacga cgtccttact
     1861 tcggataaac gcgagggctt gccgccgagc cggcaatgtc ccctccttgc cgagcgtaat
     1921 cattcgatcc gtgaatcctc gaatttcctt cgccttggcc tcagttgtct cgatgcgatc
     1981 atgttctagc agcgaggtca cgagactccg gaacagggcc caccgatgtt tggtctgtcg
     2041 tccgagctgt ctgccttttt ttctatgacg cacgggtttc ctcttgtttc gttactcgct
     2101 cttcaaacta ccgctgttct gttgtacggt atccagtttc acaccgagac ccaggcccat
     2161 ttccgtcagg atttctttaa tctcgttgag cgatttcttg ccgaaattct tcgtcctcaa
     2221 catttcccct tcggtcttct gaactaagtc ggcgatggtc ttgatattcg cattcttcaa
     2281 acagtttgca gcacgaacgg acagctctaa ctcgttcacg ctacggaaca gatgcttatt
     2341 gacctcacgt tgggcctcat cgcccccaag gtcagcacgc ccttcgactc gctcatcagg
     2401 attgatgaaa atgtcaatgt gatcacgtag aatgccggcc gccgtggata ctgcatcgcg
     2461 gggcgagatg gtcccatccg cccagacctc caaggtcaac ttatcataat cggtcatgcg
     2521 accgacgcgg gcattctcca cgtgaaaatt cacccgtttg atgggtgaaa acaccgagtc
     2581 gatcgcaatc acaccaatcg gcagcccctc ttccttgttt cgctcggcgg gcacgtagcc
     2641 tcgcccgtgc ttcacggtca tttccatatc aaatgtggca tccttgtcca atgtcgcaat
     2701 atgaagattc ggggtcagaa tggtcacgtc tgcatcatga ataatgtcag accccttggc
     2761 ttcaccaggt cccttcttct tgagacggat ggtcttcggc ttgtcagtat gtaaggcaag
     2821 ccgcagtcct ttgacattca aaataatgga ggtcacatct tcggtgatac cagggatcgt
     2881 ggagaattcg tgcaggaccc cttcaatctt cactgtggtc actgccgcac cagtcaagga
     2941 cgataacagc acgcgccgta acgcattgcc gaccgtggtc ccaaacccgc gttcataggc
     3001 ctctgtcgta aatcttccga aggtttttga ctgggtatct ttgtcgactt ccacccgtgc
     3061 tgggatctga aagtccttca tcgctttaat catgactccc ccttcatctc atcaagtagc
     3121 cagccactga accgtaccag cttgccgcag aggccccttc atctccgaca tccacatcta
     3181 ttatctggaa tacagctcca ccaccatctg ctcattcacc ggcaacgaaa tctggtcttt
     3241 cgacggcaag gctcgtatca tgcctttaaa agcagtcttg tcgagctcta gccattctgg
     3301 aatgcctcgc ccatcaacag actctaaggc tgcctggatc gagaccaggg tgcgacttcg
     3361 ctcccgaacg ctgatgatat ctccagcttt gacctgcgca ccaggcacgt tgatttttcg
     3421 accattcatg gtcagatgcc catggctcac cagttgtctg gcttcttttc gtgaggcacc
     3481 gaaccctagt ctgtaggcca cattatccaa ccggcattcc agcagccgca agaggacttc
     3541 gcccgtaatc ccagactggc gttccgcacg ctcaaacacg ccacggaact ggcattccat
     3601 caatccataa attctccgta gcttctgctt ttctctgagc tgtgtactgt actctgaatt
     3661 cctcggacgc ttttgcccat gctgtccggg aggatagctc cgccgctcaa tggcgcattt
     3721 ctccgtcata cagcgcgagc ctttcagaaa caatttctca ccctctcgcc gacacaaccg
     3781 acagacggga ccgcgatact ttgccactgc tctacctccg gttaccggac atcaccacag
     3841 aggcgtgtcc aacaattctc gtgtattcga ctgtctcaac atctgccatt agacacgccg
     3901 ccgtttaggt ggacggcacc cgttgtgcgg aatcggggtg acatcacgaa tcatatggat
     3961 ccgcaatcca gcagcctgaa gagatcgaat cgccgattca cgaccggacc caggcccatt
     4021 cacatagaca tccacttggc gcatgccgct ctccatcgcc ttgcgcgcag cagcttcgcc
     4081 ggcccgctga gccgcgaagg gcgtactttt acgagaaccc ttgaagcctt ggttccctga
     4141 gctcgcccac acaatcgtgt taccactcat atcggtgatc gtgacgatcg tgttgttgaa
     4201 ggaggcctgg acatgggcca ccccgctctg cacgatccgg cgctctttct tcttcttttt
     4261 cttgatgctc atacaggctc cctactcaga taacggtcac acactatgcc gaacgcacag
     4321 gcggtttcgg tttgcttccc acacccgagc gacggccttt tctcgtccgc gcattcgtct
     4381 tggtccgctg gccgcggact ggcaaccctt tccgatgacg cagaccgcgg tatgtaccgc
     4441 tatcaatcaa tcgtttgata ttcattgaga aggtcttcct cagatcgcct tcgacctgat
     4501 atccctcctg aataatttct cgaatcttga cgatatgttc ttcgctcaag tccttgacgc
     4561 gaattgatcc atcgattcca gccttgccca ggatggcaag tgcggatgcc cgcccgatcc
     4621 catagacata ggtgaggcct atgtccgtcc gtttttccct cggcagatcc actcccgaaa
     4681 tacgagccat agttcctcct accctcttcc tctacattct cagcggccgc ggctcggtga
     4741 gcctcaactg cgcgcatcga acgagcgccg tctcatcgcg cgcgttctgc gagcatggag
     4801 gccacccagc cgcggccgcc ccctaccctt ggcgctgttt gtggcgcgga ttctcgcaga
     4861 gaattcgcac cacgcccttg cgccggacta ccttacattt tgcacaaatc ggcttgacgg
     4921 atgatcgcac tttcatgtct ctttcacact cctatttgaa ccgataggtg atccgcccgc
     4981 gtgtcagatc gtacggtgac agttccacag tcaccttatc gccgggaaga atacggataa
     5041 agtgcatgcg cattttcccg gaaatatgtg caagaatctt atggccattg tccagcgaca
     5101 cacgaaacat cgcattcggt aaggtttcgt tcaccgtgcc ttgaatttca atcacatctt
     5161 ctttcggcac gtacctctat cctttctccc cgaactggac gacgacaccc ccaactctat
     5221 agctgactca ggatacgcgg aggcccgctc ggctcaatcg caatcgtatg ttcgaaatgc
     5281 gccgagaggc tcccgtctac tgtcacggcc gtccaacgat cctcaagtat cttgacggca
     5341 gcaccaccca tattcaccat cggttcgatc gcgaggacca ttcctgcctg caaccgtggc
     5401 ccctgcccag gcttcccgta attcggcacc tgcggctcct catgtaattg ccggccgatc
     5461 ccatggccga cgaattccgt tacaaccgaa tagccggcag cctccacatg acgttgaacc
     5521 gcatgcgaga tatctgacaa ccgatgaccc ggcaccgctt gctcgattcc gagatacatg
     5581 gcctcttcgg tcacttgcac caaacgggca atccgctcgg tagtctgccc caccgagacc
     5641 gtgaccgccg aatctccgta aaatccacca acgatcgccc ccaggtcaag cccgataata
     5701 tccccttcct tcagcactcg cttggaagga atcccatgga caacctggtc attcaccgag
     5761 gcgcagagcg ttttggggta actccgatac cccttaaacg ccggtctggc accgc
//
LOCUS       DATIPF010000369         3476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1223966, whole genome shotgun sequence.
ACCESSION   DATIPF010000369 DATIPF010000000
VERSION     DATIPF010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223966"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /gene="rpsE"
                     /locus_tag="VLL06_09300"
     CDS             <1..469
                     /gene="rpsE"
                     /locus_tag="VLL06_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HSS31199.1"
                     /translation="VFINRVAKVVKGGKRFNFCALVVVGDGQGWVGIGKGKAAEVPVA
                     ISKAVEQAKKNLVHVALTSGTIAHEVHGLFGAEHVLLKPAQKGAGIIAGGAVRAVVEV
                     AGVHNVIAKTLGRGNPFNTVRATLNGLCQLRNAEDVLKMRRSMVGDVQNKVSA"
     gene            469..678
                     /gene="rpmD"
                     /locus_tag="VLL06_09305"
     CDS             469..678
                     /gene="rpmD"
                     /locus_tag="VLL06_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HSS31200.1"
                     /translation="MPAAKIAKASLKGFVITLRRSPIGTPQKHRRVLSGLGLRKIRQT
                     VTRPNTPQVRGMIGKVGYLLEVQPQ"
     gene            675..1130
                     /gene="rplO"
                     /locus_tag="VLL06_09310"
     CDS             675..1130
                     /gene="rplO"
                     /locus_tag="VLL06_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HSS31201.1"
                     /translation="MNLHELGPAKGSRKRRKRIGRGPGSGHGKTSGKGHKGLLARSGG
                     RSRQAGGFEGGQMSLIRRVPKHGFTNVFRKEYSIVNLKSLGDMVVSGTITPQALVDVG
                     LVKRKSLPIKILGSGDLTKAIVVQAHKFSKSAEAKIRAAGGRVEVIPGV"
     gene            1123..2442
                     /gene="secY"
                     /locus_tag="VLL06_09315"
     CDS             1123..2442
                     /gene="secY"
                     /locus_tag="VLL06_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HSS31202.1"
                     /translation="MFERLLTSFQNILKIPELRTRILFTVGMLVVYRIGAHIPTPGIN
                     GDALSEFLQKQGGALLGFLDIFSGGSLSRLTILALGIMPYISASIILQLLTVVVPHLT
                     KLAKEGERGRKKIIQYTRFGTIGIAVIQGFGIAVGLEQMNNGAFVMSGGWGFRLMTVI
                     TLTAGTGFLMWLGEQITERGIGNGISLIIFAGIVARLPSAVAQTFNLYTIGQLSIILL
                     VALAVLMVVVVAAIVFLESGRRKVPVQYAKRVIGRRVFGGQSTHIPLKINTAGVIPPI
                     FASSIIAFPATIAGFFETPWVKDIGAQLAPGSLLYTLMYVGLILFFCFFYTAVVLNPV
                     DMADNMKKYGGFIPGIRPGTRTSDYIYKVLTRITFAGSIYLAIVCVIPEFLIYKLNVP
                     FYFGGTSLLIVIGVGLDTAQQIESHMLMRNYEGFLGKGMAPLRGRSG"
     gene            2452..3108
                     /locus_tag="VLL06_09320"
     CDS             2452..3108
                     /locus_tag="VLL06_09320"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HSS31203.1"
                     /translation="MRVVFLGAPGVGKGTQADRLAAQYQVAKISTGDLLREAVRNQTT
                     LGLEAKSYMDQGKLVPDAVVIGLVKEKLTDPSCGKGFVLDGFPRTVPQAEALGVVLAS
                     KGIALGRVVNFQVSREDIVRRLSGRRSCPKCQATFHVNFAPPKVDGICDRCGEPLVQR
                     SDDRREAIEMRLKVYDEQTAPLVRYYQERGLLFQLDGSGGVDVVFQHLSQELAPYRVA
                     "
     gene            3105..>3476
                     /locus_tag="VLL06_09325"
     CDS             3105..>3476
                     /locus_tag="VLL06_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HSS31204.1"
                     /translation="MIILKTPDEIALMAQASRVVAETLQILQKAVRPGITTDELDRLA
                     EDEIRSRGARPAFKGYRSYPKTLCASVNEQVVHGIPSKRVLKEGDIIGLDLGAIVGGF
                     YGDSAVTVSVGQTTERVARLVQ"
BASE COUNT          748 a          815 c         1085 g          828 t
ORIGIN      
        1 ggtcttcatc aaccgcgtgg cgaaagtcgt gaagggtgga aagcgtttca acttttgcgc
       61 gctggttgtt gtcggggatg gacaggggtg ggttggtatt ggaaaaggga aggctgccga
      121 agtgccggtt gcaatctcga aggcggttga acaggcgaag aaaaacttag tccatgtcgc
      181 cctcacctct ggaacgattg cgcacgaggt ccatgggttg ttcggagccg agcatgtcct
      241 cctgaagccc gcacagaagg gagcaggaat tattgctgga ggcgctgtcc gggcggtagt
      301 cgaggtcgca ggtgtccata acgtcattgc gaagacgctg ggccgtggaa acccctttaa
      361 tacggtccgc gccacgctca acggtctctg tcaactccgc aacgctgaag acgtgcttaa
      421 aatgcgccgc agtatggtag gcgacgtaca gaataaggtg agtgcctaat gcctgcagcc
      481 aagatcgcca aagccagcct caaggggttc gtgattacct tgcgacgaag tcctattggc
      541 acaccgcaaa aacatcgacg tgtcttgagt ggtctggggt tgaggaagat caggcagacg
      601 gtgactcgcc ccaatactcc acaagtcaga gggatgattg gtaaagttgg gtatttattg
      661 gaagtgcagc cacaatgaac ttacatgagc tcggtccagc caaaggatct aggaaacgac
      721 gtaagcgtat cggccgtggt cccggttctg gtcatgggaa aacgtcgggc aagggtcata
      781 agggtctgtt agccagatcc ggcggtcgga gccgacaggc cggtggattc gaaggcggtc
      841 agatgtccct gatccgtcga gtaccgaagc atggctttac gaatgtattt cgtaaggagt
      901 actcgattgt caatctgaaa agcttgggtg acatggttgt atccggtacc atcacacccc
      961 aggcgctggt cgatgtcggc ttggttaagc gcaagtcgtt gccgatcaag attcttggaa
     1021 gcggagacct cacgaaggca atcgttgtgc aggcccataa attcagtaag tccgcagagg
     1081 caaagattcg agcagctgga gggagagtcg aggtcattcc cggtgtttga gagactcctg
     1141 accagttttc agaatatttt aaaaatccct gagctgcgca ctcgcatcct ctttaccgtg
     1201 gggatgttgg tggtatatcg gatcggggcc catattccta cgcctgggat caatggcgac
     1261 gccctctcgg agtttctgca aaagcaaggc ggcgcactgc tcgggttttt ggacattttt
     1321 tcaggcgggt cgctctcgcg gttgacgatc ttagcgctgg gcattatgcc ctacattagc
     1381 gcttcgatta ttcttcagtt gctgaccgtg gtggttcccc acctcacaaa gctggcaaaa
     1441 gagggcgagc gcggtcgaaa aaaaattatt cagtacaccc ggttcgggac gatcggcatt
     1501 gcggtgattc agggcttcgg gattgccgtc ggtctcgaac aaatgaataa cggtgcattc
     1561 gtgatgagcg gtgggtgggg attccgtctg atgacggtca tcaccttaac ggccggcacc
     1621 ggctttctga tgtggctcgg tgaacagatt accgagcgag gtatcggcaa cggcatttcc
     1681 ctgattattt ttgcggggat cgtcgcgcgc ttgccttccg ctgtggccca gacatttaat
     1741 ttgtacacga tcgggcagct cagcattatc ttgctggtcg ccctcgccgt actgatggtc
     1801 gtggtggtgg cggccattgt ctttctagaa agtggacggc gcaaagtacc ggttcagtat
     1861 gcgaaacggg taatcggaag acgggtcttc ggcgggcaga gcacgcatat tcccctgaag
     1921 attaacacgg ccggggtcat tcccccgatt tttgcgtcgt caattatcgc atttcctgca
     1981 acgattgccg ggtttttcga aacgccatgg gtgaaggaca tcggcgcaca actcgcgccg
     2041 ggatcgctgc tttatacact gatgtacgtg ggccttattc tgtttttctg tttcttttat
     2101 acggcggtgg tcttgaaccc agtggacatg gcagacaata tgaagaagta cgggggattc
     2161 attcccggga ttcgccctgg aacccggact tccgactaca tctataaggt cctgacgaga
     2221 attacgtttg ccggatcgat ttatctggcg atcgtctgtg tgatcccgga gtttttgatc
     2281 tataaactga atgtgccttt ttatttcggg ggtacctcac tgctgattgt catcggtgtc
     2341 ggcctcgata cggcccagca gattgaatcc cacatgttaa tgcgaaatta cgaagggttt
     2401 ctcgggaaag gcatggcacc gcttcgtgga cggagtgggt gaggggcatt catgcgtgtg
     2461 gtatttctcg gcgcaccagg agtgggcaaa gggactcagg ctgaccgtct cgcggcgcag
     2521 tatcaggtgg caaagatttc gaccggtgat cttctccgag aggcggttcg taatcaaacc
     2581 acgttgggtc tcgaagccaa gagctacatg gatcagggga aacttgttcc tgacgccgtg
     2641 gtaatcggcc tggtgaagga gaagttaacc gatcctagct gcggaaaagg atttgttctg
     2701 gatgggtttc ccaggactgt tccgcaagcg gaagcattgg gagtggtgtt ggcctccaag
     2761 ggaattgcgc tgggccgggt cgtgaacttt caggtctcgc gagaggatat tgtgaggcgg
     2821 ctgagcggac gccgaagttg cccgaagtgc caagcgacgt tccatgtaaa ctttgcgccg
     2881 cctaaggtgg atgggatttg cgatcgatgc ggagagccgc ttgtgcagcg tagcgatgac
     2941 cgtcgcgagg cgatcgagat gcgcctgaaa gtatatgacg agcaaactgc tccgcttgtg
     3001 cggtattacc aggagcgggg gctgttgttt cagctggatg gttctggggg agtagatgtt
     3061 gtcttccagc atctttcgca ggagctggca ccataccggg tggcatgatc attctcaaga
     3121 cgccggacga gattgcgttg atggctcagg cgtcaagagt cgtagcagag acgttacaga
     3181 tattgcagaa ggccgtccgg cccggaatca cgaccgatga gctggaccgt ttagccgagg
     3241 atgaaattcg gtcccgcggt gccagaccgg cgtttaaggg gtatcggagt taccccaaaa
     3301 cgctctgtgc ctcggtgaat gagcaagttg tccatgggat tccctccaag cgagtgctga
     3361 aggaggggga tattatcggg cttgatctgg gggcgatcgt cggcggattt tacggagatt
     3421 cagcagtcac ggtttcggtg gggcagacta ctgagcgggt tgcccgttta gtgcaa
//
LOCUS       DATIPF010000370         4168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1485536, whole genome shotgun sequence.
ACCESSION   DATIPF010000370 DATIPF010000000
VERSION     DATIPF010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485536"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1226
                     /locus_tag="VLL06_09330"
     CDS             120..1226
                     /locus_tag="VLL06_09330"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HSS31205.1"
                     /translation="MRIGFDANPMVGDMGGVGWHSYHLLRKMLAQHEGPDFVAYARPG
                     AEQPDSVRGWPGVERLQWVNSSRWGMGKRGSSDRLDLYHGTNFKMQAVGRYGGVVTIH
                     DLWLDRHPEYSKKMLGQWPSSFKTRQTALRARKAITVSEFSARELMELYGLKREHIRV
                     IPNGVSEDFVPRRDERAMAELRSRIGLTSGHYVLFIGGADPRKNHQIFLEAAEMVRKK
                     LGSRRLVLVGSPTHPFGSYEESARRRSLTEQVLCPGRMSTNDLQLLYSSTDLFVFPSL
                     YEGFGMPVLEAMACGAPVVTSNSTALAEVAGDAAVLANPQDARALGAAMIRVLEDEPL
                     RAALRVKGFARAKQFSWEQAARQTLAMYQDLCSG"
     gene            1235..2260
                     /gene="rfaQ"
                     /locus_tag="VLL06_09335"
     CDS             1235..2260
                     /gene="rfaQ"
                     /locus_tag="VLL06_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HSS31206.1"
                     /translation="MNNILVIKLRYIGDVLLATPTLRAIKAVRPDVRITMMVNRGTED
                     VLSGNPDVDEILVLDKGSLGAQSRLIAGLRGRQFDTVIDLTDGDRSAFLTWISGAPVR
                     IGFNDEHRWRGRYYTEVVPSVPAVRHRIDRDLEALKPLSIQAGSKDPQLWLTPEEKTS
                     ADQLLHQLGIQASKSFVILQPGARYWFKAWPPERFADLADRLTSQHGCQVLIGGSDQD
                     LDLARQIQQKATCHAIIMAGRTTIKQFAAIAKQSSLFVGSDSGAMHMASAVGTPVVAL
                     FGPSSPCEWGPRGGPVEVLYKEIDCRSCFHPTCTRGEENCMKLISVEEVMAAAGHMMG
                     QPRMAVL"
     gene            2281..3357
                     /locus_tag="VLL06_09340"
     CDS             2281..3357
                     /locus_tag="VLL06_09340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSS31207.1"
                     /translation="MTRSGSSELSNPNMERVACDLCQSDQSDVIARQRDLLLAVTNDE
                     FTIVRCRQCGLIYLNPRPSKDLLGSYYPTVYYPPVQVKTRPQFQQQAKRLSARIKRWV
                     LEDYYGYPSDGSAGWRRYVRRVLLWPDKTLRELKGRHPLPWRGEGKVLDVGCGAGGNL
                     KTLQDQGWDPYGIEISDVAAAHARELVAGNIHTGTLESAPFPPQSFDLVFMSHSLEHL
                     PSPVDALRRVHRLLKDDGLLAVSVPNVNSLEFKLFGRWWFPLDPPRHFYHFEKSSLSE
                     IMAQAGFQLYRFRTGVSAIYLMASLDRVWKHRFQRDVPLRKLIDRLVARPMSLIAGHL
                     GYGTEMTIYALKVQRGADAPSRTV"
     gene            3505..4092
                     /locus_tag="VLL06_09345"
     CDS             3505..4092
                     /locus_tag="VLL06_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HSS31208.1"
                     /translation="MDLQGQRDLLLLNELDRNGGATQRTLAIKLGVALGLTNLYLKRL
                     ARKGYIKITTIPRNRIRYLLTPQGFTEKSRLTYLYMQYSLSYYRDMRVRFTEMMSTFD
                     ATHHQRVVIYGTTELAELAYLSLREMNIDCVGFIDGSSRESFLSCPVSSPEGIVGWQF
                     DRVLITDLEHAAVCEERLVQSGVPREKVLRLGLPE"
BASE COUNT          896 a         1013 c         1296 g          963 t
ORIGIN      
        1 gatggagtcc gacgagagca gatgggctgc aatgggaagc tgcgtgcacg ggaacgattt
       61 agtctcgatg cggctgtcag gcacatggaa cagttctatg gggaggtatt gaagcaagga
      121 tgagaattgg gttcgatgcc aatccgatgg tgggcgatat gggcggtgtg ggctggcata
      181 gctatcatct gctccgtaag atgttggcac agcacgaggg accggatttt gtcgcctatg
      241 cgaggccggg cgctgaacag cctgactctg tgagggggtg gccgggggtt gagcggcttc
      301 agtgggtgaa ctcgagccga tggggaatgg ggaagcgggg ttcatccgat cggttagatc
      361 tgtatcatgg aaccaatttt aaaatgcagg cagtcgggcg ctatggcggg gtcgtcacga
      421 ttcacgatct ctggctggat cgtcaccccg agtattcgaa gaagatgttg ggacaatggc
      481 catcgtcgtt caagacgagg caaacggcat tgcgagccag aaaagccatc acggtgtcgg
      541 agttttccgc gagagaattg atggaactct atgggttgaa gcgcgagcat atcagggtga
      601 ttcccaatgg agtatcagag gattttgtgc cacgccggga tgagcgggcg atggcggagt
      661 tgcgaagtcg gatcggtttg acgtccgggc actatgtctt gtttattgga ggcgcagatc
      721 cgcgtaagaa tcaccagata tttctcgaag cggccgaaat ggtacggaag aagctcgggt
      781 cgcgcaggtt ggtcttggtc ggctcgccca cccatccgtt cgggagctat gaggagtcgg
      841 ccagaaggcg cagtctcacg gaacaggtac tctgccctgg acggatgtct acgaatgatc
      901 tgcagttgtt gtattcttct accgatctgt ttgttttccc ctcgctgtac gaagggtttg
      961 ggatgcctgt gttggaagcg atggcctgtg gggcgccggt ggtgacgtcc aattcgaccg
     1021 cgctggctga agtcgcgggt gatgcggcgg tcttggcaaa tcctcaagac gcgcgggcgc
     1081 ttggagcggc gatgattcga gtgctcgagg atgagccgct tcgagcggcg ttgagggtca
     1141 aagggtttgc tcgtgccaag cagttttcct gggaacaggc cgcgcggcaa acgctggcaa
     1201 tgtatcaaga tctgtgtagt ggttgagcga gctcatgaac aacattttgg tcatcaagct
     1261 ccgctacatt ggggacgtgt tgttggcaac accaaccctc cgtgccataa aagcggtacg
     1321 gccggacgtc cggatcacga tgatggtgaa tcgagggacg gaagacgtcc tgtccgggaa
     1381 tccggacgtg gacgaaatct tggtcctcga caaggggtct cttggagcac agtcacggct
     1441 tatcgcgggg ctgcgtggtc ggcaattcga tacggtgatc gatttgacgg atggagaccg
     1501 gtctgcgttt ctgacatgga tcagcggtgc gccggttcgg attggattta acgacgaaca
     1561 tcgttggcga ggacggtact acacagaggt ggtcccgtct gtgcctgctg tgcggcacag
     1621 gattgaccgg gacctggaag cgctcaagcc cctgagtatc caagccggtt caaaagatcc
     1681 ccaactgtgg ctgacgccgg aagagaagac cagtgcggat caattgttac atcaactcgg
     1741 tattcaggca tcgaagtcgt ttgtgatcct gcagcccggc gcgcggtact ggtttaaagc
     1801 ctggcctccg gagcggttcg cggacctggc cgatcgtctc acgtctcagc atggttgtca
     1861 ggtgttgatc ggtggcagcg atcaagacct cgatctcgca cgacagattc aacagaaggc
     1921 aacatgtcac gccatcatca tggcaggacg tacgacgatt aaacagttcg cggccatcgc
     1981 caagcagtca tcgctttttg tgggcagcga tagcggcgcg atgcacatgg cctccgcagt
     2041 gggcaccccc gtggtcgccc tgtttggccc atcgagccct tgtgagtggg ggccgcgtgg
     2101 tggtcccgta gaggtgctct ataaagagat cgattgccgc agttgctttc acccgacctg
     2161 tacgagaggc gaagaaaatt gtatgaagtt gatatcggtt gaagaagtga tggcggcagc
     2221 tggtcacatg atggggcagc cccggatggc agtgctgtaa atgggtggga aggatcggtc
     2281 atgactcgat ctggttcgag tgaactgtca aatccgaata tggagagggt ggcgtgtgac
     2341 ctctgtcaat ccgatcaatc cgacgtgatc gctcgacagc gggatctttt gctggcagtc
     2401 accaatgacg aattcacgat tgtccgttgc cgccaatgtg gtttgatcta tctgaatccc
     2461 cgcccctcaa aagacctcct cggttcctac tacccaacgg tctattaccc gccggtgcag
     2521 gtaaagaccc ggcctcaatt tcaacagcag gcgaaacggt tgtcggcacg gatcaagaga
     2581 tgggtgttgg aagactatta tgggtaccca tcggatgggt cggctggatg gcggcgctat
     2641 gtgcggcgcg tgctattgtg gcctgacaag acattgcggg agctgaaagg gcgtcatccc
     2701 ttgccgtgga ggggggaggg gaaagtgctg gatgtcggct gtggggccgg cgggaacttg
     2761 aaaaccttgc aagatcaagg gtgggatccc tatgggatcg agatcagtga cgtcgctgcg
     2821 gcacatgcgc gcgagctcgt ggcgggcaat attcacaccg ggacattgga gtcggccccg
     2881 tttcctcctc agtcctttga tctggtgttc atgagccatt cactcgaaca tctcccgagc
     2941 ccggtggatg cgttgcgtcg agtgcatcga ttactgaagg atgacggact gttggcggtc
     3001 agtgtcccca atgtgaatag cctagaattc aagctgtttg gccgctggtg gtttcccctc
     3061 gatccgccac gacatttcta tcatttcgaa aaatccagcc tatcggagat catggcccag
     3121 gcaggatttc agctgtatcg ttttcgaacg ggtgtcagcg ccatctacct catggcgagc
     3181 ctcgatcggg tctggaaaca ccggtttcag agggatgtgc cgcttcggaa attgatcgac
     3241 cgactcgttg ccagaccgat gtcgctgatc gcaggtcacc tgggctatgg caccgaaatg
     3301 accatctatg ctctcaaagt gcagcgggga gcggatgctc cttcccggac tgtgtagaga
     3361 cttctctcct aataaaatct gcctcaaaac tcctacttac agcaatacgg caaaggccag
     3421 cgctctcaaa gtaagaaatc tgcttgacag gtttgcccat tttgttcata ttctgaacac
     3481 acagaaagtt tatctgtata aatcatggat ttacaaggtc aaagagatct tctactcctc
     3541 aacgagctcg accgaaatgg tggggccaca caacgcacgc ttgcgattaa actcggtgtg
     3601 gcactcggtc tgaccaatct ctatctcaag cgtctggccc gcaagggcta tatcaagatc
     3661 acgaccattc cgcgtaatcg aatcagatat ctcctgacgc cacagggctt caccgaaaaa
     3721 tcacgcttga cctatctcta catgcagtac tccctctcgt actatcgtga catgcgtgtc
     3781 cggttcactg agatgatgtc gacattcgat gccactcatc accagcgggt tgtgatctat
     3841 gggacgaccg agttagctga attggcgtat ctgtcgctac gcgagatgaa tattgactgt
     3901 gtgggattta ttgatggcag ctcgcgtgag tcattcctct cttgtcccgt ttcttcgccg
     3961 gaggggatcg ttgggtggca atttgatcgg gtgttgatta cggatctcga acatgcggca
     4021 gtgtgtgaag aacgattggt acagtcgggt gtgcccaggg aaaaggtcct cagactcggc
     4081 ctgccagaat aaggcgcgcc acttccgcgc gtgatgacag ggattgtgta tggggcctgg
     4141 gagttgaatc tgtaagtgaa agggcgga
//
LOCUS       DATIPF010000371         3876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1572170, whole genome shotgun sequence.
ACCESSION   DATIPF010000371 DATIPF010000000
VERSION     DATIPF010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572170"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VLL06_09350"
     CDS             <1..231
                     /locus_tag="VLL06_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31209.1"
                     /translation="LFIQQGKGVLIVGDALIGKPSGSVSLLPAEKYVDVGKAQEGLRR
                     LLKYNFDSLLVGDGASILTGAKQAVERSLQSS"
     gene            228..1013
                     /locus_tag="VLL06_09355"
     CDS             228..1013
                     /locus_tag="VLL06_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS31210.1"
                     /translation="MMTLRNGLSEELNRQGNEHFARGFYTDAYTCYAKALECDRLTGD
                     QRALAATLGNLGNICAVSGRRESAQTYYQEVLELQKILGDDKGIGTTLGNLGNLRADA
                     GEWDRAKAYYLEALDLMSKSHDEAGKAVLFSDLGLVARETKEYEQAIGYYEQSLILMR
                     RLGNEGGVADAWRMIGRTFLLQQRYDDAIACCQTSQSIAERSRDELRTGGARFVLVQC
                     YEEIGKLREAADLLELVVQMDRKYQLPKLEENTRRLAVLRAGL"
     gene            1052..1489
                     /locus_tag="VLL06_09360"
     CDS             1052..1489
                     /locus_tag="VLL06_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31211.1"
                     /translation="MSDEPSPAWASLKASLAPAFPQLSELETGGSLTMDLGPDGWLLE
                     LTPNGQLLCQYGMAIDDAMTLLSDGTPEDLGADEIAKQAKYYIQPAVSKYRAILLKSG
                     FSEESEITDEYVAARFERAVDVTNPAAVQDLMRWCVRTIGVAR"
     gene            1486..2556
                     /locus_tag="VLL06_09365"
     CDS             1486..2556
                     /locus_tag="VLL06_09365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSS31212.1"
                     /translation="MRKRIVTFDDLRDAITAYRLPRVLIAALELNLFAAIGTNAWTIP
                     ALARDVKVSERGLAILCRNLAMAGLLKKKGATYTNSRLGATALNADHPAYRRDYIRLI
                     ISHWADWGRLLESVKSGLPLDHDEPEEPDYRRRFTWAMHHRTLEIAPKIAGQINLRGA
                     QTLLDLGGGPGTYAMAFLAKNPTLHATVCDRPAALDVAREIASTHKAGPRLSYLPLDV
                     MTEGIPGSYDVIWYSNVLHIYSPKDNQSLFRRALASLNPGGRLLIQDAFLHDRNGLFP
                     EEASLFAVSMLLFTEGGNTYSAAETKTWLEDSGFERIKVLRMKKGMEDWEDGILEARS
                     PGTNVKMRARRTGSVKSLRSRS"
     gene            2892..3368
                     /locus_tag="VLL06_09370"
     CDS             2892..3368
                     /locus_tag="VLL06_09370"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HSS31213.1"
                     /translation="MTNERSRGITAMAGPSDRAAQTCLLIYDGQCRLCVTAKKGLEQL
                     ETHADAAPVRMVPYQSEEAEQALRESYRPGRPNAAFLVRPNGEIAHGLDAFLALLPGL
                     KGGRVLAAFLSCPLVKPFGYLLYWFVARYRYSIFGTVPLAGASENSHMPSRKTLQK"
     gene            3530..>3876
                     /locus_tag="VLL06_09375"
     CDS             3530..>3876
                     /locus_tag="VLL06_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31214.1"
                     /translation="MMIDQWLSNSGRRGKAFYVAVIASTLIGSSLPLTAWTGELSSLA
                     PEIPRNVVAAGVGYENADTSTITVKTYDAESGAILSDETYELNVREDAALADSQPRER
                     IFAGGVGPGADGLS"
BASE COUNT          829 a         1008 c         1204 g          835 t
ORIGIN      
        1 ttgttcattc aacaggggaa gggcgtactg attgtcggcg atgccttgat tggaaagccg
       61 tccggttccg tcagtctgtt gcccgctgaa aagtatgttg acgtggggaa ggctcaagag
      121 gggttacgcc gccttctcaa gtacaatttc gatagtctgt tggtcggcga cggggcctcg
      181 atcctcaccg gtgccaagca ggcggtcgaa cggtcgctcc aatcatcatg atgacactac
      241 gtaacggact ctccgaagaa ctcaatcgcc aggggaacga gcatttcgcg cgcgggttct
      301 atactgatgc ctatacctgc tatgcgaagg cgttggaatg cgaccggttg actggggatc
      361 aacgggcgtt ggccgccacg ctggggaatc ttgggaatat ctgtgcggtg agtgggcgtc
      421 gggaatcggc acagacctac tatcaagagg tgttggagct tcagaagatt ctcggagatg
      481 acaaggggat tgggacaacc ttgggcaatt tggggaacct gagggccgat gcgggggaat
      541 gggaccgcgc caaggcctac taccttgaag ccttggatct catgagtaag agtcacgatg
      601 aggcgggcaa ggcagtgctg ttttcggatc ttggtttagt cgcgcgtgag acgaaggagt
      661 atgagcaggc gattggatac tatgaacagt cgctgatttt gatgcggcgc ttggggaatg
      721 agggcggggt ggctgatgcc tggcgcatga tcgggcgaac gtttttgctc cagcagcgct
      781 atgacgacgc catcgcgtgc tgccaaacca gccaatccat tgcagagcga tcgcgcgatg
      841 agctgaggac cggcggcgct cgcttcgtgc tggtccaatg ttatgaagag ataggaaaac
      901 tgcgcgaggc agcggatctc cttgagctcg tggtgcagat ggaccgcaaa tatcagctgc
      961 caaaacttga agagaatacc cgacggttgg cagtcctgcg cgccggcctg taggggaagg
     1021 atggggagct cccccataga aggacccacg tatgagtgat gaaccctcgc cggcatgggc
     1081 ctccctcaag gcctcgctcg cccctgcatt tcctcaatta tcggaactgg aaaccggggg
     1141 ttcgttaacg atggaccttg gtcccgatgg atggctgttg gaactcacgc cgaacgggca
     1201 gctcttgtgc caatatggca tggctattga cgatgcgatg acgctcctgt ccgatgggac
     1261 accggaggat ctcggtgccg acgagatcgc aaagcaggcc aaatactaca tccaaccggc
     1321 agtctcaaag taccgggcga ttctgctgaa atcaggattt tctgaagagt ctgagatcac
     1381 cgatgagtac gtggcggctc gcttcgagcg ggcggtggac gtgaccaacc ctgcagccgt
     1441 gcaggacttg atgcgctggt gtgtgcggac gatcggcgtg gccaggtgag gaagcggatc
     1501 gtcacgttcg atgacttgcg cgatgcgatt acagcctatc ggttaccgcg ggttctgatt
     1561 gccgcgcttg agctgaacct ttttgcagcg attggtacga acgcctggac gattcctgcc
     1621 ttagctcgtg acgtgaaggt cagtgagcgg ggtctggcca ttctctgccg gaatcttgcc
     1681 atggccggcc tgctgaagaa aaagggtgcg acctatacga acagccggtt gggcgccacg
     1741 gccctgaatg cagaccaccc ggcctatcga agggactata ttcggctgat aatcagtcat
     1801 tgggctgatt ggggacgtct gctggaatca gtgaagagtg gattgccgct tgatcatgat
     1861 gaaccggagg agccggacta tcgacgtcga ttcacgtggg cgatgcatca ccggacgctg
     1921 gagatcgctc cgaagattgc aggccagatc aacctgcgtg gggcgcagac cctactcgat
     1981 cttggcggcg gtcccggcac ctatgcgatg gccttcctgg caaagaatcc cacacttcac
     2041 gccacggtgt gcgaccgtcc cgcagccctc gatgtcgcac gagagattgc gtcgactcac
     2101 aaggccggcc cgagactgtc ctacctgccg ttggatgtga tgacggaggg cattcccggc
     2161 agctacgatg ttatctggta ttcgaacgtc ctgcacatct attcgccgaa agacaatcag
     2221 tcgctgtttc ggcgtgcgtt ggcttccctg aatcccggag gccgtctatt gattcaggat
     2281 gcctttctcc acgaccgaaa tgggttgttt cccgaagagg cgagtctctt tgcggtctcc
     2341 atgctgttgt ttaccgaggg aggcaatacc tattcggcag ccgagacgaa aacctggttg
     2401 gaggactctg ggtttgaacg gatcaaggtc ttgcggatga agaaggggat ggaggactgg
     2461 gaggacggga ttctggaagc ccggtcccct ggaacgaatg taaaaatgcg cgcccgccga
     2521 acaggatcag taaaaagttt acggtcccgc tcatgacgct ggagaggaat agtgcgccgg
     2581 tctcgccctg cccgcccagg atgctcgtga ctgtggcaag gtctaacgcc aatcccacag
     2641 ttccatagat gacgcaagcc atggcggtcc aggaccatcc gatccacacg aggcctgcga
     2701 gagtgatagg acatgcgagg agatagagca tccatccgat tcggcttgcc tctgtgccag
     2761 gcggcatgag ttgaatggta ttcgcttcga tgaggcagag caccgcgagc aggccgataa
     2821 gcgtccacat gtgtcgcatg cagggaatga tactcgcgtc cgttgcacac agcaacagca
     2881 ggatgctgat agtgacaaat gagagatcgc gagggattac ggcgatggca ggtccatccg
     2941 acagagctgc tcaaacctgt cttctgatct acgacgggca atgccgcctc tgtgtcactg
     3001 ccaagaaggg gcttgaacag ctggagactc acgctgatgc cgccccagta cggatggttc
     3061 cgtatcagag cgaagaggcc gagcaggcct tgagggagag ttatcggcca ggtcgtccca
     3121 atgccgcttt cctggtccgt cctaacgggg agatagccca tgggctcgat gcctttctgg
     3181 ccctcttgcc gggtctcaag ggtgggaggg tcctcgcagc ctttttgagt tgcccgctgg
     3241 tgaagccatt cggctatctg ctgtattggt ttgtggcccg gtaccgatat tcaatcttcg
     3301 gcacggttcc gcttgcaggg gcctccgaga attcccatat gccgagtcga aaaacactcc
     3361 agaagtagtc aatttcccac tcgcaatacg atcttcttcc tagtctcatc accgtccatt
     3421 ttctgttcaa cattctcccc cctctttcga tgggttttct ccccacagtg gggaatttac
     3481 gctgaaactg gccaatgcga gactccacac aagcgaggga ggaccgcaca tgatgatcga
     3541 ccagtggctg agtaatagtg ggcggagagg aaaggccttc tacgtggcag tgatcgcgtc
     3601 tactctgatc ggctcaagcc ttccactgac agcttggact ggagaactct cgtctctcgc
     3661 gccggaaata cccagaaatg ttgtggcagc tggtgtcggg tatgagaatg cggacacgtc
     3721 tacgattacc gtgaaaacct acgatgccga gagcggcgcg attctttccg atgaaactta
     3781 tgagttaaac gtgagagagg acgcggcgtt ggcggacagt caaccgcgtg agcggatttt
     3841 tgcgggcgga gtcggtccgg gagccgatgg actttc
//
LOCUS       DATIPF010000372         2876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_162191, whole genome shotgun sequence.
ACCESSION   DATIPF010000372 DATIPF010000000
VERSION     DATIPF010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162191"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="VLL06_09380"
     CDS             complement(<1..885)
                     /locus_tag="VLL06_09380"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSS31215.1"
                     /translation="MNGSITSASFNCSLLESRRFSIIPMVIFLSCGFLVLTIGHPPLA
                     MSAFPSDDMALVPAGEFLMGNPAGSDGLPDEQPQRLVYTGAFRIDRYEITHDGYARFV
                     RMTGHRAPANSNPAATLWESNAPISGIGRHPVINVSWDDAVTYCAWIGKRLPTEAEWE
                     KAARGTDGRRYPWGNEWDFTKANSASYWAGRTIDDFESGVSWDAFWIKGEGAKISKEK
                     GIKGEVLTTPIGSFPEAVSPYGLYDMAGNVAEWVQDWYNPNYYKEAPLSDPQGPLRGA
                     IKAMRGGSWLKPAISLRTS"
     gene            1390..1713
                     /locus_tag="VLL06_09385"
     CDS             1390..1713
                     /locus_tag="VLL06_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31216.1"
                     /translation="MSEAKDKTIALNVRLKPVDHSNLPHQANYSNVGVAQGIAYLDIG
                     FIEPGLLGAIARTTRDGQAAPKAIDGQLVTRVAMGVDVLVRLHQQIQQVLVGLRDVQE
                     PNPKS"
     gene            1850..>2876
                     /locus_tag="VLL06_09390"
     CDS             1850..>2876
                     /locus_tag="VLL06_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HSS31217.1"
                     /translation="MPADIGKALLPPTEPAPPSAAPEITDTGLICLLILARFYDLPAD
                     GAQLRHQFAQSGEVLSDTGLLRAAKHLGLKAGVLTSNWNKLGGIPLPAVAKRTDGRYV
                     VLAKLEGEKALVQDPTAARPLILSREQFESAWTGQLLLFTKRANLRVQDLKFDFTWFI
                     PAIVKYRKHLGEVLVASFFLQLFALLTPLFTQVVIDKVLVHKGFTTLYVLAVGMIALA
                     IFDAILGGLRTYLFSHTTNRIDVGLGAQLFRHVLALPLAYFEARRVGDTVARVRELEN
                     IRQFLTSHSVTVVLDLFFTAVFLAVMWFYSPILTLVVMGSLPLYAVLSVVITPAIRAR
                     LHEKFNRG"
BASE COUNT          602 a          791 c          792 g          691 t
ORIGIN      
        1 gcttgtgcga aggctgatag ccggtttaag ccaggatccg ccccgcatcg ctttgatcgc
       61 accgcgaaga ggtccctgag ggtctgagag aggggcttcc ttataatagt tcgggttata
      121 ccaatcctgc acccattcag cgacgttgcc tgccatatcg tagaggccat agggacttac
      181 tgcttcggga aaacttccaa ttggtgtcgt gagcacttct cctttgatgc ccttctcttt
      241 tgaaatcttc gcgccctcac ccttgatcca gaacgcatcc cagcttacac cgctctcaaa
      301 gtcgtcaatg gttcgtccgg cccaatagct ggcgctgttg gccttcgtga agtcccattc
      361 gtttccccaa ggatagcggc ggccatcggt gccgcgcgcc gccttttccc attctgcctc
      421 ggtcggcaga cgcttgccga tccaggcgca gtaggtcacg gcatcatccc agctgacatt
      481 gatcacagga tgtctgccaa tgccggatat cggtgcattg ctttcccaca atgtggcagc
      541 agggttcgag tttgccggtg cacgatgccc ggtcatccgg acaaatcgtg catagccatc
      601 gtgggtgatt tcgtagcggt cgatccggaa cgctccggtg tagacgaggc gttgcggctg
      661 ttcgtcggga aggccatcgc tgccggccgg attccccatc aaaaattctc cggcaggaac
      721 caacgccata tcatccgaag gaaacgcact catggccaat gggggatggc cgatggtcag
      781 aacaagaaac ccacatgaga gaaagatcac catagggata attgagaatc gtcgagactc
      841 caataggctg caattgaatg atgcggatgt gatcgagcca ttcatgagag aggagtcatg
      901 aggttttaac agtatatgcc aagacgtgga tgatagagga aatcgagttg gctcgtccat
      961 cctgcccctc agaaaaaatg tgcagatcgt aacgagctgc cctatcgtct gatgtggtcg
     1021 aacgctacat ccatggggca tgaaaccagc gggtatttcg atcgtacccg agtacaacgg
     1081 ctcctgtttg gagggcctcg ccgtcagatc catcagtggc catccaggac ctatcaatgg
     1141 agtacttcgg ttccagccat ggataggagc aatctctcag actctatcgg gtccggtccc
     1201 gtctgcgcaa tgtcctgcac gatcgtaatg aatgatggga gacaataatt cacttgacag
     1261 gaacactaag caatagttac attcggctgc cttgccatta ggaaagcata aacaactgaa
     1321 gatttccatt tgtctgcgca tcttcctctc accagcgtca gcgatcttcc atagaaggag
     1381 ttgagtttca tgagtgaagc caaagacaag actattgctc tcaacgtgcg ccttaagcct
     1441 gtggaccatt ccaatctgcc gcaccaggca aactacagta acgtgggtgt ggcacaaggg
     1501 attgcctatc tggacatcgg gttcatcgag ccgggcttgc tgggtgctat tgctcgaacg
     1561 acgagagacg gccaagccgc cccgaaggcg atcgatggcc agctcgtcac tcgcgtagcg
     1621 atgggtgtgg atgtgttggt gcgactgcat cagcagattc agcaagtatt ggtcggcctg
     1681 cgtgatgtgc aggagcccaa cccaaaatca taagttgctg tgactggaga gccgatgacg
     1741 aagcaaggtt tcatcgtcaa tattaatcgg atgggtgacc tggctcagat gcacttgcgt
     1801 ccgatgaaat tgttttcgcc gcttgtccgg ggtcccctaa gatgacgcca tgccagcaga
     1861 catagggaag gcccttcttc ctccgactga accagcaccg ccatctgctg cgccggaaat
     1921 tacggacacg ggtctgatct gtctgttgat tttggcccga ttctacgact tgccggcgga
     1981 tggcgcacaa ctgcgccacc agtttgccca atcgggcgaa gtcctctctg ataccggtct
     2041 tctgcgtgcc gccaaacacc tcggtctcaa ggccggtgtt ctgacttcca attggaacaa
     2101 gctcggtggg attcctcttc cagctgtcgc aaagcggacg gatggccgtt atgtcgtact
     2161 cgcgaagctc gaaggagaga aggccctcgt ccaagatccc actgcggcgc gcccgctgat
     2221 tctctctcgc gagcagtttg agtcggcctg gacgggacaa ttgctgctgt tcaccaagcg
     2281 ggcgaatctt cgtgtgcaag atcttaagtt cgatttcacc tggttcattc ctgccatcgt
     2341 caaatatcgg aagcatctgg gagaggtgct ggtcgcgtca ttcttcttgc aactcttcgc
     2401 actgctgacg ccgctcttca cccaagtcgt catcgacaag gtgctcgtcc ataagggctt
     2461 caccacgctc tatgtgctgg cagtcggcat gattgccctg gccatcttcg atgcgatctt
     2521 gggtgggctg cggacctatc tgttttctca tacgacgaac cggattgacg tcgggctggg
     2581 cgcgcagttg ttccgccatg ttttggcgct ccccctggcg tattttgagg cgcgccgggt
     2641 gggggatacg gtggcccggg tccgtgaact ggaaaatatc cgccagttct tgacgagcca
     2701 ttcggtgacc gtcgtgctcg atctgttttt taccgcggtg tttctcgcag tgatgtggtt
     2761 ctacagccct atcctcacgc tggtcgtcat gggttcgttg cccctctatg ccgtcttgtc
     2821 cgtggtcatt acaccggcga ttcgggccag gctgcatgaa aaattcaatc gtggtg
//
LOCUS       DATIPF010000373         3286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_515349, whole genome shotgun sequence.
ACCESSION   DATIPF010000373 DATIPF010000000
VERSION     DATIPF010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515349"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..902)
                     /locus_tag="VLL06_09395"
     CDS             complement(108..902)
                     /locus_tag="VLL06_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HSS31218.1"
                     /translation="MVERFEVALNTPAGRLTTAIDVPTGFIPITAIVPVTRRLGDEAA
                     QLEIQQATAAGQTISCRKGCAACCRMLVPVSAPEAFALREYVEQLPTERRTPLLNRLN
                     DTKERLTREGLWDRLTDVAEASRPVPDEELDPINQSYYALRIPCPYLEDETCSIYEAR
                     PAACRELLVTSPAELCQDMVQNPISPLPISMRIGSILGLLWGTLTSSPPRLIPLPMAL
                     EWTERHEADSRTTWPGSSLLDQVLDNMWRFLSQEFTNRRGTDGKSR"
     gene            1120..1482
                     /locus_tag="VLL06_09400"
     CDS             1120..1482
                     /locus_tag="VLL06_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31219.1"
                     /translation="MALSGHVVGLLKEYMRDLVEQAKQETAAHASFGFSATPYRSDQA
                     LSDLLAILDDRIESEGMQVGLPDEFLHQMWGLCNEARAQVTERVWLDINSSNQPSSKA
                     RVRELTYRALLAVIETSG"
     gene            complement(1687..2499)
                     /locus_tag="VLL06_09405"
     CDS             complement(1687..2499)
                     /locus_tag="VLL06_09405"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HSS31220.1"
                     /translation="MELTPFFFGLYKFVKYGLYPLTWVVFLLSVAMVLALFPFSPTRL
                     RWVRLLVTSSLLLLLMVSSPLIGNPLIGSLESWYQPPSLTPSDQFDAIVILGGGLEEK
                     GSLRPTVELSPHSKNQTTCGVDLFQRGYAPTLVLTGGDTRVFGTGPQEAVEMKRWAVR
                     LGVPESAMMIETEARNTYENATGTKRLLGPASILLVSSASHLPRAVPVFTKQGFHVTP
                     APCGYLSQNLPRERWDTVTIFDFLPSNTALQNTTETVTEVAGIVVYWLAGKL"
     gene            2582..3061
                     /locus_tag="VLL06_09410"
     CDS             2582..3061
                     /locus_tag="VLL06_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HSS31221.1"
                     /translation="MTHACRYWLMKSEPGVFSIEDLARSPKQTTCWDGVRNYQARNFM
                     REMAVGDRVLFYHSSAEPPTVVGIAEVVRTAYPDKTQFDKASHHYDPASDPSAPRWDM
                     VDIRYRQTFKASLPLDRLRQEPKLKNMVLLQKGSRLSVQPVTEAEWAVVLKLAGAKD"
BASE COUNT          717 a          882 c          998 g          689 t
ORIGIN      
        1 gatccagatc tccggcacca acaccccttc tagatctaaa cagacgatga cgggactctt
       61 catgacggct cttctcctct taattggacc atcggcgtga tgcgacgtta tcggctcttg
      121 ccgtccgtcc cgcgcctgtt cgtaaattcc tgactcagaa accgccacat attatcgagg
      181 acctggtcca acagagatga gccaggccag gtcgttcggg aatctgcttc gtgtcgctcg
      241 gtccactcca atgccattgg gagcgggatg agccgaggag gcgagctggt caatgtcccc
      301 cagagcagcc ccaggatgga accgatccgc atcgagatcg gcaggggact gatcggattc
      361 tgcaccatat cctgacagag ttcggccggt gaggtgacga gaagctcccg gcaggcggct
      421 gggcgagcct cgtatatcga gcaggtctca tcctccaaat aggggcaagg gattcgtagc
      481 gcatagtaag actggttgat ggggtcgagt tcttcatccg gcacgggcct ggaggcctct
      541 gccacgtcgg tgagtcggtc ccagagaccc tcgcgcgtga ggcgttcctt cgtgtcgttg
      601 aggcgattga gtagcggggt ccgccgttcc gtcggcaact gttccacata ttctcgcagc
      661 gcaaacgctt ccggcgcgga gactggaacc agcatgcggc aacaggcggc gcaacccttc
      721 cggcaggaaa ttgtttggcc tgctgcggtg gcctgctgga tttccaactg tgcggcttca
      781 tccccgagac gacgcgtgac cgggacaatc gcggtaatgg ggatgaatcc agtcggcacg
      841 tcaatggctg tagtcagccg tccagcggga gtgttcagcg ccacttcaaa tcgttcgacc
      901 atcacaagcc tgttctctgt acccgttcgt atcggcatca tagagaaccg ttgatcggct
      961 ggtcaatcaa cggccctaac aggacagccg tgggagacag gtgggactgg ttgatttggt
     1021 ttatcgggtt tgttgagttc gtctggttag tctggttcaa ccagccggtt cttgctccct
     1081 ctctgacaga tccgcataat gtgcgcgaag ggggatacca tggcattgtc cggtcatgtg
     1141 gtgggcttgc tcaaggagta catgcgggat ctggtggagc aggcgaagca agagacggcc
     1201 gcccatgcct cctttggatt ttccgcgaca ccctatcgat cggatcaggc gctatcagat
     1261 ttgttggcca ttctggacga tcgcatcgaa tccgaaggga tgcaagtcgg cttgccggac
     1321 gaatttctcc accagatgtg ggggctctgc aacgaggccc gcgctcaggt gacggaacgc
     1381 gtgtggttgg atatcaacag cagtaatcag ccatcgtcga aggccagggt ccgagagttg
     1441 acctatcgcg cgctgctagc tgttattgag acttctggtt agctggctgt ggaaaaacta
     1501 ttttggtggg ctaaagctta gacggtcagt acatgcggga caacgggaga acattcccgc
     1561 aggatgctca aaaaggccgt ccagcaaggc cgcagcgagt gaaggcccga ggcataccct
     1621 ctggggtacg ttgagggtct gaacgatgcg agaacgctgc tggcgggctt tttcagcatc
     1681 ctgccattag agtttccctg ccaaccagta gacgactata cccgccacct ccgtgaccgt
     1741 ctccgtcgtg ttttgtaacg cggtattgct aggcaaaaaa tcgaatatag tgacggtgtc
     1801 ccaacgttct cgtggcaggt tctgtgagag gtaaccgcag ggggccggcg tgacgtggaa
     1861 gccctgcttg gtgaacaccg ggacggcccg tggcagatgg ctcgctgaac tcacaaggag
     1921 aattgatgcg ggtccgagca gccgtttggt ccccgtcgcg ttctcatagg tgttgcgggc
     1981 ttctgtctca atcatcatgg cggactcggg aacgccgagg cgaaccgccc atcgtttcat
     2041 ttcgaccgct tcttgggggc ctgtgccgaa gaccctggta tctccccccg tcaacaccaa
     2101 cgtcggggca tagccccgct ggaagagatc cacgccgcac gtggtttgat ttttggaatg
     2161 gggagacaat tcaacggtcg gccggagcga gcctttttct tccaggcctc cccccaaaat
     2221 cacgatcgca tcgaattgat cggatggcgt caacgacgga ggttggtacc aggattccag
     2281 cgagccgatc agcgggttgc cgattagggg actcgatacc atcaagagaa ggagaaggga
     2341 ggatgtgacg agcaggcgta cccaccgcag gcgtgtcggc gagaacggga agagggccaa
     2401 gaccatcgcc acgctgagga ggaacaccac ccacgtcagt ggatagaggc cgtacttgac
     2461 gaacttatac agaccaaaga agaatggagt gagttccatc aggagatctc agtcgaatag
     2521 gaagcctgga cgacagcata cacggagtga accgaatgac caagcaggag ggggtaatgc
     2581 gatgactcac gcgtgccgct attggctgat gaaatcggaa ccgggtgttt tttcgatcga
     2641 ggatcttgcg cggtctccca agcagaccac ctgttgggat ggagtacgga actatcaggc
     2701 gcgcaacttc atgcgtgaaa tggccgtcgg agaccgagtg cttttttatc atagtagtgc
     2761 cgaaccgccg actgtggtgg gaattgcgga ggtcgtgcgg acagcctatc ctgacaagac
     2821 acagttcgat aaggccagcc accactatga cccagccagt gatccctctg ccccacggtg
     2881 ggatatggtg gatatccggt atcgtcagac gttcaaagcc agtctgccgc tcgaccgact
     2941 acggcaggaa cccaagctga agaacatggt cttgttacaa aaaggttccc gcctctccgt
     3001 ccagccggtc acagaagccg aatgggctgt ggtcttgaaa ctagccggag ctaaagatta
     3061 gctagcagcg caaccaatgc aggttcgctg attaaatggt ttcaagtctg aggcggcggg
     3121 gaatacatcg gcaggctgct caaaaaggcc gttcagcaag gccgcagcga gcgaagaggc
     3181 gaggaggtac ctaccgcact tcgtggggcc gttcgccctt cacatgattc ctggcgaatg
     3241 gaaaatcccc ttgagtgtct tgggtccttc aaggatttat gctcac
//
LOCUS       DATIPF010000374         3073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_957730, whole genome shotgun sequence.
ACCESSION   DATIPF010000374 DATIPF010000000
VERSION     DATIPF010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957730"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VLL06_09415"
     CDS             complement(<1..303)
                     /locus_tag="VLL06_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31222.1"
                     /translation="MNEMISFMLGFALVFSSPVGEGFFANWQVPFVGSYSLTIFDALV
                     LPVGIIAFCLYGFRVKPQERFLYGSLSLVILSRIVSLVAGGHVAIEQSVSVFRYVEA"
     gene            complement(342..1148)
                     /locus_tag="VLL06_09420"
     CDS             complement(342..1148)
                     /locus_tag="VLL06_09420"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSS31223.1"
                     /translation="MSEFNARAYWEDRIASNLGLHGVGYLGYGPAYNDWMYRVRSVIF
                     RRTVRELGVKLSASSVLDIGSGTGFYVRMWRQLGAKRVTASDLTSAAIEHLRETIPDI
                     RAGQLDIGRPVTSALGSERFDVISAMDVLFHIVDDAAFDQAIANIAGMLVPNGWFLMS
                     DNFVHGQAQRINHQVSRTLDDITLVLQRSGLEPVKRTPMFVLMNAPTDTSSRWPLLAW
                     RAAMVPVRLVNAIGHVQGALLFPLELLLTSVMSESPTTELLICRKTVTGG"
     gene            complement(1145..2080)
                     /locus_tag="VLL06_09425"
     CDS             complement(1145..2080)
                     /locus_tag="VLL06_09425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSS31224.1"
                     /translation="MKQHVFAPLRFGNLADLIRESPHERPAGEWPKLSIVTPSYNQGS
                     FLERTIRSVLDQAYPNLELIVIDGGSTDNTLEIIRRYERHITYWVSEKDSGQSDALNK
                     GFKRATGEFVGWQNSDDVYLPGSFFEAVDALRQNPEIDVAFANRLDVDEDDRITAECR
                     FTPFSRIGYWYEGMSMSNQSAFWRRSVFERVGYFDVDLHAAMDYEFFLRCAFNGIRFR
                     HFRRYWGAIRRHRASKGSVLWPVALKGDCDLIDARYGRKKVLNVPLRIYSTLRRVIYY
                     TLRGDFDYLTHGIIRRSAAAIHPPLSPAARERSVQ"
     gene            complement(2082..2969)
                     /locus_tag="VLL06_09430"
     CDS             complement(2082..2969)
                     /locus_tag="VLL06_09430"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HSS31225.1"
                     /translation="MDAGPVLNLLRWYGLHSPIPRGKRLLPRIATAILGRLEGRILVS
                     TVDGRLFWVDLDDVLYRDVYFMGFYERRVTNLASRLLRAGDVAFDVGANMGWYTTLFA
                     KIVGAAGQVHSFEPSPAIFDRLQTNLVANGSPTWVLANQFALGPEETEAVIHTFAGLT
                     HGHASLSELSRDDFSSTKCMVTTLDSYAQRHGVSKIDLIKCDVEGAELGVLEGSRNIV
                     KEMPPPMWILEINDETSKAFGYTPADMLGVLSTFGYEFYAIRGKLVRVGVRSEYTHGD
                     NVLAIVPAAHESRLKAAIS"
BASE COUNT          731 a          892 c          841 g          609 t
ORIGIN      
        1 cgcctcgacg tacctgaaaa cgcttacgga ttgctcgatt gctacgtgac caccagccac
       61 aagcgacacg atacgcgaca gaatcacaag actcagggac ccgtagagga atcgctcttg
      121 tggcttgacg cgaaaaccgt agagacaaaa agcgatgatg cccacgggca ggaccagcgc
      181 gtcaaaaatc gtcaggctat acgagccgac gaaaggaacc tgccagtttg cgaagaagcc
      241 ctctcccaca ggagacgaaa acaccagcgc gaagccaagc atgaacgaga tcatttcgtt
      301 catgcgtgag ggacgccaca ctggccgatc gaaactccgg gtcagccccc cgtaactgtc
      361 ttgcggcaaa tcaggagttc ggtggtcgga ctctcgctca taacgctggt cagcagcaac
      421 tcgagcggaa acagcagggc cccttgaacg tggccaattg cattcacgag ccggaccgga
      481 accattgctg cccgccaagc caatagaggc catcttgagc tcgtatcggt gggagcattc
      541 atgaggacga acatgggggt ccgcttgact ggctcgaggc cggaacgttg caacacgagg
      601 gtgatgtcgt cgagcgtgcg acttacttgg tggttgatgc gctgcgcctg cccgtgaaca
      661 aaattgtccg acatcaggaa ccaaccgttc ggcacgagca tgccggcgat gttggcgata
      721 gcctgatcaa atgccgcgtc gtccacgatg tgaaacagga catccatagc actaatcaca
      781 tcaaaccgct cactcccaag ggctgacgtg acggggcgac cgatgtctag ctgaccggcg
      841 cggatgtcgg ggattgtctc acgtaagtgc tcgatcgcgg cgctcgtcag atccgacgcg
      901 gtgacgcgct tcgcgcctag ttggcgccac atgcgcacat agaagcctgt acccgagccg
      961 atatcgagca cacttgacgc tgacagcttg actccgagct cccggacagt cctgcgaaaa
     1021 atcacggacc gaacccggta catccaatcg ttgtatgcgg gaccatagcc tagatagcca
     1081 acgccatgca accccagatt ggaggctatc cggtcctccc aatacgcacg cgcgttgaac
     1141 tcgctcattg caccgatcgt tcgcgtgcgg ccggcgagag tggcggatga atcgcggctg
     1201 cagaccggcg aataatcccg tgtgtcaggt agtcgaaatc accgcgcaag gtgtagtaga
     1261 tgacccgccg gagcgttgag taaatcctga gcggaacatt gagaaccttt tttcggccgt
     1321 agcgcgcgtc gatcagatcg cagtcgcctt tcaacgccac cggccacaaa acgcttcctt
     1381 tcgaggcgcg atgccgtcga atggctcccc agtaccttct gaaatggcga aaacgtattc
     1441 cgttgaacgc gcatcggagg aagaactcat agtccattgc cgcatgcagg tctacgtcaa
     1501 agtagccgac cctctcgaaa acgcttcgtc gccagaatgc gctttgattg ctcatcgaca
     1561 tcccttcgta ccagtatcca atccgtgaga acggagtgaa cctacactcg gccgtgattc
     1621 tgtcgtcctc gtcaacatcg aggcggttcg cgaatgccac gtcgatctcg ggattctgtc
     1681 gtagtgcgtc gacggcctcg aagaacgacc cgggaagata cacatcgtcg gaattctgcc
     1741 agccgacgaa ttcgccagta gcgcgtttga aacccttatt caaagcatcg ctctgcccgc
     1801 tgtccttctc gctcacccaa tacgtgatgt ggcgctcgta tcgccgaatg atttcaagtg
     1861 tgttgtcagt cgaaccacca tcgataacga tcaattcaag gtttgggtag gcctggtcca
     1921 aaacgctgcg gatcgtgcgc tccaggaatg acccttgatt atacgatggc gtgacgatgg
     1981 acagcttcgg ccactcgccg gcgggccgct catggggaga ttcccgaatc aggtccgcaa
     2041 ggttcccaaa gcgcaatgga gcgaagacat gctgcttcat cctatgagat cgctgccttc
     2101 aaccgagact catgggctgc aggaacgatt gccaggacgt tatccccatg ggtgtactcg
     2161 cttctgactc cgactcgcac cagttttccc cgtattgcgt aaaactcgta gccgaaagtc
     2221 gataacaccc cgagcatatc tgcgggagtg taaccaaatg ctttcgatgt ctcgtcattg
     2281 atctcgagga tccacatcgg aggcggcatc tccttcacaa tatttcggga cccttcgagc
     2341 acgccgagct ccgccccttc cacgtcgcat ttgataaggt ctatcttgga gacaccgtgg
     2401 cgctgagcgt atgagtcgag ggtggtgacc atgcacttcg tcgatgagaa gtcatctctg
     2461 gagagctcgg acagggaggc atgaccgtgg gtcagaccag cgaaggtgtg aataactgct
     2521 tcggtttctt caggcccaag ggcaaactga ttggcgagaa cccacgttgg tgacccattg
     2581 gcaacaagat tcgtttgaag ccgatcgaaa atcgccggac tgggctcaaa cgaatgcact
     2641 tgaccagctg cgccaacgat tttcgcgaag agcgtggtgt accaacccat gttggccccg
     2701 acatcgaacg ccacgtcacc ggcccggagc agacggcttg caaggttggt aacgcgtcgc
     2761 tcgtagaaac ccatgaagta gacgtcacgg tagagaacat cgtccaggtc gacccagaaa
     2821 aggcgcccat cgaccgtgct tacgaggatc cgtccttcga ggcgaccaag tatcgcagtt
     2881 gcgatacgcg gcaacaaacg ctttccacgg ggaatcggag agtgaagccc ataccaccgc
     2941 agcagattca agactggacc agcatccatg gaagtactct tacgggttaa taacgaaaca
     3001 tgactgtcct tcatccgagt gatctcatcc aaccaattct gcgggtttca ccggcttgag
     3061 ctgacggacc agc
//
LOCUS       DATIPF010000375         2549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_780254, whole genome shotgun sequence.
ACCESSION   DATIPF010000375 DATIPF010000000
VERSION     DATIPF010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780254"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..893)
                     /locus_tag="VLL06_09435"
     CDS             complement(105..893)
                     /locus_tag="VLL06_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HSS31226.1"
                     /translation="MLVGFYQFAPVFGDVSHNLDKVVDTLDRAEADLIVLPELFASGY
                     QFVSQQEMTTLSESVPDGPTTQRLIDLAKRRRMVIVAGLAERVGAACYNSAVVVGPSG
                     FIGCYRKTHLFFEETLFFTPGDTGFQVWDVGMAKIGVMICFDWYYPEAARTLALKGAE
                     IICHPSNLVLPNCPDSMPVRCLENRVFAVTCNRTGSEARGGKDQLTYIGNSEVVAPRG
                     AIMHRAPQDQEELYIVDINPADARNKALTPYNDLLRDRRESLYR"
     gene            complement(966..1310)
                     /gene="smbP"
                     /locus_tag="VLL06_09440"
     CDS             complement(966..1310)
                     /gene="smbP"
                     /locus_tag="VLL06_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HSS31227.1"
                     /translation="MKMRIHRGALVGVAVAALVGFPLFSGLALAADKHAAEALEHAKE
                     AVAHGKQGHADVLVKHAEEALKHAGAAGKNPHVDEGIKHLKEAVEHGKAGHADVATQH
                     AEGAVTHLSEVK"
     gene            complement(1405..2283)
                     /locus_tag="VLL06_09445"
     CDS             complement(1405..2283)
                     /locus_tag="VLL06_09445"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HSS31228.1"
                     /translation="MTHELLIDTEQLQQRLGQPDLVILDVRGRAAYEFGGHIPGAVHS
                     TWHEYSDPAAVPKGLLDPDRGRMEQRIRALGISQDSEVVIYSNPFDNWGDEGRMFWML
                     EYLGHTRLRILDGGWVKWVQEKRPFEHGCVTPKLGTFSVKPVAAVIAAKDELKHLVKQ
                     PHPGTVIVDARSLEEYLGKEVSGIPRPGHIPSALHLAWNGFLHANATVKDLPTVKESL
                     AEKGLKQDQEIICYCTGGVRSAWLYFVLKLAGYRKVRNYPGSWWEWSRDFACPVEKDA
                     KGLQHILNVDPQVRPS"
     gene            complement(2393..>2549)
                     /locus_tag="VLL06_09450"
     CDS             complement(2393..>2549)
                     /locus_tag="VLL06_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HSS31229.1"
                     /translation="TEEGYADLTDIVASIAKRHAGGRVLSSLEGGYHLKALATSIDRH
                     IQALLAA"
BASE COUNT          549 a          779 c          668 g          553 t
ORIGIN      
        1 tccagcaagg ccgcagcgag tgaagggccg aggcgtaccc tctggggtac gttgagggtc
       61 tgaacgatgc gagaacgccg ctgacggact gtttcagcat cctgctagcg atagagggat
      121 tcgcggcggt cacgaagtag atcattgtag ggcgtgaggg ctttgttccg cgcatcagca
      181 ggattgatgt caacaatata gagttcttcc tggtcctggg gcgcgcgatg cataatcgct
      241 ccgcgtggag cgacaacttc gctgtttcca atatacgtca gctggtcttt tccacctcgc
      301 gcttcgctgc ctgtccgatt acaggtcaca gcgaacactc gattttccaa gcaccgaacg
      361 ggcatggagt ccgggcagtt cggcaacacg aggttcgagg gatggcagat gatctctgct
      421 cccttgagcg ccaacgtgcg agccgcctcc ggatagtacc agtcgaaaca gatcatcacc
      481 ccgatcttgg ccatcccgac atcccacacc tgaaatcccg tgtcgcctgg ggtaaagaac
      541 agcgtctctt cgaaaaacag atgcgtctta cgataacacc caataaaccc ggacggaccg
      601 acgaccacgg cggagttata gcaggccgcc ccgacacgct ctgcaagccc tgcaacgatc
      661 accatccggc gccgtttcgc aaggtcgatc agccgttgcg tcgttggccc gtccggaacc
      721 gactcagaca gcgtggtcat ttcttgctgt gacacgaact ggtaacccga agcaaataac
      781 tcgggcaaga cgatcagatc ggcctcagcc cggtcgagtg tgtccacaac cttatcaagg
      841 ttgtgcgata catcgccgaa gactggagcg aactgataaa acccgaccag catgatcgtc
      901 atatccccag aacaaaacgg gcagggttgc ccctgcccgt tcaaaaagat gactcgccga
      961 ataacttact tcacttcgga caaatgggtg accgcgcctt cagcatgttg tgtggcgaca
     1021 tcggcgtggc cggccttgcc atgctcaaca gcttccttga gatgcttgat cccctcatcc
     1081 acatgcggat tcttcccggc cgccccggca tgcttgagtg cttcctcagc atgttttacc
     1141 aggacatcgg catgtccctg tttgccatgc gcgacggctt ccttggcatg ctccaaggct
     1201 tcagccgcgt gcttgtccgc tgcaagagcg agcccgctga acagcgggaa accgacaagg
     1261 gcggcaactg ccacaccaac cagagctccg cgatggattc tcattttcat gggttcctcc
     1321 tcaatggtta aggttcatgc aacgggtgaa cgatatgggc acgggattcg agtggtttca
     1381 ccttacacag ccatttttac actgtcaaga aggcctcacc tggggatcga cgttcagaat
     1441 gtgctgtaat cccttggcgt ctttttccac cggacaggca aaatctcgac tccactccca
     1501 ccaggatcca ggatagttgc ggaccttccg atacccggcg agtttgagga caaaatacag
     1561 ccaggctgaa cggacaccgc cggtgcaata gcagataatc tcctgatcct gtttcagacc
     1621 tttctcggcg agactctctt tgaccgttgg cagatctttc accgtcgcgt tcgcgtgaag
     1681 aaatccgttc cacgccaggt gaagcgcgga gggaatgtgg ccgggtcgcg gtatccctga
     1741 cacctctttc ccaagatatt cttcgaggct gcgcgcatcg acgatcacgg tgccaggatg
     1801 cggctgcttc acaaggtgct tcaattcgtc cttcgcggcg atgacagcgg ccaccggctt
     1861 gacgctgaag gtccccagct tgggcgtcac gcacccatgt tcgaacggcc gtttttcctg
     1921 cacccacttg acccacccgc cgtcgaggat acgcagacgc gtgtgtccca gatattccaa
     1981 catccagaac atgcgacctt catctcccca attgtcaaac ggattggaat agatgaccac
     2041 ctcgctgtcc tgactaatac ccaaagcacg aatcctttgt tccatacgtc cgcgatcagg
     2101 atccagcaat cccttcggca cggcagctgg atcgctgtat tcatgccacg tcgaatgcac
     2161 cgcccctgga atatggccgc cgaactcgta ggccgcccgc ccacgcacgt cgagaatgac
     2221 caggtctggc tgtcctaatc tctgctgaag ctgctcggtg tcaattaata actcgtgcgt
     2281 catgtcgttc cttgtgtcgt gtgagccgtc aaccatcaac cctcagttcc agccttgcaa
     2341 cctattctat ccgactgacc gctgagagct ggttgccgtc gctcgtaggt cttcatgcag
     2401 ctaagagcgc ctgaatatgg cgatcgatcg aggtggccaa ggccttcaga tgataccctc
     2461 cttcaagcga cgaaagcacg cgcccgccgg catggcgctt ggcaatactg gccacgatgt
     2521 cggtgaggtc cgcatagccc tcttctgtc
//
LOCUS       DATIPF010000376         4360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_246748, whole genome shotgun sequence.
ACCESSION   DATIPF010000376 DATIPF010000000
VERSION     DATIPF010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246748"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VLL06_09455"
     CDS             <1..199
                     /locus_tag="VLL06_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HSS31230.1"
                     /translation="SKSCVELKSLKMYMLAYRSLGIFYENAVNRILHDVVKACRPVWA
                     KVTGEFAARGGLRSLIETKYP"
     gene            245..1066
                     /locus_tag="VLL06_09460"
     CDS             245..1066
                     /locus_tag="VLL06_09460"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HSS31231.1"
                     /translation="MATYAIGDVQGCNDPLQRLVEQIQFDPVSDRLWFVGDLVNRGPD
                     SLKVLRYIKSLGTSAVVVLGNHDLFLLAVADGIVTPSAQDTLQPILDAPDRDELLGWV
                     RQQRLLYHESSFVVIHAGLLPQWSVREAEDLAREVETGLRSPYYKDVLRALYPSKHLQ
                     WSSALTGPIRLATIIKVLTRLRACSVDGLMESSYSGPPAHIPAGFVPWFRIENRERRE
                     TTIICGHWASLGLHCEKNVLAIDSGCVWGRQLTAIRLEDRKVFQVDCNHIERDGD"
     gene            1131..1598
                     /locus_tag="VLL06_09465"
     CDS             1131..1598
                     /locus_tag="VLL06_09465"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HSS31232.1"
                     /translation="MPGRTPHPRRDPSGHSYGLSEPKPTPFSVDQWHTSEDYLYGIDL
                     YNFAYWWECHEVFEGLWHAVGHHSEQGNFFQALIQVAAANLKQFLGNEQAAQKLARSG
                     LARLQNLPQLYMGIDVIALSEACRAISTPLANSTPYQIRLSLETHSSPVNLKS"
     gene            complement(1803..2591)
                     /gene="panB"
                     /locus_tag="VLL06_09470"
     CDS             complement(1803..2591)
                     /gene="panB"
                     /locus_tag="VLL06_09470"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HSS31233.1"
                     /translation="MTIPEFQRHKRDKKKLIVVTAYDALFTRIVEQAGIQTILVGDSL
                     GVVVQGKANTLSVTMDDMLYHTKLVAGAAQQALVIGDMPFMSYQASQEDARRNAGRFL
                     QAGAHAVKLEGGAAVVDQVVAMTNIGIPVMGHLGMTPQSVHVLGGYKVQGKGKDQATR
                     LIEDAKALEEAGAFALVLEAIPAQLAKTITHSVSIPTIGIGAGAHCDGQVLVLYDLLG
                     LFDDFVPKFVKPYAHLKADALQALRRYKEEVEQGKFPGDSESYH"
     gene            complement(2591..3481)
                     /locus_tag="VLL06_09475"
     CDS             complement(2591..3481)
                     /locus_tag="VLL06_09475"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HSS31234.1"
                     /translation="MSREPRRLREVLDQGQFAVTIEYNPPKGTNISGVLENARQLVGR
                     VHGVNVTDNTAAIVRAGSLPVCRLLYELGHDPVMQMTCRDRNRIAMQSDLMGASMLGI
                     QNILCLTGDYPTVGDHKEAKPVYDLDSVQVMQLVQGLNNGRDFVGNKLDGATQFTIGA
                     AVTPEADPLGPMLAKFEVKVKAGAQFFQTQAIYHPEQFASFMQAVRPFKVKVLAGILV
                     LRNAKMAEFMNANIPGVSVPQEMIDELRAAGPERAEDVGVDIAVRTIKAVRPHCDGVH
                     VMAIKATHRLAEIITKAEVG"
     gene            complement(3605..>4360)
                     /gene="folD"
                     /locus_tag="VLL06_09480"
     CDS             complement(3605..>4360)
                     /gene="folD"
                     /locus_tag="VLL06_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HSS31235.1"
                     /translation="LATILVGDDPASHLYVKSKQKACDAAGIYIDDHKLPASTTQAEL
                     LALIEKENADPKIHGILVQLPLPKHIDSKVVLEAVSPDKDADGFHPYNFGRLVEGHPV
                     FEACTPKGVIKMIESTGVSIEGKRAVVLGRSNIVGKPLALMLLQRNATVTICHSKTKD
                     LPAVCREAELLLVAIGKAKFVTADMVRDGAVVIDVGTNRLPDGKVVGDVDFEPVSHKA
                     GWISPVPGGVGPMTIAMLLDNTVESAKRIAKML"
BASE COUNT          908 a         1348 c         1136 g          968 t
ORIGIN      
        1 gtcgaagtcc tgcgtcgagc tgaaatccct caagatgtac atgctcgcct accgctcgct
       61 cggcatcttc tacgaaaacg ccgtcaaccg cattctgcat gatgtcgtaa aagcctgccg
      121 ccctgtatgg gccaaggtca ccggcgagtt tgcggcccgt ggcggacttc gaagcctcat
      181 tgagacgaag tacccgtgag tcccatccgc cattcgtgcc cgtgcgtctg agccactcat
      241 ctttatggct acctacgcga tcggggatgt gcagggatgc aatgaccccc tgcaacgcct
      301 agtcgaacag attcaatttg accccgtatc cgaccgcctc tggttcgtcg gcgatctcgt
      361 gaaccgtgga cctgactcac tcaaggttct ccggtacatt aaaagtcttg gaacatcggc
      421 agtcgtagtc cttggcaatc acgacctctt cttgctcgcc gtagccgacg gcattgtgac
      481 acccagcgcg caggacaccc tccaaccaat ccttgacgcc ccggatcgtg acgagttgct
      541 aggctgggta cgccagcaac gcctcctcta ccacgaatcc tccttcgttg tgattcacgc
      601 aggactgctg ccgcagtggt ccgtgcgtga ggctgaggat ctggctcgtg aagtggagac
      661 tgggctacgg agtccctact acaaagacgt gcttcgagcg ctctatccca gcaaacactt
      721 gcaatggtct tcggccctaa ccggcccgat cagacttgcc acaatcatca aagttctcac
      781 gcgactccgg gcttgttcgg ttgatggcct gatggagtcg tcatattccg ggccaccggc
      841 tcacattcct gccggctttg ttccatggtt ccgaatcgaa aaccgcgaac gacgagagac
      901 aaccattatt tgcggccact gggcctctct gggtctccat tgcgaaaaga atgtattggc
      961 catcgacagc ggatgtgtat gggggcggca gctcacagcc atacgattgg aggatcgaaa
     1021 ggtatttcaa gtcgactgca accacatcga gcgtgacggt gactgagccg gaaccgcctg
     1081 atccgcattg gcctcgttac tcaacccgtc cgttcccttc ataccgcttc gtgcctggaa
     1141 ggaccccaca tccacgccgt gatccaagcg gacattccta tggtctgtcg gagccgaaac
     1201 caacaccgtt ctcagttgac caatggcaca cctcggagga ctatctgtat ggaattgacc
     1261 tctataattt tgcctactgg tgggaatgtc acgaggtctt cgagggactg tggcacgcgg
     1321 tgggacacca cagcgagcag ggaaacttct ttcaagccct catccaagtc gccgctgcca
     1381 atctcaagca gttcctcggc aacgaacaag cggcgcagaa actcgcacgt agcgggctcg
     1441 cacgtcttca gaatctgccc caactttata tgggaatcga cgtcatcgct ctttctgaag
     1501 cgtgtagggc tatttccaca ccgcttgcga attctacgcc ctatcaaatc cgcctctcgc
     1561 ttgaaaccca ttcctcgccg gtgaacttga agagctagtt gcggatgtcg actgccattt
     1621 cgtaatagtc aggctgctca aaaaggtcat ccatctcacc ctccccgtcc cacgcgcgcc
     1681 gagacgcgcg tcttacccat ggcaaggccg caggcgagaa agaaccggag gcgtaccctc
     1741 tggggtacgt tgaggattct ttcgacccga gaacgaagtt gggggccttt ttcagcagcc
     1801 tgctagtggt aggattcgga gtcgcccgga aacttgccct gctcaacctc ttccttgtag
     1861 cgtcggagcg cttggagcgc atcggccttg aggtgagcgt acggcttcac gaatttcggc
     1921 acaaagtcat cgaagagacc gagaagatca tagaggacca gcacctgacc gtcgcagtga
     1981 gcaccggcgc cgatgccgat ggtaggaatt gaaacggaat gggtgatggt cttggccagt
     2041 tgcgcgggaa tggcttcgag taccagcgca aacgccccgg cttcctcaag agccttcgca
     2101 tcctcgatca acctggttgc ctgatccttt cctttccctt gaaccttata tccaccaaga
     2161 acgtgaaccg actgtggggt cataccgaga tgccccatca ctggaatgcc gatgttggtc
     2221 attgccacca cttgatcaac cacggctgcg ccaccttcca atttgactgc gtgagcccca
     2281 gcctgtagaa atcgtccagc attccgacgt gcgtcttcct gactggcttg ataggacatg
     2341 aagggcatat cgccgatgac gagtgcttgc tgggccgccc cggctacgag tttcgtatgg
     2401 tagagcatgt cgtccatcgt cacggaaagc gtattggctt tcccctgcac aaccacaccc
     2461 agtgagtccc cgaccagaat cgtctgaatc ccggcctgtt cgacaatacg tgtgaacagc
     2521 gcgtcgtagg ccgtgacgac aatgagcttc ttcttgtcac gcttgtgccg ctgaaactcg
     2581 ggaatcgtca tcaacccact tccgctttgg tgatgatctc agccaatcgg tgcgtggcct
     2641 tgatcgccat aacatgaacc ccgtcgcaat gcggccgcac cgccttgatc gttcgcaccg
     2701 cgatgtccac ccccacgtct tctgcacgct ctgggccggc agccctgagt tcgtcgatca
     2761 tctcctgggg gaccgacaca ccgggaatat tggcattcat gaattccgcc atcttcgcat
     2821 tccggaggac aagaatgccc gctaacactt tcaccttgaa ggggcgcacc gcctgcatga
     2881 aggacgcaaa ctgttccgga tgataaatcg cctgcgtctg gaaaaactgg gcacccgctt
     2941 ttaccttcac ttcaaacttg gcgagcatgg ggcccagcgg atctgcctcc ggagtcaccg
     3001 cagccccaat ggtaaactgc gtcgcaccat ctaacttgtt ccccacaaaa tcacgcccat
     3061 tgttgagtcc ttgcacgagc tgcatcacct ggacagaatc cagatcatag accggctttg
     3121 cctctttgtg atctccgacg gtcggatagt ccccggtgag gcagagaatg ttttggatgc
     3181 cgagcatgga agcgcccatg aggtccgact gcatcgcaat acgattccga tcacggcagg
     3241 tcatctgcat gaccggatca tggccgagtt cgtagaggag tcggcagaca gggagagacc
     3301 cggcccgcac aatggcggcc gtgttgtccg tcacattgac cccgtgcacg cgccccacca
     3361 actgcctggc attctccagc acaccggaaa tgttggtgcc tttgggagga ttgtactcaa
     3421 tcgtgacagc aaactgtcct tgatcaagca cttctcgtaa tcgcctcggc tcccgactca
     3481 tcttctttcc gtaccccctt acacttcacc cgctgttgta ggaggattgg ggctccctac
     3541 tgcgcgcatt gaccgagcac attctgatcg tgtgcgggca gcgagcaatg gacccctcat
     3601 ccttctacaa catcttcgcg attctcttcg cagactccac cgtgttgtcc aacagcatcg
     3661 caatcgtcat cggcccgaca ccgcccggca cggggctgat ccagcctgcc ttgtgactga
     3721 caggttcaaa atcaacgtcg ccgacgacct tcccgtcggg gagccggttc gtcccgacgt
     3781 cgatcacgac tgccccgtcg cgtaccatgt cggccgtgac aaacttggct ttcccaatcg
     3841 cgaccaacag caactcggct tcgcgacaga ccgccggcaa gtccttcgtc ttggagtgac
     3901 aaatcgtgac cgtggcgttg cgctggagca acatgagcgc caacggcttg ccgacgatgt
     3961 tactccgtcc caatacgacg gcacgcttgc cttcaatact cacgccggtg gattcgatca
     4021 tcttaatgac gcccttcggc gtgcaggcct cgaacacggg atgcccctcg acaagccgac
     4081 cgaaattgta ggggtgaaac ccgtctgcat ccttgtctgg cgacactgct tcgagcacaa
     4141 ccttgctgtc gatatgcttt gggagcggca actgaaccag tatcccgtgg atcttggggt
     4201 cggcgttttc tttttcaatc aaggccaaca actctgcttg ggttgtgctc gccggcaatt
     4261 tgtgatcatc gatgtagatg cccgccgcat cgcaggcttt ctgcttgctc ttcacataaa
     4321 gatgcgaggc gggatcgtcg ccgaccagga tggttgccag
//
LOCUS       DATIPF010000377         5643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_692167, whole genome shotgun sequence.
ACCESSION   DATIPF010000377 DATIPF010000000
VERSION     DATIPF010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692167"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VLL06_09485"
     CDS             complement(<1..519)
                     /locus_tag="VLL06_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSS31236.1"
                     /translation="MTKQGREIDVGHYRIEQEPFYAEVRGEIGLFTIAANSKMPVMLK
                     GPTGCGKTRFVQHMAYRLGRPLITVACHEDLTASDLVGRYLLKGQETVWVDGPLTLGV
                     KHGAIVYLDEIVEARKDTTVIIHPLSDDRRFLPIEKKGQILEAADDFLLVISYNPGYQ
                     SVLKDLKQSTKQR"
     gene            complement(937..4044)
                     /locus_tag="VLL06_09490"
     CDS             complement(937..4044)
                     /locus_tag="VLL06_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSS31237.1"
                     /translation="MKTSRALSFLDLRRYRWLPHLLVVMTVFALLAGIVLIRFVEQRF
                     VGVTGGELTLAAVEVAEKLDRMLFERRGDVLMMARAFSSRTSDPQYLSEYLKWMQKEY
                     SPVYLSLAVLDVQGTVMAATDTSLVGRNYDRAVSFATVRATRQLHVTDVAVQETGESG
                     VEAITFTAPILSPQGAFLGVVTLQVGIPFLEEVTTRTIRSLEARPGATGLVEYQMLTR
                     QGRAFVDSDLLHKGAINLKELGLPSALLSEAGVPGFIEEQHLQRHVQVVTGYAQTKGF
                     GEFAGLGWSVLVRMERQEILAPIHDFLWKVGIIGGAVWFPMLALLFWATARLRAEHRQ
                     AQQESAWAKAAEAALLQSQERNRAIVDTALDGVITIDSTGIVTDWNAQATAIFGWSRE
                     EALGRALSETIITEGDRQAHEQGIREFLRTGTGPILNRRIEIVAQHRDGRKIPVELAV
                     SPAKIGDAYIFSAFIRDITDRRAAERRLASQYAVTRVLSEATTMEEAVPKIIQAIGEN
                     LEWDLGVFWRVDKATGVLRCFDQWKVPFVQADPFTQDTWQRVFAPDEGFPGRIWASDK
                     PAWITDVAIDSHFSRGVQARQVGFHGAFGWPIRVGSAIDGVIEFFSRQVRQPDDDLLK
                     MVEDIGLKIGQFGERARTEGVLRETEAQLRQAQKMEAVGRLAGGMAHDFNNLLTVIRG
                     YSELLLGRLGPTDDMRKDMEEVKKAADRASGLTRQLLAFSRRQFIAAKVLDLNALVAN
                     MDGMLRRLIGEDIVELSGELDTSLGAIKADPGQIEQVIMNLVVNARDAMPKGGRLTIE
                     TRNVTIGEEVRLDAVGVAPGSYVLLTVRDNGHGMDAETRSHLFEPFFTTKEKGKGTGL
                     GLSTVYGIVKQSGGSIIVESTPGRGTTFRIYFPRVEQEIPGPTGAADTIDPVRGRETI
                     LLIEDEPAVRGLVHETLRLHGYTVLAARHGIEALLASAKYVGPIHLILTDVVMPQMSG
                     PEVAEKILTVRPGIKVLYMSGYPDHPVFDQGSVRRESGFLPKPFSPHVLVQKVREVLD
                     SVKAA"
     gene            complement(4041..5510)
                     /locus_tag="VLL06_09495"
     CDS             complement(4041..5510)
                     /locus_tag="VLL06_09495"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HSS31238.1"
                     /translation="MTRPIVLPQLVPSRTCLQCDVCCRFPDPDSTLRPYFTESEITRA
                     LAGGVEGTCFPNHRGSQVLLVPAPQGDGYLCPAFDASTSTCRIYEQRPLDCQLYPLAL
                     MWNEPHDQILLGWDTKCPFMREEIPEEIQRHADRVMALLDQPDIRDHVVAHPRLIGRF
                     QQDVVVLDRLPRLTVAVGSQWGSAPLYRFTLDDFPRMTAALDRSGWCRDQSLAAYSPV
                     YHYMWSGVLGYWWMELQGVFCLIAQSPDGWFMPLPPIGAGSIEAALSQAMKQMRRWNG
                     DSPVSRVENVPPHLAPELERLGYRVTPKEPDYLYRATDLAALSGDRYKAQRALCNRFE
                     REQSCEVGSYQVGDRQDCRALLRDWSRQKQGEGLDPFSAMLLADAGPAHEVIWSQASA
                     LRVTGKVVRIHGRICAYTFGYWLTSATFCVLLEVTDRSLPGLAQYLFRETCRTAVTEG
                     AEYVNAMDDAGLPGLRASKQAYHPAMLISNFVASPARES"
BASE COUNT         1233 a         1775 c         1487 g         1148 t
ORIGIN      
        1 gcgctgcttc gtgctctgct tcaaatcttt gagcacactc tgatacccgg gattatagga
       61 aatcaccagg aggaagtcgt cggccgcttc gagaatctgg cctttctttt caatcggcag
      121 gaagcggcga tcgtcgctca agggatggat gatcaccgtc gtatccttac gcgcctcgac
      181 gatttcgtcc aaatagacga tcgcgccatg cttgacgccg agtgtcagcg gcccatcgac
      241 ccacaccgtc tcttggcctt tgagcaaata ccgccccact aaatcggacg cggtgagatc
      301 ctcatgacag gccaccgtga tcaaggggcg gccaagccgg taggccatat gctggacgaa
      361 tcgggtcttg ccgcacccag ttggcccctt gagcatcacc ggcatcttac tgttcgccgc
      421 gatcgtgaac aaaccgatct caccgcggac ctctgcatag aatggttcct gctcgatccg
      481 atagtgcccg acgtcgattt cgcggccttg ctttgtcatc tcacgctcac ccggtcagcg
      541 gtgtcctttg cttcacgcct gcttcacgtc ttgtgaaggg cacgataaac ggaaccgagg
      601 ctgaagatca agcgatctgg ttagaacaac cgtgtgcggc cgagcaggct ccggaaaaac
      661 tatgttggct cgtaaagtct tcagtggtcc gcacgtgtga aacaccggga caactctccc
      721 gcaggatgct caaaaaggcc gtccagctag gccgcaggcg agtcgaaacc ggaggcgtag
      781 ttcttctcac ccgcccaccc caagctgcca agccagcttg tcctccgagg gggtacgttg
      841 aggatttcga taaaccgaga acgacgcatg ggaacaggcg cgtcttggcg cgctggggtt
      901 gggcgggtga gaagaacgac tttttcagca tcctgcttag gcggccttca cactgtccaa
      961 gacttcgcgc actttttgca ccaacacatg aggtgagaag ggcttgggca ggaatccgct
     1021 ctcgcgccgc acactgcctt gatcaaatac cggatggtcc gggtagcccg acatgtatag
     1081 cactttgatc cctggccgca cggtcagaat cttctccgca acttcaggcc cactcatctg
     1141 tggcatgacg acatccgtca atatcagatg gataggcccc acgtattttg cgctggcgag
     1201 aagggcttcg atgccatggc gcgccgccag gaccgtataa ccatggagcc gcaacgtctc
     1261 gtgtaccaga ccacgaaccg ccggctcgtc ctcgatcaag aggatcgttt cgcggccgcg
     1321 taccggatcg atggtgtcag ccgcccctgt cggccctggt atctcttgtt cgacccgtgg
     1381 gaaatagatc cggaacgtag taccccggcc tggtgtactc tcgacgatga tacttccccc
     1441 gctctgcttg acgatgccat agacggtgga gagccccaat cccgtccctt tccctttctc
     1501 cttcgtcgtg aagaacggtt cgaacagatg cgaccgggtc tcagcatcca tcccatggcc
     1561 gttatcacga accgtcagca acacatacga ccccggcgcc acccccaccg catccaaccg
     1621 cacctcttcc ccaatcgtca cgttccgggt ttcgatcgtc agacggccac ctttgggcat
     1681 cgcatcacgt gcattgacca ccaggttcat gatgacctgc tcgatctgtc cgggatccgc
     1741 cttgatcgcc ccaagcgacg tatcgagttc gccactgagt tcgacgatat cttcaccgat
     1801 gagacgccgt aacatgccgt ccatattggc gacgagcgca ttgagatcga gaactttcgc
     1861 cgcgatgaac tgacggcggc tgaacgccag caattgacgg gtcaacccgg aggcccggtc
     1921 cgccgccttc ttgacctcct ccatgtcctt ccgcatgtca tcagtgggcc ccagacgccc
     1981 cagaagcaac tcgctgtacc cacgaatgac cgtgaggaga ttattgaaat cgtgcgccat
     2041 gccgccggcc agccgtccca ccgcctccat cttctgcgcc tgccgcaatt gcgcctcggt
     2101 ttcccgtaac accccttctg ttcgagcccg ctcgccgaat tggccgatct tgagaccgat
     2161 gtcctccacc atcttcaaca gatcgtcatc aggctgccgc acctggcggc tgaagaattc
     2221 gatgacgcca tcgatcgcac ttccaacacg aataggccat ccaaacgccc catgaaatcc
     2281 aacctgccga gcctgcactc cacgagagaa gtgactatct atggcaacgt ctgtgatcca
     2341 ggccggcttg tcactggccc aaatacgtcc agggaacccc tcgtcgggtg caaacacacg
     2401 ttgccaggtg tcctgggtaa acggatcggc ctgcacgaac ggcaccttcc actgatcaaa
     2461 acagcgcaac acaccggtcg ccttatccac ccgccaaaac acgcccagat cccattccag
     2521 gttctccccg atggcctgaa tgattttcgg aacagcctct tccatggtcg tcgcttcgga
     2581 gagaactcgg gtcaccgcat actgagaggc gagacgtcgt tcggccgcgc ggcggtcagt
     2641 gatatctctg ataaaggcgc tgaagatgta tgcatccccg atttttgccg gtgacaccgc
     2701 aagctcaacg gggatctttc ggccatcccg gtgttgggcg acaatttcaa tccggcggtt
     2761 caagatcggg ccagtccctg ttcggagaaa ttcacggatg ccctgttcat gcgcctgacg
     2821 atccccttca gtaatgatgg tctcagagag ggctcgaccc aacgcttctt cacgggacca
     2881 cccaaagatc gccgtcgcct gtgcattcca gtcggtcaca atcccggttg aatcgatagt
     2941 gatcactccg tcaagcgccg tatcgacgat ggcccgattc cgctcctgac tttggagcaa
     3001 cgccgcttca gcggctttcg cccaggcact ttcctgttgt gcttgccgat gttcggcgcg
     3061 caagcgtgct gtggcccaaa acaacagggc cagcatggga aaccagactg ctccgcctat
     3121 aatcccgact ttccacaaga agtcatgaat cggcgccagg atttcctgtc gctccattcg
     3181 taccaggacc gaccatccca gcccggcaaa ttctccaaaa cctttagtct gggcataccc
     3241 agtcaccacc tggacatgcc gctgaagatg ctgttcctcg ataaaccctg gtacgccggc
     3301 ttcactcaac agggcagaag gaaggccaag ttctttgaga ttgatggccc ccttgtgcag
     3361 caggtccgag tcgacaaacg ccctgccttg ccgcgtcaac atttggtatt cgaccagccc
     3421 ggttgctccc ggtcgagctt caagcgagcg aatcgtccta gtggtcactt cttccaaaaa
     3481 ggggatcccc acctgcaacg tcaccacacc caggaacgcg ccttgagggc tcagaatcgg
     3541 agcggtaaac gtaatcgcct cgacaccact ctccccggtt tcctgcactg cgacgtctgt
     3601 cacatgaagt tgtcgggtcg cacgcacagt ggcaaacgag acggcacggt catagttgcg
     3661 tcctaccagc gaagtgtctg tcgctgccat gactgtaccc tgaacgtcca aaaccgctaa
     3721 cgacaggtag accggggaat actctttctg catccacttc aaatactcgg acagatattg
     3781 tggatcggag gttcgcgacg agaacgctcg ggccatcatg aggacatcgc ctcggcgttc
     3841 gaacagcata cggtcgagtt tctcggccac ttctacggcg gccagcgtga gttctcctcc
     3901 cgtcacccct acaaaccgct gttcgacgaa ccgaatcagg acgataccgg cgaggagcgc
     3961 gaatacggtc atgacaacaa ggaggtgggg caaccagcga tagcggcgaa gatcgagaaa
     4021 tgataatgcc cttgaggtct tcatgattca cgcgccgggg aggcaacgaa gttggatatc
     4081 agcatggcag gatgataggc ctgtttcgat gcgcgcaatc cgggaagacc tgcatcatcc
     4141 atcgcattca cgtattcagc cccctctgtc acagccgtcc ggcaggtctc ccggaagaga
     4201 tactgcgcca gacctggaag ggaccgatcc gtgacttcta agaggacaca gaatgtcgcg
     4261 ctcgtcagcc agtagccaaa ggtataggcg cagatccgac cgtgaatcct cacgaccttt
     4321 cccgtcactc gaagggccga cgcctgcgac cagattactt cgtgagccgg acccgcatca
     4381 gccagcaaca tcgcagagaa cggatcaagc ccttcccctt gcttctgccg tgaccaatcc
     4441 cttagcaggg cacgacagtc ctgacgatcg ccaacctgat aggagcccac ctcacacgac
     4501 tgctctcgtt cgaatcgatt gcacaatgcg cgttgagctt tataccgatc gcctgataag
     4561 gccgcgaggt ccgtcgcgcg atacagatag tccggttctt tgggcgtcac ccgatatccc
     4621 agccgttcta actctggtgc caaatgcggt ggcacgtttt caacccggct gactggagaa
     4681 tcaccgttcc aacgtctcat ctgcttcatc gcctgagaca gggccgcctc gatcgagccg
     4741 gctccgatcg gaggcagcgg cataaaccag ccgtccggcg actgtgcgat aagacaaaag
     4801 actccctgaa gctccatcca ccaataaccc agtactccac tccacatata gtgatagact
     4861 ggcgaatagg ctgcgagcga ctgatcgcgg caccacccag accgatccaa tgctgccgtc
     4921 atgcgtggga agtcatccaa tgtgaaccga tacagcgggg cagatcccca ctgactaccc
     4981 acggccacgg tgaggcgcgg aaggcggtcg agcaccacca cgtcctgttg aaaccgtccg
     5041 atgaggcgag gatgcgcgac gacgtgatca cggatgtccg gttgatcgag caaggccatc
     5101 acccgatcgg catgacgctg tatctcctcc ggaatttcct ctcgcataaa cgggcatttt
     5161 gtgtcccacc ccaacaagat ctggtcgtgt ggttcattcc acatcagcgc cagtggatag
     5221 agctgacaat caagcggacg ctgctcatag atgcggcagg ttgaagtcga tgcatcgaat
     5281 gccgggcaga gatatccgtc tccttgggga gcgggaacca ggagcacctg tgacccccga
     5341 tgattgggaa agcacgttcc ctcgacaccc ccggccagag ccctggtaat ttcgctctcg
     5401 gtaaagtagg gacggagggt gctgtcggga tctgggaacc gacagcagac atcgcactgg
     5461 agacaggttc ggctcggcac gagttgtggc agcacgatcg gtcgtgtcac gaaagcgccc
     5521 accagcttat cgtagataag gacattgaag gattccaatt gatgtgcggt tcatactagc
     5581 accattcaca ggggtgccca aagggatgtc acttttttca ccggtgcctc gtccctcaca
     5641 atc
//
LOCUS       DATIPF010000378         1637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_428629, whole genome shotgun sequence.
ACCESSION   DATIPF010000378 DATIPF010000000
VERSION     DATIPF010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428629"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VLL06_09500"
     CDS             <1..286
                     /locus_tag="VLL06_09500"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSS31239.1"
                     /translation="RAALEPFPGAAAYLVAKAALVSLIRVLALELGGAGVTVNGILPG
                     TIDTPANRESMPQADRSTWVKPESIAQVLVWLMSDDTAAINGALIPVGPS"
     gene            307..561
                     /locus_tag="VLL06_09505"
     CDS             307..561
                     /locus_tag="VLL06_09505"
                     /inference="COORDINATES: protein motif:HMM:NF022397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HSS31240.1"
                     /translation="MSDHGEDQSVLNHIQKLVTEEHRLYALGAGESSPGTDREKLAKI
                     QVELDQCWDLLRQRRGLRDAGQDPSKAQVRPPGVVENYAQ"
     gene            complement(577..759)
                     /locus_tag="VLL06_09510"
     CDS             complement(577..759)
                     /locus_tag="VLL06_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31241.1"
                     /translation="MTITTVSLRRVIFKATARPILLMSDESKSTVEGRKRLTSSFLFK
                     TSALAAQAAASAESPL"
     gene            796..1170
                     /locus_tag="VLL06_09515"
     CDS             796..1170
                     /locus_tag="VLL06_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSS31242.1"
                     /translation="MKIPSAAKAKKYFQDKMSFTTGPVELERWMKQGQPVNVVDVRAA
                     EDYAEGHIPAASNLPKDQWDNPKQLKAILRKDKINVLYCYSQVCHLAATAAVEFTKKG
                     YPVMELDGGWRWWTTDGFDIEK"
     gene            1329..>1637
                     /locus_tag="VLL06_09520"
     CDS             1329..>1637
                     /locus_tag="VLL06_09520"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HSS31243.1"
                     /translation="MTARTHHTGLAVIAVFKVVKGVLLLLVGLGLLKLVHADIATLFS
                     QLIEGLHLNADSRLIHALVLKVDALQPNSVLLAGLVSLGYAGMLLVEGVGLWLEFTWA
                     A"
BASE COUNT          369 a          421 c          514 g          333 t
ORIGIN      
        1 gcgtgctgcg ctggaaccgt ttccaggagc tgcggcctat ctagtagcga aggcggcact
       61 cgtctcgttg atacgagtcc tggcgctcga gttgggaggg gctggggtca cggtgaacgg
      121 tattctccct ggaacgatcg acacgccggc aaaccgggag agtatgcctc aagccgaccg
      181 atccacctgg gtaaaaccgg agtctatcgc gcaggtgctc gtgtggctca tgtccgatga
      241 cacggcggcg atcaacgggg cactcatccc tgttggacca tcgtaagaag tagaaaggag
      301 gcatctatga gcgatcacgg cgaagatcag tcggtactga accatatcca gaagttggtg
      361 acagaggagc atcggttata tgcattaggg gcgggagagt catcgcccgg cacagaccgt
      421 gagaagctcg cgaaaatcca ggtcgaactg gatcaatgct gggacctgct gcggcagcgc
      481 cgaggcttgc gtgacgccgg acaggacccc agcaaagcac aggttcgtcc cccgggcgtc
      541 gttgagaact atgcacagta gcgagtcgat gtgagcctat aaggggctct ctgcgctggc
      601 ggccgcctgg gccgccagcg cagaagtctt gaacaaaaac gaagaggtca gccgtttgcg
      661 gccttcgacg gtggacttag actcgtcgct cataagaagg atggggcgag cggtggcctt
      721 gaagataacc cgtcgaagcg agacggttgt gatcgtcatc tggccagaac agagaggcga
      781 accatcgtga caaagatgaa gattcccagc gcagcgaagg ccaaaaagta ctttcaggac
      841 aagatgtcct tcacgaccgg ccccgttgag ctggagcgat ggatgaagca gggtcaaccg
      901 gtgaacgtcg tggacgtgcg ggccgcggaa gattatgccg agggccatat cccggccgcg
      961 agcaatttac cgaaagacca atgggataat cccaagcaac tcaaagctat cttgcgtaag
     1021 gacaagatca acgtcctcta ttgctattca caggtgtgcc accttgctgc aacggcggca
     1081 gtcgaattta cgaaaaaggg ctacccggtg atggagttgg acggcggctg gcgctggtgg
     1141 acaaccgatg ggttcgacat cgagaagtaa agctctccgc atgccgtcat gaaactgtca
     1201 gttccgacca tgcacaaggt ttgaggatca ggccttataa caatatatat ggtctcaatc
     1261 cgttcgtcac agccctgcaa gtcacttacc ggttcaatcc tcgccgtgtg tgatagtctc
     1321 tcgaattgat gacggcacgc acacaccata ccggcctggc cgtgattgcg gtgttcaagg
     1381 tggtgaaagg ggtgctgttg cttctcgtcg gcttggggct cctcaaattg gtccatgccg
     1441 acattgccac gctgttttcc cagctgatcg aagggctgca tctgaatgcc gactcccgac
     1501 tcattcacgc cttggtgctg aaagtggatg cgctgcagcc gaatagtgtg ctgttggcgg
     1561 ggcttgtcag tctgggctat gcggggatgt tattggtgga aggggtcgga ctgtggctcg
     1621 agttcacctg ggctgcg
//
LOCUS       DATIPF010000379         3084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_958337, whole genome shotgun sequence.
ACCESSION   DATIPF010000379 DATIPF010000000
VERSION     DATIPF010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958337"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /gene="lepA"
                     /locus_tag="VLL06_09525"
     CDS             complement(<1..1386)
                     /gene="lepA"
                     /locus_tag="VLL06_09525"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HSS31244.1"
                     /translation="MQSLIRNFSIIAHIDHGKSTLADRFLEATGAVTARESKDQILDA
                     MDLERERGITIKAHAVAVRYQAKDGKTYALHLIDTPGHVDFTYEVSRSLAACEGALLL
                     VDATQGVQAQTIANVNLAMANHLTIIPVINKIDLASADPEGTKQSISDILMLDATDAL
                     PISAKEGKGVPEVLEAIIARIPPPAGDPKAPLKALIFDSWFDNYQGVIVLTRVVDGSL
                     RPGMKIKVMSNDRMFEVMEVGQFTPKRTKRTELLTGEVGYLCANMREVADVKIGDTLT
                     DAVSPTGAPFPGYKEVKPLVFCVLYPTDTGRYEDLRDALVKLRLNDSSFVYEPETSLA
                     LGFGFRCGFLGLLHMEIIQERLEREYNLTLLTTAPTVVYRVLTTKGEVLEVNNPTQLP
                     PPSSIDAFEEPFILASVITPERYMGAILQLCQERRGIQRSMHFLDPTRVVISYELPLN
                     EVILDFYDKLKS"
     gene            1598..2965
                     /gene="bioA"
                     /locus_tag="VLL06_09530"
     CDS             1598..2965
                     /gene="bioA"
                     /locus_tag="VLL06_09530"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HSS31245.1"
                     /translation="MAQRPSTRQLIDWDHQYLWHPFTQMQEWEQEEPLIIERGKGPYL
                     IDTEGKRFLDGTSSIWVNLHGHRHPTLDRAIKKQLDNIAHSTLLGLANPPAIQLARAL
                     IHIAPKGLKRVFYSDNGSTAVEIALKMAVQYWQQRHPEAGSKNSFLHLKMAYHGDTIG
                     AVSVGNIAVFHARFKSLLFPTIEANPPYCYRCPLGLTYPSCEMACIDPIEQILKERHR
                     ELAGFIIEPLVQAAAGMLPSPPGYLTRIRELCSRYNILLIADEVATGFGRTGKMFACQ
                     HEGVTPDLMAISKGLTGGYMPLAATLTTDEIYQAFLGKYEDFKTFFHGHSYTGNPLGC
                     AVALANLDVFRKDKTLTRLQPKIKTLARLLQPLWQLPHVGDIRQRGFIAAIELVTDKT
                     TRTPYPLEARIGHRVCTIARERGLLLRPLGHVILLIPPLTTTHDVLKRMVDILRESIE
                     ALPTN"
BASE COUNT          695 a          928 c          783 g          678 t
ORIGIN      
        1 cgacttgagc ttatcgtaaa aatcgagaat gacctcgttg agtggcagtt cgtaactgat
       61 caccacccgt gtcggatcga gaaaatgcat gctccgttga atcccccggc gctcttgaca
      121 gagctggagg atcgccccca tgtaccgttc gggcgtaatg accgacgcca gaataaatgg
      181 ctcctcaaag gcgtcgatac tgctgggggg cggaagctgc gtcgggttat tgacctccag
      241 cacctcgccc tttgtcgtca gcacgcgata gacaacggtc ggtgcggtgg tgagcagcgt
      301 caggttatac tcacgctcga gccgttcctg gatgatttcc atgtgcagca ggcccaaaaa
      361 cccgcatcga aacccgaacc ccaatgcgag cgacgtctcc ggttcgtaga cgaaggagga
      421 gtcgttcaac cgcaatttca ccagcgcatc gcgcagatct tcgtaccggc cggtgtcggt
      481 gggatagagc acacagaaca ccaggggctt cacctctttg tacccgggaa atggcgcacc
      541 cgtcggcgac accgcatccg tgagcgtatc gccgattttg acgtcggcga cctctctcat
      601 atttgcacac agatacccga cctcgccggt cagcagctca gtccgtttgg tccgtttcgg
      661 ggtaaattgg ccgacttcca tcacttcaaa catccggtcg ttcgacatga ctttaatttt
      721 catccccggc cgcaacgatc catccacgac tctcgtcagc acaatcacgc cctgataatt
      781 atcaaaccag gaatcaaaga tgagtgcctt gagcggtgcc ttgggatcac cagccggtgg
      841 agggatcctg gcgatgatcg cctccagcac ctccggcaca ccctttccct ctttcgcgct
      901 gatcggcagg gcatcggtcg cgtccagcat caggatgtcg gaaatcgatt gcttggtgcc
      961 ttctggatca gcgctcgcaa gatcgatctt attgatgacc gggataatcg tgaggtgatt
     1021 ggccatcgcc agattcacat tggcaatcgt ctgagcctgc accccctgtg tggcatcgac
     1081 gagcaacaat gcgccttcgc aggccgccaa actgcgtgag acctcatagg taaagtcgac
     1141 gtgacccggc gtatcgatca aatgcaaggc atacgtcttc ccatccttgg cctgataccg
     1201 aaccgcgacc gcgtgggcct tgatcgtgat cccgcgttca cgctccagat ccatcgcgtc
     1261 gagaatctgg tctttcgatt ctcgggccgt gacagcgccc gtggcttcca ggaaccggtc
     1321 agcgagggtt gatttgccgt gatcgatatg ggcaataatc gagaaattgc gtatgaggct
     1381 ttgcaaattc taactctttt cccaataaca tcgcgttatt atagttattg ccccccaggt
     1441 tgtcaaagca atcgccccct cgtataatcc gttccgcgcc gcaccggcaa cattcaatac
     1501 gagtgtatga ccgatcgtct atcgcgaagc taagggtcct cgtttttgat ttcgagctct
     1561 acgccgttca cgcttccact tctacgactc ctctaccatg gcacaacgac cttctactcg
     1621 acagctgatt gactgggatc accaatacct ctggcatccc ttcacccaaa tgcaggaatg
     1681 ggaacaggaa gagccgctca ttatcgagcg gggcaaagga ccctacttga tcgataccga
     1741 aggaaagagg tttctcgatg ggacctcgtc catctgggtg aatctccatg gccatcggca
     1801 tccaacgctc gaccgagcca ttaagaagca gctcgacaat attgcgcact ccacgttgct
     1861 cggactcgcc aacccaccgg cgattcagct cgcacgtgcc ttgattcaca tcgcacccaa
     1921 aggtttgaag cgggtgttct actccgacaa tggatcgaca gccgtcgaaa tcgcgctcaa
     1981 aatggcggtg caatactggc agcagcgtca ccccgaggcc ggttcaaaaa attcctttct
     2041 ccatctgaag atggcctacc atggcgatac catcggggct gtgagtgtcg gcaacatcgc
     2101 ggtcttccat gcgcgtttta agtcactctt gttccccacc atagaggcca acccacccta
     2161 ttgttatcgc tgcccgttgg ggttgaccta cccttcgtgc gagatggcct gcatcgatcc
     2221 gattgagcag atcctcaaag agcgacaccg tgaactggcc ggcttcatca tcgagccact
     2281 tgtacaagcc gccgcgggaa tgctgccctc tcccccaggc tatctgacac gaattcgtga
     2341 actgtgcagc aggtacaata tcctgttgat tgccgatgaa gttgctacag gctttgggcg
     2401 taccggaaaa atgttcgcct gccagcatga gggggtcacg ccagacttga tggcaatcag
     2461 caaaggcctg acgggtggct acatgccact cgccgcgaca ctcaccaccg acgaaatcta
     2521 ccaggccttt ctcggcaagt atgaagactt caaaaccttt ttccacggac acagctatac
     2581 cggcaatccg ctcggctgcg ccgtcgcact ggccaatctc gacgtgttca ggaaggacaa
     2641 gacgctcact cgactgcagc ccaagatcaa gacgctggcg cgactcctcc agccgctgtg
     2701 gcaactaccc catgtgggtg acattcggca gcggggattc atcgcggcca tagaactcgt
     2761 aacggataaa acgactcgga cgccctatcc gctcgaagct cgaatcggcc atcgcgtgtg
     2821 cacgatcgcc cgagagcgtg ggctgctact tcgcccatta ggacacgtca tcctgcttat
     2881 cccaccactc acaaccacgc acgatgtatt aaagcgaatg gtggacattt tgcgcgaatc
     2941 aatagaagct ttacctacaa actgagcctc cttaagacgg cactctttca tattgagaat
     3001 acagttcaaa tggggaccag atgactacgt gcgacttcct tgtcatcggc ggcggtgtga
     3061 tcggcctcag tatcgtccga gagc
//
LOCUS       DATIPF010000380         6328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_780689, whole genome shotgun sequence.
ACCESSION   DATIPF010000380 DATIPF010000000
VERSION     DATIPF010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780689"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..756
                     /locus_tag="VLL06_09535"
     CDS             118..756
                     /locus_tag="VLL06_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSS31246.1"
                     /translation="MTTMIRKTFSVPPLGCNCSIIGDPVTKQAIVVDPGGAPERILHE
                     LHHLGLTVSHILHTHAHFDHFLASGEMKKATGAMICLHQSDRELWKNLELQCRMFGVV
                     YVPVPMPDYWLKDEERLLVGQVSAVALHTPGHTPGSMSFYFPGDNVVLAGDTLFRGSI
                     GRTDLWGGDFDAIEQSIRERLYTLEDATIVVTGHGAETEIGLEKETNHIIRA"
     gene            775..1281
                     /locus_tag="VLL06_09540"
     CDS             775..1281
                     /locus_tag="VLL06_09540"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HSS31247.1"
                     /translation="MMACKRFALSLCFLVALVLPVAQASEPSPVLSEQVTITDVQVRI
                     SDHGTVVLLLAEGKAIPIFVDLTVALSIQGALDGEKPHRPLSHDLMHTMMETFGAHVT
                     QTIITLKEGTYYGALAVAFQNEVKVFDSRSSDSIALAIHFKAPILVGRDLLNSAGRVL
                     GESKTEKL"
     gene            complement(1286..1582)
                     /locus_tag="VLL06_09545"
     CDS             complement(1286..1582)
                     /locus_tag="VLL06_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31248.1"
                     /translation="MTAPALVFILLGASWGIAWAADPPCDKYPVAKQATCAAIWKALN
                     QEDGPSIAQFGLDQLKRREEGKINAEQHLGENMAFIKQSTDKRLERLRVRMEKE"
     gene            1586..2251
                     /locus_tag="VLL06_09550"
     CDS             1586..2251
                     /locus_tag="VLL06_09550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSS31249.1"
                     /translation="MSTLRINGPSSSANDSTTQLVLQTIDVYERSAKECLARWSQRRH
                     RRPPLLVEWLRCLPANARLLDLGCGGGQDAGDLGRRGYRVVGVDRTKALLSAGRRRYR
                     SLPLVHADLRDLPFHLKSFDGIWAAASLIHLPKADARRVVTDLCKLIRPGGLFAATVT
                     HGVKSHLMTDGWLPGRYLARWRKEEFARAVRRAGWNVLELKVITNRERKGRWLNLLAQ
                     KQD"
     gene            complement(2265..2684)
                     /locus_tag="VLL06_09555"
     CDS             complement(2265..2684)
                     /locus_tag="VLL06_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31250.1"
                     /translation="MKNWKIWQGGIVLYALVAGLSGCGTWMHSDKQSVTIFTNPPETA
                     VVIDNYLHLTAPGTVTLSRKGDHLAQVNREGFEPTSFKIDRTWSWWVVADIFGCLVVF
                     SPFCIMHDIDQGGFYTFDDKVYLTLNRHTASEPPVSK"
     gene            complement(2894..3883)
                     /gene="trpS"
                     /locus_tag="VLL06_09560"
     CDS             complement(2894..3883)
                     /gene="trpS"
                     /locus_tag="VLL06_09560"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HSS31251.1"
                     /translation="MTTPRKRVLSGMQPSGLLHLGNYLGALEKWKSLQNEFECYFFVA
                     DWHALSTNYADTSRIREFVRELLIDWLAAGIDPAKATVFVQSRIPEHAVLHLLLSMMI
                     PIAWLERNPTYKEKQEEIKERDLTTYGFLGYPVLQAADILIYKPDFVPVGKDQLPHLE
                     LTRELARRFNSLYKPVFPEPMEQLTRFPKVLGTDGRKMSKSYNNTINLSDPEPVVRQK
                     LKTMVTDPARVRRHDPGNPDICPVYDFHKIFSLPMIQEQINTECRTAAIGCIDCKKLV
                     ADRIVERMTPMWDARARLSKDPAHLNDIVTEGSRRAGEVAKATLHDVNEAMNI"
     gene            complement(3880..4623)
                     /locus_tag="VLL06_09565"
     CDS             complement(3880..4623)
                     /locus_tag="VLL06_09565"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSS31252.1"
                     /translation="MNSLPHIIHTISYMALPLLFAMVLHEYAHGWVAYRCGDSTAKLQ
                     GRLTVNPLAHIDPFGTIILPLLCLLMPGGFLLGWAKPVPIDPRAMHRPRRDMALVAAA
                     GPAMNLALAIGSALLLAILLSIDPTIRDYLLNSEAASSPGTLQQMFLQPIAVMAAYSV
                     LINVLLMLFNLLPLPPLDGGRILTSLLPYTAAMALSRVEPYGMFILMGLIILDPQIHV
                     IHTIAGTLTYSLTNSILSTAVSLGAGNTP"
     gene            complement(4636..>6328)
                     /locus_tag="VLL06_09570"
     CDS             complement(4636..>6328)
                     /locus_tag="VLL06_09570"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HSS31253.1"
                     /translation="GAALHRAKQDADALKYLRQLQTEFPNSEVTDRGKLLQARVHAAM
                     GNLDLALPILTGLRNLSKDDGTKREALQLSGEFYAQKNDTTRAIQAWLDELALGTEDQ
                     AEDVRTRIRELASETSDKKALEQIRSLFPRSFPGDLASLRLIDLYIGRGEEHQAIRQI
                     QQFLVNFPIHPQAAKTSELLVTLQAKLHSNQFFIASVLPLSGKLAPFANEVLSGIQLA
                     VEANRDRAGGASVGLIVKDFESDLTSFLDDFSELLNTDRPLAVIGPLLSKNLPVMAEL
                     AERAHIPLITPTATLPNVRRLGNYIFSTALTNQLQAKRIAAYAIKEQGFRRFCILHPD
                     TAYGRELARLFAQEVHQHEGEVIAIESYKEGETDVSAQLKRMKAEDLKKYGLSVPVDQ
                     TNLTKKLTKLDKKILYTPGFDAIFIPGRAADAGIITAQLNFHDMKVPFLGANGWNSPD
                     FARTADSSIDGAVFVDGFFIESPTPTVQDFVDRYKKRFQTNPTLFAMQGYDAAKLVID
                     AVHKGASSGEAVHDQLLTQPDLSSLAGPAAFGPDGTFNRPLFLMQIRRGRFLQVN"
BASE COUNT         1293 a         1646 c         1915 g         1474 t
ORIGIN      
        1 gacagaactg gacgttgttc gatacggaag ttacgtggac cattcagact caggcagagc
       61 tgggtgagga gccaccgctt gcgcgctaat gggattggac ggtagcatgc ggtcatcatg
      121 actacgatga ttcgaaaaac attttccgtc ccgcccctcg gttgtaattg ttcgatcatt
      181 ggagatccgg tgacgaagca ggcgatcgtg gtcgatcctg gtggagcgcc tgagcgaatt
      241 ctccacgagc tgcaccatct cggactcacc gtcagccaca ttctccacac ccacgcccat
      301 ttcgatcatt ttcttgcctc cggcgagatg aagaaggcaa cgggggcgat gatctgtctg
      361 catcaaagcg atcgtgaact gtggaagaat ctcgaattgc aatgccggat gttcggcgtc
      421 gtgtacgtac ccgttccgat gcccgactat tggctgaagg atgaggagag gctgctggtt
      481 ggccaggtct ctgctgttgc actccatacc cccggtcata cccccgggtc catgagtttt
      541 tattttccgg gcgacaacgt ggtcttggcc ggcgacacgc tttttcgcgg cagcattgga
      601 cgaaccgatc tctggggtgg ggacttcgat gccattgagc agtccattcg agagcgcttg
      661 tataccttgg aggatgccac cattgtggta acagggcatg gagcggagac cgaaatcgga
      721 ttggagaagg agacaaatca cataatccgt gcttgaccag ttggggaggt gaccatgatg
      781 gcatgtaagc ggttcgccct ctctctctgt tttctcgttg cacttgtcct gccggttgca
      841 caagccagtg aaccgtcacc agtactctcg gagcaggtca cgatcaccga tgtccaggtt
      901 cgcatctctg atcacggcac tgtggtgctc ttgctggccg aaggcaaggc catcccgatt
      961 tttgtcgatc tgacagttgc gttgtcgatt caaggtgcgt tggacggcga gaagcctcat
     1021 cgccccctct ctcacgattt gatgcatacg atgatggaaa catttggcgc tcacgtgacc
     1081 cagacgatca ttacattgaa ggagggcacg tattacggtg cgctggcagt ggcgtttcaa
     1141 aatgaagtca aggtgttcga cagccgatcg tcagattcca tcgcgctggc catccatttc
     1201 aaggctccga ttctcgtggg acgggattta ctgaactcgg ctgggcgtgt gttgggggaa
     1261 tcgaagacgg aaaaattgtg aggggctatt ctttttccat ccgaacgcgg agccgttcaa
     1321 ggcgtttgtc ggtggactgc ttaatgaagg ccatgttttc tcccaggtgt tgctcggcgt
     1381 tgattttgcc ttcctcgcgt cgcttgagtt ggtccagccc gaattgtgcg atcgaggggc
     1441 cgtcttcctg gttcagcgcc ttccagatcg ccgcacaggt ggcctgtttg gcgacggggt
     1501 acttgtcgca aggtggatcg gctgcccatg cgatgcccca gctcgctccc agcaggatga
     1561 ataccaacgc cggcgcagtc acggtatgtc tactctccgc ataaatggac cctccagctc
     1621 ggcaaacgat agcacaaccc agctggtttt gcagacgatc gatgtgtatg aacggtcggc
     1681 caaggagtgc ctcgcccgct ggagtcagcg acgtcatcgc cgaccgccgt tgttagtcga
     1741 atggttacgg tgtcttcctg ccaatgcacg attgttggat cttggttgtg gtggaggcca
     1801 ggatgcgggc gacctaggtc ggcgtggcta tcgcgtggtg ggcgtggatc ggacgaaggc
     1861 cttgctctct gcgggacgac gtcgatatcg ttcattgccc ttggttcacg ccgatctccg
     1921 tgaccttccc tttcacctga agtcgttcga tggcatctgg gctgccgcct cgctgatcca
     1981 ccttcccaaa gcagatgcac gtcgtgtcgt gaccgatctg tgcaagctta tccgtccagg
     2041 tggcctgttt gccgcaacag tgacccatgg cgtgaagagc catctcatga cggatggatg
     2101 gctccctgga cgctatctcg cccgctggag aaaggaagag tttgctcgcg cagttcgtcg
     2161 agctggatgg aacgtgcttg aactaaaagt cataactaac cgggagcgca aggggcggtg
     2221 gttgaatctc ctcgcgcaga aacaggactg agtcggcctg tgggttactt tgatacaggt
     2281 ggctccgagg ccgtatggcg attgagcgtg agatagacct tgtcgtcgaa ggtatagaag
     2341 ccgccctggt cgatgtcgtg catgatacag aatggtgaga agacgacgag gcagccgaaa
     2401 atatctgcga ccacccacca ggaccaggtg cgatcgattt tgaaagacgt cggttcgaag
     2461 ccttccctgt tcacctgtgc gaggtggtcg cctttgcggc tgagagtgac cgtgcctggg
     2521 gctgtgaggt gcaggtagtt gtcgatgacg accgcggtct ccggcgggtt ggtgaaaatt
     2581 gtcaccgact gtttgtcgga atgcatccag gttccgcagc ctgagagtcc cgcgaccagg
     2641 gcatacagca cgatccctcc ttgccatatc ttccaatttt tcatcttcca ctccatctag
     2701 caggctgcgg aaaaaccgag tttagacatg aaacacccgt ggaatcttac agaacggtac
     2761 tgatgtcaca aagatcgtag gatgctcaaa aatgtctgct atctcaccca cccaacccca
     2821 gcgcgccaag acgcgccttg tcccaagcaa ggccgcaggc gagtcgaaac tggtggactt
     2881 tttcagcatc ctgctagata ttcatcgctt cgttcacgtc atggagagtc gctttggcga
     2941 cctcgccggc ccggcggctg ccttcggtca cgatgtcgtt caggtgcgcg ggatctttgc
     3001 tcagacgtgc gcgcgcgtcc cacatcggcg tcatgcgttc gacgattcta tcggcgacga
     3061 gtttcttgca atcgatacag ccgatggcgg ctgtccggca ttcggtatta atctgctcct
     3121 gaatcatcgg taatgaaaag attttgtgaa aatcatacac cgggcaaatg tccgggttcc
     3181 cggggtcgtg gcgtcggaca cgggcgggat cggtgaccat cgtcttgagc ttctggcgca
     3241 cgaccggttc tggatcggac aggttgatcg tattgttgta gctcttgctc atctttcggc
     3301 catccgtgcc gagcactttc gggaacctgg tgagctgctc catcggttca ggaaacaccg
     3361 gcttgtacag gctattgaac cggcgggcga gttccctggt gagttcaagg tggggcagct
     3421 ggtctttccc taccggcacg aaatcgggct tgtagatgag aatatccgcg gcttggagaa
     3481 ccggataacc gagaaatcca tacgtggtca agtcgcgttc cttgatttct tcctgttttt
     3541 ctttataggt cggattccgc tccagccagg cgataggaat catcatggag agcaggagat
     3601 gcaagacggc atgctccgga atacgcgatt gcacgaacac cgtggctttc gcaggatcga
     3661 tgcccgctgc aagccagtcg atcaacaatt cgcggacgaa ctcccggata cggctggtat
     3721 cggcatagtt cgtcgagagt gcgtgccagt cggcgacaaa aaagtaacac tcgaactcgt
     3781 tctgcagact tttccacttc tccaaggcgc cgagataatt gccgagatgc agcagcccgc
     3841 tcggctgcat gccactgagg actcgtttcc taggtgtggt catggtgtgt ttcccgctcc
     3901 gagactgact gctgtggaga gaattgagtt ggtgaggctg taggtcaagg tgccggcgat
     3961 ggtatggatc acgtgaatct gtgggtcgag tatgattagc cccatcagga tgaacatgcc
     4021 atagggctct acgcgtgaaa gcgccatcgc ggcggtgtaa gggagaagac ttgtgagaat
     4081 cctcccgccg tccaacgggg ggagcggcag cagattgaac agcatcaaga gtacattaat
     4141 caaaacggag taagccgcca tcacggcgat cggctggaga aacatttgct gcaatgtgcc
     4201 tggggaacta gcggcttcgg agttgagcaa atagtccctt atcgtgggat caattgacaa
     4261 caaaattgcg aggaggaggg cgctgccgat ggccagcgcg agattcatgg caggtcccgc
     4321 cgccgcgacg agtgccatgt cgcggcgtgg tcgatgcatc gcgcgtggat cgatcgggac
     4381 cggcttcgcc cagccgagga ggaatcctcc cggcatgagc agacacagca gcggtagaat
     4441 gatggttccg aatggatcga tgtgcgccag cgggttgacg gtgagccgac cctggagttt
     4501 ggccgtcgaa tcgccgcatc gataggcgac ccaaccatgg gcgtattcgt gcaacaccat
     4561 cgcgaagagg agcgggagcg ccatgtagga aatcgtgtgg atgatgtgcg gcagggagtt
     4621 catgcggtgc ctttcttaat tcacctgaag gaatcggcct cgtcgaattt gcatgaggaa
     4681 gagcggacga ttgaaagtcc cgtccggccc aaacgccgct ggccccgcga gggaggagag
     4741 gtccggctgg gtcagaagct ggtcgtgaac ggcttcgccc gacgaggcgc ctttgtgcac
     4801 cgcatcgatc accaatttcg cagcatcata gccttgcatc gcaaagagtg tcggattggt
     4861 ttggaaccgt tttttgtatc ggtcgacgaa atcctgcacg gtcggggtgg ggctctcgat
     4921 aaagaagccg tcgacgaaca cggctccgtc gatcgacgaa tcggccgtgc gggcaaagtc
     4981 cggcgagttc catccattcg cacccaggaa aggcactttc atgtcgtgaa agttaagctg
     5041 ggcggtgatg atgccggcgt cggctgcgcg acctggaatg aagatcgcat cgaacccagg
     5101 cgtatagaga attttcttgt ccagcttcgt cagtttcttg gtcaaattgg tctgatcaac
     5161 cggcacggat aagccatact tcttgagatc ctccgccttc atgcgtttga gctgggcact
     5221 cacgtcagtt tcgccttcct tgtaggactc aatcgcaatt acttctcctt cgtgttgatg
     5281 tacttcttgg gcaaagagcc gggccaattc acgaccatag gcggtgtcgg gatgcaggat
     5341 gcagaatcgg cggaatcctt gctccttgat ggcataggcc gcgatccgtt tcgcttgcag
     5401 ttgattcgtg agggccgtgc tgaatatata gttgccgagt cgtctgacat tgggcaaggt
     5461 cgccgtcggc gtgatcaggg ggatgtgcgc tcgctcggcg agttcagcca tgaccggcag
     5521 attttttgaa aggagcggcc cgatcacggc gagtgggcga tcggtattca gcagttcgga
     5581 gaagtcgtcc aaaaacgaag taaggtcgga ttcgaaatcc ttcacgatga gaccaaccga
     5641 tgcacctcct gcccggtctc tatttgcctc gacagccagt tgaatgccgc tgaggacttc
     5701 attggcaaag ggggcgagtt ttcctgaaag gggcaggacc gaggcgatga aaaactggtt
     5761 ggagtgtagt ttggcttgga gtgtgacaag gagttccgag gttttggccg cctgcgggtg
     5821 tatcggaaaa ttcacgagaa attgttggat ttgccgaatg gcctggtgct cttctccacg
     5881 gccgatgtag aggtcgatca gtcggaggga cgcgagatcg ccaggaaagc tgcgtgggaa
     5941 aaggctccgg atttgctcga gtgctttctt gtcggatgtt tcgctggcca actcgcgaat
     6001 gcgggtccgc acgtcttctg cttggtcttc tgttcccaat gctagttcat cgagccatgc
     6061 ctgaatggca cgggtcgtgt catttttttg cgcgtaaaac tcgccactca actgcagggc
     6121 ttcgcgtttt gtgccgtcat ctttcgaaag gttgcgcagg cctgtcagga tcggcagagc
     6181 aaggtcgaga ttgcccatcg ccgcatggac tcgggcttgc aacagcttgc ctctgtccgt
     6241 cacctcagag ttggggaact cggtttgcag ctgccgcaag tatttcaaag catccgcatc
     6301 ttgtttggcg cggtggagcg cggcgccg
//
LOCUS       DATIPF010000381         5271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_341131, whole genome shotgun sequence.
ACCESSION   DATIPF010000381 DATIPF010000000
VERSION     DATIPF010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341131"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1491
                     /locus_tag="VLL06_09575"
     CDS             <1..1491
                     /locus_tag="VLL06_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31254.1"
                     /translation="TGCAMVAQAQDKKSMPPPHDAVADAQAPRTVLIERPEQRQTAHD
                     RSNVFDAQHARPSSPAFLNQLRDGKASGFDFYRDPLGAENPLQEPDEMMKTDMAKKAK
                     VMETQHTLLDRRYHLEPKLDPDAKMSRGKPLVVGPTARLPKGMTWERLAGESPEEIKQ
                     HGLFPYPSLPHPLQSPGGQVFPQMQIDMFPRLERFDVEFDLPAAFLPEFPPAMFLSNR
                     PELGDVSRGEVVSINNYYRLFKNILTPVQLDGLRMLLTPFPQEEFNPTDDRKSLHPSL
                     GVTCFDCHVNGHTTGQFHLNPDMRPEERRLRLDTTSLRGVFNQQIHSSKRSLRSIEDF
                     TEFEQRTAYFNGDEIHAIKKGMNILDRVQVSHMAQMQNMLDFPPAPKLDPLTGRLDPK
                     KATENELRGETVFFGKGQCEGCHTPPFYLDNKMHDLHLERFLKDEPGDGPIKTFTLRG
                     IKDSPPYLHDGRLFTLEDTVEFFNLVLGVHLSQEEKKDLTAFLRAL"
     gene            1567..2052
                     /locus_tag="VLL06_09580"
     CDS             1567..2052
                     /locus_tag="VLL06_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HSS31255.1"
                     /translation="MWFFKTDDAWSGLIFRIVLGSVIFAHGAQKLLGWFGGEGYAGTM
                     ASFTQQMGLPWVVAFLVVIGESFGSVSLIAGLLTRVTAASYIIIMLGAIATVHWQNGF
                     FMNWFGRQQGEGFEFHLLVMAMSTALVVIGGGKWSVDGMIARLFVRTEISLEQQMSKA
                     A"
     gene            complement(2197..2745)
                     /locus_tag="VLL06_09585"
     CDS             complement(2197..2745)
                     /locus_tag="VLL06_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31256.1"
                     /translation="MRIHIVGIAALSLLSLPSLAFSQDQTVTGDKSVVFPPRFKCSTN
                     SPCRNLTGEILRIEESYWIQDPGGGETHVRVTRDTKMAEIPKVGDKIAAQLTSNGDAN
                     AIVKLAEIPKPKELPVPARAEKDFRENQPGQATASGQAEQGSPTNRRSEAEVSGKVKS
                     LDPLSGGGNIDPSSKMEQPTAR"
     gene            complement(3289..3561)
                     /locus_tag="VLL06_09590"
     CDS             complement(3289..3561)
                     /locus_tag="VLL06_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31257.1"
                     /translation="MKQTAYVTTVLMFAGLVALGIWQAAFQEGLSWAAWLDSWSFSLL
                     SMLLIIGLLISSSALLYSVIRRLWNAEPREESRNNGGTEKKNQKTA"
     gene            3591..3863
                     /locus_tag="VLL06_09595"
     CDS             3591..3863
                     /locus_tag="VLL06_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31258.1"
                     /translation="MSGAVNYAFRVPLDTQKYGRGDLLTDLDDSVRMDMKVPTKRVPS
                     VTRIELAASMRYRMELVLSVHGLRYELATSSKHISLPETRIKTMNP"
     gene            complement(3899..4156)
                     /locus_tag="VLL06_09600"
     CDS             complement(3899..4156)
                     /locus_tag="VLL06_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31259.1"
                     /translation="MAELKLTASSAIDLLDPSNCLSIATAKPGQEIRVCVRSEGKLME
                     GVGTLSNDGKSFSVVVTREDGVQEIHNVSYRDLLKGLSDDD"
     gene            4461..4820
                     /locus_tag="VLL06_09605"
     CDS             4461..4820
                     /locus_tag="VLL06_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31260.1"
                     /translation="MCLHKALILAEQVTYERIHGPVASHSELLQLVLNDSWFTWIHPF
                     SLLVQRIDQLLDDDESELWLVDVKDLWILVRSFTRLSEEGDGFARSYYEALLREPDVV
                     CAHMKVISLLTCMSKRC"
BASE COUNT         1200 a         1420 c         1384 g         1267 t
ORIGIN      
        1 accggttgtg ccatggtggc ccaggcacag gataaaaagt cgatgcctcc gccccacgat
       61 gcggtggccg acgcgcaagc gccgcgtacc gtactgattg aacgtccgga acagcgccaa
      121 accgcgcacg atcgttcgaa cgtgttcgac gctcaacacg cgagaccctc ttcccctgcc
      181 ttcctgaacc agctcaggga cggcaaggcc tcgggcttcg atttttaccg cgatccgttg
      241 ggtgcggaga acccgcttca agaaccggat gagatgatga agaccgatat ggcgaagaag
      301 gcgaaggtga tggagacaca acacacattg ttggaccgcc gttatcactt agaaccaaag
      361 ctggatcccg acgccaagat gtcgcgtggg aaaccgctgg tcgtgggtcc caccgcccgg
      421 ttgccgaagg ggatgacgtg ggagcggctc gcgggagaga gtcctgaaga gatcaagcag
      481 cacggtctct tcccctatcc gtccctcccg catcctctgc aatcccccgg tggccaggtc
      541 ttccctcaga tgcagatcga catgtttccg cggcttgagc ggtttgatgt cgagttcgac
      601 ctcccggccg cgttcctgcc ggaatttcca ccggcgatgt ttttaagtaa ccgcccagaa
      661 ttgggcgatg tctcccgcgg tgaagtggtc tccatcaata attactatcg cctcttcaag
      721 aatattctga ctccggtcca gctcgatgga ctcaggatgc tgctgacgcc ctttccacag
      781 gaggagttca acccgaccga tgatcgcaaa agcctccatc ccagtctggg cgtgacctgt
      841 ttcgactgcc acgttaatgg acatacgacg gggcaattcc atctgaaccc cgatatgcgc
      901 ccagaggagc ggcggttgcg gctcgatacc accagccttc ggggcgtctt caatcaacag
      961 attcacagct ccaaacggag cctccggtcg attgaggact tcacggaatt cgagcaacgg
     1021 acggcgtact tcaacggcga cgagatccat gccatcaaaa aagggatgaa cattctcgat
     1081 cgggtccagg tgagtcatat ggcacagatg cagaacatgc tggatttccc accggcgccc
     1141 aagctcgatc ccctgaccgg tcggcttgac ccgaagaagg ccacggagaa tgaactgcgc
     1201 ggggagacag tctttttcgg caagggccag tgtgagggat gccatacccc accgttttat
     1261 ctcgataaca aaatgcacga tctccatctt gagcgcttcc tcaaggacga acccggcgat
     1321 ggccccatca agacgtttac gctccggggc atcaaggact cgccgcccta cctgcatgat
     1381 gggcggctat tcacgcttga ggatacggtg gagtttttca atctcgtgct gggggtgcac
     1441 ctcagccagg aggaaaagaa ggatctcacc gcatttctga gagccttata ggggcgcgca
     1501 ttctgtccgt agtccagcaa gacccgtcgt cggcggtcga tgtcgccgac tacactaaag
     1561 gacgacatgt ggttctttaa gacagatgat gcttggagtg gcctcatttt ccggattgtg
     1621 ctgggcagcg tcatcttcgc gcatggggcg cagaaactgt tggggtggtt cggaggcgaa
     1681 ggctatgcgg gcacgatggc ctcctttact caacagatgg gacttccgtg ggtggtggcc
     1741 tttctcgtgg tgatcggcga gtccttcggc agtgtgagcc tcatcgcggg actactcacg
     1801 cgcgtcacgg cagccagtta tatcatcatc atgcttgggg cgatcgcgac ggtgcattgg
     1861 cagaatggat tttttatgaa ctggttcgga cgtcagcaag gggagggatt cgagtttcac
     1921 ctgctggtca tggccatgag cactgccctc gtagtgatcg gcggaggaaa atggtcggta
     1981 gacggaatga ttgctcgcct attcgtgaga actgaaatca gtctggaaca acagatgagt
     2041 aaggccgcat aaggcatgaa ggtacacgtc tttgtatcta tcgacacacc cgcacaggaa
     2101 gccaaccttg agttatggtc attatggtca cttcacgtct cgttctattt cttttccgct
     2161 tgaacccgcc agcctcagcc tccggtttct catccctcac cgagctgtcg gctgctccat
     2221 tttagaactg ggatcgatat ttcctccacc cgatagcgga tcgagacttt tcaccttgcc
     2281 actgacttcg gcctcggacc gtctattggt agggctccct tgctcggcct gaccgctggc
     2341 tgttgcttga ccgggttggt tctctcggaa atccttctca gcacgtgccg gaaccggtaa
     2401 ttccttgggt ttcgggattt cagcaagctt tacgattgca ttggcatctc cattggaggt
     2461 cagttgtgcg gcgatcttat cgccgacttt cggtatttcc gccatcttgg tatctctggt
     2521 caccctcacg tgggtttccc ctccaccagg atcttgtatc caatacgatt cttcgatgcg
     2581 caaaatttct ccggttaaat tgcggcaggg cgaattcgtc gagcatttaa atctcggggg
     2641 aaataccact gacttatctc ccgtcaccgt ctgatcctga ctgaatgcca gcgagggcaa
     2701 gcttagtaag ctcaatgcag caatcccaac aatgtggatt ctcattgcat cctcctttta
     2761 gtgtttactc ttcgattatg gtgttatgca ttcgcggcct gatatcaact ttctaactcc
     2821 ctcttccctt taaagattac cgttcgacag gcgaggaatg aactcgtaaa agcccgagcc
     2881 aatgctggct gttcaccgct gcagcgttct gcgatagcat cttcaggcat aggctattcg
     2941 gcatgatgga tggtgagctc tatccatgca agaccgtgac gttcgagcag acttatcggc
     3001 tcctggtgag atgaagggag gggccgatcc gaagttggca aggagaggtg caatggcacg
     3061 gcgtgacacg gataaccgcg aggtcgtgag ggcgttgcag cgaatgacca atgccgttga
     3121 cgacttcgcg ggctgccttt attggatgcc ataatccatg cggaattgat tctctcagtt
     3181 catcgattgc ccaacgatca aaaacaagga tcggaactgg cggctcgagc gaaacgcaat
     3241 tccccgcctg aacgacgtgc tgtttctttt tccgttcacc gaccacattt acgccgtttt
     3301 ctgattcttc ttctcagtcc ctccattatt cctactttcc tcgcgtggtt ctgcgttcca
     3361 gaggcgcctg atgacagaat atagtaaggc actggagctt atcagcaaac caatgatgag
     3421 caacatgctc agaaggctga aggaccagct gtcaagccat gccgcccatg atagaccttc
     3481 ttgaaaggca gcttgccaaa taccaagagc aactagtccc gcaaacatca gaacagttgt
     3541 gacatacgcg gtttgtttca tagttgcctc cttttcttat agataattcc atgtctgggg
     3601 cagtcaacta tgctttccga gtacctctgg acacccagaa gtatgggcga ggcgacctct
     3661 taaccgatct ggatgattca gtgaggatgg acatgaaggt cccgaccaaa cgtgtcccaa
     3721 gcgtgacgcg cattgagctg gcggcctcga tgcgataccg catggaattg gtgctctcgg
     3781 tccatggatt gcgttatgaa ttggccactt catcgaaaca catatctctg cctgaaacac
     3841 gaattaagac catgaatcca tgaattagtg gaaacactgg aacaacaaat ggccacaatt
     3901 aatcgtcatc tgagagcccc ttcaagaggt ccctgtagct tacgttatgg atctcctgca
     3961 caccatcctc tctggttacc acgacagaga aagacttccc gtcattactg agcgtgccca
     4021 cgccttccat gagcttgcct tcggagcgca cgcacactcg aatctcttga ccaggcttcg
     4081 cagtcgcaat gctcaaacag ttcgatggat ccagaaggtc tatggcagag gatgcggtta
     4141 atttgagttc ggccatgtca gtccctccag ctagagcggg ggatcgttgg atgcatttcc
     4201 gctgcggttc aacggtgcct atgtttgttt gtcagcaatc tgtacatgta cctaacaact
     4261 tattgaccta gacaatcccc tagaaatggg gtgaagggtc ttaggcggcc gccgctctat
     4321 ctttctgccg cttgggacgt caagggaatc acccagctat gcgggatcaa atcacatgat
     4381 aagtgaacac tgtaatgcca acgagttcgg ctgagacatc ttgccttcgt cagagcctgc
     4441 tcgccatgcg gagcagtctc atgtgtctcc acaaggctct catcctcgcg gaacaagtga
     4501 cctacgagcg cattcatggg cccgttgcct cacatagcga actcttgcaa ttagtgctga
     4561 atgattcgtg gtttacctgg attcatccct tctctttact ggtgcagcgg atcgatcaac
     4621 ttctggatga tgacgagtct gaactatggc ttgttgatgt gaaggacttg tggatccttg
     4681 ttcgatcctt tacgcggctt tcggaggagg gagacggctt tgcacggagc tactatgagg
     4741 ccctgctacg agagcccgat gttgtctgcg cccacatgaa agtcattagc cttctcactt
     4801 gtatgtccaa gcgatgctaa taatcgatac gaagatcggt atccaccatc actgacattg
     4861 cccagtgacg cacattgatt gccatcaatg cagcaggatc cagacgtgaa attaggccgg
     4921 atgctgcacg gataagacat cggcaaatcg atgatgtttc attttaaaat aaaacagaca
     4981 ccgtgtttct cggcatgctc gaagaagcta ttgaacagat gatcgacgca attaatttgg
     5041 aggagccgac tgaagaggaa gcatttgctg aacatatgcc tcaccgcact ccatttttta
     5101 cggcacagta ggcaaaagag ccgctccagg catcgatgag ggacgagaga gcgattgctg
     5161 acgtatacca aggcagatcg tgtgaaccta ccaccaagac gtggaaggcg tgagcaccaa
     5221 cccgtgaatg atgcatttct tgtggtctcc gtcttcaatg gcaataccat t
//
LOCUS       DATIPF010000382         3370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_341179, whole genome shotgun sequence.
ACCESSION   DATIPF010000382 DATIPF010000000
VERSION     DATIPF010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341179"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..455)
                     /locus_tag="VLL06_09610"
     CDS             complement(141..455)
                     /locus_tag="VLL06_09610"
                     /inference="COORDINATES: protein motif:HMM:NF013671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HSS31261.1"
                     /translation="MIAVTIAAIAKLKAIQTHHPEDPIVRISVRDVDNSRLTFSITLE
                     SNTEPDDVMQQVDGLTIAMDRLSATRMEGVTVDYTESDGFRFQHGGEGRSLPLLTIPT
                     LN"
     gene            complement(452..715)
                     /locus_tag="VLL06_09615"
     CDS             complement(452..715)
                     /locus_tag="VLL06_09615"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HSS31262.1"
                     /translation="MAWYQLAYRPAILKDLANLEPSTAQRLMDKTKWIASNIDNLRHE
                     PIATDLPGLSKYAVEDWRIFYSIDREDQLVDIHGFIRQKELRS"
     gene            complement(723..926)
                     /locus_tag="VLL06_09620"
     CDS             complement(723..926)
                     /locus_tag="VLL06_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31263.1"
                     /translation="MDISQLSQEQFKKLLHGMIDDRLRELLGDPDLGLQLGDSLRARL
                     KESLSSKERLSGEDVAEQLGLRW"
     gene            complement(965..1282)
                     /locus_tag="VLL06_09625"
     CDS             complement(965..1282)
                     /locus_tag="VLL06_09625"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS31264.1"
                     /translation="MTKPRQHLRYKVEFPATFAGDHAGMGIVYNLGVGGCKMVSHRKV
                     KIGDLLSVHLNVPEQNTPISILAAKVQWVLEHGFGVEFLEIQKVEQARLERFLDAQAH
                     LAT"
     gene            complement(1481..1807)
                     /locus_tag="VLL06_09630"
     CDS             complement(1481..1807)
                     /locus_tag="VLL06_09630"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS31265.1"
                     /translation="MVTRKYPRFSVSLSSTLLYKDHFRHTGSVLNVSAKGCRVTSIIS
                     PFTGMQLTVQLHVPGESNPIVINNAAVRWSGSAGIGLEFLTVAPPHQDRLDRMIQQLQ
                     PKTDPR"
     gene            complement(1829..2146)
                     /locus_tag="VLL06_09635"
     CDS             complement(1829..2146)
                     /locus_tag="VLL06_09635"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS31266.1"
                     /translation="MSEQRKYVRYQAEFSATFEGDHAGIGIVYNLGMGGCKIVSNHEV
                     KNGALLTLHLKVPKQVTAITIRAAAVQWILELEFGVEFLEMQRPERDRLEQFLATQVN
                     IAP"
     gene            2420..3172
                     /locus_tag="VLL06_09640"
     CDS             2420..3172
                     /locus_tag="VLL06_09640"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HSS31267.1"
                     /translation="MPTTPDSRTSSQRRKKRSKKSFGLDTLQKRRFQTRLLKWYEKHG
                     RDQPWRKTSNPYHILVSEVMLQQTQVDRVIPKYHEFLGRYPSFEELADAPVSDVKKTW
                     YPLGYNIRPERLHSIACETVERYGGRLPSDAEELLSFKGIGRYTAGAIRSFAFNEDAP
                     ILDTNVIRVLHRVFMAKGDPKTQKARLWQLSEALIPRGKGYDFNQAIMDFGATCCTAR
                     NPACGQCPMKSLCKTYPFNQKSRKAAVRHEGC"
     gene            3159..>3370
                     /locus_tag="VLL06_09645"
     CDS             3159..>3370
                     /locus_tag="VLL06_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HSS31268.1"
                     /translation="MKVVNVAAGLVYHEGRYLIARRNPGVHLAGFWEFPGGKCEPGET
                     LEECLGRELFEELGIRIEVPIPFQVVR"
BASE COUNT          738 a          812 c          995 g          825 t
ORIGIN      
        1 cgccattccc tgggacaaga ttgcattccc cgttgtgaag gacgcgttgc gtcgctatgt
       61 cgacgatgtg gcacggggcg agtttcaggt gcatgtggcc agcttgccgg accgcctacc
      121 atagttttca acaagctgca tcagttaagg gttgggattg tcaggagtgg caaactgcgg
      181 ccttcgcccc cgtgctgaaa gcggaagccg tcgctctccg tgtagtctac ggtcacgcct
      241 tccatccgtg tcgcactcag ccgatccatc gcaatcgtga ggccgtcgac ttgctgcatg
      301 acatcgtcag gctcggtatt cgactccaag gtgatactga aggtcaagcg tgaattatcc
      361 acgtcgcgta cggaaatccg gacgatcgga tcttcaggat gatgagtttg aatcgctttc
      421 agctttgcga tggcggcaat ggtgacggca atcatgaacg aagctctttc tggcgaatga
      481 agccgtgaat atcgaccagc tgatcctccc ggtcgataga ataaaaaatt cgccagtctt
      541 cgaccgcata tttggaaaga ccaggcaggt ccgtcgcgat cggttcatgg cgcaagttat
      601 cgatgttgga ggcgatccat ttggtcttgt ccatgagccg ctgtgccgtg gagggctcaa
      661 ggttcgcaag gtctttgagg attgccgggc ggtaggcaag ttgataccag gccatatcgt
      721 ccttaccagc ggaggccgag ctgctccgcg acgtcctcac cggacagtct ttccttactg
      781 gataacgatt ccttcaggcg ggcgcgcagg ctatcgccaa gttggagtcc gaggtcagga
      841 tcgccgagta gctcgcgcag gcgatcgtcg atcatgccgt gcaggagttt tttaaattgt
      901 tcttgagaga gttgggagat gtccatcaag acggaggata cgaaggcgag tacctaaaat
      961 gcaactatgt tgcgaggtgg gcttgggcat cgagaaatcg ctccaggcgg gcctgttcta
     1021 ctttctgtat ttcaaggaat tctaccccga acccatgttc cagaacccac tgaactttgg
     1081 cagctaggat ggagatggga gtgttttgct ctggaacatt cagatgcact gagaggagat
     1141 cgccaatttt taccttacga tggctgacca tcttgcagcc acctactccg agattgtaga
     1201 cgattcccat cccggcatga tcgcctgcga aggtcgcagg gaattctacc ttgtagcgaa
     1261 ggtgctgcct cggtttcgtc atggatgtcc ctgaagacat agttgctgtt gatgaagcct
     1321 cgcaatgagg ctcccgctga tatcgtcttt gtttcaggaa agattagttg gtgaacggaa
     1381 ggttgtcaaa gaaatacaga gctttggaaa tgtctcgtgt ccacatactt gggagggcta
     1441 ggtggggcga gactgatgaa tatccaagag ggtcggccgt ttagcgaggg tcggtcttgg
     1501 gctggagctg ctggatcatg cggtccaatc gatcttgatg cggaggcgct accgtcaaga
     1561 actcgaggcc gatgccggcg gacccggacc agcggactgc ggcattgttg attacgatgg
     1621 gattggattc gcccgggaca tgcaattgca cggtcagctg catgccggta aaggggctga
     1681 tgatactggt gacacggcat cccttggccg aaacgttgag cacggaccct gtatggcgga
     1741 agtgatcctt gtacagcaag gtactggaga gcgacacaga aaatcgagga tatttcctgg
     1801 tgaccatagc gactctttga gcgattcctt atggggcgat attcacttgg gttgccagaa
     1861 attgttctag gcggtcccgt tccggcctct gcatctcaag aaattctacc ccgaactcga
     1921 gttccagtat ccattggact gcggcggctc ggatggtaat ggcggttacc tgttttggaa
     1981 ccttgaggtg tagggtaagg agagctccat tcttgacctc atggttgctg acgatcttgc
     2041 agccgcccat cccgaggtta tatacgatcc caattccagc atgatcgcct tcgaacgtcg
     2101 cagagaattc ggcctgatag cgaacgtact tcctctgttc tgacatgtgg tctgtcatcg
     2161 aggtgctctc aaaaaggcgt ccggttctca cggtgccttc ctgtgcttcc actcacgttg
     2221 tcctcgcctt tggaaaggct agtcggtgtg tgggccgttg tcaaagaaat agagaacttt
     2281 ggatctttcg gctcgcacgg agttggagag gagagagcta gtgcccgacg gatggcggca
     2341 actgcacgtg ttccgtctag ttgccatttg attttcggtg ggtgcacttc tagaatgccg
     2401 ccattggatt gtcgttgtca tgcccaccac ccctgattct cgaacaagct ctcaacgcag
     2461 gaagaagaga tcgaagaagt ccttcgggct cgacacctta cagaagcgcc gttttcagac
     2521 tcggttgtta aagtggtacg agaaacatgg ccgcgatcag ccgtggcgaa agacctccaa
     2581 cccctatcac attttggtct ctgaagtgat gctgcaacag actcaggtcg atcgggtgat
     2641 cccgaaatac catgagtttt taggtcgata cccaagcttt gaagagttgg ctgatgctcc
     2701 ggtctcggat gtgaagaaga cctggtaccc gctggggtac aatattcggc cggagcggtt
     2761 gcatagtatc gcatgcgaga cggtggagcg gtatggaggc cgacttccga gcgatgcgga
     2821 ggaactactg tcgttcaagg gaatcggacg gtacaccgcc ggcgccattc gatcgtttgc
     2881 gtttaacgag gatgcgccga ttctggatac gaatgtcatt cgggtccttc atcgtgtgtt
     2941 catggcgaag ggtgatccca agactcaaaa ggcgaggctg tggcaattgt cagaagcgtt
     3001 aataccacgt gggaagggct atgactttaa ccaagcgatt atggattttg gagcgacctg
     3061 ctgcacggca agaaatcccg cttgtgggca atgtccgatg aaatcattgt gcaagacata
     3121 ccccttcaat cagaaatcgc ggaaggcggc ggtgcgccat gaaggttgtt aacgttgccg
     3181 ctggtcttgt ctatcatgag ggccgctact tgatcgctcg acggaatcca ggcgtgcatt
     3241 tggcaggttt ctgggagttt ccaggaggga aatgcgagcc tggtgaaaca ttggaagaat
     3301 gtctaggacg ggagttgttc gaagagctgg gtattcgtat cgaggtgcct ataccgtttc
     3361 aagttgttcg
//
LOCUS       DATIPF010000383         3521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_73530, whole genome shotgun sequence.
ACCESSION   DATIPF010000383 DATIPF010000000
VERSION     DATIPF010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73530"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VLL06_09650"
     CDS             complement(<1..855)
                     /locus_tag="VLL06_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31269.1"
                     /translation="MDQSAGLIRQKQTSARCPFDGAQRLWGFMSKDLAKTIHVSIGIV
                     SLVVFGLMGCNLDSSQTDATGSGTAGPNHPPAIRSITLAPLPIVRDGMVTATVDAEDL
                     DGDALTFTFKWIVNDEPRSEEVLPTIHPERLHLGDRLAVEAIPHDGKVAGPAVRSQSV
                     VVGNTPPTIRALTIQPSSAKVGDRLVATVDGSDIDGDEIRYTYRWSHNNRLIGEGEQG
                     TLDTTGFSRGDVVAVSATPHDRGSHGKEQLSELLTLANSPPKFTSSAPGILTHGLFGY
                     VASAVDPEN"
     gene            complement(834..>3521)
                     /locus_tag="VLL06_09655"
     CDS             complement(834..>3521)
                     /locus_tag="VLL06_09655"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HSS31270.1"
                     /translation="ETKLAAAFAAILNQTSSGTAASVLSSSTSGEGAIYQSYFYPTQF
                     EAEREIKYAGYVQSLFVDSYGNFREDTVKDNRLVLTEDRIVVTRYDSVGDKLAIDVYV
                     DANGDGKADPTRDTSVPPDGILDQAFCDDAPNQCDLALANINPIWEGGRSLALMTPSN
                     RKIFTWVDLDNNKLVKNISPPTGAGTDEYISFDSTNLTKLQGYLNLSGAPAAYTAANI
                     VDFIRGTQVTGLSDRTLTGKDTSGAAVSAVWKLGDSVYSTPVVVGAPRERYDILYGDS
                     TYTLFYSMYRNRRQVAYLGANDGMMHAFNVGFFHEGDDTVGTPAKVEHGWFTNATTAD
                     GRGVNIGDELWGFIPQELLPHLRWLADPSYTHVYYVDLKPKVTDVRIFDPADPDHPGG
                     WGTVLIGGFRMGGSCGNCASGQPGQKMNFAANFDNIAGVDAARDFLSAYFVLDVTNPE
                     KVPTLLWSFSDTNLGFTMSYPAVARVSPLTDLHKYDSGNEKWFMIVGSGATSYDAGVG
                     QTGKMFAVDMKARMTSTAAGVVTTFDASGGGATPPNSFIGDLSSFDRDFDYRADVIFG
                     GKVISAAPWEGKLIRLTTGCWKPAAPTCNTNPSAWGVPSGTAGVQAPSEILYQFRDGG
                     GTTRTLGPIPTAVGVTIDSTGNTWVFAGSGRYYTQISGTGDKIDTSAQYLIGIKDSVM
                     QGSGGCAGGDVSITGCILSSTINNELIDMSTATICQLGTGTCDGTTTQQVTTVPAMPT
                     GGTYASLISLVQSKKGWFTKLTVPAGGLPSERAVANPVLVGGIVFFPTFTPSADVCVA
                     AGSSNLYALYYVTGGAYSSPVVGMTGQNINNKTSLGEGLATTVAIHLGAQGTGTTGAG
                     STSGMIGCSQSSTGAINCVNAGTASGTSSRYLSWINQRD"
BASE COUNT          760 a         1023 c          952 g          786 t
ORIGIN      
        1 attttcgggg tccaccgccg atgcgacgta gccaaatagc ccgtgggtca agattccagg
       61 agcggatgag gtgaattttg gaggactatt agccagagtc agcagctccg atagctgttc
      121 ctttccatga ctgccacgat cgtgaggtgt tgcagaaaca gccacaacgt cgcctcgtga
      181 gaaaccggtt gtgtccaacg ttccctgttc gccctctccg atcaatcgat tattgtgtga
      241 ccatcggtac gtgtagcgga tctcatcccc atctatatcg ctgccgtcca ctgttgcaac
      301 gagtcggtct ccaaccttgg cgctgctggg ctgaatggtc agagcacgga tagtcggcgg
      361 cgtattaccg accacaaccg attggctacg gacggccgga cccgcaacct tcccatcatg
      421 aggaatggcc tcgactgcca gtcgatcacc gaggtgcaga cgttcgggat gaatcgtggg
      481 caagacctct tcggacctcg gctcgtcgtt gacgatccac ttgaatgtga atgtcagggc
      541 atctccatcc aagtcttcag cgtcgaccgt tgctgttacc attccatccc gaactatcgg
      601 caggggagcg agggttatcg agcgaatggc tggtggatgg tttggcccgg cggtaccgga
      661 acctgttgcg tccgtctggg aggaatcgag gttgcacccc atcagtccaa aaacgacgag
      721 agatacgatt ccgatggaga cgtgtatggt cttcgccagg tccttcgaca tgaagcccca
      781 caacctctgc gcgccatcga acgggcagcg cgcagaggtc tgtttctgtc ggatcaatcc
      841 cgctgattga tccatgagag atatcgactc gaggtgccag aagccgtgcc ggcattcaca
      901 cagttaatgg ctccggtact ggactgagag caaccgatca tgccgctggt gctaccagct
      961 ccggtcgtgc cggtaccttg ggctcccaag tgaatggcta ccgtcgttgc caacccttcg
     1021 ccaaggctgg tcttattgtt gatgttctgc cccgtcatcc cgaccacggg cgacgagtag
     1081 gcgccgccgg tgacgtagta gagggcatac agattactcg accctgcggc cacgcagacg
     1141 tcggcactcg gcgtgaaggt cgggaagaag acgattccgc cgaccagaac tgggttggcc
     1201 acggcacgtt cgctcgggag gccacccgct ggaaccgtca atttggtaaa ccaccctttt
     1261 ttgctttgca cgagtgagat gagtgacgcg taggtgccgc cagtaggcat cgcaggcacg
     1321 gttgtgactt gctgggtggt ggtgccatca caagtcccgg ttccaagttg gcagatagtc
     1381 gccgtcgaca tatcgatcaa ctcattgttg attgtggaac tgagaataca gccggttatg
     1441 ctgacatcgc cgccagcaca cccccctgag ccttgcataa cggagtcttt gataccaatc
     1501 aggtactggg ccgacgtatc gattttatcc cccgttccgc tgatttgggt atagtatcgg
     1561 ccgcttcctg caaagaccca cgtattgcct gtcgagtcga tcgtgacacc aacagccgtc
     1621 ggaatcggac ccaaggtacg agttgtccct cctccgtctc tgaattgata cagaatttcg
     1681 gagggcgctt gaacaccggc cgtgcctgaa ggaacacccc aggcgctggg attcgtatta
     1741 caggtcggcg cagcaggttt ccagcatccc gtcgtgagtc ggatgagttt cccttcccac
     1801 ggtgccgcgc tgatgacttt ccctccaaat atgacatcgg cccggtaatc gaaatctcgg
     1861 tcgaacgaac tcagatcgcc gatgaaggag ttcggaggag tggctcctcc tcccgaggca
     1921 tcgaatgtgg tcaccacacc ggcggccgtc gatgtcatgc gggccttcat atcgacggca
     1981 aacattttgc ccgtctgtcc aacccctgca tcgtaacttg tggcaccgga ccccacgatc
     2041 atgaaccatt tttcgttgcc gctatcgtac ttgtgtaggt cagtcagcgg gctcacacgg
     2101 gccacggcgg gatagctcat ggtaaatccg agattcgtgt cagagaagct ccagagcaat
     2161 gtcggcacct tttccgggtt ggtgacatcc aacacaaaat aggcgctcaa gaaatcgcgt
     2221 gcggcatcta cccctgcaat attgtcgaaa ttggcagcaa aattcatctt ctggcctggt
     2281 tggccactgg cgcagttccc gcagctgcct cccatgcgga acccgccgat caagaccgta
     2341 ccccagccgc ccggatggtc tggatcagct ggatcaaaaa tacgcacatc cgtgactttc
     2401 ggcttcaagt cgacataata cacatgtgtg taggacggat ctgccagcca tcgtaaatga
     2461 gggagcaatt cctgcgggat aaagccccac agttcatctc caatgttcac cccgcgccca
     2521 tccgcagtgg tggcattggt gaaccatcca tgctcgactt tcgcgggagt gccaaccgtg
     2581 tcgtctccct cgtgaaaaaa gcccacgttg aaggcatgca tcatcccatc gttggctccg
     2641 agataggcta cctgccgtct attcctgtac atgctataaa acaatgtata ggtggaatcc
     2701 ccgtacagga tatcataccg ttctctgggg gcaccgacca cgaccggcgt cgaatagacc
     2761 gaatctccaa gcttccacac ggcagaaacc gcagcgccag aggtgtcctt gccggtcaat
     2821 gtacgatcac tgagcccagt cacctgcgtg ccgcggatga aatccacgat gttcgccgca
     2881 gtatacgctg ccggggctcc actcagattg agatagccct ggagcttggt cagattggtg
     2941 ctgtcgaatg aaatatattc gtccgtcccg gcaccggtcg gtggggaaat attcttcacc
     3001 agtttattgt tgtcgagatc cacccacgtg aagatcttcc ggttactcgg agtcatcagc
     3061 gcgagacttc gccccccttc ccaaatcggg ttgatattag ccagtgccaa gtcgcactga
     3121 ttgggagcat cgtcgcaaaa ggcttggtcg agaatcccat caggaggaac cgaggtatct
     3181 ctcgtcggat cggctttccc gtccccgttg gcatcgacgt agacatcgat ggcgagttta
     3241 tcacccaccg aatcatatcg tgtgaccacg atgcgatcct cggtcagcac aagtcgattg
     3301 tccttaaccg tatcctctct gaagtttcca tacgaatcaa caaagagact ctgcacatag
     3361 cccgcatact tgatctccct ctctgcttcg aactgggtcg ggtagaaata cgattgatag
     3421 atcgcgcctt cacctgaggt agacgacgac aagacggagg ccgccgttcc cgacgatgtc
     3481 tggttcagaa tcgccgcgaa ggcagccgcg agcttcgttt c
//
LOCUS       DATIPF010000384         3036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_692803, whole genome shotgun sequence.
ACCESSION   DATIPF010000384 DATIPF010000000
VERSION     DATIPF010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692803"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VLL06_09660"
     CDS             complement(<1..258)
                     /locus_tag="VLL06_09660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSS31271.1"
                     /translation="MPPTLKPAITFSEKKDLDPAKLVRLYQQAPWAKGRTLEDAREML
                     RHTDVAVTAWDGDVLIGFGRVLTDFVYRATIWDVIVDKAYQG"
     gene            complement(596..1018)
                     /locus_tag="VLL06_09665"
     CDS             complement(596..1018)
                     /locus_tag="VLL06_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31272.1"
                     /translation="MQTGDVLDAMGGLVAQLKTYAAKLPPIVHLSAVPTGVKPMLEAV
                     EEYEEIVSRFRNQSAGTPYKGLNELLVESLEAFEVGRLLGSVHPLLSVLDHLERMGRD
                     KEIEIGRINETRTAEYRSTLNRILPGNKPELDGAGRGM"
     gene            complement(1067..2032)
                     /locus_tag="VLL06_09670"
     CDS             complement(1067..2032)
                     /locus_tag="VLL06_09670"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HSS31273.1"
                     /translation="MVTEFVVTTGELPKRLDVFLVNREPKLSRSALQRLIGQGRVRIN
                     GQVVKPSQKIRPGDTIAFDIPKAEPLEIKGESIPLEVLYEDDVLLLLNKPAGLVVHPA
                     PGHWAGTLVNALLHHFQTSGGTVSTIGGKERPGLVHRLDKDTSGVMVIAKTDECHRAL
                     SAQFKQHTITRVYEALISGVPKKGHGLIELAIGRDKKERKKFSAQTARPKKSVTEYKV
                     DHRFGKLAAHVLLYPRTGRTHQLRVHLASLGHPILGDQTYGGKKVCTVEGVEIPRVML
                     HARTLGFRHPVTGELQEYTKPFPSDMEQAVQTLTGLTPPHAIRTS"
     gene            complement(2223..2642)
                     /locus_tag="VLL06_09675"
     CDS             complement(2223..2642)
                     /locus_tag="VLL06_09675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HSS31274.1"
                     /translation="MAGRLLLEVVTPERLLLSQQVDEVIAPGSEGEFGVLPGHCHFLS
                     MLRIGELRYRVGDVTAHMAVLWGFAEVTPTKVTVMAEIAEKAEDIDVDRAQASVEKAE
                     QRLKAGGLPSEVKEAEISLEKARLRKKIAERACKTDR"
     gene            complement(2773..>3036)
                     /locus_tag="VLL06_09680"
     CDS             complement(2773..>3036)
                     /locus_tag="VLL06_09680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246977.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HSS31275.1"
                     /translation="LGMDELSEDDKMAVARARKIQRFLSQPFHVAEAFTGSPGKYVKL
                     KDTVRSFKEILDGKYDHLPEQAFYMVGPIEEVIAKAEKMGVKV"
BASE COUNT          637 a          881 c          808 g          710 t
ORIGIN      
        1 gccctggtaa gccttatcga caatcacatc ccagatggtc gcccggtaga caaagtccgt
       61 gagtacccgt ccaaatccaa tcagtacgtc gccatcccag gctgtcactg ccacatcggt
      121 gtggcgcaac atctcacgtg cgtcctcaag ggtccgtcct ttagcccaag gcgcttgctg
      181 gtagaggcgg acgagcttgg ctgggtcgag gtccttcttt tcggagaagg taatggcagg
      241 cttgagggtt ggaggcatct tgtactagat tatctccctg ttataatcac caaaaccacg
      301 cgaagtgtac gaggaacacc atgacaacgc aagtccgtac ctgctccaaa tgctttcaac
      361 tgatgtggct gacagaggac caatatgaac gcctcgatga ggtcaccatt cgaggcaagt
      421 gcccacactg cggggcaaaa gtccgtttct cgcttgtttc gcagggagaa aacgctgctg
      481 gtcctaagat gggacactaa agtaagagcc gtcagcgtcc agcgatcacg tctcagtgct
      541 cagatgtctc ttccaaccat catgccgctt caggagctga ctctaccccg ctcaatcaca
      601 ttccccgtcc tgcgccgtct agctcaggtt tgttgcctgg taaaatccta ttcagcgtag
      661 atcgatactc ggcagtacgc gtctcattga tccgtccgat ttcgatttct ttgtctcgac
      721 ccattcgttc aagatgatca agcaccgaga gcagcggatg gacggaaccg agcaatcgac
      781 cgacttcgaa ggcttcgagc gattccacca ataattcgtt caggcccttg tagggagtcc
      841 cggcgctctg gttccggaat cgggagacga tctcctcata ttcctcaact gcctcaagca
      901 tgggcttcac accggtgggt accgctgaca aatggacaat gggcggcaat ttggccgcat
      961 atgtttttag ctgagccacc aatccgccca tcgcgtctag cacatcgccc gtctgcatga
     1021 attcatcctt cgaggcggca ttgcttacgc cacgttgagg ggcctgtcat gatgttcgga
     1081 ttgcgtgagg aggtgtgaga ccagtgaggg tctgaacggc ctgttccata tcggacggaa
     1141 acggctttgt atactcctgc aactcaccgg taacggggtg gcgaaaccct agcgtcctcg
     1201 catgcagcat cacgcggggg atctcgacac cttccacagt gcagaccttc ttccctccat
     1261 aggtctgatc tcccaaaatc ggatggccca gcgatgccag atggacgcgg agttggtgag
     1321 tccggcctgt gcgtgggtac aagagtacgt gggccgccaa tttgccaaag cgatggtcga
     1381 ccttatattc cgtcacggat ttctttggtc gcgccgtctg ggcagagaac tttttccgtt
     1441 cctttttatc acggccaatt gcgagttcga ttaacccatg tcctttcttg gggacgcctg
     1501 aaatgagagc ctcatagacc cgtgtaatag tgtgctgctt gaattgagcg gaaagagcac
     1561 gatggcattc gtctgtcttg gcgatcacca tcacgccgga ggtatccttg tccagccgat
     1621 gaaccagccc tggtcgttct ttcccgccaa tcgtggagac ggtgccgccg gaagtttgaa
     1681 aatgatggag gagggcattc accagcgtgc cggcccaatg gcctggcgca ggatgcacca
     1741 ccaagccggc cggcttattt aagagcagca acacgtcgtc ctcgtaaaga acctcgagtg
     1801 ggatcgactc tcccttgatt tccagcggct ctgcttttgg aatgtcaaag gcgatggtgt
     1861 cgccggggcg gatcttttga ctaggcttga cgacctgacc attgatccgc acacgtcctt
     1921 ggccaatcaa ccgttgcaag gctgaccgcg acaattttgg ctcacggttg accagaaaga
     1981 catccagccg tttcggcagc tcgccggtag ttacaacaaa ctcggtcacc atggacgaat
     2041 caccctcacg ttcaaaccac gctactgcag cgcaaggttc aattgcaatc gggagcgcct
     2101 gatcaaccat aggcctgtcg gtcgaattgt cgcttctttt gaattgaacc acgcggagca
     2161 ccgcaagtct gacaggtcgt cagtcgccga tcggcctgtt gttgaataat ctgagaaaga
     2221 tgtcagcggt cggtcttgca ggcgcgttcg gcgatcttct tgcggaggcg ggctttttcg
     2281 aggctaattt cggcttcttt gacttcggac ggcaggccgc cagccttcag acgctgttcg
     2341 gccttctcca ctgacgcttg cgcgcggtcg acatcgatgt cttcggcctt ctcggcgatt
     2401 tcagccatga ctgtgacttt tgttggagtg acctctgcaa agccccaaag gaccgccata
     2461 tgggcggtca catccccgac gcgatagcgc agttcaccaa tgcgcaacat ggaaaggaag
     2521 tgacagtgtc caggcaacac accgaattca ccctctgagc ctggggcaat cacctcatcc
     2581 acctgctggc tcaagagcaa cctctccggc gtcacaacct cgagcaataa ccttcctgcc
     2641 atgtctctcc tatttcctta tctccactca ggaagtggga ggatcgatgg gtcctctact
     2701 gcgcgcgtcc aacgagggtc ttccgagacc gcgcgttgcg cgagcaagga ggacgcacga
     2761 gtcgcccgct tcctacacct ttactcccat cttttccgcc ttggcaatca cttcttcgat
     2821 cgggccaacc atatagaagg cctgttccgg caggtggtcg tatttgccat cgagaatctc
     2881 cttgaagctg cgcaccgtgt ctttgagttt cacatacttc cctggcgatc cggtgaacgc
     2941 ctcggcgacg tggaatggct gcgagaggaa gcgttggatc ttccgtgcgc gggccacggc
     3001 catcttgtcg tcttccgaca attcgtccat gccgag
//
LOCUS       DATIPF010000385         2526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_781346, whole genome shotgun sequence.
ACCESSION   DATIPF010000385 DATIPF010000000
VERSION     DATIPF010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781346"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..1208)
                     /gene="mdh"
                     /locus_tag="VLL06_09685"
     CDS             complement(270..1208)
                     /gene="mdh"
                     /locus_tag="VLL06_09685"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HSS31276.1"
                     /translation="MGRPKITVVGAGNVGGTTAQRLAEKDEYEVVLVDIVKGVSQGKA
                     LDITQAGPICGYSTRVVGTNGYDETAGSTVAVITSGIPRKPGMSRDELLTTNAKIVQS
                     VVSELVSRSPNVILILVTNPLDAMVHVARRVSGLPKARVMGMAGVLDSARMRAFIAAE
                     LSVPGPDVAAMVLGGHGDTMVPLSRYTTVKGRPVSELMSKDRLEAIVKRTREGGAEIV
                     SLLNTGSAFYAPSASAVSMVEAIVQDQKRVLPCAVLCEGEYGLKDVIVGVPVRLGRGG
                     AEQVVEYELTAEEQASLAASATAVRELCLTVDRLMA"
     gene            complement(1280..1687)
                     /locus_tag="VLL06_09690"
     CDS             complement(1280..1687)
                     /locus_tag="VLL06_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31277.1"
                     /translation="MSHSTERPTVPPWLYKLFTGHQYPYVRRLAKFAQPMKPGEDRQE
                     PTKDLIEAKFWEVYPRCWAKILQEVKVGMIVVFHDLGEYPAGGFQELVDDPDAFLAKT
                     YGKKKIKVNFYDGDNFVCTINFKVAGWTEHEHS"
     gene            complement(1720..>2526)
                     /locus_tag="VLL06_09695"
     CDS             complement(1720..>2526)
                     /locus_tag="VLL06_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSS31278.1"
                     /translation="EEGSGDIVGATLSAHGSEVTALRRFFESLRPPGLRRVPGQADGD
                     ELDVDAAVRMCAERVAGADVSDRIYVRRERKERDVAAAFLVDVSGSTSRQLESGRRVI
                     DLEKEGLVLLCEALEAVGDRYALYGYSGQGRGQVDFLVIKDFDDRLGGKAAQRLSGLA
                     PMQQNRDGAAIRHATAKLLARETRTRLLVLISDGRPLDDGYKDEYSLEDTKAALREAR
                     QRGVHPFCITIDREADGYVRRMYGDVQFAVIDHLEDLPKRLPRIYQRLTT"
BASE COUNT          532 a          875 c          585 g          534 t
ORIGIN      
        1 aatataacaa cccaatccag gtgattcctg ccaagaaatg gacccagcga aagatcaagc
       61 ccacccagtc tgcatcaccg accggcacgc cgaccatagc aaggtacccc atgatcaata
      121 ccacggcgag cccaaatcct acggccatcg tctgcatcgg actctcaaga aatttcatag
      181 ttctcctcgt tcagtgtatg cctacaccgt tgtctgtact gtaggctcgg ctctcctgtc
      241 aagcgcgcct ggaccatctt cgcatgacgt tacgccatta atcgatcaac cgtaaggcag
      301 agttcccgca ccgcggtcgc agaggccgcc aatgaagcct gttcctccgc cgtcagctca
      361 tactcgacca cctgttccgc cccaccacgc cccagcctga ccggcacacc aacgatgaca
      421 tcctttaatc catattcgcc ctcgcagagc accgcgcagg gcaacacccg cttctggtcc
      481 tgtacaatcg cctcgaccat gctgacggcc gaggccgaag gagcatagaa cgcactgcct
      541 gtattcaaca gacttacgat ttccgcaccc ccctctcgtg tcctcttcac aatcgcctcc
      601 agtcggtcct tcgacatcag ctccgagacc ggtcttccct tgaccgtcgt gtacctcgac
      661 agcggtacca tggtgtcgcc atgccctccc agcaccattg ccgccacgtc gggacccggc
      721 acactcagtt cagcggcgat gaatgcacgc atgcgggcgg aatcgagtac ccccgccata
      781 cccatgactc gcgccttcgg cagtccactc acgcgacgcg ccacatggac catggcatcc
      841 aatgggttcg tgacgagaat caaaatgacg ttcggcgatc gggagaccag ctcggacacg
      901 actgattgca cgatcttcgc attcgtggtc aacaactcgt cgcggctcat ccctggcttg
      961 cgagggatcc ccgaggtgat gacggcaacc gttgagccgg ctgtttcatc atagccgttg
     1021 gtgcccacca cgcgggtact atagccgcag attggcccag cttgcgtgat gtcgagggcc
     1081 ttaccttggg agacaccctt gacaatatcg acaaggacca cctcgtactc gtccttctcg
     1141 gccagccgtt gggccgtcgt gcctccgacg ttcccggcgc ccaccaccgt gattttcggt
     1201 ctccccatca cctcacctcg tgcagcgtaa aacgtgaaac gtgacacgta gaattccctt
     1261 acgtctcatc tttcacctct cacgaatgtt cgtgctcagt ccatcccgcg actttaaaat
     1321 tgatcgtgca gacgaaatta tccccgtcat agaaattgac ctttatcttc ttcttgccat
     1381 aggtcttggc cagaaaggca tccggatcat cgaccaattc ctgaaatcct cccgccggat
     1441 attcgccgag atcgtggaag accacgatca ttcccacttt gacttcttga agaatcttgg
     1501 cccaacaacg gggatagact tcccaaaact tggcctcgat caggtccttc gtcggctctt
     1561 gtctatcctc ccccggcttc ataggctgtg cgaacttcgc cagccggcgg acatagggat
     1621 actgatggcc cgtgaacagc ttgtatagcc agggcggcac ggtcggccgt tcagttgaat
     1681 gtgacatcaa tgctcgatac ctctctcccc taaaatcttc tacgttgtca gccgctgata
     1741 gatccgcggc aatcgcttcg gcaaatcttc gaggtgatca atgactgcaa actgcacatc
     1801 cccatacatt ctccgaacat agccatcggc ctcccgatcg atcgtgatac aaaacgggtg
     1861 cactccccgc tgacgcgcct ctctcaacgc agccttggtg tcttccaacg aatactcgtc
     1921 tttatagccg tcatcgagcg gacgcccatc gctgatgagg acgagcaatc gcgtccgcgt
     1981 ctctcgcgcc agcagtttgg cggtggcatg gcgaatggcc gccccatcac ggttttgctg
     2041 catgggagcc agtccgctta aacgttgcgc cgccttcccg cccaaccgat cgtcaaaatc
     2101 cttgatcaca agaaaatcca cttgccctcg gccttggccc gaatagccat agagcgcata
     2161 tcggtctccg accgcctcga gcgcttcaca caggaggact agcccctctt tctccagatc
     2221 gatcacgcgg cggccggact ccagctggcg actggtcgac ccgctcacgt cgaccaaaaa
     2281 cgcggccgcg acatcccgtt cctttctctc ccgcctgacg tagattcgat ccgatacatc
     2341 tgcccctgct acccgttcgg cacacatgcg gaccgcagca tccacatcaa gctcgtcgcc
     2401 gtctgcttgt ccaggcacac gccgcaaccc aggcggccgc agactctcaa aaaaacgtcg
     2461 aagggctgtc acctcgctcc catgcgcgct caacgtggcc cctacgatat ccccgctccc
     2521 ctcctc
//
LOCUS       DATIPF010000386         3352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_781630, whole genome shotgun sequence.
ACCESSION   DATIPF010000386 DATIPF010000000
VERSION     DATIPF010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781630"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VLL06_09700"
     CDS             <1..252
                     /locus_tag="VLL06_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSS31279.1"
                     /translation="AGWLRANGNKTVLIEGHCDERGTAAYNLVLGEKRARAAKTYLQD
                     LGIQSSQLQITSYGETRPFCKQATEDCYQQNRRAHFVTK"
     gene            321..941
                     /locus_tag="VLL06_09705"
     CDS             321..941
                     /locus_tag="VLL06_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31280.1"
                     /translation="MKHYHSVAIGLLGFIIATGCAGKGERIDLKIPIAKGTDEKIVAL
                     SSATVAILPFEDHRSDRSRLGTRHHLWGGESYFSLSSGSLGETTAQAFSDYLKSRGWN
                     VILTKGNGATGADVTISGQLIDGVIDAQSSIGQTTLNARSRLAVQVKNHADGSQVHET
                     LTSTGVNQVFWFDPEDAQELLNELYNKNFEKFLVDTRLDGKLLKLR"
     gene            976..1368
                     /locus_tag="VLL06_09710"
     CDS             976..1368
                     /locus_tag="VLL06_09710"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS31281.1"
                     /translation="MNDHAFSIIPEPYGNKNGASYMDRRAHRRVPAQLQGFLLGNSHE
                     IEAVTLDLSIGGARFDCDLEVYPGKVIRVRLVIPGAEELVSIEDARVQWVGEDMFGVS
                     FRNVQPAELDELEQLIDEYEETEDSGHA"
     gene            complement(2116..2340)
                     /locus_tag="VLL06_09715"
     CDS             complement(2116..2340)
                     /locus_tag="VLL06_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31282.1"
                     /translation="MKRLAAFSTILTLGAPAVALAEEHSGGYRGIGMLYYIFMAAILI
                     YGVNDAFGRKAMYVAAPVIIVAFYFLLPDN"
     gene            complement(2503..>3352)
                     /gene="argJ"
                     /locus_tag="VLL06_09720"
     CDS             complement(2503..>3352)
                     /gene="argJ"
                     /locus_tag="VLL06_09720"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HSS31283.1"
                     /translation="LPVNRIIKGIPQLLSQLSDHGGLDAAQAIMTTDLHPKSVALQDT
                     IDGRLITIGGMAKGSGMIHPNMATMLAYFTTDASITKSALQQALRFAVNESFNCISVD
                     GDTSTNDTVLSLANGFAKNRTIKNGTVAFRRFLLLLTEACQALALAICRDGEGVTKIV
                     NIEVMGATTVAQARQVAQTIATSNLVKTALFGEDANWGRVMAAIGRAGVPITPSKLTV
                     WFGGVKMVQRGVGLGLVAEQHIAKVFRRKEFTITVGLGQGPHRAHMWTTDLSFDYIRI
                     NASYRS"
BASE COUNT          797 a          885 c          901 g          769 t
ORIGIN      
        1 gccggatggc ttcgcgctaa tgggaacaag accgtactga tcgaaggcca ttgtgatgag
       61 cgcggcaccg cggcatacaa cctcgttctg ggtgagaaga gggcgcgagc tgctaaaacg
      121 tatctacaag atctgggcat ccaatcctcg caactccaaa ttacgagcta cggagaaacg
      181 aggcccttct gtaagcaggc gaccgaagac tgttatcaac agaatcggcg ggcacatttc
      241 gtgacgaaat aagacgtcgt gcgtgtttca accgaatcct gccacttatg taagacgatg
      301 gcgacacgaa ggaggcatct gtgaagcact atcatagtgt agcaatcggg ctccttgggt
      361 tcatcatagc gactggctgt gcaggcaagg gagagcgcat tgatctgaag atccccatcg
      421 cgaaggggac cgatgagaag atcgtcgcgt tgagttcagc caccgtcgca attctgcctt
      481 ttgaagacca tcgcagcgac cgatcacgtc tcggcaccag acatcatcta tggggcgggg
      541 agagttattt cagcctttcc agcggctctc tcggcgagac caccgcacag gctttctcgg
      601 actatctcaa aagtagaggc tggaacgtga tccttaccaa aggcaatggg gcaactgggg
      661 cagatgtaac cattagcggc caactcattg atggggttat cgatgcccag agtagtatcg
      721 gacagacgac ccttaacgcg agaagtcggt tggcggttca agtgaagaat cacgctgatg
      781 gtagtcaagt ccatgagact ctcaccagta ctggagtcaa ccaggtcttc tggttcgatc
      841 cagaggatgc tcaggaattg ctcaacgaac tgtacaataa gaatttcgaa aagttcctgg
      901 tggataccag actcgacggc aagctcctca agctccggta aggacgtgtt ctacccaacg
      961 gtaggtaccc acaatgtgaa cgatcacgcg ttcagcatca ttcctgaacc gtatgggaac
     1021 aagaacggtg cgagttacat ggatcgacga gcacatcgcc gggtgcctgc acagctacaa
     1081 gggtttctgt taggcaactc gcatgagatt gaagcggtca ccctcgacct ttcaatcggg
     1141 ggtgccaggt tcgattgtga tcttgaagtg tatcccggca aggtgatccg tgtgcgattg
     1201 gtcattcccg gcgcggagga actagtctca atcgaagatg cgcgcgtgca gtgggtcgga
     1261 gaggacatgt ttggagtctc gtttcggaat gtgcaaccag ccgaattgga tgaacttgag
     1321 cagctcatcg atgaatacga agagaccgaa gacagcggcc atgcatagga gtcagtccga
     1381 gccattcctt tctgctgcgg cagacagcct gatgacatct gcgctgtgct cggcctgaaa
     1441 aatcccacca tattttggga atacgcctcc ggtcttttcg agtctatggc ccggcatctc
     1501 gccatctcct tcgcctcgcc ccgaaggatg acttgcacag actcctagct gcgtgagaga
     1561 tcctggccaa cttttaaaca tctcgttaat gtgtcgatcc ttgactgatc cacattgaaa
     1621 gaatatcgta tccgtgtggg aggagcttaa cgccccgagg gaatatggtt catgaggggg
     1681 tcgagcgttg ggatggtgtg caagcccagc ttgactggga agcctgaagt cccaccaaga
     1741 tctccgccgt acctatgctg tcctcggtag gtgcgcgctt ctcccaccct aatctctcgc
     1801 tcgttctagc tgacacgcgc tccatttcac caccgcagca tgttctgtgt cacttcttta
     1861 gggctcaccg cttgtgctgg tgttcccaga ttggaaaaac gtgaaataat agcgcagctg
     1921 taacacgttg catcctcgcg aacgtgcgac atcaggaatt gaaggagctg tttcgcgacg
     1981 aaggatttcg tatgcgaaag ggctccccaa ttctcaacgg ttccagaaac cggcttggta
     2041 gatggaaatc aaacagacgt gcgctgggga gaataatccg gcccgctctc ttctgtagca
     2101 ccctggtgtc agaagttagt tgtcgggcag taggaaatag aacgcaacga taataaccgg
     2161 tgccgccaca tacatcgcct tcctgccaaa ggcatcgttg acgccataga tcaagatcgc
     2221 tgccatgaag atatagtaaa gcattccgat tcctcgatat cccccactat gttcctccgc
     2281 cagggcaact gcaggggccc ctagggtcaa tatggtggaa aaggctgcca gtcgcttcat
     2341 gtcagtctcc ttctctatgg atgttaagcc ttatgatgcc atgccgatgg tgggtgccat
     2401 tacacagcga aaaacaccct tatgacacta cgatagattc gggccaatat gtcgcatatt
     2461 ttgtctcaag aagtcaatct ggtaccgtga tggatgatta cattacgatc gatagcttgc
     2521 gttaatacgg atgtagtcga acgacagatc ggtcgtccac atatgggctc ggtgaggtcc
     2581 ttgcccaagc cccacggtga tcgtaaactc cttccggcga aagactttcg cgatgtgttg
     2641 ttcagcaacg agtccaagcc ccactcctcg ttgtaccatc ttaaccccgc caaaccacac
     2701 ggtcagcttc gaaggggtaa tcgggacccc cgcccgtcca attgctgcca tcacacgacc
     2761 ccaattggca tcctcgccga acagggcagt ctttaccaag ttcgaggtcg cgatcgtttg
     2821 ggccacctgc cgtgcctggg ccacggtggt tgctcccatc acttcaatat tgacgatctt
     2881 cgtcacgcct tccccgtcac gacaaatggc caacgcaagt gcctgacagg cttcggtcag
     2941 gagcaggagg aagcgacgga aagcgactgt accatttttg atcgtgcggt tcttcgcgaa
     3001 gccgttagct aaactcagga cggtatcgtt ggtactcgtg tctccatcca ctgatatgca
     3061 attaaacgac tcattgaccg caaatctaag agcctgttga agggcgctct tcgtgatcga
     3121 ggcgtctgtg gtgaaatagg ccagcattgt ggccatgttg ggatggatca ttcctgagcc
     3181 cttggccata cctccaattg tgatcaagcg accatcgatg gtgtcctgaa gtgcgacgga
     3241 tttcgggtgc agatcggtcg tcatgatcgc ctgagcagca tccaggcctc cgtggtcgct
     3301 gagctgagag agcagctgtg gtattccctt gatgattcga ttgaccggca ac
//
LOCUS       DATIPF010000387         5294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_342049, whole genome shotgun sequence.
ACCESSION   DATIPF010000387 DATIPF010000000
VERSION     DATIPF010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342049"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VLL06_09725"
     CDS             complement(<1..290)
                     /locus_tag="VLL06_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HSS31284.1"
                     /translation="MSEFVNVATVEEIPPGTGRTVEVQGVWIALFNVDGSFYAVDNTR
                     PHAGGPLGEGSLKGHIVECPWHGWRYDVQTGKRPENPEITVACCPVRIEGNQV"
     gene            457..1002
                     /locus_tag="VLL06_09730"
     CDS             457..1002
                     /locus_tag="VLL06_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HSS31285.1"
                     /translation="MEMTLLLNATYEPLRVVNWQKAIALLWQGKVEVLEVYDRDVHAV
                     SISIKLPSVMRLLKLVRLKDVHRAVKFSRINIFTRDSYACQYCRHKFRTEELTFDHVI
                     PIAKGGRKTWENIVTACWRCNNKKSGRTPEEAGMRLLKRPVKPRWNPIVTITIGIRNT
                     PDSWRDYFYWNMELDADPADT"
     gene            1214..1873
                     /locus_tag="VLL06_09735"
     CDS             1214..1873
                     /locus_tag="VLL06_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HSS31286.1"
                     /translation="MAANSGFPLSLDVRGYPVLVLGGDEEAAEKTQRLLEAGAKVIVV
                     APTLHDILKDLAASAKVLHRGRHFRDSDLESAILVMNMVRGDKEFSRALFAKAREKKV
                     LLWSVDQPEASTVTMPAVVACGHLRVAISTSGVAPALSGFMKEDMEKIFGEEFAAFVD
                     WLGELREHTKATEPDFEKRRALLREALDGFRLLGKVQYPKVWLDERAKLTVVPGVKGE
                     A"
     gene            1897..2247
                     /locus_tag="VLL06_09740"
     CDS             1897..2247
                     /locus_tag="VLL06_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HSS31287.1"
                     /translation="MVDGHNLAKEHDDNMVLLEFSMSPLGKGESVGKYVSRSLDIIDK
                     SGVDYRLNPMGTVLEGEWDDVVGVVKQCYERMKKDCGRISCTIKVDYRKGHTGRLQSK
                     VASVEKTLKRSIKH"
     gene            2889..5219
                     /locus_tag="VLL06_09745"
     CDS             2889..5219
                     /locus_tag="VLL06_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HSS31288.1"
                     /translation="MPDEVVLKTESGLELLPKDRTILEREFMEFRPFGLDENGHTIRD
                     LSGMSIKAVVVYLEKSQALAGGVSAGTQAVEELCQLLNQRMKDPVYHVTPKLLKNAWN
                     SYSYEFTAYLYEFCERLSGNPQLVFQGGMEKASSVMQVLARPFSLAQIYGMFPYFGNK
                     FASGSVKFRVVEVTPTSATLAMKFSGRTLRQFGPYRRRCAHLMCQAAQGIMAAVPVRV
                     HGLPPATLTDLSCMANDDEWCQWTIRWQAERQGTWRERIWQMAIGKRGERSPFVSHSH
                     EQPQAVGAKGTAGDREREIAQIRALTGATPIQRHVTWSISGALAGLMLAVGFRVMNPL
                     ASLGEVVLLGLTPMLVAGILINRNLRLESQQREALIQEQITFVESRHEELREAYLAQE
                     LTRVELRRKVVQLTALQRAGLLFSSTLDREALMQKVLEALTSDLQYDRAMISFFDPVR
                     QVVMDTRVLGVSPEIQAFVRTREIPVTDPMSPEGIVLLQGQPLLVGDVQAVWDRLHPL
                     NQRFALLTQTKAWVAVPLKVKDRVLGSLMVDRIQAHSLTQDDLELMMTVAHQVAIALD
                     NASAYEQIEDLNMGLEVKVRERTFELEQADRLRSQFLSLVSHELKTPLTSIKGFLQNL
                     LDGLTGPLNEKQQRYLSRMLDNSDRLIRMIEALLDRTRIQSGRLDLVPCEIDLGHCVA
                     DALEQLRLLAQAKQQTLEAVTPSAPLMVWADRDRLIQIVTNLAQNAVKFTPEGGSIIV
                     TVRQENQTLAGVSVRDTGPGIPAEFLDQIFDPFFRA"
BASE COUNT         1187 a         1321 c         1566 g         1220 t
ORIGIN      
        1 acttgatttc cttcgattcg gacgggacaa caggcgacgg taatttcagg attctccgga
       61 cgcttgccgg tttggacatc gtatcgccat ccgtgccacg gacattcgac gatgtgacct
      121 ttcagagagc cttctccgag cggtcctcct gcatgcggac gggtgttgtc gacggcatag
      181 aaggatccat cgacgttaaa cagcgcaatc cacactcctt ggacttcgac ggttcgtccg
      241 gttcctggcg gaatctcttc tactgttgcg acgttgacaa actcagacat ggcgcagatt
      301 ctttctcttg gccgagtggc cgacggaact ggtacattcc gcagactggc aatggcttga
      361 tttcgcccga ggcttatggc atagagaagg tacgcgttca atactacacc cgggccctct
      421 tgcgcaagga gcctcggttg ggaatgaggt tgttgcatgg aaatgaccct tctgctcaac
      481 gcgacgtacg aacccctgcg cgtggtcaat tggcaaaagg cgatcgcgct cctctggcaa
      541 ggcaaagtcg aagtcctcga ggtctacgac cgcgacgtcc atgcggtgtc aatctctatc
      601 aagcttccct ccgtcatgcg gcttctcaag ctggttcggc ttaaggatgt ccaccgggca
      661 gtcaagttct cccgcatcaa tatcttcacg cgagatagct atgcctgcca atactgtcgg
      721 cacaagttcc ggaccgaaga gctgacattc gaccatgtca ttcctatcgc gaagggtggg
      781 cgaaagacct gggaaaatat cgtgacggcg tgctggcgct gtaacaacaa aaaaagcggc
      841 cgtacgcctg aagaagccgg gatgcgactc ttgaagaggc cggtgaagcc gcgctggaat
      901 ccgatcgtga ctatcaccat cgggattcgg aacacaccag acagttggcg tgactacttc
      961 tattggaaca tggagttgga tgccgacccc gcagatacat aacgctcagt tgtgacattc
     1021 tcggtctgac tgtcgccgtt ttctctcaca taccctctcg ttccttaaac atccgtcaag
     1081 attcaactcg gcctgcctat acatctcgat ccaaccgaac catcaaaccc agtccgaaca
     1141 tgagacgggc ggtttacgct gcaaaacagg gtgttgtatg atacgtccgg ttatcgaatt
     1201 gggaggaact accatggctg caaattctgg ctttcccttg tctctggatg tcagagggta
     1261 cccggtgctt gtactgggcg gagacgaaga ggccgcggag aagacccagc gattgttaga
     1321 ggccggggcg aaagtgatcg ttgtggcgcc gacgctccac gacatactca aagatttggc
     1381 cgcatcagca aaagtcctgc accgtggtcg tcattttcgt gatagtgatc tggaaagcgc
     1441 gattctggtc atgaacatgg ttcgtgggga caaggaattt tcccgagcgc tatttgccaa
     1501 ggcgcgggag aagaaggtct tattgtggtc ggtggatcag ccggaagcgt cgacggtcac
     1561 gatgccggcg gtggtggcct gcggccattt gcgtgtggcc atcagcacaa gcggagtcgc
     1621 cccggctttg tcgggcttta tgaaggaaga tatggaaaag atcttcggtg aggaatttgc
     1681 tgcgtttgtc gattggctgg gagaactccg ggagcacacc aaggcgactg agccggattt
     1741 cgagaagcgg cgtgcattgt tgcgtgaagc gttggatggg ttccgcttgt tgggcaaggt
     1801 ccagtatccg aaggtctggt tggatgagcg tgcaaagctg actgtggtac caggcgtgaa
     1861 gggtgaagcg taagcggtga agcagcctca agacggatgg tggatggtca caatttagcg
     1921 aaggagcatg acgacaacat ggtcttattg gaattcagca tgtcgccatt aggcaagggc
     1981 gagagcgtgg gcaagtatgt gtctcgttcg ctcgacatta tcgataaaag cggagtggac
     2041 taccggttaa acccgatggg gacagtgctg gaaggggaat gggatgacgt agtgggcgtc
     2101 gtcaaacagt gttatgaacg gatgaaaaaa gattgcggcc gcatttcctg tacgatcaaa
     2161 gtcgactatc ggaaaggcca tacagggcgt ctacagagca aggtggcaag cgtcgagaag
     2221 acgctcaagc gatccataaa acattagcag gatgttgaaa acggtttcca gcgtcgttct
     2281 cagctcatca aaatcctcaa cgtacccgag agggtacgcc tccggttttg attcgcctgc
     2341 ggcctagctg gaaagccgtt ttgaacatcc tgcgaaactg ccagacatgc ggaccgtcga
     2401 atttctcgct gctctgaata ctctttcatc ttgctggcac actgaaacaa tctgaaacgc
     2461 gattatccta ttcattgaat cgccatcctc ttccaactcg agcctatctc ttagccgttc
     2521 gtgttcgtgt ttctaaatct ccttcggcaa tcctgctaag tctctccttc ggtcgtgcga
     2581 cggtgaatta tcctacgatc ataagtcaga atcgacttac aaatctttga aatgtaggaa
     2641 gcataataca cattatataa ttatttcata cattattgta ttcggatttg aaagtaatag
     2701 ggtgcctata tatactaagc gccgtaaaga ctgatgtgtg tctttgttga aggtgtgtca
     2761 tcgatgatat taacaagagt ccgaacaatc gatggtcgtg taacccaatc ttctccgtat
     2821 ctcgacagcc agcggatatt gccatccgca ggcctatgac ccccccacca tatccgatga
     2881 acagcaggat gccggatgag gttgtactca agacggagtc tggccttgag ctcttgccca
     2941 aggaccgcac gatcctcgag cgagagttta tggagtttcg cccgttcgga ttggacgaga
     3001 acggacacac gatcagagat ttgagcggga tgagtatcaa ggcggtcgtc gtctatctgg
     3061 aaaagtcgca ggctcttgcg ggaggagtct cagccggaac ccaggcggta gaggaactgt
     3121 gtcagctgtt aaatcagcgc atgaaagatc ctgtctatca cgtgaccccg aagctgttga
     3181 agaatgcgtg gaatagctac tcgtatgagt tcacggccta tttgtatgag ttctgcgagc
     3241 gcctgtccgg caatcctcaa ctggtgtttc agggtgggat ggagaaggcg tcttcggtca
     3301 tgcaggtgct cgccaggccc ttttcactgg cacagatcta cgggatgttt ccttacttcg
     3361 gcaacaagtt cgcctccggc tcggtcaagt ttcgagtggt cgaggtgacg ccgacctcgg
     3421 cgacgttggc gatgaaattt tccggccgca cgcttcggca gttcggcccc tatcgtaggc
     3481 ggtgtgctca tctcatgtgt caggctgctc aaggcattat ggctgcggta cctgttcgcg
     3541 tccatggcct gccaccggcc actctgacgg atctgtcctg catggcgaat gacgacgaat
     3601 ggtgtcaatg gaccattcga tggcaagcag agagacaggg tacatggcgc gagaggattt
     3661 ggcagatggc gataggaaag agaggggagc ggtcaccatt tgtgtcccat tcgcacgaac
     3721 agcctcaagc tgttggtgcc aaaggcactg ctggagatcg cgagagggag attgcgcaaa
     3781 tccgagcact gacgggtgcc acgccgatac aacggcatgt gacatggtcg atcagcggtg
     3841 cactagccgg tctgatgttg gccgtgggat ttcgcgtgat gaatcccttg gcgagcttgg
     3901 gcgaagtcgt tcttctcgga ttgacgccca tgctggtggc agggatattg atcaaccgca
     3961 atcttcgtct ggagagccaa caacgcgagg cgctgattca ggagcagatt acgttcgtgg
     4021 agtcacggca tgaggaattg cgggaagcgt acctggcgca agagctgacg cgcgtcgaac
     4081 tgcgcaggaa ggtggtccag ttgacggccc tccagagagc cggattgctg ttcagttcga
     4141 cgttggaccg agaggccttg atgcagaaag tgctggaggc tctgaccagc gacctccaat
     4201 acgaccgggc gatgatctcg ttcttcgacc cggttcgcca ggtcgtcatg gatacgcggg
     4261 tgctcggcgt gtcacctgag atccaggcgt ttgtccgcac gcgtgaaatt cccgtcaccg
     4321 atcccatgag cccggaaggc atcgtgctgt tacagggcca gccattgttg gttggggacg
     4381 tgcaggcggt atgggatcgc ctgcatccgt tgaaccaacg gttcgccctg ctgacgcaga
     4441 ccaaggcctg ggtcgccgtt ccgttgaagg tgaaagaccg ggtgttggga agcctgatgg
     4501 ttgatcggat acaggcgcac agcctgactc aggacgatct agagttgatg atgacggtgg
     4561 cccatcaagt cgcaatcgcc ctggataacg cctctgcgta tgaacagatt gaggacctga
     4621 acatgggact cgaagtcaaa gtgcgtgaac gcacgtttga gctggagcag gcggatcgct
     4681 tgcgttcgca attcctctcg cttgtctcgc acgagctcaa gaccccgctg acctcgatca
     4741 aggggttctt gcagaacctg ctcgatgggt tgacggggcc actcaatgaa aaacagcagc
     4801 ggtatttgtc acgcatgttg gacaattcag accggttaat ccggatgatt gaggccttgt
     4861 tggatcgtac gaggattcaa tccgggaggt tggatctcgt tccctgcgag attgacttgg
     4921 ggcattgtgt ggcggatgcg ctcgagcaat tgcgcctctt ggcccaggcc aaacagcaga
     4981 cattggaggc agtcactcca tctgctccat tgatggtgtg ggcggatcgc gatcgtctga
     5041 tccagatcgt gacaaatttg gcccagaatg ccgtgaagtt taccccggag ggcggcagca
     5101 ttatcgtgac ggtgaggcag gagaaccaga cactcgccgg ggtttccgtg cgcgataccg
     5161 ggccgggaat tccggcggag ttccttgatc agatattcga tcccttcttt cgagcctaac
     5221 aggtccggag cggaataaaa gggctgggtt taggcctgtc gatcgtgcgg acattggtgg
     5281 agttacaagg cgga
//
LOCUS       DATIPF010000388         3703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1227509, whole genome shotgun sequence.
ACCESSION   DATIPF010000388 DATIPF010000000
VERSION     DATIPF010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227509"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1779
                     /locus_tag="VLL06_09750"
     CDS             <1..1779
                     /locus_tag="VLL06_09750"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HSS31289.1"
                     /translation="GSQPSSVTGEVPNTNSAQAAADNRLYSIAWAPPAWLFQRGLDPL
                     QIFKQLGNLGTLSEVTVETSKLPDLASIDPEVCYLSWTMKLKTAKNKNVVEAVFEFVR
                     EDSQLTIIEESKGQESSVDSHPVALTADGSEGGPQPLGEILVESGIVSRQTLDHALAQ
                     QKRVGQILVEQKAATPQQVEQALQKQKQQESVAQSKKTDTASIRVDTDKIDKLINLVG
                     ELVITQSMLSDLGSRFEMRQMSVLLERMAQLERNTREIQERVMSIRMLPIGTAFSRFP
                     RLVRDLSAKAGKKIQLVLSGEETELDKTVIESIGDPLTHLVRNSADHGLEPPEERLDN
                     NKPELGTVRLNAFHEGGNICITVEDDGRGLNRDKILAKAIKQGLIAENEKLPDDQIWA
                     LIFKPGFSTAEKVTDVSGRGVGMDVVKRNIDALGGTVSIKTALGKGTTFTLKLPLTLA
                     IIEGMTVRVGKETYIVPLLSILESIQPKAGAIKTVVGKGELINVRDTYLPMMRMYEVF
                     SLQPEYTEPTKAILLILETEGERVAVMVDEILGQQQVVIKSMEQNFRKVAGVAGATIL
                     GDGTVGFILDVRGMLEIARRGMPVAV"
     gene            1895..2413
                     /locus_tag="VLL06_09755"
     CDS             1895..2413
                     /locus_tag="VLL06_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HSS31290.1"
                     /translation="MTAATLETATREVNQQIGLTTEGGQFLTFQLGDELYGVDILRVQ
                     EIKGYTAVTKIPNTPAHIKGVLNLRGTIVPIIELRTKFGMPTIEYTMFTVIVVVVIQE
                     KIMGLVVDAVSDVLNIDKKDIQPPPQFGAKVDVSFLRGIGKSGEKLVALLDMDRLLSD
                     SDLHEVSAVAAA"
     gene            2509..>3703
                     /locus_tag="VLL06_09760"
     CDS             2509..>3703
                     /locus_tag="VLL06_09760"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HSS31291.1"
                     /translation="MVTWFGNLKTMWKLMLGFAVMSAIMCLLGWVASQGLLSVRESLR
                     VVYEDYTIAGTDLASIGNNLNRTRTNDFMALDSNTKEEFEQVLSRDAEITESVKKPLE
                     AYAGTTLRISKTGRDEAKDLQKFRDIYASYVIATDSANELLKQSWNVPTKVEREALRD
                     KARAISTQNAGPKMEAAIVALNELISTVKEVAKDMNEEGRATAANALFMLVVGTAVAT
                     VIGLILGWAIARILSRNLTNVIAAAQSLGGGDLAARATVTTTDEVGQLAQAFNEMGAH
                     LQAKVMKEREQATKTEQFMVDAKRVLGHLAGGDLTDQMTTACEGDLEQIKVSLNSAIA
                     NLTTTLTTVREAAESVTTGAEEITKGNEDLSQRTSEQASSLEETSSAMEEMTSTVKQN
                     ADNAKQ"
BASE COUNT          933 a          962 c         1109 g          699 t
ORIGIN      
        1 ggaagccagc cgtccagcgt gacgggagag gtgccgaaca ccaactccgc tcaagcagcc
       61 gctgataacc gtctttactc aattgcctgg gcgccaccgg cttggttgtt ccaacgcgga
      121 ctcgacccgc tccagatctt caagcaactg gggaacctcg gtaccctgtc cgaggtcacc
      181 gtcgagacct ccaaacttcc cgatctggca agcatagacc cggaagtctg ctatctctcc
      241 tggaccatga agctgaaaac ggccaaaaac aaaaatgttg tggaggccgt gtttgaattt
      301 gttcgtgaag acagccagtt gaccatcatc gaagagagta aggggcagga gtcatccgtc
      361 gattctcacc ccgtagctct caccgctgat ggcagcgagg gtgggcccca acccctcgga
      421 gaaatccttg ttgaaagcgg cattgtctca cgccaaacgc tggaccacgc actggcacaa
      481 caaaaacgag tcggccagat actcgttgag caaaaagccg ccactccgca gcaggtcgag
      541 caggcactgc agaagcaaaa gcagcaggag tcggtcgccc agtcaaagaa aaccgatacc
      601 gcctcgatcc gggtcgatac cgacaagatc gacaaactca tcaatctcgt gggcgaactg
      661 gtcatcaccc agtcgatgtt gagcgatctg ggatcgcggt ttgagatgcg tcagatgtcg
      721 gtgttgctgg agcggatggc acaattggag cgcaacacgc gcgagattca ggaacgggtc
      781 atgagcatcc gcatgctgcc gatcggcact gcatttagcc ggtttccgag actggtccga
      841 gacctgtcag ccaaggcggg aaagaaaatt caacttgtcc tctcgggcga agagaccgaa
      901 ctggataaga ccgtgatcga atcgatcggc gatcccttaa cgcacctcgt cagaaactcg
      961 gccgatcacg ggttggaacc accggaagaa cgccttgata acaacaagcc cgaactcggc
     1021 accgttcgcc tcaatgcctt ccacgaagga ggcaacatct gcatcaccgt cgaagacgat
     1081 gggcgcggct tgaatcgcga caagatactt gccaaagcca tcaagcaagg attgatcgca
     1141 gaaaatgaga agctgccgga cgaccagatc tgggccttga ttttcaagcc cggtttctcg
     1201 acagcggaga aggtgaccga cgtttcgggt cgtggcgtcg ggatggatgt cgtgaaacgc
     1261 aatatcgatg cgttgggcgg caccgtcagt atcaagacgg cattggggaa agggacgacc
     1321 tttaccctca agctgccgct gaccttggct atcattgagg ggatgacggt ccgagtcggc
     1381 aaagaaacct acattgtgcc gctgctgtcg atcttggaat cgatccaacc gaaggccggt
     1441 gccatcaaga ccgtcgtcgg caagggcgaa ctcatcaacg tgcgcgacac atacttgccg
     1501 atgatgcgca tgtatgaggt attttccctg cagccagaat ataccgaacc gaccaaggct
     1561 atcttgttaa ttctggagac ggaaggtgag cgggtggcgg tcatggtgga cgagattctc
     1621 ggccagcagc aggtggtgat caagagcatg gagcagaatt tccgcaaagt cgccggtgtg
     1681 gcaggcgcga caatcttggg cgacgggacg gtcggtttta ttctggatgt ccggggcatg
     1741 ctggaaattg cacgtcgagg gatgccggtg gctgtgtaag gatcgttggt ttatctggtt
     1801 tgtcttgttc gtttggttgg tctggttcca aaccaaatca accagacaaa ccagatagac
     1861 cagataaacc agataaacga aaagggagga ctctatgact gcagccacgc ttgagaccgc
     1921 gacgcgagag gtcaatcagc aaatcgggct cacgacggag ggcggccaat ttcttacgtt
     1981 tcagctcggc gacgaactct atggcgtcga tattctgcgg gttcaggaga tcaaggggta
     2041 cacagccgtc accaaaattc ccaacacgcc ggcgcacatc aagggtgtgc tcaatctccg
     2101 cggcaccatc gtacctatca tcgaattgcg aaccaagttc ggcatgccta cgattgagta
     2161 tacgatgttc acagtgatcg tcgtcgtcgt gattcaggag aagattatgg ggctcgtcgt
     2221 ggatgccgtg tccgacgtac tcaatatcga caagaaagac atccagccgc ctccgcagtt
     2281 cggcgcaaaa gtcgacgtca gttttcttcg tgggatcgga aaatctggcg aaaagctcgt
     2341 ggcgctcctg gatatggatc gcttgttgtc cgacagcgat ttacacgaag tttcagcggt
     2401 cgcagctgca taagtgttcg tagggacatc ggcctggatc gaggtcttgg caaccatcga
     2461 gattatgata tcgggctaag gatacatcta gggagggaga aagagaacat ggtgacctgg
     2521 tttgggaatc tcaagacgat gtggaagctg atgttaggtt ttgccgtgat gagcgcgatc
     2581 atgtgtctgc tggggtgggt tgccagccag ggcctgctgt cagttcgcga gagcttgcga
     2641 gtcgtgtatg aagactacac cattgccgga acggaccttg ccagcatagg gaacaacctt
     2701 aatcggacgc gcaccaacga ctttatggca cttgattcca acacgaaaga ggaatttgaa
     2761 caggtgttgt cgcgcgatgc cgagatcaca gagtcagtca aaaagcccct tgaagcctat
     2821 gccggcacga ccctccggat atcgaaaacc ggcagagatg aagcgaagga tctccaaaag
     2881 tttcgtgaca tctatgcgtc gtacgtaatc gcaaccgaca gcgcgaatga gctcctgaag
     2941 caatcctgga atgtgccgac caaagtggaa cgagaggcgc tgcgcgacaa ggcccgcgcg
     3001 atctcgaccc aaaatgccgg gccaaaaatg gaagcggcca tcgtcgccct gaatgagttg
     3061 atcagcacgg tgaaagaggt cgccaaggac atgaatgagg aaggaagggc gacggcggcg
     3121 aatgcgctgt tcatgttagt tgtcggaacc gccgtggcaa ctgtgatcgg actcatcttg
     3181 ggttgggcga tcgcccggat cctgtcccgc aatctgacca acgtgatcgc agccgcgcag
     3241 agcctcggtg gcggcgacct cgcggcgcga gcgacggtga cgacgacgga tgaggtcggg
     3301 cagttggcgc aggccttcaa cgagatgggg gcgcacctcc aggcaaaggt gatgaaggaa
     3361 cgggagcaag cgacgaagac ggaacagttc atggtggacg cgaagcgagt gctcggacac
     3421 ctggcagggg gagatctgac ggaccagatg acgacggcct gtgagggcga ccttgagcag
     3481 atcaaggtca gtctgaacag cgcgatcgcc aatctgacga cgacactcac caccgtgcgt
     3541 gaagctgcgg agagcgtgac gacaggagca gaagaaatta ccaagggcaa cgaggatctc
     3601 tcgcagcgga ccagtgaaca ggcctcctcg ctggaagaaa catccagcgc aatggaagag
     3661 atgacctcca cggtgaagca gaacgccgac aatgccaagc agg
//
LOCUS       DATIPF010000389         4168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_782234, whole genome shotgun sequence.
ACCESSION   DATIPF010000389 DATIPF010000000
VERSION     DATIPF010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782234"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(453..776)
                     /locus_tag="VLL06_09765"
     CDS             complement(453..776)
                     /locus_tag="VLL06_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSS31292.1"
                     /translation="MVEDVRDENYKEFTGAAASIVAYGLATCEPCKTYDPILEETAAK
                     FPNVRVGKAKMHVPGRCREIKKSHTFETYPTTHFFSNGKLLLTREGVVEPDELAALIA
                     DYLIK"
     gene            complement(876..1346)
                     /locus_tag="VLL06_09770"
     CDS             complement(876..1346)
                     /locus_tag="VLL06_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSS31293.1"
                     /translation="MSDVAAEIKVGDVAPDFNLKDQDQKDVKLSDYRGKKNVVLCFYP
                     LDWSPVCQGENKCLTDDFPKFQSANAELFGISCDSFFSHKAWADSMDLKHRLLSDVHR
                     TTAKGYGLYFEPLNCSKRATVIVDKSGKVAYSKVQEIKVAREDKEILAALAKLS"
     gene            complement(1495..2250)
                     /locus_tag="VLL06_09775"
     CDS             complement(1495..2250)
                     /locus_tag="VLL06_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31294.1"
                     /translation="MRIRKKTPKPSARRPPLYFVGYRGTVPAPEELKTWYDLEYGGPL
                     TVRADEQATESWHAVHGPWTAHLVIPLPATHIAGLKEQLAWEHEHVGAVAPSLVPPRD
                     MPDTILFAARLARGLTLLTQGTAYDITTNQYVNPSDWQDRPLTQFILSDHIPIIQTDD
                     EQSRRNWCYTLGLSKFGVDELEMFLAPGLPDQPVRDLLGHTADELIRTGQSPKVGSHI
                     RVGLLEQSVTIANHRTAAPAGRMLSFREIRPLP"
     gene            complement(2265..4151)
                     /gene="typA"
                     /locus_tag="VLL06_09780"
     CDS             complement(2265..4151)
                     /gene="typA"
                     /locus_tag="VLL06_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HSS31295.1"
                     /translation="MDRTATSESPVTTHLEPSERRNDIRNIAIIAHVDHGKTTLVDAV
                     LRQTHVHRKIDDMGERIMDSMDQERERGITIRAKNASVTYKGVKINIVDTPGHADFGG
                     EVERTLRMVDGVLILIDAKEGPMPQTTFVLRKALALGHKAIVVINKIDRPDAVIDDVV
                     NRTFDLFVHLGATDEQLDFPIVYTAAIKGTATLDVNKPGTDITPLLDTVLEKIPAPAI
                     NAEAPLQILVLSLIQDLYKGKMGVGKIQSGSIARRQNVVVLGKNGAQFPGKVSDLAVY
                     SGLERTDMERAEAGEIVAVAGLDEVSIGDTIADSEHPVALTRVTVDEPTVQMTFSVNN
                     SPFAGREGKYLTSRHLRDRLFKELETNVSLRAQETDSADKFLVAGRGELHLGVLIEQM
                     RREGYELQVSQPEVIVHREGDKVLEPYEELTIQVPETYQGAVIEELGKRRGELRHMRL
                     IHSDAGPSEMHLEYHIPTRGIMGLKNLLVTKTRGTIIMHHVFVAYEPVEERELVVATH
                     GSLVAHEDGTSTGYAIFMTQERGTMFIGPVVEVYRGMVVGENSRDEDMDVNVCKEKHL
                     SNMRASGTDEALVLTPPREMGLEFALEYIGPDELVEVTPKSLRIRKRLLNPDDRRKAR
                     KSVK"
BASE COUNT          811 a         1126 c         1216 g         1015 t
ORIGIN      
        1 ttgtgatgga aaactctgct catcacagga acgaccgctg ttgttttggc tctgaagcgc
       61 ggtctggtcc ggagtcttcc atatgagtct gccgcctccg cttggttact gttaggagat
      121 cgcaaaggct atgccgaata gctggtgagg gggtcgatgt atttcgcagg gttatggagg
      181 ctcaatgggc cataaagccc cgcctggcgc cgatttgcgt cgatgacaga caaaagttgg
      241 gctgtacaaa aatgagctta aggatggtcc ttcgaaacct caatagaatg cagagatgtt
      301 cgtgaagagg agctactaac tagctgtata tacgtataat agcgcgagcg catggcatct
      361 tgaagaagag tcaaactggg gggtaaccaa tgcggtctaa gaatgaggat gaggagactc
      421 ttctcctctc agccgaagtc ggtgaggagg aactacttga tcaaatagtc cgcaatcagc
      481 gcggcgagtt cgtccggttc caccacccct tcacgggtca ggagcagttt gccattggag
      541 aaaaagtgcg tcgtggggta ggtctcgaag gtgtggctct tcttaatctc gcggcagcgg
      601 cccggcacgt gcatcttggc cttgccgact ctcacgttgg ggaacttggc ggccgtctct
      661 tccaggatcg ggtcgtaggt cttgcagggc tcacacgtcg ctaggccgta tgcgacgatc
      721 gaggccgccg cccctgtaaa ctccttatag ttttcgtctc tgacgtcttc gaccattccg
      781 gccatcttcg tcgtccaact ttcatcaggt cagaagtccg aaagtcagga agcccccatt
      841 gtggagactt tgactttcgg accctgcgac tgaaattagc tgagctttgc gagggctgcg
      901 agaatttcct tatcttcccg cgccaccttg atttcctgta ccttcgagta ggcgaccttg
      961 ccgctcttgt ccacgatgac ggttgcacgc tttgagcagt tcaacggctc aaagtagaga
     1021 ccgtagcctt ttgctgtggt ccgatgcacg tccgacagca gccgatgctt gagatccatg
     1081 gaatcggccc acgccttgtg cgaaaagaag ctgtcgcagc tgatgccgaa cagttccgca
     1141 ttggcggatt gaaatttcgg gaagtcgtcg gttaagcact tgttctcgcc ttgacagacg
     1201 gggctccagt ccaatggata gaagcagagc accacatttt tcttaccacg atagtcgctc
     1261 agtttcacat ccttctggtc ctggtccttc aagttgaaat ccggtgcaac gtcacccacc
     1321 ttaatttctg ctgctacatc gctcatctgc atccctcctt cattctattg gggttagtcc
     1381 ggcctctgac actgtcatcc atatttttta ccgtgcggct tgaaagcttg tcaacggtct
     1441 tgaaggccta gaagtggtct gactggtcca gaaacggccg ataagattga aagactatgg
     1501 gagcggccga atttctcgaa aactcagcat gcgcccggct ggtgcggcgg ttcggtggtt
     1561 ggcaatcgta acgctctgtt ccaggaggcc cacgcgaata tggcttccaa cttttggcga
     1621 ctgaccggtc ctgattaatt cgtccgctgt gtgtccgaga agatccctga caggctggtc
     1681 cgggagacca ggggcgagaa acatctccag ttcatccacg ccaaacttac tgaggccgag
     1741 ggtgtagcac cagttccggc ggctctgctc atcgtcggtc tgtatgatcg ggatgtgatc
     1801 gctgaggatg aattgggtga ggggacgatc ctgccaatcc gacgggttca cgtactggtt
     1861 ggtggtaatg tcgtaagccg tgccttgcgt gaggagtgtg aggccacgcg cgagtcgagc
     1921 ggcaaacaga atagtgtccg gcatatcgcg cggaggcaca agggatggag cgacagcacc
     1981 gacatgctca tgttcccatg ccagttgttc cttcagacct gcgatatggg tggccggaag
     2041 cgggatgacg aggtgagccg tccatggccc atggaccgca tgccaggatt ccgttgcctg
     2101 ctcgtcggcg cgcacggtca acggaccgcc gtattcgagg tcataccagg tcttgagctc
     2161 ttcgggtgcc ggcacagttc cccgatagcc gacgaagtag agtgggggac ggcgggcgga
     2221 aggtttgggg gttttcttcc tgatcctcat cgctgcaggg aaggctattt gacggactta
     2281 cgggctttgc ggcggtcatc cgggttgagg agccgtttgc ggatacgcag actttttggc
     2341 gtgacttcaa ccagttcatc cggaccgata tattcgagtg cgaattccag gcccatttcc
     2401 cgtggcggtg tgagaaccag cgcttcatcg gtacccgatg cccgcatgtt ggacaggtgc
     2461 ttttctttac agacgttgac gtccatgtct tcgtctcggc tgttttcacc gacgaccatc
     2521 ccgcgataga cctccacgac agggccgata aacatcgtgc cccgttcttg ggtcatgaag
     2581 atggcatagc cggtactggt tccgtcttcg tgtgcgacca gtgacccgtg tgtcgccacc
     2641 accagctcgc gctcctccac cggttcatat gccacgaaga catggtgcat gataatggtg
     2701 ccccgagtct tggtgaccaa gaggtttttg agtcccatga tgccgcgcgt agggatatgg
     2761 tattcgaggt gcatttcgct cggtccggca tcggaatgga tcagtcgcat gtggcgcaac
     2821 tcgcctcggc gtttgccgag ctcttcaatc acggcgcctt gataggtttc cggtacctga
     2881 atggtcagtt cctcataggg ttcgaggacc ttgtcccctt cccggtggac aatcacttca
     2941 ggttgagaga cttgcagttc gtatccttct cggcgcatct gttcgatcag gacgcccaga
     3001 tgcagctcgc cgcggccggc cacgaggaac ttatcggcac tgtctgtttc ttgtgcgcgt
     3061 aacgacacgt tggtttcaag ctctttgaag agccgatcgc gcaaatgacg ggaggtgagg
     3121 tacttgcctt cccgtccggc gaaggggcta ttgttcacgg agaaggtcat ttggacagtc
     3181 ggctcgtcga ccgtgaccct ggtcagcgcc actggatgct ccgagtcggc tatcgtgtcg
     3241 ccgatgctca cctcgtccag acccgccact gcgacgattt ctccggcttc ggcccgttcc
     3301 atgtccgtcc gttcgaggcc ggagtagacc gcgaggtccg acacttttcc agggaactgt
     3361 gcgccgttct ttccgagtac gaccacattc tgacgacgag cgatcgagcc ggattggatc
     3421 ttccccacac ccattttgcc tttgtaaagg tcttgaatga gagagagaac caggatctgg
     3481 agcggcgcct cggcgttgat agccggggcg ggaatctttt ccagcacggt gtcgaggagc
     3541 ggcgtaatgt ctgtgccagg cttattcacg tcgagcgtcg cagtgccctt gattgccgct
     3601 gtgtagacga ttggaaaatc gagttgctcg tcggtggcac cgagatggac gaacaagtcg
     3661 aaggtccgat tgaccacatc atcgatcact gcgtccggcc tatcaatttt gttaatgacc
     3721 acaatggcct tgtggccaag cgccaaggct ttccgcaaca cgaaggtcgt ttgcggcatg
     3781 ggaccctctt tcgcatcgat caggatcaag acgccgtcca ccatacgtag agttcgctcg
     3841 acttcaccgc cgaagtcggc gtggcccggt gtatcgacga tattgatctt cacgccctta
     3901 taggtcacgc tggcgttctt ggcccggatg gtgatcccgc gctcacgttc ctggtccatc
     3961 gagtccatga ttcgttcgcc catatcgtcg atcttgcgat ggacatgggt ctgtcggagt
     4021 acggcgtcga ccagtgtcgt cttgccgtgg tccacgtggg caatgatcgc gatattccgg
     4081 atatcgttcc ggcgttcgct gggctcgagg tgcgtggtca caggggattc tgatgtggcg
     4141 gtccgatcca tagatattgc tgcatgct
//
LOCUS       DATIPF010000390         4499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_960630, whole genome shotgun sequence.
ACCESSION   DATIPF010000390 DATIPF010000000
VERSION     DATIPF010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960630"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VLL06_09785"
     CDS             <1..1072
                     /locus_tag="VLL06_09785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HSS31296.1"
                     /translation="QNGQSVRRGDVLIELDPTLNRADHDRASNEYRAAKVEAARLRAL
                     IAGSPTFEAPSDGDAQYVLLQRQLLRDQLAEYQARTASAQHLIDQRKAGLDQTRENIR
                     RLEATVPMEVERADAYKRLLDHEAVTKMDFLQAEGQRIDKTQELAGQRKKLIQDQSAL
                     AEAEKNFRVLVSEFQQTKQMELSTTETKVASLAHDVTKAGQRADFQRLTTPIDGVVQQ
                     LVVHTVGGVVTPAQELLIVVPQDHPVEVEAQVENKDVGFVKADQVVEVKVETFTFTLY
                     GTIPGRVLSVSDDAAPIEKVGLVYPTRVSMDRATIQVEGKQVHLSPGMAVTVEIKTGQ
                     RRVIEYLMSPLLKSVQESLRER"
     gene            1158..2474
                     /locus_tag="VLL06_09790"
     CDS             1158..2474
                     /locus_tag="VLL06_09790"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSS31297.1"
                     /translation="MVSLVLLVIVLLVVIGNSVSWAKSEAIQPAPLALTLRGALDAAV
                     DNNPDVLLYKERIEAARGQVQTQLGAMLPNLSSNVRQTRQTQFRGTFGLPPVRSDPFS
                     IFDVRLNASQNLFSMSLLQRWRASRETLHATEFESDVRRFDTMASVALLYMEGLKAMG
                     MVKMHEANQLVMSELLGVVKQRQRGGVATGLDLARLEGQLANERQQIAAAQYDVQHAK
                     FSLVNLLGFAYEIPLSLTDEFQPEVHDVPAPQGAVEEALENRPEVRAQYTRVQAMELT
                     YSSITGERLPSLVAQGEYGLIGNRWNNTLDTYNMALLLQIPIFDGGQREGRIAESRSQ
                     LRQEALRMRSVLNQVKMEVHDAVASLAAAKEQVEIARIGMQAATKELELAKERYAVIT
                     TASHFELTNGLSAVARARENLVNALFQLNAARVNLARSTGSLNTLN"
     gene            complement(2564..3301)
                     /locus_tag="VLL06_09795"
     CDS             complement(2564..3301)
                     /locus_tag="VLL06_09795"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HSS31298.1"
                     /translation="MTKSRFQEELLRIMDRKHHWAWPAFADGTVTVDQLSRHFQQEYG
                     VYVRDFPVLLAHIYGQNPPAPVRRMLADNIYEEDTGGLSLGKSHPELFLAMMAGLGLP
                     ARDFDKVRLLPASRRYRAWLDRVSNNRDWVVGAAALTVFVEGSVKDRAELADPSKPKT
                     AEDIEAIVHRHPLVKYHGLSPDSMDLIRAHQMVEAGHRHDAYAMVVNYATTHTQQQAV
                     LGCLKKCLTLWQAYRDGVAKACGLKKT"
     gene            3371..3655
                     /locus_tag="VLL06_09800"
     CDS             3371..3655
                     /locus_tag="VLL06_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31299.1"
                     /translation="MNPITVQNPEDILSMLAEVSLRGSGFVTDCLLDYVLEEGFTEPI
                     YFNASGEDPNAYFKGQSPAWAVYQIREWKRVLTVSGGSGRERRVQITETP"
     gene            3749..3985
                     /locus_tag="VLL06_09805"
     CDS             3749..3985
                     /locus_tag="VLL06_09805"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HSS31300.1"
                     /translation="MPMIRLTEPQSMGELAMITSLLEGSGIAYIVQNEHVSSLYPGLP
                     ILNSSVMVDERDQVNAEMLLSRLRLAVRDVSSQP"
     gene            complement(3992..4450)
                     /locus_tag="VLL06_09810"
     CDS             complement(3992..4450)
                     /locus_tag="VLL06_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31301.1"
                     /translation="MHHPIAASQEPMNASLTDLEGPTPFIRRRPVRLIIYAGLVLFVL
                     VMVIWPLIAQLNDPDFHKHIAEHRVMVGMSKEQVLEAWGGPQTINTTFTKEGIRREEW
                     IFEDWENSAVVKHRYLYFEEGALIGGWFQGSGERLPMQSPSNPHTPKPRS"
BASE COUNT          990 a         1139 c         1412 g          958 t
ORIGIN      
        1 gcagaatgga cagtcggtca ggcgaggcga tgtgctgatc gagctggatc cgacgttgaa
       61 tcgcgccgat cacgatcggg cttcgaacga atatcgggca gccaaggtcg aggcggcgcg
      121 gctccgtgca cttattgccg gcagtcctac ctttgaggct ccttctgatg gagacgcgca
      181 gtatgtgctg ttgcagcgac agcttctcag agatcagctg gccgagtatc aggcacgaac
      241 cgcttccgcg caacacttga tcgaccagcg caaggcgggg cttgatcaga cgcgagagaa
      301 tattcgtcgg ctggaggcga cggtccctat ggaggttgaa cgggcggatg cctacaagcg
      361 gctgctcgac catgaggcag tcacgaagat ggattttctg caagccgagg gacagcggat
      421 cgacaaaacg caggaattgg ctgggcagcg caagaagctc atacaagatc aatccgcgct
      481 tgctgaggct gaaaaaaact ttcgggttct tgtctctgag ttccagcaga ccaaacaaat
      541 ggaattgtcg acgactgaga ccaaagtggc gtcgctggcg cacgacgtga ccaaagcggg
      601 acagcgggct gattttcaga ggctcacgac tccgattgac ggagtggtgc aacagttggt
      661 ggtccatacg gtgggcggcg tcgtcacgcc ggcgcaggaa ctgcttattg tggttccaca
      721 ggatcatccg gtggaggtcg aggcgcaggt cgaaaataaa gatgtggggt ttgtgaaggc
      781 ggaccaggtc gttgaggtca aggtcgagac gtttacattc accctctatg ggaccatacc
      841 tggccgggtc ctgagcgttt ccgacgatgc ggccccgatc gaaaaagtcg gcttggtcta
      901 cccgacgcga gtgagtatgg atcgagcgac gatccaggtg gagggcaagc aggtgcatct
      961 ctccccaggg atggcggtga cggtggagat taagactggc cagcggcgcg tcattgaata
     1021 tctgatgagt ccattattga agtcggtaca ggaaagtttg cgggagcgat agcggggacg
     1081 tgaagcgtga gggtaggcgt gacagggaag ggtgcaaacg ggtgactggt actgacgaaa
     1141 ccggcaggcg atctgtgatg gtctctctcg ttttgttggt gatcgtgctg cttgtggtca
     1201 taggcaacag cgtgagttgg gcgaagagcg aagcgataca gccggcgccg ctagcgctca
     1261 cgttacgagg cgcgttggac gccgcggtcg acaataatcc cgacgtgctc ctctataagg
     1321 aacggatcga agcggcgcgg ggacaggtac agacgcagtt gggcgcgatg ttgccgaatc
     1381 tgtcgtcgaa cgtccgccag acccggcaga ctcaatttcg gggtacattc ggtctccctc
     1441 cggtgcgctc cgatcccttc agcatcttcg atgtgagact caatgcatcg cagaacttgt
     1501 tcagcatgag tctcctccag cgctggcggg catcgcgcga aacgctccat gccacggagt
     1561 ttgaatcgga cgtccggagg ttcgatacga tggccagcgt ggcgctcctc tatatggagg
     1621 ggctgaaggc catgggtatg gtgaagatgc acgaagcgaa tcaattggtc atgagtgaat
     1681 tgctgggggt cgtcaagcaa cggcagcgtg ggggggtggc gaccgggctc gatctcgcca
     1741 gattggaagg ccagctggcg aatgagcggc aacagattgc ggcggcgcag tacgacgtcc
     1801 aacacgccaa gttcagtctt gtgaatctct tggggttcgc ctatgagatt ccgttgtcgc
     1861 tcaccgacga gtttcagccc gaggtccatg atgttcccgc cccccagggg gcggtggaag
     1921 aggccctcga gaatcgtcca gaagtgcgag ctcagtacac gcgcgtccag gcgatggaac
     1981 tcacctattc ctccatcaca ggggaacggt tgccgtcact tgtggcacaa ggggagtacg
     2041 ggctgatcgg aaatcggtgg aacaatacgc tcgacaccta caatatggcg ctgctcctcc
     2101 aaatcccgat ttttgatgga gggcagcgtg aaggccgaat tgccgaatct cgaagccaat
     2161 tgcgccagga agcgcttcgg atgcggtcgg tcctcaatca ggtgaagatg gaagtgcacg
     2221 atgccgtcgc gtccttggcg gcggcgaaag agcaggtcga gatcgctcga atcggcatgc
     2281 aggctgcgac aaaggaactg gaactggcaa aggaacgcta cgccgtgatc acaacggcga
     2341 gtcactttga actgaccaat ggactttctg ctgtggcccg ggccagagaa aacctcgtca
     2401 atgccctctt ccaactcaat gccgcgcggg tgaatcttgc cagatcgacg ggaagtctca
     2461 acacgttgaa ttgatcgttc ctccaccgta ttcgtcacat cgtcctcacg acccacacgt
     2521 ataggttttg gttgcaccga gagatagatc gccaacggag caattaggtc tttttcaatc
     2581 cacaggcctt ggcaactcca tctcgatatg cctgccagag cgtcaggcat ttcttgagac
     2641 agcccagtac ggcctgttgt tgggtgtgcg tggtcgcata gttcacgacc atggcgtagg
     2701 catcatggcg gtggccggct tcgaccattt ggtgtgcgcg aatgagatcc atggaatcgg
     2761 gggacaagcc gtggtatttc accagcggat ggcggtggac gatggcttcg atgtcttcgg
     2821 cggtcttggg cttggagggg tcggcgagtt ctgcgcgatc cttgacgctg ccttcgacga
     2881 aaacggtcaa ggccgctgct ccaacgaccc agtctcggtt attcgagaca cggtccagcc
     2941 aggcgcggta gcgtcgactg gctggtaaga ggcgcacctt gtcaaagtca cgcgccggca
     3001 atccaaggcc cgccatcatg gccaggaaca attccgggtg cgactttccc agcgagagcc
     3061 caccggtgtc ttcttcgtag atattatcgg cgagcatccg ccgaaccggg gcagggggat
     3121 tttgtccgta aatgtgggcg agcaacacag gaaagtcacg cacgtacacg ccgtactctt
     3181 gttgaaagtg gcgtgagagt tgatcgacgg tgaccgtccc atcggcgaat gccggccagg
     3241 cccaatgatg ctttcggtcc atgatacgca agagttcttc ttggaaccgg ctttttgtca
     3301 tgggacgtat catgtctgtt gccttcctgg tgagcggctc gttacaatcc gatagctggg
     3361 agatctatca atgaatccta ttacggttca aaatccagaa gacattttgt cgatgctcgc
     3421 ggaggtgtcc cttcgcgggt caggttttgt gacggactgt ctcctcgact atgtgttgga
     3481 agaaggcttc acggagccga tctatttcaa tgccagcggg gaagacccga atgcatattt
     3541 taaaggccaa tctcctgcct gggcggtcta tcagatccga gaatggaaac gtgtgctgac
     3601 ggtgtcgggc ggttccggta gagagcgccg cgtacaaatt actgaaacgc cgtagcctgt
     3661 ggatgagtgt aagagggatg ccgaaaaaaa gcaatgaaat gagggaggat cagctaccgg
     3721 tggccggttt gaccatggag ggctccacat gccgatgatc cgtctcactg agccgcagag
     3781 catgggtgaa ctggcgatga tcacgagtct gctggagggc agcggcattg cctatattgt
     3841 tcagaatgaa catgtgagta gcttataccc cggactccct attctcaatt cgagtgtcat
     3901 ggtcgacgag cgtgatcagg tcaacgcgga aatgctgctg agcagattgc ggttggcggt
     3961 gcgtgatgtc tcgtcgcagc cgtaattgtc tctagctccg aggttttggg gtatgcgggt
     4021 tcgacgggga ctgcatgggg agccgttcac ctgatccttg gaaccatccg ccgatgagcg
     4081 ccccttcttc aaaatagaga tagcggtgtt tcaccaccgc ggagttttcc caatcctcaa
     4141 aaatccattc ttcgcgacga atgccttctt tggtgaacgt cgtgttgatt gtttgtggcc
     4201 caccccacgc ctcgagcacc tgctctttcg acatgccgac catgacgcga tgttccgcga
     4261 tatgtttgtg aaagtctgga tcgttgagtt gtgcgatgag gggccagatc accatgacaa
     4321 gaacaaacag gaccagcccg gcatagatga tgagtctaac cggccggcgg cgaatgaaag
     4381 gagtcggtcc ttccagatca gtgagagagg cattcatcgg ttcttgacta gccgcaatgg
     4441 ggtgatgcat gacgcggtgc atcgtagcaa agtaaatcag agagagtcaa gagaagtca
//
LOCUS       DATIPF010000391         2961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_249320, whole genome shotgun sequence.
ACCESSION   DATIPF010000391 DATIPF010000000
VERSION     DATIPF010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249320"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VLL06_09815"
     CDS             <1..1176
                     /locus_tag="VLL06_09815"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HSS31302.1"
                     /translation="QVLISGAVRTPIGNLNGRLSDISATMLGGHVIRESIDRSHIDPS
                     LVDQVLMGTVLTAGVGQAPARQAARVAGLPESVSATTVGKVCGSGLEAVILGCQAIQA
                     GGAAVVVAGGMESMSQAPYLLSKQNAKQRLEWKVGELAESMIADGLWDVSSNQHMGVC
                     IEALIARYGFSREALDDFSVASYRNALASWQSGVFDMEIVNIPVMDGKDPMIFREDER
                     PGRFNEVKLRKLAPAFLPRGTVTAGNAAGINDGASSLVLISENQACALNISPVAKILA
                     YSRKGSAPDSFALAQVDAIKNILAQLSMTVDDIDLFEINEAFAAVPMAVMAELRLETE
                     RVNVHGGAIALGHPIGASGARILTTLIHALQRHKGRRGIASLCIGGGEGIALAVEIL"
     gene            1380..>2961
                     /locus_tag="VLL06_09820"
     CDS             1380..>2961
                     /locus_tag="VLL06_09820"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HSS31303.1"
                     /translation="MDDLTRQLSERFGFAKFRPGQEEVIRAVLAGRDAMAVMPTGLGK
                     SLCYQLPATLLPGLTLVISPLIALMQDQVASMRQRKIATAAFHSGLTGFEKSRVIQDL
                     QQRRLQLLYLAPERMQHEGFLQLLRSLWVSLLVVDEAHCISQWGHDFRPDYLKIGRLR
                     QELTNPPCLALTATATTRVQTDLCKRLSLRDPFRLVTGFRRANLALSVRLCQSRPEKL
                     ATLERLVRETENGTILVYCATRRAVEEAAEWLGQSQQSVGYYHAGLSDEERQLVHDDF
                     RRGTLRILAATNAFGMGIDKSDVRLVVHFDIPGSVEAYYQEVGRAGRDGRPAACVLLF
                     HERDLATQEYFIQQASQDAEGKDRAERMKTLLQEMLGYVSVSICRQLAILEYFSDDDE
                     RALGPCGLCDRCVAPMRQPTREISHDDAAVAKAILETVSWCMGRFGVSRIVEILRGSR
                     SKAMLAYGAEDCPTYGTYRRYSKPAMTGLVKTLIDSGHLQVEGTEYPTLDVTRRGQEI
                     LEGICAVVVKQAEVVQPDP"
BASE COUNT          611 a          759 c          926 g          665 t
ORIGIN      
        1 caggtcctta tctccggtgc agtccggaca cccatcggaa atctcaatgg acggctgagc
       61 gacatttcgg ccacgatgtt gggaggccat gtcattcgcg aatcgattga ccggtctcat
      121 atcgatcctt ctctggttga tcaggtgttg atgggaactg tgctgaccgc gggtgtcgga
      181 caagcccctg cccgccaagc ggccagggtc gcaggcctcc cggagtcggt ttcggccacg
      241 acagtgggaa aagtctgcgg ctccggattg gaggccgtca tccttggatg tcaagcgatt
      301 caagctggag gggctgcagt ggtggtggcg ggcggtatgg aaagcatgag ccaggccccg
      361 tatctactgt caaaacagaa cgcgaaacag agactagagt ggaaagtcgg cgagcttgcg
      421 gagagcatga ttgccgacgg tctttgggat gtgagcagca atcaacatat gggggtatgc
      481 atcgaggcgc tgatcgcgcg gtatgggttt tcgagagaag cgcttgatga tttcagtgtg
      541 gccagttata ggaacgcgtt ggcgtcgtgg cagtcaggag tgtttgacat ggagattgtc
      601 aacatcccgg taatggatgg aaaagatccc atgatatttc gtgaggatga acggcctggg
      661 cgttttaacg aggtgaaatt aaggaagctt gccccggcat ttcttccacg aggcaccgtc
      721 accgctggga atgccgcggg gattaacgat ggggccagct cgttggtgct gatttcagag
      781 aaccaagcct gtgcattgaa catctcgccc gtggcaaaga ttctcgccta ctcacggaaa
      841 ggatcagccc cagattcgtt tgccctcgcg caggtcgacg caatcaagaa tatcctagcg
      901 cagttatcaa tgacggtaga cgacatcgat ctgttcgaaa taaacgaagc atttgcggcg
      961 gttcccatgg ccgtgatggc agaactccga ttagaaaccg aacgtgttaa cgtgcatgga
     1021 ggcgccattg cactcggaca tccgattggc gcgagcggcg cgaggatctt aacgacattg
     1081 attcatgcat tgcaacggca caagggccgt cgcggtatcg cgagtctgtg tatcggaggc
     1141 ggagagggaa tcgcgttggc agtggagata ttatagtgat gccgaaatgg tcaggtccct
     1201 ccagagttcc ttcctcttat ccgcgtagcg gtcactggtg aagttgaaga ttacgctgag
     1261 ctgaaggtag aaagatgatc gctctacttc ggcgtgttca acttgcccgc gacgggtgac
     1321 tcgtctatac tcccacgcgc tcatggtctc caaccgtcca tcgtcgttcc aaactcaccg
     1381 tggatgatct gactcgacaa ctcagcgagc ggttcggttt tgcgaagttt cggcctggcc
     1441 aggaggaggt cattcgcgcc gtattggctg ggcgcgatgc gatggcggtc atgccgaccg
     1501 gcttgggcaa atcgctctgc tatcaattgc ctgcgacgct cttgcccggg ttgaccctgg
     1561 tgatttctcc cctcattgct ttgatgcagg atcaagtggc cagcatgagg cagcggaaga
     1621 tcgcgacggc agcctttcac tcgggcctta ccgggtttga aaaaagccga gtgattcagg
     1681 atctccagca gcggcggctc cagttgctct acctggcgcc ggaacggatg cagcatgagg
     1741 gatttctcca gctcttgcgc tcgctttggg tctccttgct ggtcgtcgac gaagcgcact
     1801 gtatttccca gtgggggcac gatttcagac ctgattatct caagattggt cgcctgcgac
     1861 aggaactcac gaatcctccc tgcctggctc tgactgccac agccacgacc cgcgttcaaa
     1921 cggatctgtg taaacggttg tcgctccgcg atccttttcg gttggtcaca ggtttccgtc
     1981 gtgccaatct cgcgttgtct gtccgtctct gccagtccag accggaaaaa ctggcgacgt
     2041 tggagcgtct ggttcgcgag acggagaatg gcaccatcct ggtctattgc gcaacgcgcc
     2101 gggcggtgga ggaggctgcg gaatggctgg gacagtctca gcagtcggtc ggctattatc
     2161 atgccggtct ctcggatgag gaacggcagc ttgtgcatga cgactttcgc cgggggacgc
     2221 tccggattct ggctgcgacg aatgcgttcg gcatgggcat cgataaatca gatgtccgat
     2281 tggtcgtcca tttcgacatc ccgggaagtg tggaggccta ttaccaggag gtcgggcggg
     2341 ctggtcggga cggtcggccc gccgcctgcg tgttgctgtt ccatgagcgg gatctggcca
     2401 cacaagaata tttcattcag caggcatcgc aagatgcaga aggtaaagac cgtgccgagc
     2461 ggatgaagac actccttcaa gagatgctgg gatatgtgtc ggtctcgata tgccggcagc
     2521 tcgcaatact cgagtacttc agcgatgacg atgaacgggc cttgggcccc tgcggacttt
     2581 gcgatcgctg tgttgcgccg atgcggcaac cgaccaggga aatttcccac gatgatgcgg
     2641 ctgtcgcgaa ggccatcttg gaaactgtgt cctggtgtat gggtcggttc ggcgtgagcc
     2701 gaattgtcga gattctccgc ggaagccgtt cgaaagcgat gctggcctat ggcgcggaag
     2761 actgtccgac ttacggaacg tatcgtaggt attcgaagcc ggccatgact ggtctggtaa
     2821 agacgttgat cgactcgggt catcttcagg tcgaaggcac ggaatatccc actctcgatg
     2881 tcacacgcag aggacaggag atcctggaag ggatctgcgc agtggtggtg aagcaggcag
     2941 aagtggttca gcccgatccg t
//
LOCUS       DATIPF010000392        14706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_518571, whole genome shotgun sequence.
ACCESSION   DATIPF010000392 DATIPF010000000
VERSION     DATIPF010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 14706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14706
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518571"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="VLL06_09825"
     CDS             <1..1545
                     /locus_tag="VLL06_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSS31304.1"
                     /translation="VLRHPRSMLAMTLATMGFSIYLYVVIPKGFFPQQDTGRLSGSIQ
                     ASQDISFQAMSQKLTEVAKIIQSDPAVDTVMGFSGGGGGSTTNTGRMFISLKPLAERK
                     LSADQVIARLRLKLATVPGAPTFLQAVQDLRIGGRVSSSQYQYTLQSVDLNALNTWAP
                     RVERQLRTLTDIVDVNSDQQDKGLQSLVVFDRGTASRLGLSPQLIDDTLYDAFGQRQV
                     SIMYTPLNQYHVVMEVAPQFWQSAAALNDIYVRSPAGMQVPLSAVARYEPTSTLLSVN
                     HQGQFPGVTLSFNMAPGTSLSEAVQAIEKSMRDIGLPGDIKGSFQGTAKAFQASLENQ
                     PLLILAALVTVYIVLGILYESYIHPLTILSTIPSAGVGALLALLLFKTELSMIALIGI
                     ILLIGIVKKNAIMMIDFALEAERKDEKPPEEAIYEACLMRFRPIMMTTMAALLGALPL
                     AIGMGVGSELRRPLGIAIVGGLLVSQLLTLYTTPVVYLFLDRVRLRFRSSRAIQFSPM
                     AKPTAR"
     gene            1771..2931
                     /locus_tag="VLL06_09830"
     CDS             1771..2931
                     /locus_tag="VLL06_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSS31305.1"
                     /translation="MMRDRLSRRYLRFWLVACLALTVWVGIPWTHAAALVTQEEVNSV
                     RVYKKLANATVLIASTYVSAHHITQASGKGLGSGVLIDEQGSIVTNAHVVDGAAKITV
                     TLHNGMRLPAELIGSDPQSDVALLHVTLPQGQHTPAHLGDSDKLEIGQKVVAIGHPFG
                     LGYAFSTGIVSGFGRVLEPKQDVIQERVIQTTTPFNPGNSGGPLVDSEDRVVGINSAI
                     LMGAQNIGFAIPINTVKSIVTELRRNGRVIRPWLGIKGKLVTDEVRSLIALPLVSGLL
                     ILDVEDGSPAETIGLRAGDLDVTIEGEPWILGGDILVAVNGQDVKSSEQYAKVLGQLK
                     AKQSIELTVFRDGLWHTMSVTLAERPSPRGPAQHPKIEVPPVVPQSLGFAPF"
     regulatory      3011..3218
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3666..4553
                     /locus_tag="VLL06_09835"
     CDS             3666..4553
                     /locus_tag="VLL06_09835"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HSS31306.1"
                     /translation="MNPVAYRPDGPAQENDWKDMFRQWDIIPFLSVHAMCLWAFQTGI
                     KPEWLLLAIGSYYLRMVGVTAGYHRYFAHRSYKTSRVFQFLLAFLAMTSAQKGVLWWA
                     AHHRHHHKYSDREEDFHSPLQRGFWFSHVGWTLSEKHMRADLSLIKDYAQYPEICFLN
                     RFYFIPPALYGLLIYMLWGFPGLIWGFFISTTALYHCTFFINSLTHMVGRVRYRSGDG
                     SRNSFILAILCCGEGWHNNHHHYQSTANQGWYWWEIDFSYYILVVLSWFGLVWDLRKP
                     PAHIKADTIAAESANPIGP"
     gene            4597..5739
                     /gene="cfa"
                     /locus_tag="VLL06_09840"
     CDS             4597..5739
                     /gene="cfa"
                     /locus_tag="VLL06_09840"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF008686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HSS31307.1"
                     /translation="MQLQSTRTVDPMKQKQVIQQLLDKAVVQIDGSQPGDILVHNDGL
                     YDRVLSEGSLGLGESYMDGWWDSVQLDEFFSKIHSVNLDESVVDKHLIFNAVKARVFN
                     MQNKRLSKRVAHEHYDLDTEFYEKMLGPRMQYTCAYWKRAQTLTEAQEHKLDLVCRKL
                     QLRKGERVLELGCGWGGFARHAATKYGCSVVAYNISSQQVAYARRWCAGLPVEIIQDD
                     YRTATGEFDKVAAIGLCEHVGYKNYRTLMETVHRTLKRRGLFLLHTIGNNKSVLTSDP
                     WFDKYIFPGGMLPSVAQLSAAQDGLFVLEDWHNFGPDYDRTLMAWHDNVNQHWDEFKS
                     KFDERFHRAWHYYLQSLAGAFRARKIHLWQVVMSKDGILGGYEPVR"
     gene            5745..7088
                     /locus_tag="VLL06_09845"
     CDS             5745..7088
                     /locus_tag="VLL06_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSS31308.1"
                     /translation="MITVEEYAGKRAHVLRSLTGTGVAEPLALAKSSSNLFRQRAQQR
                     VHQLDLRNFNHVLRINEQERYAEVEGLTTYEELVRETLAFNLMPAIVPQLKSITLGGA
                     IAGIGIESSSFKYGFVHETMQELEVLFPDGEVVVATRENEHRDLFFGFPNSYGTLGYA
                     LRVKIALVPVKPFVKLTHHRYTDLESYFQAMARLCREGSVDFVDGTMFHGQELYITTG
                     QFVDQAEWLSDYTYLQIYYQSIPTRTTDYLTTHDYIWRWDTDWFWCSKHFFLQYPLMR
                     RLWGKERLRSSVYWKLWKRFNASPVTTFVARAIEGRQESVIQDVEVPIEQAPRFAQFF
                     NEQIGIKPVWICPVAPFDPSASYALYPMKPQKLYVNFGFWDSVKTTHDDGHFNRMIET
                     KVSEFGGHKSLYSSSYYSKEEFGRLYSASEYQRLKTRYDPKGKLKHLYDKCVLKQ"
     gene            complement(7164..7610)
                     /locus_tag="VLL06_09850"
     CDS             complement(7164..7610)
                     /locus_tag="VLL06_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS31309.1"
                     /translation="MQRPKTGRPVQILLVEDNPGDVGLTLEALKEATLPNKLTIVKNG
                     ADALSLLRRQGQHAGAARPDLILLDLNLPKKDGRKVLAEIKGDDNLKRIPVVVLTTSE
                     DEQDVLESYNLHANCYIVKPLDLDRFITVVKSIEDFWLRIVVLPKN"
     gene            complement(7672..9441)
                     /locus_tag="VLL06_09855"
     CDS             complement(7672..9441)
                     /locus_tag="VLL06_09855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS31310.1"
                     /translation="MWSKTPLALDRPWWSGTVLPLALLLSFVIAIGVGLYTLTSLRAV
                     LVQERGADLGRTAARVTNTLDRVLFERFSDIQVFANDRILFEGKQDEKTQRLLQYKKL
                     YRYYSWVGVTDATGRIVVATNLVARPEAGVMTKTESRVALTQPDWFTMVRQTGRVDLE
                     GARVSRESEGMMAVGFSAPIYGPQGDFRGVVTTRVPIENLRAILEEEGKLPNGEDTHD
                     WLLLDREGAVIIEKSQASDMNSNLPKVELLSARRAAEDREKPGFVEELHQRRQIPVVT
                     GFARTQGYSNFPGFDWTVLVRLDREHVQAPINRLMWTAAGVGLLLITPLAGFGVWASW
                     RLVRDNRDLALSRKALEESIGELARSNADLQQFAYVASHDLKEPLRMVASYTQLLARR
                     YKGKLDSDADEFIRYAVDGANRMQWLINDLLAYSRVTSQDQVLEEVDCHGVLEEVLSD
                     LRVAIEESRAVVTHDPLPRVMADRGQLGQLFQNLIGNAIKFHGKEPPRVHVTAERKPD
                     EWAFSVRDNGVGLDPQYAERIFVIFQRLHDREEYPGTGIGLAICKKVVERHGGRIWVV
                     SQIGQGATFHFTLPIVGQSRHDS"
     gene            9938..11464
                     /locus_tag="VLL06_09860"
     CDS             9938..11464
                     /locus_tag="VLL06_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSS31311.1"
                     /translation="MDRFETNYRALLAVALVLNSQRDMHSLWEALTAEITKVIPWARA
                     SVTQYDREVDGFKFYVMATTMPQVVLKRDAVIPRVGSVMGWVYEHKIVHIRADLAQEQ
                     VFLEDTWYLQEGLGRMINLPLLVKDTCLGVLNIGSIESGAPEPEDLEFLAQVATQIAY
                     AIDHVQAYEQIDRLRDQLIKENVYLAEALRLTTNSDALVGKSLAFEHVMGLARDVAPT
                     PSTVFITGETGTGKELFAQMIHDWSPRHSKPMIRVNCAAFPAGLVESELFGHERGAFT
                     GAERSREGRFEQAHGGTIFLDEIGEMPLETQAKLLRVLQDGMVDRLGGNQPIRVDVRV
                     IAATNRNLPAAVKHGTFRADLFYRLNVFPISLPPLRNRPDDIPILARHFLKQYGIKFG
                     RSCKDINQESLERLIRYTWPGNVRELENVIERAMILSHDPVLRIDEHLLDLQDTSVAA
                     PPPADLKELERRHILQILTMTNWSIAGPDGAAVRLGIPPSTLRSRMKQFGMKRPTTKK
                     "
     gene            12562..13560
                     /locus_tag="VLL06_09865"
     CDS             12562..13560
                     /locus_tag="VLL06_09865"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSS31312.1"
                     /translation="MMSSVSPDWRDFAPTTWLRNPHFMTLFPLCWPRRPLLAGVPTES
                     RLFTTESHTQLLGFCHWQPHRTACRTVVLVHGLEGCSESHYMRGIAAKAYRAGFNVVR
                     MNQRTCGGTEHLTPTLYNSGLSQDYRTVVHELARMDRLDHIWLVGYSMGGNLVLKAAG
                     EAGSSEAALAGTIAVCPTIDPPRCVEALEQPRNWMYHRRFLVKLKARLRRKAALFPGK
                     WDLKELDGITRMREFDDRYTAPDGGYASEADYYNRTGAHHVLDSIAVSTLIITAQDDP
                     FIPYSMFTGPLIQRHPRIRMIAPRYGGHCGFFHRAQSGEDPYWAENRIMDFLQRRT"
     gene            13898..14170
                     /locus_tag="VLL06_09870"
     CDS             13898..14170
                     /locus_tag="VLL06_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31313.1"
                     /translation="MAQAHAPSVDSVICREVNRLVSCTLDELAQRLPPYSWAQVAAAV
                     DRLSRQGTLTVSRTRCFGYVVSVGPLPSTPQSLQERKCQALFGRIS"
     gene            14294..14698
                     /locus_tag="VLL06_09875"
     CDS             14294..14698
                     /locus_tag="VLL06_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS31314.1"
                     /translation="MAIRKNGARRCVMIVEQDAHFGIKLADWLAAHGYQPVLVRSVDA
                     AIEELREISPQVVFVGRSHPEPAAQNGISELLLLIQTLCPGVPVITIADQTSEDLTHD
                     GFRQGIHHVLVKPIEFTQISHVLRRELSSVTV"
BASE COUNT         3335 a         4153 c         3998 g         3220 t
ORIGIN      
        1 gtgctgcgac accctcgaag catgctcgcc atgacgttgg cgactatggg attcagcatc
       61 tacctctatg tcgtgatccc caaagggttc tttccccaac aagatacagg ccgcctgagc
      121 gggagtatcc aggcctcgca ggatatttcg tttcaggcca tgagccagaa gctgaccgag
      181 gtggcgaaga tcattcaaag cgatcccgcc gtggacacag tcatggggtt ctccggcggt
      241 ggcggcggga gcacaaccaa taccggccgc atgttcatct cattgaaacc gcttgcggag
      301 cggaagctca gcgcggacca ggtgattgcc agattgcgcc tcaagctcgc gacggtacca
      361 ggtgcaccaa cgtttctaca ggcggttcag gatttgcgga tcggcggacg tgtcagcagc
      421 tcacaatatc aatacacgct gcagagcgtc gatctgaatg cactcaatac ctgggcaccc
      481 agggtcgaac gccaattgcg tacgctgact gacattgtcg atgtgaacag cgatcagcag
      541 gataagggtc tgcaatcgtt ggtcgtattc gaccgtggca cagcctcgcg gctcggcctc
      601 agcccccagc tcatcgacga cacactctat gacgccttcg gccagcggca ggtttcgatc
      661 atgtataccc cgctgaacca ataccatgtc gtgatggaag tggcgccgca gttttggcag
      721 agcgccgctg cgctcaacga tatctatgta cggtcaccgg cgggcatgca ggtgccgttg
      781 agtgcagtgg cgcgctacga gcccacctca acgctgctgt ccgtgaacca ccaggggcaa
      841 tttcctggcg tcacgctgtc gttcaatatg gcgcctggca cctcgctcag cgaagccgta
      901 caggcgatcg agaaatctat gcgggacatc ggcctgccgg gcgatatcaa gggcagcttc
      961 caaggtacgg ccaaagcgtt ccaggcttcg ttggagaatc agccgctgct gatcctcgcg
     1021 gcgctggtga cggtctatat cgtattgggg atcctctatg agagctatat ccacccgctg
     1081 acgattctgt ccacgatacc gtccgcggga gtcggcgcat tgctcgcgct gctcctcttc
     1141 aagacggagc tcagcatgat cgcactgatc ggcatcatcc tcctgatcgg gattgtgaag
     1201 aagaacgcca ttatgatgat cgactttgcg ctggaagcgg agcggaagga tgagaagcca
     1261 ccggaagagg cgatctatga agcctgtctg atgcgctttc ggccgatcat gatgacaacc
     1321 atggcggcat tgctgggagc gctgcctttg gccatcggga tgggggtggg gtcggaactg
     1381 cggcgtccgc tcggcattgc gatcgtagga ggcctgctgg tcagccagct cctcacgctg
     1441 tacacgacac cggtcgtcta tctcttcctg gaccgtgtac ggctgcgatt ccgctcttca
     1501 cgggccatcc aattctctcc catggctaag cccacggcga gatgattcga cgagcgcgcc
     1561 agttgacatc gtcgcgataa ggaatgctgg cccaaccgta accttttcca ttctcagtac
     1621 gactagtgga tagatcaatt gaaagacccg atcggtcaag gatgtcggca tgcaatatca
     1681 tgcgcaatga cgctcgaggg actatcacaa taccggcaga ggtgatgggc gtgaagaaac
     1741 gatcgatgtg acgaatctca ggaggtatgt atgatgcgtg acagactttc tagacggtat
     1801 ctccgtttct ggttggtcgc ctgtctggca ctgacagtgt gggtgggaat cccctggact
     1861 cacgcagctg ccttagtcac acaggaagaa gtgaacagcg tacgggtcta caagaaattg
     1921 gcaaatgcca ccgtgctgat cgcctccacc tacgtcagtg cccatcacat cacgcaggca
     1981 tcgggaaaag gactcgggtc cggtgtgctg atcgatgagc agggctcgat cgtcaccaat
     2041 gcgcatgtcg tggacggggc agccaaaatc acggtgacgt tgcacaatgg catgcggctc
     2101 ccggcagagc tgatcggatc cgatccacaa tcggatgtcg cgctgctcca tgtgacactc
     2161 cctcaggggc agcatacccc tgctcacctt ggcgattcag acaaactgga gatcggtcag
     2221 aaagtcgtcg ccatcgggca cccttttgga ttaggttacg cgttttcaac cggcattgtg
     2281 agcggattcg gcagagtgct ggagcccaaa caggacgtga tccaggaacg agtgattcaa
     2341 actacgaccc cgttcaatcc cggcaatagt ggcgggccgc tggtggattc cgaggatcgc
     2401 gtcgtcggaa tcaactcggc gatcttgatg ggggcgcaga acattggatt cgccattccg
     2461 atcaataccg tcaagtctat cgtaaccgaa ctacggagga atggccgagt gattcgcccc
     2521 tggcttggca tcaaagggaa gcttgtgacc gacgaagtac ggagtctgat tgcgcttccc
     2581 ctcgtgagtg gattgctcat tctcgacgtg gaggatggga gcccggccga gacgattggt
     2641 ctccgtgccg gagacctgga tgtgacgatc gagggagaac cgtggatcct gggaggcgac
     2701 atcctcgtcg cagtcaatgg acaggatgtt aagtccagtg aacaatatgc caaggtgctt
     2761 gggcaactga aggccaagca gtcaatcgag ctcactgtct ttcgagacgg cctgtggcat
     2821 accatgagtg tgacgctggc agaacgtccc tcaccacgcg gtcctgcaca gcatcccaaa
     2881 atcgaggtgc ctcctgtggt tccgcagagt ctggggtttg ctccattcta ggacgatctg
     2941 cgggaggcga gaagagggac ataatcgtca ccgtcagtcg tcattctatt gacatcgttc
     3001 cttcagttag ataggctgcg ccagtgctgg tgctctgagt tttctcagag agaaaaaagg
     3061 gaactcggtg aaaaccgagg ctgccccgca gcggtaagcg agaacgattc cacaacacgc
     3121 actggccctt tcaagctgga aagctgtgga tagtaggagc gcggcaaacg ccgaaaagct
     3181 cgcaagcccg aagacctgcc agcacgtggc tctggagaga caaccggagc cggtacgaga
     3241 aggaagcctc gagggaggtc agtcggtctg agctggttcg gatttcagtc ctcacttccg
     3301 ttctaggttc cgacccaccc ttccacatgg ttgtttagtc acccgtgggg atataagtcg
     3361 gtacgagatt ccctcgcgca tcagacgcat ctcgactggc attggctcct cgcggtcttg
     3421 cgcaaagttc gatccttgtg cgcaaccgat gacggagggg gaagttcatg ccaggcgtac
     3481 ctgtcgcaag aatcaaccgc ctcataaccc agccagacat cactccgacc caagacgccg
     3541 tcagacgagc aggctcacaa cgattcgtca ttcgattcga acatggaaat gagagcctgg
     3601 gtcctcatcg tgggtaacgt agcgacagcc tgcatcgcgc atccggaagg aacgagaatt
     3661 tctgcatgaa ccctgtcgcg tatcgccccg atggtcccgc ccaagaaaac gactggaaag
     3721 acatgttccg gcaatgggac atcattccct ttctgagcgt ccatgccatg tgcctgtggg
     3781 cgtttcagac cggaattaaa ccggaatggc tgctactcgc catcggcagc tattatctga
     3841 ggatggtcgg cgtgacggcg ggctaccacc gctatttcgc tcaccgctca tacaagacta
     3901 gtcgggtgtt tcaattcctg ttggcctttc ttgccatgac ctccgcgcag aagggggtgc
     3961 tttggtgggc cgcccaccac cggcaccatc acaaatattc agaccgggaa gaagacttcc
     4021 attcgcccct gcagcgggga ttctggttct cccatgtcgg gtggaccctg tccgaaaagc
     4081 atatgcgtgc ggacttgagc ctcatcaaag attacgccca atatcctgaa atctgcttcc
     4141 tcaaccggtt ttacttcatc ccgccggctc tctacggcct gctgatctat atgctctggg
     4201 gtttcccagg cttgatctgg ggctttttta tttccacgac cgcgctgtat cattgcacgt
     4261 tctttatcaa ttcgctgaca cacatggtcg gtcgagtcag gtaccgatcc ggcgacggga
     4321 gtcgaaacag ttttattctc gcaatcctct gctgcggaga agggtggcac aataaccacc
     4381 atcattatca atcgacggct aatcagggct ggtactggtg ggagatagac ttttcctact
     4441 acatattagt cgtgctgtca tggtttgggc ttgtatggga tttgagaaaa cctcctgcac
     4501 acatcaaagc cgacaccatc gccgccgaga gcgcgaaccc gataggaccg tagttcctcc
     4561 tccggcccct gatcaggtca caacttcaga acacagatcc aactccagtc aacaaggacg
     4621 gtcgatccga tgaaacagaa acaggtcatt caacaattgc tcgataaagc ggtggtgcaa
     4681 atcgatggca gccagcccgg ggatatcctg gtgcacaatg acggactcta cgatcgtgtc
     4741 ctgagcgagg gctcgttggg actgggtgag tcatacatgg acggctggtg ggattcggtc
     4801 cagctcgatg agttcttttc aaagattcat agcgtcaatc tcgacgagtc ggtcgtcgac
     4861 aagcatctga ttttcaatgc ggtcaaagcg cgcgtcttca atatgcagaa caagcggctc
     4921 tccaagagag tggcgcacga acactacgat ctggatacgg aattctatga gaaaatgtta
     4981 ggcccgagga tgcaatacac ctgtgcctac tggaaacggg cgcagacgct gaccgaagca
     5041 caggagcaca agctcgattt ggtctgccgg aaattgcaac tccgcaaagg cgaacgggtc
     5101 ctcgaactcg gatgcgggtg gggtggcttt gcccgccatg cggcgaccaa gtacggttgt
     5161 tcagtggtgg catacaatat ttcttcgcag caggtcgcct acgcgcgacg ctggtgtgcg
     5221 ggccttcctg tggaaatcat tcaggacgac taccggacag ccacagggga attcgacaag
     5281 gtggcggcaa tcggactgtg cgagcatgtg ggctataaga attaccggac gctcatggaa
     5341 accgttcacc gtacgttgaa acggcgcggc ctttttttac tccatacgat cggcaacaat
     5401 aaatcggtgt tgacgtccga tccgtggttc gacaagtata ttttcccggg cggtatgctt
     5461 ccctcggtcg cgcaactcag cgcggcgcag gacgggctct tcgtgttgga agactggcac
     5521 aacttcggtc ccgattatga ccggacgctc atggcctggc acgacaatgt gaatcagcac
     5581 tgggatgaat tcaagtcgaa gttcgacgag cgcttccatc gggcctggca ctattatctg
     5641 cagtctctcg ccggagcatt ccgcgccagg aaaatccatc tgtggcaggt ggtcatgtcc
     5701 aaagatggca tcctgggcgg ctatgagccg gtgcgttaga ccacatgatc actgttgaag
     5761 agtacgccgg caagcgagcc cacgtgctca ggtccctgac tgggaccggc gtggcggaac
     5821 cgctggcgct tgcgaaatct tcctccaatc tgtttcgtca gcgcgcgcag cagcgagtgc
     5881 accaactgga cctgcgcaat ttcaaccatg tgctccgcat caatgaacag gaacggtatg
     5941 cggaagtcga aggcctgacg acctatgagg aactggttcg ggaaacactc gcctttaatt
     6001 tgatgccggc catcgtgcct cagctcaaat ccatcaccct cggcggggcc attgccggca
     6061 ttggaatcga atcgtcgtcc ttcaaatatg gctttgtaca tgaaacaatg caggaactcg
     6121 aggtactgtt tcccgacgga gaggtcgtgg tggccacccg tgagaatgaa caccgagact
     6181 tatttttcgg attccccaac tcctacggca cactcggcta tgccctgcgg gttaagatcg
     6241 cactcgtccc cgtcaagccg ttcgtcaagc tcacgcacca caggtatacg gacctggaat
     6301 cctactttca ggcgatggcc cgcctatgca gagaggggag cgtggatttc gttgacggga
     6361 cgatgtttca cggccaggaa ctctacatca cgaccggcca attcgttgac caggcagaat
     6421 ggctcagcga ctacacctat ctacagatct attaccaatc gatcccaacc agaaccacgg
     6481 actatctgac gactcacgac tacatttggc gttgggatac cgattggttc tggtgctcca
     6541 aacatttttt tctgcaatat ccattgatgc ggcgtctgtg ggggaaagaa cggctgcgtt
     6601 catcggtgta ttggaagctc tggaaacggt ttaatgcttc ccccgtcacc actttcgtgg
     6661 ccagagcgat cgaaggacgg caagagtctg tcatccagga cgtcgaagtg ccgatcgaac
     6721 aggccccccg ctttgcgcag tttttcaatg agcagatcgg aatcaaaccg gtctggattt
     6781 gccctgtagc cccgtttgat ccctcggcgt cctatgcgct gtatccgatg aagcctcaga
     6841 agctctacgt caattttgga ttttgggact cggtgaagac cacgcatgac gacggccact
     6901 tcaatagaat gatagaaacc aaggtgagtg agttcggcgg gcacaaatcg ctctattcaa
     6961 gctcctatta ctcaaaagag gaatttggac ggttgtacag cgcaagcgaa taccaacgac
     7021 tgaaaaccag atatgacccc aaggggaaac tcaaacatct ctacgataag tgcgtgctca
     7081 aacagtaacg cctattacga tcgtcgcctg gcttttttcc tatgtaggcc ttgcagacga
     7141 atgactcact cggcctcgct catttaattc ttcggcagca ctacgatcct gagccagaaa
     7201 tcctcgatcg atttcaccac cgtgatgaac cgatccaaat ccagcggctt cacaatgtag
     7261 caattcgcgt ggagattgta gctctccaac acgtcttgtt catcttctga agtcgtgagc
     7321 acgaccacgg ggatgcgttt caggttgtca tcacccttga tctcagcgag cacctttcga
     7381 ccatctttct tgggaaggtt aaggtcaagc aggatgagat ccgggcgtgc agcgcctgcg
     7441 tgctgccctt gccggcgcag caaggacaag gcatctgcgc cattcttgac aatcgtgagc
     7501 ttgttgggca gcgtggcctc tttgagggcc tcgagcgtca gaccgacatc gccgggatta
     7561 tcttcgacta agagtatctg cactggtctg ccggtcttgg gtctttgcat gcgccgtctc
     7621 cttgcgtgaa aatcatacgt gactcatgat ccctacggat cgtttcaacg attacgaatc
     7681 atgccggctc tggccgacga tcggcagagt gaaatgaaag gttgctccct gacctatctg
     7741 tgataccacc caaattcgtc cgccatggcg ttcaacaacc tttttgcata tcgccagacc
     7801 gatgccggtg ccgggatatt cttctcgatc gtgcaggcgc tgaaaaatga caaagattcg
     7861 ctcagcatac tgagggtcca gcccgacgcc attgtcgcgg accgagaacg cccattcatc
     7921 gggcttacgc tctgccgtca catgaacccg gggcggttcc ttgccgtgga acttgatggc
     7981 attaccgatc aggttctgaa agagctgccc cagctgtccc ctgtcggcca tcactctggg
     8041 aagagggtcg tgggtcacga ctgctcggct ctcttcgatc gcaacccgca aatcgctcag
     8101 cacctcttcc agaacgccat gacagtccac ctcttctaac acttggtcct gtgaagtcac
     8161 acgtgagtag gccaagaggt cattgatcag ccattgcatg cggttggccc catcgacggc
     8221 atacctgatg aactcatcgg catcggaatc aagcttgccc ttataacgcc tcgccaagag
     8281 ctgcgtgtaa ctggcgacca tccgcagcgg ctccttcaag tcgtgcgagg ccacgtaggc
     8341 aaactgctgc aaatcggcat tggagcgagc cagctcgccg atcgactcct ctagggcttt
     8401 ccgcgacaga gccagatcgc ggttatctcg aaccagtctc catgacgccc acacgccgaa
     8461 accggccaat ggcgtgatga gcaggagccc cacgcctgcg gctgtccaca tgagccgatt
     8521 gatcggagcc tgaacatgtt cacggtccaa ccgaaccaac accgtccagt cgaatcctgg
     8581 aaagttgctg tacccttgtg tcctggcaaa gccggtcacc acaggaatct gccggcgctg
     8641 gtgtagttcc tctacaaagc ccggtttctc acgatcctcg gcggctcttc ttgcggaaag
     8701 gagctccacc ttcggcagat tgctgttcat atcgctcgct tggctcttct cgatgatcac
     8761 cgctccctca cgatccagca gcaaccagtc gtgagtgtct tctccattgg ggagtttccc
     8821 ctcctcctca agaatggccc gtaggttctc aatcggaacc cgggtcgtca ccactccgcg
     8881 aaaatctcct tgtggcccat agatcggagc cgagaacccc accgccatca tgccctctga
     8941 ctctcgtgaa acacgggctc cctccagatc gacccgccct gtttgtctca ccatcgtaaa
     9001 ccaatccggc tgcgtgagag ccacgcgact ctctgtcttc gtcatcacac ccgcctcagg
     9061 cctggcaact aagttggtcg ccacaacgat tcgcccagtc gcatcggtca ctcctaccca
     9121 ggagtagtac cgatacagtt tcttatattg cagtaacctc tgggtcttct catcctgttt
     9181 gccctcgaac aatattctgt cattcgcgaa cacctggata tcactgaacc gctcaaacaa
     9241 cacgcgatcg agtgtatttg tgactctggc cgccgtcctg ccgaggtccg cccctctctc
     9301 ctggaccagc acagctctca aagacgttaa tgtatagagc cccaccccta tcgcgattac
     9361 aaaagacagg agcaatgcca ggggaagcac ggttcctgac caccagggtc tgtcaagcgc
     9421 cagtggggtc ttactccaca aatgttctac ttcctctttc tctatctcca gaacatgtca
     9481 ccctgcttcc tgagatcgtt ggagtgaaac ctgcaacggt tattggatga gacctatcgc
     9541 gatagacata tggcggagac gagataggcg tccctatctg aacgtgcttc ccggaagggg
     9601 gatgcaaaga gaatgcgggt aacatacaaa acagatcgat tgatgggacg cacgcaatat
     9661 ctgcagacct gttctccgtc aacagccaaa ggaagggtca tataccggat tgagcggaac
     9721 tgatgccaag atagcaggct tcaagattcg agacaatagt cagcctttcg aatgtaggag
     9781 tgagaaccat ctcggggcga cagatcagaa ctgagaaatt tttgttcacg catgcagagc
     9841 gacttctctc tattggagca ttcagctttc tgataggatg ttgcgagatg aacgcagctc
     9901 ggtgattcga ggtaccacgg taggatgagt gatgcctatg gaccggtttg aaacaaacta
     9961 tcgagccctc ttggcagtgg cccttgtgct caattcgcag cgggacatgc acagtctatg
    10021 ggaagccctg acagccgaga tcacgaaggt cattccgtgg gcacgcgcat ctgtcaccca
    10081 gtacgatcgt gaggtcgatg ggttcaagtt ttatgtcatg gctacgacga tgccgcaggt
    10141 ggtgttgaaa cgcgatgcgg tgattccacg agtcggaagc gtgatgggtt gggtctatga
    10201 gcataaaatt gtccacatcc gtgccgacct ggcgcaggaa caggtcttcc ttgaagacac
    10261 atggtacctg caagaaggcc tcggacgcat gatcaatctg ccccttctcg tgaaagacac
    10321 gtgcctcggt gttctcaaca tcggcagtat cgagtcgggc gctcccgagc ctgaggatct
    10381 cgagttcttg gcgcaggtcg cgacgcagat cgcctatgcg atcgatcatg tccaagcgta
    10441 cgaacagatc gatcgactcc gagaccaatt gatcaaagaa aacgtgtacc ttgctgaggc
    10501 actgaggctc acgacgaaca gcgacgcatt ggtaggaaag agcctggcct ttgagcatgt
    10561 gatgggcctt gcacgcgacg tggcccccac tccgtcgacc gtgttcatta cgggtgaaac
    10621 gggcacgggc aaggagctgt tcgcgcaaat gatccatgat tggagcccgc ggcactcgaa
    10681 acccatgatt cgtgtcaatt gcgccgcctt cccggcaggg ctggtcgaga gtgaactgtt
    10741 tggccacgaa cgaggcgcat tcaccggggc cgagcgttcc cgcgaaggca gatttgaaca
    10801 ggcccatggg ggaaccatct tcttggatga aattggtgag atgcccttgg agacacaagc
    10861 caagctgctg cgagtcttac aagatggcat ggtggaccgt ctgggcggga accagcccat
    10921 tcgtgttgat gtgcgtgtga tcgccgccac caaccgcaac ttgccggccg ccgtcaaaca
    10981 cggcaccttt cgcgccgatc tcttttatcg tctgaatgtg tttcctatca gcttgcctcc
    11041 tctccggaat cggcccgacg atattcccat attggctcga catttcctga aacagtacgg
    11101 cataaaattc ggccgctcat gcaaggatat taaccaggaa tctctcgaac ggttgattcg
    11161 ctatacctgg cccggcaacg ttcgcgaact ggaaaatgtg attgaacgag cgatgattct
    11221 ctctcatgac ccggtgttgc ggatcgacga gcatctgttg gatttacaag acacctccgt
    11281 cgctgccccg ccaccggccg acctcaaaga actcgaacgc cggcatattt tacagatcct
    11341 caccatgaca aattggtcca tcgcaggccc cgatggcgcg gcggtgcgct tgggaatccc
    11401 ccccagcaca ttgcgcagcc ggatgaagca gttcgggatg aagcgaccga caaccaagaa
    11461 gtagcccgag tcacaccacc caactgcggc agataatctg cctgcatcta catcgttccc
    11521 atcagcatgc ctcctcatcg tctgccggca tctgccagcg actcattctg ttcgtcacaa
    11581 gaacgaaaac atccggcaca atgatctcac cactcccttt cggaaggagc cgcacgcgag
    11641 cagtcgcgcg ctgggctcgc gagaagccgc gatccatcgc ggattcctcc atgttctctt
    11701 cagtttatct gggggacaga ctattaaatg cgcattgttt caagtagttg cgtgtattct
    11761 ctttaaaaat tcatagacgg cacgacctgt gcatgaactg gctgttactc gaacgattca
    11821 acccatcact ctagatgagg aggaactgta cgggtttcct gaaaggagga actttatgag
    11881 aaccgcaatg ttgatcaatg gcgtgatgta cgtctccttg attggcgttt tggccatggc
    11941 atggggatgg ttgcacagaa gtgattctta attgagaagt ggataaagat tgtacatgct
    12001 cctggaagga ggaacatcac gatgactact gcgttgatta atggcctatt actttcggga
    12061 atcgcgatcg ctgtgtttgt cggaaccatg gcgatgctca tggggacggc tacagagaag
    12121 taattgttgg atgggaatcg atggcgcgag ccagcgaatc ccgcctggat caagacagat
    12181 tccagatggt gctctgttcc tcgaatagcc ccgtatcgtc ggagcggtaa gaatagaacg
    12241 tcgcttgtaa ggacgccacc tcgaactcct catgccgtgc acttctgtat agaagcgcac
    12301 ggcatgcaca cctccagtag tctttccaag ccagaccgcc ttcgttcgta ctggcggctc
    12361 attgtcgccg acagataagc cccttgtgac tggtccggct tttgggtagt ctcttcttca
    12421 tcacaggtcc tgcgcctgcc gaatcaggct tctccgagaa agacgagcac gacgacaccg
    12481 agccacaaag ggtagaaggc actcccctcc cggtgtgtcg ccttctgcga ttcatactgc
    12541 gaagccatgg aacgtctccc tatgatgagc tcagtatcac cagactggcg agattttgcg
    12601 ccgaccacgt ggctccgcaa tccacatttc atgaccctgt ttccactctg ttggccacgc
    12661 cgaccactgc ttgccggagt accgaccgag tcgcggcttt tcaccaccga gtctcacacc
    12721 cagctactcg gcttctgtca ctggcagcca caccgcacgg catgccgaac cgtggtcctc
    12781 gtgcacggcc ttgaaggctg cagcgagtct cactatatgc gtggcattgc cgcgaaagcc
    12841 tatcgtgcgg gattcaatgt cgtccggatg aaccagcgga cgtgcggcgg aaccgaacac
    12901 cttacgccga cgctctacaa cagcggcttg agtcaggact atcgaaccgt cgttcatgaa
    12961 ttggcacgta tggacaggct cgatcacatc tggcttgtcg gttattcaat gggaggaaat
    13021 cttgtcctga aagcagcggg agaagccggc tcgtcagaag ccgcattggc agggacgatt
    13081 gcggtctgtc cgacgatcga ccccccgcga tgcgtcgagg ccttggaaca gccgcgcaac
    13141 tggatgtacc acagacggtt tctcgtgaaa ttgaaggcac gactgcgtcg aaaagccgcg
    13201 ctcttccccg gcaagtggga tctcaaggaa ctcgatggta tcaccaggat gagagagttc
    13261 gacgaccgct ataccgcccc ggacggaggc tatgccagtg aagccgacta ctacaatcgt
    13321 acaggcgcac accatgttct cgattccatc gccgtctcca ccctcattat cacagcacag
    13381 gatgacccct tcattcccta ctccatgttt acgggaccgc tgattcagcg ccaccctcga
    13441 attcgaatga tcgcgccgcg ctacggcgga cattgcggat tctttcaccg ggcacagagt
    13501 ggagaagatc cctactgggc agagaatcgg attatggact tcttgcaacg acgaacgtga
    13561 ggaacacatg ggaacggagc atggctggcc gccaagcata tccgatacat acgggatcat
    13621 tcctagaaat gctgatctgc tcagaacgtc tggccctggg aataaaaagc agcacctccc
    13681 ttttgcctgt cgcacaatcc acgacagtgt tggttgagtc gattcgacaa gagaatccta
    13741 gttttgtcga tagggcttca accacgactc agctataacc tcgcacagat gagcaacgaa
    13801 cagatctcac gactgtgagg gcacttttct caacacctcc acgaaactac caggacattc
    13861 aatcacgagc ccattcatct ccaaggagga gggtacaatg gcacaggcgc acgcccccag
    13921 cgttgactcc gtcatttgcc gcgaagtgaa cagactcgtg tcctgtaccc tcgatgagct
    13981 ggctcagcga ttaccgccct attcatgggc gcaagtggct gcagcagtgg atcggctcag
    14041 ccggcaagga acactgacgg tcagccgcac gcgctgtttt ggctacgtcg tctcggtcgg
    14101 gccacttcct tcaactcccc agtctttgca agaacgaaag tgtcaggcac tattcggtcg
    14161 gatcagctga ttcacacctt gctgggagaa agacatttcg gagcgtaaga ggaagatatg
    14221 gcgtggtctg cgaacaagcg ctttgtccgt acggtagcag gaacctgaga cagacgtgaa
    14281 aggagatttc tcgatggcga tccggaaaaa cggcgcgcga agatgtgtga tgattgtgga
    14341 acaggatgca cactttggga tcaagctcgc agactggctc gcggctcacg gctaccagcc
    14401 ggtgctcgtt cggtccgttg atgcggcaat cgaggaactc cgagagatca gcccgcaggt
    14461 ggtctttgtc ggacgcagcc atcctgagcc cgcggcgcag aacggcatat ccgaactact
    14521 cctcctgatc cagaccctgt gccccggcgt gccggtcatt accatcgcgg atcagaccag
    14581 cgaggacctg acacacgatg ggtttcgcca agggattcac catgttctcg tcaaaccgat
    14641 cgagttcaca cagatcagtc atgtgctccg gcgggaactc agctcagtga cggtataaag
    14701 tggatt
//
LOCUS       DATIPF010000393         2882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1575055, whole genome shotgun sequence.
ACCESSION   DATIPF010000393 DATIPF010000000
VERSION     DATIPF010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575055"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="VLL06_09880"
     CDS             complement(<1..1132)
                     /locus_tag="VLL06_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSS31315.1"
                     /translation="MTEEWGAILVVDDDTEMRELVHDVLKGRGHQVTMAGSGQEALKQ
                     LTERDHAVVLTDLRMKGMSGTELLVEIRHQYPDIGVILMTAFGSVDTAVEAMKRGASD
                     YLTKPVNNEELVRVVERSIREAALRREVNRLRREVRKEYSFHQIIGKSKPIQEIFDLI
                     RRVADSPTNLLITGESGTGKELVAKAIHYNSDRQDAPFIPVNCAAIPEQLLESELFGH
                     MRGAFTDAKFDKRGLFEEAQKGTLFLDEISELPLMLQAKILRAIQEKEIRRVGANKPI
                     AVDVRIIAATNLNLTEEVKAKRFREDLYYRLNVIELRLPPLRERREDIPLLVNGFMKK
                     CAESRGKQIQGVSESALALLVDYAWPGNVRELENVIERAVTLG"
     gene            complement(1700..2365)
                     /locus_tag="VLL06_09885"
     CDS             complement(1700..2365)
                     /locus_tag="VLL06_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31316.1"
                     /translation="MRCLISVARVGGVAACAVVGLLTVIGLQPVLAIDVKLSAEEAQK
                     ALEAGRVPMEKANTPEEVKKVLQHASMVTRVGADPEKDPCGASAILRTKRYRLEAFGR
                     QEAAESKKRKMDVRMPDEFIKKVVDMPNMEIEVQLCGDDEYFAEGALIELQQGSKKIK
                     PIDIGKAERGRKNEGSGPTYRSRFTALFAYETFDPTAASVFVVNLQDGQEARIAADLS
                     KVK"
     gene            complement(2443..>2882)
                     /locus_tag="VLL06_09890"
     CDS             complement(2443..>2882)
                     /locus_tag="VLL06_09890"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HSS31317.1"
                     /translation="GSDTVIELDQGVLGKPADLVEARAMLRRLAGRDHYVHTAVALAC
                     FARAIDVVGVSTTVVRMKRFDGGVYERYLETGESLGKAGAYSIQGAGGDLVDSIAGDF
                     PTVVGLPLRLVSQLMTQAGVRVPIDLDQLYVTKPYANWARFSG"
BASE COUNT          609 a          900 c          714 g          659 t
ORIGIN      
        1 ggccgagtgt gaccgcccgc tcgatcacat tttccaattc ccgtacattg cccggccacg
       61 cgtaatccac caataatgcc agcgcggatt cgctcacacc ctgtatctgt ttaccgcgag
      121 actccgcgca cttcttcatg aacccgttca cgagcagcgg aatatcttcc cggcgttcac
      181 gcaggggagg aaggcgcagt tcgatgacat tgagccggta atacaagtct tcccggaaac
      241 gcttcgcctt gacctcttcc gtcagattaa gattcgtcgc cgcaatgatg cgtacatcga
      301 cggcaatcgg tttgttggcc cccacgcgac gaatttcttt ctcctggatc gcgcgcaaaa
      361 tcttggcctg cagcatcaac ggcagttcac taatctcgtc gaggaacagc gtgcccttct
      421 gcgcctcttc aaagaggcct cgcttatcga acttcgcatc cgtaaaggcc cctcgcatat
      481 ggccgaacaa ttcgctttcg agcagctgtt cgggaatggc ggcacagttg acggggataa
      541 acggcgcatc ctggcgatcg ctgttgtaat ggatcgcctt ggccaccaac tccttaccgg
      601 ttccgctctc gccggtgatc aagagattcg tcgggctgtc ggccacgcgc cggatcagat
      661 caaatatctc ctgaatcggt ttgctcttgc cgatgatctg atgaaaactg tattccttgc
      721 ggacctcgcg ccgaagcctg ttcacctcac gtcgtaatgc ggcttctcgg atggaccgtt
      781 ctacaacgcg aaccaactcc tcgttgttca ccggtttggt caagtagtcg ctggcgcccc
      841 gcttcatcgc ttcgaccgcg gtgtctacgg atccaaacgc tgtcatgaga atgactccga
      901 tgtctggata ctgatgcctg atctccacca acaattccgt ccccgacatc cctttcatcc
      961 gtaaatccgt caagacgacg gcatggtctc gctcggtcaa ctgcttcaag gcctcttgtc
     1021 cgctccctgc catcgtgacc tgatgcccgc ggcccttgag cacatcgtgg acaagctccc
     1081 gcatttccgt atcgtcatca acgacgagaa tggcacccca ctcctcggtc atgctgcact
     1141 ccttctctaa caggatactg aaagagtcat ctttctcacc cacccaaccc cggcgcgccg
     1201 agacgcgccc tttcccttgc atcgttctcg gcgcatcgaa atcctcaacg tacccccctt
     1261 gggaaaaaag ctgttccgac agctcgatgt tgggcgggtg gaaatgttac gcctccggtt
     1321 tcgattcgcc tgcggccttg ctggaaaact ttttgagcag cctgcgtggt cgtatcttgt
     1381 tgtcccttat gtgcgatcca ttcgatctac tatgtgccaa cataattgtt tcccctgcct
     1441 actgatggcc gactgtcaga ggctagccct ttgctctaga aatggtcaag gctgctctgg
     1501 cgcaaaagac cacacgcacc ttcccctttg gggaggaatg ccacaaaaat cgctctgtca
     1561 ttgagacttc aacaactaac aggcagaggg tgcaaaggcg gggccaagta tccggtggag
     1621 gaaattcatg ggcggctgtt tgtggagaaa caggagcagc tgccgaagat tggcacggga
     1681 caggatcgaa gacgtgtgcc tacttgacct tggaaagatc ggcagcgatt ctcgcttctt
     1741 gtccgtcctg aagattcacg acgaaaacag acgccgcggt gggatcaaat gtttcatagg
     1801 caaacagcgc ggtaaagcgc gatcggtagg tcggcccgct tccctcattt tttcggccac
     1861 gttcggcttt tccaatatcg atgggcttga tcttcttaga gccctgttgc aactcgatca
     1921 aggccccttc tgcaaaatat tcgtcatcgc cacacagctg aacttcgatt tccatattgg
     1981 gcatatccac gactttcttg atgaactcgt caggcatacg cacatccatc ttccgcttct
     2041 tcgactctgc cgcttcctgc cggccgaagg cttcaagccg atagcgtttg gtccggagga
     2101 tggcactggc tccgcaggga tctttttccg gatcggctcc cacacgcgtg accatggagg
     2161 cgtgctggag gaccttcttc acctcctccg gggtgttggc cttctccatc ggcacccgtc
     2221 cagcctccaa ggccttctgt gcctcttcgg ctgagagctt cacgtcaatg gccaggactg
     2281 gctggagacc gataaccgtc aacaggccca caaccgcgca ggcggcgaca ccaccaactc
     2341 gcgccactga tatcaggcat cgcacgcgca tccctccttc ccgaaacacc acgaagaatg
     2401 ttggggcggc attgtacgaa aacctctacc cctttgcaat aatcagcctg agaagcgagc
     2461 ccagttggca tagggcttgg tgacatagag ctggtcgaga tcgatcggca ctctcacacc
     2521 cgcttgcgtc atcagctgag aaaccagacg aagtggcaaa ccgaccacgg ttggaaaatc
     2581 tcccgcaatg gaatcgacca agtcgcctcc ggctccttga atcgagtacg caccggcctt
     2641 gccgagactt tctcccgttt ccagataacg ttcgtacact cctccatcga atcgcttcat
     2701 ccgaacgaca gtcgtagata caccgacgac gtctattgca cgagcgaagc aggcgagcgc
     2761 gaccgctgtg tgcacataat gatcacgacc ggccagacgt cgaagcatcg cacgggcctc
     2821 taccagatcg gccggcttcc cgaggacgcc ttgatccaac tcaattaccg tatcgctccc
     2881 ga
//
LOCUS       DATIPF010000394         4837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_343413, whole genome shotgun sequence.
ACCESSION   DATIPF010000394 DATIPF010000000
VERSION     DATIPF010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343413"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VLL06_09895"
     CDS             <1..455
                     /locus_tag="VLL06_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSS31318.1"
                     /translation="HFLGLVSEQSLYNLNERTIELEVIPACESYGIGLIPWSPLARGL
                     LAGALDPAKVGRRADEDVKKDIEKYRPRLEAYAALCTQLGEQQADVALAWLLHQKAVT
                     SPIIGPRTMEQLNGTMRVLNLTLTKDTLKQLDHIFPGPGKPAPEAYAW"
     gene            475..1128
                     /gene="thiE"
                     /locus_tag="VLL06_09900"
     CDS             475..1128
                     /gene="thiE"
                     /locus_tag="VLL06_09900"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HSS31319.1"
                     /translation="MRDKRGGIDGIYVILDPSVCPDRSLTDVLKEAAAAGVRLFQYRN
                     KSASMKEAYGEALPLRTIARDTGVTFIVNDRCDLALAVDADGVHLGQEDLPLDLARKV
                     MGPDKLIGISTHNPDQVREATAGKPDYLGFGPIFKPGSKHDHDPVVGLEGLRAMRTLT
                     SLPVFAIGGIQIDQAGEVMRAGANGVAIISAILKARDISHAVKSFLAQMPGPTSLTS"
     gene            complement(1067..1849)
                     /gene="bioD"
                     /locus_tag="VLL06_09905"
     CDS             complement(1067..1849)
                     /gene="bioD"
                     /locus_tag="VLL06_09905"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HSS31320.1"
                     /translation="MRRSKRHAHDSSSRGVFITATDTGVGKTVVTAALAVALRTHGYA
                     VGVMKPIETGVRSSSDAESDAARLRTAAGSSDTLTEIRPYAFRLPLAPMDAARFEKHT
                     ISLPTIVQALRTLRSRHEVLLAEGVGGVHVPLTSVFDGLDLIYRMRFPVIVVGRVGLG
                     GINHALLTLEALRRRHVSVLALVLNQTVPARTVTARVQEGSTVHLLRQRAGVPMIGPL
                     PYCSTLKSNFHMEIAKLAKTAAITKLMRLVLASGQGTISRRG"
     gene            complement(2014..2754)
                     /locus_tag="VLL06_09910"
     CDS             complement(2014..2754)
                     /locus_tag="VLL06_09910"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSS31321.1"
                     /translation="MTRPATESQQAVLVTGASGGIGRAISLAFAAAGWSVGVHYHRNK
                     AAAEDTLRQVVEVGGAGALYEADTREPYAVQQMVEASCRLVPIPSVFICNAGIGGSSL
                     VLTQREEDWAEVLATNLTGTFHCLRAMAPPLLASGGGSIVVIGSHAGYHGSVGQAAYA
                     ASKAGLIGLVKTAAQEWGSQNIRVNLLLPGWQRTELSKGMMPESAGWTDHALRRSPSI
                     EEIAKTVLHLAQLTDVGGQVWNCDSRNL"
     gene            complement(3718..>4837)
                     /locus_tag="VLL06_09915"
     CDS             complement(3718..>4837)
                     /locus_tag="VLL06_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSS31322.1"
                     /translation="LASPKRLGHEPGELNERLYSPRGVTAVIAPWNFPLAIPTGMVSA
                     ALVTGNPVVFKPSERSPMMGYWLTEILREAGVPEGVVCCLPGGPEIGRLLVHHPDIVT
                     IAFTGSKDVGLGILKDAGSLMPGQHMVKRVLAEMGGKNAIIVDETADLDDAITGVVSS
                     FTGYAGQKCSACSRAIVHAAVYESFLDRLKEAVLSLKVGNPEEPGTQVGPVIDRRAQS
                     KIQEYIEIGKKEARLLVEGTVTGPGWLIGPTVFVDVASTHRVAQEEIFGPILAVMKAA
                     SFEEALTLANATAYALTGGVYSRSPANLELARERFDVGNLYLNRPITGALVSRQPFGG
                     HRLSGVGAKAGGEEYLTQFVVTRVVSEQTLRRGFAPPE"
BASE COUNT         1030 a         1453 c         1377 g          977 t
ORIGIN      
        1 gccatttcct cggcctcgtg tcggaacaga gtctctataa cttgaacgag cggacgatcg
       61 agttagaagt gatccctgcc tgcgaatcct atggcatcgg gctgatcccc tggagtccgc
      121 tcgcgcgcgg gctgttggct ggggcgttag atccggcgaa ggtcggtcga cgcgcagacg
      181 aggatgtgaa aaaggacatc gagaaatatc gtcccagact ggaagcctat gccgccctct
      241 gcacacaact cggcgagcag caggccgatg tggcactcgc ctggctgctg catcaaaagg
      301 ccgtcacgtc gccgattatc gggccacgta cgatggagca attgaacggc acgatgcgcg
      361 tcctgaacct cacgttgacc aaggacactc tgaaacagct cgatcatatc ttccccggtc
      421 caggcaaacc tgcaccagaa gcctacgcct ggtaaaccgc aagaggtgtg agacatgcgt
      481 gacaagcgag gtgggatcga tgggatctac gtcatacttg acccatcggt ctgtcctgat
      541 cgttccctca ccgatgtgtt gaaggaagcg gcggcggccg gggtacggct gtttcagtat
      601 cggaataagt ctgcctcgat gaaggaggcc tacggtgaag cgttgccgct gcgaacaatt
      661 gcgcgggaca cgggcgtgac attcatcgtg aacgatcgct gcgacttggc cctggctgtg
      721 gatgccgacg gtgtgcatct ggggcaagag gacttgccgc tcgacttggc caggaaggtg
      781 atgggccctg acaagctgat cggaatctcc acgcacaatc ccgatcaggt gcgggaagcc
      841 acggccggga aaccggacta tcttggcttc ggtccgatct tcaagccagg gtccaaacac
      901 gaccacgacc cggtggtggg gcttgagggg ttacgtgcaa tgcgcacgct cacatcgttg
      961 ccggttttcg ccattggcgg gattcagatc gatcaggcag gggaagtgat gcgtgccggc
     1021 gcgaatggcg tggcaatcat ttcagcgatc ctcaaggccc gggatatcag ccacgccgtg
     1081 aaatcgttcc ttgcccagat gccaggacca acctcattaa cttcgtgatc gcagctgttt
     1141 tcgccagttt cgcaatttcc atgtgaaagt tgcttttgag ggtagaacaa tagggcagtg
     1201 ggccgatcat cggcacaccc gcgcgctgcc gaaggagatg cacggtcgag ccttcttgca
     1261 ctcgtgctgt cacagtccgt gctgggacag tttgattgag aaccaatgca agcacggaga
     1321 cgtgtcgccg gcgaagtgct tcaagggtga gcaacgcatg attaattccg ccgagtccaa
     1381 cccgtccgac gacaatcaca ggaaacctca ttcgatagat gagatctagt ccgtcaaata
     1441 ctgaagtgag cggcacatgc accccgccca caccttcagc gagtaacacc tcgtgccgcg
     1501 atcgaagtgt gcggagggct tgcacaatgg tgggcagcga gatcgtgtgt ttctcaaaac
     1561 gagccgcatc catcggagcc agtggcaagc gaaaggcata gggcctgatt tcggtaaggg
     1621 tgtcgctgct tcctgctgct gtgcgaagtc gtgcagcatc ggactcggcg tccgaggatg
     1681 acctcacgcc cgtctcaatt ggcttcatca ccccaactgc atacccgtga gtgcgcaagg
     1741 cgacagccag cgcagccgtc accaccgtct tcccaacacc cgtatcagtc gcagtgataa
     1801 atacccctcg tgagcttgaa tcgtgagcat gtcgtttact tcttctcata attaaaatct
     1861 gatggaaacc tgaagtctcg ctccctgcag gacgctcaaa acgtctgtcc agcaaggccc
     1921 gagctgaaga aagcaccgga ggcgtaccct ctcgggtacg tcgaggatgc tttcgaggcg
     1981 agaacgcagc gggcggacgt tttcagcgtc ctgctataga tttcggctgt cgcagttcca
     2041 cacctgcccc cccacatcgg tgagctgcgc cagatgcaac acagtcttgg cgatctcctc
     2101 aatcgaagga gatcgacgca gggcatgatc cgtccatccc gccgattcgg gcatcatacc
     2161 cttcgacaat tccgtccgct gccaaccagg cagtagaaga ttcacgcgga tgttctgcga
     2221 tccccattcc tgcgccgcgg tcttaaccaa tccaatgagg ccggcttttg atgccgcata
     2281 ggctgcctgc ccaacggagc catggtaccc ggcatgcgat ccgatcacga cgatcgatcc
     2341 gccgccgctg gctaggaggg gaggggccat ggcgcggaga cagtgaaatg ttccggtcag
     2401 attcgtggcc agcacctcag cccaatcttc ttcccgttgt gtgagcacaa ggctgctccc
     2461 tccaattccc gcattgcaga taaacactga ggggataggt acgaggcggc aggaggcttc
     2521 taccatctgc tgcaccgcat agggctcgcg ggtatcggct tcgtagagcg ccccggcccc
     2581 accgacttcc actacctgcc tgagtgtgtc ttcagccgcc gccttgtttc gatggtaatg
     2641 gacccctacg gaccagccgg ccgctgcgaa ggccaggctg atggcgcgac caatccctcc
     2701 ggatgcaccg gtcacaagca cggcctgttg agattcagta gcgggtctcg tcatgtcact
     2761 cccttgtccc ttcgtagcca ctgggcagcc cctgttgaga catcaggata tcgtctaaca
     2821 ccacggctcg aacgaagcaa gactgcagca gtgatacccc acttctcttg ctgcctccag
     2881 gaggcctatg gtacggtcac ccgtacgttc aaccagggga gaatcatgcc catgctgctc
     2941 ataacgccgg gatatcggat ggccctcatg ctgggaacag cttccctgct cgtcttaagc
     3001 acctggtcag cggctcttgc agcggaagag gccgccatca tcacccagtc cgtggactat
     3061 ttccccgatc agattgggaa cgaatggcgg taccgcggcc agattactga agggccgctc
     3121 caaacgatcg aacataaatt tttctctaat gtgtcctcgg tcgccggcac gaaaaccctc
     3181 aaggggatga cggtcaccgt ctatcacgat agcaatccgg gcaaccatgg cccttcagat
     3241 agtttctacc gccgcgatag tgtcggcatc gtctactacg gctcagaacc tggcacaccc
     3301 ttcgaaaaag aactcgtgcc ctatcagatc gtacgctttc cgatgaaagt ctcgtcttcc
     3361 tttgcgcagt tcaaccgcaa gggggtgaat tttggtgccg atctggatca ggacggcgtc
     3421 gatgaaaagg ccgatgcgca gggcgattcc acgattgtgg gacgggaatc ggtcaccgtg
     3481 ccggccggca cattcaagga cgcggtgaag gtggaagcgc gcatgttcat gcagattcat
     3541 ctctccggca cgaagaagac cgcggtcggc accgatgtga tgaccgcctg gtttgtgaag
     3601 ggcgtgggct tggtcaaata cgtcgagcgg caggaactat cgccgttgga agaccggggt
     3661 gtggttaccg atatcatgga agagctggaa tcgtacgaga tcaagccgcc gaaggcctca
     3721 ctcggggggc gcgaatcctc gccggagagt ctgttcgctg accacgcggg tcacgacgaa
     3781 ttgggtcagg tactcttcac ctccggcttt cgctccgact ccggaaaggc gatggcctcc
     3841 aaacggctgg cggctgacta gcgcgccggt gatgggacgg ttcagataga gattcccgac
     3901 gtcgaatcgc tcccgtgcca gttccagatt ggccgggcta cgcgaataga ctccacccgt
     3961 cagtgcatag gccgtggcat tggccagagt cagcgcctcc tcaaaagaag ccgccttcat
     4021 caccgcgagg atcggcccaa agatttcctc ttgagccact cgatgtgttg aggccacatc
     4081 cacgaagacc gtcggtccga ttaaccaccc gggtcccgtg acagtccctt cgaccaacag
     4141 gcgggcttcc ttcttcccaa tctcgatgta ttcctgaatc ttcgactgcg ctcgccgatc
     4201 gatcaccggc ccaacctgag tgccaggctc ttctggattt ccgaccttga ggctcagcac
     4261 tgcctctttc aggcgatcca gaaaactctc gtacaccgca gcatgcacaa tggcccgtga
     4321 gcaggcggag cacttctgcc ccgcatagcc ggtgaaggat gagaccactc cggtgatcgc
     4381 atcatcgaga tccgcagtct catccacgat aatcgcgttt tttcctccca tctcggccag
     4441 cacccgcttg accatatgtt gccccggcat cagactacca gcgtctttga ggatgccgag
     4501 ccctacgtcc tttgagccgg taaaggcaat cgtcacgata tcggggtggt gcaccaacag
     4561 acgtccaatc tcaggtccgc ccggtagaca gcacacgact ccttcaggca caccggcctc
     4621 tcgcaagatc tctgtgagcc aataccccat catcggcgac cgttcggagg gtttgaagac
     4681 cacaggattc ccggtcacga gtgccgccga caccatgccg gtgggaatgg ccagcgggaa
     4741 attccagggc gcaatgacgg cggtcacccc acggggacta tagagacgct cgttcagttc
     4801 acctggctca tgccctagtc gttttgggga cgccagc
//
LOCUS       DATIPF010000395         4130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_695206, whole genome shotgun sequence.
ACCESSION   DATIPF010000395 DATIPF010000000
VERSION     DATIPF010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695206"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VLL06_09920"
     CDS             <1..526
                     /locus_tag="VLL06_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSS31323.1"
                     /translation="DDITIRAILNHSDVSALGHYCFKSFDSLADPIQRYADWLWGLHD
                     STAINRKPVVPPPAATVQQAQRHAPVIAPPLYTQPPMRMPVRQSGMRPLSGRERQIIA
                     LIADGRSHKEMSDTLGLSIPTIVCYRGRLLDRLQLTTTAELIVYAREHALTKVPMVVI
                     KRGRIGFDRTQATR"
     gene            complement(1472..1648)
                     /locus_tag="VLL06_09925"
     CDS             complement(1472..1648)
                     /locus_tag="VLL06_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31324.1"
                     /translation="MKHFNVHDDHKKDILHDAFMLLIPPLLILICTLWLFDVIRDVAP
                     FLVSLIDSSLARLR"
     gene            1747..2217
                     /locus_tag="VLL06_09930"
     CDS             1747..2217
                     /locus_tag="VLL06_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31325.1"
                     /translation="MARSTKGLYKRGNVWWIMYFDALGHQRFESSKSSNKRDAEKRLV
                     DRRKEAHEELLPAPPIKPLSLDDLRQRYLAFVGHQRGVATKHIHFDHFARVWSNPPIH
                     TLTVEVLDQYRARRIGEKVGPATINREISTLKHALRSAAVPVRTGGCRSAPRSL"
     gene            2150..2650
                     /locus_tag="VLL06_09935"
     CDS             2150..2650
                     /locus_tag="VLL06_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HSS31326.1"
                     /translation="MPSGRLRYLSGPAGADRLLAACEDSLKPIVLTALHTGMRKEELL
                     SLTWDAVDMTHGFIRLKQTKNGTVRALPFNETLWSLFNGLRTRQDVLWVFHDGEGRWY
                     LDVRHAYERACKGAGLTDFHFHDLRHTFASWLIMRGVALATVSNLLGHTSPTMTLRHV
                     HLSPNI"
     gene            2799..3245
                     /locus_tag="VLL06_09940"
     CDS             2799..3245
                     /locus_tag="VLL06_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31327.1"
                     /translation="MRDARSRFPDTVKLEVPLDVSPRLFIERRIKSRAIVDGGLASFS
                     SLEDCDAAEGDTALLNLSCGGCQLHVEEHLPQGQPYQLIVFVPLYAVPILIRKAVTQW
                     AQGQIHGVKFLELDELASLKLAEAVRCSPVSSWVLNSIRFSMWSPL"
     gene            complement(3414..3500)
                     /locus_tag="VLL06_09945"
     tRNA            complement(3414..3500)
                     /locus_tag="VLL06_09945"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3464..3466),aa:Leu,seq:cag)
     gene            complement(3562..>4130)
                     /locus_tag="VLL06_09950"
     CDS             complement(3562..>4130)
                     /locus_tag="VLL06_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31328.1"
                     /translation="QVSDLQAKIKEVNTTLAEYENLPLLRRLSLQAVGKNVESFQQYI
                     ELYESQCAELREELDTAKLRIDELVPEAAVPKDMRPEFDELKEEVIRLGGTKKIRELL
                     AAEEDTNRQAFIQNRRLVVTTASRVVSDPLFSRVRFDVFIADEAPWIEAAPLLGAAGL
                     VRERIVISGDQRDIAAAGRWTSHESQLR"
BASE COUNT          899 a         1179 c         1189 g          863 t
ORIGIN      
        1 ggacgacatc accattcggg cgatcctaaa tcattccgat gtgtcagccc tcggacacta
       61 ttgctttaaa tcctttgact ccctggccga ccctattcaa cgctatgccg actggttgtg
      121 gggcctgcac gactcgaccg cgatcaaccg caagcccgtt gtgcctccac ctgcggcgac
      181 ggtgcagcag gcacaacgtc acgctccggt catcgctcca ccgttataca cccagccgcc
      241 tatgcgcatg cccgttcgac agagtggcat gcggccttta tcgggacgag agcggcaaat
      301 catcgcgctc attgcggatg gccgatccca taaggagatg tccgacaccc tggggctgag
      361 tattcccacc atcgtctgct atcgcgggcg cttgctggac cggctccaat tgaccacgac
      421 cgctgaattg attgtctacg cgcgagagca tgcgctcacg aaggtgccga tggtcgtcat
      481 caagcgaggc cggattgggt tcgatcgcac acaggctacg cggtagagcg cgaggaatgg
      541 ccggggtgag tcaggttgac ggtttcctga tccatttatt catgcacgat ggagtctggc
      601 aggcccacag gggcatggga agcccctgtg gattgtggtg ggacacgagg cagctccacg
      661 ccggtctgac atcataggca gaacatttcg ggcattggac gggcacatta gtcctagcaa
      721 caccagctag tctgggcatt ccggcctcca tgcggattcg aggtccaaag ggattctcga
      781 gtgttctcat gctacggcgt tccgcctcct tgttcatgtg taaaagtagc gtacggtcat
      841 tccattaaga cgatgcgggg cctcacaaca tgacgctggc aatatcgagc aggagacaag
      901 gatcgaggct tgttgagttg gaaggatacc accggctagg ggagcaggaa tgcgtggaag
      961 ggggaaaatg gatttctcaa gcgatacgcg actcggcctc tttcaaagcc gagccgtata
     1021 gatcaggtcg tctgaataaa ccatgcagtc cattaaggcg acaaccatgc aatcgcgata
     1081 gagataccga tgattggcac cacaatgacc ggcgtcaaga tgatgagcat gtctagtagg
     1141 aacgtcccat caaaaaacgg cttcatgggg acctccttcc atgacacgat gaagaaatca
     1201 gtactcggca ggatggtgga acgatggctc agccgtagaa caagagaaga atcatcgcac
     1261 ccgtcgcggc gatcggtacc aacaaagtca gcgagtctcg catcacggtg tcacgatact
     1321 gttgacgacg acgccggggg ctcatgatgg atctaatatc caacccgggg ctaaatgtcg
     1381 gcactgggaa gttcccgagg ttacccaagt cgattggatt cactctgggt aggaggtggt
     1441 agcggtttac gacgcagaaa ctcccattcc ctcacctcag gcgagcgagg gatgaatcta
     1501 tcaaagagac aagaaacggg gcgacatcac ggatcacatc aaagagccag agcgtacaga
     1561 tcaggatgag caggggcggt atgaggagca tgaaggcgtc gtgcagaata tccttcttgt
     1621 gatcgtcatg aacattaaag tgcttcatgc tcattatttc acgccaccac gtcacgaatt
     1681 tccattgggt gaatccccta aatttgaggc tggagatgca cggaacaacc tgaggagggc
     1741 tgagcgatgg cacgatcgac gaagggactc tataagcgtg gcaatgtgtg gtggatcatg
     1801 tacttcgatg ccctgggcca tcagcggttt gaatcgtcca agtcctcgaa caagcgagac
     1861 gcggaaaagc gtttagtgga tcgacggaaa gaagcccatg aggagctgct gcccgcgcca
     1921 cccattaagc cgctctcctt ggacgacctc cgacaacgct atctcgcctt tgtcggacac
     1981 caacgaggcg tagccaccaa gcatattcat tttgaccact tcgcgcgcgt atggagcaat
     2041 cccccgattc atacgctcac ggtggaagtc ctggaccagt atcgggctcg gcgaatcggg
     2101 gagaaggtcg ggccggccac gattaaccga gaaatctcga cgttgaagca tgccctccgg
     2161 tcggctgcgg tacctgtccg gaccggcggg tgccgatcgg ctcctcgcag cctgtgagga
     2221 ctcgctcaag ccgattgtgc tcacggcact ccataccggg atgcgcaagg aagagctgct
     2281 ctccctcacc tgggatgcgg tcgacatgac ccatgggttt atccgcttaa agcagaccaa
     2341 gaacggcaca gtcagggcgc tgccgttcaa tgagacgctg tggagcctct tcaacggact
     2401 ccggacccgc caggatgtgc tctgggtgtt tcatgatggt gaaggccgat ggtatctcga
     2461 tgtacggcat gcctatgaac gggcctgcaa gggagctggc ttgaccgatt tccacttcca
     2521 tgacctgcgg cataccttcg cgtcctggct catcatgcga ggggtggcgc tcgccacggt
     2581 cagcaatctg cttgggcata cctcgcccac catgacgctt cgccatgtcc acctgtcgcc
     2641 gaacatttga cctcacggtg cgggtgctgg atgcagacca tggacccgct cagagcattc
     2701 agagggaagc acccgcaggc ccggcggggt cacgagcgga gcgccttgtg gtctctcggg
     2761 tgttgtgata catagcagag ccacaagagg caccgagaat gcgagatgct cgaagccgct
     2821 tccccgatac ggtaaagtta gaagtcccgc tcgacgtttc cccacggctg ttcattgagc
     2881 gccgaatcaa atcacgagcg atcgtggatg gtgggctcgc gtccttcagt tcactggaag
     2941 actgtgacgc ggctgaagga gataccgcgc tcctgaacct ctcgtgcggc gggtgtcaat
     3001 tgcatgttga ggagcacctt ccccagggtc agccgtacca actgattgtg tttgtcccgc
     3061 tctacgccgt cccgattctc atccggaagg cagtcacgca atgggcacag ggccagatcc
     3121 atggggtcaa gtttctcgag ctcgacgagc tggcgtccct caaactggcc gaggcggtcc
     3181 gatgtagtcc cgtctcctcc tgggtcctca acagcatccg gtttagcatg tggtccccct
     3241 tataatccct tttcagtctc ggttctcgca cggacatcat ggggttcagc gccctgttcg
     3301 cactcgtcac acgacaattg acccgaaaca ggcctccaca accgtgtgag ccgatgggat
     3361 aaatgaaggg gttgaaacgt cgaaacgcct tgctagaaaa ggggaatatg agttggtgcc
     3421 gaaggcggga tttgaacccg cacacccttg cgggcgctag accctgaatc tagtgcgtct
     3481 gccagtttcg ccacttcggc acatcgaggt ggaggcggat tatcctgaat tatcgtggcc
     3541 ccgtcaagca agcggcttca atcaccgaag ctgcgactcg tgtgaggtcc accgtccagc
     3601 tgccgcaatg tcgcgttgat cgccgctgat cacgatccgc tcacggacca gcccggcggc
     3661 gccgagcaat ggggctgcct cgatccatgg cgcctcatcg gcgatgaaga catcgaaccg
     3721 gacgcgactg aacaggggat cactcacgac tcgcgaggcc gtcgtcacca cgagccgtcg
     3781 attctgaatg aaggcctggc gattcgtgtc ttcttccgcc gcgagtaact ccctgatctt
     3841 tttcgtcccg cccagccgga tgacctcttc tttgagctcg tcaaattccg gtcgcatatc
     3901 tttcggtacc gcggcttccg gcacaagttc atcgatgcgc aacttggcgg tatcgagttc
     3961 ctctctcagc tcggcgcact gactctcata cagctcgata tattgctgga acgattcaac
     4021 gttcttgccg acggcctgca ggctcagccg tcgcaacagt gggaggttct catactcagc
     4081 cagcgtggtg ttcacctctt tgatcttggc ttggaggtcg ctgacctggg
//
LOCUS       DATIPF010000396         1577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1575382, whole genome shotgun sequence.
ACCESSION   DATIPF010000396 DATIPF010000000
VERSION     DATIPF010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575382"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="VLL06_09955"
     CDS             complement(<1..1208)
                     /locus_tag="VLL06_09955"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoD-like phosphatase N-terminal
                     domain-containing protein"
                     /protein_id="HSS31329.1"
                     /translation="MIPSRIAFRKSLFHSVLPGHAHGKAAWKGKLLIGLCGLFLAVSV
                     VSCAKPLFPPAAVEDLDPALQMGISNPEADVYFKDHLAQAGGRIIAIEQTANGSLITA
                     EELPLTHAGTEVVETAKPTGWFVFLYKGRIDPLGLQQGNKFIVVGLVKGTQTVTINGV
                     QRLAPYVVARCVHVWKTGRYAIADFPNVPGGYYPLEHQTYCLDSPFEMSASSGTGAVS
                     HGIATGDVTSDGAVIWFRTDGPARVEVRVATVKNWEQFVPGEAPQQSLMKTELVSANK
                     EQDFTVKVPLTGLIPATRYRFDIRTIQPAQSPRSTTITTQGEFMTAPSSNEFAPTTFL
                     WSADLGGQHRCRDEQTGYPIFDVLRDQKADFTILLGDLIYGDDTCPSPPNATGSDFTA
                     STLTQYRAKHR"
BASE COUNT          370 a          416 c          434 g          357 t
ORIGIN      
        1 cgatgcttcg ctcggtactg agtgagggtg gaagccgtga agtcgctgcc tgtggcattg
       61 ggaggagatg ggcacgtgtc atcaccgtag atgagatcgc ccagcagaat cgtaaagtcc
      121 gccttctgat cccgcagtac atcgaaaatc ggatagccag tctgttcatc ccgacaacga
      181 tgttgtcccc caagatcggc actccacaga aacgtggtgg gagcaaactc gttcgacgaa
      241 ggcgcggtca tgaattcgcc ttgtgttgtg attgtcgtag acctaggcga ctgtgcgggc
      301 tgaatggtgc gaatatcaaa gcgatagcgg gtggctggga tgagccctgt cagtgggacc
      361 ttcaccgtaa agtcctgctc cttattcgcg gagaccagtt cggttttcat caaagattgt
      421 tgtggcgcct ctcccggtac aaattgctcc caattcttga ctgtggccac acggacttcg
      481 acccgagcgg gaccatccgt ccgaaaccag atgacggcac cgtcgctggt cacatcaccg
      541 gtcgcaatgc catgggacac agctccagtc cccgaggaag cggacatctc gaacggactg
      601 tcgagacaat atgtttggtg ttctaatggg taatagccac caggcacatt cgggaaatcg
      661 gcgattgcat aacggccggt cttccacacg tgcacacacc gggcaaccac ataaggggcc
      721 agccgctgga cgccattgat ggtgactgtt tgggtgcctt tcacgagtcc caccacgatg
      781 aatttatttc cttgttggag ccctaaagga tctatgcgtc ccttgtagag aaacacaaac
      841 cagccagtcg gtttggcagt ctccacaacc tctgttccag cgtgagtgag aggcaactcc
      901 tcagctgtaa tgagagatcc attagccgtc tgctcaatgg caatgatccg accgcctgct
      961 tgggccagat ggtctttaaa atagacgtcg gcctctggat tagagattcc catttgcaga
     1021 gcagggtcca gatcctcgac cgcagcgggt gggaacaagg gtttcgcaca tgacaccacc
     1081 gacacggcaa ggaacagccc gcataagccg atcagaagtt tgcccttcca cgcagccttc
     1141 ccatgcgcat gaccgggcaa cactgaatgg aatagggatt ttctgaaggc gatacgtgaa
     1201 ggaatcatgt atgtaggatt ctctctttcc gatgtgcgtg catcgctggg ttcaagcatt
     1261 tcatttgcgc ctcactcgtt atgttcaacg ggttactact gtgaccatct ccaggtagaa
     1321 ttatcgcatg ctcctgacca aattgtatga cgaaggggcg tcaaatcaga aggtgagagg
     1381 aggtacggac acgctcctaa acgcgatgat aggtggactg gtttaagtgt aggagcagcg
     1441 gtttaagaaa gtgttgtcac tctcctactc cattaagcgg gagacgatac tctgccggga
     1501 cggacgcttc gtcacttgcc atcagcccgc tgcttcgtga tcgagacctt cccccagaag
     1561 acatagtggg catagac
//
LOCUS       DATIPF010000397         5424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1228439, whole genome shotgun sequence.
ACCESSION   DATIPF010000397 DATIPF010000000
VERSION     DATIPF010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228439"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..892
                     /locus_tag="VLL06_09960"
     CDS             122..892
                     /locus_tag="VLL06_09960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSS31330.1"
                     /translation="MVPLLCQEYLPRMTVPSHPWASVIVPIKDERDNLVPLTEQLLKA
                     LESCDESRSAPFEILFVDDGSTDGSSDLLDRLAADYPRVRVLHFDRNYGQSSAFDAGF
                     KYSTGDLVITIDGDLQNDPADIGTLLPHVKTFDLVCGWRTNRHDNLVRTVSSRIANAV
                     RGAVTSDRVHDTGCSLKIFRRAVVDKLQLFNGMHRFFPALALMHGFTVTEVPVRHYPR
                     AHGLSKYGVGNRLFNSLYDLIAVRWMQTRALRYRFRNK"
     gene            1020..1328
                     /locus_tag="VLL06_09965"
     CDS             1020..1328
                     /locus_tag="VLL06_09965"
                     /inference="COORDINATES: protein motif:HMM:NF019214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HSS31331.1"
                     /translation="MMTITIEHIWLAIGFLGQGLFFGRWVIQWIASEKKAESQVPVAF
                     WYMSLIGGLITLAYAIYRKDPVFIAGQSIGSLVYIRNLMLIARASQASQPASPSPPRT
                     "
     gene            1386..3122
                     /locus_tag="VLL06_09970"
     CDS             1386..3122
                     /locus_tag="VLL06_09970"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSS31332.1"
                     /translation="MITTDRSHHAVLLLALLLLSGLLFFLGLGEMGLTDQDEGRNAEA
                     GREMFESGDHLTPRFNGELRVAKPVLIYWLMEQSYRLFGVNEFAARFPSALFGVGLIL
                     IHYLFLVHQRDRTVALFGGLMLLLNLEILGLGRMALTDSVLIFFTTASLYGFWLGLHG
                     EGVVRRWMWMFYIGMALATLTKGPVGFAVPLIAAALYLTWTRRWRDYWQKGMPLAGML
                     LFALLAAPWYAAMLYVHGDAYASAAKTNTIGRFLSPMEGHYGTIFFYIPVLLVGFFPW
                     SALLPLPLYHTFKEWYLIRRARARPDPTGTTELDLFAALWVVSVFVFFTASATRLPHY
                     IGPLFPAAALLTASYWSRGLDDPHTKGIRASIHLMMGVGYLLAIGFACLPTLYTTYAD
                     KMVREFPLAGQVDLGIGPYLVAALLLFGMTLVGYFGLNDERRGEAFWAAGGTLAGLVL
                     IVIVIAIPHLNRYAVAPAQELAYAAGLNLTPQDQFIAYGTTRASSVFYARRKTLFVHR
                     GEADMIRAALKEPKRVMILLPENAQSKLPAEAIGLVPILKRYGYILLANQPMVTIPEG
                     APPPPPPKILGH"
     gene            complement(3217..4311)
                     /locus_tag="VLL06_09975"
     CDS             complement(3217..4311)
                     /locus_tag="VLL06_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HSS31333.1"
                     /translation="MALPRDTIHSSRFVPVAAFLLSVCALILTFAGLFEWDVPLTRFI
                     RSLNDVHRDHLANPWLAQLSDFGDLLGSGESLVILSLVLLAVGYGFKTPQWKEAGWQS
                     LIAHGLAALSVNILKHMIGRPRPKFMHAGNLEFSPASGSGWDSFPSGHASAAFAVATV
                     LAIKFPRTKWPILAVAAVIAASRIVRGSHYLTDAAGGAALGCVMGMIAAHPWREWRAS
                     IGTAVCRITPFFVATLAFVWTIVHLPSEAWPFQQLVWGGLFLTLAGLVGHVLWLVQSL
                     SWRPAWLTGSLTRGLVGLGLGMTTGSLLVTAVVLLACAAYWLNNLPGQIEPMTEGPVG
                     LWAAMAEGLFVAAMLLVLGASYALKGIVPM"
     gene            complement(4550..>5424)
                     /locus_tag="VLL06_09980"
     CDS             complement(4550..>5424)
                     /locus_tag="VLL06_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31334.1"
                     /translation="LKPADAGLGCYQMYVMDLDSGTVRLVSTGAGATTCGYFFPGDRR
                     ILYSSTHASGPNCPPKPKRDGAYRWALDDYDIFAVGIDGQEMQRLTFTPGYDAEATIS
                     PDGKTIVWTSVKDGDLDLHAMNLDGSKPRRLTNEVGYDGGAFFSPDSTRIVYRASHPT
                     DQAEIDKYKDLLNQRLVEPGQLEIFVMNADGSGKRAVTSNGASNFSPFYFPDGKRVIF
                     SSNIETKGEGGRPSFHLYAIGEDGQGLERLTFDGQFNSFPMFSPDGTSLVWVSDRKAK
                     TPGEFNVFLADWVP"
BASE COUNT         1164 a         1615 c         1483 g         1162 t
ORIGIN      
        1 caagtcgatc tctcgcatat cttgcccatc tcgctgtttc tgctgctcgc gtccgtcgtg
       61 cagaccatgc tgtatggttt gacgctccct ccggcattct gtagaatgcc ggagggattg
      121 tatggttccc cttctgtgtc aggaatatct accccgcatg accgtgccat cccatccatg
      181 ggcttccgtc atcgtcccta tcaaggatga gcgagacaat ctcgttccac tgactgaaca
      241 attgctcaaa gccttggagt cctgtgacga atcacgctca gctcccttcg aaatcctctt
      301 cgtcgacgac ggcagcacgg acggaagttc cgacctgctc gatcgactgg ctgcggacta
      361 tccgagagtg cgcgtcttgc atttcgatcg caattatggt caatcatcgg cattcgacgc
      421 gggattcaaa tactcgaccg gcgatctggt catcacgatc gacggcgacc tccaaaatga
      481 cccggctgat atcggcacac tgctgcccca tgtgaaaacg ttcgatctcg tgtgcgggtg
      541 gcggacaaac cgacatgaca acctcgtgcg aacagtgtca tcgcgaattg ccaacgccgt
      601 gcgtggcgcg gtcaccagcg atcgggttca cgacaccggt tgctcgttga agatcttccg
      661 ccgtgccgtc gtggacaaac tgcaactgtt caatggcatg catcggtttt tccccgcgct
      721 ggcgctgatg cacggcttca cggtcaccga agtgcccgtg cgccattatc cccgcgcgca
      781 cggcctgtcg aaatatggtg tggggaaccg cctcttcaac agcctctacg acctgatcgc
      841 cgtacgctgg atgcaaactc gagcgctacg gtatagattc cgcaacaagt gatggatagg
      901 ccggaccagt gaaaaaggcg agagatggac gacacacaag attccatgga ccttgagcat
      961 cggcaacctc gcacgtcttg cgttcatcgc tcgtctcgcc actctcacct gacgactcca
     1021 tgatgaccat cacgattgaa catatctggc ttgccatcgg atttctcggg caagggctgt
     1081 tttttggccg ctgggtgatc caatggattg cctccgagaa aaaggcggag agtcaggtcc
     1141 ccgtcgcgtt ttggtacatg agcctgatcg gcgggctcat cacgctcgcc tatgcgatct
     1201 atcgaaaaga tccggtcttc atcgcaggac agagcatcgg gtctctcgtc tatattcgca
     1261 acctgatgct gattgcacgc gcgagccagg cgagccagcc ggccagcccc tctcctccac
     1321 gaacataact ctttgagatc atacattctc aacgtatgcg gtcagcacac gctccgaaca
     1381 cttctatgat cacgaccgac cgttcacatc atgctgtact ccttctggcc ctcctcctgc
     1441 tctcgggact gttgttcttc ttgggactcg gcgagatggg cctgaccgac caggacgagg
     1501 gacgaaacgc cgaggccggg cgtgaaatgt tcgaatcggg ggatcatctc accccgaggt
     1561 tcaacggtga gctgcgcgtg gcaaaaccag tcttgatcta ttggttgatg gaacaatcgt
     1621 atcgcctgtt cggtgtcaat gagtttgccg cacgattccc ctcagccctc ttcggcgtgg
     1681 gcttgatcct gatccattac ctgtttcttg tacaccagcg tgatcgaacc gtcgcactct
     1741 tcggcggctt gatgctctta ctcaatctgg aaatccttgg gctcggacgc atggcgctga
     1801 ccgacagtgt cttgattttt ttcacgacgg cctcgttgta cggattttgg cttggactgc
     1861 atggagaagg cgtcgtacgc cgatggatgt ggatgttcta tatcgggatg gccctcgcca
     1921 cgttgacgaa gggaccggtc gggttcgccg tgcctctcat cgcggcggcg ctgtacctca
     1981 cctggacgcg ccggtggcgg gactattggc agaaggggat gccccttgcg ggtatgctgc
     2041 tgttcgcgct gctcgccgca ccctggtatg cggccatgtt gtacgtgcat ggcgacgcct
     2101 atgcttcagc cgcgaagacc aacaccattg gccgattcct cagtccgatg gaagggcatt
     2161 atgggacgat cttcttctat atcccggtcc tgctcgtcgg cttctttcca tggagcgcac
     2221 tgctgcccct cccgctctat cacacattca aagagtggta cctcatacgc cgtgcgcgtg
     2281 ctcgcccgga tcccaccggg acgaccgaac tcgacctttt tgcggctcta tgggtcgtca
     2341 gcgtattcgt gttcttcacg gcctccgcaa cccgcttgcc ccactatatc ggtccgttgt
     2401 ttccagccgc tgccctgttg acggcctcgt actggtcgcg aggcctggac gatccacaca
     2461 cgaaagggat tcgggcctcc attcacctca tgatgggtgt gggatatctt ttggcaatcg
     2521 gcttcgcgtg ccttccaacg ctctacacaa cctatgccga caagatggtc cgggaattcc
     2581 ctttagctgg acaggtcgat ctgggcatcg ggccctatct tgttgcagcc ctgttgttat
     2641 tcggaatgac cttagtgggg tattttggat tgaacgatga acggcgagga gaagcgtttt
     2701 gggcagccgg gggaaccttg gcgggtctag ttctgatcgt cattgtgatc gcgattccac
     2761 accttaaccg atatgcagtc gcaccggcac aggaactcgc ctatgccgca ggactgaacc
     2821 tgaccccaca ggatcaattc atcgcctatg gcaccacgag agcctcgtca gtattttatg
     2881 caagacgcaa gacgctcttc gtgcatcggg gggaagcgga catgatacgg gcggccctga
     2941 aagagcctaa gagagtgatg atcctcttgc cggaaaacgc acaatctaaa ctgccggcgg
     3001 aggcaattgg gctagtcccc attctcaaac gctacgggta tatcctgttg gccaaccagc
     3061 cgatggtgac gattccagaa ggggcacctc cgccacctcc acccaaaata cttggccatt
     3121 aaactggtct gtctcgtctg ttcggtctat ctagtctatc tggtgtctga ttgaaccaga
     3181 cagaccgaac agaccagata gtccggatag accagactac atcggcacga tgccttttag
     3241 ggcataactt gcccccaata ccagcagcat agccgcgacg aacagacctt ccgccatggc
     3301 tgcccataac cccacgggac cctccgtcat gggctcgatc tgaccaggca gattattgag
     3361 ccaataggca gcacaggcga ggagcacaac ggccgtgacc aatagagagc cggttgtcat
     3421 ccctaacccc agacccacga gaccgcgagt gagggaaccc gtcagccaag ccgggcgcca
     3481 tgacaacgac tgcaccaacc acaagacatg gcccacgagt cccgccaggg taaggaacag
     3541 gccaccccat acgagctgtt gaaacggcca tgcttctgag ggaaggtgca cgatcgtcca
     3601 gacgaaggcg agcgtggcga caaagaacgg ggttatccgg cagacagccg tcccaatcga
     3661 tgcgcgccat tcacgccacg gatgggctgc aatcattccc atgacacagc caagggccgc
     3721 tcctcctgcg gcgtcggtga gatagtgaga ccctcggaca atccgactgg cggcaatgac
     3781 tgctgccaca gcaagaatgg gccatttcgt gcgcgggaac ttgatagcca gcacggttgc
     3841 gaccgcgaac gcagccgacg catgcccgga cgggaatgag tcccatccgc tgccgctggc
     3901 cggggaaaat tccagattgc cggcatgcat gaacttggga cgggggcgac caatcatatg
     3961 tttcaaaata ttcacgctca acgcggcaag accgtgcgcg ataagactct gccagcctgc
     4021 ctctttccat tggggagttt tgaatccata cccgaccgcc agcagcacga gactcagaat
     4081 cacaagagac tccccgctgc cgagcaggtc gccgaaatca ctgagctgag ccagccaggg
     4141 attggcaagg tggtccctgt gaacatcgtt gagggaacgg atgaacctcg tcagcggtac
     4201 gtcccactca aacaaccccg cgaatgtcag tatgagcgca cagacggaga ggaggaaggc
     4261 ggcaacaggc acgaatcgtg agctgtgaat cgtgtctcgc gggagcgcca tgagaatcga
     4321 tctacacgtt tagcgacgaa caatatgagc aggatgctca aaacgtcggt ccggcaagac
     4381 cgcagcgagt gaagagggaa ggaggtatgt accgcacatc gtgtgcggcc gttcgccctt
     4441 acaataggtc ttggccaacg gaaaagcccc tctagtgttt cccgagaaac tcaatgttga
     4501 gcctctgaac gatgcgagaa cgatgctggc gggctttttc agcattctgc tagggaaccc
     4561 agtcggcgag gaagacattg aattctccag gcgtcttggc cttccgatcc gatacccaga
     4621 ccaggctggt accatccggt gagaacatgg ggaaactgtt gaattgcccg tcgaacgtga
     4681 gccgctcaag accttgcccg tcctcaccga tcgcatagag gtgaaagctt ggtcggcctc
     4741 cttcgccttt cgtttcaata ttcgatgaaa aaatgaccct cttgccatcc ggaaaataga
     4801 agggcgaaaa attcgatgcc ccgttcgacg tgacggctcg cttaccgctt ccatcggcat
     4861 tcatcacgaa gatttcaagt tgtccaggct ccaccaaccg ctggttgagc aggtccttgt
     4921 acttgtcaat ctccgcctga tcggtaggat gtgaagcccg gtacacgata cgcgtgctgt
     4981 ccggggaaaa gaacgctccg ccgtcatagc cgacctcgtt cgtcaaccgc cggggcttcg
     5041 agccatcgag attcatggca tggagatcca aatccccgtc tttcacggat gtccagacga
     5101 tcgtcttgcc atccggcgaa atagtcgctt ccgcatcgta gcccggcgtg aaggtcagcc
     5161 gctgcatttc ttgaccatca atgcctacgg caaaaatatc gtagtcgtcc aacgcccatc
     5221 ggtaggcccc gtcccgtttt ggcttcggcg gacagtttgg ccccgacgca tgggtggacg
     5281 aatagaggat gcggcgatcg cccgggaaga agtaaccgca cgtcgtggca cccgcgccgg
     5341 tactcaccaa tcggacggtg ccgctgtcca gatccatcac atacatctga taacaaccca
     5401 gcccggcgtc ggccggtttg agcg
//
LOCUS       DATIPF010000398         3098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_250148, whole genome shotgun sequence.
ACCESSION   DATIPF010000398 DATIPF010000000
VERSION     DATIPF010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250148"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VLL06_09985"
     CDS             complement(<1..581)
                     /locus_tag="VLL06_09985"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31335.1"
                     /translation="MRTDLKSGLIKTVAGTGESGWTGDGSEALAACLNEPKGLTLDIE
                     GNLFIADSENHIVRKIDKKTGSIRTVAGRIQSAESGTTPASPVPVAQRGDDDPFAEGG
                     TVHPHAFSQQTDLSGTVRYVVTGTAPAKRFSGDGGPADQALLNFPTAVVVDRVGHLYI
                     ADTLNHRVRRVEAVTGIITTVAGLGQPRCSGDGG"
     gene            complement(600..1673)
                     /gene="leuB"
                     /locus_tag="VLL06_09990"
     CDS             complement(600..1673)
                     /gene="leuB"
                     /locus_tag="VLL06_09990"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HSS31336.1"
                     /translation="MKAKIAVLAGDGVGREIVPEAVKVLKAVAEKFQHAFEFLSGDVG
                     GQAIDKVGVPLPQETLTLAKQSHAVLLGAVGGPKWEGLEYSLRPERALLGLREHLGLY
                     ANLRPARLYPMLADASTLKREVIEGIDILVIRELTGGIYFGKPKGIEKLPNGSERGVN
                     TEVYTTEEIQRIAKVAFEAARKRRKKVMSVDKANVLESSELWRRVVTDVHKGYRDVEL
                     SHIYVDNAAMQLVRNPRQFDVMLCNNIFGDILSDEAAMLTGSIGMLPSASIGAQVGLF
                     EPIHGSAPDIAGKNVANPIATIASAAMMLSYAFKLDKEAAAIEQAIVKTLDLGFRTKD
                     IHSPGTRLVTTTEMGEAILRNLN"
     gene            complement(1713..2075)
                     /locus_tag="VLL06_09995"
     CDS             complement(1713..2075)
                     /locus_tag="VLL06_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSS31337.1"
                     /translation="MVHVTLADRPDFLAGDHTVLRELLHPGKQPVALGYSMAHGILAP
                     GARSKWHRLASSEVYYIVAGRGRLTVDDSVVAVEPGSVVYVPPGGKQSLENSGPTAIE
                     FLCLVDPAWRSEDEQVLE"
     gene            complement(2148..>3098)
                     /locus_tag="VLL06_10000"
     CDS             complement(2148..>3098)
                     /locus_tag="VLL06_10000"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HSS31338.1"
                     /translation="SVHCHNDLGLAVGNSLAAVFEGAGQVECTINGIGERAGNTALEE
                     IVMGLRTRKDFYQADTKIRTEEISKTSRLVSKITGMVVQPNKAIVGANAFAHTSGIHQ
                     DGLLKDKTTYEIIRPETIGLIESKMVMGKLSGRHAFRQRLVELGYSLSDEEVNHAFER
                     FKKLADQKKEIYEEDLEVIVSEELAKMADRITLKSFHVASGTSQVPTATVELEIDGHT
                     ITQTGTGDGPVDAVYRTIAAITETKSTLLMYVVKAITGGTDAQGEVSVRVQEEGRTVS
                     GHGADTDIITASARAYLNALNKLSYLAGKQAQGEHNVRLI"
BASE COUNT          652 a          996 c          739 g          711 t
ORIGIN      
        1 cccccgtcac ctgagcaccg aggttggccc agacctgcga ctgtcgtgat gatccctgtt
       61 accgcttcca cacgccgcac ccgatggttc agcgtgtccg caatgtagag gtgccctacc
      121 cgatcgacca caaccgcagt cggaaaattc agcagcgctt gatccgcagg accaccgtcg
      181 ccgctgaagc gtttggccgg tgccgtaccg gtcacaacat accgcacggt tccactcagg
      241 tcagtctgtt ggctaaaggc atgcgggtgg accgtgccgc cttcggcaaa aggatcgtca
      301 tcccctcgct gagccaccgg aacgggcgag gcgggtgtcg tccctgattc agcactctga
      361 atgcggcccg ctaccgtcct gatacttccg gtcttcttgt caatcttccg aacgatgtgg
      421 ttctcagagt cggcaatgaa caagttcccc tctatatcaa gagtgagacc cttaggctca
      481 ttcaaacaag cagcgagggc ctcagacccg tcaccagtcc acccggattc tccggtaccg
      541 gcaacagtct tgatcaatcc agatttgaga tctgtcctca tccgtttcga caatcctggc
      601 tagttcagat ttctcaggat ggcctcaccc atttctgtcg tcgtgacaag gcgagtaccg
      661 ggactatgaa tatctttcgt acgaaagccg agatcgaggg ttttcacaat cgcctgctcg
      721 atagcggctg cttccttgtc caacttgaac gcataggaca gcatcatggc cgccgaggca
      781 atcgtcgcaa taggattcgc cacattcttc ccggcgatgt ccggcgcgct gccgtgaatc
      841 ggttcgaaca gtccgacctg cgcgccgata ctggccgagg gtaacatgcc gatggatccg
      901 gtcaacatgg ccgcttcgtc gctcagaatg tcaccgaaga tattgttgca gagcatcaca
      961 tcgaactgtc gtgggttgcg caccagttgc atcgccgcat tgtcgacata gatatggctc
     1021 aactccacat cccgataacc cttgtgaaca tcggtaacga cacgacgcca gagttcggat
     1081 gactccaata cgttcgcttt gtcaacggac atcacttttt ttcgtcgctt tcttgccgct
     1141 tcgaatgcca ctttcgcaat gcgctggatt tcttccgtgg tatagacctc ggtgttgaca
     1201 cctcgttcac tcccattcgg cagtttctcg atcccttttg gctttccaaa atagatccca
     1261 ccagtcagtt ctcgaatgac gaggatgtcg atcccctcga tcacttctcg tttcagtgtt
     1321 gaggcatcgg ccagcatcgg atacaacctc gccggcctta aattcgcata gagacccaga
     1381 tgctcgcgca atcccaacag cgcgcgttcc ggacgtagac tatactccag cccctcccac
     1441 ttaggaccgc ctactgcgcc aagcaatacg gcatggctct gtttggccag ggtcagcgtc
     1501 tcctgaggga gcggcacacc tactttatca atggcctgtc ccccaacgtc accggagaga
     1561 aactcgaagg catgctggaa cttctcggcc acagccttca gcaccttcac agcctcaggg
     1621 acgatctccc gacccacccc atcaccggcc agtacggcaa tcttcgcttt cacacaccac
     1681 tctccttctt tgagttaaga ccgcacaagc ctctattcca ggacttgctc atcctctgat
     1741 cgccacgcag gatcgactaa gcagaggaac tcgatggcgg tgggtccact attttctaac
     1801 gactgctttc cccccggagg cacatagacg acggagcctg gttctaccgc gacaaccgag
     1861 tcgtctacgg tcaatcgtcc ccgacctgcg acaatataat agacctcaga ggacgccagc
     1921 ctgtgccatt tcgaccgagc gccgggagcc aatatcccat gcgccatact atacccaagg
     1981 gcgaccggtt gtttccccgg atgcagaagc tctcgcagaa cagtatgatc tccggccaaa
     2041 aaatccggtc gatctgccag ggtgacatga accacaactc acctcacagc cagtaatacc
     2101 gccctactcc cagaccggag ccgctgactc taccgtggtc actttcttta aatcaagcgt
     2161 acattatgct cgccctgcgc ctgttttcca gccaggtacg acaacttgtt caatgcgttg
     2221 agataggccc gagccgaagc cgtgatgata tccgtatctg ccccatgacc tgagacggtg
     2281 cggccttctt cttgaacccg aaccgagact tcgccctggg cgtcagtccc accggtaatg
     2341 gccttcacga catacatgag gagggtgctc ttcgtctccg taattgcggc aatcgtccta
     2401 tagaccgcat cgaccgggcc atcgcccgtc ccggtctgcg tgatggtgtg cccgtcgatc
     2461 tcaagctcga ccgtagcggt tggcacctga gacgtcccgc tagcgacatg gaacgacttc
     2521 agagtgatcc gatcggccat cttggctaac tcttccgaga caatgacttc gagatcctct
     2581 tcatagatct ccttcttctg atcggccaat ttcttaaacc gctcgaacgc atggttcact
     2641 tcctcatccg agagtgaata ccccaactcc accagcctct gccgaaatgc atgccggcct
     2701 gaaagcttcc ccatgaccat cttactttcg atcaacccaa ttgtctccgg ccgtatgatt
     2761 tcataggtcg tcttgtcctt cagcagccca tcttggtgaa tcccagacgt atgggcaaag
     2821 gcattcgccc cgacgatcgc cttgttcggc tgcacgacca taccggtgat cttgctgacc
     2881 aatcggctgg tctttgagat ctcttcggtg cgaatctttg tgtcggcctg atagaagtct
     2941 ttcctggtcc gaaggcccat gacaatttcc tccagcgccg tattccccgc ccgctcaccg
     3001 atcccattga tcgtacattc gacctggccg gccccttcga acaccgccgc caagctgttc
     3061 cctacggcaa ggccgagatc gttgtggcaa tggacgga
//
LOCUS       DATIPF010000399         2918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_166343, whole genome shotgun sequence.
ACCESSION   DATIPF010000399 DATIPF010000000
VERSION     DATIPF010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166343"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1186
                     /gene="galT"
                     /locus_tag="VLL06_10005"
     CDS             182..1186
                     /gene="galT"
                     /locus_tag="VLL06_10005"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HSS31339.1"
                     /translation="MPDLRRDPIVGRWVIITTERSGRPHDFIPVHATRPLAATLCPFC
                     PGQERLTPKEIMAYRLPPVEPNGPNWTVRVVPNKFPALQVEGDIDRQGLGLYDRMNGI
                     GAHEVIIETPNHTDSLADMPTKRIEDVLWAYRDRMIDLKKDVRFRYILIFKNHGASAG
                     ATLEHSHSQLIALPIIPTSVLGEIEGCRAHYEQKERCIYCDILRQDLSDGDRIVAENP
                     EFVCVTPYAPRFPFEMWILPKRHAGYFEESQKLQFEFLAPILSEALRRMDKALARPSY
                     NFILHSSPLHEKTGDFYHWHIEIIPKLTQVAGFEWGTGFYINPVSPEESAKFLREATL
                     "
     gene            1187..2575
                     /locus_tag="VLL06_10010"
     CDS             1187..2575
                     /locus_tag="VLL06_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31340.1"
                     /translation="MPVRFRIEDPLLTQPLRHIEQIVRAAGGRTWLVGGCLRDLVLGQ
                     QPRELDLEISGLPPGQLHALLTAHFSLQLVGKAFGVFRLQGLPIDISIPSRTITDSES
                     APRPLKQPDPDMDIDEALARRDFTMNAMAWDPDTLELRDPFNGRADLHAHVLRHASDR
                     FGEDPTRVLRGMQLAARFGLTAAPETVALCATLTQAGQPRERLWEEWKTLLLHGRRPS
                     LGLQFLSDCGWLRFYPELAALQGCEQDPIWHPEGNVWIHTLHCLDWFAGERIGEHDDD
                     LIVGLGILCHDFGKPATTTTDFGPITSRGHEPEGEAPTRHFLERLTNQDNLINEVIPL
                     VRCHLRPRALHDANASDSAVRRLARQVKRIDRLVRVARADHAGRPPKPFDGFPAGTWL
                     LERARRLEVANQAPTPLVLGRHLLELGIQPGPDMGRLLDDCYEAQLDGIFSSLGDGLT
                     FARQQFSQSRHI"
     gene            2649..2846
                     /locus_tag="VLL06_10015"
     CDS             2649..2846
                     /locus_tag="VLL06_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HSS31341.1"
                     /translation="MHIQLSHPTRTVEVKGPKKVKELLRELDLVVEAHLVIKGDDLVT
                     EDEMLYDGDQVEIRPVISGGN"
BASE COUNT          618 a          926 c          792 g          582 t
ORIGIN      
        1 aatcgcacga tacccgcacc acaagcctgc gaccctcctc gtaccgggtc cagaattcga
       61 agcagatcta tggcgagtgt aatggatatg aaaggtcgaa ggtgacatgt gaaaggcggt
      121 cttgggcacg tactcccaga aaccttttac gtgttacgtg tcatgtgtaa cggaggaaac
      181 gatgcctgat ttacgccgtg atccgattgt cggtcggtgg gtgatcatta cgacagaacg
      241 ttccggtcgg ccccatgact ttattcctgt gcacgccacc agacccttag ccgcaaccct
      301 ctgcccattc tgcccgggac aagaacgact gaccccgaaa gaaatcatgg cctaccggct
      361 gccgccggtg gagcccaacg gcccgaactg gaccgtgcgc gtcgtgccga ataagttccc
      421 cgcgctccaa gtcgaaggcg acatcgaccg ccaaggtctc ggcctctacg accgcatgaa
      481 tgggatcggc gcccatgaag tcatcatcga gactccgaac cacaccgata gcctggccga
      541 catgccgaca aagcggatcg aagacgtgct gtgggcctat cgcgatcgca tgatcgatct
      601 gaagaaagac gtacggtttc gttatatcct gattttcaaa aaccacggcg catcagctgg
      661 cgccacattg gagcacagcc actcacaact catcgcactg ccgatcatcc ccaccagcgt
      721 gctcggagag atcgagggct gccgggctca ttatgaacag aaagaacgct gcatctattg
      781 cgatattctt aggcaggacc tctctgacgg cgatcgcatc gtggcggaaa atcctgaatt
      841 cgtctgtgtc actccctatg ccccacgctt tcccttcgaa atgtggattc tcccgaaacg
      901 ccacgcaggc tacttcgaag agagccagaa gttacaattc gagttcctcg cgccgattct
      961 ctccgaagcg ctacgacgga tggacaaggc cctggctcgg ccctcgtaca actttatcct
     1021 ccatagttcc cccctccacg aaaagacagg ggatttctat cattggcaca tcgaaattat
     1081 tccgaagctg acccaagtag ccggcttcga atggggcacc gggttctaca tcaacccggt
     1141 gtcaccggag gagtcggcga agtttcttcg ggaggcgacg ctgtaggtgc cggttcgctt
     1201 tcgcattgaa gatcctttgc tcacgcaacc gctgcgccac attgaacaga tcgttcgcgc
     1261 ggctggcggc cggacatggc tggtcggcgg ttgcctccgc gacctcgtgc ttggtcagca
     1321 accgcgcgag ctggaccttg aaatttctgg gttgccgccg gggcagctcc atgcgttgct
     1381 caccgcgcac ttctccctgc agctcgtggg gaaggccttc ggtgtgttca ggctgcaagg
     1441 cctgcccatc gatatttcca ttccctcccg cacgatcacc gacagcgagt ccgccccgag
     1501 accgctgaaa caacccgatc ccgacatgga catcgacgag gctctggcgc gacgcgattt
     1561 caccatgaac gccatggcct gggaccccga taccctggag ctccgcgatc cattcaacgg
     1621 tcgagctgat ctccatgccc atgtgctgcg ccacgcttcg gatcgatttg gcgaagatcc
     1681 cacgcgtgta ctacgcggca tgcaactggc tgcacgattc gggttgactg ccgcgccgga
     1741 aacggtggcg ctctgtgcaa cactgacgca agccgggcaa ccgcgcgaac ggctctggga
     1801 agaatggaag acacttctcc tccatggaag gaggccctca ctgggtctgc agttcttgag
     1861 cgactgcggc tggctccgct tctacccgga actcgcggcg ctccaaggct gcgagcaaga
     1921 tcccatctgg cacccggaag gcaatgtctg gatccacaca ctccactgct tggactggtt
     1981 cgccggggaa cgcatcggcg aacatgacga cgatctgatc gtcggcctcg gcatcctctg
     2041 tcacgatttc ggcaaacctg cgacgaccac gaccgacttt ggacccatca cgtcgcgcgg
     2101 ccacgaaccg gaaggagagg ccccgacgag acactttctc gaacggttga cgaaccaaga
     2161 caatctcatc aatgaggtga ttcctcttgt gcgctgccac ctccgcccac gtgcgctgca
     2221 tgacgcgaac gcctctgaca gtgcggttcg ccgactggcc cggcaggtca aacggatcga
     2281 ccggcttgtg cgcgtcgcgc gggccgatca cgcaggccgc ccccccaaac cctttgacgg
     2341 atttcctgct ggtacctggc tgctggagcg tgcgcgcaga ctcgaagttg cgaaccaggc
     2401 ccccacccca ctcgtcctgg gtcgtcacct actcgaattg ggaatccagc cgggccccga
     2461 tatgggccga ctgctcgatg actgttacga agcgcagctg gatggaatct tctcctcgct
     2521 cggcgacggg cttaccttcg ctcgacaaca gtttagtcaa tcccgtcaca tctagtctcc
     2581 tgtccgcaca tccatcactc atcaccacgc cccttgcccc ctgctttatg gaagcttatg
     2641 attcccccat gcacattcaa ctgagccatc caacaaggac cgtcgaggtc aagggtccca
     2701 agaaggtcaa agaactcttg cgtgaattgg atctcgttgt cgaagcccat ctcgtcatca
     2761 aaggcgatga tctcgtgact gaagacgaga tgctgtacga cggcgatcag gttgaaatca
     2821 gaccggtgat ctccggtgga aattgaagag ggcgaggggt cagaggctag gggctagagc
     2881 aaaccgattc tcagtcccct tgcctctcgc cacgcgcc
//
LOCUS       DATIPF010000400         4055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1228824, whole genome shotgun sequence.
ACCESSION   DATIPF010000400 DATIPF010000000
VERSION     DATIPF010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228824"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="VLL06_10020"
     CDS             <1..1201
                     /locus_tag="VLL06_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HSS31342.1"
                     /translation="KPIEQQLTEGQELMVQISKGPIGTKGSRVTTYVSLPGRYLVFMP
                     NVEHIGVSRRIARDEERARLKDIMKRVRHPGCGYIVRTVSEGVKEDELRSDVDFLHVL
                     WQDILAKREQKGSPALLHADLSLSFRVVRDLFGKKVDRLWIDSREEYQAVRDFVQRFS
                     PEQTSRIHLYDKDESLFDHLGVEQEMARALSRKVWLKSGGHLVIDHTEAMTVIDVNTG
                     RFVGKRDQEETILRNNLEAAKEVAYQIKLRGIGGIIIVDFIDMEREKNRDKVYHALLD
                     AMSTDKARTRVSRISDLGLIEISRERVREDLLRSLSEPCLYCEGRGYTKSPTTVAYEI
                     FRDVRRIGSSPEPQRIVIGAHPSVVGLLQDEERQGLEAVERECAAKIIVMPDEHLHLE
                     QYDLAVL"
     gene            1303..2421
                     /gene="dprA"
                     /locus_tag="VLL06_10025"
     CDS             1303..2421
                     /gene="dprA"
                     /locus_tag="VLL06_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HSS31343.1"
                     /translation="MTRAELESWFCLRAIDGVGDLTVLRLVRTWHSPGAVLRASHDEL
                     IQRGCSLQLADAIRRGPDRSAHRGIETELNAIERRCIEVRSVLDPTYPTRLKMIADPP
                     PLLYIRGTLTEQDDLAVAIVGARRATASGRALTEELSHDLAEAGMTVVSGLARGVDAA
                     AHRGALAAQGRTIAVLGCGIDRTYPPEHARLRGQIEERGAILSEVPVGSPPHSHHFPR
                     RNRIISGLSLGVIVTEAAISSGSLITAKLAAEQGREVFAVPGFVKEDTSRGTNALLKD
                     GAALIERAQDVIDVVMAQLEPALRLRLQSSREKRVPGDQLGKEEQRVYDVLSYDPRTV
                     DEVIVSTGLSVSTVMASLLSLELRQRIRPLPGQRYLRV"
     gene            2496..>4055
                     /gene="topA"
                     /locus_tag="VLL06_10030"
     CDS             2496..>4055
                     /gene="topA"
                     /locus_tag="VLL06_10030"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HSS31344.1"
                     /translation="MAKALIIVESPTKARTISKYLGRGYTVMASVGHIKDLPTSKLGV
                     DLEHDFTPQYVTIKGKAKVLADIKKKAEEVDKVFLAPDPDREGEAIAWHLEQELLGKS
                     KSKSKKKTEGKVFRVLFNEITESAIKRALQSPGQVDMKLVNAQQARRVLDRIVGYQGS
                     QLLWTKVRRGLSMGRVQSVAMRLICEREQEREAFRAEEYWSIAGLLSGTNPPPFEAKL
                     HSINGQDASIATETEARRIVEAIQGKEFVVDSIERKEKRRNPVAPFITSRLQQEAARK
                     LHFSSKKTMTLAQKLYEGIEIGAEGPTGLITYMRTDSPRISNEAMAEARQVIQDRFGA
                     EYLPATPNVYKTQKAAQEAHEAIRPTSAARDPESIRQYLEPDIHKLYQLIWNRFIASQ
                     MVPAILDVTRVESSPVMTKGKFLFRSTGTVVKFPGHTIVYMEGIDKELFAQKQKGEQE
                     VEDDAERQLPLLSEGERLRLVSQEGETVPGVLSKQHFTQPPPRYNEALLIKELEEKGI
                     GRPSTYASIIST"
BASE COUNT          930 a         1048 c         1262 g          815 t
ORIGIN      
        1 caagccgatc gagcagcagc tgactgaagg gcaggaactg atggtgcaaa tttccaaagg
       61 gcctatcggc acgaaaggct cgcgagtcac gacctatgtg tcgctccctg gccggtatct
      121 cgtgttcatg ccgaacgtgg aacatatcgg tgtctcccgc cgtattgccc gcgacgaaga
      181 gcgggcacgg ctcaaggaca tcatgaagcg agtgcggcat ccaggctgcg gatatattgt
      241 ccgcaccgtg agtgaagggg tcaaagaaga tgagttgcga tccgacgtcg attttttgca
      301 cgtgctgtgg caggacatcc tggccaaacg tgagcagaag gggtcgccgg cattgctcca
      361 tgccgacttg agcttgagtt tccgcgtcgt gcgcgatctc ttcgggaaga aagtggatcg
      421 gttatggatc gattcgcgtg aggagtacca ggcggtcaga gattttgtgc agcgattctc
      481 gccggagcaa acgtcgcgca ttcatttgta cgacaaagat gaaagtcttt tcgatcatct
      541 gggcgtggaa caggaaatgg cgcgggccct cagccgcaaa gtctggctca agtccggtgg
      601 ccatctcgtg atcgaccata cggaagcgat gaccgtcatc gacgtcaaca ccggacggtt
      661 tgtggggaag cgcgatcagg aggagaccat tctccggaac aatctggagg ccgcgaaaga
      721 agtggcctac cagatcaagt tacgcgggat cggtggcatc atcatcgtcg attttatcga
      781 catggagcgg gagaagaatc gtgacaaggt ctaccacgca ttgcttgatg cgatgtcaac
      841 cgacaaggcc cgcaccagag tgtccagaat ctccgacctg gggctgatcg agatttctcg
      901 tgaacgggtc agagaggacc tcctgcggtc actttccgaa ccctgtctct attgcgaagg
      961 gcggggctat acgaaatcgc cgaccacggt ggcttatgaa atattccgcg atgtccgtcg
     1021 gatcggtagc agtcccgaac cgcagcggat cgtcatcggc gcccacccct cggtcgtcgg
     1081 gctgctgcaa gatgaagagc ggcaaggcct tgaggctgtg gagcgtgagt gcgccgcgaa
     1141 aattatcgtc atgccggatg agcacttaca tttggaacag tatgatctag cagtgctcta
     1201 aggattggtg gtttctgtgc gacaagatct atgcagcttt ccagccagtc tgtaggtacg
     1261 gactcctcag ctgaggtcgc aggtccttct tccggccatc gcatgacaag ggcggaactg
     1321 gagtcgtggt tctgcctgcg agcgatcgat ggagtggggg acctgactgt ccttcggtta
     1381 gttcgcacct ggcattctcc tggggcggtc ctgcgggcct cccatgatga gttgattcag
     1441 cgtggatgta gcctgcaact ggccgatgcg atacgccgtg ggcctgatcg ttcagcccat
     1501 cggggcattg aaacggagct caatgcaatc gagcgtcggt gcattgaagt ccggagtgtg
     1561 ttggatccca cctatcccac acgactgaag atgattgcgg accctccacc gctcttgtac
     1621 atcaggggca cattgacgga gcaggatgac ctggcagtgg ccatcgtggg tgcacggcgt
     1681 gcgacagcgt ccggacgtgc cctgaccgag gagctgagcc acgacttggc agaggccggc
     1741 atgacggtgg tgagtgggct tgcgcgcgga gtcgatgcgg cggctcatcg aggcgcgctt
     1801 gccgcgcagg ggcgtacgat agcggtgctg ggttgcggga tcgatcgtac ctaccctccg
     1861 gaacatgcaa ggttacgcgg acagatcgag gagcggggag cgattctgtc cgaagtgccg
     1921 gtaggttcac ctcctcacag ccatcacttt ccgcggcgta atcgtatcat cagcgggttg
     1981 tctctgggtg tcattgtgac agaggcggcc atcagcagtg gatcattgat taccgcgaaa
     2041 ctggcggccg agcagggccg agaggttttt gcggtaccag gatttgtgaa ggaagatacc
     2101 agtcgcggaa cgaacgcatt gctcaaagac ggtgcggcgt tgatcgagcg tgcacaggat
     2161 gtcatcgatg tagtcatggc gcaattggaa ccggcgctgc gtctgcgcct gcagtcctct
     2221 cgagagaaac gcgtgccagg cgatcagctg ggcaaagaag agcagcgggt gtatgatgtt
     2281 ttatcgtatg acccgcgcac agtagacgag gtgattgtca gcactggatt gtcggtgtcc
     2341 acggtgatgg cttcgctctt gtccctggag ctccgacagc gaatcagacc gctgccgggg
     2401 caacgctacc ttcgagtgta atcatcgaac agttgcacag atgcggatta tttgatacgg
     2461 atgagagggt aacctcgtag cgaaccggag tcttcatggc caaagcactc ataattgtcg
     2521 agtccccgac gaaagcacgc accatcagca agtatcttgg gcggggttat accgtcatgg
     2581 cttcggtcgg ccatatcaag gatctcccca cgagcaagct gggcgttgat ctggaacatg
     2641 atttcacgcc gcagtatgtg acgatcaagg gaaaagcgaa ggtattggcg gatattaaga
     2701 aaaaggcgga agaggtcgat aaggtcttcc tcgcgccgga ccctgaccga gaaggcgaag
     2761 cgatcgcgtg gcacctcgag caggagttac ttggaaaatc aaaatccaag tccaagaaaa
     2821 agacagaggg caaggtcttc cgggtactgt tcaatgaaat cacggaatcg gccatcaagc
     2881 gcgcgctgca gtcgcctggg caggtcgaca tgaagctggt gaatgcgcag caggcccgcc
     2941 gagtgcttga ccggattgtc ggataccagg ggagccaatt gctctggacc aaggtgcgcc
     3001 gaggactcag catgggccgt gtccaatctg tcgcgatgcg attaatttgc gaacgtgaac
     3061 aagaacgaga agcctttcgc gctgaagagt actggtccat tgcggggctg ttatctggaa
     3121 ccaatccgcc tccgttcgaa gccaagctcc acagtatcaa tggacaggac gcctcgattg
     3181 cgacagagac cgaggcgcgg cgcatcgtcg aggccattca ggggaaagag tttgtcgtcg
     3241 actcgatcga acgaaaagaa aagagacgga atcctgtcgc acccttcatt accagccggc
     3301 tccaacagga agcggcgcgt aagctgcatt tctcatcgaa aaaaacgatg actctggcgc
     3361 agaagctgta cgaagggatc gagatcggag cggaaggtcc gaccggtctg attacctaca
     3421 tgaggacgga ttctccgcgt atttcgaacg aggcgatggc ggaggcgcgc caggtgatcc
     3481 aggaccggtt tggcgcagag tatttaccgg ccacgccgaa cgtgtacaag acgcagaaag
     3541 cggcgcaaga agcacatgaa gcgattcgtc ccacgtcagc ggcccgcgat cctgagtcga
     3601 tcaggcagta cttagagccg gatatccata agctgtatca gttgatctgg aatcgattta
     3661 ttgcgtcgca gatggtccct gcgattctcg acgtgacccg cgtagagtcg agtcccgtca
     3721 tgacgaaggg gaaatttctg ttccgatcaa ccggcaccgt ggtgaagttc cccggccata
     3781 cgattgtcta tatggagggg atcgacaagg agctctttgc gcagaaacaa aagggcgaac
     3841 aggaagtcga ggacgacgct gagcgccagt tgcccctcct cagcgaggga gagcggctgc
     3901 ggctcgtctc ccaagaggga gagacagtcc ccggcgtgct ctcgaagcaa cacttcaccc
     3961 agcctccacc tcggtataac gaagctttgc tgatcaaaga attggaagaa aaaggcatcg
     4021 gcagaccatc cacctatgcg agcattattt ctacg
//
LOCUS       DATIPF010000401         1582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1229060, whole genome shotgun sequence.
ACCESSION   DATIPF010000401 DATIPF010000000
VERSION     DATIPF010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229060"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VLL06_10035"
     CDS             <1..597
                     /locus_tag="VLL06_10035"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSS31345.1"
                     /translation="KVEWGNFSTNFYMILSPGSIEGAPFTYVATVRVPPEKEVPLQQA
                     VVASFPNISAIHIGDVLDGFARVLDRLSLAIRAVALFCVVAGGLVMAAALASTRYRRL
                     YESVILKALGATRGLIARAFAMEYVLLGAVAGLIGLTLGSVLSWAVLRYVFELPWNIH
                     PQVLGIGLFLTMLLTLIVGFASTYRILGQRPLAVLRHE"
     gene            complement(826..1320)
                     /locus_tag="VLL06_10040"
     CDS             complement(826..1320)
                     /locus_tag="VLL06_10040"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS31346.1"
                     /translation="MRTGCCPKCGTLKTKLAPRRGVADRLLGTLTIYPLRCQLCSHRF
                     TTFLGKLKTNPRRNYDRVSVEYPAQVRPIHDPSQSVVVEGTLSNLSLRGCRVRTSQRL
                     PMGSHVMLEFHPAEYDDPIMIDDAIVRSRCAEGIGLRFSSFLRSEERRLRRILELRLP
                     DQAI"
BASE COUNT          336 a          418 c          474 g          354 t
ORIGIN      
        1 aaagtcgagt ggggaaattt ctcgacgaac ttttacatga tcttgtctcc tggctcgatt
       61 gaaggggcgc cgtttaccta tgtggcgacg gtgcgagtcc caccggagaa agaggtcccg
      121 ttgcagcagg cggtcgtcgc ctcattcccg aacattagcg cgatccatat cggcgacgtc
      181 ctcgatggtt tcgcgcgtgt gctcgatcgt ctatcgctgg cgatcagggc cgtggcatta
      241 ttttgtgtgg tggccggcgg attggtcatg gcggcggcac tcgcctcgac acgctatcga
      301 cgtctctatg aatcggtcat tctgaaagcg ctcggcgcga cccgtggcct gattgcccgt
      361 gccttcgcca tggagtatgt cctgctaggt gccgttgccg ggttgatcgg gctgaccctc
      421 gggagtgtct tatcatgggc cgtgttgcgc tatgtgttcg agcttccctg gaacatacac
      481 ccgcaggtgc ttggtatcgg tcttttcctc acgatgttgc tgacactgat tgtgggtttt
      541 gcgagtacct accgtattct cggccaacgg ccgctggccg tacttcgtca cgagtaaaat
      601 tggcgagggg acgaacgacc attagcagac ggcggaaaaa cttggataga cacaaaacac
      661 cgttggaatc ttccagcatg gctccgatgc ctcaacaggt tcaggatgct caaaaaggct
      721 gtccagcaag gccgcagcga gtgaagaccg gaagcgtacc ctcaggggta cgttgaggac
      781 ctgaacgatg cgagaaggaa gctggaggac tttttcagca tcctgttaga ttgcctgatc
      841 gggaaggcgc agttccagga tgcggcgaag gcgtcgttct tcggaacgga ggaaactgga
      901 aaagcgaagc ccgatccctt cggcacaacg ggagcgcaca atggcatcgt caatcatgat
      961 ggggtcatca tactcggcag gatgaaattc cagcatcacg tggctgccca tcggaaggcg
     1021 ctggctcgtt cgcacgcgac acccccgaag agacaaattc gacagtgtcc cttccacgac
     1081 gacgctctgg cttggatcat ggatgggacg gacctgtgcc gggtactcga ctgagacccg
     1141 gtcataattg cggcgaggat tggtcttgag tttgccgagg aaggtggtaa accgatggct
     1201 acagagctga cagcgaaggg gatagatggt cagcgtgcca agaagccggt cagccacccc
     1261 gcggcgtggg gcgagttttg tcttcagcgt tccacatttt ggacaacagc cggtgcgcat
     1321 ggtcggcgga tgactccttg ttgtatcgct ctgcgaacca attataaaaa tcaacacggc
     1381 aaacggcaac cccctccttc gagaggcatg atatagcccg tcaattggga atgaatttga
     1441 acaggcctcc tccttgggtc attacatata gctcgcctgc agcatcttca ccaaagcttg
     1501 tcacggaact gcccggtgga ctgagtaaag gccagtccgt ctggtccgtg ggttgaccat
     1561 tctgatagcg gaagctcttc ac
//
LOCUS       DATIPF010000402         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_872439, whole genome shotgun sequence.
ACCESSION   DATIPF010000402 DATIPF010000000
VERSION     DATIPF010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872439"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VLL06_10045"
     CDS             <1..873
                     /locus_tag="VLL06_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HSS31347.1"
                     /translation="ALRIGPGDEVIMPSYVCPSPWLATKRVGAEARLVDIDLDTFNID
                     PLAACQAMTTRTRAIIVPHLFGLPADLTALERLGIPLIEDCAQTLGATEQGRPVGTVG
                     TVTVCSFYATKLLCAGEGGMVLSNERVLLERVRLLREYDGAPTLDPSSFNLKLTDLQA
                     ALGIRQLGRFQAFQERRGYLAAAFREVLSSKTAVCPSVPPGRTHGYYRFTIRLPHFRT
                     DPDGLPGLINMLEQQGIQCRKPVFRPIHRYLDQSGFPNSDEADRTALSIPLYPDLTDD
                     ELRQIQQSLTKVLS"
     gene            870..2039
                     /locus_tag="VLL06_10050"
     CDS             870..2039
                     /locus_tag="VLL06_10050"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HSS31348.1"
                     /translation="MKAIDVEASPFTLRSHWRWLTPAALGALLGVAALAVPTIRELFV
                     FEGLRWLYILLFAFLGAGALTPLMTHLSHQWGVVDQPSERKIHVHPTPRLGGVAVYLG
                     FLGSVLLNSILPDWMIATLAAGSLLLIVGLIDDVRELPAAAKLAAQLLAAGIVIATGN
                     VLTLFPSGPIGDVANVMLTLLWIVGITNAFNFFDGMDGLATGLAILIAGFMGAVAFET
                     NQPGLGWLAIAIVGAGIGFLPYNFRGRKPALIFLGDGGATFLGFMLACLAVKGNWADH
                     NPIVSFSNPLLIFGVLIYDMVHITVERVATGKVRSIKEWLDYVGKDHLHHRLERALGS
                     RQASVVMIFLLTICLGLAALALRGAGTPEAILLLEQACLIVAMITILEWSGRQKH"
BASE COUNT          419 a          626 c          657 g          539 t
ORIGIN      
        1 gcgctcagga ttggacccgg tgacgaagtc atcatgccga gctatgtctg tccgtcgccc
       61 tggctcgcga caaaacgagt gggggcagag gccaggcttg tcgacatcga cttggatacg
      121 ttcaacatcg atccccttgc agcctgccag gcgatgacca ccaggactcg cgcgattatt
      181 gtcccgcatt tgttcgggct cccggctgat ctcacggcac ttgagcggtt gggaattccg
      241 ctgattgagg actgtgcgca gacgttaggc gcgacggaac agggtcggcc tgtcggtacg
      301 gttggaaccg tgacggtatg ctccttctat gctacgaagc tgctctgtgc aggagaaggc
      361 gggatggtac tttcgaacga gagggtgttg ctcgaacgag tcaggttgct acgcgaatat
      421 gatggcgcac cgacactgga cccatcctcc ttcaatctta agctgacgga cttgcaagca
      481 gcgctgggga tccgccagtt gggtcggttc caggcttttc aggaacggcg cggctatctc
      541 gcggcagcct ttcgagaagt cctgtcctcc aaaaccgccg tctgtccgag cgtgccacca
      601 ggcaggacgc atggatacta tcgatttacc attagactcc cgcatttccg gaccgatcct
      661 gatggcctgc cgggtctcat caacatgttg gagcagcagg ggattcaatg ccgcaagccc
      721 gtctttcgac caatccaccg ctatctggat cagtccgggt tccccaacag cgacgaagcg
      781 gacagaacgg cgctttccat tccgctctac cccgatctca cagacgacga actccggcag
      841 attcaacaat cattgaccaa ggtcctttcg tgaaagccat tgacgtcgag gcctcaccgt
      901 tcacattacg cagccactgg cgctggctga caccggctgc gctgggagcg ttgctgggcg
      961 tggctgcctt ggcggttccc acgattcgtg aactgttcgt gttcgaaggt cttcggtggc
     1021 tctacatcct tctctttgcg tttctcggtg ccggtgcatt gacgccactc atgacgcatc
     1081 tcagccatca gtggggggtg gtcgatcaac catcggagcg taaaatccat gtgcatccaa
     1141 ctcccagact cggtggtgtg gcggtctatc tcgggttcct cggttcggtg ctgttgaatt
     1201 cgattctgcc ggactggatg attgccacac tggcagcggg atcgctatta ctgatcgtcg
     1261 gtcttatcga tgacgtccgg gaactgcctg ctgcggcgaa gttggccgcc cagcttctgg
     1321 ctgccgggat tgttatcgcc acaggcaacg tgctgacctt gtttccgagc ggtccaattg
     1381 gcgatgtggc gaatgtgatg ctgacattgc tctggattgt cgggatcaca aacgcgttca
     1441 acttttttga cggcatggat ggcctggcta cggggctggc gattctcatc gccggattta
     1501 tgggggcggt ggcctttgaa acgaaccagc ctggattggg ctggctggcc attgccatcg
     1561 tgggagcggg tataggattt cttccctaca attttcgcgg gcgtaagccg gcattgatat
     1621 ttcttgggga tggtggggca accttcctcg gcttcatgct ggcctgtctc gccgtgaaag
     1681 gaaattgggc ggatcataat cccatcgtct cattcagcaa cccgctcctc atcttcggcg
     1741 tgctcatcta tgacatggtc cacattactg tggaacgtgt ggcgacgggg aaagtgcgtt
     1801 ccatcaagga atggctcgac tacgtgggca aagaccacct tcatcatcgg ttggaacgcg
     1861 cgttggggtc tcgtcaggcg agcgtcgtaa tgatctttct cctgacgatt tgcctggggc
     1921 tggcagcctt agctcttcgt ggtgccggaa cccccgaggc gatattactg ctcgagcaag
     1981 cctgcttgat tgtggccatg atcacaatac tcgagtggag cggccgacag aagcattaag
     2041 ccttccccct tgcactgtat ctcaattccc gctacaagct tcttctatgg ttcccaccgt
     2101 tgaatggaaa gacggcgcag tacgtctgct tgaccagagc cggcttccgg agtatgtcga
     2161 atttctggat tgccgggact atttgacggt tgccgacgcc attcgcagcc tcaaagtgag
     2221 aggcgctccg gcgatcggcg t
//
LOCUS       DATIPF010000403         4537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_77030, whole genome shotgun sequence.
ACCESSION   DATIPF010000403 DATIPF010000000
VERSION     DATIPF010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77030"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VLL06_10055"
     CDS             <1..1072
                     /locus_tag="VLL06_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HSS31349.1"
                     /translation="VARTSGVSRLSTLWRVTLPLMRPSIAAGVALVILYVVSDFGAVS
                     LLRYQTLTYAVFQQMTGRSDNTAASILSLLLVVFALIFLITERWFRQRSRFYQTTGRY
                     RAPQRQRYGWFGTLAVTSYLGLIVGAAFILPTALLIQWSLSPEAQATVDSRFFGFIWN
                     SAILAAGAATGGVLIGLPLAYLASRRPTMLNLSCLQAAYAGYVLPGPVAALAVLVLFT
                     KLAPVFYGSVLILIVAYIIHFLPAGLQSLEPALQHMSPNLEEVSRTLGLGIRETWRRV
                     TLPLIQNGFVVAWVLMFLQTMKELPATLLLRPVGFDTLAIRVWMEASEEYFQLAAPSA
                     LLIVLLSLPALALLVSKDWRAA"
     gene            1092..2255
                     /locus_tag="VLL06_10060"
     CDS             1092..2255
                     /locus_tag="VLL06_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSS31350.1"
                     /translation="MTSDHSTKPLPSLKSTFQGNLYTPASSVLELREVSCAYEIGRPA
                     VQGISFAAREGEILCLLGPSGCGKTTILRAIAGFEPVRSGQLFLSGQLMSSPDVMIPT
                     ENRRVGMVFQEYALFPHLRVQDNIAFGLRHLVRSERTSRVQGMLRLTGLEGFERRYPH
                     ELSGGQQQRVALARALVQDPVVLLLDEPFSNLDPDMAGRMRQEVHDLLRRTKTTTLLV
                     THDHDEAFAMADRIAVLNQGRLEQFDTPEMMYHMPATPFVADFVGQADFIPGTVSQGM
                     VHTELGEFPDTLECKDGTAVVVMIRPDDIHLIPTKDARSRILSRQFRGSENLYTVSLP
                     SGQIVHSSQGSTSVYQAGTSVELRVMATHTVLFRREDCPGEVSTMVGPIVGEH"
     gene            2348..2779
                     /gene="exbB"
                     /locus_tag="VLL06_10065"
     CDS             2348..2779
                     /gene="exbB"
                     /locus_tag="VLL06_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HSS31351.1"
                     /translation="MNALQTVIDYGIIGFLLALSVWTVAIAVERWLFYRRVDLAQYPN
                     SVGLEMALTKHLVVIGTVAANAPYIGLLGTVLGIMLTFHTMGTSGTMAVNAIMIGLSL
                     ALKATAVGLFVAIPCVVMNNVLRRKVAELLALHKEHHATRH"
     gene            2763..3137
                     /locus_tag="VLL06_10070"
     CDS             2763..3137
                     /locus_tag="VLL06_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HSS31352.1"
                     /translation="MQRDIDQINVIPLVDVMLVLLVIVLTTATFISSGQIPVNLAKAK
                     EVGTRKDAPIIITLTADGALFLNDRTVPEGGLPGALTAEPRESAVVVRADKVTLLERF
                     VSVVDEVRGLGFQQVSLEVIRL"
     gene            3245..4075
                     /locus_tag="VLL06_10075"
     CDS             3245..4075
                     /locus_tag="VLL06_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HSS31353.1"
                     /translation="MKQVPLVPHDEAFTWDVAMMSPGQPVQPAAAPQNHGPTPFVPST
                     TSTPSPPMQQSAPAQTLPPPQPLAQQTTSSIAEEIITPVLPDVRPPQPTTHSQSETRT
                     TPSAKPIAHKPETAMVAESTSIVKQDEAQEGTIAESAAQTVPSNHPSEIPEQASHADM
                     VPAPMQMAAISPAPTGAPAKRDFGWLSEAILRRVEELKRYPASARVDRAEGKVVVKAV
                     INEDGNVGEVEVFQSSGHPGLDKAAIDTLRQAAPFHLPRPLGQPGMTIKIPMSYRLDR
                     "
     gene            complement(4094..>4537)
                     /locus_tag="VLL06_10080"
     CDS             complement(4094..>4537)
                     /locus_tag="VLL06_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HSS31354.1"
                     /translation="ALSGDIKTFGHLPNGKMFPVGIQHPREDQSVLVWVELQDEAIST
                     AGDYERFFERDGVRYHHILDPRVLQPARSCQSVTVIAREGVWADGLDTGIFVMGPERG
                     MELVEQLPDVEAIIVDAKGRLLVSSGLKQRIRLPENPVCRDSLCQ"
BASE COUNT          931 a         1410 c         1230 g          966 t
ORIGIN      
        1 ggtggcacgg accagcggtg tctctcgtct ttcgaccctc tggcgtgtga cgctaccgct
       61 gatgcgtccc tcaattgccg caggagtcgc gctcgtcatc ctgtacgtcg tgtctgactt
      121 cggtgcggtg tcattgcttc gctaccaaac gctcacctat gccgtgttcc agcagatgac
      181 aggacggtcc gacaataccg cagcgagtat cttgagcctc ttgctggtcg tgttcgcact
      241 gatttttctg atcaccgaac ggtggttccg acaacggagc cggttttatc agaccaccgg
      301 ccgttaccgg gctccgcaac ggcaacggta tggctggttc ggcacccttg ctgtgaccag
      361 ctatctcggg ctgatcgtcg gcgcagcctt catactcccg accgcgctct taattcaatg
      421 gagcctgtcg cctgaagcac aagcgaccgt cgacagccgg ttctttggtt tcatctggaa
      481 cagcgccatc ctggccgccg gtgccgccac cggcggcgtg ttgatcggcc tgccgctcgc
      541 gtacctcgca agccgccgcc cgacgatgct gaacctcagc tgcctgcaag ctgcctatgc
      601 cggctatgtg ctcccaggac cggtggcggc actggctgtc cttgtcctct tcaccaaact
      661 ggccccggtg ttctatggct cggtactgat cctcatcgtg gcctatatca tccattttct
      721 tccggccggg ctccaatcgc tggagccggc gctccaacat atgtcgccga acttggaaga
      781 agtctcacgt acgcttggcc ttggaatacg ggagacctgg cgacgagtga cgttgccgct
      841 catccagaat ggatttgtcg tcgcctgggt cttgatgttc ctacaaacca tgaaggaatt
      901 acctgcgacg cttttgttac gaccggtggg gttcgacacc ctggctattc gagtctggat
      961 ggaagcgagc gaggagtatt ttcagctcgc cgctccctcc gcactgctca tcgtcttact
     1021 gagtcttccg gcactggcct tgttagtctc aaaagattgg cgcgccgcct aaagggagga
     1081 acgtagactc cgtgacctca gaccattcca ccaaaccact cccctccctg aagtctacat
     1141 tccagggcaa cctctataca cctgcctcct cggtcttgga actgcgcgag gtgtcctgcg
     1201 cctacgaaat tggacgcccc gcggtccagg gaatttcatt cgcggcacga gaaggggaaa
     1261 tcctctgcct gctcggccct tcaggatgcg ggaaaacgac gattctgcgt gcgatcgcgg
     1321 gatttgaacc ggttcgctcc ggccaactgt ttttgtcggg ccaactcatg tcgtcgccgg
     1381 acgtgatgat cccgacggag aaccggcgcg tcgggatggt ctttcaggaa tatgccctat
     1441 tccctcactt gcgcgtgcag gacaacatcg cctttgggct ccgtcatctc gttcggagcg
     1501 aacgtacgtc acgggtccag ggtatgctgc ggctcactgg gctggaggga ttcgagcggc
     1561 ggtatccgca cgaattatca ggcggccagc aacagcgtgt ggcgctggct cgtgccctcg
     1621 tccaggatcc cgtcgtgctg ctgctcgacg agccattcag caatttggac cctgacatgg
     1681 cgggacgcat gcgccaagag gtacacgacc tcctgcgccg gacaaaaacc acgaccttac
     1741 tcgtgacaca cgaccatgac gaagcctttg ccatggccga tcgcatcgcc gtcctcaacc
     1801 aaggccgctt ggagcaattc gataccccgg aaatgatgta ccacatgccg gccacaccct
     1861 tcgtggcgga ctttgtcggg caggcagact ttattcctgg gaccgtttcc cagggcatgg
     1921 tccataccga actgggagag tttcccgaca cgctcgaatg caaggatggc actgccgtgg
     1981 tcgtcatgat tcggccggac gacattcacc tcatccctac gaaagacgcc cgctcgcgca
     2041 tcctctcgcg ccagttccgt ggctcggaaa atctgtacac ggtcagtctc ccatccggcc
     2101 aaatcgtcca tagcagtcaa ggctccacca gcgtctatca ggcagggact tcggtggaac
     2161 ttcgtgtcat ggcgacccat accgtgctct ttcggcgaga ggattgtcct ggagaggtgt
     2221 ccacgatggt tggaccaatc gtaggagagc attgacagcc tgtttcacga tctggcattg
     2281 atagacgctc acgacggtct cctgccctac cgatccgtga atcactcagt gaaagtgagg
     2341 cctcgccatg aacgcgttac aaaccgtgat cgattatgga atcatcgggt tcctgctggc
     2401 tctcagcgtc tggacagtgg ccattgcggt agaacggtgg ctcttttacc gccgtgtcga
     2461 tctggctcag tatcccaaca gcgtgggctt ggaaatggct ctgaccaaac atctggtcgt
     2521 catcggcaca gtggccgcaa acgcccccta tataggtttg ctgggtaccg tgctgggtat
     2581 catgctcaca ttccatacca tgggcacctc cggcaccatg gccgtcaatg ccatcatgat
     2641 cgggctcagt ctcgcgctaa aagccacggc ggtcggattg ttcgttgcga ttccctgcgt
     2701 cgtcatgaat aatgtgctca gacggaaggt ggccgagctc ctcgccctac acaaggaaca
     2761 ccatgcaacg agacattgat cagatcaacg tcatccctct cgtggacgtc atgctggtac
     2821 tgctggtcat cgtcctgacc acggccacct tcatcagtag tggacagatt cccgtcaatc
     2881 tagcaaaagc gaaagaagtc ggtactcgca aagatgcgcc tatcatcatc accctgactg
     2941 ccgacggcgc gctctttctc aatgaccgca cagttccgga aggaggtctc ccgggtgcac
     3001 tgactgcgga accccgtgaa tcagcggtcg tcgtgcgggc ggataaagtc accctgctcg
     3061 aacgattcgt gtccgtggtg gatgaagtgc ggggattggg atttcaacaa gtcagcttgg
     3121 aggtcattcg tctgtgatcg ccgcgaggcc acatcgaccc ggagtccaga aagacgctca
     3181 cgtccaggga tggctcgtct ccatcttctt gcacggcaca gtagctgcca cagcgattct
     3241 gctcatgaag caggtcccac tcgtgccgca tgatgaggcg ttcacttggg acgtcgcgat
     3301 gatgtcgccg ggacagccgg ttcaaccagc ggcagcaccc cagaaccacg gtccgactcc
     3361 gtttgttccg tcgacgacct cgactccctc tccccccatg cagcagtctg cgccggcaca
     3421 gacgctgccg ccgcctcagc cgcttgctca acaaacgact tcatcgattg ctgaggagat
     3481 cataaccccg gtgctgcctg atgtgcgccc accgcaacca acaacccact cacaatccga
     3541 gactcgtacg acaccatcag ccaagccaat cgctcacaag cctgagacag cgatggtcgc
     3601 cgagtccaca tccattgtga aacaagacga agcacaggag ggaactattg cagaatctgc
     3661 ggcccaaaca gtgccatcga accacccttc agaaatcccc gaacaggcct cacacgctga
     3721 tatggttcca gcgccaatgc agatggcagc catttccccg gcaccaacgg gcgcaccagc
     3781 taagcgcgac ttcgggtggc tctctgaagc gatcttacgt cgtgtggaag aattgaaacg
     3841 ctatcctgcg tcagctcggg tcgatcgcgc cgaggggaaa gtcgtcgtga aagccgtgat
     3901 caacgaagat gggaacgtcg gcgaggtgga agtctttcag agttcgggac accccggtct
     3961 cgacaaagct gcaattgaca cgctgcggca agctgctccg tttcacctac cgcgtccatt
     4021 aggacagccg ggaatgacga tcaagattcc gatgagttac cggttggatc gataggccgc
     4081 tacgggaacg ttcctattga cagagactgt ctcgacatac aggattctca ggcaggcgaa
     4141 tccgctgctt caagccggag gacaccagga gacgaccttt agcgtcgaca atgatcgcct
     4201 cgacatccgg cagctgttcg acgagctcca ttccccgctc cggtcccatc acaaagatac
     4261 ctgtatcgag cccatcggcc cagacccctt cacgggcaat caccgtcaca ctctggcagc
     4321 tgcgcgctgg ctggagtacg cgagggtcga gaatatggtg atatcgaacc ccatcccgtt
     4381 cgaagaagcg ttcgtaatcc cccgccgtag aaatagcttc gtcctgcaac tcaacccaga
     4441 ccagcaccga ttgatcctct cgtggatgct gaatgccgac gggaaacatc ttgccattcg
     4501 gtaaatgccc aaatgttttg atatcccctg acagtgc
//
LOCUS       DATIPF010000404         2838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1229554, whole genome shotgun sequence.
ACCESSION   DATIPF010000404 DATIPF010000000
VERSION     DATIPF010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229554"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1908
                     /locus_tag="VLL06_10085"
     CDS             <1..1908
                     /locus_tag="VLL06_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HSS31355.1"
                     /translation="PAGPPLITNLVNVGILATKVGAGLGYHGKELERLTFAGLLHDIG
                     LFAVPQSLITKSGRLTQDERTLIEQHPELGYQAIRKAGSQYGWLAQVVSQAHERWNGQ
                     GYPNKLKGRQIGEFAQIIGVVDIFDALVSPRGYRRRFFPHEAVRELMVAERTAFPREI
                     VKALVEQLSAYPLGTSVRLTTGEVGSVMCINTRYPLRPVVWIEGDATCGQEGRQLDLS
                     LTPLVSIIETLEPPDVARVSFRIDETSAKTPDVVPSASDQFTSLLEGLDALASAIQGA
                     VESRTPMPESGTASLSREHRWRDRPPAQDLADASFEKEVIGLFALEAHEWLAQIQTAV
                     KQLAEGTNGTLRPKLYGILLQGITNLAKSASTVQLRTIEQMASNLLPILHDVGQQEPR
                     AMAAALQSLQEGLDGISLAVRRLAGDQGDEGTGQDDMPLEPAHEFVEALPPIAPEPLA
                     TTQEPSPLTMSASGSLIHALRDLQQARARSMQPTRDVLEAVIQRAEREGDHLDIAGVG
                     QILTELDRLDEEFLQEVRRRVPIISSALANLRTHGSPDFVTASQLDPILEQVEALYDL
                     ASHVQATMITMFLQGLRSFLMVAAYRKTSTLAQRLGTVETRIHALIPMAEQWGNIGRV
                     ERSAINDILPL"
     gene            2599..>2838
                     /locus_tag="VLL06_10090"
     CDS             2599..>2838
                     /locus_tag="VLL06_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31356.1"
                     /translation="MSMSLGLEGWYDATLLLPFLVEDEKSAASVVGERKMPKLVTVLR
                     PEKIEEQARFYVRSHILLPSGTIGLVCLIGGVGALG"
BASE COUNT          602 a          744 c          816 g          676 t
ORIGIN      
        1 cccgcgggcc ctccgctgat tacgaatctt gtgaatgtcg gaattctggc gacgaaggtc
       61 ggcgctgggt tgggatatca tgggaaagag ctggaacggc tgaccttcgc ggggctcctg
      121 cacgacatcg ggctttttgc cgtgccgcag tcgctgatca cgaagtcggg tcggttgacc
      181 caagatgaac gcaccctcat tgaacaacac ccagaactcg gctatcaagc gattcgaaag
      241 gctggttcgc aatacggctg gctggcacaa gtggtgagcc aggcgcatga acggtggaat
      301 ggacaggggt atccgaacaa gttgaagggc cgccaaatcg gtgagtttgc ccaaattatt
      361 ggcgtggtgg atatcttcga tgctctggtg agcccgcgcg ggtatcgtcg ccgattcttt
      421 ccgcatgaag cggtacggga attgatggtg gcggaacgga cggcgttccc ccgcgagatt
      481 gtcaaagcct tggttgaaca gctgtcggca tatccgctcg gtacttcggt gagattgacc
      541 accggagaag tcgggtctgt catgtgcatt aatacgcgct atccgctccg tccggtcgtg
      601 tggattgaag gcgatgcaac ctgtggccaa gaggggcggc aactcgatct gagcctcacc
      661 ccgcttgtat caatcatcga aacactggag cctcctgacg tcgcacgcgt ctcgtttcga
      721 atcgatgaga cgtctgccaa gacacctgac gttgtcccct cggcttccga tcaatttacg
      781 tcgctcttag aaggtttgga tgctctcgcc agtgcgattc aaggtgccgt cgaatccagg
      841 acccctatgc cggaaagcgg gactgcgtca ctgtcgagag aacatcgatg gagagatcgg
      901 cctcctgcac aggatcttgc ggatgccagc ttcgaaaagg aagtgatcgg gttatttgct
      961 ctcgaagcgc acgaatggct ggcgcaaatc cagacggcgg tgaaacagct tgctgaaggg
     1021 accaatggaa cgttgcgccc gaagctctat gggattctct tacagggtat tacgaatctc
     1081 gccaagtctg cgtcgacggt acagcttcgg actatcgagc agatggcgtc gaatctgctt
     1141 ccgatactcc atgatgtggg tcaacaagaa ccacgtgcga tggcagcagc gctccagtca
     1201 cttcaagaag ggttggatgg gatcagcctg gctgtgcgtc gcttagccgg tgatcaaggg
     1261 gatgaaggta ctggacagga tgacatgcct ctggaaccgg ctcatgaatt cgtggaggcc
     1321 cttccgccca ttgcgccgga accgcttgcg actacccagg aaccgtctcc gctgaccatg
     1381 agcgcaagcg gctccctcat tcatgctctt cgagaccttc agcaggcccg tgcccggtcg
     1441 atgcagccga cccgagatgt attggaggcg gtgatccagc gagctgagcg ggaaggcgat
     1501 catcttgata tcgccggtgt cgggcaaatt ttaactgaac ttgaccgcct tgacgaagaa
     1561 ttccttcaag aggtccgtcg gcgggtgccg atcatcagtt ccgctcttgc gaatttacga
     1621 acccatgggt cgccggattt tgtcacggca tctcagctcg atccgattct tgaacaggtc
     1681 gaggccctct atgatctggc aagtcacgta caggcgacga tgatcacgat gtttttacag
     1741 gggctgcgat cctttctcat ggttgcggct taccgcaaaa cgtcgacatt ggcgcaaagg
     1801 ctgggaaccg tcgagacgag aattcatgca ttgattccga tggcggaaca gtggggcaat
     1861 atcgggcgcg tcgaacggtc ggccattaat gacatcctgc cgctgtagtc cacagtacac
     1921 atgaacctct ctcgtatcct ttccgatttg cggttgcgtc gaaccagttg gttcgcagcc
     1981 tcgtgatgta gttcttcaac atgtatgtac aggacaggtc agtgcggcag cgttgtgcgc
     2041 cgtccctaaa cgcgactgcg tgatgtagtg caccacagca agatttttgg tcttcccggc
     2101 cctatcagtc tgcacacttc tgtacgccta atcgtaaaga atgatcttgc tcgaatagaa
     2161 ttcatacgtt cgctccctat gggtttccgt tgaaagaagt gcatcttggc gttattgcac
     2221 ggattgtgct gcctactgat taggcaaggt gtgaggtgct gtggtacggc atggtattgg
     2281 ttgttcatcc acatggttat ccacaaacca tgcacaactc ttgtggacac taatcgtgag
     2341 gctggcgtta tcctgcctcg ctgtcgcccg ttccctcgcg caacttcacg aggatgatga
     2401 tgaaagacct atctcagaga gacgtttcgg tgactctgtc tcccttcata ccgtggagta
     2461 agccgatgag tgcccttgat atgaatccca ggctagtcat ctgcctttac tcatgtctca
     2521 ttcacaattt cgcatgaagt caggggaatt gcgcgtcctg ctcgatcaga ccaggcacag
     2581 acgttcaatg tcctattctt gtcaatgtca ctagggctgg aaggatggta tgatgccacc
     2641 ctcttgctgc cttttcttgt cgaggacgaa aagtcggcag cgtctgtggt gggagaacgt
     2701 aagatgccaa aacttgtcac ggtattgcgg ccggaaaaaa ttgaggagca agctcgcttt
     2761 tatgtccgtt cccatatcct cctcccgtcc ggcaccatcg gactggtgtg cctgatcggt
     2821 ggggtggggg cacttggg
//
LOCUS       DATIPF010000405         4679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_520632, whole genome shotgun sequence.
ACCESSION   DATIPF010000405 DATIPF010000000
VERSION     DATIPF010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520632"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(663..1556)
                     /locus_tag="VLL06_10095"
     CDS             complement(663..1556)
                     /locus_tag="VLL06_10095"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HSS31357.1"
                     /translation="MFTDLRHIRLGTSSWAYEGWRGLIYQRAYTKSRFSQDALAEYAG
                     YEMDGILLFRTVGIDHSFYRPASAAQLAHYAKQVPEDFRFCSKVWEEITIPAFANLPR
                     YGAKAGKPNPRFLDASVFRDLVWAPAHESLGSKLGPFIFEFQRWGMEPAAFLDALDRF
                     LGALPPGPQYATEVRNPAILGSRYLNILRTHQVSHVYNHWTAMPALSEQHRLLEQTFT
                     APHVVIRLLTPLGLAHEKAVERYAPYDRIIAAQPRMRQDTTALVQQSVSQGKSAFVLV
                     NNRAEGCSPLTIQSLVAGLTS"
     gene            1640..3889
                     /gene="malQ"
                     /locus_tag="VLL06_10100"
     CDS             1640..3889
                     /gene="malQ"
                     /locus_tag="VLL06_10100"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HSS31358.1"
                     /translation="MQDQTEQALLAQLAEHAGIAKDYYDIAGTLHHTSDDTRRAILTA
                     MGYSIDSAASLTQALREWDEAPWQRPCDPVRILRDGETELSVSCYLALEDGKEGSVAV
                     EWQIRDEANAVVEEGQAGPGLSATEIRFLNGKRHVRVEIRAPCHLSLGYYSMTVRSEG
                     LVGGIVGTMRVIVAPRQCYAPPWLEASQRMWGLALQLYSLSSDRNWGCGDFTDLGRIV
                     EWAGKELAARVIGLNPLHALRNTSPYHISPYAPYSRLYLNELYIDLERLPEFYGSEEA
                     QQQFRAPEFQAKLQALRASRQVDYDAVQTAKRTMLELAYRRFLTESYSGEEPNLQPKT
                     SRARLLEQFIQSEGTSLELYATFQALEEERRLIQPNLATWHDWPNQFLTPGQPVREYA
                     KRHRTRIRFFEYIQWVASEQLNEIRQTAEQLGMPIGLYQDLALGAERNGAEAWIYQSV
                     LAFKADCGAPPDAFAPKGQNWGLPPMNPHALRASGYELMIQLLRNNFRLGGAIRLDHV
                     MALFRLFWIPLGRPASEGTYVHYPFEDLLAIVALESVRSKTLVIGEDLGTVPDWVREQ
                     LAKARVLSYRVFYFERGADGAMKSPGDYPSQSLAVATTHDLPTLTGFWTGEDLHVRAK
                     LGAFIDDAAQRQAWEERQRDKASVLHALQREHLLPQGVTEDLATAPAMTTDLCQAIHI
                     YLARTPSCIVLANVEDGLGELSQTNLPGTIDSHPNWTRKYAVQVDEMACDERLRQLGA
                     VLRSTRPLG"
     gene            4146..>4679
                     /locus_tag="VLL06_10105"
     CDS             4146..>4679
                     /locus_tag="VLL06_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31359.1"
                     /translation="MLKQNRIVLAIAGAVFLAIVVLEMAIPANVVGAYGFVLPILLIA
                     TVRSRGLMLMTVVACILATYLGLMQPTKPGRFQSAVINRSVVAGVLLIVAYIGMTWEE
                     RKAREEAARAALARETENLLKANTQLVSAKDQLNRSERLAAVGQLVASVAHEVGTPLH
                     SIAWHVQALAEEPGVTPE"
BASE COUNT         1043 a         1282 c         1331 g         1023 t
ORIGIN      
        1 tgcgcctttc gtagcttgat gaacgctaag gcctgttggc gatttgagaa gcagcggtca
       61 ctgaaattgc cggacactgg ccgctttcag ggggagcgat tctcttcccc tgggtatccg
      121 cagcggaaca gcagctagaa ggatgcccac taccggcttc agtcgtcgca cgaacactct
      181 cgatccggag ctgcgtggcc ttattctgta atgcagccag caagtcgtcg gaaacagttg
      241 cgctagccat cggagctcct ttcgggattt ggatgctaag aatgctgccg gactcttctc
      301 agtcttctct ctatcggcaa taacgtggtg ggtcttgagg ctaacaaaat gaaaaggggg
      361 agtcaatcaa cctcaccacg cctgtcaatc tagtttagac atactcacct ctcaccgatg
      421 cagacatgtc cgggttactc catccgtagt ctcccttccg tgacgacagt cagctcagat
      481 gcgccgatgt caccactggt gctcgaagaa gcgctgtctc caaagcggaa ccggaaagtc
      541 cgtacggcat cgacttcaag aacacctcaa gatcgtactg cacgaagagc gattgagtct
      601 ttcgaggaaa cacctcctcc cactgtgacc atgcacacga ttggagatcg aagagaatga
      661 ccctaactcg taagtccagc taccaaggac tggatcgtta gcggtgagca tccttcggct
      721 cgattattca caagcacgaa cgcactcttt ccctggctca cactctgttg aactaacgcc
      781 gtggtgtcct gtctcatgcg aggctgtgca gcgatgatcc ggtcgtaggg tgcgtatcgt
      841 tcgactgctt tctcatgggc gagtcccagc ggcgtcagga gccggatcac cacatgagga
      901 gcagtaaacg tctgctcgag caggcgatgt tgctcggaaa gtgctggcat ggccgtccaa
      961 tggttgtata cgtgggagac ctggtgtgtg cgcaggatgt tcagataccg tgatccaaga
     1021 atcgcggggt tgcggacttc agttgcatac tgtggtcctg gtggcaatgc gccgagaaat
     1081 cggtccaacg cgtcaagaaa ggcggcaggc tccatgcccc agcgttgaaa ttcaaaaatg
     1141 aatggaccaa gtttcgatcc caagctctcg tgtgccggag cccacaccaa gtctcgaaac
     1201 acactggcat caagaaatcg tggattgggt ttcccagctt tggctccgta gcggggaaga
     1261 tttgcaaatg ccgggatagt aatctcttcc cagaccttag aacagaagcg gaaatcttct
     1321 gggacttgtt tcgcatagtg cgcgagttgc gccgcactgg ccggacgata gaaggagtgg
     1381 tcaatgccga cggtacgaaa gagtaggatt ccatccatct cgtagccggc atactctgcc
     1441 agagcgtctt ggctgaatcg gcttttcgta taggcccgtt gatagatcaa cccccgccag
     1501 ccttcatagg cccaggagct cgtcccgagt cgaatgtggc gtaggtcggt aaacacgcga
     1561 aagcaattat acagaattta tctttaagaa caatgggata gcttgacatg agtggcagtc
     1621 ttcgcgcaga ctgccactca tgcaggatca gacggagcag gcccttcttg cccaactcgc
     1681 tgaacatgcc ggcatcgcga aagactatta cgatatcgca ggcacgcttc accatacctc
     1741 tgatgacacc cgacgcgcca tcctcactgc tatgggctat tcaatcgatt cggctgcatc
     1801 actgacacag gcattgcgag aatgggatga ggcaccgtgg caacgccctt gcgacccggt
     1861 gcgaatcctg cgtgacggcg aaacagaact ttctgtgtcc tgttatctgg cgctcgagga
     1921 cgggaaagaa gggtccgtcg ccgtggagtg gcaaatccgc gatgaagcga atgccgtggt
     1981 ggaggaaggg caggcaggtc ctggactttc cgctacagaa atccgctttc tcaatggcaa
     2041 acgacatgtg cgagtcgaaa tacgtgcccc atgtcatctc tcgctcgggt actacagtat
     2101 gacagtacga tcggaaggtc tggttggggg gatcgtgggg acgatgcgtg tcatcgtagc
     2161 gccgcgtcag tgctatgccc caccatggct cgaggcgagc caacggatgt ggggactggc
     2221 cttgcagctc tattcgttgt cctccgatcg taattggggg tgcggcgact tcaccgatct
     2281 tggccggatc gtggagtggg ccggaaagga actcgcagcc agagtcatcg gactcaatcc
     2341 gctgcacgca ctccggaaca cctcgccgta tcacatcagt ccctatgccc catacagccg
     2401 actgtatctc aatgaactct acatcgatct cgagcggctg ccggagtttt atgggtcgga
     2461 ggaagcacag caacaattcc gtgcgcccga atttcaagcc aagcttcagg cactgcgagc
     2521 gagccggcaa gtggactacg atgctgttca aaccgccaaa cgaacgatgc tcgaattggc
     2581 gtaccgcaga ttcctgacag agtcctacag cggagaagag ccgaatctcc aaccgaagac
     2641 atcgcgcgcc cggctcttag aacagtttat ccagtccgag ggcacatcgc ttgaactgta
     2701 tgccaccttc caggcgttgg aggaagaacg ccggctgatt caacccaact tggccacctg
     2761 gcatgattgg cccaaccagt ttctgacgcc tggccaaccg gttcgtgaat atgcaaaacg
     2821 acatcgaacg cgcattcgct tctttgaata cattcagtgg gtggcgagtg aacagctgaa
     2881 cgaaatccga cagacggccg agcagttggg gatgccgatc gggttgtatc aggatctggc
     2941 tcttggggct gagcggaatg gggccgaggc ctggatctat caatcggtgc tggcgttcaa
     3001 agccgactgc ggcgctccac cggatgcatt tgccccaaag ggacagaact ggggcttgcc
     3061 tccgatgaac cctcacgcgc tacgcgccag cggctatgag ctgatgattc aactgttgcg
     3121 taataatttc cgattgggcg gagccattcg attggaccac gtcatggcac tcttccgtct
     3181 cttctggatt ccactgggta gacctgcctc cgagggaacg tatgtgcact acccctttga
     3241 agacttgttg gccatcgtgg cattggaaag tgttcgatcc aagaccctcg tcatcggaga
     3301 agacctgggc acggtgccgg attgggtcag agagcagttg gccaaggcga gagtgctgtc
     3361 atatcgggta ttctacttcg agcgcggtgc agatggcgcc atgaagtcgc ctggcgacta
     3421 tccctctcaa tcattggctg tggccacaac acatgacttg ccaacactca ccggcttttg
     3481 gaccggcgaa gatttgcacg tgcgggcaaa gttgggcgcc tttatagatg atgcggcgca
     3541 gcgacaagcc tgggaagaac gccagcgtga caaggcaagc gtcctccacg ctcttcaacg
     3601 ggagcacctc ctgccacagg gcgtaacaga agatctggcc accgcgcccg ccatgacgac
     3661 tgatctctgc caagccattc atatctatct ggctcgcacg ccatcctgta tcgtactggc
     3721 gaacgttgaa gacggcctgg gcgaactttc acaaactaat cttcccggga cgatcgacag
     3781 tcatccaaac tggacccgta agtatgcagt tcaagtggat gagatggcat gtgatgaacg
     3841 cctgcggcag cttggcgcag tattgcgttc aacccgtccg ctagggtaag accctcttat
     3901 gccggatgct gaaaaagtcc gccagcttcg ttctcggctc atcgaaatcc tcaacgtacc
     3961 cctgagggta cgcctccggt atcgattcgc ctgcggcttt gctggacaga ctttttgagc
     4021 atccggtagt gcgttgttcc cctagatata ctggtctctg aatccctact ggacaacata
     4081 attcttctac agcctgtttg agagtggacc atggttcatc agcagtctag gcatcgccta
     4141 ttcccatgtt gaaacaaaac cgcattgtgc tggcgatcgc aggggccgtg ttcttggcga
     4201 tcgtcgtgct cgaaatggcc atcccggcga atgtggtcgg cgcgtacggt tttgtgttgc
     4261 cgattctgtt gattgcaacg gtacgcagtc gcggccttat gctgatgacg gtggtggcct
     4321 gtatcctggc gacctatttg gggctgatgc aaccgaccaa gcctggacgg tttcagtcag
     4381 cggtgatcaa tcgaagtgtg gttgccggag tgctcctgat cgtggcctat atcggcatga
     4441 cctgggaaga gcgcaaggca agagaggagg cagcgcgtgc agcgctcgcc cgggagacgg
     4501 aaaatctctt gaaggccaat acccagctgg tctctgcgaa agaccagctg aatcggtcgg
     4561 aacgccttgc cgctgtaggg caattggtgg cttccgtcgc gcatgaagtg ggaacgccgc
     4621 tgcactcaat tgcctggcac gtgcaagcgc tggctgaaga gccgggcgtt acacctgag
//
LOCUS       DATIPF010000406         6791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1140388, whole genome shotgun sequence.
ACCESSION   DATIPF010000406 DATIPF010000000
VERSION     DATIPF010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140388"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(421..762)
                     /gene="smbP"
                     /locus_tag="VLL06_10110"
     CDS             complement(421..762)
                     /gene="smbP"
                     /locus_tag="VLL06_10110"
                     /inference="COORDINATES: protein motif:HMM:NF028095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HSS31360.1"
                     /translation="MISKVYRGALILAALGAFVSVPMVNAQPSPMGNVREAIDHARQA
                     VAHGKEGHADELVKHAETSLQFAKMGGRGSHLTEGINHLKEAIEHGKAGHADVGTEHV
                     ETALQHLSEVK"
     gene            complement(1376..1810)
                     /locus_tag="VLL06_10115"
     CDS             complement(1376..1810)
                     /locus_tag="VLL06_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31361.1"
                     /translation="MPVPAQPVALHPANVEPFVGVAVKITVVPMMKFAEQLLVHWVMP
                     PGELFTVPLPVPMLVTVRAAVLGGAVKVADTVCGAFITTRQEPIPVQPPVQLANVEPD
                     VPLAVRVTELSLAKSALQVGPQLIPAGSLVTMPSPLLVTDSN"
     gene            1815..3044
                     /locus_tag="VLL06_10120"
     CDS             1815..3044
                     /locus_tag="VLL06_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31362.1"
                     /translation="MVLVNASQTVTATFTKPLPVMDLTIYGSDQGLSGRVIDASPDDN
                     QNIWAVTPDALYVLQPGQTTFKRFTAADGLHIQSFTDPNGQPAVTTITAMAGGHANEV
                     FVGYRGFEGEIPPPAPPPCCVPNADFSDPRWALGQADKVTLNPDGTIQIRQYLFRCDI
                     SVNCWEERSARRMTFAHQGVATGHLFIGFDHGVAHVFNDIFGDHIHVITQYHFPDNPI
                     PVQKQGEQYGLFVLPTGELLSGGAYGVGLQQWNPDPRAWVKEPFLWAFTTYGPAESYN
                     TGPHTLDVPAGYREDQRGVTMTSDGTAWLASLTQGLASYNNRDAGGNFDRIQTYTTVP
                     GLPSSGLMDIAADPDGSLWIVDRNGRLLRFTPSTLAVQVWPGISGALRVVMDTSVTPR
                     AVYVSMGTNGLGVIRAK"
     gene            complement(3100..3300)
                     /locus_tag="VLL06_10125"
     CDS             complement(3100..3300)
                     /locus_tag="VLL06_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31363.1"
                     /translation="MRLEVQEYMRASARLFGLAYKTDALTLAERAAIVAFAQELEKKF
                     LPSREERDVPLSATLSNARLMD"
     gene            complement(3487..4179)
                     /locus_tag="VLL06_10130"
     CDS             complement(3487..4179)
                     /locus_tag="VLL06_10130"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HSS31364.1"
                     /translation="MYSIFIKSDILLVMTSENKFPSSVFRLFRTHGVAEAIRARCWYH
                     LELCFYACDEKNILTLPRHQFLRRDCFDDLHYYARDERGQMSPDTFRRESTKRQALGY
                     HLYTIVQDGRMVFYAWLVDRQARSEDPMLGQVFFPPTDASIIFDCFTRPADRGKGIYY
                     RALCQMLHDARDIAQARQVCMGVFADNPASCRVIEKVGFRYIGSMIKHRRLFKTHRYP
                     VAVNHKFKTALL"
     gene            4492..5043
                     /locus_tag="VLL06_10135"
     CDS             4492..5043
                     /locus_tag="VLL06_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31365.1"
                     /translation="MKTRTIVWIATCGLLSLPSLAFSQDQPVTGDKSVVFPPSFKCST
                     NSPCRNLTGEILRIEESYWIQGPEGEETHVKVTRDTKMAELPKVGDKIAAQLTSKGEA
                     NAIVKLAEIPKPKDLPAPPHAQKDFREGQSSQPAGSGQAKQGQDHIQQSDAEVGGKMQ
                     SIDPLSGGGNIDPSSKMDERPTR"
     gene            5344..>6791
                     /locus_tag="VLL06_10140"
     CDS             5344..>6791
                     /locus_tag="VLL06_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31366.1"
                     /translation="MTFYPLFIVTIFASLFGIGSVEAAGLDALQQVVGQRASGLITGL
                     SGAPVLGSVGGLPRDAVLGQTVTIADGVTTGPNDRVEILWDRRAVILVQPDSKVLIQE
                     SKAGQTDFSLSGGSIRVALAYGGASTDMVTVQTPSSRVYTRGGIFEVDVRPPMPSFIA
                     RVVSALSPPISHAPVETVRVHEGQSGIEPGSAPTPGGSHMLEAGVLARIAMGIVEQVE
                     ALPKTSAKGLGLAETDRQQATPALLTQRLVNIHVTHAVEVARVMSTPSTTIDETAATP
                     GSTLKGTIVATSLGVPSIPNAQAGTTKGVAPGSPPAASVTSPVPTRPPMQVPPITRPP
                     NGLSRHALKEGLGDEDKHSHQSKGHDRNQDSSAIAITSPLPTLLGVPPSTNTPLTSTQ
                     TVGPASSTVLKNVLDDGKSGGTHHSGGTEQVSSNPVMTSPIPTLPAIEIPTTTTMAPS
                     QAGGFNSHQLLKDAFDLDKGGGKEKHQGGDRH"
BASE COUNT         1571 a         1923 c         1839 g         1458 t
ORIGIN      
        1 ggtatgacga gatgaacaga agaactggtg tccccaccgg gaatgttagc cttttaaccc
       61 cacatctcaa ctctctgata atcccatttt cagaggagac tcaggcccgc ggagcttagg
      121 atacaatgtt gtgtttttcg gcattcccac ggtggcactg gacgagggtc ttcccacagt
      181 ccacgcttcg tctgttgcac ctagatttct agtttctcta gcaccctatt ccccggcgca
      241 tacttccaat accaccagca ccagccgtct ttcactaact cgtaattgac gtgggtgccg
      301 tccggcctgg cacccttgca ggaggagtca aacaaaaatg gcggctccat ttctggagcc
      361 gccgtctgtg acgatgtggc aggttctacc cgcgcctgca tgcgctcgtc agaatgtccg
      421 tcacttgacc tcggacaaat gctgtaaggc ggtttcgaca tgctcggtgc ccacgtcggc
      481 gtgaccggct ttgccgtgtt ctatggcttc tttaagatga ttaatccctt cggtcaaatg
      541 cgaacctctc cctcccatct tcgcaaattg cagtgacgtt tccgcatgtt tcacaagctc
      601 atcggcatgc ccctctttgc catgggccac cgcttgtctc gcgtgatcga tggcctccct
      661 gacattaccc atgggggatg gctgggcatt caccatgggc acactcacga aggctccaag
      721 cgcagccaga ataagggcac cacggtagac tttgctgatc atcttttctc cctccatggt
      781 tagaattgac tgccgaacaa gactatagaa aatcgtagat tcattttaat acagcgtttc
      841 agaaatccca ctccaggagc ggtctacaca gtcttctggt gtcctcaacg ggatccgact
      901 gaccacggag cgcccactaa gcttcagcat ggaccagctc gtggtggccg catgagccta
      961 tgcctcgccc ctccaaaccg cggcgagcat accatacgga aagtgaatat cgccagccat
     1021 cgcacgctct accgatgatg agcagccagc agaaatcttt cttcaactga agttcaccga
     1081 cgcctttgcg cagaacttcg atggtgtcgc cgtctagaac ggagacaatg gggagcgtgt
     1141 gacggttcct catgcaagca agggttcctc ccagtatagg agatccccta gccctgctaa
     1201 gaaatttaat taagagtggc gtggggcaag tccaaattct gcaggggcag cagatgtgtt
     1261 ggcgaagttc gacgacatgg gccgtgtttc tgttgttgtg tgcctgtgct ggctccgacg
     1321 attcatcgac actcgtgccg ccaggatcaa aaccgccacc cgctccatct ccatctcagt
     1381 tgctgtctgt caccaaaagc ggcgacggca ttgttaccag cgaaccggcc ggtatcagtt
     1441 gcggtcccac ctgcagtgcc gatttcgcga gcgacagctc cgtcaccctg actgccaatg
     1501 gtacgtccgg ctcaacgttc gccagctgga ccggtggctg cactggaatc ggttcctgcc
     1561 tagtcgtgat gaatgctcca caaaccgtgt ccgcgacttt cacggcgcct ccgagtacgg
     1621 cggccctcac cgtgaccaac atcggaaccg gtaacggcac agtaaatagc tcaccaggcg
     1681 gcattaccca gtgcaccaac agctgttcgg cgaatttcat catcggaaca accgtgattt
     1741 tgaccgcgac gccgacgaac ggctcgacat ttgcgggatg gagcgcaacc ggctgtgcag
     1801 ggaccggcac gtgtatggtt ctcgtcaatg cctcacagac agttacggcg acctttacta
     1861 aacccctgcc ggtgatggac cttacgatct atggctctga tcaagggttg agtggcaggg
     1921 tgatcgacgc gagcccggac gataatcaaa acatctgggc tgtgacgccg gatgccctgt
     1981 acgttcttca gccgggccag acaacgttca agcggtttac cgcggccgat gggctccata
     2041 ttcagtcgtt cacggatccc aacgggcaac cagccgtcac aactattacg gcgatggcgg
     2101 gtgggcacgc caatgaagtt ttcgtagggt atcgaggatt tgagggcgag attccgccac
     2161 cggcgcctcc gccgtgctgc gtgcccaacg cggattttag cgacccgcgc tgggccctcg
     2221 gccaagccga caaagtgacg ctgaatcccg atggcacgat tcagatacgt cagtatctat
     2281 tccgctgcga catcagtgtt aactgttggg aggaacggtc cgcacgacgc atgacgtttg
     2341 cgcatcaagg cgtggccacg gggcatcttt ttatcggctt tgatcatgga gtcgcccacg
     2401 tcttcaatga catctttggc gatcacattc atgtcatcac ccagtatcac tttcccgata
     2461 acccaatacc ggttcagaaa cagggtgaac agtacggatt gttcgtgttg ccaacgggtg
     2521 aattgctcag tgggggggca tatggtgtcg gattgcagca atggaacccg gacccacgag
     2581 cctgggtgaa ggagccgttc ctctgggcat ttacaacgta tggaccagcc gagtcctata
     2641 acacagggcc ccacacattg gatgtaccgg cgggatatcg agaagatcaa cgcggtgtga
     2701 ccatgacctc cgacggcacc gcatggttgg cgagtttgac gcaggggctg gccagttaca
     2761 acaacaggga cgcgggcgga aattttgatc gcattcaaac ctataccact gtgccgggcc
     2821 ttccctcgtc ggggctcatg gatatcgccg ccgacccgga tggatcgttg tggatagtgg
     2881 accgcaacgg gcggctactg agatttactc cctcaaccct ggctgtccaa gtgtggcccg
     2941 gcatatccgg tgcgctccgc gttgtgatgg atacgagcgt cactccgaga gctgtctatg
     3001 tatctatggg caccaatggt ctgggggtga tcagagccaa gtagatcagt cgtgctacga
     3061 aggcaagcct aagtggtggt atcccctatg tctttcgggt taatccatca ggcgtgcatt
     3121 ggagagggtt gccgacaacg gcacatcgcg ctcctcacga gagggaagga atttcttttc
     3181 gagctcttgg gcaaaggcga caatagccgc acgttccgcc agagtcagcg catccgtttt
     3241 atacgccaat ccaaagagcc tggcgcttgc gcgcatatat tcctgcactt ctaaccgcat
     3301 gagtcggact gtagcatgtg tgatcaaacc aggcggactg ggaagaaccc cagcggaaga
     3361 tgatttcagc gggaatcatt ccggacagcc agtgttctcg tcacatttct tcggcaaata
     3421 ctcaccacaa ttgccggatg atccacctgg gagggggcag actcatcaat cacattgctt
     3481 gttggattac aataacgccg tcttgaattt gtgattcacg gcgacaggat agcggtgagt
     3541 cttgaacaaa cgacggtgct taatcatgct tccgatatag cgaaatccca ccttttcaat
     3601 gacacgacaa gaagcggggt tatcggcaaa gacacccata cacacttgcc gcgcctgagc
     3661 gatatctcgc gcatcgtgca gcatctggca cagcgctcga tagtagatgc ccttcccccg
     3721 gtcggctgga cgcgtaaaac aatcgaatat gatggaggca tctgtcggtg ggaagaacac
     3781 ttgtccaagc attgggtctt cactccgtgc ctgtcgatcg accagccacg cataaaagac
     3841 catacgacca tcttgaacga tcgtatagag gtgatagccc aatgcctgcc gttttgtgct
     3901 ctcgcgtcga aacgtgtcag gcgacatctg cccacgctca tctctcgcat agtagtgtaa
     3961 atcatcaaaa caatcccggc gcagaaactg gtgtcgggga agagtcagga tgttcttctc
     4021 atcgcaggca tagaagcaga gttccagatg ataccagcat cgagcgcgaa tggcctcggc
     4081 aactccgtga gttctgaaga gacggaatac actggatggg aatttgtttt cagaggtcat
     4141 gacgaggaga atgtccgact taatgaagat ggaatacacc tacctttacc agattacgca
     4201 gaacgttctg cctagtgatt cccccagggc agcgactgat gtacatcgga ataacacccc
     4261 acgacgtgac ccaggtgagc cgccaggggt agcgccaaat ccttctgcag cgccttgagc
     4321 aacaccattg cctagattga gaccggctca gcgatcagag caatgcgccc catcctaggg
     4381 gagctgacag taccgtacaa gtcagcgcgg tcttagaatt cgactgagcc ctgagcgctg
     4441 agttttatta tttttaattc tcccaccatc ggaatatgaa aggagaatgc tatgaaaaca
     4501 cgcacgatag tttggatcgc tacttgtggc ttgctgagct tgccctcgct ggccttcagt
     4561 caagatcagc cagtgacggg ggataagtcg gtagtatttc caccgagttt taaatgttca
     4621 acgaattctc cttgccgcaa tttaaccgga gaaattttgc gaattgaaga gtcctattgg
     4681 attcaaggtc ctgaaggaga ggaaacgcac gtgaaagtta cgcgagacac caagatggcg
     4741 gaactaccga aagtcggcga taagatcgcg gcacaactca cctcgaaggg agaggcgaat
     4801 gcgatcgtaa aacttgctga aatccccaaa ccgaaggact tacctgctcc tccccatgcg
     4861 cagaaagact tccgggaggg ccaatctagt caaccagcag gaagcggtca ggccaagcaa
     4921 gggcaagatc atattcaaca gtcggatgca gaagtcggag gaaaaatgca aagtatagat
     4981 ccgctgtcag gcggagggaa catagatccg agttcaaaaa tggatgaacg cccgactcgt
     5041 tagggcacga ggtacgccgt tctcgtgtga acggcgtacc gatttcattc aggaaccaga
     5101 gattccttat ggtttggcgt tatgcgtcca aactaaaaca tagaaccgtc catgtctatg
     5161 ggggaaacat tgaatcacca gccagttcca ccgcaccttt accgggatga tttaggggta
     5221 tttttcatct cattctgggg ttgttccgag ttcgtaccgg tcctcagaca agataagtaa
     5281 gagagccaca agcatgggat gcgcgatgtg tattctctgt accctattta cttttggtag
     5341 cttatgactt tttatcctct cttcatcgtc accatcttcg cctcgctatt cggaatcggc
     5401 tcagtggagg ccgctgggtt agacgcactg cagcaggttg taggacagcg agcctctgga
     5461 ttgatcacgg gtctgagcgg ggctccggtg ctgggctcgg tcggtggcct cccccgcgat
     5521 gccgtgcttg gtcagaccgt gacgatcgcc gacggagtga ctacaggccc caacgaccgc
     5581 gtggagatcc tctgggatcg tcgagccgtg attctggtac aaccggactc gaaggtcctg
     5641 attcaggaat cgaaagccgg gcagaccgat ttctccctca gcggggggag tatacgtgtg
     5701 gcgctggcgt atggtggcgc atcaacggat atggtcaccg tgcagacgcc atcctcccga
     5761 gtgtacacac gtggaggaat ctttgaagtg gatgtccgtc cgcccatgcc gtccttcatc
     5821 gctcgcgtcg tctcagcctt gtcacctccc atcagccatg cgccggtcga aaccgtgcgg
     5881 gtacacgaag ggcagtctgg catcgaaccc gggagcgcac caacgcccgg agggtcccac
     5941 atgttggagg ccggcgtcct cgctcgcatc gccatgggaa tagtcgaaca ggtggaggcc
     6001 cttcccaaga cttccgccaa aggcctagga ttggcggaga cggaccggca acaagcgacc
     6061 ccggctctgc tgacccagcg cctcgtcaat atccatgtca cgcatgccgt ggaagtcgcc
     6121 cgtgtgatga gcacaccgag tacgacgatt gacgagacgg cggcgacacc tggttccacg
     6181 ctgaaaggca cgatcgtggc cacgagttta ggtgtgccct ccataccgaa cgcccaagca
     6241 ggcacgacaa agggggtcgc tcctggatcc ccgcccgctg ccagcgtcac gtcaccagtg
     6301 ccaactcggc ctcccatgca ggtcccgccg attacgaggc cgccaaatgg actcagccgc
     6361 cacgcgctca aggaggggct gggcgatgag gataaacaca gccatcaatc gaagggtcat
     6421 gaccgcaacc aggattctag cgccatcgcc atcacctcgc cacttcccac gctactaggc
     6481 gtaccgccct cgacgaatac ccccctgaca tctacccaaa cggtaggacc cgctagttcc
     6541 accgtgctaa agaatgttct ggacgatggt aagagcggag gcacccatca ctctggggga
     6601 acggagcagg tatccagcaa ccctgtcatg acctctccga ttccgacact acccgccatt
     6661 gagatcccca cgaccacgac catggctccc agccaggcgg gaggattcaa tagccaccaa
     6721 ttacttaaag atgcgttcga ccttgataaa ggcggtggca aggagaaaca tcagggcgga
     6781 gatcgacatc a
//
LOCUS       DATIPF010000407         2732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1229810, whole genome shotgun sequence.
ACCESSION   DATIPF010000407 DATIPF010000000
VERSION     DATIPF010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229810"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VLL06_10145"
     CDS             <1..495
                     /locus_tag="VLL06_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31367.1"
                     /translation="GHVLIDNGDAAIATCITFDRPREYTVLVRYPAFAQKQAQRLWTI
                     CAELQRWDWLSHIQFVSHEGPHDGEAPHSYDVVYHWVPYDSGDLPASLAERIEAISRI
                     LREGGHAFVIGPAQMGQYGVSHGLQVCWEEPVEQLPTFRMHRTILPKARLRAGLTLFY
                     IKKV"
     gene            832..1527
                     /locus_tag="VLL06_10150"
     CDS             832..1527
                     /locus_tag="VLL06_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31368.1"
                     /translation="MSDPTPEHIIGEESDDKIIVYWEREYSTAIAPERLKLDFSPHRP
                     LLSGMLLSEQRTVAGEGYKVIPDDCGPVRTVGQYGWLIRSPVDCKIRRTADGVKWQAP
                     PILPEERILGYKSFSGTYVDLILNSGYPKLCCGIRFYYPKQVGLIMKDVPNHFYHYPE
                     RTFLVWEGMKTQEYKRTPNTYDWLPDYDAFTANFLLQLTKPTTIKRGDPIGIVVPVLL
                     PKQFTLEELTPRG"
     gene            1805..2353
                     /gene="pyrR"
                     /locus_tag="VLL06_10155"
     CDS             1805..2353
                     /gene="pyrR"
                     /locus_tag="VLL06_10155"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HSS31369.1"
                     /translation="MPQDRPQERLVMDAGDIARALTRIAHEILERNKGVQALALVGIR
                     TGGVHLAHRLVRRIQEIEAASVPIGELDITLYRDDLSLRKEQPILRKTSVPFDISDRI
                     IVLVDDVLFTGRTIRAAMDGLIDLGRPAEIQLAVLVDRGHRQLPIKATYIGKNIPTSR
                     EEKIQVLLEEEGEDDRVVISKA"
     gene            2370..>2732
                     /locus_tag="VLL06_10160"
     CDS             2370..>2732
                     /locus_tag="VLL06_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HSS31370.1"
                     /translation="MSLKRKDLLSLAPLSVDEIRLILQTADSFKEVTGREIKKVPALR
                     GRTVVNLFFEPSTRTRTSFELAAKRLSADVINFSPSSSSVVKGETLLDTARNIEAMQA
                     DIIVLRHPSAGAAETLARG"
BASE COUNT          601 a          729 c          817 g          585 t
ORIGIN      
        1 gggcacgtgc tcatcgacaa tggcgatgcc gccattgcga cctgcatcac gtttgatcgg
       61 ccgagagagt ataccgtgct ggtccggtat ccggcctttg cgcaaaagca ggcacaacga
      121 ctctggacca tctgcgccga gttgcagcgg tgggattggc tctcgcacat tcaattcgtt
      181 tcgcacgaag ggccgcacga tggggaagcc cctcattcct atgacgtggt ctaccactgg
      241 gtgccctatg acagcggaga cctgcctgca agcctggccg aacgcatcga ggcgatcagc
      301 agaattcttc gggaaggagg ccatgcgttc gtcatcggtc cggcacagat gggtcagtat
      361 ggtgtgtcgc acggactcca ggtctgctgg gaagaaccag tcgagcagct tcccacgttt
      421 cggatgcatc gcactatcct accgaaagca aggctgagag ctggtctaac attgttctac
      481 atcaaaaagg tctagcagga tgctgaaaca gcccgccagc gtcgttctca gtcgtgcgaa
      541 ctcctcaatg gggacccggc cgcctcacca ctcggcggcg cgcacaaacg tggtgctcct
      601 tattcgtcgc accgtgcgcc ccagagggta cgcctccggt ttcaactcgc ttgcggcctt
      661 gcttgggtca aggcgcgtct cggcgcgccc gggttgggcg ggtgagaagg acggcctgtt
      721 tgagcatcct gcggaggcat tctctccttg ttccagacat acaatccatc aaagcgccac
      781 catgtcaaaa tggttttccc gtaacctgtc agatcgatta caggtggcga gatgagtgat
      841 ccgactccag aacacattat cggcgaggag tcagatgaca agatcatcgt ctattgggaa
      901 cgggagtatt cgactgctat cgcaccggag cggttgaagc tcgacttctc gccgcatagg
      961 ccattgttgt ctgggatgtt actgagcgag caacggacgg tggcgggcga gggctataaa
     1021 gtgatccccg acgattgtgg accagtgcga acagtgggcc agtacggctg gctcattcga
     1081 tccccggtgg actgtaagat tcgacgaacg gccgatgggg tcaagtggca agccccgccg
     1141 atcttgccgg aagagcggat ccttggctac aaatcgtttt caggcacgta tgtcgatttg
     1201 atcttaaaca gtgggtatcc caagctgtgc tgcggtattc ggttttacta ccccaaacag
     1261 gtgggattga taatgaagga cgtgccgaac cacttctatc actaccccga gcggacgttt
     1321 cttgtgtggg aggggatgaa gactcaggaa tataaacgca caccgaatac ctacgactgg
     1381 ctcccggact acgatgcctt caccgctaac tttctattgc aactcacgaa gccgacgacc
     1441 atcaaacgtg gtgatcccat cggcatcgtg gttccggtgc tccttccgaa acaattcaca
     1501 ttggaagaac tcacaccacg tgggtaacct ctccacgggg gtggttgtca tacgattgtc
     1561 cacgagtcga aaaatacaag atgcccgggt gggtgcggtg gtgtccctac aaggagtgta
     1621 ttgcggcttg acaggattcc tccggcctca gtaagatgcg gcaaccttta atgctcatcc
     1681 tgcgaggtga gcaaggagtg tccatgggcc tcaatcaggt ttgtgtctgt cgacacaccc
     1741 gaaccttctt gtcggatctc gatgagaggg tttttttatg cgtggaatca ggacgatgag
     1801 ttccatgcca caggatcgac cccaagaacg gctggtgatg gatgcgggag acatcgcccg
     1861 ggcgctcacc cgtatcgccc atgaaattct cgagcggaat aagggcgtcc aggcactggc
     1921 gctcgtcgga attcgaaccg gaggggtgca tctggcccat agactggtga ggcggatcca
     1981 agagattgaa gcggcatcgg tgccgatcgg cgagttggat attacgctgt accgggacga
     2041 tctctccttg cgaaaggaac aaccgattct tcgcaagaca tcggtcccgt ttgatatttc
     2101 agacaggatc attgtgttgg tcgatgacgt gctgtttacg ggtcgaacca ttcgggcggc
     2161 gatggatggg ttgattgatt tggggcgacc ggcagaaatt caactggccg tcctcgtcga
     2221 tcgaggccat cgtcagttgc cgatcaaggc cacgtatatc gggaaaaaca ttccgacgtc
     2281 ccgcgaagag aagatccaag tgctgttgga agaagaaggc gaagatgatc gggtggtgat
     2341 ctccaaggcc tgaggagatg gagggaacga tgagtctcaa acgcaaagac ctcttgagcc
     2401 tggcccctct gtcggtcgat gagatccggc tgatcttaca gaccgccgat tcattcaagg
     2461 aagtgaccgg tcgggaaatc aaaaaggttc cggcacttcg agggcgcacc gtggtcaatc
     2521 tcttctttga gcccagtacc aggacacgca cctcgttcga gctggcggcg aaacggctga
     2581 gcgcggacgt gatcaacttt tcaccgtcgt cgagcagtgt ggtcaagggg gaaaccttgc
     2641 tcgatacggc gcgcaatatc gaggcgatgc aggcggatat catcgtcttg cggcacccgt
     2701 ctgcgggcgc ggccgagacc ctggcccgcg gg
//
LOCUS       DATIPF010000408         8705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_784345, whole genome shotgun sequence.
ACCESSION   DATIPF010000408 DATIPF010000000
VERSION     DATIPF010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8705
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784345"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VLL06_10165"
     CDS             <1..741
                     /locus_tag="VLL06_10165"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HSS31371.1"
                     /translation="CMMESEHIPVHSSVLVVGLPLQLVIGSGEDKVNCGSTLLGWKER
                     AWLICEWPFHFGQAVPCETGTHCLVRAMVAGRLVAYQSEVCMVQMSPLPLLYLAFPRR
                     IEEIHLRKDARVPSNEPLLLMQGTHRDAMLALRNGSAPIGGLLQDVSLSGCRIILQRS
                     RTDVVLGATVNLEFELIGIGHVTHLAGVIKNIAERDGAISIGIEFRFNGKEAIEYRGW
                     GGSVQKAIEYSVMQRQTDFGLLTPPSDP"
     gene            997..3789
                     /locus_tag="VLL06_10170"
     CDS             997..3789
                     /locus_tag="VLL06_10170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSS31372.1"
                     /translation="MPQAIEVSTDMGALLNSARAVFREIPMPLAACLALWRRAAAGGS
                     GSDPLAPRSAMSHTYPSTSRPEIAAVLEGLPLGEWAMTMAAIDFLLNQVERIRPHAIL
                     EFGSGASTLALACMMRRLHGASKTPHIFSVEQSPRYVQQIESRLRLHGLQESVRFMTA
                     PLISQVIQNVGTDCYQLRDQDLRTFFGQSKPDLVVVDGPAADYGGRFGTIPLVRNFLS
                     DHAWIYMDDALRDSELSIADWWDRLGYVKDCGVLWLGKGLLTARLHSPELLAHEPAHV
                     FLRQLLEPSEPGHRHHIHELPPAASLVPAPFPCERIAASDLREEAQELSPPSLTGRPT
                     CLFLNTYYSGFLTHHYRSMSERATQSYEAQRRSLQQTCFGDSDFYSSGMMLAGWQAED
                     MIVNCAPLQEAWAREQGYRGHRQLLDIAIEQIRRRRPEVLYLQDLGLATKEFLACIRP
                     HVTLIVGQIASPVPAHAKLKGFDLLFSSFPHFVEEFRKQGMTAYYQPLAFEPRIAHIP
                     APSDRAHAVTFVGGLSAAHKERQQFIASLAKLVPLECWGYGVDALRASGIDQTRLHGE
                     VWGDEMFSTLRQSLITINHHTDVAQTYANNMRLFEATGCGALLVTDHKDNLDDLFRTG
                     EEVIAYRSLDECAALVRYYIAHPEEARRIADRGQARTFRDHTYARRMAHTAEIVSRHL
                     EERQGHQRLPDPDLAHVSYGKEPIQPDEITTALATSWQSPSIPLKQRALVQQELTALY
                     QGGPPVVYRILADALRPFVAPDCSVLEIGCASGYYYEVLEYLLNTRLSYVGVDFSPAM
                     VALAQRYYPRAIFHVGNGAALNFPDQSFLIAISSCVLLHVADYSQHIREAARVASQIV
                     VLHRTPICRAHPTNHYKKFAYGVETYELRLNESEILGLCRDQNLELVQRLEYDSHPER
                     DEFEATYVFRKVGRYG"
     gene            3782..4786
                     /locus_tag="VLL06_10175"
     CDS             3782..4786
                     /locus_tag="VLL06_10175"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSS31373.1"
                     /translation="MDRGFRSAKVLITGGMGFIGSNLAHRLVTMGAEITLVDSMIPEY
                     GGNLWNIQEIRNHVQVNISDVRDEHSMKYLLQGCDYLFNLAGQTSHVDSMEHPQPDLE
                     INARAQLSILEGCRRHNPNIKVVFASTRQIYGRPQYVPVDELHPIQPVDVNGINKMAG
                     EMYHLLYCRVYGIRTAVLRLTNTYGPRMRVKDARQTFLGVWIRRLIEGQPILVYGDGT
                     QIRDFNYVEDVIDALLLAASTDKANGEVFNLGAGDPISLEDTAKLLITIYGKGSYELI
                     PFPSERQIIDIGNYYGNYHKIQSQLGWQPKTFLRAGVEQTLTFYGRYCMHYWQDVHAA
                     "
     gene            4776..5942
                     /locus_tag="VLL06_10180"
     CDS             4776..5942
                     /locus_tag="VLL06_10180"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HSS31374.1"
                     /translation="MLRKPIPLADPGAQMRLCGESMRSAFDRVLTSGRYILGNEVEAF
                     EREWAAYLRARHCIGVGNGTDALALALLAAGVKAGEEVITVSHTAVATVAAIEQIGAV
                     PVFADIDPSSRCMDPAHLPGLISENTRAIVPVHMYGQPAPMGKIMAVAKEHGLKVIED
                     CAQAHGATLGGQYVGTFGDAAAFSFYPTKNLGALGDAGAIVTNDATLAASLRMLREYG
                     WQERYVSLTPGRNSRLDELQAAILRAKLPDLDRHNIRRRAIAAAYTQALRNTALVAPA
                     QIERTEHAMHLYVLECFDRPALECFLNDAGITTGRHYPFAVHQQPAYAGKIRGSQTLP
                     VTDALYRHLVSLPCFPELRDDDVTHISETLLRWSSHSRAKQRHVISCTGERVIR"
     gene            5939..>8705
                     /locus_tag="VLL06_10185"
     CDS             5939..>8705
                     /locus_tag="VLL06_10185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSS31375.1"
                     /translation="MTRYYCTYFDRQYLVKGLALIDSLQQHSGDDFKVFVVCADELTR
                     LLLTKLAIPQVITIPLHQLERGDSALLATKQTRSLVEYYWTLTPTVILRILEQHPTID
                     VLTYLDSDLYFFSSPAPIFEELGSGSILIHAHRFSPDQAHLGDANGTYNVGLLSFRRD
                     VRGMDALRWWRERCLEWCYARAEAGKMGDQMYLNDWPTRFNGVRELQHHGGGVGPWNH
                     DRYTFRTEGQGHVFVGDLPVIFYHFHSCTFVTPHIILPVKHPHYPLPLSALQYCFIPY
                     VCALEKAATAVQAIVPEFNYGLHTGESVTAAHTFFAKHAHAAQLDAMKLPQTRLRLCE
                     DWDLYCSAQVIHDRERVPSGQGSVAVSERITCKTGPQANTSPSPLQVLLEIAKDLAML
                     AHQLSELGHDSSKLGNIVAGWQRDLMVRRPDSPVCQLISTLPPICVAMLPSRLKLALG
                     YCEPQLQNILDWLAVSKETTNLTYDLTVLNKAHLQWFIALVTGTPGATIARYFEELEQ
                     DGRLTTHVRRLTLASQQRFTADEIARYGRRLGWYAIVRAIKPKVIVETGVDKGLGTCV
                     LAAAVMKNQAEGWPGHVYATEIDPKGGFLLQSPYTQFGTILYGDSIQTLQKFPHPIDL
                     AVCDSAHTAEYERGEYQAIRDKLRNGALVISDNAHVTDELADFATATGRQFLFFQEKP
                     AAHFYPGAGIGIAFQGLRIPNTTGARDASQKSGPATTVGTYFPRNQEEECQVQGQKRR
                     QDSDLMPLQHPLVSVIVSSYNAEAFMAECLEDLERQTLIGRMEIIIIDAASPQNERVV
                     IDAFQQRYHNILYLRTPKRIGVYAAWNMALKLATGTYVTPFSTNDRLRHDAYEILVRA
                     LDEHSDVALVYGDTYLTRMPHQTFEQHQREGIWQWPAYSYSDLRRRCLVGPHPMSVTF
                     ISIRSAVA"
BASE COUNT         1861 a         2475 c         2414 g         1955 t
ORIGIN      
        1 tgcatgatgg aatcagagca tataccagtc cattcgtcgg ttctcgtggt cggattgccg
       61 ctacagctgg tcatcggctc cggtgaagac aaagtgaact gtggatccac gttactgggt
      121 tggaaggagc gagcctggct gatttgcgaa tggccgttcc atttcgggca ggcggttccg
      181 tgcgaaacgg gaacccactg tctcgtccgt gctatggtgg ccggccggct tgtcgcgtac
      241 caaagcgaag tctgtatggt acagatgtct ccgcttcctc ttctgtatct cgcatttccc
      301 aggaggattg aggaaatcca tctccgtaag gatgcgcgtg tccctagcaa cgaaccgttg
      361 ctgctgatgc aagggaccca tagagacgcg atgctggccc tgcggaatgg gtcggctccc
      421 atcggcggat tgctgcaaga tgtcagcctg tcaggctgcc gaatcatcct acagaggtcg
      481 cgcactgacg tggtgttggg cgccacggtc aatctagaat tcgaactgat cgggatcgga
      541 cacgtcacgc atctcgccgg cgtgatcaag aatattgccg aacgtgacgg tgccatctct
      601 attggaattg agttccgctt caacggaaaa gaggccatcg agtaccgcgg gtggggaggg
      661 agcgtccaga aggccattga gtattccgtg atgcagcggc aaactgattt tggattgttg
      721 acgccgccgt cagacccatg acgaatctac cgagcactag gcacttcttg cctggcagaa
      781 gctctctttt cggtacccgc ttccccagca acccgctatc cactgcgtat ttctctccct
      841 gtgcgatttc ttcgcgagtg gcgcaagcct tgcttggctt ctagtcgaac attccgcatc
      901 ggcggcgatt gcgtgaaccc gagcatgacg ctcagcaggc ggatcttgtg tttgcatggc
      961 ctatgacagc catccatttg cgaataaggg aatacggtgc cacaagcgat cgaagtatca
     1021 actgatatgg gcgccctgct gaattctgca cgagcggtat ttcgagaaat cccgatgccg
     1081 cttgctgcct gcctggcgct gtggagacgg gcggcggccg gtggttccgg ctcagatcct
     1141 ctcgcgccga ggtcagccat gtcccacacc tatccttcaa cgtctcgccc cgagattgca
     1201 gcagttctgg aaggccttcc gttgggcgaa tgggcgatga ctatggcggc tatcgacttt
     1261 ctcctgaacc aggtcgaacg aatccgtccg catgccatcc tcgagttcgg cagcggtgcc
     1321 agtacgctcg cattggcatg catgatgcga aggttgcacg gtgcgtcgaa gaccccgcat
     1381 atctttagcg tggagcaatc tccgcgatac gttcagcaga tcgaatcccg cctccggctc
     1441 catggactgc aagagagcgt gcgatttatg accgctccgc tgatatccca ggtcatccag
     1501 aatgtgggga cagactgcta tcaattacgc gatcaggatc tccgcacgtt ctttgggcag
     1561 agcaaacccg atctcgtcgt ggttgacggt cctgcagcgg actatggagg tcgcttcgga
     1621 acgattccac ttgtcagaaa ctttctctcc gatcacgcct ggatttacat ggacgatgcc
     1681 ttacgagaca gcgaactctc catcgccgat tggtgggacc gtctcggcta tgtgaaggac
     1741 tgcggcgtcc tctggttggg caagggattg ctcactgctc ggctccattc gcccgagttg
     1801 ctggcccacg agcctgcaca tgtgtttctg cgccagcttc ttgaaccatc tgagccagga
     1861 catcgtcatc acattcacga actgccgcca gcggcttcgc ttgttcctgc tccattccca
     1921 tgcgagcgca tcgcagcgag cgacctgcgg gaggaggctc aggaactttc gccgccatca
     1981 ctcactggcc gcccgacctg tctttttctg aatacctatt attcaggttt tctgactcat
     2041 cactaccgtt cgatgtccga gcgagcgacg cagtcgtacg aagcacaacg gcgctcactc
     2101 cagcaaacct gctttggtga cagcgatttc tactcgtccg ggatgatgct ggcaggctgg
     2161 caggccgaag atatgatcgt gaattgtgcc ccccttcagg aagcctgggc gcgggagcaa
     2221 ggctatcgtg gtcacagaca gctgttggat attgcgatcg aacagattcg acgacgacgg
     2281 cctgaggttc tctaccttca ggacctggga ctggctacca aagagtttct cgcgtgtatc
     2341 cgtccgcatg tgacgctcat cgtcgggcaa attgcgtctc ccgttccagc ccatgcgaaa
     2401 ctcaagggat tcgatctgct cttttcctca tttccacatt tcgtcgaaga gttcagaaaa
     2461 caggggatga cagcctacta tcagccgttg gcctttgagc cacgcatcgc tcacattcct
     2521 gcaccatccg atcgtgcaca cgccgtcacg tttgtgggcg gcctctctgc tgcgcacaag
     2581 gaacgacagc aattcatcgc ctcgcttgcg aagctcgttc cactcgaatg ttgggggtat
     2641 ggcgtcgatg ctctgcgtgc ctcaggaatc gatcagacgc gtctgcacgg ggaagtctgg
     2701 ggggacgaga tgttctccac acttcgacag tccctgatca ccatcaatca tcacacagat
     2761 gtcgcgcaga cctacgccaa taacatgcgg ctctttgaag ccaccggatg cggtgcattg
     2821 ctggtgacgg atcataagga caacctcgat gatctgttta gaaccgggga agaggtcatt
     2881 gcttaccgtt cgctggacga gtgcgcagcg ctggttcgct attacatcgc ccatccggag
     2941 gaagctcgcc gcatcgccga ccgagggcag gctcggacgt tccgtgacca cacctatgcg
     3001 agacgcatgg cccacacagc ggagattgtg agccggcatc ttgaggaacg acaaggccat
     3061 cagcgccttc ccgatccaga tctcgcacac gtatcgtatg gcaaagaacc gatccagccg
     3121 gatgagatca ccacagcctt ggcgacatcc tggcaatccc cgtcgattcc cctcaaacag
     3181 cgagcgctgg tgcagcaaga attaaccgct ctctatcagg gaggtccacc agtggtctat
     3241 cggatcttgg ccgatgccct gcggcccttc gttgcgccgg attgttctgt gctcgaaatc
     3301 ggttgcgcga gcggctacta ctatgaagtt ctggagtatc tcctcaatac caggctctcg
     3361 tatgtcgggg ttgatttctc gcctgcgatg gtggccttgg cacaacgcta ctatccacgg
     3421 gccatcttcc atgtagggaa tggagcggcc ctcaacttcc ctgatcagag ctttctcatc
     3481 gccatctctt cttgtgtact cctgcacgtt gccgactatt cgcaacatat tcgggaagcg
     3541 gcacgtgtgg cttcgcagat cgtggtgctg caccgaacgc ctatttgccg agcccatccc
     3601 acaaatcatt acaaaaagtt cgcgtatggg gttgaaacct atgaactgcg attgaacgag
     3661 tcagagatcc tcggcctgtg tcgcgatcaa aatctcgagc tggtccagcg attggaatat
     3721 gacagccacc ccgagcgaga tgaatttgaa gcgacctatg tctttcgaaa ggtcggccgt
     3781 tatggatagg gggtttcgtt cagccaaggt gttgatcaca ggaggcatgg ggtttatcgg
     3841 atccaacctg gcacatcggc ttgtgaccat gggtgcggag attaccctgg tagacagcat
     3901 gattccggaa tacggcggca acctttggaa cattcaagaa atcaggaatc acgtacaggt
     3961 caacatctcc gatgtgcggg acgagcattc gatgaagtat ttgctccaag gctgcgatta
     4021 cctgttcaac ctcgcgggcc aaacgagtca tgtggactct atggaacacc cgcagccgga
     4081 cctcgagatt aatgcccgcg cgcagctgtc catcctcgaa ggctgtcgcc gccacaaccc
     4141 gaatatcaaa gtcgtatttg ccagcaccag acaaatttac ggacgtcccc agtacgtgcc
     4201 ggtcgatgag ctgcatccca ttcagccggt cgatgttaac ggcatcaaca agatggcagg
     4261 agagatgtat cacttgctct attgccgggt gtacgggatt cgaaccgccg tgcttcggtt
     4321 gaccaacacc tatggcccac ggatgcgagt gaaagatgcc cgtcaaacat ttcttggagt
     4381 ctggattcgc cgactcatcg agggccagcc catcttggtc tacggagatg gaacacaaat
     4441 tcgagacttt aactatgttg aggatgtgat tgacgcgctg ctcctggccg cttcgacaga
     4501 caaggccaat ggcgaggtct tcaacctggg agccggcgat ccgattagtt tggaagacac
     4561 ggccaagttg ctcatcacga tatatgggaa aggatcctac gagctgatcc catttccgtc
     4621 cgagcgtcaa atcatcgaca ttggcaatta ttacgggaac tatcacaaaa tccagtcaca
     4681 gcttggctgg caaccgaaga ccttccttcg tgcaggggtg gagcagacac tcacctttta
     4741 cgggcgctac tgcatgcact actggcagga tgtccatgct gcgtaaaccc attcctctcg
     4801 cagatccagg cgcgcagatg cgactctgcg gggagtccat gcgatcggcc tttgatcgcg
     4861 ttctgaccag tgggcggtac attctcggca atgaggtcga ggcgtttgag cgtgagtggg
     4921 cggcttatct tcgagcgcgg cactgtattg gagtcggaaa tgggaccgat gcccttgcgt
     4981 tggcgttgct ggctgccggc gtaaaggcgg gagaggaagt gatcaccgta tctcacacgg
     5041 ctgtggcgac cgttgccgcc atcgagcaga tcggtgccgt ccctgtattc gccgacatcg
     5101 acccgagctc tcgctgcatg gaccccgccc atctacccgg actcatttct gaaaataccc
     5161 gagccatcgt ccctgtgcat atgtacggcc agccggctcc gatggggaag atcatggccg
     5221 tggcgaagga gcatgggctc aaagtcatcg aagattgtgc acaggcccat ggagcgacgc
     5281 ttgggggaca atacgtgggg acgttcgggg atgccgccgc attcagcttt tatccaacga
     5341 agaatctcgg cgcgctcgga gatgcaggag caatcgtgac caacgatgca actctggctg
     5401 cttcgctcag gatgcttcgg gagtatggat ggcaggaacg ctatgtcagt ctcacgcccg
     5461 gcagaaactc gcgactcgat gaacttcaag ccgccattct tcgggcaaaa ctaccggacc
     5521 tcgaccgcca taatatccgg cgtcgcgcga tcgccgccgc ctacacgcag gccttgcgga
     5581 acaccgccct tgtggctccc gcccagatcg agaggacgga acatgcgatg catctctatg
     5641 tcctggaatg tttcgatcgt ccggcgttgg agtgcttcct gaacgatgca gggattacaa
     5701 ccggtcgcca ttacccattc gccgtacacc agcagccggc ctatgccgga aaaattcgcg
     5761 gcagtcagac gttgcctgtc accgatgcac tctatcgaca tctggtgtct ctgccctgtt
     5821 tccccgagtt gcgggacgac gacgtcacac acatctcgga gacgctcctc cggtggagca
     5881 gccacagtcg ggccaaacag aggcatgtga tcagttgcac aggggagagg gtaattcgat
     5941 gactcgctac tactgcacct acttcgaccg tcagtatttg gtcaaggggc ttgccctcat
     6001 tgattccctc cagcaacatt cgggcgacga ctttaaggtg ttcgtggtat gtgcggatga
     6061 actcaccagg ctcctgctca cgaagttggc catcccgcaa gtcatcacca ttccgctgca
     6121 tcaactcgaa cgaggcgaca gcgccttact cgccaccaaa cagacccgtt ccttggtcga
     6181 atattattgg acactcactc ccactgtgat cctgcgtatt ctggagcaac atccgaccat
     6241 cgacgtgctg acctatctcg attcagatct ctattttttt tcgtcgcctg cccccatctt
     6301 tgaggagttg gggtccggct ccattctgat tcatgcacac cgcttctcac cggatcaagc
     6361 gcatctcggt gatgcgaacg ggacgtacaa cgtcgggtta ctttcgttca ggcgagatgt
     6421 tcgcgggatg gacgcgctgc gttggtggcg ggagcgctgt ctggaatggt gctatgcccg
     6481 tgctgaggcc ggcaagatgg gcgaccagat gtatctgaac gattggccta ccagattcaa
     6541 cggcgtgaga gagttgcagc atcatggtgg gggagtggga ccctggaatc acgatcgata
     6601 cacgttcagg acagaggggc aagggcatgt atttgtgggc gatcttcctg tgatctttta
     6661 tcatttccac tcgtgtacct tcgtcacgcc tcatattatt ctgccggtga agcacccaca
     6721 ttatcccctt cctctttcag ctctccagta ttgtttcatc ccatacgtct gcgcgctgga
     6781 gaaagctgca accgcggtgc aggccattgt tcctgaattt aactatgggc tgcacacggg
     6841 tgaatccgtc acggcagcgc ataccttctt tgccaaacat gcacatgccg ctcaacttga
     6901 tgccatgaaa ctgcctcaga ctcgcctgcg gttgtgtgag gactgggacc tgtattgttc
     6961 cgcacaggtg atccacgaca gggaacgtgt gccgtcagga cagggaagtg tggccgtgag
     7021 cgaacgaatc acatgcaaga ccggccctca ggcaaacact tcaccatcgc cacttcaagt
     7081 attacttgag attgccaagg atctcgccat gctcgctcat caattatccg agctgggaca
     7141 cgacagcagc aaactcggaa acatcgtcgc tggctggcag agggacttga tggttcggag
     7201 gcccgattca ccggtctgcc agttgatcag cacgttacct cccatctgcg tggccatgct
     7261 cccgtcccgc ttgaagctgg cgttggggta ctgtgagccg cagctccaga acatactgga
     7321 ttggctcgca gtctcgaagg aaacgacgaa cctcacgtat gacctcaccg tactcaacaa
     7381 ggcgcacctc cagtggttca ttgcgcttgt gacggggacg cccggcgcga cgatcgcacg
     7441 ttacttcgaa gagctggagc aagacggacg gctcacaaca cacgtgcgcc gtctgacgtt
     7501 ggcgagtcag cagcggttca ctgccgacga gattgctcgc tacggcagac ggctcgggtg
     7561 gtatgccatc gtccgtgcca taaaaccgaa agtcatcgtg gaaacaggag tcgataaagg
     7621 gcttgggacc tgcgtgctcg ctgccgctgt gatgaagaat caggccgaag gttggcctgg
     7681 gcatgtctat gccacggaga tagaccccaa aggcggtttt ttgctccaat ccccgtacac
     7741 ccagtttgga acgatcttat atggagactc tatccagacc cttcagaagt tccctcaccc
     7801 gatcgacctc gccgtgtgtg acagcgcgca cactgccgaa tatgaacgag gggagtacca
     7861 ggccatacgg gacaagctgc ggaacggggc gctggtcatc agtgacaatg ctcatgtgac
     7921 agatgaactt gccgactttg cgaccgctac tggtaggcag tttctgtttt ttcaagagaa
     7981 acccgcggcg catttctatc ctggggccgg cattggaatc gcgttccaag gactaagaat
     8041 tcccaatacc acgggggcga gagacgcatc gcagaagagc ggtcctgcca cgactgttgg
     8101 tacctacttt ccacgcaatc aagaggagga atgccaggtg caaggacaga aacgaagaca
     8161 ggactctgat ctcatgcctt tgcaacaccc cttggtatcg gtcatcgttt cctcctataa
     8221 cgccgaggca ttcatggctg aatgcctcga agaccttgag cggcaaaccc tcatcggtcg
     8281 catggaaatc atcatcattg atgccgcatc ccctcagaat gaacgagtgg tcatcgatgc
     8341 attccagcag cgataccaca atattctgta tctccgtacc ccgaagcgta tcggtgtgta
     8401 tgccgcctgg aatatggcct tgaagctagc gaccggcacg tatgtgactc cgttcagtac
     8461 aaacgaccgg ctccgtcacg atgcgtacga gatcttggta cgagcactcg atgaacattc
     8521 ggatgtggcg ctggtctatg gcgatacgta cttgacgaga atgccacatc agacctttga
     8581 acagcatcaa cgcgagggga tctggcaatg gccggcgtac agctatagcg atctgcggcg
     8641 gcgttgtctg gtaggcccgc atccgatgtc tgtgacgttt atttctatca ggtcggcggt
     8701 ggcaa
//
LOCUS       DATIPF010000409        13244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_784392, whole genome shotgun sequence.
ACCESSION   DATIPF010000409 DATIPF010000000
VERSION     DATIPF010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 13244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13244
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784392"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /gene="larC"
                     /locus_tag="VLL06_10190"
     CDS             <1..206
                     /gene="larC"
                     /locus_tag="VLL06_10190"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HSS31376.1"
                     /translation="RFISVKVRGGVVRMKVADVNATTAKAAPEYLDCKRIAERTGRPV
                     KRVLEDAALAYAKQREARPSGQR"
     gene            203..1207
                     /locus_tag="VLL06_10195"
     CDS             203..1207
                     /locus_tag="VLL06_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31377.1"
                     /translation="MTVLLYSLLFLVSVAVTLGACTLFTNAIEWVGKRFNLSEGAVGG
                     VLAAIGTTLPETSIPIIAIFFGASRAEVEVGLGAILGAPFMLSTLVIPILAILLVVYA
                     GLGKRTAAFQLKYRDVKGDLIFFVVAYSAALACVFIPSQLVHAIAAVGLIGLYLYYVK
                     MKFSETDEEGGENGVDPLYFSRKSPVPSFGLIGLQGILGLAGLAFGAHLFVVSAETIA
                     GALSISPLILALLVAPLATELPEMSNSFLWLYRKKDRLAVGNVTGAMVFQGTIPVSIG
                     LLGTDWALAPTALITMVLAVAAATFLLAQAVWGGLWRPWLLGGSALLYIGYAVYLYGW
                     "
     gene            1277..2311
                     /gene="pdxA"
                     /locus_tag="VLL06_10200"
     CDS             1277..2311
                     /gene="pdxA"
                     /locus_tag="VLL06_10200"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HSS31378.1"
                     /translation="MKFQRGQPKKQDKPNKPLLGITMGDPAGIGPEVIAKAMAGRALR
                     RLCRPMVIGSFPVMQHTVKSLKLKMQVIQVEGHEAVSSRGNHLAVLDPLDRPLGRFPR
                     GVAAPETGAASVLFIKKAVELAQLGCIDGIVTAPINKEAINMAGCHFPGHTELLADLT
                     KAQESGMMIIGGPLRIMFVTTHVAIKELPSLLTQDKIEKAIRLAHLALKDLFGIKKPR
                     VGVAALNPHAGEHGLFGDEEARVILPAARAAQAQGIRASDPLPADTLFGKAARGDYDG
                     VVALYHDQGLIPLKLVAFGTCVNLTVGLPIIRTSVDHGTAFDIVGKGIADPGSLVEAI
                     TLAAMLAKRR"
     gene            2420..2755
                     /locus_tag="VLL06_10205"
     CDS             2420..2755
                     /locus_tag="VLL06_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31379.1"
                     /translation="MTNQSDAPRDRLQEQLKELDQIFQDTMETLNIASGTERVLKWRV
                     ITVALITESVGQKEGQKFAALQPGPSFTNDLVEEFTDLIDYFRTPLAHLAKQQAQAAP
                     RPSGGTGGN"
     gene            2759..3556
                     /gene="rsmA"
                     /locus_tag="VLL06_10210"
     CDS             2759..3556
                     /gene="rsmA"
                     /locus_tag="VLL06_10210"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HSS31380.1"
                     /translation="MSTPDLPPPIKRLGQHFLIDPNIVRKILALAELNTSDHVLEIGP
                     GRGILTEALCRAAGRVTAIEVDPRLHAYLGSKQSGFPNVELVCEDALIYPVENLPMGT
                     VVVANLPYYISTPLLFRLLDQRDRFPRMVLMLQNEVADRLVAKPDGSDYGVLSVMAQY
                     AAEITKAFRVSAHCFRPRPEVGSAVVLLRTKERRTLSPEEEPKFAALVKASFAHRRKT
                     LINSLKDEGYDQKSVTEALQSLNLLPTARAEILSVAQFIELSDLTQR"
     gene            3724..6138
                     /locus_tag="VLL06_10215"
     CDS             3724..6138
                     /locus_tag="VLL06_10215"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HSS31381.1"
                     /translation="MAVSTSAKRGEARGAASSPSHIKREVIGVVLIALALLTLLSLLS
                     FVSGEVKDGTSTTPPSRNLIGSVGAFFASTLFWSIGAAAYLFPILLGLMGIRCFTQDD
                     LSIRLRNAGASLAALLFFSGLLHLEVTAVPTISSGLIYRGMAGGLFGRVLAEGLRAYF
                     ASTGAHILIMAGLLVSLLFTTSMSLAKMAQRIPGLSNWMFNRARALIPDRPVEESILE
                     SPRKAKQKSSKSIREVIEEALPEAAAEPELNWPVIQPSRQSTPEPVEPTESESEPQII
                     TAQSKAGDYILPDPAVLLSEPSGPLGRVTEEEIKAQSDILTRALLSFGIAGKVTEVRP
                     GPVVTMYEFEPAPGTKVARIVNLADDLALALKAISLRIVAPIPGKSVVGIEVPNLYRE
                     TVSMKEVMTSDAFSRARSKLSLALGKDIFGAPTIADLKTMPHLLVAGATGAGKSVSLN
                     TMLLSLLFSARPDEVKLLLIDPKMLEFQTYDGIPHLLRPVITDPKSAARGLSWVVAEM
                     ERRYKLLAEAGVRNIDTYNRKVAGGQGVLEAESAAKLDQPELPIQFLPEEERLSAGET
                     AIPDGSPGSFAPPQTPPEPLPYIVVMIDELADLMMVAPREVEDKIARLAQMARASGIH
                     LVLATQRPSVDVLTGLIKANFPARIAFQVSSKTDSRTILDANGAEALLGRGDMLYLAS
                     GTGRVMRLHGSFVSDDDVRRVVEFVKEQATPAYNQELLVASKQEVAKEEDSLGEVYEQ
                     AKELVLSTGQASASLIQRRLRVGYPRAARMIEQMEVDGLVGAAGRDGRREVVGRRGPV
                     GAEEGA"
     gene            6250..6984
                     /locus_tag="VLL06_10220"
     CDS             6250..6984
                     /locus_tag="VLL06_10220"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HSS31382.1"
                     /translation="MMKKLNWACIVGGVLVCAWSLFPLFAFAAADEGDEKALGEVREV
                     VKQLQARYEKTKDLQADFTQKTIIEGFERPITSSGKVYIKKPGRLRWNYLDPSVEDIY
                     VNRDDIKVYVPEHKQVLVGKLTQMAASKAPLELLQGAAKLDESFDIEPTPGKGRGVGG
                     IRLLTLLPKSREGEVGRSLQRIVLEVFPKTYFIRTISLYEVSGNVASFEFSSLQPNMG
                     LGDSIFDLKLPADVAVMKAPVLSTPQ"
     gene            7041..7805
                     /locus_tag="VLL06_10225"
     CDS             7041..7805
                     /locus_tag="VLL06_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HSS31383.1"
                     /translation="MNQSMVRSLGEAVDDAVKQLDFDRLSREYWDQNEFLVIPQFLTR
                     SVVEEHLVPQAQGVKSELNRNFIPGHKKGGSVSYYTVIEKAPRFLDLYRSSAFIEFLS
                     RLVQAKLSLCPENDPHSCALYYYTEPGDHIGFHYDTSYYKGARYTILMGLVDRSTQCK
                     LVCELFKDDPVKSPKHLELITQPGDLVIFNGDKLWHSVTPLGEGEERIALTMEYVTNP
                     DMGTFKRLYSNLKDSIAYFGLRAVFKQAFTPSNRRS"
     gene            8181..9245
                     /locus_tag="VLL06_10230"
     CDS             8181..9245
                     /locus_tag="VLL06_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS31384.1"
                     /translation="MNRLLQYSAIVLFALCLAVVSPVALVSSAAEQPDAAQRHSERGI
                     TLLRKGDLAAASEAAKKAIELNPSSAEAHHLLGMIALKEKKPAQAVDAFTHALKLKPA
                     YPEALNDLAEVYRIQGKVTEAEQALKRAIEIDPSHADSSFNLAKLYEQRREFAAATQT
                     YQTLLATHPRHLDALYNLALLYEARGDSRAARETVSRLLKLDPKYADAWYLAGRMAEK
                     NNALEEAAHSYRQAIAEKPALVDAHYNLAFIYRSQGRSVEAERELLEVLRYRPEYAEA
                     HMNLGVIYTGLNRLEEAEHSYERAVALKPDYAEAHYNLGVFYELHRKDLPNALAQYHA
                     YLNLGGRDDRVERIVGSGGR"
     gene            complement(9623..10162)
                     /locus_tag="VLL06_10235"
     CDS             complement(9623..10162)
                     /locus_tag="VLL06_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HSS31385.1"
                     /translation="MPLSALALLVTLTGCTSMQEAGDGSEAKALTFEQVKTAPYSVKG
                     QTAVFGGKVLTARLLKEGTRIEILQLPLDRSNRPTYDLTRSQGRFIAFQREFLDPATL
                     PPGTKVTVTGEVSSSITLPLDETDYTYPVINIKQLRVWDRSDDAASRLRTHPYTVGPY
                     WGGYRPLFWGNPWGWGWPY"
     gene            10256..10906
                     /locus_tag="VLL06_10240"
     CDS             10256..10906
                     /locus_tag="VLL06_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSS31386.1"
                     /translation="MMNRQCASWASAVCAVVWSVVVLWGSLATAASPELKGKFEILKG
                     EVSTHTPGKVKLIEFADFYCPHCHRFDEEALGSLEKEFGNKLETTMVGFPVIHGKLPT
                     PFDMYEQAKMMGKGKEMKAVLFRTIHTDKITGVLDRSIREVLIKEVGLDPKVFEEEMA
                     SGKAARIFEDGRKWGERIKVQSTPTVLLDGNIKAETIDPENLKLIIQSILDADRKK"
     gene            11038..>13244
                     /locus_tag="VLL06_10245"
     CDS             11038..>13244
                     /locus_tag="VLL06_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HSS31387.1"
                     /translation="MAIDPICGMTVDPATAAGSHDHRGQRYYFCGLSCLERFKADPER
                     ALQPQLVSSAKPTTTRKPLPMMQPAPAVMGPTAIDPVCGMTVQPATAAGSYEYQGKTY
                     YFCATSCLTKFRADPVHYLTPPEQRASRGMPIPSGGVVEYICPMDPEVLETQPGACRI
                     CGMALEPKVVSLEDVGNPELEDMTRRFWLCLVPSLLVMLLAMAEMIPGVSLPQALTGS
                     MRNWVQWLLATPVVLWGGWPFFHRGWTSVVNRAPNMFTLIAMGTGAAYGYSAVATVAP
                     TLFPSSFRMHDGSLAVYFEAAAIITVLVQLGQVLELKARSQTSSAIRALLRLVPKTAR
                     LIKPDGQEEDVPVEHIQMGHRLRVRPGERVPVDGMVQEGRTAVDESMITGESIPVEKT
                     VGERVTGGTINGTGGLVMVAERVGRDTMLARIVQMVGEAQRSRAPIQRLADVTAGYFV
                     PLVVAVAVITAVAWAIWGPEPALAYALVNAVAVLIIACPCALGLATPMSIMVGTGRGA
                     MVGVLVKKAEALEVFGKVDTLVIDKTGTLTEGKPKLLSVHVVPPLSEVDLLRFAASLE
                     RSSEHPLAAAVVAGAEGRGIVCAPVEQFRSVTGKGVTGTVTGRRVAIGTAAFLRNDVG
                     VSDHSLTQLDDEVASLRREGQTILFVVIDEQVAGVLGVADPIKDSTRDAVQELKAEGI
                     RVVMVTGDHRDTAEAVARQLGIDEVMAGVLPEQKGQIIARFKSEGRVVAMAGDG"
BASE COUNT         2754 a         3494 c         4056 g         2940 t
ORIGIN      
        1 ggcgatttat ctcggtgaag gtgcgtgggg gcgtggtcag gatgaaggtg gccgatgtga
       61 acgcgacaac agccaaagcg gctcccgaat atctcgattg caagcgaatc gccgaacgaa
      121 cgggccggcc tgtgaagagg gtgctcgaag acgccgctct tgcctatgcc aaacaacggg
      181 aggctcgccc gtcgggccag cgatgacggt cctcctctac agtctcctgt tccttgtctc
      241 cgtcgcggtg acgttgggtg cctgcacgct cttcacgaac gccattgagt gggtaggcaa
      301 gcgatttaat ctgtccgaag gcgcggtcgg cggtgtattg gcagccatcg gcaccacact
      361 tcccgaaacg tcgattccta ttattgccat cttcttcggg gccagccgtg ctgaggtgga
      421 ggtggggttg ggcgcgatcc ttggcgcccc cttcatgctg agcacgttgg tcattcccat
      481 tttggctatc ctgctggtgg tctatgccgg acttgggaag cgaaccgccg cctttcaatt
      541 gaaatatcgc gatgtgaagg gcgatcttat ctttttcgtg gtggcctaca gcgctgcgct
      601 ggcctgtgtc tttatcccgt cccaattagt ccatgccatt gccgccgtcg gattaatagg
      661 cctctatctc tattacgtca aaatgaagtt cagtgagacg gatgaggagg gaggcgagaa
      721 tggggttgac ccgctgtact tttccagaaa gtctcccgtc ccgtcgtttg gactcatcgg
      781 ccttcaggga atccttggtt tggcggggct tgcgttcggc gcgcatctgt tcgtggtctc
      841 cgcagaaacc attgcgggag ccttgtcgat ttctccgtta atcctggccc tcctcgtggc
      901 gcccctggcc acagagttgc ccgagatgtc gaatagtttt ttgtggcttt accggaagaa
      961 agatcgtttg gctgtaggga atgtgacagg cgcgatggtc tttcagggta ctattccggt
     1021 ctcgatcgga ctcttgggca cggactgggc actagcaccg acggccttga tcaccatggt
     1081 gctcgctgtg gctgcggcga ccttcttatt ggcacaggct gtgtggggcg gtctctggcg
     1141 accctggttg ctgggcggga gtgctctcct ttatataggg tatgcggtgt acctatatgg
     1201 ttggtgacca gttgaaaagg tttgtctggt ttttctggtt tgtttcgttt gtttggtttc
     1261 aacgaggagc gaatcaatga agttccaacg aggccaacca aaaaaacaag acaaaccaaa
     1321 caaacccctt ctggggatta ccatgggtga tccggcaggt ataggaccgg aggtgatcgc
     1381 gaaagccatg gcgggaagag ctttacggcg cctgtgtcgg ccaatggtta ttggctcctt
     1441 tccggttatg cagcacacgg tcaagagtct gaaactcaag atgcaggtga ttcaggtgga
     1501 ggggcacgag gccgtgtctt cacgggggaa tcatctcgcc gtgttggacc cgctcgatcg
     1561 tccccttggg cgatttccgc gtggagtggc ggcgcctgaa accggggcgg cgtccgtgct
     1621 gtttatcaag aaagctgtgg agcttgcgca gcttggctgc atcgacggca tcgtcaccgc
     1681 accgatcaac aaagaagcaa tcaacatggc cggctgtcat ttccccggtc atacagaatt
     1741 gctggcagac ctgactaagg cacaggaatc cggcatgatg attatcggcg gaccgctccg
     1801 aatcatgttt gtgaccaccc acgtcgcgat caaagaattg ccgtcactcc tcactcagga
     1861 caagatcgag aaagcgatca ggctggctca tctggcgttg aaggatctgt tcggaatcaa
     1921 gaaaccgaga gtcggtgtcg cggcgttgaa cccccatgca ggcgagcatg gactgttcgg
     1981 agacgaagag gcgcgggtca ttctcccggc ggcccgtgca gctcaggccc agggtattag
     2041 agccagcgat cccttaccgg ccgatacgtt gtttggcaag gcggcaagag gagattacga
     2101 tggtgttgtg gctctgtacc atgaccaggg gctcattccc ttaaaactag tcgcgttcgg
     2161 cacctgcgtg aatctgacgg tgggattgcc gattattcgt acctcggtcg atcacgggac
     2221 ggcgttcgat atcgtaggca agggcatcgc agaccctggc agtctggtcg aggcgattac
     2281 gctggccgcc atgttagcca agaggcggtg atgaacggtt tgtttggttt atttcgtttg
     2341 ccttgtttgt ttggttgccc gacacttcaa cgagacaaac gagataaacg aaatagacca
     2401 gaaaaacagg agtctactga tgactaacca atcggacgcg ccgcgtgaca ggcttcagga
     2461 gcagttaaag gagttggacc aaatttttca ggacaccatg gagacgctca atattgcttc
     2521 tggaactgaa cgagtgctga agtggagagt cataaccgtc gcgctcatta ctgagtccgt
     2581 ggggcagaaa gaaggccaga agtttgccgc cctccagccg gggccttcct ttaccaacga
     2641 cctggtagaa gagtttaccg atctcatcga ctatttccga actccgctgg cccatctggc
     2701 aaaacagcag gcgcaagctg ctcctcgtcc ttccggcggg accggcggga attgaccggt
     2761 gtcgaccccc gaccttcctc cgcccatcaa acggctcggc caacactttt tgatcgatcc
     2821 caacatcgtc cgcaaaatcc tcgcgctggc ggaactgaac accagcgatc acgtgttgga
     2881 gatcggtccc ggccgcggca tcctgacaga ggcgttgtgt cgcgcggcag gtcgtgtgac
     2941 ggcaatcgaa gtggatccgc ggctccatgc ctacctcgga agcaagcagt cggggtttcc
     3001 gaatgtcgag ttggtctgtg aggatgcgct gatctatccg gtcgaaaacc tgccgatggg
     3061 tacggtcgtc gtggccaatc tgccctacta catctcgaca ccgctgctct tcagattact
     3121 cgatcagcgc gatcgctttc cgcgcatggt cctcatgctc caaaacgaag tggccgatcg
     3181 tctggtggcg aaaccggatg gatcggacta tggcgtgctc tcagtgatgg cgcagtatgc
     3241 ggccgagatc acgaaagcct tccgcgtctc agcccattgc ttcaggccaa gaccagaagt
     3301 cggttctgca gtggtcttgt tgcggaccaa agagcggaga acactgagtc ccgaggaaga
     3361 accgaagttc gccgcgctgg tgaaagcctc cttcgctcat cgacgtaaga cgctcatcaa
     3421 ctcactcaag gatgaaggat atgatcagaa gtcagtgaca gaagcgcttc agtcgctcaa
     3481 cctcttgcca accgcccgtg cagagattct ctctgtcgca cagttcatcg aactgtcaga
     3541 tctgacacaa cggtaaggtt gatctcgaac ctgcgatgtc gcaggtggat tctgaagttg
     3601 aggcgatgac tggtcttacg gttgaatcat cgtcggaggc ccggcgaaga gaaaacaccc
     3661 tgtctcaaga cgcacccgcc tgattgtttg aaatgaccgt ttccctgtgc tagcatcgcg
     3721 atcatggcag tcagcacttc ggccaagcgg ggcgaggccc gcggcgccgc ttcctcccca
     3781 tcacacatca aacgagaagt gatcggagtc gtcctgatcg cgttggcgct cttgacactg
     3841 ctcagcctgc tgtcctttgt gtcgggagaa gtcaaggacg ggacttccac aacgcctcca
     3901 tccagaaacc tcatcgggtc ggtcggcgcg ttctttgcct ctactctctt ctggtcgatc
     3961 ggcgcggccg cctacttgtt tccgattctc ctcggtctca tgggtatccg ctgttttacc
     4021 caggacgatc tgtcgatccg tctgcggaac gccggcgcct cactggcggc cctcttgttt
     4081 tttagcggct tgctccatct cgaagtcacg gcggtgccga ccatctcaag cgggctcatt
     4141 tatcgtggga tggcgggagg cctcttcggg cgggtgctcg ccgagggttt acgggcgtat
     4201 tttgcgagca ccggtgcgca tatcctgatc atggcggggt tactggtgtc gttgctcttc
     4261 acgacatcca tgtctctggc gaagatggcg cagcggattc ccggtttgag caattggatg
     4321 ttcaacaggg cgcgtgcctt gattcctgac cggccggtgg aagaatccat tcttgagtcc
     4381 ccacggaaag ccaaacagaa atcgtcgaaa tctatccgtg aagtgattga agaggcgctt
     4441 cctgaggcgg ctgcagaacc ggaattgaat tggccggtga ttcagccgtc acggcaatcg
     4501 actcccgagc cggttgagcc gactgaatcc gagtcggaac cacagatcat cactgcccaa
     4561 tcgaaggcag gggactatat cttgccggat ccggccgtgc tcttgagcga gccgtcgggc
     4621 cccttagggc gtgtcacgga agaggagatc aaggcgcaat ccgatatcct gacccgtgcc
     4681 ctgctcagtt tcggtattgc agggaaggtc acggaagttc gccccggacc agtcgtgacg
     4741 atgtacgagt tcgagccggc gccggggacc aaagtcgcgc gcattgtgaa ccttgccgac
     4801 gatctggcgt tggcgctgaa ggcgatcagt cttcggattg tcgcgccgat tcccggaaaa
     4861 tcggtggtag ggatcgaggt gcccaacctc taccgcgaga cggtgtcgat gaaggaagtg
     4921 atgacgagtg atgcattttc acgcgccaga tcgaagcttt ccttggcctt agggaaggac
     4981 attttcggag cccccacgat cgcagatctg aaaacgatgc cgcacttgtt ggtagccggt
     5041 gcgacaggag cagggaaaag cgtcagtctc aataccatgc tcctgagcct gctgttttcg
     5101 gccagacccg atgaggtgaa gctgctcctc atcgatccca agatgctcga atttcaaacg
     5161 tacgacggca ttcctcatct gttacggccg gtcatcaccg atcccaagtc ggcggcgcgg
     5221 ggactcagtt gggtggtggc ggaaatggag cggcgctaca aattacttgc tgaggcaggg
     5281 gtgcgaaata tcgataccta caatcgaaag gtcgccgggg gacagggtgt gctggaggcg
     5341 gaatcagcgg ccaagttgga tcagccggaa ttacccatcc agtttttacc tgaggaggag
     5401 cggctctctg ccggagagac cgcgattccg gacggcagcc ccggatcctt tgccccgccg
     5461 cagactccgc cggagccctt gccctatatc gtggtgatga tcgatgaatt ggcggatttg
     5521 atgatggtcg cgccgcggga agtagaagac aagatcgcgc gtttagcgca gatggctcgc
     5581 gcatcaggca ttcatctcgt gctggcgacg cagcgtccat cggtcgacgt gctgaccggc
     5641 ttgatcaagg cgaacttccc ggcccgcatc gcatttcaag tgtcttcgaa gaccgattca
     5701 cgcaccatct tggacgcgaa tggtgcggaa gcccttcttg gccgcgggga tatgctctat
     5761 ctggcttcgg ggaccggacg tgtgatgcgg ctgcacggat cgtttgtgtc ggatgacgat
     5821 gtccgccgcg tggttgagtt cgtgaaggag caagccaccc ccgcttacaa tcaggagttg
     5881 ctggtcgcct cgaaacagga agtggcgaaa gaggaagact cgctgggcga agtctatgag
     5941 caggcgaaag agttggtcct gtctaccggt caagcgtctg cgtcgttgat tcagcgtcgg
     6001 ctgcgagtgg gctatccacg ggcggctcgg atgatcgagc aaatggaggt cgatggtctc
     6061 gtcggtgcgg ctggtcgcga tggacggaga gaggtcgtgg gtcggcgtgg gccggtggga
     6121 gcggaggaag gggcgtgagg gggagtggcc aggcgccctt cttgctcgca gaacgcgcac
     6181 gataagaatg tgctcgttcg atgagcgcag taaagggcag ccttggccaa tcccctcatg
     6241 atttgtatta tgatgaagaa attgaattgg gcatgtattg tagggggcgt actcgtttgt
     6301 gcgtggagcc tattcccatt gttcgctttt gctgctgcag atgagggcga tgagaaagca
     6361 ttgggggaag tgcgagaggt ggtcaagcaa ctccaggcgc ggtatgaaaa gacgaaagat
     6421 cttcaggcgg actttacgca gaagactatc attgaggggt tcgagcggcc tatcacgtcc
     6481 tcggggaaag tctatatcaa aaagcccggc cggttacggt ggaactatct cgacccttcg
     6541 gtcgaggaca tctatgtcaa tcgggacgat atcaaagtgt acgtgccgga gcacaaacaa
     6601 gtattggttg ggaagctcac acagatggcg gcttcaaagg ctccgttgga gctcttgcaa
     6661 ggggcggcga aactggatga atcgttcgac attgagccga caccgggaaa gggccgtggt
     6721 gtgggtggaa ttcggttact caccctcttg cccaagtcgc gtgaaggtga agtgggaaga
     6781 tcgctacaac gaatcgtact ggaggtgttt cccaagacct acttcatccg gacaatctct
     6841 ctctacgagg tcagcggcaa tgtcgcgagt tttgaatttt cgtcgctcca acccaacatg
     6901 ggtctcggcg actcaatctt cgacctgaag ttgccggccg atgtggcagt catgaaggcc
     6961 ccggtgctca gtactccgca gtgaaggtga cgagaacggc tccgaggatg catactgaag
     7021 attgaagggc aggtgagtat atgaatcagt caatggtccg ttccttaggc gaagcggtcg
     7081 atgatgcagt caagcaattg gatttcgatc gactttcacg tgagtattgg gaccagaacg
     7141 aattcctcgt gattccccag tttctcactc ggtcggtggt agaagagcat ctcgttcctc
     7201 aggcccaagg ggtgaagagc gaattgaatc gcaactttat tcccggccac aaaaagggcg
     7261 ggagcgtgag ttactacacg gtcatcgaga aggccccgcg ttttctcgat ctatatcgat
     7321 ccagcgcctt catcgaattc ttgagccgtc tggtgcaagc caaactcagt ctctgtcccg
     7381 agaacgatcc ccattcctgt gcgctctact actatacgga gccgggggac cacatcgggt
     7441 ttcactacga cacgtcctac tacaaggggg cgcgttatac cattctgatg ggactggtcg
     7501 ataggtctac gcaatgcaaa ctggtctgcg aactgtttaa ggatgaccct gtgaaatccc
     7561 ccaagcatct tgagctcatc acgcagcctg gcgatctggt catcttcaat ggagataagc
     7621 tctggcattc ggtgactccg cttggcgaag gagaagaacg gatcgccctc acgatggaat
     7681 atgtcaccaa cccggacatg ggcacgttta agcggctgta ttccaacctc aaagattcca
     7741 tcgcgtactt cggcctccgg gcagtcttca agcaggcctt cacgccttcc aatcgacgct
     7801 cttagcaggg cgctgaaaaa gcccgccagc ttcgttctcg gctcgtcgaa atcctcaacg
     7861 gggacccggc cgcctcaccg actcggcggc gcgcacagac gtggtgctcc ttattcgtcg
     7921 caccgtgcgc ccctgagggt acgcctccgg cttcaactcg cctgcggcct tgctgaacgg
     7981 actttttgag caccctgcga gagtgttctc tttttcttgt agacgtgcga accattgaat
     8041 ttctattgtg ccgcaatagt tttcccacag cccgctaaat gaggatggat ctcacgctgt
     8101 tacgcgcgtc gttgatttca tggttgctgc cgtctatagt ttgtcggact gttattgatg
     8161 cagaaaggcg tcggctgaga atgaatcgcc tcctccagta tagtgccatt gtgctgttcg
     8221 ccctgtgcct agctgtggtt tctcctgttg ccctggtctc gtccgcggct gagcaaccgg
     8281 atgctgcgca gcgtcactcc gaacgaggca ttacgctgct gcgaaaaggc gacttggctg
     8341 cggcatccga ggctgccaag aaagccattg agttgaatcc ctcttcagct gaagcgcacc
     8401 atctgcttgg catgatcgcg ctcaaagaaa agaaaccggc tcaagccgtc gatgccttta
     8461 cccacgcgct caagctgaag ccggcctatc ccgaggcgtt gaacgatctg gccgaggtct
     8521 atcggattca agggaaagtg accgaagcgg aacaggctct gaagcgagcg attgaaatcg
     8581 atccgagtca tgccgattca tctttcaacc tcgccaagct gtatgagcaa cgtcgtgagt
     8641 tcgccgcggc cacccagacc tatcaaacct tgttggccac ccatccgcgc caccttgacg
     8701 ccctttacaa tctcgcattg ctgtatgagg cgcggggaga cagtcgtgcg gcgcgagaga
     8761 cagtgagccg gctgctcaag ctcgatccca agtatgccga tgcctggtat cttgccgggc
     8821 ggatggcgga gaagaataat gcgctggagg aagccgccca ttcgtacaga caggccatcg
     8881 cggagaaacc cgcgctagtg gacgcgcact acaaccttgc gttcatttac aggagccaag
     8941 gacggtccgt tgaggccgaa cgggaattgc tcgaggtgtt gcggtatcgc ccggagtatg
     9001 ccgaagcgca tatgaatttg ggtgtgatct ataccggtct gaacagactt gaagaggcgg
     9061 agcattccta cgagcgcgct gttgcactaa agccggacta tgctgaagcg cattacaatt
     9121 tgggtgtctt ttatgagttg catcgaaaag acttgccgaa tgcgctggcc caataccacg
     9181 cctacctcaa tctcggtggg cgcgacgatc gcgttgagcg gatcgtcggg tctgggggac
     9241 gatgacgacg agatgagcag gatgaggagg tgaagagcct atcgacgaaa tcgttgaccg
     9301 ttgtggagat ctcctgaatg cctcggaagt ccgaatggag ccgaggcatt gctgtttcta
     9361 ttaggatttg gaaaggctat tttgacacgc gcgaatttcg ctggtcgccc gtgcctgcga
     9421 cgagttcagt cgaccgacgc aaccgtcttg cgcgccgggg gttgggcggg tgaaaaacgc
     9481 ggactttttc agcatcctgc cctacgccgt gtcgcaacgc attgcacagg aatatgaacg
     9541 agcctgccag cgccgagtca aagagcctga ttccaccagt attgacggga aactacgcgg
     9601 gattcctcaa gctggccatg ggtcagtatg gccaacccca accccatgga tttccccaaa
     9661 ataatggtct ataccctccc cagtagggtc ctaccgtata gggatgggtg cgaaggcggg
     9721 aagctgcgtc gtccgatctg tcccacacgc gtaattgttt gatattgatg acgggatagg
     9781 tatagtcggt ttcatctagt ggcaacgtga tggaactgga gacctccccg gtcacagtga
     9841 ccttggtccc cggtgggagg gttgcaggat cgaggaattc tcgttgaaag gcgatgaagc
     9901 ggccttgtga tcgggtgagg tcgtatgtgg gacggttcga tcggtcaagg ggtaactgca
     9961 agatttcgat tctcgtgccc tcttttagga gtcgggctgt caacaccttg ccgccgaaga
    10021 ctgccgtttg acctttcacg gagtagggtg ctgtcttgac ctgctcaaat gtgagtgctt
    10081 tggcttcaga accgtccccc gcctcttgca tcgaggtaca accagtgagt gtgacgagca
    10141 aagccagggc ggaaagcggc atacgcgtca gcatggccac agtataacag agagtaagtt
    10201 ttcgacacat tcgatataat gcgcggtccg tgttgatcca aaaaaaggag tggatatgat
    10261 gaacagacag tgtgcgtcat gggcctcggc ggtctgtgcg gttgtatgga gtgtcgtagt
    10321 cctctggggc tcactcgcca ctgccgcatc gccagagttg aagggcaagt tcgaaatcct
    10381 caagggtgag gtgtccaccc atacgcccgg caaggtgaag ttgattgagt ttgccgactt
    10441 ctactgcccg cattgtcacc ggtttgatga ggaagctctg ggctcgctgg agaaagagtt
    10501 tggaaacaag cttgagacga cgatggtggg gtttccggtg attcatggga agctcccgac
    10561 cccgttcgat atgtatgaac aagcgaagat gatgggaaag gggaaagaaa tgaaggccgt
    10621 cctctttcgc acgatccata ccgataagat cactggagta ctggatcgct ctatacggga
    10681 agtcctgatc aaagaagtcg gactcgatcc gaaagtgttt gaagaagaaa tggccagcgg
    10741 gaaggcggcc aggatattcg aggatgggcg taagtggggt gagcgcatca aagttcagtc
    10801 tacccccacc gtgctcctcg acgggaatat caaagcggag acgatcgatc cggagaatct
    10861 caagctgatc atccagagca ttctggatgc agatagaaag aagtgatcag tcacacgtga
    10921 tcggtcaggc gcgggatacg tggatcacgc aagcaacgat tgaagacatt cgaggagacg
    10981 catgcctgct tatccgtcgc gcctcatggc tcacgcctta cctgttacag tgttgtgatg
    11041 gcgatcgatc caatctgcgg aatgacggtg gatccggcta cggcagccgg aagtcacgac
    11101 catcggggtc agcgctacta tttttgcggc ctgtcctgcc tggagcgatt caaggccgac
    11161 ccggaacggg cattgcagcc acagctggtg agttccgcca agccgaccac cacaagaaaa
    11221 ccccttccca tgatgcagcc tgccccggct gtgatggggc ctacggcaat cgatcccgtg
    11281 tgcgggatga ctgtgcagcc ggccacagca gccggctcct acgagtatca aggcaagacc
    11341 tactactttt gcgcgacgag ttgcctgacc aagtttcgcg ctgatccggt tcactatctg
    11401 actcctcctg aacagcgtgc ttctcgcggt atgccgattc catccggtgg agttgtcgag
    11461 tacatctgtc cgatggaccc ggaggtgctc gagacccaac cgggagcctg ccggatttgc
    11521 gggatggcgc tggaacctaa agtcgtctcg ctcgaagatg tcgggaatcc cgagcttgag
    11581 gatatgacca gacggttttg gctctgtctg gtgccttccc tcttggtcat gctcttggcg
    11641 atggccgaga tgattcccgg cgtatccctt cctcaggccc ttaccggttc aatgaggaac
    11701 tgggtgcaat ggctgctggc gacgccggtg gtgctctggg gcggctggcc gtttttccat
    11761 cgggggtgga cctcggtggt gaaccgggct cccaacatgt tcacactcat cgccatggga
    11821 acgggagcgg cctacggcta cagtgctgtg gccaccgtcg cgccgacgct gtttcccagt
    11881 tcgttccgca tgcatgatgg ctcgctcgca gtctatttcg aggctgcagc catcattacg
    11941 gtgctcgtcc agctcggaca ggtcctggag ctgaaggctc ggagtcagac aagctctgcg
    12001 attcgtgcct tgctccgcct tgtaccgaag acggccaggc tgatcaaacc ggacggacaa
    12061 gaagaggatg tcccggtcga gcatattcag atgggccatc gattgcgagt caggccaggc
    12121 gagcgggtgc cggtcgacgg gatggtccaa gaagggagaa ccgctgttga tgaatccatg
    12181 atcacgggtg aatcgatccc tgtcgaaaaa acagtcgggg aacgggtgac cggcgggacg
    12241 atcaacggta caggtggact ggtgatggtg gctgaacgag tcgggcgaga cacgatgctt
    12301 gctcggatcg tgcagatggt cggtgaggcc caacggagcc gggcgccgat ccagcgtctc
    12361 gccgatgtga ccgcgggcta ttttgttccc ttggttgtgg ccgttgcagt gatcacggct
    12421 gtggcctggg cgatctgggg cccggagccg gcgttggcct atgcgctggt caatgccgtg
    12481 gcggtgctga ttattgcctg cccctgtgca ttgggattgg cgacgccgat gtcgatcatg
    12541 gttggaaccg gtcgaggggc gatggtcggt gtcttggtga agaaggctga agcgttggag
    12601 gtgttcggca aggtcgatac gctggtgatt gataagaccg gaaccctcac ggagggtaaa
    12661 ccgaaactgt tgtcggtgca tgttgtgccg cccctgtccg aagtcgatct gttgcgcttt
    12721 gccgcgagtc ttgaacggag cagcgaacat ccgttggctg cagcggtggt ggcgggagca
    12781 gaagggcgag ggatcgtgtg cgccccagtt gaacagtttc gttcagtgac ggggaagggt
    12841 gtgaccggga cggtcaccgg tcgtcgtgtc gccatcggga cggctgcgtt tttacgaaac
    12901 gacgtcgggg tgtccgacca cagcctgacg cagttggacg atgaagtggc ctcgcttcgt
    12961 cgtgaagggc agacgatcct gtttgtcgtc atcgatgagc aggttgccgg tgtacttggg
    13021 gtggcagatc ccatcaagga ctcgactcgt gacgcggtgc aggagctcaa ggccgaaggt
    13081 attcgtgtgg tcatggtgac gggagatcat cgcgatacgg ccgaagcagt cgccagacaa
    13141 ctcggcattg acgaggtcat ggccggtgtc ttgccggagc agaagggaca gatcattgcc
    13201 cgattcaagt cggaaggccg cgtggtcgcg atggccggcg acgg
//
LOCUS       DATIPF010000410         3584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_697342, whole genome shotgun sequence.
ACCESSION   DATIPF010000410 DATIPF010000000
VERSION     DATIPF010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697342"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2292
                     /gene="alaS"
                     /locus_tag="VLL06_10250"
     CDS             <1..2292
                     /gene="alaS"
                     /locus_tag="VLL06_10250"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HSS31388.1"
                     /translation="GLAKDRMWVTIFRDDDEADQLWKKVGVPDSRIIRCDEKDNFWQM
                     GDTGPCGPCSELHYDQGPSVPGDDRPNGAGDRVIEIWNLVFMQFNRDAAGKLHSLPKP
                     SIDTGMGLERLAAVAQGVYSNYDSDVFTPLLSVVATRAGVRYGEQETTDRSMRVVADH
                     LRAITFLMADGVLPSNEGRGYVLRRILRRAARHGRLLDIIEPFLYELTSAVVNQMGEA
                     YSEIRAAAATIAEATRGEEERFIATLDQGLPILNDMIAKARAAGQKVLSGADVFKLYD
                     TYGFPMDLIGEACREQGMTLDEAGFDRAIEEQRNRARKTGGFEQETARPAVAELTGRF
                     GATKFIGYDRLESESLVQAILKGDRLVTEAAEGDEVELVLDVTPFYAEGGGQVGDQGV
                     LMGQEGRVEIKETTRPVPTLILHKGTVTKGRIREGEALHLTVNATTRQDAQRNHTATH
                     LVHAALRDMLGPHVKQYGSLVAPNRLRFDFAHFRPLSSRDIDDIETVVNDEVRKNERV
                     ATEVMSIQDAVAKGALAFFGDKYGEQVRVVTVESFSKELCGGTHCQRTGDIGLFRIVS
                     ETGVAAGVRRLEAQTGSGALVHMKKLETDIRDLSDLLKVGQSELVAKTRKVMTQLKDK
                     ERELEELKLKMASGSAVESTAKTIAGIQVHVQRADGLDVNGMRALADQLRDKLKSGVV
                     ALGAATGDGKVSLLVVVTKDLIAKLKAGDIIKDMASEVGGTGGGRPEMAQAGGKDPAK
                     LDAALQKVFGLVERMLERYTHGG"
     gene            2282..2854
                     /gene="ruvX"
                     /locus_tag="VLL06_10255"
     CDS             2282..2854
                     /gene="ruvX"
                     /locus_tag="VLL06_10255"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HSS31389.1"
                     /translation="MEGKRILALDYGTKRIGVALSDELGWTAQPLETLNRRTLERDIT
                     HIASLVVTHEVGQVLLGFPLQLDGREGPAIQAMREFQARLEEGVTVPVILWDERLTTK
                     AAEDLLIAADVSRKKRKGVVDRIAASILLQSYLASLEPVLKKEPESWADGYEDRGTIE
                     PPHETTDHSRMSAGRGRRTRRRRISGDSLG"
     gene            2769..>3584
                     /gene="mltG"
                     /locus_tag="VLL06_10260"
     CDS             2769..>3584
                     /gene="mltG"
                     /locus_tag="VLL06_10260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HSS31390.1"
                     /translation="MKLRIILGCLLVAVVALGAAAYLAIRWAEGPVVPPEAHPPSKVV
                     IIPDGSTFQHVATLLEREQLIKSSVAFVLFGKSQSADRKVHAGEYELNPSLTPVEILS
                     KLITGQVVLHSLTIPEGLTITQIAEVASQQGLTDPGEFLRLAKDREFIASLGIKAETL
                     EGYLYPNTYKFPRPIKAREVLVAMVDQLKQELGPDLLARMQELKMTMHEVLTLASVIE
                     KETGSGGERPEISAVFHNRLKKGIPLQSDPTVIYGLPAFDGNLHKKDLSSPSPY"
BASE COUNT          793 a          888 c         1179 g          724 t
ORIGIN      
        1 ggtttggcga aggatcggat gtgggtgacg attttccgtg acgacgatga agcggatcag
       61 ctgtggaaga aggtcggagt cccggactcg cgaattatcc ggtgcgacga gaaggacaac
      121 ttctggcaga tgggagatac aggtccctgt gggccctgct cggaactcca ttacgatcaa
      181 ggaccgtcgg taccgggcga cgatcggccg aacggcgcgg gtgatcgcgt cattgagatc
      241 tggaatctcg ttttcatgca gttcaatcgt gatgctgctg gaaaactgca ttccttaccc
      301 aagcccagca ttgatacggg catgggtttg gaacgactgg cggccgtggc acaaggcgtc
      361 tacagcaact atgacagcga tgtgtttacg ccgttgctca gcgtcgtggc gaccagggct
      421 ggagttcggt atggggagca ggagacaacc gatcggtcga tgcgcgtggt ggcggatcat
      481 cttcgcgcga tcacattctt gatggcggat ggggtgttgc cgtcgaacga ggggcgcggc
      541 tatgtgctgc gccggattct gcgccgggcc gcacgtcacg gacggttgct ggacatcatt
      601 gaacccttcc tctatgagtt gacgtcggcg gttgtgaacc aaatgggcga ggcctattca
      661 gagatccgag ctgctgcagc gacgattgcc gaggcgacga ggggcgagga ggaacggttc
      721 atcgcgacac tcgatcaagg cttgccgatc ctcaatgaca tgattgcgaa ggcgcgcgct
      781 gccgggcaga aggtcttaag tggtgcggat gtgtttaagc tctatgacac gtatgggttt
      841 ccgatggatc tcattggtga ggcctgccgt gagcagggga tgacgctcga tgaggcggga
      901 ttcgaccggg ccattgagga gcagagaaat cgtgcacgca agaccggcgg gtttgaacag
      961 gagacggccc ggcctgcggt ggcggaactc acgggacgtt tcggggcaac gaagtttatc
     1021 ggctacgatc gcctggagag cgagagcctc gtgcaggcga ttttgaaagg cgaccggttg
     1081 gtcacagaag cggcggaggg cgatgaggtt gaattagtcc tggatgtgac gccgttttat
     1141 gcggaaggcg gtggacaggt cggtgaccag ggtgtactga tgggccagga ggggcgggtc
     1201 gagatcaagg aaactacgag accggtgcca accttgattc tgcataaggg aactgtcacg
     1261 aaagggcgga ttcgagaggg agaggcgttg catctgaccg tcaatgctac gacgcggcag
     1321 gatgctcagc gaaaccacac tgccacccat ttggtccacg ctgcgttgcg ggatatgttg
     1381 ggccctcacg taaaacagta cgggtcgctc gttgcgccca atcgcttgcg attcgacttt
     1441 gcccatttca ggccgctgtc gtcccgcgat atcgatgaca ttgaaacggt cgtgaacgac
     1501 gaggtgcgga agaacgaacg ggttgcgacc gaggtgatga gcattcagga tgcggtggcc
     1561 aagggggcgt tggcattttt tggcgacaaa tatggcgagc aggtgcgggt ggtgactgtc
     1621 gagtctttta gcaaggaact ctgtggcgga acacattgtc aacggacggg agacatcggt
     1681 ctgttcagga ttgtctcgga aaccggcgtg gcggctggtg tacgccgtct cgaagcccag
     1741 accggcagcg gggcgctcgt tcacatgaag aagctggaaa cggatattcg ggacctctca
     1801 gatctgctca aggtcggcca gtcagagttg gtcgccaaaa cacgcaaggt catgacgcaa
     1861 ctcaaagaca aagaacgcga actggaagag ctgaaattga aaatggccag cggatcagcg
     1921 gtcgagtcca cggccaaaac gattgccggt atccaggtgc atgtacagcg ggccgatggt
     1981 ctggacgtga acggcatgcg ggcgttagcc gatcagctgc gggacaagtt gaagagtggt
     2041 gtcgtggctc tcggcgccgc gaccggagac gggaaagtgt ctctactcgt tgtcgtcaca
     2101 aaggatttga ttgccaaact gaaagcgggt gacatcatta aagatatggc ctcggaggtc
     2161 ggtggtacag ggggcggcag acccgagatg gcgcaagcag gcgggaagga tccggcaaag
     2221 ttggatgcgg cgttgcaaaa agtctttgga ctggtcgaac gcatgttaga gcggtacact
     2281 catggagggt aaacgaattc tcgcgctcga ttacggcacc aagcggattg gagtggccct
     2341 gagcgatgaa ttggggtgga cggcccaacc acttgaaacg ctcaatcggc gcacgttaga
     2401 gcgggatatt acccatattg catcgctcgt tgtgacgcat gaggtcgggc aagtcctgtt
     2461 gggctttccc cttcaattgg atggccgcga gggaccggca atccaggcta tgcgcgagtt
     2521 ccaggcgcgg ttggaagaag gtgtgaccgt tcccgttatt ctgtgggacg aacggttgac
     2581 gacgaaggca gcggaagacc tcttgatcgc ggccgatgtc agccggaaaa agcggaaggg
     2641 tgtggtggat cgcatcgccg cttcgattct gctccagagt tatctggcga gccttgaacc
     2701 ggtgttgaag aaggagccgg aatcatgggc ggatgggtac gaggacaggg gaacgataga
     2761 gccacctcat gaaactacgg atcattctcg gatgtctgct ggtcgcggtc gtcgcactcg
     2821 gcgccgccgc atatctggcg attcgctggg ctgaaggccc ggtcgttccc ccggaagcgc
     2881 accctccctc aaaagtcgtc atcatccctg atggctcgac ctttcaacat gtcgcgactc
     2941 tgcttgagcg cgaacaattg atcaagagca gcgtggcctt tgtcctattt ggaaaatccc
     3001 aatcagccga ccggaaagtt catgccggtg agtatgaact gaatcccagt ctgactccgg
     3061 tggagatact ttcgaagctg atcaccggcc aagtggtgct ccactcgttg acgattcccg
     3121 aaggcctgac catcacccaa attgccgagg tcgcctcgca acagggcctc acagatcccg
     3181 gggagtttct gcggcttgcg aaagatcggg aattcatcgc atcacttggg atcaaggcgg
     3241 agacgctgga agggtatctc tatcccaaca catataaatt tcctcgcccc atcaaggcgc
     3301 gagaggtgtt ggtggcgatg gtcgatcagc tcaagcagga actcgggccg gatctcctcg
     3361 ctcgaatgca ggaactcaag atgacgatgc acgaagtgct gacccttgcc tccgtcattg
     3421 aaaaggagac cgggtcgggc ggcgagcggc cggagatttc cgcagtgttt cacaatcggc
     3481 tcaaaaaggg tattccattg cagagcgatc ccaccgtgat ttatggcctg ccggcattcg
     3541 atggaaattt gcacaagaaa gacctgtcca gccccagtcc ctac
//
LOCUS       DATIPF010000411         5661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1492282, whole genome shotgun sequence.
ACCESSION   DATIPF010000411 DATIPF010000000
VERSION     DATIPF010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492282"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VLL06_10265"
     CDS             <1..373
                     /locus_tag="VLL06_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HSS31391.1"
                     /translation="ILRDIGTDAAQGRIYLPQEDLEKFGCTDQVLLQRQESTELRTLL
                     HFEAARAHEYYAKAQAAFEALPAQDQRSLTVSEIMRAVYVRILQKLERPKHRIFGPRA
                     RLATTHRLAVAAGVWIRSRSS"
     gene            397..1695
                     /locus_tag="VLL06_10270"
     CDS             397..1695
                     /locus_tag="VLL06_10270"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSS31392.1"
                     /translation="MIIGGGPAGLAAAYRLSRQGNRVTLMDRRPLLGGTAISEDPRES
                     AEPFTILGCHRATHALLHALHPGPQQPSEAEIPLEFHLPDDSLVHYPRTYFPAPLHTW
                     MNLLKFSGIPWKERWRLASWMEKLWEGDERLPADLEQRTADEWLASIKQTTRTRRVVW
                     NPLARWLTGNNLSTMSADAFVTSMKPLFLNTRLDSRISIVQDSLQTCVVQPVTEALTQ
                     VGATILRNTEATQLRYAQDRISGVLLQDGSTLHADWYVAALPPQLLTPLLPERWITRY
                     AYFQQLTELRSVDSTTLHVDAEQPCPIPRLVLLNDTSFHSVLATAATPDRTRFSLITT
                     DSQFAQVRSDSHHDTVIPDLLRSLGLLKPESRIASFHRRTIPNAILSLRPGTKIHRPI
                     QRSPIANLLLAGSWTDTGWPPNLESAIVSGNHCADAIAVR"
     gene            complement(1827..2483)
                     /locus_tag="VLL06_10275"
     CDS             complement(1827..2483)
                     /locus_tag="VLL06_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31393.1"
                     /translation="MPFMKRLVFGFLVLLMSVGVVWAGERGYQLAAGTESKLCARVLD
                     AFREDVDDRGRLRYQHEIFRQITWKPVELKGQAPKTRHCASLDQAIFDVDHSGQPDLV
                     VKTTFCMQGSPSDSLYIFSADSAVLERSNWQDLSPLLATPNKFERTGGTYPMTQLQAE
                     EPGVGRAPLTGVFTVQSFMLDGSAYFSLTDRRAEWIVIAQYRGGTQFEDLCYLRTSTQ
                     "
     gene            2617..3264
                     /locus_tag="VLL06_10280"
     CDS             2617..3264
                     /locus_tag="VLL06_10280"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HSS31394.1"
                     /translation="MSLLELSKSLHNCQRCKLAKLGRSQVVFGVGNPHATVMFVGEAP
                     GFHEDQQGEPFVGAAGKLLNDLLQSAGLSRSDIYIANVIKCRPPNNRDPEPDEVETCK
                     PFLMQQIAMIHPKLVCTLGNWATQTLLERKVGITKVRGQAFYLKDFVLFPLLHPAAAL
                     HQGNMLEPLREDFKKLREFLDRNTKPAEPTTTTSVAAPTLHIEPPQPTQMDLFGS"
     gene            complement(3617..4072)
                     /locus_tag="VLL06_10285"
     CDS             complement(3617..4072)
                     /locus_tag="VLL06_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31395.1"
                     /translation="MPLNAELGKIMLQLITSRYDDRHWRKKIEKTLGLPQSGVGDPVQ
                     QQIFMYLKIGLKGYKSRRADPDSWIVGGYATKEIVERAKFQTQLVGPDITKDDVGFLG
                     SDPGKEIDETWWDEMLVSWFDVPEEEKPAEEEGGEAADSDPSSTIKAKV"
     gene            complement(4089..4796)
                     /locus_tag="VLL06_10290"
     CDS             complement(4089..4796)
                     /locus_tag="VLL06_10290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSS31396.1"
                     /translation="MAESIREIRVQYPSDKVTMRAYVAAPQTKEPRPAIIVIQEWWGL
                     SEHIMDVTRRFAGEGYVAIAPDLYSRLGHVLTTDASEAGKLMNTLQQDDGLKDLNATV
                     TYLKSVPEVDSTRIGVTGFCMGGSYALMLPCVNRDVKAAVPFYGQVPNPDAPLQQLSA
                     PVLYIYGEDDGWITKADVQRLAAGFKKYNKSGEIKTYPGAPHAFFRDSDMTVYRPEAA
                     KDAWTRATAFFKQHLRP"
     gene            complement(4840..>5661)
                     /locus_tag="VLL06_10295"
     CDS             complement(4840..>5661)
                     /locus_tag="VLL06_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HSS31397.1"
                     /translation="MSVSPVATDPESGAMQRDSWYAVQRKFAKLDTPEVVGLEAARRT
                     IRKLGARKVATQRVPVVFDPETAGSLMGNLCSAVSGYSLYKGASFLAGQLDKPLAPEY
                     VTVYDDGRVVGGLGSRPFDGEGLPTRKTTVVERGVLKSYLLDTYSGRKLGLASTGNAS
                     RSVGENPSVGPTNFYLAPGTKTAQEIIQTMKQGLYVTDLIGFGINMVTGDYSRGAAGF
                     WIEGGELAYPVEEITIAGNLKDMFAGIEMIGSDLVFRGRIASPTVKLAEMMVAGS"
BASE COUNT         1227 a         1771 c         1417 g         1246 t
ORIGIN      
        1 cattctacgg gacatcggaa cagacgcggc tcaaggtcgc atctatctcc cgcaagagga
       61 tctcgaaaaa ttcggctgca ctgatcaggt cctcctccaa cggcaggaga gcacggagct
      121 ccgcacgttg cttcactttg aagccgcaag ggcacacgag tactatgcaa aggcacaagc
      181 ggcgttcgag gccctcccgg cacaggacca gcggtcgctg actgtctcag aaatcatgcg
      241 tgcggtgtat gtccgtattc tgcaaaaact cgaacggccg aaacatcgga tatttggccc
      301 ccgggccagg ctcgccacga ctcatcgatt agccgtggcc gccggagtct ggatccgctc
      361 ccgatcctcg tgacgtccat cattgatcag tccgttgtga taattggtgg aggcccggcc
      421 ggccttgccg ctgcctaccg cctcagccga caaggcaatc gagtgacact catggatcgt
      481 cgccccctgc ttggaggcac cgccatcagt gaagaccctc gtgagagcgc tgagccattc
      541 accatcctcg gatgccatcg cgcaacacac gccctactgc atgcgctgca tcccggccca
      601 cagcagccgt cagaggccga gatcccgtta gaatttcatc tccctgacga ctccctcgtg
      661 cattaccccc gtacctattt ccccgctcct cttcatacat ggatgaacct cctgaaattt
      721 tccggtatcc cctggaaaga gcgctggcgg ctggcatcat ggatggagaa gctgtgggag
      781 ggcgacgaac ggttgcctgc cgatctggag caacgcaccg ccgatgaatg gcttgcgtca
      841 atcaaacaaa ccacgcggac gcgccgcgtc gtgtggaatc ccctcgcgcg atggctgacc
      901 ggcaacaatc tctccacgat gtccgccgat gccttcgtca catcgatgaa gccgttgttc
      961 ctgaacactc gtttggacag ccgtatttcg attgttcagg actcgcttca gacctgtgtc
     1021 gtacaaccgg ttaccgaagc actgacccag gttggcgcga ccattttgcg caatactgaa
     1081 gccacacagt tgcgatatgc acaggatcga atctcgggcg tcttgctgca ggacggttcc
     1141 actctgcatg ctgattggta tgtcgccgcc ttgccccccc aactgctcac gcccttactt
     1201 ccagaacgat ggataacccg ctacgcctat tttcaacagc tcacggagct gcgatcggtc
     1261 gacagcacca ccctccatgt ggatgccgag caaccctgtc ccatacctcg cctcgtcctg
     1321 ctgaatgaca cgtcattcca ctcggtactc gccacagcgg ccacacccga tcgcacacgt
     1381 ttctctctta tcacgaccga cagtcagttc gcgcaggtcc gatcggactc acatcatgat
     1441 accgtgatcc cagatttgct cagatcattg gggctcctca aacctgaaag caggatcgca
     1501 tcattccaca ggcgaacgat tcccaatgcc atcctctcac tcaggcctgg cacaaaaata
     1561 catcggccca tccaacgcag tccgattgcc aacctactgc tggctggtag ttggaccgat
     1621 acaggatggc ctcccaacct cgaaagcgcc attgtcagtg gcaaccattg tgccgatgcg
     1681 atcgcggttc gctagatcca ctcgaccatg accagatcgg atcgtcatat cgtgatgggt
     1741 tcgcgtccat atggactatc gaatatgaac atggtacggc gagatatgtg tccggctacc
     1801 aaagaaggga gcgtcgagga ggaaacctac tgcgttgacg tacgaagata acaaaggtcc
     1861 tcgaattgtg tgccgcctcg gtattgcgca atgacgatcc attcagcacg tctgtccgtc
     1921 aaactgaagt atgcgctccc gtccagcatg aaggattgga ctgtaaagac accggtcaat
     1981 ggcgcccggc ccacacctgg ttcctctgcc tgaagttgtg tcatgggata ggtcccgccc
     2041 gtccgctcga acttattggg ggtcgccagc agaggggaca ggtcctgcca attcgatcgt
     2101 tcgagcaccg cactgtcggc agaaaaaata tagagactgt cgctcgggct cccctgcata
     2161 cagaacgtcg tcttgaccac caaatccggt tgcccactgt gatccacgtc aaagatcgcc
     2221 tgatcgagac tcgcacagtg cctcgtcttc ggcgcttgtc ctttcagttc aaccggcttc
     2281 caggtgatct gacgaaagat ttcgtgctgg tatcgtagcc gccctcgatc gtccacatcc
     2341 tcacgaaacg cgtccagcac tcttgcgcaa agcttgctct ccgttcctgc ggcaagctga
     2401 taccctcgct cgccggccca gaccacaccg acactcatca gtaagacgag aaaaccgaac
     2461 acgagccgtt tcatgaatgg catatctttc ctcctcaacc ccttctgcac ttggcaattt
     2521 taagcaacta ttgttcccac gtttctcttc cattgacaag aaaagtcggc ccgcctaaaa
     2581 tccttcaaca tttttcaact gctccccttc actatcatgt ccctcctgga actcagcaag
     2641 tcactgcaca actgccagcg ctgcaaactg gccaagctgg ggcgcagcca agtcgtgttt
     2701 ggcgtgggga atccgcatgc gactgtgatg tttgtaggag aagcgcctgg tttccatgaa
     2761 gaccagcaag gggaaccgtt tgtcggagcg gcaggaaaac tcttgaatga tctcctgcaa
     2821 tcggcggggt tgtcccggag cgacatctac atcgccaatg taatcaaatg ccgtcccccg
     2881 aacaaccgcg acccggaacc ggatgaagtc gagacgtgca aaccgtttct gatgcaacag
     2941 atcgcaatga tccacccgaa actcgtctgc acgttgggga attgggcgac gcaaacatta
     3001 ctggaacgga aggtagggat taccaaggtc cgcggccagg cgttctactt gaaagatttt
     3061 gtcctcttcc ccttgctcca tcccgccgca gccctccacc aaggcaacat gcttgaaccg
     3121 ctgcgtgagg actttaaaaa actgcgagag tttctggatc gaaatacgaa accagccgaa
     3181 cccacgacaa cgacatcggt tgctgccccc acactacaca ttgaaccgcc gcaaccgacc
     3241 cagatggacc tcttcgggtc ataactcgag cgcttctctc ctctcctcac ctttatttga
     3301 tgccgcctgt cagagagtgt ttcctctcag atgagattgg cctgagatct caaagaatgg
     3361 ctaacaaatg gcttgaaccg attccggctc agccttcccc cagcggcgcc cgctgctgcc
     3421 gccgcgtgca tcagcgcggt gctgagtctc gccgcagtga agtcatgcgg cccgctctca
     3481 gcgggtggca cagactgttc gacgctccgt tcgaccgaag gtctgaacgg agctagtttc
     3541 tgtgccaagt gtggctgagc atgagggcga gccagcaggg cgctggcaag agcggaagca
     3601 gcagcgggcg gcggtcctag actttcgctt tgatggtgga cgaggggtct gaatcggcgg
     3661 cctcgccgcc ttcctcctcg gcaggctttt cttcttccgg cacatcgaac caggacacaa
     3721 gcatttcgtc ccaccaggtt tcgtcgatct ctttcccggg gtcgctaccg agaaacccca
     3781 catcgtcctt cgtgatatcg gggccgacta actgggtctg gaacttcgct cgttcgacaa
     3841 tttctttcgt ggcataccct ccgactatcc aggagtccgg atcggcccga cgagacttgt
     3901 agcccttgag tccgatcttg agatacataa agatctgttg ttgcacagga tccccgacac
     3961 cagattgcgg caaacccaag gtcttttcaa tcttcttacg ccaatgccgg tcgtcgtatc
     4021 gcgacgtaat caactggagc atgatctttc ccaattcggc gttaagcggc atggtacgtg
     4081 tgatccctct acggtcttag atgctgttta aaaaatgctg tggcccttgt ccaggcgtct
     4141 ttggctgcct ccggccgata tacggtcata tccgaatccc gaaaaaaggc atgcggcgcg
     4201 ccaggatagg ttttgatttc gccggattta ttatatttct tgaaccctgc cgcgagccgt
     4261 tggacatccg ctttggtgat ccaaccatca tcctctccat aaatgtacag cacaggggct
     4321 gaaagttgtt ggagcggtgc atccggattc ggcacctgac cgtagaacgg cacggctgcc
     4381 ttgacgtctc tattcacaca gggcagcatg agcgcatagg atcctcccat acagaagccg
     4441 gtcaccccga ttctggtcga atccacttca ggcacggatt tgagataggt gaccgtcgca
     4501 ttcaaatctt tgaggccatc gtcctgttgc agggtgttca tgagcttgcc cgcttcgctg
     4561 gcatcggtcg tgagcacgtg cccaagccgc gaatacagat ccggcgcgat ggccacatac
     4621 ccttcaccgg caaagcgtcg tgtcacatcc atgatgtgtt cgctcagtcc ccaccattcc
     4681 tgaatgacaa tgatcgcggg tcgtggctct ttagtctgcg gtgctgccac ataggcccgc
     4741 atcgtcacct tgtcgctagg atactggact cgtatttctc tgattgactc ggccatgggt
     4801 cacctcgctg atacgggcgt agtgatacgc catctgctct caggagcccg ccaccatcat
     4861 ctctgcaagc ttcaccgtcg gactggcaat gcgaccacga aacacaagat cactgccgat
     4921 catctcaatc cccgcaaaca tgtctttgag attgcccgcg atcgtgattt cttccacggg
     4981 ataggccaac tcgccacctt cgatccaaaa cccggcagcc ccccgtgaat aatcaccggt
     5041 caccatgttg atgccgaaac cgataaggtc cgtgacatac agcccttgtt tcatcgtctg
     5101 aataatctct tgggccgtct tcgtccctgg cgcaagatag aagtttgtgg gaccgaccga
     5161 gggattttca ccgacacttc gcgaggcatt gccggtcgac gccaacccaa gtttccttcc
     5221 agaataggta tcgagcaggt agctcttcaa cacccctcgt tccaccactg tcgtcttcct
     5281 cgtcggcaaa ccctccccat cgaacgggcg agagcccagc cccccgacca ctcgtccatc
     5341 gtcgtacacc gtgacatact caggggccaa cggtttgtcc aattgaccgg ccaaaaacga
     5401 agctccttta tagagtgaat agccggacac cgcgctgcag agatttccca tgagacttcc
     5461 agccgtttcg ggatcaaaca cgacaggaac tcgttgggtg gccactttcc tcgctccgag
     5521 ctttcggatc gtccgccgtg ccgcttccaa tccaacgacc tccggggtat ccagcttcgc
     5581 aaacttccgc tgcaccgcat accaggagtc ccgttgcatg gccccgctct ctggatcggt
     5641 cgcgacaggg gacaccgaca t
//
LOCUS       DATIPF010000412         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_252117, whole genome shotgun sequence.
ACCESSION   DATIPF010000412 DATIPF010000000
VERSION     DATIPF010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252117"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="VLL06_10300"
     CDS             complement(<1..1331)
                     /locus_tag="VLL06_10300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSS31398.1"
                     /translation="MNQQGYRKNFMVGVVAIGVIAGAAGGFITAHGMMETGSVSAAPV
                     LAGEMADMGAGGVSTPPGHGENGLSEQAGGGRVKTSGEMGGMEGMSMEQMDMKKMAPA
                     GEKSMEGMPGMSSAPSGAVVVPAVMRQLIGVRSAPAGIAVLGQEIRAVGTVGYDERGF
                     TQVTVKTAGWVREVFINSIGRPVRKGEPLFTVYSPDLLATQDEYLLAVKMGSQLESSP
                     FAEVKANAASLITSTRERLRLWDVTDTQMATLERRGKAESVLTVYAPSSGIIMKREAV
                     PGKYVEPGTTLYEVADLSTVWVSADIYESEVAAVTLNQQASVAFAAYPGETFQGTISY
                     VYPTLNTEARTVRVRVELPNPGLKLKPGMCGTVILQTESTKRLVVPKEAVLDTGLRHL
                     VFLDRGEGAYAPYPVKLGRRNQDSVEVLEGIKEGDRIVTSANFLLDAESKLT"
     gene            complement(1354..>2200)
                     /locus_tag="VLL06_10305"
     CDS             complement(1354..>2200)
                     /locus_tag="VLL06_10305"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSS31399.1"
                     /translation="FLAQKAVQIHHEQVEVLRQFVEVANAKFRAGKGSQADVLKAHVE
                     LSLLFQHRPVLEQRRETAEAMLNTLLDRDPASPLGMVQEPSQLPLDQTIEDLHRLALN
                     DRPEIKAAELDVQRNEQSHALALRQYYPDFNVAFQRFQNYQANDGFGAYVAMSIPFAF
                     WTKPKYDAGVQEAAAGVAVARAQQHTIENITRFQINDLLAKLRAIDQVATLYRTTILP
                     QGEQTLEAARVGYRSGKAGVLDLIEAQRAWQGFQLEYFKALVDRQQRLAELEQVVGIT
                     LDRQS"
BASE COUNT          433 a          707 c          565 g          495 t
ORIGIN      
        1 gtcaacttac tctccgcatc caacaaaaaa ttggccgagg tcacgatccg atccccttcc
       61 ttgattcctt ctaacacttc aacggaatcc tggttcctgc ggccaagctt caccgggtac
      121 ggcgcatacg cgccttcgcc acgatccagg aatacgaggt ggcgaagtcc tgtgtccagc
      181 acggcttcct tgggaaccac caatctcttc gtcgactcgg tctgcaagat gaccgttcca
      241 cacatgcccg gcttgagttt gagtccagga ttcggcaatt ccacccgtac ccgcaccgtc
      301 cgagcctcgg tattcagcgt ggggtataca tatgagatcg tgccctgaaa cgtttctccg
      361 ggataggcag cgaaggcgac ggacgcctgc tggttgagtg tcacggctgc gacttcagac
      421 tcatagatat cggcagaaac ccagaccgtg gagagatccg ccacttcata caatgtcgtg
      481 cctggttcca catattttcc tggcacagcc tcccgcttca tgatgatgcc ggaggacggg
      541 gcatagaccg taagcaccga ctccgcctta cctcgacgtt cgagcgtggc catttgtgta
      601 tcggtcacgt cccataatcg caggcgttcc cgcgtactgg tgatgaggga tgccgcgttg
      661 gctttcactt ccgcaaaggg actgctctcc agttggcttc ccatttttac ggcgaggaga
      721 tactcatcct gggtggccag aaggtccggc gaatagaccg tgaagagtgg ttcacctttg
      781 cggactggtc ggccgatcga attgataaag acttctcgca cccacccagc cgtcttcacg
      841 gtgacttggg taaatccccg ttcgtcatag ccgactgtgc cgaccgcacg gatctcttgc
      901 cctaggactg cgatgccggc cggcgcgctt cgaaccccga tcaactgcct catgaccgca
      961 ggaaccacca cagcccctga cggcgcagaa gacatgcctg gcattccttc catagacttt
     1021 tcgcctgccg gagccatctt cttcatgtcc atctgctcca tggacatgcc ctccatccct
     1081 cccatctcac ccgatgtttt caccctccca cccccggcct gctcagacag gccgttttcc
     1141 ccgtggcctg gaggcgtcga gacgcctcct gctcccatgt cagccatctc tcctgccagc
     1201 actggggcag ccgagacgct ccctgtttcc atcatcccgt gggcggtaat aaaaccccca
     1261 gcggcgccgg cgatcacacc gatcgcgacc acaccaacca tgaaattttt tctgtatccc
     1321 tgctggttca tatgttcctc cctgttcatg atgttagctc tggcgatcga gagtaatgcc
     1381 gacgacctgc tcaagttcag ccaatcgctg ctggcgatcc acgagggctt tgaaatattc
     1441 aagttgaaat ccttgccagg cccgttgcgc ttcaatgaga tcgagaaccc ctgctttgcc
     1501 ggaccgatac cccacacgcg ccgcctccaa agtctgctct ccctgtggca agatcgtggt
     1561 gcggtacaac gtggccacct gatcgatcgc tctgagcttg gccaggaggt cattgatctg
     1621 aaaccgagtg atgttttcta tggtatgttg ctgcgcccgc gcgaccgcaa cccccgccgc
     1681 tgcctcctgc acacccgcgt catacttcgg cttagtccag aacgcaaacg ggatactcat
     1741 cgccacgtag gcgccgaatc cgtcgttggc ctgatagttc tggaagcgct ggaacgccac
     1801 gttgaagtcc gggtaatact gacgcagagc cagcgcgtgg gactgctcgt tccgctgtac
     1861 atccagctcg gcagctttga tctccggcct gtcgttcagc gccagacggt gcagatcctc
     1921 tatggtctga tcgaggggaa gttgagaggg ttcttgcacc atgcccaatg gtgatgcggg
     1981 atcccgatcg agcagcgtat tcagcatcgc ctccgccgtt tcccggcgct gttccaagac
     2041 aggacgatgt tggaacaaga gagataactc cacatgggcc ttgaggacgt cagcctggct
     2101 tcccttgccc gcacggaact ttgcgttcgc aacctctaca aactgtctga gcacctccac
     2161 ctgttcatga tgaatctgga cggccttctg cgcgagaaac
//
LOCUS       DATIPF010000413         6427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1755884, whole genome shotgun sequence.
ACCESSION   DATIPF010000413 DATIPF010000000
VERSION     DATIPF010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755884"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VLL06_10310"
     CDS             complement(<1..779)
                     /locus_tag="VLL06_10310"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS31400.1"
                     /translation="MARMSAGELYERGRVLKHANMYDEAVDDFQHAACDPQYAGKAQA
                     QLALCFRAMGRKEEAVEAFRLALESSTFSSNENLHLLYLLGETLESLGHTAEALEAYG
                     WVRQEDPGFRDVVPRIKHLCAGGRGPVTQSLLVRQFRVGDLFTIYGQLRRRSLSLLEQ
                     TRQALTQSTGSHRIARSTPREVTSAQRTNPGVSASQPMMGRQSSMVMRQHVRVAIQGR
                     SQFSSKSQNMTGEGQLRDLSPGGCRVTSSVPVPVGAELVCW"
     gene            1267..1440
                     /locus_tag="VLL06_10315"
     CDS             1267..1440
                     /locus_tag="VLL06_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31401.1"
                     /translation="MKMFQVRQSIHYELTVSAGTEQEAILKVGGIPLTQWDLDSTEIT
                     AELLDETEEIDDY"
     gene            2434..5376
                     /locus_tag="VLL06_10320"
     CDS             2434..5376
                     /locus_tag="VLL06_10320"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HSS31402.1"
                     /translation="MRWLLRPSGLAILALSLVCLYALAGFVLVPYLITAYAIPAISEK
                     LKRPVLVKAVELNPFVLSLRLTGFEIRESDQSTLLGFDEFFVNLQASSLIRRAYVFDA
                     IRLTMPYVSARISKDGRMNLAELVPRDDAPQPIAPQQTEKDKAGIPAIEIGEFEIGQG
                     VVEFRDESRPKPYMLDIVPIHIVLKNFHTKPGGDNLYVFTAELGKGETLSWAGKVSLE
                     PIRSSGTFSLSGVKLSTLWQYLHDRFRFDVSDGTIAADASYSLDAGVTPMKLQVSNAN
                     VQIEQLAVKEDGGLDPVIMIPELNVKGVDIDLVTHEVTVRNIAVDRASFTAWLNPDGT
                     VNYQQMFAPVDSVQPPTAADSATPPSKEEKPWAVWLKEITLKEHAIDFEDRTLPTPVH
                     VEVRALTVKSRDVRIPIKQALPIEVGMQLNETGLIHMDGSILPNPFQADVTLMLKDIA
                     IRPFQPYFEKFARIDVQSGAVNLNGAVHLATDHPNGPLMSYEGNGGVAALSVADRDQG
                     DEVASLHSLSLNGVRVTVDPTTVSIHEVGLQQPLAHLVVLSDGGSNLARLAVPPPFSA
                     SKDVKPEPQKAKGPAVPVTVGTVNLTKAALTFRDESVQPPVQTGLSALTGTIKGLSSK
                     QLARADVDLTGRVGKAAPLKIFGTINPLSENAFTDLIITLGGLDLAVGGPYSGKYVGY
                     GLSKGKLSLDLKYKVSQKQLEAENKVSVDQLTFGRKVDSPDATSLPVPLVVALLKDRK
                     GRIDIDLPIRGDLNDPDFKYGKAVISTLLNLLTKIVASPFSLMGKLIPDGGDEESLQF
                     IAFPPGSTSVAKEELKKLEALAKGLEERPNLRLDITGTADTVRDRQALNRMKLQAIVQ
                     AKWQREHVKPAAKEEPIPAGDEQRLIQQLYEAHQKNTIQSAAPLTVDPSAKPLTYEEQ
                     KHVLIAAMPLDEEALRGLALQRAEQVREQLTGEGKLADEQVNLLDVELNESDHDQIRS
                     RLAIEAAP"
     gene            5491..6126
                     /locus_tag="VLL06_10325"
     CDS             5491..6126
                     /locus_tag="VLL06_10325"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HSS31403.1"
                     /translation="MAVRLGDEAPNFTAETTEGTVNFHEWLGGGWGILFSHPKDYTPV
                     CTTELGTVAKITPEFKKRGVKVIAVSVDPLDSHKGWINDINETQKTTMNYPIIADPDK
                     KVATLYDMIHPNAIDNMTVRSVFIVGPDKKVKLTLTYPASCGRNFDELLRVVDSLQLT
                     SKFKVATPANWKDGEDCIITPAVNDAEAKTLFPKGFKAVKPYLRYTPQPNK"
     gene            complement(6261..>6427)
                     /locus_tag="VLL06_10330"
     CDS             complement(6261..>6427)
                     /locus_tag="VLL06_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HSS31404.1"
                     /translation="SGRVYAAYGGVYVATALVWLRVVDGEKLSVFDWTGAGIALFGMA
                     VIVFGWTPKE"
BASE COUNT         1471 a         1738 c         1765 g         1453 t
ORIGIN      
        1 atccaacaca cgagctccgc tccgactggg acggggacgg aactcgtgac acgacagccc
       61 ccgggcgaaa ggtctcggag ctgtccctca cccgtcatgt tctggctctt cgaggaaaat
      121 tggctgcggc cttggatggc cacacgcaca tgttgacgca tgaccatcga gctttgtcgc
      181 cccatcatgg gttgactagc tgagactcca ggattggtgc gttgcgctga ggtgacctct
      241 cgaggcgtac tcctggcaat ccggtgactt ccggtagact gagtgagcgc ttgccgggtc
      301 tgctcgagca aagaaagact acgacgcctg agctggccgt atattgtgaa caggtcccca
      361 actcggaact gacggaccaa gaggctttgg gtcacagggc ctcgtcctcc ggcgcacaga
      421 tgtttgatcc ggggcaccac gtcgcggaac ccggggtcct cttgtcgaac ccagccatac
      481 gcctccagcg cttccgcagt atggccaagc gattccaagg tctctcccaa taaatacagg
      541 aggtgcaggt tttcattgga tgagaaggtc gaagactcga gggctaggcg gaaggcctcg
      601 acggcctctt ccttgcggcc catggcgcgg aagcacagcg ccagctgcgc ctgagctttt
      661 ccggcatact gcgggtcaca ggctgcgtgt tgaaaatcgt cgaccgcctc gtcatacatg
      721 tttgcgtgct tcagcactcg cccgcgttca tacagttcgc ctgccgacat ccgtgccatt
      781 gcgggatcct cccgtttgtg cgctgtccag agccgatttg cgccatagag gagcaaaaat
      841 aggaccctgc ggcacaccca tgtcggaccc cgaaaatcaa acacttgtgc gcgctgggtg
      901 ggaggggagt caaccaggct gcccggaaag gtgcagctga taggttaggc ccctcacact
      961 ctggggcacg gcggtttaag atggggaagc cggaacctga gcgcagaaga cgaagccttg
     1021 aagaggggtt tccgatgatc tccggttaag gtggcatcgg ggtcagcgaa tacgcctccc
     1081 tgcaatgcct ccatcttgcc agtagccggt gggacttgtt caatgctagg atccgccagt
     1141 cacgcaccag gggtggtgaa ccgatgtaag cgagcaggcc atccagcgaa tgtatcgctg
     1201 gtcgccgatt ctttcaacga cgtggagatg ttgtgatatt catggtcggc gtgaaaaggt
     1261 aactgtatga aaatgtttca ggtgcgacaa agcattcatt acgaattgac ggtcagtgca
     1321 ggcacggagc aggaagcgat tctgaaagtc ggaggtatcc ccttaacgca gtgggatctc
     1381 gatagtacag aaatcacggc cgagttactc gacgaaacgg aagaaataga cgattactga
     1441 agtccaaggc ctgtccgacg tccacgatat ggggcctcct cttaccggct ctggtctcct
     1501 ctgattctga accacccgag gcaccttggg cattcttctc tctctgatct tcgggatgag
     1561 agtctgcgtg tgctggggca aagaaaggat gatgaggtcg aaccacctct acggcacgag
     1621 gattttaggc agacattaca gcaggctata aaacgcgcaa atcagcttct atcatggcag
     1681 ggtatagagt gcaattacgt cggcgggtcc acagggccgg cccattctcc atctaatgta
     1741 ttgaccggta ctgagtccga gccctcaaat cgaacgtaca gttcttgttt atcccaccac
     1801 tcccagactc tgcagatcac ggcctcctta tcttggcgcc accaatacca gccttttttt
     1861 gttggcttct cgctcgtcca gttcatatcg gcggctccag cggtccggcc cactcgtatt
     1921 tctcgtaaca gtcagaatcc aatggaaagg aaaatgggtc gtcggcatcg accagctcgg
     1981 ctccaggcca gcgctcttct attcggagga tattatagga atacacagat ttattactct
     2041 cactcttcca ccttgtccag taccaccccg gcaccatagg tttcctggtt atccaagtca
     2101 acggcgtcat gtccatcccc cataaaccca ccagttattg gaggagtgtt tggcattgac
     2161 cgcgacattg tagcacgagc ggcgtaagtt catcgaggac tgtctggaag aataacgaga
     2221 ctcgcatggt ggtggttgcg tctcgatctg atttcccata ttcactattc gaactcctga
     2281 acacatcggg ccggtgcaca caatggtcct atagcccgtt gcgagggtcg tattctcacg
     2341 caatctcacg tatgcttatc ttgcccggat gtctctgctc taagcggaaa attgctggtt
     2401 cgatcccagc ctggtgcacg atagggtttc tccatgcgat ggctgctccg tccctccggt
     2461 cttgcgattc tggcattgag tttggtgtgt ctctatgccc ttgctggatt tgttctcgtt
     2521 ccctatctca ttacagccta cgcgatccct gctatttctg aaaagctgaa acgcccggtc
     2581 ctggtcaagg cagtcgagct gaatcccttt gtgttgtcgc ttcgattgac aggattcgag
     2641 attcgagaat cggatcagtc caccttgctg ggattcgatg agttcttcgt caatcttcag
     2701 gcgagctctc tcattcgtcg ggcctacgtg ttcgatgcga ttcgactgac aatgccctat
     2761 gtgtctgcac gaatttctaa agacggccgc atgaatctgg cggagctcgt gccgcgtgac
     2821 gatgcgcctc aaccgattgc gccgcagcag accgagaaag ataaagccgg gataccggcg
     2881 atcgagatcg gcgaattcga aatcgggcaa ggggttgtcg aatttcgcga tgaatcgaga
     2941 cctaagccgt acatgctcga tattgttccg atccatattg tgctcaagaa tttccatact
     3001 aagccgggcg gcgacaactt gtatgtcttc acggcagagt tgggcaaggg tgaaactctc
     3061 tcctgggccg gcaaggtctc gctcgaaccg attcgatcct ccggtacgtt ctctctctcc
     3121 ggcgtgaagt tgtccaccct ctggcaatac ctccatgacc gattccgctt cgatgtgagc
     3181 gatggcacga ttgcggccga cgccagctat tcgctggatg ccggtgtgac tcccatgaag
     3241 cttcaggttt cgaatgctaa cgttcagatt gagcagcttg ccgttaagga ggatggaggc
     3301 ctagatccgg tcatcatgat tccggaactt aatgtgaagg gtgtcgacat agatctcgtc
     3361 acgcatgagg tcacagtccg gaacattgca gttgaccgtg cgtcatttac agcctggtta
     3421 aatcccgatg gcactgtgaa ttatcagcag atgtttgccc ctgtcgattc cgtccagccc
     3481 ccgactgctg cggacagcgc cactcctcca tctaaagaag agaagccctg ggcggtctgg
     3541 ctcaaggaga ttaccctaaa ggagcatgcg atcgattttg aggatcgcac cttgccgact
     3601 ccggttcatg tcgaggtgag agccttgacc gtcaagtccc gcgacgtacg gatcccgatc
     3661 aaacaggccc tgcctattga ggtgggaatg caactgaatg aaaccggcct gattcacatg
     3721 gatggttcga tcctgcccaa cccattccaa gcagacgtca ccctgatgct caaggacatc
     3781 gcgatcagac cgtttcagcc gtactttgaa aaattcgcga ggatcgatgt ccagtccggc
     3841 gccgtcaatc taaatggagc ggtgcatctt gccaccgacc atcccaacgg tccactcatg
     3901 tcatacgaag gcaatggcgg cgtggcggca ttaagcgtgg ccgaccgtga tcagggtgat
     3961 gaggtggcct cgttacactc attgtccctc aacggtgttc gtgtgaccgt cgatccgact
     4021 accgtttcga ttcatgaggt gggccttcag cagccgttgg cgcatcttgt ggtgctttca
     4081 gatggcggat cgaatctcgc gaggcttgcc gttcccccgc cgttctctgc atcgaaggat
     4141 gtgaagccgg agcctcagaa agccaagggc ccagccgttc cagtaacggt tgggacggtg
     4201 aatctcacca aagcagccct gacgtttcgc gatgaatcgg tgcaacctcc tgtccaaaca
     4261 ggactttctg ctctaaccgg tacgatcaag ggattgtcgt cgaagcagct tgccagggcg
     4321 gacgttgacc tcaccgggcg agtgggtaag gcggcgccgc tcaagatttt cgggaccatc
     4381 aatcccttga gcgagaatgc gttcacggat ctgatcatca cccttggggg cttggatttg
     4441 gctgtgggag gtccctacag cggcaagtat gtgggctatg gattgtcgaa gggtaagctc
     4501 tcgctcgatt tgaaatacaa ggtctctcaa aaacagcttg aagcagaaaa caaagtgtcg
     4561 gtcgatcaat tgacattcgg gcggaaggtc gatagtccgg atgcgacgtc gctgccagtt
     4621 ccgttggttg tggccctctt gaaagatcgc aagggacgga tcgacatcga tttacccatt
     4681 cggggggatc tcaatgatcc cgacttcaag tacggcaagg cggtcatctc aacattgctc
     4741 aatctactga cgaaaattgt ggcgtctccg ttttcgctga tgggaaagct tattcctgac
     4801 ggtggagacg aagaaagttt acagttcatt gcgtttcctc cgggcagcac gtcggtagcc
     4861 aaggaggagc tcaaaaagct cgaagcattg gccaaagggc tggaggaacg tccgaacctc
     4921 cggttggaca tcacaggtac cgcggacacg gttcgcgatc gacaggcgct caatcgtatg
     4981 aaactccagg cgatcgtaca ggccaagtgg cagcgcgagc atgtgaagcc agccgcgaaa
     5041 gaagagccga taccagccgg tgacgagcag cggttgatcc aacaactcta cgaagcacac
     5101 cagaagaaca cgatccaatc cgccgcgcca cttacagttg atccatctgc taaaccgttg
     5161 acgtatgaag agcagaaaca cgtgttgata gccgctatgc ccttggatga agaggcactt
     5221 cgcggtttgg cccttcagcg tgccgaacaa gttcgtgaac aattgaccgg tgagggcaaa
     5281 ctggccgatg aacaggtcaa tctgctagac gtcgaattga acgaatcaga tcatgaccag
     5341 attcgaagta ggctggccat cgaagcggcg ccttgaggag tagaagctga gtccagtttc
     5401 ccctcttgaa gtatccggga cggcgcagta tcgtagagcg atcaacgcac gtagatcccc
     5461 attaccactg tttatagaac ggagggcaac atggcagtac gattgggaga tgaggcaccg
     5521 aatttcaccg cggagacgac agaagggacc gtcaacttcc acgaatggtt gggtggggga
     5581 tgggggattc ttttctcgca cccgaaggac tataccccgg tttgtaccac ggagttaggg
     5641 actgtcgcca agattacgcc ggagttcaag aagcgaggcg tcaaagttat cgctgtcagt
     5701 gtcgatccgc tggattcgca taaaggttgg atcaacgata tcaatgagac gcaaaagacg
     5761 acgatgaact atccgatcat cgccgatcct gataagaagg tcgcgaccct gtacgacatg
     5821 attcacccga atgcgatcga taatatgacg gtccgttcag tattcatcgt cgggcctgac
     5881 aagaaagtaa agttgacatt gacctatcct gcttcatgtg gccggaactt cgatgaatta
     5941 ttaagggtcg tggactctct tcaattgact tcgaagttca aagtcgcgac accggcaaat
     6001 tggaaagacg gcgaagattg catcatcact ccggcggtga atgacgctga agccaagaca
     6061 ctcttcccca agggcttcaa ggccgtcaag ccctacttgc gctatacccc gcaaccgaat
     6121 aagtaacgaa cggtttgact ggtactgagg gcgggagaag ctttgagctt ctcccgcctt
     6181 tcgtgtttca agcatcctgc tgtcggttct ctctaaatcc aaacatgcat gcttaatcgg
     6241 gtaatagtga gtcattcgtt tcattccttt ggggtccagc caaagacgat caccgccatc
     6301 ccaaacaaag caattccggc tccagtccag tcaaagacgg agagcttctc gccgtccacg
     6361 accctcagcc agacgagggc cgttgccaca tatacaccgc cataggcggc atagacgcgt
     6421 ccgctgg
//
LOCUS       DATIPF010000414         3219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_697553, whole genome shotgun sequence.
ACCESSION   DATIPF010000414 DATIPF010000000
VERSION     DATIPF010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697553"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1549
                     /locus_tag="VLL06_10335"
     CDS             242..1549
                     /locus_tag="VLL06_10335"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HSS31405.1"
                     /translation="MGNLVVIGAQWGDEGKGKIVDILASDVDAVVRYQGGSNAGHTVI
                     NDRGTFVFHLIPSGILYRGTLCVIGNGVVVDPASLIEELDHLQTQGVKIGKNFIVSHR
                     AHLILPYHKAIDKASEQSKGSRRIGTTGRGIGPSYADKMSRVGIRMGDLLNPKAFRTK
                     LEENVVEINWFLEQLYKLERFEVDHVFRQYMGYADRLKSHIVDATMLVNKMIDGGKTV
                     LFEGAQGTHLDVDFGTYPYVTSSSSTAGGASTGTGVGPTKIDAVLGVAKAYTTRVGSG
                     PFPTELTDEVGDGLQARGKEFGSTTGRARRCGWFDGVVVRYATKVNGLASLAVTKLDV
                     LDGCKELKVCTGYRYQGKLYRDMPADLQTLTDCEPVYKTFKGWSGTTTGASTYKQLPA
                     EAKRYLQFLEELSECPIDMISTGSKRSETIILRNPLNIRPRVR"
     gene            1615..1690
                     /locus_tag="VLL06_10340"
     tRNA            1615..1690
                     /locus_tag="VLL06_10340"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1648..1650,aa:Lys,seq:ttt)
     gene            2192..2332
                     /locus_tag="VLL06_10345"
     CDS             2192..2332
                     /locus_tag="VLL06_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31406.1"
                     /translation="MSNAKNLRKIFHEVQASGKLETYEFIAILSNLIDIADRLERVGD
                     RL"
     gene            2801..3217
                     /locus_tag="VLL06_10350"
     CDS             2801..3217
                     /locus_tag="VLL06_10350"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HSS31407.1"
                     /translation="MTKEPTVKNGHADVTTLERVLQWHGAFRMSLGQIRVTPLQAAML
                     LFVSRHAEAKLKDVARALCVRRPTLTEVVKALVRNRWVTQRRSVVDARVVHLQLSQRG
                     EALTRQIERQVHQVTITLAEEVRKSLGMTPQTRAVL"
BASE COUNT          726 a          875 c          913 g          705 t
ORIGIN      
        1 ggggctggac aaggcctctc gtgtcttgtg ctcgacgaac gtaccatcgg aaattttccc
       61 tcattcacgt gcacaacaag aaaatggatt gatcctgcct tcgaagatga tgaggaggca
      121 aaacgttgaa tagtgaactg cccttcacag tccttgacaa tcattagagg cgcccctacc
      181 atggcgctcc agccctggcc atgagtcgag ccagggcaat tttttgggag catgtcgggc
      241 gatgggaaat ctcgtagtga tcggggcaca gtggggcgac gaaggcaagg gcaagatcgt
      301 ggatatctta gccagcgacg tcgatgcggt ggtccgctat cagggcgggt ccaacgcggg
      361 acacacggtg atcaatgacc gaggcacctt cgtctttcac ctcattccgt caggcattct
      421 ctatcgtggg acgctctgtg tgatcgggaa cggggtcgtt gtggacccag cttcgttgat
      481 cgaggagctg gatcatctcc agactcaagg tgtgaagatc ggcaagaatt ttatcgtcag
      541 tcatcgcgcc catcttatcc tgccgtatca caaggccatc gacaaagcgt ctgagcagtc
      601 taagggctct cggcgaatag gcacgacagg gcgtgggatt ggcccatcct atgccgataa
      661 gatgtcccgg gtcgggatcc gaatgggaga tctcctcaac ccgaaggcct ttcgaaccaa
      721 actcgaagag aatgtggtcg agatcaattg gtttctcgag caactgtata agctggaacg
      781 gttcgaagtc gaccacgttt tccggcagta tatgggctat gccgatcggc tgaagagcca
      841 tatcgtcgat gccaccatgc tcgtgaacaa aatgatcgac ggcggaaaga ccgtgttgtt
      901 cgaaggggcg caagggacgc acttggacgt ggacttcgga acctacccct acgtcacctc
      961 ttccagttca acggccggtg gtgcctcaac gggaacgggt gtgggcccga cgaagattga
     1021 cgcggtactc ggtgtcgcca aagcctacac cacgcgtgtc ggtagtggac cattccctac
     1081 ggaattgacc gatgaggtgg gagacgggct ccaggcacgt gggaaagaat tcggatccac
     1141 gacgggacgt gcgcgccgct gcggctggtt cgacggcgtc gtggtgcgat acgccaccaa
     1201 agtcaacggg ctcgcgtcac tggccgtcac gaaattggat gtgctcgacg gctgcaagga
     1261 actgaaggtc tgcaccggct atcggtatca ggggaaactg tatcgagaca tgccggccga
     1321 tctccaaacg ctgaccgact gcgagccggt gtacaagacg ttcaaaggat ggtccggcac
     1381 aaccaccggc gcgtccacgt ataaacagct tccagcggaa gccaagcgct atctccagtt
     1441 ccttgaggag ctctccgaat gtcccatcga tatgatctcg accggctcaa agcgtagtga
     1501 aaccattatt cttcgcaatc cactcaacat ccgcccgcgc gtgcgataag ccattcattt
     1561 cctctctagg cacaggctga gcgtcggtgg tacaatccgc gctcttcgct cggtgagccg
     1621 gtagctcagt tggtagagca tcgccctttt aaggcgaggg tcctgggttc gagtcccagc
     1681 cggctcacca ttgttcaaat agttaggatc acccgccgcg accgccggtc gttcatagga
     1741 caatccatag gacattttga aggcgtggtc gaggatcggc acggcctcgc gaagcttgtg
     1801 gttccacgct ggaccaccgt gcgagtagtt cgcggtcaag cctggcatgc gatgtccgag
     1861 caggacttgc tgcgcttcat aatccacacc aaggccctac agctggggac catcacgaaa
     1921 tcattgcgaa gggcgatgct cgtgggcatc gggcctttat tttgcccggg tggaccaggg
     1981 tttttccgtg tttcgaaatg ccttgggggt tcatagaata aagacacttg agaacgccat
     2041 gcgcgcagat gatgatgcac atgtgcgcgc tatgagatcc ttgagatcct ggcggaatcc
     2101 aagtgggctc ggctccgaaa ggaaccgggc ctttattttg atcagatcct aaacctgtgc
     2161 agcttcagca ttttcatgga gggaattgga tatgtccaat gctaagaacc ttcgcaaaat
     2221 ctttcacgaa gtgcaggcaa gtgggaagct tgaaacatat gagtttattg ccatattgag
     2281 caatctcatc gacatcgctg accgcctaga aagagtggga gatcgcctgt aaaatagaag
     2341 gatcatgagg ccaacagcgt taaccgcgca gcatccccac aagtccttcc accgtcaacg
     2401 acgcattctc ctcggcccga ttgttgacca gagcacatac gctcgctggt tctcagccat
     2461 ggccttcctg accaattcca ccgtatccct cctcatctcc ggcagctctc cgacaatctt
     2521 gctgtacggt tctgctcgat tcttgacggc ttcgtaggct atttcttacg ccaagatcag
     2581 gatgtttcct acatccaaca cggcgcgttc tgtctaggct acgctgcagt gctctcgcac
     2641 cttctactat ggaagccctg tgccggtaca ctgtcctccg tgtgcatgca gtgcgactat
     2701 agcggaatct gaaataaagg ctcggctccg cgaacgggac cgggcctttt tgttatgcag
     2761 acgaccatac ttgtctagga agtcagggag catgttacct atgacgaaag agccaacagt
     2821 gaagaacggc catgcggatg tgactactct agagcgagtg ctgcaatggc atggcgcgtt
     2881 ccgaatgagt ctcgggcaaa tccgcgtgac gccgctccaa gctgccatgc ttctttttgt
     2941 gagtcgtcac gcagaggcca aactgaagga tgtggccagg gcgctttgtg tgaggcggcc
     3001 aaccttgact gaagtcgtca aggccctcgt acgaaatcgg tgggtgaccc aacgtcgatc
     3061 ggtcgtagat gctcgtgtcg tgcatttgca actgagtcag aggggtgagg cgctcactcg
     3121 tcaaatcgag cggcaggtcc accaagtgac tatcacactc gccgaagaag tacgaaaatc
     3181 cctcggcatg actccgcaga cccgggccgt cttgtgatc
//
LOCUS       DATIPF010000415         3971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1577232, whole genome shotgun sequence.
ACCESSION   DATIPF010000415 DATIPF010000000
VERSION     DATIPF010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577232"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VLL06_10355"
     CDS             <1..393
                     /locus_tag="VLL06_10355"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSS31408.1"
                     /translation="EHFGITGFCWGGRIVWLYAAHNPKLKAGVAWYGRLAGKPSTLQP
                     KHPIDQASSLKAPVLGLYGAEDSGIPLDTVEQMKQALHTAGSPSQIVVYPNTPHGFYA
                     DYRPSYRKEQAEDGWKRLQGWFKQHGVA"
     gene            1317..1847
                     /locus_tag="VLL06_10360"
     CDS             1317..1847
                     /locus_tag="VLL06_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31409.1"
                     /translation="MSKKKKRQANEQKPPITTKQPPPSSLKQVVKWISIAVLICTLLS
                     GLMTFASVLTIDAGTPLDSNNSFSYPFIVSNNMIVPLFGVTISCKYHDVEFEGGGTLD
                     KTAAGSEEMLFVMAPHQTTPAPCQKGMTMNAPLKSGIVTIAVNYSPFLWPFKTSTIRH
                     FKTEITADKKTLWLPQ"
     gene            2343..3968
                     /locus_tag="VLL06_10365"
     CDS             2343..3968
                     /locus_tag="VLL06_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HSS31410.1"
                     /translation="MSFPIEKVSSDELAREVARRRTFAIISHPDAEKTTLTEKLLLYA
                     GALHLAGAVQARSHQRAAKSDWMELEQARGISITSTALQFDYQGVRVNLLDTPGHQDF
                     SEDTYRTLMAVDSAVMVLDCAKGIEPQTKKLFAVCRKRGIPIMTFINKLDLPGRHPFE
                     LLEEIEQTLGMTAVPFNWPIGEGSGFQGLYDLQEPQVLFFQRTLHNQRRAPMRVEVFP
                     NPNLAETLGEAYGPLYEDIALLTGAGTKFDRDQFLAGQLTPVFFGSALTNFGIEPFLN
                     AFLQLAPPPGPRMSSQGPVQPANDKFSGFVFKIQANMDPQHRDRMAFLRIVSGRFEKD
                     MMVYHARLDRKVRMTRPHRLFGRDRETIEEAYPGDVVGLVNPGLFVIGDTLCSGSPLA
                     FDAIPQFPPECFGVLRSQDLTKQKQFQKGLQQLEEEGVMQVFYSPDHVRREPVLAAVG
                     ELQFDVVASRLETEYGVKTLVERMSFVLARWVSGDPDVLAGIYWPQDTRRLVDRNGRM
                     VMLFGSERLLAYCIREYPSLRFQLREELEKMET"
BASE COUNT          937 a         1131 c         1041 g          862 t
ORIGIN      
        1 gaacacttcg ggatcacagg cttctgctgg ggaggaagaa tcgtgtggct atatgccgca
       61 cacaatccca agctgaaagc gggagtggcc tggtatggac ggcttgccgg aaaacccagc
      121 acgttgcaac cgaaacatcc cattgaccaa gcctcatcct taaaagctcc ggtgctgggg
      181 ctctatggag ccgaagactc tgggattccg ctcgacaccg ttgagcagat gaaacaggcg
      241 ctgcacacag ctggtagccc ctcgcagatc gtggtctatc ccaacacccc gcatgggttc
      301 tacgcggact atcggccgag ttatagaaag gaacaggcgg aggatgggtg gaagaggctg
      361 cagggctggt ttaagcaaca cggtgttgcc tagtctgcag acgtgtccag agcggaaatg
      421 gcggggtggc atagccctga gcgacaacgc ctcccggaac ggcccattcg gactttgcca
      481 ggcggaggcc ttctgaaatt cctttacagt cagtttcagc tttccttcct ggcaaagtct
      541 cgatgggcac cccgttcctc cggtgatttc cgctccgggc tatgccaccc caccagagaa
      601 atctgcagaa actgagaacg taaaaagggg acaggccact tacaagcctg tcccttaacg
      661 cttcacctta ttcagtcctc tattgcgtga cggtgaaacg tgacggtaag ggatattttg
      721 tggcatatga ataggaggaa agcgagtgtg tccggaatcg gggcaaagcc acgccgagtt
      781 ccgctagcag ccgggaggtc agacgtcgtc aggtcgacga ctctgaaaag ctactgatct
      841 ttggcgattg agaagctcgg aacccagcag tcctattcgt cgttcctcct caattctagc
      901 tagtcgctga acatacgcat tcaacgccat gagcccgagg acaaccgcca gccccagaat
      961 ggcccagacg aggtctccca atgcccaatc cctatccgcc atcagagcaa ctccttggtg
     1021 agtgggtttt tcgacatagg atcttccagg caatacttca agctacacga acgtaaactc
     1081 atgtgctatg actttcaagc acgaagtctg ccactagtaa gaaggatggt ctgctggctc
     1141 cctatcccac gtttgagaaa tatgaatggg atgtgagtta tactctcgca tgtcgtcgcc
     1201 ttcgccggca aagagggcta cattctcgca tcaagcctgg ctctaggcga gaacaacgca
     1261 cacggaagcg caagttcaga acaaggtcca ccagctgatc atctcataca ccagccatgt
     1321 caaaaaagaa aaagcgacaa gcaaatgagc agaaacctcc cattaccaca aagcaacctc
     1381 ctccatcatc gctcaagcag gtcgtaaagt ggatatcgat agctgtactg atatgcactc
     1441 tcctctctgg tctgatgaca tttgcctccg tcctgaccat tgacgccggc accccactag
     1501 actccaacaa ttcattttct taccccttta tcgtgagtaa caacatgatc gtgccattat
     1561 ttggagtgac catttcctgc aaatatcatg acgtggaatt tgaaggaggg gggacactcg
     1621 ataaaactgc tgcaggttcc gaggaaatgt tgttcgttat ggcccctcac caaacaactc
     1681 ctgcgccctg tcagaaagga atgactatga atgctccgct gaagagcgga attgtcacca
     1741 tcgcggtgaa ttattctccc ttcttatggc catttaaaac gtccacgatc cgccatttca
     1801 aaacagaaat cactgctgat aagaaaaccc tttggcttcc ccaatgaagc atccggtcaa
     1861 ttactagata tcaaataagg cacccgtgct cttccaaact cgatcgttca tccctccaga
     1921 gggtggtctg atcgatcctc tattgcgcga tcttctcacc cacccaccct cgacgcgccg
     1981 agacgcgtcg cttggccatg cgagcacatt cttatcgtgc acattccgcg agtaggagga
     2041 cgcaggctac cctcctctcc ctcctgtatt ttctcctctc ccagatgata aaacatagct
     2101 cccatacctc agtaggttgc agatacgctc ggtgtatacg gagaatactg ttgcactttc
     2161 cgcatggtgt tggaaactga atcgcctcag gatgctcaaa aaggccgtcc ggcaaggccg
     2221 caggcgaatc gaaaccggag gcgtaccctc aggggtacgt tgaggatttc gatgagccga
     2281 gatcgacgct ggcggtcttt ttcagcatcc tgctagtccc gtgaatacga gaaggaatgt
     2341 ctatgtcttt cccgattgag aaggtgtcgt ctgatgaatt ggcccgagaa gtagcacggc
     2401 gacgcacgtt tgccattatc tcgcatccgg atgcggaaaa aaccacgctg actgagaagc
     2461 tcctcctcta cgccggtgca ctacacctgg ccggcgccgt gcaggcacga tcgcatcagc
     2521 gggcggcaaa atcagactgg atggaactgg agcaggcgcg cgggatttca atcacgtcca
     2581 ccgcactgca gttcgattat cagggtgtgc gcgtcaatct gctcgatacg ccgggccatc
     2641 aagatttcag cgaagatacc tatcgcactc tcatggccgt ggatagcgcc gtcatggtgc
     2701 tcgactgtgc caaaggcatc gagccgcaga caaagaaact gtttgctgtc tgtcgcaaac
     2761 gaggtatccc gatcatgacc ttcatcaata aattggatct gccgggacgc catccgttcg
     2821 aactgctaga agagatcgag cagaccttgg gcatgacggc tgtgccgttc aattggccca
     2881 tcggggaagg gtcaggattt cagggactgt acgatcttca agaacctcaa gtgctgttct
     2941 ttcagcggac actgcataac caacgccgcg cgccaatgcg agtggaggtc ttccccaatc
     3001 cgaatctggc ggagacgctg ggagaggcct acggtccgct atacgaagac atcgccctgc
     3061 tgaccggcgc gggcacgaag ttcgatcgcg accaatttct tgccggccaa ctcacaccag
     3121 ttttctttgg gagcgccctg acgaacttcg gcatcgagcc atttctgaac gcctttctcc
     3181 aactcgcgcc cccgccagga ccacgtatga gttcgcaagg gcccgtgcag ccggcaaacg
     3241 acaagttctc cggcttcgta ttcaagatcc aggcgaacat ggatccgcag catcgtgatc
     3301 gaatggcgtt tttgcgtatc gtctctggcc gattcgagaa agatatgatg gtctatcatg
     3361 cgcgattgga ccgaaaagtt cggatgacgc ggccgcaccg gctcttcggc cgtgaccggg
     3421 agacgattga ggaagcctac cccggcgatg tcgtcgggct cgtgaaccct ggcctctttg
     3481 tcatcggcga cacgctctgc tctggctctc ctctcgcctt cgatgccata ccgcaatttc
     3541 cccccgagtg ttttggtgta ctcagaagcc aggatctcac gaagcaaaaa cagttccaga
     3601 aggggctcca gcagctcgaa gaagaagggg tcatgcaagt gttctattcc cccgatcatg
     3661 tgcgccggga gccggtcttg gccgccgtgg gcgaactcca atttgatgtc gtcgcgtcgc
     3721 gcctcgagac cgagtacggg gtcaagaccc tcgtcgaacg gatgtccttt gtgctggcac
     3781 gctgggtgag cggcgatccc gacgtactgg ccggcatcta ttggccgcag gacacgcgcc
     3841 ggctcgtaga tcggaacggc cgcatggtca tgctcttcgg ctccgaacgc ctgctcgcct
     3901 actgcataag agagtatccg tccctcaggt ttcaattgcg ggaggaactc gagaaaatgg
     3961 aaacctaaca g
//
LOCUS       DATIPF010000416         3918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_78545, whole genome shotgun sequence.
ACCESSION   DATIPF010000416 DATIPF010000000
VERSION     DATIPF010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78545"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VLL06_10370"
     CDS             complement(<1..1031)
                     /locus_tag="VLL06_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSS31411.1"
                     /translation="MVKPGVVSAVFMLISLWPALSMAEWGKPLGGTYDGPEGKPRIVT
                     PSHAELGADERATMAVFEQATKSVVFIANTAIQRDPWSLNLFEAPQGSGSGFVWNKQG
                     HIVTNFHVVYGANSITVTLADRTEHKATLIGADPDHDVAVLQIRAPEETLPPLSVGTS
                     HDLRVGQKVLAIGNPFGLDHTLTTGVVSALGRTIKSLSNRTIEGVIQTDAAINPGNSG
                     GPLLDSAGRLIGVNTQIVSPSGAFAGIGFAVPVDTINRIVPELIKHGKLIRPGLGVSL
                     VPDAMAKRWGIKGLIIGKVSRGSGAEQAGLKGARETIAGRVELGDIITAVDGKPVETL
                     DNLMDVMER"
     gene            complement(1034..1456)
                     /locus_tag="VLL06_10375"
     CDS             complement(1034..1456)
                     /locus_tag="VLL06_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HSS31412.1"
                     /translation="MAVKTVSLRLTMQGDTQIENITKSAVDALAGTGLLAGIVTVFVK
                     HTTASVMIIEDEPGIRADTKTFWDRALPADPAWQHNLRNAGEDNGHSHLRGQLQGPSV
                     TIPFLDGVLLLGTWQQIVLVDFDTRPRTRELIIQIMGE"
     gene            complement(1458..2324)
                     /locus_tag="VLL06_10380"
     CDS             complement(1458..2324)
                     /locus_tag="VLL06_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31413.1"
                     /translation="MTSTSRCAPLLVPRSLVAQLMALYDYPHPSQRGRIIRGYDRRHA
                     ARTARMCAAVATALGHGAERVRQYQIACLLHDLGRAGLDRQLFGKIWSWAKEHGIPTR
                     PREWRALHHDTPYGRETEAFLRRYRKDLEADGIPISAWAKEQVEMRLGYSRRLARRLR
                     AVRPAIKEMGVIWAPWMQQVMLYYYYPEKLASAKPWVRQLAEILVACEQFEAYSNQRR
                     GRDYYMRKKETLVDAFAYLETLQQDGIVSGPVMEVLRRLTAQGEFDEILEEARGRVFT
                     RNERRALRAMES"
     gene            2494..3138
                     /locus_tag="VLL06_10385"
     CDS             2494..3138
                     /locus_tag="VLL06_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31414.1"
                     /translation="MPRYMTLCRLSFAAILLGQLWPMVPESYAGPLDQLTDLTGRATV
                     VVTLKGRDNFNSEYLYDVSVKNLSADTFIGDSLYIVLDKVTNIGGEDRENLNSDTILS
                     RMEVLGQSGETQDGKPYFQIPGGAATDLTPSSQSLPTSVRLRNKDYLIVFTPSFKVFG
                     LKRPPPEPKQTPAAPVQPITTPTAPNRNSIDKLIQLLIKKGVLTEEEWRKANQP"
     gene            3135..3563
                     /locus_tag="VLL06_10390"
     CDS             3135..3563
                     /locus_tag="VLL06_10390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HSS31415.1"
                     /translation="MTDHTCRTCQGSWPRKDHFIADLGLSNAYLHDDQFFPGWTVVVF
                     KRHATELFHLAPTERIQLMEEVNLVAKTLAQVYEAKKINYELLGNQLPHIHWHVIPRL
                     NGDPSPLEPVWRVEHEPILRSGPDLQSTVQRLQQAIQNAR"
     gene            3674..>3918
                     /locus_tag="VLL06_10395"
     CDS             3674..>3918
                     /locus_tag="VLL06_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31416.1"
                     /translation="MRRRLVLVTLVLAAFSVQLSIHASFAQAPDEQSPPPSVIESPAP
                     LPPDDEPVISEFTRSEQEDPVFILSELRAKVRASPNNA"
BASE COUNT          867 a         1233 c         1014 g          804 t
ORIGIN      
        1 tggcgctcca tcacatccat cagattgtcc agggtttcga ccggcttacc gtcgaccgcc
       61 gtaatgatat cacccaattc cactcgcccg gcaatggtct cacgtgcccc cttcaatccg
      121 gcctgctctg ccccggaccc acgcgacact ttcccgataa ttaatccctt gatcccccat
      181 cgcttggcca tggcatcggg cacgagcgag acccccagcc ctggacggat cagcttgccg
      241 tgcttaatca actccgggac gatgcgattg atcgtgtcga ccggcacggc aaaaccgatt
      301 ccggcgaagg ccccgctcgg actgacgatc tgcgtattca cgccaatcaa tcgaccggca
      361 ctatcgagta acggtccacc ggagtttccc ggattgatgg cggcatcggt ctgaataacc
      421 ccttcaatcg tcctattcga caaggacttg atcgtccggc ccaaggcact cactacgccg
      481 gtcgtcagtg tgtgatcgag gccgaatgga ttcccgatcg ccaacacttt ttgcccgacg
      541 cgcagatcat gcgacgttcc gacggagagg ggcggcagcg tctcttcagg tgcacgaatc
      601 tgcagcaccg cgacatcgtg gtcgggatcc gcgccaatca gcgtggcctt atgttccgtc
      661 cggtccgcca gcgtgacggt gatcgaattg gctccgtaga cgacatgaaa attggtgaca
      721 atgtgcccct gcttattcca gacaaatccc gatccggacc cttgcggagc ttcaaagaga
      781 ttcaacgacc aggggtctcg ttgaatcgcc gtattcgcga tgaagaccac ggatttcgtc
      841 gcctgctcga acaccgccat ggtggcccgc tcatcggcgc ccaactcggc atgtgagggc
      901 gtgacgatcc gcggcttccc ttcaggcccg tcgtaggtcc cacccaacgg ctttccccac
      961 tccgccatcg acaaggcggg ccagaggctg atgagcatga ataccgccga aacgactccc
     1021 ggtttcacca tgatcattca cccatgattt ggataatgag ttccctggtt cggggtctgg
     1081 tatcgaaatc gacgaggacg atctgttgcc atgtccccag cagcaatact ccatccagga
     1141 aggggatggt cactgacggt ccttggagct gccctcgcaa atggctgtgg ccgttatctt
     1201 ccccggcatt tctcagattg tgctgccagg caggatcggc tggcagggcc cggtcccaga
     1261 atgttttggt atcggctctg atacccggtt catcctcgat aatcatcacc gacgcggtgg
     1321 tgtgcttgac gaacaccgtg acgatcccgg ccaacagccc ggtacccgcc agggcatcga
     1381 ccgcggactt cgtaatattt tcaatctgcg tatccccctg catggtgaga cgcaacgaca
     1441 ctgtcttaac cgccatctta tgactccatc gctcggagcg cacggcgctc gttccgtgta
     1501 aacacacggc cacgagcctc ctcaagaatt tcgtcaaact cgccttgcgc cgtcaatcgg
     1561 cgcaggacct ccatcaccgg gccactcaca ataccatcct gctgcaacgt ctccaaatag
     1621 gcaaaggcgt cgaccagtgt ttccttcttt cgcatatagt aatctcgtcc tcgacgttga
     1681 ttactgtagg cttcaaactg ttcgcaggcg acaaggattt ccgccaactg ccggacccag
     1741 ggcttggcag acgccagctt ctccggatag tagtagtaga gcatcacctg ctgcatccaa
     1801 ggcgcccaga tcacccccat ctccttgatg gcgggccgca ccgcacgcag tcgacgggca
     1861 agacggcgcg aatatcccag ccgcatctcg acctgttcct tcgcccaggc actgatcgga
     1921 atgccatcgg cctcaaggtc ttttcgatag cgccggagaa aggcctctgt ttcgcgtccg
     1981 tagggagtat cgtggtgtag ggctctccat tcgcgtggtc ttgtcggtat cccgtgctcc
     2041 ttggcccagg accaaatctt cccaaatagt tgacgatcga gcccggcacg gcccaagtca
     2101 tgcaacagac aggcaatctg gtactggcgg acccgctcag ctccatggcc cagcgccgtc
     2161 gccaccgctg cgcacatgcg cgcagtccgc gccgcatgac gccgatcata tcctcgaatg
     2221 atacgaccgc gttgcgaggg atgaggatag tcgtagagcg ccatgagctg cgccacgaga
     2281 gaacggggaa ccaggagcgg agcacatcgt gaagtggatg tcattcgtgg taacagtgga
     2341 agagccggca caaaaccaac tgcggcgaga atagcgttca cggaaagaga ctgtcaaggt
     2401 gatcgatcgc gcagaaggct cgctcaaccc gccgatcttt gctatgctca gatcggttga
     2461 aggcgagggg tttcacgatc ttggaagaga accatgccga gatatatgac gctctgtcgg
     2521 ctcagttttg cagccattct cctcggtcaa ttgtggccga tggtgccaga aagctatgcc
     2581 gggccgctgg atcagctcac ggatttgacc ggcagggcca cagttgtcgt caccctcaaa
     2641 ggacgggata acttcaacag cgagtacctc tatgatgtga gcgtcaagaa cctgtcggcc
     2701 gatacgttca tcggcgactc cctgtatatt gtcctcgaca aagtgaccaa catcggaggg
     2761 gaagaccggg aaaacttgaa cagcgataca atcctgagcc gaatggaagt cctgggtcag
     2821 agtggagaaa cacaggatgg aaagccctac ttccaaatcc caggcggagc agcgaccgac
     2881 ctaactcctt ccagccagag tcttccaacc agtgtacgtc tccgcaacaa ggactatctg
     2941 atcgtcttca cgccctcgtt caaagtgttt gggctcaaac gaccgccgcc tgagccaaaa
     3001 caaaccccgg ctgccccggt tcagccgatc accactccga cagcacccaa tcgcaactcc
     3061 atcgacaaat tgattcagtt gctgattaag aagggtgtcc tcaccgaaga ggaatggcgc
     3121 aaggccaatc aaccatgacg gatcatactt gtcgtacctg ccaaggcagt tggccgagaa
     3181 aggaccattt catcgccgac ctcggactgt caaacgccta tcttcatgac gatcaattct
     3241 tccccggctg gaccgtcgtt gtcttcaagc gccacgcgac agagctgttt catctggctc
     3301 ccacagaacg gattcaactg atggaggaag tgaatctcgt cgccaaaact cttgcccagg
     3361 tctatgaagc caagaagatc aattacgaac tccttggcaa ccaactcccc cacattcact
     3421 ggcatgtgat tccccggctg aacggcgacc catcacccct tgaaccagta tggcgggtag
     3481 agcatgagcc gatactccgt tctgggccag acctccagag cacggttcaa cgcctgcagc
     3541 aggccattca gaacgcccgc tgaatcacga atcgccggtc cactcaatgc actccgcccg
     3601 acccacgggc atcaattcgg tgatataata cagcccatct ttgacaacag atccggaggg
     3661 tcacaggttt ccgatgcgtc gccgattggt gctcgtgact ctcgtacttg cagccttcag
     3721 cgtacaactc tctatccatg cctcgttcgc gcaggcgcct gacgaacaat cccctcctcc
     3781 ttccgtaata gagtctcccg ctcccctgcc cccagacgac gaaccggtta tttctgaatt
     3841 caccaggtca gagcaggaag acccggtgtt catcctgagt gagttacgcg cgaaggttcg
     3901 cgcgtcgccg aacaacgc
//
LOCUS       DATIPF010000417         8942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_168656, whole genome shotgun sequence.
ACCESSION   DATIPF010000417 DATIPF010000000
VERSION     DATIPF010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168656"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /gene="obgE"
                     /locus_tag="VLL06_10400"
     CDS             complement(<1..892)
                     /gene="obgE"
                     /locus_tag="VLL06_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HSS31417.1"
                     /translation="MFVDEVRIHIAAGRGGDGSCSFRREKFVPRGGPDGGDGGHGGDV
                     VFEASSRMTTLLDIRYQKHYEAEVGRQGSAANCSGRTGEDVVVALPVGTVIFDDQTGE
                     VLADLVTPGQRFVGAHGGRGGRGNSHFATSTNRTPTEFETGTEGEERSLRLELKLLAD
                     VGLVGLPNAGKSTLIAAISAARPKIADYPFTTLTPNLAIVRWGEKESFAVADIPGIIE
                     GAHEGKGLGFQFLRHIERTSFLLHMIDVSEWAPEEPVKSLEIMRQELAAYDEPITTRP
                     FAVVATKIDVAGDKARLRALQ"
     gene            complement(1018..1281)
                     /gene="rpmA"
                     /locus_tag="VLL06_10405"
     CDS             complement(1018..1281)
                     /gene="rpmA"
                     /locus_tag="VLL06_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HSS31418.1"
                     /translation="MATNKGGGSTRNGRDSNPQYLGVKAYGGETVKAGSILIRQRGTK
                     FFPGFNVGLGKDHTLFARIAGIVKFERGRGRQKVSVYSAPSVS"
     gene            complement(1294..1608)
                     /gene="rplU"
                     /locus_tag="VLL06_10410"
     CDS             complement(1294..1608)
                     /gene="rplU"
                     /locus_tag="VLL06_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HSS31419.1"
                     /translation="MYAIIETGGKQYRVETGSLVQVESLPGEVGGTIELGQVRLVHGD
                     KGVEIGQPLVTGATVKAEIISQGRTRSITIFKKQRRKNYRRTNGHRQGFTKLRITGIE
                     TA"
     gene            1833..2207
                     /locus_tag="VLL06_10415"
     CDS             1833..2207
                     /locus_tag="VLL06_10415"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS31420.1"
                     /translation="MAAAPNPRLSKSKRTPFVLRPYRRIPTWLVLYYLSGESVGKGIV
                     TNLSRSGMRVQGDHAVEPGLDLGLRLTLEDNGTTIEIERATVRWVDGYDFGLDLVRFS
                     PDVAMHIARLVSQQIRAPQMPH"
     gene            2323..3237
                     /locus_tag="VLL06_10420"
     CDS             2323..3237
                     /locus_tag="VLL06_10420"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HSS31421.1"
                     /translation="MLEPQGKIWMDGSFVNWADAHVHVMTHSLHYGLAVFEGIRCYKG
                     KSGSAIFRLQEHVDRLFDSTHIGMMKMPFDKKQVAEAIVETVRVNKLDACYIRPLVYI
                     GHGAMGVYPGENPIKLAIAAWTWGAYLGEDALANGMRARVSSFARHHVNVSMTRGKIS
                     GYYVNSILAKREAKADGYDEAILLDTDGYVSEGTGENVFIVRKGQMKTTPLTSILDGI
                     TRSSVIDLAREQHISVVEERFTRDDMYIADEVFVTGTAAELTPVREIDNRCIGTGRPG
                     PITQALQKAFFSIVRGEDPSHAQWLTRV"
     gene            complement(3445..3807)
                     /gene="secG"
                     /locus_tag="VLL06_10425"
     CDS             complement(3445..3807)
                     /gene="secG"
                     /locus_tag="VLL06_10425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HSS31422.1"
                     /translation="MYTLLVVIHVLICFLMVGAILLQSGKGAEIGASFGGSSQTVFGS
                     RGPANFLSKFTVAVAAVFMLTSLFLAILAKEQAFSSTVIDLKKKEASQTAPEAPATTP
                     AAPTTESHPPSDSSSSGH"
     gene            complement(3822..4592)
                     /gene="tpiA"
                     /locus_tag="VLL06_10430"
     CDS             complement(3822..4592)
                     /gene="tpiA"
                     /locus_tag="VLL06_10430"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HSS31423.1"
                     /translation="MRRPLIVGNWKMNKTATEAVAFIRDFRNRIPASPHADVVIAPPF
                     TALTSACNALGPSSWISLSAQNVHWEPYGAFTGEISAPMLRELGCRYVIVGHSERRAL
                     FGERDETIHKKVRAALTHGLSPILCVGESLEEREASRTESVVTTQLGNSLAGLTTQDL
                     ATVTIAYEPVWAIGTGRAATTEQAVTVHRSIRLFIETGWDSPTASAMRILYGGSVTPR
                     NIEPFLASDAIDGALVGGACLDPDSFATIVRIAQTQRA"
     gene            complement(4650..5849)
                     /locus_tag="VLL06_10435"
     CDS             complement(4650..5849)
                     /locus_tag="VLL06_10435"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HSS31424.1"
                     /translation="MRLRKQTIDDVVLRDKRVIIRADFNVPLDDSHQITDDTRIRSTL
                     PTINRAIDDGARVILCSHLGRPNGKFDPKYSLAPVAKRLGRLMGKDILFAPDCIGPAV
                     ETLVAKMKPGDVLLLENLRFHQGEEDNDDAFSKALATLGDVYINDAFGAAHRAHASTV
                     GITKFIPASVAGFLLKKEIEYLEGAVENPVRPFVAILGGAKVSGKIGVIENLGKRVDK
                     VIIGGGMAFTFLKAKGMEIGNSLVENDMLALAKGIEDHALSRGVKFYLPVDCVVAASR
                     ELGAESKIVPVQEIPNGWYALDIGPASVKLFNEAVQNAKTILWNGPMGVFEIDAYARG
                     TLAMAHAIADAYALTIVGGGETALAVHRSGESENMSFISTGGGAALELLEGKQLPGLT
                     ALPNRVT"
     gene            complement(5934..6944)
                     /gene="gap"
                     /locus_tag="VLL06_10440"
     CDS             complement(5934..6944)
                     /gene="gap"
                     /locus_tag="VLL06_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HSS31425.1"
                     /translation="MAIRIGINGFGRIGRNVLRASLGDPDLHFVAINDLTDAKTLAYL
                     LKYDSVHGTLPGSVEAKGDQILVDGKPIKVLAMKDPKELPWKDLDVDIVIESTGRFTD
                     REGAGKHLSAGAKHVIISAPAKDPDVTIVLGVNDAAYDPNKHHIISNASCTTNCLAPV
                     AKVLLDNFGIKHGLMTTIHSYTNDQQLLDLPHKDMRRGRAAGMSMIPTSTGAAKALHL
                     VLPQLKGKIDGLAIRVPTPNVSLVDLTVETEKDCDLASVNGAFRKAANGPMKDILLYS
                     EDPIVSIDQKGDPHSATFDSPLTMVIDKRLVKVTAWYDNEWGYSCRVRDLVKVIAART
                     RR"
     gene            7198..>8942
                     /locus_tag="VLL06_10445"
     CDS             7198..>8942
                     /locus_tag="VLL06_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31426.1"
                     /translation="MNLLEQTTMVLEWPRLLEALAGHARSTMGAAGCRALELTTSLHD
                     ARRRQQETTEMGQLQASGEDLPTLAFPDIRDPLARAKKGAALEAHELRDCAVVLGLVE
                     EIGRFVRRHQHDAPALASVANHLPGGRELHSVKAALDAAIHPDGSIKESATPELRRST
                     QQAQSLKQQMRNDLDQILHSRRYQDILQELYFAQREGRYVIPVKADMRGRVPGIVHDI
                     SASGATVFLEPRELVELNNSIKVADLEIEREVRRILRELSALVAAQSEVVLAGLEALA
                     VLDGISARASFSRQLKAHPVGLNAEGRVRLLRARHPLLVLSKEQVVANDILLGESIQV
                     LVISGPNAGGKTVTLKIVGLFALMVRAGLHLPCDAGSEMAFFSNVCADIGDAQDLARD
                     LSSFSAHMTQMIQLLNETDCRRRDDEHGAPRQWLVLLDEPVTSTDPTEGAALAEALLC
                     RLATLGMKVVATTHYGSLKALAHTMPGFANASVEFDVATLSPTYRLFMGVPGGSSALE
                     IAGRLGIDRALLDEARKKLHKDERAMETMLHDLQATQRRLADDLARAVEARREAEQAE
                     QRAKAQLAHLEETER"
BASE COUNT         1807 a         2435 c         2650 g         2050 t
ORIGIN      
        1 tctgtaacgc gcgcaggcgc gccttgtctc cagctacgtc gattttcgtc gcgaccaccg
       61 cgaaggggcg tgtggtgatc ggttcatcgt aggcggctaa ttcctgacgc atgatctcaa
      121 ggctcttcac aggctcctca ggagcccatt ccgacacatc aatcatgtgc aacaggaaag
      181 acgttcgttc gatatgccga agaaattgga atcccagtcc ctttccttca tgggctcctt
      241 cgatgattcc cggaatatcg gcgacagcga aactttcttt ctctccccag cgaacgatcg
      301 ccagattcgg cgtcagcgtg gtgaaggggt aatcagcgat cttcggccgt gcggccgaga
      361 tcgcggcgat cagagtcgat ttcccggcat tgggaagacc cacgagcccg acgtcggcca
      421 acagtttcaa ctcgagtctg agcgaccgtt cttcgccttc cgtgccggtt tcaaattcgg
      481 tgggagtgcg gttggtcgac gtggcgaagt ggctattgcc ccgaccgccc cgacccccgt
      541 gggctcctac aaatcgctgt cctggggtca ccaggtctgc caacacctca ccggtctgat
      601 catcgaagat gacggtgccg acgggaaggg ccactaccac atcctctccg gtccttccgg
      661 aacaattggc tgcgctaccc tggcgaccga cttccgcctc atagtgtttt tggtagcgga
      721 tgtcgaggag cgtggtcatg cgagacgatg cttcaaatac gacatcgccg ccgtggccgc
      781 catcgccgcc gtccggtccg ccgcgcggca cgaatttttc gcgtcgaaaa ctgcatgagc
      841 cgtcgccgcc gcggccggct gctatatgga tacggacttc atcgacaaac atcggtgcac
      901 cgtttcagtg tgatcgctgt gtaggcatga cctgtgagga acatcccgcc gcacctctgc
      961 tcatgccggg agtccttgcg gtaacgacac tgtgacgaga aagtaaggaa agatgcgtta
     1021 tgaaactgaa ggcgcggagt atacactgac cttctgtcgc ccacggccgc gttcaaactt
     1081 cacgatcccc gcgatgcggg cgaagagggt gtggtcttta cccaggccta cgttgaaacc
     1141 gggaaagaac ttcgtccccc gctgacggat gaggattgat ccggccttca cggtctctcc
     1201 accataggcc ttgaccccaa gatattgcgg attgctatcg cgcccatttc tcgttgatcc
     1261 gccgccttta tttgttgcca tgactgatcc tcgttaagcc gtttcgatac cggtaatccg
     1321 cagtttcgta aagccctggc ggtggccgtt agtccgccga tagttcttgc gccgttgttt
     1381 cttgaagatg gtgatggagc gtgttcgacc ctggctgata atctcggcct tgacggtggc
     1441 ccccgtgacc aacggttgtc cgatctctac acccttgtcg ccatggacca gccggacttg
     1501 gccgagttca atcgtgcctc cgacctcgcc aggaagggac tccacttgga ccaatgatcc
     1561 agtttcaaca cgatactgct tgccgcctgt ttcgatgatc gcgtacataa gcccttgtct
     1621 ccattacgga agacgatctt gtaacatagc catttgaagc ctgtcaaggc agaggcagat
     1681 ctcttcagga aaatctcatt tcctctccga tcgagttcag tcgacaagcc gtacccctcc
     1741 tacgggttaa cctattcggg ggctatcctg ctctgacttc tgggcgtatg ttgttgatct
     1801 attgttgagt cattctggga ttggagatca taatggccgc cgcaccaaac cctcgtctct
     1861 ccaagtcgaa acgcactcca tttgtgctcc gtccatatcg acgtataccg acctggcttg
     1921 ttctctatta tctcagtgga gaatctgtcg ggaagggtat tgtgacaaat ctgtcccgat
     1981 cggggatgcg tgttcaaggc gaccatgcgg ttgagcctgg tctcgatttg gggctgcgcc
     2041 tcacgcttga ggacaatggt acgacgatag agattgaacg agcgacagtt cgatgggtcg
     2101 atgggtatga ttttgggctg gatcttgtcc gattcagtcc ggatgtcgcg atgcacattg
     2161 cgcgattagt cagtcagcag attcgtgccc cgcagatgcc ccactgaaag attgcgttgt
     2221 gattcgttct tgccagtcgt tcaagccacg cccatttgct tgacgcttct tccagcccgc
     2281 tgctatattc cgattttgac gctcaatagt tctttgctgt ctatgcttga gccacaagga
     2341 aaaatttgga tggacgggtc attcgtcaat tgggcggacg cgcacgtcca cgtgatgacc
     2401 cattcgctcc attatggact ggctgtgttc gaggggattc gttgttacaa ggggaagtcc
     2461 ggctccgcga tttttcggtt gcaggagcat gtagatcggc tgttcgactc cacccatatc
     2521 ggcatgatga agatgccctt cgacaagaag caggtcgcag aggccatcgt ggagacagtc
     2581 agggtgaata agctggatgc ctgctatatt cggccgctcg tctatattgg gcatggagcg
     2641 atgggggtct accccggtga gaacccaatt aagttggcca tcgcggcttg gacttggggt
     2701 gcctaccttg gggaggatgc gttggcgaat ggtatgcgag cccgggtctc atcgtttgct
     2761 cgtcatcacg tcaatgtatc gatgacgagg gggaagatct caggctacta cgtgaactcg
     2821 attcttgcga agcgggaagc gaaagcagac gggtacgatg aagccatttt actcgatacc
     2881 gatgggtatg tgtccgaagg gacaggggag aatgtcttca ttgtgcgcaa aggacagatg
     2941 aagaccacgc cgcttacctc gattctcgac ggcattacca gaagctccgt catcgacttg
     3001 gcgagggaac agcatatctc cgtggtggag gagcgcttca cgcgcgatga catgtatatt
     3061 gctgatgaag tattcgtgac tggaactgct gccgaactga cgccggtgcg ggagatcgac
     3121 aaccgctgta tcggaaccgg taggccaggt ccaatcacgc aagctctgca gaaggccttc
     3181 ttttccattg tgcgtgggga ggacccttcc cacgcacaat ggcttacccg cgtgtagcag
     3241 gaggctgaac agtttcagct tttcccccat atctcacgct ccgccttctc aagcgattgc
     3301 tccggcaaca ttttcgaatg cgtgattcgt ttttcattat tctttcgaaa agtcggacgg
     3361 gcagaagtct cctcttcgca agtacacatc cgtcatactc gcacatactt cacgagtgac
     3421 gaggactttg ttttcgatat tctgctagtg tccgctggag gaagagtcgc tgggggggtg
     3481 gctttcagtg gtcggggctg ccggcgttgt tgctggggct tcgggggcag tctgagaagc
     3541 ctctttcttt ttgaggtcga tgactgtgga ggaaaatgcc tgttcttttg ctaggatggc
     3601 tagaaaaagt gaggtcagca tgaagacggc agcgacggcg actgtgaatt tgctcaagaa
     3661 gttggcgggg cctcggctac cgaagaccgt ctgactagag cctccgaatg atgcgccgat
     3721 ctcggcaccc ttccctgatt gtaataggat tgcgccaacc atgagaaaac agatcaacac
     3781 atgaatcacg acgagaagcg tatacatggc actccgatca gttaggccct ctgtgtctga
     3841 gcaatccgga cgattgtagc aaacgagtcg ggatcgagac aagccccacc caccagagcc
     3901 ccgtcaatcg catccgaggc aagaaacggc tcgatgttcc ggggcgtcac gcttcctccg
     3961 tacaagattc tcatggccga cgcggttggg ctatcccagc cggtttctat gaacaagcga
     4021 atcgagcgat gcacggtcac cgcctgctcc gtggtggcgg ctcgtcccgt gccaatggcc
     4081 caaaccggtt cgtaggcaat tgtgacggtt gccagatcct gtgtggtcaa cccggcaagg
     4141 ctgttgccaa gttgagtggt gacgaccgat tccgttcggc ttgcctcacg ttcctccagc
     4201 gattctccca cacacagaat cggagagagt ccgtgcgtca gcgcggcccg gaccttcttg
     4261 tgaatagtct catctcgttc gccgaagagg gcgcgacgtt cggagtggcc cacgatcaca
     4321 tagcggcagc ccagttctcg cagcatagga gcggatattt cccccgtgaa ggccccatac
     4381 ggttcccagt gcacattttg tgcgctgaga ctgatccagg acgaggggcc taacgcgttg
     4441 cacgccgagg tgagggccgt aaagggtggc gcaataacca cgtccgcgtg aggcgatgca
     4501 ggtattcgat tgcgaaagtc gcgaatgaag gccacggcct ccgtggccgt cttgttcatt
     4561 ttccagttac cgacaatgag gggtctacgc acgagtgtct cgttagtcat agaggtgtga
     4621 ttgctgcagg ctgctccgcg gcgaggcgat tacgtgactc gattcggaag ggcggtcaag
     4681 cctgggagct gtttgccttc gagcaattcc agcgcagcac cgccgccggt cgagatgaac
     4741 gacatattct ccgactcgcc ggatcgatgc acggccagag ccgtttcacc tccgccgacg
     4801 atcgtcagtg cataggcatc ggcaatcgcg tgggccatcg cgagggtgcc tcgggcgtac
     4861 gcatcaattt caaagacccc catcggtccg ttccacagaa tcgtcttggc gttctgaacc
     4921 gcttcgttga agagtttgac ggaggccggg ccaatgtcga gcgcatacca cccgtttgga
     4981 atctcctgga ccgggacgat cttcgattcc gccccaagtt cgcggctggc cgctacgaca
     5041 caatcgacag gcagatagaa tttgacaccg cgggacagag catggtcctc aatgcctttt
     5101 gcaagggcca acatgtcgtt ttcgaccagg gagttgccga tctccattcc cttcgccttt
     5161 aagaaagtga aagccatgcc gccgccgata atgaccttgt cgacgcgttt gccaagattt
     5221 tcgatgacgc caatcttccc cgatactttt gccccgccga gaatggcgac aaatggccga
     5281 acgggatttt cgacggctcc ctcgagatac tcgatttctt tcttgaggag gaaaccggcg
     5341 accgaagcgg gaatgaattt ggtgataccg acggtggagg catgagcccg gtgtgcggcg
     5401 ccgaatgcat cattgatata gacatcgccg agagtggcca gcgcttttga gaacgcgtcg
     5461 tcgttgtctt cttcaccctg gtggaatcga aggttttcaa ggaggagtac gtcccccggt
     5521 ttcatctttg cgacaagcgt ttccactgcc gggccgatgc aatctggcgc aaacagaata
     5581 tctttgccca tgagccgtcc caggcgtttg gccaccgggg ccaggctgta cttggggtcg
     5641 aactttccgt tcggtcggcc caggtgggaa cagaggatga ccctcgcgcc gtcatcgatg
     5701 gctcgattga ttgtcggcag cgtcgagcga atgcgggtgt cgtcggtgat ttgatgcgaa
     5761 tcatctaagg ggacgttgaa gtcggctcgg ataatgacgc gcttgtcccg cagtacgacg
     5821 tcgtcgatgg tttgtttgcg gagtctcatg gcggttctgt caggttagtc cttccaggga
     5881 tggggttcag ggtgaaacag aatagggcgg aggcgaggta tcgcctcgcg gtgctagcgc
     5941 cttgttcttg ccgcgatcac cttgactaga tctcgaaccc ggcatgaata gccccactcg
     6001 ttgtcgtacc aggctgtcac cttgacgagc cgcttgtcga tgaccatcgt gagcggggaa
     6061 tcgaatgtcg ccgaatgagg gtcgcccttc tggtcgatcg agacgatcgg gtcttcagag
     6121 tagagtagaa tgtctttcat ggggccgttc gctgccttcc ggaaggcgcc attgacggag
     6181 gcgaggtcac agtctttttc ggtctcgacg gtcagatcca cgagcgagac attcggcgtt
     6241 ggaacgcgga tggcgaggcc gtctattttc cccttgagct gtggtagaac caaatgcagc
     6301 gcttttgccg cgccggtgct ggtcgggatc atcgacatgc ctgccgcgcg cccgcgccgc
     6361 atgtccttgt ggggcagatc gagtaactgc tggtcgttcg tgtaggaatg gatcgtggtc
     6421 atgaggccgt gcttgatgcc gaaattatcc aacagcactt ttgccacagg agccagacag
     6481 ttggttgtgc aggaggcgtt ggaaataatg tggtgcttgt tcggatcgta ggccgcatcg
     6541 ttgacgccca ggacgatggt gacgtccggg tctttcgccg gtgcggaaat aatgacgtgt
     6601 ttggctccgg cagagaggtg cttccctgcg ccttcgcggt cggtaaaccg ccctgtcgat
     6661 tcgatgacaa tgtccacatc cagatccttc cagggaagtt cttttgggtc cttcatcgcg
     6721 agcaccttaa tgggcttgcc gtcgacgagg atctggtcgc ctttggcctc gacgcttccc
     6781 ggtaacgttc cgtgtacaga atcgtatttc aagagatagg cgagcgtctt cgcatccgtg
     6841 aggtcgttaa tcgcgacaaa atggagatca ggatccccga gtgatgcccg caggacgttc
     6901 cgtccaatcc gtccgaaccc gttaatgccg atccgaatag ccataataag tggtccctca
     6961 agaaaaaatg aaccgttgaa tccactggct tcaacgaagc cgatagtatc gacgcacctg
     7021 ggccttgtca aggcatacca aggtggtaat cccacgtcgg atcacagaaa tttcattgcc
     7081 acctgaatct gatgttgacg gagttcgtgc gaggtccgta gacctgtgcc ttgcgtctct
     7141 gtcgctgggt tcgttagagt tcgtcgattc cggcgtaaga ccatttttga ggtagcggtg
     7201 aatctcttag agcagaccac gatggtgttg gagtggccca gactgcttga ggcccttgca
     7261 gggcacgctc gttcgacgat gggggctgcg ggctgtcgtg ctcttgaatt gacaaccagt
     7321 ctccatgatg cacgacggcg tcagcaggaa acgacggaga tgggacagct gcaagcctcc
     7381 ggcgaggatc tgccgacctt ggcattcccg gatattcgtg acccgcttgc cagggcgaag
     7441 aaaggggcag cgttggaagc ccacgaactc cgggattgcg cggtagtgct gggcttggta
     7501 gaggagatcg gtcgctttgt gaggcggcat caacacgatg ctcccgcact ggcctccgtt
     7561 gcgaatcacc tgccaggggg cagggaatta cactcagtga aagcagcgtt ggatgcggcg
     7621 atccatcccg acggctcgat caaggagtct gcgacgccgg agttgcggcg ctcgacccag
     7681 caggcccagt cgctcaaaca gcagatgcgg aatgacctcg atcagatcct gcactcgcgt
     7741 cgctatcagg acatcctgca ggaactgtat tttgcccaac gagaaggccg gtatgtcatt
     7801 ccggtaaaag ccgacatgcg gggacgggtg ccggggatcg tccatgacat ctcggccagc
     7861 ggggctacgg tatttctcga accgcgcgaa ctggtcgaac tgaacaattc gatcaaagtg
     7921 gcggatcttg aaattgaacg ggaagtgcgg cgcattcttc gtgagttgtc cgccctcgtc
     7981 gccgcgcaat ccgaagtcgt gctggcaggg cttgaggcgt tggctgtact cgacggtatt
     8041 tcggcgcgtg catcattcag tcgccaacta aaagcgcatc ctgtcgggct caatgcagaa
     8101 gggcgcgtac ggctgttgcg cgccaggcat cccttgctcg tgctgtcgaa agaacaggtg
     8161 gtggcgaacg atattctttt gggtgaatcg atccaggtgc tcgtcatctc tggccccaat
     8221 gcggggggaa agaccgtgac tctcaagatt gttgggctct ttgccttgat ggtccgggcc
     8281 ggattgcacc tgccctgtga tgcgggatcc gagatggcgt tcttttctaa cgtctgcgcc
     8341 gatatcggcg atgcccaaga cctggctcgc gacctctcca gtttttcggc acacatgacc
     8401 cagatgattc agttgctgaa tgaaacggat tgccggcgac gagacgacga acatggggcc
     8461 ccgcgacagt ggttggtcct ccttgatgaa ccagtgacgt cgaccgatcc gacagaaggt
     8521 gcggcccttg ccgaagcgtt gctctgtcga ctggcgacgt tggggatgaa ggtcgtcgcg
     8581 accacgcatt atgggtcgct caaggccttg gcccacacga tgcccggctt cgccaacgcc
     8641 agcgtcgaat ttgacgtggc gacgctctcg ccgacctatc ggctcttcat gggggtgcca
     8701 ggcgggtcat ctgcgcttga gattgccggt cggctaggga tagaccgggc tttgctggat
     8761 gaggccagga agaagctcca taaagacgag cgggctatgg aaacgatgtt gcatgacctg
     8821 caagcgacgc agcggcggtt ggccgatgat ttggcgcggg ctgtcgaggc caggcgtgaa
     8881 gcggaacagg ccgaacaacg agcgaaggct cagctagccc atctggaaga gactgaacgt
     8941 ga
//
LOCUS       DATIPF010000418         6800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1673998, whole genome shotgun sequence.
ACCESSION   DATIPF010000418 DATIPF010000000
VERSION     DATIPF010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673998"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VLL06_10450"
     CDS             <1..202
                     /locus_tag="VLL06_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31427.1"
                     /translation="GTVVLDRVDRDSDEAHALFDESIPRPCIRGQLPVAVRSPISAVK
                     DDQDRSAGQMFSQVPGLAVLVR"
     gene            complement(304..723)
                     /locus_tag="VLL06_10455"
     CDS             complement(304..723)
                     /locus_tag="VLL06_10455"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSS31428.1"
                     /translation="MNPDDFPAPKDGFVITHFLVVSDQDRSRDFYRSIFDGLVLLERD
                     PVIMKVANTWLIMNVGGGPTDDKPTVTLATPPDPNRTSAFLNVRVANIQKAYKEWSAK
                     GAEFLTEPKDHGREIRAYMRDPDGHLIEVGQATGVLG"
     gene            complement(713..1018)
                     /locus_tag="VLL06_10460"
     CDS             complement(713..1018)
                     /locus_tag="VLL06_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HSS31429.1"
                     /translation="MVGRVMEFDLHAQPGIQVYTATRCGDCQMAKAVLDRAGADYEQI
                     DIDGDPAAADIVVAINGGYRTVPTIIFPDGRVLVEPRPSELLTALGQSTNLEVSDEP"
     gene            complement(1077..1415)
                     /locus_tag="VLL06_10465"
     CDS             complement(1077..1415)
                     /locus_tag="VLL06_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31430.1"
                     /translation="MSAAHIVEACAASALLALSFAALTFAIGAGTGSRSLAISVSSAL
                     AVAGFVIEGIAAQVPALRPIRELSPWHWMLGSDPLQDGFQWHWWLLPLVVSFLLVSLG
                     VARFRVRDLH"
     gene            complement(1424..2113)
                     /locus_tag="VLL06_10470"
     CDS             complement(1424..2113)
                     /locus_tag="VLL06_10470"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HSS31431.1"
                     /translation="MRTKTKTASRPLLLLFLFILIMAGVLVGFGVPLVSSEGMSIRAR
                     LIEGELPVEPEDAAWAKVAPMTLPLSGQVITRPVWPEPTARALTVRALHNGTEIAFLL
                     EWQDNTKNDRLTPGTFRDGVAIGLPLGDAPAFFCMGQLDHYINIWHWKADWQSDIDRR
                     AAKTSEKARDGVRTFEVIPRRVSSVEDLIGGGFSTLTTKEKQGRVQGKAAWSWPRFPR
                     PRFWRWRHQRV"
     gene            complement(2208..3461)
                     /locus_tag="VLL06_10475"
     CDS             complement(2208..3461)
                     /locus_tag="VLL06_10475"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31432.1"
                     /translation="METELQLATGYIETFAGNGKARSTGDGKRAVKSGIPLPHHAALD
                     KTETWFYFAESGSDRIRRVNLQEGTLHNFAGIGETCYSGDEGPCGEAGLYLPLDVAFD
                     SRNDLYLCDSGSNRIRKVDHETGIITTIIGTGQHGFNGDGPALEINLTWPAAIAFDSN
                     DVMYIADTQAHRIRRYDPRTGLVTTVAGTWTAEDEAREQPLVARNLVVLSGDAIGIDF
                     SDDHGWLMPVCSDGLDMSMYLDDGKPALEARLYDVVGLTVNGKGDIIFVDKGSNRVRS
                     IDHLNGIVSTVAGVCRYGYDGDDKPAIKAMLHAPEAVVIDRQDNLYISDTMNHRVRKV
                     DVTTGVITTVAGNGDSGYEDKNMGGCGAARFVAKESAGTVKHGDGLLGIEAVVNSPVG
                     LSLDSQGYLYICERGENKIRRVKLW"
     gene            complement(3487..3768)
                     /gene="tatA"
                     /locus_tag="VLL06_10480"
     CDS             complement(3487..3768)
                     /gene="tatA"
                     /locus_tag="VLL06_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HSS31433.1"
                     /translation="MFGSLGFTELILILFIVLIIFGAGKLPQLGEGVGKAIRGFKKSV
                     HEGDAIEAEAQAAQQQTAAAPQQPASAPLMQATAPVEQPAAPVHSGSRG"
     gene            complement(3797..4606)
                     /locus_tag="VLL06_10485"
     CDS             complement(3797..4606)
                     /locus_tag="VLL06_10485"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS31434.1"
                     /translation="MNEPSPSVPDQSQTIVDPGDQPLSPDEEIAEIEKLLATEPDDFQ
                     ARCRLGELYFSKGRMDDALVEVKKSIEMAEGLRAEMNRSLAMYYSNLGTIYATKAMTD
                     EAETEFKHALEVFPHDVLALFNLGRVYADKKKFMEAKEYYERLVEITPDDPMAWYNLA
                     GVYVELDNPEVTDYNTIDMAIQCYIRTLELDPKHLEGSFKLMELALNHKKTDLAIKVM
                     EEAVEQNPEEPLAYYNLISVYDKAKMFEQAEQTRKRLKERFSKRPKESSAS"
     gene            complement(4634..5758)
                     /locus_tag="VLL06_10490"
     CDS             complement(4634..5758)
                     /locus_tag="VLL06_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31435.1"
                     /translation="MIATSPIIDRITGKISDQSLLFQYTIKLVLIVSFSELILYRLVS
                     RLGMHLSKMAQEHEWIIPTFTALTEIGQWLLNVVAILLFLALGVAAVNRMAGRGFVGS
                     NMIVIPGVALLLLLTAGFLLIPPAFLGSAIYNLVALVVLICLMVEYLSTHTEWSQRAM
                     GITYLLGIGGWLYYQIVSTIYGFIGTVAAPPLVYEAHRGGEALLVLSSILVMWAYGRG
                     VSFWTKNHRQRRRAIWFWVTSVSTFTVMLFLDYLLHRYDAGMAGSIRKAADGIGWIFQ
                     FGMGYTFFMPFAIYMTGLLCWSYTVIKLLNMGRLAGYGLGLMFIAGYALLYSNLTLMV
                     VLGAMLLTMDRQRRDATDPEPATNRPMVSRPDSLVGGHVS"
     gene            complement(5835..>6800)
                     /locus_tag="VLL06_10495"
     CDS             complement(5835..>6800)
                     /locus_tag="VLL06_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSS31436.1"
                     /translation="VPNLIYRQNGKITVNQPFYSENINQLPAPNYDGFPLGLYLAPEP
                     VLPVQFSRGCYYKDCAFCALTLDHQNFRQKEPGRTIEELEWLKERYGAQRFFFTDECF
                     ALSPTKRLCQQIIEKKLDIKWTCEMRFEKHLTRELLSSMRDAGCLKIVFGLESFNQRV
                     MDLMQKGIKQEWVRRIADDCVDLGIAVHCYIIVGFPTEKEEEARETMNFIVENERLHG
                     SYGFSCQPCLFDLEKEAPIMSDPGGYGIQRIMRPSAEDLSLGFFYEVSEGMTPDEAER
                     LYQHVYEKVSEVVCELPFNYSMADGLLYIARAKAQEVGVPQSAGS"
BASE COUNT         1513 a         2016 c         1846 g         1425 t
ORIGIN      
        1 tggcacggtt gttctcgacc gggtcgaccg agattccgac gaggctcacg ccctctttga
       61 cgaatcgatc ccaaggcctt gcatacgagg ccaactgccc gttgcagtac ggtcaccaat
      121 ctccgcggta aaagacgatc aggaccgctc cgcgggccaa atgttcagcc aggtgccagg
      181 tctggccgtc ctggtcaggt agctcgaagt cgatcgcctt ttccatgttt accctctatc
      241 gcgtctttcg atctggtgtc gcaaggacta gtcttccaac cgacgcgctc caactcggtg
      301 gagtcagcca agcacgccag tcgcttggcc gacctcgatg agatgaccat caggatcacg
      361 catataggcg cgaatctccc gcccgtggtc cttcggctcc gttaggaact cggcgccctt
      421 ggccgaccat tccttgtaag ccttctggat gttcgcgacg cgaacgttga ggaacgcact
      481 ggtgcggttg gggtcgggcg gcgtcgccag ggtgaccgtt ggcttgtcgt cggtggggcc
      541 gccgccgacg ttcatgatca gccaggtgtt cgcgactttc atgatgaccg ggtcacgctc
      601 gagcagaacc aggccgtcga atatcgaacg atagaagtct cgagaccggt cctgatccga
      661 cacgacgaga aagtgtgtga tgacaaagcc gtctttgggc gcaggaaagt cgtcagggtt
      721 catcagacac ctccaggttt gtcgactgcc caagggcagt cagtagctcg ctcgggcgag
      781 gctcaaccag cactcgaccg tcaggaaaga tgatggtcgg gaccgtgcga tagccaccgt
      841 tgatcgcgac cacgatgtcc gcggccgctg ggtcgccgtc gatatcgatt tgctcgtaat
      901 ccgcaccggc acgatcaagg actgcttttg ccatctggca atctccgcat cgggtggcgg
      961 tgtacacctg gatgcccggt tgagcgtgca ggtcgaactc catcactcgt ccaaccattg
     1021 tgtgagcgcc cgctattccg gcagctcgtc tcggcaacac cgcgaaagag cccaggtcag
     1081 tggagatcgc ggaccctgaa tcgagcgact cccaatgaca ccaagagaaa gctgacgact
     1141 agaggcagga gccaccaatg ccattggaat ccgtcctgca agggatcgct gccgagcatc
     1201 cagtgccacg gcgatagttc acgaatcggg cgcagagcgg gtacctgcgc ggcgatgccc
     1261 tcgatcacga aaccagccac ggccagagcc gaagaaacag agattgccaa agatcggctg
     1321 cccgtccctg cgccgattgc aaacgtaagc gcagcgaagc tgagggcgag cagggcgctc
     1381 gcggcgcacg cctcgacaat gtgggcggcg ctcacgccgt tggtcagacc cgttgatggc
     1441 gccagcgcca gaagcgtggc cgcggaaacc gaggccagga ccacgccgct ttcccctgaa
     1501 cacggccctg cttctctttg gtcgtcagag tactaaagcc tccgccgatg aggtcttcga
     1561 cggatgaaac ccggcgcgga atgacttcaa acgtccgcac gccatcccga gctttttcag
     1621 aagtcttggc cgcccggcga tcgatatcgc tctgccaatc ggccttccaa tgccagatat
     1681 tgatgtagtg atcgagttgg cccatacaga agaacgcggg agcatcaccg agaggcagtc
     1741 ctatcgccac gccgtcgcga aaagttcccg gcgtcaggcg atcgtttttt gtgttgtcct
     1801 gccattcgag cagaaaggcg atctctgtgc cgttgtgaag ggcccgaacc gttaaggctc
     1861 gagcggtcgg ctcaggccag accggcctgg taataacctg accactcaat ggaagggtca
     1921 tcggggcaac cttggcccag gcagcatcct ccggctcaac cggcaattcc ccttcgatga
     1981 ggcgggcacg aatgctcatc ccctcggaac tgacgagggg cacgccgaag cccactaaga
     2041 caccggccat aatcagaatg aagaggaaca gaaggagtaa cgggcgggaa gcagtcttcg
     2101 tcttcgtacg cactatgcgg gccctcgagt gaccggcgcg agcagatctt cgttgattta
     2161 gactaccaca aagcccgggc gagaccaatc cgactgcctc aggatttcta ccagagtttc
     2221 acacggcgaa ttttattttc gccccgctcg cagatgtaga gatagccctg ggagtccaat
     2281 gagagtccga ctggagagtt gacaacggcc tctattccga ggagcccgtc tccatgtttc
     2341 accgtccctg ccgattcctt cgccacgaag cgcgcagcgc cgcagccacc catattcttg
     2401 tcctcgtagc cgctgtcccc attgcccgcc accgtcgtaa tcacaccggt cgtgacatcc
     2461 accttgcgca cgcggtggtt catcgtgtcg gagatgtaaa gattgtcttg ccgatcgatc
     2521 accacagctt ccggcgcatg gagcatggct ttgatcgccg gcttgtcatc gccgtcgtaa
     2581 ccataccggc acacgcccgc cacggtagag acgatgccgt tgaggtgatc gatgctgcgt
     2641 acgcggttac tgcccttgtc gacaaaaata atgtcgccct tcccgttcac cgtgagcccg
     2701 acaacatcat agaggcgggc ttcgagcgca ggtttcccat cgtcaagata catggacatg
     2761 tccagtccat ccgaacatac cggcatgagc caaccatggt catcgctgaa atcgataccg
     2821 atggcatcgc cggagagcac gaccaaattc cgcgcaacga gcggctgttc acgggcctcg
     2881 tcttcggctg tccatgttcc ggccacggtc gtcaccaagc cggttcgggg atcgtagcgc
     2941 cggattcggt gtgcctgtgt gtcggcgatg tacatcacat cgttggagtc aaaggcgatg
     3001 gcggcgggcc aggtcagatt gatctcgagg gccggaccgt ctccgttaaa cccatgctga
     3061 ccggtgccga tgatcgtggt gatgatgccg gtctcatggt ccacttttcg aatgcggtta
     3121 ctgccggaat cgcagagata gaggtcgttg cgggagtcaa atgcgacgtc gagcggcaaa
     3181 tagagcccgg cttcaccaca gggtccctca tcgccgctat agcaggtttc gccgatgccg
     3241 gcgaaattgt ggagtgtccc ttcttgaaga ttgacccgtc ggatacgatc ggacccggac
     3301 tcggcaaagt agaaccaggt ctcggtcttg tcgagtgcgg cgtggtgagg cagaggaatc
     3361 ccggacttga ccgcccgttt tccatcgcca gtgctccggg ccttgccgtt acctgcgaag
     3421 gtttcgatgt agccggtcgc aagctgaagt tcggtttcca tctgcctgtg accttatccc
     3481 ggctggttag ccgcgtgaac ctgaatgaac cggtgcggcc ggctgttcta ctggggctgt
     3541 tgcttgcatc aagggcgcag aagctggttg ctgcggagcg gctgccgtct gctgctgagc
     3601 cgcttgagct tcggcttcga tcgcgtcccc ctcatgcacg gacttcttga agcccctgat
     3661 cgccttgccg accccttctc ccagctgcgg caatttgccc gctccgaaga tgatcagcac
     3721 aatgaaaagg atcaggatca attctgtaaa accaagactt ccaaacatgg tgtgtccctc
     3781 cgtacgaatg ggtcgatcag gatgcgctgc tctccttagg tcgtttggag aatcgttcct
     3841 tcaaccgctt ccgtgtctgt tcggcctgtt caaacatctt tgccttgtcg tagacactga
     3901 ttaagttgta gtaggccagc ggctcctcgg gattctgctc gacggcctct tccatgacct
     3961 tgatggcgag atccgtcttc ttgtgattca gcgccaactc catcagcttg aagctgccct
     4021 ccagatgctt cgggtccaac tcgagcgtac ggatatagca ttggatggcc atgtcgatcg
     4081 tattgtagtc cgtgacctct ggattatcga gttcgacata gactcccgca agattatacc
     4141 aagccatcgg atcatccggt gtgatttcca cgaggcgttc gtaatactcc ttggcttcca
     4201 tgaatttctt cttgtcggca tagacccgtc caagattgaa gagcgcgagc acgtcgtgag
     4261 gaaacacctc aagggcatgc ttgaactcgg tttccgcctc gtccgtcatg gccttcgtcg
     4321 cgtagatcgt acccaaattg gaatagtaca tcgcgaggga ccgattcatt tctgcacgaa
     4381 gcccctccgc catctcaatc gacttcttga cctccacgag cgcgtcatcc atccggcctt
     4441 tgctgaaata gagttcgccc aatcggcagc gggcctggaa gtcgtcgggc tcggttgcca
     4501 agagcttttc aatctcggcg atttcctcgt cagggctcag cggttgatca ccgggatcga
     4561 cgatcgtctg gctttggtca ggaacagagg ggctcggttc gttcatacaa tcaattccct
     4621 atcgtaaaaa ggcctatgag acatgtccgc ccaccaaaga atcaggtctg ctgaccatgg
     4681 gacgattcgt cgccggttcc ggatccgtcg catcgcgccg ttgccgatcc atggtcaaaa
     4741 gcatcgcacc gagcactacc atgagcgtca gattcgaata caaaagcgca tacccagcaa
     4801 taaacatcaa tccaagccca tacccggcga gtcgacccat gttaagtaac ttaatcaccg
     4861 tgtacgacca gcacaacaaa ccggtcatat agatcgcaaa cggcatgaaa aacgtgtacc
     4921 ccatgccgaa ttgaaaaatc cacccgatgc catctgccgc cttacgaata ctaccggcca
     4981 tccctgcgtc ataccgatgg agcagataat ccaaaaacag catcaccgta aacgtggaca
     5041 ccgaggtgac ccaaaaccag atggcgcgcc ggcgttgccg atgattcttc gtccaaaatg
     5101 agacgccacg tccataggcc cacatgacca ggatgctcga caagaccagt agcgcttcac
     5161 ccccgcgatg ggcttcatag acgagtggcg gggccgcgac agttccgata aagccataaa
     5221 tagtggatac gatctgataa tacaaccagc ccccgatccc aagaagatag gtgatgccca
     5281 tggcacgctg cgaccattcg gtgtgagtag agaggtattc caccatcaga cagatcaaca
     5341 cgaccaaggc gacaagattg taaatggcag atcccaggaa agcaggcgga atcaacaaaa
     5401 accccgccgt caagagcaag aggagcgcga caccgggaat gacgatcata ttcgatccca
     5461 caaacccacg accagccatt cgattgacgg ctgccacacc cagggcaaga aagagcaaaa
     5521 tcgccacgac attgagcagc cactgcccga tctccgtcag cgccgtgaag gtcgggataa
     5581 tccactcatg ttcctgcgcc atcttgctga gatgcatccc taagcgcgag acgagccgat
     5641 agaggatcaa ttccgagaag gagacgatca ggacgagctt gatcgtgtat tgaaatagaa
     5701 gggattgatc gctgatcttc cccgtaatcc ggtcgatgat ggggctcgtg gcaatcactc
     5761 tatgtaccct tgaggaatct ggaaattata ccgatcgaga aaaaacaggg tcaacgtcga
     5821 gcagaacggg ctcttcacga cccagccgac tgcggcacgc ccacttcctg ggccttggcc
     5881 cgtgcaatat acagtaatcc atcggccatc gaatagttga acggcaactc gcacaccact
     5941 tcgctgacct tttcgtacac atgctggtat aggcgctccg cctcgtccgg cgtcatgcct
     6001 tccgagactt cataaaagaa acccaggctc aagtcttctg ccgacgggcg catgatacgc
     6061 tgaatgccat agcctccggg gtcgctcatg atcggtgctt ctttttccaa atcgaagaga
     6121 cagggctgac aagagaaccc gtacgatccg tgaagccgtt cgttctccac aataaaattc
     6181 atggtctccc gggcttcttc ttctttctcg gtcgggaatc ccacaataat gtagcagtgc
     6241 acggcgatcc ccaagtcgac acaatcgtcg gcaatgcgcc gcacccattc ctgcttaatc
     6301 cctttctgca tcaagtccat tacgcgctga ttgaaggact ccaatccgaa tacgatcttc
     6361 agacacccgg catcccgcat cgacgagaga agttcgcggg tcagatgctt ctcgaatcgc
     6421 atctcacagg tccatttgat atccagcttt ttctcgatga tctgctggca caaccgtttg
     6481 gtcggcgata aggcaaagca ttcgtcggta aagaaaaagc gttgagcccc atatcgctcc
     6541 ttgagccatt ccaattcctc aatggtccgt ccaggttctt tctgtctgaa gttctggtga
     6601 tccagggtga gggcacagaa ggcacaatcc ttgtagtagc accctcgtga gaactgcacc
     6661 ggcaacaccg gctccggcgc cagatacagt ccgaggggga agccgtcgta gttgggggcc
     6721 ggcagctggt taatgttttc agaataaaac ggctggttaa ccgtaatctt cccgttctga
     6781 cgatagatca ggttcgggac
//
LOCUS       DATIPF010000419         4988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_522776, whole genome shotgun sequence.
ACCESSION   DATIPF010000419 DATIPF010000000
VERSION     DATIPF010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522776"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VLL06_10500"
     CDS             complement(<1..763)
                     /locus_tag="VLL06_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSS31437.1"
                     /translation="MPVYHYKGYRNDGGAATGIIDAESPKVARVKLRKVGVFPTDMVE
                     QDAAPTVSTKTGIPGRPSVGIGRSPALSTTDVAMMTRQLATLLVAGLPLVDALGVLVD
                     QSEKKSVKSLMADIREAIRGGTAYSAVLGGYPREFSQIYVHMVRAGEASGALDQILFR
                     LAEFLEKQLALKHKVTNAILYPALMMIVGISVLFFLMTFVVPKITAVFTSLKQALPLP
                     TVILMSISHFLADYWAVILGGVLLVVWGTRRAMKTE"
     gene            complement(767..2494)
                     /gene="gspE"
                     /locus_tag="VLL06_10505"
     CDS             complement(767..2494)
                     /gene="gspE"
                     /locus_tag="VLL06_10505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HSS31438.1"
                     /translation="MNDEHSGRPLLGTILEDKFGLTEAKLLEAVNLQQTKGGRLGEIL
                     IRLRAITEEQLLQALAVQFELPWLPDLDVNQVDHEWVKKVPIHFARRYHVLPLKTEDG
                     AVLVATTDPMETAALDDLRLLLGLPIKPILTSSLSLLACLNRVYDEAASPAGAEQVME
                     DIAASESLDQIAHELDEPQDLLDATDEAPIIRLVNSVLFQAVRQRASDIHFESFERGL
                     VVRYRIDGVLYPVLTPPKHLQASIIARLKIMAALNIAEKRLPQDGRFRIRTAGKDVDL
                     RVSVLPTSHGERVVLRLLEKENRLLNLSEMGFSQDRLGIIQQLIQLSHGIILVTGPTG
                     SGKTTTLYAALTQINAPDKNIITVEDPVEYQLLGVGQMQVNPKINLTFAAGLRSILRQ
                     DPDVIMIGEIRDRETAEIAIHASLTGHLVFSTLHTNDAASAATRLIDMGIEPFLVASS
                     VVAVLAQRLLRQVCSDCKQPYPPADEELIRLGIVPPRVRPTFYRGVGCPACSQTGYRG
                     RTGIFELLVLDEEIRRLIGNKADSASIRQAAMAKGMVTLKDEGAEKVFHGVTTTEELM
                     RITQQEVEI"
     gene            complement(2724..3596)
                     /locus_tag="VLL06_10510"
     CDS             complement(2724..3596)
                     /locus_tag="VLL06_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31439.1"
                     /translation="MNIRRAIIFAYVICATFLVAHIINAVIAEALSVPAGLVRPSPAS
                     EQTSEPSASVPALVEQIQASRLFPLPIDPLGISTTGPGSGSLPSRAPLNLASKLTLLG
                     VVMGDLGGVSVIVEELSSKRQLFFRLHDHIPDVGEISEIRRDGMVVRLGDQQEFLGLA
                     ASQMEKPVMAPVPVGSAVVPVPGSPIRKVLDRREVEQAMGDLPKLLTQARAVPYLVNG
                     AINGFRLDFIAPSSFYEKIGLKYGDVLQQVNGVEIRDPGTMLTLLQQLRNEKTVKLDV
                     LRNNQRTAMTYDIR"
     gene            3778..4791
                     /gene="gmd"
                     /locus_tag="VLL06_10515"
     CDS             3778..4791
                     /gene="gmd"
                     /locus_tag="VLL06_10515"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HSS31440.1"
                     /translation="MRKALISGITGQDGSYLAEFLLGKRYEVYGIIRRSSSFNTGRID
                     PIYEDPHVPHRRLHLVYGDLNDASSLNRIIRMIQPDEIYNLGAQSHVRVSFDIPEYTG
                     EITGLGTIRLLEAIRESGLKPKFYQASSSEMFGKVQDVPQRETTPFYPRSPYGAAKVY
                     SYWVTVNYREAYDLFACNGILFNHESPRRGETFVTRKITKAAARIKLGVQQDLFLGNL
                     DAKRDWGFAGDYVEAMWMMLQAKTPDDYVIATGETHTVKEMLELAFDRVQLDWKKHVK
                     IDQKYYRPTEVDLLIGDASKAKKELGWEPKVRFHELIAMMVDADLAAEKEKLDGTRKK
                     VSG"
BASE COUNT         1177 a         1375 c         1333 g         1103 t
ORIGIN      
        1 cctctgtctt catggcccga cgtgttcccc agacaaccag cagcaccccg ccaagaatca
       61 ccgcccaata atctgcgaga aaatggctga tactcatcag gatgaccgtc ggcaagggga
      121 gggcctgctt taaactcgta aagactgcag tgattttcgg caccacaaag gtcataagga
      181 aaaatagtac ggagattccg acgatcatca tcaaggcggg atagagaata gcattcgtca
      241 ccttgtgctt gagggccaac tgcttttcca gaaactctgc gaggcggaat aggatctggt
      301 ccaacgcgcc gctggcttca ccggcccgta ccatatgcac gtaaatctga gagaactcgc
      361 gcggataccc ccccagtacc gcactgtagg ctgtacctcc ccggatggct tcgcgaatat
      421 cggccatcag gctcttcacg gattttttct cggattgatc caccaagacc ccgagtgcgt
      481 cgaccagcgg tagaccggcc accagcaatg tcgctagctg tcgtgtcatc atcgccacat
      541 cggtcgtgct gagggcaggg gagcggccga tcccaacgga gggtctgcca ggaataccag
      601 tcttcgtcga aacggttggg gcagcgtcct gttccaccat atcggtggga aatacgccga
      661 ccttgcgcaa ttttacgcgc gcaacctttg gactctcagc atcgatgata ccggttgcgg
      721 cccctccatc gtttcgatag cccttataat ggtagacggg catcttctag atctcgactt
      781 cctgttgggt aatccgcatc aattcttccg tggtcgtcac cccgtggaac accttctctg
      841 ccccttcatc tttcaacgtc accatgccct ttgccatcgc cgcctgacga atcgacgccg
      901 aatccgcctt gttaccgatg aggcggcgaa tctcttcgtc caacacgagt agttcaaata
      961 tgcctgttcg acctcgatag ccggtttgtg aacaggccgg gcacccgact ccccgataga
     1021 atgtggggcg taccctcggt ggtacaatcc ccagccgaat cagttcttca tcggccgggg
     1081 gatagggctg cttacaatcc gaacagacct gccgcagcaa ccgttgtgcg aggacagcga
     1141 ccaccgacga ggcgacgaga aacggctcga tgcccatatc aatcagtcgt gtggccgcac
     1201 tcgccgcatc gttcgtatgg agcgtggaga agaccaaatg tcccgtaagt gaggcatgaa
     1261 tggcgatttc ggcggtttca cgatcgcgga tttcgccgat catgatgaca tcgggatctt
     1321 gcctgagaat cgaccgtagc cccgctgcaa aagtcaggtt gatcttgggg ttcacttgca
     1381 tctgacccac accgagcagc tgatattcga ccggatcttc gacggtaatg atgttcttgt
     1441 ccggtgcatt gatctgcgtc aatgctgcat agagtgtcgt cgtcttcccg ctcccggtcg
     1501 gcccggtcac gagaatgatg ccatgtgaca actggatcag ttgctgaatg attcccaatc
     1561 ggtcttgcga aaaccccatt tctgacagat tcaacagacg gttctctttt tctaatagac
     1621 gcaggacgac ccgttcgcca tgtgaggtcg gcagcacaga gacacgcaga tcgacatcct
     1681 tccccgccgt ccgaattcga aagcggccat cctgcggcaa gcgtttttcc gcgatgttca
     1741 aagcggccat aattttcaaa cgcgcgataa tactcgcctg taaatgtttc gggggggtta
     1801 agaccggata gagcaccccg tcgatgcgat accgaaccac cagcccccgt tcgaacgact
     1861 caaaatgaat atcgctcgct cgctgcctga ccgcttggaa gagcacggag ttgacgagac
     1921 gaatgatcgg cgcttcgtcc gtggcatcga ggaggtcttg cggttcatcc aattcgtgag
     1981 cgatttgatc caagctctca ctcgccgcga tatcctccat cacttgctcg gcccccgcag
     2041 ggcttgcggc ttcgtcatag acccgattca gacaggccag aagcgacaga ctgcttgtga
     2101 gtatcggttt gatcggaagc ccgagcagga gtcgaagatc gtccaatgca gctgtttcca
     2161 tgggatcagt cgtcgccaca agcactgctc catcctctgt cttgagcggg agcacgtgat
     2221 agcgccgagc gaagtggatt ggaactttct tcacccattc gtggtcgacc tggttcacat
     2281 cgagatccgg aagccaaggc agttcaaatt gaacggcaag tgcctggaga agttgctctt
     2341 cggtgatggc tctgagtcgg atcaagattt cgccgagacg ccccccctta gtctgctgga
     2401 ggttgactgc ttccagcaat ttggcttcgg tcaatccaaa tttatcttcg aggattgttc
     2461 ctaagagcgg ccgaccagaa tgttcgtcat tcatcacaca acacccagcc ttgaagggtt
     2521 catcacgtcg cgcagttcat caggttcggg ataaggccac aaccctttgg agtttaagag
     2581 aatactctct accagttctc tctgacaaga tcatgaaggc tcctagccct tctgaggggc
     2641 atgccttcct catcctgccc acctgaggcg ctggagatac cgccagctgt tgaaaacaga
     2701 aaagggcata aaccgaggag gattcagcgg atgtcgtatg tcatcgcggt tcgctgattg
     2761 ttacgcagga catcaagctt cacggtcttc tcattgcgga gctgctggag gagggtgagc
     2821 atcgtacctg gatcacgaat ctccacgcca ttgacctgct ggaggacatc cccatacttc
     2881 aagccaattt tctcataaaa gctcgagggc gcgatgaagt cgagacggaa cccattgatc
     2941 gctccattga ccaggtaggg tacggctctc gcctgagtca ataacttggg tagatcgccc
     3001 attgcttgtt caacctctct ccgatcaagc actttccgta tagggctccc aggtaccggg
     3061 acgacggctg atcccaccgg tactggggcc atcacgggct tctccatctg cgaggcggcg
     3121 agtccaagaa attcctgttg atcacccagc cgaacgacca tcccatctcg acggatttcg
     3181 ctgatctcac ccacatccgg tatgtggtcg tgcaatcgga aaaatagttg gcgtttgctg
     3241 gagagttctt ccacgatgac cgacactccc ccaaggtcac ccatcaccac tcccagcagc
     3301 gtgagtttcg atgcgagatt taacggggcc ctggagggca gcgaaccgga gccgggccca
     3361 gtcgtgctga taccgagggg atcaatcggc aggggaaaga gtctgctcgc ttgaatctgc
     3421 tcgaccaacg ctggtactga ggcggacggt tcactcgttt gctcggaagc cggtgacggc
     3481 cgaaccagcc ctgccgggac agacaatgcc tctgcaatca ccgcgttgat gatatgggcc
     3541 acaagaaacg tggcgcatat gacataggcg aaaatgatgg cccgacgaat attcatagtc
     3601 tgcgagaccg ggtccgggtc attcgtccca gtgagagaga ttctagtgcg agcgaagatc
     3661 acatgcaaga acaaccaatt gatcagcaga atgagcaaaa ccaaccagtc caatggacaa
     3721 cagatcgaaa tcaagacata attcgaccac ttccaacacg acctatggag tagtcttgtg
     3781 agaaaagcgt tgatcagtgg tattacaggg caggatggat cgtatttggc agagtttctc
     3841 ctcgggaagc gttatgaggt gtacggcatt attcgacgat cgagttcgtt caatacggga
     3901 cggatcgatc ccatttatga agacccccac gttccgcatc gacggctcca tctcgtctat
     3961 ggcgacctca acgacgcgag ttcactgaac cgtattatcc gaatgatcca gcccgatgaa
     4021 atttacaatc tgggagcaca aagtcacgtg cgcgtgagtt tcgatattcc cgagtatacc
     4081 ggcgaaatta caggacttgg aacaatccgc ctcctggagg ccattcgtga gtccggcttg
     4141 aagccaaaat tttatcaagc ctcgtcgagt gagatgttcg gtaaagtaca ggacgtccct
     4201 cagcgggaga ccacaccgtt ctatccccgc agtccctatg gagcagccaa ggtctattcg
     4261 tattgggtga cggtcaatta tcgagaagcc tacgatctct ttgcgtgtaa tggcatttta
     4321 ttcaaccatg aatcacccag gcgcggcgag acgttcgtga ctcgcaagat cacaaaagcg
     4381 gccgcccgca tcaagctggg tgtccaacaa gacctgttcc tgggaaatct cgacgccaaa
     4441 cgcgattggg ggttcgccgg cgactatgtt gaggccatgt ggatgatgtt acaggcgaag
     4501 acccccgacg attacgtcat tgccaccggc gaaacgcata ccgtcaagga aatgctcgaa
     4561 ctcgcattcg atcgtgtgca actagactgg aagaagcatg tgaagatcga tcagaaatac
     4621 tatcgcccga ccgaggtgga tctcctcatc ggggatgcga gtaaagcgaa gaaagaactt
     4681 ggctgggaac caaaagtgcg attccacgag ttgatcgcga tgatggtcga cgcggacctg
     4741 gctgccgaaa aagaaaaatt ggatggtact aggaaaaagg tttcaggtta gaagttggag
     4801 gcacgcgcgt tgctatgacg tcattttgga aggacaagcg tattgttgtc acgggcgggg
     4861 caggctttct gggatcgttc gtcgtggaac agctccgcgc aaagggctgc cgagagatct
     4921 ttgtgccccg cagcaaagac tacgaccttg ttcagataga tgctgtgaaa cagctctatc
     4981 gcgatcgt
//
LOCUS       DATIPF010000420         1970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_965055, whole genome shotgun sequence.
ACCESSION   DATIPF010000420 DATIPF010000000
VERSION     DATIPF010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965055"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VLL06_10520"
     CDS             <1..802
                     /locus_tag="VLL06_10520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSS31441.1"
                     /translation="MMTFSHLDAKSALVAGLVQELEQDGWIAQRRVIDVSPHIPLKGQ
                     TWESYLAGLGSQHRYNFNRRLKNLHKLGTLTFGVVENEEQRPEALRNLFDLHNLRWDQ
                     RGGSETLRSPTMQAFHDAFSRIALERGWLRLFILRLDGRPIGALYGFLYGRRFYFYQS
                     GFDPAYRQQSVGLVTMGLAIKHALAEGVEDYDLLHGTETYKALWASESRELVRIQMFP
                     PSIGGILCRAAVALDGRAKSAARRLLPQAMLDRAIAWKRGAYVADGGH"
     gene            914..>1970
                     /locus_tag="VLL06_10525"
     CDS             914..>1970
                     /locus_tag="VLL06_10525"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HSS31442.1"
                     /translation="MKNGVAAALGWTGTDRAVASWTGTKALPLVLAYHGVVEDIHARP
                     GLAKNPNLISLKMLECQLEWIGRRYRFISLDELGAQVERGNPFGQPSAAVTFDDGYAG
                     VYHLALPLLIQKGIPAGMFIVTDATGSPDLQIYDKTYLLLEKVLPLFGHSEERFHRLL
                     GANDIHIGAYNDGVRLDDAFRMMRWLFTTLPQKTLRRVIEALETVCRIDPDNYPEMQT
                     LDWEMVRIMADSGMTIGSHTQTHALLTEESAEQVGSQTVGSKLTLRDKLNRSIAHFAY
                     PDGRFNAAVVSAVAEAGYRFGYGTCLSRDRRHPGLTIPRTLLWERSCADGAGQFSAAL
                     MSCHANRLYDLWSPCAHD"
BASE COUNT          405 a          563 c          568 g          434 t
ORIGIN      
        1 gatgatgacc ttcagtcacc tcgacgcaaa gagcgcgctc gttgccggat tggtgcagga
       61 actcgagcaa gatggatgga tcgcgcagcg tcgtgtcatc gatgtgtctc cccatattcc
      121 gttaaaggga caaacgtggg aatcgtacct tgctggtttg ggatcgcagc atcggtacaa
      181 tttcaaccga cgcctgaaga accttcataa actcggtacc ttgacgttcg gcgtggtgga
      241 gaacgaggag caacggccgg aggctctgcg gaatctattc gaccttcaca atttgcgctg
      301 ggatcaacga ggaggttccg agacattacg ctcgccgacc atgcaagcct ttcacgacgc
      361 gttcagccgt atcgctctcg aacgaggctg gcttcgcctg ttcatcctca ggctggacgg
      421 ccgtcccatc ggggcgctct acggatttct ctacggtcgc cgcttttact tctatcagtc
      481 cggattcgat cccgcctatc ggcagcagag cgtgggtttg gtcaccatgg ggttggcaat
      541 caagcacgcg ctggcagaag gagttgaaga ctacgatctg ttgcatggaa cagagacata
      601 caaagcgctc tgggccagcg aatcgcgcga gttggtccgg atacagatgt ttccgccttc
      661 catcggaggc attctgtgcc gggcggcagt ggcactcgat ggtcgagcca aatcagctgc
      721 ccgccggctg cttccccaag ccatgttgga ccgagctatt gcctggaaac gcggcgctta
      781 cgttgccgat ggcggccatt agcggagacc tcatcagttc gtctcagcga ttgagcgtct
      841 agcggcccag aaccctcctt ggtccattca tcaccaatca ccatttctcc ccatccgcat
      901 gttgaaaact cttatgaaaa acggggtggc cgctgcgttg ggctggacgg gcactgaccg
      961 tgccgtcgcg tcctggacgg gaaccaaggc cttgccgctt gttctcgcct atcacggggt
     1021 cgtagaagac attcacgctc ggcccgggct cgcaaaaaat ccgaatttga tcagcctgaa
     1081 gatgttggag tgtcagttgg agtggatcgg gcgacggtac cgcttcatca gcttggacga
     1141 acttggcgcc caggtggagc gtggcaatcc gttcgggcag ccctcggcgg cagtgacctt
     1201 cgacgacggc tatgccgggg tctatcacct agccctgccg ttattgatac aaaaaggcat
     1261 accggcaggg atgttcatcg tcaccgacgc tacaggcagt ccggaccttc agatctacga
     1321 caaaacgtat ctcctacttg agaaagttct tcctctcttt ggccattccg aagaacgatt
     1381 tcaccgtttg ctcggcgcga acgatatcca tatcggcgcc tataacgacg gcgtacggct
     1441 cgacgatgcc ttccgcatga tgcggtggct gttcaccacc cttccccaga agaccttgcg
     1501 gcgtgtgatc gaggcactgg aaaccgtatg ccgaatcgac cccgataatt atccggagat
     1561 gcaaaccttg gactgggaga tggtacggat catggccgat tctggcatga ccatcggatc
     1621 acatacgcaa acgcatgcgc ttctcacgga agaaagtgcc gagcaggtgg gctcccagac
     1681 agtgggatcg aagttgacgc tgcgcgacaa gctgaatcga tctatcgcac attttgcata
     1741 tccggatgga cgtttcaatg cggctgtggt gtcggcggtt gccgaggcgg gatatcgctt
     1801 tggctacgga acctgcctca gccgcgaccg gcggcatccc ggcctgacca ttccccgtac
     1861 gttattgtgg gagcggtcat gtgcagatgg agccggacag ttttcagcgg ccctcatgag
     1921 ttgccacgcg aaccgtctgt atgacctctg gtctccctgt gcgcatgacc
//
LOCUS       DATIPF010000421         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1232163, whole genome shotgun sequence.
ACCESSION   DATIPF010000421 DATIPF010000000
VERSION     DATIPF010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232163"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VLL06_10530"
     CDS             <1..503
                     /locus_tag="VLL06_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HSS31443.1"
                     /translation="SILSHGKSSRLYQSLVYEQKSSLAVGAEYSLLQTDPGLFYFYAL
                     VSPGQKPEMVEDALHREIKRLQTDPPTDQELQRAKNQVEATHIFEQDSNFRRAMLLGQ
                     AESVGAGWRKIDQFVERIRAVTTKDIQRVARQYLTEDNRTVGTLIPVPPKQPEAPSAA
                     AQQVGP"
     gene            507..1874
                     /locus_tag="VLL06_10535"
     CDS             507..1874
                     /locus_tag="VLL06_10535"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSS31444.1"
                     /translation="MPTRRPLSLTLRRQVILTFVLGCCAFLLGSGPGVAADIAPTKFV
                     TANGITVLVLEQHFLPIVEIHALVKTGSAQDPPDKAGLANLVASLLDEGTTTRTSKQL
                     AEQIDFVGGALEAKASEDFTTASARVLKKDIDLGFTLLADMLQHPAFSKQEFERIRIQ
                     LLGEMASDNDDPGQIAMKAFNQLVFHGHPYRWPVNGSEDTLHKITLADVQAFYGREYS
                     PSQVILTIVGDITLEQATALTQTHFGAWKKSAATTRNMKPPSPIERRTVQLIEKDLTQ
                     STIVLGHGGLPRTHPDFYSVTVMNYILGAGGFSSRLMDSIRDKQGLAYGIMSHFDARL
                     MPGSFWVNLQTKTETTNQAITGVLSEIKSIRDTPVSDQELAEAKSYLIGSFPLRFDST
                     AKLAKVLAQVEFYGLGFEYFSDYQKWIDRVTKEDVQRVAKQYLDPKRYALVVVGNIAK
                     AKVKH"
     gene            2115..>2476
                     /locus_tag="VLL06_10540"
     CDS             2115..>2476
                     /locus_tag="VLL06_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31445.1"
                     /translation="MDQGAELDALIARMIEDYVSRNRAAKVLRDSLDKAGVGFYPVAD
                     HLTLRTMNIDRCAEEYTKLGYGYSETIEYEDWYAKVYRKVGYPALFVDQAYADDRGRT
                     SIIPGWVKTFGDQVYHHVA"
BASE COUNT          583 a          711 c          673 g          509 t
ORIGIN      
        1 attcgattct gtctcatgga aagagttcgc gcctgtatca gagcctcgtg tacgagcaaa
       61 agtcgtcgct ggcagtcgga gcagaataca gcttgctgca gaccgatcca ggccttttct
      121 acttctatgc cctcgtgagc cccggccaga aaccggagat ggtcgaagat gcccttcatc
      181 gagagatcaa acgtctccag accgatccgc cgacggacca agaactccaa cgggcaaaga
      241 accaggtcga agcaactcat atcttcgagc aggactccaa cttccggcgt gcgatgttgc
      301 taggacaggc tgaatccgtc ggtgctggct ggcggaagat cgatcagttt gtcgaacgga
      361 ttcgagcagt gacgaccaaa gacattcagc gtgtcgctcg ccaatacttg acggaagaca
      421 atcggaccgt tggcacgttg attcccgtgc cacccaaaca gccggaagcg ccctctgctg
      481 ccgcacaaca ggtagggccc taaattatgc ccacaaggag gccactgtct cttacgctcc
      541 gacgacaggt gatcctgacc tttgtgctgg gatgctgtgc cttcctttta ggaagcggtc
      601 ccggcgtggc ggcagacatc gcaccaacca aatttgtgac cgcaaacggc atcacggtgc
      661 tcgtccttga gcaacatttc ctcccgatcg ttgagatcca cgcgttggtc aagaccggct
      721 ctgcacaaga tcctccagac aaagcaggac tcgcgaacct cgtggccagt ctgctggacg
      781 aaggcacgac cacgcgtacg tcaaaacagt tggcggagca aatcgatttc gtcgggggag
      841 ctttggaagc caaagcctca gaggatttta cgactgcctc cgcccgcgta ctcaagaaag
      901 acatcgacct cggatttacc ctgctcgcgg acatgctgca acaccccgca ttctctaagc
      961 aagagttcga acgaattcgc atccaactcc tgggagaaat ggcgagcgac aacgacgacc
     1021 ctggccaaat cgctatgaaa gcattcaatc agctcgtctt ccatgggcac ccctaccgct
     1081 ggcccgtcaa tggatcggag gacaccctcc acaagatcac gctggccgac gtgcaggcct
     1141 tctatggacg ggagtattcc ccttcccagg tcatactgac aattgtcggc gatatcacgc
     1201 ttgagcaagc cactgcgctg acacagaccc attttggagc ctggaaaaaa agtgccgcga
     1261 caacacggaa catgaaacct ccctcaccga tcgaacggag aactgtccaa ctcatcgaga
     1321 aagatctcac ccaatccacc attgtccttg gtcatggggg ccttccgcgc actcacccag
     1381 atttctattc agtcacggtg atgaattaca tcctgggggc tggtgggttt tcctctcgcc
     1441 tcatggattc gattcgcgac aagcaggggc tggcctatgg gatcatgagc cacttcgatg
     1501 cccggctgat gcccggttca ttctgggtca atctccaaac caagacggag acgacgaatc
     1561 aagccatcac cggcgtcctg tccgaaatca aatccattcg tgacaccccc gtgtccgacc
     1621 aggaattggc tgaggccaaa tcctacttga tcggcagctt cccactacgg tttgattcca
     1681 ccgcaaaact tgcgaaagtg cttgcacaag tggagttcta tggtctggga ttcgaatatt
     1741 tttcggacta ccaaaaatgg atagaccggg tcacgaagga agacgtgcag cgcgtggcga
     1801 aacaatatct ggaccccaaa cgatacgcct tagtggtggt gggcaacatt gccaaagcca
     1861 aggtcaagca ctagcaggct gctgaaaaag tccgccagcg gcgttctcgc ttcgctcaga
     1921 ggctcaacgt accgaagcgt acgcctcgcc tcttcgctcg ctgcggcctt gcctgcggaa
     1981 cggcgcgtct tggcgcgctg gggttgggcg ggtgaaaggc agcctttttg agcatcctgc
     2041 gcagtcttcc tacttatcct tgatctgatc ggtgagtgac cgatgcctca cactcgttgg
     2101 gaaggagtca aaccatggac caaggcgccg aactcgatgc actgattgcc agaatgattg
     2161 aagactatgt gtcgcgcaat cgggcggcca aggtgctgcg ggattcgctg gacaaggcgg
     2221 gagtcggttt ctacccggtt gcagaccatc tgacgctgcg gacgatgaat atcgaccggt
     2281 gcgcggagga atacacgaag ctcgggtatg ggtacagcga aacgatcgag tatgaagact
     2341 ggtatgccaa ggtctatcgg aaggtcgggt accccgcgct tttcgtggat caagcctatg
     2401 ccgatgatcg gggtcgcacg agtatcattc ccggttgggt caagacattc ggcgatcagg
     2461 tgtaccacca tgtggc
//
LOCUS       DATIPF010000422         4847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1143174, whole genome shotgun sequence.
ACCESSION   DATIPF010000422 DATIPF010000000
VERSION     DATIPF010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143174"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..940)
                     /locus_tag="VLL06_10545"
     CDS             complement(62..940)
                     /locus_tag="VLL06_10545"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HSS31446.1"
                     /translation="MNEWGPGLATILGIVEGLTEFLPVSSTGHLILVGHVLGFEGDLA
                     ASAEISIQLGAILAVIAYERHKLASLIGQAAREQRAVSAMIRTRGTIPWSTVLRTSWA
                     THWNLWFLMGLGLAFLPAAAIGFLTHSWIKSHLFTPQTVAASSIVGGLIILAVEARRD
                     QARIHTLEQVGLASAFWIGLAQCASLLPGMSRSGSTIIGGLLVGLDRKVATEYSFFLA
                     LPTLIVATCYQMWKSRDVFQQDDYLALGIGMVVSFVVAWIVIAAFLSFVKRHTLRPFA
                     YYRILMGIAVFYIFGF"
     gene            1223..1927
                     /locus_tag="VLL06_10550"
     CDS             1223..1927
                     /locus_tag="VLL06_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31447.1"
                     /translation="MKSGWMAMVVMIGAASLVGCVSQKKYEEAMVDVDSAKFELERTR
                     AQKNALEQQVKTLKDLNVKFGNEAQVSHDELERIQHGRDKERGSVEGRTKELEDRVKQ
                     LTFQNRAVRHEYEDVKRHNDTLKALVARYQKELKDQSRPVTGSPAPSVPPVSPAPGAE
                     PAPPAATASQPMSSSMNVNKASAGDMVLVLGLPQDVADRIVAGRPYRVKGELVAKNVV
                     PKDTFDRIKERISVSP"
     gene            2086..3363
                     /locus_tag="VLL06_10555"
     CDS             2086..3363
                     /locus_tag="VLL06_10555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSS31448.1"
                     /translation="MRRIVPIIGVLAVALTIGGYVFFNGERKAPVRYRNAAVERGPVI
                     SLVTATGTINPVVSVQVGTQVSGMIKSLHADFNSVVKAGDIVAVIDPEPFRARRDQAA
                     SNLEMSRANVARAKTDLAQRKRELDRVKSLVAQQYVSQNDVDVATTTFQAAESQVNVT
                     GAQVKQAEAALNASELDLKYTVIRSPVNGIVVARNVEVGQTIAASFATPNLFLIALDL
                     TKMQVDTNVSESDIGGITEGKEATFTVDAYPGHQFAGMIRQVRLAPINVQNVVTYNVV
                     VSVDNEDLRLKPGMTANVSIVVAQRDAVLKVPNAALRFTPPNAGQVDRSTPGSKPAKD
                     KGGEQTVGVGRGGTAPSRTIWKEGPAGELEPMRVHTGISDGVATEILSEGLSEGTLVV
                     VGIDRPRGDRSGSDLPPGFGSGGQRGSSRNRGM"
     gene            3487..4206
                     /locus_tag="VLL06_10560"
     CDS             3487..4206
                     /locus_tag="VLL06_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSS31449.1"
                     /translation="MGSLIECEDVWKIYRVGDVDVQALRGLSLTIEQGEFVAIMGSSG
                     SGKSTLMNIVGCLDQPTKGRYWLNGAEVDNLKPDQLAEIRNQQIGFVFQSFNLIPRTS
                     ALENAQLPLFYRGLSLKEQRLQAAAALGRVGLQGREHHYPTQLSGGQQQRVAIARALV
                     TSPSLLLADEPTGNLDTQSSREIMGILEALNRDEGITIVLVTHEMDLAAYAAREIMIQ
                     DGQIVSDRQTKARPPSTPVEV"
     gene            4399..>4847
                     /locus_tag="VLL06_10565"
     CDS             4399..>4847
                     /locus_tag="VLL06_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSS31450.1"
                     /translation="MSAFVWLTVLTAFRILGRNRLRAGLTMLGIVIGVAAVIAMVSIG
                     EGAKRAVQAQIATMGTNVIIIFPGVTSASGVRGSQGSAVTLTVSDALDLKKRIPLLAD
                     TGWARRDVMQSVYGNRNWNGPINGISPSYLTIRDWSFTSGGPFTQTD"
BASE COUNT         1102 a         1253 c         1513 g          979 t
ORIGIN      
        1 gggtccccgt tgagaatgct tttgggacga gaacgccgct ggcggacttt tcagcattct
       61 gtcaaaaacc aaagatgtag aataccgcga tccccatcag gatgcggtag taggcaaacg
      121 gtcgaagcgt atgtcgtttc acaaacgaga gaaaggcagc gattacaatc caggcgacga
      181 cgaacgacac gaccatcccg atcccaaggg ccagatagtc gtcttgctgg aagacgtccc
      241 gtgatttcca catctggtag caggtcgcca cgatcagggt cgggagggcg agaaaaaatg
      301 agtattccgt tgcgactttt cggtcaagcc ctacgaggag ccctccgatg atcgtggagc
      361 cggaccggga catgccgggt agcagcgagg cgcattgggc caagccaatc cagaatgccg
      421 atgccaggcc cacctgttcg agtgtgtgga tccgtgcctg gtcacggcgt gcttcgacgg
      481 cgagaataat caagcctccg acaattgacg aggcagccac ggtttgaggc gtaaagagat
      541 ggctcttgat ccagctgtgc gtgaggaacc cgattgcggc tgccggtaag aatgcgagtc
      601 cgagtcccat gagaaaccag agattccaat gcgtcgccca ggaagttctc aggacggtcg
      661 accaggggat tgttccacgt gtccgaatca tggcggaaac ggcccgctgc tcccgagccg
      721 cttgtccgat caaggatgcg agcttatgcc gttcataggc gatgacggcc aggatggcgc
      781 caagctggat cgaaatctcg gcgcttgctg cgaggtctcc ttcgaacccg aggacatggc
      841 caacgagaat caagtggccg gtcgacgaga cgggtaaaaa ctctgtcagt ccctccacga
      901 tacctaagat cgtggcgaga ccaggacccc attcgttcat gcgatgtctt tctgggacag
      961 gttgcgtccg gatggcaagt ctcgggcatc ttcacaaagc ggcggcagtc cgtcaagcag
     1021 acggagaatt tgtcgcagac ttccgatgat cgatgagggg cagaggacct gtaaatctat
     1081 tgacaaaagg acaggcatgg catacatcca agagtatacg tatgggtgtc atactacccg
     1141 ctcatgccga ccggcatgtt gagtggagag tgagtgtcga catctgtatc aaataccacg
     1201 ttctgtggaa ggggaggttc gcatgaagtc agggtggatg gcgatggtcg tgatgatcgg
     1261 cgcggcgtca ctggtcggct gtgtcagtca aaagaaatat gaagaggcga tggtcgacgt
     1321 cgattcggca aagtttgagc tggagcggac acgtgcacag aagaatgcct tggaacagca
     1381 agtcaagacg ctcaaggatc tcaatgtgaa attcgggaac gaggctcagg tctcccatga
     1441 cgagcttgag cgtattcaac atggccggga caaggaacgc ggcagtgtcg agggccggac
     1501 caaggaactc gaagaccgtg tgaagcagct cacgttccag aatcgtgcgg tgcgtcatga
     1561 gtacgaggat gtgaagcggc ataacgacac gctcaaagcc ttggtggctc gctatcagaa
     1621 agagttaaaa gatcagtcac gtccggtgac agggtccccg gcaccctctg tgccgcccgt
     1681 gagtcctgct cccggagcgg agcctgctcc tccggcggcg acggcttctc agcccatgtc
     1741 ttcctccatg aatgtcaaca aggcgtccgc cggtgacatg gtgctggtgc ttggcctccc
     1801 tcaagatgtc gcggaccgca ttgtcgcggg tcgtccctac cgagtgaagg gtgagttggt
     1861 cgccaagaac gtggtaccca aagatacctt tgatcggatc aaggaacgca ttagcgtcag
     1921 tccctaatca gcgccatcgt tctgcacgtg tgacaggccg ttcgccgttc ggcggatggc
     1981 ctgtttgttt ttgcggaact gtgtatagaa gctgagtcag gtgattccct tcagccttgt
     2041 tgatagaatg tgttccacgt cgggaaggaa ctggagaacg agtcgatgcg ccgtatcgtc
     2101 ccgatcatag gggtccttgc cgttgcgttg acgatcgggg gctatgtctt cttcaatgga
     2161 gagcggaagg ctccggtgag atatcggaac gccgcggtcg aacgaggccc cgtcatttcg
     2221 ctcgtgactg caacgggaac gatcaatcct gtcgtctcag ttcaggttgg gacgcaggtg
     2281 tccggcatga tcaagagcct gcatgccgat ttcaattcgg tggtgaaggc cggtgatatc
     2341 gtggcggtga tcgatcccga accgttcaga gcccgtcgag atcaagcagc cagcaatctt
     2401 gaaatgtcta gagcgaatgt cgcgagagcc aaaaccgatc tggctcaacg taagcgcgaa
     2461 ttggatcggg tgaaatcgct ggtggcgcaa cagtacgtct cccagaacga cgtcgatgtc
     2521 gccacgacga cgttccaagc cgcagagtca caagtgaatg tgacaggggc gcaagtgaaa
     2581 caggctgagg cggcgctgaa tgcctcagag ctggacctga agtacacagt cattcgctct
     2641 ccggtaaatg ggattgtggt cgcaagaaac gtggaggtcg gtcaaacgat cgcggccagt
     2701 tttgccacac cgaacctctt cctgattgcg ctcgatctga cgaagatgca agtcgatacc
     2761 aacgtcagtg agtcagatat tgggggaatc accgaaggga aagaggcgac cttcacggtc
     2821 gatgcgtatc cagggcatca atttgccggc atgatccgtc aggtccgtct tgcaccgatc
     2881 aacgtgcaaa atgtggtgac ctataacgtg gtggtcagcg tcgataatga ggatctccgc
     2941 ctgaagccgg gaatgacagc gaacgtctcc atcgtggtgg cgcaacggga cgctgtactc
     3001 aaagttccga atgccgcgtt gcgattcact cccccgaatg cgggccaagt cgatcgttcc
     3061 acgcccggca gcaagccggc caaagataag ggaggagagc agacagtagg ggttgggaga
     3121 gggggcacgg cgccaagccg cacaatttgg aaagagggac cggccggaga actcgagccc
     3181 atgcgcgtcc acacgggtat ttctgatgga gtggcaacgg agatcctatc ggagggcttg
     3241 tctgaaggca cgctggtggt cgtggggatc gaccgaccaa gaggcgatcg gagtgggagc
     3301 gatctgcctc caggatttgg aagcggagga cagaggggct cctcgcgcaa tcgtgggatg
     3361 taggagatgt gggctgggtg tgggtgcttt ctgtgctcgc gggacgtgcg caggagaaaa
     3421 tacccaatcc ctgcccattt cttttgagat ggtagagacg gaaccagatc agatcgaagg
     3481 acgtgtatgg gctcgctgat cgaatgtgaa gatgtgtgga aaatctatcg agtcggcgac
     3541 gtggatgtcc aagcgttgcg tggcctgagc ctcacgattg aacagggtga gttcgtcgcc
     3601 attatggggt cttccgggtc cggcaagtcc actctgatga atattgtggg ctgcctcgac
     3661 caaccgacga aggggcgtta ttggttgaac ggggcagaag tggacaatct gaagcccgat
     3721 cagttggccg agatacgcaa tcagcaaatc gggttcgtct ttcagagctt caatctcatt
     3781 cccagaacca gtgccctcga aaatgcgcag cttccattgt tctatcgggg gctctcgctc
     3841 aaagaacagc ggctacaggc tgcagcagcg ttagggcggg tggggctgca gggcagagag
     3901 caccattatc caacacagtt gtccggcggg cagcaacagc gcgtggcgat cgcgcgggca
     3961 ctggttacat cgccttcgtt actccttgcc gatgagccga ccgggaatct ggacacccag
     4021 tcgagccggg agataatggg cattttggaa gccctcaacc gggatgaagg gatcacaatt
     4081 gtccttgtga cgcatgagat ggaccttgcc gcctacgccg cgcgggagat catgatccag
     4141 gacggacaga tcgttagcga ccgtcagacg aaagcacggc caccatcaac tccggtcgag
     4201 gtatagcctg cattaatcat gaacgcttct actacaatcg ccgacccgct gctacgacga
     4261 gccttcggcc agtgggctcg cccatcggat cctcaacgta ctacacgagt ccgcctcggg
     4321 tcctcacggt tccgcgcgct ggtctcgcga cgcggctccg cgatttcgcg acgaaccgtc
     4381 atgaataatg agggctaaat gtcggcattt gtctggctca cggtcttgac agccttccgg
     4441 attcttggac ggaatcggct gcgcgccggt ctcacgatgc tgggaattgt catcggtgtg
     4501 gcggcggtca ttgcgatggt gagtatcgga gagggcgcca agcgggccgt acaagcgcag
     4561 attgcgacga tgggcacgaa cgtcattatt atctttcccg gagtgacgtc ggcgagcggt
     4621 gtccgaggaa gtcaagggag tgccgtgacg ctgaccgtct ccgacgcctt ggacctgaaa
     4681 aaacgcattc ccttgctggc cgataccggt tgggccagac gcgatgtcat gcaaagtgtc
     4741 tatggaaatc ggaactggaa tggaccgatt aacgggatct ccccgagcta tctcacgatt
     4801 cgagactggt cctttacgag tggaggtccc ttcacccaga ctgattt
//
LOCUS       DATIPF010000423         1858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_612777, whole genome shotgun sequence.
ACCESSION   DATIPF010000423 DATIPF010000000
VERSION     DATIPF010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612777"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VLL06_10570"
     CDS             <1..337
                     /locus_tag="VLL06_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSS31451.1"
                     /translation="HQLEVSTVMPGMQGKGIRLKALLDVPAMAIKADHVTIHSGDGNY
                     SACLTLEQAKAFGVLVYELDGAPFPESKGGPFRLITPGLGDLCANVKGVARIEVTVGT
                     GMDTRPTNC"
     gene            complement(543..782)
                     /locus_tag="VLL06_10575"
     CDS             complement(543..782)
                     /locus_tag="VLL06_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31452.1"
                     /translation="MMKTKAIFYHAGCPVCVAAEHSVANALDPAKYTVESVHLGTSKS
                     RVKEAEAAGVKSVPALVMNGAAFHINFGAGIDALK"
     gene            complement(1060..>1858)
                     /locus_tag="VLL06_10580"
     CDS             complement(1060..>1858)
                     /locus_tag="VLL06_10580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HSS31453.1"
                     /translation="YPLIYGHWGLPALGITGAAVATGLAEGIGVAYLLWACRKIFKIS
                     LHVRRDLLRSTWQIGASVSGERMFQQAGILLYTKIVLVYGTVTYAAHQVGLSIESLSF
                     LPGYGFAIAAATMVGQSIGAGKYVRAKLENWEANRLASITMAAMGVLFFFFPYMLLRA
                     FTNDEAVVELGTVFLRIVAILQIPLALTMVLAGSLRGAGDTRFIMFTTTIGMWGVRLP
                     LAIIAGPWLALDVLFVWTAMIADWTVRMGLLLWRYRSERWKAIQVMP"
BASE COUNT          420 a          505 c          533 g          400 t
ORIGIN      
        1 gcatcagctt gaggtttcca cggtgatgcc aggtatgcag ggtaaaggca ttcgcctcaa
       61 ggccttgctg gatgtaccgg ccatggcgat caaggccgac catgtgacga tacattcggg
      121 ggacggcaac tattcagcct gtctcacact ggagcaagcc aaggcatttg gggtgttggt
      181 gtatgagctc gatggcgcgc catttccgga atcgaaaggc gggccgtttc gactaatcac
      241 ccccgggctg ggcgatctgt gcgccaatgt gaagggtgtt gcgagaatcg aggtgaccgt
      301 cggaacgggg atggacacgc gcccaacgaa ctgctagcag gatgctgaaa aatcccgcca
      361 gcttcgttct cgggtcatcg aaatcctcaa cgtaccccag agggtacgct tccggtttcg
      421 actcgcctgc ggccttgctg gacgggcttt ttgagcatcc tgcgaatctg tggggcgcaa
      481 aaagaggggg cgtgtcgtga gacacgcccc ctgtgttttg agccttcagt tgtcagccag
      541 agttatttca aggcgtcaat gccggcgccg aagttgatgt gaaatgccgc gccattcatc
      601 acgagcgctg ggacagactt gaccccggcg gcttccgctt ctttcacccg cgacttgctc
      661 gtgccgagat ggacgctttc cacggtgtat tttgccggat cgagcgcgtt ggcgacgcta
      721 tgctcggccg cgacacagac tggacagccc gcgtggtaga aaattgcttt cgttttcatc
      781 ataatctccc ttcaaatgtg gtggttctga atacggccgt tgtcgtctag acagaataat
      841 aaccaggctc gtctctggct agcaggctct gttctgtgcc ccgataatta ttgatggagt
      901 aacttaccat aaggaagatg ataaaaaaac cccctgtccg tggacagggc ttctcgaaga
      961 tgtgagcggg acaaaactgg ttcatctcat tcaaccacat acaccaaata gactagagaa
     1021 acaaatctgt tcttgcatgt cgagtgagtc tcgtccgttt cagggcatga cctgaatggc
     1081 tttccaccgt tccgaacggt agcgccaaag cagaagtccc attcgaactg tccagtctgc
     1141 aatcatcgcg gtccatacga agagcacatc gagcgcaagc cacggacctg caatgatcgc
     1201 gaggggcagg cggactcccc acatgccgat cgttgtcgtg aacatgatga accgggtatc
     1261 tccggctcca cgcaatgatc cggccaggac catggtgagg gcgagcggaa tttgtaagat
     1321 cgcgacgatg cgcaagaata ccgtcccgag ttcgacaacc gcctcgtcat tggtaaaggc
     1381 gcgcaggagc atatagggga agaaaaaaaa gagcaccccc atcgcggcca tggtgatgga
     1441 agctagtcga ttggcctccc agttttccag tttggcccgg acatattttc ccgcgccgat
     1501 actttgcccc accatcgtgg ctgccgcgat cgcgaagcca tatcccggca ggaacgacaa
     1561 ggattcgatc gacaggccga cttgatgtgc ggcgtaagtg actgtgccat atacgaggac
     1621 gatctttgta tagagcagaa tgcctgcttg ctgaaacatc cgttccccgg agaccgacgc
     1681 tccgatttgc caggtggagc gcaacagatc tcgtcggacg tgcagtgata tcttaaagat
     1741 ctttcggcag gcccagagaa ggtaggccac gccgattcct tctgccagac ccgttgccac
     1801 ggctgctccg gtgatgccca aggcagggag gccccagtgt ccatagatca ggggatag
//
LOCUS       DATIPF010000424         2945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_80129, whole genome shotgun sequence.
ACCESSION   DATIPF010000424 DATIPF010000000
VERSION     DATIPF010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80129"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VLL06_10585"
     CDS             <1..882
                     /locus_tag="VLL06_10585"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HSS31454.1"
                     /translation="GPREELERHRIPLHIELPGVGQNLQDRYEVGIVYKMKENFSTLE
                     GATFQADEQKDQAYREWKYASSEERKQGKGFLYSSNGVVISILKRSSVAENEKHDPDL
                     FIFGLPSDFRGYEQGYTKNITVKDRFTWLILKGHTHNTGGGVKLRSNNPRDTPAINFN
                     YFEEDNDPTRGKKDLDAMVEAVGLIRKIMDRLVAEGQAEEIWPGPDVSEHTLREWIMN
                     EAWGHHASCSCKIGAEGDGLAVLDNNFRVRGTTNLRVVDASIFPYIPGFFIVTPIYMI
                     AEKASEIILADATQVST"
     gene            1045..1302
                     /locus_tag="VLL06_10590"
     CDS             1045..1302
                     /locus_tag="VLL06_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31455.1"
                     /translation="MAYLRYSRDCDWHVFEEAKQGEAKNRLAVWHKDHEAQGASYTVS
                     MIQKMLELEDYSHIPGYQPQDKRILRKAFAAWLSEQSSEEI"
     gene            1577..2173
                     /locus_tag="VLL06_10595"
     CDS             1577..2173
                     /locus_tag="VLL06_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31456.1"
                     /translation="MVAMLWVGSTLVLAQGRLTDVLVSVVDDKVVAVTGVGQSEIDLS
                     VGETVVSTKAQGLTALAITSTRLLGFSSQLRHWGEQTLEAEEHVTRSQVLREFCVVAT
                     DRHLYGFQETLAHWTGEALGGSERVQEVRANGHLALAVTTERLVGFSALMSGFHTMPL
                     QGDERVRGIEQTGDAFLVKTTRRTLMFRSRMSGWTEMS"
     gene            complement(2303..>2945)
                     /locus_tag="VLL06_10600"
     CDS             complement(2303..>2945)
                     /locus_tag="VLL06_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSS31457.1"
                     /translation="LEASIPGVSSAIFFNQGQCCCAGSRLFIEKGIFDKVVAGVAEGA
                     KKIKVGPGMDLATQMGPLVSDEQQRRVLGYLESGFSEGAKAVVGGHKLGNKGYFIEPT
                     VLVNTTQKMKITQEEIFGPVVCAMPFTDLDEVLPAANESIYGLAAAVWTRDVSKAHRI
                     ASELRAGSVWINCYNIFDAALPFGGYKQSGWGREMGHDALELYTEVKAVTVRL"
BASE COUNT          632 a          780 c          863 g          670 t
ORIGIN      
        1 ggcccgcggg aagaactcga acgacaccgc attccactgc atatcgaact gccgggggtc
       61 ggccagaact tgcaggaccg ttacgaagtg ggaatcgtct acaagatgaa ggagaacttc
      121 tccactttgg aaggagccac gtttcaagcc gatgagcaga aggatcaggc ttatcgggag
      181 tggaagtatg cgagctctga agagcggaaa caaggcaagg gttttctgta ctccagtaac
      241 ggtgtggtga tctcgattct gaaacgttca tctgtcgccg aaaatgagaa gcacgatccc
      301 gatcttttta tcttcgggtt gccttcggat ttcagagggt atgagcaggg ctatacgaag
      361 aatattaccg tgaaggaccg cttcacttgg ctgatcttga agggacatac ccataataca
      421 ggtggggggg tgaagctgcg ctccaacaat cctcgcgata cgccggccat taatttcaat
      481 tatttcgaag aagacaacga tccaaccagg ggcaaaaaag acttggatgc catggttgaa
      541 gcagtcgggt tgattcggaa aatcatggac cgtcttgttg cggaagggca agccgaggag
      601 atctggccag gaccggacgt ctcagagcac acattgcggg aatggattat gaacgaagcc
      661 tggggccacc atgcatcgtg ctcttgtaag ataggagctg aaggcgatgg tttggccgtc
      721 ttagacaaca attttcgggt ccgtggcacc acgaacctgc gcgtggtcga tgcatcgatt
      781 ttcccctata ttcctggatt tttcatcgtt accccaatct atatgatcgc ggaaaaggcc
      841 agcgagatca ttctcgctga tgccacacag gtatccacct gagtgatagc acctgtgtaa
      901 tgatccagct ctaggttctg gccaggctct cgttcatctt gacctgtgct gagactcagc
      961 gtagggtttt gggtgaacga tcacacacat cgttccacaa acggttccca gcgcgcgaca
     1021 ggttcggcac cgtgagttgt ctgtatggcc tatcttcgat attcacgaga ctgtgactgg
     1081 catgtcttcg aggaagccaa acagggcgag gccaagaatc gtctggccgt gtggcacaag
     1141 gaccatgagg cacagggtgc gtcgtatacc gtgagcatga ttcagaaaat gctggagttg
     1201 gaagattatt cccacatccc cgggtaccaa ccgcaggata aacgcattct ccgcaaagcc
     1261 ttcgcagcct ggctgagcga acaatcaagt gaggaaattt agtgctggct tgttcctaag
     1321 tgacgccccc attgtcgtgg ttggtatcga atgtgcagac aaggggtctg gtgttgtcat
     1381 ccaatatatg tgggaaccta tcggcgcgtg ggggcacttg tgatccgatg ttcgagtcat
     1441 cttattaaca ggtcctgcct ctggcggtcg ctgttctgaa ctggtgatgg gatcgatgat
     1501 ggttcatctg atgcgaccat gttccccgtc cgggtgtctg gctccagcct tgcgcgccgg
     1561 aatcctatgc tttggtatgg tcgcgatgct gtgggtcggc agcacgctgg tgctcgccca
     1621 aggcaggctc accgatgtgc tggtgtcggt ggtcgacgat aaagtggtcg cagtgaccgg
     1681 agttggccaa tccgagatcg acctctcagt cggtgaaacg gtggtaagta ccaaggccca
     1741 gggtctgacg gcgttggcga ttacctccac gagattgttg ggattttcct ctcagcttcg
     1801 gcattggggc gaacagacgc tggaggcgga ggagcatgtc accaggtcgc aggtgcttcg
     1861 agaattctgc gtcgtggcca cggatcggca tctctatggt tttcaagaaa ccctcgcgca
     1921 ttggaccggc gaagctttag gcgggtcaga acgggttcag gaagtacgtg ccaatggcca
     1981 cttggctctc gctgtgacga cggaacggtt ggtggggttc tcggccttga tgagcgggtt
     2041 tcatacgatg cctcttcaag gagacgagcg tgtgcggggc attgagcaga ccggcgatgc
     2101 gtttcttgtc aagacgaccc gtcggacgct gatgtttcgc agtcgcatgt cgggctggac
     2161 tgaaatgagc tgagggcgaa cggcatgcgc cacgaagata tgttgccggg ctccgagcga
     2221 agggccgcac gtactgagcg cacccacatg cttacagccg ctggctccct tcccgcctat
     2281 tctcccgttc tccagcgatg ggtcacagcc gcacggtgac cgctttcacc tcggtataca
     2341 gctccagtgc gtcgtgaccc atttcgcggc cccatccgga ttgtttgtag ccgccgaagg
     2401 gcagcgcggc atcgaagata ttgtaacagt tgatccatac cgaccccgcg cgcagctcag
     2461 acgcgatgcg atgggccttg ctgacatcgc gggtccatac ggccgccgcc aggccgtaga
     2521 tggactcgtt cgctgccggg agtacttcgt ctaagtccgt aaacggcatg gcgcagacaa
     2581 ccggcccaaa gatttcctct tgcgtgatct tcatcttttg cgtggtgttg accagcacgg
     2641 tcggttcgat aaagtagccc ttattgccca gtttgtgtcc gccgacgact gccttggcgc
     2701 cttcggagaa gcccgactcc aagtagccga gtacccgccg ttgctgctcg tccgacacca
     2761 gcgggcccat ttgggtggca agatccatac caggccccac cttgatcttc ttcgcacctt
     2821 cagcaacacc cgctaccacc ttgtcgaaga tacccttctc aatgaagagc cgggagcctg
     2881 cgcagcagca ctgaccctgg ttgaaaaata tcgcgcttga gactcccggt atgctcgcct
     2941 ccaga
//
LOCUS       DATIPF010000425         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_875665, whole genome shotgun sequence.
ACCESSION   DATIPF010000425 DATIPF010000000
VERSION     DATIPF010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875665"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..997)
                     /locus_tag="VLL06_10605"
     CDS             complement(155..997)
                     /locus_tag="VLL06_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSS31458.1"
                     /translation="MIRRTRSWPDHARAFIGSCLSEPLYRLFRLVPHLEVGLSTHDIT
                     RLTYVHGLLAGRRAVHLSDLHLDCYQTRHDRIVAAIGELRPDWIFVTGDLLNVPEGLP
                     HVFRFLAALREFAPVFVTLGNHDHYSGVPIDQYGELADRHKITLLVNQATFIPTGCGE
                     IAIAGVDDPSLHRADLSCLPPRAQDRFILLLAHAPNILDQLEDHHAVDLILCGHSHGG
                     QWRIPGVPTFWLPPGCNGRIEGHHGTAGRRLYVNRGLGWSFLPLRLNCTPEIACIEWV
                     AEET"
     gene            complement(994..1635)
                     /locus_tag="VLL06_10610"
     CDS             complement(994..1635)
                     /locus_tag="VLL06_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31459.1"
                     /translation="MRLRSATAQWPKILPLAVGVLLTVLLYALFPGDSSSRENEIFQQ
                     FTQHIGRQIHKDKQAFAQANHCVSWFYKAKKTPPASVQGINWNATRLSQPALDCSRDY
                     PGGMDDARDDLAKTQASLSVSLTFYEFALVADQNDDATYSPAEIQDLFRSLSLSYHNG
                     DSILMQVSALTERFDNWYQKRNMDALMQGMSDLYERGYRVTPFDRVELDRVMR"
     gene            1731..>2063
                     /gene="truD"
                     /locus_tag="VLL06_10615"
     CDS             1731..>2063
                     /gene="truD"
                     /locus_tag="VLL06_10615"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HSS31460.1"
                     /translation="MTMPFLTDTLPGIGGQMRTIPEDFQVEERPLYLPCGEGEHLYVK
                     IRKRLLSTPDLIQRLSSILGMKAQAIGVAGLKDARAVTTQMVSLLGVTPDRLDRLKVD
                     EQLLSVEVL"
BASE COUNT          475 a          524 c          639 g          425 t
ORIGIN      
        1 gtacgttcct gcgccgcgct gggtttgggg cggttacgtg ccagtgaggc aaaggagaag
       61 ctgacgactg tgatggagca ggattgggat caaaccgtca ggagccggtc gaaggaagcg
      121 ttggagcgga tcaagcagtc atgaatggtg caggctaggt ctcttcggcg acccattcga
      181 tgcaggcgat ttccggcgtg caattcaatc gcaagggaag aaacgaccat ccgagcccgc
      241 gattgacata cagccgtcgg ccggccgttc cgtgatgccc ttcgatccgc ccattgcatc
      301 cgggagggag ccagaacgtc ggcacgccgg gtatgcgcca ttggcctccg tggctatgcc
      361 cacagaggat cagatcgacg gcgtggtggt cctccagctg atcaaggatg tttggcgcat
      421 gggccagtaa gagaatgaat cgatcctgtg cgcgtggtgg cagacagctc aagtcagcgc
      481 gatgtagcga gggatcgtcg acgccggcaa tggcgatttc cccgcacccc gtaggaatga
      541 aggtggcctg attgaccaaa agggtgatct tgtggcggtc ggcgagctcg ccgtactgat
      601 cgatcggcac gccactgtag tggtcatggt tgcccagggt gacaaagact ggggcgaatt
      661 ctcgcaaggc ggcgagaaat cgaaatacat gcggcagccc ctccggtaca ttgagtagat
      721 cgccggtcac aaaaatccag tcgggtcgaa gttcaccgat ggccgcgacg atacgatcat
      781 gtcgcgtctg atagcagtcc agatgcagat cgctgagatg cacggctcgc cggcctgcca
      841 agaggccgtg cacataggtg agccgtgtaa tatcgtgtgt cgagagtccg acttccaagt
      901 gagggacaag acgaaaaagc cgatagaggg gttcactcag acaagatcca ataaaggcac
      961 gggcatgatc gggccaggac cttgtgcgtc gaatcatctc atcacccggt ccagttcaac
     1021 gcgatcgaag ggggtcacgc gatagcctcg ttcatagagg tcgctcatgc cttgcatcaa
     1081 ggcgtccata ttgcgtttct ggtaccaatt gtcgaaccgt tccgtgagtg ccgaaacctg
     1141 catcaggatt gaatccccgt tatggtacga gagtgaaagg gagcggaaca agtcctgaat
     1201 ttctgcagga ctataggtcg catcatcatt ctggtcggcg accaaggcga attcgtagaa
     1261 ggtgagactg acggagagcg atgcctgtgt tttggcaaga tcatcccgag catcgtccat
     1321 cccaccagga tagtcgcgag aacagtccaa ggcaggttgg gataaacgag tggcgttcca
     1381 gttaatcccc tggacggagg cgggaggcgt ctttttcgcc ttataaaacc atgagacaca
     1441 gtggttcgcc tgcgcaaagg cctgtttgtc tttgtggatc tgccggccga tgtgttgggt
     1501 gaactgttga aagatttcat tttctctact tgaggagtcc cctgggaaca gcgcatagag
     1561 cagaacggtg aggagcactc ctaccgctaa cgggaggatc ttcggccatt gggctgtcgc
     1621 agagcgaagt cgcataggaa aagtatacca tgcgagttgt gtcatccatc tcaaaattga
     1681 ctcggtgatg aaggcgatca cacgcgaatg acacaggggc cgcttgcacc atgaccatgc
     1741 cgtttttgac agacacattg ccgggcatcg ggggacagat gcggacaatc ccggaagatt
     1801 ttcaggtcga ggaacgaccg ctctatcttc cttgcggaga gggcgagcat ctctacgtca
     1861 aaatcagaaa acgcctgctc tcgaccccgg accttattca gcgattgtct tcgatcttgg
     1921 ggatgaaagc gcaagcgatt ggggtggcgg gcctcaaaga tgcccgggcc gtcaccacgc
     1981 agatggtctc actgctcggt gtcacgcctg accggttgga tcgactgaag gtagacgaac
     2041 agctcttgtc cgtggaagtg ttg
//
LOCUS       DATIPF010000426         5557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1494522, whole genome shotgun sequence.
ACCESSION   DATIPF010000426 DATIPF010000000
VERSION     DATIPF010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494522"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1228)
                     /gene="pqqE"
                     /locus_tag="VLL06_10620"
     CDS             complement(131..1228)
                     /gene="pqqE"
                     /locus_tag="VLL06_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HSS31461.1"
                     /translation="MTNAYRPYTLIAELTYRCPLRCPYCSNPLGYAGQGDEIDTDTWL
                     RVFQEAEALGVVQLNLTGGEPLLRDDLERLIEEASRLELYTNLITSGIPLTFERLSRF
                     AALGLNGVQVSVQSSRPTESDRIAGAPSFHQKLEVMQWVASLKLPLTLNVVLHRSNIG
                     EIEELVALAERVSADRLELANAQYLGWALKNRGELPPTREQVETARTLAADSRERLRG
                     TMEVLFVTPDYYSGYPKSCMNGWGRRFIVVNPEGLVLPCHLAHTLPGLRFDNVMKRDL
                     ADIWHHSDAFNQFRGDAWLPDACRNCDRHTKDFGGCRCQAYHVTGHAGATDPACSLSP
                     DHGYIESARNQSNHLAGRPILFEYRTGRGFS"
     gene            complement(1225..1506)
                     /gene="pqqD"
                     /locus_tag="VLL06_10625"
     CDS             complement(1225..1506)
                     /gene="pqqD"
                     /locus_tag="VLL06_10625"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HSS31462.1"
                     /translation="MNGDIVRPQFNRKVRLRFDRRTGHYLLLYPETGLELSRTATEIA
                     RLCTGEWTVSEIAQHLATIHTEISPVEIMRDVCGFLKTLAARGLLQGFK"
     gene            complement(1475..2251)
                     /gene="pqqC"
                     /locus_tag="VLL06_10630"
     CDS             complement(1475..2251)
                     /gene="pqqC"
                     /locus_tag="VLL06_10630"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR02111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HSS31463.1"
                     /translation="MKDGQHRDPLSHDGFVEWLTREGSTRYHDHHPFHTLMHEGNLTK
                     VQLQQWVLNRYYYQTRIPIKDALIMSKSEDPSFRRTWLRRIQDHDGQQEGEGGLMSWL
                     ELARGVGLDVEEVRSLRSVLPGVRLACDGYVQFVRDSSLLEAVASSLTELFAPTLMAR
                     RLEAWKHHYPWVSSDALGYFQMRRIRARLDSHQAVDFVVQHALTYEMQERCVRALITK
                     AEILWHLLDCLSMAYVEPGSGVKTPEYDQQHERRHRQTSV"
     gene            complement(2248..3171)
                     /gene="pqqB"
                     /locus_tag="VLL06_10635"
     CDS             complement(2248..3171)
                     /gene="pqqB"
                     /locus_tag="VLL06_10635"
                     /inference="COORDINATES: protein motif:HMM:NF003523.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HSS31464.1"
                     /translation="MIVRVLGSAAGGGFPQWNCACSHCQGLREGLINASPRTQESVSL
                     SADNNDWFLINASPDIHSQIESFAPLHPGTSRITPIQAIVLTNGDLDHCLGLLSLREN
                     HRLVIYSTESVRRGFTDGNVLYRTLQRFAGHVTWRTLKLGVEETLLCADGRPSGLTVE
                     AVAVPGKPPIHLEGLVSPSDPELNVGLRFRQCANGRVLAYLSAVGRITTSVFEGLADA
                     DCVMFDGTFWSSDELSAPGFLEKSAEDLAHWPVGGQRGSLALLSKVTAPQRVFIHINN
                     TNPMLREDSTERKIIEGAGWRVAWDGMEIRL"
     gene            complement(3190..3324)
                     /locus_tag="VLL06_10640"
     CDS             complement(3190..3324)
                     /locus_tag="VLL06_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31465.1"
                     /translation="MEWAVPSFVEVKMDAEISSYQDDFADSPDIKEPSAEGPVVTAPQ
                     "
     gene            complement(3515..4078)
                     /locus_tag="VLL06_10645"
     CDS             complement(3515..4078)
                     /locus_tag="VLL06_10645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HSS31466.1"
                     /translation="MTDMVKIGGIAGSLRKASYNRAILQAALKLVPQGSVLEILDLEG
                     IPGFNEDEEKAFPPQAREFKAKVKAADAILIVTPEYNYSVPGVLKNAIDWASRPYGDS
                     AWNAKPVAVMGASPGMLGTARAQYHLRQTFVYLNMFPLNQPEVMIANAAEKFDEQGNL
                     KDAATFKRIKQLLEALVDWTKILKHAT"
     gene            complement(4645..5505)
                     /locus_tag="VLL06_10650"
     CDS             complement(4645..5505)
                     /locus_tag="VLL06_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSS31467.1"
                     /translation="MMRIAVGVDWSDEAFAAVEQIGLLYRPDEVVLIHGVDLGVLTAP
                     MMAGTINMPGYDDLRQGLMDAGRQAIERCRTLLPTETPSIRTMCNVEYPAAFILDSAA
                     AVKADLIAVGTHDHSRVTEVFVGSISHSILLHATVPTLIVKGKARPVQRVLLAVENQE
                     DAGHLHTWLNAHPFKNSVKVTILSVVPPLPVIDPSLMVGLMDWPELGKRYAEQVVSDT
                     AHALAGPSFSVFPDVRIGDPVSTICEVGKSHDLIVMSSHGRKGLSRFLLGSISDGVVH
                     RAGCSVLVVR"
BASE COUNT         1310 a         1563 c         1438 g         1246 t
ORIGIN      
        1 ccgaacagtc gaccacttgc atacaacccc tccaacggca gagcggggtc ccttaccaga
       61 gcaaacacac cgagacagac gaccaggacg agcaatatca ccagactgat atccatacgc
      121 cggctcatgc ttaggagaac cctcgacctg tccgatattc gaatagtatt ggcctaccgg
      181 ccaaatgatt cgactggttc cgtgcggatt caatgtatcc atgatcaggt gagagagaac
      241 aagcaggatc ggtcgcgcca gcatgccccg tcacatgata ggcctgacag cgacatcctc
      301 caaagtcttt agtgtgacga tcacagttgc ggcatgcgtc gggtagccat gcgtctccac
      361 gaaattgatt gaaggcatct gaatgatgcc aaatatctgc gaggtcgcgc ttcatgacat
      421 tgtcaaaacg caatccgggc aatgtgtgtg ccagatgaca gggtagcacc agcccttccg
      481 gattcacgac aatgaaccgt cgtccccacc cattcataca ggatttgggg taccccgaat
      541 aatagtcggg cgtgacgaag agtacctcca ttgttccccg aagtcgttct cttgaatcag
      601 ccgccagggt tcgcgcggtt tcaacttgct cgcgcgtcgg cgggagctcc ccacggttct
      661 tgagagccca cccaaggtac tgcgcgtttg ccagctcgag ccggtcggcg gatacgcgtt
      721 cggccagagc gactagttcc tcgatctcac cgatgttgct gcgatggagc acaacgttca
      781 gggtcagcgg aagtttgagc gatgcaaccc actgcatgac ttcgagcttc tggtgaaagg
      841 atggtgctcc ggcaatccgg tctgattctg tcggccgaga actttgcaca gacacttgca
      901 caccattgag tccgagtgct gcaaaccgag acagccgctc gaaggtcaac gggataccgc
      961 tggtgatcag gttcgtgtac aactccagcc ggctcgcctc ctcaatgagc cgttccagat
     1021 catccctcaa caacggctcg ccccctgtca agttgagctg gacaacacca agtgcttcag
     1081 cctcctgaaa gacgcgaagc catgtgtcgg tgtcaatttc atctccctgt ccggcatagc
     1141 cgagagggtt tgaacaatag gggcatcgta gcggacaccg gtaggtgagc tcggcgatga
     1201 gcgtgtaggg acggtaggcg ttagtcattt gaagccttga agcagaccgc gggccgcaag
     1261 ggtcttgaga aagccacaca catcccgcat gatttcgaca ggagaaatct cagtatggat
     1321 ggttgccaga tgctgcgcaa tttcgctgac cgtccattct cccgtgcaaa gacgtgcaat
     1381 ctccgtcgcg gtcctgctca gctcaagacc cgtttcaggg tacaggagca ggtagtgacc
     1441 ggttcgccga tcgaaacgaa ggcgaacttt acgattaaac tgaggtctga cgatgtcgcc
     1501 gttcatgctg ttggtcatat tcaggtgtct ttactcctga tcctggttcg acataggcca
     1561 tagacaggca atcgagtagg tgccacagaa tttcggcctt cgtgatcagg gcccgcacac
     1621 atcgttcctg catctcatac gtgagggcgt gctgaacgac gaagtcaacg gcctgatgcg
     1681 aatcaagcct ggcacggatc ctacgcatct gaaaataccc caaggcgtct gaactcaccc
     1741 agggataatg gtgtttccac gcctcgaggc gtctggccat gagcgtcgga gcaaacaact
     1801 cggtgagtga tgaagccact gcttcaagaa gcgatgaatc acggacgaac tgcacgtatc
     1861 catcgcaggc gagtctcacg cccggaagca ctgaccgcaa gctcctgacc tcctcgacgt
     1921 cgagacccac acctcgcgcc agctcgagcc atgacatgag tccgccttcc ccttcctgtt
     1981 gcccgtcatg atcctgaata cggcggagcc aggtgcgtcg aaagctcgga tcttcggact
     2041 ttgacatgat caatgcatcc ttgatcggga tccgcgtttg gtagtagtac cggttcagta
     2101 cccattgttg cagctggacc ttcgtcaaat tgccttcgtg catgagtgtg tggaacggat
     2161 gatggtcatg gtaacgggtg gaaccttcac gcgtgagcca ttccacgaag ccatcgtgtg
     2221 aaagtggatc tcgatgttga ccgtccttca tagcctgatc tccatcccgt cccacgcgac
     2281 cctccatcca gctccctcaa tgatcttgcg ctcggtggaa tcctccctca acatcggatt
     2341 cgtgttgttg atgtggatga acactcgctg aggagccgtg acctttgaga gcaaggcaag
     2401 acttcctctt tgccccccga cgggccagtg ggccagatcc tcggctgact tctcgaggaa
     2461 gcctggggcg gagagttcat cgctcgacca gaaggtgccg tcgaacatga cgcaatccgc
     2521 atctgccaac ccttcgaaaa ccgaggtcgt gatcctgccg acagcggata gataggccaa
     2581 tactctccca ttggcacatt gccgaaacct gagtcccaca ttcagttccg gatcgctagg
     2641 cgagacgagt ccttccaaat ggatgggagg ctttcctggg acagccacgg cttccaccgt
     2701 caaacctgat gggcgaccgt ctgcacatag cagggtctct tccacaccca gtttcagcgt
     2761 tcgccaggtt acatggccgg caaaccgctg cagcgttcga tacaagacat tgccgtcggt
     2821 gaatccgcga cgcacagact ccgtggagta gatcacaagt cgatgatttt cgcggagcga
     2881 aaggagcccc aaacagtgat ccaggtcccc gttggtgaga acaatcgcct ggatgggtgt
     2941 gatacgtgat gtccctggat ggagtggagc gaagctctca atctgcgagt gaatgtccgg
     3001 agacgcgttg atgagaaacc agtcgttatt atccgcgctc agactgactg attcctgtgt
     3061 tcgggggctc gcattgatca gcccttcccg taggccctgg caatgagagc aggcgcagtt
     3121 ccattgcggg aatcctcctc ccgctgccga ccctaggaca cgaacgatca tcacatccac
     3181 ccaccgatct tattgagggg cggtcacgac cggcccttct gcagatggct ctttgatgtc
     3241 ggggctgtct gcaaagtcat cttgatacga gctgatctcg gcatccattt tcacttccac
     3301 gaatgacggt actgcccatt ccatgatgcg tcctcctttt gtatcagcgg accgcctaca
     3361 cttaatgtga cattcgtccg atgcagctgc tctccacgcg acagaccatt cacagccaag
     3421 gataagcaaa ccggaacggg aggaggctat tgagtttttg ccatctgcgc taggggctca
     3481 tcactttcat ctggagcgaa agttgcttga ctaatcatgt agcgtgtttc aatattttcg
     3541 tccaatccac aagcgcctca agtaactgtt taattcgttt gaaagtcgcg gcatccttca
     3601 ggttgccctg ctcatcaaat ttctcggctg cattggcaat catgacctcc ggctggttca
     3661 gcgggaacat attcaagtac acaaatgttt gacgcagatg atattgcgca cgagctgttc
     3721 cgagcattcc gggagaagct cccatcacgg caaccggttt cgcattccat gcactgtccc
     3781 catagggacg agaagcccag tcgatggcat ttttcaggac tcccggcaca gagtagttat
     3841 actcgggggt cacgatcaaa atggcgtccg ctgctttaac cttagcttta aattccctcg
     3901 cctgaggtgg aaatgctttt tcctcatctt cattgaatcc agggataccc tcgagatcga
     3961 gaatctccaa aacactgccc tgaggaacga gcttgagcgc tgcctgaaga atggctctgt
     4021 tataagatgc ctttcggaga cttccggcta taccgccaat tttaaccatg tcagtcatgg
     4081 tgacaccttg ttttatggcg cccccttgtc caaccaaata attcctaaac ttacatttcc
     4141 gcaaaggtat agaagctagg aaagttccta gttatttgca aaaggaatct agccaaaagg
     4201 ggagtatggc ccgcccagac tcaagagaag caaccatgat tgcaacagag agacaaagga
     4261 ccaagcagcc tgccttacaa gtgagatggg aacagcgtgc ctatccgatg cgaccatctg
     4321 atcttggaat tagaatgaaa tggcgagggt cacacgaagc ggatgtatgt gtgcattctt
     4381 tgcggtgagg aattgatgcc taccctgctg catgctaagg atatatggct aaagtcctgg
     4441 tacgtttttg atccaggtgt gtacgccttc gccgcttctg tatcatcatc tttgaaagat
     4501 gcgaaatcag tccacaacaa cagctcagta aagcggcgta agatcgagac agctcctcac
     4561 gatatccctt caactcttca acactaattt cctaacccta gcgaacaagt tacaatctct
     4621 caacttcaaa ggatccacag aagttcagcg caccacgagc acggagcaac cggctcgatg
     4681 cacgaccccg tccgatatgc tgcctagtaa aaaacgactt agccccttcc gcccatgaga
     4741 gctcatcaca ataaggtcgt ggctttttcc tacctcgcat atggtactga caggatcacc
     4801 aattcgaaca tcgggaaata ccgaaaaact cggaccggcc aatgcgtgag ccgtatcgct
     4861 cacaacctgt tcagcatatc gctttcctag ctcaggccag tccatgagcc ctaccattaa
     4921 ggacggatcg atgacaggca gtggagggac aaccgaaaga atggtgactt tgacagagtt
     4981 cttgaaggga tgcgcgttca gccaggtgtg caaatggccc gcatcctcct ggttttctac
     5041 agccaggagc acgcgttgga caggacgagc tttgcctttc acgatcaagg tcggcactgt
     5101 cgcatggagc aggatgctat gggaaatgct cccgacaaac acctcggtca ctcggctgtg
     5161 atcgtgcgtg cctactgcga tgaggtcggc tttgaccgcg gcggcactgt cgagaatgaa
     5221 ggcggctgga tattcgacgt tacacatcgt ccgaatcgaa ggcgtctcag ttggcagcag
     5281 agtgcggcac cgctcaatag cttggcgacc ggcatccatt aagccttgtc gtaagtcgtc
     5341 gtatcctggc atattgatgg tccccgccat catgggagcg gtgagtaccc caaggtcgac
     5401 accatgaatg agcacgacct catcaggtcg gtataacaat ccgatctgtt ctacggcggc
     5461 aaaggcttcg tccgaccagt ccactcccac ggcaattcgc atcatcgccc tcctgtttga
     5521 actaggagcc gaattcccca tccatgctgg attcgag
//
LOCUS       DATIPF010000427         3113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_348444, whole genome shotgun sequence.
ACCESSION   DATIPF010000427 DATIPF010000000
VERSION     DATIPF010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348444"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(336..2516)
                     /locus_tag="VLL06_10655"
     CDS             complement(336..2516)
                     /locus_tag="VLL06_10655"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HSS31468.1"
                     /translation="MPYETVTELDQLLDRIKSYYADADLGLVRKAYEFSAKAHVGQLR
                     LSGEPYVQHPIAVAGVLTSLKTDVTAIVAALLHDTLEDTVATPDELEREFGKDVVHLV
                     DGVTKIGKITFRSYEEKQAENFRKMVLSMADDIRVVLIKLADRLHNMRTLEHLRPAKR
                     KEIAQETLEIYAPLANRLGIGWIKNELEDLCLKHLKPEVYETLRVRVAKRDEDRQQYI
                     DEVRRLVQKTMQENGLAGHVHGRPKHLYGIYQKMNKQSITFEEVYDLTALRIVTDTKM
                     NCYAVLGVIHSVWRPVPGRFKDYIAIPKSNLYQSLHTTVVGPKGEHVEFQIRTEDMHR
                     IAEHGIAAHWKYKEQGHVADRDSKAFGWLHQFVEWQEDLTDNRQFMDSVKLDLFHDVV
                     YVFTPKGIVKELPRGSTPVDFAFAIHTEVGDHCVGAKVDGKIVPLRHQLESGDTVEIL
                     TSPTQSPHKDWLKFVRTSRAKTKIKHWIKLEEQKRSIEIGRRLLESEFRRQGMAPARM
                     FKSPELHEAARQSGFDSTDELAAAVGFGRVSTSQVIVKLTVSSPGVQAPAQERPAARK
                     AVGGKSDSTGVQVKGSRDLLMQLSRCCHPVPGDRILGYITRGRGLTIHAVDCPNLEAL
                     DYDKERLVEVEWDSDTPSTHAVKMSIVAVDKTGVLANVSSAIAECHANISRAEITTRE
                     DQKAVLDFIVEVSDTRHLERVIKAVERVDGVITARRMRAWQERS"
     gene            complement(2532..3104)
                     /locus_tag="VLL06_10660"
                     /pseudo
     CDS             complement(2532..3104)
                     /locus_tag="VLL06_10660"
                     /inference="COORDINATES: protein motif:HMM:NF037628.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
BASE COUNT          661 a          950 c          815 g          687 t
ORIGIN      
        1 cgagaacgga cgacagctgc ccccaatatc ggcccaacga agacgcgctc tatgtcctgg
       61 aacttgcagc aggccgagct gagagtttga agctgcagcg aggatcgaaa cttcagttcc
      121 gactcccgta ggtctagcaa gctacggaaa atctattgac gcaggccagt aacctcacta
      181 atccgcgcat ctggaaaaac acgagaatac gacgcaggat gctcaaaaag tccgtcagca
      241 aggccgcagc gagcgaagag gcgaggcgta cgcttcggta cgttgagcct ctgaacgatg
      301 cgagaacgaa gctggcgggc tttttcagca tcctgctaag agcgttcctg ccaggcccgc
      361 atccgcctcg ccgtaatcac cccatccaca cgctcgacgg ctttgatcac ccgttcgaga
      421 tggcgggtgt ctgacacttc cacgataaaa tccagcacgg ctttctgatc ctcccgtgtc
      481 gtgatttctg cgcggctgat gttggcatgg cattctgcga tcgcggagga tacgttcgcc
      541 aatactccag tcttatcgac ggcgacgata gacattttca ctgcgtgcgt gctgggcgta
      601 tcggagtccc attcgacctc tacaaggcgc tctttatcgt aatccagcgc ctcgagattc
      661 ggacaatcga ccgcatggat cgtcagccca cgcccacggg taatataccc aaggatgcgg
      721 tcgccgggca cgggatggca acagcgcgag agctgcatca acaagtcccg agaccctttg
      781 acctgtacgc cggtgctgtc gctcttccca cctaccgctt tacgtgcggc gggacgctcc
      841 tgagccggcg cttgtactcc gggcgacgaa accgtgagtt taacgatgac ctgcgatgtc
      901 gacacgcggc caaagcctac tgccgcagcc aactcgtcgg tgctgtcgaa ccccgactgg
      961 cgagcggctt catggagctc cggcgatttg aacatccggg ccggtgccat cccctgacgg
     1021 cggaactccg actccagtaa acgacggccg atctcaatgc ttcgcttctg ctcttccagt
     1081 ttgatccagt gcttgatctt ggtcttcgca cgagaggtac ggacaaattt caaccagtcc
     1141 ttgtgcggcg attgggtagg agacgtcaga atttcgaccg tatctccgct ctccagttga
     1201 tgtctcagcg gtacaatctt cccatccacc ttggccccga cacagtggtc cccgacctcg
     1261 gtgtgaatcg cgaatgcaaa atcgaccggt gtcgaccctc tcggcaactc tttcacaatc
     1321 cctttcggcg tgaagacata caccacatcg tgaaagaggt cgagcttgac tgaatccatg
     1381 aactgccggt tatccgtcaa atcttcctgc cattcgacga attggtgcag ccagccgaag
     1441 gccttgctat cacgatcagc cacgtgtcct tgttctttat acttccagtg cgcagcgatg
     1501 ccgtgctcag cgatccggtg catgtcctcc gtgcggattt ggaattcgac atgctccccc
     1561 ttcggcccca ccaccgtcgt gtgcagagac tgatagagat tggactttgg aatcgcgatg
     1621 tagtctttga agcgtccggg cacgggccgc cagactgagt gaatcacccc gagcacggcg
     1681 tagcagttca tcttggtatc ggtcacgatc cgcagtgcgg tcagatcgta gacctcttca
     1741 aacgtaatcg actgtttatt catcttttgg taaatgccgt agagatgttt gggccggcca
     1801 tggacgtgtc cggccaatcc gttctcctgc atggtcttct gcacgagccg gcggacttca
     1861 tcgatgtact gctgccgatc ctcgtcccgt ttagccaccc ggacacgcaa tgtctcatag
     1921 acctcaggtt tgagatgctt gagacacaga tcttccagct cattcttgat ccatccgatt
     1981 ccaaggcgat tcgccaacgg cgcatagatc tccagcgtct cctgggcgat ctccttccgc
     2041 ttggccggcc tgagatgctc caaggtacgc atattgtgca gccggtcggc gagcttgatg
     2101 aggacgaccc ggatatcgtc cgccatcgac aagaccatct tgcgaaagtt ctccgcctgt
     2161 ttttcttcat agctccgaaa cgtgatcttg ccgatcttcg tcaccccatc gaccagatgc
     2221 acaacgtcct ttccgaactc ccgttcaagt tcgtcaggcg tcgcaaccgt gtcttccagg
     2281 gtatcgtgca gcagtgctgc gacgatggcg gtgacatccg ttttgagcga ggtcagaact
     2341 ccggcaacgg cgatgggatg ttgaacgtag ggttctccgg acagacgtag ttgcccgaca
     2401 tgggctttcg cagaaaactc gtaggccttg cgaacgaggc cgaggtcggc atcagcatag
     2461 taactcttga tccgatccag cagctggtcg agttctgtga cagtttcgta cggcataatt
     2521 cactcactga cttactcacg cacactcggt cgaacatcgc gaatgcgaag ctgaatccgg
     2581 tcacgtccgt tccagtgatt gagttccggc gtgaaggcca agtcgacggg agcacgggac
     2641 ggaatccccc gttccagcaa cgactacata ccgaagccaa tgctatctaa tggcagcgat
     2701 gccccctgcc gcaccgtcat tttcagatgt ttctcaccga cggtacgcga gtccatgact
     2761 tcgaggccgg ttaccgcaaa cgtgggttct ggatttccct cgccgaacgg atgcagcgaa
     2821 ccgatttcct ggatcaacag aaagttcact tcatctaaac gcacttcggc atcgacgttc
     2881 aacgtcggga ccttggcacc atcgtgggtc cacccagcca ctacatcgga gaatctacta
     2941 cgaaattcat cgatccgaga ctcgcgaaga gtcacgcctg cggcactcgg atgcccgccg
     3001 aacccctcca acatctcccg acaggcggcc aagccttggt agagatcgaa cccaccggtc
     3061 gtccgtgccg agcctttggc cacaccctga tcgtcgacag ccatgacaat ggt
//
LOCUS       DATIPF010000428         3887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1232824, whole genome shotgun sequence.
ACCESSION   DATIPF010000428 DATIPF010000000
VERSION     DATIPF010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232824"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VLL06_10665"
     CDS             <1..326
                     /locus_tag="VLL06_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSS31469.1"
                     /translation="PSEAMHALQAYHWPGNVRELENFIERAVILTQGPDLVVSLAELK
                     RTPTHTTQSGATTLEQAEREHILRSLRETNWTIGGPAGAAARLGMKRTTLQSKMQKLN
                     ISRPR"
     gene            550..1506
                     /locus_tag="VLL06_10670"
     CDS             550..1506
                     /locus_tag="VLL06_10670"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HSS31470.1"
                     /translation="MNESLQFLTQHGTLVLFVVVLSEQIGFPVPAFLFLIAAGALVGT
                     GQMSLGMAIGVAVLAAVMGDQLWYELGRRRGRRVLNWLCRISFEPSSCVSRTEEFFTR
                     HGVRSLIIAKFIPGLSTIAPPLAGIVGLRLPPYLMYNGLGTVLWVSSGIGVGYAFSDQ
                     LEQAVSVVAYLGPAMALILLGSVAVYVIAKALHRYRVERLVPRLTVQEVTEKIAAGEA
                     PVIIDLRSQAARREVPGIQGSLTLSVEDVIAGYRNLPRDRDVILFCGCPLDRSSIRAA
                     RKLREKGLTRVWPLVGGIEAWHAMTSTTGNIMGNSEGHTVTV"
     gene            1563..2549
                     /locus_tag="VLL06_10675"
     CDS             1563..2549
                     /locus_tag="VLL06_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31471.1"
                     /translation="MELKIPSQEQAYWGLRAMKTVALADGVLDDSELHLLASIQRIFG
                     TTHEVDQLSPITPADLALGFSDPQLRQQLVQGLIVMSLIDGKANAQETHLVEEFAQSL
                     EVSAPEVKDLRNVLKGEIFRLRLDLARRFWLRDKVKDIWNTKGIRGLYAFMRGMIGRN
                     EDLALAARYQAMEQYPSGSLGRAYWDYCRENGFALPGEKGGAAEPILFHDCAHILSGY
                     GTDPEGEVQVACFSAGFQRRDPWLFVFFVLLQFHVGIRMTPITKARTGFFNPEKAMIA
                     IRRGAAMNVDLNNGWDYWPVMGEQVEELRRQYSILPAEAFLPSSRDAIAIEA"
     gene            2620..3342
                     /locus_tag="VLL06_10680"
     CDS             2620..3342
                     /locus_tag="VLL06_10680"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSS31472.1"
                     /translation="MTTANRYLTSWENYWSTLRGTPGEIFWDADPAHAAQQDLGLFQG
                     YADPQLPLLDLGCGNGTQTRFLADHFSTVIGTEISPAAVEIAQTRNSAPNVSYRVLDV
                     LCLEDAQALHEEIGDANLYMRTVLHQLSPADHAAAIRSIERLLGANGILYMIELSSAA
                     ELYFAQLIEQHGPPPGLARVFQHGRTPGMLNENDLTMLFPPERFTVCSTGQSQVHTVH
                     TLLTGEVVKVPAFYAVLRQRQR"
BASE COUNT          839 a         1006 c         1135 g          907 t
ORIGIN      
        1 ttccgtcgga ggccatgcac gcgctccaag cctatcattg gcccggtaac gtgcgggaac
       61 tggagaattt tatcgagcga gcggtgattc ttactcaagg gccggatctc gttgtgtcgc
      121 tcgccgaact gaagcggacg ccgactcata ccacacagtc cggcgccacg acccttgaac
      181 aagccgagcg cgaacatatt ctcaggtcac tccgcgagac gaactggacc atcggtggcc
      241 ctgctggtgc cgctgccagg ctcggaatga agcgaacgac cttgcaatcc aagatgcaga
      301 agctcaacat ttcccgacct cgatagcctc gccgatcatt cggcatctgc cgggatatcg
      361 gcagcctttt cccgtcacag ccgtctcgtt catctccatt cattcaactc gagtgagcgg
      421 gatcttcact gtttttccac acttgggcat tttgcccaga cagacaactt ctgtggaacg
      481 atcattgcca tagtccgaga acaacatgag acactcgcat caaacttctt ttggttgatg
      541 agggccagta tgaacgaatc acttcagttc ttgacgcaac acgggacact cgttctcttt
      601 gtcgtggttc tgtcggaaca gatcggattt cctgtccctg cttttctgtt tcttatcgcc
      661 gccggtgcat tggtgggcac tggtcaaatg tcgttaggga tggcgattgg ggtagcggtc
      721 cttgcagcag tgatgggaga tcaattgtgg tatgaacttg gccgccgtcg aggccgtcga
      781 gtcttgaact ggctctgccg tatttcgttc gaaccaagtt cctgtgtgag tcggacagag
      841 gaattcttta cgcgtcatgg agttcgctcc ttaatcatcg cgaaatttat cccgggtctg
      901 agcacgattg ccccaccgct tgccggaatt gtaggactcc ggttgcctcc gtacctcatg
      961 tacaacggct tggggacggt cctctgggtt tcttccggga ttggggtcgg ctatgcgttc
     1021 agcgatcaac tcgagcaggc ggtatccgtg gtggcctatc ttggtccggc gatggcgctg
     1081 atcctgctcg gaagcgtcgc ggtgtatgtc atcgccaagg ctctgcaccg gtatcgcgtg
     1141 gaacgtctgg tgccacgatt gactgttcag gaggtgacgg agaaaattgc ggcaggagag
     1201 gcccccgtca ttatcgacct gcgttctcag gctgctcgtc gggaagttcc gggcattcaa
     1261 gggtcgctga cgttgtcagt ggaagatgtc attgcagggt atcgcaatct tccccgagac
     1321 cgtgacgtga ttctcttttg cggctgcccg ctagatagat cgagcatccg ggctgcgagg
     1381 aagcttcgcg aaaagggtct gacacgtgtg tggccgctcg ttggtggaat agaagcctgg
     1441 catgcgatga cgtcgacaac cggaaatatc atggggaatt ctgagggaca caccgttacg
     1501 gtttgaccgg tctactgtcg ggcagagcca cgacacaaga aaggaaagta agagggaaga
     1561 ccatggaatt gaagataccg agccaggaac aggcctattg ggggttgcga gcgatgaaaa
     1621 ctgtggcgct ggcggatggg gtactcgatg attcggaact gcatctgctg gcatcgatcc
     1681 aacgtatttt cggtacgaca catgaggtgg accagctttc tcctattaca ccggcggatc
     1741 tggccctcgg attttccgat cctcagctga ggcagcaact ggtacagggt ttaatcgtca
     1801 tgtcccttat tgacggaaaa gccaatgcgc aggagacaca cctcgtcgag gaattcgcgc
     1861 agtcgttgga ggtcagtgct cccgaggtga aggacctccg gaatgtgctc aagggggaga
     1921 tctttcgact gcgcctagac ctggcgcgcc gattctggtt gagagacaag gtcaaagata
     1981 tctggaacac aaagggaatc aggggcctct atgcgtttat gcgcgggatg atcggaagga
     2041 atgaggatct ggcactcgcc gcccgctatc aggccatgga gcagtatcct tccggttcac
     2101 tgggacgggc ctattgggac tattgccgcg agaacggatt cgccctgccg ggggaaaaag
     2161 gtggtgcggc ggaacccatt ttgtttcacg attgcgcgca catcttatcc ggctatggga
     2221 cggacccaga gggagaggtt caagttgcct gtttcagcgc aggtttccag cgccgcgatc
     2281 cctggctctt cgtctttttt gtcctgctgc agttccatgt gggcattcgc atgactccca
     2341 tcacgaaagc ccgcaccggc ttcttcaacc ctgagaaggc catgatcgcg attcggcgcg
     2401 gggcggcgat gaatgtggat ctcaacaacg ggtgggacta ctggccggta atgggagaac
     2461 aggttgagga gctgcgaaga cagtacagca tcctcccggc agaggcgttc ttgccgtcaa
     2521 gtcgtgacgc gatcgcgatc gaggcctaat ggacaacatc ttcccgagtg cggatgtgtc
     2581 gttccgatac tgataatcac acaatcatag gagcaagcga tgacaacggc gaatcgttat
     2641 ctgacatcgt gggagaatta ttggagcacg ctgaggggaa ctcctggtga aatattttgg
     2701 gatgccgatc ccgctcatgc cgctcaacaa gatttggggc tctttcaagg ctatgccgac
     2761 ccccagcttc cgttgcttga tctaggttgt ggtaacggca cacaaacgcg ctttcttgct
     2821 gaccattttt ccacggtgat tggcactgaa atttcccctg cagcggtcga gattgcccaa
     2881 acgaggaata gtgctcccaa tgtctcgtac cgtgtgcttg acgtgctgtg ccttgaggat
     2941 gcccaagccc tccacgagga aattggcgat gccaatctct acatgcgaac agtgttgcat
     3001 cagctgtctc ctgcggatca cgcggccgcg atacggagta tcgagcgact acttggagcg
     3061 aacggaatat tgtatatgat cgaattgtca tcagcggctg aactctactt tgcccaattg
     3121 attgagcagc atggaccacc tcccggtctt gcacgggtgt tccagcacgg aagaaccccc
     3181 ggcatgttga atgaaaacga tttaaccatg ctatttccac ccgaacgttt tacggtgtgt
     3241 agtactggcc agagtcaggt tcacaccgtg catactctgt tgactggtga agtggtgaag
     3301 gtgccggcat tttatgccgt tcttcggcaa cggcaacgat gagcgtcctt gcgtctggaa
     3361 ttgatgcgaa tcccggtgag gtgaaagggt gtgtgttatt ccggcaaaaa gtcgagggag
     3421 tgaaggagaa gtgcgactca tctgtcggag ctggcggacg taacagccgg atggaatccg
     3481 atgctcgaac agcctacgcg gctctctgta tgtagcaatc tgtcgcgacg atctgtcccc
     3541 ttcaaacgct ctaagcgcgg caggattttg ccagcttgac ccgcctgccc ggcgatgttg
     3601 acgctgtcaa tatggcgccg cacgatgcct tcgagcaggt agcgaggcga gcacacatga
     3661 acgctcacat gtatgacgaa agagccaagc agactgatgc tgcctccttt cagatcctgc
     3721 cgatggttcg gcgagtgccg gtatagcggc aggacatcgt cgtcagccag tccatcggat
     3781 gaacaacacg ggaaaatata cgtgcttggt caatacgtta gctggtcatg ggcaataggt
     3841 attgctggaa cggcctttgc cattactaca gcttgaaggg aagcaga
//
LOCUS       DATIPF010000429         6820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1495060, whole genome shotgun sequence.
ACCESSION   DATIPF010000429 DATIPF010000000
VERSION     DATIPF010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495060"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VLL06_10685"
     CDS             complement(<1..183)
                     /locus_tag="VLL06_10685"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HSS31473.1"
                     /translation="MTPAATSDEAGAPLLDEDYLILSTIHSAKGQEWKSVFVLNVVDG
                     CLPSDLGTGTTAEIEEE"
     gene            203..877
                     /locus_tag="VLL06_10690"
     CDS             203..877
                     /locus_tag="VLL06_10690"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSS31474.1"
                     /translation="MRTRVPESPAFETIRLAGGLASSPIRDVFTTQRLAVAKVVCLVW
                     LHGVAFYLLYVYMTTYLVTVTSVPLATVLILNTGCMALLALLIPLMGAWSDRIGQAPL
                     LMTGATGIALLSYPSFLWLTSNNLPRMIAAQVLLTLLVACYMGPFFAAVTNLFPTAQR
                     YTGLSIGYNTGAALFGGTAPLIASLLIEWSGNILAPAFYLTFCATVSFAVTLTLRNRE
                     PAGSAH"
     gene            988..1641
                     /locus_tag="VLL06_10695"
     CDS             988..1641
                     /locus_tag="VLL06_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31475.1"
                     /translation="MPTSRGENLTPLLLSLLLITIVSLFCPSVTQAVDFGNEVSVWVE
                     QGRVIATSLVSGRREIPLDAQEAVIDSGTSGINAVVVTSRRLLGFSSRTFTWAKKDRD
                     LNEKVLDRRVLPTFSVVRTDKHLYGFRGANGIWLDETLGVRESVKMTRTTDYGAVFVT
                     NERLVGFTSLLGDFSSKALGIHEHVTRLDNENGLILVTTSTRILVFGSRLSGWEEFE"
     gene            complement(1708..2436)
                     /locus_tag="VLL06_10700"
     CDS             complement(1708..2436)
                     /locus_tag="VLL06_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSS31476.1"
                     /translation="MATVSEIAPDVFRISTFVREFDLQFSQFLVRDDEPLLFHTGPRA
                     LFTETRDAVATLLDPASVKWIGFSHLESDECGTLREWQQLAPKSLAVCSVVGKLVSID
                     DFMTVRPALGMKDGELLNTGKYRFQFLHTPHVPHCWEAGLLFEETQRTLFCSDLFHQN
                     GDVEPATHSDVLGRCRQVLVEYQQGPLANYMPYTTLTEPTLHRLAELQPKTLATMHGS
                     AFVGDGARALRDLAAIFKDVLGPK"
     gene            2671..3273
                     /locus_tag="VLL06_10705"
     CDS             2671..3273
                     /locus_tag="VLL06_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31477.1"
                     /translation="MWCRHGIWLLMAGMFWGCALLNGTSQPNSAPLFQADATDTATLE
                     AVHREQVALLSTCTQHRSCDQVHFTRAMLALFENREAAAASFQQAIAAAPNGPFSDSS
                     ALWIRFLSNPTLYSASTDRPNRALLEVMKGTVRVWLGRQRVAGDTVRGLEPTAYNLRR
                     QIRTRDKRIAELTDQLHALKQIDLDSHGAGKLGPPRTPSK"
     gene            3454..4659
                     /locus_tag="VLL06_10710"
     CDS             3454..4659
                     /locus_tag="VLL06_10710"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HSS31478.1"
                     /translation="MHRAVGLNSQELKPNTSILCLTLILWLTFLIRQTVGHRIQLRSK
                     TFATEAVPDVSAMRLLVLSQIADWDFGPRVKAGGHEIVAWVRPIWARGERYSEGAQTL
                     RTTFRTIFSGPEPIRTIPPKFDTWTWLEREKIPRIPCPNVNAPKFIEYVKGLNIDLIV
                     SSIFPQILKATILRIPRLGAINCHPSLLPRYAGPQPEFWMLKNGESLAGITVHMMTEG
                     IDAGDIVAQQELTVGEHENIGQLVQRQHHAAARLLTETVNAMAQGTIERKPQDIAERS
                     YFGKRKATDIMVDWNGSARQILNLLRALQPYEPVTASLNGHTIKIYEAQLQEKAASGG
                     VPGQIMAKQPGKLLVQTGKGSLEIQSYEIAPFHGWINRLLQKVLMPSVGYRFDIFPTS
                     SGSTPQSTS"
     gene            complement(4781..5509)
                     /locus_tag="VLL06_10715"
     CDS             complement(4781..5509)
                     /locus_tag="VLL06_10715"
                     /EC_number="1.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004128 - cytochrome-b5 reductase
                     activity, acting on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-cytochrome b5 reductase"
                     /protein_id="HSS31479.1"
                     /translation="MELVTRIKTKEPTSYELTAIHPATHDTKTFRFGLPDHATLDMLP
                     GDFLYVHASINGRAVKRPYTPSSLPGITGHFDLTVKRYETGLISKYLHEQKVGDPVLM
                     SGPNQGGHWMDGMAKKVGFVAGGTGITPMISIIRWILAQHLDVELFLVFANKTEADII
                     FREEWSRNVREHSNFHCYHILEQPPAGWSQGSGRITEDVLRRQLPSPNSETVIFLCGP
                     PPMVDHLEASLKGLGYSEQAIILP"
     gene            5774..6556
                     /locus_tag="VLL06_10720"
     CDS             5774..6556
                     /locus_tag="VLL06_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HSS31480.1"
                     /translation="MARSTLRIAFLHLAPVPGDLIQNRRAIEAAIIRAAQTGATWILT
                     PELAICGYTFADTLGTDWIEPQPDQWVSTLCQLTARLRITLFLSHPERDRKTDTLYNS
                     LFVITPDGCVVGSHRKINTLRVGSEAWSSPGNRVEPIALSAVGRVGLLICADAYTPAI
                     ATSLKAHGAQILVSSAAWAPGLHGPNGEWERCTKDTGLPLLVCNRTGPDRTLNFTQAE
                     SVVAQDGKRLLAFQSARSTIFIIDWNLATNRLASTRFQQIAL"
BASE COUNT         1498 a         2065 c         1800 g         1457 t
ORIGIN      
        1 ctcctcctcg atctcggccg tcgtccccgt ccccagatcc gacggcaggc agccgtccac
       61 cacgttcagc acgaacaccg acttccactc ctgtcccttg gcggagtgga ttgtcgaaag
      121 aatgagatag tcctcgtcga gcagcggcgc gccggcctcg tcgctggtcg ccgcgggcgt
      181 cattgccgtg gtgggatggt acgtgcgaac cagagttccg gagtcgccgg ccttcgaaac
      241 aatccgcctg gcaggcggac tcgcatcttc ccctatccgc gatgtgttca ccacgcagcg
      301 gcttgccgtg gcaaaagtcg tctgcctcgt gtggctgcat ggcgtagcat tctatctctt
      361 gtatgtctat atgacgacct acctggtcac ggtgacgtct gttccactgg ccactgttct
      421 cattctcaat acaggctgca tggcgctcct cgctctgttg attcccctga tgggtgcctg
      481 gtcggatcgg atcgggcagg ctcccctgct gatgacagga gccacgggga tcgcgctcct
      541 gtcctatcca tcctttctct ggctcacgag caataacctg cccagaatga ttgctgcgca
      601 agtgcttctt acgttactgg ttgcctgtta catgggacca ttttttgctg ctgtcacaaa
      661 cctcttccca accgcgcaac gctataccgg cctctcgatc ggctataaca ccggggcagc
      721 cttgttcggc ggcactgctc cactgattgc ctcgctcctc attgaatgga gcggtaatat
      781 cttagcgcct gccttctact tgaccttctg cgcgactgtg tcctttgcgg tcacgctcac
      841 gcttcgaaat agagaaccgg cagggtcggc tcactagcca gatgagtggc atcagcgaag
      901 accagccaga tcggacgctc ggatcagttt cggcccatgg gtaccgaaac accataccgt
      961 accgacatgc ggtagaatct ccaggccatg ccgacgtctc gtggagagaa cctgacacct
     1021 ctccttctca gcctgctgct gataacgatt gtctcgctct tctgtccaag cgtcactcaa
     1081 gcggtcgact ttggcaacga ggtctccgtc tgggtcgagc agggccgggt catagctacc
     1141 agtttggtga gcggccggcg ggagattccc ctcgatgccc aagaggccgt cattgactca
     1201 ggtacaagcg gcatcaatgc cgttgtcgtg acctcgcgcc ggctgctggg cttttctagc
     1261 cgtaccttta cctgggccaa gaaggaccgc gatctcaatg aaaaggttct tgaccgacgg
     1321 gtgttaccca ccttcagcgt ggtgagaaca gacaagcatc tgtatggatt tcgcggcgcg
     1381 aacggaatct ggctggatga aaccctcggg gtacgagaat cggtcaagat gaccaggaca
     1441 actgattatg gtgcggtatt cgtcacgaac gaacggcttg tgggctttac ttctctgctc
     1501 ggcgacttct cttcaaaggc tctcggcatc cacgagcacg tcacgcggtt ggacaacgaa
     1561 aatggactca tcctcgtcac caccagcaca cgcatactcg tgttcggcag ccgcttaagc
     1621 ggatgggaag aattcgagtg agatgtccgc ccgtaaggct gcgtcaaacg agacgcgaat
     1681 ggctggtgct gccctcagat tctcgtgcta tttcggcccc aagacatcct tgaatattgc
     1741 agcaaggtcg cgaagcgccc gggcgccatc tccgacaaag gcagacccat gcatcgtcgc
     1801 caaggtcttc ggctgtaatt cggccagccg atggagcgtc ggctcggtga gtgtcgtgta
     1861 cggcatataa ttggccaacg gcccttgctg atactccacg agcacctgtc ggcagcggcc
     1921 cagcacgtcc gaatgcgtgg ccggctcaac atccccgttt tgatgaaaga ggtccgaaca
     1981 gaacagcgta cgctgggtct cttcgaacaa caaccccgct tcccaacagt gcggtacatg
     2041 cggcgtgtgt aggaactgga aacgatactt tccggtgttc agcagctcgc cgtccttcat
     2101 ccccaatgca ggcctgaccg tcatgaaatc gtcgatgctc accagtttgc cgaccacact
     2161 gcatacagcc agagacttcg gcgcaagctg ctgccactca cgcaaggtac cgcattcatc
     2221 cgattcaaga tggctgaacc cgatccactt caccgaggcc ggatcgagca acgtggccac
     2281 agcgtcccgc gtctccgtaa aaagcgcccg cgggccggta tgaaacagca gcggctcgtc
     2341 gtcacgcacg agaaactgac tgaactgcag atcgaattcc cgcacgaatg tcgaaatcct
     2401 aaatacgtca ggtgcgattt ccgacacagt ggccatgagc acctccaaag gtggcggaaa
     2461 agagaccgta ggctagcgcc aatgttttct tgagtcaatg caaacaggtt gccagagttc
     2521 atgcggaaac cgcttcgcac ggacgaatcg atccaaatga ggcgaatgtc caagctttta
     2581 cccctttctc ctgtctggcg agatccaaac cgtctgctaa gcttgtctga acacaggcac
     2641 cgagatctcg gtgcaaaaag gggtatcacg atgtggtgtc gtcatggaat ctggctatta
     2701 atggcgggga tgttctgggg gtgtgcgtta ctgaatggga catcgcagcc caactctgca
     2761 ccattatttc aagccgatgc caccgacaca gccaccttgg aagctgtaca tcgagaacag
     2821 gtagcgctgc tgagcacctg cacccagcac cggtcctgtg atcaggtgca tttcacacgt
     2881 gcgatgcttg cattgttcga gaatagagag gcagcggcag cctcctttca gcaggcgatt
     2941 gccgccgcac cgaatggtcc attctcggat tcgagcgcac tctggattcg gtttctttcc
     3001 aacccaaccc tctactctgc ctcgaccgac agacccaata gggcactcct ggaggtgatg
     3061 aagggaacgg tacgcgtatg gttgggacga cagcgcgtcg caggggacac tgtcagaggc
     3121 ctggaaccca ctgcctacaa tcttcggcgg cagattcgta cccgcgacaa acggattgcg
     3181 gaattgaccg atcagcttca tgccctgaag caaatcgatc tggactcgca cggagccggc
     3241 aagctgggtc ccccgagaac tccgtccaag taattacttc tgaggcaccg ccactcttgg
     3301 ccacatcgcc cgtcccatac ctatactccc ggcaagcccc acctgcctcg ccacccgttg
     3361 ttcgaatcat ccgcatccct gcaccacaaa gcataccgtc cagctcttcg catgacagga
     3421 ttattgctcc catgctacag tctcctgcac agaatgcacc gggccgtcgg attgaactca
     3481 caggagctca agccgaacac ctcaatacta tgcctgaccc tcattctttg gctgactttc
     3541 ttgatccgcc agaccgtagg gcacaggatc cagctacgat ccaagacatt cgccacggaa
     3601 gcggtccctg acgtatccgc catgcgtctc ttagtccttt cgcaaattgc cgactgggac
     3661 tttggtccgc gggtgaaagc gggcgggcat gagattgtcg cctgggtacg ccccatatgg
     3721 gcgcgaggcg agaggtacag cgagggtgct cagacgctga gaaccacgtt cagaacaatc
     3781 ttttccggac cagaaccgat ccggaccatt ccaccgaagt tcgatacctg gacgtggctt
     3841 gaaagagaaa agatcccacg tattccctgc cccaatgtca atgcgccaaa atttattgaa
     3901 tatgtgaagg gtttgaacat cgatctgatt gtatccagta tctttcctca gattttaaag
     3961 gcaaccattc tccgcatccc tcgtttaggc gccatcaatt gtcatccttc tctccttcct
     4021 cgttatgcag gaccacagcc ggaattttgg atgctgaaga atggagaatc gcttgcgggg
     4081 atcaccgtcc atatgatgac ggagggcatt gatgcagggg atattgtcgc ccagcaggaa
     4141 ctgactgtcg gagagcacga gaatattggg caattggtgc agcgacaaca ccatgcagcg
     4201 gccaggttac tgacagaaac cgtcaatgcg atggcgcaag ggaccatcga gcgcaagccg
     4261 caagatattg cggaacgcag ctactttgga aagagaaaag cgaccgatat catggtcgac
     4321 tggaacgggt ccgcgagaca gattctgaat ctcttgcgcg cgctacaacc ctatgaaccg
     4381 gtcacagcct ctctcaacgg acacacgatt aagatttacg aggcgcaact tcaagagaag
     4441 gctgcatcgg ggggagttcc cggacaaatc atggcgaagc aaccggggaa actccttgta
     4501 caaaccggca aggggtctct agaaatacag tcctatgaaa tcgccccgtt tcacggttgg
     4561 atcaatcgtc ttctccaaaa ggtcctgatg ccgtccgtcg gctaccggtt cgacattttc
     4621 cccacatcat cgggctcgac acctcagtca acatcctgag caccctttcc aaacccaatg
     4681 aaaagaagcc gtgcaccctc aatgttccgg tgtgtcaacg aggggaacct gctgggaatt
     4741 tccgagacct catgcaccga caaggctgac ccgaaccaat ttatggcaat atgatcgcct
     4801 gctctgaata gcccagcccc ttgagggagg cttccaggtg atcgaccatc ggcggtgggc
     4861 cgcagagaaa gatgaccgtc tcggaattgg gagagggaag ctgccggcga aggacgtctt
     4921 ccgtgatccg tccgctaccc tgcgaccatc cagcaggcgg ctgttcgagg atgtggtaac
     4981 agtggaagtt tgaatgttcg cgcacattcc gactccactc ctcccggaaa ataatgtctg
     5041 cctcggtctt gttcgcgaag accaggaaca actcaacatc caggtgttgc gcgagaatcc
     5101 aacgaatgat cgagatcatc ggcgtgatcc cggtaccgcc ggccacgaac cccaccttct
     5161 tcgccatgcc gtccatccaa tggccgccct ggttcggtcc gctcatcagc accgggtccc
     5221 cgaccttctg ctcatggagg tacttcgaaa tcaggccggt ttcgtagcgc ttcacggtca
     5281 agtcgaagtg gcccgtgatc ccaggcaacg acgacggcgt atagggtcgt ttcaccgccc
     5341 ttccgttgat gctggcatgg acgtacagaa aatcgcctgg cagcatgtct aacgtcgcat
     5401 gatcaggcag accaaaacgg aatgtcttcg tgtcgtgagt ggcgggatga atagccgtga
     5461 gctcgtagga ggtcggctct ttggttttga tacgtgtgac cagttccatc gtcatccctc
     5521 catcgtgcgg gcgcaatcat acaatcacga cgcgatggtc gcaacaggat gatgtgcgat
     5581 aaataaacca tgagccacga ggaaacgtgt ctccaacggg cagacagaac gagtccgagc
     5641 gggctgccat gtcccattca ctgagttgcg aggttgcata gtggcatcag tcactcagcc
     5701 ttcaatcggg aaagcctcgt cgagaatagg ccaggatctg tgctagagtc cggcccggat
     5761 agagagagac tccatggccc gatcgacgct gcgcatcgcc ttcttgcatc tggccccggt
     5821 gccaggagat ctcatccaga atcgtcgtgc gatcgaagcc gccattatcc gcgcagcaca
     5881 aacaggcgcc acatggatcc tcactcccga actcgcgatc tgcggctata cctttgccga
     5941 tacgctcggc acagactgga tcgaaccgca acccgatcag tgggtgtcca ctctgtgcca
     6001 gctcactgct cggcttcgaa tcacgctctt cctctcgcat cccgaacggg atcgcaagac
     6061 cgatacactc tataattcac tcttcgtcat caccccggac ggatgtgtcg tcggatccca
     6121 tcgcaagatc aatacgcttc gcgttgggtc cgaagcctgg tcgagcccgg gcaatcgtgt
     6181 tgaaccgatt gccctctcag ccgtcgggcg cgtcgggctc ttgatctgcg ccgatgccta
     6241 tacccccgca atcgccacta gcctcaaagc ccacggggct cagatccttg tgtcatcagc
     6301 agcctgggcg cctggactgc acggtccaaa cggcgaatgg gaacgatgca ccaaagatac
     6361 gggattgccg ttactcgtct gcaatagaac cggtccagac cgtaccctga acttcacgca
     6421 ggccgagagt gtggtggctc aagacggaaa acgcctgttg gccttccagt cggcccgctc
     6481 caccatcttc atcatcgact ggaatcttgc aaccaaccgc ctcgcctcca cgcggttcca
     6541 gcagattgcc ttgtagccca tcaacgaacc aagggtcagg ctaccctctc ctttccttcc
     6601 ccgcttgtcc tcacactctc tcaagttgtc aggctcatgt gtcttcaact tcgcgcgtcg
     6661 atcgatgaat atccaatgaa ccttccaagc tcgatcgtat ctctctcttc agagggtagc
     6721 ctggtcgatc ctcaaatgcg cgcgtcgaac gagcatcttc cgagcatgcg cgttccgcga
     6781 gcaggaggac gaccaggcta ccctctctcc cctattcgaa
//
LOCUS       DATIPF010000430         7042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_966477, whole genome shotgun sequence.
ACCESSION   DATIPF010000430 DATIPF010000000
VERSION     DATIPF010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966477"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VLL06_10725"
     CDS             <1..369
                     /locus_tag="VLL06_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31481.1"
                     /translation="ADKPGTLAGLLAPLRDAGVNLMAVHAFPRNRRTQVDVVPEDPIS
                     FKNVANVHQLKMQGPKVCLLVEGDDRAGALADVTDRLSSAKVNLTAVTGLSAGQGRCA
                     AILWVGPRDMKKATKIFGLR"
     gene            complement(424..738)
                     /locus_tag="VLL06_10730"
     CDS             complement(424..738)
                     /locus_tag="VLL06_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31482.1"
                     /translation="MKKTWIELGTISLLWTALAVVLTSAAATTFTGAIVGIDQAQRTM
                     IFQTVDGQTWALALSDSTMLKQEQVAKGDRVRIEVDLSGKVTKIMKITEPPSSVRTDL
                     IP"
     gene            complement(867..1493)
                     /locus_tag="VLL06_10735"
     CDS             complement(867..1493)
                     /locus_tag="VLL06_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31483.1"
                     /translation="MVLSRRNIVVFIAAATLMACVAFLSEPHHVTEADRIIDGSRVFL
                     LYQMTIRGEEVSEAPHFAQFVQGRHQFLPALERVVKGKKTGDTLKVVLSEDESFGTYD
                     ATKKKTVPRTELPGEVKEGDILQDGAGNPAIVTELSDRSAVIDYNHPWAGKSFIVKIA
                     ILRVDDPGTMDFTHPPGPRMMVKHQPRADEFREMGMGSEVLIAYTGSV"
     gene            2012..2938
                     /locus_tag="VLL06_10740"
     CDS             2012..2938
                     /locus_tag="VLL06_10740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HSS31484.1"
                     /translation="MRAIRLHQVGGPESLRDEEAPKPSPNDDQVLVQVSATAITPTEF
                     AWYPTFHTLDGGARPFPFILGHEFSGVVAAIGPDSDGVRIGDSVYGLNDWFGDGAQAE
                     YCLTVPGNLAPKPTSLVDSLAAAVPISALTAWQALVDRAQLSEGQRVLIHGGAGGVGS
                     FAVQLARYKKAHVIATVSAVNANFVTALGADEVIDYRTTPFEAVVRDVDVVLDTVGGE
                     TRDRSWEVLKKGGRLVTIAADAERFSDPRVRDAFFIVEPNRVQLIEISSLIDAGVIRP
                     VVGAVFPMEDVQHAYEQKPLRGKHVLYISGTS"
     gene            3400..5877
                     /locus_tag="VLL06_10745"
     CDS             3400..5877
                     /locus_tag="VLL06_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31485.1"
                     /translation="MRARIIALLCFALLVSCDTLPSLKQSVGISQDVRDEARLVNRSG
                     MSFPAADENYFGDMDGGAALSPEEVKGRNTWLVWSGGNDRLWNELSTRSFGTLDFLKT
                     LSSHPNLKSTRDTRWYYLGLVNEPCFEQATGPDPDRFGLWLDKRIVGPGCPPDPFENE
                     AKYPGVKIGARGKNLPVGSFYGYASGIVGMRLFPNPDFDEEAAKKWDAKRYYEDPTYY
                     LSKDLVKPYRVGMSCAFCHVGPNPVMPPENPEHPEWKHLSSNVGAQYFWIDRIFSWEA
                     DETSFPYQLFHTSRPGALDTSLVSTDYINNPRTMNAVYTLGPRLEQAKRWGQEKLSGG
                     NLNNKQLNDYVAEGPLTTFFQPPDIVLTPRVLKDGSDSVGALGALNRVFLNIGLFSEE
                     WLRHFNPLVGGKPITPIQIIDARNNSSYWEATEARTPNLALFFLKTTAPHKLKDAPEG
                     DQFLTEDVSLLLQGKEVFAERCARCHSSKLPVPAVGVDPHGCAGSGYLECWKKYWQWT
                     KTEEFKQKMRAIVLAEDFLEGNFLSSDLRIPVTLLETNACSPLATNAIRGNIWDNFSS
                     ESYKDLPSVGEITVHHPITGEPWSYVMPAGGVGYTRVPSLISLWSTAPFLLNNTVGRF
                     NPSPSVKGRMESFNNSIEQLLWPEKRDKDLILGDKVPGVIDRTTARSYLRVPNGYLPD
                     FLQGVVELEAKVLPDVQLLPPIFKEGGIEVGPIPKGTPVNLLTNLNVLPDDAGWIDRL
                     MHKRKIANLLIKIKGALNALPEHASDEEARKVFHDKGLVKPLLELSKCPDFVVNRGHY
                     FGTDFFKEEPGLTDEKKKALIAFLKTF"
     gene            5906..>7042
                     /locus_tag="VLL06_10750"
     CDS             5906..>7042
                     /locus_tag="VLL06_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HSS31486.1"
                     /translation="MAEISRRVFLKLAAATGITLSLGGCSLLGPSRSIGERCAGADTA
                     QPAESEYDYIVVGSGAGGGPVAANLANAGYRVLLLEAGGKEENANYVVPAFHPLSTED
                     QDQQWNYFVRHYANDNRQNKDSKRDKAGRGIWYPRAGTLGGCTAHNAMITVCPHESDW
                     DRIAALTGDASWSSEKMRRYFQLVERCQYSSRPLDFVTGGRHGFQGWLSTERPTQKTL
                     EAVVKNDPQLRHILTAVALTTLEDLPGSIPEAILLRAIPKLFSAALREDINHMDSITR
                     RPEGLYITPLATNGGRRAGVRDRIREVQERCPNQLVVKTNVLVTKILLEKLRTPHGEQ
                     SRAIGVECREGAHLYRADPRVGADMPAVQEFRAKQEVILSAGAFN"
BASE COUNT         1539 a         1893 c         2015 g         1595 t
ORIGIN      
        1 gccgataaac cggggacctt agccggtctg ctcgctccgc tgcgtgacgc gggagtgaat
       61 ctgatggccg tgcatgcctt tccccggaat cgccgcacac aggtggatgt ggtgccggaa
      121 gatcccatct cgttcaagaa cgtggcgaat gtgcatcagt tgaaaatgca gggacccaaa
      181 gtgtgtttgc tggtggaagg cgacgaccga gcgggagcgt tggccgatgt gactgatcga
      241 ctgagttcag cgaaggtcaa tctgacggcg gtgaccggtc tcagcgccgg tcagggacgc
      301 tgtgccgcca ttctatgggt cgggccgcgg gatatgaaaa aggcaaccaa aatcttcgga
      361 ctgagatagc gacgcagatt gccaccctca gtccctacgc agctcatgcc actcactatg
      421 cattcacgga atgaggtcgg ttcgaacaga ggacgggggc tcggtaattt tcatgatttt
      481 agtgactttc ccgctcaagt cgacctctat cctgacccga tcgcccttgg cgacctgctc
      541 ctgtttcaac atcgtggaat ctgacagcgc gagcgcccag gtttgtccgt cgaccgtctg
      601 gaatatcatc gtccgctggg cttgatcgat accgacgatc gcaccagtaa aggtggtggc
      661 ggccgcactg gtaaggacca ccgctagtgc ggtccagagc agactgatag ttccgagttc
      721 gatccaagtt tttttcatgg tgcacctcgg caataccgct gtgcttgtgc gctgtcctcc
      781 gccagccgtc caccgcgtcc cagaatgaga gagcactatg gtgaggggaa agaggagaga
      841 gagttttgcc tatcgaagag gtcccgtcag acagaacctg tataggcgat cagcacttca
      901 ctccccattc ccatttctcg gaattcgtct gcgcgcggct ggtgcttcac catcattcga
      961 gggccgggcg gatgcgtgaa atccatcgtt ccggggtcat cgactcgcaa gatcgcgatc
     1021 ttcacgatga atgacttacc ggcccacgga tgattgtaat cgattactgc cgaacgatct
     1081 gatagctcgg tcacaattgc cgggttaccg gcaccatctt gaaggatgtc tccttccttt
     1141 acttcgcccg gcagttctgt cctcggaacg gttttctttt tcgtcgcgtc atatgtgccg
     1201 aagctttcgt cctcggaaag gacgaccttc agcgtatctc cggttttctt gcccttcacc
     1261 acccgttcca aggccggcag aaactggtgt cgcccctgga cgaactgagc gaaatgtggg
     1321 gcttcggaca cttcctcccc acgaatagtc atctgataca agagaaagac acgggaaccg
     1381 tcgatgattc gatcagcctc cgtcacgtgg tgtggctccg agagaaacgc tacgcatgcc
     1441 atcaaggtcg cggcagcaat gaacaccact atatttcgac gagataaaac catgactcac
     1501 tcatggtgat gggtgtcatc tccctcacag gcgctctgcg agggaattga ttgggctgta
     1561 gagcaaactg tttgccaacg atcacgagag gagaaaactg caaggtttgc aatgggtttg
     1621 cagggctgaa tcactatggg gccaagtaca gatgtttcgg ctgagcgagc aagtcgtctg
     1681 tgcgatttct aaaaacattg tatatctgca gagatatgga ttctgacatt ttgtctcgat
     1741 gggataactt ttcctgcagg cgctccatga gggagtggtt tattgcgaac cggtgaccac
     1801 gcaggtcaca gtcgagcccc gtgaccgaga ctggcgtctt tctccccacg acagcaacgg
     1861 gtgatggctc accaccgcgg gcatcggtgc tgcttcaccc attcacgagc aggattttcc
     1921 tcgttgacct cgcgatcctt ccggctgtag cctaccccag gtacagctga tgtgtggacc
     1981 gcgccaatct attcgcacat aggtgttctc aatgagggcg atacgattac atcaagttgg
     2041 tgggcccgaa tcgcttcgcg atgaggaggc gcctaaaccc agtccgaatg acgatcaggt
     2101 acttgtccag gtctcggcca ccgcgattac accgacggag tttgcgtggt atcccacgtt
     2161 tcacaccctt gacggtggcg cacgaccgtt ccccttcatt ctcggtcacg aattctccgg
     2221 tgtggtcgcc gcgatcggac cggattccga cggagtacgg atcggagatt cggtgtatgg
     2281 attgaacgat tggttcggcg acggtgctca ggcggaatat tgtttgactg ttcccggcaa
     2341 ccttgcgccg aagcccactt cgctggtcga ttctctggca gcagcggtac cgatctcggc
     2401 ccttactgca tggcaggcac tcgtcgatcg ggctcagctc tcggaggggc agcgggtatt
     2461 gattcatggc ggagccggtg gggtcggcag ctttgccgtg cagttggccc gttacaagaa
     2521 ggctcatgtg atcgccacgg tgtcagcggt caatgccaat tttgtcacgg cgctcggtgc
     2581 cgacgaagtg attgattacc ggacgacgcc gtttgaggcg gtcgtccgtg acgtggatgt
     2641 ggtgctggat acggttggag gggagacgag ggaccgttcc tgggaggtgt tgaaaaaggg
     2701 cgggcgattg gtaactatcg cggctgatgc ggaacggttc agcgacccgc gagtgcgcga
     2761 tgccttcttc atcgtcgaac caaaccgggt tcagttgatc gaaatctcta gtctgatcga
     2821 tgccggcgtg atccgaccgg tcgtgggcgc cgtctttccc atggaggatg ttcagcacgc
     2881 ctacgagcag aaacctctgc gcggcaagca tgtgttgtat atcagcggaa cgtcatgagt
     2941 gttacgccat caccatgaag ggattctccc gccagtcaaa cagaaccggc caagcttctc
     3001 gtgtttcttt tctgatacag gctcattcgg tctgcggtgc acatgagtcg aagttctctg
     3061 tcacggagag aagattcgtt tgcaattccc tggatacact gcggcatcta atcttttaag
     3121 caccggtcca tttagtggta tttggcttgc ttcccctagt catgacctgc ccctctactt
     3181 ttttgcaggg agccgtggtt tctgctggat gcaccgaaat acataagtgt gaccgagagc
     3241 cacgaaactt tttttcaccg gaatcgaaag agatatggta tcagtagtaa atgtgaaaac
     3301 ggctatgatg gatctgcacg tctagaatca cagcggtaca acaccgaagc attcacgtat
     3361 aacttcggcg tacatattga cattacgggg aggcattcga tgagggcacg aattatcgca
     3421 ctgctctgct ttgctcttct tgtcagctgt gacacgcttc cctctcttaa acaatcggtt
     3481 gggatctcac aagatgtgcg cgatgaagcg cgtctggtga atcggtccgg catgtcgttc
     3541 ccggccgctg atgaaaatta ttttggcgac atggatgggg gtgccgcgct ctctcccgag
     3601 gaggtgaaag gtcgcaacac atggcttgtg tggagcggcg gcaacgaccg tttatggaat
     3661 gaactgtcaa cccgcagctt cgggacgctc gattttctga agacgctgtc ttcgcatccg
     3721 aatctgaaat ccacccgcga tactcgctgg tactatcttg gattggtcaa cgaaccgtgt
     3781 tttgaacagg ccaccggacc ggatccggat cggttcgggc tctggctcga caagcggatc
     3841 gtcggccctg gctgcccgcc agacccgttt gagaatgaag cgaaatatcc aggcgtaaag
     3901 atcggtgcac gcgggaagaa tctgccggtc ggatcgttct acggttacgc cagcggtatc
     3961 gtcgggatgc ggctgttccc caatccggac ttcgacgaag aggctgccaa gaaatgggat
     4021 gcgaaacggt attacgagga tccgacctac tatctctcaa aagatctcgt gaagccctat
     4081 cgtgtcggga tgtcctgcgc cttctgccac gtcgggccga acccggtgat gcctcccgag
     4141 aatccggagc atccggaatg gaaacatttg agctcgaacg tcggtgcgca gtatttttgg
     4201 atcgatcgga tcttttcctg ggaggcggat gagacgagct tcccatatca gctgttccat
     4261 acgtctcgcc ccggtgccct cgatacctcg ctggtttcga ccgactatat caacaacccc
     4321 cgtaccatga atgccgtata cacgctcggc cctcgtctcg aacaggccaa gcgctgggga
     4381 caagagaagt tgtccggtgg caacttgaac aataaacagc tcaacgacta tgtggcggaa
     4441 ggtccactga cgacattctt tcaaccgccg gacatcgtct tgactcctcg cgtgctgaag
     4501 gacggatccg attcggtcgg ggcgttgggg gcgctcaacc gcgtttttct caacatcggc
     4561 cttttcagcg aggaatggtt acgccacttc aatcccttgg tcggcggcaa accaattacg
     4621 ccgattcaaa tcatcgatgc aagaaacaac tcgagttact gggaggcgac agaggcccgg
     4681 actccaaatc ttgcactgtt cttcctgaag accaccgcgc cacacaaatt gaaagacgct
     4741 cctgaaggag accagttcct gacggaagat gtgtcactgc ttctgcaagg gaaggaggtc
     4801 tttgccgaac gttgcgcgcg ttgtcattcc agcaaacttc ctgtgccggc tgtcggcgtc
     4861 gacccgcacg gttgcgcagg ctctggttat ttggagtgct ggaagaagta ctggcagtgg
     4921 acgaagaccg aggaatttaa gcagaagatg cgggctattg ttcttgccga ggatttcctc
     4981 gaagggaact tcctgtccag cgatttgcgc atccctgtca cactgctcga gacgaatgct
     5041 tgtagcccac tggccaccaa tgctatccgc gggaacattt gggacaactt ctcgtcagaa
     5101 tcgtacaagg acttgccatc ggttggtgag atcaccgtgc accatccgat taccggggag
     5161 ccatggtcat acgtgatgcc tgcgggagga gtcggctata cgcgcgtacc gtcgctcatc
     5221 agcctctggt ccacagcacc gtttttgctg aacaacacgg ttggtagatt taatccgagc
     5281 ccgagcgtca agggacgcat ggagtctttc aataattcga tcgaacagct cctctggcct
     5341 gaaaagcgag acaaggacct gatcctgggc gataaggtgc cgggagtcat cgaccgaacg
     5401 acagcaagaa gctatttgcg tgtccccaat gggtatcttc cggacttcct tcagggcgtc
     5461 gtggaactcg aagcaaaggt tcttccggac gtgcagctgc ttccccccat tttcaaagaa
     5521 ggtgggatag aagtcggtcc cattcccaaa ggcactccgg tcaacctctt gacgaatttg
     5581 aacgtgctcc cggatgacgc aggctggatc gatcggctca tgcataagag gaaaattgca
     5641 aatctgttga tcaagatcaa aggtgcgctg aacgcgctgc cggaacacgc cagcgacgaa
     5701 gaagcccgga aggtctttca tgacaagggt ctggtgaagc ctctgctcga gttgagcaag
     5761 tgtccagatt ttgtcgtcaa ccgcggtcac tatttcggca cggatttctt caaagaggag
     5821 ccgggtctca ccgacgagaa gaagaaagcc ctaatcgcgt ttctgaagac attctagatc
     5881 gcgcctggtc agagaggagc ttcggatggc cgaaatctca agacgagtat ttctcaaact
     5941 ggccgctgca actgggatca ctctctcgct gggaggctgt tcgctcctcg ggccctcgcg
     6001 atcgataggc gagcggtgtg caggagccga tacggctcag cctgccgaat ctgaatatga
     6061 ttacatcgtc gtaggatcgg gcgccggagg cggacctgtg gcggctaact tggcgaatgc
     6121 cgggtatcga gtccttctcc ttgaagcagg gggcaaagag gagaatgcga actatgtcgt
     6181 tcccgccttt cacccgttgt ccacagaaga ccaggaccag caatggaact actttgtccg
     6241 gcattacgcc aacgacaatc gtcagaataa ggattcgaag cgcgacaaag ccggaagggg
     6301 gatctggtat ccgagggccg gtacgctcgg aggctgtacg gctcacaatg ccatgattac
     6361 ggtctgcccg cacgaaagcg attgggatcg catcgccgca ctcaccggag atgcctcttg
     6421 gtcgtctgag aagatgcgca gatacttcca gttggtcgaa cgttgccaat atagttcgag
     6481 gccattggat tttgtgacgg gaggccgtca tggatttcaa ggatggctat ccactgagcg
     6541 gcctactcaa aagacactgg aagcggtcgt caagaatgat ccgcagctcc ggcacattct
     6601 cactgccgtg gctctcacca cgttggaaga tctccccggg tccattccgg aggccattct
     6661 ccttcgagcc attccaaagc tgttcagcgc ggcattgagg gaagacatca atcacatgga
     6721 ttccatcaca cgccgccctg aaggtttgta cattacgcca ctcgcgacca atggcggcag
     6781 gcgcgccggc gtgcgtgatc gcattcgtga ggtgcaggag cgctgcccga atcagttggt
     6841 cgtcaaaacc aatgttctgg tcaccaaaat actgttggaa aagttaagga cccctcatgg
     6901 ggaacagtcg agagccattg gagtggagtg ccgtgaaggc gcgcatctct accgggcgga
     6961 cccgagggtc ggcgcagaca tgcctgcagt acaggaattt agggccaagc aggaggtcat
     7021 tctctcggcg ggagcgttca at
//
LOCUS       DATIPF010000431         3428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1053150, whole genome shotgun sequence.
ACCESSION   DATIPF010000431 DATIPF010000000
VERSION     DATIPF010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053150"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..969)
                     /locus_tag="VLL06_10755"
     CDS             complement(235..969)
                     /locus_tag="VLL06_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31487.1"
                     /translation="MAALAGDLTSVLNPFDLTLHFARQPEPDLEFTEEELDQTITTKT
                     RGTMPSPKKSGGGGRPILWIVLLALVGGLAYVATMEPEMLEEWLSPYLGESTPPPVAM
                     TPRPATPRPVAPPTPAPQVAPNPSNQTSAPEAQAPAPAAPVAPAVAPPPQTAARPVAP
                     VVSTLDPMFGEGQKVTITVDEMAPGGSIPLFADSSSSRPGPIVRAGASLTVMDGDLQP
                     GGWMYLVRTDEGVRGWVSEKRLRLKF"
     gene            complement(1092..2963)
                     /locus_tag="VLL06_10760"
     CDS             complement(1092..2963)
                     /locus_tag="VLL06_10760"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HSS31488.1"
                     /translation="MGVHSDELDTQDASRTITPAQSVNQLLSVYNSTMAVKKMTASSC
                     PPKRPRLRLSAHANVILRGRYLGRNVAGTIVETAAQLFWRVATDIARAEQLYPTAGRL
                     SHAALRWYECMARLDFLPNSPTLMNAGRRLQQLSACFVLPVEDSLASIYDGLKHQALI
                     HQSGGGTGFSFSHLRPHNDRVASSSGVASGPISFMRLFNLSTDVIKQGGTRRGANMGI
                     LRIDHPDILDFIALKQTPTEMTNFNLSVGITDSFMQALTHRRTYALINPLTNKPTGRF
                     PAQLIFDRLVEAAWTSGEPGLVFLDTINRANPTPRLGTIESTNPCGEQPLLAYESCTL
                     GSINVANFVTQPDARPTIDFARLAETISLAVRFLDNVIDRNHFPLLHIDRMTKQTRKI
                     GLGIMGFADLLIALNIPYNSEAAFRTATTLMDFFQSRAHLASTGLAAERGAFPAYRGS
                     RLQARHQRHRNATVTTIAPTGSISLIAGCSPGIEPLYGVHEVRRVMEGVLLTSVHPAF
                     VRRARALGLDLASLQLNLAAHPSIQHLTHIPEELRRLFVTAHDVSPEHHVRMQAVFQR
                     YSDSGVSKTINLPATATKAEVAAAFLLAYQLGCKGLTVYRTGSREEQVFSCSPSHPC"
     gene            2976..3389
                     /locus_tag="VLL06_10765"
     CDS             2976..3389
                     /locus_tag="VLL06_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HSS31489.1"
                     /translation="MKSYREELWFETKTRRAYLNITQQVETAVKRSGVREGLVLVNAM
                     HITASVYINDDEPGLLKDYDRFLDTLVPQSATYQHNETGEDNGDAHIKRQLMGREVVV
                     AITEGRLDFGPWEQIFYGEFDGLRRKRVLVKVIGS"
BASE COUNT          750 a          847 c         1114 g          717 t
ORIGIN      
        1 atgccgattt cagctagcgt ttgccgaaaa ccagcaaaat ggagtggctc cgtatccgcg
       61 atcacgccgt caaagtcaaa gatcaccgct gtcagtgtgg ccatgaaatt gactcgctac
      121 tgtgggtgtg gttacaggat atgaaacggg cggattataa cacagggcga gcgcctgaat
      181 acgcgagaat gtgtctactc gatcggcccc attcacggag gagagacgga agggtcagaa
      241 cttcaaacgg aggcgtttct cggataccca accccgtacg ccttcgtcgg tgcgcaccaa
      301 gtacatccag ccgccgggct gtaggtcacc gtccatgacg gtcagtgagg cccctgcgcg
      361 aacgatcgga ccaggcctgc tgctcgatga atctgcgaac aacggaatac ttccgccggg
      421 ggccatttcg tccaccgtga tggtcacttt ctgtccttca ccgaacatcg gatcgagtgt
      481 ggatacgacc ggggcaacag gtctcgctgc ggtttggggt ggtggggcaa ctgctggagc
      541 cacaggggcg gcgggagcag gtgcttgagc ctcgggggct gaggtctggt tactcggatt
      601 gggagcgact tgtggtgcag gtgtcggtgg cgccacaggc ctcggggtcg ccggcctcgg
      661 tgtcatagcc accggtggtg gggtgctctc accgaggtag gggctcagcc attcttccag
      721 catctccggt tccatggtgg cgacataggc caggccgccg acaagagcca ggagaacaat
      781 ccagaggatc ggtcgtcccc ccccaccgga cttcttgggg gaaggcatcg tgcctcgtgt
      841 ttttgtggtg atcgtttgat cgagttcctc ttccgtaaat tccaaatccg gttctggttg
      901 gcgagcgaaa tggagagtca aatcaaaagg gttcaagact gatgtcaggt ccccagcgag
      961 cgcagccatc gagcacctcc ctactgagcg attcaattcc acgtggtcta gcctgtgaaa
     1021 atacctatca ccatccccga gtggtgtcaa ttttgttgaa gaattatgcg tgccagcggc
     1081 gcgattcaga ttcaacaggg atgcgagggg ctgcaagaga agacttgttc ttcccggctg
     1141 cccgtccgat agacggtgag gcccttgcat ccgagttggt aggcgaggag aaaggctgcg
     1201 gccacctccg ctttcgttgc cgtagccggc aaattaatgg tttttgagac tccgctgtcg
     1261 ctatatcgct gaaacaccgc ttgcatacgc acatgatgct cgggtgagac atcgtgcgcc
     1321 gtgacaaaca gtcgtcgtag ttcttcgggg atgtgagtca gatgttgaat ggaagggtgc
     1381 gccgccaggt tgagttgaag tgaggcgaga tcgagaccga gcgcacgtgc ccgacggaca
     1441 aaggctggat gaaccgaggt gagcaggaca ccctccatga cgcggcggac ttcatgcaca
     1501 ccgtataacg gctcaatgcc aggcgagcaa ccggcgatga ggctgatgct gccggttggt
     1561 gcgatggtgg tgacggtcgc gttccgatgc cgttggtgac gtgcctggag tcggctgcct
     1621 cgataagcag gaaaggctcc gcgttcagcg gccaatcctg ttgatgcgag atgggcacgg
     1681 gattgaaaga agtccatcag cgtcgtcgcg gtgcggaacg cagcttcgga attatatggg
     1741 atgttgaggg caatcaataa atcggcgaag cccatgatgc ccaggccgat ctttctcgtc
     1801 tgttttgtca tgcggtcaat gtgcagaagc gggaagtgat tacggtcaat cacattgtcg
     1861 agaaagcgta cagcgaggga gatggtttcg gccaatcggg cgaagtcgat ggtgggtcgc
     1921 gcatccggtt gtgtgacgaa attggccacg ttgatggagc ccaaggtgca ggattcatag
     1981 gccagaagtg gttgttcgcc gcaggggttg gtcgattcga tggtgccaag gcgcggggtg
     2041 ggattggccc gattgatcgt gtcgagaaac accaatcctg gctcgccgct ggtccaagcc
     2101 gcttcgacga gccgatcgaa aatgagctgt gccgggaacc gtcctgtcgg tttgttggtc
     2161 aagggattga tgagggcata ggtacgacga tgtgtgaggg cttgcatgaa ggaatcggtg
     2221 atgccgaccg acagattaaa attcgtcatc tcggtcggcg tctgcttgag agcgatgaaa
     2281 tccaggatgt ccgggtggtc gatccggagg atgcccatat ttgcgccccg cctggttcca
     2341 ccttgtttaa taacatccgt ggaaaggtta aagaggcgca tgaaggagat gggcccagag
     2401 gcgacgccgc tggacgatgc gactcggtcg ttgtgaggac ggaggtgact aaacgagaat
     2461 cccgtgccgc cgcccgattg gtgaatgagc gcctgatgtt tcagtccgtc ataaatggag
     2521 gcgagagagt cttcgacagg gaggacgaag caggcggaga gttgctggag gcgacgtccg
     2581 gcattcatca gtgtgggaga attgggaaga aagtcgagac gggccatgca ctcgtaccag
     2641 cgtagggcag catgggacag ccggccggcg gtgggataga gttgttcagc tcgcgcgatg
     2701 tcggtcgcga cacgccagaa tagttgggcc gccgtctcga cgatcgttcc cgcaacgttc
     2761 ctgcccagat agcgaccacg gagaatgacg ttggcatgag ctgaaagacg aagccgcggg
     2821 cgcttcggcg gacatgagga cgcggtcatc tttttgacgg ccatcgtact attatatact
     2881 gacagaagct gattgactga ttgtgcggga gtaatggtgc ggcttgcatc ttgagtgtcc
     2941 agttcgtcag aatgaacacc cactgaccga cgatcatgaa atcctatcgt gaagagctct
     3001 ggttcgagac gaagacgcgg cgcgcctatc tcaacatcac acaacaggtt gagacggcgg
     3061 tcaagaggag cggcgtccga gaggggctcg tgctcgtcaa tgccatgcat attacggcca
     3121 gtgtctatat caatgacgac gaacctggct tactgaagga ctacgaccga ttcctggaca
     3181 cgctcgttcc tcagagtgcg acctatcagc ataatgagac gggagaggat aacggcgacg
     3241 cccacatcaa gcgacagctg atggggcgag aagtagtcgt cgcgatcacc gaaggtcggt
     3301 tggatttcgg tccatgggaa caaattttct atggggagtt cgacgggttg agacgaaaac
     3361 gcgtgttggt gaaggtgatc ggttcgtgac ccttcgggga taaactgttg gagcgcttgc
     3421 taccacaa
//
LOCUS       DATIPF010000432         8256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1233788, whole genome shotgun sequence.
ACCESSION   DATIPF010000432 DATIPF010000000
VERSION     DATIPF010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8256
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233788"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VLL06_10770"
     CDS             complement(<1..688)
                     /locus_tag="VLL06_10770"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HSS31490.1"
                     /translation="MGAHLHFDCYSGISGDMILGGLVDVGVPFPDLVNGLKRLRLSGF
                     TLRKRRVQRGAIHATKVDVVITTGLRKPLSLHRIRAILSASRIPEKIKQQSRSVFDRL
                     AEAEGQAHRMAKDHVHFHEVGVLDSFVDIVGGLICCDLLGVTRVTSSPVNVGAGTLQS
                     AHGILPVPGPAVAILAKGIPIYSAGPLRELTTPTGMALLRTLTSEFGSMPVVTPTAVG
                     YGAGDADPDGW"
     gene            977..1513
                     /locus_tag="VLL06_10775"
     CDS             977..1513
                     /locus_tag="VLL06_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31491.1"
                     /translation="MVTPCSLFFGLWSGRVVWMLLLCAAILLWPPLPASAKSKVHRAP
                     RPEPELKILELKVFPNPYRVSSGLLQFSALVKLPKELEGATLLEVSSFITSPSKNSLR
                     FLTTRTALEPHAASTDGTAPPQVSVFLTWDGLDQKKIPSGTGLYQYEVRAKLLANGEK
                     GPRTQMVAWPKRGTVEVR"
     gene            complement(1598..2350)
                     /gene="larB"
                     /locus_tag="VLL06_10780"
     CDS             complement(1598..2350)
                     /gene="larB"
                     /locus_tag="VLL06_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HSS31492.1"
                     /translation="MNPETLERLLTKVRTGTLSVSAAIGQLRTLPYEDLGFASVDHHR
                     SLRQGFPEVIFCEGKTSAQIRAIAKALLKHHRPLLATRATRQVATLIKRLDRRAAYHE
                     EARIVSIRDPQRQLQGHVLVITAGTSDVPVAEEAKVTAEVMGSRVETLYDVGVAGIHR
                     LLERQSRLMQARVVVVVAGMDGVLPSVVGGLVSCPVVAVPTSRGYGASFGGLAALLTM
                     LNSCAAGVGVMNIDNGFGAGCLAHRINVLKQA"
     gene            complement(2347..3366)
                     /locus_tag="VLL06_10785"
     CDS             complement(2347..3366)
                     /locus_tag="VLL06_10785"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HSS31493.1"
                     /translation="MSSIHRIGVIGAGAWGTALAKHLAEKGLETRLWAYEREVVDAIN
                     GKHENPVFLPGVALPPSLVATGSLTDLTEQSEGLLFAVPSHVARSVLRELVRSLPTSI
                     PFISATKGVEEDTLNLMTQVMGDVLPPAMQRNLMVLSGPSFAAEVSAGKPAVLCLAGS
                     NEELVKTFQAALMTPAFRVYADSDVLGVQLGGALKNVMALAAGVVDGLDLGHNVRAAL
                     ITRGLAEMVRLGVAMGADPRTFYGLSGVGDLVLTCTGALSRNHTVGVRLGKGERLETI
                     LSSMQAVAEGVRTAKAALGLARRHRVDMPIVQEINAVLFEDKSCRKAVTDLMERDAKP
                     EKGQA"
     gene            3649..4356
                     /locus_tag="VLL06_10790"
     CDS             3649..4356
                     /locus_tag="VLL06_10790"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSS31494.1"
                     /translation="MKTRQLAGRFAVVSLFTLLAPIVLYVPQAYAETYVAGQFGVTFP
                     QSLSDGKVTQDSLGGLDLSDQPLKNSLNFGGKLGHYFTRARWIGVETGVSYTTPHIKE
                     GSLTFTGPGGAATVGGLSGVHQRMIIWDVATLMIRYPGYRLQPYVGIGPALFFATLKG
                     PTAPPGQSAMGIGLNSEIGARYYITRNWALFGEGQYHYAPHLGYISNDSNPAADPFGF
                     RATYSAFTVSLGISYSF"
     gene            4425..4796
                     /locus_tag="VLL06_10795"
     CDS             4425..4796
                     /locus_tag="VLL06_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HSS31495.1"
                     /translation="MKLCPSCRHEQPEISRYCSQCGQRLHAEPVEDSPPPPPPRVELL
                     PGQLNIQFLYGMVAFLSLALLFPPWETPPSQPPEFLGMHFILDPPTPDAIVSRLLLTI
                     ELVTIAIAGMYGAFLFRQKKP"
     gene            complement(5160..5960)
                     /locus_tag="VLL06_10800"
     CDS             complement(5160..5960)
                     /locus_tag="VLL06_10800"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HSS31496.1"
                     /translation="MSRLLVCPPDYFAIEYEINPWMRRSNAVNSDQAVRQWHHLMQVL
                     EQDVGAVLDRMKPVPGLPDLVFTANAGVVVGNKAVPSRFRYPERQREEAYFEDWFRGQ
                     GYEVVTLDSGHFFEGAGDLLGFPDTWFGGYRQRSDIHVFSTLSDWFHHEAIPLELVDN
                     RFYHLDTCLCPLSGGELLYFPPAFDHYGQEALAERIAPPRRLCVPEAEALRFACNAVC
                     VGKHVVLPTGCPETESLLTSRGYLPHPVPLDEFMKSGGSAKCLTLALD"
     gene            complement(5957..7201)
                     /locus_tag="VLL06_10805"
     CDS             complement(5957..7201)
                     /locus_tag="VLL06_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HSS31497.1"
                     /translation="MMQDQETVLLQGHIIDSLILAKVLDTILMMGGTFDLQDVQIGKT
                     REEPSRARIIVRASSAKLLNEIVHAIQPHGASVESERDCRLAAAPADGIFPNEFYATT
                     HLPTQVRMHDQWLEVEGIEMDLGIRVDPVSLTARTIPMGHVTRGDLIVIGREGVRVIP
                     LQRPRDRDVFSFMASQVSSERPHGHIIGDIAKRLAQLRAGFREAKAGSKVLLAGGPAI
                     VHAGGREALTWLIDEGFIHVLFCGNALPAHDMEVDLYGTSLGYGHAAGRVVPHGHEHH
                     LRTINRIRAIGSIEAAVTSGVIKTGIMAACVRRGVQVVMAGTIRDDGPLPGVITDAIE
                     AQDAMRAAIPGIGLALLVASTLHAVATGNLLPATIPSVCVDVNPAVPTKLADRGTFQA
                     VGLVMDAASFLRDLARELGWKP"
     gene            complement(7409..>8256)
                     /gene="truD"
                     /locus_tag="VLL06_10810"
     CDS             complement(7409..>8256)
                     /gene="truD"
                     /locus_tag="VLL06_10810"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HSS31498.1"
                     /translation="AIGVAGLKDARAVTTQMVSLLGVTPDRLDRLNVDEQVLSVEVLG
                     RHRNRLRPGHHAGNHFRLVIREVATHARETVPLATDMLVRRGVPNYFGPQRQGRSGQN
                     YQIGAELLVDPARRNRLSRSKRMWYLNAYQSHFFNDILARRLDRLDRILVGDWAMKME
                     NGACFPVEDATKEQPRADRFEISPTGILFGSRVSWASGEPGEIERAVVAESGATPESL
                     TEGAKACGFRGERRSLRIPLTGLEWDVDGTVLTLSFSLPPGAYATSVLRELMKTDSQA
                     VENVR"
BASE COUNT         1811 a         2606 c         2190 g         1649 t
ORIGIN      
        1 gccacccgtc gggatcagca tcgcccgccc catagccgac ggcggtcggt gtcaccaccg
       61 gcatggagcc gaactccgac gtcaacgtgc gcaacagcgc catgccggtc ggcgtggtga
      121 gctcacggag agggccggcg ctgtatatcg gaatcccttt cgccagaatg gcaactgccg
      181 ggccaggcac cggcaggatc ccgtgagccg attgcagcgt tcccgctcca acgttgacgg
      241 gagaggatgt cacacgcgtc acaccgagca gatcgcaaca gatcagcccg ccgacgatat
      301 caacgaagga atccaggact cccacttcat ggaaatggac gtgatccttg gccatccgat
      361 gagcctgccc ttcggcttcg gctaatcgat cgaacaccga tcgactttgc tgtttgatct
      421 tttcggggat tcgactcgcg gagaggatcg cgcgaatccg atgaagcgag agcggcttgc
      481 gcaatcctgt cgtgatcacg acgtccactt tcgtggcatg gatcgcccct cgctgcaccc
      541 gccgctttcg cagcgtgaac cctgaaagtc tgagacgttt cagcccgttc actaagtcgg
      601 gaaacggaac tcccacatcc acgagtccgc cgaggatcat gtccccgctg atgccggaat
      661 aacaatcgaa atggagatgc gcgcccatgt gcgtcctttc tctgctcaaa ccgcgcccat
      721 cttacccaag gcacaggcga acggcaatcg gcgagaggtc tatccggcat ggtgttcgca
      781 aagaggcgaa ggaggtgcgg cctgatgcaa aggccgagaa cgacgcagat ggttgcgcat
      841 cgttcgccgt agcagaacga tcaatgttgg acaggcacct cgcacaagca ttgacagcct
      901 accgacgctg tgttatcccc actgcactcg ccaaccaact gggcgcttcc taacaactat
      961 gacgatttgc cgatccatgg tcacgccctg ctcactattc ttcgggttgt ggagcggtcg
     1021 ggtcgtctgg atgctcctgc tttgcgccgc gatactgctc tggcccccgc ttcccgcctc
     1081 ggcaaaatcg aaagtccacc gagccccgcg ccccgaaccg gaactcaaga ttctcgagct
     1141 caaagtattc cccaacccct atagggtctc gtccggcttg ttgcagtttt ctgccttggt
     1201 taagctgccc aaagaactgg agggggcaac cctgctggaa gtctcctcat ttattacatc
     1261 gccgtcaaaa aactcgcttc gttttctgac cacgcgtacc gcgctggaac ctcacgccgc
     1321 ctcgaccgac ggcaccgccc ctccacaagt ctcggtcttc ctgacatggg acggattgga
     1381 ccagaaaaaa atcccgtcag gtacaggcct atatcaatac gaagtccgtg ccaagttgct
     1441 ggccaacggc gagaaaggtc ccaggacaca gatggttgcc tggccgaaac ggggcaccgt
     1501 ggaagtccga taatagacgt gaaaagtgaa acgttaaacg tgagaggtgt gcataaagaa
     1561 ctcacctcgt atcacttcat tttttacgtt ccatatttca cgcctgcttc agtacattaa
     1621 ttcggtgggc aaggcagccg gctccgaacc cattgtcgat gttcatcact ccgaccccgg
     1681 ccgcacagga gttcagcatg gtcaataacg cagcgagccc gccgaaactc gcgccatagc
     1741 cccgactcgt cggtacggcc acgaccggac aggaaaccag tccgccgacc acacttggca
     1801 agactccgtc cattcctgca accaccacaa ccacccgggc ttgcatcagc cggctctggc
     1861 gctccagcaa ccgatgaatg ccggccacgc ccacgtcata gagcgtttca acccgactcc
     1921 ccatcacctc tgcggtcact ttcgcctctt ccgcgacggg aacgtccgag gtgcccgctg
     1981 taatcaccaa gacatgtccc tggagttgcc gctgggggtc acggatcgac acaatgcgcg
     2041 cctcctcatg ataggccgct cggcgatcga gccgtttgat aagcgtggcc acctggcgcg
     2101 tcgcacgtgt cgccaacaag gggcgatgat gtttcaacaa ggccttcgcg atggcacgaa
     2161 tctgggctga cgtcttcccc tcgcagaaaa tgacctcggg aaacccctgc cgtaacgacc
     2221 ggtggtgatc gactgaggca aatccgagat cttcataggg caacgtgcga agctgtccaa
     2281 tcgccgcgga gacggacagt gtacccgttc gcaccttggt cagtaatcgt tcaagtgtct
     2341 caggattcat gcctgccctt tctccggctt tgcgtctcgc tccatcagat cagtcaccgc
     2401 cttacggcag gatttgtcct cgaacaacac tgcgttgatc tcttgcacga tcggcatgtc
     2461 gacacgatga cggcgcgcga gtcctaacgc ggccttcgcc gtccgtacgc cttcagctac
     2521 ggcctgcatg ctgctgagga tcgtctccag ccgctcaccc ttccccagcc ggacaccgac
     2581 cgtatggttc cggctcaggg cccccgtgca ggtcaacacc aagtcgccga cgccggacag
     2641 cccataaaac gttcgtggat ccgcacccat cgcgactccc aaacgcacca tctccgccaa
     2701 ccctctcgta atcaacgcag cccgcacatt gtgaccgaga tccaaaccgt ccaccacgcc
     2761 ggccgccagc gccatcacgt tcttgagcgc gccgccgagc tggacgccca agacatcgct
     2821 gtcggcgtat acccgaaaag ccggcgtcat caatgcagcc tggaaagttt tgaccagttc
     2881 ctcgttgctc ccggctaaac agagcaccgc cggtttccct gcgctgactt ctgccgcgaa
     2941 gctcggaccg gacaagacca tgaggttccg ttgcatggca ggaggaagga cgtcgcccat
     3001 gacctgagtc atcaaattca acgtatcttc ttcaactccc tttgtcgcac tgatgaaggg
     3061 aatagaagtg ggaagggacc ggaccagctc gcgcagcaca gaacgtgcga cgtgggacgg
     3121 caccgcgaag agcagcccct cgctctgctc ggtgagatcg gtcaacgatc cggtggcaac
     3181 gagcgatggc ggaagggcga caccgggcag aaagaccgga ttctcgtgct tcccgttgat
     3241 cgcatccaca acctctcgct cataggccca gagccgagtt tcgaggcctt tctcagcaag
     3301 atgcttcgcc agggctgtgc cccacgcgcc agccccgatc actcctatgc gatggatgga
     3361 tgacatgatt tggatagtga taatgacgga aggagtgaca tcaatcgggt ttgcgaacta
     3421 cgcggattat aggaaccggt tctttcacct gtcaatgaat gagaggcagt ctttgaaatt
     3481 tgtcaagtca ggcagggctc catgggactg gcaatacagc gtcggaagaa cagagtggca
     3541 tatgccttgc tccttccccg taagatttca caattttctt cgtccagacg aatcgggcga
     3601 ctagtatgaa agcgggcatg gtttcaggtg atagtgaggg agatggccat gaaaactaga
     3661 caattggcag ggcgtttcgc ggtcgtgagt ctcttcacgc tcctggcgcc gattgttctc
     3721 tatgtgcctc aggcgtatgc agaaacctac gtggcggggc agttcggcgt gaccttcccg
     3781 cagagcctaa gcgatggcaa ggtcacgcag gattccttag gggggctgga cctttccgac
     3841 cagccattga agaactccct caattttggc ggaaaactcg gtcactactt cacaagagca
     3901 cgctggattg gtgtcgagac cggcgtgtcc tacaccaccc ctcatatcaa agaaggatcg
     3961 ctcacattta ctggccccgg tggagcagcc actgtcggag ggttgtcagg tgttcaccag
     4021 cgcatgatta tttgggatgt ggccactctg atgattcgct acccaggcta tcgtctccaa
     4081 ccgtatgtcg gcatcggccc cgccctcttc ttcgcaacac taaaaggacc aacagctcct
     4141 ccaggacagt cggccatggg tattggccta aatagtgaaa ttggtgcccg ctattacatt
     4201 acccgtaact gggctctgtt tggagaagga caatatcact atgctcccca cctaggctac
     4261 atttcgaatg atagcaaccc ggccgctgac ccatttgggt ttcgcgcgac ctacagtgcg
     4321 ttcacggtga gcctcggcat cagctacagc ttctgacact tcgcctccca ctccccatct
     4381 agcaaaccaa cgcagttcca tgtagcctac gcacggaact gcccatgaaa ctttgtccat
     4441 catgccgaca tgaacaacca gagattagcc gctactgctc gcagtgcggc caacggctcc
     4501 atgcagagcc cgttgaagac tccccaccac cgccaccacc acgagtcgaa ctactgccag
     4561 gtcagttgaa tatccaattt ctttacggca tggtggcgtt cttgtccctc gcgctcctct
     4621 ttccaccctg ggaaacccca ccgtcacagc ctcctgaatt cctcggcatg catttcattc
     4681 tggacccacc gacgcccgat gccatcgtga gccgattgct gctgacgatc gaactcgtca
     4741 ccatcgccat tgcaggaatg tatggggcgt ttctcttccg gcaaaaaaaa ccctagcagg
     4801 atgctgaaaa agctcgccag ctgcgttctc gtatcgttca gaccctcaac ggggactcgg
     4861 ccgcctcacc actcggcggc gcgcacagac gtggtgctcc ttactcgtcg caccgtgcgc
     4921 cccaagggta cgcctcggcc cttcactcac tgcggccttg ctggacgaac tttttgagca
     4981 tcctgaacga tgtcctcccg ctctcccaga catatagccc agtgaaactc cggtgtgtct
     5041 gccgaattct tccgaggtct gctagcccgt caatagtcat ccgtcatcgg caggtgaaaa
     5101 aagggcgggt ggtccgcagt tttgaccaga gtctatttac cactgaccag cgacacggct
     5161 cagtctaacg ctagggtgag acacttggcc gagccgcccg acttcatgaa ttcatcgagc
     5221 ggaacaggat gaggaagata gccgcgcgag gtcagcaggg attctgtttc cggacagcct
     5281 gtcggcagca ccacatgttt cccgacacag acggcattgc aggcaaaccg aagagcctca
     5341 gcctccggca cgcaaagacg tctgggagga gcgatccgtt cggcaagagc ttcttgccca
     5401 tagtgatcaa aggcgggagg gaaatacagc agttcgccac cgctcaaggg gcacaagcag
     5461 gtatcgaggt ggtagaaccg gttgtcgacc agttcgagtg ggatggcctc atggtgaaac
     5521 cagtcgctga gtgtcgaaaa cacatggata tctgatcgct gtcgatagcc accgaaccag
     5581 gtatcgggga atcccaagag gtctccggca ccctcaaaga agtgcccaga atcaagcgtc
     5641 acaacttcat acccctgccc acgaaaccag tcctcaaaat atgcctcttc ccgctgccgc
     5701 tccggatagc gaaaccgact cggcacagcc ttattcccaa cgacgacgcc ggcatttgcc
     5761 gtgaacacca agtcaggcag tcccggcacc ggcttcatcc gatccaggac cgcacccaca
     5821 tcctgctcaa gcacctgcat gaggtgatgc cactggcgga cagcctggtc tgaattgacg
     5881 gcgttcgaac ggcgcatcca tggattaatc tcatattcga tcgcgaaata atctggcggg
     5941 cagaccagta atcgactcat ggcttccatc ccaattcacg agccaggtcg cgcaaaaacg
     6001 acgcggcgtc catgaccagc ccgacagctt gaaatgttcc tcgatcggcg agttttgtcg
     6061 gaacagccgg attgacgtcg acacagacgg atggaattgt cgcaggcagc aaattgcccg
     6121 tcgccaccgc gtgcagcgtc gaggcgacga gtaaggccaa gccgataccg ggaattgccg
     6181 cacgcatggc gtcttgagcc tctatcgcat ccgtaatcac accgggcaac ggaccgtcat
     6241 cgcgaatcgt gcccgccatg acgacttgaa caccccgcct gacgcaggcc gccataattc
     6301 cggtcttgat gaccccggac gtgaccgctg cctcgatgct gccgatggcc ctgatcctat
     6361 taatggttcg aagatggtgt tcatgcccat gcggcacgac gcgtcctgct gcatgcccat
     6421 agccgaggga cgtcccatag agatccactt ccatatcatg agcgggcagc gcgttcccgc
     6481 agaacaagac atggatgaat ccttcatcga tcagccatgt cagcgcctcg cgccctccgg
     6541 catgcacaat cgctgggcct ccagccaaga gcaccttact gcccgctttc gcctctcgga
     6601 accctgctcg caactgcgca agacgcttgg cgatgtcgcc gatgatatgc ccgtggggac
     6661 gttccgacga gacctgtgat gccatgaaac tgaatacatc gcggtcgcgt gggcgttgca
     6721 gcgggatcac gcgcacaccc tcacggccga ttacaatcag gtcgcctcgc gtcacatgcc
     6781 ccattgggat ggtacgggca gtcaggctca ccggatcaac gcggattccc aaatccatct
     6841 cgatcccttc cacctccagc cattgatcat gcattcgcac ctgggtcggc aaatgggtgg
     6901 tcgcgtagaa ctcattggga aagatgccgt ccgccggcgc cgcagccagc cggcaatccc
     6961 gttcactttc gactgacgcg ccatgaggtt ggatcgcatg gacaatctca ttgagcaact
     7021 tggcggacga ggcccgtaca atgattctgg cgcgcgacgg ctcctcacgc gtcttgccga
     7081 tctgcacgtc ttgaagatcg aatgtgccgc ccatcatcaa aatcgtgtcc agcaccttcg
     7141 ccagtatcag ggaatcgatg atatgtcctt ggaggaggac ggtctcttga tcttgcatca
     7201 taaaatcaga ctccctactc ttatggatag cctccgcaca gattctacca ccagtatcaa
     7261 cccgggtcgg gaatgttgag gatttcttcg acccgagaac gaacctatgc aggcggttca
     7321 aaatgttcgc tagcgaggcc gcaggcgagt gaaaaccgga ggcgtaccct ccgggtacgt
     7381 cgaggttttt cacgaaccga gaacgaagct agcggacatt ttcaaccgcc tgcgagtcgg
     7441 ttttcatgag ttcgcgcaag acactggtcg cataagcacc ggggggaagt gaaaaggaaa
     7501 gtgtcagcac tgtgccatcc acatcccact ccaagccagt aagagggatt cggagcgaac
     7561 gccgctcccc acgaaacccg caggccttcg cgccctcagt gagactctcg ggcgtggccc
     7621 cgctctcagc aaccacggcc cgttcgatct cgcctggttc cccgctggcc cacgaaacac
     7681 gtgagccgaa caagatgccg gtcggactga tctcgaaccg gtcagctcgt ggttgttcct
     7741 tcgtcgcatc ctcgaccgga aaacaggcgc cattctccat tttcatagcc cagtctccaa
     7801 ccaaaatgcg atcgagtctg tccagccgtc ttgccagaat gtcattgaag aagtgcgatt
     7861 gataggcgtt gaggtaccac atgcgcttcg accggctcaa tctgtttcga cgagcaggat
     7921 ccaccagtag ttcggcaccg atctggtagt tctgtccgct ccgcccttgc cgttgcggac
     7981 cgaaatagtt gggcacgcct cgccgcacca gcatgtccgt cgccagaggc actgtctcgc
     8041 gggcatgcgt cgccacctct cgaatcacca atcgaaagtg attcccggca tgatgcccag
     8101 ggcgtaaacg attgcgatgc cgccccagca cctccacgga caagacctgt tcgtccacgt
     8161 tcagtcgatc cagccggtca ggcgtgacgc cgagaagcga gaccatctgc gtggtgacgg
     8221 cccgggcatc tttgagaccc gccacaccga tcgctt
//
LOCUS       DATIPF010000433         2556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1409910, whole genome shotgun sequence.
ACCESSION   DATIPF010000433 DATIPF010000000
VERSION     DATIPF010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409910"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VLL06_10815"
     CDS             complement(<1..1175)
                     /locus_tag="VLL06_10815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS31499.1"
                     /translation="MKTPLRLLQLEDNTVDAELITATLAEGGIPCQSQLVDTRQAFVA
                     ALKEGRMDLILADYSIPGFDGMTALTLARQHCPDVPFLFVSATIGEELAIDAMHQGAT
                     DYVLKQRLGRLVPSVQRALRELDDRTERKRAEEALRQSEKQFRQSQKMEAVGRLAGGI
                     AHDFNNLLTVIMGYSQVLLTELGPQHPLRGKIDETLKAGERAATLIRQLLTFSSKQSL
                     DPKILSLNTAVTSLESLLRRLIGEDIQLVSTLDPANGRLRADQAQLEQVLVNLVVNAR
                     DAMPKGGTLTIETAQVELTRSPVYHLTPLPPGLYVRLAVSDTGCGMDRNTQSHIFEPF
                     FTTKGEGKGSGLGLSTVFAIVTQCGGAIDVTSRVGHGTRFDLYFPSVESDILTTAPT"
     gene            complement(1194..1649)
                     /locus_tag="VLL06_10820"
     CDS             complement(1194..1649)
                     /locus_tag="VLL06_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS31500.1"
                     /translation="MTTVKPILLAEDNPRDAELALAAMEEHHIADKVVVCHDGAEVLD
                     YLYCRGIFATRQQGNPVVVFLDLKMPKIDGLEVLKTIKNDVNLKPIPVVMLTSSREER
                     DLAQSYALGANAYVVKPVEFHQFITAVKELGVFWGVINEPPPESSEPIE"
     gene            complement(1646..>2556)
                     /locus_tag="VLL06_10825"
     CDS             complement(1646..>2556)
                     /locus_tag="VLL06_10825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS31501.1"
                     /translation="RLMTELDSAERRTLLLRVTQSAGSTRNTIALLGLGALMQLVLLA
                     SVYFLIHHDVTQRRRVAAELQSRGELLQAANKELEAFSYSVSHDLRAPLRHIDGYAAL
                     LSKSAGEALNDKARRYLQTISDSAKQMGQLIDDLLVFSRMGRQDMLRTTVNLDQLVKT
                     VLHDLRLDLQGRTISWTMHPLPNVLGDPAMLRQVFVNLISNAIKFTATRPEAKIEIGA
                     VTRKSGEAEVFVRDNGVGFDMQYVNKLFGVFQRLHRNDEFEGTGIGLANVRRIIHRHG
                     GQTWAKGAPDQGATFYFSLPLAGSLR"
BASE COUNT          536 a          706 c          747 g          567 t
ORIGIN      
        1 tgggtcggcg ctgtggtgag gatgtcggac tcgacgctcg ggaaatacag atcgaagcgt
       61 gtgccgtggc ccactcggct ggtgacgtcg atggcaccgc cgcattgggt gacaatcgca
      121 aataccgtag agaggcccag accgctgccc tttccttccc ccttcgtggt gaagaatggc
      181 tcgaagatat gggattgtgt gttgcgatcc atgccacagc cggtatcgct gacggctaac
      241 cggacataga gacctggcgg caacggggtg aggtggtaga ccggactgcg ggtcagttcg
      301 acctgggccg tttcaatcgt cagtgttccg cccttcggca tggcatcccg cgcattgacg
      361 acaagattga ccaggacctg ttcaagctgg gcttggtcgg cgcgcagccg cccattcgcc
      421 ggatcgagtg tactgacaag ctggatgtcc tcgccgataa ggcggcgcag cagactttcc
      481 agactcgtga cggcggtatt gagtgacaga attttcggat ccagcgattg tttgctgctg
      541 aatgtgagca gttgccggat caacgtcgca gcccgttcgc cagccttcag tgtttcgtcg
      601 atctttccgc gaagggggtg ttgcgggcca agttctgtca agagaacttg gctgtagccc
      661 ataatgacgg tcaggagatt gttgaagtca tgcgcaatcc ctccagccaa ccgaccgact
      721 gcttccatct tctgggactg ccgaaactgt ttctcgcttt gtcgaagggc ctcttccgcc
      781 cgtttccgtt cggtacggtc atcgagttca cgtagtgcac gctgaaccga aggcaccagc
      841 cgcccaagcc gttgcttgag cacatagtcc gtcgcacctt gatgcatggc gtcgatagcc
      901 agttcttctc cgatggtggc ggagacaaag agaaagggca cgtcgggaca gtgctgacgt
      961 gccaaggtca gcgccgtcat gccgtcgaag cctgggatgg aatagtccgc caggatcagg
     1021 tccatccggc cctccttgag ggccgcgaca aatgcctgac gtgtgtcgac gagttgtgac
     1081 tggcagggga ttccaccctc ggccaaggtg gccgtgatca gttcggcatc aaccgtattg
     1141 tcctcaagct ggagtaggcg gagcggggtt ttcacaattc ctttatgagg aaactactcg
     1201 atcggctcag agctttcggg gggcggttcg ttaatcacac cccaaaagac accgagttcc
     1261 ttcacggccg tgatgaactg gtgaaactca actggcttca cgacgtaggc gttggcaccc
     1321 aacgcataac tttgggccaa atcgcgctcc tctcgcgagg acgtgagcat gaccacggga
     1381 atcggtttca ggttcacgtc attcttaatg gttttcaaga cttcgagacc gtcgatcttc
     1441 ggcatcttga gatccaagaa cacgaccaca ggattgccct gctgcctggt ggcaaagatt
     1501 cctcgacaat agagatagtc gaggacttca gcgccatcgt gacagaccac cactttgtcg
     1561 gcgatatgat gttcctccat cgcggccaac gcaagctcgg catcacgcgg gttatcttca
     1621 gcgagcagga ttggcttgac tgtggtcatc ggagagaccc cgcgaggggg agcgaaaagt
     1681 agaaggtggc cccttggtcc ggtgcccctt tggcccaggt ctgtcctcca tgccgatgga
     1741 tgattcgacg gacgtttgcg agaccgatac cggtcccttc aaactcgtcg ttccgatgaa
     1801 gacgctgaaa cacgccgaac aacttgttga catactgcat atcaaatccg accccattat
     1861 cacgcacgaa cacctcggcc tcacccgatt ttcgcgtaac ggcgccgatc tcgatcttgg
     1921 cctctggcct cgtggctgtg aattttatcg cattggagat caaatttacg aagacttgac
     1981 gcaacatcgc cggatctccc aacacgttag gtagtgggtg cattgtccag gagattgtcc
     2041 gcccttgcaa gtcaagacgt aaatcgtgaa ggacggtctt gacgagttga tccaaattga
     2101 ccgttgtacg gagcatgtcc tggcgaccca tacgggaaaa gacgagcagg tcatcgatga
     2161 gttgtcccat ctgcttcgcg gagtctgaaa tcgtctgcaa atagcggcgc gctttgtcgt
     2221 tgagcgcctc accggcggat ttggacaaca acgccgcata cccgtcgata tgacggaggg
     2281 gagcgcgcag gtcatgcgac acggagtagc tgaacgcttc caattcctta ttggcggcct
     2341 ggagcagttc tcctcggctc tgcaactccg cggccacgcg tcgtcgctgg gtgacatcgt
     2401 gatggatcag aaagtagacg gaggccaaga ggaccaattg catgagtgca ccgagtccga
     2461 gcaaggcgat tgtatttctc gtgcttccag cggattgcgt cactcgcaat aagagggttc
     2521 gtcgttcggc cgagtcgagc tccgtcatca gtcgat
//
LOCUS       DATIPF010000434         4203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_967548, whole genome shotgun sequence.
ACCESSION   DATIPF010000434 DATIPF010000000
VERSION     DATIPF010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967548"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VLL06_10830"
     CDS             complement(<1..627)
                     /locus_tag="VLL06_10830"
                     /inference="COORDINATES: protein motif:HMM:NF013857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HSS31502.1"
                     /translation="MVKSPVADIRCVVRLALQEDLPLGDVTTAALFPSPAPALARIVA
                     QQSLVVAGLAAAVQTFRTVDASLVLSIQRQDGDPVQDGDCLLQIEGDGRSILKAERVA
                     LNFLQHLSGVATLTRQFCHAVRGYPVTILDTRKTIPGLRALQKWAVLLGGGTNHRQSL
                     SEGILIKDNHLALLNHTTASVKTACYKAKTTAPHTATVIVEVESLADVQ"
     gene            complement(631..3414)
                     /locus_tag="VLL06_10835"
     CDS             complement(631..3414)
                     /locus_tag="VLL06_10835"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HSS31503.1"
                     /translation="MSTPQLDKTYDSKAVEARWSQVWNQKGYFHASPTHSGQPYCIVI
                     PPPNVTGSLHVGHALNHSLQDILIRWRRMQGLNVLWLPGTDHAGIATQNVVEKQLLAE
                     GISRESLGRERFIERIWQWKATSGDTIIRQQKRLGESCDWERLRFTMDEGLSKAVLEV
                     FVRLHEDGLIYRGERLINWCPRCLTALSDIEVEHEEIKGKLYSIDYPLEQDPTIRLTI
                     ATTRPETMFGDSAVAVHPEDPRYNRLIGQRVRLPLTHRTIPIVGDAILVDREFGTGVV
                     KITPAHDFNDFEAGERHGLPRYSIFDHQALLDPEGLTTAGVEQAVLHAVATLPVPKAR
                     PKIEQALKDRGLLTKVDDHKMAIGKCYRCKTIVEPYLSPQWFVKIKPLAEPAIQAVED
                     GRIRIIPEGWTNNYLGWMREIKDWCISRQIWWGHQIPAWYCRSCNKESLVETEHRVAG
                     ITYGPEHIPKRLVILSTAQPIVAKHAPASCHVCGGTEFIRDPDVLDTWFSSALWPFST
                     LGWPEHTPELKTYYPTSTLVTGLDILFFWVARMIMMGLKFMDDVPFREVYIHALVRDA
                     EGQKMSKSKGNVIDPLHVMEQFGTDALRFTLASMASPGRDIKLAEERIEGYRNFANKI
                     WNATRFSLMNLDGPRTAVAPTERAFPDRWILSRLNQTIRSVTAELEAYRFDRAASALY
                     QFIWHEYCDWYLELIKPTLQNTASPEGPGTRQTLVETLETTMRLLHPFMPFLTEEIWH
                     TLPHQGESIVIQRYPIFDTTWAAPEMDQRFTLLEQTIGLVRTSRVLLNYPPGQQITFY
                     VAHDEAQQQSHLDQLRSYLTHLGRGTVILTPTAHWPTNNLLRLVTEGLSVGIAVAGDV
                     DLNKALDRIIKEQSEQTKEIERLEGKLRNQEFTAKAPPEVITDHQDRLTSLRRDQAML
                     TGSGQQLRAMLGT"
     gene            complement(3784..>4203)
                     /locus_tag="VLL06_10840"
     CDS             complement(3784..>4203)
                     /locus_tag="VLL06_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31504.1"
                     /translation="QLGRSLSEERLTQAIEPVLMTALPKILSQEISLLKPVIRTSVSE
                     IASPLIKERIDQFVREELNAVRTALSDMVQGELKSIEGLVKEEIQQAAAKQTSQTVER
                     LVRTTAKEQVEQTVQRLVPDLAEAQIKAEITRLTQAA"
BASE COUNT          835 a         1086 c         1297 g          985 t
ORIGIN      
        1 ttgcacatct gccaacgatt ccacttccac gatgaccgtt gccgtatgag gagcagtggt
       61 cttcgccttg tagcaggcag ttttgactga cgctgtcgtg tggttcaaga gggccagatg
      121 attgtccttg atgaggatgc cctcgctcag cgactggcga tgattcgttc ctccgccgag
      181 gaggactgcc cacttttgaa gtgcgcgaag gcccgggatc gtttttcttg tgtccaggat
      241 cgtcacgggg tatccgcgaa ctgcatggca gaactgacgc gtcaaggttg cgacgccgga
      301 caggtgttgg agaaagttca gggccactcg ttcggccttc aggatggagc gcccatcgcc
      361 ttcgatctgg agaagacagt ctccatcctg cacagggtcc ccgtcctgac gctggatgga
      421 caagacaagg gaggcatcga ctgttcgaaa ggtctgcact gcagcggcga gaccggcgac
      481 gaccagcgat tgttgagcga cgatgcgtgc cagcgctggt gccggagagg gaaagagtgc
      541 ggctgtcgtg acgtcgccaa gagggaggtc ttcttggagg gcgagtcgaa cgacacaccg
      601 tatgtcggca accggggact tgaccatcgg ttacgttccc agcatggccc gcaattgttg
      661 tccactgccg gtgagcatgg cctgatcccg gcgcaggctt gtgagacgat cttggtggtc
      721 ggtaatgact tccggcgggg ccttggccgt aaactcctgg ttgcgcagtt ttccttccag
      781 ccgctcaatt tcctttgtct gctcagattg ctctttgatg atgcgatcca gtgctttgtt
      841 gagatcgacg tcgcctgcca ctgcaatgcc gacggataga ccctctgtca ccaatcggag
      901 aaggttgttg gttggccaat gagccgttgg ggtgagaatg accgtaccgc ggccgagatg
      961 ggtgaggtag cttcgaagct ggtccaagtg actctgttgc tgggcctcgt catgcgcgac
     1021 gtagaaggtg atctgttgcc ccggtgggta gttcaagaga accctcgatg tgcggaccag
     1081 accaatggtc tgttcgagca gtgtaaagcg ttggtccatc tcgggtgcag cccatgttgt
     1141 atcgaagatg ggatagcgct gaatcacgat gctctcaccc tggtgtggga gcgtgtgcca
     1201 gatctcttcc gtgagaaacg gcatgaacgg atgcaggagg cgcatcgtgg tttcgagcgt
     1261 ctcgacgagg gtctgtctgg tccctggtcc ttccggactc gccgtgtttt gaagtgtggg
     1321 cttgatcagt tcgaggtacc agtcacagta ctcatgccag atgaattggt acagcgcgct
     1381 cgcggcgcga tcgaaccgat aggcttccaa ctcggccgtc accgaccgga tcgtctgatt
     1441 caggcgactc aaaatccagc ggtcgggaaa ggcacgctcg gttggtgcga cggccgttcg
     1501 tggcccatcg agattcatga ggctgaagcg cgtcgcgttc cagatcttgt ttgcgaaatt
     1561 gcgatagcct tcgatccgtt cctccgcaag tttgatgtca cgccctggtg aggccatcga
     1621 ggcgagcgtg aagcggagcg catcggtgcc gaactgctcc atcacatgca atggatcgat
     1681 aacgttgcct ttcgacttgc tcattttttg cccttcggca tcgcggacca gggcatggat
     1741 gtagacttcg cggaagggga catcatccat gaatttcaaa cccatcatga tcatacgggc
     1801 gacccagaaa aacagaatgt cgaggccggt gaccagtgtc gaggtcggat aataggtctt
     1861 gagctcggga gtgtgctcag gccatcccaa tgtggaaaag ggccagaggg cagatgagaa
     1921 ccaggtgtcc aacacgtcgg ggtctcggat gaactcggtt ccgccgcaga cgtgacagga
     1981 tgccggcgcg tgcttcgcga caatcggctg ggccgtggaa aggatcacaa gacgcttggg
     2041 gatatgctca ggtccgtatg tgatgcccgc aacccgatgt tctgtctcga ctagcgactc
     2101 cttgttgcag ctgcggcaat accaggcggg aatttggtgt ccccaccaga tttggcgtga
     2161 gatgcaccaa tctttgatct ccctcatcca tccgaggtaa ttgttcgtcc atccttctgg
     2221 aatgattctg atgcgtccgt cttcaacggc ttggatcgca ggctcggcca ggggcttgat
     2281 cttcacaaac cactgagggg acagataggg ctcgacgatg gttttgcagc gatagcactt
     2341 tccgatcgcc atcttgtggt cgtcgacttt cgtcaagagt ccacggtctt tcagcgcttg
     2401 ctcgatcttg ggtcttgcct tggggacggg aagggttgcc actgcgtgaa ggacggcttg
     2461 ttcaacgccc gcggttgtga gtccctcggg atcaagcaga gcctgatgat cgaagatgga
     2521 atatcgtggc agtccatgcc gttcacccgc ttcaaagtcg ttgaagtcat gcgccggagt
     2581 gattttgacg acaccggtgc cgaattcgcg gtcgacgagg atcgcatcgc ccacgatcgg
     2641 aatcgtgcgg tgtgtgaggg ggagacggac tcgttgaccg atcaatcgat tgtaacgggg
     2701 atcctccggg tggaccgcca ccgcgctatc gccgaacatg gtttccggtc tggtggtcgc
     2761 gatagtgagc cggatcgttg gatcctgttc gagtggatag tcgatcgagt acagtttccc
     2821 tttgatctct tcgtgttcaa cctcgatgtc cgacagagcc gtgagacaac gcgggcacca
     2881 gttgatcaac cgctcaccac ggtaaatcaa cccatcttca tggagccgta caaagacttc
     2941 caggacggct tttgacagtc cttcgtccat cgtaaaacga aggcgttccc aatcgcagga
     3001 ctcaccgagc cgtttctgct gacggataat ggtatccccg gaagtggctt tccattgcca
     3061 gatgcgctcg atgaagcgtt cacgtccaag agattccctg gagatgcctt cggccaggag
     3121 ctgcttttcc acaacattct gggtggcaat cccggcatga tcggtgccgg gcagccacaa
     3181 cacgttcaac ccctgcatgc gtcgccagcg tatcaggatg tcttgcagcg aatgattcag
     3241 tgcgtggccg acatggagtg agcctgtcac attcggcgga gggatcacga tgcagtacgg
     3301 ttggccggaa tgggtcggcg aggcgtggaa atagcccttt tggttccaga cctgggacca
     3361 acgtgcttcg accgccttgg agtcgtaggt tttgtcgagt tggggtgtgc tcatgtgatt
     3421 gatctggtct atctatttga tctggtttgt ttcgtttatc tggcgggctt tttcagcatc
     3481 gcgcgtgaat cgcggggcat cttaacatgc gaatggtgac ctctcaaggc gaagccaggg
     3541 acattctcag cttgaggggt gtagagccgt gtgctataca gacccgacaa ggcgagggcg
     3601 aggggctcgt tccgcttcca ctttttacag tttggagaga ctatggccac agggcgtgaa
     3661 aaagaccgta agacgagaaa gaagcatcga aagaatgtcg cgcgggtgaa ggcactgacg
     3721 acggccagac ggagtaaggc gaagaagacc aaaaaggggt aagtcggatc gaaggcgaaa
     3781 gtcctacgcg gcttgtgtga ggcgtgtgat ctctgctttg atctgcgctt cggccaggtc
     3841 cggcacgagt cgctgaacag tctgttcgac ctgttctttg gccgtcgtcc ggacgagtct
     3901 ttcgactgtt tgtgaagtct gcttggcggc agcctgttga atctcttcct tcaccagtcc
     3961 ctcgatcgat ttgagttctc cctgaaccat atcggacagt gcggtgcgca cggcgttgag
     4021 ttcttcgcgg acgaattgat cgatgcgctc cttgatcagg ggactcgcaa tttcggacac
     4081 gctggttcga atgacgggtt tgaggaggga tatttcctgt gagaggatct tcgggagtgc
     4141 ggtcatgagt accggttcaa tcgcctgagt caaccgctct tccgagaggg atctccctaa
     4201 ctg
//
LOCUS       DATIPF010000435         4262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1308384, whole genome shotgun sequence.
ACCESSION   DATIPF010000435 DATIPF010000000
VERSION     DATIPF010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308384"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1549
                     /locus_tag="VLL06_10845"
     CDS             <1..1549
                     /locus_tag="VLL06_10845"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSS31505.1"
                     /translation="VCGEAEELWPQLVAEYEAGKMQRMYKMTSFPPLDRYKSPRVELL
                     SPDSYMTRQCSFTTRGCHFDCEFCSVSPFNGKTTRRRPVQEVVTELRNVQRWVRSELV
                     ERLRDEPMWRAFISAIRLRVGIEDGSIVAFVDDLHNSNRAYCRELWAALKPLKIKWGC
                     QSTMFLADDEEMVKLAAESGCVSVFVGMESLDEDALDETNKPFNRVQKFVREIKMFHD
                     YGIMVNPGIVFGFDSDDVTVFERAVDFLTKNQVELAYFNVLTPLPGTALFERFEKAGR
                     IFDKNWAKYDGKHVVFHPSRMTTEQLQDGFHWANHQFYSIPSIWKRLSGTSQRFLPRL
                     EMNREFRKLVKRTCPKGSVSPLAGVLRNLQAKLPVLDKEGLIPSALHALKQHFDPNAL
                     SAQQLMFQIKAKRHDKFAALFVDLDGTLDRLNALELIKRIKEAAKHARMDIIVNFEHL
                     KLATPDALKALMDSVAMKVAIPNVKVRFRKFKDAFESSLQGFSLSGLQGFSLAGLEHL
                     NEDSQDA"
     gene            1527..2804
                     /gene="lysA"
                     /locus_tag="VLL06_10850"
     CDS             1527..2804
                     /gene="lysA"
                     /locus_tag="VLL06_10850"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HSS31506.1"
                     /translation="MKTLRMHNFEYRHGELYCEQVPVSRIAKEVGTPCYIYSYTTLVR
                     HFRVYDGAFKSIPHVIAFAMKANSNLAIIRLMAQEGSGVDIVSGGELFRALKAGVPPS
                     KIVFAGVGKTAEEIREALKADILMFNVESSAELQAIHEVAASVGTKARVALRINPDID
                     PKTHPYISTGLKKSKFGIAADRALEEFTLASSLANIDVVGVHKHIGSQLTDVTPFVEA
                     VKKVVKLVDTLKAQGINIRYVNIGGGLGITYSDETPPLPQDLADAVSPLLKSLNVTLI
                     LEPGRVIVGNAGILVTKVLYRKDGEAKRFIIVDAAMNDLIRPSLYGAYHEIRPLSEAV
                     LQRTKHSVDVVGPVCESGDFLAKDRSLPEVNPGDMLAVMSAGAYGFVMASNYNSRPRI
                     PEVLIKDGDIHVIRTRETYDDLIKGETIPAFLG"
     gene            2846..3718
                     /gene="dapA"
                     /locus_tag="VLL06_10855"
     CDS             2846..3718
                     /gene="dapA"
                     /locus_tag="VLL06_10855"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HSS31507.1"
                     /translation="MFTGTLIAIVTPFRNGKVDERAFGDLIEWQIASGTNGIVPCGTT
                     GESATLSHQEHHRVIELTVEVVNRRVPVIAGSGSNSTAEAVSLTKHAKQAGADGALII
                     TPYYNKPTQEGLYRHYKMIAESVELPQILYNIPGRTGVNMLPSTVARLCGLQNIVGIK
                     EGSGSVQQASEIASTCGDRMTVLAGDDALTLPMMAVGGKGVISVTSNVVPSEMAQLVQ
                     AFLTGRIDEARRIHFALSPLFNALFYETNPIPVKTALGMMGKIDPELRLPLCAMATET
                     KDQLTRVLKDAGLI"
     gene            3740..>4262
                     /gene="dapB"
                     /locus_tag="VLL06_10860"
     CDS             3740..>4262
                     /gene="dapB"
                     /locus_tag="VLL06_10860"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HSS31508.1"
                     /translation="MIKVIVAGAAGRMGCRLVALIKDSTLLTLAGAIEGKGHLALGDD
                     AGDTAGCGRAGLPITDDVAVLLDRGEVVVDFSAPEASLQHLRTVAQHRRAMVIGTTGF
                     SAAQLEEIKSLASQVPCVVSPNMSVGVNLIYKVISEMAKTLGDDYDIEVIEAHHRLKK
                     DAPSGTALKIAEVL"
BASE COUNT          964 a         1097 c         1292 g          909 t
ORIGIN      
        1 cgtctgtgga gaagccgaag aactgtggcc gcagctggtt gcggagtatg aggcggggaa
       61 gatgcagcgc atgtataaga tgacctcatt tcctccgctg gaccggtaca agagcccccg
      121 tgtcgaattg ctgagccccg actcgtatat gacgagacag tgcagcttta cgaccagagg
      181 atgtcatttc gattgcgagt tctgtagcgt gtctccgttt aacggaaaga ccacgagacg
      241 tcggccggtg caggaagtgg tcacagagct tcggaatgtt cagcgatggg ttcgcagtga
      301 attggtcgaa cgattacgcg acgaaccgat gtggcgcgcc tttatctcag ccatccggct
      361 tcgggtggga atcgaagacg gcagcattgt cgcgtttgtc gatgatctgc acaacagcaa
      421 ccgtgcctat tgccgcgaac tctgggccgc gttgaagccg ctcaagataa aatggggctg
      481 ccagtccacg atgttcctgg ccgacgacga ggagatggtc aagcttgcgg ccgagagcgg
      541 ctgtgtgtcg gtcttcgtgg ggatggaatc gctcgatgaa gacgcgctgg atgaaacgaa
      601 caagccattt aaccgagtcc agaagttcgt ccgggagatc aagatgttcc acgattacgg
      661 gatcatggtg aatcccggga tcgtgttcgg attcgacagc gacgatgtga ccgtgttcga
      721 acgggccgtc gacttcttga cgaagaacca ggtggaattg gcgtacttca acgtgttgac
      781 gcccctcccc ggtacggcac tgttcgagcg tttcgaaaag gcgggtcgta tcttcgataa
      841 gaattgggcc aagtacgacg gcaagcacgt ggtgttccac ccgagtcgaa tgaccaccga
      901 acaattacaa gacggcttcc actgggccaa ccatcaattc tattcgatcc cttcaatctg
      961 gaaacgattg tccggaacga gtcagcggtt tcttccgcga ctggagatga atcgggagtt
     1021 tcggaaactg gtcaagcgaa cctgccccaa ggggtccgta tcgccgctgg caggagtgtt
     1081 gagaaatctc caagcgaagc ttccggtatt ggacaaggag gggttgattc ctagtgcgct
     1141 gcatgctctc aagcagcatt tcgatccgaa tgcgctctcg gcacaacaat tgatgttcca
     1201 gatcaaggcc aagcggcatg acaaatttgc tgcgctgttc gtggatcttg atggtaccct
     1261 cgatcgccta aatgccttgg aattgattaa gcggattaag gaagcggcca agcacgcgcg
     1321 gatggacatc atcgtcaact tcgaacatct caagctggcg acgcccgacg cgctgaaggc
     1381 gctgatggat tcagtcgcga tgaaggtggc aattccgaat gtgaaggttc gctttcgaaa
     1441 gtttaaagat gcgttcgagt cctccttgca gggcttctcg ctctcaggct tgcagggctt
     1501 ctcgctcgcg ggccttgaac atctcaatga agactctcag gatgcataac ttcgagtatc
     1561 gacatggcga gctctattgc gagcaggtac cggtcagtcg gattgcgaaa gaggtcggca
     1621 cgccatgcta catctacagc tacacgaccc tcgtgcgtca tttccgggtc tacgacgggg
     1681 ccttcaagag cataccccat gtgattgcat tcgccatgaa ggccaactcc aatctcgcga
     1741 tcatccggct catggcacag gaggggagcg gagtggatat tgtatctggc ggggagctct
     1801 ttcgggcgct caaggcgggc gttcctcctt caaagatcgt ctttgcgggc gtcggcaaga
     1861 ccgctgagga gattcgagag gccttgaagg ccgatatcct catgttcaac gtcgaatcgt
     1921 cggccgagct gcaggcgatc catgaggtgg cggcttcggt cgggaccaag gcccgtgtgg
     1981 ctctgcgcat caaccccgac atcgatccga agacccatcc gtacatttcg accggtctca
     2041 agaagagcaa gtttgggatc gccgccgatc gggcgctcga agaattcacg ctggcctctt
     2101 cactcgccaa catcgatgtg gtgggagtcc ataagcatat cgggtcgcag ttgacagatg
     2161 tcacgccgtt tgtcgaagcc gtgaagaagg tggtgaagct ggttgacacc ctgaaggctc
     2221 agggtatcaa catccggtac gtcaatatcg gcggtgggct tggcatcacc tactcggacg
     2281 aaacgccgcc gttaccacaa gatttggcgg atgccgtgtc accactgcta aagagcttga
     2341 atgtgaccct tattctcgag ccaggccgcg tgatcgtcgg caacgccggc attctggtca
     2401 cgaaagtgtt gtatcggaag gacggcgaag cgaaacggtt tattatcgtc gatgccgcga
     2461 tgaacgacct gatcagaccg agcctctacg gggcatatca cgaaattcgc cccttgtccg
     2521 aagcggtgtt gcagcggacc aaacacagtg tggatgtggt cggcccggtc tgtgagtccg
     2581 gcgattttct ggcgaaggac cggtcattgc cggaggtcaa cccgggcgac atgctggcgg
     2641 tgatgagtgc gggggcctat ggatttgtca tggcctccaa ctataattcg cgaccacgaa
     2701 taccggaggt gctgatcaaa gacggagaca tccacgtgat caggactcgg gagacttacg
     2761 acgatttgat aaagggtgag acgatcccgg catttctcgg ctgagaaatg agtgcgacgg
     2821 acgcgtcgca cgacaaagga gcaacatgtt tacaggtaca ttgatcgcca tcgtgacccc
     2881 attccggaat ggcaaagtcg acgaacgggc ctttggggac ctcatagaat ggcaaatcgc
     2941 cagcgggact aacggaattg tgccctgcgg caccactggc gaatctgcca ccctctcgca
     3001 ccaggaacac caccgtgtga tcgaactgac cgtcgaggtg gtcaacaggc gcgtccccgt
     3061 cattgccggg tccggttcca atagtacggc agaagcggtg tctctcacga aacacgccaa
     3121 gcaggcaggg gccgatgggg cgctgatcat tactccctat tacaacaagc cgacgcaaga
     3181 ggggttgtac cgccattaca aaatgattgc agagtcggtg gagcttccgc agatcctgta
     3241 taacattcca gggcggacgg gtgtgaacat gttaccgtcc actgtcgcga ggctgtgcgg
     3301 gctacagaat atcgtcggga ttaaagaggg gagtgggtca gtccagcagg cttcagagat
     3361 tgcgagtaca tgcggtgacc ggatgacggt gctggcagga gacgacgcgc tgacgctgcc
     3421 catgatggcc gtcggaggaa aaggggtgat tagcgtgacg tcgaacgtcg ttccctctga
     3481 gatggctcaa ttggtgcaag cctttttgac cggccgaatc gatgaagcgc gccggattca
     3541 tttcgccttg tcgccgttgt tcaacgcact cttttatgag accaatccga tcccggtaaa
     3601 aaccgcgctg ggcatgatgg ggaaaatcga tccggagctg cggctgccgc tatgtgccat
     3661 ggcgaccgaa acaaaggatc aactcacacg ggtcttaaag gatgcaggtc tcatttagcg
     3721 tgagggggaa aagtattaga tgatcaaggt cattgttgcc ggcgctgccg gtcgcatggg
     3781 ttgtcggctg gttgcgctga tcaaagattc caccctcttg acattggccg gcgcaatcga
     3841 agggaaggga cacctcgcat tgggtgacga tgcgggagat acagctggct gtggccgagc
     3901 aggccttccg atcaccgacg atgttgccgt gctccttgac aggggtgagg tggtcgtcga
     3961 cttttccgct ccagaagcat cccttcaaca tttacgcacg gtggcgcaac atcgacgagc
     4021 catggtgatc ggcacgacag ggttttcggc tgcccaactc gaagagatca agtcccttgc
     4081 gtcccaagtt ccctgtgtgg tgtcccccaa catgagcgtg ggggtcaatc tgatctacaa
     4141 ggttatcagc gagatggcga aaacgctggg ggacgattac gatattgaag tgattgaagc
     4201 gcatcatcgt ctcaaaaagg atgcgccgag tggaacggcg ctcaagattg ctgaagtgtt
     4261 gg
//
LOCUS       DATIPF010000436         3711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_878095, whole genome shotgun sequence.
ACCESSION   DATIPF010000436 DATIPF010000000
VERSION     DATIPF010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878095"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VLL06_10865"
     CDS             complement(<1..207)
                     /locus_tag="VLL06_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31509.1"
                     /translation="MMTRAEAVALRRTWKARAGTLTCLHHYLIVERIDPKRSTKHFLC
                     MDCGADVVRRFTQTVEERVDRASPR"
     gene            complement(227..364)
                     /locus_tag="VLL06_10870"
     CDS             complement(227..364)
                     /locus_tag="VLL06_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31510.1"
                     /translation="MLYYPFMFLSTNLMAFISIMLSLLVGMMALYLYNSGAFTRTARK
                     L"
     gene            complement(656..1093)
                     /locus_tag="VLL06_10875"
     CDS             complement(656..1093)
                     /locus_tag="VLL06_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS31511.1"
                     /translation="MTPTSEPRSTSILLIDASQNQRAYWAEQLKRCSAEYEIIEAPDS
                     ESGLALYRSRRIDCMVLELSLPDQGGFKTLVELVPIASRPQTAVIVLTLITHRGVWEL
                     AKQSGAYTCLAKKFTSGEDLDKAIQRAVAFVGQMPKEDRYQSI"
     gene            1888..2139
                     /locus_tag="VLL06_10880"
     CDS             1888..2139
                     /locus_tag="VLL06_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31512.1"
                     /translation="MLSMNGIIVLELLLGAALMGLIFTSFADTELIADPSSKSNQEPE
                     SDSVGATMKPGLAQTEISCQMYMSVLAGDYYWAKATGTS"
     gene            2224..2736
                     /locus_tag="VLL06_10885"
     CDS             2224..2736
                     /locus_tag="VLL06_10885"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSS31513.1"
                     /translation="MSTFFVTSIDVNVPVRTAYNQWTQFKDFPRFMEGLKEIRQLDDR
                     HLHWKAEIGGKDQEGDVEIVEQTPDQRIAWKNQAGAIITGGVVSFQQLSDSRSKVLLQ
                     LTYKPEDVVTHVGGANGMMSLHVQRDLERFKSFIESHGRESGGKRAGRGKAHIGDKAD
                     ITIPIQQRDG"
     gene            3341..>3711
                     /locus_tag="VLL06_10890"
     CDS             3341..>3711
                     /locus_tag="VLL06_10890"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HSS31514.1"
                     /translation="MRQFVMILAATIFVMVGAVNTTQARDKSGVLKGSDLIGMKVEGT
                     DGKKLGTIKDLVINPIDGDVDYAVLDYGGFLGVNDKYFAVPWEALKINDDRKKALLDV
                     TLVREQPHPPRRGEAPGDRAGP"
BASE COUNT          932 a          918 c         1038 g          823 t
ORIGIN      
        1 gcgaggagag gcgcggtcaa ctcgttcttc cacagtctga gtgaaccgtc tcaccacgtc
       61 cgcaccacag tccatgcaga gaaagtgctt cgttgaacgc ttggggtcga tccgttccac
      121 gatgaggtag tgatggagac acgtgagggt accggctcgc gccttccagg ttctgcgcaa
      181 tgcaacggct tctgctcttg tcatcatgag cgtgtcctcg cctgtatcac aatttccgag
      241 cggtcctggt gaacgcaccg gagttataca ggtacagggc catcatcccg actagaagcg
      301 agagcatgat cgagataaag gccattagat tcgtcgaaag aaacataaat ggatagtaga
      361 gcataaccac acccctctct attgcccgtc ctcttgaggg aggtcatggt cctatcgcct
      421 gcattagcag accaaagaca gacactctct cagagaaacc tacgagatca gaatgaagga
      481 gactggagaa tcgatcacgc tctgaaggat tgggaagcgc aagagatggg acagtcagcg
      541 ggctttatac ctcgcccctt caccctgggc gctatcggat cagaagtcaa gcaatactaa
      601 taatgacagg gtatggcagg tgcgatgaga gagagggaga gcagaaagtg ggatgttaga
      661 ttgactggta tcggtcttcc ttcggcatct gtcccacaaa cgccaccgca cgctgaatgg
      721 ctttatccaa gtcctcgccc gacgtgaact tcttagccaa acacgtgtat gccccacttt
      781 gtttggctaa ttcccacacg ccgcgatgtg tgattagtgt aaggacgatc accgcggtct
      841 ggggtctgct cgcgattggg actaattcga cgagtgtctt gaagccccct tgatcaggga
      901 gtgacaattc gagcaccatg caatcaatgc gccgagaccg ataaagtgcc agtcctgact
      961 cactatccgg cgcttcaatg atctcatact cagcggagca acgcttcagt tgttctgccc
     1021 aataggcgcg ctggttctgg ctggcatcaa tgaggaggat ggatgtagag cgaggttcgg
     1081 aggtcggagt catcgtatgg tattcccggc ttgaaataca ttgggggcga ttttacctct
     1141 tattgagggc cgatgtctgt aggcgattgc tgacaccgct acctagggca tcgcacatat
     1201 ccccggcttg ggaccatcca ggcaatctct gaccaccaac agcacgatcc ccgcaacatc
     1261 cccaccaatc cctgcagagt taacggtgcc tttccttccg cgccgataac cccagcgcca
     1321 tgtcatgttc taatttacaa gaatatgttg accgagagca ccccatgatc cctcccagca
     1381 taccgatggt gaggttcaac gacgaatgcg actcttttta aaaaaaagaa tagcaaagga
     1441 aggctgtttg tctagggacc ggtggaattg gagccgcatt ccatgcggta aacgaatgat
     1501 gcacagagtt tacacctggc ggtccttcgt cctacaacaa ctgttgattg catgaaggca
     1561 aaacaaaggc ccgactcctt tccgggtcga gccttgtact tcagattccg caggtcacac
     1621 tggtgggtct cttacagtgt ttccaagacg gttttctaaa gcagtcatca ctctactagg
     1681 tccctaacat agggccttct cgggaaaagc ctagtggagg aaccatgcct gcggaccgct
     1741 gggataagtg ggtacctagg gtacatcgcg aaacaaacaa ggcaagttac taggcccacg
     1801 tctgagctgt ctcatgttag atggaaggta gatggacggg tgtttcacct acgatgtcat
     1861 cgtcacatgt tcagaaagag gtgggtcatg ctcagtatga atgggattat cgtccttgaa
     1921 cttcttcttg gtgcggcctt gatgggtctg atattcacgt cattcgcaga tacggaactc
     1981 attgctgatc catcgtcgaa gtcgaaccag gagccggagt ctgatagcgt cggggctacg
     2041 atgaagccgg gcctggcaca aaccgagatt tcttgccaaa tgtacatgag cgtattagcg
     2101 ggagactact attgggcgaa agcgacaggc acgagctagc agggatactc atttggactc
     2161 aatagggctt ggaccgcgca gcctacatag gaggttcata tcgattcaca aaaaggaggg
     2221 cacatgtcga cctttttcgt gacatccata gacgttaatg tgcccgtacg gactgcctac
     2281 aaccaatgga cccagtttaa ggactttccg cgcttcatgg aagggctgaa agagattcgt
     2341 cagctagatg atagacatct gcattggaag gccgagattg gaggtaagga tcaagagggg
     2401 gacgtagaga tcgtagagca gacgcctgac cagcgaatcg cttggaagaa tcaagcgggc
     2461 gcgatcatca ctgggggcgt cgtgtcattc caacagcttt ccgatagcag gtcaaaggtc
     2521 ttgctgcaac tgacgtataa acctgaggat gtcgtcacac atgtcggagg ggcaaacgga
     2581 atgatgtctt tgcacgtgca gcgagatctg gagcgattca agtccttcat cgagtcgcat
     2641 ggtcgggaaa gcgggggcaa gcgtgcgggt agggggaagg cccatatcgg cgacaaggcc
     2701 gacatcacta tacctataca gcagcgcgat ggataaatga ggcgctcaga atgacagcac
     2761 atcgatcgct cgacgccgca acagacggag atgtagacac cagggcccat ggttcatgtc
     2821 gcccgttctc ggcatgaaat gctccagcgt agaagacgaa cccggtcggg gtcacttagt
     2881 tgtcggcgag ccccttccgt ctcttcaatc ggcggaacgt cttgttacct ccgtgggaat
     2941 atgatttacc tcatttttga gcaagcggct tgtataaaag gtcggtgaac ttgccaaaat
     3001 tgggaatcgt gattgatttc cctcgatggc agaattattt tggagaatcg atgcaccagg
     3061 cctcctggag ccggaggtgg cagaggagat cctgctggtg cgaagaccac gagattgctc
     3121 gggcgcgact catgatcgac ccgattatag gccgtaacga agtagtagtg aattggggga
     3181 atctggcagg tgaccatcct tgggccgtgc aggatcacga acgccacgac acactgccca
     3241 acctaggcta ccacccagct tcacagactc tctgttgaca ggaagagtgg ttaaggaatt
     3301 agcgatgaca tcctacgtcc gtgattagag gaggcgcgct atgcgacagt ttgtgatgat
     3361 tctcgctgcg actatatttg tgatggttgg cgcagtgaat acgacccaag ctcgcgacaa
     3421 gagcggggtg ctcaaaggaa gtgacctgat cggcatgaag gtcgagggaa cggatggcaa
     3481 aaaactcggt acgattaaag acctggtgat caatcccata gatggcgatg ttgattatgc
     3541 cgtcctcgat tatggaggat tcttgggtgt gaacgacaaa tattttgccg tcccatggga
     3601 agcattgaag atcaacgacg ataggaaaaa agctctgctg gacgtcactc tggtacgaga
     3661 acagccgcat ccaccgcgtc gcggtgaggc acctggcgac cgtgcaggcc c
//
LOCUS       DATIPF010000437         3630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_615309, whole genome shotgun sequence.
ACCESSION   DATIPF010000437 DATIPF010000000
VERSION     DATIPF010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615309"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1900)
                     /locus_tag="VLL06_10895"
     CDS             complement(<1..1900)
                     /locus_tag="VLL06_10895"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSS31515.1"
                     /translation="MEDQRYIALSPDPAKFSPAPVSSSVQVSLTTDRFPAWMERHQTW
                     IVSAVSVMTIVTLLCMGWAAYTVQQGLIQSRGHNLAQAATDAASKLDMLILERYRDIQ
                     LLSTAPITQGQNPDKLTTYLHSMVQAHPAYQWIGVTDSHGKIVASTDTSRTSLDRSES
                     RWFQLAHTITDVRILDARVSDESEGISGITVVAPLRSPDGRFLGAIAAIVGVPSLMHI
                     LDDTMQVLKNIEWTEESHLEYQLLNEKGDLLADSTRHEKDTLDLKRLGLPSATLVGIH
                     ARGFIEETHPLRRTAVITAYAQVPITHANPALRWGVLIHVDRDSILTPFRSFLLKLSF
                     FAILILLPLLGLVLGMIKALHGEWATAKREFQRATNAEAALQKRTEALHTLVVAAQTL
                     STQEDLDGLLYQLLHLAKENSGARYAALGISSDNSREPRPLLSAGIDETAAHAIRTLP
                     LEQGAQGPLGQKESPLRLAHLTEYWAALGLPANHAPLTSFLAVSIRCQGQFFGRLFLA
                     NKVTAQGVVPDFSELDEQVVLTLAAQAGTAIQNLQLLHDSKEQARKDSLTGLLNHSTA
                     FTVLTQELSRAQRDHYPVAVLIADLDYFKGINDTYGHPVGDLVIQETARRLRETARRS
                     DHVGRIGGE"
     gene            complement(2349..3095)
                     /locus_tag="VLL06_10900"
     CDS             complement(2349..3095)
                     /locus_tag="VLL06_10900"
                     /inference="COORDINATES: protein motif:HMM:NF040218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31516.1"
                     /translation="MNWVRAITAVCALWSTAAWGATLTWDVDSAPDLAGYRIYQCSQL
                     PCTLASGQASSLATLGKVTSFNIGTPAVTQYYFVTAYDLSNNESGGSNVATYIPAGAS
                     PTPPPPITPPPPSPPPVTSPPVPSAAIRATPVSLSFAATEGGANPASQTLSISNSGSG
                     TLNWTASDSAPWLRLSRSSGTNNGVIIVSVITGTTVVGTYNGTITLSATGIASVTVPV
                     AFTVDTTSVPPPASQTPPPPPNGLHISAVQ"
BASE COUNT          799 a          923 c         1091 g          817 t
ORIGIN      
        1 cttctccccc gatgcggcca acgtggtcag agcggcgtgc ggtttcgcgg aggcggcgtg
       61 cggtttcttg aatgacgagg tcgcccacag gatgtccata ggtatcgttg attcccttga
      121 agtaatcgag atcggcgatg aggaccgcca cggggtaatg gtctcgctgc gcccgcgaca
      181 attcctgcgt caagaccgtg aacgcagtcg aatgattgag caatcccgtc aacgaatcct
      241 tcctggcctg ctccttggag tcgtggagga gttgcaggtt ctgaattgca gtaccagcct
      301 gggcggcaag ggtcagaaca acctgttcgt ccagttcgct gaagtcgggc acgacaccct
      361 gtgcggtcac tttattcgca agaaagagcc taccgaaaaa ttggccctgg catcgtatcg
      421 acacagcgag gaatgacgtc agaggcgcat gattggcagg aagtccgagg gcagcccagt
      481 attctgtgag atgtgccagg cgaagggggc tctccttttg cccaagaggc ccctgcgctc
      541 cctgttcgag cgggagtgtc cgaatggcat gggctgcggt ttcatcgatt ccagcgctca
      601 ggagcggtct tggctcccgt gaattgtcgc tggagattcc taatgcggca tagcgtgcgc
      661 cgctgttctc ctttgcgaga tgaagaagtt gatacagcag tccatcaagg tcctcctgcg
      721 tggataatgt ctgtgcagca acgaccaacg tatgcaaggc ctccgtccgt ttctggagcg
      781 ccgcttccgc gttggtcgct cgttggaatt cccgtttcgc ggtggcccat tctccgtgga
      841 gagccttgat cattccgagc acaagcccaa gcaaggggag gagaatgaga atggcgaaaa
      901 acgataattt caggagaaat gatcgaaagg gggtcaggat gctatcccgg tctacatgaa
      961 tcaagacgcc ccatcgtagg gccgggttcg catgggtgat aggcacttgc gcataggcgg
     1021 taatgacagc ggtccgccgg agaggatgag tctcttcaat gaagccccgg gcatgtatcc
     1081 ccacgagtgt tgcagaggga aggccgagcc gtttcaaatc gagcgtgtct ttctcgtgtc
     1141 gcgttgagtc ggcgagcaga tctcccttct cattgaggag ctgatactca agatgtgact
     1201 cttctgtcca ctcaatgttt ttgagcacct gcatcgtgtc gtcaaggatg tgcatcagtg
     1261 aggggacgcc cacgatggcc gcgatcgctc cgagaaatcg tccgtctgga ctgcgcaggg
     1321 gggcgacaac cgtgatcccc gagatccctt cagattcgtc actcacgcgg gcgtctagaa
     1381 tcctgacatc cgtgatggtg tgtgccagtt ggaaccagcg actctcgctc cgatcgaggg
     1441 aggtcctaga tgtatcggtg gacgcaacga tcttcccgtg tgaatcagtg acgccaatcc
     1501 actgataggc cgggtgggcc tgcaccatac tatggagata ggttgtcagc ttgtcaggat
     1561 tctgaccttg ggtgatcgga gcagtggaga gcaactggat atcgcggtat cgctcgagga
     1621 tcaacatgtc caatttgctt gcggcatccg tggccgcttg cgcgaggtta tggcctctgg
     1681 attgaatcaa tccttgttga actgtgtatg cggcccatcc catacacagc agcgtcacga
     1741 ttgtcatgac agagactgcg gacacaatcc aggtctgatg acgctccatc catgccggaa
     1801 atcggtctgt cgtcagggag acctgaactg aactacttac aggggcagga gaaaacttgg
     1861 cagggtcagg gctgagggca atgtagcgtt ggtcttccat ttaagtagtg ctcgaggccg
     1921 cggccgatgg ctgcatccat cataaagtaa gtcataggcg aagcaaggaa agtactgatt
     1981 gtcgccccgg ttaaaattcg catatcgcgt ctgaatgcaa tagatctgtg aaagtgaagg
     2041 cttaggaggt gcaggaggac acggcgcttc gggcatgttc tggcggaatg ggcgagcctt
     2101 tcctgaccga gcgtgcgaaa gaaggcatcg gactcctccc tctgtagagt gattggagca
     2161 agagtatcga gtcagcttgg agagaccgtc aggggtggct cgcctgacgc aggcagcagg
     2221 ggtatcgtac ctgttctgtg cggcattaat ccccaggtag ggagagtctg tactcggaat
     2281 gaatcccgcc agtcttcctg gacccctctg ctcccaaact cattcctggt ccaactatcg
     2341 gatagatttt actgcaccgc actgatgtgc aggccatttg gaggcggtgg gggtgtctgg
     2401 cttgccggcg gtggcactga ggttgtatcg actgtgaagg ccaccggtac agtgactgac
     2461 gcgatcccgg tggcactgag cgtaattgtg ccattgtagg ttccgacgac ggtggtacca
     2521 gttatcacgc tcacaatgat caccccattg tttgtgccag aactccggct cagcctcagc
     2581 cagggcgcac tatcactggc ggtccagttg agcgtgccac tgcccgagtt gctgatgctt
     2641 aaagtctggg acgcaggatt ggccccaccc tctgttgcag caaaagaaag acttacggga
     2701 gtcgctctga tggccgctga cgggaccggt ggggaagtga ctggaggggg cgacggtggc
     2761 ggaggtgtga ttggcggagg cggggtcggc gaagctccgg ccggtatata ggtcgcgacg
     2821 ttgctacccc ccgactcatt attggaaaga tcataggccg tcacaaagta gtactgagtc
     2881 actgccggcg tcccgatatt gaaactcgtc acttttccca aggtggcaag ggaggatgcc
     2941 tgaccagatg cgagtgtgca gggtagctga ctgcattgat agatccgata gcccgccaga
     3001 tccggtgcac tgtcgacatc ccacgtcaac gtggcgcccc atgcagcggt agaccacaat
     3061 gcacacacag ccgtgatggc tctcacccaa ttcatacgga tgctccttcg tgtatatctc
     3121 ccgaccgcag gcacactatt tccggagtag taaatttctc tcatgaaaga tgtcatgacg
     3181 ggttgcttgt ctgattctgt catctgaaga gaatacgatc cggtgaccca ttcactccgc
     3241 gcctgtggtt actacgcaaa gcacatgccg cgattggaaa gggatgatca gaacaaaaac
     3301 caagaaatca gctgtgattc agcgagggtg agaggatcag agacggtgaa gtgtagagag
     3361 actaaaggag gtatcggcgg atccctgaag taatcctagt atttctcatg agatagactc
     3421 atgtagtcga taccctacag gtacggcagt aagaagaacg gtgtgagagc ctgctggtta
     3481 ctctggtcag ttcggtgtgt ctggtctgtc aattctatcc ggtttatatg attagtttct
     3541 tgtaaccaaa caaccaagac tggctagatg aaccggaact acctctcccg tcagctggcc
     3601 tgttgaagga tgcgttgctc atcaggaagc
//
LOCUS       DATIPF010000438         2969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1308722, whole genome shotgun sequence.
ACCESSION   DATIPF010000438 DATIPF010000000
VERSION     DATIPF010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308722"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1871
                     /locus_tag="VLL06_10905"
     CDS             <1..1871
                     /locus_tag="VLL06_10905"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HSS31517.1"
                     /translation="LLVTGYRWTLAVAGTVMGLLLVVLLLVDMGYYHFNHQRLNFVFY
                     EYVSDLVNQWKETGLQGSQAAEQTGAELHDGGKWTGRVVGFILLQALAVIGWWFCFTR
                     IVRPALRRLNRGTVLASNVIFLVGFVGGATGFHYQGPYAIRLTDISSSAYYVLAQNPI
                     LYAGEGLRAIVETKQKSGDSEKLNEMPLDEALRVAQETLGHNTIFPYPQYPFVHERFS
                     TPDVQFRRQANVLMILVEGLDRRFLNKTVGGIRLTPYLDHFKEDSLYFEHFFANGVQT
                     ARGLFASFCSYYPRQGTAAMKTRYMHDYVCIPSVLREHGYRTEMVIGYHRDLNRLHLF
                     MSRNGLHQLFDRSDFPPGIEEIGMSASVGKPDGALLDLMRTRVQALRANGKPYFLAAM
                     TIGMHHPFAVPQTHPDIQHLKAEPDGYLAALRYLDLELERVLTGMKRDGLLHDTVVLI
                     LGDHGRHEKVGHTDLENQVGHFMTPLFLWVDDTLREPGSYRPRTVTTVASQVDLLPTI
                     LGLNGMNPRLSPSLGHDLSCLLASDCLDNNMAFLSSVYDDLIGLADHEGFLLYSLRSH
                     ALRRADLNLKEAAVPVEPTDPAIALRYRRMLGLYVSANTILNQNKIWSWKEFGQKL"
     gene            1844..2629
                     /locus_tag="VLL06_10910"
     CDS             1844..2629
                     /locus_tag="VLL06_10910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSS31518.1"
                     /translation="MERIRAEAVTANVPKVSVYVIAYNDEPNMRACLESVAGWGDELI
                     VVDSHSTDRTAAISREFTDKVHQLDFHGFGRLRNEAVALTTHEWVFSLDSDERMTPEL
                     KEEIRLVLDRGPDADAYFVPRKNYFLGRWIKHCGWYPDYRQPQLFRKGRFRYREELVH
                     ESFDCDGPVGFLQSPALQYPFRDIDHYVAKQDRYSDLMARRMADQGRRFSSHQLITHP
                     LGACLKMYVQHAGFLDGMPGLILSGLYGYYTFMKYAKFWELSK"
     gene            2720..>2969
                     /locus_tag="VLL06_10915"
     CDS             2720..>2969
                     /locus_tag="VLL06_10915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSS31519.1"
                     /translation="MLSIIVAIHNQLGHNQLFLEGIRRYTTGPYEVIVIDNHSTDGSA
                     EFFESNRCRVIRNDRNRCYPESMNLGSNVASGEFLCHIN"
BASE COUNT          638 a          786 c          843 g          702 t
ORIGIN      
        1 ggctcctggt gacggggtac cgttggaccc tggccgttgc cgggactgtc atgggactct
       61 tgttggtcgt actgctcctc gtggatatgg ggtattacca cttcaatcat caacgcttga
      121 attttgtctt ttatgagtac gtcagcgatc tcgtcaatca atggaaggag acaggccttc
      181 aaggatccca agctgcagag caaaccggcg cagagcttca cgatggtgga aaatggaccg
      241 gaagagtcgt gggttttatc ctgctccagg cgctggcggt catcgggtgg tggttctgtt
      301 tcacgcggat agtgagaccg gcgctcagaa ggttgaatcg agggacagtg cttgcgtcca
      361 acgtgatctt cctagtggga tttgtcgggg gggcgacggg gtttcattat caaggtccgt
      421 atgcgatccg tctcaccgat atcagcagct cggcctatta cgtgctggcg caaaatccca
      481 ttctctatgc cggtgaaggg ttgcgcgcga ttgtcgagac aaaacagaaa tccggtgact
      541 cggagaaact caatgagatg ccgttggatg aagctctgcg cgtggcacag gagacgcttg
      601 ggcacaacac gatatttccc tatccgcagt acccttttgt gcacgagaga ttctcgaccc
      661 ccgatgttca gttcagacgt caggccaatg tgctcatgat cctcgtggaa gggctggatc
      721 gacgatttct caataagacg gtcggaggca tccgcctcac gccctatctc gatcatttca
      781 aagaggacag cctctatttc gagcatttct ttgcgaacgg ggttcaaacg gctcgaggcc
      841 tcttcgcgag cttctgttcg tattatcccc gccaaggcac ggcggcgatg aagacgcgct
      901 atatgcacga ttacgtctgc attccatcgg tattgcgtga gcatggctac cggacggaaa
      961 tggtgatcgg ttatcaccgg gatctcaacc gtctccactt atttatgtcc cgaaatggcc
     1021 tccaccaatt gttcgatcgg agcgatttcc ctcctgggat tgaagagatc ggaatgtcgg
     1081 ccagcgtcgg caagcccgat ggggctttgt tggatctgat gcggaccaga gtccaagcac
     1141 ttcgggccaa tgggaagccg tattttctcg cggccatgac gatcgggatg catcacccct
     1201 ttgccgtgcc gcaaacccat ccggacatcc aacacctgaa agcggaaccg gacgggtatc
     1261 ttgctgcgct gcgatatttg gacctcgaat tggaacgtgt gctgacgggt atgaagcggg
     1321 atggactgtt acacgatacc gtcgtcttga ttctgggcga ccacggccgc cacgaaaagg
     1381 tcggacacac agaccttgaa aatcaagtcg gccattttat gactcccttg ttcctgtggg
     1441 ttgacgacac gttgcgggag ccaggatcgt atcgtcctcg aacggtgaca accgtggcca
     1501 gtcaagtaga cctcctcccc acgattctgg gcctgaacgg catgaacccg cgtctttccc
     1561 cctcgttggg gcatgacctc agttgcctgc tggcgtctga ttgcctggac aacaacatgg
     1621 catttctgag cagtgtctac gacgatctga tcgggttagc cgatcacgag ggattcctgc
     1681 tgtactcgtt gcgcagccac gcattgcgcc gggctgacct gaatctgaaa gaagccgccg
     1741 tgccggtcga accgacggat ccggcgatcg cgcttcgcta tcgccggatg ctggggctgt
     1801 atgtcagcgc caacaccatc ctcaatcaga acaagatctg gtcatggaaa gaattcgggc
     1861 agaagctgtg acggccaatg ttcctaaagt atcggtgtac gtgatcgcat acaacgacga
     1921 acccaatatg cgggcatgcc tcgagtctgt ggcaggctgg ggtgatgagt tgatcgtcgt
     1981 ggactcgcat agcaccgatc ggactgcggc aatcagccgg gagtttaccg ataaggtcca
     2041 tcaactcgat ttccacggat tcggacgatt gcgcaacgag gccgtcgcac tcacgaccca
     2101 tgaatgggtc ttcagtctgg acagcgatga gagaatgacc cccgagttga aggaagagat
     2161 tcgattggtg cttgaccgcg gaccggacgc ggatgcctac tttgtgcccc ggaaaaacta
     2221 ttttctgggt cgatggatca aacattgcgg ctggtatccc gactatcggc aaccgcagtt
     2281 gtttcgaaag ggccgctttc gttatcgtga agagctggtt catgaaagtt tcgattgcga
     2341 tgggccggtc gggtttcttc agagtcccgc actgcaatat ccgtttcgag acatcgatca
     2401 ctacgtggca aaacaggacc gttactcgga tttgatggcc cgtcgtatgg cggatcaggg
     2461 ccgtcgattt tcttcccatc agttgatcac ccatccgctc ggcgcgtgcc tcaagatgta
     2521 tgtgcagcat gccggtttcc ttgacggcat gccgggcttg attctctccg ggctctatgg
     2581 ctattacacg ttcatgaagt atgcgaagtt ctgggaactg tcgaaatgac ctgcatgcat
     2641 cctgaacctt cgttggtacc gggaagtgtt ccgccgtggg ttttgacaat cacgtgagag
     2701 gtcggacgga cctcgcatca tgttgagtat catcgtcgcc attcacaatc agctcggaca
     2761 caatcagctc ttccttgaag ggatccggcg ctatacgacc ggtccgtacg aagtcattgt
     2821 catcgacaat cactcgacgg acgggtccgc ggagttcttt gagtcaaaca ggtgccgtgt
     2881 gattcgaaac gatcgaaacc gctgctatcc agaatcgatg aatctcggtt ccaacgtggc
     2941 aagcggagag ttcctgtgcc acatcaaca
//
LOCUS       DATIPF010000439         5416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_615730, whole genome shotgun sequence.
ACCESSION   DATIPF010000439 DATIPF010000000
VERSION     DATIPF010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5416
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615730"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VLL06_10920"
     CDS             <1..668
                     /locus_tag="VLL06_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSS31520.1"
                     /translation="IPGPIFANVVLADEINRTTPKTQSSLLEAMSEAQISVDNQTHLL
                     EQPFMVIATQNPAEYHGTFPLPESQLDRFLMRLCIGYPALEEERKVLARPHSLHPAEM
                     LEPVLSAHEVLALQARVDQVHMEESLTDYLLAIVQATRQSELLSLGVSTRGALGLSKA
                     AKALAVVRGRDYCLPDDIKELAPIVLSHRVMLNRTHGMRTQSFEQAERMVLDIVETVP
                     VPV"
     gene            713..1852
                     /locus_tag="VLL06_10925"
     CDS             713..1852
                     /locus_tag="VLL06_10925"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSS31521.1"
                     /translation="MKHPLLNLRTSPVFSYIWGMSRRRSIRLTSEGTRFLLFTFGIGL
                     AAINTGNNLFYLLLAMMLSLIVISGLLSEHCLRRLEFRRHVPDLIMANEPTTLTLSVT
                     NRNRHLPSFSLRLSDVVEGQDVDRGLAIHLLPPQSSVLLTYPLLATTRGWIRFEGIRV
                     QTLFPFGLFLKKGLYSMEAHLLVSPPIKPLTLRFVDELVTEGQGESLPRRGDGSQLYN
                     LRFYQPGDDSRAIHWMTTARTSQLTVRETESEDQRRITVVLSIVAPEECEPLFERSVT
                     FVASLLWQLTERAYPVRLIVGTEDSGLGLGSAHLLAMLRLLALCERQPPETGDHDEHS
                     LFTYDGEGSYTLAVVPWSNQAAPANTMQADRVLHAAQLEELTHAF"
     gene            1842..4034
                     /locus_tag="VLL06_10930"
     CDS             1842..4034
                     /locus_tag="VLL06_10930"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HSS31522.1"
                     /translation="MPFDQAFAVSSVLLAATAFSGLVLAQSVPLWLALPTALILIMSL
                     LHAGGVLFLRRAIAQITISPTLWNVMLISAFVILLIDLTVISRDLLPAGIHFLVVLLG
                     IKLLTLQQRRDYRHLYAISLMAILASAALTTDAWYVPIFLLYMLAAVWTLLLYHLTQE
                     TGEIPAVGIPPSPAACHATYPSRITHRFFWLTNGTAVLTVALTLVIFFLIPRIGAGVL
                     QKTSGEALRTTGFSDQVDLGTIGSVKQDPQIVMRVELPDQPFLEKDRFYLRGLAYDQY
                     NGRSWNHSGTRRRSLSFVADGTFLARPAGSRPSNSLVGAIRQDILLEMLDTSVLFAAP
                     FAELVSGEFLTVQTDVMGGLHLPVPPSSRIRYSVTSYVPQLAANERTAQTLAYPDSIR
                     SHYLQIPMESQKVADLAHRVTQQAVTPFEQTLAIQQHLLENYRYSLENDTATLNHPLD
                     EFLFMRKTGYCEHYATAMVVMLRTVGIPARLVTGFLATEWNEYGGYFTVRQRDAHAWV
                     EVYFPHSGWITMDPTPTVNAAVTSSRWEAFSRLMASVGLQWDRLFVHYSAKDQLAVVR
                     GVREGSDAVRERVSQWTSSVSAPIRQAFGRLAKMAQTFHPGMLGPITVVIIVGLALLI
                     LVLRDRIGLWAATHVPITHPQLAIVQLYTRMLRMLDRHGMKKPPAATASEFSKLIERD
                     WRAASPIVADVTALYHQGRFSQTPLTPGELSRAVEQLGRLQSLTHTVR"
     gene            complement(4438..4512)
                     /locus_tag="VLL06_10935"
     tRNA            complement(4438..4512)
                     /locus_tag="VLL06_10935"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4478..4480),aa:Gly,seq:gcc)
     gene            5040..>5416
                     /locus_tag="VLL06_10940"
     CDS             5040..>5416
                     /locus_tag="VLL06_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HSS31523.1"
                     /translation="MFLSRRQFLKVSVGTVAAVAVADQVLALTALQPVIEVGNPLGDY
                     PDRSWERVYHDQYRYDSSFTFVCSPNDTHACRVRAFVRNGVVMRVEQNYDHQTYEDLY
                     GNRGTFAHNPRMCLKGFTFHRRV"
BASE COUNT         1162 a         1556 c         1410 g         1288 t
ORIGIN      
        1 ttataccagg accgatcttc gccaacgtcg tgctggccga cgagatcaat cggacgacgc
       61 cgaagaccca gagcagcctc ttggaagcga tgagtgaagc acagatttcc gtcgataatc
      121 agacgcatct gctggagcaa ccatttatgg tgatcgccac gcagaatccc gcggaatatc
      181 acgggacatt tccccttccg gaatcccaac tcgatcgatt cttaatgcgg ctctgcattg
      241 gatatcccgc gctcgaagaa gaacggaagg tgctcgcccg tccccattcg ttgcatcccg
      301 ctgagatgct ggaaccggtg ctatcagccc acgaagtctt ggcattgcag gcccgcgtgg
      361 atcaggtcca catggaagaa agcttgacgg attatttgct cgcaatcgtc caagcgaccc
      421 gccagagcga gctcttgtcc ctgggggtca gcacacgcgg agcgctgggg ctgagcaagg
      481 cggccaaggc attggcggtt gtccgcggac gcgactattg tctccccgat gacatcaagg
      541 aactggcgcc gatcgtgctg tcccaccgcg tcatgctgaa tcggactcat ggcatgcgga
      601 cacaaagctt tgaacaggca gagcgtatgg tgctcgacat cgtcgagacc gtgcccgttc
      661 ctgtctagcc agtcacggat ggatgttccg gctaactgga tgatcgacgc gtgtgaagca
      721 cccactcctc aatctccgca cctccccggt cttctcctat atttggggca tgtcccggcg
      781 gcggtcgatc cggctgacgt ctgaagggac cagatttctt ctcttcacat tcgggatcgg
      841 gctcgccgcc atcaataccg gcaacaatct gttttactta ctcctcgcga tgatgctcag
      901 ccttatcgtg atctccggat tgctctcgga acactgtctt cggcgattgg aatttcgtcg
      961 acatgtaccc gatctcatca tggccaacga gccgaccacc ctcacgcttt cggtcacgaa
     1021 tcgaaaccgg cacctaccca gcttttccct gcgcctatcg gatgtcgtcg aaggccaaga
     1081 tgtcgacagg ggtcttgcta ttcacctcct gcccccgcag agttctgtgc tcctgaccta
     1141 tccactgctt gccacgactc ggggctggat ccggttcgaa ggcatccggg tgcagacgtt
     1201 gtttcccttt ggtctattcc tcaagaaggg gctctattcc atggaagcgc atctgctcgt
     1261 cagccctcct atcaagccgc tcaccttgcg attcgtggat gaactggtca ccgaggggca
     1321 aggagagtct ctcccacgac gaggagacgg gagccaactg tacaaccttc gcttctatca
     1381 accaggcgat gattcacgag ctatccattg gatgacaact gcccgtactt ctcagctgac
     1441 cgtgcgggag accgaatcgg aggatcaacg acgtatcacc gtcgtcctct caatcgtggc
     1501 gcccgaagaa tgcgaacccc tctttgaacg aagcgtgacg ttcgtggcat cacttctctg
     1561 gcaattgaca gagcgagcct accccgtgcg actcatcgta gggacagaag attcaggcct
     1621 cgggctagga tccgctcatc tattggcgat gctgcgtctg ttggcgttgt gcgaacggca
     1681 gccacctgaa accggtgatc acgacgaaca ctccctgttc acgtacgatg gtgagggtag
     1741 ctacacactg gcggtcgtac cctggtcgaa ccaggctgca ccggcaaata cgatgcaagc
     1801 tgatcgagtg ctccatgccg cacaactcga agaattaaca catgcctttt gatcaagcct
     1861 ttgctgtcag ctccgtcctc ctcgccgcca cagcatttag tgggctcgtg cttgcgcaga
     1921 gcgtcccact ctggctcgcc ctccccacag ccctcatcct cattatgtcg ctgcttcatg
     1981 caggaggggt actgttcctt cgccgtgcga tagcacagat caccatctcg ccgaccctgt
     2041 ggaatgtcat gttgatcagc gcctttgtca tattgttgat cgatctcacg gtcatatcac
     2101 gagaccttct cccggcgggt atccactttt tggtcgtgct gttgggtatc aagctactca
     2161 cccttcaaca acggcgtgac tatcggcatc tttacgcgat tagcctcatg gcaatcctgg
     2221 cttctgcggc actgacgacc gatgcatggt atgtcccaat ttttttgctg tatatgctcg
     2281 cggctgtctg gacactcttg ctgtatcact tgacccaaga aacaggcgaa atccctgccg
     2341 tcggcatacc tccaagcccg gcagcctgcc acgcgactta tccaagtcgt atcacgcacc
     2401 gcttcttttg gttgacgaac ggaaccgctg tcctgacggt tgccctgacg ctcgtcattt
     2461 tcttcctcat cccccgtatc ggtgcgggcg tattgcaaaa gaccagcggg gaggcactgc
     2521 ggacgacggg gttttccgat caggttgacc tggggacgat cgggtcggtc aagcaagatc
     2581 ctcagatcgt catgcgggtt gaactaccgg accaaccttt tcttgaaaaa gatcgctttt
     2641 acctgagagg cctcgcctac gatcagtaca atggtagatc atggaatcac agcggaactc
     2701 gccggcgatc gttgagtttc gtcgctgatg gaacgtttct cgctcgccca gccggcagcc
     2761 gtccatcaaa cagcctggtg ggagcgatcc gccaagatat cctgctggaa atgctcgata
     2821 cctcagtttt gtttgcggcc ccattcgctg aactcgtgag cggcgagttt ctcaccgtac
     2881 agactgatgt catgggagga ctccacttgc cagttcctcc ttcttcccgt atccgatact
     2941 ccgtcacctc ttacgttccg cagctcgccg cgaacgaacg aaccgcgcag accctggcct
     3001 accccgattc aatccgttcc cactaccttc agattcccat ggaatcgcaa aaagttgcag
     3061 acttggcgca tcgcgttact cagcaagctg tgaccccctt cgaacagacg ctggcgattc
     3121 agcaacatct cttggagaat tatcgttaca gccttgaaaa cgacacggcc acactcaatc
     3181 atcccctcga tgaattcctt tttatgagaa aaaccgggta ctgtgaacat tacgcgaccg
     3241 ccatggttgt catgttgcga actgtaggca ttcccgctcg tcttgtgact gggttcttgg
     3301 cgacagaatg gaatgaatac ggaggctatt tcactgtacg ccagcgagat gcccacgcct
     3361 gggtcgaggt ctactttcct cactcaggct ggattaccat ggatcccacc cccaccgtca
     3421 acgcagcagt gaccagctcc cgttgggagg ctttcagccg actcatggca tccgttgggt
     3481 tacagtggga tcgcctattc gtgcattata gtgcgaagga tcagctggca gtcgtccgtg
     3541 gagtacgaga aggcagtgat gcggtccgtg agagagtgag tcagtggacg tcgtcggtga
     3601 gtgccccgat tcgtcaggct ttcggacgcc tggcgaagat ggcgcagacg tttcatcctg
     3661 gtatgttggg accgatcacg gtagtgatta tagtcgggct ggccttgctc atcctcgtgc
     3721 tacgggatag gattgggctc tgggccgcca cacatgttcc cataacgcac ccgcagcttg
     3781 cgatcgtcca gctctatacc cgcatgcttc gcatgctgga cagacacggt atgaagaaac
     3841 ctccagccgc tacagccagt gaattttcta aactgattga acgggactgg agggcagcca
     3901 gccccatagt ggctgacgtt actgcgctct atcatcaggg acgatttagt caaactccgc
     3961 ttactccggg ggaactttct cgggcagtcg aacagttggg gcggctgcaa tccctcacac
     4021 acacagttcg ctaatcccct atcgctgcat cgtctcactc ccgtccgggc ctggctaggg
     4081 ctacaaccgc tcctcattcc tccaatacgt tcgacgagga attgggacgg tcggctctcc
     4141 caaagcccga tagaaagcca ttagcgagcc cattcgtcag gtgggacatg ggaacggcat
     4201 ggttctgctt caccaaattc atggcaccca aggtgctggc tatcgcacag gaaaagatag
     4261 ccacgcttgg tcgaagcacg tgggctgcga gaataaagga cggaaacaaa cctcccgctg
     4321 actggtgtta gcacgagtgc tatggagata agtactccga tcaggaaacg cgatgaagct
     4381 cctcatcgcg tgaagtacgg agaaaaggga ggcattgtga gtagacgacc gaatatttgg
     4441 agcgggaaac gggatttgaa cccgcgaccc tcgccttggc aaggcgatgc tctaccactg
     4501 agctattccc gctcgatcac aattggtcag aggggtggga ttctactgat gcgagaacaa
     4561 agctgtcaag caatcaagta ggtcccgctc atcattgtta tcttgttacc ttcgttacaa
     4621 taggccaatc atgctaccaa gtggtaacgc acgaatatta ttatcaagta tttgatttat
     4681 tgcacattta tcgcatgaaa gcatgtctgc acgtcgcgaa tgatacaaca tatactcagt
     4741 gatatgtatc tgggtcattt tcgtcattcc tagttgaaag agactttaaa ttataattca
     4801 atcacttact tcatatcgca taaacatgta tgtctgctta ttgtggtgcg catattgcgt
     4861 gcgcacatgt ctcacgatga ttgaattgca atacaaactg atagtctaac catgtttcat
     4921 cgtccgaatg acttgctcgc tttttgcatc tggtggctct ctagaggctc aaactttaca
     4981 atgtgtccgt gacggtgcgt gggcttgatg gaagccctca actggaggag gtgggtgtaa
     5041 tgtttttgtc acgtcggcag tttctcaaag tttcggtcgg gaccgtggcc gccgtcgcgg
     5101 tggccgacca ggttctagcg ctgaccgcgc ttcaaccggt catcgaagtc gggaacccgt
     5161 taggcgacta tccagaccgg tcctgggagc gggtctatca cgaccagtat cgctacgatt
     5221 cgtcttttac atttgtctgt tctccgaacg acacgcacgc ctgccgggtc cgggcctttg
     5281 tccgtaatgg tgtggtgatg cgggtcgaac agaactacga tcaccagact tatgaagacc
     5341 tctacggcaa ccgcgggacc tttgcgcaca atccgcggat gtgcttgaag ggctttacct
     5401 ttcatcgccg cgtcta
//
LOCUS       DATIPF010000440         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1760976, whole genome shotgun sequence.
ACCESSION   DATIPF010000440 DATIPF010000000
VERSION     DATIPF010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760976"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VLL06_10945"
     CDS             <1..397
                     /locus_tag="VLL06_10945"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HSS31524.1"
                     /translation="IVLAILGIMSMLAGPSYHTVVARAQARSVTAEIASELRLARQLA
                     MARRERLRVIFDREGRTITLRRADAEDILHVYQYADKGVVVDEPTAGPELLFHPSGRS
                     ASPTTICVRDSQGREITFTVSITGRVSVS"
     gene            394..885
                     /locus_tag="VLL06_10950"
     CDS             394..885
                     /locus_tag="VLL06_10950"
                     /inference="COORDINATES: protein motif:HMM:NF041940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor X domain-containing protein"
                     /protein_id="HSS31525.1"
                     /translation="MTRDRLCRAEGGAGFAEVFVAMALTMIGLAGAMGAFQTAERSLR
                     MGMLATRALAMAESRIEAKRAARWDRLLLDDLNYDGISDLVMHDDGAGGDVLAGDGMY
                     SGSWDQDGVHLIWTVTPSHSGSLSASGHVLIDARAVYAADEGPHEVRIGTLRANPVFV
                     GGP"
     gene            887..1651
                     /locus_tag="VLL06_10955"
     CDS             887..1651
                     /locus_tag="VLL06_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31526.1"
                     /translation="MPSLGVDGMQTGQRMSSACHRMRTEAGTSLTELMCAMAAGLVVL
                     GATLQALSYFQHRFMKQQHEVAQQQDLRLGLEVLEEELRLAGSGSLTITASDTVEFSA
                     NLQGLSTTVTAASEIGQTALSVEDGRGWEDRKTIVACWAEHCETLALAHDGQRRVLTV
                     TQPLTRVIPSGAFVFLLNHVRYYSRLDDQGVLRLLRQVDGGASVMVRDIREVRFSYWD
                     ENGQAVTQPAFVKLVVVEVMMSDQGIRAVRGISLRT"
     gene            1693..>2812
                     /locus_tag="VLL06_10960"
     CDS             1693..>2812
                     /locus_tag="VLL06_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31527.1"
                     /translation="MGERGASTFMNQCDRGHVLLAALMLIFLLGVVGMTSLYLAGQDG
                     PGVSAMREDKLAQQLADGATDVVMSWFHDPGATPMAVAGLLAKRQGDLGGGPSFFDVA
                     GRSQFVGTADRPDILLDAANQADNQLLNNSPSGFHNLLEGLGRINKVKVYGPLQPGLL
                     GTVEVTATTADHKSVARTVQVQLGAVSIPPVRAAVQVGQTLGTMQPGDASPVMVHWGD
                     LRALGDLVVQRVEDLALKSTAAPVTGQSYGEMLYAQDRWTEYWIGGTVSVIAPAPGQS
                     ANPPLSENVHLHQYPTPGVRLDQWDYDSIKKIALRWGTYYRLDHAGLLHPQGASDSDQ
                     GVRPADVLESQIVGNHRGLVFIDTVDGQPPRVDNNMGTL"
BASE COUNT          588 a          711 c          898 g          615 t
ORIGIN      
        1 catcgtattg gcgatcctgg gcatcatgag tatgttggct gggccgagtt atcacacagt
       61 ggttgcaagg gcccaggcgc gtagcgtcac cgcagagatc gcgtcggaac ttcggctcgc
      121 gagacagctg gcgatggcca ggcgagagcg tcttcgggtc atattcgacc gagagggccg
      181 taccatcacg cttcgacgcg ccgatgccga ggacatcctg cacgtctacc aatatgcaga
      241 caagggggtg gtcgtcgacg agccaacggc aggtcctgaa cttctgtttc atcccagtgg
      301 tcggtcggcg agccctacaa ccatttgcgt gcgggacagt cagggcaggg aaatcacatt
      361 taccgtaagc atcaccggaa gagtgtccgt ctcatgacgc gagatcgatt gtgtcgtgca
      421 gagggcggag ctgggtttgc tgaagtgttc gtggctatgg cgttgacgat gatcggattg
      481 gccggtgcaa tgggggcttt tcagactgct gaacggagtc ttcgtatggg catgttggcg
      541 acgagggctc tggcgatggc ggaatcgcgg atcgaagcaa aacgagctgc gagatgggat
      601 cgtctcttgc tagatgatct gaattacgat ggtatttcgg atcttgtgat gcatgacgat
      661 ggagcagggg gcgatgtgct ggcgggtgat gggatgtatt ccggtagttg ggatcaggac
      721 ggcgtccatc tgatctggac ggtgacgccg agccattctg gctcactgtc tgcctcaggg
      781 catgtcctca ttgatgcgcg cgcagtgtat gcagccgacg agggtccgca cgaggtacga
      841 attggtaccc tgcgggccaa tcccgtgttc gttggaggtc cgtaacgtgc catcactcgg
      901 agtggacgga atgcagacag gacagcggat gagtagcgcg tgccatagaa tgcggacaga
      961 ggcagggacc agcctgaccg agctgatgtg tgccatggca gctgggttgg tcgtcttggg
     1021 cgccacgctt caggccctgt cctattttca acatcgattt atgaagcaac aacatgaggt
     1081 cgcgcaacaa caagatcttc gccttgggtt agaagttctt gaagaggagt tgcggctggc
     1141 aggatccggt tctctcacca ttaccgcttc ggacacagtg gagttttcgg cgaatctgca
     1201 aggcctttcg acgacggtca ccgccgcgtc ggagatcggc cagacagctc tatcggtcga
     1261 agacgggcga gggtgggaag atcggaaaac cattgtggca tgctgggccg agcactgcga
     1321 gacacttgct ctggcacatg atggacaacg gcgcgtgctt acggtgacgc agccgctgac
     1381 acgtgtgatt ccatcaggag cctttgtctt cctcctgaat catgtccgct actacagccg
     1441 tctcgatgac caaggtgtgt tgaggctgct tcgacaagtc gatggcggcg cgagtgttat
     1501 ggtcagagat atccgagaag tgcggttctc ctactgggat gaaaacggac aggctgtgac
     1561 acaacccgcg ttcgtcaagt tggtcgttgt tgaggtgatg atgtccgacc aagggattcg
     1621 tgcggttcgg ggaatcagtc tcaggacata acggcggcat cttccaataa gcatggaagt
     1681 gaggataggg ggatgggaga acggggcgca tcaacattca tgaatcagtg cgacaggggt
     1741 cacgtgcttc tcgcggcgct catgctgatc tttctgctcg gggtcgtcgg catgacgtct
     1801 ctgtatctgg caggtcaaga tggaccgggc gtcagtgcca tgagagaaga caagcttgcg
     1861 caacagctgg ccgacggtgc caccgatgtg gtgatgagtt ggttccacga tccgggtgcc
     1921 acacctatgg cggttgctgg attgctcgcc aaacgtcaag gggaccttgg gggcgggccg
     1981 tcattcttcg acgtagccgg acgttcacag tttgtcggga ccgccgatcg tcctgacatc
     2041 ctccttgatg cggcgaacca ggcggataat cagctgctca acaattcgcc cagcggcttc
     2101 cacaacttgc tggaaggact tggccgaatc aacaaagtga aagtgtatgg tcctcttcag
     2161 ccaggattgc tgggtacggt tgaagtcacg gccacgacgg ccgaccacaa atcggtcgca
     2221 cgaacggttc aggttcagct tggggctgtg tcaattcccc cagttcgcgc ggcggtacaa
     2281 gttggacaga ctctcggcac catgcagcca ggcgatgcat ctcccgtcat ggtccattgg
     2341 ggtgatctac gggcgttggg cgatcttgta gttcagcggg tcgaagacct tgccttgaag
     2401 agcactgcgg ccccggtcac tggtcagtcg tatggcgaga tgctgtatgc acaagatcgc
     2461 tggacggaat attggatagg agggactgtt tcagtcatcg ctcctgcacc cggccaaagt
     2521 gcgaatcccc cactttctga aaacgtgcac ctgcatcaat atccgactcc aggagtgaga
     2581 ctcgaccagt gggattacga ctcgatcaag aaaatcgcac tacgctgggg gacctattat
     2641 cgactcgatc atgcgggact gcttcatccg cagggggcct cagattcgga tcagggagtc
     2701 aggcccgcag atgttctgga atctcaaatt gtcggaaacc atcgtggatt ggtcttcatc
     2761 gacaccgtcg atgggcaacc tccgcgtgtc gataataata tgggaacgct gg
//
LOCUS       DATIPF010000441         3702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1055346, whole genome shotgun sequence.
ACCESSION   DATIPF010000441 DATIPF010000000
VERSION     DATIPF010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055346"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..459)
                     /locus_tag="VLL06_10965"
     CDS             complement(16..459)
                     /locus_tag="VLL06_10965"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSS31528.1"
                     /translation="MQDSMRKSYSLMAILHEWKNERQVRRDAIDGHNLPPPSAASKGY
                     PMFPLRTLTEIDRLIMRVRSRLHEVSAPTVILQAREDDMTSPRNASIVYDEISSMVKQ
                     LVLLDDCYHVITIDKKREAVAENVAAFFQFQTVKILAHQEMQVGS"
     gene            complement(475..990)
                     /locus_tag="VLL06_10970"
     CDS             complement(475..990)
                     /locus_tag="VLL06_10970"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSS31529.1"
                     /translation="MMKPHRVTHAGLLDGMVDRQETDFALHTASNVLVYLIHGVTGTP
                     AEMSYLARKLARKNGWDVYVTTLPGHCTRLRDLVKTTEQDWRAHVQRQLSFIRERYEY
                     VFVAGLSAGALLALDASTVVRVDGIGVFSPTFVYDGWNTPWFNAILPLAMKVVPLSIQ
                     RFMFHIDGPPF"
     gene            complement(987..2378)
                     /locus_tag="VLL06_10975"
     CDS             complement(987..2378)
                     /locus_tag="VLL06_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSS31530.1"
                     /translation="MKPLPMIHVSEEALREKEAKYCSHGDTVHYSPVPKFFQGCEGSF
                     LYDREDKPYLDLQMWYSAVNFGYRNKTIIDALKAQLDRLPQLACQYLHEEKVLLSEKL
                     AKECMRAFGLEGRIQFNVGGAQAIEDSMKLVRNATGKSLFMAFTGGYHGRTLGASEIT
                     SSYRYRRRFGHFSSRAHFVPYPYCYRCPYGKKLDTCDYYCVNQVERLFETEYNSFWDT
                     KANESEFVAFYIEPVQATGGYIIPPPEYLSRLQKILNERKILLVDDEIQMGFFRTGKF
                     WAIENFGVNPNIVVFGKAMTNGLNPISGLWAQEALISPAKFPPGSTHSTFASNTLGTT
                     AALATLNWIEQQQYEKKVIESGAYFLERLETLKKRHATIGDVSGLGLALRIEVTKPDR
                     FTPDKAMADLIFETGLAGGLPSSRGPMGLVLDIGGHYKNVFTLAPSLDITKSEIDLGI
                     ELLEYLFTTCAKA"
     gene            complement(2375..3094)
                     /locus_tag="VLL06_10980"
     CDS             complement(2375..3094)
                     /locus_tag="VLL06_10980"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtnX-like HAD-IB family phosphatase"
                     /protein_id="HSS31531.1"
                     /translation="MQHNQATQATRRNKLRLRRCTTATEVFCDFDGTITRVDATDAVL
                     ETFALPAWRDWEQRWVNGEITSQVCLSRQIELIQADRDTLIRFADNLSIDEGIIALDR
                     RCAEEGVPLTIVSDGIDLLVEAVLRRHNLLHLPVYSNHLRWDKAGRPSLSFPFAVQEC
                     ESGAGTCKCALTRSSVLSPSRTVYIGDGRSDQCVSVKMQTLFAKGILRTWCEAQGITY
                     LPFETLTDVAEQIFSKETHIP"
BASE COUNT          892 a         1014 c          962 g          834 t
ORIGIN      
        1 gaaagtcatc cggagttaag agccgacttg catctcttgg tgggcgagga tcttcactgt
       61 ctgaaactga aaaaatgccg ccacattctc cgcaacggct tctcgttttt tatcaatggt
      121 aatgacgtgg taacagtcat ccagcaacac cagctgctta accattgacg agatctcgtc
      181 gtagacaatg gaagcattgc gggggctggt catgtcgtct tcccgcgcct ggagaatgac
      241 cgtcggtgca ctgacctcgt gcaatcggga ccgaacccgc ataatgagcc ggtcgatttc
      301 cgtgagcgtc ctcaagggaa acatcggata gcccttcgat gccgcagaag gaggaggcag
      361 gttgtggcca tcaatcgcgt cgcgccgcac ctgccgctca ttcttccatt catgcagaat
      421 ggccatgaga ctataggact tgcgcatcga atcctgcaac tgttcatcct tgattcaaaa
      481 cggaggccca tctatatgga acataaatcg ttggatcgaa agcgggacga ccttcatggc
      541 cagtggaaga atggcattga accacggggt attccaccca tcgtagacga acgtgggcga
      601 gaacactcca attccgtcta ccctgacgac cgtggacgca tccagggcca agagcgcccc
      661 cgcgctcagg ccagcgacaa agacgtactc atatcgctcg cggatgaagg aaagttgtcg
      721 ttgtacatgt gcgcgccaat cctgctcagt ggtctttacg agatccctca acctggtgca
      781 atgtccagga agggttgtca catagacatc ccatccattc ttcctggcca gcttccgagc
      841 aagatacgac atctcggctg gcgtgccggt gacaccatga atgagataga ccagcacgtt
      901 tgatgccgtg tgaagcgcga agtctgtctc ctgacggtcc accatgccat ccaagagccc
      961 cgcatgtgtg actcgatggg gtttcatcat gccttggcac acgtcgtaaa taagtactcc
     1021 agcagttcga tccccaagtc gatttcactc ttcgtaatat ctaacgacgg cgcgagcgtg
     1081 aagacatttt tatagtgtcc gccgatgtcc aagactaatc ccatggggcc gcgggacgaa
     1141 ggcagcccgc cggccaaccc cgtttcaaaa atcaggtccg ccatcgcttt atcaggagtg
     1201 aagcgatccg gtttcgtgac ttcgatacga agggctaagc ccaacccgga tacatctccg
     1261 attgtcgcat ggcgtttttt gagtgtttca agccgttcga gaaaatacgc acccgactcg
     1321 ataacctttt tctcatactg ttgctgttca atccaattaa gagtcgcaag cgcagccgtc
     1381 gttcccagcg tattggacgc aaaggtcgaa tgtgtggaac cgggaggaaa cttcgcgggc
     1441 gaaatgagcg cttcctgcgc ccataacccc gatatgggat tcaagccatt cgtcatggcc
     1501 ttcccgaaga ccacgatgtt cggattcacc ccaaagttct ctatggccca aaacttcccg
     1561 gtcctgaaaa aacccatctg aatttcatcg tccacgagca aaatcttccg ctcgttcaga
     1621 atcttttgca gccgtgagag gtactccggt gggggaataa tatatccgcc ggtggcctgc
     1681 accggctcga tataaaacgc gacgaactcc gactcattgg ccttcgtatc ccaaaacgag
     1741 ttgtactccg tttcaaacag ccgctcgacc tggttgacac agtagtagtc gcatgtgtcc
     1801 aattttttac cgtacgggca acggtaacag tagggatagg gcacgaagtg tgcccgactc
     1861 gagaaatgac cgaagcgacg gcggtaccga taactggaag taatctctga cgcaccaagc
     1921 gtccgtccat ggtagccgcc agtgaatgcc atgaataaac tcttgccggt ggcattgcga
     1981 acaagcttca tggaatcctc gatcgcctgc gcccctccca cgttgaactg aattcgccct
     2041 tccaggccaa atgcgcgcat gcattctttt gcgagcttct ccgacagcaa cactttttct
     2101 tcatggagat attggcaggc cagttggggc aatcgatcga gctgtgcttt gagggcatca
     2161 ataatcgttt tgttgcgata gccgaaatta accgccgagt accacatctg taggtcgaga
     2221 tacggcttgt cctcacggtc atagaggaaa ctgccctcgc agccttgaaa gaattttggc
     2281 accggcgaat agtgcacggt gtccccgtgc gaacaatatt tcgcctcctt ctcgcgaagt
     2341 gcttcttcgc tgacgtgaat catcggaaga ggtttcatgg aatatgagtc tccttggaaa
     2401 agatctgttc ggccacgtcc gtgagtgtct cgaacggaag ataggtaatc ccttgggcct
     2461 cgcaccatgt ccggagtatc cctttggcaa acagggtctg catcttaact gatacgcact
     2521 gatctgatcg gccgtctccg atgtagaccg tccgggaggg cgagagaacc gaggatcgcg
     2581 ttaatgcgca cttacaggtg ccggccccac tctcacactc ttgcacagca aaggggaagc
     2641 tgagcgaggg cctgccggcc ttatcccagc gaagatgatt cgaatagacc gggagatgta
     2701 agagattatg ccgacgtaag acggcttcta cgagcagatc gatcccgtca ctcacaatgg
     2761 tgagagggac gccttcttcc gcgcagcgtc gatcgagcgc aatgatcccc tcatcaatcg
     2821 aaagattgtc cgcaaatcgg atgagcgtgt cccgatccgc ttggatcagt tcgatttggc
     2881 gagagagaca tacttggctg gtgatctcgc cgttcaccca tcgctgttcc cagtcacgcc
     2941 aggccggcaa ggcgaatgtc tccagtaccg cgtcggtagc gtcgacccgg gtgatcgtcc
     3001 catcgaaatc acaaaacacc tcagtcgccg tggtacatcg gcgcagacgt agtttgtttc
     3061 gcctcgtggc ttgcgtggct tgattgtgtt gcaactgatc cttgtcgcta aagggaaccg
     3121 ccgtgcagag aacgagatct ccgaatcact cggcacatca caaaggtctc gtttccactg
     3181 cgaaattgaa aaactgaaac ggctaagcga tgggcggagg cctggagatg agcgaggcat
     3241 gaaaaagacg ctgagaagag agaggtcttt tcgtcggata cgcgatccga ctatagacat
     3301 cctgtggggt aagaccgtgt ccgactacag aatcattgtg aacaccgtca agaagcggag
     3361 tcacctcctc atacttcagt ccggttcccc ccccaacctt tgcattctct tctgcatcat
     3421 gggctggggc ttggtcgtcc aagtaaggaa cgtcagcaat gtacggcaga acgaagaaca
     3481 ccatcacgag ggccaggatg ctgcaccctg gccaacgatc gttttgatat tgtgacagtc
     3541 gaagcttcac agaatgtcac acgctgatgt cattaagaag gcttgcccat tactcagaca
     3601 gactgacatc gcagatcact tcggagggtg acgatgggaa tctcactgtc agagtactac
     3661 acgctcacca ccgaagcata tcacattttt gggtagatag tg
//
LOCUS       DATIPF010000442         5792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_173484, whole genome shotgun sequence.
ACCESSION   DATIPF010000442 DATIPF010000000
VERSION     DATIPF010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173484"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VLL06_10985"
     CDS             complement(<1..730)
                     /locus_tag="VLL06_10985"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSS31532.1"
                     /translation="MTKPQLFTAVFFALLLFLLYQIALMFRPFLFPIIWAALLAHLTF
                     PLHVRLTTFVRGNEIVSASCLTLLVLALVVIPISMMGVLLAREASTAEQTIREWVSSG
                     ALHTLPEQLSTWPVIGGLLQRLSSSVLLTPDSLEQGLLSAATFLSRFFIDQVGDLLKN
                     AFLLVTDFFLMLFALFFLFKDGKQWLASLQEVIPLDASHKQRIVDRMDQTIRAVVKGM
                     GLTAIVQGLAAGLAYGLLGVPFPVV"
     gene            complement(764..1219)
                     /locus_tag="VLL06_10990"
     CDS             complement(764..1219)
                     /locus_tag="VLL06_10990"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HSS31533.1"
                     /translation="MKTNSFAGDDNITLLAKGVLLRGEIHVEGTVRIDGRLDGDIQTK
                     GQVIIGEDGLVQGTITAGTVISSGRIKAKVTANERVQLMKTATLIGEVLTPILIIEEG
                     AKLQGVTDMGVTAWTDDLPKLSGSVSELSAHRAKQVVMLDKESSGDTRR"
     gene            complement(1282..3576)
                     /locus_tag="VLL06_10995"
     CDS             complement(1282..3576)
                     /locus_tag="VLL06_10995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HSS31534.1"
                     /translation="MKRSTGWVTYVSLVLAGIVAAFGLVVAIYGAYLATFLELPKSDD
                     HPPLRLYSGPFLLQPNLSLVQSRLLERLQRLGYRQVKVTQAPGDYQLTEEALTVYLHP
                     QPEGYVGATMVRLPLDQGRVMDVLSPLEGTPLFSIFLEPELLSGVRGESRQVREWIPL
                     AQIPPLIIETVLTIEDRRFYSHFGIDPIAVGRAIWTNFTKGGVVQGGSTITQQLAKNL
                     FYSPQRTMGRKFKEALAALVLEVKYTKQNILESYLNEIYLGQAGFVSIYGMSEAAHRY
                     FGKTLEQLTVDEVAMVAGLIKGPNSYAPTKHPELAKQRRDVVLRRLKEIGHLTEEAWN
                     RAVKEPVRVTPPEDVLTDAPYFVDAVLREVEEAGVVPIPEGSRIDSTLDPMIQQAATE
                     ALEKGLAKLEKTYSHLSNSPEPMQGAVVVLDPKNGSVLALVGGRDYRRSQFNRAIQAR
                     RQPGSLFKPFVYLAALEAAREGQVGQLTAASLVADEPVTFESGTGPWSPQNYDKQFHG
                     PITVRSALEQSLNVPAVQAAKTVGTKPIVQLLHRLGVTSAIGDDLSSVALGSSSVSLL
                     EITAAYGAIANGGIAVKPTAVRSTRDRQGDMVWNTIPNREQATSPQGAYVLTSLLEGV
                     IQRGTASKAKVLGLQGTVAGKTGTTDGYRDAWFVGYTSDVVIGVWVGFDDERSLRLTG
                     SQAALPIWMDLARRVIPPNVPAFVMPSGVVTRDIDPKTGQLATFQCPERVSEVFIEGT
                     EPSVYCEVHGGGIWERLRHTFGFS"
     gene            complement(3698..5101)
                     /locus_tag="VLL06_11000"
     CDS             complement(3698..5101)
                     /locus_tag="VLL06_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HSS31535.1"
                     /translation="MTLYLLLSVGVGLAAARRVHTAKDFAVAGRSLPLPIVIATVFAT
                     WFGAEAVLGISATFVKEGLRGVVADPFGSSLCLVLVGLFFAPRFYRLNLLTVGDFYRL
                     RYNRVVEVLCAVCIAASYLGWVAAQFKVFGLVLSTVTGGAVSQPVGMVIGAVIVLAYT
                     TFGGMFSVAILDFVQISVIIGGLLYIASVVSGLVGGVGVVIDHAASAGKLDFFPPASF
                     AAWIPFIGAWVTMMLGSIPQQDVFQRVTSAKDERTAVRGSILGGTLYFCFCFVPMFLA
                     YAATLVDPTLFNTLLAQDSQLVLPTLIMQHTPVLAQILFFGAVLSAVMSCASATLLAP
                     SVILSENVIKGALPRLSDGEFLRVMRMVVVVFAALVLTVALTSTSSIYHLVVNTYSVT
                     LVTAFVPLCAGLFWSRATTQGALCAFVAGLLTWIGLELFGPSDSVWHPQLLGLLMAAT
                     GMVVGSLLPQKIGTSNA"
     gene            5316..>5792
                     /locus_tag="VLL06_11005"
     CDS             5316..>5792
                     /locus_tag="VLL06_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31536.1"
                     /translation="MHDRIIHRLSICRKTGRLWLLAGVIGIGMLLAGLPAQASESQAP
                     WECSSYTGDAHTRCVGAFMELQRDQIAVLQEKMKAQEETVNRLKAQLDRQASTSSDLQ
                     RQLAQRPAVVQAAPPIYTYPSVGFGLYLGSPWIYGPPYYYPPFFYGPRYYGLGYWGH"
BASE COUNT         1366 a         1805 c         1544 g         1077 t
ORIGIN      
        1 gaacaactgg aaagggcact ccaaggagtc cataggccaa ccctgccgcc agaccttgca
       61 cgattgcagt caggcccatc cccttcacca cggcacgaat ggtctgatcc atccgatcga
      121 cgatccgctg tttatgggat gcatccagtg gaatcacttc ctgaagagac gccaaccatt
      181 gcttcccatc tttgaagaga aaaaataggg caaagagcat aagaaagaaa tccgtcacca
      241 gcagaaaggc gttcttcagc agatcgccga cctgatcgat gaagaaccgg ctcaggaacg
      301 tggcggcaga gagaagcccc tgttccagag aatcaggtgt cagtaacacg ctgctactca
      361 accgctgaag gagtcctccg atgaccggcc acgtgctcaa ttgctccgga agcgtatgga
      421 gtgctccgct cgagacccat tcgcgaatgg tctgctcggc cgtactggcc tcgcgtgcca
      481 gcaacacacc catcattgaa attggaatta ccacgagggc cagaaccagg agggtcagac
      541 aggaggccga cacaatctcg ttgccacgca cgaatgtcgt gagccgtaca tggagtggga
      601 atgtgagatg ggcaaggagc gcggcccaga tgatggggaa aagaaacggc cgaaacatca
      661 gggcgatttg atacaggaga aacagcagga gcgcgaagaa cacggcggta aagagctgcg
      721 gcttcgtcat ggattggaca ggaacaggag agaaccggca gagctatcgg cgcgtgtctc
      781 cgctggactc tttatcaagc atcacgacct gcttggctcg gtgcgcagag agctcactga
      841 cgctgccgga caacttcgga agatcgtcgg tccaagcggt cactcccatg tccgtcacac
      901 cctggagctt ggccccttct tctataatga gtatcggcgt caggacttct ccaatgagcg
      961 tcgcggtctt catcaactgg accctctcat tcgccgtcac cttggccttg atgcgcccac
     1021 tgctaatcac cgtaccggcg gtaatcgttc cctgcaccag tccatcttcg ccgatgatga
     1081 cttgcccctt ggtctgaatg tcgccatcga ggcggccatc gatccgcacg gtcccttcca
     1141 catggatctc gcctctcaac aacacgccct tggccaagag ggtgatgttg tcatctccag
     1201 caaagctatt ggtcttcata cgttattccc ccttctcacc cgatcgttga cgccagctta
     1261 cactattcct cccgacaagg actaggagaa tccaaaagta tgtctgagcc gttcccagat
     1321 tcctccccca tgcacttcac aatacacact gggctccgtg ccctcaataa acacttccga
     1381 tacccgctcg ggacattgaa acgtggccaa ctgtccggtc ttcggatcaa tgtcgcgggt
     1441 gacgacaccg gacggcatca cgaatgcagg aacattgggg ggaatgacac gacgcgcaag
     1501 atccatccaa atcggtaagg ccgcctgcga tcccgtgaga cgcagcgatc gttcatcgtc
     1561 gaatccgacc cacactccga tcaccacgtc agacgtatag ccgacaaacc aggcgtcgcg
     1621 atagccatcg gttgtgccgg tcttcccggc caccgtgcct tggagtccga gcactttcgc
     1681 tttgctcgcc gtgccccgct ggatgacacc ttcgaggagc gacgtcaaca cataggcgcc
     1741 ttgaggcgac gtggcttgtt cccggttcgg tatggtattc cataccatgt ctccctgccg
     1801 atccctggtc gatcgcaccg cagtcggctt caccgcaatc cctccgttgg caatcgctcc
     1861 atatgccgcc gtgatctcca gcaacgacac cgacgaactg cccaaggcca ccgacgaaag
     1921 atcatcgccg atggcacttg tcacgccaag acgatgcaac agctgcacga tcggcttggt
     1981 ccccacggtc ttggccgcct gaaccgctgg cacattgagc gactgttcca acgcgctccg
     2041 tacggtgatg ggcccgtgga attgtttgtc atagttctgc ggagaccagg ggccggtccc
     2101 cgactcaaac gtcaccggtt cgtctgccac caggctcgcc gcggtcaatt ggccgacctg
     2161 cccttcacga gccgcttcca gcgccgcaag atagacgaag ggtttgaaga gagagcccgg
     2221 ttgccgccgc gcctgaatgg cacggttaaa ttgacttcgt cggtaatccc gccctcccac
     2281 cagcgccagc acagagccgt tcttggggtc gagcaccacc accgcccctt gcatcggttc
     2341 cggcgaattg ctgagatggg aatatgtctt ctctaacttg gccaggccct tctccagcgc
     2401 ttcggtggcc gcctgctgga tcatcggatc gagcgtactg tcgatcctcg agccctccgg
     2461 tatgggaacc acgcctgcct cctccacctc tcgaagtacg gcatcgacga agtagggcgc
     2521 atcggtcagg acgtcttccg gcggagtcac ccgcaccggt tctttcacgg ctcgattcca
     2581 ggcctcttcc gtcagatgac cgatctcctt cagccgccgc aacaccacat cgcgccgctg
     2641 cttcgccagc tcaggatgct tcgtcggcgc ataactattc ggccccttga tcagcccggc
     2701 aaccattgcg acctcgtcga ccgtcaattg ctcaagagtc ttgccaaaat agcgatgggc
     2761 tgcctcgctc atcccataga tcgagacgaa tcccgcctgt ccgagataga tttcattcag
     2821 atagctttcc aggatattct gtttcgtata cttcacctcc agcaccaacg ccgccagggc
     2881 ctccttgaat ttccgtccca tcgtccgctg cggcgaataa aataaattct tcgccagttg
     2941 ctgcgtgatg gtgctgccgc cctgcaccac tccacccttg gtaaaatttg tccagatcgc
     3001 ccgccctacc gcaatcggat cgatgccgaa gtgggaatag aaccggcgat cctcgatggt
     3061 caggaccgtc tcaataatca gtggaggaat ctgtgccaac ggaatccatt cacgcacttg
     3121 gcgtgattca ccacgcacac cactcaggag ttccggttcc agaaagatgg aaaagagcgg
     3181 tgtgccttcc agcggagaca ggacatccat cacgcgaccc tggtccaacg gcaacctcac
     3241 catggtcgcg ccgacatagc cttccggctg cggatggaga tagaccgtca gcgcctcctc
     3301 ggtcagctga tagtcgccag gagcctgcgt caccttcact tgtcgatagc ccaaccgttg
     3361 cagccgttca agaagacggc tctgcacaag cgagagattg ggttgcagca ggaagggtcc
     3421 gctgtacaga cgtagagggg ggtgatcatc gctcttcggg agttcaagga aggtggccaa
     3481 atatgcgcca taaatcgcga cgacaaggcc gaacgcggcc acgatgccgg ccaggaccag
     3541 gctcacatat gttacccacc cggtagacct cttcataatg cgaggacctc accacagcat
     3601 aaccgatatg cggcaccggt agacggattc atctggttag tctagttcaa gcacatgcac
     3661 gaaacaaacc acgcacgcca gtgcagccaa ccggttttta tgcgttgctc gttccgatct
     3721 tctgaggcag aagtgaaccg acaaccatcc ctgtcgccgc catgagtaag ccgagcagtt
     3781 gtggatgcca gaccgaatcg gacgggccga acaattccag ccctatccag gtcagcaacc
     3841 cagccacaaa ggcacacaag gctccttgcg tcgtggctcg ggaccagaag agcccggcac
     3901 aaagtggaac gaacgccgtc acgagcgtca cactgtacgt gttcaccaca agatgataga
     3961 tgctggaggt ggaggtgagg gcgacggtca ggaccagggc ggcaaacacc accacaacca
     4021 ttcgcatgac ccgaagaaac tctccatcac tgagacgagg cagcgcccct ttgatcacgt
     4081 tttcgctcag tatcaccgat ggcgccaata gcgtcgcgct ggcacagctc atcaccgccg
     4141 agaggacggc tccgaaaaac agaatctgtg cgagtaccgg tgtatgctgc atgatcaacg
     4201 tcggcaacac cagttgagag tcctgagcga ggagcgtatt gaacagagtc ggatcgacca
     4261 gcgtggccgc ataggccaaa aacatcggca caaaacagaa acagaagtag agcgtgccac
     4321 ccaggatgga cccgcgcacg gcggttcgct catccttcgc cgaggtgacg cgctggaata
     4381 cgtcctgttg ggggatcgat cccaacatca tggtcaccca ggctccgata aagggaatcc
     4441 acgcggcgaa cgacgccggt ggaaagaaat ccaatttgcc ggccgaagcc gcgtggtcaa
     4501 tcacaacccc gacgccaccg accagcccac tgacgacaga tgcgatatag agcagccccc
     4561 caatgatcac ggagatctgg acaaaatcca ggatggcaac ggagaacatg ccaccgaagg
     4621 tcgtataggc caggacaatc accgccccga tcaccatccc gacgggctga ctcactgccc
     4681 ctcccgtcac cgtgctcaga acgagtccga acactttgaa ctgcgcagcc acccatccga
     4741 gataggacgc ggcgatgcat acagcacaga gcacctcgac gacacggttg taccgcagac
     4801 gataaaaatc gccgacggtc aggagattca ggcgatagaa acgaggggca aagaataacc
     4861 caaccaggac caagcagagg ctcgacccga agggatcggc gacgacgccg cgtaaccctt
     4921 ccttgacaaa ggtggcagaa attccgagaa cggcctctgc accaaaccac gtggcgaaga
     4981 ccgtggcgat cacgatcggc agcggcaggc tccgaccggc caccgcaaaa tccttcgccg
     5041 tatggacccg tctggcagcc gccagtccga cccccaccga caggagcaga tagagagtca
     5101 cgaatcccag aatcatgaaa gcggattggg catgatggga cggattctag cgaggcttcg
     5161 gcaagggcgc aatgattgag aggatggtga ttctagttca ttcggcaggt ctggtttacc
     5221 aaacacacga gacggactac ccgctcaagg ccaaccaacc ggccccctgt tacagtggaa
     5281 acttgcatgg taggctaata caaagaggga ggcccatgca cgatcgcata atccataggc
     5341 tctccatctg tcgaaagaca ggaaggctat ggctcctcgc cggagtgatc ggtatcggga
     5401 tgttgttggc cggattaccg gcccaggcga gcgagtcgca ggcaccatgg gaatgttcga
     5461 gttataccgg cgatgcccac acccgttgtg tgggggcctt tatggagttg caacgcgacc
     5521 agatcgcggt ccttcaagaa aaaatgaaag cccaagaaga gaccgtcaat cgcttaaaag
     5581 ctcagctcga ccgccaggcc tccacgagtt ccgacctaca acgccaactg gcccaacgac
     5641 ccgcggttgt gcaagcggcc cctcccatct atacctatcc ctctgtaggc ttcggcctct
     5701 atctgggaag cccctggatc tacggacctc catattatta ccctcccttc ttctacggcc
     5761 cgcgctacta tggactgggc tactggggcc ac
//
LOCUS       DATIPF010000443         5712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_83777, whole genome shotgun sequence.
ACCESSION   DATIPF010000443 DATIPF010000000
VERSION     DATIPF010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83777"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VLL06_11010"
     CDS             complement(<1..408)
                     /locus_tag="VLL06_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HSS31537.1"
                     /translation="MSERFHTLVELMAALRAPDGCPWDRKQTHESLKPYLLEETYEVL
                     DILDRRDRTKLPEELGDVLLQVLFHSQIASEAGSFTIEDVLEQLADKLIRRHPHVFKN
                     GSSDPTPTNADQVVMRWEDIKRTERQASGRPDSV"
     gene            884..1276
                     /locus_tag="VLL06_11015"
     CDS             884..1276
                     /locus_tag="VLL06_11015"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HSS31538.1"
                     /translation="MAAEQEEGFIVRDRRGGSHADRPSATSPAEPEIQPHHQAAHSDP
                     HQDSGIPVTFSSFVISLGSSSLMLMGEQLDPQQPPMPVNLPQAKEIIDLLSVLEEKTT
                     GNLTTEEQAVLRDMLYALRMKYVSLAAK"
     gene            1424..3766
                     /locus_tag="VLL06_11020"
     CDS             1424..3766
                     /locus_tag="VLL06_11020"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS31539.1"
                     /translation="MNHIKKDTGASPGIQNDRGSVTGGAPASAGAGRPLRPARPISEP
                     ERRTMHFRPVWIVLILLIPCLALTFYYSQVIIPGGEETDSFLPTTSYAFVLLLLNLDL
                     IGFVVLLLLLSRNLIKAYFERRHRLFGSGFRTKLVAAFIGFSLIPTVLLALVASGLVN
                     KAVDVWFSDQIEQVMRDSYDVARMQHAGHITLAVNSARAISHEIYREDMLLAEQRDLL
                     VAAMARKRAEFGVAGIEVFSAKMETLTKALDLEVPSSALDLPIGQLVLQVINGKQEVN
                     AVQEAQTGRLVRAAAPIAASGRAGEIGGVVVVEAYVPESLLAKMEGIGHQYDEYKQTK
                     AMKNPIKAGAYLFVAVITVLILFGATWFGFYVARSITVPIQRLAEATETIAQGDLSVR
                     IEAKATDEIGTLIDSFNRMTSDLQGSKTEIEEANISLRLSNLELDRRRAYIETVVETI
                     AAGLLSIDKNGRITNFNPSGERILGLTADRFRDRPANEAFKELKLDLFQTVYDRMLAD
                     QRDTLALEGQMEIDGRFLTIGLHGSRMKDEANKDLGIVLVFEDLTELIKAQKVAAWQE
                     VARRVAHEIKNPLTPIQLSAQRLRKKFFEKSPDFETIFDEATNVIVNEVTSLKHMVDE
                     FSKFARLPTPQMAHQSLHDVINEVTILYRGAHKDVELIVELDHDLPSLNFDREQLKRV
                     MVNLLDNAIQAMQQKGRVWLSTKYDTKRRMAVVSVADEGPGIAPEDQEKLFVPYFTRK
                     KTGTGLGLAIVRRIITDHEGQIHASQNQPKGAVFTFELPV"
     gene            3858..5252
                     /locus_tag="VLL06_11025"
     CDS             3858..5252
                     /locus_tag="VLL06_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSS31540.1"
                     /translation="MSASILIVDDEESILTSLSSILQDEGHEVTVVKNGIEALRVYTM
                     DPPDLMLLDIWMPEMDGMETLRRVRELVPTAQVMMMSGHGSIETAVKAIKLGAYDYIE
                     KPLSLENVTLRVKHALDQYRLEQENRTLRTTVQRKFELVGQSPAMLQLRQLIETAGPT
                     NSRVLIGGENGTGKELVARAIHQHSIRTGRPFVAVNCAAIPETLIESELFGHEKGSFT
                     GATSMKRGQFEQADGGTLFLDEIADMSLSTQAKVLRALQEQQFTRVGGTKLMKVDVRV
                     LAASNKNLLKEIEKGTFRDDLYYRLNVVPIIVPPLRERREDIPLLIRHFMKVHAEEQG
                     LRVKEVSPEAMAVFQQYDWPGNIRELRNLIERLMIMVPGPVIDPAHTGLSLQARPVGS
                     TSQPTTPAVNPLFTQSYDSLRDARNAFEKDYIARKLREHHWNISRTAEELKIERSHLH
                     RKIKLLDVEMRPEG"
BASE COUNT         1279 a         1519 c         1711 g         1203 t
ORIGIN      
        1 caccgagtct ggtcggccgg aagcctggcg ttccgtccgt ttgatatctt cccatcgcat
       61 gaccacctgg tcggcattgg tcggggtcgg atctgacgat ccatttttaa atacgtgggg
      121 atgtctccga atcagtttgt cggccagttg ctcaagcaca tcctcgatgg taaagctgcc
      181 ggcctctgac gcgatctgac tatgaaagag cacctgtaac aggacatcac ccagttcctc
      241 cgggagtttc gtgcggtccc gacggtccag gatgtccagc acctcatagg tttcttcgag
      301 aagatagggc ttcaatgact catgcgtctg cttacgatcc catgggcagc cgtccggcgc
      361 gcgcaacgcg gccatcaact cgacgagtgt atgaaatcgt tccgacatgg tggtcctcag
      421 ggtcacaggc agttgggcgc ccattatacc cggttcccga cagccttcaa ccggctagca
      481 ggctgtggaa aaactattgt ggcacagtga aactcaatta cccacgcgca tggtacaaca
      541 gaaggacccc acgcaggatg ctcaaaaagt cgcccttctc acccacccaa tcccagtgcg
      601 ccaagacgcg ccgttccgca ggcaaggccg caggcgagtc gaaaccggag acgtagcatt
      661 tctcacccgt ccaccccaag ctgccaagac agcttgttct ccgagtgggc tacgttgagg
      721 atttcgatga gccgagaatg aagcccggga aaaggcgcgt ctcggcgcgc tggggttggg
      781 cgggtgagaa gggcgacttt ttcagcgtcc tgttgtgcgt cttgcgcctc ttcagggcct
      841 atggtagggt accgtcactg catctggagc gggggaaagg cctgtggccg cagaacagga
      901 agaaggattt atcgttcggg atcggcgggg tgggagtcac gccgaccgtc cttccgccac
      961 gtcgcctgcc gagccggaga tacagcccca tcaccaggct gcacattctg atccgcacca
     1021 ggattcggga attcccgtca ccttttcatc cttcgtcatc tctctgggca gttcctcgct
     1081 gatgttgatg ggtgaacagc tggatcccca gcagcctccg atgccggtga atctaccgca
     1141 ggcgaaagaa attatcgact tgctgtcggt cttggaagag aagaccacag gcaatctcac
     1201 gacggaagaa caggcggtgc tgcgggatat gctctacgcc cttcggatga agtacgtgag
     1261 tttggctgcc aagtagtcgt accccctgcc gcctgtcgtc cccagacgtg tctgtagcca
     1321 tcaatggttc acacaacgtg gtggtgggac gcctgtctga ttcaatgaac gtgagtaggt
     1381 cgtctcccgt gtgccgaggc gccgacaatt ggggatgccg gatgtgaatc acatcaagaa
     1441 ggacacaggt gcctctcccg gaatacagaa tgaccgtggt tctgtcaccg gtggcgcacc
     1501 ggcatctgct ggagccggcc ggccgctccg gccagcccgt cctatttcgg agccggaacg
     1561 ccggacgatg cattttcggc cggtctggat cgtgctcatt cttctcatac cctgtctggc
     1621 gctgaccttc tactattccc aagtcatcat tcccggcggc gaggaaacgg attcctttct
     1681 gccaacaacc agctatgcgt ttgtcctcct cctgctcaac ctcgatctga ttgggtttgt
     1741 cgtcctgctg ctgctactct cccgcaacct catcaaagcc tacttcgagc ggcggcatcg
     1801 actattcgga tcgggatttc gaacgaagct ggtggcggcc ttcatcgggt tttcgctcat
     1861 tccaacggtg ctcctcgcat tggtcgcaag tgggctggtg aataaggcgg tggatgtgtg
     1921 gttcagcgat cagattgagc aggttatgcg ggattcatat gacgtagcac gaatgcaaca
     1981 tgccggtcac attaccctgg ccgtgaatag tgccagagcc atcagtcatg aaatttaccg
     2041 ggaagatatg ttgttggcgg aacagcgcga tctgctggtg gccgcgatgg cgcgcaagcg
     2101 ggctgagttc ggtgtggccg ggattgaagt cttctcggcg aagatggaaa ccctcacgaa
     2161 ggcactggat ctcgaggtgc cctcatcggc tctcgatttg ccgatcggcc agttggtgct
     2221 ccaagtgatc aacgggaagc aagaggtaaa tgcggtgcag gaagcccaaa ccgggcgttt
     2281 agtgcgggct gccgccccta ttgctgcgag cgggcgtgcc ggcgagattg gtggggtggt
     2341 cgtcgtcgaa gcctatgtgc ctgaatcgtt gctggcgaaa atggagggga tcggccacca
     2401 gtacgacgaa tataagcaga ccaaggccat gaagaatccc attaaggcag gcgcgtatct
     2461 cttcgtcgcc gtgatcacag tattgatcct gttcggggcg acctggtttg ggttttatgt
     2521 cgcgcggagt attaccgtgc caattcaacg gttggctgaa gcgacggaga ccattgccca
     2581 aggggatctc tccgtccgga tcgaggccaa ggccaccgat gaaatcggaa cactcatcga
     2641 ctcattcaac cgtatgacgt cggatctgca aggaagtaag accgagatcg aagaggccaa
     2701 tatctcgttg cgcctcagca atcttgaact tgatcgccgc cgtgcctaca tcgagacggt
     2761 ggtcgaaacg atcgctgcgg gcttgctctc gatcgataag aacgggcgga tcacgaactt
     2821 taacccctcc ggtgaacgga ttctcgggct tacggcggat cgtttccgag accgacctgc
     2881 caatgaggcg ttcaaagagt tgaaacttga tctgttccag acagtctatg atcggatgct
     2941 ggccgatcag cgcgataccc tggctctgga ggggcaaatg gagatcgacg gcagattttt
     3001 gacgatcggg cttcatgggt cgcgtatgaa ggacgaagcg aataaggatc tcggcatcgt
     3061 gctggtattc gaagatctca cggagttgat caaggcacaa aaagtggcgg cctggcagga
     3121 agtggcacgg cgggtggccc acgagatcaa gaacccgttg acgcccatcc aattgtctgc
     3181 gcagcgttta cggaaaaagt ttttcgaaaa gtcaccggat ttcgagacca tctttgatga
     3241 ggccaccaac gtgatcgtca acgaagtgac gagtctcaaa catatggtcg acgaattttc
     3301 gaagtttgcc cggctgccga ctccacagat ggcgcatcaa tcgctccatg atgtgatcaa
     3361 tgaagtcacg atcctatatc gaggggcaca taaggacgtg gagctgatcg tggaattgga
     3421 ccacgatctc ccatcgctca atttcgaccg ggagcagctc aagcgggtga tggtcaatct
     3481 gctggacaat gcgattcagg ccatgcaaca aaaagggaga gtgtggctgt cgaccaagta
     3541 tgataccaaa cgccggatgg ccgtcgtgag cgtggcggat gaaggcccgg gcatcgcacc
     3601 ggaagaccag gaaaaactat ttgtgccata tttcacgcga aagaagacag gaacggggct
     3661 gggattggcg atcgtgcggc gcatcattac cgatcatgag ggacaaattc acgcaagcca
     3721 gaatcagccg aaaggggccg tgttcacctt cgagttgccg gtctaatcac gtcagggata
     3781 cgcgttgagg agtgcggccg tggacgctgc tgctccgtaa cacctgacac tttatgtgta
     3841 acgcctaacg gagcgctatg tctgcatcga tcttaattgt ggatgacgaa gagtcgattc
     3901 tcacatcctt gagcagcatt ctgcaggatg aagggcatga ggtcacggtg gtcaagaacg
     3961 ggatagaggc gctgcgcgtc tataccatgg atccacccga tctgatgctg ctcgacatct
     4021 ggatgccgga aatggacggc atggaaacgt tgcgccgggt tcgtgagctg gtgccgacgg
     4081 cacaagtgat gatgatgtcg gggcatggct cgatcgagac ggcggtcaag gcgatcaagc
     4141 tgggggcgta tgactatatt gaaaaacccc tgtcgctcga gaatgtgacc ctgcgcgtca
     4201 aacatgcctt ggatcaatac cgtttggaac aagagaaccg gacgctccgg accacggttc
     4261 agcggaagtt cgagttggtg ggacagtcgc ctgcgatgct gcagttgcgg cagctgattg
     4321 aaactgccgg accgaccaat agccgggtgt tgatcggggg agagaacgga acaggaaagg
     4381 aacttgtcgc acgagcgatc catcagcaca gcatacgaac cggtcgaccg tttgtggcgg
     4441 tgaactgtgc ggccattccg gaaacgttga tcgagagtga gctgttcggg cacgaaaagg
     4501 gctctttcac cggtgcgacc tcgatgaaac gcgggcagtt cgagcaggcg gacgggggca
     4561 cactgtttct ggacgaaatc gccgacatga gtctgagtac ccaagccaaa gtcttgcgtg
     4621 cattacagga acagcaattc acccgtgttg ggggaaccaa gctcatgaag gtcgatgttc
     4681 gggttttagc cgcgtccaat aaaaatctgc tcaaggaaat cgagaaaggt acgtttcgcg
     4741 atgacctcta ctaccggctc aacgtggtgc cgatcatcgt gccgcccctc agggaacgcc
     4801 gggaagacat tccgttgctc atccgccact tcatgaaggt tcacgcagag gagcaggggt
     4861 tgcgagtgaa agaagtctcg ccggaggcca tggcggtgtt tcaacaatat gactggccgg
     4921 ggaatattcg ggaattgcgg aatctgatcg agcgattgat gatcatggta ccgggacccg
     4981 tcatcgatcc ggcgcacaca ggcctctcgt tgcaggcgcg cccggttgga tcgacgtccc
     5041 agcctacaac tcccgcagtc aatccactct tcacgcagtc gtatgactcg ctcagggacg
     5101 cccgcaatgc gtttgaaaag gactatattg cgcgaaagtt gcgggaacat cattggaaca
     5161 tttcccgcac ggccgaagag ttgaaaatcg aacggagtca tctccatcgg aaaatcaagc
     5221 tgctggatgt agagatgcgg ccggaaggat aggagggagc ggccaggtgc cctccttgct
     5281 cgcagaacgc gcacgatgaa actgtgctcg tccgatgcgc gcagtcgagg gcagccttgg
     5341 ccactccctt aaagtgatga tgtagagaga gaagggccct cgttggacgc gcgcaatcga
     5401 ggatcgacca ggctaccttc gaaaatgaaa tgaggaaatt ggaaagatgt gcacatggag
     5461 accatcccag ccgcctcgct gtaatggatg cgggtgaaaa gttggagcca tccctcgact
     5521 aggtctggtg cttgcgcgtt gacctccgca acagccctcc gctaagatgc gccctcctat
     5581 gacgacctat cgagatgccg gagtcgatat cgatgcgggc gacgagttcg tcgaccgtat
     5641 ttcaccactg gtgcggtcca cattccgtcc ggaagtcctg acggaccttg gcgggttcgg
     5701 cggattgttt cg
//
LOCUS       DATIPF010000444         5735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1412022, whole genome shotgun sequence.
ACCESSION   DATIPF010000444 DATIPF010000000
VERSION     DATIPF010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412022"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..3315)
                     /locus_tag="VLL06_11030"
     CDS             complement(85..3315)
                     /locus_tag="VLL06_11030"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSS31541.1"
                     /translation="MPGSSHASPKHDSHTPCILIALSLVLYSVPAWTQTQLAAIFGTI
                     SDSTGAVIAGAQVTVSSTNTGLNRVGLADTKGEYHVAGLPPGMYTVRAQKEHFQTQVI
                     EGIALNAGAAFPVNLSLRIGTVPQDVTVTADVAIDTTTSTVGGAITERSLTELPLNGR
                     DLFKTAVLQPGVAPTPSSAPSLLSNGQAGQVSVNGMRPSWTNVLIDGMDANDPVFGFS
                     PAGASGLFLGLNGLTEVRVLTQTFDAEYGTNGGGVIEAVTKSGSNQFHGSLWELHRDA
                     SLGARNYFDLPTRPIPAFVRNQFGASTGGPLVHDRTFFFANYEGFREVQASTVIATVP
                     DALAHQGLLPSAADPGACNNITPSGCVAVAIDPRLMQFLALLPPTNGADNGDGTGDLV
                     TASKGSTTENLGLIRVDHNFSNSHSLFARYMIDDSSSVVPSIGNPPGTYVPGFPALHE
                     ARNQYFTLQDRRSLGQEVFNELRFGINRTTASTSVIDTHPGLSISLVPGRPFGTIDVA
                     GMSLLGNSAVSPLGDFSTVYQVQDQLSFTRGRHTLKLGAQFRRIQSNGTLDFGVNGLY
                     TFQDLSPFGFQAHTNNPALEFFLQALPLSYVGVDPSNSDSNRDYRQNVVSGFAQDFFR
                     ATSRLSVNVGLRYDFYSNLTEADGRLSVIRNPATDSGPTVGKVFATTPRDLLSPQAGF
                     AWNIFGNGKTVLRGGAGVFRNQLPFFLFGIDRVLPPFFGITSYVFPSFLNPQNALLTQ
                     PIYVIQATYHPKFPYALQYNLNVEREIWPGTILTAGYFGARGNHLPREVEQNPFEPAL
                     GHRYNPNLPSPLLMVLTDAQSFYNSFQLSVSKHNSRNVSWQASYTFSHSVDDASSNAS
                     IEAVNEPPTTQDPFNRRGDRGRSGFDIRHNFVASVVYELPFARGRQFGGWQISAIANV
                     HSNVPFTPVLAFDNADVQSLLTSERPDLIGDPYKGVCPNGLRVGTPSCWFNPSAFTLP
                     APGQFGTAGRNILHGPAFAQFDLALDKGFKLKEGMKITFGAEVYNLLNRPNFAVPSNT
                     QSPLTPGGNGDAIFKDPAGHFASNVARIFSTVGTGRQIQLDARLTF"
     gene            complement(3445..4131)
                     /locus_tag="VLL06_11035"
     CDS             complement(3445..4131)
                     /locus_tag="VLL06_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS31542.1"
                     /translation="MSLVSTVDREAGSLSMAIAKPIVFVVDDDAWVRESLETLIRDEG
                     WQPETFATAQEFLDRPRAFTPSCLVLDISLPGLNGLELQKRVAERTDMPIIFITGHGD
                     IPMSVGAMKAGAVEFLTKPFNDRILLMAIRQALERSCVALARETEMQELRDHYASLTP
                     RERDVMKLVVSGLLNKQVAGELGIGESTVKAHRGQVMQKMKANSVADLVKMTARLYPS
                     AATNRSMSSR"
     gene            complement(4165..4533)
                     /locus_tag="VLL06_11040"
     CDS             complement(4165..4533)
                     /locus_tag="VLL06_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS31543.1"
                     /translation="MVRSLLSVVDDDEMLRESLPDLLREVGFEARAFSSGQEFLSSQY
                     VDETSCLILDVAMPGMSGLDVQQELKRRGQAIPIIFITGQKDEDVREKAFRQGAVKFL
                     YKPFSDNALLDAVNEVLSKV"
     gene            complement(4635..>5735)
                     /locus_tag="VLL06_11045"
     CDS             complement(4635..>5735)
                     /locus_tag="VLL06_11045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS31544.1"
                     /translation="SKTGSTITDLLADVHIWSEEMFRICEFDPTTKITVKTFREIIHP
                     DDRPLFDALVARGMTGEDLYFLYRIVTPQGVVKHVRGIARVTEHVAGRPLFIGALQDV
                     TESKLAEECLDRARSELAHVARVTTLNALTASIAHEINQPLASLITNASICLRRLNAD
                     PPNIDGARETLMRTIRDGNRAGDVIARLRALYSKKAFTPELLDLNEATREVIALSLSD
                     LQRKSVILRSELADGLPLVTGDRIQLQQVILNLVRNASDAMGSVDDRPRELLIRTEQE
                     GADRVRLSVKDAGLGFTGEAADKLFQAFYTTKTDGMGIGLHVSQSIIEAHHGRLWAIA
                     NDGPGATFSFALPCRVEGSEDIQTRSDRGDET"
BASE COUNT         1358 a         1549 c         1567 g         1261 t
ORIGIN      
        1 aaagcctctt gacgataatc ctgagtgcga agactgtctg cgcgacaaga tcttttgcgg
       61 tctcagaatt cttggggtgg tctctcagaa agtcaaccgt gcatccagtt gaatttgacg
      121 ccccgtaccc acggtagaga atattcgggc gacattgctg gcgaaatgtc ctgcagggtc
      181 tttaaagatg gcgtctccat ttcctcccgg ggtgagtgga ctctgcgtgt tgcttgggac
      241 tgcaaaattt ggacgattca gtaaattgta gacttccgct ccaaaagtaa tcttcatgcc
      301 ttccttcaat ttgaaaccct tatcgagtgc cagatcaaat tgtgcgaagg ccggcccgtg
      361 caggatgttg cgtccagcgg taccaaactg accaggagct ggcaacgtaa acgcactcgg
      421 attgaaccag catgacgggg tgcccactct aaggccgttt ggacacacac ctttgtaagg
      481 atcgccgatg aggtctggcc gttccgaagt caatagtgat tgcacatctg cattatcaaa
      541 ggcaagcact ggagtaaaag ggacgttgct gtggacgttg gcaattgctg aaatctgcca
      601 gccgccgaat tgccttccgc gcgcaaaagg cagctcgtaa acaacgctcg caacgaaatt
      661 gtgccgaatg tcgaatcctg accgcccacg atcccccctg cgattgaacg ggtcctgagt
      721 cgtgggcggt tcgttgaccg cttctatgga agcattgctt gaagcatcat ctaccgaatg
      781 agaaaacgtg taggacgcct gccaggaaac attacgggag ttatgttttg aaacggagag
      841 ttggaatgaa ttatagaaag actgcgcatc ggttagcacc attaacagag gcgaaggcaa
      901 gtttggattg tagcgatggc ccaacgctgg ttcgaaaggg ttctgttcta cttcgcgagg
      961 gaggtggttg ccgcgcgcac caaaataccc ggcggtcagg atcgtacccg gccagatttc
     1021 ccgttccacg ttgagattgt attgcaacgc gtaagggaac ttcggatggt aagtcgcctg
     1081 aataacgtat atgggttggg ttagcaaagc gttctgcggg ttgagaaagc tagggaacac
     1141 ataggaagtt atgccgaaaa aaggaggcag aacccgatcg ataccaaaca ggaaaaaagg
     1201 gagctgattg cgaaagacgc cggctccgcc ccgcagtacg gtcttgccgt taccgaagat
     1261 gttccatgca aagccagcct ggggtgacaa cagatcgcga ggtgtcgtag cgaacacctt
     1321 gcccactgtg gggccggagt ccgtggcagg attgcgaata accgaaagac ggccgtctgc
     1381 ctcggttaga ttggaataga agtcgtaccg taacccaaca ttcacggata aacggctagt
     1441 tgctcgaaag aaatcctgcg caaacccaga cactacgttc tggcggtaat cgcggttgga
     1501 atcggagttg gagggatcaa cgcccacgta cgacagtggc agagcttgaa ggaagaactc
     1561 gagagcaggg ttgttggtgt gcgcctgaaa cccgaacgga ctcaaatcct ggaacgtata
     1621 cagcccattc acgccaaaat ctagtgtgcc attggattgg atccttcgaa actgcgctcc
     1681 gagtttcaag gtatggcgac ccctagtaaa tgacagttgg tcctgaacct gataaacagt
     1741 cgaaaaatcg cccaacgggc ttactgcgct attgccgagc agactcattc cggcaacatc
     1801 gatcgtcccg aagggtcttc caggaaccag agaaatcgaa agccctggat gcgtatcaat
     1861 gactgaagta gatgctgtcg tccggttaat accgaatcgt aattcgttga acacctcctg
     1921 tccgagactc ctgcgatcct gaagagtaaa gtactgattg cgggcttcat gaagagccgg
     1981 gaacccgggg acgtaagttc ctggtgggtt tccgatagaa ggaactacgg acgaactgtc
     2041 atcgatcatg taacgggcga atagtgaatg tgagttagag aagttgtggt cgacacggat
     2101 aaggccaaga ttctcagtag tagaaccctt actggcggtg acaaggtcgc cggttccgtc
     2161 tccattgtcc gccccattcg tcggaggaag cagcgcgaga aactgcatta agcgaggatc
     2221 gatggcaacg gcaacgcacc cgctgggagt gatgttattg caggctcccg gatcagcggc
     2281 agagggcaac aagccttggt gagccagagc gtcgggcaca gtcgcaatga ctgtggatgc
     2341 ctgcacttct cggaaaccct cgtagtttgc gaagaagaag gtgcgatcat gcaccaacgg
     2401 ccctccggtg ctggcgccga actggttgcg cacgaatgct gggatggggc gggtaggcaa
     2461 atcaaaatag ttcctggcgc cgagtgaagc atctcggtgg agttcccaca gcgatccatg
     2521 gaattgattg cttcctgact tggttaccgc ctcgatgacc ccgcctccat tggtgccgta
     2581 ctccgcatcg aaggtttggg tcagaacgcg tacttcggtc aatccattca gaccgagaaa
     2641 aagccctgaa gcgcccgccg gcgagaagcc gaagacagga tcattcgcgt ccattccgtc
     2701 gatgagaaca ttcgtccagc tagggcgcat accgttaact gaaacctggc cggcctgccc
     2761 attcgacaag agcgaaggag cactgctggg ggtcggggcg actcccggtt ggaggacagc
     2821 ggttttgaat aggtcgcggc cgttcagcgg aagttcggtc aggcttcgtt ctgtaatcgc
     2881 accaccgacg gtcgacgtcg tggtgtcgat cgccacgtcc gcagtgaccg tcacgtcttg
     2941 tggaactgtg ccaattctca gcgacagatt gaccgggaat gcggcacctg cattaagagc
     3001 aatcccttcg atcacctgtg tctgaaagtg ttctttctgt gctcgaaccg tatacattcc
     3061 aggcggcaag cccgccacat gatactcacc cttggtgtcg gcaagaccaa cgcggttcaa
     3121 tcctgtattt gtgctagaga ccgtgacttg tgctcccgca atgacagcgc ccgttgaatc
     3181 agagatggta ccgaaaatcg ccgcaagctg cgtttgcgtc catgcgggta ctgaatataa
     3241 gaccagcgag agagctatga gtatgcacgg agtgtgcgaa tcgtgtttag gcgaggcatg
     3301 agagctaccg ggcatgactg ctaccgcctt gtctattctg aaaaagggca catagcccac
     3361 ccccaaccga taatcgcgca actgctgaga attcggtggc gcggcgtcaa aacctcaatc
     3421 gccgtcgggt aagtcccaac ttcgctagcg agaactcatt gaacggtttg tggcagcact
     3481 tggatatagt ctcgcggtca ttttcaccaa atctgccaca gagttggcct tcatcttctg
     3541 catcacctgg ccgcggtgtg ccttcactgt gctctcgcca atgccgagct cgccagcgac
     3601 ctgtttgttc aacagaccgg agacgaccaa cttcataacg tccctttcgc gaggcgtaag
     3661 tgatgcgtaa tggtctcgga gttcctgcat ctctgtctcc cgagccaatg ctacgcagct
     3721 tcgttctagg gcttgtcgga tcgccatcag caatattctg tcgttgaagg gcttcgtcag
     3781 gaactcgacc gccccggcct tcatggcgcc gactgacatt ggaatgtcgc cgtgtcccgt
     3841 gatgaagatg atcggcatgt cggtgcgttc cgcgacgcgc ttttgcagtt cgaggccatt
     3901 gagtccgggg agggaaatgt cgagcacgag acaacttgga gtaaatgccc gcgggcgatc
     3961 gaggaattct tgtgcggtgg cgaacgtctc tggctgccag ccttcgtctc gtatcaaagt
     4021 ttccagcgat tcacgcaccc atgcgtcgtc atcaacaacg aacacgatgg gcttggcaat
     4081 tgccattgat agtgaccccg cttcacgatc aaccgtgctg actaaactca ttgtagccct
     4141 cggaacttgc atacagctgg gcagtcatac cttagaaagg acctcgttga cagcatcgag
     4201 caaagcattg tcgctgaacg gcttgtatag gaacttcaca gctccttgtc tgaacgcttt
     4261 ttcccggacg tcttcatcct tctgaccggt gatgaaaatg attggaatag cttgtccacg
     4321 acgtttcagt tcctgttgaa catcgagtcc ggacattccc ggcatcgcga catcgaggat
     4381 gagacaacta gtctcatcca cgtactgaga cgagagaaac tcctgcccag acgaaaaagc
     4441 ccgggcctcg aacccaactt ccctgagcag gtcgggaagc gactcgcgta gcatctcatc
     4501 gtcatcaacc acggataaaa gcgaacgtac catggcggtc tttccctgca taacaacaca
     4561 atgcgtcaat ccaccagccg cgtctattcg actttagtac tactttcgcc caaagagaac
     4621 caagcggagt ccaatcacgt ctcatctccc cggtcggaac gagtctgtat atcctccgag
     4681 ccctccactc tgcaagggag agcgaatgaa aatgtggctc cgggcccatc attggctatc
     4741 gcccacagac gtccatgatg agcctcaatg atggactggc tgacatgcag cccgattccc
     4801 attccgtcgg tcttggttgt ataaaatgct tgaaatagct tgtccgcggc ttcacctgta
     4861 aagccgagcc ccgcatcttt cacgctaagg cgtacgcggt ctgcaccttc ctgttcggtt
     4921 ctgatgagca actccctcgg gcgatcatcc accgagccca tggcatccga ggcattgcgg
     4981 accagattaa gaatcacctg ctgcaactgg atacgatccc ccgtcaccag cggaaggcca
     5041 tcggcgagtt ccgatctcag aatcacgctt ttcctctgaa gatcgctcaa cgacagtgca
     5101 atcacttccc gggttgcctc attcaaatcc agcagctccg gcgtaaatgc ctttttgcta
     5161 tacagggcgc gcaatcgcgc gatcacatcc ccagcgcggt tgccatcgcg aatggtacgc
     5221 attaacgttt cacgcgcacc atcaatattc ggaggatcag cattcaacct ccgaagacat
     5281 atactggcgt tggtgatgag gctcgcgagg ggctggttga tctcgtgcgc gatggaggcg
     5341 gtcaaagcat ttagcgtcgt gacacgcgcc acgtgggcaa gctcagagcg tgccctgtcc
     5401 aagcattcct cggcaagctt gctttctgtc acgtcctgta aagcaccgat gaacagcggc
     5461 cggcccgcga catgctccgt gacacgcgcg atgccgcgaa cgtgcttcac aacaccttga
     5521 ggggtcacaa tccggtatag aaagtaaaga tcctcacccg tcatgccgcg cgcgactaaa
     5581 gcatcgaaca acggccggtc atcggggtgg atgatttccc gaaacgtctt cactgtaatc
     5641 ttggtcgtgg gatcgaattc gcagatgcgg aacatctcct cggaccagat gtggacgtcc
     5701 gccagcaaat ccgtgatggt gctgcctgtc ttgct
//
LOCUS       DATIPF010000445         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_351972, whole genome shotgun sequence.
ACCESSION   DATIPF010000445 DATIPF010000000
VERSION     DATIPF010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351972"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="VLL06_11050"
     CDS             <1..1159
                     /locus_tag="VLL06_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HSS31545.1"
                     /translation="LQRDALGRTVFPKSLAEQVPTPGHALTLTVDEVIQYIAEKELEE
                     AVTHSRAKSGTIIVMEPHSGAILAMAVSPRFDPNVVAALTPDRWRNRALTDTYEPGST
                     MKIVVAAAALEEKVMTPGSMIFGENGQMMIANTTIHDHEKLGWMTFSEVIQKSSNIGA
                     AKTGMALGEQRLYRYLQSFGFGQRTEIDLPGEVNGLLKVPKDWGRRSLASISMGQEVG
                     VTPLQMVSAVATLANGGVMMKPYVVSEVRDQNGRLLMEVRPQVKRRVVSPDTARMMTT
                     MLEGVVTNGTGTKGAIPGFRVAGKTGTAQKIDPRTGTYSSTQFVGSFVGYVPAESPRL
                     AMIVVLDEPQGEAWGGTVAAPVFRRVGEQVLNYLGVSREDTVKIALAMKED"
     gene            1166..>2425
                     /locus_tag="VLL06_11055"
     CDS             1166..>2425
                     /locus_tag="VLL06_11055"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HSS31546.1"
                     /translation="MVRDTITLTQLLAALRGQVEVLEQRGDASRPVHAITDDSRAVTG
                     GSLFVAVKGERVDGHGFVEQAVKAGAVGVVAQASVASGSLPFVRVTDSRKALGLLGGR
                     FYGDPSNRLKMIGVTGTNGKTTTTYLCKALLERIGQRVGLIGTVAYQIGQETIPASHT
                     TPGALDLQALLSKMVESGLTTAVMEVSSHALALDRTAGCEYDVAIFTNLTQDHLDFHH
                     TMDGYFEAKLRLFTGLAGGQKAGKLALVNLDDPYGAAIRAACPVPVWGYALKKQADFK
                     AERVHLSLTGTTFTAATPIGSFTVESQLVGEHNVYNLLGAIGVAVHEGATSNQICEAT
                     AHITNVPGRFERVSSEQEFTVVVDYAHTEDALLRLLTAAQTLKTGRIITVFGCGGDRD
                     RGKRPKMGRAAVEYSDVVVLTSDNPRTE"
BASE COUNT          516 a          619 c          780 g          510 t
ORIGIN      
        1 attgcagcgc gatgccttgg gccgtacggt ttttcccaag agtttggcag agcaagtgcc
       61 gacgccggga catgccctca cgctgacggt cgacgaggtc atccaataca ttgcagaaaa
      121 agaactggaa gaggcggtca ctcattcccg tgccaagtcc gggacgatca tcgttatgga
      181 gccgcattcg ggtgcgattc ttgcgatggc tgtcagtccg cgattcgatc caaatgttgt
      241 cgctgcgttg acgcccgatc ggtggcgtaa tcgtgcgctg acggatacgt acgagcctgg
      301 gtcgaccatg aaaattgtgg tcgcggcggc ggcgcttgaa gaaaaagtga tgacgcccgg
      361 ttccatgatc ttcggtgaaa atggccaaat gatgattgcg aatacgacga ttcacgacca
      421 tgaaaaattg ggatggatga cgttctccga ggtgattcaa aaatccagta acatcggcgc
      481 cgccaagacc ggaatggctc tcggagaaca gcggctctac cgctatctcc agtcgttcgg
      541 attcggccaa cgtacagaaa tcgatctacc gggtgaagtg aacgggctct tgaaggttcc
      601 caaagactgg ggccgccgat cactggcatc gatttcaatg ggacaggagg tcggggtgac
      661 gcccctccag atggtgtcgg cggtggcgac gctcgccaat gggggcgtga tgatgaagcc
      721 ctatgtggtg tctgaggttc gcgatcagaa cggtcgcttg cttatggaag tacggcctca
      781 agtcaagcgg cgggtcgtgt caccggatac ggccaggatg atgaccacga tgttggaagg
      841 tgtcgtgacg aatgggacag gaacgaaggg ggctattcct ggattccgcg tggcgggcaa
      901 aactgggacc gcgcaaaaaa tcgatccgcg gaccggtacc tattcctcca cccagtttgt
      961 cggatcattt gtcgggtatg tgccggcaga gtctccgcga ttggcgatga tcgtggtcct
     1021 cgatgaacct caaggagagg cctggggtgg gaccgtggca gcacccgtct ttcggcgggt
     1081 cggcgagcag gtcctcaatt atctcggagt gtccagggag gacacggtga aaatcgcact
     1141 ggcgatgaag gaagactaag agcccatggt gcgcgatacc attactctca cgcaattgct
     1201 cgcggcgctc cgtgggcaag tcgaggttct ggagcagcga ggcgatgcga gtcgtccagt
     1261 gcatgcgatc acggacgatt cgcgtgccgt aaccggcggg agcctttttg tcgccgtcaa
     1321 gggtgagcgc gttgatggtc atggatttgt cgagcaggcc gtcaaggcgg gagcggttgg
     1381 ggtggtcgcg caggcttctg tggcatcggg gtcgttaccg tttgtacggg tgaccgactc
     1441 gcgcaaggcg ctcggtctcc tcggaggccg gttctatgga gatccatcca atcgcctcaa
     1501 gatgattggt gtgacgggta caaatggaaa gacgacaacg acatatctct gcaaagcgtt
     1561 actggaaaga atcggccagc gggtggggct gatcggcaca gtcgcgtacc agattgggca
     1621 ggagacaatc ccagcctctc acacgacacc cggtgcactc gaccttcagg cactcttgtc
     1681 gaagatggtc gaaagcggac tgaccaccgc ggtgatggaa gtgtcgtccc acgccctcgc
     1741 attagaccgg accgcgggct gcgagtatga cgtggcgatc ttcacgaacc tcacgcagga
     1801 tcatctcgat tttcatcata cgatggatgg gtactttgag gcgaagttgc gactgtttac
     1861 cggattggcc ggagggcaga aggccgggaa acttgccctg gtcaatctgg acgatcccta
     1921 tggagccgca atccgcgcag cctgtcctgt accggtctgg ggttatgccc ttaagaagca
     1981 ggccgatttc aaagcggaac gagtacacct ctcgctgacc gggaccacgt ttactgcggc
     2041 aactcctatt ggatctttta ccgtcgagag ccaattggtc ggggagcata acgtttacaa
     2101 cttgctgggg gcgattgggg tggcggtgca cgagggggcg acgtccaatc agatctgcga
     2161 agcgacggct catattacca atgttcctgg tcgtttcgag cgggtctcct ccgagcaaga
     2221 atttacggtc gtcgttgatt atgcccatac ggaagatgcg ctgctccggt tgctgacggc
     2281 agcccaaacg ttgaagaccg ggcgcatcat taccgtattt ggttgcggcg gtgatcggga
     2341 tcgaggaaag cggcccaaga tgggtcgtgc tgccgtggaa tacagtgatg tcgtggtctt
     2401 gacgtccgat aaccctcgga ccgaa
//
LOCUS       DATIPF010000446         3419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1678849, whole genome shotgun sequence.
ACCESSION   DATIPF010000446 DATIPF010000000
VERSION     DATIPF010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678849"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VLL06_11060"
     CDS             complement(<1..311)
                     /locus_tag="VLL06_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HSS31547.1"
                     /translation="MDRFRTALQLYKDQNGHPGTRPHAGKAASGQALPPPIVYTRTRS
                     LDIPLSMLRQQRVMAAYDKGPFVDAIKILRTQVLQRLRENNWNVLGVTSPGHAEGKTL
                     TA"
     gene            complement(357..2120)
                     /locus_tag="VLL06_11065"
     CDS             complement(357..2120)
                     /locus_tag="VLL06_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31548.1"
                     /translation="MTTPHPSRHSEPIQGLQDYLAIFRRRRMLTFTTGGVLLILSLTA
                     AFLWPPTYKSMATILIEEQEIPSELVHSTITSYADQRIETIKQQVMSRTTLWKVVEQY
                     NLYADLRRTSPAEEVVKRFIKDIGVDVISADVVDKRTQHPTKATIAFTVAYQSNSPDL
                     AQKVANELTSLFLGQNLKSRERQAQEATSFLQQEADNLSTHISEVDGKIAEFKHRASG
                     ALPELMPLNLQLMSQSDRELTEIDQQIRTVEERTNYLEGELATIKPNTPIMSVTGERI
                     LDSSERLRALHAEYTGASANLSSDHPDIQKMKQEIEALEKETGQHPEVEEASKRLIDA
                     RATLAAALKRLGSEHPDVIQARRTINALEREVRQLRSMSPQKSMLRPENPAYINLQAQ
                     LNSATSSLSALRATRVAVKQRLEDYAARLQRTPEMEPDYLFLTRDRDTSGQKYQEIRS
                     RLLEAKVSEGLEVQRKGERFSLIDPPSLPEKPDKPNRSAIVLLGFILAIAGGIGTAAT
                     AESLDHSIRTPEQLVHLTQMFPLAVIPFMPNEEDLSRAMKRRLLLQGAGVGTFAAILA
                     LVHVFVMPIDVLWFTALRRFG"
     gene            complement(2132..3271)
                     /locus_tag="VLL06_11070"
     CDS             complement(2132..3271)
                     /locus_tag="VLL06_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31549.1"
                     /translation="MASLSEAAEWSLLPSIGVKGLYNSNLLLTPLPHDETYGYWTSPA
                     AEFAGKTERLEVSGRIAADFVSYYGGQQNNFTNIHGPVTIHYKTDKDVLGFTGGFNRD
                     NTLMSELLTTGLVLSFTQRNQWTANPSWSRRITEKLSFQSSGQLSDTTYENGLHLGLV
                     DYQLFGGSGGLLYQMTEQTQIQLSGSYVNFHTINAPSPFRASFPGINLNLTHAFTETL
                     TGTIYGGPRFVSSTTQTISENVKAQDTVWLFGASLDKKFERTSVQLNVDRDIVPSGFG
                     LLIQRDRAGLSVSHDLTETLAASVNGSGYLISGVTNRAQGGTFPDSRYFYLTPKIAWK
                     FLDWWKAELSYTYGWRDVDGFSDPAMSNATMFMLTYYPPKLAFSN"
BASE COUNT          776 a          885 c          921 g          837 t
ORIGIN      
        1 gcagtcaacg tcttgccttc agcgtggcct gggctggtga ctcccaatac attccagtta
       61 ttttctcgaa gccgttgcag gacttgggtg cgcaaaatct taatggcatc gacgaagggg
      121 cccttatcgt atgcagccat cacccgctgc tgacgaagca tcgagagtgg aatgtccagt
      181 gatcgtgttc tggtatagac gatgggagga ggcagggcct ggccactggc agcctttccg
      241 gcatgcggcc tggtcccagg gtgaccattt tggtctttat acagttgcaa cgcagtccga
      301 aatcgatcca tggattctcc tatgtatctc gaacattcat gctgccatat gccaacctag
      361 ccaaatcgcc tcaaggcggt aaaccacagc acatctatcg gcatcacaaa cacatggacc
      421 aaggcgagga tcgccgcgaa tgtgccaact ccagctccct gaaggagaag acgtcgcttc
      481 attgcccggg agagatcctc ttcattcggc ataaaaggga tcacggccag cggaaacatc
      541 tgagtcaaat gcacaagttg ttccggtgtg cgtatcgaat gatcgagtga ttccgcggtg
      601 gcggcagttc cgattccacc agcgatggcc aaaataaagc cgagaagcac aatcgcagac
      661 cggtttggct tatcaggctt ctccggcagg ctaggaggat cgatgagcga aaaccgttca
      721 ccttttcgtt gaacttctaa cccttccgaa accttggctt caagcagtct ggaccgaatc
      781 tcctgatatt tctgccctga ggtgtcgcga tcgcgcgtca gaaaaagata atccggctcc
      841 atctcaggag tccgttggag gcgggccgca tagtcctcaa gccgctgttt cacagccacg
      901 cgagtcgccc gtagtgcact taaggaagac gttgcggaat tgagttgagc ctggagattg
      961 atgtaggcgg ggttttccgg cctcagcatg gacttttgag gagacatcga gcgaagctgg
     1021 cgaacctccc gttccagggc attgatcgtg cgacgagcct gaatgacatc tgggtgttca
     1081 ctgcctagac gtttcaaggc agcagccagc gttgcacggg cgtcgatgag tcgttttgac
     1141 gcttcctcga cctcgggatg ctgtccggtt tccttttcca aggcctcgat ctcctgtttc
     1201 atcttctgta tatctgggtg gtcggaagac agattggcag aggcgccggt atactccgca
     1261 tgcaaggcgc gtaagcgctc ggatgaatcc aggatgcgtt ctccagtcac ggacatgata
     1321 ggagtattgg gtttgatcgt cgcaagttca ccttcaaggt agttcgtgcg ttcttcgacg
     1381 gttcgaatct gctggtcgat ctccgtcagt tctcgatcag actgactcat gagctgcaaa
     1441 ttcaggggca tcagctccgg caacgcaccg cttgcccggt gcttgaactc ggcaattttt
     1501 ccatccacct cgctaatatg cgtggaaaga ttatcagcct cctgttggag gaacgacgtc
     1561 gcctcctgcg cttgccgttc tcgactcttt agattttgac ccaggaagag gctggttagc
     1621 tcgttcgcga ccttttgagc caaatcaggc gagttgctct ggtatgccac ggtaaatgca
     1681 atcgtggctt tcgtgggatg ttgagtgcgc ttgtccacga cgtctgcgct gatcacatca
     1741 actccgatat ctttgataaa tcgttttacg acttcctctg cggggctggt tcgacgcagg
     1801 tctgcataga gattgtattg ctcaacgacc ttccacaagg tcgtccggct catcacctgt
     1861 tgcttgatcg tttcgattcg ctggtccgca taactcgtaa tcgtcgagtg gacgagttca
     1921 gatggaattt cctgttcttc gatcaggatg gttgccatgg atttgtaggt cgggggccag
     1981 agaaatgccg ccgtgaggct gaggatcaag agaaccccac ccgtcgtgaa tgtcagcatt
     2041 cggcggcggc ggaatatcgc tagatagtct tgaagacctt gaatcggctc agaatgtctt
     2101 gatgggtgtg gagtggtcat aggtagctct tctaattcga aaacgccaat ttgggtggat
     2161 agtatgtcag catgaacatc gtggcgttgg acatcgccgg atcggagaag ccgtctacgt
     2221 cacgccagcc ataggtataa gaaagctcag ccttccacca gtccagaaac ttccaggcga
     2281 tcttaggcgt gaggtagaag taacggctat ccgggaaagt gccgccttgc gcgcggtttg
     2341 taacacctga gatgagataa ccggatccat tgacggatgc agccagggtt tcggtcagat
     2401 catgcgacac tgatagacct gcacgatctc tttgaataag aaggccaaac ccgctcggca
     2461 caatatcccg gtccacattc aattggacag atgtacgctc aaacttcttg tcgagactgg
     2521 caccgaagag ccagactgtg tcctgggctt tgacattctc actgatggtt tgcgtagttg
     2581 aactcacaaa ccgcggtcca ccgtagattg tcccggtcag ggtttctgta aaggcatggg
     2641 tcaggttgag attgattccg ggaaagctgg cgcggaaggg cgagggtgca ttaatcgtat
     2701 ggaagtttac atatgagccg gatagttgga tttgggtctg ttcggtcatc tgatagagca
     2761 acccacctga gccaccaaac agctgatagt ccaccaggcc aagatgcaag ccattctcgt
     2821 aagtggtgtc ggataactga ccactggatt ggaacgacaa tttttcagtg atgcgtctgc
     2881 tccaagatgg attcgctgtc cattggtttc gttgtgtaaa gctcaacacc aaaccggtgg
     2941 tcagcagttc gctcatcaac gtgttgtcac gattgaatcc gccagtaaag ccaagcacat
     3001 ccttatcggt tttataatgg atagttactg gcccatgaat attggtaaaa ttgttctgct
     3061 gcccaccgta gtacgacaca aagtcggccg cgattctgcc actcacctcc aagcgctctg
     3121 ttttccccgc gaactcggct gccggtgagg tccaatagcc ataggtctca tcgtgcggga
     3181 gtggggttag aagcaaatta ctgttgtaca agcctttgac tccaatcgac ggtaagagtg
     3241 accactccgc agcttcgctc agggatgcca tgccaaatcc tagaatgcag gtccatagaa
     3301 tgagcttccc aactccattc cttcgacgag atcgcccacc acatgcagca tcatgccttg
     3361 atcccattgc tgccacgatt gctagggcac cacgacgaca tcaccgcgct gtaagagaa
//
LOCUS       DATIPF010000447         2900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_258611, whole genome shotgun sequence.
ACCESSION   DATIPF010000447 DATIPF010000000
VERSION     DATIPF010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258611"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VLL06_11075"
     CDS             <1..695
                     /locus_tag="VLL06_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HSS31550.1"
                     /translation="LLLDVTPLSLGIETMGGVMSSLIRRNTTIPASAKEMFTTYVDGQ
                     TGVDIHILQGERELVKDNRSLARFRLKVPPLPAGVPRIEVTFLIDANGILNVSARDVR
                     TGDTQSLAVKPSYGLSDDEVERMIGESYKFAAGDLKARQLIEARTEADAILMATDKAL
                     KQGSHLVGKEELSAIKTAIAEVVSTKSGDDHKAIRAKMAGLETATHHLAEVLMDTSLK
                     EALKNKKLSEIT"
     gene            751..963
                     /gene="iscX"
                     /locus_tag="VLL06_11080"
     CDS             751..963
                     /gene="iscX"
                     /locus_tag="VLL06_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HSS31551.1"
                     /translation="MDLKWQDAEDIAIRLVEEHPDSDPLTIRFTDMHAWIMALPDFKD
                     DPKKSNEKILEAIQMAWHEEFQDSKS"
     gene            complement(1216..1878)
                     /locus_tag="VLL06_11085"
     CDS             complement(1216..1878)
                     /locus_tag="VLL06_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSS31552.1"
                     /translation="METLIDDVSINHIECMAAPHAGPGIMLLTASMQLLYKNRCATEL
                     CRKIIQCQDGKTANGVLPPAIASLVDEMCQILEVQADPKDWEHIQFSRYVNMPHCSVL
                     LCGTALIDQSTAEARIVIVLSEVGIGAWENNVIVQAKEKFHLTARETTVLQHLLKGWT
                     NKEIANEMRLAEQTVKEHFKHIAKKTGITSRTGLAIKLCYLGLYTNPAIPSLRMAMMD
                     RA"
     gene            complement(2198..2512)
                     /locus_tag="VLL06_11090"
     CDS             complement(2198..2512)
                     /locus_tag="VLL06_11090"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSS31553.1"
                     /translation="MHARYYYMVTLGTTLVIGGALMTGPANAHDKDNKISNHARGGEG
                     IGKHERVLEMRQHDVRPLNDHAHNARTPVSVPEPSTLLLLGSGLVGLALRHFRKRKTD
                     TP"
     gene            2639..>2900
                     /locus_tag="VLL06_11095"
     CDS             2639..>2900
                     /locus_tag="VLL06_11095"
                     /inference="COORDINATES: protein motif:HMM:NF028228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS31554.1"
                     /translation="MTSVELLHGLPALSAAAASIEDVAIDPRDRLNLTVRILTAAITE
                     VARVGDQPLTTIGDVPAADTELRVAAEAAYRDLATLTSDRQTR"
BASE COUNT          651 a          787 c          777 g          685 t
ORIGIN      
        1 tcttgctgtt ggacgtcact ccactctccc ttggcataga aaccatgggc ggggtgatga
       61 gcagcttgat tcgacgcaac acaacgatcc cagccagtgc caaggaaatg tttacaacct
      121 atgtcgacgg ccaaaccggc gtggatatcc atatcctcca aggcgaacga gaactggtga
      181 aggacaaccg cagtctggcc cgctttcggt taaaagtccc tccgctgcca gccggcgtcc
      241 cacggatcga agtcacgttc ttaatcgacg cgaacggaat cctgaatgtt tcagcccggg
      301 atgttcgcac cggcgatacc caatcgctcg ccgtaaaacc atcgtatggc ttgagtgacg
      361 acgaagtcga acggatgatc ggtgagtcgt acaagtttgc ggccggcgac ttgaaagcac
      421 gccagctcat tgaagccaga accgaagctg acgccatcct gatggccacg gacaaggcgc
      481 ttaagcaagg ctcccaccta gtggggaaag aagaactatc cgcgatcaaa accgcgatcg
      541 ccgaggtcgt gtcaacaaaa tcaggagacg atcacaaggc tatcagagcc aagatggccg
      601 ggctcgagac ggccacccat catctcgccg aagtcttgat ggacacgtcg ttgaaggaag
      661 ccttgaaaaa taaaaaactt tccgagatca cgtgacgcgg gtctcgcagc gcttcatgaa
      721 gaacgaaccg caaaatacga ggaatgactc atggatttaa agtggcagga tgccgaggat
      781 attgcgatcc gtttggttga agaacatccg gattctgacc cactgaccat tcgattcacc
      841 gacatgcacg cttggatcat ggcactccca gacttcaagg acgatccaaa aaaatcgaac
      901 gaaaaaatct tggaagcgat tcagatggcc tggcatgagg aatttcagga ttcgaagtcc
      961 tgattgtatc tgaggggatt gctcaagtcg ttgatgattg ggcaatcccc cttctcctgc
     1021 cgaaccggta gcgaagatgc tgagtcagtt ctctttgttg ctagtcggtc tgtttcagcc
     1081 actttgcctc ttttcttaac actggaataa ctcctgctcc ctcacttggt gcaccacccc
     1141 cgccgatggg tttctgggtc ccatatgcgc cactcagcca accagcaaaa agggggttga
     1201 gccaaccgtg tcgtgttagg cacggtccat catagccatg cgaagtgatg gaattgctgg
     1261 attagtatat aatcctaaat aacatagttt aatggcgagt cctgtgcgtg aggtaatgcc
     1321 tgttttttta gcaatgtgtt tgaagtgctc cttgacagtc tgttctgcca gtctcatttc
     1381 attggcgatt tccttattgg tccagccttt caagagatgt tgaagcacgg ttgtttcgcg
     1441 tgcggtgagg tgaaatttct cctttgcctg gacaatgacg ttgttctccc atgcgccgat
     1501 tccgacttca ctgagcacga tgacgattcg tgcttccgca gtgctctgat caattaacgc
     1561 cgtgccacat aggagcacag aacaatgggg catgttcaca tagcggctga attgaatgtg
     1621 ctcccagtcc ttcggatcgg cttgcacttc gaggatctga cacatttcgt cgacgagact
     1681 ggcgatggct ggtgggagca ccccatttgc cgttttcccg tcctggcatt gaatgatttt
     1741 gcggcataat tccgttgcgc accgattctt atagagtaat tgcatggacg ccgtgaggag
     1801 cataatgccg ggcccagcat gcggggccgc catacattcg atatggttta tggacacatc
     1861 atctatgagg gtttccacgg gcttctcctt gtctcattcc tggccgcata gttggattca
     1921 tcatgacgac ccgctctcat gatgggtatt cctcttggaa ccagaagaag gatgctcctg
     1981 cttctggatt agtctagcga tggaagggaa gggatgcatg gatcatgcag ccgtggtgac
     2041 tcacggcgag actccacatg ccttggcaag gagcccatgt acggcgagtc accctgccac
     2101 gatgctccag ccgatgcgat ggccctaaca gcaccaacgg atttggatcc ctgcggagct
     2161 tcattcccgt caatagatct gctctttttg agcgcggtta tggcgtatct gtcttccgct
     2221 tccggaagtg ccgtagggct aaacccacta agcccgaacc gagcagcaac agtgtcgaag
     2281 gctctggaac agagaccggg gttctcgcat tgtgtgcgtg atcgttcaat ggtcgtacat
     2341 catgttggcg catttcgagt acgcgctcgt gtttcccgat gccctcgcct ccgcgggcat
     2401 ggttcgatat cttgttgtcc ttatcatgag cgttcgccgg tcctgtcatg agtgctccac
     2461 cgataaccag cgtcgtgccg agtgtcacca tataataata acgtgcgtgc atgttctcct
     2521 cctaaatgaa catgtggaat gacttacctg ctttatatcc gagtgatgat cgaaggccta
     2581 tcgacccacc taggatgttt ctgtcgttta aacacaaatc agcgatgtcg tggtttgtat
     2641 gacatcggtg gagctactgc acgggctccc tgcgctctct gcggccgccg ccagcatcga
     2701 ggacgtcgcg atcgatccgc gcgaccggct caacctcacg gtgcggatcc tcaccgccgc
     2761 gatcaccgag gtcgcgcggg tcggcgacca gcccctgacg acgatcggtg acgtccccgc
     2821 ggcggacacc gagctgcggg tcgcggcgga ggcggcctat cgggacctgg cgacgctcac
     2881 ctcggatcgc cagactcgga
//
LOCUS       DATIPF010000448         3948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_527858, whole genome shotgun sequence.
ACCESSION   DATIPF010000448 DATIPF010000000
VERSION     DATIPF010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527858"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..2947
                     /locus_tag="VLL06_11100"
     CDS             329..2947
                     /locus_tag="VLL06_11100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSS31555.1"
                     /translation="MSNEHDRFFDLSMDLLCIAGGDGYFKRLNPAWERALGFSREELY
                     SKPFMEFVHPDDRARTQVAMEQLSRGQAAINFENRYQCRDGSYRWLTWVTPAPDHEPP
                     ILYAAARDITELKQMEEALRRANTELEQHVTQRTHELAATNAALRVAESWQRALIDSI
                     QGIVWERDARTWKFSFVSPQAERVLGYPVRHWLDEPDFWHTHIHPADRNWVMAYSLAS
                     SRENRRYSFEYRLIAANGRTVWVQDIVTVEVDEHGPAILRGIMVDITERKQAETALRE
                     SEARFRSLFEEVPIAYQSLDIEGRFLDVNQGLCALLGYSAEELLGKSFEDCWPPDVRV
                     LFPAGFLSFKRTGRATNQLRLRRKDGKEIIVILEGRIQRDPMGNFLRTHCILTDITDQ
                     KLIEENLRRSELFITSVLEHLPNMIFVKDAKDLRFVRFNKAGEELLGYRQHELLGKND
                     YDYFPQEEADFFTAKDRQVLRSGRMLDIPEEPIQTKAKGVRFLHTKKIPICGADGTPQ
                     YLLGISEDITERKQAQEERGRQELLVTLMLSTGPGCIKRIAADGTLLQMNSAGLKLIE
                     ADCEKDALGLSVFDLVVPEHRASFMEMHRDVIHGSPRALQFEIVSLKGTRRRMETNGV
                     PFQNPVSAQTEHLAVTHDITERYQAEEALRASEEQLRNVLEEREQLSQDLHDNIIQSI
                     YATGMGLEECRYLFHEDPQGADKKLEQAVANLNRVISDVRIYIMWDKYEEITGPQFCT
                     KLAELAVTMESMQGIHFALDLAVEAAQRLTPREALHILHIVQEAMSNSLRHSQSRRGL
                     VSLQLQGGRIRLEIRDDGVGFNPEEALGRGHGLQNMRARAQKLGAPLQIVSQSGSGVQ
                     ILVELPLEDQHARASS"
     gene            2928..3587
                     /locus_tag="VLL06_11105"
     CDS             2928..3587
                     /locus_tag="VLL06_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSS31556.1"
                     /translation="MLEQAVRPIRLLLVDDHEVVRMGLGTLFHRAGTVEVVGEATSVV
                     SAVAEAIRLRPDIVLLDLRLPDGSGIEACREIREACPESHVVFLTSFADENAVLSTTF
                     AGAKGYLLKTIGGTALVHAIQAIADGHTILDPAVTESVLARMRSLATPDPDGQSTPLS
                     PQERRILPLVADGKTNKEIATALGLSDKTIKSYLANIFDKLQVTRRTQVVAMFVKHHP
                     Y"
     gene            3757..>3948
                     /locus_tag="VLL06_11110"
     CDS             3757..>3948
                     /locus_tag="VLL06_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31557.1"
                     /translation="MGTSNIKMGNSLQIVPSKQYDATRLALVEGLTIIIFLLDLFFPL
                     GFAIWMLYLFPVWFTLRSHW"
BASE COUNT          895 a         1063 c         1130 g          860 t
ORIGIN      
        1 cgagcgctcc cttgacaaaa aacctctgtt cacgtacatc cactttggat ttcgcccatc
       61 tacaacgttc gtgatctgtt cagggtaaag atcgaggccg acatgcgcaa attgcagccg
      121 gcatgcctat tacttttacc cctcaactcg ataagtacct tctcatcttc ctcctatgtt
      181 agtcagtgtc cgggcatatc ataccaatcc agtctggatg gggggatgcc gtcacgagct
      241 ttggctgaca gaaaacttac gctttgccgt tcccatggca gaaagggcga gggagcagat
      301 cactcgagca aagggaggat ctcgtgaggt gagtaatgag cacgatagat ttttcgacct
      361 gtcgatggat ctcctctgca tcgcaggggg cgatggctat ttcaagcggc tcaatccggc
      421 ctgggaacga gcgctggggt tcagccgaga agagctgtat tcgaaaccct ttatggagtt
      481 cgtgcatccc gatgaccgtg ctcgtacaca agtcgccatg gagcaacttt cacgggggca
      541 ggcggccatc aacttcgaga atcgatatca gtgccgggac gggtcctatc gatggctgac
      601 ctgggtcact cctgctccgg accacgagcc cccgatactg tacgcggcgg cgcgcgatat
      661 tactgaactc aagcagatgg aagaggcgct gcggcgggcc aacactgagt tggagcagca
      721 cgtaactcag cggacgcacg aactggcggc gacgaacgcc gcccttcgtg tcgcagaatc
      781 atggcagcgc gcgttgatcg actcgattca gggcatcgtg tgggaacgcg atgcgcgaac
      841 ctggaagttt tcgttcgtca gtcctcaagc cgaacgagtg ctcggctacc ctgtgcggca
      901 ctggctcgac gagccggact tttggcacac ccacatacat ccggccgacc gaaactgggt
      961 gatggcatat agcctcgcct cttcccggga gaaccgcagg tactcgttcg aataccggct
     1021 gatcgcagcc aatgggcgaa cggtgtgggt ccaggacatt gtgacggtgg aggtcgacga
     1081 acacgggccc gccattctgc gaggtatcat ggtggacatc accgagcgga aacaggcaga
     1141 aacagcattg cgcgaaagcg aagcgcgctt ccgaagtctg ttcgaagaag tgccgatcgc
     1201 ctatcaatcg ctggacatcg agggacgttt tctcgatgtc aatcaggggc tgtgtgcctt
     1261 actcggatat tccgctgagg aactgctggg caagtctttc gaggactgct ggccccccga
     1321 tgtgcgggtg ttgtttcctg ctggatttct cagttttaag cgaaccggcc gagccacgaa
     1381 tcagctaaga ctcaggagaa aggacgggaa ggaaatcatc gttatccttg aaggacgtat
     1441 ccagcgcgac ccgatgggaa actttctaag gactcactgt attctgaccg acatcacgga
     1501 tcaaaaactg atcgaggaaa acctgcgtcg ctccgagctc ttcatcacct ccgtccttga
     1561 acatctcccc aatatgatat ttgtgaagga tgccaaggat ctccgttttg tccgtttcaa
     1621 taaggccgga gaagagttgt tgggctatcg ccagcatgaa ctcttgggca agaacgacta
     1681 cgactacttc ccgcaagagg aagccgactt tttcaccgca aaggatcggc aagtcctacg
     1741 gtccggtcgc atgttggata ttccggagga gcccattcag accaaagcta agggagtgcg
     1801 gtttctccac acgaaaaaaa tcccgatctg tggcgccgac gggacgccac aatacttgct
     1861 gggcatctcg gaagacatca ccgagcgcaa acaggcgcag gaagagcggg ggaggcaaga
     1921 gctcctcgtc acactcatgc tgagcacggg gcctggctgt attaaacgca tcgcggccga
     1981 tgggacgttg ctccaaatga attcggctgg tctcaaactg atcgaggcgg actgtgagaa
     2041 ggacgccctc ggcctttcgg tgttcgatct ggtggttccg gagcaccgag cttcatttat
     2101 ggagatgcat cgagacgtca tccatggttc tccgcgtgca ctccagtttg aaattgtgag
     2161 tctgaaaggc acccggcggc ggatggaaac caatggcgtt cccttccaga atcccgtcag
     2221 tgcgcagacg gaacacctgg ctgtgaccca tgacatcacg gagcgctacc aggctgaaga
     2281 ggccctacgg gcgagcgaag aacagcttcg caacgtgctc gaagagcggg agcagctctc
     2341 ccaggacctc catgacaaca tcatccaaag catctatgcg acgggcatgg gccttgagga
     2401 atgtcgctat ctctttcacg aagatccaca gggggccgac aagaagttag aacaggccgt
     2461 cgctaactta aatagagtta tttcagatgt gcggatttat attatgtggg acaagtatga
     2521 ggaaatcacg ggaccacagt tctgtacgaa gctggccgaa ctggccgtga ccatggagag
     2581 tatgcagggg attcattttg ctctcgatct ggctgtggag gccgctcagc gtttgactcc
     2641 tcgggaagcg ctgcatatcc tgcacatcgt gcaggaggcg atgagcaata gccttcgtca
     2701 ctcacaaagc cgaaggggcc tcgtctcttt gcagttacaa ggcgggcgca ttcgtcttga
     2761 aatcagagac gacggtgtcg ggtttaaccc tgaggaggcg ctcggaaggg gacacgggtt
     2821 acagaatatg cgagcacgtg ctcagaagct tggtgcgcct cttcagatcg tctcacaatc
     2881 tggatcaggg gttcagattc tcgtcgaact cccgctggag gatcagcatg ctcgagcaag
     2941 cagttagacc gattcgattg ctcctcgtgg acgaccatga ggtcgtccgc atgggtcttg
     3001 ggacattgtt ccacagagcg ggtacggtgg aagtcgtggg cgaagccact tccgtcgtct
     3061 ctgctgttgc cgaggcgatc cgtctcagac ctgacatcgt cctcctcgac ctccgcttgc
     3121 cggatggcag cggcattgag gcatgccgcg agattcggga ggcatgtccc gagtcacatg
     3181 tcgtgtttct cacgtctttt gcggatgaga atgcggtcct gtcgacgacg tttgcgggcg
     3241 cgaaagggta cctcttgaaa acgatcgggg ggaccgcatt ggttcacgcc attcaggcca
     3301 tagccgatgg gcacactatt ctcgatcctg ccgtcacgga atctgtgctc gctcgcatgc
     3361 gatccctcgc aaccccggac cccgacggtc aaagcacacc attgtctccc caagagcggc
     3421 ggatcttgcc attggtggct gacgggaaga cgaacaagga aattgccacc gccctcggac
     3481 tgagcgacaa gaccattaaa agttatctgg ccaacatctt tgataagctg caagtcaccc
     3541 gtcgcacgca agtcgtcgcg atgttcgtca aacaccatcc ctactagagt cattcggcgg
     3601 tagttgagta gcgctgaaag atatcaacag gtaccgctca gcgctgatac cgctttacct
     3661 agttctcaag gatactgctg agcgtgggaa tctgattcca tagcatggta tcgcgggaag
     3721 ggaagcgaag atcggccttg ctggcaagag gaaggagtgg ggacctctaa catcaaaatg
     3781 ggcaactctt tgcaaatcgt tccttccaaa cagtacgacg cgacgcgtct ggctttggtg
     3841 gagggactca cgataattat tttccttctc gatctgttct tccccttggg ctttgcgatc
     3901 tggatgctgt atctcttccc ggtctggttc acactgcgtt ctcattgg
//
LOCUS       DATIPF010000449         2864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1412643, whole genome shotgun sequence.
ACCESSION   DATIPF010000449 DATIPF010000000
VERSION     DATIPF010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412643"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VLL06_11115"
     CDS             <1..802
                     /locus_tag="VLL06_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HSS31558.1"
                     /translation="ISTIQDRKYAEKVEGRFAPTETGRTVNDFLLKGFPDLINVDFTS
                     HMEEELDEVEEGSKPWVTAVRGFYGTFTTDLEKAKTIPGPKDTVEPPTDIPCEKCGRM
                     MEIKWGRNGKFLACPAYKDKPPCKNTQNFEKLEDGTIKIIPKIELTTDEKCEKCSSPM
                     VVKTGRFGKFIACSAYPECKTTKPLALGVKCPQPGCGGDLVQKRTRKGRSFFACSKYP
                     ACEYALWDRPINKACPTCSAPFLIEKVSKQVGRSVQCRSEECGYREAG"
     gene            1077..1553
                     /gene="bfr"
                     /locus_tag="VLL06_11120"
     CDS             1077..1553
                     /gene="bfr"
                     /locus_tag="VLL06_11120"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HSS31559.1"
                     /translation="MKAKEGVINILNKILTADLTAINQYFVHAKMCENWGYARLHSKV
                     RERSIAEMKDADELIGHILYLDGVPNVQRMNTVQVGETVAEQLKLDLKAEQEMLTVLS
                     EGVVHCTKVADFTTRHMLEDMAKDVDQHIDWIEMQMETIKQVGLENYLAEQIKKES"
     gene            1944..2105
                     /locus_tag="VLL06_11125"
     CDS             1944..2105
                     /locus_tag="VLL06_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31560.1"
                     /translation="MSRLHNVLAFRVPAEMARPRARQVVMVSLNVFVHRIGLPSTPAI
                     SFYDRLWRE"
     gene            complement(2439..>2864)
                     /locus_tag="VLL06_11130"
     CDS             complement(2439..>2864)
                     /locus_tag="VLL06_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31561.1"
                     /translation="ERFRAIPVLADPTLTDLGVWNIFSNPEILNPQDKIRAILCDEVQ
                     PCPLSNTDLLDRAVARFKTPGLRDLSHSAPYMHNGQFLALENVIGFYKGVSDQARITP
                     PAAGSLLNGADALRGIALKLPDDRAPLVAFLKSLNEDYQ"
BASE COUNT          704 a          719 c          812 g          629 t
ORIGIN      
        1 tatttctacg attcaagatc gcaaatatgc cgagaaggta gaggggcggt tcgctcccac
       61 cgaaacaggg cgaactgtga acgattttct gctgaaggga ttcccggacc tgatcaacgt
      121 cgacttcacc tcacatatgg aggaggagtt ggatgaagtt gaagaaggca gcaagccgtg
      181 ggtgacggcg gtgcgaggat tttatgggac cttcacaacg gatcttgaaa aggcgaagac
      241 gattcctgga cccaaggaca cggtggaacc accgacagat attccctgcg agaagtgcgg
      301 gaggatgatg gaaattaaat gggggcgtaa cggcaagttt ctcgcctgtc ccgcgtacaa
      361 ggataagccc ccctgcaaga acacgcagaa ctttgagaag ctcgaggatg gcaccattaa
      421 gattattccg aagatcgaac tcacaacgga tgagaagtgt gagaagtgca gcagcccgat
      481 ggtggtgaaa accgggcggt ttggaaagtt catcgcctgc tccgcctatc cggagtgcaa
      541 aaccaccaag ccgcttgcgt tgggtgtgaa gtgtccccag ccaggctgtg gtggagacct
      601 cgtccaaaag cgcacgagaa aaggacgttc gttctttgcc tgcagtaagt atcccgcctg
      661 cgaatatgcc ctgtgggatc gtccgatcaa caaggcctgc ccaacctgca gcgccccgtt
      721 cctcatcgaa aaagtcagca aacaagtcgg tcgcagcgtt caatgccgca gcgaggagtg
      781 cggttaccgc gaagccggat aagttcgacc gggttcctat tccattccat cgtgcagtct
      841 cttctgccct gaaaacggga tgcggcctgc tcgtgcttcg tccgcgtgtc atgacgttgc
      901 gaaattgaga accagtgtcg ccaatgatga aagtcaaact caaggagtct cgaacgaccg
      961 agttgacgaa cccttcagac gtggtgataa taaaccgaag ggtcgacatg ttgtagtgaa
     1021 agtctcttac atgaaggccc ctgatggtct atcaaacttg agaagggaga acgatcatga
     1081 aagcgaaaga aggcgttatt aacattctca ataagattct cacggcggat ctgacggcca
     1141 tcaaccagta ctttgttcat gccaagatgt gtgagaactg gggctatgca cggctccata
     1201 gcaaagtgcg ggagcggagt atcgcggaga tgaaagatgc cgatgagctc atcggccata
     1261 tcctctatct agatggtgtg ccgaacgtgc aacgtatgaa taccgtgcaa gtgggagaaa
     1321 ccgttgccga gcagttgaaa ctggatttga aagccgaaca agagatgctc accgtgttaa
     1381 gcgaaggcgt ggtgcattgt acgaaggtgg ccgactttac cacccgacat atgcttgaag
     1441 atatggccaa ggatgtcgac caacatattg actggattga gatgcaaatg gaaaccatca
     1501 agcaagtggg actggagaat tacctggctg aacagatcaa gaaagagtcg tgagacgtcc
     1561 cttccgggca cccgcaattc tcccatgttg catgtaattc cagaaagtgg ggcgaaaggc
     1621 ttcaggagga agtgaggctc cccccttttc gaagtgaaag accacgactc ccagtccgtt
     1681 acagtgcatc tatacgccat ggtattgaac caagccatcg cagcgcaccc acatcattcg
     1741 agcgtgatcg ttccacatgt tctccgtccg tgagggttga gtgcgatgct attgcacact
     1801 catgcagccg attcacgcag accctgccac gtgaattcaa cgtgcaacca gttggctata
     1861 tggacagtgg atgtccggtc attgggttgc ctctggtgct gtgattccat cagcgcagca
     1921 accttggatt tccattggcg tccatgagcc gtttgcacaa tgtcttggcc tttcgagtac
     1981 cggcagagat ggcccgccct cgtgcccgcc aggtggtgat ggtctccctg aatgtcttcg
     2041 tccaccgcat aggtcttcct tccacaccgg ccatctcgtt ctacgatcga ctatggcgtg
     2101 aatgattgat aaagcagggc cagatgagcc cgcaatggat acccttcttt acgtgcaagc
     2161 cttcctatga ggcttccctg tgagcctgcc aatgtgtccc tgtgccatca aaacaaaagc
     2221 ccacagttgg tgaacaaccg tgggctctgg acttgccgtc ctttggccta gccggctgat
     2281 ccgtgattcg atcagcggca caacaattca acaatggtgg cgggagacct caggaggacg
     2341 actgggtccc ccgcctgtga agtgggagtg gtgcggtccc acttcgatgc cggtgcatgg
     2401 ggcgcccagg ctgtcgtgtg atccttcgtg acttacgtct attgatagtc ctcgttcaac
     2461 gatttcagaa aagccacaag tggcgcacgg tcatctggta gcttgagagc gatacctctg
     2521 agagcatctg cgccgttcag caacgaccca gccgccggag gggtaatacg ggcttgatcg
     2581 gagacaccct tatagaatcc gatgacgttt tccaatgcga ggaactgccc attgtgcatg
     2641 tatggcgcag aatggcttag gtcacgaagg cctggagtct tgaatcgggc gacggctcga
     2701 tccaataagt cggtatttga tagggggcag ggttgcactt catcacagag aattgctcga
     2761 atcttatcct gtgggtttag gatctccgga ttggagaaga tgttccatac gcccagatcg
     2821 gtcaaggtgg ggtcggcgag gacgggaatg gctcggaaac gttc
//
LOCUS       DATIPF010000450         4810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_84588, whole genome shotgun sequence.
ACCESSION   DATIPF010000450 DATIPF010000000
VERSION     DATIPF010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84588"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(340..618)
                     /locus_tag="VLL06_11135"
     CDS             complement(340..618)
                     /locus_tag="VLL06_11135"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HSS31562.1"
                     /translation="MKFVILGFDGPDGEAKRKIYRPAHLAKMEPLDAQGKVVLAGPLT
                     DKTGSLIVIEVDSLEDAQQFAREDPYTVHGVFERVEVHPFTQVFPKER"
     gene            complement(646..1059)
                     /locus_tag="VLL06_11140"
     CDS             complement(646..1059)
                     /locus_tag="VLL06_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HSS31563.1"
                     /translation="MAHDDEHAQYERIFTLSEANRLIPQLNSKLIAIQQAKAVLARTK
                     EDTKKANAQAEFGGGSTVGHLYILGLQQVSTNLQAIQELGILVKDLDLGLCDFPHVRD
                     GRVVYLCWKLGEQEVRWWHETTAGYKDRYPLESQA"
     gene            1614..2861
                     /gene="rho"
                     /locus_tag="VLL06_11145"
     CDS             1614..2861
                     /gene="rho"
                     /locus_tag="VLL06_11145"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HSS31564.1"
                     /translation="MHLAELKQKSIADLNEVARDLKIDGAPNLRKQELIFAILQAQTA
                     NNGVVFGEGVLETLPDGFGFLRAPDSNYLPGPDDIYISPSQIRRFNLRTGDIVSGQIR
                     PPKESERYFALLKVEKVNYEDPEVSRDKILFDNLTPLYPEERLVLEFDREEYCTRVMD
                     LTTPIGKGQRGLIVAAPRTGKTMLLQAIARAILKNHKEVTLIVLLIDERPEEVTDWQR
                     QVKAEVISSTFDEPAQRHAQVAEMVLEKAKRLVEHKKDVVVLLDSITRLARAYNTIAP
                     PSGKVLSGGLDSNALQRPKRFFGAARNIENGGSLTIMATALVDTGSRMDDVIFEEFKG
                     TGNMEVHLDRRLADKRLFPAIDISQSGTRKEELLVDKDRLNKMWILRKVLSPLGTMEA
                     MEFLMDKLHGTKTNQEFLQSMNR"
     gene            2956..3162
                     /gene="rpmE"
                     /locus_tag="VLL06_11150"
     CDS             2956..3162
                     /gene="rpmE"
                     /locus_tag="VLL06_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HSS31565.1"
                     /translation="MKKGIHPVYREVAVHCACGNKYKTRSTGGDINVDICSNCHPFFT
                     GTQKIIDTEGRVERFKKKYAKKGK"
     gene            3265..4341
                     /gene="prfA"
                     /locus_tag="VLL06_11155"
     CDS             3265..4341
                     /gene="prfA"
                     /locus_tag="VLL06_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HSS31566.1"
                     /translation="MEAALLKKWEVLAARYDELTNQLMDPSLMSQPSQLHKVNKERTD
                     IEPAAKLLATYRDNAKQLEEAAQILADPSAGVEMYKMAMEESSTLERQQHEIEQQAQE
                     LLIPKDPRDEKSLLLEIRAGTGGDEAALFAGALFRMYSRYAEVKGFKVDVVEATETGG
                     GGYKGVVALIEGKGAYSHFKFEAGVHRVQRVPVTEASGRIHTSTVTVAVMPEVDEVDV
                     QIDPMDLRIDTFCSSGAGGQSVNTTKSAVRITHIPTGVVVSCQDERSQLKNRNKAMRT
                     LRARIVEAERERQEAEIAQNRKAQVGTGERSEKIRTYNFPQNRVTDHRVGVTLHKLEQ
                     VLEGDLDEFVRALKAQRQQEVGAA"
     gene            4341..>4810
                     /locus_tag="VLL06_11160"
     CDS             4341..>4810
                     /locus_tag="VLL06_11160"
                     /inference="COORDINATES: protein motif:HMM:NF037644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31567.1"
                     /translation="MMDAVAPIPQSAGHATLGEVIAEARRMLERAGIESAEQEVFWIV
                     EHVLCLAAHHVVADRDRLLSQVERSAVRELIERRVSREPLQYILGTQEFCGLEFEVDP
                     AVLIPRPETELLVEYVAQRISLDQQATVVDVCTGSGCIAVAIARLRPRVRVIAS"
BASE COUNT         1157 a         1192 c         1440 g         1021 t
ORIGIN      
        1 tggtcggcgt ggtgctgccg ctcggtcgcg tcggcggcga cgcgggcgag gccgcggtgg
       61 gcgtgaccca ggtaccaggg ctcaccgatg cgctcgtaca gagcgagccc cggctaaggt
      121 gtcaccatcg cccatgacat attggccatc ggcgatgacg gttttcgttg cagcgctgaa
      181 cgtagggggg gcccatagaa ggagcgcgat aaggaagggg tagaacggtc gcctgtacag
      241 tcccattata gtaactatac cgcgcgatgg ctgaagcggc aacggaacca gaacggggca
      301 aaggagtagg ggtaaaagta gggaacgcca agccttcggc taccgttcct tcgggaatac
      361 ttgagtaaat gggtggacct cgacccgttc aaatacacca tggacggtat aggggtcttc
      421 gcgagcaaat tgttgcgcgt cttccagcga gtccacttcg atgacaataa gactccctgt
      481 tttatccgtg agcggaccgg cgagcacaac cttgccttgt gcatcgaggg gctccatctt
      541 ggcaagatgc gccgggcgat aaatttttcg cttggcttct ccatcggggc cgtcaaatcc
      601 gagaatgaca aatttcatac agtgttgaga gctaggattc tgacatcagg cttggctctc
      661 tagagggtag cgatccttat acccggcagt tgtctcatgc caccaacgaa cttcttgctc
      721 tccaagcttc cagcagagat agaccactcg accgtcacga acatggggaa agtcacagag
      781 gccaagatcc aggtccttta ccaatatgcc aagttcctga atcgcctgaa gattcgtact
      841 gacttgttga agccccaata tgtagaggtg tccaacggta ctgccaccgc caaactcggc
      901 ctgagcgttg gctttcttgg tatcttcttt tgtgcgtgcc aagacagcct tcgcttgttg
      961 aatcgcaatc aacttggaat tgagttgggg gatgaggcga ttggcctccg acaatgtaaa
     1021 tattcgctcg tattgtgcgt gttcgtcgtc gtgcgccaag atctgctccc gcgaagctct
     1081 tcatttagca caccactctt ttgcaccgca actgatagga gtcggagttc atactcgtgt
     1141 cgccaagtaa gaacaatttc gtggggacgg gctgaaagaa ggaggcacag cgtcggtaaa
     1201 agagatatct atattgacaa ggaatgtcgc tcgcgttatt attcaacata ctatctcggt
     1261 tcgattcata atttctccac acacaagact cccggggcgc atctcgatac aagtcacaat
     1321 tgtttactca gcgaacagag gcgaatcagc tcggcatgag ttccggcctg caacaatatc
     1381 cgcaaccatg agacagagtc gtaccctgat agccacgagc gaaaattagg taagtccctc
     1441 acgggttacc ctcttttagc tgttatacac gacacaatca tcaacgcagt accattacga
     1501 ttacgattca gtccgtggac aatttcccat ggttcaccac gtgcgattgc tgccgataac
     1561 aaagcggcag ggggcttgag aggatactca tgacacaaac gaagggggag gttatgcatc
     1621 tcgcggagtt aaagcagaag tccatcgcgg atctcaatga ggtggcgcgc gatctgaaga
     1681 tcgatggagc acccaatctg cgaaagcagg aattgatttt tgcgattctg caggcgcaga
     1741 ccgcgaataa cggggtggtt ttcggagaag gcgtactcga aacgctccct gatgggttcg
     1801 gatttcttcg cgctccggat tcaaactatc ttcccggtcc cgatgacatc tatatctcgc
     1861 cctctcaaat ccggcgtttc aatttgcgta ccggtgatat tgtgtccggg cagatccggc
     1921 cgccgaagga gagcgagagg tattttgcgt tgctcaaggt cgagaaggtc aattacgaag
     1981 atcctgaagt gtctcgcgac aaaattcttt tcgataacct gacgccgctc tatccggaag
     2041 aacggctggt cctcgagttc gaccgtgaag aatactgcac ccgagtgatg gatctcacga
     2101 ccccaatcgg caaaggccag cgcgggttga tcgtcgccgc tcctcgaacc gggaaaacga
     2161 tgttgctaca ggccatcgct cgggccattc ttaaaaacca caaggaagtc actctgattg
     2221 tcctgctcat cgacgaacgg ccagaagaag tcacagactg gcaacggcag gtgaaggccg
     2281 aagttatcag ttccaccttc gatgagccgg ctcagcgcca tgcccaggtc gctgagatgg
     2341 tacttgaaaa agccaagcgg ctggtcgaac ataagaaaga cgtcgtcgtg ctgttggaca
     2401 gcattacgcg cctggcgcgt gcctacaata ccattgcgcc gccaagcggc aaggtcttat
     2461 cgggaggatt ggactcgaat gcgcttcagc gtccgaagcg gttctttgga gcggcccgca
     2521 acatcgaaaa cggcggcagt ctcaccatca tggcgacggc acttgtcgat actggtagcc
     2581 ggatggatga tgttatattt gaagagttca aaggaaccgg caacatggaa gtccatctcg
     2641 atcgccgtct ggccgacaag cggctcttcc cagccatcga tatcagtcag tctgggacga
     2701 gaaaagaaga attgttggtg gataaggatc gcctcaacaa gatgtggatt ctacgcaagg
     2761 tgttgagtcc gcttgggaca atggaagcca tggagtttct catggacaag ttgcatggta
     2821 ccaagactaa tcaggaattc ctgcaatcaa tgaaccgata atatccgact tgtatccggt
     2881 cgccgagaca ggctagagtg tcgggccttc gaatgggcga aggctgatga tggtgaagtc
     2941 aggggagtgt gaactatgaa gaaggggatt caccccgtgt atcgggaagt cgcggtgcat
     3001 tgcgcctgcg gcaacaaata taagactcga tcgactggtg gcgatatcaa tgtcgatatt
     3061 tgttcaaact gccatccgtt ttttacgggg acgcagaaga tcattgatac cgaagggcgc
     3121 gttgaacggt tcaagaaaaa gtacgcgaaa aaaggcaagt agctcgagaa gaggccatat
     3181 gagggaccct gcttcgctga ggggcagggt ctctttgttt ttgtacatac acgaggccga
     3241 gagcgaatca ggggtaaacg ggccgtggaa gctgcgttat tgaaaaaatg ggaagtgttg
     3301 gcagcccgat acgatgagtt gacgaatcag ctcatggatc cgtctttgat gagccagccg
     3361 tcgcagctgc acaaagtcaa taaggagcgg acagatatcg aaccggcggc caagttgctt
     3421 gccacctatc gagacaacgc aaagcagctg gaagaagccg cgcaaattct tgcagatccc
     3481 agcgcagggg tcgagatgta taagatggcg atggaggaga gcagcacgct cgaacgccag
     3541 cagcatgaga tcgagcagca ggcccaagag ctgctcattc ccaaagaccc gcgagacgag
     3601 aagagcttac ttctcgaaat ccgtgccggg accggcggtg atgaggcggc gctctttgcc
     3661 ggcgcgttgt tccggatgta cagccggtac gctgaagtca aaggcttcaa agttgatgtg
     3721 gtcgaagcga cagaaaccgg gggtgggggc tacaagggag tcgttgcgct gatagaaggc
     3781 aagggcgcct acagccattt caagttcgaa gccggggtcc atcgtgtgca gcgtgtccct
     3841 gtcaccgaag cgagcggtcg gattcatacc tcgaccgtca ccgttgctgt tatgcccgaa
     3901 gtggacgagg tcgatgtgca gatcgatccc atggacctcc ggatcgacac cttctgctca
     3961 tccggtgccg gcggccagag tgtcaatacg acaaaatcgg cggtgcggat cacccatatt
     4021 ccgaccggtg tggtagtgag ttgtcaggac gaacggtcgc agctgaaaaa tcgcaataag
     4081 gcgatgcgga ccctgcgggc cagaatcgtc gaggcggagc gggagcggca ggaagcggaa
     4141 atcgcgcaga accgtaaggc tcaggtgggg acgggtgagc gaagcgagaa gattcgtacc
     4201 tacaacttcc cgcagaatcg cgtcactgat catcgcgtgg gtgtgacctt gcataagttg
     4261 gagcaggtgc ttgaaggcga cctggacgaa ttcgttcgag ctctaaaggc acagcgtcag
     4321 caagaagtgg gagcggctta atgatggatg cagttgcccc gatcccgcag tcggcaggtc
     4381 atgcgacact cggtgaggtg atcgccgagg cgcgacggat gttggaacgg gctggtatcg
     4441 agagcgcgga gcaagaagtg ttttggatag tggagcacgt gctctgtctt gctgcacacc
     4501 atgtggtcgc tgatcgagat cgactgttgt cacaggtcga gcggtcggcg gtgagagagt
     4561 tgatcgagag gcgggtgagt cgcgagccct tacaatatat tctggggacc caggagtttt
     4621 gtgggttgga gtttgaggtg gacccggcgg tactgatccc gaggccggag accgagttgc
     4681 tggtcgagta tgtcgcgcaa cggatttccc tcgatcagca agccaccgtc gttgatgtct
     4741 gcacgggatc cgggtgcatc gccgtggcga ttgcgcggct gaggcctcgt gtacgggtga
     4801 tcgccagtga
//
LOCUS       DATIPF010000451         2855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_970533, whole genome shotgun sequence.
ACCESSION   DATIPF010000451 DATIPF010000000
VERSION     DATIPF010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970533"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1059)
                     /locus_tag="VLL06_11165"
     CDS             complement(34..1059)
                     /locus_tag="VLL06_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HSS31568.1"
                     /translation="MPERDYYQILGLPKSASADDIKKAYRRLARQFHPDLHSGSKKSE
                     MEKKFKELNAAHEVLSDPDKRKKYDQFGANWEQADAYEQARRQAGARRESGPNTPFGA
                     EGFSDIFENLFRGRGRSGNGRGFAMQGEDLETEVQLTLAEVFTGVTKRVTLQEPVPCS
                     TCQGSGVLRGRPCPTCQGQGATLHPNTIEVRIPAGVQDGTRVRVAGKGQPGANGGKPG
                     DLYMRVMIAPDKVFRRQGSDIHVTLPVFPWEAALGAEVMAPTLMEPVRMKVPPGSRAD
                     NKLRLKGKGLPAANGGHGDLFLTIQIVMPPSSTDAEHKLYDQLRAIPHPDPRAELLAQ
                     ARRRSHP"
     gene            complement(1180..1806)
                     /locus_tag="VLL06_11170"
     CDS             complement(1180..1806)
                     /locus_tag="VLL06_11170"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HSS31569.1"
                     /translation="MTSLRAYFPTLTLKLGLILLLGMTASPSAHAKELPFATPVGSHT
                     FGLTTGPLFPLRLLSSQSSKLFGEALMPSWMITLTEPIGTGWYQGQVALGAELIAIHT
                     YEPITAYGAGFTPKLVYTSTAFGRVGPFVEAGGGPMWTDLGGRVPEQPGQFNFLVWAG
                     AGASYHMTPTWSLTAGYRIMHISNGGTRSPNSGLNFGLPFIGLSYQGF"
     gene            complement(1834..2769)
                     /locus_tag="VLL06_11175"
     CDS             complement(1834..2769)
                     /locus_tag="VLL06_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS31570.1"
                     /translation="MNCTKCKTKAVIGLPRHNAAFCKGCFNGFVHDQVARAIKSEKMI
                     AREDRILVAVSGGKDSLALWDILLKLGYRADALYVDLGIGAYSEQSHAKVTKFAEAVA
                     ASHSAVLHLHTVEQEAGAGIKELAQLIHRPTCSTCGTIKRYQFNRVAIDQHYDVMATG
                     HNLDDEAARLLGNVLHWQEEYLDKQGPSLPASVEGFAKKVKPLYRLSERELAAYAVLN
                     RIDYIVEECPMAKGSKMLQYKEVLNRLETESPGTKQTFYWGFLEKQSKKQPTATTMTE
                     KDRAVLHPCTSCSQPTTAEVCSYCKMMARAKTHAS"
BASE COUNT          566 a          790 c          837 g          662 t
ORIGIN      
        1 agaacgccgc tggcggactt tttcagcatc ctgctaggga tgtgatcggc gtcgggcctg
       61 agcgagcagt tcggcacgcg ggtcagggtg cgggatggct cggagttggt cgtatagttt
      121 gtgttccgcg tcggtcgatg agggcggcat cacaatttgg atcgtcagga acaggtcgcc
      181 gtggccgccg ttcgccgcag ggaggccctt gcccttcagc cgaagtttat tgtcggcccg
      241 gctccccggt ggaaccttca tccggaccgg ttccatgagc gtcggagcca tcacctcagc
      301 gcccaaggcc gcttcccacg ggaagacggg aagggtgaca tgaatgtcgc tgccttgccg
      361 gcggaaaacc ttgtccggag caatcatcac ccgcatatac aaatcaccgg gcttaccacc
      421 attggcgcct ggctgtccct ttccggccac gcgaacccga gttccgtcct gtaccccggc
      481 ggggattcgc acttcgattg tattgggatg cagtgtcgca ccctgtccct gacaggttgg
      541 acagggacgt ccgcggagga ctccactgcc ctggcaggtg gagcagggca cgggttcttg
      601 caaggtcacg cgtttcgtga caccggtaaa cacctcggcc agtgtcagct gcacttccgt
      661 ttcgagatcc tccccctgca tggcaaatcc acggccattc ccgcttcgcc ctcgtcctct
      721 gaagagattt tcgaagatat cggaaaagcc ttcagccccg aatggagtat ttggaccgct
      781 ctccctgcgg gcaccggcct gcctgcgcgc ctgctcgtac gcgtcggcct gttcccagtt
      841 ggcaccaaat tgatcgtatt tctttcgttt gtccggatcg ctcaacactt cgtgcgccgc
      901 attcaactcc ttgaactttt tttccatctc cgatttctta gacccgctgt ggagatcagg
      961 gtggaattgg cgcgcgagac gacgataggc cttcttgatg tcatctgccg aggcagattt
     1021 tgggagaccg agaatctgat agtagtcgcg ttcaggcata aaatcggtgc tctcgatatg
     1081 aacggtctac ggagaagata atgccgcagc ttacggggtc aatcttttct ttgcaagtta
     1141 tggacggtcc tgcgagagca atgggtgaag gaagcactgt cagaacccct gataggacag
     1201 tccgatgaag ggcaacccga aattaagtcc ggagttgggc gaacgggttc cgccattgga
     1261 gatgtgcatg atccgatagc cggcagtcag cgaccatgta ggagtcatat gataggaggc
     1321 gccggcaccg gcccacacca ggaagttaaa ttgtcccggt tgttccggca ctcgaccgcc
     1381 caaatccgtc cacatggggc ctccgcccgc ctcgacaaac gggcctaccc ggccaaaggc
     1441 cgtggaggta tagaccaact taggtgtgaa tcctgcgccg taggccgtaa tcggttcgta
     1501 ggtatgaatg gcaatgagtt ccgcaccgag tgctacctga ccttgatacc agccggtccc
     1561 gatcggttct gtgagtgtga tcatccatga tggcatgagc gcttcgccaa agagtttgga
     1621 tgattggctc gacagaagcc gcaggggaaa taatggacca gtcgtcagtc cgaatgtgtg
     1681 gctgccgacg ggtgtggcga agggcaattc ttttgcgtga gctgagggtg atgccgtcat
     1741 gcccaataag aggatgagtc ccagtttgag tgtgagggta gggaaatatg ctctgagaga
     1801 ggtcacaatc ggttctcagg tagtgggccg gttttacgac gcgtgggtct tggctcgcgc
     1861 catcatcttg cagtaggaac agacttcagc tgtggtgggt tggctgcaag aggtgcaggg
     1921 gtggaggacg gcccggtctt tctccgtcat ggtggtcgca gtaggctgct tcttactttg
     1981 cttctcaaga aaaccccaat agaatgtctg cttcgtgcct ggcgactcag tttccagacg
     2041 atttaagacc tccttatatt ggagcatctt ggagcccttg gccatggggc attcctcgac
     2101 gatgtaatcg attcgattca acaccgcata ggccgcgagt tctcgttccg agagccggta
     2161 caggggcttc accttcttgg caaacccttc caccgaggcc ggcaacgacg ggccttgctt
     2221 atccaggtat tcctcctgcc aatgcaacac gttgcccagg agccgggccg cttcatcatc
     2281 gagattatgc cctgtggcca tgacatcgta atgctggtcg atcgccacgc gattgaattg
     2341 atagcgtttg atcgtcccac atgtcgagca ggtcgggcga tgaatgagtt gggccaactc
     2401 cttgatcccc gctccggcct cctgttcgac cgtatggaga tgtaacacgg cgctatgcga
     2461 ggcggcgacc gcttcggcga acttcgtcac cttggcatgc gattgttcgg aataggcccc
     2521 gatgccgagg tccacataga gcgcgtctgc gcgatagccg agcttgagca gaatgtccca
     2581 gagggccaga ctgtctttcc cgcctgacac cgccacgaga atcctgtcct ccctggcaat
     2641 catcttctcc gacttgatcg ctcgcgcaac ctgatcgtgc acgaagccgt tgaagcaacc
     2701 tttgcagaag gctgcattgt ggcgagggag tccgatcacc gctttggttt tacatttggt
     2761 gcaattcatg ggctggtctc tctggttagt ttggtctgtt tggtttattt ggtttggacg
     2821 agaacaacca gataaaccaa gcaatccaga tgacc
//
LOCUS       DATIPF010000452         2594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_528600, whole genome shotgun sequence.
ACCESSION   DATIPF010000452 DATIPF010000000
VERSION     DATIPF010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528600"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VLL06_11180"
     CDS             complement(<1..491)
                     /locus_tag="VLL06_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31571.1"
                     /translation="MAKIAVIDIGTNSIHMVLAEIQPDASYKILDRFKDMTRLGNGAF
                     TTHRLSDEAIARGLDVIRNLVTLAKNKGYERMIAVATSAVREAKNGGDFIDLVMEQTG
                     LKVRVISGAEEARLIFLGVKNNVPMTEAPTLSVAPAHGQRPQIQGVGLRARAVAAAAD
                     LVHG"
     gene            723..1607
                     /locus_tag="VLL06_11185"
     CDS             723..1607
                     /locus_tag="VLL06_11185"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSS31572.1"
                     /translation="MVLSPMSNRLSSMAVVVTGASTGIGAACALDFAGRGMTVFAGVR
                     DPRAGEALAAKGGPSLIPITLDVTDEPSIARSVETVQRVVGDSGLGGLVNNAGIAIGS
                     PLEVIDLSQLRKQLEVNVIGQIAVTQAFLPLLRRARGRIVNMGSIAGRGTIPLLGPYS
                     ASKFALEALTDALRMELQPWGIHVSIIEPGAIATPIWEKSEKAAGDLEASASAEAKAL
                     YGESVIRIREAIVQATKRAIPPAAVVHAVHHALTASHPHTRYLVGADAKARAWMVKWL
                     PDRVQDRLLTWALKYPQA"
     gene            complement(1736..2173)
                     /locus_tag="VLL06_11190"
     CDS             complement(1736..2173)
                     /locus_tag="VLL06_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31573.1"
                     /translation="MATPSNIQPPHQDARTPCVLTLLLSLIAFLAAPWYVHAGSLLEI
                     TELLSHPEQYDRQEVVVTGQVTNVQLATNREGHPAYGFLLKDHAGTLKIISLGQVEVR
                     EGDQVIVEGVFSRLRQAGRTIIYNEIKALSVKPMNRLNPDLVG"
     gene            complement(2272..>2594)
                     /locus_tag="VLL06_11195"
     CDS             complement(2272..>2594)
                     /locus_tag="VLL06_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31574.1"
                     /translation="GDLAEESEVKFWTSLVFGASSNAMHSEVTRNLFGLQRLITVLND
                     MDHSPEAVDEELIKRTAAKPKLEVGTLEAPAEAPAKELSEMQAQILNSMRGEDVAGRP
                     SDKW"
BASE COUNT          523 a          690 c          775 g          606 t
ORIGIN      
        1 ccgtgcacga ggtcggcggc cgcggctact gcacgggcgc gcaggcctac accttggatc
       61 tgcggacgct ggccgtgggc cggcgccacc gaaagcgtcg gcgcttctgt catgggcacg
      121 ttgtttttga cccccagaaa aatcagcctg gcctcttcgg cgccgctgat gacgcgaact
      181 ttcagccccg tctgttccat cacaaggtcg ataaagtctc ccccattttt tgcctcgcgc
      241 acggcactgg tggcgactgc gatcatgcgt tcgtatccct tgttcttggc cagcgtcacg
      301 agattgcgaa tgacgtcgag cccgcgtgcg atggcctcgt cggagagacg gtgggtcgta
      361 aaggcgccgt ttccgagtct tgtcatgtct ttgaagcgat cgaggatctt gtagctggca
      421 tccggctgga tctcggccag caccatatga atggagttgg tcccgatatc gatgaccgca
      481 atcttcgcca tgaacagtga tacctatcag atacctagag ttgagaccct gctcgataca
      541 taggcgcatt cgctggagta tttccgctcg tgtcgagtga acatgttaca tcaactgggt
      601 gggaatattt tcgtcgatgg ctggaccagt cagaaaccat aatgaatgat cccgtggcct
      661 gtcaaggtcg ccagggttaa ttctgggtta catacgcgga agctgctctc gtgcatcgtg
      721 ccatggtact ctcgcctatg tccaatcggc tgtcgtccat ggcggttgtt gtgaccggtg
      781 cctcgaccgg cattggtgca gcctgtgcac tcgattttgc cggccgtggg atgacagtgt
      841 ttgccggtgt gcgtgatcct cgcgcagggg aggcgctggc agcgaagggc ggtccatcac
      901 ttattcccat cacactcgat gtcaccgacg agccgtcgat cgcgcgatcc gtcgagacgg
      961 tgcaacgggt tgtcggcgac agcggccttg gagggcttgt caataatgcc gggatcgcca
     1021 tcggtagtcc tctcgaagtg attgatttgt cgcaactccg aaaacagctt gaagtcaatg
     1081 tgatcggaca aatcgcggtc acgcaggcgt ttcttccgct gctgcgccgg gcccgcggtc
     1141 gaattgtgaa tatggggtcg attgccggcc gtggcaccat tccattgttg ggaccctatt
     1201 ccgcatcgaa gtttgcattg gaggcgttga ccgatgcgtt gagaatggaa ctgcaaccct
     1261 gggggattca cgtgtcgatc atagaaccgg gggcgatcgc gacgccaatt tgggagaaat
     1321 cagagaaggc ggcgggggac cttgaagcgt cggccagcgc agaggcaaaa gcgctctatg
     1381 gcgagtcggt gattcgaatt cgtgaagcga tcgttcaagc gaccaaacga gcgattccac
     1441 cggctgcggt agttcacgcg gtgcatcatg cgttgaccgc atctcatcct cacacgcgct
     1501 atctcgtggg cgcggatgcc aaggctcgtg catggatggt gaaatggtta ccggatcgag
     1561 tgcaagacag gttgctcacg tgggcgctga aatatccgca agcgtaagaa gatggtctat
     1621 ctggtctgtc tcgtctatct tgtcggtctg gttagtttcg ttgtcgagat caccgatcaa
     1681 acgagataga ccgaatagac cagatagact aaatagacca gacagactag agagatcaac
     1741 caaccagatc ggggttgagg cggttcatcg gcttgaccga gagggctttg atttcgttat
     1801 agataatcgt gcgtcctgcc tgccgaaggc gtgagaagac tccctccaca atcacctggt
     1861 cgccttcccg aacttcaacc tgaccgagac tgataatctt cagcgtgccg gcatgatcct
     1921 tcaggagaaa cccataggcc gggtgacctt cccgattcgt ggccagttgg acattggtga
     1981 cttggccggt gaccaccact tcttggcggt catactgctc gggatgggag aggagttcgg
     2041 tgatctccag aagactgcct gcgtggacat accacggtgc agccaggaag gcgatgagtg
     2101 aaaggagaag agtcaggacg catggggttc gcgcatcctg atgcggtggc tggatgttgg
     2161 acggagtggc catgagttga atgtattata cgggttcaac ggcaaagtgc aatcatgtta
     2221 cgccgagcat gcaaaaatta tttctgcgcg tctcgtcatg aatcatacgg cctaccactt
     2281 atcggagggt cttccagcta catcttctcc tctcatactg ttgaggatct gagcctgcat
     2341 ctcgctcaat tcttttgccg gagcctcggc aggggcttcg agcgtgccca cctccagctt
     2401 gggtttggcc gcggtccgct tgatcaattc ttcgtccact gcctcgggtg agtggtccat
     2461 atcgttgagg accgtgatga gccgctgcag tccaaacagg ttcctggtga cttctgaatg
     2521 catggcattg gagctggctc cgaagacgag cgaggtccag aatttgactt cactctcttc
     2581 ggccaggtcc cccc
//
LOCUS       DATIPF010000453         5091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_529055, whole genome shotgun sequence.
ACCESSION   DATIPF010000453 DATIPF010000000
VERSION     DATIPF010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529055"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VLL06_11200"
     CDS             complement(<1..207)
                     /locus_tag="VLL06_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31575.1"
                     /translation="MLKKFVQQGRRRVETGGVPSGAHGATNKERSSTGTPAFAAQLRM
                     STIERQHPEPRLPFPGQSTPTVSVV"
     gene            306..575
                     /locus_tag="VLL06_11205"
     CDS             306..575
                     /locus_tag="VLL06_11205"
                     /inference="COORDINATES: protein motif:HMM:NF021169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HSS31576.1"
                     /translation="MAQSQSDTVHVFDTWVRGAKGLLHFDVMTTDEATALRLAKQHLA
                     SLGEGAVPVTVKECQFCHTEPLVMFNSEQQRQFREQGGFIITLSA"
     gene            complement(870..1391)
                     /gene="lspA"
                     /locus_tag="VLL06_11210"
     CDS             complement(870..1391)
                     /gene="lspA"
                     /locus_tag="VLL06_11210"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HSS31577.1"
                     /translation="MGRPLRYLLLALLSGTIIVIDQATKLSIVEAMHLNESIPVVPNL
                     FSLTYIRNPGAAFGLLAGSSDAFRMVFFGVTSLFALALLGTILYRLPEKDWVGQLSIA
                     GILGGAIGNLIDRLRYGEVIDFLDVYVDSYHWPAFNVADSAISVGVVFLIIHFAFEKK
                     NVPLRPQELPPAS"
     gene            complement(1391..4210)
                     /gene="ileS"
                     /locus_tag="VLL06_11215"
     CDS             complement(1391..4210)
                     /gene="ileS"
                     /locus_tag="VLL06_11215"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HSS31578.1"
                     /translation="MDYKATLNLPKTDFPMKANLPLREPELLASWEREGLYQQIQEAG
                     KGRPRYILHDGPPYANGRIHIGHALNKILKDIIIKSKTMAGYQVPYVPGWDCHGLPIE
                     HQVLKELGDKKKTLDTPAIRRLCREYAEKFFIIQREEFQRLGILGDWEHPYLTMNPGY
                     EATIIREFGKFVERGGVYKGLKPVLWCTVDQTALAEAEVEYEDHTSPSIYVKFPLVTS
                     PTVLSQTFPGVVFPGGMKLVSVVIWTTTPWTLPANQAVCLHGDIDYAFVQVGDEVLIV
                     AEKLVESVVKACSLEGARVFGVKKGKEGFEGIETQRPLTTGLSPILLGDFVTIDQGTG
                     CVHIAPGHGMEDYILVIEHNSKATPGERLEILAPVDNAGRFTDVVKEFAGQHVLKANP
                     KIVEFLKANGRLLGHGSLNHSYPHCWRCKNPVIFRATEQWFVSMEINDLRKEALAEID
                     RVQWIPAYGRDRINGMIENRPDWCLSRQRVWGVPIPGFTCIGCRAVLADPNVIEHIAT
                     LMESKGADVWFEQSAADLLPKGTSCSNCNGTAFEKERDILDVWFESGVSYAAVLKERK
                     WWPADLYLEGSDQHRGWFHSALLAGVVTDHRAPYKAVLTHGFVLDGQGKKMSKSAGNV
                     VAPQDVIKQSGAEILRLWVSAQDYRDDVRISPEILTHLIEAYRKIRNTCRFLLSNLYD
                     FDPDKDRIPYEQLPELDRWALHRLSELIPRVQKSYEDFEFHTIFHALNNFCSVDLSAV
                     YLDILKDRLYTFRADSPLRRGSQTVLFDIVVAMTKLMAPVLSFTAEEIWRTLAAQPGG
                     HLGVSSVHLSTFPEIQSQWQDAALAERWERLLKYRSQVQGVLEERRREKVIGSSLEAQ
                     VHLTVDAKTGMWITSACRDLATLFIVSEVTSEIRSRAVSDSDFAISVTKSAHAKCERC
                     WNYRKAVGQDASHPTLCDRCVEAVQ"
     gene            complement(4483..>5091)
                     /gene="nadA"
                     /locus_tag="VLL06_11220"
     CDS             complement(4483..>5091)
                     /gene="nadA"
                     /locus_tag="VLL06_11220"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HSS31579.1"
                     /translation="VIEWSWARREKILFFPDEHLGRNTANKMGLPREAMIVWDPYQPN
                     GGNTREAIQRAKLILWKGHCSVHQMFQPVHVDNFRKRYPDGKVIVHPECHEDVVNRAD
                     LSGSTELIIRTVTAAPPGTAWAVGTELNLVNRLKRDLTDKTVLFLSSTVCQCATMFRI
                     DGAHLCWAMENLADGHVVNHIVVPDDEKYWARIALDRMMSVS"
BASE COUNT         1147 a         1515 c         1345 g         1084 t
ORIGIN      
        1 gaccaccgat accgtcggcg tgctctgccc ggggaagggg agcctcggct cggggtgttg
       61 tcgctcgatc gtgctcatac gcagctgcgc cgcaaaagca ggcgtccctg tactggaacg
      121 ctccttattc gtcgcaccgt gcgccccaga gggtacgcct ccggtttcga ctcgcctgcg
      181 gccttgctgg acaaactttt tgagcatcct gtatggtata ccctggttgt tccaggcgtg
      241 cagatcaacg agattcaggc gcgcccacag agtgtttcca caacttgcta ggaggatttt
      301 tcgcgatggc tcaatcccag tccgatacgg tacatgtctt tgacacgtgg gtcagaggcg
      361 caaaaggact actgcatttc gacgtgatga cgactgatga ggcaacggca ttgaggctcg
      421 ccaagcaaca tttggctagc cttggagaag gtgccgtgcc ggtgacggtg aaagaatgtc
      481 agttctgcca cacggaaccg ctcgtgatgt ttaactcaga gcagcagcgc caattccgcg
      541 agcagggcgg gttcatcatc accctctcgg cctagcaggt taagggaaat cgatgtcggc
      601 tcgctgggac tccgctggct tgcctgtcct tggcaacaga acacgacccc gcaggaggct
      661 caaaaaggcc agctttctta cccgcccaac cccggcgcgc caagacgcgc tgttccgcga
      721 gcaaggccgc agcagttttt cacactattt ctaacgggtg gcttggtcga tcctcgattg
      781 cgcgcgtcga acgagcacct tccgagcgtg cgcgttccgc gagcaggagg acgaccgggc
      841 tacccgttcc tccctttttc agcatcctgc taagaagcag gtggaagctc ttgaggccgg
      901 agcggcacgt tcttcttctc aaaagcgaaa tggatgatca agaagacgac ccccacactg
      961 atcgccgagt cggcgacatt gaaggccggc caatggtagg agtccacata gacatcgaga
     1021 aaatcgatca cctcgccata ccgtaatcga tcgatcagat tcccaatcgc ccctccaaga
     1081 atcccggcaa tactgagctg ccccacccaa tctttctccg gtaaccggta gagaatcgtt
     1141 cccagtaagg ccagcgcaaa caacgacgtg acgccgaaga acaccatgcg aaatgcatcg
     1201 ctgctgccgg ccagcaaccc aaaggccgca ccgggattgc ggatataggt caggctgaag
     1261 agattgggaa caaccgggat agattcattc aggtgcatcg cctccacgat ggacagtttc
     1321 gtcgcctgat cgatcacgat gatcgtgcca ctgagcagcg ccaaaagcag atagcgaaga
     1381 ggccttccca tcactgcacc gcctccacgc aacggtcgca caaggttgga tgactggcat
     1441 cttgccccac tgccttgcga tagttccagc aacgctcgca cttggcgtgc gccgacttgg
     1501 ttacggagat tgcgaagtcg gaatcggaca ccgcacggga ccgaatttcc gacgtcacct
     1561 ccgacacaat gaacagcgta gctaagtccc tgcacgcgct tgttatccac atgccagtct
     1621 ttgcatccac cgtaagatgg acttgcgctt caagcgaaga tccaatgacc ttctcccgcc
     1681 gtctctcctc caacacccct tgaacctggc tacggtattt caacagccgc tcccatctct
     1741 cagctaaggc ggcatcctgc cactgagact gaatctctgg aaatgtactc agatggacac
     1801 tgctcactcc tagatgtcct cccggctgcg cagccaaggt ccgccagatc tcctcggccg
     1861 taaaactcaa caccggagcc atcagcttcg tcatggcgac gacgatatcg aagagcaccg
     1921 tctgcgaccc gcgacgcaag ggcgaatcgg cacggaatgt gtagagccga tctttgagaa
     1981 tatccagata gaccgcgctc aggtccaccg agcagaagtt gttaagggca tggaagatcg
     2041 tatgaaactc aaaatcttca taggatttct gaacccgcgg aatcagttcg ctcaaccgat
     2101 gcagcgccca acgatctaat tcaggcaatt gctcatacgg gattcggtcc ttgtccgggt
     2161 cgaagtcgta tagattactc aggagaaagc gacaggtatt ccgaatcttt ctatatgcct
     2221 cgatgagatg ggtcaagatt tcaggagaaa ttcgaacatc atcgcgataa tcctgtgccg
     2281 aaacccatag acgtaaaatt tctgcgcccg attgtttaat cacgtcctgc ggcgcaacga
     2341 cgttgccggc cgacttggac atcttcttac cctgcccatc aaggacaaaa ccgtgggtca
     2401 ggaccgcctt gtagggtgcg cgatggtcgg tgacgacacc ggcgagcaat gcactgtgaa
     2461 accaaccccg atgctgatct gacccttcga gatagagatc ggccggccac cacttccgtt
     2521 ctttcaatac cgcggcataa ctgacgcccg actcgaacca cacatcgagg atgtctcgct
     2581 ctttttcgaa tgccgtcccg ttgcagttcg aacagctcgt gcccttgggc aggagatctg
     2641 cagccgattg ctcgaaccac acatcggccc ctttggactc catcaacgtg gctatgtgct
     2701 cgatcacgtt cggatcagcc aggacagcac gacacccgat gcaggtgaaa ccgggaatcg
     2761 gcacgcccca cacgcgctga cgagacaaac accaatcggg acggttctcg atcattccgt
     2821 tgatccgatc acggccgtag gctggaatcc attgaacccg gtcgatctcg gccagagcct
     2881 cttttcgaag atcgttgatc tccatcgaca cgaaccactg ttcggtggcg cggaagatga
     2941 caggattctt gcaacgccaa cagtgcggat aagagtggtt caacgaccca tggcccagta
     3001 agcgtccatt agccttcagg aactccacaa tttttggatt cgccttcagt acatgctggc
     3061 cagcaaactc tttcaccacg tcagtaaacc gcccggcatt gtcgaccggc gccagaattt
     3121 ccagccgttc gcctggcgtg gccttggaat tgtgctcaat gacaaggatg tagtcttcca
     3181 tgccgtgacc cggagcaata tggacacaac cggttccctg atcgatggtc acgaagtcgc
     3241 cgagcaaaat cggcgagagg cctgtggtca ggggccgttg cgtctcgatc ccttcgaagc
     3301 cttcttttcc cttcttcacc ccgaacacgc gagccccttc gagactgcag gccttcacca
     3361 cgctctccac caatttctcc gccacgatca ggacctcgtc gccgacctgg acaaacgcat
     3421 agtcgatatc cccatgcaaa cagaccgctt gattggctgg aagtgtccag ggggtcgttg
     3481 tccagatgac gacagagact aacttcatac cgccggggaa cacgacaccg ggaaatgtct
     3541 ggctcagtac cgtaggggac gtaaccagcg ggaacttcac atagatcgag ggagaggtat
     3601 ggtcttcata ctcgacttcc gcttcggcaa gggcagtttg gtccaccgtg caccaaagca
     3661 ccggcttcag tcccttatag acgcctcccc gttctacgaa tttcccgaac tcgcggatga
     3721 tcgtggcctc atagcctgga ttcattgtga ggtagggatg ctcccaatcg cccaagatgc
     3781 cgagccgttg aaactcttca cgctgaatga tgaaaaattt ctccgcatat tcccggcaga
     3841 gccggcgaat ggccggggta tcgagcgtct ttttcttgtc gccgagttct ttgagcacct
     3901 gatgctcgat cggtaggcca tggcaatccc atccgggcac atagggcacc tggtagcctg
     3961 ccatggtctt cgatttgatg atgatatctt tgaggatctt gttcagcgca tggccgatat
     4021 gaatgcgccc attcgcgtaa ggcggaccat cgtgcaagat gtaccggggc cggccttttc
     4081 ccgcctcctg aatctgctgg tacaacccct cccgctccca cgaagctaga agttcaggct
     4141 cccgcaacgg caagttcgcc ttcatcggga aatcggtctt ggggagattg agtgtcgcct
     4201 tgtagtccat aacctatctg gtcttccgcg aaacccttag aatgtgatgg ggatcaagaa
     4261 ttggaatata ccggaatgac aggcgagtca ccagtcctta ccttgaatgg cggggtcgtc
     4321 agaaacgtct tccggcaagg ccgcagcgag cgagagcccg agacatacgg ttctaggtac
     4381 gttgagggtt tgagcgaagt gagaacgaag cccggtgaaa gatgcgtctc ggcgcatcgg
     4441 ggtgggcggg tggaatcaag tcgctttttc accatcctgg ggctaactga cggacatcat
     4501 gcggtccagt gcgatcctgg cccaatactt ctcatcatcc ggaacgacga tatggttcac
     4561 gacgtggccg tcagcaaggt tctccatcgc ccaacagaga tgggctccat cgatccggaa
     4621 catcgtcgcg cactggcaga ccgtggatga caaaaacaga actgtcttgt cggtgagatc
     4681 acgcttcagt cgattgacca gattcaattc tgttcccacc gcccaggccg tccctggcgg
     4741 cgctgccgtc accgtacgga taatcaactc tgtggaccct gacaggtccg ctcgattgac
     4801 gacatcctca tgacattccg gatggacgat caccttccca tccggatacc gcttgcggaa
     4861 attgtctacg tgcacgggct ggaacatctg gtgcacactg cagtggccct tccagagaat
     4921 gagtttcgcc cgttggatcg cttcccgcgt attgcctccg ttgggttgat agggatccca
     4981 gacgatcatg gcctctctcg ggagccccat cttgttggcc gtattacggc ccaagtgctc
     5041 atccgggaaa aagaggatct tctcccgcct ggcccaagac cattcaatca c
//
LOCUS       DATIPF010000454         4265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1763533, whole genome shotgun sequence.
ACCESSION   DATIPF010000454 DATIPF010000000
VERSION     DATIPF010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763533"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VLL06_11225"
     CDS             complement(<1..495)
                     /locus_tag="VLL06_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSS31580.1"
                     /translation="MYRKLVLDNGVRVVTERMPTLKSVTVGVWVNIGSRDEQPSQAGY
                     SHFIEHMLFKGTRKRSSAEISREIDALGREMNAFTSRETTTYYVKVLDQQLERAVELL
                     SDLFHHSRFAPKEVEKEKQVVLEEIRMVRDDPEDLVQELHSGQVLGRHPLGRPILGRE
                     KTIRG"
     gene            complement(501..2618)
                     /gene="pnp"
                     /locus_tag="VLL06_11230"
     CDS             complement(501..2618)
                     /gene="pnp"
                     /locus_tag="VLL06_11230"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HSS31581.1"
                     /translation="MVHTVEIEVAGRTLRLETGRVARQADGSIWASYGDTVVLATAVA
                     SQIAKPGTDFLPLTVDYQEKSFAAGKIPGGYFKREARPSEKAVLTSRQIDRGLRPLFP
                     EGYYFETQVIASVLSADQTGSSDILAIIASSAALTISNIPFDNPIAGIRIGRLGGKFV
                     VNPDLETAAKSELQLVVAGTADAVMMVEAGANELSEAIMLEAIELAHAEIKKIVGKIR
                     ELQALAGKPKRKVEKEQIDSALAAQVKSLVAQGIREAIMIPNKAARQERLDEVKNSAV
                     QTLKSVDDPNRERHVKLVFHDLEYTEVRNMILEKGSRADGRGPADIRPITCEVSTLPR
                     THGSAIFTRGETQSLAVVTLGTTDDEQRIDALEGEYMRTFMLHYNFPPFSVGEARPLR
                     SPGRREVGHGALAERALASVIPSKEAFPYTLRIVSDILESNGSSSMATVCGGSLALMD
                     AGVPIREAVAGIAMGLIKEGDRVMILSDILGLEDHLGDMDFKVCGTKQGVTALQMDIK
                     IGGITSALMQRALEQAKAGRLHILSCMEKALQAPRTTLSAYAPRIFTMKVKQDKIREI
                     IGPGGKTIRGIIADCGVKINVDDSGTVTIASVDGASAEKAKEMISRITEEVEIGKIYM
                     GTVRKLMDFGAFVEVLPGTDGLVHISQLAHHRVQAVSDEVKEGDQILVKVLEVDRQGK
                     IRLSRKEAMPAPTGAGATDPSAR"
     gene            complement(2764..3033)
                     /gene="rpsO"
                     /locus_tag="VLL06_11235"
     CDS             complement(2764..3033)
                     /gene="rpsO"
                     /locus_tag="VLL06_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HSS31582.1"
                     /translation="MALLKEAKTELIKQYRQHETDSGSPEVQIAVLTNRITYLTEHFK
                     LHKKDHHSRRGLLLLVGRRRRLLDYLRGVSDARYRAVIERLGIRK"
     gene            complement(3078..4004)
                     /gene="truB"
                     /locus_tag="VLL06_11240"
     CDS             complement(3078..4004)
                     /gene="truB"
                     /locus_tag="VLL06_11240"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HSS31583.1"
                     /translation="MDGVLTIKKEAGWTSHDVVAKVRHLLGGVKVGHAGTLDPAATGV
                     LPVLIGRGTRIAEYLVEWDKEYRAVLRLGETTDTQDATGTVLANKVSDQVTSEAIHEV
                     VDRFRGPIEQVPPMYSAVKVGGVPLYKSARAGKTIARNARTIVIHTLEVLAIQGRDVA
                     LRIVCSKGTYVRTLCADIGEALGVGAHMLALERRRVGPLTIDHALTVDEVVTRHALGR
                     LGDDLLSLDHALVRLGIAVVDAQTADRARHGAPVPAAKILRWERMDDGGTGVHRPVRI
                     HDTEGRLVAIGTCPEKSDGAIKVEKVLIDQDV"
     gene            complement(3989..>4265)
                     /locus_tag="VLL06_11245"
     CDS             complement(3989..>4265)
                     /locus_tag="VLL06_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31584.1"
                     /translation="PGASLAAAPLAAFPALVPGLLSAMIAPGGGQLVASSCRAEHHTA
                     TVRVVELSAANGRLIRVLRTQAARFGNDADVQDAIFSTCQVPSWTAF"
BASE COUNT          847 a         1349 c         1137 g          932 t
ORIGIN      
        1 ccccctgatc gtcttctccc gacccaggat agggcggccg agcggatggc gtcccagcac
       61 ttggcccgag tgaagctctt gcacgagatc ttccggatcg tcccggacca tacgaatttc
      121 ttccaggacg acctgttttt ctttttcgac ttctttgggg gcgaatcgtg aatgatggaa
      181 cagatcggag aggagttcga ctgctcgctc aagttgctga tccaacacct tcacataata
      241 ggttgtcgtt tcacgcgacg tgaaggcatt catctctcga ccaagggcgt cgatttcgcg
      301 agaaatttcg gcggaggatc gcttgcgcgt tcccttgaaa agcatgtgtt cgatgaagtg
      361 ggaatagcct gcctgggatg gctgttcgtc ccgtgacccg atgttaaccc acacaccgac
      421 ggttaccgac ttgagcgtcg gcatccgttc agtcacgacc cgcaccccat tgtccaggac
      481 gagtttgcga tacaagacag ttacctggcc gacggatccg tcgcaccagc cccggttgga
      541 gccggcatag cttccttgcg gctgaggcgg atctttcctt gccgatcaac ttctagaacc
      601 ttcaccagga tttgatcgcc ttccttcacc tcgtcggaca ccgcttggac tcgatgatgc
      661 gccagctgcg agatgtggac cagcccatcg gttcccggca ggacctcgac aaacgcgccg
      721 aagtccatga gcttccgaac cgtgcccata tagatcttcc cgatctctac ctcttcggta
      781 attcgactga tcatctcttt ggccttctcg gccgaggctc cgtcaaccga cgcgatcgtg
      841 acggtaccgc tatcgtcgac attaatcttg accccgcaat ccgcaatgat gccgcggatc
      901 gtcttaccac ccggaccgat gatctcgcgg attttgtcct gcttcacttt catcgtgaag
      961 atccgtggcg cataggccga cagagtggtt cttggcgctt ggagcgcctt ctccatgcag
     1021 ctcaaaatgt gcaatcggcc tgctttggcc tgttccaagg cccgctgcat caaagccgac
     1081 gtgatgccac cgatcttgat atccatctgc aatgccgtga caccttgttt ggtgccgcag
     1141 accttaaagt ccatgtcgcc caagtgatcc tccagcccga gaatatcgga aaggatcatg
     1201 acccgatcgc cttccttgat cagtcccatc gcgatcccgg ccacggcttc tctgatcggc
     1261 acgccggcat ccatcagggc aagactcccg ccgcagacgg ttgccatcga agaggatcca
     1321 ttagattcga ggatatcgga cacaatgcga agggtgtagg gaaaggcttc cttgctggga
     1381 attactgagg ccaacgcccg ttctgccaac gcaccatgac cgacttcccg gcgccccggt
     1441 gagcgaagcg gtctcgcttc gccgacactg aacggcggga aattgtaatg gagcatgaac
     1501 gtccgcatgt actccccttc caaggcatcg atcctctgct cgtcgtccgt ggtccccaac
     1561 gtgaccacgg ctaaactctg ggtctcgccc ctggtgaaaa tcgcggaacc gtgagtccgt
     1621 ggcagcgtgc tgacttcgca ggtgatcgga cgaatatccg ccggaccccg tccatccgcc
     1681 cgcgaccctt tctccagaat catgttgcgg acttccgtat attccaagtc atggaatacc
     1741 aacttcacat gccgctcccg gttcggatcg tccacgctct tgagcgtttg aacggcgcta
     1801 ttcttgacct cgtccaatcg ttcctgcctg gccgctttgt ttggaatcat gatggcctcg
     1861 cggatccctt gggccaccag ggacttcact tgagccgcga gcgcagagtc gatctgttcc
     1921 ttctccacct tccgcttggg tttgccggca agggcctgta gctcgcgaat tttgcccaca
     1981 attttcttaa tctcggcatg ggcgagttcg atcgcttcaa gcatgatggc ttcggacagt
     2041 tcgttcgccc ctgcttcgac catcatgacc gcatcggccg tccccgccac cacgagttgc
     2101 agctcgctct ttgctgccgt ctcaaggtcc ggattgacga cgaacttgcc tcctagccga
     2161 ccgatcctta taccggcaat aggattgtcg aacggaatat tcgagatcgt gagggcagca
     2221 gaggaagcaa taatcgcgag gatgtcggac gaaccggtct gatcggccga gaggactgag
     2281 gcgatgactt gtgtctcgaa ataataacct tcgggaaaaa gcgggcggag tccccgatcg
     2341 atctgccggc tggtcagcac agctttttca gatgggcgtg cttcccgttt gaaatacccg
     2401 ccgggaatct tccccgctgc gaaggatttc tcctgataat cgaccgtgag aggaagaaaa
     2461 tctgtgcctg gcttggcgat ctgtgacgcg acggctgtgg ccagtacgac ggtatcgcca
     2521 tacgacgccc aaatcgagcc gtctgcttgt cgtgccacac gaccggtctc gagtcgcagg
     2581 gtgcgtccgg caacctctat ttcaactgtg tgtaccatct gagactcctc tggttaggcc
     2641 ccacagatgc ccacagagag agactcgagc gattgtggga cactccagcc tctgtcagtg
     2701 ttcacctgtg cgactgatga ctgctcgctg gtgcactccc tggcgcgaca gcgagacgga
     2761 aaactacttt ctaatgccta atcgctcgat caccgcacga taccgtgcat cgctgacgcc
     2821 acgaagatag tcaagcaaac gccggcgacg tccgaccaag agaagcaggc cacgtcggga
     2881 atgatgatct ttcttgtgga gcttgaaatg ttccgtcaaa tacgtgatcc ggttggtcaa
     2941 cactgcgatc tggacttccg gcgatccgga atctgtttca tgctgccgat actgcttgat
     3001 caactcggtc tttgcttcct tcagtaatgc catgggcttc ttccttccta ttcctttcct
     3061 tcatccgcaa cgacgaatta tacgtcttga tcgatcaaca ccttctcgac cttgatcgcg
     3121 ccatccgact tctcaggaca tgtcccgatc gccaccaaac gtccttcggt atcgtggata
     3181 cggacgggcc ggtgcacccc ggtcccgcca tcatccatcc tctcccatcg aaggatcttg
     3241 gccgcaggca ccggagcccc gtgtcgcgca cggtcagcgg tctgtgcatc caccaccgcg
     3301 atccccagtc ggaccaatgc atgatccagc gacaacagat cgtcacctaa ccgaccgagg
     3361 gcatgacgag tgaccacttc atcgaccgtc agcgcgtgat cgatcgtcaa gggacccact
     3421 cgccttcgct ccaacgccaa catgtgggca ccaacaccca acgcctctcc aatgtcggcg
     3481 cagagtgtcc gcacgtaggt ccctttggag cagacgatgc gcaatgccac atcccgcccc
     3541 tgaattgcca gcacctcaag tgtatggatg acgatggttc gcgcatttcg cgcaatcgtt
     3601 ttaccagctc gggcagactt atacagaggg acacccccga ctttcaccgc cgaatacatc
     3661 ggtgggactt gctcaatagg cccgcgaaat cgatcgacca cctcatgaat cgcctctgat
     3721 gtcacctgat cgctcacctt gttcgccagc accgttcccg tcgcatcctg cgtatcggtg
     3781 gtttctccga ggcgcagcac ggcacgatat tccttatccc actcaacaag atattcggca
     3841 atacgggttc cccgcccaat cagcacaggc aagactcccg ttgcagcagg gtcgagggtg
     3901 ccggcatgac cgaccttgac tccgccgaga agatgccgca ctttcgccac gacatcgtga
     3961 gaggtccagc cggcttcctt cttgatggtt agaacgccgt ccacgacggc acctggcacg
     4021 tcgagaagat cgcgtcctgg acgtcggcgt cattgccgaa ccgggccgcc tgcgtccgca
     4081 gtacccggat cagccgcccg ttcgcggcgg acagctcgac gacccggaca gtcgcggtgt
     4141 ggtgctcggc gcggcaggac gacgcgacga gctggccgcc gcctggtgcg atcatcgcgg
     4201 acagcaggcc cggcacgagg gcggggaagg cggccagcgg tgccgcggcc aggctggcgc
     4261 cgggc
//
LOCUS       DATIPF010000455         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1057803, whole genome shotgun sequence.
ACCESSION   DATIPF010000455 DATIPF010000000
VERSION     DATIPF010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057803"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VLL06_11250"
     CDS             complement(<1..259)
                     /locus_tag="VLL06_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSS31585.1"
                     /translation="MMRLDGKIILLTGSRGGLGSVVIPILSGAGATLIVVDRVLSQDI
                     IDRRGGFRADVTNESEVTRVVADTVRQAGRIDGLINLVGAFV"
     gene            complement(279..1025)
                     /locus_tag="VLL06_11255"
     CDS             complement(279..1025)
                     /locus_tag="VLL06_11255"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HSS31586.1"
                     /translation="MSLQQALRSHIDIRRSHQRFHTQISWLDSHHSFSFADHYDPQNT
                     HHGLLLVSNDDVVRPEMGFRTHPHHDMEIVTWVLEGELEHKDSEGNVGILYPGLAQRM
                     SAGTGIRHSEMNPMDDRDVHFIQMWVPPDTERINPSYEQLDINGALERGGLIPIASGR
                     GHEAAISIKQRGAVLWGGRLRPGESVQIPDAPYVHLFIAKGGAELEGADVLHHGDAVR
                     LVAAGARRLTADAGTGAEVLIWETDQDLEA"
     gene            complement(1110..2117)
                     /locus_tag="VLL06_11260"
     CDS             complement(1110..2117)
                     /locus_tag="VLL06_11260"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HSS31587.1"
                     /translation="MWKSLAVMPIVFSLAVPAVAVAQEAPASEKSQSEQTVDALNGVF
                     GVHPGARAAHAKGIVLEGTITPSASAAAVTKAAHLQKKKAAIPVTVRFSDGPGVPTIP
                     DTEAGAGPRGMAVKFHLPDKSVTDIVAHSFNGFPVATGAEVRDLFLAIAASKPDSPKP
                     TALDTFLSTHPATKAFVEAPKPAPVSFGTLAYFGVNAFKFTNAKGVATFGRYQLQPVG
                     GEKVLTSEQVSKKGPDYLAEEIRDRVRRASVKFKLLLQVAGPDDKIDDPTMAWPDSRK
                     KVELGSLVITKAVKDSPAAEKQLLFMPGALVPGIEAADPMIADRSAAYIVSLSRRAKT
                     Q"
     gene            complement(2200..>2576)
                     /locus_tag="VLL06_11265"
     CDS             complement(2200..>2576)
                     /locus_tag="VLL06_11265"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSS31588.1"
                     /translation="AQEAARHMGDGGRIITIGSVNADRMPFEGGGVYAMTKAAVAGLT
                     RGLARDLGPRGITVNNVQPGPVDTDMNPANGPFAETMKSFMALKRYGQGAEIAGLVSY
                     LAGPEAGFITGASLTIDGGFSA"
BASE COUNT          523 a          793 c          705 g          555 t
ORIGIN      
        1 gtacaaatgc tcccacgaga ttgatcagac catcgattcg tccggcttgt ctcaccgtgt
       61 ccgccacgac tcgcgtcacc tcgctctcat tcgtgacatc ggccctaaat ccaccccgtc
      121 gatcaatgat gtcttgggac agcacccgat ccactacaat gagcgtcgcg cccgctccag
      181 agagaattgg gatcacaaca gaacccagtc ctcccctgga cccggtgaga agaatgattt
      241 ttccatccag ccgcatcatc cgctattgcc ttctttcctt aggcttccag gtcttgatcg
      301 gtttcccata tgagcacctc cgctcccgtg ccggcatcag ccgtgagtcg ccgcgcaccg
      361 gctgccacga gacgaacggc atcgccgtga tgaagcacat cagcaccttc gagttcggca
      421 ccccccttgg caatgaagag atgcacgtac ggggcatcgg gaatctgcac cgattccccg
      481 ggcctcagcc gtccacccca cagcaccgca cctcgctgtt tgatggaaat cgctgcctca
      541 tggccgcgcc ctgaggcaat gggaatcaag ccgccccgtt ccagcgctcc gttaatatcc
      601 aattgttcat agctcggatt gatgcgttcc gtatccggcg gaacccacat ctgaataaag
      661 tgcacatccc tgtcatccat cgggttcatt tccgagtgcc ggatcccggt accggcactc
      721 atacgctggg ccaacccagg atagagaatg ccgacgttgc cctccgaatc tttatgttcc
      781 agttcaccct cgagtaccca ggtcacaatc tccatatcgt gatggggatg cgtgcggaac
      841 cccatttcgg gtctcaccac gtcatcatta ctcaccagaa gcagtccatg atgggtgttc
      901 tgaggatcat agtggtctgc aaagctgaag ctatgatggg aatcgagcca actgatctgt
      961 gtgtgaaacc gctgatgact ccggcgaata tcgatgtggg atcgaagggc ctgttgtagg
     1021 ctcatgatgt gttcctccca tggtcacgca aatccaaagc gaggaaacca atgggcaggg
     1081 ggaaccccct gcccatcctg ctgacctgtt tactgtgtct tcgcccgccg agataacgac
     1141 acgatgtagg ccgccgaacg atcggcaatc atcggatcgg ccgcttcaat gcctggaacc
     1201 agcgcccccg gcatgaagag caactgcttc tctgcggcag gactgtcttt cacagctttg
     1261 gtaatcacaa ggctgcccaa ttcgactttt tttcgggaat ccggccaggc catcgtggga
     1321 tcgtcgatct tgtcgtccgg tcctgccacc tgcaggagca atttgaactt gactgaggcg
     1381 cgacgaaccc ggtcgcgaat ctcctcggcc agataatcag gtcccttctt cgacacctgt
     1441 tcactggtga gaaccttctc tccaccgacc ggctgaagtt gatagcgccc gaacgtggca
     1501 actcccttcg cattcgtgaa cttaaacgcg ttcacgccga aataggccag agtcccaaaa
     1561 ctcaccgggg ccggcttcgg agcttctacg aatgccttcg tggcgggatg cgtgctcagg
     1621 aacgtgtcga gtgcagtcgg tttggggctg tcgggtttgc ttgcagcgat cgccagaaat
     1681 aggtctctca cttcagcacc cgtcgcgacg gggaatccat taaatgaatg ggcgacaatg
     1741 tctgtgaccg acttgtcggg gaggtgaaat tttaccgcca tgccgcgagg acctgccccc
     1801 gcttcggtgt ccggaatcgt tggcactccc gggccatcag agaaccgaac ggtcacggga
     1861 attgcagcct ttttcttttg aagatgggcg gctttggtca ccgccgcggc tgaagcgctt
     1921 ggagtgatcg ttccttccag gacgatgcct ttcgcgtgcg cagcgcgggc tccaggatga
     1981 acaccgaaca ctccgttcaa ggcgtccaca gtctgttcag actgtgattt ctcgctggcc
     2041 ggcgcctctt gagcgaccgc gactgcggga acggcgagag agaaaactat cggcatgact
     2101 gcaagtgatt tccacacgga gtgctccttt tgttagagtg gctcccttca aagagggctg
     2161 agaaacggga agctacgcct ttgcggcccc ccaggtgtat caagcggaga atccgccgtc
     2221 aatcgtcaga ctcgcaccag tgataaatcc ggcctctgga ccggcgaggt aggagaccaa
     2281 ccccgcaatt tcggctccct ggccgtaacg tttgagggcc atgaagcttt tcatcgtctc
     2341 ggcaaagggt ccgttcgcag ggttcatgtc agtatccacc ggacctggct ggacgttgtt
     2401 gaccgtgatg cctcgagggc cgaggtcacg ggcgagtcca cgggtgagac cggcgaccgc
     2461 cgccttggtc atcgcgtaca caccaccgcc ctcgaagggc atgcggtcgg cgttgacgct
     2521 gccgatggta atgatgcgcc cgccatcgcc catgtgacgc gccgcctcct gcgcgg
//
LOCUS       DATIPF010000456         9291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_529588, whole genome shotgun sequence.
ACCESSION   DATIPF010000456 DATIPF010000000
VERSION     DATIPF010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9291
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529588"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..407)
                     /locus_tag="VLL06_11270"
     CDS             complement(189..407)
                     /locus_tag="VLL06_11270"
                     /inference="COORDINATES: protein motif:HMM:NF044585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31589.1"
                     /translation="MPEGKKVRIRVRTATYVYVGDFLIPPMRNRVSDAINEEQRLFIS
                     LTDVLINEKERSDFVAINKNLIESIAQL"
     gene            718..1227
                     /locus_tag="VLL06_11275"
     CDS             718..1227
                     /locus_tag="VLL06_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31590.1"
                     /translation="MILPYKLPLFPLVVLLSSIFLISVGCGGKKIQYPDDHERYLRID
                     RAVESLRQAYVKKDSSDLASLMIPSDQLDRLREEAQGDFETFSAITLDFAVERIMVEG
                     DNVDVFVHWQGLWKKGAEDPGLRQRGHTRLQWVGTQSILLRGVQGDSPFGMKARQAMV
                     DPTRPPPKK"
     gene            1231..2889
                     /gene="nadB"
                     /locus_tag="VLL06_11280"
     CDS             1231..2889
                     /gene="nadB"
                     /locus_tag="VLL06_11280"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HSS31591.1"
                     /translation="MPSRRSHAPVQADFLVIGSGVAGLRAALELSRAGRVMVLTKGHP
                     LQSSSIHAQGGVAVAMSEEDDVAIHLTDTLKAGHGLCRKQAVRVLVEEGPERIQELIG
                     WGAKFDKVGGKFAVAREAAHSRSRILRARGDATGNEMVRVLIAEVNRQERIQRLDHHF
                     TVDLVVDAGNCRGAVVLDEGSGRQFVLPARAVVLTTGGAGQIYARTTNPPNATGDGMA
                     MALRAGAVLQDMEFVQFHPTALYLPSSPPFLLSEAMRGEGAQLRNNKGALFMQRYHPM
                     GALAPRDIVSRAILAEMAATKARHVYLDVTHLGAEFIRQRFPTIYATCLRYDIDITEE
                     WIPVSPSAHYMMGGVWTDLNGATTVPGLFAAGEVACSGVHGANRLASNSLLEGLVFGM
                     RAASAAVAFADQQKLPALSSQETTLRTGQFGTLEDAEKLRSSLRRTMWGQVGVIRSGE
                     SLIRACAQLSRWAQLVTQPFASRSALEVKNMVQVAQCVAEAALWRENSVGAHYRSDFP
                     EAELAGWRQHSRLSSGEVVSGKISQKSRGLLLSPREHIGRLDAP"
     gene            complement(2810..3520)
                     /locus_tag="VLL06_11285"
     CDS             complement(2810..3520)
                     /locus_tag="VLL06_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HSS31592.1"
                     /translation="MHNTISLVLTSLRRQAAIGLTTTVITASGSLTFSVPATTAEIYQ
                     FIGHDGSISLTNVPSDSRYRKVEIESSRFHSILSERELEPVIRRHSSQHQLHPALIRA
                     VIKAESDFDPRAVSRAGAIGLMQLMPQTAVRLDVRDMYDPDDNVGGGTKYLRQLLDRF
                     HGNLPLALAAYNAGENVVDRYQALPPFDETRQYVRKVLRYYRTFLVRDGVITERPVGR
                     YAPAETTATPAISEISSH"
     gene            complement(3634..4482)
                     /locus_tag="VLL06_11290"
     CDS             complement(3634..4482)
                     /locus_tag="VLL06_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HSS31593.1"
                     /translation="MKEEEAVVSVFDHGFLYGDGVYETIRSYGNRIFMRDLHLARLHR
                     SADAIGLTIPISEHRWPLLLHEAMTRNDVGHERTDAYLRITISRGTGDIGLDPALCPT
                     PTVVIMTKPLHPPPPEQYHAGVKLIVARTRRNLPSALSPQIKATNFLNNILAKREAIA
                     ADAFDSILLNWESHLTECTVSNLFFVQAGRLCTPALACGLLDGITRDIVIGLAQEKQI
                     PVEEGHFGIEAIHKADECFITNTSLEVMPVTMVNGQPVGNGTPGLLTRQLHTYFIANK
                     ARFLEP"
     gene            complement(4512..5936)
                     /locus_tag="VLL06_11295"
     CDS             complement(4512..5936)
                     /locus_tag="VLL06_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HSS31594.1"
                     /translation="MNGQKRTPFELYRLIASSSRPSFLLDSGKGTDGGSRYSFLGSNP
                     FSVLTGRQGQASIRTHDGREYLSNDPFGSLCRLFAGPQIEFPPGLPNFCGGAVGYLSY
                     DLVREFETLPSIAKDDLLLPHLQFGLYDIVTAVDHQTGLVKILFCPPMERFVGEPREK
                     LYREGLDRLAEWEVRLSGRPSPLDPLPSLDQMAFHPDQIRDAYLQRVRRCQEYIAAGD
                     IYQANLSHRFTLKPLSSYDEGTDRQLHEQELYRRLQAVNPSPFSGLIHFDDVTLISSS
                     PERLIRLRNRQADTRPIAGTRPRGVDTHDDQRLVGELLANEKERAEHLMLVDLERNDL
                     GRVCRFGSVQVDELMAIEQYSHVSHIVSNVSGGLRPDATPFDLIQALFPGGTITGVPK
                     IRCMEIIEELEPVRRGPYTGSFGYLGWNGDLDLNIVIRTLIWCGGKGYLQVGAGIVAD
                     SDPAKEYEETLQKAQAFFSALQHS"
     gene            6174..6395
                     /locus_tag="VLL06_11300"
     CDS             6174..6395
                     /locus_tag="VLL06_11300"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HSS31595.1"
                     /translation="MPPSQDPLYTGLGQAVRIATDLLAALIVGGAVGWACDTYLFDST
                     PWAMVGGLVLGIVAGIRNAYRSAQRWPKT"
     gene            6427..7176
                     /locus_tag="VLL06_11305"
     CDS             6427..7176
                     /locus_tag="VLL06_11305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HSS31596.1"
                     /translation="MEESPLHQFELQNWIPISLGGLDVSINKAVVFMWVVVSLAAVLM
                     VMAGSARKLVPGKLQSLAEMMVDFIRSMIMDTMGKDGMRFFPLIATLFLFILFSNLIG
                     LIPGTYTVTSQIIVTAVFSCMVYGLSLVIGFWIHGIKFLGILIPPGTPGWLLPLMIPI
                     ELISQLARPISLAVRLFANMTAGHVMLAVLFSLTIGGGLLIGWLPFAFTVAIYGLEFG
                     IAFIQAYIFSILTCVYLGDAFHLHGHDEHAH"
     gene            7213..7443
                     /gene="atpE"
                     /locus_tag="VLL06_11310"
     CDS             7213..7443
                     /gene="atpE"
                     /locus_tag="VLL06_11310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HSS31597.1"
                     /translation="MDSAAAGLIGMGCAAAGFAGAGVGIGYIFGKMIEVVARQPEAEA
                     RVTKYMWIGFALVEAIALYGLVIAFIIMGFRK"
     gene            7519..8025
                     /gene="atpF"
                     /locus_tag="VLL06_11315"
     CDS             7519..8025
                     /gene="atpF"
                     /locus_tag="VLL06_11315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF017242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HSS31598.1"
                     /translation="MPQFEPDFFSSLIFWEVISFAILLFVLYKYAFPGILGALEEREK
                     RIKDSLDQAEHHRSEAERKLKEYEAKLNSAAKDAEGILAAAKERVQRLMEENEQRMTT
                     EAERIKGDATREIDHERRKAIQDIRSHTTDLALMVAEKVVQRSLNEADHRKFADEALE
                     ALSKSYQR"
     gene            complement(8044..8853)
                     /gene="larE"
                     /locus_tag="VLL06_11320"
     CDS             complement(8044..8853)
                     /gene="larE"
                     /locus_tag="VLL06_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HSS31599.1"
                     /translation="MTAESLQGKLTTLRQVISDMQSVLVAFSGGIDSTIVLKIAHEQL
                     GEHALAVTAVSPTFPTIELDGAKQVALEIGARHELVHTDQLDVPAFVQNDATRCFHCK
                     TDLYQLLDKLREPRASRWIVDGTNLDDLGDDRPGIKAAREWGVRSPLVEASLSKSDVR
                     ALAQTLGLSNWDKPAAACLSSRIPRGTPITIDSLRRVEQAEEILQAEGFRHVRVREHG
                     EVARIEVGAEEFSLLNQPDVRARISAHLRKAGFRFVCVDLEGYRPGGVSLG"
     gene            complement(8888..>9291)
                     /locus_tag="VLL06_11325"
     CDS             complement(8888..>9291)
                     /locus_tag="VLL06_11325"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HSS31600.1"
                     /translation="AYGIMSHFDARLMPGSFWVNLQTKTETTNQAITGVLSEIKSIRD
                     TPVSDQELAEAKSYLIGSFPLRFDSTAKLAKVLAQVEFYGLGFEYFSEYQKWIDRVTK
                     EDVQRVAKQYLDPKRYALVVVGNIAKAKVKH"
BASE COUNT         1978 a         2351 c         2832 g         2130 t
ORIGIN      
        1 ggccagattg atggcggcga cccccatgac cagcaggcct gccagtacca tcctggacag
       61 gtagggcttc aggaacggaa gaaatcgtga gaaggtggac atgatgagga gcgatcttag
      121 aggaggtgat tatggaaaac caagtgagtg cggcagagga gagactcaag ccggaggtgt
      181 tgcggtagct acagctgtgc gatggactcg attaggttct tgttgatggc cacgaagtcc
      241 gaacgttcct tctcattgat caacacgtcg gtcaggctga tgaagagccg ttgctcttcg
      301 ttgatggcat cggacacacg attccgcatg ggaggaatga gaaagtcgcc aacgtagacg
      361 taggtcgcag tccggacgcg gatgcgaacc ttcttgcctt cgggcacgga cttctccttc
      421 ccttatacgg gtgagcctag cttttccgac gttcaaactt ttgacgccga cgcagctttt
      481 tgtacttgtg cttgcgcatt ttttttcggc gttttttcag cacactggac atgcgtgttc
      541 tctcctacga agcggcgagt gtagaagttt tcgccccaaa ttgcaaggtc aacaagcgac
      601 taaattgatc atgattggcc caaggccgag cgggcgggct ttggcggggt tgtgatgtga
      661 tgatgattgc atcacgggct gcttgacctg cctgatagcc atttctatac tgcagtcatg
      721 atattaccgt acaaacttcc actattcccg cttgtggttc tgctcagctc gatcttcttg
      781 atcagcgtcg gttgcggcgg caagaagatt caatatcccg acgatcacga acggtacctc
      841 cgtatcgatc gggcggtgga atcgctccgc caagcctatg tgaaaaaaga ttcatcggac
      901 ctcgcctcgc tcatgatacc gagtgaccag cttgatcgac tgagggaaga ggcccagggc
      961 gattttgaga catttagtgc gatcacgttg gattttgccg tcgagcgcat tatggtcgaa
     1021 ggggacaacg tcgatgtctt cgtacattgg caagggctgt ggaaaaaagg tgcagaggat
     1081 ccaggtctcc ggcagcgagg ccatacgcga ttgcaatggg ttggaaccca gtcgatactg
     1141 ttgcgcgggg ttcagggcga ttctcccttc ggcatgaaag cacgacaggc catggtcgac
     1201 cccacacgtc ctcctccgaa gaagtaatcc atgccatctc gtcgttccca tgctccggtg
     1261 caggccgatt tcctggtcat tggaagcggt gtggccggtc ttcgcgcggc gttggagtta
     1321 agccgtgcgg gccgggttat ggtactgacg aaagggcatc cgcttcagag cagttcgatc
     1381 catgcgcagg gcggggtggc ggtcgcgatg agtgaggaag acgatgtggc catccatcta
     1441 acggacacgc tcaaagcggg gcatgggctc tgtcgaaaac aagcggtgcg ggtcctcgtt
     1501 gaagaagggc ctgagcggat tcaagaatta atcggatggg gggccaagtt cgacaaagtc
     1561 ggcggcaagt ttgcggttgc tcgtgaggca gcccacagtc gaagccgcat tttgcgcgcg
     1621 cgcggggatg cgacggggaa tgagatggta cgagtcttga ttgctgaggt gaatcggcag
     1681 gaacgtattc agcgattgga tcaccacttt acggtggatc tcgtggtcga tgccggaaat
     1741 tgccgcgggg ccgttgtgtt ggatgaaggg tctggtcgcc agttcgtgct tccagctcgc
     1801 gcagtcgtgc tgaccaccgg tggggccggg cagatctatg cgaggacgac caatcccccg
     1861 aatgccactg gtgatgggat ggccatggcc ctgcgcgcag gcgcggtgct ccaagatatg
     1921 gagttcgttc aattccatcc cacggcactg tatctgccgt cgagtccacc gttcctgctg
     1981 tcggaagcca tgcgagggga aggtgcgcag ttgcgcaaca ataaaggcgc gctatttatg
     2041 cagcgttatc accccatggg ggcgttagcg ccgcgggaca tcgtgtcgcg tgcgatctta
     2101 gcggagatgg cggcgacgaa agcccgtcat gtctatcttg acgtcaccca cttaggtgcg
     2161 gaatttatca ggcagcgttt cccgacgatt tatgcgacgt gccttcgtta tgatatcgat
     2221 atcacggagg aatggatccc agtgtctccc agcgcgcatt atatgatggg cggggtctgg
     2281 actgatctga acggtgcgac aacggttccg gggctctttg ccgccggaga ggttgcctgt
     2341 agcggtgtgc atggagcaaa tcggctcgcg agtaattcct tactcgaagg attggtgttt
     2401 ggcatgcgcg cggcatcggc agccgtcgcg tttgccgacc aacagaaact tcccgcactg
     2461 tcgtctcagg agactacgct ccgaacgggc caattcggaa cactggagga tgcggaaaaa
     2521 ttgcgaagct cacttcggcg aacgatgtgg ggacaagtcg gcgtcattcg ttcgggcgag
     2581 tctctcattc gagcctgtgc gcaactgtca cggtgggctc aattggtgac ccagccattt
     2641 gcaagccgat ccgcactgga agtgaaaaat atggtgcagg tggcgcaatg tgtcgccgaa
     2701 gcggctctgt ggcgagagaa tagcgtgggg gcccattacc ggtcagattt tccagaggcc
     2761 gaactggctg gctggcgaca acacagtcgt ctatcgagcg gggaagttgt tagtgggaag
     2821 atatctcaga aatcgcgggg gttgctgttg tctccgcggg agcatatcgg ccgactggac
     2881 gctccgtgat gacgccatcg cggacaagga atgtgcggta gtatcgcaga actttccgga
     2941 catattgcct ggtttcatcg aacggtggaa gtgcctgata gcgatcgacc acattctctc
     3001 cggcattgta ggccgccagg gccagcggta agttcccatg gaaccggtcg agcagctggc
     3061 gtaagtattt ggtccctcca cccacattat catccggatc atacatatcc cgcacatcca
     3121 ggcgcacagc cgtctgcggc atcagttgca tcaggccgat cgcgccagcg cgagacaccg
     3181 ctcgcggatc aaagtctgat tcagctttga taacggctcg gatcaacgcg ggatgcagct
     3241 ggtgttggga agagtgccga cgaatgactg gttccaattc tcgttcggat aagatggagt
     3301 ggaaccgact ggattcgatc tcaacttttc tatagcgcga atctgatggg acgttggtca
     3361 aggagatcga gccgtcgtgc ccaataaact gatagatttc ggctgtggtt gccgggacgg
     3421 agaaggtcag cgagccactt gccgtgatca ctgtcgtggt gaggccgatg gccgcctgcc
     3481 gtctgaggga tgtcaatacg agggaaatgg tgttgtgcat gggtgcaacg ataccagtct
     3541 acttctgggt gtcaagaaac tgcagctctt gtgccaagag tccagttata aatagtgtgg
     3601 gaaaacaagg gggtttctga gggagtggcg ccgctagggt tctaaaaaac gcgctttgtt
     3661 ggcaataaaa tacgtatgga gctgccgggt aagaagtcct ggtgttccat tcccaaccgg
     3721 ttgtccgttc accatggtga ccggcatcac ttccagactt gtgttggtga taaaacattc
     3781 gtctgcctta tggattgcct cgatcccaaa atgcccttcc tcaaccggga tctgcttctc
     3841 ttgagcgaga ccgatgacaa tgtcacgggt gattccgtcc agcaagccgc aggcaagggc
     3901 aggggtacag agccgacctg cttggacgaa aaagagattg ctgacggtgc attctgtcag
     3961 atgtgattcc caattgagca gaatgctgtc gaaggcgtcg gctgcgatgg cttcccgctt
     4021 ggccaggata ttgttcaaaa aatttgtggc cttgatctga ggcgagagcg cgctgggcag
     4081 gttccgtctc gttcgggcga cgatcagctt cactccggca tgatattgtt cgggtggggg
     4141 agggtgaagc ggtttggtca tgatcaccac ggtgggggtc gggcagaggg ccggatcgag
     4201 accgatgtcg ccagttcctc gagagatcgt gatgcgaaga taggcgtcgg tgcgttcgtg
     4261 cccgacgtcg ttccgtgtca tcgcttcgtg gaggagcagc ggccacctgt gctcggagat
     4321 cgggatggtc agaccgatcg cgtctgcaga ccggtgaagc cgagcaagat gcagatcccg
     4381 catgaagatt cggttcccat acgagcgaat cgtttcatag actccatcac catagagaaa
     4441 gccatgatcg aagacggaga cgacggcctc ctcttctttc acgaaccgat cgttgagata
     4501 aatccacatg gttatgagtg ttgtagagcg ctgaagaacg cttgggcttt ctggagcgtt
     4561 tcctcgtatt ctttcgccgg gtcggaatcg gcgacaattc cggcaccgac ttgcagatac
     4621 cctttccctc cacaccatat caaggttcga atgacgatat tgagatcgag gtcgccgttc
     4681 cagccgaggt acccaaatga tccggtgtag gggccacggc gtaccggctc caactcctca
     4741 ataatttcca tgcaacggat cttgggtacg ccggtgatgg tccctccggg aaatagcgcc
     4801 tgaatcagat caaagggcgt ggcatcgggc ctcaaaccac cactgacatt ggacacgatg
     4861 tggctgacgt gggagtactg ttcaatcgcc attaattcat cgacctgcac gctgccgaat
     4921 cggcagacgc ggcccaggtc gttccgttcg agatcaacga gcatcaggtg ttccgcccgc
     4981 tccttttcat tcgcgagtaa ttcgccgacg aggcgttgat catcatgggt gtcgaccccg
     5041 cgaggcctgg ttccggcaat cgggcgcgta tcagcctgtc tgttgcggag acggatgagc
     5101 cgttcgggcg atgagctgat cagcgtgaca tcgtcgaagt ggatgaggcc tgagaaaggc
     5161 gaggggttca cggcttgcag acgtcgatag agttcctgct cgtggagctg tcgatccgtg
     5221 ccctcatcat atgaactcag aggtttcagg gtgaaacgat gtgacagatt ggcctggtaa
     5281 atgtctcctg cggcaatata ctcctggcag cgccggactc gttggaggta ggcatcccgt
     5341 atttgatctg gatgaaaggc catctgatcg agggagggaa gaggatccag gggggacggc
     5401 cttccgctca acctgacttc ccactcggcc agcctgtcca agccctcgcg atagagcttc
     5461 tctcgcggct ctcccacaaa ccgttccatg gggggacaaa agagaatctt tacaaggccg
     5521 gtctgatgat cgacggcggt gacgatgtca tagagaccga attgcaagtg agggagtagc
     5581 aggtcgtcct tggcgatgga cggaagtgtt tcaaattcgc gtacgaggtc atagctgagg
     5641 tagcccacgg ctccgccaca aaaatttggc aggcctggag gaaattcgat ttgcgggcca
     5701 gcgaatagcc ggcagaggct tccgaaggga tcgttcgaca ggtactcgcg tccgtcgtgg
     5761 gtccgaatcg aagcctgtcc ttgtcgcccc gtcagtaccg aaaatgggtt gcttcccaga
     5821 aatgaatagc gggatccgcc atcggtcccc tttccgctgt caagcaggaa cgatggccga
     5881 gaggaagatg caatgagacg gtagagttca aagggtgtcc tcttttggcc attcatcgtg
     5941 aggaccaagg gctgtggggg accgctcagg aacgatggtt gggagaaacg agtcatgggt
     6001 gaacagggat cgcgctacac atatgaatga ttcactatac gccactgtgc cggcagtgtc
     6061 acagtgcaga gtcggcttga caaattcagg atgattctgc ttgaattgcg cgcccgccat
     6121 cggcagagtc cttgcgttat gggctgcatt gcactctcct ccgtgggtac gacatgcccc
     6181 cctcacaaga tccattgtac acggggcttg gccaagcggt ccggatcgca accgatctgc
     6241 tcgctgcgct aatcgtcgga ggagcagtgg gatgggcatg tgacacctac ctctttgact
     6301 ccaccccctg ggcgatggtc ggaggtttgg tgttgggtat cgtggccggg atcaggaatg
     6361 cgtaccggtc ggcccagcga tggccgaaga cataacgccg tgcactagag aaagcacaag
     6421 agtgccatgg aagaaagtcc gcttcatcaa ttcgaacttc agaactggat cccgatctca
     6481 ctcggtggat tggacgtttc catcaataag gccgtggtct tcatgtgggt ggttgtctcc
     6541 ctggcggcgg tgctcatggt gatggcagga tcggcgcgca agctggtgcc cggcaagttg
     6601 cagagcctgg cggagatgat ggtggacttc attcgcagca tgatcatgga tacgatgggc
     6661 aaagatggga tgcgattttt ccctctcatc gccacgctct ttctctttat cctcttcagt
     6721 aatcttatcg ggttgatccc cggcacctac accgtgacga gtcagattat tgtgacggcg
     6781 gtgttttcct gcatggtgta cgggctcagc ctggttatag gattctggat acatgggata
     6841 aaatttctgg gcattctaat tccgcccggt acgcccgggt ggctgctgcc attgatgata
     6901 ccgatcgagt tgatcagtca gttggcacgg cccatttcac tggctgttcg gttatttgcg
     6961 aatatgacgg cgggccacgt gatgctcgcg gttctgttca gcctgacgat cggcggcgga
     7021 ttgttgatcg ggtggttgcc ctttgcattc acggtcgcga tatacggatt ggaatttggt
     7081 atcgcgttca ttcaagccta tattttcagc atattgacct gtgtctactt gggggacgca
     7141 tttcatttgc atggccatga tgagcacgcg cattagcgcg tgtgagttca gacaagggag
     7201 gagtaagaca taatggattc agcagcagca gggttgatcg ggatgggatg tgccgcggca
     7261 gggtttgccg gagcaggtgt gggtatcggg tacatcttcg ggaagatgat tgaagtggtg
     7321 gcacgccagc cggaagcaga agctcgcgtc acgaagtata tgtggatcgg gtttgcgctg
     7381 gtggaagcca tcgcgctgta tggcttggtc attgccttca ttatcatggg ctttcgcaag
     7441 taggagcagg cttaggtctg ggtaaaggtc gggggcgcat cccctctcag cctcaccgcg
     7501 gcctttctga tgtgaactat gccacagttt gaacctgatt ttttttcttc cttgatattc
     7561 tgggaagtca tatcgtttgc cattctcttg ttcgtccttt acaagtacgc gttccccggc
     7621 atcttgggtg ccttggaaga gcgggaaaag agaattaagg acagcctcga tcaagccgag
     7681 catcaccgtt cagaggcaga acggaaactc aaggagtatg aggccaagct gaactccgcg
     7741 gcgaaggatg cggaaggaat cctcgccgca gccaaggaac gagtgcagcg gctcatggaa
     7801 gaaaatgagc aacggatgac gaccgaagcc gagcggatca agggtgacgc gacgcgtgaa
     7861 atcgatcatg agcgtcgcaa ggcgattcag gatattcgct cccacacgac cgatttggcc
     7921 ctgatggttg cagagaaagt ggtgcagcga agtctaaatg aggcggacca ccggaaattc
     7981 gcagacgagg cgctcgaggc gttgtcaaaa tcctaccaac gataatacgt ggcctgggta
     8041 gggtcagcct aggctgaccc cgcccggtct gtatccttcc aaatccacac agacgaagcg
     8101 aaacccggct ttgcgaagat gggcactgat gcgcgcgcgc acgtcaggct gattcaagag
     8161 agaaaattct tctgcgccga cctcgattcg cgccacttct ccatgctccc gtacgcgcac
     8221 atgccgaaat ccttccgctt gtagaatctc ttcagcttgc tccacgcgtc gcagactgtc
     8281 gatcgtgatc ggggtgcctc ggggaatcct ggaagaaaga catgcggcag ccggcttatc
     8341 ccaattcgag agtcccaagg tctgtgcgag tgcccgcaca tcggattttg agagagaggc
     8401 ttcgaccaac ggacttcgga ctccccactc gcgtgccgct ttgattcctg gtcgatcatc
     8461 cccgagatcg tcgagattcg tcccatccac aatccagcgg cttgcgcgcg gttcgcgcag
     8521 cttgtccagt aattgataga ggtctgtctt gcagtggaag cagcgggttg catcgttctg
     8581 tacgaaggcc gggacatcaa gttgatcggt gtggaccagt tcatgccgcg ccccgatctc
     8641 taaggcgacc tgcttggcac catcgagttc aatcgtggga aacgtcggtg agacagcagt
     8701 caccgccagc gcatgttcac cgagttgttc atgggcgatt ttcaggacga tggtgctatc
     8761 gatcccgccg gagaaggcga ccaggacgga ctgcatgtcc gagatcacct ggcggagtgt
     8821 cgtgagtttt ccttggaggg attcggcggt catgaactct tgtccgtaga tattgtgcca
     8881 cgactgccta atgcttgacc tttgctttgg caatgttgcc caccaccact aaggcgtatc
     8941 gtttggggtc cagatattgt ttcgccacgc gctgcacgtc ttccttcgtg acccggtcta
     9001 tccatttttg gtattccgaa aaatattcga atcccaggcc atagaattcc acttgtgcaa
     9061 gcactttcgc aagtttcgcg gtggaatcaa atcggagcgg gaagctgccg atcaaatagg
     9121 acttggcctc agctaattcc tggtcggaca cgggtgtgtc acgaatggat ttgatttccg
     9181 ataggacgcc ggtgatggct tgattcgtgg tctccgtctt cgtttggagg ttgacccaga
     9241 atgaaccggg catcagacgg gcatcgaagt ggctcatgat cccataggcc a
//
LOCUS       DATIPF010000457         3576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_706111, whole genome shotgun sequence.
ACCESSION   DATIPF010000457 DATIPF010000000
VERSION     DATIPF010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706111"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            515..862
                     /locus_tag="VLL06_11330"
     CDS             515..862
                     /locus_tag="VLL06_11330"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS31601.1"
                     /translation="MATQVNPVECRSSERIALQCSIIVATGVQTGEGRVLNVSKSGCL
                     VEAPILIKAGDYLQMRLFLPNTDLSMCVSVAIVQWALGFQFGVEFIEVDEKHRTRLNH
                     FIATGKDPWTLAL"
     gene            882..1145
                     /locus_tag="VLL06_11335"
     CDS             882..1145
                     /locus_tag="VLL06_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31602.1"
                     /translation="MVAAHRRGQADPSRTAKEGNTWQALQREVLYFLTERGPISGEVF
                     RTGFDEQRRREGRSYERRRNTSSLSVIRLSEYHTLGSEYLTYS"
     gene            2058..>3576
                     /locus_tag="VLL06_11340"
     CDS             2058..>3576
                     /locus_tag="VLL06_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31603.1"
                     /translation="MMNTCWLLQACIVMAVGAGLVSCTKEQKAHGLVQPAQNIQPVSP
                     CHPAGVSSSGTPEFCVNVTEIQLLNHETQADVRLSLINRTGHRLYVALIGLTALTDSS
                     GRKWNTGDSRGLGSPNTPVPVEPDGEMQGAISFYQNGQVPADLSFSLRGEIGIMTMDT
                     RGQAVPGQMAIKRGIYLSGIHTQHQPQQSAGVMEQHKDTQPGHESPRRANLTAPKPSA
                     SSKSSASGAVVDAAGSGKPHSQKFASANVSHSPDQRSVTIDSPAANVATSQSRSRSVG
                     SSGSGPEVMGLRIGMTPDQARQKFKSHGFGSSTKSPNRPFDSYTESSNMLTFSLPGQA
                     LQPVPNTKYIAQMSGAISDIKIPATENSGDVLTIFFGPIPGEGGIVLLNRTEYLPASK
                     RPTVAAFAKTLVETYGTPTELQPGSPGIYRWRYDSSGTLYNPTPATSFVGCPRLKPGI
                     VEFQPSRSPLMIQEYKQSVPKCGAIFLEVDVGFADFKFAGPGTLIKNYTTHMTGLD"
BASE COUNT          776 a          971 c         1013 g          816 t
ORIGIN      
        1 ggggtcaacc ggtcgatgga cccccggccg ggctgacgcg gcctagccgg aagtccttgc
       61 gccgtcgttc gcaaccccga aggcgaatca tcgtcctcga gaatggtcgc tcagccctca
      121 gctgggcggt gagagcctcc accatcagca acgcattccc ctcagcccga gtgttgacca
      181 gtacatacgc acgctgagat gggaaactgc ggtccttgca gaatggctcc gttccccccc
      241 ttcgaattag ttccttcgag tctttcgcca ttcaccccct gccgataccc ttcttataga
      301 gcggacgttc acaggctgaa tggcaaggag gtgtcatcat ggctactctg cgcgcgcttg
      361 tcggagcagg ctctgtgctg gccttcatgg gattcgtggt gttcgttccg gttggttggg
      421 ccgtggtcct cgtcgccgcc tcggcgaccc catagaattt tgcagaaccc agttgcggct
      481 actcgcagtg cggttataga tgggaggtta tacgatggct acccaggtga accccgtaga
      541 atgtcggtcg agtgagcgga ttgcgcttca gtgttcgatc atcgtggcaa ccggcgtaca
      601 gaccggcgag ggacgagtcc tcaacgtatc caagagtggc tgtttagtgg aagcccccat
      661 actcatcaaa gcaggagatt atctacagat gcgcctcttt ctgcctaata ctgatctgtc
      721 catgtgtgtg tcggtggcga tcgtccagtg ggccctgggt ttccagtttg gggtcgagtt
      781 tattgaagtg gatgagaagc atcgcacacg cctaaatcac tttatcgcta cagggaagga
      841 tccatggaca ttggcactat agctggcgca gatggaggcg gatggtagcc gctcaccggc
      901 gtggccaagc cgacccctct cgaaccgcca aggaggggaa tacgtggcag gcccttcagc
      961 gagaggtcct ctattttctg acggagcgag gcccgataag tggggaagtc ttccgcaccg
     1021 gttttgacga acaacggcga cgagagggcc ggtcctacga gagaagaaga aatacaagct
     1081 cattgagtgt gattcggctc agtgaatatc acacgcttgg ttcggaatac ctcacgtaca
     1141 gctaaacgtc tttcacacgc gccacggcac tctgtgtcag agagtgacct cacagcgaag
     1201 cagacgtgaa gagcctgcaa gggataaact cgcgaagttg gtcagtcagc aggtgtcaat
     1261 gtcgcaggtg gccggctgac gtttctccgt ttctcgatat cctgatcgat tatctttaag
     1321 gcgtccaatc gggactcgag ctccgcaata tatttatcct tctctctgat ttgtttttgg
     1381 agggactgca cgagacgtga ttgagggaca cccagttcgg atgtacgttc gatatgggcg
     1441 gcaggtcgtg tgtcgcagct gatgaaggtg aagaccccaa gccaagcgac tccaaggaag
     1501 ctccaggccc tctgacggat catggccatg gtctagatgt gccatgagat acttcggctc
     1561 gcaagaatat tgaggaattt gactgaccac caccgtcatg atgcctcgcc gagtcggctg
     1621 attgtcagtc acgactggtg ccatctttct tgggccgcgc gtttggctat caggccccgg
     1681 tccttttctg tcagctccgg gatatgcgcg gtctccttgc ggttatctcc attctgtcgt
     1741 gtgccgggcc cgccgtggca gtgcgttcac cggggccatt tcttaggttg taggcctaca
     1801 tttctggttt tggctcaata gcttgacggc ttgcacaatg gtacgtagca tcctcctcaa
     1861 gacgtgcgaa ggcatgcgga cgttcctgtg ccctcgcctt cctgtttcct tcctgcgcgg
     1921 ctgactccct cgccggcccg gttttttgac gtacgacgtt tctttgtgtt gaacgacagg
     1981 agcgaaacca ttcacctgcc ttagaggaga ggcgtcacat ggatactccg ttcaacgttc
     2041 ttctgcaaag aagctcgatg atgaacacct gttggctgct gcaagcctgc atcgtcatgg
     2101 cagtcggagc ggggcttgtg tcgtgcacaa aagagcaaaa agcccatggt ttggtccagc
     2161 cagcgcagaa cattcaaccg gtgagcccct gccatccggc aggagtgtcc tccagcggca
     2221 cgccagagtt ttgcgtgaat gtgacggaga tacagctgct caaccatgag acacaggctg
     2281 atgttcgttt gtcgctcata aaccggacag gacacaggct ctacgtcgct ttgatcgggc
     2341 tcactgctct gacggacagc agcggaagaa aatggaatac gggggatagc agaggcctcg
     2401 gtagccccaa tactcccgtg ccggtagagc cagatggaga aatgcaggga gccatctcgt
     2461 tttaccaaaa tggacaggtc cctgcagacc tgtccttctc gctgcgtgga gaaattggga
     2521 ttatgacaat ggacactcgc ggtcaagcgg tcccaggtca gatggcaata aaacgcggga
     2581 tttacctctc gggaattcat actcagcacc aaccacagca gtctgctggt gtgatggaac
     2641 agcataagga cacccagcct ggccacgagt ctcctcgtcg cgccaatctt acggcgccca
     2701 agccatcggc ttcgtccaaa tcatccgcgt ccggggcagt cgtggatgcg gcaggcagcg
     2761 ggaaaccgca tagccaaaag ttcgcatcgg ctaacgtcag tcattcccca gaccaaagat
     2821 cggtgacaat cgattctccg gctgccaatg tcgcgacaag ccaaagcaga tcgaggtcgg
     2881 tcgggagtag cggatcgggt ccggaggtga tgggtttgcg catcggcatg acgcctgatc
     2941 aggcccgcca gaaattcaaa tcgcatggtt tcggatcatc gacaaaatct ccgaataggc
     3001 catttgacag ttatactgag agctcgaaca tgctgacatt tagtcttccc ggacaggctc
     3061 tgcagcctgt gccaaacacg aaatatatag ctcagatgtc cggtgcgatt agtgacataa
     3121 aaattcctgc gactgaaaac tcgggtgatg tcttgaccat attctttgga ccgattcctg
     3181 gagagggggg aatcgtgttg ctcaatcgga ccgagtatct tccggccagc aagaggccga
     3241 ctgttgccgc ttttgcgaag acactcgtag agacatacgg gactcccacc gagttgcaac
     3301 cgggttcccc cggtatctat cgctggcgct acgatagtag tggaacactg tataacccca
     3361 cccccgccac gagtttcgtc ggttgtccac gactgaaacc gggcatcgtc gaattccagc
     3421 cttcaaggtc ccccttgatg attcaggagt ataaacagtc ggtgccgaag tgtggcgcca
     3481 tcttccttga ggtggacgtg ggattcgcag attttaaatt tgctgggcct gggacgttga
     3541 ttaaaaatta caccactcat atgacaggac ttgatg
//
LOCUS       DATIPF010000458         7505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_882410, whole genome shotgun sequence.
ACCESSION   DATIPF010000458 DATIPF010000000
VERSION     DATIPF010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7505
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882410"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..2083)
                     /gene="ftsH"
                     /locus_tag="VLL06_11345"
     CDS             complement(275..2083)
                     /gene="ftsH"
                     /locus_tag="VLL06_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HSS31604.1"
                     /translation="MPVNKKTSFSILYVLLAVFAVVLVHDFIVAMQKVEELPYSEFKT
                     LMAAGKVSEITVTQQRLTGKLKSEQGSKEPKLFTTVRVEDPDLVKELNAHHVTFTGVI
                     ESSFLRDLLSWFVPALVFAGIWFFVIRRFGQGQGGFMTVGKSKAKIYAEKDTKTTFAD
                     VAGVDEAKDELREVVEFLKTPEKFTRLGGKIPKGILLVGPPGTGKTLLARAVAGEAGV
                     TFFSISGSEFVEMFVGVGAARVRDLFDQAKVKAPCIIFIDELDALGKARGMGPMAHEE
                     REQTLNQLLVEMDGFDPRIGVILMAATNRPEILDSALLRAGRFDRQVLVDRPDKIGRL
                     AILRLHAKAVALGPDADLEVIAGMTPGFSGADLANIINEATLLTVRRNKEEVGLSELQ
                     EAVERVVAGLEKKNRVLNQVEKERVAYHEVGHALVALSFPGSDPIQKISIIPRGIAAL
                     GYTLQLPTEDRFLMAKTELENKIAVLFGGRIAEELMFGEASTGAQNDLVKATDIAKSM
                     VKSYGMSDKLGTVALDRERQPLMMQIQAPQEKGDYSEETAREIDCEVRRIIDEQYERV
                     KRLLAEKKPALIAGATVLLEREAISGSDLKAIMDKQ"
     gene            2632..3312
                     /locus_tag="VLL06_11350"
     CDS             2632..3312
                     /locus_tag="VLL06_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSS31605.1"
                     /translation="MNETAVRLMQGPLSLEGILGLPAEALGMVVFAHGSGSGRFSPRN
                     NFVARHLQQGTVATLLLDLLTPDEAEDRRKVFDIDLLADRMLWAKAWLEQETSTKDLG
                     IGYFGASTGAGAALQAAARDPSSIRAIVSRGGRPDLAGSYLPSVTAPTLLIIGGDDEP
                     VIEMNRAAYRLLSCPKRLVIVPGASHLFEEPGTLEQVAEHARLWFQRHLHPASPESTG
                     PRTKEPYR"
     gene            3309..4172
                     /locus_tag="VLL06_11355"
     CDS             3309..4172
                     /locus_tag="VLL06_11355"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSS31606.1"
                     /translation="MKTLLAVDGSNNSYEAVHALNFFARAEELILLHALDVPRPTYPM
                     MVPEVQEELYKALEQSMREDGERLLDRIQSLLPIHSGPSTKQLRIGSPAELIVLTAEE
                     QKTDLIVMGARGLRPVKERLFGSVSHRILTLAHCATLIVNGSVKAMKQILLPLQGLSD
                     AESAVRFLQLKPFHDPVEVTLLTLLPSTQPPWPVNAASAETLEEQALLSARDYIDGVA
                     ERLRAIGYQARGIALFGTPAAMILQEATTRHSDLILMGTRGRQGITRFVLGSVSHAVL
                     HRMPCPVLAFH"
     gene            complement(4332..4808)
                     /locus_tag="VLL06_11360"
     CDS             complement(4332..4808)
                     /locus_tag="VLL06_11360"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HSS31607.1"
                     /translation="MMVLLVWFHLLAAIAWIGGMLFLSVVLVPVLRCEPFASQKAVLF
                     RAIAGRFRAVVWGAIAVLLFTGPLLLHQRGIPITDPSAWPTVLVAMLGLVALLLLLTL
                     LHDFILGPRVLQIAQIHTDSRTRFEHSLVLWSPWVARFSFILALAVLFTAVVLVRT"
     gene            complement(4914..5252)
                     /locus_tag="VLL06_11365"
     CDS             complement(4914..5252)
                     /locus_tag="VLL06_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HSS31608.1"
                     /translation="MKMIEAIVKPFKLDDIKDALVEIGVQGMTVSEVKGFGRQKGSHE
                     TYRGAEYTTDFVPKVKIELYVADNMAKRVIETIARAAKTGSVGDGKIFVSDLESVLRI
                     RTGETGESAL"
     gene            complement(5546..5950)
                     /locus_tag="VLL06_11370"
     CDS             complement(5546..5950)
                     /locus_tag="VLL06_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS31609.1"
                     /translation="MVQGRNRAVVLIAEDDHEMRSLLCDELNDLGVFIREATDGDEAL
                     QSVLDARPSLILTDLRMPAGGLDYIARLRTFAPGVPMVLITSFGDPQMKADALALGVE
                     AYFDKPVRLHELKGAVRRLLGPVLEGKPAEAL"
     gene            complement(6220..7311)
                     /locus_tag="VLL06_11375"
     CDS             complement(6220..7311)
                     /locus_tag="VLL06_11375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS31610.1"
                     /translation="MSLDSFDREQQWRLIVEAAPNGMLLVDEGGTIVMVNTSVLRQFG
                     YGRDELLGKSVEMLIPEPLRSDHRQYRAGFTRTPQVRSMGSGRELFGIRKDGSELAVE
                     IGLTPIQTAKGMRVLASIVDISERKRLEMQLRRAERLAELGTLASGMAHEIGTPMNVI
                     LGRAEYLLQRATDEGMKKGLTTIITQVERITKVMKQLLAFARRRSPEWRTVDLGEIVD
                     DALEMFQERIAERCITIDKAIESSLPPVSADRDQLIQVLINLVMNSIHAMPEGGQLKL
                     SLAREVSSVCLRLADTGLGMPEEIRSKVFEPFFTTKDFGMGTGLGLTVVKGIIEEHGG
                     TIDVESIVDKGTTFWIRLPLDKAQTASTS"
     gene            complement(7308..>7505)
                     /locus_tag="VLL06_11380"
     CDS             complement(7308..>7505)
                     /locus_tag="VLL06_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSS31611.1"
                     /translation="GARGDRRVLDDAAERTLPLDQIEKEYILKILEKTGGNKYQAAHM
                     LGIDRKTLYRKLGVIEGQTPG"
BASE COUNT         1678 a         2288 c         1950 g         1589 t
ORIGIN      
        1 cataagggtc atggttcctt cagggaaacc gattcgatcg accttgcagg acaacacacg
       61 ggtgggagga gactcgacgt ggaagagcga acacttgagg ctcgatgagc ccccattgat
      121 cgtcagaatg aaaccgtgag agtcatgtcc gggatcgcgc aggctcacag accacctcaa
      181 gtcaataaca atacctactt cacaccgatc ccagactctt ccgcataata gatgtgtgca
      241 gcgttcgcgg tccttgccga tagaagtctg cgcctcactg cttgtccatg atggccttaa
      301 ggtcgctgcc gctaatcgct tctcgctcga gcagcacggt ggcccctgcg atgagtgcgg
      361 gtttcttttc ggctaagagc cgtttcacac gctcgtattg ctcatcgatg atacggcgca
      421 cttcgcagtc gatctcacgg gcagtctctt ctgaatagtc gcctttctcc tgaggcgcct
      481 ggatttgcat catgagaggc tgacgttcac gatcgagcgc gacggtcccc agcttgtcgc
      541 tcatcccata ggatttaacc atgctcttgg caatgtccgt ggccttgacc agatcgttct
      601 gtgctcccgt ggaagcctcg ccgaacatca attcttccgc aatccgtcct ccgaataaga
      661 cggcaatctt gttctccagc tccgtcttgg ccatcagaaa tcgatcctcg gtcgggagct
      721 ggagggtata gcccaatgcc gcaatcccgc gaggaatgat ggaaatcttc tggatcgggt
      781 ccgaccctgg aaacgacaat gccaccaatg catggcccac ttcatggtag gcgacacgct
      841 ctttctcgac ctggttgagc acccgattct tcttttccaa tccggcaacg acccgttcca
      901 ccgcttcttg caattccgaa agaccgacct cctccttatt ccgacgcacg gtcaagagcg
      961 tcgcttcgtt gataatgttg gcgagatcgg cccccgagaa gccgggggtc atcccggcga
     1021 tgacctcgag gtccgcatcg ggacccagcg ccaccgcctt cgcatggagg cgcaatatgg
     1081 ccaaacggcc gatcttgtct ggacgatcaa ccaagacttg cctatcgaac cggccggcac
     1141 gcaagagtgc ggaatccagg atttcaggcc gattggtcgc agccatcaga atgacgccga
     1201 tgcggggatc gaaaccatcc atctcgacca gcagctgatt caaggtctgc tcacgctctt
     1261 catgcgccat gggtcccatc ccacgggctt tcccgagtgc atccagttcg tcgatgaaga
     1321 taatgcaggg cgctttgact ttcgcctgat cgaacaggtc ccgcacacga gcggcgccga
     1381 ctccgacgaa catttccacg aactcagacc cgctgatact gaaaaatgtc accccggctt
     1441 ctccggccac agcacgggcc aggagagtct tgccggttcc cggagggccg acgagtaaaa
     1501 tccctttggg aatctttcct ccgagacgtg tgaacttttc aggcgtcttc agaaactcga
     1561 ccacttcgcg cagttcatcc ttcgcctcgt ccacgccggc cacatccgcg aaggtggtct
     1621 tcgtgtcctt ctcggcatag atcttcgctt tcgactttcc caccgtcatg aaccctcctt
     1681 gaccctgccc gaatcgccga atgacgaaga accaaatgcc ggcaaagacc agcgcaggaa
     1741 caaaccagga gaggagatcg cgcagaaagc tactctcaat cacaccggta aacgtgacat
     1801 ggtgtgcgtt gagctccttg accagatcgg ggtcttcaac ccgaacggtc gtaaacaact
     1861 tcggctcttt ggatccctgt tccgatttca acttcccggt caatctctgt tgagtgaccg
     1921 taatttcact caccttgccg gccgccatga gcgtcttgaa ttcgctgtat ggaagctctt
     1981 cgaccttctg catggccacg atgaaatcgt gtacgagaac gaccgcaaaa accgcgagga
     2041 ggacatacag gatcgagaaa gatgtttttt tattcactgg catcgcgata cgctcctctc
     2101 actgtcacga acctcacgag atgttgaagt cctccgtcag tcgaggtcac acggtgggaa
     2161 ccggctccga tcagaatacg gacgccctca gtagaaaaca cgacgactcg cattgtacac
     2221 gttccagcct gtgcagggat ctacacaaac catccagtcg catgtgctca ttgggagctg
     2281 caaagaaaag accctcacac cgaacgagca tgctcggtga ttcttgttgg aatattgaga
     2341 gcttccaacg gctatggatg gatgaaagga agataggagg gagtcctgct acagtctggc
     2401 ttttcactct gtggcaaaag gcgacagaaa acgtgaaccg tgagacgcga aaagaaggac
     2461 gagaccgaat gactttcact tttcacacct tatgagtcag ccctcaccct caccatctca
     2521 cgctgctgtt caggcaaggg catcagattt gctgcacctc tctgtggaac aaaagtggag
     2581 acgggatgag aggcgaagtg ccccgattcc cggccgacct gagggacagt gatgaatgaa
     2641 acggcggtca gacttatgca aggccctctc tcccttgaag gaatcctcgg ccttccggcg
     2701 gaggcccttg gaatggtggt cttcgcccat ggaagcggca gcgggcgatt cagcccgcgc
     2761 aacaattttg tcgcccgcca tctccagcaa gggacagtgg ctaccctgtt gctcgatctc
     2821 ctgactccgg atgaagccga agaccgccgc aaggtcttcg atatcgacct cctggcagat
     2881 cgaatgttgt gggctaaagc ctggctcgaa caggagacca gcacgaagga cctgggcatc
     2941 gggtatttcg gcgccagcac cggagccggt gcagccctgc aggcggctgc cagggatcct
     3001 tcatcgattc gggctatcgt gtcacggggc ggcagaccgg acctggccgg atcctatctc
     3061 ccgtcggtga cggcaccgac gctgctgatc atcggcggag atgacgagcc cgttatcgaa
     3121 atgaatcgag cagcctaccg acttctttca tgcccgaaac gtctggtgat cgtgccgggc
     3181 gcctcgcacc tcttcgaaga gcccggcacg ctcgaacagg tggcagaaca tgcgcgtctc
     3241 tggtttcagc ggcatcttca tcctgcctct ccggaaagca caggtccgcg aacaaaggag
     3301 ccttatcgat gaaaacacta ctcgcggttg acggatcgaa taattcctat gaggccgttc
     3361 atgcgctaaa ctttttcgcc cgggcagagg aactcatcct ccttcacgca ttggatgtcc
     3421 cgagacctac ctatccgatg atggtgccgg aagtgcagga ggagctctat aaggctctcg
     3481 agcagagcat gcgggaagac ggcgagcggc tgctcgatcg gattcagtcc cttctcccga
     3541 tacactcagg cccctcgacg aaacaactac gaattgggtc tccggcagag ttgattgtat
     3601 tgacggcgga ggaacagaaa actgatctca tcgtgatggg cgcacggggt cttcggccag
     3661 tcaaggagcg cctgttcggc agtgtgtcac accgcattct caccctggct cactgcgcca
     3721 cattaatcgt gaatggatcg gtcaaggcca tgaagcagat cttgctgccg ctccaaggtc
     3781 tctccgacgc cgaatccgcc gttcgttttc tacagctgaa accgttccac gatccagttg
     3841 aggtgacact gctgacccta ttgccctcga cccagccgcc atggccggtc aatgccgcct
     3901 ccgcagaaac actggaggag caagccttac tgagtgcgcg cgattatata gacggtgtgg
     3961 cagaacgcct gcgcgccatc ggctatcagg cacgcgggat cgccttgttc ggaacaccgg
     4021 ctgccatgat cctccaagaa gctaccacac ggcattcgga cctgattctg atgggcacaa
     4081 ggggccgaca gggaattacc cgcttcgtgc tcggcagcgt atctcatgcc gtgttacacc
     4141 gaatgccctg cccggtactg gctttccact gagaagacaa ccggcggata gacgcagaaa
     4201 ccacgcgaga agagttcgag gtccaatttc aagatgttct gaacttcgaa cctcgaaatt
     4261 ctgattgcac ctgcgcactg ggtgcgcccg tctcgcaatg gagagcgccg acgaacctct
     4321 ttaggagcct gctaagtccg cacaagcacg acggcggtaa acagaacggc cagcgccaga
     4381 atgaacgaga aacgagcgac ccagggagac cacaaaacca gagagtgctc gaaccgagtc
     4441 cggctgtctg tgtggatctg tgcaatctgt aagacccgag gtccgaggat gaaatcgtgg
     4501 agcagggtca acagaaggag gagcgccacg agccccagca tggccaccag aactgtcggc
     4561 catgccgatg gatcagtgat cggaattccc cgctgatgta acaacagggg acctgtgaac
     4621 aaaaggacgg cgatcgcacc ccagaccacc gcgcggaatc gcccggcaat ggcccgaaag
     4681 agtactgcct tctgtgacgc gaatggctcg cacctgagca ccgggaccag tacaacagac
     4741 aggaacagca tgccaccgat ccaggcaatg gcggcgagaa gatggaacca caccagcagg
     4801 accatcatcg acgctgccaa tccactgatc aacctttcat cgcaagctgc ggaactatca
     4861 ttccgcgcag ccgggctaac acctcgccgg cgacccttgc tccgacagca tcgtcacagc
     4921 gcgctttctc ccgtctcacc ggttctgatc cgcaacacgg attcgagatc cgacacgaag
     4981 atcttgccgt ctccgacgct gccggtcttc gcggcgcgcg caatggtttc gatcacgcgc
     5041 ttcgccatgt tgtctgcgac atacagttcg atcttcacct tgggcacaaa gtcggtcgta
     5101 tactcggccc cccggtaggt ttcgtgactc cccttttggc gtccgaatcc tttgacttcc
     5161 gacaccgtca tcccctgcac tcctatttcc actaacgcat ccttgatgtc atccaatttg
     5221 aacggcttca cgatggcttc aatcatcttc atgtcgatcc ctccctatcc aggctagtac
     5281 ctggaattgc ctgcatgatt gtcggtcgat tgtatcacgg tccgattatg aataggcacg
     5341 cccgttaggc tagttgagac accgtctcag gcccttaccc gctcagatca ttccgcatcc
     5401 ccaatcatca accgtgagga aatggtcgag cagagagtgg atggagtacc catataggcc
     5461 agtacagcaa acggatggac tgcgaggtga ttcctctcga gatccggcca tctcatacct
     5521 ctcacatgct atgacggtcc agagcttaca aggcctcagc cggttttcct tctaataccg
     5581 ggcctaagag acgccgtacg gctcctttga gttcgtggag gcgaaccggc ttatcgaagt
     5641 aggcctcgac gccgagagcc aacgcatcgg ctttcatctg aggatctcca aatgaggtga
     5701 tcaacaccat cggcacgccc ggggcaaacg tccgaagcct ggcgatataa tccaaccctc
     5761 cggccggcat tcgcaggtcc gtgaggatca acgaggggcg agcgtcaagt accgactgca
     5821 gtgcctcatc cccatcagtc gcttctcgga tgaatactcc caaatcgttc agctcatcgc
     5881 ataggagact tcgcatctca tgatcgtctt cggcaataag caccaccgct cgattcctcc
     5941 cctgtaccat cccgccctcc ctgcatcaga ctcccttcac gttctgatgg tactccatgc
     6001 acaacccatg caagacggtc tctccctctg ccggaacgcg atggattcca gaaccgagct
     6061 aacttgctta agattttctc gacatgcgca gggcagggga agagaacaca ccagggcctg
     6121 atgaatatgg caacagatgc caccttcgct tctgttgcag attgcgacag atgaatgacc
     6181 gctgtggatt gaatctctga gagagaggat tgaggtgagt taggaggtcg acgcggtctg
     6241 agctttatca agagggagtc ggatccaaaa ggtcgtaccc ttgtcgacga tactctccac
     6301 atcgatggtc ccgccatgtt cttcgatgat acctttcacg accgtgaggc ctagaccggt
     6361 ccccatccca aaatctttcg tcgtaaaaaa cggctcgaat acctttgaac ggatctcttc
     6421 cggcatcccc aggccagtat cggccagtct gaggcagacg gaactgactt cacgagcgag
     6481 gctcagcttg agctgccctc cctccggcat ggcatggatg ctattcatca caagattgat
     6541 gagcacctgg atgagttggt cccgatcggc actgacagga ggcaatgacg attcaatcgc
     6601 cttgtcaatt gtgatgcaac gttcggcaat ccgttcttga aacatctcca gagcatcgtc
     6661 cacgatctcc cccaggtcga cggtccgcca ctccggtgac ctccgccggg caaaggcaag
     6721 gagttgtttc atcaccttgg tgattcgctc aacctgcgtg atgatcgtgg tcaggccctt
     6781 cttcatcccc tcatcggtgg cacgctgaag gagatattcc gcacgtccga gaatcacgtt
     6841 catcggcgta ccgatttcat gcgccatgcc cgacgccagt gtgcccaact cggccaatcg
     6901 ctcagcccgt cggagttgca tctccagacg tttgcgttca gaaatatcca cgatcgatgc
     6961 caacaccctc atccccttgg ccgtctgaat aggcgtgagt ccgatttcaa cagccagctc
     7021 actcccgtct tttcgtatgc caaatagctc gcgcccgctc cccatcgatc gtacctgagg
     7081 agtccgtgtg aacccagctc ggtactgccg gtgatctgat cgtaacggtt ccggaatcag
     7141 catctcgacc gacttgccaa gcaactcgtc cctcccatag ccgaactgac ggagtaccga
     7201 ggtattcacc atcacaatcg ttccaccctc gtcaacgagc agcataccat tgggagcggc
     7261 ctccacaatg agacgccact gttgctccct atcgaacgaa tccaggctca tcctggcgtc
     7321 tgcccttcga tcacgcccag cttccggtag agcgtcttgc gatcgattcc gagcatgtgg
     7381 gccgcctgat acttattgcc gccggtcttc tcgaggatct tgaggatata ttctttctcg
     7441 atttgatcca gcggcagggt ccgttcagcc gcatcgtcga gcacgcgccg atcgccacga
     7501 gcccc
//
LOCUS       DATIPF010000459         3951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_706540, whole genome shotgun sequence.
ACCESSION   DATIPF010000459 DATIPF010000000
VERSION     DATIPF010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706540"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VLL06_11385"
     CDS             <1..915
                     /locus_tag="VLL06_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSS31612.1"
                     /translation="REGTVTELAKNLSTGQLDDLSRLVKVKQSPVDDPTGPAVETEKP
                     GIILGKELAMRLGVFVGDTVNVVSPPAGPISAIGMVPKIRTFALVALFQSGMYEYDSS
                     LAYIDLAEAQKFFNMGQTVTGIEIKVEDVFHAAETARSVEQSLGFAYGARDWMQMNRN
                     LFSALKLEKTMMFLLLVLITIVASFNIVSTLTMIVTEKQKEIAILKAMGATRKSIRRI
                     FMLNGLIIGLSGTAIGIPLGYAFLWLIQTFWTFDPTVYYISRIPVHVQALDVMLVAGS
                     AIVISFAATVFPSLQAAKLEPVAALRYE"
     gene            962..1624
                     /locus_tag="VLL06_11390"
     CDS             962..1624
                     /locus_tag="VLL06_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSS31613.1"
                     /translation="MITVTNLYKSFPMGGQTLTVLKGVNLQIQRGELIAIVGASGAGK
                     STLLHIIGTLDRPTTGTVHFDSQDLFQLAEGEQADFRNRRIGFVFQFHHLLPEFTALE
                     NACMPALIQRHNLADIEPEATALLQEVGLGDRLHHKPGELSGGEQQRVAVARALLQKP
                     DLVLADEPTGNLDTHTGEALFAMMRELNKARGTTFVIVTHNDKLSAQADRIVHMQDGQ
                     IV"
     gene            1781..2926
                     /gene="traF"
                     /locus_tag="VLL06_11395"
     CDS             1781..2926
                     /gene="traF"
                     /locus_tag="VLL06_11395"
                     /inference="COORDINATES: protein motif:HMM:NF025106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HSS31614.1"
                     /translation="MSCYRTLCAAAIIIGLMIPAQALALEFVTVGPRAMGMGEAGVAV
                     TTDALATYWNPAGLAMTQTVDVRIQGSGQVIDRLGIANAVHDLEEFNTSDSSAANLAK
                     AQNIANRINRPGATVSVNGSAGLYVKGHLGEHAFGLNVSDVATGGGFVSTPIQATQPG
                     GAGTPITLAGQMALLGLEARQVAFSYAYAFSDKTFSFGVTGKILQGAAYSGSTTLTGG
                     TDVSIRDNFGKATISTAFGIDVGAIYRPSSWLRFAIVAKDINQPTFDTPGGGEIKLGP
                     QVRGGVAVNPYSSLTLTADVDATSNKTFVPGIKSQILSLGAEQTIFSEFLSFRVGAFK
                     NMKDAGTPFTPTAGLGLRIFALRIDVGGGYDFREEGALVSGSVSLTF"
     gene            3050..3550
                     /locus_tag="VLL06_11400"
     CDS             3050..3550
                     /locus_tag="VLL06_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31615.1"
                     /translation="MFRPQAIAVLPPMASQYDNAREDIQGVLSEALNKTAKIERVVSP
                     ENVTDIFQASKEAFDALVFYFSRLEMTGQSDKDSAVKLGKALNVDSFLVVRVNSWEYM
                     RKEGDNVGRVGLSLRLVDATTGTTVWKARHERSSSYMFYRPNLKDVAKELADEMIKAM
                     PPQAKQ"
     gene            complement(3656..>3951)
                     /locus_tag="VLL06_11405"
     CDS             complement(3656..>3951)
                     /locus_tag="VLL06_11405"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HSS31616.1"
                     /translation="CVSLTGITGAKLHNVADVGKNVEKIRKVTRVPVTVGFGVATPGD
                     AADVAAIADGVIVGSAIVKQIAAHQQKPEMVKHVAEFVRSLKTAMRTAQPKTR"
BASE COUNT          868 a         1092 c         1074 g          917 t
ORIGIN      
        1 cgcgagggca cggtgaccga actggccaag aatctttcga ctgggcaatt ggacgacctc
       61 agccggctgg tcaaggtgaa gcagtcaccg gtcgatgatc cgaccggccc ggcggtcgag
      121 acagaaaaac ccggcatcat cctgggcaag gaattggcga tgcgactcgg cgtctttgtc
      181 ggagacacgg tcaatgtcgt gtcccctcca gccggtccga tcagcgcgat cggcatggta
      241 ccgaagatca ggaccttcgc attggtcgcg ctctttcaat ctggtatgta cgagtacgat
      301 tcgtcattgg cttacatcga tctcgcggag gcgcaaaagt ttttcaacat gggacagacc
      361 gtgacaggca ttgaaatcaa agtcgaggac gtcttccacg cggcggagac ggcccgttcc
      421 gtggagcagt cgctcggttt cgcctatggc gcgcgcgatt ggatgcagat gaatcgtaat
      481 ctcttctcgg cgctcaagct cgagaagacg atgatgtttc tgttgttagt actgatcacg
      541 atcgtggcat ccttcaatat cgtgagcacc ctcaccatga tcgtcaccga aaaacaaaag
      601 gaaattgcga tcctcaaggc catgggcgca acacgcaaaa gcattcggcg cattttcatg
      661 ttgaacggac tgatcatcgg cctgtccggg accgccatcg gcattccgct cggctacgcc
      721 tttctctggc tgatccagac gttctggacc tttgacccca cggtgtatta catctcgcgg
      781 atccccgtcc acgtgcaagc actcgatgtc atgcttgtgg cgggttccgc cattgtcatt
      841 agctttgccg ccacggtttt tccttcgctc caggcagcca agcttgaacc ggtggctgcg
      901 ctgcgctacg aatgatcatg caggcctccc cttcgttatt cgttcagtct ggagctccgg
      961 cgtgatcacc gtcaccaatc tgtacaaatc gttccccatg ggcggacaaa ccctcacggt
     1021 tctgaagggg gtcaatctac agattcagcg gggggagttg attgcgattg tcggagcctc
     1081 gggagccgga aagagcacac tgcttcatat cattgggaca ctcgatcgtc ccaccacagg
     1141 tacggttcac tttgactccc aggatctctt tcaactggcg gaaggcgaac aggcggattt
     1201 tcggaatcgc cgtatcgggt ttgtcttcca gtttcatcat ctcctgccgg agttcactgc
     1261 gctcgaaaat gcctgtatgc cggcattaat ccagcggcac aacctggcag atatcgaacc
     1321 ggaagccacc gccttgttgc aagaggtcgg gctgggagac cggctacatc acaagcctgg
     1381 tgagttgtct ggcggtgagc agcagcgcgt ggcagtcgca cgagccctct tgcagaaacc
     1441 cgatctcgtg ttggcggatg agccgacggg aaacttagat acacacacgg gcgaggcgct
     1501 ctttgcgatg atgcgtgagc tgaacaaagc ccgcggaacg accttcgtga tcgtcaccca
     1561 taacgacaag ctatccgccc aagcggaccg tattgtccat atgcaggacg gccaaatcgt
     1621 gtaagcgaaa ttgtggcatc ctgtcgtatt caccgaaaaa gtcccctgcc gagtccaccc
     1681 ttcgcataca tacaagagga ttgatcgtct agccttgacg agtgctatgt catccaatag
     1741 actcaggaag ccatttcgac tagcacggag ctgtccctct atgtcgtgtt atcgcactct
     1801 ttgtgccgca gcgatcatca ttggccttat gattccggcg caggcactgg ccttagaatt
     1861 cgtcacagtg ggtcctcgtg caatgggcat gggcgaagcc ggtgtggcgg tcaccacaga
     1921 tgccttggcc acctattgga accctgccgg gcttgcgatg acccagacgg tggatgttcg
     1981 catccaaggc agtggacaag tcattgatcg tctcggcatc gccaatgcgg tccacgatct
     2041 cgaagaattc aatacaagtg atagctcggc cgctaatctt gctaaagctc aaaatatcgc
     2101 caatcgcatc aatcgacctg gcgctaccgt ttcggtcaat ggctctgcag gtctctatgt
     2161 caagggtcac ctgggggaac atgcctttgg cctgaacgtc tcggatgttg cgaccggcgg
     2221 gggatttgtt tctacgccta ttcaagcgac tcaacctggt ggagcgggca ctcccatcac
     2281 tctggcagga caaatggctc tccttggttt agaggccagg caggtggcat tctcatatgc
     2341 ctatgccttt tccgataaaa cattttcttt cggtgtgacg ggaaaaattc ttcaaggagc
     2401 cgcctacagt gggagcacaa ctctcaccgg aggaaccgat gtttcaatta gagacaactt
     2461 tgggaaagcc acgatctcca cagcttttgg tatcgatgtg ggtgccatct atcgcccatc
     2521 ctcatggctg cgctttgcca ttgtagctaa agacattaat caaccgacct ttgatacgcc
     2581 aggcggagga gagatcaagc tgggacccca ggttcgagga ggtgtagcgg tcaaccccta
     2641 ttcttcgctg acgttgacgg cagatgtgga tgcgacctcg aataagacgt ttgttccagg
     2701 catcaagagc cagatcttga gtctgggagc cgaacagacc atcttctctg aattcctgtc
     2761 attccgcgtc ggcgccttca agaacatgaa ggatgcgggg acaccattca cccctactgc
     2821 aggattgggc ttacggatct ttgctctccg tattgatgta ggcggcgggt atgacttcag
     2881 agaagagggc gcgttggtat ccggctctgt ctctttgaca ttctaatggt atactaagcc
     2941 catgtttata tggcttcata tcagacgtgc gatccccctc tgccttctgc ttctcgttgt
     3001 cgccagctgc ggcggtttac aagaagtgtg ggaaggacct ggggcaaaaa tgttcagacc
     3061 tcaagccatt gctgtgcttc ctccgatggc gagtcaatat gacaacgcgc gcgaggatat
     3121 ccaaggcgtc ttgtcagaag ccctgaacaa gaccgccaaa attgagcgag tggtgtctcc
     3181 tgagaatgtc acggacatct tccaggcttc aaaagaagcg ttcgatgcgc tcgtgtttta
     3241 cttctcacgt ctggaaatga ccggtcaatc cgacaaggac tccgccgtga agttgggaaa
     3301 ggcgctgaac gtagactcgt tcttagttgt acgggtgaac tcttgggaat acatgcgcaa
     3361 ggaaggtgat aacgtcggcc gagtgggcct cagcctccgt ctggtcgatg ccaccacagg
     3421 caccacggtg tggaaggcgc ggcatgaacg gtcgagcagc tacatgttct accggccgaa
     3481 tttgaaggac gtcgcaaaag agctggcaga tgagatgatc aaggccatgc ctcctcaagc
     3541 caaacagtaa tcctcaccca aactgattgt gattcagggc gcttgaacac ctgaccctcc
     3601 tcaggtcgca cagcgcgttg ttttctttcc aagaagagaa gcctggatga gtcgcctagc
     3661 gtgtttttgg ctgagcggtc ctcatagcgg ttttcaacga gcggacaaat tcggccacat
     3721 gcttcaccat ctctggtttc tgttggtgtg cggcaatctg cttgacgatc gcgctgccga
     3781 caatcacccc gtcggcaatg gccgcaacat cggcagcatc ccccggcgtc gcaaccccaa
     3841 aacccaccgt gaccggaacg cgggtgactt ttctgatttt ctcgacgttc ttccccacat
     3901 cggccacgtt gtgcagtttc gcgccagtga ttccagtgag cgacacgcaa t
//
LOCUS       DATIPF010000460         3153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_792200, whole genome shotgun sequence.
ACCESSION   DATIPF010000460 DATIPF010000000
VERSION     DATIPF010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792200"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VLL06_11410"
     CDS             <1..494
                     /locus_tag="VLL06_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31617.1"
                     /translation="HVLSRWPLTALVIPLALGLGMGGIGSAEESLYDPDVVLVMKDKG
                     FHVIKGAQSGNTLSKPAFSLPVGRDLVIQLNNEDEVAHEFVSPLLMKAEDLQISGRAT
                     LVYTHTAFGVRVEPHDSVMLRFDIPDAGFDQFHFWCNIHGKFVGDTMRGEIFILNAKP
                     SSK"
     gene            639..1220
                     /locus_tag="VLL06_11415"
     CDS             639..1220
                     /locus_tag="VLL06_11415"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HSS31618.1"
                     /translation="MFKRTAGRILGGLCAVMVAGVAVDAAQAGETAGIPPEMVAEYIH
                     AVVQADRTLYSTHVVERLHELKIPVAVEEWKARGTLPLPAQMLQMAGQEIQGLGLGLR
                     IRLASLGPIYKKNGPADQFERAGLEAVAKNPRKPYTGIITEGDKRFFKAIFADRAVSI
                     TCVACHNSHELSTKRDYKLYDVMGGIIISFPVQ"
     gene            complement(1322..1849)
                     /gene="sodC"
                     /locus_tag="VLL06_11420"
     CDS             complement(1322..1849)
                     /gene="sodC"
                     /locus_tag="VLL06_11420"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HSS31619.1"
                     /translation="MKKQIPFFLFLGALAVSAAAQADTGVQMNLVDENGVGKGIGQVT
                     ISETQYGLEFSPAITGLSPGLHGFHVHENPSCEPKEKDGKKMPALAAGGHYDPAGSKR
                     HSAPWRDGHLGDLPALSADANGNASPVLAPRVKMADLMGRALMIHAGGDNYADQPAPL
                     GGGGIRWACGVIQAP"
     gene            2217..>3153
                     /locus_tag="VLL06_11425"
     CDS             2217..>3153
                     /locus_tag="VLL06_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSS31620.1"
                     /translation="MIRIALLLSTLAFSVVALLSLPSWAYRDYFTTEQKALLEKIQTL
                     RVEAIALTDQGAVDAAPITELVARRIGELDFTVVREAGKPHDAVVKVKCEQRKTWEGT
                     TAAGGDADLPDAPSRLWKGPACQMTYLLGDMKVKWQKEVRTEFEDAAQAAQSANAGDP
                     GIYALGKLRDVLETYELPLLLAAEWGQSARLLRLLDRSDTSKARKLKIISLLGEMQAD
                     EALPKLKEALRDRDLAKHAIGAMSNLGKEGIPLFVETMNTSPDLDVQATAAKGLGQLG
                     GLHGDASVVPPLRAKLKDPKTDWSVLTEVAWALGKI"
BASE COUNT          725 a          814 c          898 g          716 t
ORIGIN      
        1 aacacgtgct atcacgatgg ccattgacgg cgttagtgat cccgctcgcg ctagggctag
       61 ggatgggtgg aataggttcg gccgaggagt cgctgtatga ccccgatgtg gtccttgtga
      121 tgaaggacaa aggttttcac gtgatcaaag gtgcacaatc agggaatacg ctgtctaagc
      181 cagccttctc gttaccagtc gggagggatc tcgtcatcca gctcaacaat gaggacgagg
      241 tggcgcatga gttcgtctcg ccgttgctca tgaaggcgga ggaccttcag atctcaggcc
      301 gggcgaccct cgtgtatacc cataccgcct tcggggttcg ggttgagccg cacgacagcg
      361 tgatgcttcg ctttgatatc ccggacgccg gctttgatca gtttcatttt tggtgcaata
      421 tccatgggaa gtttgttggt gacaccatgc gaggggagat ctttatcctg aatgcgaaac
      481 catcatccaa gtaaccggac ttcgagatgt gcgagtgcgg attggcagaa tagaggaagt
      541 ctggaaacag gacggagtgt gatggatgcc atggcaccga tgacgatgtg cttgtctgaa
      601 gcaggttctg cctcagagcg ctgatgaaga ggcttaccat gttcaagaga acagcaggac
      661 ggatccttgg tggactctgc gccgtcatgg ttgccggtgt cgcagtcgat gccgcgcagg
      721 cgggcgagac ggcgggcatt ccacctgaaa tggtggcaga gtacattcat gcggtcgttc
      781 aggccgatcg gaccctctat tccacccatg tcgtggagcg gctgcacgaa ctcaaaatcc
      841 ctgtcgcggt ggaagaatgg aaagcgaggg ggacgttgcc tcttcccgcg caaatgctac
      901 agatggctgg tcaagaaatt caggggctcg gactcggact tcgcatccgg ttggccagct
      961 tggggccaat ttacaaaaaa aacgggcctg cggatcagtt cgaacgggcg ggcctggaag
     1021 cggtggcaaa gaatcccagg aagccctata cgggaatcat taccgaaggt gacaaacgtt
     1081 tttttaaggc gatatttgcg gaccgggccg tttccatcac ctgtgtcgcc tgccataaca
     1141 gccatgagct gagcacaaag cgggattaca agctctacga tgtgatgggg ggaattatta
     1201 tttcgtttcc agtccaataa tcccattccg cgcataccgt aatgtcctgg tgtctccacc
     1261 gggactcgag gaagaaaccg gacacctcga ccattatcgg ctcagcgaat ccacttacaa
     1321 atcagggggc ttgaatgaca ccacaagccc aacgtatccc tccaccgccc aatggagccg
     1381 gttgatccgc gtaattatct cctcctgcat ggatcatgag cgcgcggccc ataaggtcgg
     1441 ccatcttcac acgtggggcc aatacaggac tggcattgcc atttgcatca gcggacaatg
     1501 ctggcaaatc cccaaggtga ccgtcgcgcc agggcgcact atggcgtttg ctgcctgctg
     1561 gatcatagtg gccgccagca gctaatgcag gcattttttt gccgtctttt tccttcggct
     1621 cgcaactcgg gttttcatgc acatggaacc catgtaaccc cggtgataga ccggtgatgg
     1681 cggggctgaa ctcgaggccg tattgcgttt cactgattgt gacttgccca atccctttcc
     1741 cgacaccatt ctcatcaaca agattcatct gaaccccagt atctgcttgt gcagcagcac
     1801 ttacagcaag tgcgcccagg aacaaaaaaa atggaatctg ctttttcatg gcgttcctcc
     1861 gttttgtcca atctgtttca tgatgaactg ccctgtcgat gagcggcgcc tattctcctc
     1921 aattcaaaat cgcatgtcaa tgagacacta tcggaattat ttgtttttga actagcggaa
     1981 acgctagata ggtgatgggg agggctgacc ctatcctcaa aaggtatttc ccctgatccg
     2041 gggtaactat tgccggagga ggttgtgatt acgagcaata ggtacccctc catatttttt
     2101 atatgtccca ctattgcaga agccgtcaaa caaagagcta cggtagcctc aacctccgat
     2161 gacgggtata atcaggtcgc atgcgtcatc atcgcccgta tctggttagg atgtgcatga
     2221 tacgcattgc cctgctgctc tcaacactgg cttttagtgt tgtggcactt ctctctcttc
     2281 cttcatgggc ctaccgagac tacttcacga cggaacagaa agcgctactg gagaagattc
     2341 aaaccctgcg tgtcgaggcg attgccctta cagaccaggg ggccgttgac gctgcgccga
     2401 ttactgagct tgtcgctcgc aggatcggcg aattggattt cacggttgta cgagaggccg
     2461 gtaaacccca tgacgctgtg gtcaaagtca aatgcgaaca gcggaaaaca tgggaaggca
     2521 ccactgccgc tgggggggat gcggatttgc ccgatgctcc gtcgcgactg tggaaaggcc
     2581 ccgcctgtca gatgacctat cttctcggtg acatgaaggt caagtggcag aaagaagtcc
     2641 gaaccgagtt tgaagatgcg gcgcaagccg cgcaatcggc caatgcagga gatcctggaa
     2701 tctatgccct gggcaaattg agggatgtgc tcgagacata cgaacttccc ttgttgctgg
     2761 ccgctgaatg gggtcagtcg gcgcggcttc tcaggttgct tgatcgatcc gatacatcga
     2821 aggccagaaa gttgaaaatt atttctctct taggcgagat gcaagccgac gaggccttac
     2881 cgaaattgaa agaggcgctg agagatcgtg acctggcgaa acacgcgatt ggggcgatga
     2941 gcaatcttgg caaagagggt attcctctgt tcgttgaaac catgaatacg tcaccggacc
     3001 tcgatgtgca agccacggca gccaaggggt tgggccaact cggcggactg cacggggatg
     3061 cctcagtcgt gccgccgctg cgggccaagc tgaaggatcc caagacagac tggtccgtgc
     3121 tcacggaagt ggcctgggcc ttggggaaaa ttc
//
LOCUS       DATIPF010000461         4687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1313377, whole genome shotgun sequence.
ACCESSION   DATIPF010000461 DATIPF010000000
VERSION     DATIPF010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313377"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..798)
                     /locus_tag="VLL06_11430"
     CDS             complement(208..798)
                     /locus_tag="VLL06_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HSS31621.1"
                     /translation="MWLVLLWLGAMAWLPTMVQAEPARWNLDPEHSTIEFRVAHMVVS
                     KTTGRFMDYTGFIDMDAEDGTVTAIEATIKAESVNTNHVKRDIHLRNAEFLDVKLYPT
                     MTFKMKSYKKTAEGFTAVGDLTLHGVTKDVTLAGRYNGATKDPWGNDRAGFSAEGKLN
                     RKDFGMVWNKTLDSGGLVVGDEVQIRLDIECIKAKP"
     gene            complement(875..1708)
                     /locus_tag="VLL06_11435"
     CDS             complement(875..1708)
                     /locus_tag="VLL06_11435"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HSS31622.1"
                     /translation="MGDPQTGKPVTLTIVLGGVTAGTTPVVATCESIAFQTQTVTLEP
                     DGESMTLKGTVTLEPILMSRTTVAPRAARVQVTFARTRQDKLERFMQRVVYVTMDRSE
                     PANENESSESPPVRPEESQYEDVILDEVQSGVEPVATGAVAEEDLVPFATPGEGKAYW
                     QQVSHLISRSWARQVRGVRHRPSSETVRVRFKMFPNGRAQLVEIEKGSGTREIDEAGI
                     HAVVNAQPFLPFPSELGEDPVDVHVRMRTGSRGRSPREVLSVGSRSTTKSGASSQPSR
                     R"
     gene            1969..3003
                     /gene="tdh"
                     /locus_tag="VLL06_11440"
     CDS             1969..3003
                     /gene="tdh"
                     /locus_tag="VLL06_11440"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HSS31623.1"
                     /translation="MRALVKTSAQPGLTATDRPDPKPGPADAIIRVTATSLCGTDAHI
                     YNWDPWAHSRVHPPRIIGHEMCGEVVEVGSDVTLVKMGDYVAAESHLTCGHCFQCRTG
                     QAHVCKNYRILGVDHDGSFAEYMALPASVLWKTSPAIPRELACLQEPLGNAVDAALVE
                     NLTGQTVLITGCGPTGLFAAAVARTAGAAMIIATDVSAYRLDLAKQVGVDHVLNAKTE
                     TADRIAAAIREITAGEGVDASLEMSGNPTALHHAFSSVKNGGRVTLFGIPTGPIMFDL
                     ANEVIFKGIRVYGITGRRLFKTWYRLTGLFQAGLNIKPVITHTFPLKDFTRGFELINS
                     GQCGKVVLIP"
     gene            3105..4295
                     /locus_tag="VLL06_11445"
     CDS             3105..4295
                     /locus_tag="VLL06_11445"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HSS31624.1"
                     /translation="MAYDSLKTTVSARLAEIRAAGLYKSERQILGPQGAEIRVAQGEV
                     LNLCANNYLGLANHPDIRQAAAAGLQEHGYGMASVRFICGTQDLHAQLEQTLSTFLGT
                     EDTILYSSCFDANGGLFEVLLGEHDAVISDALNHASLIDGIRLCKATRLRYAHSDMAD
                     LEAKLRDATSYRLRLIATDGVFSMDGDLAKLDRIAELADRYDAAVIVDDSHATGVLGR
                     NGRGTPDHFGVADRIEIVTSTLGKTLGGAAGGFTSGRKELVELLRQRSRPYLFSNSLP
                     PGIAAAARRAVILVAQGDQLRIRLRENAIFFRAQLTALGFRLIPGEHPIIPIMLGEAS
                     LATRMAERLLEEGIYVVGFSYPVVPEGQARIRVQMSAAHQREQLERASAAFATVGREL
                     GVIP"
     gene            complement(4362..>4687)
                     /locus_tag="VLL06_11450"
     CDS             complement(4362..>4687)
                     /locus_tag="VLL06_11450"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HSS31625.1"
                     /translation="KPGPYLVVPFLPPFTVRDFAGYVGDIFLNPINWLVVRIIEVDGV
                     PSVVAHKNRMTTSIIQVGARVGEIVNERSRNLEKFQGVEEATLDLYTAVRNAYLQKRA
                     QAIRE"
BASE COUNT          962 a         1453 c         1278 g          994 t
ORIGIN      
        1 ccaacaccat cgccttcatc agtgattccc ctcaggaaaa ttggccgatg gtgaaccgcg
       61 cgcaagcgga cgaacacaac acaggatgct caaaaaggcc gtccagcaag gccgcagccg
      121 aagaaagcac cggaggcgta gcctctgggc tacgttgagg atgctttcga ggggagaacg
      181 cagctggcgg gctttttcag catcctgcta gggtttggct tttatacatt caatatccag
      241 ccgaatctgg acctcgtcgc cgacgacgag tccaccgcta tccaatgtct tgttccagac
      301 cataccgaag tctttacggt tgagctttcc ctctgcgctg aagccggccc tgtcattgcc
      361 ccagggatcc ttcgtcgccc cattgtagcg gccagccagg gtgacatcct tcgtcacccc
      421 atgcagcgtg agatctccca cagcggtaaa tccttctgcg gttttcttgt agctcttcat
      481 cttgaaggtc atcgtcggat acagtttcac atctaaaaat tcggcatttc gcaagtggat
      541 gtctcgtttg acgtgattcg tattcaccga ctcggccttg atcgtggcct caatcgctgt
      601 cacggtcccg tcttcggcat ccatgtcgat aaacccggta tagtccatga atcggcctgt
      661 ggttttagag acgaccatgt gcgccacgcg aaactcgatg gttgaatgtt cgggatccag
      721 gttccaacgg gctggttctg cctgaaccat tgttggaagc cacgccattg ctccgagcca
      781 taaaagcacc aaccacatct gccctctcct cttcagcatg aatgactcct cttgggtgaa
      841 tcgtatggat ggactgggca tggatcgatt ggacctatct cctcgaaggc tggctcgatg
      901 caccagactt ggtggtggac cggctcccga ctgatagcac ctcgcgagga gaccgtcctc
      961 gactcccggt tcgcatccgg acgtggacat ctaccggatc ctcccccaac tcactgggga
     1021 acggcaggaa cggttgggca ttgaccaccg catggatgcc ggcttcgtca atttctcgag
     1081 taccggagcc tttctcaatt tcgaccaact gggctcggcc gttgggaaac atcttaaatc
     1141 ggaccctcac cgtttcactg ctgggccgat gccggacccc gcggacctgg cgagcccaac
     1201 tccgactaat gaggtgactc acctgttgcc agtaagcctt tccctcccct ggggtggcaa
     1261 aggggacgag atcttcctcg gctacggcgc ccgttgcaac cggttccacg cccgattgca
     1321 cttcatccaa gatgacatcc tcgtactggg attcctccgg acgaacgggc ggcgactcgc
     1381 tactctcatt ctcattggcc ggttccgagc gatccatcgt cacatatacg acacgctgca
     1441 tgaaccgttc aagtttgtcc tgacgggttc gagcaaacgt cacttggact cgcgccgccc
     1501 tcggtgccac ggtggttcga ctcatgagga tcggttcgag cgtcaccgtc cccttcaacg
     1561 tcatcgactc tccatccggc tcaagggtga ccgtttgggt ctgaaatgca atcgattcgc
     1621 aggtcgccac tacgggagtc gttcccgctg tcactcctcc aagaacaatc gtcagcgtga
     1681 caggcttccc ggtttgcgga tcccccatcg actccgcctg actcaactcg aattgcgccc
     1741 accgtttcgt gggaccggac gaggaagccg tctgtggcga tgattcggaa tgcgctggta
     1801 cgatgaggca aggagagacg aatgccacac aacatatgcc aactaaaatc aggcttcggt
     1861 ggggcagagc cttcatagat tgcatcctac cgatctcgat gccccttgcg caacccgttt
     1921 ttccctgcaa gggtaggcgg aatcgcccac ctggtataca atgaagtcat gcgtgcactc
     1981 gtcaagactt cggcacaacc gggtttgacc gccaccgata gaccagaccc gaagccgggc
     2041 cctgccgatg ccatcatccg tgtgacagcc acctctctct gcggaacaga tgcccatatc
     2101 tataattggg atccttgggc acacagccgc gtccatcccc ccagaattat cggccatgaa
     2161 atgtgcggcg aagttgtgga ggtcggttcg gatgtgacgc tcgtcaagat gggcgattac
     2221 gtcgccgccg aatcgcacct cacatgtggt cactgtttcc aatgtcgcac cggacaggcg
     2281 cacgtgtgca agaattaccg catcctcggc gtcgatcacg acggatcgtt cgcggaatac
     2341 atggccttgc ctgcatctgt gctctggaag acttcgcccg cgattccacg agaactcgcc
     2401 tgtctccaag aaccgctggg caatgccgtc gacgctgcct tagtcgagaa cctcacagga
     2461 cagacggtct tgatcaccgg ctgcggaccg accggactct tcgcggccgc cgtcgcacga
     2521 acggccggtg ccgccatgat catcgccacg gatgtcagtg cctaccgtct ggacctcgcc
     2581 aaacaggtcg gtgttgatca cgtgctcaat gccaagactg agacagcgga ccgcatcgcc
     2641 gccgccattc gcgagatcac ggccggagaa ggcgtcgacg cctcgctcga aatgtcaggg
     2701 aacccgacgg cattgcatca cgccttcagt tccgtcaaga acggtggccg cgtgacactc
     2761 ttcggtattc caaccggccc gatcatgttc gacctcgcga atgaagttat ttttaaggga
     2821 attcgcgtct acggcattac cggccgccgg ctcttcaaaa cgtggtatcg cttgaccggc
     2881 ctcttccagg cgggactcaa catcaagccg gtcatcaccc atacgtttcc cctgaaagac
     2941 ttcacacggg ggttcgagtt gatcaattcg ggccagtgtg ggaaagtggt cttgattccg
     3001 taaaagctgt ccgccgtcag cgatcagacc agatttcatg cgaaatgtac ttcctctgcc
     3061 tccatacgag gctgatcgct gacggctgtt gctcagtgaa gacgatggcc tacgactcac
     3121 tcaagacaac tgtatcagcg cgactcgctg aaatccgcgc ggcaggcctc tataaatctg
     3181 agcggcagat cctcggtccg cagggggccg agattcgtgt ggcgcaaggc gaggtcctta
     3241 atctctgtgc caacaactat ctgggactcg ccaaccatcc cgatatccgc caggccgctg
     3301 ccgccggact gcaagagcat ggctacggca tggcctccgt ccggttcatc tgcggcacgc
     3361 aggatctcca cgcacaactc gagcagacac tcagcacgtt cctgggcacc gaggatacga
     3421 tcctctacag ttcctgcttc gatgccaacg gcggactctt tgaagtgctc ctcggcgaac
     3481 acgacgcggt catcagcgac gccttgaacc acgccagcct gatcgatggc atccggctct
     3541 gcaaagccac caggctccgc tatgcgcact ccgatatggc ggacttggaa gccaagctgc
     3601 gcgatgcgac atcctatcgc cttcgcctga tcgcaacgga cggggtcttc tccatggatg
     3661 gcgacctcgc gaaactcgat cggatcgccg agctggcgga tcgatacgat gccgcggtga
     3721 tcgtggacga cagtcacgcg accggcgtgc ttggacgcaa cggacgcggc acgccggacc
     3781 atttcggcgt cgcagaccgg atcgagatcg tcacgagcac gctgggaaaa acattgggcg
     3841 gcgccgcagg tgggtttacg tcaggacgga aggaactcgt cgagctgttg cggcagcggt
     3901 cgagacccta cctgttttcg aattcactcc ccccgggcat cgccgcggcg gctcgtcggg
     3961 ctgtgatcct cgtggcccaa ggcgaccaat tacgaatcag actgcgcgag aacgcgatct
     4021 tcttccgtgc gcaactcacc gcgcttggat tccgactcat tcccggcgag catcccatca
     4081 tccccatcat gttgggtgaa gcgtcactcg ccaccagaat ggccgagcgg ctattggagg
     4141 aagggatcta tgtcgtgggg ttcagttacc cggtagttcc ggaaggtcag gcgcggattc
     4201 gtgtgcagat gtcggcagcc caccagcgcg aacagctgga gcgagccagc gccgcctttg
     4261 caacagtcgg tcgggaacta ggcgtcatcc cgtgaagcgt gtgtctggtc tatttggttc
     4321 atctggtttc tctcgttcac aaccaaacaa accggacttc tctattccct gattgcctgc
     4381 gcgcgcttct gtagatacgc gttgcgcacg gccgtataga gatccaacgt cgcttcttcg
     4441 accccctgga acttttcgag attccgcgac cgttcattga cgatctcccc cacgcgcgcc
     4501 ccaacttgga tgatgctcgt tgtcatccgg ttcttgtggg cgaccacgga agggacaccg
     4561 tcgacttcaa tgatccgtac gaccagccag ttgatcggat tgagaaatat gtcgccgaca
     4621 tatcccgcaa aatcccgaac ggtgaagggc ggaagaaacg gcacgacaag ataggggccc
     4681 ggcttca
//
LOCUS       DATIPF010000462         4645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1590558, whole genome shotgun sequence.
ACCESSION   DATIPF010000462 DATIPF010000000
VERSION     DATIPF010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590558"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..382
                     /locus_tag="VLL06_11455"
     CDS             119..382
                     /locus_tag="VLL06_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31626.1"
                     /translation="MVYTSESIASRIAWCQWQKTQTSAQPDIQGWHAEEEGLRDALLN
                     RDHTNQYRDYPRGVFERYLLGLQDGRAMIRIEGLVHHRATSHL"
     gene            663..1067
                     /locus_tag="VLL06_11460"
     CDS             663..1067
                     /locus_tag="VLL06_11460"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSS31627.1"
                     /translation="MNIATLFSRKHDCPVKVIDPRRCARRMVRFSTTVAAKSGLADGV
                     VTNISETGCDLRLVTSSFPRQSLFLKLRSQDALALTIYPYDGTAAFQITLAVIRWVRR
                     KWAGVEFVKLTQEAKEKLQQLCRDQVLLVVTG"
     gene            1196..2110
                     /locus_tag="VLL06_11465"
     CDS             1196..2110
                     /locus_tag="VLL06_11465"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HSS31628.1"
                     /translation="MANPILVTGAAGRVGGIGRTVTGLLLKQGKAVRAMVRNEDERAR
                     ALRDMGAEVVVGDLLELDSMHRAIAGCDTMYLGMSVSDTYLTATVNAAAVAKHHGVKA
                     LINMSQMTLAQMSITETTPSPQHKLHWLAEQALNWSGLPVVHVRPTVLLEGFFLIVTP
                     ESVSESNQIRLPFGEGKTSPVAVEDVARVVAALLAKPQPHIGKIYHLTGPQSENMDAI
                     AQEYSKALGRTITFVDIPVEAWRDGLLKRGLPVHLVNHLATMADLHRAGRYDRMSGDV
                     LMLTGQTPLSVQEFVRRNAATFTRSARD"
     gene            2216..2857
                     /gene="tmpT"
                     /locus_tag="VLL06_11470"
     CDS             2216..2857
                     /gene="tmpT"
                     /locus_tag="VLL06_11470"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HSS31629.1"
                     /translation="MMDPSFWHDRWEKNEIAFDESKANPVLIKHVNELSLAKGSRVFV
                     PLCGKTRDISWLLSKGYRVAGAELSQIAIEQLFMELGLQPEISTVGEVEQWSANHVDI
                     FIGDIFALSRKMLGPVDTIYDRAALVAFPEDMRNRYTAHLTGITHKAPQLLICYDYDQ
                     SLMQGPPFSIHNEEVHRHYAGSYELTLIASTDVSGGLKGKCAAKENVWLLKSK"
     gene            complement(2980..3480)
                     /locus_tag="VLL06_11475"
     CDS             complement(2980..3480)
                     /locus_tag="VLL06_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31630.1"
                     /translation="MDVVSHGLWGSIAFGRKSRSSFWLAFVTGTAPDIFSFGILWVAA
                     ILGLSEQPDFSHGTPPESTIPQYVHHLYNVTHSFIIFLAVFFLVWFLLKRPLWELGAW
                     GLHVLVDIPTHSYAFFPTPVFWPLFDWKFDGWQWMTPSILIPNFVFLLLVYAWYFWQS
                     YRTKGR"
     gene            3888..4319
                     /locus_tag="VLL06_11480"
     CDS             3888..4319
                     /locus_tag="VLL06_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSS31631.1"
                     /translation="MKQATHPRFTKLPYLIFTLLLTIGLCVLSGTLMAQEGKVTPLMS
                     EVLTDISGKEGLMITVEYAPGGSSPIHRHNAHAFVYVLEGSIVMQVKGGKEVTLTPGQ
                     TYYENPNDVHVVSRNASTTAPAKFLVVLVKDKGAPVVVPVK"
     gene            4432..>4645
                     /locus_tag="VLL06_11485"
     CDS             4432..>4645
                     /locus_tag="VLL06_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSS31632.1"
                     /translation="MKIVVIGGSGLIGTKLVNRLRKHGHEVVAASPKSGVNTITGEGL
                     AEALAGAQVVVDVANSPSFEDKAVLKF"
BASE COUNT         1113 a         1244 c         1298 g          990 t
ORIGIN      
        1 ccagagagga tgcaatgagt agttccctgg tatctatctt gcgccttatt agagagaagg
       61 ggcgtagtag gtcggcaaca gagtccacac ttgtacgacc caagaatgga ggggagccat
      121 ggtgtataca agtgaatcca tcgcatcccg cattgcatgg tgccaatggc agaaaactca
      181 gacgagtgca cagcctgaca ttcaagggtg gcatgccgaa gaggagggtc tcagggacgc
      241 gctcctcaat agagatcata cgaaccagta tcgggattat ccgcgaggtg tgttcgagcg
      301 atatctgctg ggtctccagg acggccgagc catgattcgt attgaagggc tggtccacca
      361 tcgcgcaaca tcccatctct agtgccataa gcgtcttcga ggtcagcaga tcgcattgtc
      421 cgtagctgtg tcctgagatc agaaatcgaa agtcggacga caagacctga ccccttgggt
      481 tcagttactc gcctcgtttt ctcaatcttt gtctctgctc tgaccccccc caatccaact
      541 ctccacgtgc ttcctccacc tcactgcacg accttctgat ttgttataat ctacgtactt
      601 tcgatatgag ggttggtcgt agattcgcag gtgcgcagca actacggtaa aggaggttcc
      661 caatgaacat tgccacgttg ttttctcgca aacacgattg ccccgtgaaa gtgatcgatc
      721 cccgacgatg tgcgcgaaga atggtgcgct tttctacgac cgtcgcagcc aagagcggtc
      781 tcgcagatgg agtggtcacg aatatttccg agacggggtg tgacctgcga ctggtcacct
      841 cctcctttcc caggcaatcc ctcttcttaa agctccgttc gcaagatgca ctcgctctga
      901 cgatctatcc atatgatggg actgctgcat ttcagattac cctggcggtg atccgatggg
      961 tcaggaggaa atgggctggt gttgaatttg tgaagctgac tcaggaagcc aaggagaaac
     1021 tccagcaact ctgtcgcgac caagtcctcc tcgtcgtgac tggttaggta ccgacgatgc
     1081 cggcccccgg ccggtctgtc gagacgccgg gggtggcatc gccagagcta cagatatata
     1141 cgtacaacca tagtatggcg cgtcttaacc tgatgcaccc atcgaaggaa tgaacatggc
     1201 aaatccaatt ctggtcaccg gcgccgcggg ccgcgtcggc gggatcgggc gcacggtcac
     1261 cgggttgcta ttgaagcaag gcaaggcggt gcgcgcgatg gtgcgaaatg aagacgaacg
     1321 cgcgcgggcg ttgcgcgaca tgggtgcgga ggtcgtagtc ggcgacctgc tcgagctcga
     1381 ttcgatgcac agggcaattg ccggctgcga cacgatgtac ctcggcatgt ccgtttcgga
     1441 cacctacctg actgcgacgg tcaatgcggc ggcggtggcg aagcaccacg gcgtgaaggc
     1501 gttgatcaac atgtcgcaga tgacgctcgc gcagatgagt attaccgaaa ccaccccgag
     1561 cccgcagcac aagctgcact ggctcgccga gcaggcgctg aactggtccg gtctgccggt
     1621 cgtgcacgtg cgcccgactg tgcttctcga aggcttcttc ttgatcgtca ctccagagtc
     1681 ggtcagcgag tcgaatcaaa tcaggttacc gttcggcgag ggcaagactt cgccggtcgc
     1741 ggtggaagac gtcgctcgtg tggtcgccgc gctgctcgcg aagcctcagc cgcacatcgg
     1801 gaagatctat cacctgaccg gtccgcagtc cgagaacatg gatgctattg cgcaggagta
     1861 ttcgaaagcg ctcggccgca caattacctt tgtggacatt ccggtcgagg cgtggcggga
     1921 cgggctgcta aagcgcggct tgccggttca cctggtgaac cacctcgcga caatggccga
     1981 tctgcaccgc gcaggacgct acgaccggat gtcgggcgac gtgctcatgc tcacgggcca
     2041 aacgcccctg agcgtgcagg agttcgtccg gagaaatgcg gcgaccttca ccaggtcggc
     2101 aagggattga tagggacgat gttgcccagc cggcggcgat caccgcagcc ataaactgct
     2161 ctcattgcca ggcaacagct caaatatgct caattgtcat gtgtaatagg aagccatgat
     2221 ggacccaagc ttttggcatg acaggtggga gaaaaatgaa attgctttcg acgaaagcaa
     2281 ggcgaatcca gtactgatca aacatgtcaa cgagctgtct ttagcgaagg gcagtcgggt
     2341 atttgtaccg ttgtgcggca agacgcgtga tatttcctgg ttgctctcga agggttatcg
     2401 tgtggctgga gctgaattga gtcaaatcgc cattgagcag ctattcatgg aacttgggct
     2461 acaaccggaa atatcaacag ttggggaagt agagcagtgg agcgcaaacc atgtcgacat
     2521 atttatcggc gatatctttg cgttgtcgag aaagatgctt ggcccagtcg ataccatcta
     2581 tgaccgtgct gcgttagtgg cctttcccga agacatgcgc aatcggtata cggcgcatct
     2641 cacagggatt acacataagg caccgcagct gctcatttgc tatgactacg atcaaagctt
     2701 gatgcagggg cctccctttt cgattcataa cgaggaagtc catcgacatt acgcaggtag
     2761 ttatgagtta acgctcatcg cgagcacaga cgtctctggt ggtttgaagg ggaaatgtgc
     2821 cgccaaagag aatgtgtggc tgctaaagag caagtaatat cggccccgcc cgtgccacga
     2881 aattgattct gacgtcgttc ccttcacaca agaacagtag cctgccccct tttctgcctc
     2941 gaacgacgga agcacaatag cctgtcctct cttcctttct caccttccct tcgttctgta
     3001 agattgccag aagtaccatg catagactag caagagaaaa acgaagttag ggatgagaat
     3061 acttggtgtc atccattgcc agccgtcgaa cttccaatca aatagtggcc agaagaccgg
     3121 tgtcgggaag aacgcatacg aatgcgtggg gatgtcaacc agcacatgca gaccccaggc
     3181 tccgagttcc cagaggggcc gcttcagcag gaaccacacc aagaagaaca cggcgaggaa
     3241 aatgatgaag ctatgggtga cattgtagag atgatgcaca tactgaggga tcgtcgattc
     3301 cggaggcgtg ccgtgactga agtcgggttg ttccgacaag ccgagtatcg cggctaccca
     3361 cagaatcccg aacgagaaaa tatcgggagc cgtgccggtc acaaaggcca gccagaaact
     3421 cgatcgactc ttccgaccaa aggcaatcga accccagaga ccgtgagaga caacatccat
     3481 cagatcattt ccaggcgaag aaacgactat attttacttt ttcttcctgg cccgatccat
     3541 tgtgtggatc tggaatcagt cagaaattac agatctcatc gactctgtcg tcgaaactac
     3601 gacagtggcg gtcatgtcaa tttgacaaga gaatcctgat tctgtcgata gagccccgtc
     3661 cacgactcgt ctataaacct gcacacgaac aacgaacaga tttcatatgc cctccagcac
     3721 gttgccggtc tatccgtggg tgcacaggtc cgctctcaca gcaccttgga cgactaactg
     3781 agagcgtgcc ggccaaacaa ttgggagatg tcaggtatta accggcacgc cccgagaatg
     3841 gttccgacat ctttatctct ccttatcccg agaaggaggc gccaaatatg aagcaagcca
     3901 cccatcctcg attcacgaaa ctgccgtatc tcatattcac cctgctcctg actattggcc
     3961 tctgtgtcct atccggcacg ctgatggccc aggagggtaa ggtcacgccg cttatgtcgg
     4021 aagttctgac ggatatttcc ggcaaggaag gcctgatgat cacagtcgag tatgcgcccg
     4081 gcgggtcgag cccgatccat cgtcacaatg cacatgcatt tgtttacgtg ctggagggct
     4141 ccatcgtgat gcaggtgaag ggcggaaaag aagtcacgct tacaccagga cagacctact
     4201 atgaaaaccc caacgatgtt catgtcgtga gccggaacgc cagcacgacc gcgccggcaa
     4261 aattccttgt ggttctggtc aaagacaagg gcgctccagt cgtcgtgcca gtgaagtgac
     4321 tggggaatga ccgacaagcg agcgtgagcg agagcggcag tccactaccg ctccgaatat
     4381 aatctcgaac agtacttgct cagttacggg cgacaaacga aaggagaatg tatgaagatc
     4441 gttgtgattg gcggcagcgg gctgatcggg acaaaacttg tgaacaggct tcgcaagcat
     4501 gggcatgaag tcgtggctgc atcacctaaa tctggcgtca acaccatcac gggcgagggg
     4561 ttggccgaag cactcgccgg cgctcaggtt gtcgtcgatg tggcaaactc gccttcgttc
     4621 gaggacaagg ccgtgttgaa attct
//
LOCUS       DATIPF010000463         3396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1324390, whole genome shotgun sequence.
ACCESSION   DATIPF010000463 DATIPF010000000
VERSION     DATIPF010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324390"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="VLL06_11490"
     CDS             <1..1434
                     /locus_tag="VLL06_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31633.1"
                     /translation="VRIGISSFIGSCFSTTRSIILLGVGLALSCVVLGAVGIGVIGLA
                     WMIMEAPPSPVYHNLTASPPQAFTDSRKNGYLLLLGFDASAEQDPLQAGYERKAEGSD
                     LQAANACMLGDEGKGTTTGASANVVRGWFTSADPVAMLKPQAAAVRSWAAQESVALRR
                     YQQWLSMPFEDVGYGQILSPNCAHILLAHRLYLAEGFTQDLNSGLGRLETDMGSWRVA
                     LGQSKTLMVKMMAATAVQDDIAIASGLLIRQDLDGETVGRLAKIVRPLDQVELSLRWP
                     MQSHLAWATKSVTTSVKNDKTNERPLHVAVAAAMPLPIQRRSNAYADYYEAASKAVAE
                     GRYTSLPKSSTFIRTPADSAMDYLANPIEHIIGIEPLPSWDSYVGRMVETDARLRLAS
                     LQAWIRRGPQEGNVLTRLAKAGQAHYDPFTGLPMLVDQQKGVMYSVGHDGKDQEGDPL
                     HDVVATIPKTQSVMFESSRSAPSPRSK"
     gene            1812..3296
                     /locus_tag="VLL06_11495"
     CDS             1812..3296
                     /locus_tag="VLL06_11495"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSS31634.1"
                     /translation="MAPSQHSSWKNLDVQQGKTFPSTVPTQIVSNEEFAPFDQTADQA
                     RAEQMAMAMIERSAARRGLTRRDFLKTTGGIAGALLAINSVFGRFFDIEEVELFESAA
                     FAEQQGVPYFIFDAQTHYVSSQYDPTGAEDRRKGGVSKQALLSMRRRIREAGLNPKLA
                     DDKGTLDDLSWRNFVKEVFLDSETTIGLISTPPGPYPQEAVVPPREMAHIRDEVNRVA
                     GSQRMLAHGLVTPQLGTADLDFMAMQAETLKVDAWKGYTGSCPKGFDRGWWMDDERIA
                     YPMLEQARKLNVKRVCVHKGLPLGPVPDYNHPKDLIKAAKDFPDIDFLVYHSGLKTTA
                     TVDEVFAKTGEIPWTTEFCRMKQAQPGIKNIYMELGSTFAQLVTTHPAVCAHVLGQII
                     EAFGVDHVLWGTDSIWYGTPQWQIEAFRRFQIPDQLIDRHRYQSLTRQVKEQIFGLNA
                     ARIFGIDVSAKRHEVSKDALGQLRMSYLGEGPEPSHRVYGWVAG"
BASE COUNT          740 a          949 c          978 g          729 t
ORIGIN      
        1 gtccgcattg ggatctcgtc gttcatcggg tcctgttttt ccacaacgcg atcaatcatt
       61 ttgttgggtg tggggttggc actctcctgt gttgtgctgg gggccgtcgg catcggagtg
      121 ataggattgg cttggatgat catggaagct cccccgtcgc cggtctatca caacctgact
      181 gcgagccctc cccaggcatt cactgattca aggaaaaatg ggtatctttt gctgctcggc
      241 ttcgacgctt ctgcggaaca ggacccactc caagctgggt acgagcgcaa agcggaaggc
      301 agcgatctcc aagctgcgaa tgcctgcatg ctcggcgatg aggggaaagg aacgacgaca
      361 ggggcttccg ccaacgtggt ccgtggatgg ttcaccagtg ccgacccggt ggcgatgctg
      421 aagccgcagg ctgccgcggt acggtcttgg gcagctcaag aatcggttgc cctcaggcgc
      481 taccaacaat ggctttcgat gccatttgaa gatgtcggat acggacaaat tctgagcccg
      541 aactgtgccc atattctgtt ggcccatcgt ttgtatctgg cagaagggtt tacacaggat
      601 ctcaacagcg gtctcggacg acttgagact gatatgggat cctggcgcgt tgcactgggg
      661 cagtcgaaga cgctgatggt caagatgatg gcagcgacag cagtccagga cgatattgcc
      721 atcgcatccg ggcttttgat ccggcaggat ctcgatgggg aaacagtcgg tcgtcttgcc
      781 aagatcgtcc gtcccttaga tcaggttgag ctctccctcc gatggccgat gcagagccat
      841 ctcgcatggg caacgaagtc agtgacgacg agtgtgaaga acgataagac caacgagcgt
      901 cctcttcacg ttgccgtggc agcagcgatg cctcttccca tacagcggcg atccaatgcc
      961 tatgcggact attacgaggc agcaagcaaa gcggtggcgg aagggcgcta caccagcttg
     1021 ccgaagtcgt ccacctttat caggactccc gctgacagtg cgatggacta cttggcaaat
     1081 ccgatcgagc acatcatcgg aatcgagccg cttccctcat gggactccta tgttgggcgt
     1141 atggtagaga ccgatgcacg gcttcgtctg gcgagcctgc aagcgtggat tagacgaggg
     1201 ccacaagaag gaaatgtact cacgcgcctt gcaaaggcgg gacaggccca ctacgatccc
     1261 ttcaccggtc ttcctatgct ggtcgatcag cagaagggtg tgatgtatag tgttgggcat
     1321 gacggcaagg atcaggaagg tgatcccttg cacgatgtcg tcgcaacaat tcccaaaact
     1381 caatcggtca tgttcgaaag tagccgctcc gcgccctcgc ctcggtccaa gtgatccatt
     1441 ggatctttcc gtggtcaaaa acttctgacg cctcagtcca ttgaatcaac cggtctcctg
     1501 tcacactctc cccagcctac gcacggattg tggtttccgc agcttgctcg ctcgatgccg
     1561 gccggcatcg agcatcgtgt attccgcatt gcgcaggtca ccactcgcga taccgatgag
     1621 gcgatggatc tcgtttacgt cgagagatca gtcgcatcct gatggtcata cctcagcaac
     1681 ggccattttc tccgggccgg tcatcgagtc tcggtcctct caggtaccat gatccgagcc
     1741 cctaggttgc ttgacactgc cggtggcatt ccacagaatc acccacaggc ctgtgcagag
     1801 gacgattcgc catggcgcct tcccaacatt ccagctggaa gaatctcgac gttcaacaag
     1861 gcaagacctt tccctctacg gtgccgaccc agatcgtctc gaacgaggaa tttgcaccct
     1921 tcgatcagac tgcggaccag gctcgtgccg aacagatggc catggcaatg attgagcgct
     1981 ccgctgctag gcggggcctg acccgtaggg attttctcaa aacgactggc ggaatcgccg
     2041 gtgcactgct ggccataaat tcagtcttcg ggcggttttt cgacattgaa gaagtcgaat
     2101 tattcgagtc ggcagctttt gcagaacagc agggagtacc ctacttcatc tttgacgcgc
     2161 agacgcatta tgtgagctct cagtatgatc caacaggtgc ggaggatcgc cgcaaagggg
     2221 gtgtgtccaa acaggcattg ctgtctatgc gtcgtcgcat tcgcgaagcg ggactcaacc
     2281 ccaagctggc ggacgataag ggcacgctcg acgatctgtc ctggcgcaac ttcgtgaaag
     2341 aagtctttct cgacagtgag acgacgatcg gactcatcag tacgccccct gggccctacc
     2401 cgcaagaggc cgttgtgcca ccacgcgaga tggcgcacat tcgagatgag gtgaatcgag
     2461 tcgctggttc gcagcgcatg ctggcccatg gccttgtcac accacaactt ggcacggcgg
     2521 atcttgactt tatggccatg caggccgaga ccctcaaagt cgatgcttgg aaaggctaca
     2581 ccgggtcttg tcccaaggga ttcgaccgcg gctggtggat ggacgatgag cggattgcct
     2641 atcccatgtt ggaacaggcg cgcaagctca acgtgaagcg ggtctgtgtc cacaagggat
     2701 tgccgctcgg acccgtgcca gactacaacc atccaaagga cttgatcaag gcggccaagg
     2761 attttcccga catcgacttc ctggtctatc actcggggct gaaaactact gcaacggtag
     2821 atgaggtatt tgcaaaaacg ggcgagattc cctggaccac cgagttctgc cgtatgaagc
     2881 aggcccagcc tggaatcaag aatatctaca tggaacttgg ctcgaccttt gcgcagctcg
     2941 tcacgactca tccggcagtc tgcgctcatg tgcttgggca gatcattgag gcgttcggcg
     3001 tcgatcatgt gctctggggc acggactcca tctggtatgg taccccgcaa tggcagatcg
     3061 aagcattccg gcggtttcag attcccgacc aactgatcga caggcatcgc tatcagtccc
     3121 tgacacggca ggtgaaggag cagatctttg gactcaacgc cgccagaatt ttcgggatcg
     3181 atgtgagtgc caaacgacac gaggtgtcca aggatgcact gggtcaactc agaatgagtt
     3241 atctcggaga agggccggag ccgagtcacc gagtctatgg atgggtagcg gggtgagaag
     3301 aaggtgccca aatcgctcct tgctcgcgca tgcgcacgat cagaatgcgc tcggtgaaca
     3361 cgcgcagtca gagcgatccg tgcaccttct tcagcg
//
LOCUS       DATIPF010000464         6984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1324490, whole genome shotgun sequence.
ACCESSION   DATIPF010000464 DATIPF010000000
VERSION     DATIPF010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6984
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324490"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VLL06_11500"
     CDS             complement(<1..939)
                     /locus_tag="VLL06_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSS31635.1"
                     /translation="MKLVRAWLIPFTLVSGGIIIGIVVASNMGWLPTGTAGPEPTPSP
                     IVRPVVTAPQLPMAGGDGKNFVEIAKSVKPAVVNIAASRSGKSGDSPHGSLEDPFFRK
                     FFGDEFFKRDAPQREPKERGQGSGVIVEANGLIITNNHVVNKADEIRVFLSDKREFKG
                     KLIGTDAKTDIAIVKIEATGLPTVPWADSDQLEVGEYVLAVGSPFGLTQTVTMGIVSA
                     VGRASMGIAEYEDFIQTDAAINPGNSGGALVNVRGELVGINTAIFSQIGGSMGIGFAV
                     PSNLSRAVMDQLVRSGKVVRGWLGVAIQDLTPELATQ"
     gene            complement(1007..1930)
                     /locus_tag="VLL06_11505"
     CDS             complement(1007..1930)
                     /locus_tag="VLL06_11505"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HSS31636.1"
                     /translation="MADRVQEILSWYSSDNAGTKTNIARLLRHGKLAGTGKLVILPVD
                     QGFEHGPARSFASNPGGYNPLYHFQLAIDAGCNAYAAPLGFLEAGAAEFAGRIPLILK
                     LNNHDVLHDEKDPLPSVTGSVNDALRLGCTAVGFTIYPGSSHCNAMYQQLREIAENAK
                     DCGLAVVVWSYPRGSELSKEGETAIDVVGYAAQIAAQLGAHVIKVKLPTAHLEQAAAK
                     KVYESAKIPIATLAERVRHVVQSSFDGRRIVIFSGGAKSEDANVFEEARAIREGGGFG
                     SIIGRNSFQRPKAEAMKFLNTIMAIYAGEIQ"
     gene            complement(1979..3073)
                     /gene="fbp"
                     /locus_tag="VLL06_11510"
     CDS             complement(1979..3073)
                     /gene="fbp"
                     /locus_tag="VLL06_11510"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HSS31637.1"
                     /translation="MPALFKTVILASIMGQFPLTLGRFITRKQASQPGATGEFSNLLD
                     QIGLVGKLIAQDLRRAGLINILGTTGEINVQGETVKKLDGIANETFLKVFDQSGLVCA
                     LASEEMDKPVLFPEHWPQAKYMLLFDPLDGSSNTDCNMPLGAIFSVVKASHKDRMPTE
                     DELVRKGTDQVAAGYLLYGPSTMLVYTVGQGVHGFTLDPGIGEYILSHERVCIPDRGK
                     IFSTNEGNYHKWTAGTKQYVEYLKLPDTATGRPYSGRYSGCLVADVHRILLDGGIYLY
                     PGELRRPEGKLRLLYEANPLAWVVEQAGGRASTGTGRILDVEPKQLHQRVPLIIGSAN
                     DVRDAEEFIQDKREPRMPGHTREILVGPGS"
     gene            complement(3090..4037)
                     /locus_tag="VLL06_11515"
     CDS             complement(3090..4037)
                     /locus_tag="VLL06_11515"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HSS31638.1"
                     /translation="MLKKSASFVLASLRGSTYRSVRLASSLAAALLGRLFEHPARCSP
                     AILPARPPIGDVRCPAWVGLVCLISCAMIGAVSWAGSGEPSSTRPFQAGEQLTYDISW
                     MNITAGTAVMAVNAADTDGDRPLTKLLTTAQSRPSITTFFPVDNRVESIVDSATLLPE
                     HLNFKRREGKKKEDIEYTFHQKEGRVTVVKGGTTETFEMPPGTQDVISCLYYARSGLS
                     FQPGSVMTMNVYHDKKNRKLEVRVEEIETLSGPWGEVETARVLVVMPFQGLFLNRGNI
                     RVWFTTDDRRIPVRMKAKVIIGSIVADLVSGWQASSPAP"
     gene            complement(4042..5442)
                     /locus_tag="VLL06_11520"
     CDS             complement(4042..5442)
                     /locus_tag="VLL06_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HSS31639.1"
                     /translation="MGLFREYDLRGIVGQELTELIAEQVGRAYCTYVQDRGVKTISVG
                     RDGRLSSPGLYKALVRGLLAGGLHVVDIGICPSPLVYFSLFQLPVDGGIMITGSHNAA
                     EYNGFKICIGKDAIHGEEIQALRAVMEAGSFVSGTGQLSEHPIIPDYLAYIKKAFSHV
                     DAKRLHVVIDCGNGVAALVAKQALELLGCSVTGLYCDLDGRFPNHHPDPTVLENLDDL
                     IETVKQHKADVGIGYDGDADRIGTIDEQGNVLWGDRLMVVYARDILATKPGSTIISEV
                     KSSQSLYDDIAKRGGRPIMWKTGHSLIKAKMKVEHAVLAGEMSGHMFFADRYFGYDDA
                     IYASCRLVEILAKTTRSLSSLVADLPKTSVTPEIRVDVSDSIKFELVRQVQDRLADCA
                     KTREELSQAKLVIREVVTIDGVRAIFDDGWGLIRASNTQPALVLRFEATSPDRLVAIQ
                     TAIEGELAKAKQALGC"
     gene            complement(5461..6942)
                     /locus_tag="VLL06_11525"
     CDS             complement(5461..6942)
                     /locus_tag="VLL06_11525"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HSS31640.1"
                     /translation="MASLKRKTSNLKLQNHLYPVIMAGGSGTRFWPLSRQLFPKQLLR
                     IMGDETLIQQTMRRVVCASTPNRVIISTNPAQAESIRVQLNEWKDALKDNFVLEPEGR
                     NTAPAIALVALEVVRRDPDAIMVVVPADHIVKGQRDFDAAVSLASNLAAQGLLVTFGI
                     QPIRPETGYGYIKPNRKTILAKQGKLKGHPVARFVEKPNATKAAQYLKAGGYYWNSGM
                     FVWRAATILEEIRRHQSALFRGIEKIGQLMQQHASRSAIDEEYRQLPSVSIDTGVMEK
                     STKAAVVPVTFRWSDVGSWGSLDEVASKDKTGNVVVGRVVDIDSQRSIVYGDRRVVAT
                     IGLRDMVVVDTPDATLVCPKSRAQEVKNIVEILKQQGAPEHLEHLTVHRPWGSYTVLE
                     EGHGYKVKRVTVKPGGRLSLQMHHKRSEHWVVITGTARVTRGDEVFDLQVGHSTGIPV
                     ETPHRLENPGGETLHIIEVQNGPYLGEDDIVRFKDDYGRTATR"
BASE COUNT         1510 a         2107 c         1773 g         1594 t
ORIGIN      
        1 ctgtgtggcc aactcagggg tcaaatcttg gatcgcaaca ccgagccatc cacgaacgac
       61 cttccccgac cgtaccaatt gatccatcac ggcacgcgac agattgctgg gtaccgcgaa
      121 cccaatgccc atgcttccgc cgatttgact gaagatcgcc gtattgattc cgactaattc
      181 cccccgcaca ttgactaatg ccccgccgga attgcctgga ttgatcgccg catcggtctg
      241 gataaagtcc tcgtattcgg cgatacccat actggctcgt ccgactgcgc tgacgatccc
      301 catcgtcaca gtctgggtga gcccaaacgg gctccccacg gccaatacat actccccgac
      361 ttccagttga tctgaatcag cccatggaac ggtcggaagc cctgtcgctt caattttgac
      421 aatcgcgata tcggtttttg catcggttcc gatcagcttg cccttaaact cccgcttgtc
      481 cgataagaag acccgaatct catcggcctt gttcacgaca tgattgttgg tgatgatcaa
      541 cccgttggcc tctacaatga ccccagaccc ctggccccgc tctttgggct ctcgctgggg
      601 tgcatcacgc ttaaaaaact catcgccgaa gaacttgcga aaaaacgggt cttccagcga
      661 accgtgcgga ctgtctccgg attttccgct tcgtgacgcc gcaatgttga cgaccgccgg
      721 cttcaccgat tttgcgatct ccacgaagtt tttcccatct ccccccgcca tcggtagctg
      781 tggcgcggtc actactgggc gaacaatcgg gctgggagtc ggctcaggcc cggccgtgcc
      841 ggtcggcaac caacccatgt tggacgccac cacaatcccg atgatgatcc ctccgctgac
      901 caaggtaaat ggaatcaacc acgcacgaac gagtttcata ggtgttcctt gctcttcatc
      961 tctcgtgacc cgagtgattc acacacaatc gatctatcct attgcgtcac tggatctcgc
     1021 cggcatagat cgccatgatc gtattgagga acttcatggc ctccgcttta ggccgttgaa
     1081 acgaattgcg cccgatgata gagccgaaac cgccgccctc acgaatggcg cgcgcctcct
     1141 caaacacatt cgcgtcctcg ctcttggccc ctcccgagaa aatgacgatc cggcgcccat
     1201 cgaacgagct ctgtaccaca tgcctgactc gctctgctag cgtggcgatc ggaattttgg
     1261 ctgattcgta caccttcttc gctgctgcct gttcaagatg cgccgtgggg agcttgactt
     1321 taatgacatg ggcgccgagc tgagccgcaa tctgggccgc atagcccacc acatcgatcg
     1381 ctgtctcccc ttccttactg agttccgacc cgcgcggata cgaccacacg acgaccgcca
     1441 acccacagtc cttggcgttt tctgcaatct cgcgcaactg ttgatacatg gcattgcagt
     1501 gcgaggaccc aggatagatc gtgaaaccaa ctgcggtaca gcctaaccgc agtgcatcgt
     1561 tcacgctgcc ggtgaccgag ggcaagggat ctttttcgtc atgcaagaca tcgtggttat
     1621 tcagtttgag aatcagtgga atccggcctg cgaattcagc ggcaccggct tcgagaaacc
     1681 ccagcggagc tgcgtaggca ttgcaaccgg catcgatggc caattgaaaa tgatagaggg
     1741 gattgtaccc tcctggattg gaggcaaagc tccgggccgg gccatgctcg aatccttggt
     1801 ccacgggtaa aatcaccagc ttgcccgtcc ccgccaattt tccatggcgt aatagacgtg
     1861 cgatattcgt ttttgtgcct gcgttgtcac tactgtacca gctcagaatc tcttggactc
     1921 gatcagccat gcccaacctc cgtggattcg tgaaacgtta tgcgttcaac gtcgactgtc
     1981 aagaacctgg gccaaccaat atttctctcg tatgacctgg catgcgcggt tcacgtttgt
     2041 cttggataaa ctcctctgcg tcgcgcacgt cgttcgcact gccgatgatc aacggcaccc
     2101 gttgatgcag ttgcttgggc tccacgtcga ggatgcgtcc agtgccggtg ctggctctcc
     2161 ctcccgcttg ttccacgacc catgccaagg gattggcttc gtatagtaac cggagcttac
     2221 cctctggcct cctcaactcg ccgggataga gatagatgcc accatcaagg agaatccggt
     2281 gcacgtcggc taccagacaa ccggaatagc gtccactata gggccggccc gtggcggtat
     2341 ccggaagctt gaggtactcc acatactgct tcgtgcctgc cgtccattta tgatagttgc
     2401 cctcgttcgt actaaagatc ttgcctcgat caggaatgca gacccgctcg tgggacagaa
     2461 tatactcgcc gatgccggga tccaatgtaa acccgtggac tccctgtcca accgtataga
     2521 ccagcatagt gcttggccca tagagcagat accccgcggc tacctggtct gttcccttgc
     2581 ggaccagttc gtcctcggtc ggcatccggt ctttatgcga ggcctttacg acggaaaaaa
     2641 tcgcgccgag cggcatattg cagtccgtgt ttgatgaccc atcgagagga tcgaacagca
     2701 gcatgtactt ggcctgcggc caatgctccg gaaataggac cggtttgtcc atctcctccg
     2761 acgccagagc gcacacgagt ccactctggt caaatacctt caagaaggtt tcgtttgcga
     2821 tcccatccag tttcttgaca gtctcaccct gaacattgat ctcaccggtc gttcccagga
     2881 tattgatgag acccgcacgt ctgaggtcct gcgcaatgag ttttccaacc aggccgatct
     2941 gatccaatag attggaaaat tccccggtgg ctcccggctg gctcgcctgc ttcctggtga
     3001 taaatcggcc taaagtaaga ggaaactgtc ccataataga ggcgagtata accgttttga
     3061 acagggcggg caacgaaagg ctggttccct catggggctg gtgatgaggc ctgccatccg
     3121 ctgacgagat cggccacaat cgaaccgatg atgactttgg ccttcatgcg gaccggaatg
     3181 cgccgatcgt cggtggtaaa ccacacccgg atgttgcccc gattgaggaa gagtccttgg
     3241 aacggcatga cgaccaacac acgagcggtt tccacctctc cccacggacc gctgagcgtc
     3301 tcaatctcct caacccgcac ctccagtttc cggttctttt tatcgtggta tacgttcatc
     3361 gtcatcacag agcctggctg gaacgataat ccgctgcgtg catagtagag acaagagatg
     3421 acatcttgcg tgcctggcgg catctcaaag gtttcggtcg ttccgccttt caccacagtc
     3481 acccgtccct ctttctgatg aaatgtgtat tcgatatctt ctttcttctt cccttctcgc
     3541 cgcttgaagt tcaagtgctc cggaaggagc gtggcggaat ccacgatcga ctcgacccgg
     3601 ttatccacgg gaaagaatgt ggtgattgag ggcctcgact gcgccgtggt gagcaatttg
     3661 gtcagcggtc gatctccgtc cgtatcagct gcattgacag ccatcaccgc tgtaccggct
     3721 gtgatattca tccaagaaat atcgtaggtg agctgttccc cggcttggaa gggtcgcgtt
     3781 gaagaaggct cgccagaccc tgcccatgag accgcaccaa tcatggcaca agatataaga
     3841 cacaccagtc cgacccaggc gggacaccgc acatctccta tagggggtct cgctggtagg
     3901 atagcaggag aacaccttgc aggatgctca aaaagtcggc ccagcaaggc cgcagcgagc
     3961 gaagaggcga ggcgtacgct tcggtacgtt gagcctctga gcgaagcgag aacgaagctg
     4021 gcggactttt tcagcatcct gctagcagcc aagggcttgt ttggccttcg ccagttcgcc
     4081 ttcgatcgct gtttggatgg caaccaatcg atccggggag gtggcttcaa accgtaacac
     4141 gagggccggc tgggtattgg aggctcggat caggccccac ccatcgtcga agatggcacg
     4201 caccccatcg atggtgacga cctcgcgaat caccagcttg gcctgactaa gctcctcacg
     4261 ggtcttcgca cagtctgcca gccggtcctg cacctgtctg accaactcaa acttgatgga
     4321 gtccgacacg tcgacccgga tctccggagt aaccgacgtc ttcggaaggt ccgccaccaa
     4381 cgaagagagc gatcgagtag tcttggccaa aatctccacc agccggcagg aggcataaat
     4441 tgcgtcgtca tacccgaagt accgatcggc gaagaacata tgtccggaca tttcgccggc
     4501 caagacggcg tgctcgactt tcatcttcgc cttgattaag gagtgaccgg ttttccacat
     4561 gatcggtctc cctccccgct tcgcaatgtc atcatagaga ctctgtgatg atttgacctc
     4621 tgagatgatg gtactccccg gcttggtcgc cagaatatcg cgcgcataga cgaccatcag
     4681 gcgatcgccc cacaacacat tgccttgctc atcgatggtc ccaatgcgat cggcatctcc
     4741 gtcgtagccg atcccgacat cggctttatg ctgtttgacg gtctcgatga gatcgtcaag
     4801 attttctaag acggtgggat caggatgatg gttgggaaat ctgccgtcga gatcgcaata
     4861 gagccccgtg acgctgcagc ccaagagctc caacgcctgt ttcgccacca aggctgcgac
     4921 accatttcca cagtcgatga cgacatggag gcgtttcgca tcgacgtggc tgaaggcctt
     4981 cttgatatag gccagataat cgggaatgat tgggtgttcc gataactgtc cagttccaga
     5041 aacaaaagaa ccggcctcca tcaccgcgcg aagcgcctgg atctcttccc catgaatcgc
     5101 atctttacca atgcagatct taaatccatt gtattccgcc gcattgtggc tcccggtgat
     5161 catgatgccg ccgtcaaccg gcagctgaaa tagagagaaa tacacgagcg gagaggggca
     5221 aattccaata tccaccacat gcaatccacc ggcaaggagt cctcgtacaa gagccttgta
     5281 gagtcctggt gaactcagac gtccatcgcg cccgaccgag atcgtcttca ccccacggtc
     5341 ttgcacatag gtgcagtagg cacgaccaac ttgttcggca atcaattcgg tcagttcttg
     5401 cccgacaatg ccgcgtagat catattctcg gaaaagaccc atcgtcgatc ctcgctctcc
     5461 ctatctggtc gcagtccgcc cataatcatc tttgaatctg acaatgtcgt cctcgcctaa
     5521 atagggccca ttctggactt cgatgatatg gagggtctcg cctccaggat tctctaaacg
     5581 atggggcgtt tccaccggaa tgccggtgct gtgtccaact tgaagatcga acacctcatc
     5641 ccccctggtc acgcgggccg ttccggtaat cacgacccaa tgctcgctgc gcttgtggtg
     5701 catctgtaac gataaccggc cccctggctt caccgtgaca cgcttcacct tgtaaccgtg
     5761 gccttcttcg agcacggtat aggaacccca gggccgatgg acggtgagat gttctaaatg
     5821 ttctggtgca ccttgttgct taagaatctc cacgatattt ttgacttcct gggcacgcga
     5881 cttgggacac acaagcgtag catcgggcgt atctacgacc accatgtccc tcagaccgat
     5941 cgtggccacg actcgtcgat ctccgtagac gatcgatcgc tgactgtcga tatcgaccac
     6001 gcgaccgacc acgacattgc cggtcttatc cttcgatgcc acttcatcca aactgcccca
     6061 acttcccacg tccgaccatc gaaaggtgac cggcacgact gcggccttcg tggacttctc
     6121 catcacccct gtatcgatcg acaccgacgg caattgtcga tattcctcat cgatcgcgga
     6181 tcgcgatgca tgttgctgca tcagctgacc aatcttctca atcccgcgaa agagagccga
     6241 ttggtggcga cggatctctt cgagaattgt tgcggcacgc catacaaaca tcccgctgtt
     6301 ccagtaatag cccccggcct tgagatattg cgccgctttg gtcgcattcg gcttctcgac
     6361 gaatcgagcc actggatgcc ctttcagctt gccctgtttt gccaggatgg tcttccggtt
     6421 tggcttgata tagccgtacc ctgtttcagg acgaatcggc tgaatgccaa aggtgaccaa
     6481 tagaccttgt gccgcaaggt tcgaggcaag ggacacggcc gcatcgaagt cgcgctgacc
     6541 tttcacaata tggtcagccg gcaccaccac cataatggca tcaggatccc gacggaccac
     6601 ttctaacgcc accaaggcga tcgcaggtgc cgtattgcgc ccttcaggtt ccagcacgaa
     6661 attatctttg agcgcgtcct tccattcatt cagctggacg cgaatggact ctgcttgggc
     6721 aggattcgtc gatatgatca cgcggtttgg cgtcgaagca cagaccaccc gacgcatcgt
     6781 ctgctgaatc aacgtttcat ctcccataat ccgcagtagc tgtttaggaa atagctgtcg
     6841 actgagcggc caaaatctcg tgccgctgcc accggccatg atcacgggat acagatggtt
     6901 ctgcagcttg aggttcgagg tttttcgttt gagagaagcc atatgctgag tctcctaccc
     6961 tatacgcgtg ctccggaact tgga
//
LOCUS       DATIPF010000465         4654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U15843
            k141_1325308, whole genome shotgun sequence.
ACCESSION   DATIPF010000465 DATIPF010000000
VERSION     DATIPF010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936554
            Sequence Read Archive: SRR9656168
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:00:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,312
            CDSs (total)                      :: 2,281
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325308"
                     /isolate="SMAG_U15843"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VLL06_11530"
     CDS             <1..395
                     /locus_tag="VLL06_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HSS31641.1"
                     /translation="DLLNPVITIPSGALKLDIAQDGTVSVLLPGVTQASQVGQIQLNR
                     FDNPSGLVAMGNNLFLDSFASGPPLQGTGGFSTGFGTIQQGFLESSNVNLAEEMVNMI
                     IAQRSYEINSKTIQASDEMMSIANNLKR"
     gene            421..1182
                     /gene="flgA"
                     /locus_tag="VLL06_11535"
     CDS             421..1182
                     /gene="flgA"
                     /locus_tag="VLL06_11535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HSS31642.1"
                     /translation="MIVSRSFLQSTLVAMMCLVPLLTEAASLQQIQTVSGGGVVGSQL
                     PPEHIQRSILQLLELEMAGKVRAVHTVMVDPVDPIKMPSGSIDLKVSPNGLAEGLGRR
                     TFHIQINTNGKLWRTIDAIADVSASIDAVVPTRVLQIDSVIDAEDVTVHRIKLTDLDH
                     QLFTNVADVIGKSVARPLQTNNPIRLGMVKKPYAVRKGDRVSIEARRGGLSIQTVGVT
                     KASGELGQSITVANVDSGKELRAKVIGPGAVRVEY"
     gene            1307..1981
                     /locus_tag="VLL06_11540"
     CDS             1307..1981
                     /locus_tag="VLL06_11540"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HSS31643.1"
                     /translation="MAVTMLTVALLTNGCTSSSEKVVSTLTVPSLPPPKTVGSLWQEE
                     NGRAYLYEDMRAMRVGDILTIKIVENHKGSKSADTSAERDSTIQNSLAGTAVGYVGIP
                     GIRLGGEARRGLGIDASASNKFGGKGATNREGTLTGTISVIVTEVLPNGDLRVEGRRE
                     VTVNSEKQMMTIGGLVRRVDVDTKNTVLSSAIADAKIVYSGLGVLDDVQRPGWLVRVL
                     DWVSPW"
     gene            2274..3407
                     /locus_tag="VLL06_11545"
     CDS             2274..3407
                     /locus_tag="VLL06_11545"
                     /inference="COORDINATES: protein motif:HMM:NF014206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HSS31644.1"
                     /translation="MIRVRMGLGLLVLGSLLWAIPANAVRIKDVGSIEGVRDNQLIGY
                     GLVVGLDRTGDQVIGGQFTIQAMMSMLNKMGINLVINPIQLLTRNIASVIVTAKLPPF
                     AKPGLTVDVLVSSMANARSLQGGTLLLTPLKAANQQVYAVAQGPVSIGGFLGGTGGAG
                     GSTITKNHQAAGTVPGGAIIEKDAGIEIESWENVSVLLRQPDFTTAIRTTEAIDGVFG
                     KGSATAVSAGLVRASIPATFRGRVVEYIATIEGLDVNVDVAAKVVVNERTGTVVLGEH
                     VRLSTCAVSHGNLTISIKNTQNVSQPPAPLIGSTGGQTTVTEDVHTEVKEQESRLIVM
                     DETVTLGDVVGALNAVGVTPRDLVAILSALKAAGALQANLDVI"
     gene            3495..3803
                     /locus_tag="VLL06_11550"
     CDS             3495..3803
                     /locus_tag="VLL06_11550"
                     /inference="COORDINATES: protein motif:HMM:NF021638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HSS31645.1"
                     /translation="MPQVPPIGIQEQQGKLADLKDAAHEFEAYFISHMLKVMRETVPK
                     GALENKGGAYFYSFYDQEIGRLASEAGGLGLAKMIHEYMEKNAQPLKFSEPIADTIVD
                     "
     gene            3875..4177
                     /gene="flgM"
                     /locus_tag="VLL06_11555"
     CDS             3875..4177
                     /gene="flgM"
                     /locus_tag="VLL06_11555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HSS31646.1"
                     /translation="MQISGHGKPDQLARLLLGAQGTDGVSAKTKTQQDGRQDRVQISD
                     RAKEMQRIKALADQPDTAREERIERISRSVDAGTYTVSGKQVADALIRQTLTDAVL"
     gene            4396..>4654
                     /locus_tag="VLL06_11560"
     CDS             4396..>4654
                     /locus_tag="VLL06_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS31647.1"
                     /translation="MQDIMTDIQQLDHILCREEAQCDALAHNIQRERQALRTLKLTDM
                     AEVNHQRAGLLDDLLLLEAERTAWVARLANRWNLAPSAISLS"
BASE COUNT         1076 a         1194 c         1393 g          991 t
ORIGIN      
        1 gtgatctcct caatcccgtg atcacgatcc cgtcaggggc gttgaagctc gacatcgctc
       61 aagatggcac cgtctccgtg ctgctccccg gtgtgacgca agcgtcgcaa gtggggcaga
      121 ttcaattgaa tcgcttcgat aatccgtccg gactggtcgc gatgggcaac aatctctttc
      181 tcgatagttt cgcctccggc cctccattgc aagggaccgg tggtttctcg accggatttg
      241 gaacaatcca acagggcttt ctggaaagtt ccaacgtgaa cctggccgag gaaatggtca
      301 acatgattat tgcgcaacgg agctatgaaa ttaactcgaa gacgattcaa gcatcggatg
      361 aaatgatgtc gatcgccaac aatctgaaac ggtaagtgcg aggcagggag gaatgtttcc
      421 atgatcgtat ctcgttcatt cttgcagtca accctggtgg ccatgatgtg tcttgtcccg
      481 ctattgacag aggctgcttc gttgcaacag atccaaacag tatccggtgg aggagtggtg
      541 ggttcccagt tgcctccgga gcacatccaa cgaagcattc tgcaattact cgagctagag
      601 atggcgggaa aggtccgtgc cgttcatacg gtcatggtag atccggttga tcctataaag
      661 atgccatccg gcagcatcga cttgaaggtt tccccgaacg gacttgcaga gggtctcggg
      721 cgccggacat ttcatatcca gatcaacacg aacgggaaac tctggcggac gattgacgcc
      781 attgccgacg tgtcggcctc catcgatgcc gtggtcccga cacgagtcct gcaaatcgac
      841 agcgtcatcg atgcagagga tgtcacggtt catcggatca aactcacaga cttggaccat
      901 cagctgttca cgaacgtggc ggatgtcatc gggaagagtg tcgcccgtcc gttgcagacc
      961 aacaacccga ttcgtctagg gatggtgaag aagccatatg cggtgcggaa gggggatcgc
     1021 gtatccatcg aagcgaggcg cggagggctt tccattcaaa cggtcggcgt gacgaaagca
     1081 agcggagaac ttgggcagtc catcacggtg gccaatgtcg attcaggcaa agaactgcgt
     1141 gcgaaggtga ttggaccggg agccgtgcgt gttgaatact aacaggatgc tgaaaaagtc
     1201 gttcttctca cccgcccagc ccaggtgtgc cgtgacacac cttttcccac gcttcgttct
     1261 cgcttcgctc agagactcaa cgtacccaag cgtacgcttc gcggctatgg cggtgaccat
     1321 gttgacggtg gcgctcttga ccaatggctg tacttcatcg tcggagaaag ttgtatcgac
     1381 ccttacagtc ccttccttgc cgccaccaaa aacggtggga tctctctggc aagaagagaa
     1441 tggccgcgcc tacctctatg aagacatgcg ggcgatgcgg gttggagata ttctgacgat
     1501 caagattgtt gaaaatcaca aaggatcgaa gtccgccgat acctcggctg agcgggattc
     1561 caccatccaa aacagtctgg ccggcaccgc cgttggatat gttggtattc ccggcattcg
     1621 cctgggcggc gaggccaggc gggggcttgg catcgatgcg agcgcttcga acaaatttgg
     1681 cggcaagggc gccacgaatc gtgaaggaac gttgacggga accatttccg tcatcgtgac
     1741 ggaagttctg cccaatggtg atctccgggt cgaaggacgc cgggaggtca cggtgaatag
     1801 cgaaaagcaa atgatgacaa tcgggggtct tgtgcggcgg gtcgatgtgg atacgaaaaa
     1861 taccgtgctc tcgtccgcca tcgcggatgc caagatcgtg tattcggggc tgggagtgtt
     1921 ggacgacgtc cagcgtccag gatggctcgt gagggtgctc gattgggtca gtccatggtg
     1981 atcaaagacg tgggaactat gaaatacgcc aagagaggga caaggggtat ccactgggat
     2041 cgctggcgcc gcaggatggt catgcgatca ttggagatgc tggtgacgag cggagtcgtc
     2101 cgccgtgcag aggtcgatcc ccctcaacat cttgatgagc gtctggagag tgaaacgagc
     2161 ccggaggcgt cggtattgtc cttgcctgcc gtcaaccttg atgcagaggg ttggttcagc
     2221 cgtttcgttc gtcgacgcgc cgattcacct tttacgcatg tgagacacac gacatgatac
     2281 gagtacggat gggtctcggg ctgttggtat tgggaagcct tctctgggcg ataccggcga
     2341 atgcggttcg tatcaaagac gtgggatcga tcgaaggggt tcgggacaac cagttgatcg
     2401 gctacggcct cgtcgtcggc ctcgatcgaa ccggcgatca ggtgatcggt ggacagttca
     2461 cgatccaggc catgatgtcg atgttgaaca agatgggcat caacctcgtg atcaatccga
     2521 tccagttgct gacgcgcaac attgcctcgg tcatcgtcac tgcgaaactg ccgccctttg
     2581 ccaaaccggg cttgacggtc gatgtgctgg tgtcctcaat ggccaatgcg aggagcttgc
     2641 aaggcggaac cttgttgctc actccgttga aggcggcgaa ccagcaagtg tatgccgtgg
     2701 cgcaagggcc ggtttcaatc ggaggattct taggagggac tggtggagca ggcgggtcta
     2761 ccatcaccaa gaaccatcaa gccgccggga cggtccctgg cggtgcgatt attgaaaagg
     2821 atgccgggat cgagattgaa tcgtgggaga acgtctcggt gcttctgcgg cagccggatt
     2881 ttacgacggc gatccggacg acggaagcga tcgacggggt gtttggcaag gggagcgcaa
     2941 cggcggtgag tgctggactc gttcgagcgt ccattccggc aacatttcgt ggccgggtcg
     3001 tcgagtacat cgccacgatc gaaggattgg atgtcaatgt cgacgtggct gcaaaagtcg
     3061 tcgtgaatga gcgaaccggt accgtggtgc tgggggaaca tgtccgtctc tctacctgcg
     3121 ccgtgtcaca tgggaatctc acgatctcga taaagaatac gcaaaatgtc tcgcagcctc
     3181 ctgctccgtt gatcgggtcc accggcgggc aaaccactgt aacggaagat gtgcacacag
     3241 aggtgaaaga acaggaatcc agactgatcg tcatggatga aacggttacg cttggggatg
     3301 tagtgggagc gttgaatgcg gttggagtga cccccagaga tttggtcgcg atcctatcag
     3361 ccctcaaagc agccggagct cttcaggcta atcttgatgt tatatagata agtaattgat
     3421 agttagaggt tatattgtat cacgatttta agagtattag ctcagtcggt aacagagccg
     3481 agatccagct tggtttgcca caagttccgc caatcggcat ccaggagcag caggggaaac
     3541 tagctgatct caaggatgca gcccatgagt tcgaagccta ttttatctct catatgctga
     3601 aggtcatgag agaaaccgtt ccaaaggggg cgttggagaa caagggggga gcctacttct
     3661 actcctttta tgaccaggaa atcggccgat tggcatccga agcagggggg cttggcttgg
     3721 cgaaaatgat tcatgaatat atggaaaaaa atgctcaacc cctcaagttt tcagaaccga
     3781 tcgccgatac tatagtcgac taggatcatt gccccaccct tcaaagggtg ccagggtgaa
     3841 cttgtgagag acagtacaga gaggagcgat tgttatgcag atctccggtc atggtaagcc
     3901 agatcaactt gccaggctcc tacttggagc ccaaggaacc gatggggtca gtgccaagac
     3961 gaagacccaa caggacggac gccaggatcg agtgcagatc tcagatcggg ccaaggagat
     4021 gcaacgaatc aaggcattgg ccgatcagcc ggataccgct cgggaagagc ggatcgaacg
     4081 gattagccga tccgtcgatg ccggcacgta cacagtgtcg gggaagcagg ttgccgacgc
     4141 tcttattcgt cagacgctta ccgacgcagt tctctagcag gatgctgaaa aagccgaccg
     4201 tctcacccgc ccaactccgg cgcgccgaga cgcgcctttt cccatgcggc gttctcgcat
     4261 cgttcagacc ctcaaccgag acccggccgc ctcaccactt ggcggtgtgc acagacttgg
     4321 tgttccttcg tgttcaggat tcagtcgacc tctggcgcaa cggaagtatc ccttcgggtt
     4381 tgaggagcac ggaccatgca ggacatcatg acggacatcc agcaactcga tcacatccta
     4441 tgccgtgaag aagcgcagtg cgatgcgctg gcacacaata ttcaacgaga acgacaagcc
     4501 cttcgcacgc tgaaattgac ggacatggct gaggtcaatc atcagcgggc cgggctactg
     4561 gatgatcttc tcctcctcga agcggagcga acggcgtggg tggctcggtt agccaatcgt
     4621 tggaatctcg ctccgtcagc catatccctg agcg
//
